LOCUS       DAJVOY010000001        89254 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVOY010000001 DAJVOY010000000
VERSION     DAJVOY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.001"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..791
                     /locus_tag="OQL90_000001"
     CDS             <1..791
                     /locus_tag="OQL90_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1366918.1"
                     /translation="DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQT
                     GVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGVWVDGMGSPSTGD
                     FHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITY
                     DCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQ
                     NGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OQL90_000002"
     CDS             complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OQL90_000002"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1366919.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1758..3785
                     /gene="lpoA"
                     /locus_tag="OQL90_000003"
     CDS             1758..3785
                     /gene="lpoA"
                     /locus_tag="OQL90_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1366920.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3743..4138
                     /locus_tag="OQL90_000004"
     CDS             3743..4138
                     /locus_tag="OQL90_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1366921.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4158..4748
                     /gene="diaA"
                     /locus_tag="OQL90_000005"
     CDS             4158..4748
                     /gene="diaA"
                     /locus_tag="OQL90_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1366922.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4758..5333
                     /gene="yraP"
                     /locus_tag="OQL90_000006"
     CDS             4758..5333
                     /gene="yraP"
                     /locus_tag="OQL90_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1366923.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5453..6004)
                     /locus_tag="OQL90_000007"
                     /pseudo
     CDS             complement(5453..6004)
                     /locus_tag="OQL90_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(6077..6712)
                     /locus_tag="OQL90_000008"
     CDS             complement(6077..6712)
                     /locus_tag="OQL90_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1366924.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6840..7358
                     /gene="yhbO"
                     /locus_tag="OQL90_000009"
     CDS             6840..7358
                     /gene="yhbO"
                     /locus_tag="OQL90_000009"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1366925.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7338..7781)
                     /locus_tag="OQL90_000010"
     CDS             complement(7338..7781)
                     /locus_tag="OQL90_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1366926.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7832..8134
                     /gene="yhbQ"
                     /locus_tag="OQL90_000011"
     CDS             7832..8134
                     /gene="yhbQ"
                     /locus_tag="OQL90_000011"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1366927.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8121..8624)
                     /locus_tag="OQL90_000012"
     CDS             complement(8121..8624)
                     /locus_tag="OQL90_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1366928.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8618..9142)
                     /locus_tag="OQL90_000013"
     CDS             complement(8618..9142)
                     /locus_tag="OQL90_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1366929.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9351..10331
                     /locus_tag="OQL90_000014"
     CDS             9351..10331
                     /locus_tag="OQL90_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1366930.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10340..11218
                     /locus_tag="OQL90_000015"
     CDS             10340..11218
                     /locus_tag="OQL90_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1366931.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11424..12431
                     /locus_tag="OQL90_000016"
     CDS             11424..12431
                     /locus_tag="OQL90_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCS1366932.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OQL90_000017"
     CDS             complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OQL90_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1366933.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OQL90_000018"
     CDS             complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OQL90_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1366934.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OQL90_000019"
     CDS             complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OQL90_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1366935.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OQL90_000020"
     CDS             complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OQL90_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1366936.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OQL90_000021"
     CDS             complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OQL90_000021"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1366937.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OQL90_000022"
     CDS             complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OQL90_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1366938.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OQL90_000023"
     CDS             complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OQL90_000023"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1366939.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OQL90_000024"
     CDS             complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OQL90_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1366940.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21319..24015)
                     /gene="infB"
                     /locus_tag="OQL90_000025"
     CDS             complement(21319..24015)
                     /gene="infB"
                     /locus_tag="OQL90_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1366941.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREA
                     AEKDKVSNQQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMA
                     EENKWTDNAEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKH
                     AESKADREEARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMA
                     VKGSQVIKAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTG
                     AAAEPRAPVVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITF
                     LDTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKI
                     DKPEADPDRVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELK
                     AVRKGMASGAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQE
                     VLEAGPSIPVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKL
                     ENMFANMTEGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETD
                     ATLAAASNAILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPEL
                     KQQIIGLAEVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRF
                     KDDVNEVRNGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24040..25527)
                     /gene="nusA"
                     /locus_tag="OQL90_000026"
     CDS             complement(24040..25527)
                     /gene="nusA"
                     /locus_tag="OQL90_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1366942.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25555..26007)
                     /gene="rimP"
                     /locus_tag="OQL90_000027"
     CDS             complement(25555..26007)
                     /gene="rimP"
                     /locus_tag="OQL90_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1366943.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26214..26290)
                     /locus_tag="OQL90_000028"
     tRNA            complement(26214..26290)
                     /locus_tag="OQL90_000028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26254..26256),aa:Met,seq:cat)
     gene            26638..27981
                     /gene="argG"
                     /locus_tag="OQL90_000029"
     CDS             26638..27981
                     /gene="argG"
                     /locus_tag="OQL90_000029"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1366944.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(27937..29613)
                     /locus_tag="OQL90_000030"
     CDS             complement(27937..29613)
                     /locus_tag="OQL90_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1366945.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPLKGRVDVELLAFVFHILHLRHA"
     gene            complement(30072..30158)
                     /locus_tag="OQL90_000031"
     tRNA            complement(30072..30158)
                     /locus_tag="OQL90_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30122..30124),aa:Leu,seq:gag)
     gene            complement(30173..30505)
                     /gene="secG"
                     /locus_tag="OQL90_000032"
     CDS             complement(30173..30505)
                     /gene="secG"
                     /locus_tag="OQL90_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1366946.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30733..32070)
                     /gene="glmM"
                     /locus_tag="OQL90_000033"
     CDS             complement(30733..32070)
                     /gene="glmM"
                     /locus_tag="OQL90_000033"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1366947.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32063..32911)
                     /gene="folP"
                     /locus_tag="OQL90_000034"
     CDS             complement(32063..32911)
                     /gene="folP"
                     /locus_tag="OQL90_000034"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1366948.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33001..34935)
                     /gene="ftsH"
                     /locus_tag="OQL90_000035"
     CDS             complement(33001..34935)
                     /gene="ftsH"
                     /locus_tag="OQL90_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1366949.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35035..35664)
                     /gene="rlmE"
                     /locus_tag="OQL90_000036"
     CDS             complement(35035..35664)
                     /gene="rlmE"
                     /locus_tag="OQL90_000036"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1366950.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35790..36083
                     /gene="yhbY"
                     /locus_tag="OQL90_000037"
     CDS             35790..36083
                     /gene="yhbY"
                     /locus_tag="OQL90_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1366951.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36240..36716)
                     /gene="greA"
                     /locus_tag="OQL90_000038"
     CDS             complement(36240..36716)
                     /gene="greA"
                     /locus_tag="OQL90_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1366952.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36964..38397
                     /gene="dacB"
                     /locus_tag="OQL90_000039"
     CDS             36964..38397
                     /gene="dacB"
                     /locus_tag="OQL90_000039"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1366953.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38437..39609)
                     /gene="cgtA"
                     /locus_tag="OQL90_000040"
     CDS             complement(38437..39609)
                     /gene="cgtA"
                     /locus_tag="OQL90_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1366954.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39625..40590)
                     /locus_tag="OQL90_000041"
     CDS             complement(39625..40590)
                     /locus_tag="OQL90_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1366955.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40717..40974)
                     /gene="rpmA"
                     /locus_tag="OQL90_000042"
     CDS             complement(40717..40974)
                     /gene="rpmA"
                     /locus_tag="OQL90_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1366956.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40995..41306)
                     /gene="rplU"
                     /locus_tag="OQL90_000043"
     CDS             complement(40995..41306)
                     /gene="rplU"
                     /locus_tag="OQL90_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1366957.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41565..42536
                     /gene="ispB"
                     /locus_tag="OQL90_000044"
     CDS             41565..42536
                     /gene="ispB"
                     /locus_tag="OQL90_000044"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1366958.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42764..43042
                     /gene="sfsB"
                     /locus_tag="OQL90_000045"
     CDS             42764..43042
                     /gene="sfsB"
                     /locus_tag="OQL90_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1366959.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43090..44349)
                     /gene="murA"
                     /locus_tag="OQL90_000046"
     CDS             complement(43090..44349)
                     /gene="murA"
                     /locus_tag="OQL90_000046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1366960.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(44404..44658)
                     /gene="ibaG"
                     /locus_tag="OQL90_000047"
     CDS             complement(44404..44658)
                     /gene="ibaG"
                     /locus_tag="OQL90_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1366961.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44818..45111)
                     /gene="mlaB"
                     /locus_tag="OQL90_000048"
     CDS             complement(44818..45111)
                     /gene="mlaB"
                     /locus_tag="OQL90_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1366962.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45111..45746)
                     /gene="mlaC"
                     /locus_tag="OQL90_000049"
     CDS             complement(45111..45746)
                     /gene="mlaC"
                     /locus_tag="OQL90_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1366963.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45765..46316)
                     /gene="mlaD"
                     /locus_tag="OQL90_000050"
     CDS             complement(45765..46316)
                     /gene="mlaD"
                     /locus_tag="OQL90_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1366964.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46321..47103)
                     /gene="mlaE"
                     /locus_tag="OQL90_000051"
     CDS             complement(46321..47103)
                     /gene="mlaE"
                     /locus_tag="OQL90_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1366965.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47111..47920)
                     /gene="mlaF"
                     /locus_tag="OQL90_000052"
     CDS             complement(47111..47920)
                     /gene="mlaF"
                     /locus_tag="OQL90_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1366966.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGCAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48130..49107
                     /locus_tag="OQL90_000053"
     CDS             48130..49107
                     /locus_tag="OQL90_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1366967.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49121..50107
                     /gene="kdsD"
                     /locus_tag="OQL90_000054"
     CDS             49121..50107
                     /gene="kdsD"
                     /locus_tag="OQL90_000054"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1366968.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50128..50694
                     /gene="kdsC"
                     /locus_tag="OQL90_000055"
     CDS             50128..50694
                     /gene="kdsC"
                     /locus_tag="OQL90_000055"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1366969.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50691..51266
                     /gene="lptC"
                     /locus_tag="OQL90_000056"
     CDS             50691..51266
                     /gene="lptC"
                     /locus_tag="OQL90_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1366970.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51235..51792
                     /gene="lptA"
                     /locus_tag="OQL90_000057"
     CDS             51235..51792
                     /gene="lptA"
                     /locus_tag="OQL90_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1366971.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51799..52524
                     /gene="lptB"
                     /locus_tag="OQL90_000058"
     CDS             51799..52524
                     /gene="lptB"
                     /locus_tag="OQL90_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1366972.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52572..54005
                     /gene="rpoN"
                     /locus_tag="OQL90_000059"
     CDS             52572..54005
                     /gene="rpoN"
                     /locus_tag="OQL90_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1366973.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54028..54315
                     /gene="hpf"
                     /locus_tag="OQL90_000060"
     CDS             54028..54315
                     /gene="hpf"
                     /locus_tag="OQL90_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1366974.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54433..54924
                     /gene="ptsN"
                     /locus_tag="OQL90_000061"
     CDS             54433..54924
                     /gene="ptsN"
                     /locus_tag="OQL90_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1366975.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54970..55824
                     /gene="rapZ"
                     /locus_tag="OQL90_000062"
     CDS             54970..55824
                     /gene="rapZ"
                     /locus_tag="OQL90_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1366976.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55821..56093
                     /gene="npr"
                     /locus_tag="OQL90_000063"
     CDS             55821..56093
                     /gene="npr"
                     /locus_tag="OQL90_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1366977.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56307..56939
                     /gene="yrbL"
                     /locus_tag="OQL90_000064"
     CDS             56307..56939
                     /gene="yrbL"
                     /locus_tag="OQL90_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1366978.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56936..57664)
                     /gene="mtgA"
                     /locus_tag="OQL90_000065"
     CDS             complement(56936..57664)
                     /gene="mtgA"
                     /locus_tag="OQL90_000065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCS1366979.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57661..58314)
                     /gene="elbB"
                     /locus_tag="OQL90_000066"
     CDS             complement(57661..58314)
                     /gene="elbB"
                     /locus_tag="OQL90_000066"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1366980.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58544..60880)
                     /gene="arcB"
                     /locus_tag="OQL90_000067"
     CDS             complement(58544..60880)
                     /gene="arcB"
                     /locus_tag="OQL90_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1366981.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60976..61905)
                     /locus_tag="OQL90_000068"
     CDS             complement(60976..61905)
                     /locus_tag="OQL90_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1366982.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62580..67040
                     /gene="gltB"
                     /locus_tag="OQL90_000069"
     CDS             62580..67040
                     /gene="gltB"
                     /locus_tag="OQL90_000069"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1366983.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67053..68471
                     /gene="gltD"
                     /locus_tag="OQL90_000070"
     CDS             67053..68471
                     /gene="gltD"
                     /locus_tag="OQL90_000070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1366984.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68655..69134
                     /locus_tag="OQL90_000071"
     CDS             68655..69134
                     /locus_tag="OQL90_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCS1366985.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            69151..69456
                     /locus_tag="OQL90_000072"
     CDS             69151..69456
                     /locus_tag="OQL90_000072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1366986.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(69532..69996)
                     /locus_tag="OQL90_000073"
     CDS             complement(69532..69996)
                     /locus_tag="OQL90_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1366987.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(69993..70868)
                     /gene="nanK"
                     /locus_tag="OQL90_000074"
     CDS             complement(69993..70868)
                     /gene="nanK"
                     /locus_tag="OQL90_000074"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1366988.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(70865..71554)
                     /locus_tag="OQL90_000075"
     CDS             complement(70865..71554)
                     /locus_tag="OQL90_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1366989.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71602..73092)
                     /gene="nanT"
                     /locus_tag="OQL90_000076"
     CDS             complement(71602..73092)
                     /gene="nanT"
                     /locus_tag="OQL90_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1366990.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73201..74094)
                     /gene="nanA"
                     /locus_tag="OQL90_000077"
     CDS             complement(73201..74094)
                     /gene="nanA"
                     /locus_tag="OQL90_000077"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1366991.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74216..75007)
                     /gene="nanR"
                     /locus_tag="OQL90_000078"
     CDS             complement(74216..75007)
                     /gene="nanR"
                     /locus_tag="OQL90_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1366992.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            75387..76753
                     /gene="dcuC"
                     /locus_tag="OQL90_000079"
                     /pseudo
     CDS             75387..76753
                     /gene="dcuC"
                     /locus_tag="OQL90_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(76796..77293)
                     /gene="sspB"
                     /locus_tag="OQL90_000080"
     CDS             complement(76796..77293)
                     /gene="sspB"
                     /locus_tag="OQL90_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1366993.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77299..77937)
                     /gene="sspA"
                     /locus_tag="OQL90_000081"
     CDS             complement(77299..77937)
                     /gene="sspA"
                     /locus_tag="OQL90_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1366994.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78332..78724)
                     /gene="rpsI"
                     /locus_tag="OQL90_000082"
     CDS             complement(78332..78724)
                     /gene="rpsI"
                     /locus_tag="OQL90_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1366995.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78740..79168)
                     /gene="rplM"
                     /locus_tag="OQL90_000083"
     CDS             complement(78740..79168)
                     /gene="rplM"
                     /locus_tag="OQL90_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1366996.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79386..80513)
                     /gene="zapE"
                     /locus_tag="OQL90_000084"
     CDS             complement(79386..80513)
                     /gene="zapE"
                     /locus_tag="OQL90_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1366997.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80707..81105
                     /locus_tag="OQL90_000085"
     CDS             80707..81105
                     /locus_tag="OQL90_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1366998.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81259..82626
                     /gene="degQ"
                     /locus_tag="OQL90_000086"
     CDS             81259..82626
                     /gene="degQ"
                     /locus_tag="OQL90_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCS1366999.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            82716..83783
                     /gene="degS"
                     /locus_tag="OQL90_000087"
     CDS             82716..83783
                     /gene="degS"
                     /locus_tag="OQL90_000087"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1367000.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(83846..84784)
                     /gene="mdh"
                     /locus_tag="OQL90_000088"
     CDS             complement(83846..84784)
                     /gene="mdh"
                     /locus_tag="OQL90_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1367001.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPSEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85219..85689
                     /gene="argR"
                     /locus_tag="OQL90_000089"
     CDS             85219..85689
                     /gene="argR"
                     /locus_tag="OQL90_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1367002.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            86054..86317
                     /gene="yhcN"
                     /locus_tag="OQL90_000090"
     CDS             86054..86317
                     /gene="yhcN"
                     /locus_tag="OQL90_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1367003.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(86372..86644)
                     /locus_tag="OQL90_000091"
     CDS             complement(86372..86644)
                     /locus_tag="OQL90_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1367004.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(86736..88703)
                     /gene="aaeB"
                     /locus_tag="OQL90_000092"
     CDS             complement(86736..88703)
                     /gene="aaeB"
                     /locus_tag="OQL90_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1367005.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTERVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(88709..>89254)
                     /gene="aaeA"
                     /locus_tag="OQL90_000093"
     CDS             complement(88709..>89254)
                     /gene="aaeA"
                     /locus_tag="OQL90_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS1367006.1"
                     /translation="VLQTVLHQLAKAQATRDLAKLDLERTVIRAPADGWVTNLNVYTG
                     EFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSNKVLKGTVDSVAA
                     GVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPAGTTATVVVTGKQ
                     DRDESQDSFFRKMAHRLREFG"
BASE COUNT        21926 a        23987 c        22174 g        21167 t
ORIGIN      
        1 atgacagcct ggatattaaa accacattac aggcaaacga cattccagga ctcacaaccg
       61 accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc gtatattctg
      121 ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc gttaatattg
      181 gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg atgaccattc
      241 cgaaaacgga tattgccgtc attaaaggcg tgtgggtcga tggaatggga agcccgtcta
      301 caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc gccccacagt
      361 cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc aatggtcaga
      421 agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt gatatcactt
      481 atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc gacggtacaa
      541 ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac aatgtgcccg
      601 atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct tttcagaacg
      661 gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta
      721 atctggtcgg tggtgagctg gaaacaggaa aatttcaggg cacagccacc attaccgtca
      781 tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca ctgttacccc
      841 tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat ttctgcgacc
      901 agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt gcgcagggca
      961 tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac catttcgcct
     1021 ttgcgacggt tttcatcttc ctttacccac gccagcagtt cgccaacggg cgcgccgtga
     1081 atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga ttcgcctaat
     1141 accgcaacga tatcttccag gctatctaac agacggtggg tggattcata aaaaatcagc
     1201 gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt
     1261 aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa cgcagtgata
     1321 gcagcacacg gcccgggtag cggcaccacg cgaatccccg cttcacggca ggtacgcacc
     1381 agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttt
     1441 tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc
     1501 agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgagta
     1561 tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg ggtgatatcc
     1621 gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat
     1681 tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa
     1741 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     1801 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     1861 ccactgctta tatgcagggc acagcgcagg ctgattctgc cttttatctt cagcagatgc
     1921 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     1981 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     2041 attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg cagaaagatt
     2101 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     2161 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     2221 tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag cagaatattg
     2281 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     2341 acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc
     2401 gtaacgatcc cgatatgatg aaagccggga tcgccgactg gcagaaacgt tatccgaaca
     2461 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     2521 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     2581 ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg gcggctcagg
     2641 tagctgccgc agacgtagca gaacaacctc agccgcaaac tgcggatggc gttgccagcc
     2701 cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag ccggtgcctg
     2761 taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca tccgcagagc
     2821 tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag gttcagcagg
     2881 atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag ttgcttaaga
     2941 gcaacacccc gctgaacgtg ctggtactga accagccgga gaatatcgaa aaccgcgtca
     3001 atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc
     3061 gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg
     3121 tagccaatgc gtttgcgcag gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa
     3181 aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt atcgctttaa
     3241 cgggtacccc gattactccc agagcgacaa ccgactccgg catgacgacc aacaatccaa
     3301 cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg
     3361 tgtacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc gccatgcgta
     3421 acggtagcca gagcggtgca atgctgtacg ccagctcccg cagcgcacaa ggcaccgcag
     3481 gcccggattt ccgtctggag atggaaggtt tgcagtacag cgaaatcccg atgctggcgg
     3541 gcggtaatct gccgttaatg cagcaggcac tcagcgcggt gaataacgat tattcactgg
     3601 ctcgcatgta tgcgatgggc gtcgatgcct ggtcgctggc aaatcatttc tcacaaatgc
     3661 gccaggttca gggttttgaa atcaacggta ataccggaag cctgacggca aacccggatt
     3721 gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta gtccccgcca
     3781 gttaaccacc aaacagaccg gcgatgcgtg ggaagtacaa gcgcgtcgct ggctggaagg
     3841 caaaggactg cgatttgtcg ccgctaacgt gaacgagcgt ggcggtgaga tcgatctgat
     3901 aatgcgtgaa ggctggacca ccgtttttgt cgaggtgcgt taccgccgct ctgcgcttta
     3961 tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac agactgcccg
     4021 cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt tcgatgtggt
     4081 agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc actcataatt
     4141 aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa agcattcaaa
     4201 ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg
     4261 ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact tccgctgcca
     4321 atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg cccagcttac
     4381 ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac gatcgcttac
     4441 atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat gtattgttag
     4501 ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc gccgttacgc
     4561 gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca ggtttgttag
     4621 ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt caggaaatgc
     4681 atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg
     4741 atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt tccgcgctgc
     4801 tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt accaaagccg
     4861 caactgaccc acgcagtgtc ggtacccagg tggacgatgg taccctggaa gtgcgcgtga
     4921 acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat gtcacggcgt
     4981 atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg gctcgcgcca
     5041 aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt cgtcagggcc
     5101 agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa gtgcgttcgc
     5161 agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg
     5221 aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca gatattgcca
     5281 gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa tagcagcccc
     5341 ttgtaatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat gcaaaccgta
     5401 ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aacaacagcg
     5461 ccagcccggc caccagacaa atcgccgcaa aaccccagcc gaggacaaac cccataaaca
     5521 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
     5581 atacctgttg gcgacgcatc cccgccgcga ccggagccgc acagcaggta cacatcatgc
     5641 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
     5701 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
     5761 ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc gcttgccacg
     5821 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gctgcagtaa
     5881 acgctttgcc gtagtaatgt tgccatttca cataccagag accggcaatg acaacgagaa
     5941 agaaaagcgc gggtttccac cactgaatgg gtgttgccgc ctgagatgaa gactgaccag
     6001 tcatagcatt ccctaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
     6061 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
     6121 cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
     6181 caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
     6241 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
     6301 agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
     6361 agtgggcatt ggcccccata gcactgacca ccagcatatg ctgcgcgccc agccgccgcc
     6421 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
     6481 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt tcggtgacct
     6541 gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
     6601 acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc aacatccgca
     6661 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
     6721 ctttacagaa ttgtctgcct tgcgctccac ggctcatgca ccagtcttaa tagaccatga
     6781 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
     6841 tgagtaagaa aattgccgtt ttaatcagtg atgaatttga ggattcagaa tttacttcac
     6901 ccgcagacga gttccgtaaa gccggacacg aagtaattac cattgaaaag caagcgggta
     6961 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
     7021 tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
     7081 gtggggacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt
     7141 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
     7201 tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgcggaa ttttacgatc
     7261 aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag
     7321 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt
     7381 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
     7441 ggggccgaca gcattctggc aaccggaaag cgatgattgt acgccttgcg cgcgaggtcg
     7501 ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg aattaacgct
     7561 accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc
     7621 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga
     7681 taaaaagcat tagcgcacca cagttcccct tcctgctgca cacaccaggt gacaacatgt
     7741 tgttttgcca accaacggct gatggtgatg agtgtttcca ttgctgttct cttttatact
     7801 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
     7861 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
     7921 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
     7981 ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg ttaagcaact
     8041 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag
     8101 cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg
     8161 gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgccc
     8221 ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg actgtacagc
     8281 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
     8341 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt
     8401 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
     8461 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
     8521 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca
     8581 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
     8641 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
     8701 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
     8761 catgcagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
     8821 ctggatcttg tttacgcgcc gcaatcatca gcagatcgct gacgtcggca ctaaaactca
     8881 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
     8941 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
     9001 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
     9061 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
     9121 gggaacgaag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
     9181 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
     9241 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
     9301 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
     9361 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
     9421 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
     9481 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
     9541 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgcgcc gtggatatgg
     9601 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gaatacgccg
     9661 ccgagcgtta tccgaatatt gagcgccacg tatcggtgca ggcttcggcg accaatgaag
     9721 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
     9781 tgtcgattca tcaggtgaaa cagctggcac gggtcacacc tgtaccactg gaagtgtttg
     9841 ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg
     9901 gtgagtcgcc taacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa
     9961 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg
    10021 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    10081 acgcgctgga agaaccaacc agtctcaata ccttggaact gctgccggag ttaatggcgg
    10141 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    10201 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa agcgcgtgga
    10261 tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg tatcaccgta
    10321 aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa
    10381 agagacgctg gaagattttt atcagcaggc cgccaccagc agcgccgacg tgatttatct
    10441 tggagaagcg gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc
    10501 aaaatcgctc gccggaagtg gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc
    10561 atcctctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc
    10621 cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca
    10681 cgcgctgaac tgctacaacg cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg
    10741 ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga
    10801 tgagctgggc attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct
    10861 ggcctactcc gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga
    10921 aacctgctgc attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt
    10981 gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct
    11041 gacatccatg cagggattgg ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt
    11101 cgcgatgctc gacgccttcc gcgctaatga aaatggcgct gcgccactat tgttgacggc
    11161 aaacagcgac tgtaacggct actggcggcg gctggcagga ctggagctgc aagcctaagt
    11221 aaatatgctc gtcatacttc aagttgcatg tgctgcatct gcattcgttc accccagtca
    11281 cttacttatg taagctcctg gggattcact ctcttgtcgc cttcctgcaa ctcgaattat
    11341 ttagagcaca gctcactttg ttaacaactt taactactct ttaatgcagt attaaagatt
    11401 aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg ttactcgatc
    11461 tggcccccat tcccgaaggt tcttcagcgc gagaagcatt ctcccactct ctcgatctcg
    11521 cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga
    11581 ctggcattgc cagtgctgcc acgtcggtat tgattggcta tctggcggcg aataccacca
    11641 cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg
    11701 aacagttcgg cacgctcaat acactctatc cggggcgaat cgatttgggg ctgggtcgtg
    11761 cgccaggtag tgaccaacgg acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg
    11821 ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc
    11881 cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca
    11941 gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact
    12001 tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcgg
    12061 cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc
    12121 gcgatgccga attcctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgcggcg
    12181 aaaccgggca actgccgcca ccgattcaaa atatggatca gttctggtcg ccgtccgaac
    12241 agtatggtgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg aaagtgcgtc
    12301 atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtc aacgggcaga
    12361 ttttcgacca ccaggcgcgg ctgcattcgt ttgagctggc gatggatgtt aaggaagagt
    12421 tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg
    12481 tagacctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc
    12541 accgctgatt actgatacac cggcagtaaa ttaaagctcg ataaaatatg taccaatgcg
    12601 ttgccgacgc caaacaccag aatcagcgta atcatcggct tgccgcccca gacgcggaat
    12661 ttcgggctgc caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc
    12721 cagatggtag ccgctaaacc agcataacca atggcgtaca ggaatccatt cgggaacagc
    12781 agaccaccca caacaggcgg tgcaaaggtc aacaatgctg ttttcaagcg gcccatagcc
    12841 gagtcatcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg
    12901 aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg
    12961 ttcagcacgc cgcttaatgc ctgtaccagc acatcaatat taccgccctt ctctgcaata
    13021 ccgataaact ccggacgcgg gatgttaccc atcgtcgcca gcaaccagat ggtatacagc
    13081 gccagtgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg
    13141 taatacttca tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc
    13201 aatgtcatca gcagatacgg cgcataagat gcattgcttt ctgcaacgtt gaacaatgtc
    13261 gcaggctgca catgccccaa caggctgcca aaggtgagga agaaggtaat gactttcgcc
    13321 cccagcacaa tcgccgtcat gcggctgacg gccttagtac tcaaccacac cacaaacgct
    13381 accagcagtg caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg
    13441 aaggtgtgat gcagaattga accactggca gaaatatagg cataggtcag gatatagagc
    13501 acaaaggcaa tggaaatacc gttgaccacg ttccagcctt tgcccagcaa atctttggtg
    13561 atggtgtcaa aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg
    13621 gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc
    13681 ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc
    13741 accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct
    13801 tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacgcga
    13861 gtacaaaaga cagaaaaaaa gccccgatgg taaaaatcgg ggctgtatat attattttac
    13921 agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta
    13981 gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg acgaccatca
    14041 ccacggccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgttcgcca
    14101 ccgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt
    14161 aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca
    14221 cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttacca
    14281 atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg acgaacttca
    14341 acaccatcat cacggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt
    14401 tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg atcgcgcgga
    14461 ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggtacg
    14521 atcagagtac gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc
    14581 agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga
    14641 tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc
    14701 agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg
    14761 ttgcgcagca gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc
    14821 gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag
    14881 ttaactacca ggctgatacg ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc
    14941 aggatgtcca gacgaccatc tttcaggcgt tccagtgtct gttcacgcag cgcctggttc
    15001 atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagagcttc agccacttcc
    15061 agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg
    15121 aaacgtacca gtgcttcgtt tttgcgcata ccccagacag tccagtagct ctggctgatg
    15181 tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt cataaagcgg
    15241 cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct
    15301 tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg
    15361 tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg
    15421 tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt
    15481 aattgcacgt cataacgctg gccgccgtac agagcaacca catttacgcc gcgcatgtgt
    15541 ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc
    15601 accagaatct gtggtgcttt cagctcagga tcaagattct gcaacagagg taaagagaat
    15661 gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga
    15721 tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct
    15781 tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag
    15841 tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc
    15901 aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc
    15961 tcacccctta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt
    16021 gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg
    16081 cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg
    16141 tggcgctgtc cgaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac
    16201 tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg
    16261 tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat
    16321 ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agcttctgct
    16381 cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca
    16441 gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc ccgcgattca
    16501 agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag
    16561 catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaacact tcaggcatat
    16621 ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac
    16681 tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc
    16741 gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca
    16801 atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa
    16861 gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac
    16921 ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc
    16981 ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg
    17041 gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct
    17101 ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca
    17161 ctttctcaac gcgtttgtca gcgatttgag agatgtggac cagaccttct ttaccgccgc
    17221 cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc
    17281 ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc
    17341 cgtcggtcgc tgcgatcttc acagtaccgt catcttcgat ttcgatggta gtgccagttt
    17401 cttcggtcag ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg
    17461 ggttgatctt gatggtatgg atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt
    17521 tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg
    17581 caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga
    17641 taccctcgcg ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca
    17701 aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg
    17761 caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg
    17821 aagccataga agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg
    17881 ggaatttgtc catatccggc atgactgcca gcacgctgcg cttcgccaga cgaccgtgac
    17941 caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga
    18001 agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg
    18061 cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca
    18121 gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat
    18181 ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt
    18241 tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa
    18301 gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt
    18361 cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca
    18421 gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca
    18481 gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca
    18541 gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagaaca gcggcttcag
    18601 taccggcaac aaccagatcc agtttgctct ctttcagctc gtcctgagtc gggttcagta
    18661 cgtactggtc attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac
    18721 cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg
    18781 ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca
    18841 gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttggacgac
    18901 cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt
    18961 taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa
    19021 caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca
    19081 tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa
    19141 gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg
    19201 atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc
    19261 tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt
    19321 tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa
    19381 ctcggcagga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
    19441 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
    19501 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
    19561 gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac
    19621 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
    19681 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
    19741 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacttgt tatcgcaaga
    19801 cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcgacgc ggccttcatc
    19861 gtcaatttcg cccataccga taaacttgcc gttctcacct tctgtaacgc gaaccagtcc
    19921 ttccagcggc gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt
    19981 taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg
    20041 atcaagtaac tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag
    20101 atgttccagg gtcaccatcc gttcaaccgg atatttactt accgccagac gacgcaggta
    20161 aataacatgc gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata
    20221 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag
    20281 caattcataa atggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata
    20341 ttcgtacagt tttttaccct gatatttcag tgctgaatac atcgaaggga tctgttcgat
    20401 atcgccacgg aaagtatcca gcgccgccgc cagttgctct gcgctaaagg ttaccggacg
    20461 ttcttcaacg atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat
    20521 gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgaa
    20581 gcaaattggc aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt
    20641 ggcgttataa atccgtttca ctttttgcag cgcatcattg ctggacatac cctgcggttt
    20701 atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    20761 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    20821 aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc
    20881 acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc
    20941 aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat
    21001 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    21061 aggcgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    21121 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    21181 actgttagcc cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata
    21241 agacgcgcca gcgtcacatc aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg
    21301 catggaaccc taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc
    21361 gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc
    21421 gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta
    21481 gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt
    21541 aaccatacag cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc
    21601 cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac
    21661 ttcgtcaatc aggttataga tgacggagta gtaacgcaga tctaggcttt ccgcttcaat
    21721 cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc
    21781 cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac
    21841 tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcca cagaaccctg
    21901 tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa
    21961 catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg
    22021 acgatacagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc
    22081 cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag
    22141 cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag
    22201 aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg
    22261 acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt
    22321 cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga tgccggtacc
    22381 cgctttcgca gatacgtgta cgaactggct ttcaccgccc cactcttctg gcaggatgcc
    22441 atactgggag agttcgttct taacacgatc cggatcggct tctggtttat caatcttgtt
    22501 cactgcaacc accaccggta cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg
    22561 cggcatcacg ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc
    22621 acgagcacgc attgaagtaa acgcggcgtg ccctggggtg tccaggaagg tgatcatgcc
    22681 gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga
    22741 ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc
    22801 catgatggtc acaaccggcg cgcgcggttc agccgcagcc cccgtgtcac ggtcgctcat
    22861 taccgcctct tccagctcgt tttcacgacg caggataact ttgtggccca tctcttcagc
    22921 aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt
    22981 catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac
    23041 ggtgatggtt tcgccgatca caacgtcacg gttaacggcc tgagctggct tctggaagcc
    23101 ttgctgcagg gaagaacctt tacgttttcc gcctttacct ccacgtactg ctgcgcgagc
    23161 ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt
    23221 cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg
    23281 gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt
    23341 atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc
    23401 ttcttcgagt ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg
    23461 ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac
    23521 tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc
    23581 tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg
    23641 acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc gcttcacgct gcgcttgctc
    23701 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    23761 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    23821 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    23881 caaagtctgt ttctcttgtg cggacacaga gtcgtcagca gacttccgga tacctgcatc
    23941 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    24001 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    24061 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca ccccttcgat
    24121 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    24181 ggccagtttg aatgccaaat cacgatctat cccttcaagg ttcagcagat cgtcagccgg
    24241 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    24301 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    24361 catcggcaca taggccagtt cttccagcgt cgagaagcct tcttctacca gaacagtcgc
    24421 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    24481 ctgatgctta gcctgcaggt cgtcaacggt catcacgttg agttcccaac cgctcagctg
    24541 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccggcttc
    24601 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    24661 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    24721 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacacaggc
    24781 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    24841 atcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttctggca cttcaatacg
    24901 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    24961 ttccgggcga acggaataga gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    25021 cggcagcata tcttcgcgca ggatcacggc ttcagcattg ttgcccagct ccagagagat
    25081 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    25141 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    25201 cgtctgggta gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    25261 caggttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    25321 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    25381 gatctgtacg cggacgtcga tctcttgttc atattttttc tttgttgctg tcgccagcgc
    25441 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    25501 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    25561 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    25621 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    25681 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    25741 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    25801 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    25861 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgtgaccgcg
    25921 aataaattcg atgccaacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    25981 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    26041 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    26101 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    26161 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    26221 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    26281 ccaccccgcg cctgaaactc ggcaaattct actcgttttg ggtaaaagat gcaaatactg
    26341 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    26401 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    26461 tgaatacttt tgccttctcc tgctctccct taagcgcatt attttacaaa aaacacacta
    26521 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    26581 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    26641 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttctggcggt
    26701 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    26761 actgcaaacc taggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    26821 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    26881 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    26941 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    27001 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    27061 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    27121 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    27181 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    27241 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    27301 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    27361 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcaacgacgt agaaatgatg
    27421 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    27481 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    27541 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    27601 gcgcatggtc gtcagttggg ccgtctactg taccaggggc gttggtttga ctcccaggcg
    27661 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    27721 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    27781 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    27841 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    27901 tatgccaaaa ctggcctgct ctcctcctct gccacttcag gcgtgccgca ggtggagaat
    27961 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcagggggg ggttaccttg
    28021 cttatcctct gtaaactcct ttctgaacat cacataatcg aaaacatcat ggtttgcatc
    28081 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    28141 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    28201 gttgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    28261 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    28321 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    28381 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    28441 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    28501 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    28561 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    28621 catggcgcgc atggcgatac tggtaactgc agtaccattc tgccgaaaag cggattgcga
    28681 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    28741 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    28801 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    28861 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    28921 ctcccccaca atcaacacgt aggtatcaat acctgtatcc ctgactgata attgaaaata
    28981 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    29041 gttgagattg aaaaacggcg tataggtagc aaatcgcgac gctagtatat atggactgaa
    29101 cgttttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    29161 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    29221 tattattact gcccaaaaaa gaagggataa aaaagcaaag gcacatagat aagggatata
    29281 catccccagc attttgacaa cttcatccgg atcactctgg agcacactaa tcgcaaatcc
    29341 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    29401 agtcagtaac acaaatggtg cggcaacaag cagacgaagc ggaagggatg atatacgttt
    29461 tacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg cgccggaaga
    29521 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    29581 aaaacttcta gcaaatttgt tgaatactgt catccagaaa tccaagttca caaccaatat
    29641 aaacaattat caacaatgtt caattttata tcagacatgt ttttcaactc tgatccatat
    29701 cagaccgaaa cactgttagt tttttctttt taaatgatgc ggccattgcg ttctcagtaa
    29761 ttaaataaca ttatcaatgc gtctctaaac attacgcaga acgtagccat cagtagttat
    29821 actgacagga gcaggatggt ttatcgagaa gtaaacatat ccgtattcgt accggaactg
    29881 gagttgacac aataaagtgc caattatgtc agcagaaggg aaaagtttat aactaaggcg
    29941 taccggcacc atcgtttcaa ggtaccagct acgagtaaag caactggacg agatacagat
    30001 acctgacaac cattcctcag accaggacca aaacgaaaaa agacgctttt cagcgtctct
    30061 tttctggagt ttggtaccga ggacgggact tgaacccgta agccctattg ggcactacca
    30121 cctcaaggta gcgtgtctac caattccacc acctcggcac ggatactact ttttagttcg
    30181 ggatatcgct ggtcggctta gccggagcag ctggttgagt ttgttcggtt ttcgccggtg
    30241 cactcagatt ttcccattcg ctacctttat tggttttgtt gctgttgatg ttacccagca
    30301 ccagactgat gatgaagaat aacgttgcca gcagcgccgt catgcgggtc atgaagttac
    30361 cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc tccgaaggag gctcccatat
    30421 cagcgccttt accttgctgc agcatgatca gaccaacaag gccaattgcc acaataagga
    30481 aaactaccaa aagagcttcg tacataatca acctgttcct tgcggagttg ccgcgtacca
    30541 atgcttcaac caataaagcg ggagttttta atctttccca ctgaagcggg tgtgaatact
    30601 aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca ttgtatcaac tgcggaaaaa
    30661 aacagcaaaa ccgatgtatt cgctgagaaa aaaggcgaca atcgccgcct ttttagccag
    30721 tcatttatcg ttttaaacgg cttttactgc atcggcgatg cggtgtgcaa attcagtcac
    30781 ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac ggttcggtgc cggatttacg
    30841 cagcaacacg cgtccacggt tgcccagcgc agcttcaacc tctgcggtca cggctttaac
    30901 tgactcatgc tcaagcggat cgccgctacc tgcggtgtaa cgcacgttaa ccagaatctg
    30961 cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc atatggttac gtgccatcgc
    31021 cgccagcacc tgcaagccag caacgatgcc gtcaccggta gtggttttat ccagcaggat
    31081 cacatgaccg gaattctctg caccgatacg ccagcctttc tcctgcattt tttccagtac
    31141 gtagcggtca cccactttcg cgcgcgcaaa tggaatacct aactgtttca gcgccagctc
    31201 aagccccatg ttactcatca atgtacccac agcaccacca cgcagctggc cctgacgaag
    31261 accttcacgc gcgatgatat acatgatctg atcaccatcg actttattgc cttcatggtc
    31321 aaccataatc acgcgatcgc cgtcgccgtc gaaggcaata ccgagatccg ctttttcagc
    31381 cagcacacga gcctggagcg cgcgaacgtc ggtagccccc acttcggcat tgatgtttac
    31441 accgttcggc tcacaaccga tagcgataac gttcgccccc agttcacgca gcacgttcgg
    31501 cgcgatgtga taagtcgcac cgtttgcgca atccaccaca atcttcagtt cgctgaggct
    31561 aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag cgacccgcag catcaacgat
    31621 acggctggct ttacccagtt ctgccgaatc aacacagctg atctcttttt ccatttccgc
    31681 ttcgatggcc tcttctaccg catccggtag tttggtgccg tcgatagaga aaaatttaat
    31741 gccattatcg tagaacgggt tatgcgatgc agatatcaca attccggcct ctgcgcggaa
    31801 ggtacgcgtc agataagcca cggccggtgt tggcatcggg ccagtgaaga gtgcggaaag
    31861 gcccgctgcc gccagacccg cttccagtgc tgactccagc atatagccag aaatacgcgt
    31921 gtctttacca ataatgatct tacgggagcc gtggcgcgcc agcactttac ccgcggccca
    31981 acccagctta agcacaaaat caggtgtgat cggcgcatcc cctacacgac cacgaatccc
    32041 atcggtaccg aaatatttac gattactcat agcgtttgtt ttcctttgca gacagagtgg
    32101 cttccaccac ccgcatcgct tctacggttt ctttgacgtc atgaacacga ataatgtgcg
    32161 cgccttgcat tgcggcaatg accgcacagg ccagactacc gctcaggcgc tcggacggcc
    32221 ccacgttcag cagctgccca atcatcgatt ttcgtgacat acctaccaac agcggcaggt
    32281 tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata gttatgggag agatttttac
    32341 cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc gatacccgcc tgctcgcaac
    32401 gtgctatttg ctcaataaag tagcgattca cttctgcaaa gacatcgtca tacttcggag
    32461 cttcctgcat ggtttttgga tttccctgca tatgcatcag acaaaccggt aaaccggttt
    32521 ctgccgccgc ctccagagcg ccaggttcgg aaagggagcg gatatcgtta ataatgtgag
    32581 cgccaacttt cgctgactca cggatgactt ctggtttgga tgtatctacc gagagccaga
    32641 cttcgaagcg ttgagcaatt gcctcaacca caggaataac acgttgcaac tcttcttcaa
    32701 cgctaacttc cgccgcccct gggcgcgtgg actcgccacc aacgtcaatg atcgtcgcgc
    32761 cagcgttgat catcagattc gcatgtttca ccgcatctat cagcgagtta tgcgtgccac
    32821 catccgaaaa ggaatcaggc gtgacgttga ggatccccat tacgtgagga tggctaaggt
    32881 ccagtgaagt gccctgggca aagagtttca tggtgttatc cctggtatga attgataaga
    32941 aaaaaccccg gagcacgccc cggggttttc ggtacaaata cagtcatctg atgcgggaac
    33001 ttacttgtcg cctaactgct ctgacatggt gttacccggg ttcggcgtac gcggttcatc
    33061 aaccggacga ggagcctttg gactaccatt gtcgccagaa ttgttagaag cgcctggttc
    33121 ttcccagccc gctggcggac gtacatcgcg acgtgccatc aggtcatcaa tctgcggtgc
    33181 gtcgatagtc tcatatttca tgagagcatc tttcatcgca tgcagaatat ccatattgtc
    33241 ggtcagaagc tgacgcgcac gattatagtt acgctcaatc agtgctttca cttcctggtc
    33301 gatgatacgt gcagtttcat cggacatatg tttcgctttc gctacgctac ggccgaggaa
    33361 cacttcacct tcttcttccg cgtacagcag tggacccaat ttctcagaga agccccactg
    33421 agtcaccata ttacgcgcca ggttggtcgc aactttaata tcgttggacg caccggtaga
    33481 aacatgttcc ggcccgtaga tgatctcttc tgccagacga ccaccgtaca gcgtagaaat
    33541 ctggctttcc agtttctgac ggctggcgct gattgcgtcg ccctcaggca agaagaaagt
    33601 cacacccagc gcacgaccgc gtgggataat cgtcactttg tgcaccggat cgtgttccgg
    33661 caccaagcga ccgataatcg catgacccgc ttcgtggtaa gccgtcgatt ctttctgcgc
    33721 ttccgtcatc accatggagc gacgttccgc acccatcatg attttatctt tcgctttttc
    33781 gaactcaatc atcgacacaa cgcgtttgtt gccacgagca gcgaacagtg ccgcttcgtt
    33841 taccaggttc gccaggtcag caccggagaa accaggagta ccacgggcaa tgattgccgc
    33901 gtcgatatcg ggtgccaatg gtacgcgacg catgtgaact ttcaggatct gctcacgacc
    33961 gcgaacatct ggcaagccaa ccacaacctg acggtcgaaa cggccaggac gcagcagggc
    34021 tgggtcaaga acgtccggac ggttagtcgc ggcgataacg atgatacctt cgttaccttc
    34081 gaagccatcc atctcaacca gcatctggtt cagagtctgt tcacgttcat cgtgaccacc
    34141 gcccagacca gcgccacgct ggcggcctac ggcgtcgatt tcatcgataa agatgatgca
    34201 cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca cgggatgcac ccacaccgac
    34261 gaacatttct acgaagtcag aaccggagat agtaaagaac ggaactttcg cttcgcctgc
    34321 aatcgctttc gccagcaacg ttttaccggt acccggagga ccgaccatca ggacgccttt
    34381 cgggatctta ccgccgagtt tctggaagcg gctcggctcg cgcagatact ctaccagttc
    34441 agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca gcaaaggtcg ttttgatctg
    34501 atcttccgtc agcatgcgcg ctttgctctt accaaacgac atggcacctt tgccaccgcc
    34561 gccctgcatt tgacgcatga agaagatcca gacaccaatc agcaacagca tcgggaacca
    34621 agagatgaag atagaagcca gcaggcttgg ttcttcaggc ggttcaccga caaccttgac
    34681 gttcttggtc aacaggttat ccagtaattt cggatcctga accggaatgt aagtggtata
    34741 acggttacta tctttcttgg taacgttgat ttcacgtccg ttgatacgcg cttcacgaac
    34801 ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc accttacggc cattagactc
    34861 gctgggccca aagctctgga atactgacat cagcacaacg gcaatgacca gccagagtat
    34921 taggtttttc gccatgtcac tcaagggatt aacctcttat tacaactgtg ttaaaaacag
    34981 cgtcaggata ctctatatcc agcatctttc aaactttcgt ctgaaatctc ccggttaggg
    35041 tttacgcccg gtcgctacaa tatacacttc ccgcgaacgt gcacgagaag agtccggctt
    35101 acgaactttg accttcgtaa acagggagcg aatttccctt agatactcat cgaaaccttc
    35161 gccctggaac accttcacta caaaactgcc acctggcgct aatacatcac gacacatttc
    35221 taacgccagt tccaccagat acatggcacg ggggatatcc accgccggtg ttccgctcat
    35281 gtttggtgcc atatcggaca tgacaacctg gactttgctg tcgccaacgc gctccagcag
    35341 tgctttcatc accagttcat cacgaaaatc gccctgaaga aagtccacac caacgatagg
    35401 atccataggt aaaagatcgc aagcgatgat gcggcctttg ccgccaattt gggtgaccac
    35461 atattgtgac caaccacccg gagcagcacc aaggtcgaca accgtcattc ccggtttaaa
    35521 gagtttgtca ctttgctgta tttcatcaag tttaaaccag gcacgggaac gtaacccctt
    35581 tttctgtgcc tgttgaacat atttatcgct aaagtgttcc tgaagccagc ggctggagct
    35641 ggcagaacgc ttcttacctg tcatttaact ttcccatggg gataactcat cgtaaccaat
    35701 tgcgtaaatt tttacgcgcc tatttggtga tatatgggag atggcggtag aatgacccgt
    35761 tttcaatccc aacgtaagca aaaaatacga tgaatctgag tactaaacaa aaacagcacc
    35821 tgaaaggtct ggcacatccg ctcaagccag ttgttctgct tggcagtaat ggtttgaccg
    35881 aaggggtgct ggccgagatt gaacaagcgt tagagcacca tgaactcatc aaggtgaaaa
    35941 tcgccaccga agatcgcgaa acaaaaacct tgatcgtgga agctatcgtg cgcgaaaccg
    36001 gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct ttatcgccca actaaagaac
    36061 gtaaaatctc gctgccacgc taagattatc ctaaagttac acacatttgc tgtgtaaaac
    36121 gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc tgttcgttga taaaaggccg
    36181 catagcggcc tttttccttt ctttacaata catcaacatc ttgatgtatt gggtaattct
    36241 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    36301 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    36361 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    36421 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    36481 ttggtgacat caatcacctg cgcgttcgac agcttggctt cgatgtcttt aatacggcct
    36541 tcgcagaaac cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    36601 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    36661 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    36721 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    36781 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    36841 ggtcagctac aattcacatt ttgatagtca ttttaccctg aagttcccga agggtcatcg
    36901 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    36961 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    37021 gccgcgaatg ttgatgagta cgttactcaa ctccccgctg gtgccaacct tgccctgatg
    37081 gtgcaaaaag tcggcgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    37141 cccgccagta cgcagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    37201 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    37261 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    37321 atcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    37381 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    37441 tttagcgctc cgcctgccgc tgccatagtt gaccgcaact gtttctccgt ctcgctctac
    37501 agtgccccaa aacctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    37561 atgttcagcc aggtacgcac cctcccccga ggttctgccg aagcgcaata ctgcgaactg
    37621 gatgtcatac caggcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    37681 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg ggcaattctg
    37741 aaagatgagt taaaacaggc aggtatcacc tggagcggaa cactgctgcg ccagactcag
    37801 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgttgca cgatctgctt
    37861 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgataggc
    37921 catgcgcgct tcaatgtgcc tggaacatgg cgggccgggt cggacgccgt gcgtcagatc
    37981 ctgcgccagc aagccggtgt cgatattgga aacaccatta ttgccgatgg ttcagggctt
    38041 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgttgcaata cattgcccaa
    38101 cacgacaatg aacttaactt tatctccatg ctgccgctgg cgggctatga tggctctttg
    38161 cagtaccgtg caggtctgca tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt
    38221 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    38281 gcgtttgtac aatatctttc tggctatgca gtagaaccgg cggatcagcg taatcgccgt
    38341 attccgttag tgcgttttga aagccgtttg tataaagata tttatcagaa caattagtca
    38401 aaaagaaacc ccggcacatg gccggggttt tgctgattaa cgcttgtaaa tgaactcaac
    38461 gccttcttcg tcgtcttcgt cccagtcatc atcccagtcc tcatcatcct cttcagcaat
    38521 ctcttcaagc tgctggcgat gataatcatc ccacatgaat tcgactttct ctggctgttt
    38581 cgcttcttca gcctgcacga ccgggttttc aatgataaag gtcatcacat cccagcagag
    38641 atctttcacg cccagtccac tcgccgcaga gatcagataa tatttatctt cccagcccag
    38701 cgcttcagca atcgctttcg ctttctcttc agcttctgcc ttatccagca gatcgatctt
    38761 attgaacact aaccaacgcg gtttcgccgc cagatcctgg ctgtattttt ccagctcgct
    38821 gataataata cgcgcgtttt caaccggatc ggtgccgtca atcggatcga tatcgatgag
    38881 gtgcaacagg acgcggcaac gttccaggtg cttcaggaag cggataccca gacctgcgcc
    38941 ttccgcagcg ccttcaatca gtcctggaat atcggcaaca acgaagctct tttcgttgtc
    39001 cattcgtacc acacccagac ttggcaccag agtggtaaac ggataatccg ccactttcgg
    39061 tttagccgcc gataccgcgc gaataaaggt cgatttaccc gcgtttggca tccccaacat
    39121 accgacgtca gccagcagca tcagctccag cagcagctca cgcttatcac ccggcgtacc
    39181 gttggttttc tgccgtggtg tacggttaac ggacgatttg aaacgggtat tgcccagacc
    39241 gtgccagccg cccttagcaa ccagcagacg ctgaccgtgt ttagtcatat cgcccatggt
    39301 ttcaccagta ccctggtcga ttacacgcgt acctaccggc actttaatcg tcacgtcttt
    39361 accgcgctta ccggtacaat cgcggcttgc gccgttctga ccgcgctctg cacggaaaga
    39421 tttttcaaaa cgataatcga taagcgtgtt caggttttcg tcggcttcca tccatacgtc
    39481 accaccatca ccaccgtcgc cgccatccgg gccgcctttc ggaatatact tttcgcggcg
    39541 gaagctcacg caaccattac cgccatcacc tgcaacgacc agaatcgatg cttcatcaac
    39601 aaacttcatt ttattctccg taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt
    39661 atgcttacgt aatccgcccc aaatacgatg accaatggcg gaatacatcg cgcccgcaac
    39721 cacgacaaac gcaccgagat aacctaaaag gtttaacatc ggtctggcga agaaatcggg
    39781 ccaggccagt gataaaagat ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat
    39841 cgcgctcacc tgcgctgcct gccagcgagc catcgcttcc gccagggcgc catatcctac
    39901 caatgtattc agtccgcaaa aaattaaaca tgcgagctgc cagtggctaa gctgcgctat
    39961 cactcctggc tttgccagag ggaagagcgc aattgtacat aaagtgtaca gcaaaaacag
    40021 gatctgcggt gaggccagcc gacgcaataa aaccttttgc gccacgccat aactcaccca
    40081 aaccgtcgcc gcaccgaccc caaagatgac tccccaggtg taatcagtga gctttgtaaa
    40141 tatctcgacc agactggtgt taaaaaacat caccaggccg ctcaggagca tcaatgcccc
    40201 tacaacctga gtgctgcgca ttttctcttt caggataaat acgctggcaa ccatcatgcc
    40261 aactggcgag agttgcccaa tcacctgcga agcggtcgga ctcaggtatt gcaaggatga
    40321 gctgaacagg atgaagttcc caaacagccc ggcggtcgcc actgccaaca aaatcaacca
    40381 gcgtggctta cgaaacacgc gtaatggcgg caacctcttc ttcaccgcaa gaatggcacc
    40441 caggccaata ctcgccatca agaaacggta aaacacgatt gtcggaggtt ccatcacctc
    40501 cagcacctgc ttcattgcga ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa
    40561 aagaatgcca atgcctgcct gctgcttcat accgtattcc ctgcaaagcg catttgcgga
    40621 ttaacgtcgc accatgctca tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt
    40681 tgcggggctt tcatccgtta ccgggacgcg aaaaacttat tcagcttcga tgctgataaa
    40741 tttacggttt ttcgggcctt taacttcgaa tttcacttta ccgtctgctt tagcaaacag
    40801 agtgtggtca cgaccgcaac ctacgttagc gccagcgtgg aatttggtac cacgttgacg
    40861 aacgatgatg ctacccgcca gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt
    40921 agcttctgaa tcgcgaccgt tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa
    40981 tctctcctca ggtcttaggc gctgatgcca gtaattttca catcagtgaa ccactgacga
    41041 tggccctgct gcttacgata gtgtttacga cgacgaaact taacgatttt aactttctcg
    41101 ccacgaccgt gagcaacaac ttcagctttg attacgccgc catcaacgaa aggaacgccg
    41161 attttgactt cttcaccgtt tgcgatcatc agcacttcag cgaactcaac agtttcgcca
    41221 gttgcgatgt ccagcttttc caggcgaacg gtctgacctt cgcttactcg gtgttgttta
    41281 ccaccacttt ggaaaaccgc gtacatataa aactccgctt ccgcgcacac cttttcaatg
    41341 attcagagtg cgctataaat attcacaata gggcgcgaat attacgcaaa acgcacgcct
    41401 ttgacaagtg ctacagtcaa tacacgaaga aaaaaaacac aacttgtacg gtaacgttta
    41461 tctgtgccat tttttcagta caatcaccct atattcctaa ccataaaccc taagttgcct
    41521 ttgttcacag taaggtaatc ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa
    41581 tcaatgagtt aaccgcgcaa gatatggcgg gtgttaatgc ggcaatcctt gagcagctta
    41641 attccgacgt ccaactgatc aatcagttag gctattacat cgtcagcggc ggcggtaaac
    41701 gtattcgtcc gatgattgct gtactggctg cacgagctgt tggctatgag ggaaatgcgc
    41761 atgtcaccat tgctgccctg atcgagttta tccacacggc gactctgcta cacgacgacg
    41821 ttgtggatga atcagatatg cgcaggggta aagctaccgc caacgctgca tttggcaatg
    41881 ccgccagcgt gctggtaggc gattttattt atacccgcgc gttccagatg atgaccagcc
    41941 tcggttcact caaagtgctg gaagtcatgt cagaagctgt aaacgtcatc gcagaaggtg
    42001 aagttctgca actgatgaac gttaacgatc cggacatcac tgaagaaaac tacatgcgcg
    42061 ttatctatag caaaaccgcg cgtctgtttg aggctgccgc gcagtgttcc gggattctgg
    42121 ctggctgtac gccggaggag gagaaaggcc tgcaggatta tgggcgctat ctcggcactg
    42181 ctttccagtt gatcgacgat ttactcgatt acaatgccga tggcgaacag ttaggtaaaa
    42241 acgtcggcga cgatctgaac gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc
    42301 atggcacacc agaacaggca cagatgatcc gtaccgccat cgaacagggt aacggtcgcc
    42361 atcttctgga accggttctg gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc
    42421 agcgcgccga ggaagaagca gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc
    42481 cttggcgaga agcactcatc ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc
    42541 ctcccttcat cccgcgcagt gcgcgggatg attccagtaa attccataaa aacactaatt
    42601 cagcgctata acacctgaaa aaaagggcga tgaatcttta tatattctga atattcacac
    42661 tctttacagg aactttttag agcaataggc catcaggagt atagtgatgc ccgacagaag
    42721 aagtgttctg aatgaaagcg actaacttaa ggagtgagga aagatggaaa gtaatttcat
    42781 tgactggcat ccccctgaca tcattgcagg tttgcgcaag aaaggaacgt caatggcggc
    42841 ggaatctcgc agaaatggtt tgagttcctc aacgctggca aacgcattat cgcgcccatg
    42901 gccgaaagga gagatgatta ttgcgaaagc cctgggaact gacccctggg ttatctggcc
    42961 atcacgctac catgatccgc agacccatga gtttatcgac agaacgcagt tgatgcgtag
    43021 ctacactaaa ccgaaaaaat gagtggtctg gcggtagccc cgcgaacggg gctgccagct
    43081 ctcagacgat tactcgcctt tcacacgctt aatatttgca cctaaagcgc gcagtttgtc
    43141 ttcaatgcgt tcgtagccac gatcgatgtg ataaatacga tcaaccaccg tcgtcccttc
    43201 cgcaatacag ccagccagca ccaggcttgc tgatgcacgc agatcggttg ccataacctg
    43261 tgcaccagaa agtttttcaa caccatgaca aataacggta ttgctttcga tttcggcatg
    43321 cgcgcccata cggctaagct ccggcacatg cataaagcgg ttttcaaaga ccgtttcggt
    43381 gatgaatccg gtcccttctg ccaccaggtt caacagcgtg aactgggcct gcatatcggt
    43441 cgggaatgcc ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt tgccatgcat
    43501 atccaggcta atccagtctt cgccgacttc gatgtccgct ccagcgtcac gcagtttcgc
    43561 cagcacggcg tcgagagtat ctggctgcgc gttacggcag ataattttgc cgcgagaaat
    43621 cgccgccgcc accaggaaag taccggtttc gatacgatcc ggtagcacgc gatagacacc
    43681 gccgcctaaa cgttccacac cttcgatgac gatacgatcg gtgccctgac cgctaatttt
    43741 cgcacccagc gtaatcagga agttcgcggt atcgacgatt tccggttcac gcgctgcgtt
    43801 ttcaataatc gtggtacctt ccgccagggt tgcagcacac atgatggtca ccgttgcgcc
    43861 aacgctgact ttatccatca cgatatgtgc gcctttcaga cgaccatcga cggaagcttt
    43921 aacgtaacct tcttccagtt tgatggtcgc gcctaattgt tcgaggccag aaatgtgtag
    43981 atcaaccgga cgcgcaccga tcgtacaacc gccaggcagt gaaacttgcc cctgaccaaa
    44041 gcgcgctacc agcggcccca gtgcccagat agaagcacgc atggttttaa ccagatcgta
    44101 aggtgcgcag aatacattaa cgtcgcgggc atcaatatgc acagaaccat tacgttctac
    44161 tttcgcaccc agctgactta gcagcttcat tgatgtatcg acgtctttca gtttcgggac
    44221 gttctggatc tctaccggtt cttccgccag tagagcggca aaaaggatag gcagagcagc
    44281 atttttagcg ccggaaattg tgacttcgcc ctggagcttc gttggcccct gaacacgaaa
    44341 tttatccatt tagtttgttc tcagttaaca attcatatcc gctaccggcg aatcgcccat
    44401 agctcaaaag ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat acgctttgat
    44461 cgacacagca tgaatgcggt tatccgcaat atattccatc agcgggccat agaccgtctg
    44521 ctgtttttta acccgactca tgccgtcaaa caactcaccc acggcaataa cctgaaagtg
    44581 gctgccatcg ccggaaacgt ggacttcctg gagggagaga gcgttcatca acacgctctg
    44641 aatttcatta ttttccatgg gatcttcaat catcagttaa taaaccagcg aaacatctta
    44701 gagcaaagtt gcgctggcat aaataagcaa aaagcctcgc tgataaatca gacaaggctc
    44761 gacttgcagg caggtttgcc ggacaggcgg ttaacgccat atccggcctg aaaaaattta
    44821 acgaggcaga acatcagcag gcaaattata caatttcgcc agggtataca ctttgtcgtt
    44881 taccccctga agcgtcacat tgttgccctg ctttttcgcc agatcgataa gatggagcag
    44941 cagtgccagt ccccccgtat ccacgcggga cacacggcta agatcgatgc aggtaatccc
    45001 cttcaccgct tcctcacgca tttcccaaag cggtagcaaa acgtcctgat ccagctctcc
    45061 ggataacgcc agcgtgtcac ccgtctgcat ccagctcagt gactcgctca ttattttttc
    45121 tcttccagag tgattttctg ttgagaaatc gatttcagtt gcgcagtcag gccgtcgata
    45181 cctttggtac gcagcagcgt tccccactcg ttttgtttgg tggtgatcat actgacgcct
    45241 tcagcaatca tgtcgtaagc ctgccagttg cccgtctggg agtttttacg ccactggaag
    45301 tccagacgca ccggcgggcg gccattcgga tcaataatgg taacgcgaat cggcacaatg
    45361 gttttatcgc ccagcggctg ttctggcgca atctgatagg tttgaccatg atacatcgcc
    45421 agcgcctgac cgtaagcctg cttcaggtac tcacggaaag cggcaaagta ggcttcacgt
    45481 tgagcagggg tcgcactctt gtaatactgg cccagcacca gcgcaccggc gtatttcacc
    45541 tgtacgtatg gcagcagttc ctgatcaaca atggtacgca gataatccgg gttggcccga
    45601 atttgcggtt gctcattctt caggcgatcg aacgttttct gcgccgcctc gtccatcagc
    45661 ttataaggat tggtctggtc tgccgcggtt gccgcactca gcggtgcaat caccagcaaa
    45721 gcgaccatca ttaaacgttt aaacatgcgt cggttctcct gaaattattt cgttgtaccc
    45781 acaggttcag tggtttcatt attacctggc gcagcagctg gcgcatcgcc actattctta
    45841 ttgtcatcgc ctttactacc gtaaaggaac tgaccaatga gatcttccag caccatcgca
    45901 gacttagtgt cctgaattgt atcgccatcc ttcaggatag cagtccccag ttccgggtct
    45961 tcaaaaccga cgtttaatgc cagatattgt tcccccagta ggccggaagt acgaatgctc
    46021 agcgaactgg tatctggaat gtggttataa cgttgttcaa tttccagcgt tacgcgtggc
    46081 agataggttt tcgggtccag cgtaatatcc gccacccggc ccacaacaac gccaccaata
    46141 ctgaccggag agcgcgcttt caggccgcca atgttatcga acgtcgcata aagcgtgtag
    46201 gtcggttcag tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa cagagccgcc
    46261 agcagtgctg ctaataaaaa gatacccacc caaatttcat tttttttcgt ttgcatgtac
    46321 tcaattccca aacatcaatg cggtcagcac aaaatccagc cccagaacag ccagagacga
    46381 gtggacaacg gtgcgagtgg ttgcccggct aatcccggca gacgtcggga tggcgtcgta
    46441 gccgttaaac aacgaaatcc acgtcaccgt gatggcgaac accacgctct taatcagaca
    46501 gttgaccaga tccatacgcc agtcgacggc attttgcatt gccgaccaga agaacccgct
    46561 atcaatgcct ttccaactga cgccgaccag agatccgccc cagatcccca cggcgacgaa
    46621 aataaccgtc aacagtggta atgaaataac cccagcccag aaacggggag aaataacccg
    46681 acgcagcgga tccaccgcca tcatctccat actggagagt tgctctgtag cgcgcatcag
    46741 gccgatttct gcggttagcg ccgaaccagc acgcccggca aacaacaacg cggcaaccac
    46801 cggccccagt tcacgcagta gcgataacgc caccagcata cccagactgg tttccgcact
    46861 ataagtggtc agaaccagat aaccttgcag ccccaacacc attccgatga acacgccaga
    46921 aaccacaata atcagcatcg acaggacgcc gacattatag agctggcgca ccagcagcgg
    46981 cgcatgtttg cgaaattccg gtttgccgac cagcgcattg aataacatta acccggcccg
    47041 cccgaacgtt ctcagggttt taatcccttt atgtccgagc gacgccagcg catttaacag
    47101 catgagtggc ttaactccct ggtaaaagat cagcgtgata atcgccggca ggatagcgga
    47161 acggaacagg cccgtcagct atcccgtcca gaaactgacg tacgcgcgga tcaggattcg
    47221 cctgcaacgc ctgggcacag ccatgagcga caattttttt gtccgccagg atccaggcgt
    47281 gatccgcaat acttaacact tccggcacat cgtgagaaac caccacacaa gtcacgccca
    47341 gcgcgctgtt cagctcagaa atcagcttca ccagtacgcc catggtgatg ggatcttgcc
    47401 caacaaaagg ttcatcaaac atgatgagat ccggctccag cgcaatcgca cgcgccagcg
    47461 ctgcacgccg cgccatccca ccggaaagtt cagaaggcat tagtttagcc gctccacgca
    47521 gccccacggc ctccagcttc atcatcaccg tactatgcaa caatggcgcg ggaagttggg
    47581 tatgttcgcg cagtggatag gcgacgttgt caaatacgtt catatcagtg aacaacgccc
    47641 cggactgaaa taacatgctc atccgtttgc gcactgtata caggcgcgaa cgagacatcg
    47701 ccggaatatt ctcaccatcg aaaaggatct caccatgatc tggtgcgatt tgcccgccaa
    47761 tcagacggag tagcgtcgtt ttaccgatgc ccgatggccc catgatcgcc gtgatcttcc
    47821 ctcgcggcac ggtcagggaa atattatcga agatgcagcg attgccacgc gtaaaactga
    47881 catcgcgcat atcgactaaa ttcgccacag actgctccat aattcaccct tcgtcttgcg
    47941 ttgattttct aagcatggcg ctcaatttaa ccttgaaccc aacatattta cagaatatta
    48001 cccgctgtgg ttagcgaaag ctggcatttg ttttactttt tagccgcata aagtcaaaat
    48061 taagaatccg ttacggcttt ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa
    48121 ggacgttaga tgcttttagc tacggcactg ttaattgttg gtttactttt ggtcgtttac
    48181 agtgccgacc gcctggtttt tgccgcgtct attctttgcc gaacctttgg cataccgccg
    48241 ctgatcatcg gcatgacggt ggtcagtatt ggtacatcgt taccagaaat catcgtctcg
    48301 cttgccgcgt ctctgcacga acaacgcgat ttagccgttg gtacagccct cggctcaaac
    48361 attatcaata ttttgctgat cctcggtctg gctgcgctgg ttcgtccttt taccgtccat
    48421 tccgatgttc tgcgtcgtga attaccctta atgttgttgg tcagcgtagt ggccggttcc
    48481 gtactctatg acggacaact tagtcgcagc gatggtatct ttctcctctt tctggctgtg
    48541 ctatggctgc tgttcattgt taaacttgca cgtcaggctg aacgtcaggg gactgacagc
    48601 ctgaccagag agcagcttgc agagctgccg cgtgacggcg gattgcccgt cgcgttttta
    48661 tggctcggca ttgcgcttat catcatgcca gtggccacgc ggatggtggt tgataacgcc
    48721 acggtgctgg cgaattactt tgccatcagc gagttgacga tgggtctgac ggcaattgct
    48781 atcggaacca gcctgccgga actggcaacc gcaatagcgg gggttcgcaa aggtgaaaac
    48841 gacattgctg tcggaaatat cattggcgca aacattttta atattgtcat cgtgttgggt
    48901 ttacccgcgc tgataacacc aggagagatt gatccattgg cgtacagtcg tgactacagc
    48961 gtgatgttgc tggtgagcat tatttttgcg ttgctgtgct ggcggcgctc cccgcaaccg
    49021 ggccgtggtg taggggtatt attaactggc ggatttatcg tatggctggc gatgttgtac
    49081 tggttatcgc caatactcgt tgaataactg gaaacgcatt atgtcgcacg tagagttaca
    49141 accgggtttt gactttcagc aagcaggtaa agaagtcctg gcgattgaac gtgaatgcct
    49201 ggcggagctt gatcaataca tcaatcagaa tttcacgctt gcctgtgaaa agatgttctg
    49261 gtgtaaaggg aaagttgtcg tcatggggat ggggaaatcg gggcacatcg ggcgcaaaat
    49321 ggccgcaacg tttgccagca ccggtacacc ttcatttttc gtccatcctg gtgaagccgc
    49381 gcatggtgat ttaggcatgg tcaccccaca ggatgtggtg attgctatct ctaactcagg
    49441 tgaatccagc gaaatcacgg ccttaattcc agtgcttaag cgtcttcacg taccgttaat
    49501 ctgcatcacc ggtcgcccgg agagcagcat ggcgcgcgcc gcagatgtgc atctgtgtgt
    49561 taaagtagcg aaagaagcct gtccgttagg gctggcaccg accagcagca ccaccgccac
    49621 gctggttatg ggcgatgccc tcgctgtcgc gctgttaaaa gcacgcggct ttactgctga
    49681 agattttgcg ctctcacacc caggcggcgc actgggtcgt aaacttctgc tgcgcgtaaa
    49741 cgatattatg catacgggcg atgagatccc gcatgttaag aaaacggcca gtctgcgtga
    49801 cgcattgctg gaagttaccc gcaaaaatct tggtatgact gtcatttgcg atgacaatat
    49861 gatgattgaa ggcatcttta ccgacggtga tttacgccgt gtcttcgata tgggagtgga
    49921 tgttcgtcag ttaagtattg ccgatgtgat gacgccgggg ggaatacgcg tgcgccctgg
    49981 cattctggcc gttgaggcac tgaacttaat gcagtcccgc catatcacct ccgtgatggt
    50041 tgccgatggc gaccatttac tcggtgtgtt acatatgcat gatttactgc gtgcaggcgt
    50101 agtgtaaaga ttcaaggata aacaacaatg agcaaagcag gtgcgtcgct tgcgacctgt
    50161 tacggccctg tcagcgccga cgttatagca aaagcagaga acattcgtct gctgatcctc
    50221 gacgttgatg gcgtactgtc agatggcctg atttatatgg gcaataacgg cgaagagctg
    50281 aaagcgttca atgttcgtga cggttatggc attcgttgtg cgctcacctc tgatattgaa
    50341 gtcgctatca ttaccgggcg aaaggctaaa ctggtagaag atcgttgtgc cacattgggg
    50401 atcactcact tgtatcaggg gcagtcaaac aaactgatcg cctttagcga actgctggaa
    50461 aaactggcga ttgccccgga aaatgtggct tatgtcggcg atgatctcat cgactggccg
    50521 gtaatggaaa aagtgggttt aagcgtcgcc gtggccgatg ctcatccact gttgatcccg
    50581 cgcgccgatt atgtgacgcg cattgctggc ggtcgtggcg cagtgcgaga agtttgcgac
    50641 ttattactcc tggcgcaggg caaactggat gaagccaaag ggcaatcgat atgagtaaag
    50701 ccagacgttg ggttaccatt gtgctatcac tggcggttct ggtgatgatc ggcattaata
    50761 tggccgaaaa agacgatacc gcccaggtgg tcgtcaacaa caatgatccc acctataaaa
    50821 gcgagcatac ggacacgctc gtctataacc cagaaggggc actaagctat cgattgattg
    50881 ctcaacacgt tgaatattat tccgatcagg ccgtttcgtg gtttacgcag ccggtactta
    50941 ccacgtttga taaggataaa atcccgacat ggtccgtaaa agcagataaa gccaagctga
    51001 ccaatgaccg gatgctctat ttatatggac acgttgaagt caacgcactt gtgccggact
    51061 ctcaacttcg cagaatcacg acggataacg cgcagatcaa tctggtgacg caggatgtta
    51121 cctctgaaga cctcgtcacg ttatacggaa caacatttaa ctccagcggt ctgaaaatgc
    51181 gcggcaactt acgcagcaag aacgccgagc tgattgaaaa ggttagaaca tcctatgaaa
    51241 ttcaaaacaa acaaactcag ccttaatctt gtgcttgcca gctcacttct ggccgccagc
    51301 attccggcat ttgccgtaac cggagacact gatcagccga tccacattga atcggaccag
    51361 caatctcttg atatgcaagg caacgtggtt acctttaccg gtaatgtcat cgtcacccag
    51421 ggcaccatca aaattaatgc cgacaaagtg gtcgttaccc gtccgggcgg cgaacaaggt
    51481 aaagaagtga ttgacggcta cggtaaaccg gcaacgttct accagatgca ggacaacggt
    51541 aaacccgttg aaggtcatgc ttcccagatg cactacgaac tggcaaaaga ttttgtcgtt
    51601 ctgacgggta atgcttatct gcagcaggtc gatagcaaca ttaagggcga taagatcact
    51661 tacctggtga aagagcagaa aatgcaggct ttcagcgaca aaggcaagcg cgtaacaacc
    51721 gttctggtgc cgtcgcagct gcaggacaaa aacaacaaag gccagacccc ggcacagaag
    51781 aagggtaatt aattcgttat ggcaacatta actgcaaaga accttgcaaa agcctataaa
    51841 ggccgtcgcg tggtagaaga cgtcagcctg accgtcaact ccggggaaat tgtcggtctg
    51901 ctggggccaa acggtgccgg taagaccacc actttctata tggttgttgg cattgtgccg
    51961 cgcgatgcgg gcaacatcat tattgatgat gacgatatca gtctgctgcc tctgcatgca
    52021 cgcgcgcgcc gcggtatcgg ctatctgcca caggaagcct ccattttccg tcgcctcagc
    52081 gtttacgata acctgatggc ggtactgcaa attcgtgacg acttgtctgc tgaacaacgt
    52141 gaagaccgcg cgaacgagct gatggaagag tttcacattg agcacctgcg tgacagcatg
    52201 gggcagtcac tctccggggg tgaacgtcgc cgtgtagaaa ttgcccgcgc actggctgcg
    52261 aatccgaaat ttattctact cgacgaaccg tttgccgggg ttgacccgat ctcggttatc
    52321 gacattaaac gcatcattga acacctgcgc gacagcggcc tgggcgtact gatcactgac
    52381 cacaacgtgc gtgaaacact ggcggtttgt gaacgcgctt atatcgtcag tcaggggcat
    52441 ttgattgccc acggcacgcc tacagaaatc ttacaagacg aacacgttaa gcgtgtatac
    52501 cttggggaag acttcagact ctgatagggt agaagtttgc gacgttttag caggagagta
    52561 cgattctgaa catgaagcaa ggtttgcaac tcaggcttag ccaacaactg gcgatgacgc
    52621 cacagcttca acaggcgatt cgtctgttgc agttgtcgac gctggaactt cagcaggagc
    52681 tacagcaggc gctggagagt aatccgctgc ttgagcaaat cgacactcat gaagaaatcg
    52741 acaccagcga aacgcaagac agtgaaacgc tggacaccgc cgacgcgctc gaacaaaaag
    52801 agatgccgga agagctgccg ctcgatgcca gttgggacac catttacacc gctggtacac
    52861 catccggcac cagcggtgac tacattgacg atgagctgcc ggtctatcag ggcgaaacga
    52921 cgcagacctt gcaggattac ctgatgtggc aggtcgagct gacaccgttt tccgacactg
    52981 accgcgctat tgctacctct atcgtcgatg ccgttgatga caccggttat ctgactgtcc
    53041 cgctggaaga tattctcgaa agtatgggcg atgaagagat cgacatcgac gaggttgaag
    53101 ccgtccttaa gcggatccaa cggtttgatc cggtcggtgt agcggcaaaa gatctgcgtg
    53161 actgcctgct aatccaactc tcccaattcg ataaaaccac gccgtggctg gaagaggcca
    53221 gactgatcat tagcgatcat ctcgatctgt tagccaatca cgacttccgc actttaatgc
    53281 gcgtcacgcg tctgaaagaa gatgtgctga aagaagccgt caatctgatc cagtcgctcg
    53341 atccgcgccc cgggcagtcg atccagactg gcgaacctga gtatgtcatt ccagatgtgc
    53401 tggtgcgtaa gcataacggt cactggacgg tagaactcaa cagtgacagc attccgcgtc
    53461 tgcaaatcaa ccagcactac gcctcgatgt gcaataacgc gcgcaacgat ggtgacagcc
    53521 agtttatccg cagcaatctg caggatgcca aatggttgat caagagtctg gaaagccgta
    53581 acgatacgct actgcgcgtg agtcgctgta tcgttgaaca gcagcaagcc ttctttgagc
    53641 aaggtgaaga atatatgaaa ccgatggtac tggccgatat cgcccaggct gtcgaaatgc
    53701 atgaatcgac gatatctcgc gtgaccacgc aaaaatacct gcatagtcca cgaggcattt
    53761 ttgaactgaa gtatttcttt tccagtcacg tcaataccga gggcggcggc gaagcttcct
    53821 ccacggcgat tcgtgcgctg gtgaagaaat taatcgcggc ggaaaaccca gcgaaaccgt
    53881 tgagcgacag caagttaacc tctttgctgt cggaacaagg tatcatggtg gcacgccgca
    53941 ctgttgcgaa gtaccgagag tctttatcca ttccgccgtc aaaccagcgt aaacagctcg
    54001 tttgacccaa ccgataagga agacactatg cagctcaaca ttaccggaaa taacgtcgag
    54061 atcaccgagg cactgcgcga atttgttaga gccaaatttg ccaaacttga gcaatatttt
    54121 gaccgaatca accaggtcta tgttgttctg aaagtggaga aagtcaccca cacctcagat
    54181 gcaacactgc atgtaaacgg tggtgaaatt catgccagcg cggaaggtca ggacatgtac
    54241 gctgccattg atggcttaat tgataagctg gcacgccagt tgaccaaaca taaagataaa
    54301 ctgaaacaac actaattgtc cgggcaatta gcatgtgcat ggcggcctgt tgtgcggcac
    54361 aacgggccat ttgtacggtt aatgctccga gcctgttcca ctgtttgagt gggcaggttc
    54421 ttaggtgaaa ttatgacaaa taatgataca actctacagc ttagcagtgt tcttaacagg
    54481 gaatgtacgc gaagccgcgt tcactgtcag agcaaaaaac gcgccctgga aatcatcagc
    54541 gagctggcgg cgaaacaact tagcctgccg cctcaggtgg tttttgaagc tatcctgacg
    54601 cgggaaaaaa tgggcagtac cggtatcggc aatggtattg ccattccgca tggcaaactg
    54661 gaagaagata ctctgcgcgc cgttggcgtt ttcgttcagc tcgaaacgcc tatagctttc
    54721 gatgccatcg acaaccaacc ggtggactta ctttttgccc tgctggtgcc ggcagaccaa
    54781 actaaaacgc acctgcatac tctgtcgctg gtggcgaaac gtctggcgga caaaaccatc
    54841 tgccgccgtt tacgcgcagc ccagagcgat gaagagctgt atcaaatcat tacggatacc
    54901 gaaggtactc cggatgaagc gtagttattc ggtaatgtct cttttagacg ttgtgaggag
    54961 aaacagtaca tggtactgat gatcgtcagc ggacgttcag gttcaggtaa atctgtcgcc
    55021 ctgcgtgcgc tggaagatat gggtttttac tgcgtggata accttcccgt agtgttgtta
    55081 cccgatctgg ctcgaaccct ggccgatcgc gagatttctg ccgccgtcag cattgatgtt
    55141 cgtaatatgc cggagtcacc agaaatattc gaacaggcga tgagtaacct gcctgacgct
    55201 ttctcaccgc aactcctgtt cctggatgcc gaccgtaata ccttaattcg tcgttacagt
    55261 gacacgcgcc gactgcatcc gctttccagc aaaaacctgt cgctggaaag tgctatcgac
    55321 aaagaaagcg atttgctgga gcctctgcgt tcgcgagcgg atctgattgt cgatacctca
    55381 gaaatgtccg ttcacgagct ggctgaaatg ctgcgtaccc gtctgctggg taaacgtgaa
    55441 cgcgaactga ccatggtctt tgagtctttc ggcttcaaac acggtatccc tatcgatgca
    55501 gattacgtct ttgacgtgcg cttcttgccg aacccgcact gggatccgaa actgcgtcca
    55561 atgacaggtc ttgataaacc tgtcgccgcg ttcctcgacc gccacacaga agtacacaat
    55621 tttatctacc agacgcgaag ctatcttgag ctatggttac ctatgctgga aaccaacaac
    55681 cgtagctact tgacggtcgc cattggttgt accggcggga agcaccgttc ggtgtatatt
    55741 gcagagcaac tggcagacta cttccgctcg cgcggtaaaa acgtccagtc acgccatcgt
    55801 acgctggaaa aacgtaaacc atgaccgtca agcaaactgt tgaaatcaca aacaagctgg
    55861 gcatgcatgc ccggcctgca atgaagctgt ttgaattaat gcagggtttt gacgctgaag
    55921 tgctgttacg taatgacgaa ggcaccgagg ccgaagccaa cagcgttatt gcgctgctga
    55981 tgttggattc tgccaaagga cggcagattg aagttgaagc gaccggccca caggaagagg
    56041 aagcactggc cgccgttatc gccctcttta actctggttt tgatgaagat taatcttcat
    56101 cactttgaca tacaactccc ttcaaactcc ccccgacaat aagaaaatca cgtactgaaa
    56161 tcgttctcaa tcaacgtcat ttgtacattt tgtgcgcttt tcactttcag aagaacctta
    56221 agaaaacctt aagaggcatt gtttaggttt tgtttaagtt aatcgaccat actggagatc
    56281 gtcagaaaat atttccagga gatggcatga ttcgcttatc tgaacaaagt cccctgggca
    56341 ccggaagaca tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt atcaagattg
    56401 tctaccatcg tggcgatggt ggcgataaag agatccgccg tgagttaaag tactacgcgt
    56461 atcttggtcg ccggttaaaa gactggagtg gaataccgcg ctatcacggt accgttgaga
    56521 cggattgcgg aaccggttat gtctacgatg tgattgcaga ttttgacggc aaaccctcca
    56581 ttacgctgac cgaatttgct gaacaatgcc gttacgaaga agacattgca caactgcgcc
    56641 agctactgaa acagctgaag cgttatttgc aggataaccg tatcgtgacg atgtcgctga
    56701 agccgcagaa tatcctctgt caccacatca gtgagtcaga ggttatcccg gtcgtctgcg
    56761 acaacattgg tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc
    56821 gtaagcaaga gcggttatgg aaacgattta tcgcccaacc ggcgctggct attgccctgc
    56881 agaaagatct gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc gaagcttaat
    56941 ccagctggtg ttgttgcata aacggctcac cgcctaactg atacatctgc cgtaaaatcc
    57001 acgcctgacg gctacgcacg tagcccgatg gcgaggagac tttgaaacga agtggattag
    57061 gtaatacagc tgccagtaat gcagcttccg accgggtaag tttgctcgcg ggtttgtgga
    57121 aataacgttg tgccgcagct tcgacgccaa acacaccgtc gccaaattcg gcgatattca
    57181 ggtaaaccgt caggatacgc tttttgctcc agactgtttc tatccccagc gttaatccag
    57241 cttccagccc ttttcgtacc cagctacgcc catcccataa aaagagattt ttggccgtct
    57301 gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg tgcgccaggg
    57361 ctttctcaat ggaagcgaca tcaaagcccc ggtgctcagg aaatttctga tcttctgcgg
    57421 caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag tcagaatgtg
    57481 ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc attaccgctg
    57541 agaagggaac aggcgcaaca ctaaacaacg cgatgccccc gccccagaaa acggcgagga
    57601 caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag cggcttttac
    57661 tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg ccgcttctgc
    57721 aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt catcaaccac
    57781 gatatcatcg acaggacagg gcacatgctc cgcgcccatc tcttccagca cttctgcggt
    57841 atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg gcagcatcgc
    57901 cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt gtgccagcgc
    57961 ctttaattca cggtcaacgg tgcattcgct accaagagtg gcaaaattgc ttaaattctt
    58021 cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat cggcctgggc
    58081 cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacgt tgcgcgtttc
    58141 cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat ccggtgcaaa
    58201 gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat
    58261 ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct tcattgtgat
    58321 catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt acgcatcaca
    58381 catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg cgtgcggcct
    58441 gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag ccaagatttc
    58501 cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt ttagtggctt
    58561 ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc atctcttcaa
    58621 tccattcacc gacgttatct tcccaggcag gaaggtcagg agactgaatt tgctgaccca
    58681 gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta tgtccttcct
    58741 caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg acatagcccg
    58801 gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct acaagttcga
    58861 gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc tcttctgtcg
    58921 tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc gcggttagcg
    58981 ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg tactcttgtt
    59041 tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg cgcggataac
    59101 gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga atatccagca
    59161 acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg gtcatggcga
    59221 catcaacgct gttacctaat ttttccagca cagaacgcgc aacaatcacg ttcagttcaa
    59281 tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat
    59341 catcgacctc ttctgctacc gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc
    59401 cctgttcgct ggtaaccgta atatcgccgc ccatattttt cgccagacga cgagaaacgg
    59461 ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc acctggtaat
    59521 acatggcgaa aattttatcc agctcatcct gcggaatgcc gataccagag tcttccactt
    59581 caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg ccttgctggg
    59641 tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc gtcccgtcgg
    59701 taatgacctg atgcggtaat ggcagcgtcg gctccaggtt aaagcgcaat cctttttgtt
    59761 gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa tcaaccggtt
    59821 gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata tcgttaaaga
    59881 tattccccag cgtgacggcc gaaacatgga tggtcttgag atatttttcc tgctcggcgg
    59941 tgagttcggt atccagcaga atgcggctca gaccgacgat accgttcagc ggtgtacgca
    60001 attcgtgact gatggtggag ataaacgtcg ttttgtcgcg gctggcccgt tcaagcgcat
    60061 cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac
    60121 ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc ccgtccgggt
    60181 aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt tcatcggttt
    60241 caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg tgaaccagtt
    60301 gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa aactctttat
    60361 cttcgttacg ataaaaaacc aggtcgggtg aagcatcaag gaaggaacgt aagaatgagg
    60421 attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt ttcagttggc
    60481 cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc tgatttagct
    60541 gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc atctcctcca
    60601 gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg acgaccaccg
    60661 atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa cgaataacat
    60721 caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc acaatggcaa
    60781 gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac ttcatcatca
    60841 ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc cttcacgaca
    60901 acctgcccca ataataccca attctgacag ttacgttata aattgtgtga gaaatgcgga
    60961 attcccctca acgacctact ccgttggagg tagccacgga cgtcccagcg ccgatccctg
    61021 tacgccatgt tcattcagat agcgatccag ctcgaccatt cccgtccatc gattttcgca
    61081 ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt ggtagatcac
    61141 ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtgtaatcct ccagttcaat
    61201 accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca caatatgcag
    61261 cggatgcagc tttatgccgt ccacgccggt ttcaaccact cgctccagcg tttgcaaaca
    61321 ttcagcctgc ccttcaccag gcaggccgac aattaagtgg gaacacacct tcagcccgcg
    61381 ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc ggttgatgcg
    61441 atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt cgtaaccctg
    61501 gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg ggcgggtacc
    61561 aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag aacgcagcac
    61621 ctgaacttcc gcaaaggtgc tagtatacgc ctgaaagtag gccagatagc gtttagcgcg
    61681 gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct gctgcgcttc
    61741 atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg taccgtcacg
    61801 gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc cataacgacg
    61861 ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct gatagaccgc
    61921 gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga cctggatcaa
    61981 tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa tttcatgcat
    62041 aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct tatggatttt
    62101 aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa tgctggtttt
    62161 ctcgtcagtt caaggtagga taagggttaa cacgccttta tgacagtcag gaattgactg
    62221 tttctctaac gacttccctt ttagccttaa agataaaatt cattttaatt tcagttgctt
    62281 aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat ttgacctgta
    62341 tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca gcctgctcat
    62401 catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa
    62461 caagggggcg aatacgaggc gcgcgtatga cacgcaaccc ccgtcgccac gctctttctg
    62521 tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag gttcacgata
    62581 tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc gcccacatag
    62641 aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc
    62701 accgtggcgc gattctcgcc gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa
    62761 aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta gcaaaaaact
    62821 acgctgtcgg gatgctcttc ctgaataaag atcctgaact cgcggctgcc gcacgccgca
    62881 tcgttgaaga agagctgcaa cgcgaaacct tgtcgattgt gggctggcgt gatgtcccca
    62941 ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccccgcatt gagcaaattt
    63001 tcgttaacgc tccggcaggc tggcgtccgc gggatatgga gcgccgtctg tttatcgccc
    63061 gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt agcctgtcga
    63121 atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc ttttatctgg
    63181 atcttgcgga cctgcgtctg gaatcggcca tctgcctgtt ccaccagcgc ttctccacta
    63241 acaccgtacc gcgctggccg ctggcgcagc cgttccgcta cctggcgcat aacggtgaaa
    63301 tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa ttccagacgc
    63361 cgcttatccc tgatctgcac gacgccgcac cgttcgtcaa cgaaaccggc tctgactcca
    63421 gttcgatgga taacatgctg gaactgctgc tggcaggcgg gatggatatc atccgcgcca
    63481 tgcgtctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg gaactgcgtg
    63541 ccttctttga ctttaactcc atgcatatgg agccgtggga tggcccggcg ggcatcgtga
    63601 tgtccgacgg tcgttttgcc gcctgtaacc tcgaccgtaa cggtctgcgt ccggcgcgct
    63661 acgtcatcac caaagataag ctcatcacct gcgcctctga agtcggtatc tgggattacc
    63721 agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg gttatcgaca
    63781 cccgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa agccgccatc
    63841 catataaaga gtggatggag aaaaacgtcc gcagactggt accgtttgaa gatctgcccg
    63901 atgaagaagt gggtagccgc gaattggacg acgacacgct tgccagctac cagaaacagt
    63961 ttaactacag tgcggaagag ctggactccg taattcgcgt actgggcgaa aacggtcagg
    64021 aagcggtcgg ttcgatgggc gatgataccc cattcgccgt gctctccagt cagccgcgca
    64081 ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca atcgacccgc
    64141 tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg aacgtctttt
    64201 gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg ctctactccg
    64261 atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg ctggatatca
    64321 cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctgtgc gacaaagccg
    64381 aaaaaatggt acgtagcggc accgtgctgc tggtgctctc cgaccggaat atcgctaaag
    64441 atcgcctgcc ggttccagcc ccgatggcgg ttggcgcgat ccagacccgt ctggtcgatc
    64501 aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc gatccgcacc
    64561 acttcgccgt gttgctgggc ttcggcgcga cggctattta tccatacctt gcctatgaaa
    64621 cgctgggccg cctggtagac acccatgcga ttgccaaaga ttatcgtacc gtgatgctca
    64681 actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca
    64741 ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctgcac gatgatgtag
    64801 tgggcctgtg cttccagggg gcggtcagcc gcattggtgg ggcgagcttt gaagacttcc
    64861 agcaggatct gctgaacctg tcgaaacgtg cctggctggc gcgtaagccc atcagccagg
    64921 gcggcctgct gaaatacgtc cacggcggcg aataccacgc ttacaacccg gacgtggtgc
    64981 gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa tacgcgaagc
    65041 tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg ccgggtgaaa
    65101 acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc tttgataccg
    65161 ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg gaagcgatga
    65221 atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg cgctatggca
    65281 ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt actccggcgt
    65341 atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag ccgggcgaag
    65401 gcggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc tattcggtgc
    65461 ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc gaggacttag
    65521 cgcagctcat tttcgacctc aagcaggtta acccgaaagc gatgatctcc gtgaagctgg
    65581 tttccgaacc gggcgtaggc accatcgcga ctggcgtggc aaaagcttat gccgatttaa
    65641 tcaccatcgc aggctatgac ggtggcaccg gcgcaagccc gctgtcgtca gtgaaatacg
    65701 caggttgccc gtgggagctg gggcttgttg aaacccagca ggcgctggtt gccaatggtc
    65761 tgcgtcataa gatccgtttg caggtcgatg gcggcctgaa aaccggtgtt gatatcatca
    65821 aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccaatg gtcgcgctcg
    65881 gctgtaaata tctgcgtatt tgccacctga acaactgcgc aacgggtgtg gcaactcagg
    65941 atgacaaact gcgtaagaat cactatcacg gcctgccgtt caaggtgacg aattactttg
    66001 agtttatcgc ccgtgaaacc cgcgagctga tggcacagct gggggtaacg cggctggtgg
    66061 atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc aaacagcaga
    66121 aactggcgct gtcgaagctg ctggagaccg ccgaaccgca tccaggtaag gcactctact
    66181 gcaccgaaaa caacccgccg tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg
    66241 cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatc cgcaataccg
    66301 atcgctccgt gggcgcgtcg ctttcaggct atatcgccca gacgcacggc gatcaggggc
    66361 tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt ttcggtgtat
    66421 ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat gtcggtaaag
    66481 gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc cgcagccatg
    66541 aagcaagtat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt ctgtatgccg
    66601 caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc gtggtagaag
    66661 gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt ctgggtaaaa
    66721 ccggcgttaa cttcggtgcg ggcatgaccg gcggcttcgc ttacgttctc gatgaaagcg
    66781 gtgatttccg caaacgcgtt aacccggaac tggtcgaggt cttaaacgtt gacgatctgg
    66841 cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat actggctctc
    66901 agcgcggtga agagattctg gcgaactggt caaccttcgc cactaaattt gcgctggtta
    66961 aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc gcagcagagt
    67021 tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat caatttatcg
    67081 acctgcagcg cgttgatccg cctaagaaac cgctgaagat ccgcaaaatt gagtttgttg
    67141 aaatttacga gcagttttcc gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt
    67201 gcggcaaccc atactgcgag tggaaatgtc cggtacacaa ctacatcccg aactggctga
    67261 agctcgccaa cgaggggcgt atttttgaag cggcggaact gtcgcaccag accaacaccc
    67321 tgccggaagt ttgcggacga gtctgcccgc aagaccgtct gtgcgaaggt tcctgcactc
    67381 tgaacgatga gtttggcgcg gtgaccatcg gcaacattga gcgttatatc aatgataaag
    67441 cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt aaaaaagtgg
    67501 cgattatcgg cgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg cgtaacggcg
    67561 taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc ttcggtattc
    67621 cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc accggcatgg
    67681 gtattgaatt caaactcaat accgaagtgg gccgcgacgt gcagctggac gatctgctga
    67741 gtgattacga tgccgtcttc cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg
    67801 aaaacaaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac
    67861 agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa ggcaaacgcg
    67921 tggtggtcct tggcggtggc gacactgcga tggactgcgt gcgtacgtcc gtgcgccagg
    67981 gagcgaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc
    68041 gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc cagccactgg
    68101 ggattgaagt gaacggtaac ggcaaagtca gcggcgtaaa aatggtacgt accgaaatgg
    68161 gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc gaacatatcg
    68221 ttccggcaga tgcagtgatc atggcgtttg gtttccgtcc acacaacatg gaatggctgg
    68281 caaaacacag cgtcgagctg gattcacaag gccgcatcat cgccccggaa ggcagcgaca
    68341 acgccttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg
    68401 atctggtggt aaccgctatt gccgaaggtc gtaaggcggc agacggtatt atgaactggc
    68461 tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg ctgaaattct
    68521 cctttctttc tggaaagcgc ctcgcataat ctgaagaaaa ccaatgtaat acgctcatct
    68581 ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg cagcgccata
    68641 taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac cagcaaatcc
    68701 acgacggtat tattcacctg gtcgatagcg cccggacgga aacggtacgt agcgttaacg
    68761 cgctgatgac cgcgacatac tgggaaattg gccgacgaat tgtcgaattt gaacaaggtg
    68821 gcgaggcaag ggctgcgtat ggtgcgcagc taatcaagcg actatcaaag gatttaagtc
    68881 taaggtataa gcgtgggttc tctgcaaaaa acttacgcca aatgaggctt ttttacctct
    68941 tttttcaaca tgttgaaatt cgccagacag tgtctggcga attaacacca ttgggaattc
    69001 cgcagacacc atctgcagaa ttcccatcgg caaagatttg gcagacgctg tctgccaaat
    69061 tgagaggctg tttgatattt ccacagcttt tgtattgcat ctcctatttt cggtttgtat
    69121 attcatgcaa ataaacgaag gaaagttgcc atgatttaca ttgaacagct tgaattaatc
    69181 cataaaagtg gtgatgtgtt gtatccagta aaaataacca gaaaatcatc aggaaaaact
    69241 gccttccatc ttgttccatt cggcttaaat aagactcacg accttctaga ggtggaagat
    69301 gcttcagaag caattcggct ggtgattgat gaacgtcatt ccattcgctg cagtacactt
    69361 acagcaacta ttacgaataa aaagggcaag cgaattaagc ggacaggtat atacagtatc
    69421 aagggtgtta acataaaaga atacaacgtc aggtaatggc acagagcggt ccgtcagttc
    69481 aggtttggct ctgtgccaaa gatgggtcag gtcacacctg atctaataaa tttaagccat
    69541 taaatcagcc ttaaccttca ccaccacctt tttaatctca cccggctcgc cgacaacgca
    69601 tcctggttta tgcggttcac ttggcataaa cacggcgaac attcccggtt ttaagatgat
    69661 ggcttgctcg ttatcaatgg tgctgcaaag ctgataatca tcctcatggt ggaactcttc
    69721 acactggcgc gcagtgcctg ccatgccaaa cagaatccgt tcctcaccgt ttaataacag
    69781 ctggatatca atgtattgct cgtgcaattc cgcttttttc tcgacgggcg attgagtgtt
    69841 aaacgtcatg acattcataa agatattgtc gccctgtaat tcgtaacgac caggcgcttt
    69901 ttcttgcggt ctggcagcta atgccagcgt taacgcgtcc tgtaacgcag gatgtaaccc
    69961 agcagacggt aatgactgta cttcacccat catcataatt tttctccctg ggccaacagc
    70021 gcagccccaa gtaaacctgc atcgtggcgg tagtgcgccg ccagtaaatc aacatgaaat
    70081 gccgctggct cctgcgccag atacgtttcc accaacgcca gatacccttc tgccagacca
    70141 acgctgccac cgaccaccac gcactggcaa tcagttgtgg ctttaatatc agcgatcagc
    70201 cttgccagca catgtgcgga gcggtgaatc agttgctgcg cctgctcgtc gccctgcccg
    70261 gcgcgtgtga aaatagttct cgcatccgcg cccatgagtt caccttgcgc tgccgctgca
    70321 ataccgcgac cagaagcaat cgcttccacg caacctgtgc gtccacagcc gcagactggg
    70381 ccgtgtggat cggcaagcgt atgcccgatg tgccccgcca gaccgccagg gccggtaagc
    70441 agtttaccgc cgctcactac accgccgccg acgccagtgg aaacggtgat aaacaccata
    70501 tcggttatat cgccttccag cgcctgatac tctgcccatg ctgcggcctg cgcgtcgtta
    70561 atggcaatgg tcggcaaatt ggaaagttgt tccagcgttt tgactaacgg aaagtgtagc
    70621 aatccaccaa gattatgcgg attcagcgcc agcaagctgc cgtcgcgaat aattccggtt
    70681 gaagcgatgg caacctgctg cgcatgagct tgcaacgggg cgactaacgc ggctaaggca
    70741 tcacgcaacg cctgcggagt ctggctggct ggtgtcggaa gttctcggcg atcgcggatc
    70801 tgcccgtcag cgccaatcag cgcggcggca agtttagtac cgccgatatc aatcgccaac
    70861 gtggtcatag caccgccttt ttcatcgctg tgttgtacca ctgacaaatg tgttcaagac
    70921 gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg gcgcatcgca tctgccgcct
    70981 gagcaggcgt gttgtaacgc ccttcggcaa tcacccgaca tccggcgtcg ctcaacgttt
    71041 tcaccagcgc cagatccggc tcttctggcg tttcaggcgt ggtatagcca gaaagcgtag
    71101 tgccaataat ttcggctccc agcttttggc atgccaggcc gtcttccggc gttgagcagt
    71161 cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag cagcgtttca acaggcaccg
    71221 gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc gcccgcctgc gccagcgcat
    71281 caacatcttc aatataggcc gtgatgcgta ccggagaatc ctccagatcg cgtttcacaa
    71341 ttccaataat cggcacgctc accaccgcac gcgtggcttg cagatttgcc acaccttcaa
    71401 tgcgaatggc aaccgcgccc gcctgttctg ccgctaatgc catggcagcg acgatttcgg
    71461 gtttatcgag cgggctgtcc ggaaccggct ggcaggagac aatcaggcca ccgttagcag
    71521 cgattttttg atccagttgt gcaagtaacg acatacatct tcccttagcg aaaggcccgg
    71581 tacagagacc gggcaacagg attaactttt ggttttgact aaatcgtttt tggcgctgcc
    71641 aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg tcatgagtac gcaatgcttc
    71701 cgggcgcagc caacgctgaa cgcgggaagg catatccaga ccaatcagca ggatcaccac
    71761 aaatgtcaga ctgaacgata gcgatgccag cgcagtaccc agatccagac gttgagcgat
    71821 caacgcgccg atgattgggg ccagtgcgcc gcccaatgcg ccaacgttgt aggtaaagcc
    71881 caggcccgct gcacgctggt cggtatcgaa ataaccgcca atcagtttcg gtaagatccc
    71941 ggcgatccct tgtccaagca tttgctggaa gaacagtaac agaccgagca cccagacgtt
    72001 tgcgccgcca atcgcaaata ccggaataat caacagctgc gaggccaaca ggctacaaac
    72061 gtaggctttg cgggttccca gccagtcacc gaggaagccg ctgacacagc accccaccgc
    72121 agccccaaag ccgctaaaga acagcacatt ggcgacagta tgcgggttat aagccagatc
    72181 ggttttcaga tacgttggca gcagcgcctg aatcggccat gagtagagga aagcaaacaa
    72241 cacgaccacc atcagcatta cgcccgttgg ccagcgttta cccgtacttt gcaccataaa
    72301 gctgataaag attgcggcgc ataacagccc aagaacagcg acgatcgcgg cattttgcag
    72361 gttaccggcg aagcaaaacc acagcgcagt agccgccgcc agtgtcatta cgatattggc
    72421 aatgcgatgt tcaccacggt agagaatatc taccatcgtg cgtactggtg ctttacctgc
    72481 atgcttctct ttccagtctt ccgcttccgg gatgttttta cgcagccaga gagcaaagat
    72541 gataggcaaa atgccgataa agaacagcgc acgccagccc cagaccggaa ccaccaggct
    72601 atagacctga gcggcaacga cggcccccac agagaagcct gaaatcagaa aaccactggc
    72661 tttgttacgc agatgttttg gccagctttc aatgacatag gtggcgctgg aaccgtattc
    72721 acccgccatc cccatgccga tgaccagacg cgcgataaac atggtgatgt agcctggcgc
    72781 aaagccgcag gccagcgtcc cggccgagaa gagaacgatg ctggtgacca ttgccagacg
    72841 acgcccgtag cggtcaccca tagcgccgag catcaggccg ccgaaccagc gagagataaa
    72901 ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc ccgaattcac cctgtacttc
    72961 ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca agcagatatc ccaaccaggc
    73021 agcggaaaat gcgcgccatt gtgcacggtt gagatggcga taccacggga tattctgggt
    73081 tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc acacgctttg ctataaaaat
    73141 tgtagggtac agatgcgttt atttcccctc acccggtagg ggcgagcgag gggaaacaac
    73201 tcacccgcgc tcttgcatca actgctgggc cagcgccttc agttctggca gatatttttc
    73261 atctaccggt ccaaacggtt tgcggcacag cggcacagaa acgacatcca tataatggag
    73321 gacagttttc aggccgcgga atacgcccgt tttgatcagt aaatcaatga ctttattgca
    73381 ttcagtttgc agtttctgcg cggtctggat atcgccttct ttcagcgcct taacgatccc
    73441 ctgatagcgc cagcccatga tgttgtaggt actaccgata ccaccatcag cgcccgccag
    73501 cagaccagag gcgaagattt cgtcgtaacc gttatagagc acaagatcag gatgttcacg
    73561 acggatctgc tccatctgat agagatcgcc agaggtctgt ttcagcgcac ctacgccagg
    73621 caatgtaaca agtgtgttga tctgatccag ggtcagtttt accccactca gggctggaat
    73681 gttgtacacc accatcggca aaccatccgc cgaatcaata attgcccgat agtgatcgca
    73741 gtgttcttca aagctgaaag gatagtagaa cggcgtgacg gcggagacgg catcgaagcc
    73801 ataacgttta gccgatgccg caagttgttg gctttcggcg gtgctgacgc aaccgacgtg
    73861 ggcgatgagt ttaatcttac ctttcgcctc ttcggcgacg atttccagta cctgttcacg
    73921 ctcggaaagg ctttgtacaa aggcctcgcc ggtcgaacca cccacgtata aaccgtcgat
    73981 gccctgctga atattgaact gaaccaggcg acgcagactc gctttatcca gtgcttgttg
    74041 ttggtcaaaa ggagtcagga gtgcagccat tacgccacgt aaattcgttg ccataaatac
    74101 ctctgaagtg atgcttgtct gataaacgat atacctttat acctgttata ccagatcgat
    74161 taagcaacac cccatacaga aagcttataa tgcgatctgc ttcactaaag tggcattatt
    74221 tctttttgtt ggtggtctga ccgaaagcgt gccaggtagc agagacgctg ttgagatgcg
    74281 attgcaacgc acgatcggct tcgtcaggat catgacggcg gatcgcatca acgatcgcaa
    74341 tatgctgttg ataactaacg ttgttatgtt cgtgcagtgc ctgatcggta accgttgggc
    74401 gtgcggcaat aagccagtcg agcagggcaa cgtggatcgc catgaagatt gggttaccgg
    74461 ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat
    74521 ccagcgactg actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg
    74581 catgttcagc cgcatagcgc accagactgg attcaaagaa cagacgtaat tgttcgaaat
    74641 gggcaatccc accgggatga gaaaggaaat ctttcgccat gccggaaagc tcaccgatga
    74701 tagtgtccgc agaaggacgc gagacgcgag cgcgttcgcc gttgtttatt tgcaccagac
    74761 ctttgcgttt taacgctgcc agcgcttcac gcaccgaagg acgcccgacg ttaaagaacg
    74821 ccatcagttc gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca
    74881 tctgttccag ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctac
    74941 gcaagttgcg accaattgca ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa
    75001 ggcccattct gtaaggtcag tgtgattaac atcatcagtg acatcctatc acaggattga
    75061 gagtagggga aaatggcagg tttttctctt tgtgcctcat cattaccata attaacggaa
    75121 taattaacta ttgcgaaaaa ttaatgtaac gcagataaaa acatcccgtt tgaattattt
    75181 ataagactat tcacgagcat tatgaatatt atgaatgcgt tcttgcaaaa taatcataag
    75241 cgcatatttt ttaatgaaaa attatctcac ctacaattaa aaacacgaca tccgcaccat
    75301 aaatagactt gcaaaaaata taacatcatt gttttcaatc tgccgtttat gggattgacc
    75361 gttttctttt gacacggagt tcaacaatgt tcggcataat tatatctgtc atcgtattaa
    75421 ttacgatggg ctatttgatc ctgaaaaact acaaacctca ggtggtgctg gctgccgcag
    75481 gtatcttcct gatgatgtgc ggtgtctggt tagggttcgg tggtgtactc gagcccgcca
    75541 aaagcagcgg ctacttgatc gtcgatattt ataatgaaat cctgcgcatg ctgtccaacc
    75601 gcattgccgg attggggcta tcgattatgg cggtgggcgg tatgcccgct acatggagcg
    75661 cataggggcc agtcgcgcga tggtgagctt gttaagccgc ccgttaaaac tcattcgctc
    75721 accgtatatt attctgtcgg caacttacgt catcggtcaa atcatggcgc agtttattac
    75781 cagcgcctcc ggtctgggta tgttgctgat ggtcacctta tttccgacac tggtgagtct
    75841 gggagtaagt cgtctctctg cggtggcggt tatcgcaacc acaatgtcca ttgagtgggg
    75901 aattctggaa acgaactcca tttttgcagc ccaggtcgcg ggaatgaaaa ttgccactta
    75961 cttcttccac taccagcttc cggtcgcctc ttgcgtcatt atctcggtgg cgatctccca
    76021 ctttttcgtg caacgcgctt ttgacaaaaa agataaaaat atcaatcacg aacaagcaga
    76081 gcaaaaagct ctcgataatg tcccgccgct ctattacgcc attttacctg tgatgccgtt
    76141 aatcctgatg ctcggctcgc tgttcctcgc ccacgtcggg ctgatgcagt cagaactgca
    76201 tctggtggtg gtgatgttac tgagtttgac ggtgacgatg tttgttgagt tcttccgcaa
    76261 gcataacttg cgcgaaacaa tggacgatgt gcaggcgttt tttgacggca tgggtacgca
    76321 gtttgccaac gtggtaacgc tggtggtcgc gggtgaaata tttgcgaaag gcttaacgac
    76381 gattggcact gtcgatgcgg ttatcagggg tgcggagcat tctggtctgg gcggtattgg
    76441 cgtgatgatt attatggcgc tggtcattgc catttgtgcc attgtgatgg gctctggcaa
    76501 tgcgccgttt atgtcatttg ccagtcttat tccgaatatc gcagccggac tacatgtacc
    76561 agcggttgta atgattatgc cgatgcattt tgccacgacg ctagcgcgcg cggtttcgcc
    76621 gattactgcg gtggtggtcg ttacgtcagg aattgcaggc gtttcgcctt ttgcggtggt
    76681 gaagcgaaca gcgatcccca tggcagtcgg tttcgtggtg aatatgattg ccacaatcac
    76741 gctattttat taagccatta aaagacaaaa caggccacca gggcctgttt ttgtattact
    76801 tcacaacgcg taatgccggt cgaccaccgc gtggtggctg cggaggttca tcgtcaggat
    76861 gagtgtcatc atcgtgatct ggcttgtcgc catcaataac cgacataacg gtttcgttgt
    76921 ctgccgatgc ctcttcatca ttcatgatgc tggtatcttc atcgtaggca gcttcaggct
    76981 caaacattgt gcctgcgcca ttttcacggg cgtagatagc cagcacggca gccagcggca
    77041 cagaaacctg acgcggaatg ccgccaaagc gcgcgttaaa gcgcacctca tcattcgcca
    77101 gttccagatt gccgacagca cgcggcgcaa tgttgagtac gatttgcccg tcacgcgcat
    77161 attccatagg aacctgcacg ccagggagcg tcacatccac cactaggtgc ggcgtgagct
    77221 ggttatccag caaccactca tagaatgcac gcagcagata gggacgacgt ggtgttagct
    77281 gtgacaaatc catacagatt aactccggcc cagacgcatt tcacgttctg cttcagttaa
    77341 agaagcaagg aaagagtcac gctcaaagac gcgggtcata tagcctttca gctctttcgc
    77401 acccgggccg ctgaactcga tgcccagttg cggcagacgc cacagcagcg gagcaagata
    77461 gcaatcgacc aggctgaact catcgctcag gaagtacggc ttctgaccga agaccggcgc
    77521 aatcgccagc agttcttcgc gcagttgctt acgtgcggca tctgcttcag aagctgaacc
    77581 gttgatgatg gtgttcatca gcgtgtacca gtctttttcg atgcgatgca tgtacagacg
    77641 gctttcaccg cgagctaccg ggtaaacagg catcagtggc ggatgcggga aacgctcatc
    77701 cagatattcc ataatgatgc gagattccca cagggtcagc tcacgatcca ccagggtcgg
    77761 aacgctctga ttcgggttga ggtcaatcag atcctgaggc ggattgtcct tttccacgtg
    77821 ttcgatctcg aaacttacac ctttctcagc cagcacaatg cggacctgat ggctatagat
    77881 gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga cagccatgaa
    77941 aacctccagg tatagtcaga atttttactg ctaccagcca ccaggtggcc agtcagaagt
    78001 tgtgttaccc agtaaggaac gactctcttt gttcgaaaat caaacaaaaa atgagcaata
    78061 cccgacattt gggcagaaaa ttggatgata gtttaccaga ttttgcgacc tttgtggtga
    78121 gtcgatgccg gaaatgggga aaaagagatg cgctttagtc tgaaatagtt gacttagtcc
    78181 cttattggcg atgtggtttt tgttttacct gtctgtcagg tggcagcaaa aagcaacttt
    78241 ccagttttta cgctgattca gattttagct ataaaaaaac ccgccgaagc gggttttttc
    78301 gaaaattgtt ttctgccgga gcagaagcca attaacgttt ggagaactgc ggacgacgac
    78361 gtgctttacg cagaccgact ttcttacgtt caacctgacg agcgtcacga gtaacgaagc
    78421 cagctttacg cagttcagaa cgcagggact cgtcgtattc catcagagcg cgggtgatac
    78481 cgtgacggat cgcaccagcc tgaccagaga taccaccacc tttaacggtg atgtacaggt
    78541 ccagtttctc aaccatgtcg accagttcca gcggctgacg aactaccatg cgggcagttt
    78601 cacgaccgaa gtactgttcc agagaacgtt ggttgattac gattttaccg ttgcccggtt
    78661 tgatgaaaac gcgagctgcg gaacttttgc ggcgaccagt gccgtagtat tgattttcag
    78721 ccattgccta taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc gcgtggttgt
    78781 gctcgttacc cgcgtaaact ttcagtttac ggaacatagc acgacccagc gggccttttg
    78841 gcaacatgcc tttaaccgcg atttcaatca cacgctcagg acggcgagca atcatctctt
    78901 caaaggtcgc ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac actttgtcag
    78961 tacacttgtt gccggttaca gcaactttgt cagcgttcag aacgatgatg taatcaccgg
    79021 tatctacgtg cggagtgtat tccgctttgt gcttaccgcg caggcgacga gccagttcag
    79081 tagccagacg gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg cgttttacgg
    79141 tttctggttt agctgtaaaa gttttcatta aaagcttacc caataaatag ttacacgttg
    79201 gtgaacaccc aaacgtcttc aattgttgag gttcacacga caaagtccgg caaacctacc
    79261 ccttcgaata gcctatgcca gcacacaaaa agttttggga aaaaactttc ttgtaacgtg
    79321 gggtcgcagg attatagaga agtcggggtc aaagatcgac ccctttttgt gatttgtgac
    79381 aggttttaac ccgccaaatg ctcgcgcttc agatactctt cgctttgcat ctcttgcaga
    79441 cgtgacaggc aacgctggaa ctcaaacttc agccgatcgc cctgataaat ttcatacagc
    79501 ggcacttctg cactcaccac taatttgaca tggcgctcgt aaaactcatc caccagcgca
    79561 ataaagcgcc gcgcttcgct ctccatcaac cgcgtcataa ctggtacatc aaacaacatg
    79621 accgtatgaa agaggcgtga gagcgcaata tagtcatgct ggctgcgggc atcgacacac
    79681 aaggtggtaa aagagacagc aagcgtctga ttctcagcgc ccattgtcgc cagcggtcga
    79741 tggttgattt ctaacgtcgg tgaattttcg cgtttcgctc ccgccagcgc caaccatagt
    79801 ttatccattt gtgcccgggt ttcatcgtgg agtggcgaaa gccacagatg cgcctgagtg
    79861 agtgtacgca gtcgataatc aacaccagcg tccacgttca ttacatcaca atgctgttta
    79921 atggcatcga ttgcaggcag aaaacgcgca cgttgcaagc catttcgata aagttcgtcc
    79981 ggcggaatat ttgacgttgc taccagggta ataccgcggg cgaacagggc tttcatcaga
    80041 ccgccaagta gcatggcgtc ggtaatatca gaaacaaaaa attcgtcaaa acagagcacg
    80101 tcagtttcgg ctttaaagcg atcggcaata atttccagcg gatcggtctg cccctgtaag
    80161 gcagttagct cttcgtgcac ccgcagcata aaacggtgaa agtgcaggcg ctgtttccgc
    80221 tctcccggca ggctttgata gaaaaggtcc atcagccagg ttttcccgcg tcctacaccg
    80281 ccccacatat ataagccacg cactggcgta tgctttgtgt cttcgcgttt accccacagc
    80341 ttaccgaccc gcgccattag cccactcgtc ctgggggctg gtggcctgct attgatgagt
    80401 tcttgataaa taatttccag gcggctgacg gcctcttttt gaacgtcgtc gggttgatgg
    80461 ctgccttcat tgagcgcctt caggtattgc gatgttgggg taacgctttg catgatctta
    80521 ttattattcc ttgaataatc ggtgcgccgt tgttcacggt tgacgaaaaa aaggccgttc
    80581 tacactacgc gatatgcagt cgggattcca cttctgtgga attaacggtt atagtggcat
    80641 aatcagccgt aggcatggag cctgaagcca acaccctacg gaaacaaaag acaacgggag
    80701 atgttcatga cctgggaata tgcgctaatt gggttagtcg tcggcatcat tattggtgct
    80761 gtggccatgc gttttggtaa tcgtaaacta cgccagcaac aggcgttgca gtacgaactg
    80821 gaaaagaata aagctgaact ggacgagtat cgcgaagagc tggttagcca ctttgcccgc
    80881 agcgcggaat tactggatac catggcgcac gactatcgcc agctgtatca gcacatggcg
    80941 aaaagctcca gcagcctgct gccggaactg tctgctgaag caaacccgtt ccgtaatcgt
    81001 ctggcagagt ctgaagccag caacgatcag gcaccggtgc agatgcctcg cgactattct
    81061 gaaggtgcat ccggcctgct gcgtactggc gcgaagcgcg actaatttat ttttcgggcg
    81121 cagccattgc gccctcctct tctctccctc cccgactatc atttaatctg gtgtctcatt
    81181 gttagccgtc tgaaaattca ataacatcaa actgttttga atctcttttc ttatcattca
    81241 ggtacgagag caggaataat gaaaaaacaa acccagctgt tgagtgcatt agcgttaagt
    81301 gtcgggttaa ctctctcggc gtcatttcag gccgtcgcgt cgattccagg ccaggttgcc
    81361 gatcaggccc ctctccccag tctggctcca atgctggaaa aagtgcttcc ggcagtggtg
    81421 agcgtacggg tggaaggaac ggccagtcag ggacagaaaa tcccggaaga attcaaaaag
    81481 ttttttggtg atgatttacc ggatcaacct gcacaaccct tcgaaggttt aggttccggt
    81541 gtcatcatca acgccagtaa aggctatgtg ctgaccaaca accatgtgat taatcaggca
    81601 cagaaaatca gtattcagct caatgatggg cgcgagtttg atgcaaaact gattggtagc
    81661 gatgaccaga gcgatatcgc cctgttacaa attcaaaacc cgagcaaatt aacgcaaatc
    81721 gctattgccg actccgataa attgcgcgtc ggtgattttg ccgtagcggt cggtaaccca
    81781 tttggccttg ggcaaaccgc cacctctggc attgtttccg cattaggccg cagcgggttg
    81841 aatcttgaag gtctggaaaa ctttatccag acagatgctt ccattaaccg cggtaactcc
    81901 ggcggtgcac tattaaacct taacggtgag ttaattggca tcaacactgc aatccttgcg
    81961 cctggcggcg ggagcgtcgg gattggattt gccatcccca gtaatatggc gcgaacactg
    82021 gcgcagcagc ttatcgactt tggtgaaatc aaacgcggtt tgttaggcat caaaggcacc
    82081 gagatgagtg ccgatatcgc caaagccttc aaccttgacg tgcagcgtgg cgcgtttgtc
    82141 agcgaagtgt tgccaggttc tggctcggca aaagcgggcg tcaaagcggg cgatattatt
    82201 accagcctca acggcaaacc gctgaatagc tttgctgagt tgcgctctcg tatcgcgact
    82261 acagagccgg gcacgaaagt gaagcttggc ctgctgcgta acggcaaacc actggaagta
    82321 gaagtgacgc ttgataccag cacctcttcg tcggccagcg ctgaaatgat cacgccagcg
    82381 ctggaaggtg caacgttgag cgatggtcag ctaaaagatt gcggcaaagg tattaagatc
    82441 gatgaagttg tcaaaggaag cccagctgct caggcaggct tgcaaaaaga cgatgtgatc
    82501 attggcgtca accgcgatcg ggtgaactcg attgctgaaa tgcgtaaagt gctggcggca
    82561 aaaccggcca tcatcgccct gcaaattgta cgcggcaatg aaagcatcta tctgctgatg
    82621 cgttaatgtc gtaaaccggg catcaggctt acgtgtgatg tccggttaac tcgtggtatg
    82681 ctgctgccgt tccctttttt aatgacgcct ccatcatgtt tgtgaagctc ttacgttccg
    82741 ttgcgattgg attaattgtc ggcgctattc tgctggttgc catgccttcg ctgcgcagcc
    82801 ttaacccgct ttccactccg caatttgaca gtaccgatga gacgcctgcc agctataatc
    82861 tggcggttcg ccgcgccgcg ccagcggtgg ttaacgttta caaccgtggt gtgaacacca
    82921 actctcacaa ccagcttgag atccgcaccc tgggatccgg tgtaatcatg gatcaacgcg
    82981 gttatatcat caccaataaa cacgtcatca acgacgccga tcagatcatc gtcgccttac
    83041 aggatggacg tgtatttgaa gcattgctgg tgggatctga ctctctaacc gatctggcgg
    83101 tacttaaaat taatgccact ggcggtttac ctaccattcc aattaatgca cgtcgcgtac
    83161 cgcacattgg cgacgtagta ctggcgatcg gtaacccgta caacctcggg cagaccatta
    83221 cccaagggat tattagtgcc acggggcgaa tcggtctgaa ccctaccggg cggcaaaact
    83281 tcctacaaac cgatgcttcc attaaccacg gtaactctgg cggcgcgctg gtgaactcgc
    83341 tgggagaact gatgggcatt aacacgctgt cgtttgataa gagtaacgat ggcgaaacgc
    83401 cggaaggtat cggctttgcg attcctttcc agttagcaac caaaattatg gataagctga
    83461 tccgcgatgg gcgcgtgatc cgcggctaca ttggcattgg tggacgtgag atcgcaccac
    83521 tgcacgcgca gagcggtggt atagatcaac tgcaagggat cgtggttaat gaagtgtcac
    83581 ctgacggccc ggcggcgaat gcgggtattc aggtcaacga tctgattatt tcggtggata
    83641 acaaaccggc catctctgct ctggagacga tggatcaggt ggcagaaatt cgccctggtt
    83701 cggtgatccc ggttgtagtg atgcgtgatg ataagcagtt aacgctgcag gtcaccattc
    83761 aggaatatcc ggcaaccaat taagtcgagt gctcaaaaca aaaaaccgga gtctgtgctc
    83821 cggtttttta ttattcgcta atcaattact tattaacgaa ctcttcgccc agggcgatat
    83881 ctttcttgag cgtatccagc ataccttcca gcgcgttctg ttcaaatgcg ctcagggtac
    83941 cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga gagaagaaac
    84001 gggcgtactg accgtcgcct tcaacgtagg cacattcgac aacgccttgt tcgccctgca
    84061 gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac agggttgcag
    84121 acccgccacc ggctttcgct tcaaccacct cagtacccgc gttctggata cgtttggtca
    84181 gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc agcggcagaa
    84241 tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcttggc tgtttgcctt
    84301 tcagttccgc aacaaaggtg ttggaacgaa tgatatccag cgtggtaacg ccgaacagtt
    84361 tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaattgca actgtggtgt
    84421 taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca acttgctgta
    84481 ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc atacccggtt
    84541 tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc ggagtcgcat
    84601 cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga tcgacagcca
    84661 caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa ggcagttggg
    84721 tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg actgcgactt
    84781 tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct gccgcgacct
    84841 taatccacaa aattgccgtt tacaatgacc acatatcaag aatgtgtagt cacgcaagtt
    84901 tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt aacagtcgct
    84961 tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt cagccaaaac
    85021 aacatcaatt tgataacaat taatttactt ttaagcagaa tttgcatgcc gtgacgcagg
    85081 catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt atttcagcct
    85141 tcttcagggt tgactgtttg cataaaaatt catctgtatg cacaataatg ttgtatcaac
    85201 caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt taaagcattt
    85261 aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc cgcgttgcag
    85321 gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac caagtttggt
    85381 gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc tgaactgggt
    85441 gtaccaacca cctccagtcc attgaagaat ctggtgctgg atatcgacta caacgatgca
    85501 gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct gctggactca
    85561 ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat ctttaccacc
    85621 cctgctaacg gtttcaccgt caaagacctg tacgaagcga ttttagagct gttcgaccag
    85681 gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt atccctctca
    85741 accccctgct ttcccctgcg attaatttaa cgaatagtgc gttttactgc gacatgtcat
    85801 tcacataatg aatacatacg gtaaaaaaag cacattatgc aaaattcatt atctaattga
    85861 aaatactaga attaaaaata aatgaccgtg tttttaattc ttttttgtta ttaaaattca
    85921 catttttaac acttagtatc aactgaaaca gttagcgtgg tattaattag ttcaataatt
    85981 agtgtatgct tgattttgtg atatgggtca caaaacaaag gcccagctaa aagattatgt
    86041 cgaggtaaaa atcatgaaaa tcaaaaccac tgtagctgca ttaagcgtac tttctgttct
    86101 ctctttcggt gcattcgctg ccgactccat tgatgctgca caagcacaaa atcgtgaagc
    86161 aatcgggacc gtatccgtaa gtggtgtggc gtcttcgcca atggatatgc gtgaaatgct
    86221 gaacaaaaaa gcggaagaga aaggcgcaac ggcctaccag attactgaag ctcgtagcgg
    86281 tgacacctgg cacgctacgg ctgaactgta caaataaacc ctcatcgtct tgtccgacga
    86341 tattgccccc ggttcggggg ctttttttgc gctaatgacg aacattaaaa cgcaaatgcc
    86401 cttccagctc ttcctctgcc tcatcaaaca gcaatattaa cgcgccaaaa cggcgacgcg
    86461 ttttctctcc cagatgaaca aattcaatct caagtggtag cggcaggaca tcgttcatta
    86521 acacatccca tagtgagtcg agatcgcgta ccttatcttt cgccagacca aaggtttggc
    86581 taaagtcacg ataaaaatcc tcctgactct caatctcatc aaaatcaaag gtataaatat
    86641 tcatctattg ccaccgtcac gtttcgcggc gagttctgcc gtgcatcgct aagtatagcc
    86701 atgaaaaaac cgacactttt ggcgtcggtt ttgacctaac tatcggtcaa cgcatgttga
    86761 tacttatgga gcatacccgc cagccgatga accggttccg tcacctgcgg tggcgcttgc
    86821 cagatgcgta gcttttcctg gtagatttca agttcttcca gcaactggcc aaagtaccga
    86881 cgacgtttat catcgctacg ggcagatatc acatggtctg ctgtgcggcg catttgtcgg
    86941 tgaaacgccg ataaatcctc gttaaccggg atcggcgcat cacgcaggcg ctggtgcgcg
    87001 ataatcatcg tcagcgccag gcgaaatttc ggcaaatccc ctgggaactt attcatcagc
    87061 aaaaacagct gctgataaag tgccgggagg tggttctctt tacgacgtgc cacattggta
    87121 gtcatcgcgg aaacagcggc agaaacaaac tgattaagca gtacacgttc ggtcctgtcg
    87181 cgcgatttat cccgcacaag caaaataacg gtgaacgcga gcacacagcc gacgatttgc
    87241 cctaatgcgc tgtcgagaaa ctgactgaaa tggaaagtca tcgggttatc cagcacgata
    87301 atatttatgg tgctggccag cgcccccatc gagcccagtc gccgttgctg tacttctata
    87361 ccgaggaaaa atcccagcac tgccaggcta atacacagca gcaacatgct ctgttgggta
    87421 ttagggataa tcaccaaaaa gtagagcagc cctaacggca gcgcggccag cgtcccgtag
    87481 ataaagtcga tcgccaccat gcgtggattc ggcaaacgca ttgccagtga cgtcactacc
    87541 gcaatcatca ccattgcacc actgccggaa gtccagcccg tccacagcca gaaaagcgtg
    87601 cccagaatgc aggaaagtgt ggttcgccag aagttaacca tcgcatgatg acgttcggct
    87661 gactctactt tgacttccgg ttcgccttgc aggatctctt cttcggtggc gttgattttt
    87721 gtgttactga taacgccgcg cttgagaagc tgataacgcg ttgccgccgc gacccagcta
    87781 taaatggtga caggcgtttc ccgttccccg gtccaggcga taactctccg caggcgtttg
    87841 agctgcttgt ggacgtcctg cgcggtttct accggcgtgt caaaaaattc gcggaaagta
    87901 tcagtgatca attccgggcg cgtattctga ataagataag tttcgcagga ttgggtaatc
    87961 agcgtcagcg atagcgtatt gatcgctttt aaacgtcgat tggcccgcgc ccagcgggaa
    88021 gattccatat tcaggttgct gcgcatgcct tgtagcgccg tggtgcgtcg caccaggtcg
    88081 ccccaggctt tatcgacaac ttcaccatcg ccatgcttga tacagagttg cattaattga
    88141 tattgcgcga ccagcaaact ttccagctct cgatccactt cttgtttgat cgatcgcgga
    88201 gaaaagagca aatccgctat aatcgcacac acaataccga taacgatctc gctacaacgt
    88261 tcgacggcaa actgcggcgt aagcaatggt tccggctgaa tggtgatcac aatgatcagc
    88321 gcggtataac cggccagccc ccacgcgtac gagttttcta ttcgtaccag cgaggatatc
    88381 caggtacaaa aaccggccca gatacagcac accagaatca tcaataatgg tgcgcggatc
    88441 atcgcaatga tgatcaccag tccggcaata cagccaataa atgtgccgat gatgcgcaaa
    88501 aagccacgat aacgaatagc gccagaatac ggttcacctc ccgcagcaaa ggccggaccg
    88561 gcggcaacaa tcgccgctgt cagtaccgcc cagcgtggcg tttccagctg gaagtgaaag
    88621 ccaacaaaca gcgccagtac aatggcggtc gccagtttta ccgcaaagcg aatatgttgg
    88681 ttagcaatgg agaaaatacc catcgtgatt aaccaaactc acgcaggcga tgggccattt
    88741 tacggaagaa cgaatcctgg ctttcgtcgc gatcttgttt gccagtgacc accactgtag
    88801 cagtggtgcc cgcaggccag atgttctctt gctggttgtc gagacgaata cgaaccggaa
    88861 cacgttgcgc aagacgcacc cattcaaggt tagagtctat agtcgccatc cctttgtcgt
    88921 cacgcgtgct gctggcgttg gtgacccctg cggcaacact atcaacagtc cctttcagca
    88981 ctttgttact gccaagcggc gtgatctctg cacgataccc cggacgcacc ccttccagct
    89041 tagtttcttc catataggcc agtacataga aggagttctg tttcaccagc gcaaccgccg
    89101 ttgatcctcg agtaataaac tcaccggtat agacgttgag gttggtcacc cagccatctg
    89161 ctggcgcgcg gatcaccgtg cgttcaagat ccagttttgc cagatcgcgg gtcgcctgcg
    89221 ctttcgctaa ctgatgcaga accgtttgta gtac
//
LOCUS       DAJVOY010000002        80987 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVOY010000002 DAJVOY010000000
VERSION     DAJVOY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 80987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.002"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            561..1763
                     /gene="proV"
                     /locus_tag="OQL90_000094"
     CDS             561..1763
                     /gene="proV"
                     /locus_tag="OQL90_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1367007.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1756..2820
                     /gene="proW"
                     /locus_tag="OQL90_000095"
     CDS             1756..2820
                     /gene="proW"
                     /locus_tag="OQL90_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1367008.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQVLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2878..3870
                     /gene="proX"
                     /locus_tag="OQL90_000096"
     CDS             2878..3870
                     /gene="proX"
                     /locus_tag="OQL90_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1367009.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAALK"
     gene            4162..5346
                     /locus_tag="OQL90_000097"
     CDS             4162..5346
                     /locus_tag="OQL90_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1367010.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRPRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5470..6207
                     /gene="ygaZ"
                     /locus_tag="OQL90_000098"
     CDS             5470..6207
                     /gene="ygaZ"
                     /locus_tag="OQL90_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1367011.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6197..6532
                     /gene="ygaH"
                     /locus_tag="OQL90_000099"
     CDS             6197..6532
                     /gene="ygaH"
                     /locus_tag="OQL90_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1367012.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6623..7153
                     /gene="emrR"
                     /locus_tag="OQL90_000100"
     CDS             6623..7153
                     /gene="emrR"
                     /locus_tag="OQL90_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1367013.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7280..8452
                     /gene="emrA"
                     /locus_tag="OQL90_000101"
     CDS             7280..8452
                     /gene="emrA"
                     /locus_tag="OQL90_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCS1367014.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTVREISLAPVNKLIDDIVKANAG"
     gene            8469..10007
                     /gene="emrB"
                     /locus_tag="OQL90_000102"
     CDS             8469..10007
                     /gene="emrB"
                     /locus_tag="OQL90_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1367015.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(10071..10586)
                     /gene="luxS"
                     /locus_tag="OQL90_000103"
     CDS             complement(10071..10586)
                     /gene="luxS"
                     /locus_tag="OQL90_000103"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1367016.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10736..12292)
                     /gene="gshA"
                     /locus_tag="OQL90_000104"
     CDS             complement(10736..12292)
                     /gene="gshA"
                     /locus_tag="OQL90_000104"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1367017.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(12365..12793)
                     /locus_tag="OQL90_000105"
     CDS             complement(12365..12793)
                     /locus_tag="OQL90_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1367018.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12790..13356)
                     /gene="yqaB"
                     /locus_tag="OQL90_000106"
     CDS             complement(12790..13356)
                     /gene="yqaB"
                     /locus_tag="OQL90_000106"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS1367019.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLAPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13504..13580)
                     /locus_tag="OQL90_000107"
     tRNA            complement(13504..13580)
                     /locus_tag="OQL90_000107"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13544..13546),aa:Arg,seq:acg)
     gene            complement(13779..13855)
                     /locus_tag="OQL90_000108"
     tRNA            complement(13779..13855)
                     /locus_tag="OQL90_000108"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13819..13821),aa:Arg,seq:acg)
     gene            complement(14053..14129)
                     /locus_tag="OQL90_000109"
     tRNA            complement(14053..14129)
                     /locus_tag="OQL90_000109"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14093..14095),aa:Arg,seq:acg)
     gene            complement(14133..14225)
                     /locus_tag="OQL90_000110"
     tRNA            complement(14133..14225)
                     /locus_tag="OQL90_000110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14189..14191),aa:Ser,seq:gct)
     gene            complement(14541..14726)
                     /gene="csrA"
                     /locus_tag="OQL90_000111"
     CDS             complement(14541..14726)
                     /gene="csrA"
                     /locus_tag="OQL90_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1367020.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14961..17591)
                     /gene="alaS"
                     /locus_tag="OQL90_000112"
     CDS             complement(14961..17591)
                     /gene="alaS"
                     /locus_tag="OQL90_000112"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1367021.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEVAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17719..18219)
                     /gene="recX"
                     /locus_tag="OQL90_000113"
     CDS             complement(17719..18219)
                     /gene="recX"
                     /locus_tag="OQL90_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1367022.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18462..19523)
                     /gene="recA"
                     /locus_tag="OQL90_000114"
     CDS             complement(18462..19523)
                     /gene="recA"
                     /locus_tag="OQL90_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1367023.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19603..20100)
                     /gene="pncC"
                     /locus_tag="OQL90_000115"
     CDS             complement(19603..20100)
                     /gene="pncC"
                     /locus_tag="OQL90_000115"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1367024.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(20245..21330)
                     /gene="mltB"
                     /locus_tag="OQL90_000116"
     CDS             complement(20245..21330)
                     /gene="mltB"
                     /locus_tag="OQL90_000116"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1367025.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21586..22149
                     /gene="srlA"
                     /locus_tag="OQL90_000117"
                     /gene_synonym="gutA"
     CDS             21586..22149
                     /gene="srlA"
                     /locus_tag="OQL90_000117"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCS1367026.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLALSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            22146..23105
                     /gene="srlE"
                     /locus_tag="OQL90_000118"
                     /gene_synonym="gutE"
     CDS             22146..23105
                     /gene="srlE"
                     /locus_tag="OQL90_000118"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1367027.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            23116..23487
                     /gene="srlB"
                     /locus_tag="OQL90_000119"
                     /gene_synonym="gutB"
     CDS             23116..23487
                     /gene="srlB"
                     /locus_tag="OQL90_000119"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1367028.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23491..24270
                     /gene="srlD"
                     /locus_tag="OQL90_000120"
     CDS             23491..24270
                     /gene="srlD"
                     /locus_tag="OQL90_000120"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCS1367029.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24375..24733
                     /gene="gutM"
                     /locus_tag="OQL90_000121"
                     /pseudo
     CDS             24375..24733
                     /gene="gutM"
                     /locus_tag="OQL90_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            24800..25573
                     /gene="srlR"
                     /locus_tag="OQL90_000122"
                     /gene_synonym="gutR"
     CDS             24800..25573
                     /gene="srlR"
                     /locus_tag="OQL90_000122"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1367030.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25566..26531
                     /gene="gutQ"
                     /locus_tag="OQL90_000123"
     CDS             25566..26531
                     /gene="gutQ"
                     /locus_tag="OQL90_000123"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1367031.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26528..28042)
                     /gene="norR"
                     /locus_tag="OQL90_000124"
     CDS             complement(26528..28042)
                     /gene="norR"
                     /locus_tag="OQL90_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1367032.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            28229..29668
                     /gene="norV"
                     /locus_tag="OQL90_000125"
     CDS             28229..29668
                     /gene="norV"
                     /locus_tag="OQL90_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1367033.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29665..30798
                     /gene="norW"
                     /locus_tag="OQL90_000126"
                     /pseudo
     CDS             29665..30798
                     /gene="norW"
                     /locus_tag="OQL90_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(30926..33178)
                     /gene="hypF"
                     /locus_tag="OQL90_000127"
     CDS             complement(30926..33178)
                     /gene="hypF"
                     /locus_tag="OQL90_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1367034.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33331..33858)
                     /gene="hydN"
                     /locus_tag="OQL90_000128"
     CDS             complement(33331..33858)
                     /gene="hydN"
                     /locus_tag="OQL90_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1367035.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(34007..35017)
                     /gene="ascG"
                     /locus_tag="OQL90_000129"
     CDS             complement(34007..35017)
                     /gene="ascG"
                     /locus_tag="OQL90_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1367036.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            35277..36734
                     /gene="ascF"
                     /locus_tag="OQL90_000130"
     CDS             35277..36734
                     /gene="ascF"
                     /locus_tag="OQL90_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCS1367037.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            36743..38167
                     /gene="ascB"
                     /locus_tag="OQL90_000131"
     CDS             36743..38167
                     /gene="ascB"
                     /locus_tag="OQL90_000131"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCS1367038.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38326..38796)
                     /gene="hycI"
                     /locus_tag="OQL90_000132"
     CDS             complement(38326..38796)
                     /gene="hycI"
                     /locus_tag="OQL90_000132"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCS1367039.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38789..39199)
                     /gene="hycH"
                     /locus_tag="OQL90_000133"
     CDS             complement(38789..39199)
                     /gene="hycH"
                     /locus_tag="OQL90_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCS1367040.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(39196..39963)
                     /gene="hycG"
                     /locus_tag="OQL90_000134"
     CDS             complement(39196..39963)
                     /gene="hycG"
                     /locus_tag="OQL90_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCS1367041.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(39963..40505)
                     /gene="hycF"
                     /locus_tag="OQL90_000135"
     CDS             complement(39963..40505)
                     /gene="hycF"
                     /locus_tag="OQL90_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCS1367042.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(40515..42224)
                     /gene="hycE"
                     /locus_tag="OQL90_000136"
     CDS             complement(40515..42224)
                     /gene="hycE"
                     /locus_tag="OQL90_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1367043.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKEQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(42242..43165)
                     /gene="hycD"
                     /locus_tag="OQL90_000137"
     CDS             complement(42242..43165)
                     /gene="hycD"
                     /locus_tag="OQL90_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1367044.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITSRITWAGFGFAFLAFVSLLAA"
     gene            complement(43168..44994)
                     /gene="hycC"
                     /locus_tag="OQL90_000138"
     CDS             complement(43168..44994)
                     /gene="hycC"
                     /locus_tag="OQL90_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS1367045.1"
                     /translation="MSAISLINSGVVWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILILSLLGGNAPLWWGIVLLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(44991..45602)
                     /gene="hycB"
                     /locus_tag="OQL90_000139"
     CDS             complement(44991..45602)
                     /gene="hycB"
                     /locus_tag="OQL90_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS1367046.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNVITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(45727..46188)
                     /gene="hycA"
                     /locus_tag="OQL90_000140"
     CDS             complement(45727..46188)
                     /gene="hycA"
                     /locus_tag="OQL90_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS1367047.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            46400..46750
                     /gene="hypA"
                     /locus_tag="OQL90_000141"
     CDS             46400..46750
                     /gene="hypA"
                     /locus_tag="OQL90_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1367048.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            46754..47626
                     /gene="hypB"
                     /locus_tag="OQL90_000142"
     CDS             46754..47626
                     /gene="hypB"
                     /locus_tag="OQL90_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1367049.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            47617..47889
                     /gene="hypC"
                     /locus_tag="OQL90_000143"
     CDS             47617..47889
                     /gene="hypC"
                     /locus_tag="OQL90_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1367050.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            47889..49010
                     /gene="hypD"
                     /locus_tag="OQL90_000144"
     CDS             47889..49010
                     /gene="hypD"
                     /locus_tag="OQL90_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1367051.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            49007..50017
                     /gene="hypE"
                     /locus_tag="OQL90_000145"
     CDS             49007..50017
                     /gene="hypE"
                     /locus_tag="OQL90_000145"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1367052.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            50091..52169
                     /gene="flhA"
                     /locus_tag="OQL90_000146"
                     /pseudo
     CDS             50091..52169
                     /gene="flhA"
                     /locus_tag="OQL90_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(52206..52559)
                     /locus_tag="OQL90_000147"
     CDS             complement(52206..52559)
                     /locus_tag="OQL90_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1367053.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(52636..52770)
                     /locus_tag="OQL90_000148"
     CDS             complement(52636..52770)
                     /locus_tag="OQL90_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367054.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            52846..55407
                     /gene="mutS"
                     /locus_tag="OQL90_000149"
     CDS             52846..55407
                     /gene="mutS"
                     /locus_tag="OQL90_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1367055.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            55513..56169
                     /gene="pphB"
                     /locus_tag="OQL90_000150"
     CDS             55513..56169
                     /gene="pphB"
                     /locus_tag="OQL90_000150"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCS1367056.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(56220..56987)
                     /locus_tag="OQL90_000151"
     CDS             complement(56220..56987)
                     /locus_tag="OQL90_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1367057.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            57183..58091
                     /locus_tag="OQL90_000152"
     CDS             57183..58091
                     /locus_tag="OQL90_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1367058.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            58088..59350
                     /locus_tag="OQL90_000153"
     CDS             58088..59350
                     /locus_tag="OQL90_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCS1367059.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            59347..59985
                     /locus_tag="OQL90_000154"
     CDS             59347..59985
                     /locus_tag="OQL90_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1367060.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTVLSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            59990..60765
                     /locus_tag="OQL90_000155"
                     /pseudo
     CDS             59990..60765
                     /locus_tag="OQL90_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
     gene            60854..62218
                     /locus_tag="OQL90_000156"
     CDS             60854..62218
                     /locus_tag="OQL90_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCS1367061.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(62312..63304)
                     /gene="rpoS"
                     /locus_tag="OQL90_000157"
     CDS             complement(62312..63304)
                     /gene="rpoS"
                     /locus_tag="OQL90_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1367062.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(63367..64458)
                     /gene="nlpD"
                     /locus_tag="OQL90_000158"
     CDS             complement(63367..64458)
                     /gene="nlpD"
                     /locus_tag="OQL90_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1367063.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(64598..65224)
                     /gene="pcm"
                     /locus_tag="OQL90_000159"
     CDS             complement(64598..65224)
                     /gene="pcm"
                     /locus_tag="OQL90_000159"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1367064.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(65218..65979)
                     /gene="surE"
                     /locus_tag="OQL90_000160"
     CDS             complement(65218..65979)
                     /gene="surE"
                     /locus_tag="OQL90_000160"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1367065.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(65960..67009)
                     /gene="truD"
                     /locus_tag="OQL90_000161"
     CDS             complement(65960..67009)
                     /gene="truD"
                     /locus_tag="OQL90_000161"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1367066.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(67006..67485)
                     /gene="ispF"
                     /locus_tag="OQL90_000162"
     CDS             complement(67006..67485)
                     /gene="ispF"
                     /locus_tag="OQL90_000162"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1367067.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(67485..68195)
                     /gene="ispD"
                     /locus_tag="OQL90_000163"
     CDS             complement(67485..68195)
                     /gene="ispD"
                     /locus_tag="OQL90_000163"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1367068.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(68214..68525)
                     /gene="ftsB"
                     /locus_tag="OQL90_000164"
     CDS             complement(68214..68525)
                     /gene="ftsB"
                     /locus_tag="OQL90_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1367069.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(68719..69042)
                     /locus_tag="OQL90_000165"
     CDS             complement(68719..69042)
                     /locus_tag="OQL90_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1367070.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(69092..69697)
                     /gene="cysC"
                     /locus_tag="OQL90_000166"
     CDS             complement(69092..69697)
                     /gene="cysC"
                     /locus_tag="OQL90_000166"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1367071.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(69697..71124)
                     /gene="cysN"
                     /locus_tag="OQL90_000167"
     CDS             complement(69697..71124)
                     /gene="cysN"
                     /locus_tag="OQL90_000167"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1367072.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(71126..72034)
                     /gene="cysD"
                     /locus_tag="OQL90_000168"
     CDS             complement(71126..72034)
                     /gene="cysD"
                     /locus_tag="OQL90_000168"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1367073.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            72286..73323
                     /gene="iap"
                     /locus_tag="OQL90_000169"
     CDS             72286..73323
                     /gene="iap"
                     /locus_tag="OQL90_000169"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1367074.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTTEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(73530..73823)
                     /gene="cas2"
                     /locus_tag="OQL90_000170"
     CDS             complement(73530..73823)
                     /gene="cas2"
                     /locus_tag="OQL90_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCS1367075.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(73823..74743)
                     /gene="cas1e"
                     /locus_tag="OQL90_000171"
     CDS             complement(73823..74743)
                     /gene="cas1e"
                     /locus_tag="OQL90_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCS1367076.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(74740..75390)
                     /gene="cas6e"
                     /locus_tag="OQL90_000172"
     CDS             complement(74740..75390)
                     /gene="cas6e"
                     /locus_tag="OQL90_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS1367077.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(75372..76118)
                     /gene="cas5e"
                     /locus_tag="OQL90_000173"
     CDS             complement(75372..76118)
                     /gene="cas5e"
                     /locus_tag="OQL90_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCS1367078.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(76129..77207)
                     /gene="cas7e"
                     /locus_tag="OQL90_000174"
                     /pseudo
     CDS             complement(76129..77207)
                     /gene="cas7e"
                     /locus_tag="OQL90_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            complement(77222..77776)
                     /gene="casB"
                     /locus_tag="OQL90_000175"
     CDS             complement(77222..77776)
                     /gene="casB"
                     /locus_tag="OQL90_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCS1367079.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLEEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALGAGRWALEYYQAGDADNEQN"
     gene            complement(77773..79335)
                     /gene="casA"
                     /locus_tag="OQL90_000176"
     CDS             complement(77773..79335)
                     /gene="casA"
                     /locus_tag="OQL90_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCS1367080.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(79433..80987)
                     /gene="cas3"
                     /locus_tag="OQL90_000177"
                     /pseudo
     CDS             complement(79433..80987)
                     /gene="cas3"
                     /locus_tag="OQL90_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT        19101 a        21993 c        20701 g        19192 t
ORIGIN      
        1 ggatctatac ttttgcgggt caacataaga ggatgagtcc ttaataaaac aggtcctcag
       61 agcatacata ttctgcggag cgtgcggcaa caacaggacg aacaggggcg gttttcaatt
      121 tgcagccgtc aggctgccgt ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
      181 cagaagatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc
      241 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac
      301 tgagactctg ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta
      361 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg ggaaattccg
      421 gcgatttgct cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc
      481 agtatgttag ggtagaaaaa agtgactatt tccattgggt aatatatcga cataaacaaa
      541 taaaggaatc tttctattgc atggcaatta aattagaaat taaaaatctt tataaaatat
      601 ttggcgagca tccacagcga gcgttcaaat atatcgaaca aggactttcg aaagaacaaa
      661 ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag
      721 gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc
      781 tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
      841 aaatatccga tgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt
      901 cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg
      961 ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc
     1021 tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg
     1081 gattagcccg cgcgttagcg attaatccgg atatattatt aatggacgaa gccttctcgg
     1141 cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac
     1201 atcagcgcac cattgtcttt atttcccacg atctcgatga agctatgcgt attggcgacc
     1261 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca
     1321 ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtat
     1381 tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa acccctggct
     1441 tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta
     1501 tcgaacgcgg taataagttt gtcggcgcag tttccatcga ttcgcttaaa accgcgttaa
     1561 cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa
     1621 cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggta cccgtggtcg
     1681 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc
     1741 gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac
     1801 agtgccgtgc aatccgcaga cgcctggggt acaccggcga ctgcaccgac tgacggcggt
     1861 ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat
     1921 ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt
     1981 gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac
     2041 ggtttccagc aagtgctgct gggtatgccc gcaccggtgg cgattatcgt tttcgctctc
     2101 atcgcctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtttc gctgattgcc
     2161 atcggcgcaa tcggtgcctg gtcgcaggcc atggttaccc tggcgctggt gttaaccgcc
     2221 ctgctgttct gtatcgtcat tggtttgccg ctggggatct ggctggcgag aagtccgcga
     2281 gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat
     2341 ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc
     2401 atctttgctc tgccgccgat tatccgtctg accattctgg ggattaacca ggttccggcg
     2461 gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt
     2521 cagttaccgc tggcgatgcc gagcattatg gcgggcgtta accagacgct gatgctggcc
     2581 ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt
     2641 cgcggtatcg gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg gattgtgatc
     2701 ctcgccatta tcctcgatcg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc
     2761 aaccgtcgct ggtacaccac tggccctgtt ggtctgctga cccgcccatt cattaagtaa
     2821 ctctgcactt gcccggtgac gccgggcatt atcaccctgc caaaaaaagg aataacaatg
     2881 cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct
     2941 gccgatctgc cgggcaaagg tattactgtt aatccagttc aaagcaccat caccgaagaa
     3001 accttccaga cgctgctggt cagccgtgcg ctggagaaat taggctatac cgtcaacaaa
     3061 cccagcgaag tagattacaa cgttggctac accttgcttg cttccggcga tgcaaccttc
     3121 accgccgtga actggacgcc actgcatgac aacatgtacg aagctgccgg tggcgataag
     3181 aaattttatc gtgaaggggt atttgttaac ggcgcggcac agggttacct gatcgataag
     3241 aaaaccgccg accagtacaa aatcaccaac atcgcacaac tgaaagagcc gaagatcgcc
     3301 aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa tcctggctgg
     3361 ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg
     3421 cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc
     3481 aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg
     3541 aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat
     3601 accaaactgc cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc
     3661 aacaaagcct ggaccgagaa aaatccggcg gcagccaaac tgtttgccat tatgcagttg
     3721 ccagtggccg atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc
     3781 gatattcagg gccacgttga tggctggatc aaagcccacc agcagcagtt cgatggctgg
     3841 gtgaatgagg cgctggcagc gctgaagtaa tttttattca ggcggataag gcgtttttgc
     3901 cttatccgcc gctctgtaca caatgcctga tgcgacgctg acgcgtctta tcatgcctac
     3961 aaaatacact cattccgtat ggcggataag gcgttttcgc cgcatccgcc gttctgtgca
     4021 caatgcctga tgcgacgctg gcgcgtctta tcatgcctac aaaccacatc gcacatttca
     4081 acaagctatc tttcatccca tattcatcaa catccgctat tattgatttc cagcttaatc
     4141 atcacctgat gaacaaaaat aatgactaaa cctaatcatg agcttagccc ggcgctgatc
     4201 gtgctgatgt ctatcgccac cggtctggcg gtagccagta actattacgc ccagccattg
     4261 ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt cggcaggctt tattgttacc
     4321 gccgcgcaat tgggctatgc agcaggtctg ctgtttcttg ttcccctcgg tgatatgttt
     4381 gaacgccgcc gcctgattgt ctcgatgacc ttactggcgg caggcggcat gttaatcact
     4441 gccagcagtc agtcgctggc gatgatgatc ctcggtacgg cattaaccgg tttattctct
     4501 gtcgtggcac aaattctggt tccgctggca gcgacgctgg cttcaccgga caaacgcggc
     4561 aaagtggttg gcactattat gagcggtctg ctgttgggga tcttactcgc acggaccgtt
     4621 gccggattgc tggcgaatct cggcggctgg cgcaccgtct tttgggttgc ctcggtgtta
     4681 atggcactga tggcgctggc attatggcgt ggtctgccac aaatgaaatc agaaacccac
     4741 ctcaactacc cacagttgtt aggttccgtt ttcagcatgt ttatcagcga taagatcctg
     4801 cgcccccgcg cattgctggg ctgcctgacc tttgccaact tcagcattct ctggacctca
     4861 atggcctttt tgcttgccgc tccacctttt aactacagcg atggtgtcat tggtctgttt
     4921 ggacttgcgg gagctgccgg agcgttgggc gctcgtccgg cgggcggttt tgccgataag
     4981 ggtaaatcgc accacaccac aactttcggt ctgctgctgc tattactttc atggctggcg
     5041 atctggtttg gtcacacttc cgtactggcg ctgattatcg gcatcctggt gctggatctc
     5101 accgtacagg gcgtgcatat cactaaccag acggtaattt atcgaataca ccctgatgcg
     5161 cgtaatcgcc tgaccgcagg ttacatgacc agctacttta ttggcggtgc cgccggttcg
     5221 ctaatttcag cctcagcctg gcaacatggc ggttgggctg gcgtttgtct ggctggcgcg
     5281 acgattgccc tggttaactt actggtctgg tggcgaggtt ttcatcgtca ggaagccgca
     5341 aattaagcaa tcagcaaccg ttttcgtggc tttacactta taagggtgtt aagaagccca
     5401 tcagtctgat aaggttaaga tattcattca gtctatttat aatattaaca atcgttaagc
     5461 gtacactcta tggaaagccc tactccacag cctgctcttg gttcggcgac cttcatggaa
     5521 ggatgcaaag acagtttacc gattgttatt agttatattc cggtggcctt tgcgttcggt
     5581 ctgaatgcga cccgtctggg attctctcct ctcgaaagcg tttttttctc ctgcatcatt
     5641 tatgcaggcg cgagccagtt cgtcattacc gcgatgctgg cagccgggag tagtttgtgg
     5701 gttgctgcac tgaccgtcat ggcaatggat gttcgccatg tgttgtatgg cccgtcactg
     5761 cgtagccgta ttattcagcg tctgcaaaaa tcgaaaaccg ccctgtgggc atttggcctg
     5821 acggatgagg tttttgccgc cgcaaccgca aaactggtac gcaataatcg ccgctggagc
     5881 gagaactgga tgatcggcat tgccttcagt tcatggtcat cgtgggtatt tggtacggta
     5941 ataggggcat tctccggcag cggcttgctg caaggttatc ccgccgttga agctgcatta
     6001 ggttttatgc ttccggcact ctttatgagt ttcctgctcg cctctttcca gcgcaaacaa
     6061 tctctttgcg ttaccgcagc gttagttggt gcccttgcag gcgtaacgct attttctatt
     6121 cccgtcgcca ttctggcagg cattgtctgt ggctgcctca ctgcgttaat ccaggcattc
     6181 tggcaaggag cgcccgatga gctatgaggt tctgctgctt gggttactgg ttggcgtggc
     6241 gaattattgc ttccgctatt tgccgctgcg cctgcgtgtg ggtaatgccc gcccaaccaa
     6301 acgtggcgcg gtaggtattt tgctcgacac cattggcatc gcctcgatat gcgctctgct
     6361 ggttgtctct accgcaccag aagtgatgca cgatacacgc cgtttcgtgc ccacgctggt
     6421 cggcttcgcg gtactgggtg ccagtttcta taaaacacgc agcattatca tcccaacact
     6481 gcttagtgcg ctggcctatg ggctcgcctg gaaagtgatg gcgattatat aacccacaag
     6541 aatcattttt ctaaaacaat acatttactt tatttgttac tgtcgttact atatcggctg
     6601 aaattaatga ggtcataccc aaatggatag ttcgtttacg cccattgaac aaatgctaaa
     6661 atttcgcgcc agccgccacg aagattttcc ttatcaggag atccttctga ctcgtctttg
     6721 catgcacatg caaagcaagc tgctggagaa ccgcaataaa atgctgaagg ctcaggggat
     6781 taacgagacg ttgtttatgg cgttgattac gctggagtct caggaaaacc acagtattca
     6841 gccttctgaa ttaagttgtg ctcttggatc atcccgtacc aacgcgacgc gtattgccga
     6901 tgaactggaa aaacgcggtt ggatcgaacg tcgtgaaagc gataacgatc gccgctgcct
     6961 gcatctgcaa ttaacggaaa aaggtcacga gtttttgcgc gaggttttac caccgcagca
     7021 taactgcctg catcaactct ggtccgcgct cagcacaaca gaaaaagatc agctcgagca
     7081 aatcacccgc aaattgctct cccgtctcga ccagatggaa caagacggtg tggttctcga
     7141 agcgatgagc taacgcgtca tctcgctcaa aaatccagat ttataaaaga aaaaatgacc
     7201 ggccagcatc gcaacatgct ggcctttttg gcaagcaggt cggctcagcc gatgagttaa
     7261 gaagatcgtg gagaacaata tgagcgcaaa tgcggagact caaaccccgc agcaaccggt
     7321 aaagaagagc ggcaaacgta agcgtctgct cctccttctc accttgctct ttataattat
     7381 tgccgtagcg atagggattt attggttttt ggtactgcgt catttcgaag aaaccgatga
     7441 cgcatacgtg gcagggaatc agattcaaat tatgtctcag gtgtctggca gcgtgacgaa
     7501 agtctgggcc gataacaccg attttgtaaa agaaggcgat gttctggtca ctctcgaccc
     7561 gacagatgct cgccaggcgt ttgaaaaagc caaaactgca ctggcttcca gcgttcgcca
     7621 aacccaccag ctgatgatta acagcaaaca gttacaggcg aatattgagg tgcaaaaaat
     7681 cgccctcgcg aaagcacaaa gcgactacaa ccgccgtgtg ccgctgggca atgccaacct
     7741 gattggtcgc gaagagctgc aacacgcccg cgacgccgtc accagtgccc aggcgcaact
     7801 ggacgtcgct attcaacaat acaatgccaa tcaggcgatg attctgggga ctaaactgga
     7861 agatcagcct gccgtgcaac aggctgccac cgaagtacgt aacgcctggc tggcgctgga
     7921 gcgtacccgt attgtcagtc cgatgaccgg ttatgtctcc cgccgcgcgg tacagcctgg
     7981 ggcgcaaatt agcccaacga cgccgctgat ggcggtcgtt ccagccacca atatgtgggt
     8041 ggatgccaac tttaaagaga cgcagattgc caatatgcgt atcggtcagc cggtcactat
     8101 caccacggat atttacggcg atgatgtgaa atacaccggt aaagtggttg gtctggatat
     8161 gggcacaggt agcgcattct cactgcttcc ggcgcaaaat gcgaccggta actggatcaa
     8221 agtcgttcag cgtctacctg tgcgtatcga actggaccag aaacagctgg agcaataccc
     8281 gctgcgcatt ggtttgtcca cgctggtgag cgtcaatacc actaaccgtg acggtcaggt
     8341 actggcaaat aaagtacgtt caacaccggt cgcggtaagt accgtgcgtg aaatcagcct
     8401 ggcacctgtc aataaactga tcgacgatat cgtaaaagct aacgctggct aatccagagg
     8461 tgcgtgtgat gcaacagcaa aaaccgctgg aaggcgcgca actggtcatt atgacgattg
     8521 cgctgtcact ggcgacattc atgcaggtgc tggactccac cattgctaac gtggcgatcc
     8581 ccactatcgc cgggaatctg ggctcatcgc tcagccaggg aacgtgggta atcacttctt
     8641 tcggggtggc gaatgccatc tcgatcccgc ttaccggctg gctggcaaag cgcgtcgggg
     8701 aagtgaaact gttcctttgg tccaccatcg cctttgctat tgcgtcgtgg gagtgtggtg
     8761 tctccagcag cctgaatatg ctgatcttct tccgtgtgat tcaggggatt gtcgctgggc
     8821 cgttgatccc actttcgcaa agtcttttgc tgaataatta cccgccagcc aaacgctcaa
     8881 tcgcgctggc gttgtggtcg atgacggtga ttgtcgcgcc aatttgcggc ccaatcctcg
     8941 gcggttatat cagcgataat taccactggg gctggatatt cttcatcaac gtgccaattg
     9001 gcgtggcggt ggtgttgatg acactgcaaa ccctgcgcgg acgcgaaacc cgcaccgaac
     9061 ggcggcggat tgatgccgtg gggctggcac tgctggttat tggtatcggt agcctgcaga
     9121 ttatgctcga ccgcggtaaa gagctggact ggttttcatc acaggaaatt atcattctta
     9181 ccgtggtggc ggtggtggct atctgcttcc tgattgtctg ggagctgacc gacgataacc
     9241 cgatagtcga tctgtcgttg tttaagtcgc gcaacttcac catcggctgc ttgtgtatca
     9301 gcctcgcgta tatgctctac ttcggcgcga ttgttctgct gccgcagttg ttgcaggagg
     9361 tctacggtta cacggcgacc tgggcaggtt tggcctctgc gccggtaggg attattccgg
     9421 tgatcctgtc gccgattatc ggccgcttcg cgcataaact ggatatgcgg cggctggtaa
     9481 ccttcagctt tattatgtat gccgtctgct tctactggcg tgcctatacc tttgaaccag
     9541 gtatggattt tggcgcgtcg gcctggccgc agtttatcca ggggtttgcg gtggcctgct
     9601 tctttatgcc gctgaccacc attacgctgt ctggtttgcc accggaacga ctggcggcgg
     9661 catcgagcct ctctaacttt acgcgaacgc tggcggggtc tatcggcacg tcgataacca
     9721 cgaccatgtg gaccaaccgc gagtcgatgc accatgcgca gttgactgag tcggtaaacc
     9781 cgttcaaccc gaatgcccag gcgatgtaca gtcaactgga agggcttggg atgacgcaac
     9841 agcaggcatc aggctggatt gcccagcaga tcactaatca ggggctgatt atttccgcca
     9901 atgagatctt ctggatgtca gccgggatat tcctcgtcct gctggggctg gtgtggtttg
     9961 ctaaaccgcc ctttggcgca ggtggcggcg gaggtggtgc gcactaagta caactaagcc
    10021 agttcatttg aactggcttt tttcaattaa ttgtgaagat agtttactga ctaaatgtgc
    10081 agttcctgca acttctcttt cggcagtgcc agttcttcgt tgctgttgat gcgtacgtca
    10141 cgttccagaa tgctacgcgc aatatcctgc gcttcctgca acgagtgcat ctggtaagtg
    10201 ccacactggt agacgttcag ctccgggatc tgattctgat cctgcacttt cagcacgtct
    10261 tccattgccg ctttccaggc atcagcaaca cgctgctcat ctggcgtacc aatcagactc
    10321 atataaaaac cggtgcggca gcccattggc gagatatcga taatctctac accattaccg
    10381 ttaagatggt tacgcataaa accagcaaac aggtgctcca gagtatggct ccctctttct
    10441 ggcatcactt ctttgttcgg cacgcagaag cgcagatcga acacggtgat tgcgtcgcca
    10501 tgcggggtgt tcattgtttt cgccacccga actgcaggcg cttccatccg ggtatgatcg
    10561 actgtgaagc tatctaacaa cggcatttag ccacctccgg taattttttt aaaaattttc
    10621 tgaactcttt cttcccaggc gagtctgagt atatgaaaga cgcgcatttg ttatcatcat
    10681 ccctgaattc agagatgaaa ttttggccac tcacgagtgg cctttttctt ttctgtcagg
    10741 cgtgtttttc cagccacacc gcaaacggtt cggtatcagc ggtttccatt tcctgctgac
    10801 ggcgttcaga tgcctcgcgc tcggctacaa aatcctcttc gcgcagaatt tccaacggct
    10861 cttcacgcag cagattacgg taggcttctg caaacgcttt gcctgttccg ccaataccag
    10921 tatcaatcat agaccttaag atacgggcag agaaagtcag atcgggatta tcgaagcagg
    10981 cgaccagttc atcacacact ttctgatacg ctttgccgcc gttaatgcta tccaacgttt
    11041 gcgcgacgcg tttcagatcg cggaacagat ctttacccac ctgcggtaac gggaactgcg
    11101 cggtttcgca gccgataccc agcgtcaggc ccggtttgcg accttcgagg atcacccggt
    11161 tccagttaac gcgtgtacag gcaagttcgc tactgctcat ttccggtgca tcagccagcg
    11221 cacaccagac cataaacagg tcgaggaatc gcacctgctg ttcatctaca ccaatcggcg
    11281 agaacgggtt gatgtccagc gaacgcactt caatatattc aatgccgcca cgtaacagcg
    11341 catcagaagg tgactcgccg ctgcgggtaa cgcgttttgg acgaatcggc gcatacagtt
    11401 cgttttcaat ctgcagcacg ttgctattga tttgcagcct cttaccgtct ttctcaatac
    11461 caatcttcgc gtactcttcc gatggcgttt tgattgcctg tttaaggccc gctacgtact
    11521 cgtaaagatc gttgaaggta ataccaagat tgctttgcga tttattggta tagccgagat
    11581 cgctcaaacg aagagaggtc gcatacggca ggtaatacat accgcactcg gttttctcaa
    11641 acggcagcga cgttggtttt ccttgcagga aagaagaaca aatcgccgga gatgcaccaa
    11701 acagataagg aatgacccaa ccgaaacgat agtaattgcg gataacgcgg aaatagcccg
    11761 cagaaatttt ctctttggca tcagtgcccg agatatcacc gcacttcgct tgccagaatg
    11821 ccattggcaa agagaaattg tagtgcacgc cggaaatggt ttgcatcagc gcgccgtagc
    11881 gatttttcag cccttcacga tacagcgttt taaagcgtcc ggtgttagaa gtgccgtact
    11941 gtgccagttc gatgtcctga ccttctgcga tgtagcatgg catacttaac ggccacatcc
    12001 gctcatcgcc catattgcgc gccgtataac gatgcagatc gcgcataaag gtcaacatat
    12061 gttcaatatc accatccact ggtgtaatga attccagcaa tgcttccgca aaatcggtag
    12121 taatccattt gtgcgtcagt gcggaaccta atgcttcagg atgacctgtt gttgcctgtg
    12181 tgccatcagc attaacacgc aaagtttcgc gctccagccc acgctgtatc ccctttaacg
    12241 cctgaggatg tttttccagc caggccagcg cctgtgatac gtccgggatc aaattgacct
    12301 cccgcctgtc aaaatcgttt taattagcat aactgtaatg gtgaccatat gtgcaggcct
    12361 acaattagtg ccaccacatc atgccctgaa cggtagctgc tgcaactgca acatagcgta
    12421 aagctttacc aaggcacaaa aaaaagatta ccggtcccca cgagatgcgc atccatcccg
    12481 ctaacagaca cagtaaatcg ccaaccacgg gcatccagct taataatagc gtgactgcac
    12541 catagcgttt cagccagcca gtagctttct cttgccagcg cgatgtttta cgcaatggaa
    12601 agaaacgccc aaggataacg ttagttaacc ctccaaggct attacccatt gttgctgtta
    12661 aaactaaaac ccagggataa ctgatcccgg aaagcaacat tgccaccagc acgacttcgg
    12721 agttgccggg taatagtgta gcgctgagaa aactactggc aaacaacgag aaaagcgata
    12781 acgcttcact cacagcaagc gaacatccac ggcgtccatg cctgctgcac gggccgcctg
    12841 aataccgaaa tcggcatctt caaagaccac acactgcgtc ggttgcacgc ccatacgctg
    12901 cgcgcacaac aaaaatgtgt ctggcgcggg tttatggtgt ttgacgtgat cagcagcgac
    12961 gacggcgtca aaataacggc gtaatcccag gtgcgccagc aatgcctcag cgatggcgct
    13021 ttcactcccc gttcctacag ccattgggcg acgaccatgc caacttttca ccacttcaac
    13081 aagaggaagc ggctcgacac tatccagcag catacttctt actgcttctg ttttttcacg
    13141 cgctaacgca tgcggggcga gatcggcctg attcagctca ataattgcct gagcaatacg
    13201 ccaggtgggc gatccattaa gcgcaatcat tgcctgaaca tcgtactgaa gaccgtagtg
    13261 ccctaatact tcgcgccacg ctttacggtg cgtaggctcc gtatccagga ttgtgccatc
    13321 catatcaaaa attaaacctg cataacgctc gtacatggtc ttctcgcaag tcgaaaaata
    13381 tgacgctact ttagcgtaat tgcttgattt tgtcgctgat ggcgaaggag aagaaaattc
    13441 aggaaatgga taaagtagtt atttagaaat gagatatttt tgaaggaaat ttttgggaga
    13501 agatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta
    13561 tccagctgag ctacggatgc atcgggaaaa tttattttac tgctgatatt gatatcgcta
    13621 ctaaagccat atcaagtaag agatggtgca tccgggagga ttactcgcct tgcggctcgc
    13681 ccttcgggcc gttgctaaag caacgttatc atccctagtg cctgcggtta attccgctac
    13741 ctcttcgata ccttcattgc tgaagatttc ggagaatatg gtgcatccgg gaggattcga
    13801 acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg
    13861 gaaacttact ttactgcaga ttttttgata ccgctactaa agccgtatca agtaagagat
    13921 ggtgcattcg ggaggattac tcgccttgcg gctcgccctt ctggccgttg ctaaagcaac
    13981 gttatcctcc ctggtgcctg cggttatacc gccacctctt ccataccttc atcgctgaaa
    14041 gtacgtagaa tatggtgcat ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg
    14101 agtactctat ccagctgagc tacggatgca aatggcggtg aggcggggat tcgaaccccg
    14161 gatgcagctt ttgaccgcat actcccttag caggggagcg ccttcagcct ctcggccacc
    14221 tcaccacacg cctcttacga gtgcttcgaa gaacttgttt atcgctcatc gtcgctgcgt
    14281 ggcgcacata ttactttctg agacttataa gtcaaacaat ttttcccaca cttttatcgt
    14341 ttgcacactt cacgctcaat tagtctgtaa aaacgtcaaa aagagtgttt tatctacaga
    14401 aaaacggagg tctggcagat agcagtaatg caaaaaaatg gagacttaag ttgaatgaac
    14461 gggagtaaag cgaaaagact atagagtgaa ggagaaattt tgagggtgcg tctcaccgat
    14521 aaagatgaga cgcggaaaga ttagtaactg gactgctggg atttttcagc ctggatacgc
    14581 tggtagatct cttcacggtg aacagaaact tccttcgggg catttacgcc aatacgtacc
    14641 tggttgccct tcacccctaa aactgtcacg gtgacctcat ccccaatcat gagggtctca
    14701 ccaactcgac gagtcagaat cagcattctt tgctccttga aagattaaaa gagtcgggtc
    14761 tctctgtatc ccggcattat ccatcatata acgccaaaaa gtaagcaatg acaaacacat
    14821 tacatgtaag cagtcatggc attacattct gttaaaccta agtttagccg atatacacaa
    14881 cttcaacctg actttatcgt tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac
    14941 ggcattgcct gacgcttata ttattgcaat ttcgcgctga cccagccttt cacactggct
    15001 aacgctgcag gtaaggccgc agcatccgta ccaccggctt gcgccatgtc aggacgtcca
    15061 ccacccttgc cgcccacctg ctgagcgacc ataccaatca gttcccctgc tttcacacga
    15121 tctgtgacgt ccttagatac gcctgcaatc agagaaacct taccttcggc taccgttgcc
    15181 agcacgataa ttgtcgaccc cagctgattt tttaaatcgt caaccatggt acgcaacatt
    15241 ttcggctcaa caccgctaag ctcgctaacc aacagcttaa caccattaac atcaattgcc
    15301 ttactggaaa gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct
    15361 ttttctaact gacgcgtacg ttccagtact gagcgcactt tatccgccag attattgcta
    15421 tcgcctttca gcagatgcgc gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg
    15481 atagcgcctt ctccggttac cgcttcgata cgacgaacgc ctgcagcagt acccgattca
    15541 gagatgatgc ggaacagacc aatatcacca gtgcggctgg cgtgagtacc gccacacagc
    15601 tcggtggaga aatcgcccat gctcagcacg cgtacacgct catcatactt ctcgccgaac
    15661 agcgccatcg cacctttcgc tttcgccact tcgagatcca tgatgttggt ttcgatcggc
    15721 aaattgcgac gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc ttccggtttc
    15781 atcgcttcgt tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc
    15841 tgcgatacat gagtccccag aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag
    15901 tgattcagac gaatacgggc gcgacgagcc tcatcaacat cagcctgcac agcgtcgccc
    15961 actttcagag aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc
    16021 gtatcttcca ccgcgaagga gaagttagcg tctttcagtt cgcctttatc accaacctga
    16081 ccgccggatt ccgcatagaa tggtgtttga tctagcacga ccacagcttc ctggcctgca
    16141 ttgatggcat caaccgcttt accatcaaca aacagcgcgg tcactttgcc gttcagttcc
    16201 agatggtcat agcctttaaa ttcagatgca ctatcaacac ggatcattgc gttgtaatcg
    16261 gcaccaaagc cgctggcttc gcgcgcgcga cgacgctgct cttccattgc tgcttcaaaa
    16321 ccagcttcgt caactttgat gttgcgctca cgacaaacat cagccgtcag gtcaaccggg
    16381 aagccatagg tgtcgtacag acggaaagcc gtttcaccat ccagcgtatc accagaaagt
    16441 tttgccagct cttcatccag caacgccaga ccgcgctcca gagtacgggc aaactgctct
    16501 tcttcagtct tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca
    16561 gagcccataa cgtcgatcag cggaccaacc agtttgtaga agaaggtttc tttcgcgccg
    16621 agcatattgc cgtgacgcac tgcgcgacga atgatacgac gcagtacata accacggttt
    16681 tcattggacg gcattacgcc atccgcgatc aggaacgcac aagaacgaat gtggtcagcg
    16741 attacgcgca gcgatttatt gctcagatcg gtcgcgccag tgactttcgc taccgcctgg
    16801 atcaacgtgc ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag caccgcagca
    16861 atacgctcca gacccatacc ggtatctaca gacggcttcg gcagcggttc catcgtgcca
    16921 tctgcctggc ggttgaactg catgaagacg atgttccaga tctcaatgta gcggtcgccg
    16981 tcttcttccg gacttcccgg agggccaccc caaatgtggt cgccgtgatc gtagaagatt
    17041 tcggtgcacg ggccgcacgg accagtgtca cccatctgcc agaagttgtc agatgcgtat
    17101 ggcgcacctt tgttatcgcc gatgcgaata atacgttcgc gcgggatccc gacttctttt
    17161 tcccagattt cgtaggcttc gtcgtcgctt tcatagacgg taacccacag acgctctttc
    17221 ggcagggcaa accatttttc gctggtcagc agttcccatg caaactgaat ggcatcgtgt
    17281 ttgaaatagt cgccgaagct gaagttgccc agcatttcga agaaggtatg gtgacgcgcg
    17341 gtgtaaccga cgttttccag gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa
    17401 gtggtagcgc gggaataatt acgcttgtcg agcccaagga acacatcctt gaactgattc
    17461 atcccggcgt tggtaaacaa caaagttggg tcgttatggg gtaccaggga gctgctggca
    17521 actacctgat gtcccttact atggaaaaag tcgagaaacg cctgacggat ctcagcggtg
    17581 ctcttgctca taattatcct gaaatcaagc taacgaaata tcgccaccag ctccagcgtg
    17641 tcttaaccgc cgggctggta actgaaaagt gggaataaga taagttttct tgactgggaa
    17701 gtaaaatacc gtatgcgttc agtcggcaaa atttcgccaa atatcctgga tatcttccat
    17761 cagatagcca cgatagagca gaaaacgctg gatcttaact ttttctgaaa agacagttgg
    17821 caaaggttca tcatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc
    17881 acattcacgc atcgcttttt ctgtcgcttc gcgggaaata cctttctgat tcagttcctg
    17941 acgaatacgt gcaggtccat aacctttgcg gctacggctg gcgataaagc gcgcaacaaa
    18001 tcggctgtca tcgagatagc catgttcatg gcaccatgca ataacgcgct cgtaatcttc
    18061 tgccgtagca tcaatctctt ctgggccatt tttgcccata atcggtgccg cgagtttacg
    18121 tcgcagttct tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac gatccaacag
    18181 gcgagcatat gccgggcgac gggacgttga ttctgtcatg gcatatcctt acaacttaaa
    18241 aaagcaaaag ggccgcagat gcgacccagg ttttgctgat aaacagattt aatagtcaat
    18301 gagtgtgcag aatgttcccg caggcccaat tcgttagagg atcgaggtga gagtaatttt
    18361 tcacgccagt gctggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac
    18421 cgtccgtgca cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt
    18481 tctgctacgc cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc
    18541 agcaactcac gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag
    18601 gcagttgcat tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct
    18661 gctttctcga tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata
    18721 ccttcgccgt agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc
    18781 accactttca cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata
    18841 cgacggatgt cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt
    18901 tccgggttac cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc
    18961 gtgttggact gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc
    19021 gcaaggccca tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc
    19081 gccacggagt caacgacgat aacgtctact gcaccagaac gcgccagggc gtcacagatt
    19141 tccagtgcct gctcgccggt gtccggctgg gagcacagca ggttgtcgat atcgacgccc
    19201 agtttacgtg cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta
    19261 ccttcacgct gcgctgcggc gatcacctgc agcgtcagcg tggttttacc ggaagattcc
    19321 ggtccgtaga tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc
    19381 agtgaaagcg aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc
    19441 atgatggagc ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc
    19501 tgtttgtttt cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca
    19561 atatgttctg ttgaagcaat tatactgtat gctcatacag tatcaagtgt tttgtagaaa
    19621 ttgttgccac aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc
    19681 gccgctgaag cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca
    19741 gacggtgccg acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga
    19801 cacggcataa tcagcacgag ccgctttcag tgcgcctatc accatttcca ccacgacggg
    19861 ttcactcacc gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt
    19921 ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc
    19981 ggtaatcact ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc
    20041 acgggctttc agcgcctgcc caacctgttc acttaactgc atcagttcac tgtcagtcat
    20101 cacaacctct gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg
    20161 gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag
    20221 ctcacgcccc cctcttgcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa
    20281 ctgccagacc gccattgcgt aatgggtgct gtggttataa cgggtaatgg tgtagaagtt
    20341 cggcagaccg taccagtact ggtaaccggt accgacatcc agacgcagca ggctggcttg
    20401 ttgatggttg cccagcggct gctgtggcgt taaacctgcc gtggcaagct gcgaaatgct
    20461 gtacctggtt ttgaagccat ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac
    20521 ctgatcgcct ttcacccagc cgtgcgcttt gaaatagttc gccacgctac cgatcgcatc
    20581 aaccggatcc cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta
    20641 agacgacggc ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag
    20701 cggatcgtcc tgctcgtcgc gcgccatcag caggaaggtt tccagctcgc cagagaaata
    20761 ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt
    20821 ccccatcacg cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg
    20881 tacgccatac acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac
    20941 accgttctgc acgttgtccg gcgtaataaa ttttttgcga taacgcagcc atgcgccgtt
    21001 cggacctgat ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac
    21061 cgaatccaga cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg
    21121 tttgttcacc attttgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc
    21181 catctgcatc acattgtgct gcggctcaag caggaagccg ccagacggcg ttccggtggt
    21241 cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg
    21301 aagcaatgtt acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg
    21361 gcaagtatgg taaagcatca cgccccgcac aaggaagcgg tagtcactgc ccgatacgga
    21421 ctttacataa ctcaactcat tcccctcgct atccttttac tcaaacttcc aaattaaaat
    21481 atttatcttt catttttaga ttaaaataac acttttaaat ctttcaatct gattagatta
    21541 ggttgccgtt tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt
    21601 actcatggtg cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg
    21661 atggtgaccg gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt
    21721 aattttatcg gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt
    21781 tcccgttacc tgctgctacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta
    21841 agccagggtc gctttatgcc ggaaaagtac aaacccagct actacgcggc ggcctcttat
    21901 agctgccact caatgaatgg cctctttccc catatcaacc ctggcgaact gtttgtttat
    21961 cttggcattg ccagcggtct gacaacgctg aacctgccac ttggcccact ggcgttgagc
    22021 tatctgctgg ttggtctggt caccaacttc tttcgcggct gggtgaccga tctgaccacc
    22081 gccatttttg agaaaaagat gggcattcaa cttgaacaaa aagttcacct ggcaggagca
    22141 acatcatgac gcgtattcgg atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc
    22201 tggaagccac gccgggcaaa aaaatcgtct atatcaccgc cggtacccgg cctgcgattg
    22261 ttgacaaact ggcacaactt actggctggc aggctattga cggatttaaa gaaggtgaac
    22321 ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca
    22381 tctatccgaa acgacgtatt cccaccatta atatccactc gacgggcaag tccggtccgc
    22441 tggcgcagta cattgtggaa gatatttatg tctctggcgt aaaagaagaa aacatcactg
    22501 tagtgggtga tgcgacacca caaccctctt ccgtgggccg tgactatgac accagtaaga
    22561 aaatcaccga acaaagcgat ggtttactgg cgaaggtggg aatgggcatg gggtccgccg
    22621 ttgcggtgct gtttcaatct ggtcgtgaca ccatcgacac tgtattaaaa accattctgc
    22681 cgtttatggc attcgtctcg gcgctcattg gcatcattat ggcttctggc cttggtgact
    22741 ggattgccca cggtcttgct ccgctggcga gccatccact gggtctggtc atgctggcgc
    22801 tcatctgctc cttcccactg ctttcacctt tcctcggccc aggcgcagtt atcgcacagg
    22861 ttatcggcgt attgattggc gtgcagattg gtctcggcaa tattccgccg catctggctt
    22921 taccggcact gtttgccatc aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt
    22981 cgctggcgga agcccgtcag gacacggttc gcgtcggtgt cccttctgta ctggtgagcc
    23041 gctttttaac cggcgcaccg actgtactga tcgcctggtt tgtctccggt tttatctatc
    23101 aatagaggct gaaacatgac cgttatttat aagaccacca tcacccgtat cggcgcgagt
    23161 gccactgacg ccctcagcga ccagatgctc atcacctttc gtgaaggcgc gccagcggac
    23221 ctcgaagagt attgcttcat tcattgccac ggcgagttga aaggtgcact ccatcccggt
    23281 ttgcaattta cgctcgggca gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa
    23341 gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa
    23401 tttccgggca ctgtccatgt ggcaggccct gtacccgacg atatcgcgcc gggatcggtt
    23461 ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt
    23521 gggcaaacct taggcgcgtt cctgtgccac ggtctggttg ccgaggggta tcgcgtcgcg
    23581 gttgtcgata ttcagagcga caaagccgca aatgtggcac aagaaattaa cgccgaatat
    23641 ggtgaaagta tggcgtacgg ttttggtgct gacgccacta gcgagcaaag cgttctggcg
    23701 ctctctcgtg gggtagatga aatctttggt cgcgtggatt tgctggtcta tagcgccgga
    23761 atagcaaaag cagcctttat cagcgacttc cagctcggcg attttgaccg ttcgctacaa
    23821 gtgaatctgg tgggttattt cctgtgtgcc cgtgaatttt cgcgtttgat gatccgcgac
    23881 gggattcagg gtcgcattat tcagatcaac tcgaaatccg gcaaagtggg cagcaaacac
    23941 aactctggct acagcgcagc gaaatttggt ggcgtcgggc tgactcaatc actggcgctg
    24001 gatctggcgg agtacggcat tacggtgcat tcactgatgc tcggtaacct gctgaaatcg
    24061 ccgatgttcc agtcactgtt gccacaatac gcgaccaagc tgggtatcaa accggatcaa
    24121 gtcgagcagt attacattga caaagtaccg ctcaaacgcg gctgcgatta tcaagatgtg
    24181 ctgaatatgc tgctgttcta cgccagtcct aaggcgtcgt actgcaccgg acagtcgatc
    24241 aatgtcaccg gcggtcaggt gatgttctga tcaacagcgg agatccatta aggatctccg
    24301 tgagactata gaatgcctga tgcgctacgc tcatcaggca tacaggactt ccgccactac
    24361 attaaggaaa agttatggta tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc
    24421 aactggcctt aggcggctgg caaatttctc gttttaaccg tgccttcgac acactatgcc
    24481 agcaagggcg ggttggcgtg ggtcgttcca gcgggcgctt taaaccgcgg gtcgtggtcg
    24541 ccatcggctg gacgatcagc agcgcatcgt cgacaccttg tttatgaaag gactgaccgt
    24601 cttcgcccga ccgcaaaaaa ttcccgcaat taccggtatg catgcgggtg atttacagcc
    24661 cgatgtgatc tttccccatg atccactatc acagaatgct ctatcattgg cgcttaaact
    24721 gaaacgtgga taatttcgtt gtgaatgtta cttgcttgcg aagttatcat tttgaaacct
    24781 aaatcaggta atcacgccca tgaaacctcg tcagcgtcag gccgccattc tggagtatct
    24841 gcaaaagcag ggtaaatgct cggttgaaga attggcgcaa tactttgaca ccacaggcac
    24901 aaccattcgc aaagatctgg tcattctgga acatgccgga accgtcattc gtacttatgg
    24961 cggagtggtg ttgaataaag aggaatccga tccgcctatc gatcataaaa cactcatcaa
    25021 cacccacaag aaagagctga ttgcagaagc tgccgttagt tttatccatg atggcgattc
    25081 gatcattctt gatgctggca gtaccgtttt gcagatggtt cccctgctct cgcgctttaa
    25141 taacatcacg gtgatgacca acagcctgca tatcgtcaat gcgctatccg aactggataa
    25201 cgaacaaact atcctgatgc caggcggaac gtttcgtaaa aaatcggcct catttcacgg
    25261 acagctggca gagaatgcct tcgaacattt cagtttcgat aaattgttta tgggcaccga
    25321 cggcatcgat ctcaatgcgg gcgtaaccac cttcaacgag gtttataccg tcagtaaggc
    25381 aatgtgcaat gccgcgcgcg aagtgatttt gatggcggac tcatcaaagt ttggccgtaa
    25441 aagccccaac gtagtttgca gtcttgaaag cgtcgataag ctgattaccg acgcaggtat
    25501 cgatccggcg tttcgtcagg cgctggaaga gaaagggatc gatgtgatca taaccggaga
    25561 gagcaatgag tgaagcacta ctgaacgcgg gacgtcagac gttaatgctg gagttgcagg
    25621 aagcaagccg tttaccggaa cgtctgggcg atgattttgt tcgcgccgcc aatatcatcc
    25681 tgcactgcga aggcaaagtg gtggtttcgg gaattggcaa atcgggccac attggtaaga
    25741 aaatcgccgc aacacttgcc agtaccggca caccggcttt ttttgtccat ccggcagaag
    25801 cactgcacgg cgatctgggg atgattgaaa gccgcgatgt gatgctgttt atctcttact
    25861 ccggtggcgc gaaggaactg gatctgatta ttccgcgtct ggaagataaa tctatcgcgc
    25921 tgctggcgat gaccggcaaa ccgacgtcac cgctgggcct ggcggcaaaa gcggtgctgg
    25981 atatctccgt agaacgcgaa gcctgcccga tgcaccttgc gccgacctcc agcaccgtca
    26041 ataccctgat gatgggtgac gcgctggcga tggcggtcat gcaggcgcgc ggatttaatg
    26101 aagaagattt tgcccgctcc cacccagccg gggcactggg cgctcgcttg ctgaataaag
    26161 tgcatcatct gatgcgccgt gacgatgcca tcccacaggt ggcgttaacc gccagcgtga
    26221 tggatgcgat gctggaactc agccgcaccg gtctggggct ggtggcggta tgtgacgctc
    26281 aacaacaggt acaaggcgtc tttaccgacg gcgatttacg tcgctggctg gtaggcggcg
    26341 gcgcactcac cacgccagtt aatgaagcga tgacgacagg cggcaccacg ttacaggcgc
    26401 aaagtcgcgc catcgacgcc aaagagatcc tgatgaagcg caaaatcact gccgcaccgg
    26461 tggtggatga aaacggcaaa ctcaccggcg caattaacct gcaggatttc tatcaggccg
    26521 ggattattta atccttcatt cccagacgtt tcgccagccg atgcaggttg gcgacgtcgg
    26581 tttccagcat ccgcgcgcag gcagcccagt tatgatgatt ttgtgccagt gcctggcgaa
    26641 tagtttcacg ctggaacgct tctgtcgctt cacgcaggtt ttgcttaaca acgggcaccg
    26701 ccgccgcttc tggcggcggc aacgtcacct caggaaaagc aaaatgttgc gcctcaagaa
    26761 tcacttcatc gccgttgcgg gtggctctcg ccagcactac cgcccgatga atagcatgtt
    26821 ccagttcgcg cacgttcccc ggaaaacgat agtgttgcag taaatttcgc gctccggcac
    26881 ttaataccac gcgggagagc cccagccgca aacgacactg ttcgcagaaa taccccgcca
    26941 gcagaatgac atcatcgccc cgctcacgca gcggcggcac cgaaagtgga aacacgctca
    27001 ggcgatgaaa caagtcagcg cgaaatcgcc ctgccagcac ctcttcgcgt aagtcgcggt
    27061 tagtcgccgc cagcacgcgc acatcgaccc gcaaactacg gtcatcgcca acgcgctgaa
    27121 tatcgccata ctgcaacacc ctcagcagct tggcctgcaa tgccaacgac aactcgccga
    27181 tctcatccag aaacagagtg ccgttatccg ccatttcaaa cttcccgctg cggttactga
    27241 tagcgccagt aaacgctcct ttcacatgcc cgaacaactc actttccgcc acactttccg
    27301 gcagtgcagc acagttgaga tagaccagcg gattcaccgc ccgtggcgag gcttcatgaa
    27361 tcgctttcgc caccagctcc ttaccggttc ccgtctcacc gctgatcagg acgttgagat
    27421 cggacgccgc cacaatctca atctcttttt tcagttgcgt catgccaggg gacaagccaa
    27481 tcatctgcgt ctgtttcacc gcttcaaacg gcgtggcatc gcctggcatc atattctggc
    27541 tttccagttg ttcaatcagc aacgcattgc ttaacgctcc cgccgccagc gcagcaatca
    27601 gccgtagctc ttcgtcgctg aaaacatcga actgatcggg ctgcatcccg tcgagcgtca
    27661 gtgcgccgat caggttttgc ccggcaaaca atggcagacc aacgcaggcg tgaaccttca
    27721 gactctcctg cccaggaatc aaaccgtcat agggatcggg caattcgctg tctgcgggaa
    27781 agcgcaccac atccccggcg cgggcaatcg cttccagccg tggatgccct tccagcgcaa
    27841 agcgtctacc gagtacaccc tttgccagac cgtcgatggc aagcggaata aactgccgcg
    27901 aatcgtaacg tagcaacgca gacgcatcgc actccagcac ctgacgtagc gtggtgatca
    27961 ggcgctgaaa acgatcctga tgaccaatcc cacgctgcaa ttcgatggcg atattcgcca
    28021 gcacatcaac ggaaaaactc atctttgcct cactgtcaat ttgactatag atattgtcat
    28081 atcgaccatt tgattgatag tcattttgac tactcattaa tgagcataat tttatttata
    28141 gagtaaaaac aatcagataa aaaactggca cgtaatctgc aattagcaag acatcttttt
    28201 agaacacgct gaataaattg aggttgctat gtctattgtg gtgaaaaata acattcattg
    28261 ggttggtcaa cgtgactggg aagtgcgtga ttttcacggt acggaatata aaacgctgcg
    28321 cggcagcagt tacaacagct acctcatccg cgaagaaaaa aacgtgctga tcgacaccgt
    28381 cgaccataaa ttcagccgcg aatttgtgca gaacctgcgt aatgaaatcg atctggcgga
    28441 tatcgattac atcgtgatta accatgcaga agaggaccac gctggggcgc tgaccgaact
    28501 gatggcgcaa attcccgata cgccgattta ctgtactgcc aacgctatcg actcgataaa
    28561 tggtcatcac catcatccgg agtggaattt taatgtggtg aaaactggtg acacgctgga
    28621 tatcggcaac ggcaaacagc tcatttttgt cgaaacgcca atgctgcact ggccggacag
    28681 catgatgact tacctgacag gcgacgcggt gctgttcagt aacgatgctt tcggtcagca
    28741 ctactgcgac gagcatctgt tcaacgatga agtggatcag acggagcttt tcgagcagtg
    28801 ccagcgttac tacgccaata tcctgacgcc gttcagccgc ctggtaacac cgaaaattac
    28861 cgagatcctg ggctttaact taccagtcga tatgatagcg acctctcacg gcgtggtatg
    28921 gcgcgataac ccgacgcaaa ttgtcgagct gtacctgaaa tgggcggctg attatcagga
    28981 agacagaatc accattttct acgacaccat gtcgaataac acccgcatga tggctgacgc
    29041 tatcgcccag gggattgcgg aaaccgaccc acgcgtggcg gtgaaaattt tcaacgtcgc
    29101 ccgaagcgat aaaaacgaaa tcctgaccaa tgtcttccgc tcaaaaggcg tgctggtcgg
    29161 cacttcgacg atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg aggagatgac
    29221 tggtttacgc ttccgtaaca aacgcgccag tgctttcggc tctcacggct ggagcggcgg
    29281 tgcggtggat cgtctttcca cgcgcctgca ggatgcgggt ttcgaaatgt cgcttagcct
    29341 gaaagcgaaa tggcgaccag accaggacgc tctggagtta tgccgtgaac acggtcgcga
    29401 aatcgcccgt cagtgggcgc tcgcgccgct gccgcagagc acggtgaata aggtagtcaa
    29461 agaagaaacc tctgccacca cgacggctga cctcggccca cggatgcagt gcagcgtctg
    29521 ccagtggatt tacgatccgg caaaaggcga gccaatgcag gacgttgcgc caggaacgcc
    29581 gtggagtgaa gtcccggata acttcctctg cccggaatgc tccctcggca aagacgtctt
    29641 tgaagaactg gcatcggagg caaaatgagt aacggcattg tgatcatcgg ttcgggcttc
    29701 gccgcccgcc aactggtgaa aaatattcgc aaacaggacg ccactattcc attaaccctg
    29761 attgccgccg acagcatgga tgagtacaac aaacctgacc tcagccatgt tatcagtcag
    29821 gggcaacgtg ccgatgacct tacccgccag acggcgggtg aatttgccga gcagtttaat
    29881 ctgcacctgt ttccacaaac ctgggtgacg gatatcgatg ccgaagcccg tgtggtgaaa
    29941 agccagaata atcagtggca atacgacaag ctagtactgg caaccggtgc cagtgccttt
    30001 gtcccgcctg agcctgggcg tgagttaatg ctgacgttaa atagtcagca agagtatcgc
    30061 gcctgtgaaa cgcaactgcg ggatgcccga cgcgtgttga ttgttggcgg tggtttgatt
    30121 ggtagcgaac tggcgatgga tttttgtcgt gcaggcaaag cggtcacgct aatcgacaac
    30181 gctgccagta ttctggcgtc gttaatgcca ccggaagtaa gcagccgctt gcagcatcgg
    30241 ttgacggaga tgggcgttca tctgctgttg aaatctcagt tacaggggct ggaaaaaacg
    30301 gattctggca ttctggcaac gctggaccac cagcgtagta tcgaagtgga tgcggtaatt
    30361 gccgccaccg gactgcgccc ggaaacagcc ctggcacgac gcgccgggct gacgattaat
    30421 cgcggcgttt gcgtcgatag ttatctgcaa accagtaatg acgatattta tgcgctgggc
    30481 gattacgcgg aaatcaacgg tcaggtactg ccattcctgc agccgattca acttagcgcg
    30541 atggtgctgg caaaaaatct tctcggcaat aacacgccgc tgaaactacc gacgatgctg
    30601 gtgaaaatca aaacgccgga attaccgctg catctggcag gcgaaaccca gcgtcaggat
    30661 ttacgctggc aaattaatac cgaacgctag ggaatggtgg ctcgcggtgt tgacgatgct
    30721 gaccagcttc gcgcctttgt ggtcagtgag gatcggatga aagaggcatt tggattgttg
    30781 aaaacattgc cgatgtaggt gggctactgt gcctaaaatg tcggatgcga cgctagcgcg
    30841 tcttatccga cctacgggga cgcatgtgta ggccggataa ggcgtttacg ccgcatccgg
    30901 caatggtgtc caaatgcaac acgttttatc cgttctggac ttcacccgct aaccaacgcg
    30961 ccgcagcaat aaccccctgc cccagagaca aaccgccatc acccgccggt aaactctgtg
    31021 gaaagagcaa tgtgaaatca gcgagataat gcgccagacg tgcacgcagc aaacggttat
    31081 gaataacccc gccgctaaat accagcgtag tgataccacg catcgtggcc tgctcacgca
    31141 tcaacgcggc aaaaccctgc gccagcgcat catgaaacgc ccacgcgcgt tgattaacct
    31201 gtgcctgcca gttcagccac tgctgccaga aagcggcgag atccagttga ttgtccacca
    31261 gcggcatcgt caccggatgc gtcactccgt ggcatgaggc tgcgagcgcc tccagagcac
    31321 aagccgcttc accttcataa cttaacgtgg ctggcgcaca gcccagtgcc gccgccactg
    31381 catcgaacaa ccgcccacac gatgacgcca gcggcgcgtt aattccacgc tcaatggccc
    31441 gcgccagcac gctccagttt tgctgttgca cacttgctgt ttcagggtaa ttctgccact
    31501 ccggcacaaa gcgcaggcac tgcgccagca ggtttcgcca cggctgcttc gctgccaaat
    31561 cgccacccgg aagcgccact gcaggcaagc cgcccaggtg ctcacattcg cgatagttca
    31621 cccgcaggca ctcgccgccc cacaaagcgc cgttctcccc cataccgata ccgtcgagcg
    31681 tcaaagcaat gacatcaccg ccatccagcg gccactgatg ctctgccaga cacgccgctg
    31741 catgggcatg atgatgcagt accgtttgcg tcggcagatt catttcacgc gcccactggc
    31801 tggagacata gcccggatgc gcgtcatgca caacgtattt cggggtaaaa tcgtagatgt
    31861 tttgcatcag gcgtaacgct tcgcgccact gcatctggat gccatcgtca cttaaatcgc
    31921 ccagatgctg actcaacacc gcttgttcac cgcgcaccag gcagaaggta tttttcagat
    31981 ccgctccgag acacagcaca ggcggaacat ttttaaagcc cggaggcaaa gccagcgcat
    32041 ccggcacata cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc accgaatcat
    32101 ccatccgctg cacgatgtcg cggttatgta tcaagaatcc gtcggcaatg ccctgcaaat
    32161 ccgccagcgc ctgttcgttg ctgatagctg gtggtttacc gctcaggttg ccggaggtca
    32221 tcaccagcgg gcattgcagt tcctgtaaca gcaaatgctg gagcgggttc gcaggcaaca
    32281 ttaccccgac ttcgttaagg tcaggggcga tatcatcaca aagctcagga acgtattttt
    32341 tatccaccag cacaatcggc gcggcgggcg tggtaagcaa ctggcgcgca gcgtctggta
    32401 aaccgtcagc cactggcaac atgaccgcca gcggtttcgc cgggcgatgt ttgcgcgccc
    32461 gaagtgtcgc caccgcgtta ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc
    32521 ctttgatggc gacaatgttg cccattttta actgtgcgat agccgcctgt aatgccgcct
    32581 cttgttctgc atgttcacca tgacttaccc attcaagatg cgggccacac tccgggcagg
    32641 ccaccggctg ggcgtggaag cgacgatcga gcgggtcgcg gtactctttg tcacaagccg
    32701 gacacaacgg aaacgccgcc atcacggtaa acgggcggtc gtaaggcatg gcgcgaataa
    32761 tggtgaaacg cgggccgcag tgggtacagt tgataaacgg ataacgataa cgccgttcgc
    32821 ctggggtatt catttcggca aggcaagcag ggcaagtagc ggcatccggg acaatttgcg
    32881 tattcatggc acctcccgcg ctctggcgga tggtgaactc agtgggcagt tgtgaccaga
    32941 tgtacggctc acgctcgacg ctatcaatac gcgccagcgg cgggcagtgc tgatgcaatt
    33001 gaacaagaaa cgtttccggg tcttcccgca gccggacttc tacgccatcg ccgtcattac
    33061 agacatcgcc gtgaagattt aattgctgtg ccagctgcca gacaaacgga cgaaaaccga
    33121 cgccctgcac tttgccacga atacgcagtt ggacaccgca agatgtgttt tttgccattg
    33181 agttattccc gccatcatga attgcgtaac ccgcccagcc ggacacgaca gcgtcgcatc
    33241 cggcagtcac aggtcggcga taccgcccgc tccgtattct acgaatattt ccgggaattc
    33301 ctttgatgcc agaacagttc tgtaagattt ttagaacatc agcgccgtac ggcggcgttt
    33361 ttctgcactc agttgttcaa gtttattacg atcgacacaa atcagcgcat gagtcgggca
    33421 agccgccata cacgccgggc cgtcttcacg atggttgcac aggtcacatt tattggcttc
    33481 ggctttgtca gcccgtacat tcagccccgc gccgctgttg cgaatcaccg gacgtaccac
    33541 cacttccatc gcgccatacg ggcaagccac aacgcaggtt ttgcaaccaa tgcaacgttc
    33601 ctgcatcaca tgaacaaacc ctttatcacg gctgatagca ccattcgggc agacgttagc
    33661 gcacggtgca tcttcacact gacggcaaac tgtcgccgtg gaaatgttca cacctttaat
    33721 gacatggata cgcggtaaaa atgtttccgg ggtcagcgat gcacagtcct gattttcctg
    33781 atgagaaacc acgcacgcca cttcacaggt acggcaacca atacatttac tcgcgtcagc
    33841 aatgatgaaa cggttcatca aattctccag caatgacagt taatgcgccg atacattcac
    33901 aaatcatgcc agtttttaat tcactgttat ttaaggaaat taatttctgt aatgcaggaa
    33961 aaaagatgtc atcgacacta gtgacgatga catgtgatga caatgtttat cgcgaaagag
    34021 caatgagtga gtcgcggtgg atcagttttc cgctgaaggt tttcggcggt gagaaatccc
    34081 cgccatcgag cataaaaatc agccgtccaa taatttcctg aatcatctca gtcaccggaa
    34141 tttttacgct ggagagcgcc ggaacgatat agggggcaat tgcgatatca tcgaatccga
    34201 taactgacac ctgctctggc accgctacgc cgcgctcgtg caacgctttc atcgcaccta
    34261 tcgccatatc gtcgttactg gcaactaacg cgctaaattt agccccacgt tcgagcaacg
    34321 tttctacccc ttcggccccg ctggcgggcg tccatttacc gttagcgata agtttttcat
    34381 tgagcgcaat accatgctgc gccagcgcgt ctttataccc agcaagacgt tcaatgctgg
    34441 tgggggaatc catcgagccg gtaaggaaag caatctcctg atgtccggcg tttatcaact
    34501 ctgccacggc attaaagctg gtctgtttat gatcgcacca gacgctatgg ctgctgtttt
    34561 tgcgcaggcg gcgattaagc accattatcg gctgactgtg cgcgtcaatg atgtcatcga
    34621 tctcatccac gcttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc aaatccagca
    34681 gatactgaat cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc aatagcaact
    34741 gccgcccttt ctcttccgcc attcgcgcgg catgaaagag taattcacta aaataaatgc
    34801 cgtggtaaag cgtgttggtc actaccagcc ccagcgtctg agtactcttc gccgacagat
    34861 tgcgcgccag caagtttggg cggtaaccgc tctcttctac cgcctgaaac acgcgatctt
    34921 tagtctcctg gctgacgtag ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa
    34981 ccccggcgcg cttcgccact tccagcatcg tcgtcatcat tttcatccct ttacacgcaa
    35041 tcaacgcagt gtactgcacc gtttgccgat tgtccttgcg caatcggcgg gaaaaatatt
    35101 caggtgaccg gtttcacaaa tataaaaaat gaataattaa ctctcttgct tatttagtga
    35161 caactattca tgattttgtg aaaccggttt cctaattccg tttcagcatc ggcatttttc
    35221 cgttacgtcg actgataaca actacatcta ccctactgat aacaggataa aatccgatgg
    35281 ccaaaaatta tgcggcgctg gcacgctcgg agatagcggc actgggcggc gttgataaca
    35341 tctcggcggt cacgcactgt atgacgcgtt tgcgctttgt tatcaaagat gaccagctta
    35401 tcgacagccc gacgttaaaa accatccccg gcgtgctcgg cgtggtacgt agtgacaacc
    35461 agtgtcaggt gattatcggc aataccgttt cacaagcctt tcaggaagtc gtcagcctgc
    35521 tgccgggaga tatgcagccc gcacagcccg tgggtaaacc caaactcacg ccgcgtcgca
    35581 ttggtgcggg gatcctcgat gcgctgatcg gcaccatgtc accgctgatc ccggcgatta
    35641 tcggcggatc gatggtcaaa ctgctggcga tgatcctcga gatgagcggc gtgctgacaa
    35701 aaggatcgcc gaccttaacc attctgaatg tgattggtga cggtgctttc ttcttcctgc
    35761 cgctgatggt cgcggcatct gccgccatca aatttaaaac caatatgtcg ctggcgattg
    35821 ccatcgcggg tgtgctggta catccgagtt ttattgaact gatggcgaaa gcggcccagg
    35881 gtgaacatgt cgaatttgcc ctgattccgg tcaccgcggt gaaatacacc tacacagtga
    35941 tcccggcgct ggtgatgacc tggtgcctgt catatatcga acgctgggtg gatcgcatta
    36001 caccggcggt gaccaaaaac ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc
    36061 tggcaatcct gctgattggc ccgattggta tctggatcgg tagcgccatt tcggcgctgg
    36121 tttacagcat tcatggttat ctgggctggc tttcagtcgc cattatgggc gcgctgtggc
    36181 ctctgctggt aatgaccggg atgcaccgcg tctttacgcc aaccatcatt cagaccattg
    36241 ccgaaaccgg caaagaaggg atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg
    36301 gcggttcatc actggcggtg gcgtggaaaa cgaaaaaccc ggaactgcgc cagacggcgc
    36361 tggctgcggc ggcatcagcc attatggcgg ggatttccga accggcgtta tacggcgtgg
    36421 cgatccgcct gaaacgtccg cttatcgcca gtcttatcag cggttttatt tgcggcgcgg
    36481 ttgccggtat ggcggggctt gccagccact caatggcagc gccggggcta tttaccagcg
    36541 tgcaattctt cgatccggcg aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc
    36601 tggcggtggt gctgtcgttt atcctcacac tgttgctcgg ctttgaggat attcctgttg
    36661 aggaagcggc tgcccaggcg cgaaagcatc agagcgtaca accgaccgtc gccaaagaag
    36721 taagtcttaa ttgaggatga aaatgtcagt atttccagaa ggttttttat ggggcggcgc
    36781 gcttgccgcc aaccagtctg aaggtgcgtt ccgtgaaggc ggaaaagggc tgaccaccgt
    36841 cgatatgatc ccacacggcg agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt
    36901 tcagttgcgc gatgacgagt tttatcccag ccatgaggcg acggattttt atcatcgtta
    36961 taaagaagat atcgccctga tggcagagat gggattcaag gttttccgta cctcaattgc
    37021 ctggagccgt ctctttccgc agggcgatga aatcacgccc aatcagcagg gcattgcttt
    37081 ttatcgttct gtctttgaag agtgtaaaaa gtacggtatc gaaccgctgg tcacgttgtg
    37141 ccacttcgat gtgccgatgc atctggtcac cgaatatggc tcctggcgta accgcaagct
    37201 ggtggagttt ttcagccgct acgccagaac ctgctttgaa gcatttgatg gtctggtgaa
    37261 atactggcta accttcaatg aaatcaacat tatgttgcat agcccgttct ccggcgcggg
    37321 tctggtgttt gaagaaggtg aaaatcagga tcaggtgaaa tatcaggccg cgcatcacca
    37381 gctggttgcc agtgcgctag ccgccaaaat cgcccatgag gttaacccgc aaaatcaggt
    37441 gggctgtatg ctggcgggcg gcaacttcta cccttacagc tgcaaaccgg aagatgtctg
    37501 ggcggcgctg gagaaagatc gggaaaacct gttttttatc gatgtgcagg cgcgcggcgc
    37561 gtatccggct tactctgccc gcgtatttcg cgaaaaaggg gtaaccatag ataaagcgct
    37621 gggcgatgat gagatcctga aaaacaccgt cgattttgtc tctttcagct attacgcctc
    37681 gcgctgcgcc tcggcggaga tgaacgccaa caacagcagt gcggcgaacg tggtgaaatc
    37741 gctgcgtaac ccgtatctgc aagtgagcga ctggggctgg ggaattgatc cactcggtct
    37801 gcgtatcacc atgaatatga tgtacgatcg ttatcagaag ccgctgtttc tggtggaaaa
    37861 cggcctcggc gcaaaagatg aacttgctgc caatggcgag attaacgatg actatcgcat
    37921 cagctattta cgcgaacata tccgcgcaat gggcgaagcg attgcagatg gcattccgct
    37981 gatgggctac accacatggg gctgtattga tttagtttcc gcctctacgg gtgaaatgag
    38041 caaacgctac ggttttgtct acgtagaccg tgacgacgca ggcaacggca cgctgacgcg
    38101 cacacgtaag aaatcgttct ggtggtataa aaaagtgatt gccagtaatg gggaagattt
    38161 agagtaagta acagtgccgg atgcggcgtg aacgccttat ccggcctaca tgtcgcacga
    38221 accaggaagg cccgataagc gcagcgcatc gggcaattta gcatttggca tcagagctgg
    38281 ttcaccccat caagaacatc cctgtcctga ttccttaatg aaaaactact cttcttccac
    38341 cgctaactgc gcgaagccgc cattcccttc ccagccttcc agtcgttgat aaacggtttc
    38401 taccgcatct ttaatcggct gggtcatcgg gtagtaaaag ccgacgatat ccggctgtat
    38461 gccgaggaaa atcacttcgc caatatcttc tttcaactgg tcgataagat agttcagagg
    38521 catgttatgg gtagtcatca taaacatctc ggcgatatca tccgggtcga tgatgcggat
    38581 ctcgccgggg tttagcccca tatccgtggc gtcgacaatc agtaaccgcg tagggcgcag
    38641 ttcacggatg gcgacgatgt cgttttccgg tgcactaccg ccgtcaatca ccacccagtt
    38701 acctttcggc gcggcggcgc acttttccgc cagcagcgga cctgcgccat catcgcccat
    38761 catgctattg ccaacacaga gtaaaacgtc agtcacgcaa tctcctcacc atcaaataga
    38821 tggcgttttc ctgatgaata tcatgcagca tacttaacag cattgtactc cattcctgct
    38881 gctgcgaagt ttgcgctgcc cgggctttat cgaatgcgtt ggccagcatc ggcacatggt
    38941 taatgtcgat gacgatctca ccgtacttcg gtacgccttc cattttgcgg cgggcttcac
    39001 tgcccgcctc cagagtggca atccatgcca gatattcatc ccacgggcag gtgagcgccg
    39061 cttccaggca atcgataacc ccaaggtggt gaccaatcgc caggctgtaa tagaccacct
    39121 gctgcgcctc ggcgggcgtg gcatcgttct catcaataaa tttacggctc agttgactga
    39181 acaccacctt ttcactcatc ggatacgcac ctcttctaca acatgattca gatggctgac
    39241 aatctcgttc agacgcggat cgttttccgc ttccagccag cgtgcaacct gttcttcgcc
    39301 ctgccctaac tgtgtcagga agtcatcggc aatctgacga ccgtaacgat aacccgccag
    39361 gcggcgtgct tcgcgatcca ctttcacgcg cagcggctgc accatatcgc catgcaggat
    39421 ctccgccggt tgttcatcca gttcacccgg cccacgggcg tgaattttct gctccagcag
    39481 gccgagcgcc attgcaaagc cgtacagcgt ggcggcaggc gttggcgggc agccagggat
    39541 ataaacatcc acagggacaa ttttatccgt accgccccac acgcagtaga gatcgtggaa
    39601 gatcccgccg ctgttaccgc aggcaccgta ggagatacaa attttcgggt ccggcgcgga
    39661 ctgccacgca cgcagcgcag gggatcgcat tgcacgggtg accgcgccgg taaacagtaa
    39721 aatatccgca tgacgcggtg aaggaacgac tttaatgccg aagcgttctg catcaaacag
    39781 cggcgataaa gtggcgaaaa tttcgatttc gcagccgttg cagccgccgc agtccacgcg
    39841 gtaaacatag gcagaacgtt tgattttttt cagtaacgac gccttcatgc tggcgatgga
    39901 ttcatccacc gtcatcggga ccggaatgcc gttggcgtca cgggggccta ataaattgct
    39961 catcagatgg cctctttcat atggcgagtc agttcaatac ggtcggacgg caccaggcat
    40021 ttctggcgct tacattccgg gcaagtctca aagctttcgc ggtggttttc cgcgcggctg
    40081 tcgccgttgt gcttaagcag cgcaatggcg tagtcgatct ctttctggac ggcgaaagga
    40141 cgattgcaga cgcgacagtt gcacagcgcg aagcgagact gttgcaggaa gtcttctttc
    40201 ttccacaccg ccagttcgta ctcttgcgac agcttaatcg ccgccgtcgg gcaaacttct
    40261 tcgcagcgtc cacagaagat gcagcgcccg agattaaact cccaggccag ctctcctgtg
    40321 gcgaggtcag tttcaaccgt taaggcgttt gacgggcagg cattgacgca ggccgcgcag
    40381 ccgatgcact gctgcgggtt ctgctctggc ttaccacgga agtttttatc aaccgcaatc
    40441 ggctccagcg gataagacga ggtcgccgta ccggttttga taactttttt gataaaggta
    40501 aacatggcga ttccttattt cagcggcgag tttttacgct caatgctgta gcgctcgagt
    40561 tctttgtacg gcaccacttt gctcttcttc ttacgtacat cgaccacagt catgcggtcg
    40621 gtacaggagt agcaagggtc caggctaccg ataatcagcg gcgcatcgga aacagtgttg
    40681 ccacgcagca tgtagcgcag ggtcggccag ttcgcgtagg tcgcggcacg gcagcgccag
    40741 cggtacagct tctggttgtc gccagtcatg ctccagtgga tatcatcacc gcgcggcgct
    40801 tcggcaaagc ccagcgcgaa acggtgcgga atgtaggtaa agccttccac catcagcggg
    40861 ccacccggca ggttatccag accgtagtcg atcatgttca gcgcggtata gacttcgttg
    40921 atacgcactt tcagacgcga aataacgtcg cagccctgct cgctgtggac ttccattggc
    40981 agcaggccat aaccaacaaa cgggtgatca gcgcgggtat cgcgagcgtg tccgctggcg
    41041 cggaccatcg ggccaacgtt actgaaatcg cgggcaattt ccgggtccag acgaccaatg
    41101 ccgacggtgc gctgttccat gttcggtgtg ctcagcagca catccaccag ctcctgcact
    41161 tcacgacgca tctgttgcgc cagctggcgg gtctggatca tgtcatcttt caacagatcg
    41221 cgacgaatcc cgccgatcag gttcaggccg taggttttac gcgcaccggt gaggatctcg
    41281 gccattttca tggaggtttc acgcacgcgg aagaattgca taaagccgga gtcgaagccg
    41341 gtaaagtggc aggcgaggcc gaggttgagc agatgcgagt gcaggcgttc cacctccagc
    41401 agaatggcgc ggatcatctg cgcacgttct ggcacctgaa tacccatcgc gttttccacc
    41461 gacgtggtgt aggcggtgct gtgggcgaag ccgcagatcc cgcacacacg gtcagagagg
    41521 aaggtcactt cgttataacc catgcgggtt tccgccagtt tttccatacc gcgatggaca
    41581 tagaacaagc ggtagtctgc gtcgataatg ttttcgccat cgacgaacag acggaagtgg
    41641 cccggttcgt cagaagtgac gtgcagcgga ccaatcggca cgacgttgtt tttcttgtcg
    41701 cccagttcgt tgatgaactc gtaggtttca gcatcggtgg tcggtgccgg acgctgacga
    41761 taatccatgc tgtctttacg cagcggataa agttcatccg gccagtcatc cggcagcacc
    41821 agacggcgtt catccggcag accaaccgga atcaaaccgt acatatcgcg cacttcgcgc
    41881 tcgccccaca ccgccgccgg aacgcgcggc gtcacggacg gatactccgg tttgttggca
    41941 tcaacttcga cgcgaaccgt tacccagcac ttagtcccct tctccatcga cagcacgtaa
    42001 taaacggcgt aatgaccatt cagtttgcgt tcgtcgttac caaaaagcac cgacagccag
    42061 cccccctgct tgtagtagag aaactccacc acttccggca gatagttcac ctttacggtg
    42121 acagtcagct gctctttggt ctgccaggcg tggtccagca cgacgcccgg aaatgcctca
    42181 ttcagcgcgg cgagataatg ttgacctaat ttttcttcag acatgctcaa actctcttta
    42241 atcacgccgc cagcaaggag acgaacgcta aaaatgcaaa gccaaaccca gcccaggtaa
    42301 tgcgcgaagt aatatcaaga cgcagacggg ccatgctgtt ttcgaacagc gcgataacca
    42361 ggacgccgac caccagtttt acgatcgcaa tcaccagcgc cagcagcagt ccaccggcgg
    42421 tgaaggtttc catttgtccc cacggaataa acaccccgac gaacatctgc aacaccacca
    42481 gctgtttcag gctgataccc catttcatga cgccaaagcc gctgccgcta tactcagaga
    42541 gcggaccttc ctgcaactcc tgctcggctt ccgccaggtc gaacggcagt ttgcccattt
    42601 cgataaaagt ggcgaacgca caggcgcaga gcgccagcac cagcgggatg ctttggctca
    42661 gcggccagtg ataaacggtg tcggtaatgt tgctgatgtt ggtggaaccg gcaacctgtg
    42721 cggcaaccca cagaccaagc agcaacatcg gttcgaccag cacgccaagc atcgcttcac
    42781 ggctcgcacc gatagcggta aacgggctac cggtatccag accggaaatg gcaaagaaga
    42841 aacgcgcgat ggcgaagaga tacagtaagg tgatcaaatc gcccagttgc ggcagcggag
    42901 aaccgacggt caccaccggc agcgcagtag cgatagtcag catgacgccc accatcacat
    42961 acggcgtcag gcggaacacc cagccagagg catccgggcc aacgctctgg cggcccagca
    43021 gtttgataat gtcgcgatac tcctgcaaca cgcccggccc gcgacggtta tgcagacggg
    43081 cgcgcgccac gcgggtaata ccggagagca gcggcgcaac ggcaaataac accagcgcct
    43141 gaattaacgg atataaaaca ctcattctca ggctcctcgt gaaacaataa tcaccaccag
    43201 taccgccagt tcaaccagcg ccatccggcg gaacagcaac gcactacctt cgcactgcca
    43261 gcccggcacc agcgacaccg gattcagcca tttgcgcagt ttcagcactg gcgcaaacgc
    43321 ctgtttaacc ggcatggcaa aaccgtgagc ggtaatcacc attgattttt catggtcgta
    43381 gccgcacacc caggccgcac cgcgggaacg tgacggcagg cggtcgcctt tgcaaatcgc
    43441 cataatgatg aatggcagca gcgggcaggc aatcagcagc aatgtgatca ttggttgaga
    43501 aacggtggtg ttagcaggct ccagaggcag aggtacggca gcagagagca tcggcagcag
    43561 ccacggcgca gcaacaccgc caattacgca gcaaatcgcc agtgccacta cgcttacgct
    43621 catcaggagc ggcgcacagg tggcgttttc ggcttctttg gtacgcggcg cgccgaggaa
    43681 cgtgacgcca tagactttcg ccatacacat caccgccagc gcaccggtaa ttgccagacc
    43741 cacagcgagc agcggcccca gcagacgggc aacaaacgcg ccactattgc tcagtttgaa
    43801 aaaggattga tagataaccc attccccggc aaacccgttc agcggcggca gcgcagccat
    43861 tgccatcaac ccgactaaca tggcgatgga gataaccggc attttcttgc caataccacc
    43921 gagtttttcg atatcgcgat gaccggtacg gaaccagacg ctccccgccc cgaggaacag
    43981 tacgcttttg aacaggctat ggttaagcag atggtacaga ccaccgacca ggccaagagc
    44041 aatcagcgcc ggttgttcga gcgcgatacc cgttacgcca gcgcccagcc ccagcaggat
    44101 aatgccgata ttttccaggg tgtggtaagc cagcaggcgc tggatgttgt gctccatcag
    44161 cgcatacaga ccaccgacaa acgcggtgat catgccgagc accagcagca cgatccccca
    44221 ccacagcggt gcattaccgc ccagcagtga caggattaaa atgcccagca ggccaatttt
    44281 catgactacc gtagaaaaca acgcggcagc tggcgcagag gcgttagcat gtgcctgcgg
    44341 cacccagccg tgcagcggaa taatcccggc gagcaggcca aagccaatca caccgagcag
    44401 ccagatatcg gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag
    44461 cgtgccataa cgctgccaca gcagccagca agcaatcgcc agcagcagag tgccaagacg
    44521 ccccagcgca aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacaa
    44581 ggccatgact tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcgcagac
    44641 ggcagcagcc atcaacatat tgatctgcaa gccgttgcac ttcacctgcg cgtggcgatg
    44701 ccagtcaatg ttgtagaggc tgacaaacag accgcacaga ccgagcgtaa tcagccaaat
    44761 cgcgttaagc ggggagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc
    44821 aaccgcgcca gtcagcacag tgaagcccgc ggctgccgta tacaggctac caaccgcgcc
    44881 gccaattccg gctatccagc cacttagcgt cttttgaaaa gaaaagagaa atgccagaac
    44941 agcggcggcg acaaaccaca ccacgccgct attgatcagg gaaattgcgc tcatttagcc
    45001 tctccacttt gagcctgctg aaacaaggtg agatcgccaa agtccgtatt aaaagtcagc
    45061 tcacgcttac gtttgctgac gcgggcgata tcggtgttat ccaccagatg cagggctttg
    45121 gtcgggcaca tccgcacgca ggccggacct tgttcatcaa agctacaaag gtcacatttg
    45181 acggcgatcg cgcgaatacc cggcacccag tcaagcagcg tactgatacg cgccggagca
    45241 ggcggtgccg gtggcgcttt cggggtattg gcatttgcag gaatatccag cggacggctg
    45301 ccggaaaatt caattgcgcc aaacgggcag gcgatgccgc acagcttgca gcttacgcac
    45361 aggctttcat tcaactgcac ggccccatca acgcgggtga tgacgttaac cgggcagacc
    45421 accgcgcagg gtgcatcttc acagtggtga cagagctgcg gcgcagattc tttttcattc
    45481 agcatcactc tcaggcgcgg cattgattgc aggccgtgct ggcgatgcgt ctctgaacag
    45541 gctgcctcac aagtgtggca gccgatacag agcgtggagt cagcaattac aaaacgattc
    45601 accaggcatt cctcaggtga ttgtcatttt tgacgaaaac atgccgatga aatgtcattt
    45661 tcgacactca tcgacacgcc catccccaaa caggcgtaac gcctgcaaaa cgggcaaagc
    45721 ctcagctcat gctgccgggc tttgtccctt taccagttgg cttaaattca ccggcacatt
    45781 gttttcaatg gcggtgtata agctgtcgta aacgccagcg attttttcga gatagtgttc
    45841 cagaacctgt tcgcgatcgc ggatgttgac cttgccatca atcatgccgt caatgctcag
    45901 ttgcgcctgc gcaatccgct gtttgtcttc gccctctttt gtttcgacgt aatactcgat
    45961 ggtgtggctg ttaaagccat ctgccagggt gacgtaaata cgaaaatgtt caaaaaggac
    46021 gaaacagagt tctttgtccg gcgcgcagct catggcgtga tgcaggcgcg ctttcgataa
    46081 cgtgatcccc tgaaccagcg aattgcagta gatgtgccac tggtcctgta ggcgacgatg
    46141 ccgctgtgcg atgtaatcgg ctttctcgct tatttcccaa atagtcattg tcaggttacc
    46201 cgtttaacag agatgccagc tttaagcatt ttctgtgcca atttttaatt tattgttatt
    46261 aaaacgattt ttaagctaaa gatgaatttc gtcgccgtgt cgacgtgtca tttcgacatc
    46321 atcgacatta ttcaccgcag ggataatcaa cactggcaca attattgctt gtagctggca
    46381 atagttaatg ggaggcgata tgcacgaaat aaccctctgc caacgggcac tggaattgat
    46441 cgaacagcag gccgcaaaac acggcgcaaa acgcgtaact ggggtctggc tcaaaattgg
    46501 cgcattttct tgtgtcgaaa ccagctctct tgccttttgt tttgatctgg tttgccgcgg
    46561 cagcgtggcg gaaggttgta aactgcacct cgaagaacaa gaggtcgaat gctggtgtga
    46621 aacatgccaa cagtatgtga cgctattgac ccagcgcgtc cgccgctgtc cacagtgtca
    46681 tggtgacatg ctacagattg tggcagacga cggtttacag attcggcgga tagaaataga
    46741 ccaggagtga gcgatgtgta caacatgcgg ttgcggtgaa ggcaacctgt atatcgaggg
    46801 tgatgaacat aaccctcatt ccgcgtttcg tagcgcgcca tttgccccgg cggcacgccc
    46861 gaagatgaaa atcaccggca ttaaagcgcc tgaatttacc cccagccaga ctgaagaagg
    46921 cgacctgcat tacggtcatg gcgaagcggg cactcacgca ccgggcatga gccagcgtcg
    46981 gatgctggaa gtcgaaattg acgtgctgga caaaaacaac cgtctggctg aacgcaaccg
    47041 cgcgcgcttt gctgcccgca agcaactggt gctcaacctg gtttccagcc caggttccgg
    47101 taaaaccacc ctgctgacgg aaaccctaat gcgcctgaaa gacagcgttc cgtgcgcggt
    47161 tattgaaggt gaccagcaaa ccgtgaacga tgccgcacgc attcgcgcta ccggcacacc
    47221 agcgattcag gtgaacacag gtaaaggctg ccatcttgac gcacagatga ttgccgacgc
    47281 cgcaccgcgt ctgccactgg acgataacgg tattctgttt atcgaaaacg ttggcaacct
    47341 cgtatgcccg gccagcttcg atctcggtga aaaacacaaa gtggcggtgc tttccgttac
    47401 cgaaggtgaa gacaaaccgc tgaaatatcc gcatatgttt gccgccgcct cgctgatgct
    47461 gctcaacaaa gttgacctgt tgccgtatct caactttgac gttgagaagt gcatcgcctg
    47521 cgcccgcgaa gtcaatccag aaattgaaat catccttatt tccgccacca gcggtgaagg
    47581 gatggaccag tggctgaact ggctggagac acagcgatgt gcataggcgt tcccggccag
    47641 atccgcacca ttgacggcaa ccaggcaaaa gtcgacgtct gcggcattca gcgcgatgtc
    47701 gatttaacgt tagtcggcag ctgcgatgaa aacggtcagc cgcgcgtggg ccagtgggta
    47761 ctggtacacg ttggctttgc catgagcgta attactgaag ccgaagcacg cgacactctc
    47821 gacgccttac aaaacatgtt tgacgttgag ccggatgtcg gcgcgctgtt gtatggcgag
    47881 gaaaaataat gcgttttgtt gatgaatatc gcgcgccgga acaggtgatg cagttaattg
    47941 agcatctgcg cgaacgtgct tcacatctct cttacaccgc cgaacgccct ctgcggatta
    48001 tggaagtgtg cggtggtcat acccacgcta tttttaaatt cggcctcgac cagttactgc
    48061 ctgaaaacgt tgagtttatc cacggtccgg gttgcccggt gtgcgtactg ccgatgggta
    48121 gaatcgacac ctgcgtggag attgccagcc atccggaagt catcttctgt acctttggcg
    48181 atgccatgcg cgtaccaggg aaacagggat cgctgttgca ggcaaaagca cgcggtgccg
    48241 atgtgcgcat cgtttactcg ccgatggatg cgttgaaact ggcgcaggag aatccaaccc
    48301 gcaaagtggt gttcttcggc ttaggttttg aaaccaccat gccgaccacc gccatcactc
    48361 tgcaacaggc gaaagcgcgt gatgtgcaga atttttactt cttctgccag cacattacgc
    48421 ttatcccgac gctgcgcagt ttgctggaac agccggataa cggtatcgac gcgttcctcg
    48481 cgccgggcca cgttagtatg gttatcggca ctgatgccta taattttatc gccagcgatt
    48541 ttcatcgtcc gctggtggtc gctggtttcg aaccccttga tctactgcaa ggcgtggtga
    48601 tgctggtgga gcagaaaata gcggcccaca gcaaggtaga gaatcagtat cgtcgggtgg
    48661 taccggatgc cggtaacctg ctggcgcaac aggcgattgc cgatgtgttc tgtgtcaacg
    48721 gcgacagcga atggcgcggc ttaggcgtga ttgaatcttc tggcgtgcac ctgacgccgg
    48781 attatcaacg attcgatgcc gaagcacatt tccgcccggc accgcagcag gtctgcgatg
    48841 acccgcgcgc gcgttgtggt gaggtattaa cgggcaaatg taagccgcat caatgcccgc
    48901 tgtttggtaa cacctgtaat cctcaaaccg catttggtgc actgatggtc tcctccgaag
    48961 gagcgtgcgc cgcgtggtat cagtatcgtc agcaggagag tgaagcgtga ataatatcca
    49021 actcgcccac ggcagcggtg gccaggcgat gcagcaatta atcaacagcc tgtttatgga
    49081 agcctttgcc aacccgtggc tggcagagca ggaagaccag gcacgtcttg atctggcgca
    49141 gctggtagca gaaggcgatc gtctggcgtt ctccaccgac agttacgtca ttgacccgct
    49201 gttctttcct ggcggtaata tcggcaagct ggcgatttgc ggcaccgcga atgacgttgc
    49261 ggtcagtggc gctattccgt gctatctctc ctgtggcttt atcctcgaag aaggattgcc
    49321 gatggagaca ctgaaagccg tagtgaccag catggcagaa accgcccgca cggcaggcat
    49381 tgccatcgtt actggcgata ctaaagtggt gcagcgcggc gcggcagata aactgtttat
    49441 caacaccgct ggcatgggcg caattccggc gaatattcac tggggcgcac agacgctaac
    49501 cgcaggcgat gtattgctgg ttagcggtac actcggcgac cacggggcga ctatccttaa
    49561 cctgcgtgaa caactggggc tggatggtga actggtcagc gactgcgcgg tgctgacgcc
    49621 gcttattcag acgctgcgtg acattcccgg cgtgaaagcg ctgcgtgatg ccacccgtgg
    49681 tggtgtaaac gcggtggttc atgagttcgc ggcagcctgc ggttgtggta ttgaaatttc
    49741 tgaatcagca ctgccggtta aacctgctgt gcgcggcgtt tgcgaactgc tgggactgga
    49801 cgcccttaac tttgccaacg aaggcaaact agtaatagct gttgaacgca acgcagcaga
    49861 gcaagtgctg gcagcgttac attcccatcc actggggaaa gacgcggcgc taattggtga
    49921 agtggtggaa cgtaaaggtg ttcgtcttgc cggtctgtat ggtgtgaaac gaaccctcga
    49981 tttaccacac gccgaaccgc ttccgcgtat atgctaataa aattctaaat ctcctatagt
    50041 tagtcaatga ccttttgcac cgctttgcgg tgctttcctg gaagaacaaa atgtcatata
    50101 caccgatgag tgatctcgga cagcaagggt tgttcgacat cactcggaca ctattgcagc
    50161 agcccgatct ggcctcgctg tgtgaggctc tttcgcaact ggtaaagcgt tctgcgctcg
    50221 ccgacaacgc ggctattgtg ttgtggcaag cgcagactca acgtgcgtct tattacgcat
    50281 cgcgtgaaaa agacaccccc attaaatatg aagacgaaac tgttctggca cacggtccgg
    50341 tacgccgcat tttgtcgcgc cctgatacgc tgcattgcag ttacgaagaa ttttgtgaaa
    50401 cctggccgca gctggtcgct ggtgggctat acccctaatt tggtcactat tgcctgatgc
    50461 cactggcggc ggaagggcat atttttggtg gctgtgaatt tattcgttat gacgatcgtc
    50521 cctggagcga aaaagagttc aatcgtctgc aaacatttac gcagatcgtt tctgtcgtca
    50581 ccgaacaaat ccagagccgc gtcgtcaaca atgtcgacta tgagttgtta tgccgggagc
    50641 gcgataactt ccgcatcctg gttgccatta ccaacgcggt gctttcccgc ctggatatgg
    50701 acgaactggt cagcgaagtc gccaaagaaa tccattacta tttcgacatt gacgatatca
    50761 gtatcgtctt acgcagccac cgtaaaaaca aactcaacat ctactccact cactatcttg
    50821 ataaacaaca tcccgcccac gaacagagcg aagtcgatga agccggaacc ctcaccgaac
    50881 gcgtgttcaa aagtaaagag atgctgctga tcaatctcca cgagcgggac gatttagccc
    50941 cctatgaacg catgttgttc gacacctggg gcaaccagat tcaaaccttg tgcctgttac
    51001 cgctgatgtc tggcgacacc atgctgggcg tgctgaaact ggcgcaatgc gaagagaaag
    51061 tgtttaccac taccaatctg aatttgctgc gccagattgc cgaacgtgtg gcaatcgctg
    51121 tcgataacgc cctcgcctat caggaaatcc atcgtctgaa agaacggctg gttgatgaaa
    51181 acctcgccct gaccgagcag ctcaacaatg ttgatagtga atttggcgag attattggcc
    51241 gcagcgaagc catgtacagc gtgcttaaac aagttgaaat ggtggcgcaa agtgacagta
    51301 ccgtgctgat cctcggtgaa actggcacgg gtaaagagct gattgcccgc gcgatccata
    51361 atctcagtgg gcgtaataat cgccgcatgg tcaaaatgaa ctgcgcggcg atgcctgccg
    51421 gattgctgga aagcgatctg tttggtcatg agcgtggggc ttttaccggt gccagcgccc
    51481 agcgcattgg tcgttttgaa ctggcggata aaagctccct gttcctcgac gaagtgggcg
    51541 atatgccact ggagttacag ccgaagttgc tgcgtgtatt gcaggaacag gagtttgaac
    51601 gtctcggcag caacaaaatc attcagacgg acgtacgttt aattgccgcg actaaccgcg
    51661 atctgaaaaa aatggtcgcc gaccgagagt tccgtagcga tctctattac cgcctgaacg
    51721 tattcccgat tcacctgccg ccactacgcg agcgtccgga agatattccg ctgctggcga
    51781 aagcctttac cttcaaaatt gcccgtcgtc tggggcgcaa tatcgacagc attccagccg
    51841 agacgctgcg caccttgagc aacatggagt ggccgggtaa cgtacgcgaa ctggaaaacg
    51901 tcattgagcg cgcagtattg ctaacacgcg gcaacgtgct gcagctgtca ttgccagata
    51961 ttgctttacc ggagcctgaa acgccgcctg ccgcaacggt tgtcgctcag gagggcgaag
    52021 atgaatatca gttgattgtt cgcgtgctga aagaaaccaa cggcgtggtt gccgggccta
    52081 aaggcgctgc gcaacgtctg gggctgaaac gcacgaccct gctgtcacgg atgaagcggc
    52141 tgggaattga taaatcggca ttgatttaac tgcaaattgc cggacagatc tgcctgtccg
    52201 gcatactatt cacgaggttt tttcggacga tatttttccg gcagttctgg caccggacgc
    52261 ttgtcatcga tgagatgacg cacggttaag atcggatgac gccacagcat tctcggcccg
    52321 gcccaacgca taatctgttt catctcttca cgctttgcag gctggtaaca gtgcaccgga
    52381 cactgcttac aggctggttt ctcttcgccg aacacacatt tatccagccg cttttgcgcg
    52441 taaacaaaca acgcctcgta atgctccggc tccgctgacg cctgcgggca tttcgcttga
    52501 taaagatcga tcattttttt aatcgtcagt ttttcacgag agatacgctt gccggacatg
    52561 ctgcctccat actcttaagg tgtatttata ttacaactta atttgaaagg ggactatggc
    52621 tctgaaggag gaggtttatt caaccgacgc aattttgcgt acttgcttca taagcatcac
    52681 gcaaaagctg caaaacagca tctttcccgg aaccagcatc aagaactcgc cgttcgcttc
    52741 ttcccctgaa atgattaact ccggtatcat gtgcgcctta tgtgattaca acgaaaataa
    52801 aaaccatcac accccattta atatcaggga accggacata accccatgag tgcaatagaa
    52861 aatttcgacg cccatacgcc catgatgcag cagtatctca ggctgaaagc ccagcatccc
    52921 gagatcctgc tgttttaccg gatgggtgat ttttatgaac tgttttatga cgacgcaaaa
    52981 cgcgcgtcgc aactgctgga tatttcactg accaaacgcg gtgcttcggc gggagagccg
    53041 atcccgatgg cggggattcc ctaccatgcg gtggaaaact atctcgccaa actggtgaat
    53101 cagggagagt ccgttgccat ctgcgaacaa attggcgatc cggcgaccag caaaggtccg
    53161 gttgagcgca aagttgtgcg tatcgttacg ccaggcacca tcagcgatga agccctgtta
    53221 caggagcgtc aggacaacct gctggcggct atctggcagg acagcaaagg tttcggctac
    53281 gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg aaccggccga ccgcgaaacg
    53341 atggcggcag agctgcaacg cactaatccg gcggaactgc tgtatgcaga agatttcgcc
    53401 gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc gcccgctgtg ggagtttgaa
    53461 atcgacaccg cgcgccagca gttgaatctg caatttggca cccgcgatct ggtcggtttt
    53521 ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt gtctgttgca gtatgcgaaa
    53581 gatacccaac gcacgaccct gccgcatatt cgttctatca ccatggaacg tgagcaggac
    53641 agcatcatta tggatgccgc gacacgtcgt aacctggaaa ttactcagaa cctggcgggc
    53701 ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg tcacgccgat gggtagtcgt
    53761 atgctgaaac gctggctgca tatgccagtg cgcgataccc gcgtgttgct tgagcgccag
    53821 caaactattg gcgcattgca ggatttcacc gccgggctac agccggtact gcgtcaggtc
    53881 ggcgaccttg aacgtattct ggcacgtctg gctttacgaa ctgctcgccc acgcgatctg
    53941 gcccgtatgc gccacgcttt ccagcaactg ccggagctgc gtgcacagtt agaaactgtc
    54001 gatagtgcac cggtacaggc gctacgtgag aagatgggcg agtttgccga gctgcgcgat
    54061 ctgctggagc gagcaatcat cgacacaccg ccggtgctgg tacgcgacgg tggtgttatc
    54121 gcatcgggct ataacgaaga gctggatgag tggcgcgcgc tggctgacgg cgcgaccgat
    54181 tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg gcctggacac gctgaaagtt
    54241 ggctttaatg cggtgcacgg ctactacatt caaatcagcc gtgggcaaag ccatctggca
    54301 cccatcaact acatgcgtcg ccagacgctg aaaaacgccg agcgctacat cattccagag
    54361 ctaaaagagt acgaagataa agttctcacc tcaaaaggca aagcactggc actggaaaaa
    54421 cagctttatg aagagctgtt cgacctgctg ttgccgcatc tggaagcgtt gcaacagagc
    54481 gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg cggaacgggc ctataccctg
    54541 aactacacct gcccgacctt cattgataaa ccgggcattc gcattaccga aggtcgccat
    54601 ccggtagttg aacaagtact gaatgagcca tttatcgcta acccgctgaa tctgtcgccg
    54661 cagcgccgta tgttgattat taccggcccg aacatgggcg gtaaaagtac ctatatgcgc
    54721 cagaccgcac tgattgcgct gatggcctac attggcagct atgtaccggc acaaaaagtc
    54781 gagattggac ctatcgatcg catctttacc cgcgtaggcg cggcagatga cctggcttcc
    54841 gggcgctcaa cctttatggt ggagatgact gaaaccgcca atattttaca taacgccacc
    54901 gaatacagtc tggtgttaat ggatgagatc gggcgtggaa cgtccaccta cgatggtctg
    54961 tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga ttaaggcatt gacgctgttt
    55021 gccacccact atttcgagct gacccagcta ccggagaaaa tggaagacgt cgccaacgtg
    55081 catctcgatg cgctggagca cggagacacc attgccttta tgcatagcgt gcaggatggc
    55141 gcggcgagca aaagctacgg cctggcggtt gcagctctgg caggcgtgcc aaaagaggtg
    55201 attaagcgcg cacggcaaaa actgcgtgag ctggaaagca tttcgccgaa cgccgccgct
    55261 acgcaagtgg atggtacgca aatgtctttg ctgtcagtac cggaagaaac ctcgccagcg
    55321 gtcgaagctc tggaaaacct ggacccggat tcactgactc cgcgtcaggc gctggaatgg
    55381 atttatcgct tgaagagcct ggtgtaataa caattcccga tagtcttttg ctatcgggaa
    55441 tattaacgac aactgacgaa taaaataaaa accccctgta taataggaaa gcttatttta
    55501 cagggtaaaa ccatgccatc tacacgctat caaaaaatta atgcccatca ctatcgccat
    55561 atatgggtcg ttggtgatat tcatggtgaa tatcaattat tacaatcccg cttacatcaa
    55621 ctctcttttt gccctgaaac cgacttactt atttctgtcg gcgataatat tgatcgtggg
    55681 ccggagagtc ttgacgtcct gcgcctgcta aaccagccct ggtttatctc cgttaaaggc
    55741 aaccacgaag caatggagct ggatgcgttc gcgaccggcg atggcaatat gtggcttgcc
    55801 agcggtggtg actggttttt cgatttaaat gattcagagc aacaagaggc aatagatctg
    55861 ttgctgaaat tccatcacct tccacatatt attgaaatca ctaacgacat aataaaatat
    55921 gtcatcgcac atgcagatta tccgggtgat gaatatctct ttggcaaaga aatagcggag
    55981 agcgaattac tctggcctgt ttatcgtgtg cagaaatcgc ttaatggcga gttacaacaa
    56041 ataaacggcg ctgattattt tatatttgga catatgatgt ttgataacat tcagacgttc
    56101 gctaaccaga tttatattga taccggatcg ccgaaaagtg ggcggctgtc attttataaa
    56161 ataaggtagt ctcatgcttc ttctgtgaag catgagtaac cagatgttat tgcaggccat
    56221 tattcatttt tcgctaccag caaagagaga tcctgcttcg ccagcgcgcg actggcactc
    56281 tccggcaaac cgtcgtctgt aataatctga tcaaactcgc ttaatggtaa cgccagccat
    56341 gtcgccacct gaccatattt cgtcgcatca cagaccaaaa ctcgctggcg gctggcacta
    56401 gcaatcgccc gtttcaccgt gactttatct tccgctggcg tagaaatccc ccgcacactc
    56461 catgacgatg cagaaataaa agcctgatca atcatcaggc tgcgcagcat ggtcgcagcg
    56521 gcttccccga cacaggaacg gttttcccga cacactgcac cgccagtgtg aataattgtg
    56581 caattactgt tgtcgagcaa gtagttcgca ataacgaaat cgtttgtgac cacagtcagt
    56641 gactccatgt gaatcagatg ctgtgctatc gctaacgtgg tcgttcccgc atccagatag
    56701 atacaacttc ccggctgaac aagacttgcc gccagcttgc caatagccgc tttttgcgtc
    56761 attgctagcg cagtttttac ctgatgagaa ggttcatgcg ccacgcgtcc cggagactgg
    56821 acgcctccgg acaccagcac aacggctccc tgctgctcca gtttttgtaa atcccgacga
    56881 atggtcatat gtgatacatt cattctgtcc gttagttcag caatactgac aatgcctttt
    56941 tcagctacca tctcaaggat gatttggcga cgctctacgg gtatcaactt ttgctccttc
    57001 ctttgtcctg ctgacattct acgctatttg cctgcgaaac gtgcgcggcg caactaacgc
    57061 ttagttcaca taaaataaca cacaatgtta atttatgtga ataggctcac catatcgtta
    57121 tcttccagcg cttatattca caatatcaaa caaaatatca cttaaattaa caaggagagc
    57181 agatgaaaac gggatctgag tttcatgtcg gtatcgttgg cttagggtca atgggaatgg
    57241 gagcagcact gtcatgtgtc cgcgcaggtc tttctacctg gggcgcagac ctgaacagca
    57301 atgcctgcgc tacgttgaaa gaggcaggtg cttgcggggt ttctgataac gccgcgacgt
    57361 ttgccgaaaa actggacgct ctgctggtgc tggtggtcaa tgcggcccag gttaaacagg
    57421 tgctgtttgg tgaaacaggc gttgcacaac atctgaaacc cggtacggca gtaatggttt
    57481 cttccactat cgctagtgct gatgcgcaag aaattgctac cgctctggct ggattcgatc
    57541 tggaaatgct ggatgcgcca gtttctggtg atgcagtaaa agccgctaac ggtgaaatga
    57601 ctgtcatggc ctccggtagc gatattgcct ttgaacgact ggcacccgtg ctggaagccg
    57661 ttgccggaaa agtttatcgc ataggtgcag aaccgggact aggttcgacc gtaaaaatta
    57721 ttcaccagtt gttagcgggc gtacatattg ctgccggagc cgaagcgatg gcacttgcag
    57781 cccgtgcggg gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc gccggaaatt
    57841 cctggatgtt cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc ccgcattcag
    57901 ccgtcgatat ttttgttaag gatcttggtc tggttgccga tacagccaaa gccctgcact
    57961 tcccgctgcc attggcctca acagcattga atatgttcac cagcgccagt aacgcaggtt
    58021 acgggaaaga agacgatagc gcagttatca agattttctc tggcatcact ctaccgggag
    58081 cgaaatcatg atcaagattg gcgttatcgc cgatgatttt accggcgcga cggatatcgc
    58141 cagttttctg gtggaaaacg gtctaccaac ggtacaaatt aacggtgttc caacaggtaa
    58201 aatgccggaa gcaatcgacg cactggtgat cagcctgaaa acgcgctcct gtccggtggt
    58261 tgaagccaca cagcaatcgc tggcggctct gagctggttg caacagcaag gttgcaaaca
    58321 gatctatttc aaatactgct ctactttcga cagtacggcg aaaggtaata tcggcccggt
    58381 taccgatgca ttaatggatg ctctcgacac gccgtttacg gtcttctctc cggccctgtc
    58441 ggtcaacgga cgtacggttt atcaggggta tttgttcgta atgaatcaac tgctggccga
    58501 atccgggatg cgccatcacc cggtaaatcc catgaccgac agctatcttc cccgtctggt
    58561 tgaagcgcaa tccacagggc gctgcggcgt cgtttcggca catgttttcg aacaaggtgt
    58621 ggatgccgtt cgtcaagagc tggctcgctt acagcaagag ggctaccgct acgcggtgct
    58681 tgatgcgctg accgaacacc atctggaaat tcagggagaa gccttgcgcg atgccccact
    58741 ggtaacgggc ggttctggtc tggcgattgg cctggcccgg cagtgggcgc aagaaaacgg
    58801 taaccaggct cgcgaagcag ggcatccgct cgctgggcgc ggcgtagtgc tctccggttc
    58861 atgctctcaa atgaccaacc gccaggtggc acattaccgt caaattgcac cagcccgtga
    58921 agttgatgtg gcacgctgcc tctcaaccga aactctggcc gcttatgcac acgaactggc
    58981 agagtgggtt ctgggccagg aaagtgtact tgctccactg gtttttgcca ccgccagcac
    59041 tgacgcattg gcagcaattc aacagcaata cggtgcacaa aaagccagtc aggcagtaga
    59101 aactctgttt tctcaactag cggcgcggtt agcagcggaa ggcgtgacac gctttattgt
    59161 cgcaggcggt gagacctccg gcgtagtcac acagagcctg ggcataaaag ggtttcatat
    59221 tggcccaacc atttcccccg gcgtgccgtg ggtaaacgca ctggataagc ctgtctcact
    59281 cgcccttaaa tctggcaact tcggtgatga agcctttttt tcacgagccc aaagagagtt
    59341 tttatcatga gcgatttcgc aaaagtagag cagtctttgc gagaggagat gacgcggatt
    59401 gccagttcat tctttcagcg cggctatgca accggttcgg ctggcaatct gtcgctgctt
    59461 ttacctgacg ggaatttact ggcgacaccg acaggttcat gcctgggcaa tctcgatccg
    59521 cagcggcttt ccaaagtcgc cgcggatggc gaatggttaa gtggtgacaa accctcgaaa
    59581 gaggtgctct ttcatctggc gctgtatcgc aacaatccgc gctgtaaagc ggtggtgcat
    59641 ttgcacagca catggtcgac ggtgctttcc tgcctgcaag ggctggacag cagcaacgtt
    59701 attcgtccgt tcacaccata cgtggtgatg cggatgggaa atgtcccgct ggtgccctat
    59761 taccgaccgg gcgataaacg catcgcacag gatctggcgg aactggcagc agacaatcag
    59821 gcttttttac tggcaaatca tggcccagtg gtttgcggtg aaagcctgca agaagccgcc
    59881 aacaatatgg aagagctgga ggaaacggca aagctgattt ttattctcgg tgaccgcccg
    59941 atccgttatc tgaccgcagg tgaaattgcg gaattaagga gttaatgcaa tgcctcgttt
    60001 tgcagctaat ttatccatga tgttcaccga agtgcctttt attgaacgct tcgccgcagc
    60061 gcgaaaagcc gggttcgatg ctgtggaatt tctgtttccc tatgactact ccaccctgca
    60121 aatccaaaag caactggagc aaaaccatct gacactggcg ctgttcaata ccgctcccgg
    60181 agatattaat gcgggggaat ggggattatc cgcccttccc ggacgtgagc acgaagcaca
    60241 cgcagacatc gacctggcac tggaatatgc attggcgctc aattgtgaac aagtccatgt
    60301 gatggcaggc gtcgtgcccg ctggcgaaga tgctgaacgg taccgggcag tatttatcga
    60361 taatctccgc tacgctgccg accgctttgc gccacacggc aagcgaatat tagttgaagc
    60421 actcagcccc gacgttaagc ctcattatct cttctccagc cagtatcagg cactggctat
    60481 tgttgaagag gttgcgcgag ataacgtgtt tattcaacta gatacttttc atgcgcaaaa
    60541 agtagatggc aacctgacgc atttaatccg cgactacgct gggaaatatg cgcacgtaca
    60601 aattgccgga ctacctgatc gacatgaccg gacgatggag aaatcaacta tccgtggctg
    60661 ttccgcctgt tcgatgaggt gggatatcag ggctggatcg gttgtgaata taaacctcgt
    60721 ggcctcaccg aagaagggct tggctggttt gacgcctggc gctaattcgt taaatcccta
    60781 attacaacgt acccatacat ccccccatct acggatgggg taggttaact ataactttca
    60841 gacagggttt cccatgtcca caattacatt gttatgcatt gcgttagctg gcgtaatcat
    60901 gctgttgctg ctggtcatca aggcaaaggt acaaccattc gttgctctgc tcctcgtcag
    60961 cctgttagtc gcacttgcgg cgggtatacc ggcgggcgaa gtgggtaaag tgatgatcgc
    61021 cgggatgggc ggtgtgcttg gttccgtcac tattattatt ggtctgggcg ctatgctggg
    61081 gcgtatgatc gaacactctg gtggtgcaga gtcactggct aattatttca gtcgcaagtt
    61141 aggtgacaaa cgaactatcg ctgcgctgac tctggcagcg ttcttcctcg gtattcccgt
    61201 cttctttgat gtcagcttta ttattcttgc gccaatcatt tacggttttg ccaaggttgc
    61261 caaaatatcg ccactcaaat ttggcctgcc tgtcgctgga atcatgctaa ctgttcatgt
    61321 ggcagtaccg ccgcatccag gccctgtcgc cacagcgggg ttactccacg cagacatcgg
    61381 ctggctaacc atcatcggta ttgcgatttc tattcccgta gggattgttg gctactttgc
    61441 agcgaaaata atcaataagc gccaatatgc gatgtcagta gaagtactgg aacagatgca
    61501 actggctccg gccagtgagg aaggcgcgac aaaattaagc gataaaataa atccaccggg
    61561 cgtcgcgctg gtcacctcgc taattgttat tcctatcgcg attatcatga cgggtacggt
    61621 ttccgcaaca ctgatgccgc cttcgcatcc cctgcttggt acgctacagc tgatcggctc
    61681 accaatggta gctctaatga ttgcgctggt gctggcattc tggttattgg ctttacgtcg
    61741 cggctggagc ttacaacaca ccagcgacat tatgggctca gcccttccca ctgcggcggt
    61801 agtgattttg gttactggtg ctggaggggt atttggcaaa gtgctggtgg aatcgggcgt
    61861 cggcaaagcc cttgccaata tgctacaaat gattgacctg cctctgttac cagccgcatt
    61921 tattatttca ctggctctgc gtgcatcgca ggggtcagca accgtagcaa tcctgacaac
    61981 cggcgggtta ctctcagaag cggtgatggg attgaatccg attcagtgcg tattggtgac
    62041 gctggcagcc tgctttggtg ggcttggcgc gtcacatatt aatgactcag ggttctggat
    62101 tgtgaccaaa tatctggggt tgtcggtagc agacggtctg aaaacctgga cagtgttaac
    62161 gaccattctc ggttttaccg gattcttaat tacctggtgc gtatgggcgg taatttgacc
    62221 ttaataaaaa ggtccgatgg gcatcggacc ttttattgtg cacagaaaag gccagcctcg
    62281 cttgagactg gcctttctga cagatgctta cttactcgcg gaacagcgct tcgatattca
    62341 gcccctgcgt ttgcaggatt tcacgcaaac ggcgcaggcc ttcaacctga atctggcgaa
    62401 cacgttcacg ggtgaggcca atttcacgac ctacatcttc cagtgttgcc gcttcgtacc
    62461 ccagcaaacc gaatcgacgt gccagcactt cacgctgttt ggcgttcagc tcgaacagcc
    62521 atttgacgat gctctgcttc atatcgtcat cttgcgtggt atcttccgga ccgttctctt
    62581 tttcatcggc caggatgtcc agcaacgctt tttcggaatc accacccagc ggggtgtcta
    62641 ccgaggtaat gcgctcgtta agacgaagca tacggctgac gtcatcaact ggcttatcca
    62701 gttgctctgc gatctcttcc gcacttggtt catggtccag cttatgggac aactcacgtg
    62761 cggttcgcag gtaaacgttc agctccttta cgatgtgaat cggcaaacga atagtacggg
    62821 tttggttcat aatcgcccgt tcaatcgtct ggcgaatcca ccaggttgcg tatgttgaga
    62881 agcggaaacc acgttccggg tcaaacttct ctaccgcgcg gatcagcccc aggttgccct
    62941 cttcgataag gtccagcaac gccagaccac gattgccata acggcgggca atttttacca
    63001 ccagacgcaa gttactctcg atcatccggc ggcgagaggc gacatctcca cgcagtgcgc
    63061 gacgcgcaaa ataaacttct tcttcggccg ttaacagtgg tgaataacca atctcaccaa
    63121 ggtaaagctg agtcgcgtcc aacactcgct gtgtggctcc ctgcgataac agttcctctt
    63181 cggccaaatc gttatcactg ggttcctctt ctactaaggc cttttcgtca aaaacctcaa
    63241 ctccgttctc atcaaattcc gcatcttcat ttaaatcatg aactttcagc gtattctgac
    63301 tcataaggtg gctcctaccc gtgatccctt gacggaacat tcaagcaaaa gcctggttcc
    63361 gccgatttat cgctgcggca aataacgcag cgggtttacg gatttcccct tgtaacgaat
    63421 ttcaaaatgc aagcgtgttg aactggttcc ggtgctaccc atggttgcta ttttttgccc
    63481 cgccttcact tcttgttgtt cccggaccag cattgtgtcg ttatgggcgt aggcactcag
    63541 gtaatcatca ttatgtttga tgataatcag attaccgtag ccgcgcagcg cgttaccggc
    63601 ataaacaacg cggccatctg cggtcgcgat aattgcctgt cctttgctgc ctgcgatatc
    63661 aatccccttg ttgcccccct cagaagcgcc aaaggtttcg atcactttgc cctcagtcgg
    63721 ccagcgccag gtggagatag gcgtactggt tgatgtactg ctgacagttg gctcggttgt
    63781 gcttgctgtt ggtaccgtta caggcgctgt gaccgtggtc gcagctggct tgttgttcgg
    63841 caacattttg ttagcactct gttcacccga agattcagaa tacgtaattg tcggttgcga
    63901 cgcaacagca acggtggaat tttgtgcagg cttgatcaca actccttgct ctgctgcgtc
    63961 ggcctgggta atggcatttc cgccagtgat tggcgtaccg gaagcattac ccacctgcaa
    64021 ggtctgacca acgttcagcg cgtatggtgc ctgaatattg ttgcgctgag caaggtcacg
    64081 gaaatcgttg ccagtaatcc aggcgatata gaaaagtgtg tcgccttttt tcacggtata
    64141 ggtactgccg ctataactgc ctttcggaat gttcccatac tgacggttat agacgatgcg
    64201 tccgttttcc atctgtaccg gctgctgagc tactggctgc actggctgga tttgcggttg
    64261 ctgggctgta gacgtcgtcc ccattttcgg cggcggcgta atcaacatac cagaattggt
    64321 atttgcaggc gcattgccat taacggagct gaccggtgcc ggtggatttg aagtgtcaga
    64381 acagcctgcc agccatagcg aaaccagtga caaagccgca atgcggcgaa cggtgaattt
    64441 tgggcttccc gcgctcattt atcccccagg aaaaattggt taataaccag tgacataatt
    64501 accgtgcaag gcaccctact gaacactgga aaagatgttc acgatacgct gacctgcggc
    64561 aaaataacca ggaaaaatcc aggtatttcc tcacgtttta agccagctcc cccttcacta
    64621 aagggacaaa gcgcacggcc tccacggtat cgataataaa ttcgcctccc cgacgacgca
    64681 cccgtttcaa atactggtgc tcctccccta cgggtaagac gagaatcccg ccttcgtcca
    64741 gctgcgtcat tagcgcagtt ggaatttccg gcggtgccgc cgtaacaatg atagcgtcaa
    64801 acggcgcgcg tgcctgccaa ccttgccatc catcgccatg acgggttgaa acattatgta
    64861 aatcaagatt tttcaggcgg cgacgcgcct gccactgcaa acctttaatc cgttcgaccg
    64921 agcaaacatg ctggacaaga tgcgccagga ttgccgtttg atatcccgaa ccggtgccaa
    64981 tttccagcac ccgcgactgc ggcgtcagct cgagtaattc ggtcattcgc gccaccatat
    65041 acggctgcga aattgtctgc ccctgaccta tcggcaaggc gatattgtcc caggcttttt
    65101 gttcaaacgc ttcatcaacg aatttttcac gcggcacggc agcaagtgca ttcagcacct
    65161 gctcatcctg aataccttgc gcacgtaatt gatccagaag tgcttgtacg cgtctgctta
    65221 ccattgcgtg ccaactccca cgctgtttaa ccagtctgaa accacatctt gcgcgctatg
    65281 cgcagttaaa tccacatgca gcggcgtgat ggagacatag ccctcatcta ccgcagcaaa
    65341 atcggtcccc ggaccggcat cacatttacc gcccggcggg ccaatccagt acagcgtatt
    65401 gccgcgcgga tcttgctgcg ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt
    65461 cacgcgaata cctttgattt gatccaaggg taaatccgga acgttaatat taagaatacg
    65521 tccggtgcgc agcggctctt tacacagtgc gcgcaaaatt gaacaggtta ccgccgcggc
    65581 agtgtcgtaa tgtttatgcc cgtcaagcga gacggccagc gccggaaaac ctaaatgacg
    65641 gccttccatc gcggcggcta ccgtaccgga ataaataacg tcatccccca gattcggccc
    65701 ggcgttaatg ccggacacaa caatgtccgg gcgcggacgc atcagagcat tcacgccaag
    65761 atagacgcaa tcggtcgggg ttcccatttg cacagcaata tcaccatttt caaaggtaaa
    65821 cgtgcgcagg gaggattcca gtgtcagaga atttgaagcg ccgctgcggt tacgatcggg
    65881 ggcgaccacc tgaacgtcag caaactcacg caaggctttc gccagcgttt gtataccggg
    65941 tgcatgtacc ccgtcatcat tactcagcaa tatgcgcata atcacctgtt gtgttgataa
    66001 gttccctgac aacgctggtt gcaaaactac ccgccggaag ccagaaacgg atctctacgg
    66061 tgacgtcatc ccaccaattc cagcttaatt gttgcggata cagcagcatc gctctgcgcg
    66121 cggcttcaac tttttcgcgc accagtaaag cttgtaattc agtttctgcg gcgacagctg
    66181 cttgttcgaa tgccagcgct ttacgctgag ttccccattc gccactgccc ggcaatgcgg
    66241 cggttatcat cagctcttta tcgttgacgc gatgctgtaa ttccgccagt tcttcggtgg
    66301 ttgctacaaa ccagctacca cgtccggcta attgtagcgc atcgccgtca acaacttgat
    66361 taacgtctgc ttttttgagg cgctcagcaa caatctgatt aaacaacgca ctgcgggctg
    66421 ccgacaacca aaaactccgt ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc
    66481 gcagcgcgcc ctgcaagttg ctaccgccaa tcccaaaacg ttgggcaccg aagtagttcg
    66541 gtacaccttt tacgcaaata tcgatcagac gttgttcaac gtcatcgcga ttgctcactt
    66601 cgcgtagaac cagggtaaag gcgttacctt tcagcgcgcc taaacgcagc ttgcgcttgt
    66661 gccgcgcata ctccagcacc tggcagcctt ccagttgaaa ggcgctcaga tcgggcattt
    66721 ccttgcctgg cacgcgagcg cataaccact gttccgtaac agcatgtttg tctttttgcc
    66781 cagcgaagct gacttcacgg gcatgaattt tcaggaattt cgccagtgca tccgccacaa
    66841 aacgggtatt gcagccgttt ttgaggattc taaccagaat atgctcacct tcaccatcag
    66901 gctcaaagcc caaatcttcc accaccacaa agtcttccgg attggctttc agcagcccgg
    66961 tgccttgcgg tttaccgtgg aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt
    67021 aatgagtagc gccaccgctt cacaggcaat cccttcccca cgtccggtaa atccaagttt
    67081 ttccgtagta gtggctttca cgttaacatc atccatatgg cagccgagat cttcggcaat
    67141 aaacacgcgc atttgtggaa tgtgcggcaa catcttcggt gcctgagcga tgatagtgac
    67201 atcgacgttg cccagggtat aacccttcgc ctgaatacga cgccaggctt cgcgtagcag
    67261 ctcgcggctg tcggcacctt taaatgccgg atcggtatcc gggaacagct tgccgatatc
    67321 ccccagcgcc gccgcgccaa gcaatgcatc ggtcaacgca tggagcgcca cgtcgccatc
    67381 agaatgcgcc agcaatcctt tttcgtaagg aatgcgtacg ccaccaatga taattgggcc
    67441 ttcaccgcca aaggcatgta cgtcaaaacc gtgtccaatt cgcattatgt attctcctga
    67501 tggatggttc gggtgaggta aaactcggcc agtgccaaat cttccgggcg cgtgacttta
    67561 atgttatccg cacggccttc gaccaactga ggatggaatc cgcaatattc cagcgccgag
    67621 gcttcgtcgg taatagtcgc gccttcattt agagcgcgcg tcagacagtc atgtaacagc
    67681 tcacgaggga aaaattgcgg cgtcagcgcg tgccataagc cgttgcgatc aacggtatga
    67741 gcaatggcat ttttgcccgg ttcggcacgt ttcatcgtat cgcgcactgg tgcggctagg
    67801 atccctcccg tgcggctggt ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc
    67861 agacaaggac gagcggcgtc atgcaccaat acccactgcg cgtcgccagc ggctttcaaa
    67921 cctgccagca cggaatcggc acgctcatca ccgccatcta caacggtgat ttgcggatga
    67981 ttcgccagag gaagttgtgc aaaacggcta tcgccaggac ttatggcaat gacgacacgt
    68041 ttcacccggg gatgcgccag cagcgcatgc accgagtgtt caagaatggt ttgattaccg
    68101 attgagagat attgcttagg acattccgtt tgcattcgac ggccaaatcc ggccgccgga
    68161 accacggcgc aaacatccaa atgagtggtt gccatgttaa ttcccgggct gatttatcga
    68221 ttgttttgcc ccgcagactg tgcgcgcttc gacgcgtcag gcaccagacg ataaaaagtt
    68281 tcgcccggcc tggtcatgct gagttcatta cgcgcacgct cttcgagcgc ctcctggccg
    68341 ccattgagat cgtcaatttc ggcaaaaagt tgatcgtttc gcgctttaag tttcgcgttt
    68401 gtagcttgct gtgccgccac atcatcattg acgcgggtat agtcatgtat accgttctta
    68461 ccgaaccaca gcgaatactg tagccagacc agaatagcca gcaacagcag cgttagttta
    68521 cccatcctgc cccctgaaaa acggcatcat catcccatgc atccgaagac gactctacat
    68581 cctctgttgg ggataccgcg acaacgcggg caaatgtacc acatttgtcc attgttacgt
    68641 atacctaggg cgtgcagaac ataatctcat tattagttac ggtttgaatt atgaacagag
    68701 gagacaagaa agtacaaatt agcccagtag ccacataaac agtgcgccaa acataatgcc
    68761 tactgtcacc agggtgaaaa caatactgta gcgtagcttt ccgtccatca atgaatgcag
    68821 cgcaatcccc accactaccg cgacaggcat cagtgccaga aagaaaggcc aggtgtagat
    68881 aaagaagaac agcgtgttag agccataaat caacatcggc atcgccagcg caaataacca
    68941 ggagataaaa ccgaccacgg caccaggcag tgaccatgtg gtttcttcat cctcagtaag
    69001 gctgtcgtta tttgttagtg taatgttatg gctattacgc atatttgatc ctgttacttt
    69061 gacgaaccgg gcatggaaac ccggtggtgt ctcaggatct gataatatcg ttctgtctca
    69121 acagatctaa taattgctgt accaaatttg ttactaattg ttcaccattg agatgaattt
    69181 ctgccgattc aggcgcttcg taaacggaat ctattcccgt aaagttgcgc agttcaccgg
    69241 cacgcgcttt cttatataag cctttgggat cgcgggcttc gcaaatcgcc agcggcgtat
    69301 cgacaaacac ttcgataaag cgcccttctc ctacgcgttc gcgaaccatc tggcgttcgg
    69361 cgcggtgtgg cgagataaat gcggtcagca ccaccagtcc ggcttcaacc atcaaattcg
    69421 ccacttcacc gacgcgacgg atattctctt tacgatcggc atcgctaaaa ccgagatcgc
    69481 tgcataatcc gtggcgaaca ttgtcgccat ccagcagata cgtactgacg ccgagtttat
    69541 gtaacgcctc ctccagcgcc ccggcgaccg ttgatttacc ggacccagag aggccggtaa
    69601 accacagcac tacaccacga tgaccgtggt gtagctcgcg ttgttgcaca gtgaccggat
    69661 ggctatgcca gacgacgttt tcgtcatgca gcgccattat ttatccccca gcaaatcgcg
    69721 cgcgccccag tgcggaaagt ggcgacgaac cagggcattc aattccagtt cgaatgcact
    69781 gaattcagat gacgcaacgg cggcctcgtt tttcggctca tgcaccatac cggcaccaac
    69841 agtcacgttg ctcaggcgat cgataaaaat cagcccaccc gtcaccggat tttgttgata
    69901 acgatctaac accagcggct cgtcaaaagt gagatccacg aggccgatcc cattcagtgg
    69961 caggttttca acttcacgct gggtaaggtt attaatatca accggatagc gaatgccatc
    70021 aacacgcgcg cgcgtcttct taccggcaat tttgatgtcg taactctggc ccggggaaag
    70081 cggctgttcc gccatccata ccacatccac cgacgcgctc ggcaccgccg gtaacgcttc
    70141 gtctgccgcc agcagcagat cgccacggct gatgtcgatc tcatccgtca gcaccagggt
    70201 gatcgcttct ccggcaaagg cttcttcgcg atcaccatca aaagtcacga tccgcgcgac
    70261 gtttgattcc acaccagagg gcagcgcttt gacacgttgc ccgacttcca cgcgaccgga
    70321 tgccagcgtt ccggcgtagc cacgaaaatc gagattcggg cgattaacgt actgcaccgg
    70381 gaagcgcatt ggctgggcat ccaccactcg ctggatctcc acggtttcca gcacttcgag
    70441 cagtgtcgga ccgctgtacc acgacatact ttcactttgc gatgccacgt tgtcgccttc
    70501 cagtgcagag agcggcacaa agcggatatc cagattaccc ggcagctgcc cggcaaaggt
    70561 caaataatct tcacgaatat gggtgaacgt ctcttcactg taatccacca gatccatttt
    70621 gttgatcgcc acgaccagat gtttgatccc caacagtgtg gagataaaac tgtgacgacg
    70681 ggtttgatcg agcacgcctt tacgggcatc gatcagtaag atcgccagtt cacatgtcga
    70741 tgcgccagtt gccatattgc gagtgtactg ctcgtgccct ggggtgtcgg cgataataaa
    70801 tttacgcttc tcggtagaga aataacggta agccacgtca atggtgatgc cctgttcgcg
    70861 ctcagcttgc aggccgtcca ccagcagtgc cagatccagc ttttcgccct gggtgccgtg
    70921 acgcttactg tcgttatgca gcgatgagag ctgatcttcg tagatttggc gggtatcgtg
    70981 cagcaggcga ccaatcaggg tacttttgcc gtcatcgacg ctaccacagg tcagaaaacg
    71041 cagcaggctt ttatgttgtt gcgcaatcat ccaggcttcg acgccgcctt cattggcgat
    71101 ttgttgtgca agtgcggtgt tcatcttaaa aatacccctg acgttttttc agctccatag
    71161 accccgcctg gtcgcggtca atcacgcggc cctgacgttc actggtggta gaaaccagca
    71221 tctcttcgat gatctccggc agtgtttgtg catttgactc caccgcaccg gtcagcggcc
    71281 agcagcccag cgtacggaaa cgcaccatcc gttttttaat cacttcgccc ggttgcaggt
    71341 cgatacggtt gtcatcaatc atcatcaaca taccgtcgcg ttccagaacc ggacgttccg
    71401 cagcgagata cagtggcaca atgtcgatat tttccagcca gatgtattgc cagatatcct
    71461 gctcggtcca gttagagagc gggaagacgc ggatgctttc acctttgtta atttgcccgt
    71521 tgtagttgtg ccacagctcc gggcgctgat ttttcggatc ccagcgatgg aagcggtcac
    71581 ggaaagagta gattcgctct ttagcgcggg atttctcttc atcacggcgc gcaccaccga
    71641 aggcggcatc aaaaccgtat ttgttcagcg cctgtttcag gccttcagtt ttcataatat
    71701 cggtatgttt cgcgctgccg tgtacgaatg gattaatccc catcgccacg ccttccgggt
    71761 ttttatgcac cagcagctcg cagccgtagg ctttagcagt acgatcgcgg aactcataca
    71821 tctcgcggaa tttccagccg gtatcgacat gcagcaacgg gaaaggcagc gtacctggat
    71881 aaaacgcctt gcgcgccaga tgcagcatga cgctggaatc tttaccgata gagtagagca
    71941 tcaccggatt tgagaattct gccgccacct cgcgaataat gtggatgctt tccgcctcca
    72001 gttgccgcag gtgagtaagt cgtttttgag ccataaccgt tcctttgcaa taccgctatt
    72061 ttcttgccat cagatgtttc gactataggg agcgtaagag aacgaatgaa attaccaatt
    72121 agaatgagta gttccttaac ggaataacga tttggcaaag ctaatatcaa aaagtgctta
    72181 aggcaccgga tttcgggcgt ttaggaagat ttgaaattgt tttagcgcag cggcagtttc
    72241 atactatggc ggtaaaaaaa tttgcatggt atttaaggac tcactatgtt ttccgcattg
    72301 cgccaccgta ccgctgccct ggcgctcggc gtatgcttta ttctccccgt acacgcctcg
    72361 tcacctaaac ctggcgattt tgctaatact caggcacgac atattgctac tttctttccg
    72421 ggacgcatga ccggaactcc tgcagaaatg ttatctgccg attatattcg ccaacagttt
    72481 cagcaaatgg gttatcgcag tgatattcgg acatttaata gtcggtatat ttataccgcc
    72541 cgcgataatc gtaagagctg gcataacgtg acgggaagta cggtgattgc cgctcatgaa
    72601 ggaaaagcgc cgcagcagat catcattatg gcgcatctgg atacttacgc cccgctgagc
    72661 gatgctgacg ccgatgccaa tctcggcggg ctgacgttac aaggaatgga tgataacgcc
    72721 gcaggtttag gtgtcatgct ggaattggca gaacgcctga aaaatacgcc taccgagtat
    72781 ggtattcgat ttgtggcgac cagcggcgaa gaggaaggga aattaggcgc tgagaattta
    72841 ctcaagcgga tgagtgacac cgaaaagaaa aatacgctgc tggtgattaa tctcgataac
    72901 ttaattgttg gcgataaatt gtatttcaac agcggtgtaa aaaccactga agcagtaagg
    72961 aaattaacgc gcgacagggc gctggcaatt gcgcgcagtc acggaatagc cgcaacgacc
    73021 aatccggggt tgaataaaaa ttatccgaaa ggcactgggt gttgtaatga cgcagaaata
    73081 ttcgacaaag cgggcattgc tgtactttcg gtggaagcga ctaactggaa tcttgggaat
    73141 aaggatggtt atcagcaacg cgcaaaaaca gccgcattcc ctgcgggaaa tagctggcat
    73201 gacgtaagac tggataatca acagcatatt gataaggcac ttcctggaag aatagaacgt
    73261 cgctgccgtg acgttatgcg gataatgcta ccgttggtga aggagctggc gaaggcgtct
    73321 tgatgggttt gaaaatggga gctgggagtt ctaccgcagg ggcggggaat tctaagtgat
    73381 atccattatc gcatccagtg cgcccggttt atccccgctg gcgcggggaa cacactaagc
    73441 atacataact gtttttaaac aaatttattc cgcatcaaca atctaccaac taaattaaaa
    73501 catttcctta tttttaaaga acggataact tattgattat caacaggaag aaaagaaacc
    73561 aaacgtaacc catccaaatc caccggaata cgtctgtttt ctccccaggt ctgaaattca
    73621 aaacccgact cggtattggt cgcccaggcc atcaccacat ttccgcaacc agccagttgg
    73681 gtaatttgct gccagatcat ctcccgaata cgttttgatg tatcaccaac atacacaccg
    73741 gcacgcactt ccagtagcca gattgcgagc cgtccacgta agcgtggcgg gacattttct
    73801 gtaacaacca cgaccatgct catcagccgc gtcctcgatg accgctatcg cccaacgttt
    73861 caggttcggg gatggctggc ggtaacatat ccggcgcagg ttgtggcggt tcaatttcac
    73921 ctgcggcaag gacttcctca attaacggta ttaatttgcc cgttaactta gtgctacgga
    73981 aaatatcacg acaggctaat ctgacttctt tatcaggttc tgcgggttgc ctcgccgcta
    74041 tttcaaatgc ctttggtaca accgaatcaa atttaatgat atcggctatg tcataaacaa
    74101 atgaaagcgg tttgccacta tggataaacc caatagcggg cgcatatccc gcggctaata
    74161 ctgccgcttc agatataccg tacagacatg atgtagcagc actgatgcag cgattcacaa
    74221 catcgccttt ttcccagtct ttaggatcgt atttgcgacc attccatttc acaccatatt
    74281 gtttcgccag taatgcatac gtctggcgaa cgcgagatcc ctcaattcca cgtagctgct
    74341 ccactgaacg gcgagctggc ggtggctcac gaaaacgtaa ttcatacatt ttgcgcacca
    74401 ccttcagacg tagatcttcc gttaaagcca gctttgcctg gtagagtaac ttatcagcac
    74461 gcgccccacc gggttgtccg gaagagtaga cgcgaacacc cgcttcaccg acccagacct
    74521 gtagcgtccc caccgtggcg gccagatgca ccgccgcgtg ggaaactctc gttcccggtt
    74581 cgagcataat gcaggcgacc gatcccaccg gaatgtgcgt gcggatcccg gttttatcga
    74641 tcagcacgaa agcgccgtcc agtacgtcga tttgcccgta ctggaggaag atcatagagg
    74701 tgcgatcttt taacgggatc gggctcagcg gtacaaacgt cacgcctctg ctccgggttt
    74761 gatcagcatc agaccacagc cgaacgcgcg gcttttgcca tacccctgac tgagacgttg
    74821 cagaaataac ccgggatcgg tgaccgtaag catccccgta taatccacgc tactgaactg
    74881 gatcagttgc cgggagtttt cccgccgcag ttgctgttgt cggtaggcat caaccgagat
    74941 atccagcagt gtaaaaccac ttctctcccc ctgcgctgcc agccagtcca gcgccgcctg
    75001 ttgttgatgc aaccagacat cacttccttc cgcctgcccc ctcacctgcc gtttcgcctc
    75061 catcagcaga tcgtggcgct tgcccgcttt acagattgtt ggattcgccc gcaggttgaa
    75121 acaaagttgt tgtccggtac acagctcggg cgcaaatgac cggcattcta tggtgaacgt
    75181 atcactttcc gccgggcgtt cctgcgacag tacaaaaaag cgaaacgcgc cctggagttc
    75241 ttcccgacga taaagaaatt gcctttcttt gccgctaggg aagagatccc acagccactg
    75301 atgcatcaca tattcaccgc gatccaccag atgcagcaac tgcgcaggcg aaagctggcc
    75361 ggtatgtaag gttattcttg agaggtacat ggttcctcct tgctgagcca cggcccctga
    75421 ttgacggtgc gttccccaaa cagccactgc tgacgattta aaggaacatc ccggcggcgt
    75481 aatattttgc tcgcgaccag gccgtcgtgt ttcccttccc accagcattc atcctgaagt
    75541 ttcgggagtg aaactttcag tttatggaaa tgatcctgat actgttggta tgcattacat
    75601 aaggcatcac acgcgttgcc ttcgagcagt aacggcgcaa gaggtaatgc cagcggatga
    75661 ctttttcgcc ccagataaag cggaaaaact ggatggcgta aaccgtcctg caactgttcg
    75721 aggctgtaag gcgcatcggc tgttgttgcc accgccacca tccaccaggc atcggtgtag
    75781 tagtcgcgcc gggagataat cgcgctcaaa agctcagggt cgctcaactc ttcgcgacgg
    75841 ctgaaataac gtgctttacg cacctctttt ggcatctgga ccgtgtgata atcccgtgcc
    75901 cagcgcgggt tacggctggc gcaaaccacc agtgaatagt ggcgattaaa cgcgtttaag
    75961 cgttcggtat catcacgccg gatccctacc ccggcagcca gcagccccag caatgctgag
    76021 agcgaaggca gttcatgggt atgacgcact tcgccggggg catcgacgcc ccaggatgcc
    76081 atcggcccat gaagctgaaa aatcagatat tggctcataa gccgcccctt acgcgcagat
    76141 aaagtccagc acgtccttca tgcttccctg tttgttcatc acgtcaaaac ttgcgtaatc
    76201 ggtcttctgt tcgtagaccg tattcatatt ttcgcgaaga gttgtaatac gctgcaccgc
    76261 cacctctaac tgacgggtgc cattaatggg ttcatagaaa gctgccgcca gagaacgcgg
    76321 ttgatcggtg cctttttctg ccagcgccca ggaggcataa gcccggctgg caaagctgtt
    76381 ttgtttaccg gttggggaga ctttaagcgc ggcttccgta aaggcgcgta tggtctgatt
    76441 agctaacgct tcgtcaccgc cgaggttttc taccagcaga tctttatcaa tgcagataag
    76501 atataggtgt agaacagcgc agaaccgaat ccggtttcat ctacatgacc ggcaccggca
    76561 tcttcagaag cctggcgtaa atcatctacg gcggtgaaaa aatcattttc gacaatcgtt
    76621 tcactgacac caaacgcatg cgcgacctgg caggcggctt ctacattaaa cccgggtttt
    76681 ttcgccagca tacgaccaaa catagcgata tctaccgcca tacggtcttt acgtaacaaa
    76741 gcgagatctt tctcttttgg cgcgcgcttt tcttctgcca gttgatgggc cagcgctttt
    76801 acggcgtcaa attctgccgg gctgatatgg acgagctgtt cagtttcggc gctagtgagc
    76861 ggatcttttg gttttttgtc gtttttttgt tttttgtctt ttttggcttt cccaagataa
    76921 tcggctatct ctactgccca ttcgatggct tttttatctt cgattccttt ctcaatcagg
    76981 atagttgccg cctcacgcgc aatacgcccg ctacgaacac caatatggcc cgccagcgcc
    77041 tgttcaaaaa gttcagaagt gcgccaagca cgcttcagac tttgcgagga aacgcgcaga
    77101 cgcgttgctc cacccagaat cacagttttc ggagagccgg aatcatcacg gttaagattg
    77161 gcagcagggt aagcggttaa caaatgaagc tgaataaatg ttgtcatgag atagtccttt
    77221 attaattttg ttcgttatcg gcgtcgccag cctggtaata ttccagcgcc cagcgcccag
    77281 cgcccagcgc ccagcggata cggataaatt ctgttggtcg ctgctggcgg cggtgatgat
    77341 tgagcaggtc atcgctctcc tggcaccagc ggaagacccc ctcttccaga gagtcaagat
    77401 tgacggaacc gtttaataat ttgaccgcac ggcgtaactg ccgcaataaa tcatccggcg
    77461 tttttactgc caacaaacga gtaaaccgcc cctttttcat taccgccgcc agttgcgcgg
    77521 caaaaggctg ccgttcgtca atagctttaa cattggcgct aactgctgct atcagcgcta
    77581 gtgcggtgat acgccactcg ggttcatcct gccactttat ttgtctgttc ttaagaaaca
    77641 ggcgaaatcc atccgtcaga caaacatcat taaccgtcgt actacgccgc agactggcac
    77701 gctcgccgcg tttatcctgc aactcttcat gccatttacg cagcgcaact ttatgctcat
    77761 cttttacaat actcattcag cagcctcctg ctttttctcc ctggcggcgt tagcattttg
    77821 cttctccgct gatgttgtaa aatatttctt gcgtgcggtc atgacgcgtt ccaaatcaac
    77881 gggctcatat ggattggtaa agacacgatc gtcaaaatcc tgacgcgcga ataaccacat
    77941 ttccttttgc catttactga gtaattcgtc cggatcctga ccttcttcaa ttttgcgcac
    78001 taatctcagg aatcgatgct gagttttgtt ccagaagtca atatccacaa aaccgaaatc
    78061 aagctttgtg gttttcttct cagagaacca tgcttctttc aacgctcggt gtaacaagct
    78121 tagaatgtgt gatgccgctt gcgctgccag acgcaactta ggtattaggt cttctttttt
    78181 gtttaacaac agcgggaaat ggtgttcata ccaacaacga accttcatat cttcaaaatc
    78241 aaaaccaaat ccccataagc ccacccttgt ctgctttaga ttgctggcat tcaaaagttt
    78301 cacgacctgt gcggggagtt cagtattgtt ttttgagtta cctacctcta tgaggcctaa
    78361 ccagtcgcgc cagattaaac caccaggttg tggtttaact gagtaaaaat ctccaccctc
    78421 tttaagcggc agacggtaag gtgttagagg atggcgccac atgacgtatt gaacaccata
    78481 gtttttgagt gacatcaagc ccaaaagagc atcgctctgc ctaccgcaaa tgtcgcagtt
    78541 acctattgag gttgtcttaa aatcaatacg tatacgtcgt ggcattcccc agtaggcctg
    78601 aagtttattg acttgttcat ccgttaccac agcaccgtcc agttcgctgg tacgcgtcgg
    78661 tgcaagccaa ggaaagacca ggtcatcaaa ggtttttggt aaaggtaaat cggcttcatc
    78721 ttgcggcatt acgtttagcc acagctttcg ccacaaaggg gtttgttgat tgccctgata
    78781 ctccagcaat tctataagtg tcgtcagagg ccctccgcca cgtagcccag gatagtaacc
    78841 gcgtccacct ataggtgcat tcaactggat tgaaaaaagc gctaaaggca cacaatgcag
    78901 gcacagatgc tgagttgtgt ctcgcttaat aaaatggtct gtatttcgtt cttttgtttg
    78961 ttttcctggt gcgtcaggaa gaagcgaagc aataaaagtt gccttaactt ttaattcatc
    79021 aaaatcctgc ataaatgaag gggaatctgg cccaaactga aaagcatgtt ctaaagacag
    79081 caatgcttcc cttagctttt cagcttccag tccgtcttcc cagatatcat cccaactacg
    79141 ttgatctttt ggcgcgaagc tggtttgtag tagccccagc aaaaactgcc aggccgcccc
    79201 ctggagatct gcccgcggcg cagcgatatc gacaacattt tcatcagcca gatcgactgg
    79261 tgccagtttg cctgtcgttc cgtctttaaa acgaacgggc aaccacgggg ttgtcagaag
    79321 tgaaaacgag ttcatacgca tcctctcgta aaaaagacca tcccaggtcg gttgtccatc
    79381 cttccatcct tttctgtata tcaactattc agagagtctt gtcgcagaca tctcaaccaa
    79441 ccttgccaat caatccctcc ctggccgaat acccgcaact ttcgtcatcg gtgactaaaa
    79501 tcacgtttgc catttccgga tcttgccgct gttcaacgca ccactgcctg aacgcatccc
    79561 cttccagtaa agaaaactca tcccgatgtt ttttccacca gcttcggcgc actctgacaa
    79621 cgctcatttc ccatgcgtga gcaccggtgg cataaggctt caccacaccg tcaatacagg
    79681 tagccagcca cagggaaaca gactcttcag ccagacgtgt cgacagtttt tccggaaggt
    79741 aatcgttgat attggcggca tagccgagcc tgaagttcag cacatacttt ttagcccttg
    79801 cgcgatcaca gtaatatttg cccacttgct cctgctcgct gcgggcaaat ccttccggca
    79861 ttaccacgtc ctcaccgtaa actgattcaa taagaagccg ggctgcgtgc ggcatctgaa
    79921 tagcgccttg ctcacgcagc acacgctgcg tcagccagat tcgcccatga tcgggataga
    79981 catatgcact gttacgcatg gcactgccga accattcgtc accaggagcg tcatcccaga
    80041 cgggggccag aatcagcaat tcaggggggg aacgctcgtc ttttccgtca cgctttaact
    80101 gaccattaat atcgcggata tgccgctgta atcgccccgc tcgctggatt agcaaatcaa
    80161 tgggggccag gtcggagatc atttcgtcca ggtcaatatc cacgctttgt tcaattactt
    80221 gtgtacaaac aatgacttga gacgctctct gtaaactaca atatttacca aagcgtgcca
    80281 gcgtttccgt ttcaattcgc tggcgatcgc taaaagcaaa gcggctatga aaaagtgaaa
    80341 ggctggaagc aggaatgacg ccgcgggcaa gcagctgacg atacacctga atagcgtcat
    80401 caacagaatt ccgaatccag gcgatgcatt tcccctgact taccgccgat tcgatacgcg
    80461 caatacactc ttgttcacta tggagccaac ccacgctcac gctacgttca acttctttgc
    80521 gcgtcgctac ccggtgcgaa tgtacatcgg ttttcgtgac atgcgtcagc caggggtaat
    80581 catcttttcc caggagtggg gcttcttgct ggccctctgc accacgcgca aaggcggcga
    80641 cgagtttgtc gcgctgctgt tgagataacg tagcagaaag caaaataacg ctgtttccgc
    80701 cacgcgcctg ccgctcgatc agcccttcaa gaatgcacga catataagcg tcacaggcgt
    80761 ggatctcatc agccagcagg attttgttac tcagccccag aagccgcaga ttattatgtt
    80821 taaacggcat cactgccatc atcgcctgat ccagggtgcc gacgccaatt tcagccagca
    80881 acgccttctt gttattgtcg gcaaactagg ccgcacaacc ctggctgaaa gcttgttcat
    80941 ccggttcttc tgacccgatt aaatcaccgg accagagtga ttcatta
//
LOCUS       DAJVOY010000003        66157 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVOY010000003 DAJVOY010000000
VERSION     DAJVOY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.003"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..416)
                     /gene="folK"
                     /locus_tag="OQL90_000178"
     CDS             complement(<1..416)
                     /gene="folK"
                     /locus_tag="OQL90_000178"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1367081.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFP"
     gene            complement(413..1831)
                     /gene="pcnB"
                     /locus_tag="OQL90_000179"
     CDS             complement(413..1831)
                     /gene="pcnB"
                     /locus_tag="OQL90_000179"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1367082.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1870..2796)
                     /gene="gluQRS"
                     /locus_tag="OQL90_000180"
     CDS             complement(1870..2796)
                     /gene="gluQRS"
                     /locus_tag="OQL90_000180"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1367083.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(2833..3288)
                     /gene="dksA"
                     /locus_tag="OQL90_000181"
     CDS             complement(2833..3288)
                     /gene="dksA"
                     /locus_tag="OQL90_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1367084.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3466..4170)
                     /gene="sfsA"
                     /locus_tag="OQL90_000182"
     CDS             complement(3466..4170)
                     /gene="sfsA"
                     /locus_tag="OQL90_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1367085.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4185..4715)
                     /gene="thpR"
                     /locus_tag="OQL90_000183"
     CDS             complement(4185..4715)
                     /gene="thpR"
                     /locus_tag="OQL90_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1367086.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4789..7218
                     /gene="hrpB"
                     /locus_tag="OQL90_000184"
     CDS             4789..7218
                     /gene="hrpB"
                     /locus_tag="OQL90_000184"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1367087.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7414..9936
                     /gene="mrcB"
                     /locus_tag="OQL90_000185"
     CDS             7414..9936
                     /gene="mrcB"
                     /locus_tag="OQL90_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1367088.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10156..12345
                     /gene="fhuA"
                     /locus_tag="OQL90_000186"
     CDS             10156..12345
                     /gene="fhuA"
                     /locus_tag="OQL90_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1367089.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12396..13193
                     /gene="fhuC"
                     /locus_tag="OQL90_000187"
     CDS             12396..13193
                     /gene="fhuC"
                     /locus_tag="OQL90_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1367090.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13193..14083
                     /gene="fhuD"
                     /locus_tag="OQL90_000188"
     CDS             13193..14083
                     /gene="fhuD"
                     /locus_tag="OQL90_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1367091.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14080..16062
                     /gene="fhuB"
                     /locus_tag="OQL90_000189"
     CDS             14080..16062
                     /gene="fhuB"
                     /locus_tag="OQL90_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1367092.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16097..17377)
                     /gene="hemL"
                     /locus_tag="OQL90_000190"
     CDS             complement(16097..17377)
                     /gene="hemL"
                     /locus_tag="OQL90_000190"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1367093.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17602..19023
                     /gene="clcA"
                     /locus_tag="OQL90_000191"
     CDS             17602..19023
                     /gene="clcA"
                     /locus_tag="OQL90_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1367094.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19047..19112
                     /locus_tag="OQL90_000192"
     CDS             19047..19112
                     /locus_tag="OQL90_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367095.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19105..19449
                     /gene="erpA"
                     /locus_tag="OQL90_000193"
     CDS             19105..19449
                     /gene="erpA"
                     /locus_tag="OQL90_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1367096.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19496..20119)
                     /locus_tag="OQL90_000194"
     CDS             complement(19496..20119)
                     /locus_tag="OQL90_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1367097.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20157..20956)
                     /gene="btuF"
                     /locus_tag="OQL90_000195"
                     /pseudo
     CDS             complement(20157..20956)
                     /gene="btuF"
                     /locus_tag="OQL90_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(20949..21647)
                     /gene="mtnN"
                     /locus_tag="OQL90_000196"
     CDS             complement(20949..21647)
                     /gene="mtnN"
                     /locus_tag="OQL90_000196"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1367098.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFEADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21731..23248
                     /gene="dgt"
                     /locus_tag="OQL90_000197"
     CDS             21731..23248
                     /gene="dgt"
                     /locus_tag="OQL90_000197"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1367099.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23378..24802
                     /gene="degP"
                     /locus_tag="OQL90_000198"
     CDS             23378..24802
                     /gene="degP"
                     /locus_tag="OQL90_000198"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1367100.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24957..26114
                     /gene="cdaR"
                     /locus_tag="OQL90_000199"
     CDS             24957..26114
                     /gene="cdaR"
                     /locus_tag="OQL90_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCS1367101.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26203..26589)
                     /locus_tag="OQL90_000200"
     CDS             complement(26203..26589)
                     /locus_tag="OQL90_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1367102.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26751..27563)
                     /gene="yaeI"
                     /locus_tag="OQL90_000201"
     CDS             complement(26751..27563)
                     /gene="yaeI"
                     /locus_tag="OQL90_000201"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCS1367103.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27618..28442)
                     /gene="dapD"
                     /locus_tag="OQL90_000202"
     CDS             complement(27618..28442)
                     /gene="dapD"
                     /locus_tag="OQL90_000202"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1367104.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28473..31145)
                     /gene="glnD"
                     /locus_tag="OQL90_000203"
     CDS             complement(28473..31145)
                     /gene="glnD"
                     /locus_tag="OQL90_000203"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1367105.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31207..32001)
                     /gene="map"
                     /locus_tag="OQL90_000204"
     CDS             complement(31207..32001)
                     /gene="map"
                     /locus_tag="OQL90_000204"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1367106.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32369..33094
                     /gene="rpsB"
                     /locus_tag="OQL90_000205"
     CDS             32369..33094
                     /gene="rpsB"
                     /locus_tag="OQL90_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1367107.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33352..34203
                     /gene="tsf"
                     /locus_tag="OQL90_000206"
     CDS             33352..34203
                     /gene="tsf"
                     /locus_tag="OQL90_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1367108.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34350..35075
                     /gene="pyrH"
                     /locus_tag="OQL90_000207"
     CDS             34350..35075
                     /gene="pyrH"
                     /locus_tag="OQL90_000207"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1367109.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35367..35924
                     /gene="frr"
                     /locus_tag="OQL90_000208"
     CDS             35367..35924
                     /gene="frr"
                     /locus_tag="OQL90_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1367110.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36016..37212
                     /gene="ispC"
                     /locus_tag="OQL90_000209"
     CDS             36016..37212
                     /gene="ispC"
                     /locus_tag="OQL90_000209"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1367111.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37400..38158
                     /gene="ispU"
                     /locus_tag="OQL90_000210"
                     /gene_synonym="uppS"
     CDS             37400..38158
                     /gene="ispU"
                     /locus_tag="OQL90_000210"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1367112.1"
                     /translation="MLSATQLLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38171..39028
                     /gene="cdsA"
                     /locus_tag="OQL90_000211"
     CDS             38171..39028
                     /gene="cdsA"
                     /locus_tag="OQL90_000211"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1367113.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39040..40392
                     /gene="rseP"
                     /locus_tag="OQL90_000212"
     CDS             39040..40392
                     /gene="rseP"
                     /locus_tag="OQL90_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1367114.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40422..42854
                     /gene="bamA"
                     /locus_tag="OQL90_000213"
     CDS             40422..42854
                     /gene="bamA"
                     /locus_tag="OQL90_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1367115.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42976..43461
                     /gene="skp"
                     /locus_tag="OQL90_000214"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42976..43461
                     /gene="skp"
                     /locus_tag="OQL90_000214"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1367116.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43465..44490
                     /gene="lpxD"
                     /locus_tag="OQL90_000215"
     CDS             43465..44490
                     /gene="lpxD"
                     /locus_tag="OQL90_000215"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1367117.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44595..45050
                     /gene="fabZ"
                     /locus_tag="OQL90_000216"
     CDS             44595..45050
                     /gene="fabZ"
                     /locus_tag="OQL90_000216"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1367118.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45054..45842
                     /gene="lpxA"
                     /locus_tag="OQL90_000217"
     CDS             45054..45842
                     /gene="lpxA"
                     /locus_tag="OQL90_000217"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1367119.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            45842..46990
                     /gene="lpxB"
                     /locus_tag="OQL90_000218"
     CDS             45842..46990
                     /gene="lpxB"
                     /locus_tag="OQL90_000218"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1367120.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVARDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46987..47583
                     /gene="rnhB"
                     /locus_tag="OQL90_000219"
     CDS             46987..47583
                     /gene="rnhB"
                     /locus_tag="OQL90_000219"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1367121.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47620..51102
                     /gene="dnaE"
                     /locus_tag="OQL90_000220"
     CDS             47620..51102
                     /gene="dnaE"
                     /locus_tag="OQL90_000220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1367122.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51115..52074
                     /gene="accA"
                     /locus_tag="OQL90_000221"
     CDS             51115..52074
                     /gene="accA"
                     /locus_tag="OQL90_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1367123.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52173..54282
                     /gene="ldcC"
                     /locus_tag="OQL90_000222"
                     /pseudo
     CDS             52173..54282
                     /gene="ldcC"
                     /locus_tag="OQL90_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            54339..54728
                     /locus_tag="OQL90_000223"
     CDS             54339..54728
                     /locus_tag="OQL90_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1367124.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54793..56091
                     /gene="tilS"
                     /locus_tag="OQL90_000224"
     CDS             54793..56091
                     /gene="tilS"
                     /locus_tag="OQL90_000224"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1367125.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56140..56400)
                     /gene="rof"
                     /locus_tag="OQL90_000225"
     CDS             complement(56140..56400)
                     /gene="rof"
                     /locus_tag="OQL90_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1367126.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56387..56587)
                     /locus_tag="OQL90_000226"
     CDS             complement(56387..56587)
                     /locus_tag="OQL90_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1367127.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56753..57298
                     /locus_tag="OQL90_000227"
     CDS             56753..57298
                     /locus_tag="OQL90_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1367128.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57295..57717
                     /gene="arfB"
                     /locus_tag="OQL90_000228"
     CDS             57295..57717
                     /gene="arfB"
                     /locus_tag="OQL90_000228"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1367129.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            57731..58441
                     /gene="nlpE"
                     /locus_tag="OQL90_000229"
                     /gene_synonym="cutF"
     CDS             57731..58441
                     /gene="nlpE"
                     /locus_tag="OQL90_000229"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1367130.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58641..59461)
                     /locus_tag="OQL90_000230"
                     /pseudo
     CDS             complement(58641..59461)
                     /locus_tag="OQL90_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59514..61232)
                     /gene="proS"
                     /locus_tag="OQL90_000231"
     CDS             complement(59514..61232)
                     /gene="proS"
                     /locus_tag="OQL90_000231"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1367131.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61343..62050)
                     /gene="tsaA"
                     /locus_tag="OQL90_000232"
     CDS             complement(61343..62050)
                     /gene="tsaA"
                     /locus_tag="OQL90_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1367132.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62047..62451)
                     /gene="rcsF"
                     /locus_tag="OQL90_000233"
     CDS             complement(62047..62451)
                     /gene="rcsF"
                     /locus_tag="OQL90_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1367133.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62569..63384)
                     /gene="metQ"
                     /locus_tag="OQL90_000234"
     CDS             complement(62569..63384)
                     /gene="metQ"
                     /locus_tag="OQL90_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1367134.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63424..64077)
                     /gene="metI"
                     /locus_tag="OQL90_000235"
     CDS             complement(63424..64077)
                     /gene="metI"
                     /locus_tag="OQL90_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1367135.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64070..65101)
                     /gene="metN"
                     /locus_tag="OQL90_000236"
     CDS             complement(64070..65101)
                     /gene="metN"
                     /locus_tag="OQL90_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1367136.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65289..65861
                     /gene="gmhB"
                     /locus_tag="OQL90_000237"
     CDS             65289..65861
                     /gene="gmhB"
                     /locus_tag="OQL90_000237"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1367137.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15696 a        16411 c        18177 g        15873 t
ORIGIN      
        1 ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc acgattcttc
       61 atatcgtagt gcggaacggt caggcgttcg gtatttatca cttcattacc aaacagcatg
      121 atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg atcctgttgc
      181 agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgagcgaagt ttcaagcgcc
      241 acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggagt gcggtaaaac
      301 gaagaaacgg caagaatgtg gctttcaggg atatcgccta atgctttcag ggcagcattg
      361 acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac tgtcatgcgg
      421 taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acggcgacgc ggtgacggct
      481 cttcatccaa ctcgttaagc atcccttttt gatctggtgg cgcggaaacc tggaactcac
      541 cccaccattt caccagacgc tgcagttcag cgttacgttc aacttcagct cgcaaggcca
      601 acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg cgtttaccct
      661 gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc gtcagacgtt
      721 tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc agcgcgaaag
      781 cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc agcagtgggt
      841 accagaacat ggcggcaaac aagaacgccg ggttcacgcg catatcgtta tggatacgcg
      901 tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg ccattttccg
      961 tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca cacaacagct
     1021 tataggtttc gtaaccgtag cccgcttgta gcagtttaag cgattcttca aacaggcgtg
     1081 ccggtgggat atcgttcagc agggtagcga ggcgagggat cggttctgcg gtttccgggc
     1141 tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt accggatctt
     1201 cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc agatccttca
     1261 tgccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg ctgttgatag
     1321 tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg cgcagcaaca
     1381 tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg tggtgtccac
     1441 ggaaggttgc aacttcgata atctccgggc caaacatcac atgagccaga cggaaacggc
     1501 gacccacaag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg gcgttagtgg
     1561 ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg ccgccaacca
     1621 gccaggcttc gtatcccgct ttattgagcc tgtacattac cttcagggca ttttcactga
     1681 tatctttgcg ggaaatagca tgctgctcac gcgggatcac cgtcacctgt ggacgggcga
     1741 ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa ttagcgactc
     1801 gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa aatagcggct
     1861 aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg actcgggcac
     1921 ggcggtcagc gtccagtttt tgacggctga ctgaaggatt tgctcgacgc tgaaatcctg
     1981 ccagtgtgct tctacctgct gccccagaaa ttgaagtgcc gcgattagta ccgggcgtgg
     2041 atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc cttgtggatt
     2101 aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct ggtacagcga
     2161 gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg taacgccctg
     2221 gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac ggcgatgaat
     2281 gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgccgcgca gctggtcagt
     2341 aaattgcgtg accggatgct gctggcggat acgcactgcg gcgttgtctg gtccatgatg
     2401 caacacccgg caatgaccgt cgtaaatacc gacaatgctt tgaatacgcg cacgcgtaca
     2461 ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac gataggcgtc
     2521 gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat gttccagctg
     2581 gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat cttctatgcg
     2641 taccagccag cgaccttgcc gggcgcgagc ctgcaaatag ctgccgagcg cggcgatcag
     2701 agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat actgtgtgtc
     2761 tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt aaacgtgatg
     2821 gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg ttttgcagtc
     2881 gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt caacaccgca
     2941 ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct cgatcttttt
     3001 gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt cttcctgggc
     3061 tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg taacggtgcg
     3121 atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga agtgcgccag
     3181 ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca ccccagcgat
     3241 ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt tgcttctcct
     3301 taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata aatagcagat
     3361 gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag gaaaagcgta
     3421 tttgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca atgtaaccgg
     3481 cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag ccagaatttc
     3541 tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga tgtggcgcgc
     3601 gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg cacgctggcc
     3661 ttcagccgct acgttcatca actcccgaag gtgtttctga cctcgttcag tgaccgcatc
     3721 gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa tatagcagtc
     3781 tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttctgcgc catattttac
     3841 ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa ggatagcctc
     3901 tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata aatgcgccgc tctggctttg
     3961 agttagttcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg aataccagac
     4021 ggtatcgcca cgcgttgcac aaccggtcat cgcacccgta ttcgggcagt gtagcgttaa
     4081 ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct gaattagcgt
     4141 cgcgcgctgt agaaggggag agaattccat tgcgacaatc cttgttattg cattagcgcc
     4201 cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc gtaaagggtg
     4261 aactccgtca ccgcataaga ccagttaaaa cctggcggcg ggattgtcac cgcctcgctg
     4321 gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa acaaccgctg
     4381 cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg cggctgacgc
     4441 atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag cgtgagtgtg
     4501 aaaccaggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt ctcggcgctc
     4561 acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg acgtcccgcc
     4621 tcaggtggga agtgtgtggc gcgccagtgg ataatctgtt cgcggatttc tgcaggtaag
     4681 tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt atgaattacc
     4741 gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt gtcgtcgttg
     4801 cccgttgctg ccgttttacc tgaattactt accgctctcg attgtgcgcc gcaggtatta
     4861 ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact gctggcgcat
     4921 cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc gcgtaacgtc
     4981 gcgcaacggc tggaggagct gcttaacgaa aagccaggcg ataccgttgg ctaccggatg
     5041 cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tagttaccga aggcgtgctg
     5101 acgcggatga tccagcgtga cccggagttg agcggtgttg gattggtgat ccttgatgaa
     5161 tttcatgagc gtagcttgca ggcggatttg gcgttggcgc tgttactcga tgtgcaacaa
     5221 ggtctgcgtg atgaccttaa actgctaatt atgtcggcta cgctggacaa cgaccgcttg
     5281 cagcaaatgc tgccagaagc gcccgtcgtc atctcagaag ggcgctcgtt tccggttgaa
     5341 cgccgttatt taccgctgcc cgcgcatcag cgttttgacg atgccgttgc ggtagccacc
     5401 gctgaaatgc tgcgtcagga aagcggatca ttactgttat ttttacctgg cgtcggagaa
     5461 attcagcgtg tgcaggaaca actggcttcg cgcatcggca gtgatgtatt gctctgcccg
     5521 ctgtatggcg cgttgtcgct gaacgatcag cgaaaagcga tcctcccggc accgcaaggg
     5581 atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat cgaaggtatt
     5641 cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgtt ttgatccgcg cacggggcta
     5701 acgcgactga ttactcaacg cgttagccag gcatccatga cgcagcgtgc cgggcgcgcc
     5761 gggcgtctgg agccgggtat ctgcctgcat ctaatcgcca aagaacaagc agaacgcgct
     5821 gccgcgcaaa gtgaaccgga aatcttacaa agcgatcttt ccggtttgct gatggaatta
     5881 ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc gccaacggtg
     5941 aatctactgg ccgcgaaacg cctgttacaa atgctggggg cgctggaggg tgaacggctt
     6001 agtgcgcagg ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc ggcaatgctg
     6061 gttagcgcga agaacgacga cgaagctgct accgcggcaa aaattgccgc cattctcgaa
     6121 gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa tcaaccagcc
     6181 tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgtggcgg tgaggcagac
     6241 agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc tcgtcgccgt
     6301 gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga tgccgacgac
     6361 gcgctaagcc gccacgaatg gttgatcgca ccgttattat tgcagggcag cgcctcgccg
     6421 gatgcgcgga ttttactggc gctgccggtc gatattgatg agttagtaca acgctgcccg
     6481 cagctggtac agcagtctga cactgtggag tgggatgacg cgcaaggtac gctgaaagcc
     6541 tggcgtcggc tgcaaatcgg tctgctgacg gtgaaagtgc agccgctggc gaaaccctcg
     6601 gaagacgagt tgcatcaggc gatgcttaat ggcatccgtg ataaaggttt aagcgtgctc
     6661 aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc aaagtggttg
     6721 ccggaatatg actggccagc ggttgatgat gaaagtttat tggcgacgct ggaaacgtgg
     6781 ctgctgccac atatgactgg cgtacattca ctacgcggcc tgaaatcact cgacatttat
     6841 caggcactac gcggattact tgattgggga atgcagcaac gtctggatag tgaattgcct
     6901 gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga agataacccg
     6961 cccgcgctgg cggtgagaat gcaagagatg tttggcgagg ccaccaatcc gacgatcgcc
     7021 caggggcgcg tgccgctggt gctggagttg ctttcacctg cccaaagtcc attacaaatc
     7081 acacgagatt tgagcgcctt ctggaaagga gcgtaccgtg aggtgcaaaa agagatgaaa
     7141 gggcgttatc ccaaacatgt ctggccggac gacccggcaa atactgcacc gacgcgacgg
     7201 acgaaaaagt attcgtaagg ttggttttct ccctctccct gtgggagagg gccggggtga
     7261 gggcatcagc gcgcacgttc accctcaccc taaccctctc cctcaaggga gaggggaccg
     7321 atcgaacaca aattttgaga gatatcttct tctgtcttgt aacagaagaa cagaaaatcg
     7381 ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg cgagccaatt
     7441 ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg tcgttacgaa
     7501 gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat gccgcgcaaa
     7561 ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct atggctactg
     7621 ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta tctcgatcaa
     7681 aaaattcgta gccgtattga tggcaaggtc tggcaactgc ctgcggcagt ttatggccga
     7741 atggtcaatc ttgagccaga tatgaccatc agcaagaacg agatggtgaa gctgctggag
     7801 gcgactcagt atcgtcaggt gtcgaaaatg acccgtcctg gcgaatttac cgtgcaggcc
     7861 aacagcattg agatgatccg ccgtccgttt gatttcccgg acagtaaaga agggcaggtg
     7921 cgtgcgcatc tgacctttga tggtgatcat ctggcgacca tcgtcaatat ggagaacaac
     7981 cgtcagttcg gctttttccg tcttgatccg cgtctgatca ccatgatctc ctcgccaaac
     8041 ggtgagcaac gtctgtttgt gccgcgcagt ggtttccctg atttgctggt ggatactttg
     8101 ctggcgacag aagaccgcca tttctacgag catgatggga tcagcctcta ctcaattggg
     8161 cgtgcggtgc tggcaaacct gaccgcaggt cgcacggtgc agggtgcgag tacgctgacg
     8221 caacagctgg tgaaaaacct gttcctctcc agcgagcgct cttactggcg taaagcgaac
     8281 gaagcctaca tggcgctgat catggacgcg cgttacagca aagaccgtat tcttgagctg
     8341 tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg cttcccgctg
     8401 gcaagcttgt attactttgg tcgcccggta gaagagctaa gcctcgacca gcaggcgctg
     8461 ttagtcggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc gaaactggcg
     8521 ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc aacagattat tgatcaagaa
     8581 ctctatgaca tgttgagtgc ccgtccgctg ggggttcagc cgcgcggtgg ggtgatctct
     8641 cctcagccag cctttatgca actggtgcgt caggagctgc aggcaaaact gggcgataag
     8701 gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc ccaggacgcg
     8761 gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa gttgagcgat
     8821 cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc gatggtggga
     8881 ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg ttcgattggt
     8941 tcccttgcaa aaccagcgac ttatctgacg gctttaagcc agccgaaaat ctatcgcctg
     9001 aacacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcct ggtctggtca
     9061 ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt ggatgcgttg
     9121 acccgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct gcctgcagtt
     9181 acggatacct ggatcaaact gggcgtaccg aaagatcagc tgcatccggt tccggcgatg
     9241 ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcgttcca gaccatcgcc
     9301 agcggtggta accgtgcacc gctttctgca ctgcgttcgg taatcgcgga agatggcaag
     9361 gtgctgtatc agagcttccc gcaggcagaa cgcgctgttc cggcgcaggc ggcgtatctg
     9421 acactatgga ccatgcagca ggtggtacaa cgtggtacgg gtcgtcagct tggggcgaaa
     9481 tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt agatacttgg
     9541 tttgcaggca ttgacggcag cacggtgacc atcacctggg caggccgtga taacaaccag
     9601 ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta tctggctaac
     9661 cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat gggcgtggac
     9721 tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg gaccagcgat
     9781 ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc cgtcaggcaa tccgtttgat
     9841 cagtcttctc agccgcaaca gcaacctgct cagcaagagc agaaagacag cgacggtgta
     9901 gccggttgga tcaaggatat gtttggtagt aattaacatc taagcgtgaa ataccggatg
     9961 gcgagttgcc atccggtaaa ataacatccc atctaagata ttaacccttt cttttcatct
    10021 ggttgtttat taacccttca ggaacgctca gattgcgtac cgcttgcgaa cccgccagcg
    10081 tttcgaatat tatcttatct ttataataat cattctcgtt tacgttatca ttcactttac
    10141 atcagagata taccaatggc gcgttccaaa actgctcagc caaaacactc actgcgtaaa
    10201 atcgcagttg tagtagccac agcggttagc ggcatgtctg tttatgcaca ggcagcggtt
    10261 gaaccgaaag aagacactat caccgttacc gctgcacctg cgccgcaaga aagcgcatgg
    10321 gggcctgctg caactattgc ggcgaagcac tctgctactg cgactaaaac ggatacacca
    10381 attgaaaaaa cgccgcagtc tatttcggtt gtgacaaatg aagagatgca gatgcatcaa
    10441 tttcagtctg taaaagaagc attaggttat acacctggcg ttacggttag cagtcgtggt
    10501 gcttctaata catatgattt tgttatcatt cgtggtttct catctgttgg tctgaatcaa
    10561 aataattacc ttgatggact aaaacttcag ggcaactttt ataacgatgc tgtgattgat
    10621 ccttacatgc tcgagagggt tgaactgatg cgtgggccga cgtctgttct ctatggaaaa
    10681 agtaatcctg gtgggattat ttctatggtg agtaagcgtc cgaccactga acccctgaaa
    10741 gaaattcagt ttaaaatggg gacggataat ctgtttcaga ccggattcga tttcagtgat
    10801 gcactggatg ataacggtga gttctcttac cgtttgacgg gcctcgctcg ctcaacaaac
    10861 gaacagcaga aaaactctga atctcagcgt tataccattg ctccatcatt ctcatggcgt
    10921 ccagacgaca aaaccaattt tactttcctg tcctatttcc agaatgaacc agaaacgggt
    10981 tattacggtt ggttgccgaa agaggggacc gttgagccat tgcctaatgg taagcgtcta
    11041 ccgactgact tcaatgaagg tgcatcgaat aatacatact cccgtaacca gaaaatggtg
    11101 ggatatagtt ttgaacatgg ttttaatgac accttcaccg tgcgtcagaa tctgcgtttc
    11161 agtgaaatga aaacctcaca gaaaagtgtt tatggcacag ggattgctgg tgatggtcat
    11221 accctaaacc gcgggacagt ggtggataat gagcgtctac aaaactttag cgttgatacc
    11281 caacttgaaa gtaagtttgc tacaggtgac gtcgagcata ctttgctgac aggggtagac
    11341 ttcatgcgga tgcgtaatga tatcaatgcc agctttggat ccgcaccatc catcgatctt
    11401 tataacaaat atcaccctga atactttgca tttggtagcg cagagccata ccagatgaat
    11461 gaaagcaaac aaacaggtat ttatgttcag gatcaggcgg aatggaataa atgggtattc
    11521 actctggggg gacgctacga ttggtctaag caagcgacca ctgttcgtga aaactcttat
    11581 acgccgactg aaggttatat tgagcgcaat gatcatcagt tcacctggcg cggtggtgta
    11641 aattacttat tcgataatgg tatttcacct tactttagct atagccagtc ctttgaaccg
    11701 agtgctttcg atctgtggag caacccacgc atttcctata agccatcgaa aggtgaacag
    11761 tatgaagctg gcgtaaaata tgttccgaat gatatgccgg tcgttgttac gggcgcagtc
    11821 tatcaattga cgaaaacaaa taacctgaca gcagacccaa caaacccatt agcgcaagtc
    11881 ccagcaggtg agattcgcgc tcgtggtgtg gaacttgaag caaaagcggc gttaaatgcc
    11941 aatattaact tgacggcttc ttatacctac accgatgcgg aatacaccaa agacaccaat
    12001 ctcaaaggta aaactccaga acaagtaccg gagcatatgg catctctctg gggggattat
    12061 accttcaatg aagggccgct ttctggttta acattgggaa caggtggtcg ttttattggt
    12121 tccagctatg gtgatccggc aaacactttt aaagtgggta gcgcagctgt aatggatgct
    12181 gttgtaaaat atgatctggc acgctttggt atggcgggat ccagccttgc tgtgaacgtc
    12241 aacaatttgc tcgatcgtga gtatgttgcc agttgcttcc agacttatgg ctgcttctgg
    12301 ggcgcagaac gtcaggtcgt tgcaaccgca accttccgtt tctaatttct cttttggggc
    12361 acggatttcc gtgcccattt cacaagttgg ctgttatgca ggaatacacg aatcattccg
    12421 ataccacttt tgcactgcgt aatatctcct ttcgtgtgcc cgggcgcacg cttttgcatc
    12481 cgctgtcgtt aacctttcct gccgggaaag tgaccggtct gattggtcac aacggttcta
    12541 gtaaatccac tctgctcaaa atgcttggcc gtcatcagcc tccgtcagaa ggggagattc
    12601 ttcttgatgc ccagccgctg gaaagctgga gcagcaaagc gtttgcccgc aaagtggctt
    12661 atttgccgca gcagcttcct ccggcagaag ggatgaccgt gcgtgaactg gtggcgattg
    12721 gtcgttaccc gtggcatggc gcgctggggc gctttggggc ggcagatcgc gaaaaagtcg
    12781 aggaagctat ctcgctggtt ggcttaaaac cgctggcgca tcggctggtt gatagtctct
    12841 ctggcggcga acgtcagcgg gcgtggatcg ccatgctggt ggcgcaggat agccgttgtc
    12901 tgttgctcga cgaaccgacc tcggcgctgg atatcgccca ccaggttgat gtgctggcgc
    12961 tggtgcaccg tttaagtcag gagcgtggcc tgacggtcat tgccgtgttg cacgatatca
    13021 atatggcggc acgctactgt gattatctgg tcgccctgcg cggtggtgaa atgattgctc
    13081 agggaacgcc tgcggaaatt atgcgcggcg ataccctcga aatgatttat ggcatcccga
    13141 tgggtatttt gccgcatccg gcgggtgctg cacctgtgag ttttgtttat tgatgagcgg
    13201 cttacctctt atttcgcgcc gtcgactgtt aacggcgatg gcgctttctc cgttgttatg
    13261 gcagatgaat accgcccacg cggcggctat tgatcccaat cgtattgtgg cgctggagtg
    13321 gttgccggtg gaattactgc tggcgctcgg catcgtgcct tacggcgtag cggataccat
    13381 caactatcgc ctgtgggtca gcgaaccacc attgccggac tcagtgatcg acgtcggttt
    13441 gcgcacagaa cctaaccttg aactgctgac cgaaatgaaa ccgtcgttta tggtctggtc
    13501 ggcaggatat ggcccttcag cagaaatgct ggcacgtatt gcgccggggc gtggatttaa
    13561 cttcagcgac ggcaaacagc cgctggcgat ggcgcgtaaa tcactgacgg aaatggcaga
    13621 tttacttaac ctgcaaagcg cagcagaaac gcatttagcg caatatgaag actttatccg
    13681 cagcatgaaa ccccgctttg tgaagcgtgg tgcacgcccg ttattgctga cgacgcttat
    13741 cgatccgcgc catatgttgg tcttcggtcc aaacagcttg ttccaggaaa ttcttgatga
    13801 gtacggcatc ccaaatgcct ggcaagggga aaccaacttc tggggcagta ccgccgtcag
    13861 tatcgatcgt ctggcagcgt ataaagacgt tgatgtgctc tgttttgatc acgacaacag
    13921 caaagacatg gatgcgctaa tggcaacccc gctgtggcag gccatgccgt ttgtccgcgc
    13981 cggacgcttt cagcgcgtac ccgcagtctg gttttatggt gcgacgctct cggcaatgca
    14041 ctttgtgcgc gttctggata acgccatcgg aggtaaagcg tgagtaaacg aattgcgctt
    14101 ttcccggcgt tattgctggc gctgctggtg attgtcgcta cggcgctgac ctggatgaac
    14161 ttttcgcagg cgctgccgcg tagccagtgg gcgcaggccg cctggtcgcc ggatattgac
    14221 gtcatcgagc agatgatttt tcactacagc ttgctgccgc gtctggcgat ttcgctgctg
    14281 gtgggcgcag gcctggggct ggtgggcgtg ctgtttcagc aagtgctgcg taatccgctg
    14341 gcggaaccga cgacacttgg cgttgctaca ggcgcgcaac tggggattac cgtcactacg
    14401 ctctgggcga tcccaggggc gatggcgagt cagtttgctg cgctggcggg cgcttgcgtt
    14461 gttggcttaa tcgtctttgg cgtcgcgtgg gggaaacggc tttcgccggt aacgctgatc
    14521 ctcgcggggc tggtagtgag cctttattgt ggggcaatca atcagttact ggttatcttc
    14581 caccatgacc aactgcaaag catgttcctg tggagcaccg gaacgctgac gcaaaccgac
    14641 tggggcggtg ttgagcgttt atggccgcag ttgctgggcg gtgtgatgct gacgttgctg
    14701 ctacttcgcc cgttaaccct gatggggctt gatgatggcg tggcgcgcaa tctcgggctg
    14761 gccttgtcgc tcgcgcgtct ggcggcgctg tcgctggcga ttgtcatcag tgcgctgctg
    14821 gtgaacgcgg tggggattat cggctttatc gggttgtttg cgccgttgct ggcaaaaatg
    14881 ctgggggcgc ggcgtctgct gccgcgactg atgctggcgt cgttgattgg tgcgctgatc
    14941 ctctggcttt ccgatcaaat catcctctgg ctgactcgcg tgtggatgga agtgtccacc
    15001 ggttcggtca ctgcgttgat cggtgcgccg ctgctactgt ggctgttgcc gcgtttacgc
    15061 agcattagcg cgccggatat gaaggtcaac gatcgtgtcg cggctgaacg ccaacatgtg
    15121 ctggcgtttg ccctcgcggg cggcgtgctg ctgttgatgg ctgtggtggt ggcgctgtcg
    15181 tttggtcgtg atgcgcacgg ctggatgtgg gcgagcgggg cgttgctcga tgatttaatg
    15241 ccctggcgct ggccgcgaat tatggcggcg ctatttgcgg gcgtcatgct ggcggtggcg
    15301 ggctgtatta ttcagcgact gaccggcaac ccgatggcaa gcccggaagt gctggggatt
    15361 agctccggcg cagcgtttgg cgtggtactg atgctgtttc tggtaccggg taatgctttt
    15421 ggctggctgt tacccgcggg aagtatcggt gccgcggtga cgctgttgat tattatgatc
    15481 gccgccgggc gcggtggatt ttccccacac cgcatgttac tggcggggat ggcgttaagt
    15541 accgcattca ccatgctttt aatgatgttg caggcgagtg gtgacccgcg aatggcgcaa
    15601 gtgctcacct ggatctctgg ttcgacctac aacgccaccg atgcgcaggt ctggcgtacc
    15661 ggaattgtga tggtgatttt gctggcgatt accccgctgt accgccgctg gctgaccatt
    15721 ttacctctgg gcggtgatac cgcacgagct gtgggaatgg cgctgacgcc gacgcgaatt
    15781 gcgctgctgc tgttagcggc ttgcctgacg gcgacctcga cgatgactat tggaccgttg
    15841 agttttgttg gtttaatggc accgcatatt gcgcggatga tgggttttcg acggacgatg
    15901 ccacacatcg taatttcggc gctggtgggt ggtttactgc tggtattcgc tgactggtgt
    15961 gggcggatgg tgctgtttcc attccagatc ccggcggggc tgctgtcgac ctttatcggc
    16021 gcgccatatt ttatctattt gttgagaaag cagagccgtt aattgagtgc ctcgcccggt
    16081 gggcgaggca aaattatcac aacttcgcaa atacccgacg tgcagcatcg atggtgttat
    16141 tgatatcttc catgctgtgc gccacagaca taaagcccgc ttcaaacgct gacggtgcca
    16201 ggtaaacacc ttcgtccagc atcatatgga agaaacgctt aaagcgttcc acgtcacagg
    16261 ccatcacatc ctgatagcac gtcacggact cggcgtcggt aaagaaaata ccgaacatgc
    16321 cgccaacgtg gttaacgacc agcggagttc cggcttcttc tgccgcttcc agcagacctt
    16381 ctgccagacg tgttgtcagc tcatccagcg tttcgtgaac gcccggctgc gcgacttcat
    16441 tcagacaggc gaaacccgct gccatcgcaa tcgggttacc ggaaagcgta cccgcctgat
    16501 agaccggacc cgtcggggcc agcgcatcca ttacatcacg acgaccaccg aatgcgccta
    16561 ccggcattcc accgccgatg attttgccga ggcaggttaa atctggcact acgccgtaat
    16621 aatcctgtgc gccagccagt gccacgcgga agccggtcat cacttcatcg atgatcagca
    16681 acgcgccaaa ttcgtcgcac agcgcacgca gacctggcag gaactctggc tgcggcggaa
    16741 cacagttcat attgcctgcc accggctcga cgataataca ggcaatctct tgcgggtatt
    16801 gctcaaacgc ggcgcgtaca gaagccagat cgttataagt acaggttaag gtatgtttgg
    16861 cgaaatctgc cggaacaccc ggcgagtttg gctggcctaa cgtgagtgcg ccagaaccgg
    16921 ctttcaccag caggcagtca gcgtgaccgt ggtaacaacc ttcaaattta atgattttgt
    16981 cgcgaccggt aaaaccacgg gccaggcgga tggcgctcat ggtcgcttca gtgccggagt
    17041 tcaccatgcg caccatatcc atggtcggga ccagttcggt caccagttgc gccattttca
    17101 cttccatttc ggtcggtgca ccaaagctta aaccacgctc ggcggcttca atcacggcat
    17161 tgcggatagc cggatggtta tggcccagca ccatcggccc ccaggaaccg acataatcga
    17221 tataggcttt gccatcaaca tcgtacagat aagcgccgtc cgctttttcg ataaacagtg
    17281 gagtgccgcc cacgccagta aaggcgcgaa caggggagtt cacaccgcca gggagcagct
    17341 cgcgcgctgc gctgtaaaga ttttcagact tactcatgga gggttcctga ttcgtagaaa
    17401 aagtgaatgg ctgctattct atgttattca taacaagtta aatactcgtc aaacatcagg
    17461 ctgcttgtac tggtgcaatc ctgatttctt agagtataaa agttttgtgc atttgaaaca
    17521 ttacgctttg caaaggattt tcatggaacg tgcgagtaaa atgccgtcat cttatttgta
    17581 tgaccaataa gtgatcattg gatgaaaact gatactccct ctttagaaac accgcaggcc
    17641 gcgcgcctgc gacgcagaca actgattcgc caacttcttg agcgcgataa aaccccgtta
    17701 gccattttgt ttatggcggc agtcgtcggc acgcttgtcg ggctggcagc ggttgctttt
    17761 gacaaaggtg tcgcctggtt gcagaaccaa cgtatggggg cgctggtaca tactgctgat
    17821 aattatccgc ttctgttaac cgtcgctttt ctctgttcgg cggtgctggc gatgttcggc
    17881 tactttttgg tgcgcaaata cgcgccggaa gcaggtggtt cggggatccc ggaaattgaa
    17941 ggggcgctgg aagatcaacg tcccgttcgc tggtggcgtg tgttgccggt gaaattcttt
    18001 ggcgggctgg ggacactcgg cggaggcatg gtgctggggc gcgaagggcc aaccgtgcag
    18061 atcggcggca atatcggtcg catggtactc gatattttcc gcctgaaagg tgatgaagct
    18121 cgccatacgc tgctggcaac cggtgctgct gcggggctgg ctgcggcctt taacgcgccg
    18181 ctggcgggta ttttgtttat tatcgaagag atgcgtccgc agtttcgcta tacgttaatt
    18241 tcgattaaag cggtatttat tggcgtcatt atgtcgacca ttatgtaccg gatttttaat
    18301 catgaagttg cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg
    18361 ctttatctga tcctcggtat tatttttggc attttcggcc ctatttttaa taaatgggtg
    18421 ctggggatgc aggatttgct gcaccgtgtg cacggcggca atattaccaa atgggtgcta
    18481 atgggcggtg cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc agcaacgtcg
    18541 ggcggcggtt ttaacctgat tcctatcgct accgcaggga atttcagcat gggaatgctg
    18601 gtgtttatct tcgtcgcgcg ggtcattacc accttactgt gcttctcttc cggcgcgccg
    18661 ggcggtattt ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg
    18721 gttgtcgttg agctgtttcc gcaatatcac cttgaggcgg ggacttttgc tattgccgga
    18781 atgggggcgt tactggcggc atctattcgc gcgccgttaa cggggatcat tttggttctg
    18841 gagatgaccg ataactacca gctcattttg ccaatgatta ttaccggtct tggcgcaaca
    18901 ctattagcgc aatttaccgg cgggaaaccg ctatattcgg agattcttgc gcgcacgctg
    18961 gcaaaacagg aagctgagca actggcgcga agcaaggccg catcagccag cgagaatact
    19021 tgaacgaaat accagggtat tagataatgg cgattattat tgggttagaa tttgcccaat
    19081 tgccgatgtc gtttggagca aaatatgagt gatgacgtag cactgccgct ggagtttacc
    19141 gacgcagcag ccaacaaagt taaaagcctg atcgctgacg aagataaccc gaatctgaaa
    19201 ttacgcgtgt atatcaccgg tggcggttgc agcggcttcc agtatggttt cacctttgat
    19261 gatcaggtga acgaaggcga tatgaccatc gaaaaacagg gcgttggcct ggtggttgat
    19321 ccgatgagcc tgcaatatct ggtcggtggt tccgttgact ataccgaagg actggaaggt
    19381 tctcgtttca tcgtgaccaa cccgaacgcg aaaagcacct gcggttgcgg ttcttccttt
    19441 agcatctaat ctgttgttca tgtgccggat aaactatccg gcacatcttc tctcttcaac
    19501 gcccattctc atccagcgca aacgtcggta gcttaagatg ccaacgaata gccgccagcc
    19561 gaatcaatag cgtcacgacc atgcccatca tactggctgt ttccagtggt acggaaaatg
    19621 tgtaataagc cgtagcgtgg acaataccgc cgataataca ggcagttgcg tagatttctg
    19681 tacgtaaaat catggggatt tcgcgggcca gtacatcacg aatgatcccg ccgccaacgc
    19741 cagtaatgac gcccatacaa accgcgatta acggaccggc ttccgcatta aaggctttat
    19801 tcacgccaat gccgacaaac accgccagac caacggcgtc cagcaccggc aacatccatt
    19861 ttggtaagcg tcttggctgg cgcaccagca cgatggtcag catgctggtt accattgcaa
    19921 cgaccagatc ggtgggatct ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc
    19981 gaattgtccc gccgcctaca gcggtaacca cgcccagtac cagaacacca aaagggtcca
    20041 tacgcaattt tccggctaac aaaacgccgg agatggcaaa taccgctgtg ccgactatat
    20101 ccagccaata gacgagcatt gttcaatccc cactgagcac ctaaaaaggt caggcgctaa
    20161 tctacctgtg aaagcgcatt acagagctgt tgtgcagcga ggataatacg tgggcttgca
    20221 cgtcaaacca gtcactcgtg agaggaataa cgggaatttt gagctgttca ccccagtatt
    20281 gtttgatttt aggaatttgg tccggtccgc ctgtaatgac aatcgcctgt ggcgagcgtg
    20341 ctaacacctg ttcgcggcta acttgcggcc aaggaacccg gctgtcttta aagatgtttt
    20401 ctccgccaca aacttcgagt acctggtttt gaatcgactc ttttccactg gtaaatggcg
    20461 gattaatgcc gaattgcaga aaaacacgtt ttttaggttt atcagcatat tgcgctttca
    20521 attgcgcgta ctgatccagc agggattgcg cggcttgttc ggccttatcc ggttgcggac
    20581 tccagggggc cagttgacgt aacgcattgg caatttgttc aatgcttgtc gcatcgaccc
    20641 acatcacttt tattcccggc gaagccagct ggtcaacctg ccgctcggca ttacctccac
    20701 gccaggcaat caccagatcg ggtttcagcg cgacaatgcg ttccagattc atcccctgcc
    20761 aggtggaaac ctgctcaatc ttttgcgctt gtggaggata gtcggaatag ctgctgaccc
    20821 caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga
    20881 cgcgcggtgc cgcgttgagc cacagtggcg cgagaaaaga cagggcgacc agcgccctga
    20941 acagtgactt agccatgtgc cagtttctgc accagtgact caaccatcag gctggactgt
    21001 ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag actgttgatc ggccacgtcg
    21061 gagatggcgc gtacgacaac aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc
    21121 gtcgcttcca tctctacagc aatggcctgt gggaagttgt ggcggatttt cgccagacca
    21181 acagaaccgt tgatgaaagc gtcgccgcta acaatcaggc cacgtacagc gttaagattc
    21241 agttcggcaa tgcaggcctc agcggcagcg atcagtttat cgtcagcttc aaagcctgcc
    21301 ggacagcctg gtaactgacc gtattcataa ccaaatgccg tgacatccgc gtcgtgataa
    21361 cgtgcttcgt ccgagacaac gatatcgccc actttcaacg ttggtgccag gccaccggca
    21421 gaaccggtgt taataatcac atctggcttg cagtgttcca acagcaaagt ggcacccagc
    21481 gccgcagcga ctttaccgat gcccgatttc agaagcgcaa cctcggttcc attcagttgg
    21541 ccggtataga tttcgcaacc gccgagactg atagtttgac gattttcgat tttgtcacgc
    21601 agcagcgtaa cttcttcttc cattgcacca atgatgccga ttttcataga tttactcgcg
    21661 ataagcccga tttgaaggca tagtttacca tgcgcttacg gggaagcgta tttctcacgc
    21721 gggagaggac atggcacaga ttgatttccg aaaaaaaata aactggcatc gtcgttaccg
    21781 ttcaccgcag ggcgttaaaa ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg
    21841 gcgtatcatc aactctccgg caattcgtcg tctgcaacaa aagacccagg tttttccact
    21901 ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg atggaagtcc agcaggtggg
    21961 gcgctacatc gccaaagaaa ttctaagccg tatgaaagag cttaaattac tggaagcata
    22021 cggcctggat gaactgaccg gcccctttga aagcattgtt gagatgtcat gcctgatgca
    22081 cgatatcggc aatccgccgt ttggtcattt tggcgaagcg gcgataaatg attggtttcg
    22141 ccagcgtttg cacccggaag atgccgaaag ccagcctctg actgacgatc gctgcagcgt
    22201 ggcggcacta cgtttacggg acggggaaga accgcttaac gagctgcggc gcaagattcg
    22261 tcaggactta tgtcattttg aggggaatgc acaaggcatt cgtctggtgc atacattgat
    22321 gaggatgaat ctcacctggg cacaggttgg cggtatttta aaatataccc gtccggcgtg
    22381 gtggcgtggc gaaacgcctg agacacatca ctatttaatg aaaaagccgg gttattatct
    22441 ttctgaagaa gcctatattg cccggttgcg taaagaactt aatttggcgc tttacagtcg
    22501 ttttccatta acgtggatta tggaagcagc cgacgacatc tcctattgtg tggcagacct
    22561 tgaagatgcg gtagagaaaa gaatatttac cgttgagcag ctttatcatc atttgcacga
    22621 agcgtggggc cagcatgaga aaggttcgct cttttcgctg gtggttgaaa atgcctggga
    22681 aaaatcacgc tcaaatagtt taagccgcag tacggaagat cagtttttta tgtatttacg
    22741 ggtaaacacc ctaaataaac tggtacccta tgcggcacaa cgatttattg ataatctgcc
    22801 tgcgattttc gccggaacgt ttaatcatgc attattggaa gatgccagcg aatgcagcga
    22861 tcttcttaag ctatataaaa atgtcgctgt aaaacatgtg tttagccatc cagatgtcga
    22921 gcagcttgaa ttgcagggct atcgggtcat tagcggatta ttagagattt atcgtccttt
    22981 attaagcctg tcgttatccg actttactga actggtagaa aaagaacggg tgaaacgttt
    23041 ccctattgaa tcgcgcttat tccacaaact ctcgacgcgc catcggctgg cctatgtcga
    23101 ggctgtcagt aaattaccgt cagattctcc tgagtttccg ctatgggaat attattaccg
    23161 ttgccgcctg ctgcaggatt atatcagcgg tatgaccgac ctctatgcgt gggatgaata
    23221 ccgacgtctg atggccgtag aacaataacc aggcttttgt aaagacgaac aataaatttt
    23281 taccttttgc agaaacttta gttcggaact tcgcgctata aaacgaatct gaagaacaca
    23341 gcaattttgc gttatctgtt aatcgagact gaaatacatg aaaaaaacca cattagcact
    23401 gagtgcactg gctctgagtt taggtttggc gttatctccg ctctctgcaa cggcggctga
    23461 gacttcttca gcaacgacag cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt
    23521 gatgccttca gtggtcagca ttaacgtaga aggtagcaca accgttaata cgccgcgtat
    23581 gccgcgtaat ttccagcagt tcttcggtga tgattctccg ttctgccagg aaggttctcc
    23641 gttccagagc tctccgttct gccagggtgg ccagggcggt aatggtggcg gccagcaaca
    23701 gaaattcatg gcgctgggtt ccggcgtcat cattgatgcc gataaaggct atgtcgtcac
    23761 caacaaccac gttgttgata acgcgacggt gattaaagtc cagctgagcg atggccgtaa
    23821 gttcgacgcg aagatggtcg gcaaagatcc gcgctctgat atcgcgctga tccaaatcca
    23881 gaacccgaaa aacctgaccg caattaagat ggcggattct gatgcactgc gcgtgggtga
    23941 ttacaccgta gcgattggta acccgtttgg tctgggcgag acggtaactt ccgggattgt
    24001 ctctgcgctg gggcgtagcg gcctgaatgc tgaaaactac gaaaacttca tccagaccga
    24061 tgcagcgatc aaccgtggta actccggtgg tgcgctggtt aacctgaacg gcgaactgat
    24121 cggtatcaac accgcgatcc tcgcaccgga cggcggcaac atcggtatcg gttttgcaat
    24181 cccgagcaac atggtgaaaa acctgacctc gcagatggtg gaatacggcc aggtgaaacg
    24241 cggtgagctg ggtattatgg ggactgagct gaactccgaa ctggcgaaag cgatgaaagt
    24301 tgacgcccag cgcggtgctt tcgtaagcca ggttctgcct aattcctccg ctgcaaaagc
    24361 gggcattaaa gcgggtgatg tgatcacctc actgaacggt aagccgatca gcagctttgc
    24421 cgcactgcgt gctcaggtgg gtactatgcc ggtgggcagc aaactgaccc tgggcttact
    24481 gcgcgacggt aagcaggtta acgtgaacct ggaactgcag cagagcagcc agaatcaggt
    24541 tgattccagc tccatcttca acggcattga aggcgctgag atgagcaaca aaggcaaaga
    24601 tcagggcgtg gtagtgaaca acgtgaaaac gggcactccg gctgcgcaga tcggcctgaa
    24661 gaaaggtgat gtgattattg gcgcgaacca gcaggcagtg aaaaacatcg ctgaactgcg
    24721 taaagttctc gacagcaaac cgtctgtgct ggcactgaac attcagcgcg gcgacagcac
    24781 catctacctg ttaatgcagt aatctcactc aaccccttcc tgaaaacggg aaggggttct
    24841 ccttacaatc tgtgaacttc accacaactc catacatctt catcatcctt taggcatttg
    24901 cacaatgccg tacgttacgt acttccttat gctaagccgt gcataacgga ggacttatgg
    24961 ctggctggca tcttgatacc aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca
    25021 tcgataccaa tatcaacgta atggatgccc gtgggcgaat tatcggcagc ggcgatcgtg
    25081 agcgtattgg tgaattgcac gaaggtgcat tgctggtact ttcacaggga cgagtcgtcg
    25141 atatcgatga cgcggtggca cgtcatctgc acggtgtgcg gcaagggatt aatctaccgt
    25201 tacggctgga aggtgaaatt gtcggcgtaa ttggcctgac aggtgaacca gagaatctgc
    25261 gtaaatatgg cgaactggtc tgcatgacgg ctgaaatgat gctggaacag tcgcggttga
    25321 tgcacttgtt ggcgcaggat agccgtttgc gggaggaact ggtgatgaac ctgattcagg
    25381 cagaggagaa tactcccgca cttactgaat gggcgcaacg gctggggatc gatctcaatc
    25441 aaccgcgagt ggtggctatt gttgaggtcg acagcggtca gcttggtgtg gacagcgcaa
    25501 tggcggagtt acaacaactg caaaacgcgc tgactacgcc cgagcgtaat aatctggtgg
    25561 cgattgtctc gctaaccgaa atggtggtgt tgaaaccggc gttgaactct tttgggcgct
    25621 gggatgcaga agatcatcgt aagcgagttg aacaactgat tacccgcatg aaagagtacg
    25681 gccggctgcg ttttcgcgtt tcactgggca actattttac cggtcctggc agtattgccc
    25741 gatcctatcg tacggcgaaa acgacgatgg tggtgggtaa acagcggatg ccagaaagtc
    25801 gctgctattt ttatcaggat ctgatgttac ctgtgttact cgacagtttg cgtggcgact
    25861 ggcaggccaa cgaactggcg cgaccgctgg cgcggctgaa agcgatggac aataacggct
    25921 tgctgcgacg aacgctggcg gcgtggtttc gtcacaatgt gcaaccgctg gcaacgtcaa
    25981 aggcgttgtt tatttatcgt aataccctgg agtatcggct taatcgtata tcggaactga
    26041 ccgggcttga tttgggcaat tttgatgaca ggttgctgct gtatgtggcg ttacaactgg
    26101 atgaagagcg gtaggttatg cgttaaggtt gaggcggcgc tgtctcattc ccctcagccc
    26161 gaaggggaga ggggaatgcc cgaattaacc gtcaattcgg gattatttac gcgttagttt
    26221 ttccagatcg gcttcaatct cgctgatctt attggttacg actgactcca ggtgacgtaa
    26281 gtcgtcgagg atcttacgct taagatcaac ttcgctgcgg tcacgctggc agatttgatc
    26341 aagctcatca atgatatacc gtagattcgg gctgatttcc tggacttctt tataaccctg
    26401 acccacacca tcagcgacga ccgttttacg ctgacgcgga tatttaaact taacgctctt
    26461 ggcgaaaaac tcgcctttgt ctttctggaa atagattttc agaatatcgt tgttggcttc
    26521 ctgccggagg ctgtaacgat caatttcttc aggattggta atacccagac ttttcagatt
    26581 gtcgtacata gcgttacctc aaaatgagtc agtaaattgt gcttatttta gcatttggcc
    26641 tagcccgccc ggctcgactg tgatcgcaga aagccttttt cttccagact tctatcatgg
    26701 cgtaaaaatc aaaaaattac ctgctttatt gtggtgataa aattcacgtt ctacaccagt
    26761 tccagcatcg ttacttccgg gcggcagttc agacgcaaac catacaaact gcccacgcca
    26821 cgggttgtgt agatgtgtcg ttcgccaaag gcatttaatc cggcgacgta acgtttatct
    26881 tcgacagggg caaaaggttc accaaccaac ggtacgcgca gttgcccgcc gtgagtatgg
    26941 ccgcacagca tcagatccca gggttcatca cgcatgactt ctttgctgtc gggattatgt
    27001 gccagcatca gtcttggcag atttgcttcg ctggcgggag gtggtttgca ttgtccggcc
    27061 cataagtcac cggtgcctac cagttcgaat tgcctgttcg gcgtggcgat caccgtagcc
    27121 tggttaaaca acaccgtgat gcccactgat ttcaacgtct cgccaattaa gtgatttttt
    27181 tctgtaccaa cagggcgatc gtggttgccg aagcaggcaa acgtcggcgc acattcggca
    27241 aggggggaga ggacatcact aaacgccgaa aaattcagcg gcatatcaaa taatacgtaa
    27301 tcgccgccca gtaatatcaa atcgggcttt tgttctatgc caagagcaat cgcgtcagaa
    27361 atcaggctta aaggaacaaa acgagagtaa tggagatcgg ccagaaaaag aattttgaat
    27421 ggtgctgcgt tgtctttaaa aaaggcgagg cggtgacgga ttaattcaaa ccagcctggc
    27481 tcccagtaat gcatataacc gaaacctgag ctcgtggcta tcgtcgcggc agtagcctgc
    27541 aacaatcggc ggcgtgaaat catcgctcat ccctgcaaaa aaaatcgggc agcgtcgtgc
    27601 tgcccgtgtg catactttta gtcgatggta cgcagcagtt cgttaatgcc gactttgccg
    27661 cgagttttcg cgtcaacttt cttaacgata accgcacagt agaggctgta tttgccatct
    27721 tttgacggca gattacctga aacaaccaca gaccccgccg gaacgtgacc gtagtggatt
    27781 tcgccggttt cacggtcgta aatacgggtg ctctgaccaa tgtatacgcc catggaaatg
    27841 acggaacctt cttcgacaat caccccttca actacttcag agcgtgcgcc aatgaagcag
    27901 ttatcttcaa tgatggttgg gttagcctgc agcggttcca gcacgccgcc gatgcccacg
    27961 ccaccggaaa ggtggacgtt tttaccaatc tgcgcacaag aaccgacggt cgcccaggta
    28021 tcaaccatgg tgccttcatc aacatatgcg ccgatgttga cgtaagacgg catcagcacg
    28081 gtgttacggg caataaacgc accctgacgt accgccgctg gtggcacaac gcggaagcct
    28141 tctttctgga aacgtgcttc gtcgtagtcg gcgaatttca ttggcacttt gtcgaagtag
    28201 cggctttctg ccccttcgat cacctgatta tcattaatac ggaaagagag cagcaccgct
    28261 tttttcaacc actgatgcgt cacccactga ccgtcaattt tttccgctac acgcagtgca
    28321 ccggaatcca gcagggcgat cacctgattt accgcttcgc gggtaacggt gtctgcgttg
    28381 gttggcgtga tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg taactgctgc
    28441 attgttaaac tcttttcata tcagtaaaca catcaccctt tatcgtttgg attgagggcc
    28501 tctgtcaacc gctgatgcac ttcctgctgc aactcgttat taagcgcacg ccggtcagcg
    28561 gtggcaatta tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg ggcaccatga
    28621 agcgaaattc ccagatcggc aaaaattttc ccgactctcg ccagcagtcc tggctggtcg
    28681 agcgcgataa gctcaaggaa cgatttgcgg tcggtatggg tcggcaaaaa cgttacttcg
    28741 gtttcaacag taaaatggcg taatttggcg ggttggcgac ggggctgcgg tggctgccag
    28801 ctactttgcg tcagtacttg ctccagacca aaccgaataa cctcatgacg atctgcggac
    28861 agcgggctgc catcgggttc cagcacgata aaggtatcca tcgccatacc gtcgcgagtg
    28921 gtgaaaattt gtgcgtcgtg aacacttaaa ttgcggcggt ctaattcggc acagacggcg
    28981 gcaaacagat aggggcggtc cgggctccag ataaaaatct cggtgcctcc acgcgtagcc
    29041 tgcgggctaa gcaataccag tggtttgctt aaatcatgct gtaataaatg gcgggcatgc
    29101 caggccagtt gatttgggct atggcggaca aaatagttag cacgacagcg tgaccaaatt
    29161 tggtgcagcg cctcttcgtc gatgttatcc atgcgcagta gtgccagtgc ctggagttgg
    29221 tgatggcgaa cccgttcgcg catatccggc gtgttttgca ttccgcgtcg tagctgcttt
    29281 tcggtggcaa aatagagttc gcgcaacaga ctttgcttcc agctattcca cagcgtttcg
    29341 ttggtggcgc aaatgtcagc cactgtcagg cataccagat agcgcagacg attttccgtt
    29401 tgaacttctt cggcaaactg cttgatgact tcagggtcct gaatatcgcg gcgttgtgct
    29461 gtcaccgaca tcagcaagtg ctggcgaacc agccaggcaa ccagctgtgt ttcgcgtgag
    29521 ttcagcccgt ggagttctgc aaaatgcact acatcctgag caccgagaat ggagtggtcg
    29581 ccgccgcgtc ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag ctcagttgac
    29641 ggcaggcgcg gccagacgtc cacacacaac ggatggcgct ggcgcgtttc ttcactggca
    29701 aaactctcca gtttcagcat cacgcggata gtatgttcat ccaccgtgta ggcgtggaac
    29761 agatcaaact gcatctgccc gacgatatgc gaccattgcg gcatatacgc gccgagcacg
    29821 ctatggcgat gcattggcaa tagcccgcgc cgcaccgctc cggggtgacg cagaatgctc
    29881 aaaaacagtt ttcgtgcttc cggaatatta cacagcggtt gttgcagatg gcgacgggca
    29941 tggcgtaact ggcgcagcgt ggtggagtaa atgccggtga tcgcactgtt gcgcaccatg
    30001 gtgtagaaca tacgcaagat ggcttccggc tggcgcataa atagtgtttc atcacgcagg
    30061 tcgattagcg taccgcgtag ctgaaactca tcgtcgattg gacgtggttt ttcgtcggca
    30121 ggaagggcga ggatggcttc atcgaacagt tgcagcagca tctggttgag ttcactgacg
    30181 cggcgtgtaa cgcggaagta atccttcatc atccgctcga ccggttcgtt gccttcacca
    30241 ctgtaattca gacgctgagc gacgctaagt tggcgatcga ataacaggcg attatcgtaa
    30301 cggctgacga ccagatgcag ggcaaagcga atacgccaca gtatatgcag acattcgttt
    30361 aattccgccc gctccgctga ggttaagaag ccaaacccaa ccatttcatc cagcgatgtt
    30421 gcgccaaaat gacggcgggc cacccattgc agagtgtgga tatcgcgcaa gccgccaggg
    30481 ctacttttga tgtctggttc aagattgtag ctggtgccgt ggtaacgctg atggcgctgg
    30541 ttctgttctt caactttcgc cgcgtagaac ttgtcggaag gccagaatcc ttcgctaaaa
    30601 atatgttttt gcagttcgag gaagagcgca acatcgccaa ttaataagcg ggattcgatt
    30661 aaattggtgg cgacggttaa atccgataac ccttccagca tgcactcttc aagcgtgcgc
    30721 acactatgac cgacttccag ctttacatcc cagagcagcg ttaacagctc gcccactttt
    30781 tgcgcctgat cgtccgggag ctttttacgg cttaaaatca gtaaatcgat gtcagaaagt
    30841 ggatgcagct cgccacgacc gtagccaccg acggcgacca atgccaggtc ggcaatctgg
    30901 ctgaatcccg cttcaatcca taatcgttgc aggagctggt cgataaactc ggtgcgcgcc
    30961 tcaatcaact gttctgcaga gatcccattg tcaaaggcat cacccagcca acgctggaaa
    31021 gtatcgatat gggcttttat cccaccgacg gttaattcat cacggggcca gacgcatgga
    31081 ttttgcggtt gaccgggcag ggtggggaga gcggtgtttg cgtactgttc tggaagggta
    31141 ttcattgtgc gccactcata agattaaatt atcgcattaa aaaagccggc attcgccggc
    31201 ttcatcttat tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc gtagcgtcag
    31261 aatttcgcag ccgttatcag tcaccacaat agtatgctca tattgtgcag acaagctgcg
    31321 atctttggtt tttaccgtcc agccatcttt catggtgcgg atctcttttt tacccgcgtt
    31381 gaccattggc tcgatggtga acgtcatccc aggtttcagt acgacgttgg tttcacggga
    31441 gtcatagtgc agcacctgcg gttcttcatg gaagccgcga ccaataccgt gtccgcaata
    31501 ttcacgaacg acggagaagc cttctgcttc gacaaacttc tgaatcgccg caccgatttc
    31561 gcgcaggtta atgcctggtt tgaccatgcg cagcgccagg tacaggcttt cttgcgtgat
    31621 gcggcacaga cgttcgccca tgatggtcgg cttaccgacg ataaacattt tcgaggtatc
    31681 gccgtggaaa ccatctttga ttacggtgac atcaatgtta acgatatcgc catctttcag
    31741 cagcttcgca tcgtccggga taccgtggca caccacttca ttaatagaga tgcaaacgga
    31801 tttcggatag ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat taacaatgta
    31861 atcattacag atgcgatcca gctcgccggt gctgacgccc ggtttaacat acggttcgat
    31921 catctccagc acttcggcag ccagtcggcc agcgacgcgc attttttcga tatcttctgg
    31981 ggtcttgatt gagatagcca ttaattctgt ccatcagcgt cggtgatacc gacaatatat
    32041 atgtaagtgc cgtcaatggt atcacacccg ggcaaattga gaatcattct gaatttcgcc
    32101 aaacgtgcca ctgaacgttt tctataatag aaaattcaac gtctgatgct gtacacagcg
    32161 ccaacaatta ttggtgtcca tgacgtattt gtggtataaa gcgcgccgga cttccgatcc
    32221 atttcgtata cacagactgg acggaagcga caatctcact ttgtgtaaca acacacacgt
    32281 atcggcacat attccggggt gccctttggg gtcggtaata tgggatacgt ggaggcataa
    32341 ccccaacttt tatatagagg ttttaatcat ggcaactgtt tccatgcgcg acatgctcaa
    32401 ggctggtgtt cacttcggtc accagacccg ttactggaac ccgaaaatga agccgttcat
    32461 cttcggtgcg cgtaacaaag ttcacatcat caaccttgag aaaactgtac cgatgttcaa
    32521 cgaagctctg gctgaactga acaagattgc ttctcgcaaa ggtaaaatcc ttttcgttgg
    32581 tactaaacgc gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg accagttctt
    32641 cgtgaaccat cgctggctgg gcggtatgct gactaactgg aaaaccgttc gtcagtccat
    32701 caaacgtctg aaagacctgg aaactcagtc tcaggacggt actttcgaaa agctgaccaa
    32761 gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc tgggcggtat
    32821 caaagacatg ggcggtctgc cggacgctct gtttgtaatc gatgctgacc acgaacacat
    32881 tgctatcaaa gaagcaaaca acctgggtat tccggtattt gctatcgttg ataccaactc
    32941 tgatccggac ggtgttgact tcgttatccc gggtaacgac gacgcaatcc gtgctgtgac
    33001 cctgtacctg ggcgctgtag ctgcaaccgt acgtgaaggc cgttctcagg atctggcttc
    33061 ccaggcggaa gaaagcttcg tagaagctga gtaataaggc ttgataactc ccccaaaata
    33121 gttcgagttg cagaaaggcg gcaagctcga gaattcccgg gagcttacat cagtaagtga
    33181 ccgggatgag cgagcgaaga taacgcatct gcggcgcgaa atatgaaggg ggagagccct
    33241 tatagaccag gtagtacacg tttggttagg gggcctgcat atggccccct ttttcacttt
    33301 tatatctgtg cggtttaatg ccgggcagat cacatctccg aggattttag aatggctgaa
    33361 attaccgcat ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat gatggattgc
    33421 aaaaaagcac tgactgaagc taacggcgac atcgagctgg caatcgaaaa catgcgtaag
    33481 tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg cgtgatcaaa
    33541 accaaaatcg acggcaacta cggcatcatt ctggaagtta actgccagac tgacttcgtt
    33601 gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc tggacgcagc tgttgctggc
    33661 aaaatcactg acgttgaagt tctgaaagca cagttcgaag aagaacgtgt tgcgctggta
    33721 gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg cgctggaagg cgacgttctg
    33781 ggttcttatc agcacggtgc gcgtatcggc gttctggttg ctgctaaagg cgctgacgaa
    33841 gagctggtta aacacatcgc tatgcacgtt gctgcaagca agccagaatt catcaaacca
    33901 gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg tacagctgga tatagcgatg
    33961 cagtctggta agccgaaaga aatcgcagag aaaatggttg aaggccgcat gaagaaattc
    34021 accggcgaag tttctctgac cggtcagccg ttcgttatgg aaccaagcaa aactgttggt
    34081 cagctgctga aagagcataa cgctgaagtg actggcttca tccgcttcga agtgggtgaa
    34141 ggcatcgaga aagttgagac tgactttgca gcagaagttg ctgcgatgtc caagcagtct
    34201 taattatcaa aaaggagccg cctgagggcg gcttcttttt gtgcccatct tgtaaattca
    34261 gctaaccctt gtggggctgc gctgaaaagc gacgtacaat gtcgctagta ttaattcatt
    34321 tcaatcgttg acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc gtctataaac
    34381 gcattctgct taagttgagt ggcgaagctc tgcagggcac tgaaggcttc ggtattgatg
    34441 caagcatact ggatcgtatg gctcaggaaa tcaaagaact ggttgaactg ggtattcagg
    34501 ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc tggtctggcg aaagcgggta
    34561 tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac cgtaatgaac ggcctggcaa
    34621 tgcgtgatgc actgcaccgc gcctatgtga acgctcgtct gatgtccgct attccattga
    34681 atggcgtgtg cgacagctac agctgggcag aagctatcag cctgttgcgc aacaaccgtg
    34741 tggtgatcct ctccgccggt acaggtaacc cgttctttac caccgactca gcagcttgcc
    34801 tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt gacggcgtgt
    34861 ttaccgctga tccggcgaaa gatccaaccg caaccatgta cgagcaactg acttacagcg
    34921 aagtgctgga aaaagagctg aaagtcatgg acctggcggc cttcacgctg gctcgtgacc
    34981 ataaattacc gattcgtgtt ttcaatatga acaaaccggg tgcgctgcgc cgtgtggtaa
    35041 tgggtgaaaa agaagggact ttaatcacgg aataattccc gtgatggata aataagggta
    35101 agattccgcg taagtatcgc gggggcgtaa gtctggttat aaggcgttat tgttgcaggc
    35161 agtttggtca cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga ggatggcgag
    35221 cactgctcgg ggccaaaatg gcaaataaaa tagcctaata atccagacga ttacccgtaa
    35281 tatgtttaat cagggctata cttagcacac ttccactgtg tgtgactgtc tggtctgact
    35341 gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat gctgaagtac
    35401 gcatggacaa atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc acgggtcgtg
    35461 cttctcccag cctgctggat ggcattgtcg tagaatatta cggcacgccg acgccgctgc
    35521 gtcagctggc aagcgtaacg gtagaagatt cccgtactct gaaaatcaac gtgtttgatc
    35581 gttcaatgtc tccggccgtt gaaaaagcga ttatggcgtc cgatcttggc ctgaacccga
    35641 actctgcggg tagcgacatc cgtgttccgc tgccgccgct gacggaagaa cgtcgtaaag
    35701 atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta cgtaacgtgc
    35761 gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc agcgaagacg
    35821 acgatcgccg ttctcaggac gatgtacaga aactgactga tgctgcaatc aagaaaattg
    35881 aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt gaacgacaaa
    35941 aacgccgctc agtagatcct tgcggatcgg ctggcggcgt tttgcttttt attctgtctc
    36001 aactctggat gtttcatgaa gcaactcacc attctgggct cgaccggctc gattggttgc
    36061 agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc gctggtggca
    36121 ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg ctatgccgta
    36181 atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca gggtagccgc
    36241 accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga ggatgttgat
    36301 caggtgatgg cagccattgt tggcgctgct gggctgttac ctacgcttgc tgcgatccgc
    36361 gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg acgtctgttt
    36421 atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga acataacgcc
    36481 atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga ccttgagcaa
    36541 aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga gacgccattg
    36601 cgcgatttgg caacaatgac gccggatcaa gcctgccgtc atccgaactg gtcgatgggg
    36661 cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag gtctggaata cattgaagcg
    36721 cgttggctat ttaacgccag cgccagccag atggaagtgc tgattcaccc gcagtcagtg
    36781 attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg ggaaccggat
    36841 atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc tggcgtgaag
    36901 ccgctcgatt tttgcaaact aagtgcgttg acatttgccg caccggatta tgatcgttat
    36961 ccatgcctga aactggcgat ggaggcgttc gaacaaggtc aggcagcgac gacagcattg
    37021 aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc aacaaatccg ctttacggat
    37081 atcgctgcgt tgaatttatc ggtactggaa aaaatggata tgcgcgaacc acaatgtgtg
    37141 gacgatgtgt tatctgttga tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt
    37201 ctcgcaagct gaggataatc cggctacaga gagtctcgct atttgttagc gtagggcttc
    37261 agtgatatag tctgcgccat ctgatcgtaa gtagttggct ttataaggtc aggtatgccg
    37321 tggttttgca cggctttttt tgtataggct tcagtattcc tgagtaccgt aaaccctgtc
    37381 agggaataaa aaacgcgtga tgttgtctgc tactcaacta cttagcgaaa aattgccagc
    37441 gcatggctgc cgtcatgttg cgatcattat ggacggcaat ggtcgctggg caaaaaagca
    37501 agggaagatt cgtgcctttg ggcataaagc cggggcaaaa tccgtccgcc gggctgtctc
    37561 ttttgcggcc aacaacggta ttgaggcgtt aacgctgtat gcctttagta gtgaaaactg
    37621 gaaccgacca gcgcaggaag tcagtgcgtt aatggaactg tttgtgtggg cgctcgatag
    37681 cgaagtaaaa agtctgcacc gacataacgt gcgtctgcgt attattggcg ataccagtcg
    37741 ctttaactcg cgtttgcaag aatgtattcg taaatctgaa gcgctaacag ccgggaatac
    37801 cggtctgacg ctgaatattg cggcgaacta cggtggacgt tgggatatag tccagggagt
    37861 caggcaactg gctgaaaagg tgcagcaagg aaacctgcaa ccagatcaga tagatgaaga
    37921 gatgctaaac cagcatgtct gtatgcatga actggcccct gtagatttag taattaggac
    37981 tgggggggag catcgcatta gtaacttttt gctttggcaa attgcctatg ccgaacttta
    38041 ctttatagat gttctctggc ccgatttcga tgaacaagac tttgaagggg cgttaaatgc
    38101 ctttgctaat cgagagcgtc gtttcggcgg caccgagccc ggtgatgaaa cagcctgatg
    38161 gggggcgctt ttgctgaagt atcgcctgat atctgctttt gtgttaatac ccgtcgtcat
    38221 cgcggcgttg tttctgttgc cgccggtggg gttcgccatt gtaacgctgg tggtctgcat
    38281 gctggcagcg tgggaatggg gacagcttag cggttttacc actcgttcgc agcgagtatg
    38341 gttggcggtg ttatgcgggt tattgttggc gctgatgctt tttctgttgc cggaatatca
    38401 ccgaaatatt catcaaccgc tggttgaaat ctcactttgg gcttcgctgg gttggtggat
    38461 tgtctcgcta ttgctggtgc tgttttaccc aggttccgca gcaatctggc gtaactctaa
    38521 aacattgcgc cttatttttg gcgtgctaac cattgttccc ttcttctggg gcatgctggc
    38581 gttacgggcc tggcactatg acgagaatca ttacagtggc gcaatatggc tgctctatgt
    38641 catgatcctg gtatggggcg ctgactccgg cgcatatatg tttggcaaat tgtttggtaa
    38701 acataagctg gcaccgaagg tttctccggg taaaacctgg caaggcttta taggtggact
    38761 cgctactgca gcggtaatct catggggtta tggcatgtgg gcgaatctcg acgtcgctcc
    38821 cgtcacctta ctcatttgct ctattgtcgc agcgttagcc tcagtgctcg gcgatctgac
    38881 cgagagtatg tttaagcgtg aagcaggaat taaggacagc ggtcatttaa ttccaggaca
    38941 cggtggtatt ttagatcgta ttgatagcct gacggctgcg gtaccggtct ttgcttgctt
    39001 gttgttactg gtattcagga cgctttaacg gaaggtaata tgctgagttt tctctgggat
    39061 ttggcttcgt tcatcgttgc actgggtgta cttatcactg tgcatgaatt tggtcatttc
    39121 tgggttgccc ggcgttgtgg tgttcgcgtt gagcgtttct caatagggtt tggtaaggcg
    39181 ctctggcggc gaactgataa gctcggcacc gaatatgtta tcgccctgat cccgctgggc
    39241 ggttatgtca aaatgctgga tgagcgcgca gaaccggtcg ttccggaact ccgccaccat
    39301 gccttcaata ataaatctgt tggccaacga gcggcgatta ttgccgcagg tccggttgca
    39361 aacttcattt ttgctatctt tgcctactgg ctggttttta ttattggtgt gcctggcgta
    39421 cgtccggtgg ttggtgaaat agcagccaat tcgatagctg cggaagcaca aattgcacca
    39481 ggtacggaac taaaagccgt agatggtatc gaaacgcctg attgggatgc cgtgcgtttg
    39541 cagttggtcg ataaaattgg cgatgaaagc accaccatta cagtagcgcc atttggcagc
    39601 gaccaacggc gggatgtaaa gctcgattta cgtcactggg cgtttgagcc tgataaagaa
    39661 gatccggtat cttcgctggg gattcgtcct cgtgggctgc aaattgaacc tgtactggaa
    39721 aatgtgcagc caaactcggc ggcaagcaag gcaggtttgc aagcaggcga caggatcgtt
    39781 aaagtcgatg gtcagccctt aacgcagtgg gtgacctttg tgatgcttgt ccgggataac
    39841 ccggggaaat ccttagcgtt agaaatcgaa aggcagggaa gtcccttgtc tttgacatta
    39901 atcccggaga gtaaaccggg taatggtaaa gcgattggtt ttgtcggtat tgagccgaaa
    39961 gtcattcctt tgccagatga gtataaagtt gtacgccagt atgggccgtt caacgccatc
    40021 gtcgaagcca cggacaaaac gtggcagctg atgaagctga cggtcagtat gctgggaaaa
    40081 ttgatcaccg gtgatgtgaa actgaacaac ctcagtgggc cgatctctat cgccaagggg
    40141 gctgggatga cagcggaact cggggtagtt tattacctgc cgtttcttgc gcttattagc
    40201 gtgaacttag ggataattaa cctgtttccg ttgcccgtac ttgacggggc gcatctgctg
    40261 ttccttgcga tcgaaaagat caagggcgga ccggtatccg agcgggttca agacttttgt
    40321 tatcgcattg gctcgattct gctggtgctg ttaatggggc ttgcactttt caatgatttc
    40381 tctcggttat gagagttagt taggaagaac gcataataac gatggcgatg aaaaagttgc
    40441 tcatagcgtc gctgctgttt agcagcgcca ccgtatacgg tgctgaaggg ttcgtagtga
    40501 aagatattca tttcgaaggc cttcagcgtg tcgccgttgg tgcggccctc ctcagtatgc
    40561 cggtgcgtac aggcgacacg gttaatgatg aagatatcag taataccatt cgcgctctgt
    40621 ttgctaccgg caactttgag gatgttcgcg tccttcgtga tggtgatacc cttctggttc
    40681 aggtaaaaga acgtccgacc attgccagca ttactttctc cggtaacaaa tcggtgaaag
    40741 atgacatgct gaagcaaaac ctcgaggctt ctggtgtgcg tgtgggcgaa tccctcgatc
    40801 gcaccaccat tgccgatatc gagaaaggtc tggaagactt ctactacagc gtcggtaaat
    40861 atagcgccag cgtaaaagct gtcgtgaccc cgctgccgcg caaccgtgtt gacctaaaac
    40921 tggtgttcca ggaaggtgtg tcagctgaaa tccagcaaat taacattgtt ggtaaccatg
    40981 ctttcaccac cgacgaactg atctctcatt tccaactgcg tgacgaagtg ccgtggtgga
    41041 acgtggtagg cgatcgtaaa taccagaaac agaaactggc gggcgacctt gaaaccctgc
    41101 gcagctacta tctggatcgc ggttatgccc gtttcaacat cgactctacc caggtcagtc
    41161 tgacgccaga taaaaaaggt atttacgtca cggtgaacat taccgaaggc gatcagtaca
    41221 agctttctgg cgttgaagtg agcggcaacc ttgccgggca ctccgctgaa attgagcagc
    41281 tgactaagat cgagccgggc gagctgtata acggcaccaa agtgaccaag atggaagatg
    41341 acatcaaaaa gcttctcggt cgctatggtt atgcctatcc gcgcgtacag tcgatgcctg
    41401 aaattaacga tgccgacaaa accgttaaat tacgcgtgaa cgttgatgcg ggtaaccgtt
    41461 tctacgtgcg taagatccgt tttgaaggta acgatacctc gaaagatgcc gtcctgcgtc
    41521 gcgaaatgcg tcagatggaa ggtgcatggc tgggaagcga tctggtcgat cagggtaagg
    41581 agcgtctgaa tcgtctgggc ttctttgaaa ctgtcgatac cgatacccaa cgtgttccgg
    41641 gtagcccgga ccaagtagat gtagtctaca aggtgaaaga acgtaacacc ggtagcttca
    41701 acttcgggat tggttacggt actgaaagtg gcgtgagctt ccaggctggc gtgcaacagg
    41761 ataactggtt aggtacaggt tatgctgttg gtatcaacgg gaccaaaaac gattaccaga
    41821 cctatgctga attgtcggta accaacccgt acttcactgt agatggtgta agcctcggtg
    41881 gtcgtctctt ctataatgac ttccaggcag atgacgccga cctgtccgac tataccaaca
    41941 agagttatgg tacagacgtg acgttgggct tcccgatcaa cgaatataac tcgctgcgtg
    42001 caggtctggg ttatgtacat aactccctgt ccaacatgca gcctcaggtt gcgatgtggc
    42061 gttatctgta ctctatgggt gaacatccga gcacctctga tcaggataac agcttcaaaa
    42121 cggacgactt cacgttcaac tatggttgga cctataacaa gcttgaccgt ggttacttcc
    42181 cgacagatgg ttcacgtgtc aacctgaccg gtaaagtgac cattcctgga tcggataacg
    42241 aatactacaa agtgacgtta gacacggcga cttatgtgcc gatcgatgac gatcacaaat
    42301 gggttgttct ggggcgtacc cgctggggtt atggtgatgg tttaggcggc aaagagatgc
    42361 cgttctacga gaacttctat gccggtggtt ccagcaccgt ccgtggcttc cagtccaata
    42421 ccattggtcc gaaagcagtt tacttcccgc atcaggccag taattatgat ccggactatg
    42481 attacgaatg tgcgactcag gacggcgcga aagacctgtg taaatcggat gatgctgtag
    42541 gcggtaacgc catggcggtt gccagcctcg agttcatcac cccgacgccg tttattagcg
    42601 ataagtatgc taactcggtt cgtacttcct tcttctggga tatgggtacc gtttgggata
    42661 caaactggga ttccagccaa tattctggat atccggacta tagtgatcca agcaatatcc
    42721 gtatgtctgc gggtatcgca ttacaatgga tgtccccatt ggggccgttg gtgttctcct
    42781 acgcccagcc gttcaaaaag tacgatggag acaaggcaga acagttccag tttaacatcg
    42841 gtaaaacctg gtaagtgttc tccacaaagg aatgtagtgg tagtgtagcg atgactttag
    42901 gcgatcaata taagatcgcc gggccacgca aagaactgca ccctccggtg caaatgggat
    42961 ggtaaggagt ttattgtgaa aaagtggtta ttagcggcag gtctcggttt agcactggca
    43021 acttctgctc aggcggctga caaaattgca atcgtcaata tgggcagcct gttccagcag
    43081 gtagcgcaga aaaccggtgt ttctaacacg ctggaaaatg agttcaaagg ccgtgccagc
    43141 gaactgcagc gtatggaaac cgatctgcag gctaaaatga aaaagctgca gtccatgaaa
    43201 gcgggcagcg atcgcactaa gctggaaaaa gacgtgatgg ctcagcgcca gacttttgct
    43261 cagaaagcgc aggcttttga gcaggatcgc gcacgtcgtt ccaacgaaga acgcggcaaa
    43321 ctggttactc gtatccagac tgctgtgaaa tccgttgcca acagccagga tatcgatctg
    43381 gttgttgatg caaacgccgt tgcttacaac agcagcgatg taaaagacat cactgccgac
    43441 gtactgaaac aggttaaata agtaatgcct tcaattcgac tggctgattt agcgcagcag
    43501 ttggatgcag aactacacgg tgatggcgat atcgtcatca ccggcgttgc gtccatgcaa
    43561 tctgcacaaa caggtcacat tacgttcatg gttaacccaa aataccgtga gcatttaggc
    43621 ttgtgccagg cgtccgcggt tgtcatgacc caggacgatc ttcctttcgc gaaaagtgcc
    43681 gcgctggtag tgaagaatcc ctacctgact tacgcgcgca tggcgcaaat tttagatacc
    43741 acgccgcagc ccgcgcagaa cattgcaccc agtgcggtga tcgacgcgac ggcgaagctg
    43801 gggaacaatg tatcgattgg cgctaacgcg gtgattgaat ccggcgttga actgggcgat
    43861 aacgtgatta tcggtgccgg ttgcttcgta ggtaaaaaca gcaaaatcgg tgcaggttcg
    43921 cgtctctggg cgaacgtaac catttaccat gagatccaga tcggtcagaa ttgcctgatc
    43981 cagtccggaa cagtggtagg cgcagacggc tttggttatg ccaacgatcg tggtaactgg
    44041 gttaagatcc cacagattgg tcgcgtaatt attggcgatc gcgtggagat cggtgcctgc
    44101 actaccatcg atcgcggcgc actggatgac actgttattg gcaatggcgt gatcattgat
    44161 aaccagtgcc agattgcaca taacgtcgtg attggcgaca atacggcggt tgccggtggc
    44221 gtcattatgg cgggcagcct gaaaattggt cgttactgca tgatcggcgg agccagcgta
    44281 atcaacgggc atatggaaat atgcgacaaa gtgacggtta cgggcatggg tatggtgatg
    44341 cgtcccatca ctgaaccagg cgtctattcc tcaggcattc cgctgcaacc caacaaagtc
    44401 tggcgcaaaa ccgctgcact ggtgatgaac attgatgaca tgagcaagcg tctgaaatcg
    44461 cttgagcgca aggttaatca acaagactaa cgttccatct tttgttcgcc aaactttacg
    44521 gcctgtctca ttcttacgat tgcggcaggc cgtgttatta ttgtcgtttc ttatattttg
    44581 acaggaagag tatcttgact actaacactc atactctgca gattgaagag attttagaac
    44641 ttctgccgca ccgtttcccg ttcttactgg tggatcgcgt gctggatttt gaagaaggtc
    44701 gttttctgcg cgcagtaaaa aatgtctctg tcaatgagcc attcttccag ggccatttcc
    44761 ctggaaaacc gattttcccg ggtgtgctga ttctggaagc aatggcacag gcaacaggta
    44821 ttctggcgtt taaaagcgta ggaaaactgg aaccgggtga gctgtactac ttcgctggta
    44881 ttgacgaagc gcgcttcaag cgcccggtcg tgcctggcga tcaaatgatc atggaagtca
    44941 ctttcgaaaa aacgcgccgc ggcctgaccc gttttaaagg ggttgctctg gtcgatggta
    45001 aagtagtttg cgaagcaacg atgatgtgtg ctcgtagccg ggaggcctga tacgtgattg
    45061 ataaatccgc ctttgtgcat ccaaccgcca ttgtggaaga gggcgcgtca attggcgcga
    45121 acgcacacat tggtcctttt tgtatcgttg gaccccatgt cggaattggt gagggtaccg
    45181 tactgaaatc tcacgttgtc gtgaatggtc atactaaaat tggccgcgat aatgagattt
    45241 atcagttcgc ctccatcggc gaagttaacc aggatctgaa atatgctggc gaaccgaccc
    45301 gtgtggaaat cggcgatcgt aaccgcattc gcgaaagcgt caccattcat cgtggcacag
    45361 tccagggcgg tggattgacg aaggtgggca gcgacaactt actgatgatc aacgcgcaca
    45421 ttgcgcacga ttgtacggta ggtaaccgct gtattctcgc caacaacgca acgctggcgg
    45481 gtcacgtatc ggttgacgac ttcgcgatca tcggcggcat gaccgcagtc catcagttct
    45541 gcatcattgg tgcgcacgtg atggttggcg gctgctccgg tgtggcgcag gacgtccctc
    45601 cttatgtcat tgcgcagggt aaccacgcaa cgccgttcgg tgtcaatatc gaagggctga
    45661 agcgccgcgg attcagccgt gaggcgatta ccgctatccg caatgcgtat aagctgattt
    45721 atcgtagcgg taaaacgctc gatgaagtga aaccggaaat tgctgaactg gcggaaacat
    45781 atccggaagt gaaagccttt accgatttct tttcacgctc aacgcgcggt ctgattcgtt
    45841 aatgactgaa cagcgtccat taacgattgc cctggtcgcc ggagaaacct ccggcgatat
    45901 cctgggggcc ggtttaatcc gcgctctgaa agaacgtgtg cccaacgccc gctttgttgg
    45961 tgttgccggg ccacgaatgc aggctgaagg ctgcgaagcc tggtacgaaa tggaagaact
    46021 ggcggtgatg ggcattgttg aagtgctcgg tcgtctgcgt cgcttactgc atattcgtgc
    46081 cgatctgaca aagcgttttg gcgaactgaa gccagatgtt tttgttggta ttgatgcgcc
    46141 tgacttcaat attactcttg aaggtaacct caaaaagcag ggtatcaaaa ccattcatta
    46201 cgtcagtccg tcagtctggg cgtggcgaca gaaacgtgtt ttcaaaatag gcagagccac
    46261 cgatctggtg ctcgcatttc tgcctttcga aaaagcgttt tatgacaaat acaacgtacc
    46321 gtgccgcttt atcggtcata ccatggctga tgccatgcca ttagatccag ataaaaatgc
    46381 cgcccgtgat gtgctgggga tccctcacga tgcccactgc ctggcgttgc taccggggag
    46441 ccgtggtgca gaagttgaaa tgcttagtgc cgatttcctg aaaacggccc agcttttgcg
    46501 ccagacatat ccggatctcg aaatcgtggt gccactggtg aatgccaaac gccgcgagca
    46561 gtttgaacgc atcaaagctg aagtcgcgcg agacttttca gttcatttgc tggatgggat
    46621 gggccgtgag gcgatggtcg ccagcgatgc ggcgctactg gcgtcgggta cggcagccct
    46681 ggagtgtatg ctggcgaaat gcccgatggt ggtgggatat cgcatgaagc cttttacctt
    46741 ctggttggcg aagcggctgg tgaaaactga ttatgtctcg ctgccaaatc tgctggcggg
    46801 cagagagtta gtcaaagagt tattgcagga agagtgtgag ccgcaaaaac tggctgcggc
    46861 gctgttaccg ctgttggcga acgggaaaat cagccacgcg atgcacgata ccttccgtga
    46921 actgcatcag cagatccgct gcaatgccga tgagcaggcg gcacaagccg ttctggagtt
    46981 agcacaatga tcgaatttgt ttatccgcac acgcagctgg ttgcgggtgt ggatgaagtc
    47041 ggacgcgggc cgttagttgg cgcggtcgtc accgctgcgg tgatccttga cccggcgcgc
    47101 ccgattgccg ggctgaatga ttccaaaaag ctgagcgaaa aacgccgtct ggcgctctgt
    47161 gaagagatca aagagaaagc gttgagctgg agtctgggcc gcgcggaacc ccacgaaatc
    47221 gacgagctga acattcttca tgcgaccatg ctggcgatgc agcgtgccgt cgctgggctg
    47281 catattgcgc cggaatatgt gttgattgat ggtaaccgct gcccgaaatt accgatgcct
    47341 gcgatggctg tggtgaaagg cgatagccgc gtaccggaaa tcagtgccgc gtctatcctg
    47401 gcgaaagtga cgcgtgacgc cgaaatggcg gcgctggata ttgttttccc gcaatatggt
    47461 tttgcccaac acaaagggta cccaaccgct tttcatctgg aaaaactggc tgaacacggc
    47521 gcgaccgaac accatcggcg cagctttggg cctgtcaaac gcgcactggg acttgcgtcc
    47581 tgattcttgt gtcgagatta agtaaaccgg aatctgaaga tgtctgaacc acgtttcgta
    47641 cacctgcggg tgcacagcga ctactcgatg atcgatggcc tggccaaaac tgcaccgttg
    47701 gtaaaaaagg cggcggcgtt gggtatgcca gcactggcga tcaccgattt caccaacctt
    47761 tgtggtctgg tgaagttcta cggagcggga catggcgcag ggattaagcc tatcgtcggg
    47821 gcagatttta acgtccagtg cgacctgctg ggtgatgagt taacccacct gacggtactg
    47881 gcggcgaaca ataccggcta tcagaatctg acgttgctga tctcaaaagc gtatcagcgc
    47941 gggtacggtg ccgctgggcc gatcatcgat cgcgactggc ttatcgaatt aaatgaaggg
    48001 ttgatccttc tttccggcgg gcgcatgggc gacgtcggac gcagtctttt gcgtggtaac
    48061 agcgcgctgg tagatgagtg tgtcgcgttt tatgaagaac acttcccgga tcgctatttt
    48121 ctcgagctga tccgcaccgg caggccggat gaagaaagct atctgcacgc ggcggtggaa
    48181 ctggcggaag cgcgcggttt gcccgtcgtg gcgactaacg atgtgcgctt tatcgacagc
    48241 agcgactttg acgcacacga aatccgcgtc gcgatccacg acggctttac cctcgacgat
    48301 cctaaacgcc cgcgtaacta ttcgccgcag caatatatgc gtagcgaaga ggagatgtgt
    48361 gagctgtttg ccgacatccc cgaagccctt gccaacaccg ttgagatcgc caaacgctgt
    48421 aacgtaactg tgcgtcttgg tgaatacttc ctgccgcagt tcccgaccgg ggacatgagc
    48481 accgaagatt atctggtcaa gcgtgcaaaa gagggcctgg aagagcgtct ggccttttta
    48541 ttccctgacg aggaagaacg tgttaagcgc cgcccggaat atgacgaacg tctggagact
    48601 gaacttcagg ttatcaacca gatgggcttc ccgggctact tcctcatcgt tatggaattt
    48661 atccagtggt cgaaagataa cggcgtaccg gtagggccag gccgtggctc cggtgcgggt
    48721 tcactggtgg cctacgcgct gaaaatcacc gacctcgatc cgctggaatt tgacctgctg
    48781 ttcgaacgtt tccttaaccc ggaacgtgtc tccatgcctg acttcgacgt tgacttctgt
    48841 atggagaaac gcgatcaggt tatcgagcac gtagcggaca tgtacggtcg tgatgcggta
    48901 tcgcagatca tcaccttcgg tacaatggcg gcgaaagcgg tgatccgcga cgtaggccgc
    48961 gtgctggggc atccgtacgg ctttgtcgat cgtatctcga aactgatccc gcccgatccg
    49021 gggatgacgc tggcgaaagc gtttgaagcc gagccgcagc tgccggaaat ctacgaagcg
    49081 gatgaagaag ttaaggcgct gatcgacatg gcgcgcaaac tggaaggggt cacccgtaac
    49141 gccggtaagc acgccggtgg ggtggttatc gcgccgacca aaattaccga ttttgcgccg
    49201 ctttactgcg atgaagaggg caaacatccg gtcacccagt ttgataaaag cgacgttgaa
    49261 tacgccggac tggtgaagtt cgacttcctt ggtttgcgta cgctcaccat catcaactgg
    49321 gcgctggaga tgatcaacaa gcggcgggcg aagaatggcg agccgccgct ggatatcgcc
    49381 gcgatcccgc tggatgacaa gaaaagcttc gacatgctgc aacgctcgga aaccacggcg
    49441 gtattccagc ttgaatcgcg cggcatgaag gatctgatca agcgtctgca acctgactgc
    49501 ttcgaagata tgatcgcact ggtggcgctg ttccgcccag gtccgttgca atcagggatg
    49561 gtggataact ttatcgaccg taaacatggt cgcgaagaaa tctcctatcc ggacgtacag
    49621 tggcagcatg aaagcctgaa accggtactg gagccaacct acggcatcat cctgtatcag
    49681 gaacaggtca tgcagattgc gcaggtgctt tctggttata ccctcggtgg cgcggatatg
    49741 ctgcgtcgtg cgatgggtaa gaaaaagccg gaagagatgg ctaagcagcg ttctgtattt
    49801 gctgaaggtg cagaaaagaa cggagtcaac gccgaactgg cgatgaaaat cttcgacctg
    49861 gtggagaaat tcgcgggtta cggatttaac aaatcgcact ctgcggccta tgctttggtg
    49921 tcatatcaaa cgttatggct gaaagcgcac tatccggcgg agtttatggc ggcggtaatg
    49981 accgccgata tggacaacac cgagaaggtg gtgggcctgg tggatgagtg ctggcggatg
    50041 gggctgaaaa tcctgccacc agatataaac tccggtcttt accatttcca cgtcaacgac
    50101 gacggcgaaa tcgtgtatgg tattggcgcc atcaaagggg taggtgaagg tccgattgag
    50161 gccatcatcg aagcccgtaa taaaggcggc tacttccgcg aactgtttga tctctgcgcc
    50221 cgtaccgaca ccaaaaagtt aaaccggcgg gtgctggaaa aactgatcat gtccggggcg
    50281 tttgaccgtc ttgggccaca ccgcgcggcg ctgatgaact cgctgggtga tgcgttaaaa
    50341 gcggcagatc aacacgcgaa agcggaagct atcggtcagg ccgatatgtt cggcgtgctg
    50401 gccgaagagc cggaacaaat tgaacaatcc tacgccagct gccaaccgtg gccggagcag
    50461 gtggtgttag atggggaacg tgaaacgtta ggtctgtacc ttacgggaca ccctatcaac
    50521 cagtatttaa aagagattga gcgttatgtc ggaggcgtaa ggctgaaaga catgcacccg
    50581 acagaacgtg gtaaagtcat cacggctgcg gggctcgttg ttgccgcgcg ggttatggtc
    50641 accaagcgcg gcaatcgtat cggtatctgc acgctggatg accgttccgg gcggctggaa
    50701 gtgatgttgt ttactgacgc cctggataaa taccagcaat tgctggaaaa agaccgcata
    50761 cttatcgtca gcggacaggt cagctttgat gacttcagcg gtgggcttaa aatgaccgct
    50821 cgcgaagtga tggatattga cgaagcccgg gaaaaatatg ctagcgggct tgctatctcg
    50881 ctgacggaca ggcaaattga tgaccagctt ttaaaccgac tccgtcagtc tctggaaccc
    50941 caccgctctg ggacaattcc agtacatctc tactatcaga gggcggatgc acgcgcgcgg
    51001 ttgcgttttg gcgcgacgtg gcgtgtctct ccgagcgatc gtttattaaa cgatctccgt
    51061 ggcctcattg gttcggagca ggtggaactg gagtttgact aatacaggaa tactatgagt
    51121 ctgaatttcc ttgattttga acagccgatt gcagagctgg aagcgaaaat cgattctctg
    51181 actgcggtta gccgtcagga tgagaaactg gatattaaca tcgatgaaga agtgcatcgt
    51241 ctgcgtgaaa aaagcgtaga actgacacgt aaaatcttcg ccgatctcgg tgcatggcag
    51301 attgcgcaac tggcacgcca tccacagcgt ccttataccc tggattacgt tcgcctggca
    51361 tttgatgaat ttgacgaact ggctggcgac cgcgcgtatg cagacgataa agctatcgtc
    51421 ggtggtatcg cccgtcttga cggtcgtccg gtgatgatca ttggtcatca aaaaggtcgt
    51481 gaaaccaaag aaaaaattcg ccgtaacttt ggtatgccag cgccagaagg ttaccgcaaa
    51541 gcactgcgtc tgatgcaaat ggctgaacgc tttaagatgc ctatcatcac ctttatcgac
    51601 accccggggg cttatcctgg cgtgggcgca gaagagcgtg gtcagtctga agccattgca
    51661 cgcaacctgc gtgaaatgtc tcgcctcggc gtaccggtag tttgtacggt tatcggtgaa
    51721 ggtggttctg gcggtgcgct ggcgattggc gtgggcgata aagtgaatat gctgcaatac
    51781 agcacctatt ccgttatctc gccggaaggt tgtgcgtcca ttctgtggaa gagcgccgac
    51841 aaagcgccgc tggcggctga agcgatgggt atcatcgctc cgcgtctgaa agagctgaaa
    51901 ctgatcgact ccatcatccc ggaaccgctg ggcggcgctc accgtaaccc ggaagcgatg
    51961 gcggcatcgt tgaaagcgca actgctggcg gatctggccg atctcgatgt gttaagcact
    52021 gaagatttaa aaaatcgtcg ttatcagcgc ctgatgagct acggttacgc gtaattcgca
    52081 aaagttctga aaaagggtca cttcggtggc ccttttttat cgccacggtt tgagcaggtt
    52141 atgattaagg aaggattttc caggaggaac acatgaacat cattgccatt atgggaccgc
    52201 atggcgtctt ttataaagat gagcccatca aagaactgga gtcggcgctg gtggcgcaag
    52261 gctttcagat tatctggcca caaaacagtg ttgatttgct gaaatttatc gaacataacc
    52321 ctcgaatttg cggcgtgatt tttgactggg atgagtacag tctcgattta tgcagcgata
    52381 tcaatcaact taatgaatat ctcccgcttt atgccttcat caacacccac tcgacgatgg
    52441 atgtcagcgt gcaggatatg cggatggcgc tctggttttt tgaatatgcg ctggggcagg
    52501 tggaagatat cgccattcgt atgcgtcagt acaccaacga atatcttgat aacattacgc
    52561 cgccgttcac gaaagccttg tttacctacg ttaaagagcg gaagtacacc ttttgtacgc
    52621 cggggcatat gggcggcacc gcatatcaaa aaagcccggt tggctgcctg ttttatgatt
    52681 ttttcggcgg gaataccctc aaggctgatg tctctatttc ggtcaccgag cttggttcgt
    52741 tgctcgacca caccgggcca cacctggaag cggaagagta catcgcgcgg acttttggcg
    52801 cggaacagag ttatatcgtt accaacggaa catcgacgtc gaacaaaatt gtgggtatgt
    52861 acgccgcgcc atccggcagt acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc
    52921 atctgttgat gatgaacgat gtagtgccag tctggctgaa accgacgcgt aatgcgttgg
    52981 ggattcttgg tgggatcccg caccgtgaat ttactcgcga cagcatcgaa gagaaagtcg
    53041 ctgccaccac gcaggcacaa tggccggttc atgcggtgat taccaactcc acctatgatg
    53101 gcttgctcta caacaccgac tggatcaaac agacgctgga tgtcccgtcg atccacttcg
    53161 attccgcctg ggtgccgtac acccattttc atccgatcta ccaggggaaa agtgatcttc
    53221 gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc aggcttcgct gatccacatt
    53281 aaaggcgagt atgacgaaga ggcgtttaac gaagccttta tgatgcatac caccacctcg
    53341 cccagttatc ccattgttgc ttcggttgag acggcggcgg cgacgctgcg tggtaatccg
    53401 ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc attttcgcaa agaggtccag
    53461 cggttgcggg aagagtctga cggttggttt ttcgatatct ggcaaccgcc gcaggtggat
    53521 gaagccgaat gctggcccgt tgcgccaggc gaacagtggc acggctttaa cgatgcggat
    53581 gccgatcata tgtttctcga tccggttaaa gtcactattt tgataccggg gatggacgaa
    53641 cagggcaata tgagcgagga ggggatcctg gcggcgctgg tggcaaaatt cctcgacgaa
    53701 cgtgggatcg tagtagagaa aaccggccct tataacctgc tgtttctctt tagtattggt
    53761 atcgataaaa ccaaagcaat gggattattg cgtgggttga cggaatttaa acgctcttac
    53821 gatctcaacc tgcggatcaa aaatatgcta cccgatctct atgctgaaga tcccgatttc
    53881 taccgcaata tgcgtattca ggatctggcg caagggatcc ataagctgat tcgtaaacac
    53941 gatcttcccg gtttgatgtt gtgggcattc gatactttgc cggaaattat catgacgcca
    54001 catcaggcat ggcagagaca gattaaaggt gaagtagaaa ccattgcgct ggaacaactg
    54061 gtcggtagag tatcggcaaa tatgatcctg ccttatccac cgggcgtacc gctgttgatg
    54121 ccaggagaaa tgctgaccaa agagagccgc acggtactcg attttctact gatgctttgt
    54181 tccgtagggc aacattaccc cggttttgaa acggatattc acggcgcgaa acaggacgaa
    54241 gacggcgttt accgcgtacg agtcctaaaa atggcgggat aacttgccag agcggcttac
    54301 gggcgagtaa cgtgctgtta acaaataaag gagacgttat gctgggttta aaacaggttc
    54361 accatattgc gattattgcg acggattatg cggtgagcaa agctttctac tgcgatattc
    54421 ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg aaaggggatt
    54481 tggcgcttaa tgggcaatat gtgattgagc ttttctcttt tccgttcccg ccggagcgcc
    54541 ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat gacatcgatg
    54601 cggcagtggc gcaccttgaa agccataacg tgaagtgtga agccatccgt gtcgatccat
    54661 acacgcaaaa acgcttcacc ttctttaacg atccggacgg gctgccgttg gaactgtatg
    54721 agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt gctactgtaa
    54781 aatcgcacca tcatgacact cacgctcaat agacaacttc tcacctcacg ccagattttg
    54841 gtcgccttta gcggcgggct tgactccacc gttctgctgc atcagttggt gcagtggcgg
    54901 acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc atcacggttt aagtgccaat
    54961 gccgatgcct gggttacgca ttgcgaaaac gtctgccaac agtggcaggt gccgctggtg
    55021 gtcgaacgcg tacaacttgc gcaagagggg ctggggattg aggcccaggc gcggcaggcg
    55081 cgttatcagg catttgcccg taccctattg cccggcgaag tgctggtcac cgcgcaacat
    55141 ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg ccctgccggg
    55201 ctttcggcta tggcggaagt ttcggagttt gccggaacac ggcttatccg cccgttgctt
    55261 gcccgcacgc ggggggaact ggcgcagtgg gcattggcgc atggtttacg ctggattgaa
    55321 gacgaaagta atcaggacga ctcatacgat cgtaactttc tgcgcctgcg cgtagtgccg
    55381 ttattgcagc agcgttggcc gcattttgcg gaagcaacgg cccgcagcgc cgcactttgt
    55441 gctgaacaag agagtctgtt ggatgaactg ctggcggatg atttagcaca ctgccaaacg
    55501 tcgcagggga cgctgcagat tgcgccaatg ctggcgatga gtgatgcccg ccgcgcggcg
    55561 attatccgtc gctggctggc agggcagaat gcaccaatgc cttcccgcga cgcgctggtg
    55621 cggatctggc aggaagtggc gctggcgcgg gaagatgcct ccccctgttt acgcttgggc
    55681 gcgtttgaaa tccgccgcta tcagtcgcaa ctgtggtgga ttaaatccgt caccgggcaa
    55741 agtgaaacca ttgtgccgtg gcagacgtgg ctacaaccgc tggaattacc ggcggggctg
    55801 ggaagcgtac agcttactgc gggaggcgat attcgccctc cgcgtgcaga cgaagcggtc
    55861 agcgtgcgtt tcaaatcggc aggattgctg catattgtcg ggcgcaacgg cggacgtaag
    55921 ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc tacgtgacac cacgccactg
    55981 ctgttttatg gcgaaacgct gattgcggcg gcaggggtat ttgtgacgca agaaggtgtg
    56041 gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa cgcttagtta agtgaaagcc
    56101 ggataagacg catcaaacgt cgcatccggc gaaagtcaat caggactcgc tcaccaccac
    56161 cgtaccgatt tccgggtggc taaagctggt aattttatcc agacgtagct cacgggtttc
    56221 gccagcggcc tcgacgacca gatactccac atttttgcgg gagactaaat cgctggcttt
    56281 tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc attaaatgat gctggcaggc
    56341 gagctcaaga ttatcgtaat catcacaatt gattggttga tacgtatcat tcattaacat
    56401 aatcgctcac cagtaagttt gccgcagcgt atgctgcttt ttccctgaca gcctcagaaa
    56461 gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac gcagcccagc gcgtccggaa
    56521 cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt gcgtattaac tcacaatatt
    56581 tttccacatg ccctcctgtc agcacactga cttaaccgtg gatgcaagtc taagcctacg
    56641 aagataaact ctgtttcgca aggtgactat accacactca tttctgcaat atcagcgccg
    56701 caactgcacg tattccgtta caatggcctc ctgattcgaa aggagttttc ttatggcgct
    56761 taaagcgaca atttataaag cgacggttaa tgtggccgat ctcgaccgca accagtttct
    56821 cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta tgatgctgcg
    56881 cctgctggcg tggctgaaat atgctgatga acgtctgcaa tttacccgtg gtttgtgtgc
    56941 cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt tgtggattga
    57001 gctggggctg ccggatgagc ggcggattaa gaaagcctgc acccaggccg cagaagtggc
    57061 gctgtttacc tataatagtc gggcggcgca aatctggtgg cagcaaaatc agagcaaatg
    57121 tgtgcagttt gccaatcttt ccgtctggta tctggacgat gaacaactgg cgaaagtaag
    57181 cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt caggatggcg tgatctggtt
    57241 atcggacgat aagaataatc tggaagttaa cttaaccgcc tggcaacaac cttcatgatt
    57301 gtgatttccc gacatgttgc tattcccgat ggtgagcttg agatcaccgc cattcgtgcg
    57361 cagggcgcgg gcgggcagca tgttaataag acctcaacgg ccattcatct gcgttttgac
    57421 attcgggcgt ccagcctgcc agagtattac aaagagcgtc tgctcgccgc cagccatcat
    57481 ttgatcagca gtgatggcgt gattgtcatt aaggcacagg aataccgcag tcaggaactg
    57541 aaccgcgaag ccgcgctagc ccggctggtg gcagtgatta aagatttaac aacagaacaa
    57601 aaagcccgac gacccacgcg gcccacccgt gcatcgaaag agcgcaggct ggcatcgaaa
    57661 gcacaaaaat caagcgcgaa ggcgatgcgc ggcaaagtac gcagcggtcg ggaataaaaa
    57721 gaaggaatag atggtgaaaa aagcgatagt gacagcgatg gctgtaatca gcctctttac
    57781 tctgatggga tgtaataacc gggccgaagt cgatacgctt tctccagcac aggctgccga
    57841 actgaaaccg atgccgcaaa gttggcgcgg cgtgctgccg tgtgctgatt gtgaaggaat
    57901 cgaaacctct ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc gttatctcgg
    57961 tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa ccgctgacaa
    58021 gctggtatta accgacagca aaggtgaaaa gtcatattat cgggcgaaag gcgatgcgct
    58081 ggagatgctc gatcgtgaag gcaatccgat tgaatcgcag ttcaactata cgctggaacc
    58141 ggcacaatcc agtttaccta tgacgccgat gaccctgcga ggcatgtatt tttatatggc
    58201 tgatgcggcg accttcactg attgcgcgac cggaaaacgt ttcatggtag cgaataacgc
    58261 agagctggag cgtggctacc tggctgcgcg cggtcacagt gaaaaaccgg tgttactgtc
    58321 agtagaaggt cactttacgc ttgaggctaa tccggatact ggtgcgccaa ccaaagtatt
    58381 agcgcccgat acggcgggta aattttaccc gaataaggat tgcagtagtt tggggcagta
    58441 aacctgctta aggcagaaac aaacgcaaaa caggccagaa ggatatattt caacattttg
    58501 aatttgcacg ttttttgtag gccggataag gcgtttacgc cgcatccggc aatggtgctc
    58561 aacgcctgat gcgacgctgg cgcgtcttat catgcctaca acccccctca taccttaagg
    58621 cggataaggc atttacttta tcaccgggtc aaaccaacaa agcgacccgc cttgatataa
    58681 atccctggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa cgcagagaca
    58741 tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg cgccagtgta
    58801 agtggatgtc cggctttggc aaatgcatca gtgacaaatt ccgaacaaaa ccacgacttt
    58861 ttgtctcctt cgcccacact gcttaactgc gctttcgcca ggccgctgac gcactgctgg
    58921 cgaaaatcct cggagaactg attcagtgag cacatctggc gagtcaccat aaagggaata
    58981 aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc aaacgcggtg
    59041 atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag cttatcacta
    59101 tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc ttctgcaacg
    59161 ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa gacgcggatt
    59221 ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt gagatcaggt
    59281 gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa tttgacagcc
    59341 catgtttttg cctcggcatc tactgctgtg gctgatgggg ctggctgata tcaacggtac
    59401 aagccgaaag taaaagaaag ctggggagaa gcaggcggca gtacgccttt ggtttatcca
    59461 ttttatacaa tccatgtaaa aaagggccct gaaattcagg accctttctg gcatcagcct
    59521 ttaatctgtt tcaccagata ttcgacgatg tcaccagtct taattaactg tttctcgccg
    59581 ttgcgacgat atttatattc gatatcgtcg ttgtcgaggt tacggtcgcc cagcacaata
    59641 gtgtgcggaa taccgatcag ttccatatca gcaaacatca cgcccggacg ctctttgcgg
    59701 tcatccagca gcacttcgat accttgtgca cgcagttcgc tgtacagttt ctcagcaagc
    59761 tcttgtacgc ggaaggattt gtgcatgttc atcggcagaa tcgccacctg gaacggcgcg
    59821 atagcgtcag gccatacgat gccgcgttcg tcgtagttct gctcaatcgc cgcagctacc
    59881 acacgcgtta ccccgatacc gtagcaaccc atcgtcagga tttggttacg gccatcttca
    59941 ccctgtacgg aggctttcag tgcttcggag tacttggtac ccagctggaa gatgtgacca
    60001 acttcgatac cacgtttgat cagcagcgta ccctggccat ccgggcttgg atcgccagcc
    60061 accacgttac ggatatctgc aacttccggg gtagcgacat cgcgatccca gttgataccg
    60121 aagtagtgtt taccatcgat gttagcacca gcagcgaaat cactcatcgc cgcaacggta
    60181 cggtcaatca ccaccggaat cggcatgttt accggaccca gtgaacccgg accggcttta
    60241 accacagcac gaatttcttc ttcggtcgcg aaagtcagcg ggcttgcaac ctgcggcagt
    60301 ttttctgctt taacttcgtt cagctcgtga tcaccgcgca ccagcagcgc aaccagcggg
    60361 aagctgctgc cttcaaccgc tttaaccagc agagtcttaa ccgttttctc aatcggcaga
    60421 ttgaactgtt caaccagttc cgcgatggtt ttcgcgttcg gcgtatcaac cagcgtcatt
    60481 tcctgggtag cagcagcgcg cggttctttc ggcgcgatag cttctgccag ttcaatgttc
    60541 gctgcatagt cagaggtgtc ggagaagacc acatcgtctt caccgctctg cgccagcacc
    60601 tggaattcgt gagaggcgct gccgccgata gaaccggtgt cggcttgcac ggcgcggaaa
    60661 tccagcccca tgcggctgaa gattttgctg taggccgcat acattgcatc gtaggtttcc
    60721 tgcagggatt cctgagaagt atggaaagag taagcatctt tcatcaggaa ttcgcgggaa
    60781 cgcatgacgc cgaaacgcgg acgcacttcg tcgcggaact tggtctggat ctgatagaag
    60841 ttcagcggca gctgtttgta agagctaagc tcgttacgaa tcaggtcagt gataacttct
    60901 tcatgagttg ggccgagtac gaacggacgc tcgccacggt caacaaaacg cagcagttcc
    60961 ggaccgtact gttcccaacg accactctct tgccacaaat cggctggctg aaccaccggc
    61021 atcgacacct cgattgcacc ggcgttgttc atctcttcac gcacgatgtt ttcgactttt
    61081 ttcagaacgc gcacgccggt cggcagccag gtatataacc cggaggccag cttgcggatc
    61141 atcccggcgc gcagcatcag ctgatggctg atcacctcgg cgtcggcagg tgtctccttg
    61201 agagtggaga gcaggtattg gctagtacgc atgttgttac ggttccagtt ggaaggtaga
    61261 acaggctcaa ggcgagcctg ggacaaaaaa agtgatttag tttaccagtg caaaagaaat
    61321 gtcaaaagag aagggcgtga atttaacgcg gttccagcgc aaagacttca aaacctgcgt
    61381 cggtgacgcg ccagcgaacg ttaaaatcat gtagccagac ggcataggtt ttgcccgttt
    61441 cctcaccttt atgataggcc gggcgcgggt cctgcgccag tacttcgcgg ataaacagcg
    61501 ttaactgcgg ataacgcttc tccagcgtca aaagctgctt ttcgacctct gcggtaaaac
    61561 tcaccgccat ctctgcagct ggcgcacttt gcgcatagct ggcactggca tcgggaagcg
    61621 attcggcaaa ggggagatac ggtttgatat ccactaccgg cgtaccatcg accagatcca
    61681 gactgccgag cttcagaatc acgctgtctt tatggcaaac aacctctttc agctctacca
    61741 gcgacatacc aattgggtta gggcggaaag tagagcgtgt tgcgaaaacc cccattctgg
    61801 cgttaccgcc gaggcgcggt ggacgcacag tcggacgcca gccgccttcc atcgtttgat
    61861 gaaagacgaa aaggatccat aaatggctga acgcttccag gccgcgaacg gcgtcggcct
    61921 ggttgtaggg agcaatgaga tgcagttcgc cgttggcgct ttttaccaga cctggctggc
    61981 gcggaacggc gaatttttct ttatagggcg agcgaataac gcctatttgc tcgaactgga
    62041 aactgctcat ttcgccgtaa tgttaagcgc agagccgata catacagcct gacgatagca
    62101 gcctggcgta ccgctggtga cttcgcagct atgcagtaat accgcattgg ctttcatttt
    62161 agaggcgttg atttgcatcc gcttacgcgc ggttggaatg ctcggcggag agtcctgatt
    62221 agaggcctgg caagagtcgc cactgacttc accgagatcg cggaacggtt tgccgactaa
    62281 ttcttctgca ttggtataaa ttcggaccgg cgtggcgcgc ggcgctttcg gttttgcagg
    62341 ctccgctttc ggctggggtg cagtgctttg aacgggttcg acaggggatc tgcttaacat
    62401 ggaacagccg cttagcatga gtgctactaa acagatcggt aaagcacgca tagtatttcc
    62461 tcaatgtatg atcaaaacgt caatattgaa tcaggagctt gtaaaaatga caagacgggc
    62521 aagtgcccat cctgaatgat attacaaatt gtggaaacag cctaaaaatt accagccttt
    62581 aacagcgccg ccgttaaaca ctttgtttgc tgcttcgtaa acttcatcag actgataagc
    62641 ctggacgaat ttcttcacgt tctcggcgtc tttgttatct tcacgcgtta cgatcaggtt
    62701 tacgtacggg gagtctttat cttcgacaaa gataccgtct ttcgctggag tcaggccaat
    62761 ctggctggca taggtggtat tgataactgc cagagcgatt tgtgcgtcgt ccagagatcg
    62821 aggcagctgc ggtgcttcca gctcaacaat tttcagattt tttgggttct caacaacatc
    62881 aagtacggtc ggcagcaggc caacgccatc tttcagtttg atcaagccca ctttttgcag
    62941 cagcagcagt gaacgaccaa ggttagttgg atcgtttggc acggcaacct gcgaaccatc
    63001 ctgcagttca tccagtgatt tgattttctt ggagtaacca gcaatcggat aaacaaaagt
    63061 gttgcctact gcgaccagtt tgtagccacg atctttcagt tgctgatcaa ggtacggttt
    63121 atgctggaag gcgttggcgt cgatatcgcc tttgctcaat gcttcgtttg gcagaacata
    63181 gtcgttgaag gttaccagct caacgtccag gccatatttg tctttcgcaa ctttctgcgc
    63241 gacttctgca acctgctgtt cggcaccaac aatcacgccg actttaatgt ggtttggatc
    63301 tttttcatcc tgaccgcagc ctaccagtgc cagtgatcca atcagggctc ccactgccgc
    63361 aaaggttttg aatttgaacg ccatacctta ttccttcttc aattatttat gttgtgttga
    63421 acgttacttg cgagtgacag cccggacgat gcggtcgcct gcgaactgaa ttaaataaac
    63481 cagaatgacc agcaatacca gtaccgtatt catcaccgtc gcgttatagc cgatgtagcc
    63541 atactgatag ccaatctgac ctaaaccacc ggcaccgaca gcaccaccca tcgcggaata
    63601 accgactagg gtaatcaggg tgatagttgc cgcattcacc agacccggca atgcttccgg
    63661 taacagcact ttacggacga tctgcatcgg cgtggcaccc attgcacggg aagcttcaat
    63721 taacccggtt gggatctcca gcagagcgtt ctcgaccata cgggcaataa acggtgctgc
    63781 accaacggtt aacggaacaa tcgctgcctg caaaccaatc gatgtaccaa caataacgcg
    63841 ggtaaacgga atcatccata ccagcaagat aatgaacggg atagaacgga aaatgttcac
    63901 aatcgcagaa acggtacgat acagcttcgc gttagcaata atttgccccg gacgcgtgac
    63961 ataaagcaga acgccaaccg gcagaccaat cacaaagcca aaaaaaccgg atacgaaggt
    64021 cattgccagc gtttcccata cgccacgaac cagcagccac atcatcggct cagacataac
    64081 ccagtacctc tacttttaca tggtgttcct gcagccaggc aatggcggct tgcgtatctt
    64141 gttgtgtgcc gtgcatttca gtcagcatga tgccgaactt aacgccaccg gcgtagtcca
    64201 tctgcgcgct aataatgttg ttgttgacgt tgaaacgacg cgcggtttca gaaagcagtg
    64261 gggcatcgac cgattgaccg gtaaactcca gacgcagcat cggcacgcag tcagtaaatg
    64321 gctccgcttg cagacgttcc tggtaatctt ccgggatatc cagatgcagg gtcgactgaa
    64381 taaacttctg cgccagcggc gttttcggat gcgagaacac ttcacttacc gtgtcctgct
    64441 cgatcagttc gccattgctg atgaccgcca cgcaatcaca aatacgcttc acaacgtcca
    64501 tttcgtgggt gatcaacaga atcgtcaaac ccagacggcg gttaatgtct ttcagcagtt
    64561 cgagaataga acgtgtcgtt gccgggtcca gcgcgctggt ggcttcatca cacagcaata
    64621 ctttgggatt gctggctaat gcacgggcaa tcgccacacg ttgtttctgc ccaccggaaa
    64681 gattcgacgg gtagctatca tgcttatcgc caagaccaac caatgacagc aattccgtca
    64741 cgcgacgttt gatctcgtct ttcggtgtgt tgtccagctc cagcggcaga gccacgttgc
    64801 caaaaacagt acgcgaagag agcaggttaa aatgctggaa aatcatacca atctggcggc
    64861 gagctttggt caactcggat tctgacagcg tggtcagttc ctggccatcg accagcacgc
    64921 taccctcggt tgggcgctcc agcaggttta cacaacgtat aagcgtactc ttacccgcgc
    64981 ctgaggcacc gataacgcca taaatttgtc cagctggcac atgcaggctg acgttgttca
    65041 acgcctggat ggtgcgggtg ccctggtgga acactttggt gatattcgaa agttttatca
    65101 ttgattattt attatcgtca ttaagttagt cgtggcatct cgaatgcctg aaacgggcaa
    65161 cgccgtcaat gaaatggatg ttaaggcatc cagacgtcta aatcaatcag gtttatgcga
    65221 agagcacttt cttgcaggtc gaaacatgcg atactagcgt cacatgcctt attaaggagc
    65281 tataaaaggt ggcaaagagc gtacccgcaa tttttcttga ccgtgatggc accattaatg
    65341 tcgatcacgg ctatgtccat gagatcgaca actttgaatt tatcgacggt gttattgacg
    65401 ccatgcgcga gctaaaaaaa atgggctttg tgctggtggt agtaaccaac cagtctggca
    65461 ttgctcgcgg taaatttacc gaagcacagt ttgaaacgct gaccgagtgg atggactggt
    65521 cgctggcgga ccgagatgtc gatctggatg gtatctatta ttgcccgcat catccgcagg
    65581 gtagtgttga agagtttcgc caggtctgcg actgccgtaa accacatccg gggatgcttt
    65641 tgtcagcacg cgattatttg catattgata tggccgcttc ttatatggtg ggcgataaat
    65701 tagaagatat gcaggcagcg gctgcggcga acgtgggaac aaaagtgctg gtgcgtacgg
    65761 gtaaacctat tacgcctgaa gcagaaaacg cggcggattg ggtgttaaat agcctggcag
    65821 acctgccgca agcgataaaa aagcagcaaa aaccggcata atgattaaaa gatgagcgat
    65881 tgaaataaaa atgcattttt ccacttgtct tcctgagacg actccctata atgcgcctcc
    65941 atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttga agagaaaaat
    66001 cctgaaattc agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt
    66061 gcgctaaagt gcgtcgcaac tgctctttaa caatttatca gacaatctgt gtgggcactc
    66121 gaagatacgg attcttaacg tcgcaagacg aaaaatg
//
LOCUS       DAJVOY010000004        64690 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVOY010000004 DAJVOY010000000
VERSION     DAJVOY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.004"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(188..1420)
                     /gene="araJ"
                     /locus_tag="OQL90_000238"
     CDS             complement(188..1420)
                     /gene="araJ"
                     /locus_tag="OQL90_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1367138.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAGE
                     TTGVGYSLGVHYLLPDAA"
     gene            complement(1546..4689)
                     /gene="sbcC"
                     /locus_tag="OQL90_000239"
     CDS             complement(1546..4689)
                     /gene="sbcC"
                     /locus_tag="OQL90_000239"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1367139.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(4686..5888)
                     /gene="sbcD"
                     /locus_tag="OQL90_000240"
     CDS             complement(4686..5888)
                     /gene="sbcD"
                     /locus_tag="OQL90_000240"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1367140.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGTVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            6078..6767
                     /gene="phoB"
                     /locus_tag="OQL90_000241"
     CDS             6078..6767
                     /gene="phoB"
                     /locus_tag="OQL90_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS1367141.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6825..8120
                     /gene="phoR"
                     /locus_tag="OQL90_000242"
     CDS             6825..8120
                     /gene="phoR"
                     /locus_tag="OQL90_000242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1367142.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            8527..9846
                     /gene="brnQ"
                     /locus_tag="OQL90_000243"
     CDS             8527..9846
                     /gene="brnQ"
                     /locus_tag="OQL90_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1367143.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            9922..11295
                     /gene="proY"
                     /locus_tag="OQL90_000244"
     CDS             9922..11295
                     /gene="proY"
                     /locus_tag="OQL90_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1367144.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            11451..13173
                     /gene="malZ"
                     /locus_tag="OQL90_000245"
                     /pseudo
     CDS             11451..13173
                     /gene="malZ"
                     /locus_tag="OQL90_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(13178..13759)
                     /gene="acpH"
                     /locus_tag="OQL90_000246"
     CDS             complement(13178..13759)
                     /gene="acpH"
                     /locus_tag="OQL90_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCS1367145.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSGQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            13852..14922
                     /gene="queA"
                     /locus_tag="OQL90_000247"
     CDS             13852..14922
                     /gene="queA"
                     /locus_tag="OQL90_000247"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1367146.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTSVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            14978..16105
                     /gene="tgt"
                     /locus_tag="OQL90_000248"
     CDS             14978..16105
                     /gene="tgt"
                     /locus_tag="OQL90_000248"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1367147.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            16128..16460
                     /gene="yajC"
                     /locus_tag="OQL90_000249"
     CDS             16128..16460
                     /gene="yajC"
                     /locus_tag="OQL90_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1367148.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            16488..18335
                     /gene="secD"
                     /locus_tag="OQL90_000250"
     CDS             16488..18335
                     /gene="secD"
                     /locus_tag="OQL90_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1367149.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            18346..19317
                     /gene="secF"
                     /locus_tag="OQL90_000251"
     CDS             18346..19317
                     /gene="secF"
                     /locus_tag="OQL90_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1367150.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGEDLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            19446..19793
                     /locus_tag="OQL90_000252"
     CDS             19446..19793
                     /locus_tag="OQL90_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1367151.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(19970..20854)
                     /gene="tsx"
                     /locus_tag="OQL90_000253"
     CDS             complement(19970..20854)
                     /gene="tsx"
                     /locus_tag="OQL90_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1367152.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTDLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(21153..21692)
                     /locus_tag="OQL90_000254"
     CDS             complement(21153..21692)
                     /locus_tag="OQL90_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1367153.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPRAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            21843..22292
                     /gene="nrdR"
                     /locus_tag="OQL90_000255"
     CDS             21843..22292
                     /gene="nrdR"
                     /locus_tag="OQL90_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1367154.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            22296..23399
                     /gene="ribD"
                     /locus_tag="OQL90_000256"
     CDS             22296..23399
                     /gene="ribD"
                     /locus_tag="OQL90_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1367155.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            23488..23958
                     /gene="ribE"
                     /locus_tag="OQL90_000257"
     CDS             23488..23958
                     /gene="ribE"
                     /locus_tag="OQL90_000257"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1367156.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            23978..24397
                     /gene="nusB"
                     /locus_tag="OQL90_000258"
     CDS             23978..24397
                     /gene="nusB"
                     /locus_tag="OQL90_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1367157.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            24475..25452
                     /gene="thiL"
                     /locus_tag="OQL90_000259"
     CDS             24475..25452
                     /gene="thiL"
                     /locus_tag="OQL90_000259"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1367158.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            25430..25948
                     /gene="pgpA"
                     /locus_tag="OQL90_000260"
     CDS             25430..25948
                     /gene="pgpA"
                     /locus_tag="OQL90_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1367159.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(26002..26976)
                     /gene="yajO"
                     /locus_tag="OQL90_000261"
     CDS             complement(26002..26976)
                     /gene="yajO"
                     /locus_tag="OQL90_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1367160.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(27031..28893)
                     /gene="dxs"
                     /locus_tag="OQL90_000262"
     CDS             complement(27031..28893)
                     /gene="dxs"
                     /locus_tag="OQL90_000262"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1367161.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(28918..29817)
                     /gene="ispA"
                     /locus_tag="OQL90_000263"
     CDS             complement(28918..29817)
                     /gene="ispA"
                     /locus_tag="OQL90_000263"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1367162.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            complement(29817..30059)
                     /gene="xseB"
                     /locus_tag="OQL90_000264"
     CDS             complement(29817..30059)
                     /gene="xseB"
                     /locus_tag="OQL90_000264"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1367163.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            30265..31713
                     /gene="thiI"
                     /locus_tag="OQL90_000265"
     CDS             30265..31713
                     /gene="thiI"
                     /locus_tag="OQL90_000265"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1367164.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(31767..32357)
                     /gene="yajL"
                     /locus_tag="OQL90_000266"
     CDS             complement(31767..32357)
                     /gene="yajL"
                     /locus_tag="OQL90_000266"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1367165.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(32320..33231)
                     /gene="panE"
                     /locus_tag="OQL90_000267"
     CDS             complement(32320..33231)
                     /gene="panE"
                     /locus_tag="OQL90_000267"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1367166.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            33399..33890
                     /gene="yajQ"
                     /locus_tag="OQL90_000268"
     CDS             33399..33890
                     /gene="yajQ"
                     /locus_tag="OQL90_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1367167.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(34018..35382)
                     /locus_tag="OQL90_000269"
     CDS             complement(34018..35382)
                     /locus_tag="OQL90_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1367168.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(35531..36421)
                     /gene="cyoE"
                     /locus_tag="OQL90_000270"
     CDS             complement(35531..36421)
                     /gene="cyoE"
                     /locus_tag="OQL90_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1367169.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(36433..36762)
                     /locus_tag="OQL90_000271"
     CDS             complement(36433..36762)
                     /locus_tag="OQL90_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1367170.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIITILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(36762..37376)
                     /locus_tag="OQL90_000272"
     CDS             complement(36762..37376)
                     /locus_tag="OQL90_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1367171.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(37366..39357)
                     /gene="cyoB"
                     /locus_tag="OQL90_000273"
     CDS             complement(37366..39357)
                     /gene="cyoB"
                     /locus_tag="OQL90_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCS1367172.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(39379..40326)
                     /gene="cyoA"
                     /locus_tag="OQL90_000274"
     CDS             complement(39379..40326)
                     /gene="cyoA"
                     /locus_tag="OQL90_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCS1367173.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(40787..42262)
                     /gene="ampG"
                     /locus_tag="OQL90_000275"
     CDS             complement(40787..42262)
                     /gene="ampG"
                     /locus_tag="OQL90_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1367174.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(42306..42884)
                     /locus_tag="OQL90_000276"
     CDS             complement(42306..42884)
                     /locus_tag="OQL90_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1367175.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            43189..43506
                     /gene="bolA"
                     /locus_tag="OQL90_000277"
     CDS             43189..43506
                     /gene="bolA"
                     /locus_tag="OQL90_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1367176.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            43850..45148
                     /gene="tig"
                     /locus_tag="OQL90_000278"
     CDS             43850..45148
                     /gene="tig"
                     /locus_tag="OQL90_000278"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1367177.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            45393..46016
                     /gene="clpP"
                     /locus_tag="OQL90_000279"
     CDS             45393..46016
                     /gene="clpP"
                     /locus_tag="OQL90_000279"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1367178.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            46142..47416
                     /gene="clpX"
                     /locus_tag="OQL90_000280"
     CDS             46142..47416
                     /gene="clpX"
                     /locus_tag="OQL90_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1367179.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            47604..49958
                     /gene="lon"
                     /locus_tag="OQL90_000281"
     CDS             47604..49958
                     /gene="lon"
                     /locus_tag="OQL90_000281"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1367180.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            50167..50439
                     /gene="hupB"
                     /locus_tag="OQL90_000282"
     CDS             50167..50439
                     /gene="hupB"
                     /locus_tag="OQL90_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1367181.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            50631..52502
                     /gene="ppiD"
                     /locus_tag="OQL90_000283"
     CDS             50631..52502
                     /gene="ppiD"
                     /locus_tag="OQL90_000283"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1367182.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            52653..53024
                     /locus_tag="OQL90_000284"
     CDS             52653..53024
                     /locus_tag="OQL90_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1367183.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            53118..53516
                     /gene="fadM"
                     /locus_tag="OQL90_000285"
     CDS             53118..53516
                     /gene="fadM"
                     /locus_tag="OQL90_000285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1367184.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(53568..54263)
                     /gene="queC"
                     /locus_tag="OQL90_000286"
     CDS             complement(53568..54263)
                     /gene="queC"
                     /locus_tag="OQL90_000286"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1367185.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMEKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(54328..56028)
                     /locus_tag="OQL90_000287"
     CDS             complement(54328..56028)
                     /locus_tag="OQL90_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCS1367186.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLALG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            56128..56910
                     /gene="cof"
                     /locus_tag="OQL90_000288"
                     /pseudo
     CDS             56128..56910
                     /gene="cof"
                     /locus_tag="OQL90_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
     gene            57099..57557
                     /gene="decR"
                     /locus_tag="OQL90_000289"
     CDS             57099..57557
                     /gene="decR"
                     /locus_tag="OQL90_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1367187.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            57587..59359
                     /locus_tag="OQL90_000290"
     CDS             57587..59359
                     /locus_tag="OQL90_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1367188.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASENIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            59352..61133
                     /locus_tag="OQL90_000291"
     CDS             59352..61133
                     /locus_tag="OQL90_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1367189.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            61314..61652
                     /gene="glnK"
                     /locus_tag="OQL90_000292"
     CDS             61314..61652
                     /gene="glnK"
                     /locus_tag="OQL90_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1367190.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            61682..62968
                     /gene="amtB"
                     /locus_tag="OQL90_000293"
     CDS             61682..62968
                     /gene="amtB"
                     /locus_tag="OQL90_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1367191.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(63017..63877)
                     /gene="tesB"
                     /locus_tag="OQL90_000294"
     CDS             complement(63017..63877)
                     /gene="tesB"
                     /locus_tag="OQL90_000294"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1367192.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            64095..64667
                     /locus_tag="OQL90_000295"
     CDS             64095..64667
                     /locus_tag="OQL90_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1367193.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
BASE COUNT        15502 a        16195 c        17475 g        15518 t
ORIGIN      
        1 gcggcgaatg tgaaacgccg gtgcgtaagg cgaagcacgg tgattccagc ggcgtacgcg
       61 gcgctgcgtg gttatggcca caagagtaaa aaacgtaggc aattggcgca tcatgcctga
      121 tgcgacgctt gccgcgtctt atcaggcctg caaaatgtgc cagaaccgcg tagggcggat
      181 aaggcgttca cgccgcatcc ggcaataagt aatgaacacc gagactataa cctaccccag
      241 tggtttcgcc agcaagcacc ggagtatccg ccgcttgctg gcgcttatag cgaccataca
      301 gcagcaacga cgacatcgca gcaaacgaaa gcagggcggc aggcagcgcc acgtaattat
      361 atgccagccc cagcgtcagc atcataccac cgcaatatgc gccgacggcg ctaccgaggt
      421 taaacgctat ttgcccacct gcggcaccta ataactctcc gcctttggcg ttttgtagta
      481 acaatatttg tagcggtgct gaaagggcaa ataatcccgc gcaacaaata aaagcaaaaa
      541 taagcgacgt tgttttcatg ccgccgcaga aaaagaacat cagcagtgcc agtacaatta
      601 taaagtcagt cactgctgca atgcgcagtg gtgaataacg tcctgaaatc ctgccactta
      661 gcatatttcc cagcaccatc cctagcccaa ctaacatcat aataaaggtc atcgccgttt
      721 ccgaaaaacc ggaaataaac atcatgtatg gctttacgta gctgaaccag gcaaacacac
      781 ctgcgttgcc aaacatggtg gcggcgaaaa ttaaccacgg ggccgggctg cgcaaaaagt
      841 gaaattgttc gcgcagcttt cctttcgcct cgtcgcgaat atccggcacc caaaaataga
      901 ccgatgccat caccgcaata ttaaaaacag cgatcaataa aaaggtgtaa cgccagctaa
      961 attcctgact taaatacgtt cccagcggaa tgcccagcaa attggcgact gtcatcccgg
     1021 aaaccatccc cgccacggcg gcggtgactt ttccgggttt gataattttt gataacacga
     1081 tcgcgccgac gccaaaaaat gcgccatgcg gaaagccgga taccagccga ccaatggcga
     1141 gcatcaggta agacgaagag agcgtgaaca tggcgttgcc aatgacgcac aacgccacca
     1201 gaaacaacaa gatatgtttg agtgagtagc ggctggaaaa gagtgcgatg attggcgcac
     1261 cgaccaccac tcccagtgca taatacgaga tcatatgccc ggcagcagga atcgaaattc
     1321 ctacgttatg cgccagctcc gtgagcacgc ccataatgcc aaattcggcc atccccaaac
     1381 caaacgtgcc cagagccaaa gataaaatga cttttttcat accaccatca caaccaccag
     1441 aacggctacc aatcaccgta gccaataaag tgatagcttg caggataacg accagcaagg
     1501 ccaagagata attcgcccct ctgtattcat tatcctgctg aatagttatt tcactgcaaa
     1561 cgtactttcc agtttgctgt agcccaggcc gttgatcttt ttcactttga tctgcaccgg
     1621 aatacgctct ttcatcgctt ctacgtggct aatcacaccg atggttttgc cactggcgtt
     1681 cagggcatcc agcgcatcaa gggcggtatc cagcgtttcg ctatccagcg tgccaaaacc
     1741 ttcatcaagg aacagcgagt caatacgtgt tttatggctg accagatccg aaagcgccag
     1801 cgccagcgcc agactaacga ggaaactttc gccgccggaa agggtacggg tatcgcgtac
     1861 cgcatctgcc tgccaggtat caacaacctc gacttccagc gcctcgctgg ctttgcgctg
     1921 taacagatag cgcccgtgca gccgggtaag ttgctgatta gcgagatgga ctaaattatc
     1981 cagcgtcagc ccctgggcaa acttgcggaa tttatcgccc tctttggaac ctattagcga
     2041 attcagatat ccccagtcct caacctgctg cgtcatttga gcaatttgct gcattaaggt
     2101 ttgttgttgc tgacggttat ctgcatcctg cttcagctgc tggcgaatct cgccttgact
     2161 cgtggtgttt tcacgcaact tttggtgagt ttgcgctaac tcttgctgaa tctgctccac
     2221 cgtcacagtg agagccaacc cgtcaggtcg gtgttgttga tgctgtgcca gcgtttctgc
     2281 tgtctgagtg accagcgttt gcgcctgacg gcgctggttt tccagattct gcttgagctg
     2341 ttccagctgc gttagtgttt gttcatccat tagcgccgca aggaacgcct gctgatcgtc
     2401 aaagacgctg gcctgtagcg cggtgtcaaa ctgcgcctgg gctttttgca gactttgcgc
     2461 cgccagaaca tcctgttgct gtaacgtctg ctgctggctg tgtaatgcga gacattgttc
     2521 atgaacctgc cgccagttat ccagcgccac agtttcttcg ctgtgcggga gatcatcact
     2581 ttgcggcaac gtttccagaa tcggcgtcag ctgctgaata cggttttgca gcgtggttaa
     2641 ttcgttctgg cgttgctgcc agctctgcgc ttcttgctga cgtgtcgcca accagctctc
     2701 ttcttcatct tcctgtggca atgtcagtgc ataacccgcc aatgccgtta agagctgctg
     2761 ctggcgttgt tcaatttgct gttgatactg gataatttgc tgattatgcg cggcaatctg
     2821 cccttgtaat tcatgtcgtt ggctgagtaa ccgcagctgg cgtttgtgct catcttgtgc
     2881 atccagccac ggttgaatat cgtcctgtgg ctgcaaggtg atattgaggc tggccgtgac
     2941 ggcttgccat tgttgagtaa gtgcttgctc atcttgtcgg aggctttgcg cttcgttttc
     3001 atcgcgctga agctgcttcg ttaatgcatc cagttgcccg cgcagcgccg caccttcttc
     3061 gccgagcttt ttaacttcgt tttccagcgc cagtaatcga gactgattaa caccaggctc
     3121 cagcgcctga tacgccttga ccgccgggtg gctggtggaa ccacaaagtg gacaaggctg
     3181 acccgcctgt aactgtgcac gctgggcttc cagcgttttg atgcgcgctt cctgctcgca
     3241 aatggttttc acatcggcaa gttgctgcgt cttttcttta taacgctggc gcatttcgtt
     3301 aagtgcgaca ttacgttgcg tctgttccag agatccattc tggatagtga ccattaactg
     3361 cgccagacgt ttttgttggg gaacaatctg cccatgcagc gcgaccaggc gctgacgcag
     3421 tgggcgttgc tcagcatgtt gcgccagggc ggtagcaact tcatcggcgg ttaacgtcaa
     3481 cgtgatcgcc gcaagcgcat taagtttttg ctcagcatgg gttaactgtt gctgccattg
     3541 ccgcagatgc tcgcgatcgc tggtttgttg ggagaactgc gcacgccaac ccgccagttc
     3601 gttgttccac tgacggaagc ggtcgtgttc ctgtaaccag gtattcaggc tttgttgctg
     3661 ctgctgtaat tctgctgact gcttcgccgc gtggtggcga atgctcgcgc gaagcgccat
     3721 tgtgctctgt aagcgagtat ttacttcttc aatctgctgg cgagtatgcg ccagcgccgc
     3781 gctgtgttct gcgatgcgtt cccagtgcgg acgaagattt cgtgccggtt gtgccagact
     3841 gagcgccgcc agttgaggtt gcgctttttc ttcttcggct aacgcctgtt gcaaggcctg
     3901 ctgacggcgg ctggcttctt gctgcaattc gtccagacgc gttaaccagt ttagtgattg
     3961 ttgttcttgc tgctgcgcgg taagtaactg tttttcttcg tcagtaagta cctgcaaact
     4021 cgctgtcagc gattgcactt gttccggcgt gagcaacgcg acgccgctgg cctgcgcttg
     4081 cagcttctcc agctctgtgc gggccgattt gtgctgctca aaaaccatcg ccgagatttg
     4141 cccgtagatt tcagtgccgg ttaactcctc gagcaattcc gcgcgttctt tgggtttggc
     4201 attcaggaag gcagcaaatt gcccctgcga aagcagcatc gaacgggtga agcgcccgta
     4261 atccagcccg gttaacgtcg ctgtcagttc cagcttatct ttcactttgt cggcgagaat
     4321 tttgccgtcg gcgcagcgcg ccagctctac gcgtggcacc tgcaaattac cgtcgggttg
     4381 gttacgcgcc cgattctggc tccagaatgc acggtacgct tcacctttca cttcaaactc
     4441 cacctccgcc agacattcgg cggtatcgcg ggtcatgaga tcattttgcg attgtgaaac
     4501 gttagagaga cgcggagttt cgtgatacag cgccagacaa atggcgtcca gcagggtggt
     4561 tttccccgca ccggttgggc cggtaatggc aaacagcccg ttgctggcga acggctcgcg
     4621 ggtgaaatca atcttccatt cgccttttaa tgagttcagg tttttcaggc gcaggctgag
     4681 aattttcatg cttcgtgttc tccggcgagg gtatgcaacg tcgtggtgaa aagatgctgc
     4741 agacgttgct gctgcgattc atccagttct tccagtgcca gacggcgatt gaacacctct
     4801 tcgacgctga gttcgctgag ggtttcacgc tgttggctgg ctaacacacg ctcgcgctgt
     4861 tcacgactcc gacgtaccag caatacttcg acaggcaatg attcggctaa tgcctggatt
     4921 ttgcgctgaa tatcatgcag atactcatca gtagtgattt cgatatccag ccagacaggt
     4981 ggctcctgcg atacatcgcg ccactgttcc agctgtgcgg taatcgacgc cagatcgcct
     5041 ttcagcaccg ccatgggttg agttaccggt acgttcaggt tttccacgct ctctaatttg
     5101 ccgtttgaaa atgtcaccag atggacatat ttactcttac cgcattcatc aaaactcagt
     5161 ggaatggggg aaccgcaata gcgaacatgt tccatgccgc caataatctg tgcgcggtga
     5221 atatgcccga gcgcgatgta gtcggctggt ggaaagtttt gtgccggaaa cgcgtccagc
     5281 gtgccaatat aaatgtcacg cacggcgtca cttttactgg cacccacggt cgttaaatgt
     5341 cccgtggcga tgatgggcag aggctgatcg ccgcgcagtt tgcaggcatc ggcatagtgt
     5401 tgttggtaat aatcggtaat cgctgccagt aaatgctgct gtttttcaat accgttaagc
     5461 cccgcctggc tggaaataat gtcacgcggg cgtaaaaacg gaatggggca cagcactgtg
     5521 cctggcgtcc cgtcgcgacg aggcaagatt tgcggcgcat gtccggcgct ggcgaccacg
     5581 gttgtattga ggaacgccat gatatcgcgc gattcattca gcgtggcgac cgagtcatgg
     5641 tttcctgcca gtaccaccag atgacagcca gtttgctgta aattgacaac aaaacggttg
     5701 tataacgtgc gggcgtaact gggcggcgag ccggtatcga aaacatcacc agcaacaata
     5761 atcgcatcca cctgatgggt ttgtgctgtc tccagcagcc agtcaagaaa agcctgatgt
     5821 tcagcttcgc ggcttttact gtagaagttc tggccgagat gccagtctga ggtgtgaagg
     5881 atgcgcataa cggttccctg gcgaaaaagc atggtcgcga ttatacccaa acagatgtgc
     5941 catttgcttt tttctgcgcc acggaaatca ataacctgaa gatatgtgcg acgagctttt
     6001 cataaatctg tcataaatct gacgcataat gacgtcgcat taatgatcgc aacctattta
     6061 ttacaacagg gcaaatcatg gcgagacgta ttctggtcgt agaagatgaa gctccaattc
     6121 gcgaaatggt ctgcttcgtg ctcgaacaaa atggctttca gccggtcgaa gcggaagatt
     6181 atgacagtgc tgtgaatcaa ctgaatgaac cctggccgga tttaattctc cttgactgga
     6241 tgttacctgg cggctccggt atccagttca tcaaacacct caagcgcgag tcgatgaccc
     6301 gggatattcc agtggtgatg ttgaccgcca gaggggaaga agaagatcgc gtgcgcggcc
     6361 ttgaaaccgg cgcggatgac tatatcacca agccgttttc gccgaaggag ctggtggcgc
     6421 gaatcaaagc ggtaatgcgc cgtatttcgc caatggcggt ggaagaggtg attgagatgc
     6481 agggattaag tctcgacccg acatctcacc gagtgatggc gggcgaagag ccgctggaga
     6541 tggggccgac agaatttaaa ctgctgcact tttttatgac gcatcctgag cgcgtgtaca
     6601 gccgcgagca gctgttaaac cacgtctggg gaactaacgt gtatgtggaa gaccgcacgg
     6661 tcgatgtcca cattcgtcgc ctgcgtaaag cactggagcc cggcgggcat gaccgcatgg
     6721 tgcagaccgt gcgcggtaca ggatatcgtt tttcaacccg cttttaacgc cttgctcatc
     6781 ggacgcagag cagggcttat gatttcttaa ctggagtatc ttacgtgctg gaacggctgt
     6841 cgtggaaaag gctggtgctg gagctgctac tttgttgcct cccggctttc atcctgggtg
     6901 cattttttgg ttacctgccc tggtttttgc tggcatcggt aacaggactg cttatctggc
     6961 atttctggaa tttattgcgc ctttcatggt ggttgtgggt ggatcgcagt atgaccccgc
     7021 caccggggcg tggtagctgg gaaccgctac tatacggctt acaccagatg cagctgcgaa
     7081 ataaaaaacg ccgccgtgaa ctgggcaatc tgattaaacg ctttcgtagc ggcgcggagt
     7141 cgctgcccga cgcggtggtg ctgaccacgg aagagggcgg tattttctgg tgtaacggtc
     7201 tggcgcaaca aattcttggt ttgcgctggc cggaagataa cgggcagaac atccttaacc
     7261 tactgcgtta cccggagttt acgcaatatc tgaaaacgcg tgatttttct cgcccgctca
     7321 atctggtgct caacaccggg cggcatctgg aaattcgcgt catgccttat acccacaaac
     7381 agttgctgat ggtggcgcgt gatgtcacgc aaatgcatca actggaaggg gcgcggcgta
     7441 acttttttgc caacgtgagc catgagttac gtacgccatt gaccgtgtta cagggttacc
     7501 tggagatgat ggatgagcag ccgctggaag gcgcggtacg cgaaaaagcg ttgcacacca
     7561 tgcgcgagca gacccagcgg atggaagggc tggtgaagca gttgctaacg ctgtcgaaaa
     7621 ttgaagccgc gccgacgcaa ttgctcaatg aaaaggttga tgtgccgatg atgctgcgcg
     7681 ttgttgagcg cgaggctcag actctgagtc agaaaaaaca gacatttacc tttgagatag
     7741 ataacggcct caaagtgtct ggcaacgaag atcagctacg cagtgcgatt tcgaacctgg
     7801 tctataacgc cgtgaatcat actccagaag gcacgcatat caccgtacgc tggcagcgag
     7861 tgccgcacgg tgccgaattt agcgttgaag ataacggacc gggcattgca ccggagcata
     7921 ttccgcgcct gaccgagcgt ttttatcgcg ttgataaagc gcgttcccgg caaaccggcg
     7981 gtagcggatt agggttagcg atcgtgaaac atgctgtgaa tcatcacgaa agtcgcctga
     8041 atattgagag tacagtagga aaaggaacac gtttcagttt tgttatcccg gaacgtttaa
     8101 ttgccaaaaa cagcgattaa tccgcctttg tcatctttta ttgccataag ccagtcgatg
     8161 ctggcttatt ttctttgcag tcaaaatacg ggcgttagat tttacaacgg ttggtgattt
     8221 tttgttcgca tgattagcca tgtctttttc acggaaatag tgttttatac tggttggtga
     8281 tttcttatcg ctatatacct ctggttttta gatccctcct tgctttaaaa cgttataagc
     8341 gtttaaattg cgcttcaggt gctgtcatac tgactgcatt aacgcggtaa atcgaaaaac
     8401 tattcttcgc cgcgccaggt tgggagtatt tcccgctaaa attgtttaaa tataccgctg
     8461 tatcatcccc agggattggc acaaaaattt aacgttacaa caccacatcc acaggcagta
     8521 tgatttatga cccatcaatt aagatcgcgc gatatcatcg ctctgggctt tatgacattt
     8581 gcgttgttcg tcggcgcagg taacattatt ttccctccaa tggtcggctt gcaggcaggc
     8641 gaacacgtct ggactgcggc attcggcttc ctcattactg ccgttggcct gccggtatta
     8701 acggtagtgg cgctggcaaa agttggcggc ggtgttgaca gcctcagcac gccaatcggt
     8761 aaagtcgctg gcgtgctgct ggcaacggtt tgttacttgg cggtggggcc gcttttcgct
     8821 acgccgcgta cagctaccgt ttcctttgaa gtggggattg cgccgctgac gggtgattcc
     8881 gcgctgccgc tgtttatcta cagcctggtc tatttcgcta tcgttattct ggtttcgctc
     8941 tatccgggca agctgctgga taccgtgggc aacttccttg cgccgctgaa aattatcgcg
     9001 ctggtcatcc tgtctgttgc cgctattgtc tggccggcgg gttctatcag cacggcgact
     9061 gaggcttatc aaaacgctgc gttttctaac ggcttcgtta acggctatct gaccatggat
     9121 acgctgggcg caatggtgtt tggtatcgtt attgttaacg cggcgcgttc tcgtggcgtt
     9181 accgaagcgc gtctgctgac ccgttatacc gtctgggctg gcctgatggc gggtgttggt
     9241 ctgactctgc tgtacctggc gctgttccgt ctgggttcag acagcgcgtc gctggtcgat
     9301 cagtctgcaa acggcgctgc tattctgcat gcttacgttc agcacacatt tggcggcggc
     9361 ggtagcttcc tgctggcggc gttaatcttc atcgcctgcc tggtaacggc agttggcctg
     9421 acctgtgctt gtgcagaatt ctttgcccag tacgtaccgc tctcttatcg tacgctggtg
     9481 tttatcctcg gcggcttctc gatggtggtg tctaacctcg gcttaagcca gctgatccag
     9541 atctccgtac cggtgctgac cgctatttat ccgccgtgta tcgcactggt tgtattaagt
     9601 tttacacgct catggtggca taattcgtcc cgcgtgattg ccccgccgat gtttatcagc
     9661 ctgctttttg gtattctcga cgggatcaaa gcatctgcat tcagcgatat cttaccgtcc
     9721 tgggcgcagc gtttaccgct ggccgaacaa ggcctggcgt ggttaatgcc aacagtggtg
     9781 atggtggttc tggccattat ctgggatcgc gcggcaggtc gtcaggtgac ctccagcgct
     9841 cactaaatca ctgaacattt gttttaacca cggggctgcg atgccccgtg gttttttatt
     9901 gtgttgatgg gttaggaatt gatggaaagt aagaacaagc taaagcgtgg gctaagtacc
     9961 cgccacatac gctttatggc actgggttca gcaattggca ccgggctgtt ttacggttcg
    10021 gcagacgcca tcaaaatggc cggtccgagc gtgttgttgg cctatattat cggtggtatc
    10081 gcggcgtata tcattatgcg tgcgctgggg gaaatgtcgg tacataaccc ggccgccagc
    10141 tctttctcgc gttatgcgca ggaaaacctc ggcccgctgg caggttacat taccggctgg
    10201 acctactgct ttgaaatcct aattgtcgcc atcgccgatg tgaccgcttt tggtatctat
    10261 atgggtgtct ggttcccgac ggtgccgcac tggatttggg tactgagcgt ggtgctgatc
    10321 atttgcgccg taaacctgat gagcgtgaag gtattcggtg agctggaatt ctggttctcg
    10381 ttctttaaag tcgccaccat catcatcatg attgtcgccg gtttcggcat catcatctgg
    10441 gggattggca acggcgggca accgaccggt attcataacc tgtggagcaa cggcggcttc
    10501 ttcagtaacg gctggcttgg catggtaatg tcgttgcaaa tggtgatgtt tgcttacggt
    10561 gggatcgaaa ttatcgggat taccgccggt gaagcgaaag atcctgagaa atcgattccg
    10621 cgtgcgatta actccgtgcc gatgcgtatt ctggtgttct acgtcggtac gctgttcgtc
    10681 attatgtcta tctacccgtg gaatcaggtt ggcactgccg gtagcccgtt cgtgctgacg
    10741 ttccagcata tgggcattac ctttgccgcc agcattctta actttgttgt gctgaccgct
    10801 tcgctgtcgg caattaacag tgacgtattt ggcgtaggcc gtatgctcca cggtatggca
    10861 gagcagggca gcgcaccgaa aattttcagc aaaacgtcgc gtcgcggtat tccgtgggtt
    10921 acggtgctgg tgatgactac cgcgctgctg tttgcggtgt atctgaacta catcatgccg
    10981 gaaaacgtct tcctggtgat cgcttcgctg gcaaccttcg ccacggtatg ggtgtggatt
    11041 atgatcctgc tgtcgcaaat cgccttccgt cgccgtttgc cgccagaaga agttaaggcg
    11101 ctgaaattta aagtgccggg tggggtagca acgaccatcg gcgggctgat tttcctgctc
    11161 tttattatcg ggttgattgg ttatcacccg gatacgcgta tctcgctgta tgtcggtttc
    11221 gcgtggattg ttgtgctgtt gattggctgg atgtttaagc gtcgccacga tcgtcagctg
    11281 gctgaaaacc agtaatcctt gcgttctgta agccggttca ggaggaaatc ctgatccggc
    11341 tattccgaaa gttatccgcc cccgtcctcc tcccccaaat atccttcaga tgatgagtga
    11401 tcctgcatta ggctatggca aggtgatcag attttcatca caggggaatt atgatgttaa
    11461 atgcatggca cctgccggtg cccccatttg ttaaacaaag caaagatcaa ctgctcatta
    11521 cactgtggct gacgggcgaa gacccaccgc agcgcattat gctgcgtaca gaacacgata
    11581 acgaagaaat gtcagtacca atgcataagc agcgcagtca gccgcagcca ggcgtcaccg
    11641 cagggcttca gccgaatgcc gccggcacga ctggagcagt ttgccgtcga tgtaccggat
    11701 atcggcccac aatgggctgc ggatcagatt ttttatcaga tcttccctga tcgttttgcg
    11761 cgtagtcttc ctcgtgaagc tgaacaggat catgtctatt accatcatgc agccggacaa
    11821 gagatcatct tgcgtgactg ggatgaaccg gtcacggcgc aggcgggcgg atcaacgttc
    11881 tatggcggcg atctggacgg gataagcgaa aaactgccgt atctgaaaaa gcttggcgtg
    11941 acagcgctgt atctcaatcc ggtgtttaaa gctcccagcg tacatcaata cgataccgag
    12001 gattatcgcc atgtcgatcc gcagtttggc ggtgatgggg cgttgctgcg tttgcgacac
    12061 aatacgcagc agctgggaat gcggctggtg ctggacggcg tgtttaacca cagtggcgat
    12121 tcccatgcct ggtttgacag gcacaatcgt ggcacggggg gagcttgtta caaccctgaa
    12181 tcgccctggc gcgactggta ctcgtttagt gatgatggca cggcgctcga ctggcttggc
    12241 tatgccagct tgccgaagct ggattatcag tcggaaagtc tggtgaatga aatttatcgc
    12301 ggggaagaca gtattgtccg ccattggctg acagcgccgt ggagtatgga cggctggcgg
    12361 ctggatgtgg tgcatatgct gggggaggcg ggtggggcgc gcaataatat gcagcacgtt
    12421 gctgggatca ccgaagcggc gaaagaaacc cagccggaag cgtatattgt cggcgaacat
    12481 tttggcgatg cacggcaatg gttacaggcc gatgtggaag atgccgccat gaactatcgt
    12541 ggcttcacat tcccgttgtg gggatttctt gccaataccg atatctctta cgatccgcag
    12601 caaattgatg cccaaacctg tatggcctgg atggataatt accgcgcagg gctttctcat
    12661 caacaacaat tacgtatgtt taatcagctc gacagccacg atactgcgcg atttaaaacg
    12721 ctgctcggtc gggatattgc gcgcctgccg ctggcggtgg tctggctgtt cacctggcct
    12781 ggtgtaccgt gcatttatta cggtgatgaa gtgggactgg atggtaaaaa cgatccgttt
    12841 tgccgtaaac cgttcccctg gcaggtggaa aagcaggata cggcgttatt cgcgctgtac
    12901 cggcgaatga ttgtgctgcg taagaaaagt caggcgctgc gtcgtggcgg ctgtcaggtg
    12961 ctgtatgcgg aagataacgt agtggtattt gtccgcgtgc tgaatcagca acgtgtactg
    13021 gtggcaatca accgtggcga ggcctgtgaa gtggtgctac ccgcgtcacc gtttctcaat
    13081 gccgtgcaat ggcaatgcaa agaagggcat gggcaactga ctgacgggat tctggctttg
    13141 cctgccattt cggctacggt atggatgaac taacgtttta taacgccttg tgtgacgcct
    13201 gcgccatcat ccgcggataa aactgccaga agcgggttcc gagggcggta taatgggcgt
    13261 ttaaatcgta ccaggagtca cgcagggcat ccagacgtgg gcgacggctt gccatgccgt
    13321 ttaacacgtt ctggatgaaa tccatatcgc gatagcgcac cagccactgc cctgaccaca
    13381 agtaattgtt tagattgata aaacgtggcg gtgagtccgg caaaatcgtc atcacttgct
    13441 tgcgggcata acagataaat tcctgtagcg gaaagtccgg cgacagctgc gaccagtggc
    13501 gggaaagaaa gtgatcccac atgacatcca gcgtaatagg cgcaacgcgg cgcgtttcac
    13561 gacgaaacca ctcccgtgct tcgcggactt ccggcagatt gtcagtcaat acgtcgatac
    13621 gtcgatgcat atgaatgcca gccacgacgt cgggcggaaa actttcttca ggatttccgc
    13681 gtacgaaatc agccagtaaa ttgccggaaa gcgagctttc cgcgagatgg gctaaatgca
    13741 ggtgagctaa aaaattcatc gattatattc tatccaaagg ggggtaaagg ttgcagggag
    13801 agcgccccgg cactagacta cccgcctctt attttagtct gagtcagtgt catgcgcgtt
    13861 accgatttct tctttgaatt gcccgaatcc ctgattgccc actaccccat gcctgaacgc
    13921 agtagctgtc gtttactgtc gctggacggg cctacgggcg cgctgacgca cggtactttc
    13981 accgatttac tcgataagct caaccccggc gatcttctgg tttttaataa tactagcgtg
    14041 atcccggcgc gcctgtttgg gcgtaaagcc agcggcggca agattgaagt gctggttgag
    14101 cggatgctcg acgacaaacg cattcttgcg catattcgcg cctcgaaagc gccaaaacct
    14161 ggcgcagaac tgctgctggg cgatgacgaa agtattaacg caacaatgac cgcgcgccac
    14221 ggcgcactgt ttgaagtcga attcaatgat gatcgttcgg tactggatat cctcaacagt
    14281 atcgggcata tgccgctgcc gccgtatatc gaccgtccgg acgaagacgc tgaccgcgaa
    14341 ctttatcaaa ccgtttatag cgaaaaaccg ggcgcggttg cagccccgac cgctggtctg
    14401 cattttgacg agcctttgct ggaaaaattg cgcgccaaag gcgtggagat ggcgtttgtg
    14461 acgttgcacg ttggtgcggg caccttccag ccggtgcgcg tcgacaccat tgaagatcac
    14521 atcatgcact cggaatacgc tgaagtaccg caggatgtgg tagacgcggt actggcggcg
    14581 aaagcgcgcg gtaaccgggt gattgcggtt ggcaccactt cagtacgttc gctggaaagc
    14641 gcggctcagg cagcgaaaaa cgatctcatt gaaccgttct tcgacgatac ccaaatcttt
    14701 atctatccgg gcttccagta caaagtggtc gatgcgctgg tgacgaactt ccacttgcca
    14761 gagtcgacgc tgattatgct ggtttcggcc tttgccggtt atcaacacac catgaacgcc
    14821 tataaagcag cggtagaaga gaaatatcgc ttttttagtt acggtgatgc gatgtttatt
    14881 acgtacaatc cgcaggcaat taatgagcgc gtcggggagt aattccgcgg cgctggttta
    14941 aaacgttgga ctgtttttct gacgtagtgg agaaaaaatg aaatttgaac tggacaccac
    15001 cgacggtcgc gcacgccgtg gccgcctggt ctttgatcgt ggcgtagtgg aaacgccttg
    15061 ttttatgcct gttggcacct acggcaccgt aaaagggatg acgccggaag aagttgaagc
    15121 cactggcgcg caaattatcc tcggcaacac cttccacctg tggctgcgcc cgggccagga
    15181 aatcatgaaa ctgcacggcg atctgcacga ttttatgcag tggaaggggc cgatcctcac
    15241 cgactccggg ggcttccagg tcttcagcct tggcgatatt cgtaaaatca ccgaacaggg
    15301 cgtgcacttc cgtaacccga tcaacggcga tccgattttc ctcgatcctg aaaaatcaat
    15361 ggagattcag tacgatcttg gttcggatat cgtcatgatc tttgatgagt gtacgccgta
    15421 tcctgctgac tgggattacg caaaacgctc catggagatg tctctgcgtt gggcgaagcg
    15481 tagccgtgag cgttttgaca gtctcggaaa caaaaatgcg ctgtttggta tcattcaggg
    15541 cagcgtttac gaagatttac gtgatatttc tgttaaaggt ctggtagata tcggttttga
    15601 tggctacgct gtcggcggtc tggctgtggg tgagccgaaa gcagatatgc accgcattct
    15661 ggagcatgta tgcccgcaaa ttccggcaga caaaccgcgt tacctgatgg gcgttggtaa
    15721 accagaagac ctggttgaag gcgtacgtcg tggtatcgat atgtttgact gcgtaatgcc
    15781 aacccgcaac gcccgaaatg gtcatttgtt cgtgaccgat ggcgtggtga aaatccgcaa
    15841 tgcgaagtat aagagcgata ctggcccact cgatcctgag tgtgattgct acacctgtcg
    15901 caattattca cgcgcttact tgcatcatct tgaccgttgc aacgaaatat taggcgcgcg
    15961 actcaacacc attcataacc ttcgttacta ccagcgtttg atggcgggtt tacgcaaggc
    16021 tattgaagag ggtaaattag agagcttcgt aactgatttt taccagcgtc aggggcgaga
    16081 agtaccacct ttgaacgttg attaatatta ataatgaggg aaatttaatg agctttttta
    16141 tttctgatgc ggtagcggca acgggtgcac cggcgcaagg tagcccgatg tctttgattt
    16201 tgatgctggt ggtattcggt ctgattttct atttcatgat cctgcgtcca cagcagaagc
    16261 gcaccaaaga acacaaaaag ctgatggact ccattgccaa aggtgatgaa gttctgacga
    16321 acggtggcct ggttggtcgc gtaaccaaag tagcggaaaa cggctacatt gctatcgcgc
    16381 tgaatgacac cactgaagta gttattaaac gtgacttcgt agctgccgtc ctgccgaaag
    16441 gcaccatgaa ggcgctgtaa ttaaaatttt tccctaaggg aattgccgtg ttaaaccgtt
    16501 atcctttgtg gaagtacgtc atgctgatcg tggtgattgt catcggtctg ctgtatgcgc
    16561 ttcccaacct gtttggtgag gatccggctg ttcagatcac tggtgcgcgc ggagtcgccg
    16621 ccagtgagca aacgctgatc caggtccaga aaacgttaca agaagaaaaa ataactgcta
    16681 agtctgtggc actggaagag ggcgctattc ttgcgcgctt cgactccact gacacccagc
    16741 tgcgcgctcg tgaagcatta atgggcgtta tgggtgacaa atacgtcgtg gcgcttaacc
    16801 ttgccccggc aacgccgcgc tggctggcag ctattcacgc tgagccgatg aagctcggcc
    16861 ttgacctgcg tggcggcgtt cacttcctga tggaagtgga tatggacacc gcgcttggca
    16921 aactccagga acaaaatatc gatagcctgc gcagcgatct gcgtgaaaag ggcatcccgt
    16981 ataccaccgt tcgtaaagaa aacaactacg gcctgagcat tactttccgc gatgctaaag
    17041 ctcgtgatga agccattgcg tatctgagca agcgccatcc ggacctggtg attagtagcc
    17101 agggcagcaa ccagctgcgt gcggtaatga gcgatgctcg tctgagtgaa gcgcgtgaat
    17161 atgcggtgca gcagaacatc aatatcctgc gtaaccgtgt aaaccaactt ggcgtagcgg
    17221 agccggtggt tcagcgtcag ggtgctgacc gtatcgttgt tgaactgcca ggtattcagg
    17281 acactgcgcg tgcgaaagag attctgggtg cgacggcaac gctggaattc cgtctggtaa
    17341 acaccaacgt tgaccaggcc gctgcggcat ccggtcgcgt accgggcgac tctgaagtga
    17401 aacagacccg cgaaggtcag ccagttgtgc tgtacaaacg cgtaattctg accggtgacc
    17461 atatcaccga ctccacttcc agccaggacg aatacaacca accgcaggtt aacatctcgc
    17521 tcgatagcgc tggtggtaac atcatgtcta acttcactaa ggacaacatc ggcaaaccga
    17581 tggcaaccct gtttgtggag tacaaagaca gcggtaagaa agatgcaaat ggtcgtgcgg
    17641 ttctggtgaa acaggaagag gtgattaaca tcgccaacat ccagtctcgt ctgggtaaca
    17701 gcttccgtat caccggcatc aacaacccga acgaagcccg tcagctgtca ctgctgctgc
    17761 gtgccggtgc gttgatcgcg ccgattcaga ttgttgaaga acgtaccatt ggcccaaccc
    17821 tgggtatgca gaacattgaa caggggctgg aggcttgcct tgccggtctg ctggtttcta
    17881 ttctgttcat gatcatcttc tataagaagt ttggtctgat tgcgaccagt gctctgattg
    17941 ccaacttgat cttaatcgtc ggcattatgt cgctgttgcc aggcgcaacg ctgagtatgc
    18001 caggtatcgc gggtatcgtc ttaacccttg cggtggcggt cgatgcgaac gtactgatca
    18061 acgaacgtat taaagaagag ttgagcaacg gacgtactgt tcaacaggca attgatgaag
    18121 gttatcgtgg cgcatttagt tctatcttcg atgcgaacat caccacgctg attaaagtca
    18181 tcatcctgta cgcagtgggt accggggcaa ttaaagggtt cgcgattact accggtatcg
    18241 gtgtggcgac gtcgatgttt accgcgattg tcggtacgcg tgccatcgta aacctgctat
    18301 atggcggcaa gcgcgtcaag aagctgtcaa tctgaggagt gcgatgtggc acaggaatat
    18361 actgttgaac aactaaacca cggccgtaaa gtctatgact ttatgcgctg ggactactgg
    18421 gctttcggca tctctggtct gctgttaatc gccgctatcg ttattatggg cgtgcgcggc
    18481 tttaactggg ggctggattt caccggtggt acggttatcg aaattacgct cgaaaaaccg
    18541 gctgaaattg acgtaatgcg tgatgcattg caaaaagccg gttttgaaga gccaatgctg
    18601 caaaactttg gtagcagcca cgacatcatg gtccgtatgc cgcctgctga aggcgaaacc
    18661 ggcggtcagg tgctgggcag ccaggttctg aaggtgatta acgaatccac caatcagaat
    18721 gcagcagtga agcgtattga gttcgtcggt ccgagcgtgg gggaagacct tacgcaaacc
    18781 ggtgcgatgg cgttgatggc ggcgctgctg tctatccttg tgtacgtagg ttttcgcttt
    18841 gagtggcgac tggcggcagg ggttgttatt gcgctggcgc acgacgttat cattacgctg
    18901 ggtattttgt cgttattcca tatcgagatt gacctgacca ttgtggcatc gttgatgtcg
    18961 gttatcggtt actcgcttaa cgacagtatc gtggtatcgg accgtattcg tgaaaacttc
    19021 cgcaagatcc gtcgcggtac gccttacgaa atctttaacg tgtccttgac ccagacgctg
    19081 caccgtacct tgatcacatc cggtactacc ttgatggtta tcctgatgct gtacctcttc
    19141 ggtggtccgg tactggaagg cttctcgctg accatgctta tcggtgtttc catcggtact
    19201 gcatcttcca tctatgtggc atctgcgttg gctctgaaac tgggtatgaa gcgcgaacac
    19261 atgttgcagc agaaagtgga aaaagaaggg gcggatcagc cgtcaattct gccgtaatca
    19321 agttcccgtt gatgttgaaa atcccggtca gaagatcggg attttttttg atgtatggag
    19381 attgcgaaaa tccccgcatc ttgggaaact gcgcgtaacc ctacatttca tccaggtaac
    19441 tcttcatggc tatcatccca aaaaactatg cgcggttaga aagcggctat cgcgaaaaag
    19501 cattaaaaat atatccgtgg gtctgcggtc gctgttcccg cgagtttgtt tattccaacc
    19561 tgcgtgaact taccgttcac cacattgatc acgaccatac caataacccg gaagatggca
    19621 gtaactggga attgctttgt ctctattgcc acgatcatga gcattcgaaa tataccgaag
    19681 cggatcagta tggtacgacc gttatcgcag gggaagatgc gcagaaagat gtcggtgaag
    19741 cgaagtacaa cccattcgct gacctgaaag cgatgatgaa caagaagaag tgattaaaac
    19801 gtaaaattgc ctgatgcgct acgcttatca ggcctacgtt atttcagcaa tatattgaat
    19861 ttacgtgctt ttgtgggccg gacaaagcgt ttacgccgca tccggcatga acaaagctca
    19921 cgttgttaac aatcagaaat gccgggaata aatcccggca ttttcataat cagaagttgt
    19981 aacctactac caggtaacca ccccagccgg tagagcgaac gttgaagttg ccgttgccga
    20041 agttcagttc tgcatcgtcg ttccactgac caccgtcgtg ccagtaacga gctacgacag
    20101 agtagtgcca gtgatcgtag ttcagagcca gaatatggct ggaagcgata gagttattag
    20161 tacgggtctt aataccgttg attgcgttac cgctgtcatc ccctaaatcg gaaccccagt
    20221 cgaagttggt gaagccgatg tagctcagct gaccgcccca cagatcggta atcggcacaa
    20281 agtatttaat tttgaaacgg taaccgtccc actcgttttc gttcgctgcg ccatagttct
    20341 gccactggta tttcgcatag acgttcatgg acaggctcat cggcagatca gtatcgatgt
    20401 cggtacccag acccatgtac caggtgctct ggcgaccatt tttattacga cccatgtcgt
    20461 aaatgtagtt gttcgcgaag taccactctt tgaacggacc gaagttaagg tcagtattgg
    20521 tcagcttgtc gatggagaaa cgtggttcga tttccataaa cagcggagaa ccgtggttcc
    20581 agataccttt agcatcggag ttaccgccga agaataccgg cgcatccgca taaccataga
    20641 agtcgaacca gtctttttta gcgaatgctt cgtactcaag gtaggtatcg ttgcggatct
    20701 gcggtccgaa acgggtgtga tagcttccga caacgttaac gctctggtgc caccagtcgg
    20761 aaagatactg cggtttgtcg ttttcagctg cgttgacagt aaaagacgaa gagagcgcca
    20821 gtaccgcacc ggctgccagt aatgtttttt tcatatgtat gccactgttt gaaaatccct
    20881 tgcgggagtg aaaaaggcgc aaattgcgtt tctaaatatt tcgtatacag aggagcctat
    20941 tataaaaatc attgctcaca aaaatatgtt tcgtttcaca gttctatcat ttacgtaatc
    21001 gattgcgttc acgtttacac acattcgggc ggggattgta ctgactttcg cccttgttgc
    21061 aaacttttac tatcaatcca ggtgatgtaa gcagagtaac aaaatgacgg ggaaagatgt
    21121 tgcgggacgc acaaaatcgt cccgaaatac gtttactgca taacaggttg aatgtcgtgg
    21181 atacgtacaa agcctaactg atccacagaa atccccttta attgtaacgg aatatcaaca
    21241 tcgctggggg cgaggacgct ggcaggggca ttaatcaatt gattctgcac attgatttcc
    21301 tgaaagttgt ctattgttcc ctgaatttgc ccatattcaa cagttccgct gaatgcgggc
    21361 aagggatcat tcgactcacc ctgaatacgt agtgtaaggg tggtgccatc tgtatcaggc
    21421 gtaatattca ccagcgacat acgtaaagta ccgatctggc tttccagtct tgccggtgtt
    21481 ttcgctctag gcagaaggta acccccactg ctggattttg cattcagcct gttttgctga
    21541 gtgattttga ctgtttcttg attgagttgc gtcatctcct ggttcagcgt gctgacgctg
    21601 tgtttcatct gtcgcacttc actttgctgc acacaggcgc taaggctgaa caggcttccc
    21661 agcaggagca gtcgaaaaac gttagtattc ataggtcgtc cctttctcgt cgcaattagc
    21721 ctaatggtag agggtaaaag cagcgaaagc atcgatcctt tatctcaaaa gcgttgcgcc
    21781 tttgttgtat cgtcagttca gggtaaaata gatttccgtt aaccacttgg tcaggacgcc
    21841 gtatgcattg cccattctgt ttcgccgtgg acactaaggt aattgactct cgtctcgtgg
    21901 gcgagggttc atccgtacgc cgccgtcggc agtgtctggt gtgtaatgaa cgtttcacca
    21961 cctttgaagt ggcggagctg gttatgccgc gtgttgtaaa aagcaacgac gtgcgtgaac
    22021 cgtttaatga agagaaattg cgtagcggaa tgctgcgggc gctggaaaaa cgtccggtga
    22081 gttccgatga cgttgaaatg gcaatcaatc atattaaatc gcagctgcgc gccaccggtg
    22141 agcgcgaagt accgagcaag atgattggca atctggtgat ggagcaattg aaaaagctcg
    22201 ataaagtcgc ctatatccgt tttgcctctg tctaccgcag tttcgaagat atcaaagaat
    22261 ttggcgaaga gatcgcgcgc ctggaggact aagccgtgca ggacgagtat tacatggcgc
    22321 gggcgctaaa gctggcgcaa cgaggacgtt ttaccaccca ccccaaccca aatgtcgggt
    22381 gcgtcattgt caaagatggc gaaattgtcg gtgaaggtta ccatcaacga gcgggtgaac
    22441 cacatgccga agtacacgcg ttgcgtatgg cgggtgaaaa agccaaaggt gcaacggcgt
    22501 atgtgacact cgaaccttgc agccatcacg gccgtacgcc gccttgctgt gatgctttaa
    22561 ttgccgcagg tgtggcgcgc gtggttgcct cgatgcaaga tcctaacccg caggtcgctg
    22621 ggcgtggact ttaccgtctg caacaggctg gcattgacgt cagccacggc ctgatgatga
    22681 gtgaagccga gcaattgaat aaaggctttc tcaagcggat gcgcaccggc tttccttata
    22741 ttcagttaaa acttggcgca tcgcttgatg gtcgcacggc gatggcgagc ggcgaaagcc
    22801 agtggatcac ttcgccccag gcgcggcgtg atgtacaacg actgcgcgcg caaagtcatg
    22861 ccattttaac cagcagcgcc acggtgctgg cggatgatcc tgccttaacg gtgcgttggt
    22921 ccgaactgga tgaacaaact caggcgctct acccgcaaca aaatctccgc cagccggtac
    22981 gtattgtgat tgatagccaa aatcgcgtga cgccggaaca tcacattgtg cagcagcccg
    23041 gcgaaacctg gttcgcgcgt acgcaggaag attctcgtga gtggccggaa acggtgcgta
    23101 ccttgctgat tccagagcat aaaggtcatc tggatctggt tgtactgatg atgcaactgg
    23161 gtaaacagca gattaacagc atctgggggg aagcggggcc aacgctcgct ggcgcattgc
    23221 tgcaggcggg tttagtcgat gagctgattg tctatatcgc acctaaacta ttaggcagcg
    23281 acgcccgtgg attatgcacc ctgccagggc ttgagaaatt agccgacgcc ccccaattta
    23341 aattcaaaga gatacgtcat gtaggcccgg atgtttgcct gcatttagtg ggtgcatgat
    23401 ctcccggctc gaaagggaag cagcgcacga aatattatgc taaaatccgc ccccctgcgg
    23461 ggccatactc gaacccgaag gaagaaaatg aacattattg aagctaacgt tgctaccccg
    23521 gacgctcgcg tcgccatcac catcgcgcgt ttcaacaact ttatcaatga cagcctgctg
    23581 gaaggtgcaa ttgacgcact gaaacgtatc ggtcaggtaa aagatgaaaa cattaccgtt
    23641 gtttgggtgc ctggtgccta tgagctgccg ctggcggcgg gtgcactggc taaaaccggt
    23701 aaatacgacg cggtgattgc gctgggtacg gttattcgtg gtggcactgc ccattttgaa
    23761 tatgtcgctg gtggtgcaag caacggcctg gcgcatgttg cccaggacag cgaaattccg
    23821 gttgcttttg gggttctgac cactgaaagc attgaacaag cgatcgaacg tgctggcacc
    23881 aaagctggca acaaaggtgc agaagctgca ctgaccgcgc ttgaaatgat taatgtattg
    23941 aaagccatca aggcctgaaa ttagtaaggg gaaatccgtg aaacctgctg ctcgtcgccg
    24001 cgctcgtgag tgtgccgtcc aggcgctcta ctcctggcag ttgtcccaga acgacatcgc
    24061 tgatgttgaa taccagttcc tggctgaaca ggatgtaaaa gacgttgacg tcctgtactt
    24121 ccgtgagctg ctggccgggg tggcgactaa taccgcatac ctcgacggac tgatgaagcc
    24181 atacctgtcc cgtctgctgg aagaactggg acaggtagaa aaagcagtac tgcgcattgc
    24241 gctgtacgaa ctgtctaaac gtagcgatgt gccatacaaa gtggccatta acgaagcgat
    24301 cgaactggcg aaatcgttcg gcgcagaaga cagccataag ttcgtcaacg gtgtactcga
    24361 taaagcagca cctgtgattc gccctaacaa aaagtaatat ccaggccggt agattcacgg
    24421 aagaccgttc catgatcgcc ggccttttct tttttacctg ctgaggcata acgtatggca
    24481 tgtggcgagt tctccctgat tgcccgttat tttgaccgtg taagaagttc tcgtcttgat
    24541 gtcgaactgg gcatcggcga cgattgcgca cttctcaata tccccgagaa acagaccctg
    24601 gcgatcagca ctgatacgct ggtggcgggc aaccacttcc tccctgatat cgatcctgct
    24661 gatctggcgt ataaagcact ggcggtgaac ctaagcgatc tggcagcgat gggggccgat
    24721 ccggcctggc tgacgctggc attaaccttg ccggacgtag acgaagcgtg gcttgagtcc
    24781 ttcagcgaca gtttgtttga tcttctcaat tattacgata tgcaactcat tggcggcgat
    24841 accacgcgtg ggccattatc aatgacgttg ggtatccacg gctttgttcc gatgggacga
    24901 gccttaacgc gctctggggc gaaaccgggt gactggatct atgtgaccgg tacaccgggc
    24961 gatagcgccg ccgggctggc gattttgcaa aaccgtttgc aggttgccga tgctaaagat
    25021 gcggactact tgatcaaacg tcatctccgt ccatcgccgc gtattttaca ggggcaggca
    25081 ctgcgcgatc tggcaaattc agctatcgat ctctctgacg gtctgatttc cgatctcggg
    25141 catatcgtga aagccagcga ctgcggcgta cgtattgacc tggcattgct gccgttttct
    25201 gatgcgcttt ctcgccatgt tgaaccggaa caggcgctgc gctgggcgct ctctggcggt
    25261 gaagattacg agttgtgttt cacggtgccg gaactgaacc gtggagcgct ggatgtggct
    25321 ctcggacacc tgggcgtacc gtttacctgt atcgggcaaa tgaccgccga tatcgaaggg
    25381 ctttgtttta ttcgtgacgg tgaacctgtt acgttagact ggaaaggata tgaccatttt
    25441 gccacgccat aaagatgtcg cgaaaagtcg cctgaagatg agtaatccgt ggcatctact
    25501 tgctgtcgga ttcggaagtg gattaagccc gatcgttcct gggacgatgg gctcgctggc
    25561 agcgattccg ttctggtatc tgatgacctt tttgccctgg cagctctact cgctggtggt
    25621 gatgctgggg atctgtatcg gcgtctatct ttgtcatcag accgcaaaag atatgggcgt
    25681 gcacgatcac ggcagcattg tctgggacga atttattggt atgtggatca cgctcatggc
    25741 gctgccgacc aatgactggc agtgggttgc cgccgggttt gtgattttcc gtattctgga
    25801 tatgtggaaa ccgtggccga tccgctggtt tgatcgcaat gtgcatggcg gcatggggat
    25861 catgatagat gatattgtcg ccggggtgat ctccgctggc atcctgtact ttattggtca
    25921 tcactggccg ctgggcattc tgtcgtagtt gtgcaccgat gcctgatgtg acgcttgttg
    25981 cgtctcatca ggcctggact cttatttaaa tcctactacc ggatgcggtt tatacggcgt
    26041 ttccagttcg gcaatctgtt ccggcttcaa agtgatatcc accgcgttca atagctcatc
    26101 aagctgttct tcccgcgatg taccgataat cggtgcggca atgcccggtt tactcaacaa
    26161 ccaagccagc gcaacttgtg ctcgtgttgc accgagttct tcactgacgc ccgttaaccg
    26221 ctctgcgatc tgcgcgtcat tttcatcgct ttctttatag agatttttcc ccacctcatc
    26281 agacaccagt cgtgcggtag tttctcccca cggacgcgtc agtcgccccc gcgccagcgg
    26341 gctccacgga atcaccgcca cgccctcctg ataacacagt ggcagcatct cgcgctcttc
    26401 ttcgcgataa atcagattgt agtgatcctg catactgaca aactgcgccc agccgtgctg
    26461 tttttggagt tccagtgcct gagcaaactg cgaagcgtgc attgatgacg cgccgatata
    26521 acgcgctttc ccggctttta ccacgtcgtt cagggcttcc agcgtctctt cgatcggcgt
    26581 gttgtaatcc cagcgatgaa tttgcaggat atcgacataa tccatgccga gacgtcgcag
    26641 gctgtcgtcg atagagcgca aaatttgtgc acgggataat ccttccggta aatcaccaac
    26701 gcgatggaac actttggtgg caacgaccac gtcttcacga cgggcgaaat cccgcagtgc
    26761 gcgaccaacg atctcttcgc tgctaccatc ggaatagcta ttggcggtat caaagaaatt
    26821 tatgccgcct tccagtgcgc gtttaattat gggacggctg ctctcttccg gcagtgtcca
    26881 tgcgtgatta ccacgatctg gctcgccaaa ggtcatacag ccgaggcaaa gtcgggaaac
    26941 gcgaaggtcg gtttttccta aggggttgta ttgcatgctg ccactcctgc catgaataat
    27001 ttcttaagca tagcaggagt ggagtaggga ttatgccagc caggccttga ttttggcttc
    27061 cataccggcg gcatcgaggc cgagttcggc gcgcatttct tcctgcgttc cttgcggaat
    27121 aaagaagtca ggcaggccaa tgttcagcac ggggactggt ttacgatggg ccattagcac
    27181 ttcgttcact ccgctgcctg caccgcccat aatggcgttt tcttctacgg taaccagtgc
    27241 ttcatgactg gcggccattt ccagaattaa cgtttcatca agcggtttca caaaacgcat
    27301 atcgaccagc gtggcgttca gcgattcggc gactttcgcc gcttctggca tcagcgtacc
    27361 aaagttaagg atcgccagtt tctcgccacg acgcttcaca atgcctttgc caattggtag
    27421 tttttccagc ggcgtcagtt ccacgccgac cgcgttgcca cgcgggtagc gcaccgctga
    27481 cgggccatcg ttatagtgat agccggtata gagcatctgg cgacattcgt tttcatcgct
    27541 cggggtcata atgaccattt ccggtatgca gcgcaggtaa gagagatcaa aagcaccctg
    27601 atgggtttga ccgtcagcac caacaatgcc cgcgcggtcg atggcgaaca ggaccggaag
    27661 cttttgaatt gccacgtcat gcagcacctg atcataggcg cgttgcagga aagtggagta
    27721 gatcgcgaca atgggtttgt acccaccaat cgccagaccg gcggcaaagg tcactgcgtg
    27781 ttgctcggca attgccacgt cgaagtagcg atccgggaat ttacgagaaa actcgaccat
    27841 gccggaacct tcacgcatcg ccggagtaat cgccatcagc ttgttatctt tcgccgccgt
    27901 ttcgcacaac cagtcgccaa agatttttga atagctcggt aaaccgccgc tacttttcgg
    27961 caaacaaccg ctggagggat caaatttagg cacggcgtgg aaagtgattg ggtctttttc
    28021 tgccggttca taaccacgac cttttttggt catgatatgc aggaactgcg ggcctttcag
    28081 gtcacgcatg ttcttcagcg tggtgataag ccccagcaca tcgtgaccgt caaccgggcc
    28141 gatgtagtta aagcccagct cttcaaacaa cgtgccaggg actaccatgc ctttaatatg
    28201 ttcttcggta cgtttgagca gctctttaat gggtggcacg ccagagaaaa cttttttccc
    28261 gccttcgcgc agtgaagagt aaagcttacc ggaaagcagc tgcgccagat ggttgttgag
    28321 cgcgccgaca ttttcggaaa tcgacatttc gttgtcgttg aggaccacca gcatatcagg
    28381 acggatatcg cccgcgtgat tcatcgcttc aaacgccatg cctgcggtaa tcgcgccatc
    28441 gccgattaca cagacggtac ggcgattttt gccttctttc tcggcagcaa ccgcaatacc
    28501 aattccggca ctgatggagg ttgatgaatg cccgacgctt aatacgtcat attcgctttc
    28561 gccgcgccac gggaacgggt gcaggccgcc tttctgacgg atggtgccga ttttgtcgcg
    28621 gcgtccggtc aaaattttat gcggataagc ctgatgcccc acatcccaaa tcaattggtc
    28681 aaacggggtg ttgtagacat agtgcagcgc cacggtcagt tcgaccgtgc ccagcccgga
    28741 ggcgaagtgc ccgctggaac ggctcacgct gtcgagtaaa tagcggcgca gttcgtcgca
    28801 gagtttcggt aaactctctt tcggcaacag tcgtaactcc tgggtggagt cgaccagtgc
    28861 cagggtcggg tatttggcaa tatcaaaact catcaggggc ctattaatac ttattgttta
    28921 tttattacgc tggatgatgt agtccgctag cgcttccagt gccgaggtat cgggtgactg
    28981 ttcagccagt tgtttcagcg actgacgggc atcgtcgatc agatcccggg ctttcttccg
    29041 ggcttgctca agacccagaa gtgcagggta ggtactttta ccaagttgct ggtcggcacc
    29101 ctggcgtttt cccaacgttg cagtatctcc taccacatcc aggatgtcat cctgaacctg
    29161 gaaggcaagg ccgatgctct ctgcgtactt gtcgagtact ggcagagcac gacgcccttt
    29221 atctccggcg cttaatgcac caaggcgaac ggcggcgcga atcaatgcgc cggttttatg
    29281 acgatgaata cgctcaagcg cgtccagagg tacgtgtttg ccttccgcgt ctaaatctaa
    29341 tgcctgacca ccgcacattc cggcaatacc gctggcgctc gccagttcag aaatcatcga
    29401 aattctgtcg cgatccgaca cttccggcat atcagcatcg cttaaaatcg agaacgccag
    29461 cgtttgtaaa gcgtcgccag cgagaatcgc gtttgcttcg ccaaacttca catggcaggt
    29521 cggcaaaccg cgacgcagat cgtcatcatc catcgccggt aaatcatcat gaattaatga
    29581 gtaagcgtgg atacactcta cggcggcagc gggtgcgtcc agcgtgtttg tgctaacgcc
    29641 aaacatatga ccggtggcat aaaccaggaa gggtcgcaga cgcttaccac ctaataatgc
    29701 accatactgc atggtttcga ccacgggagt gttctgaaag ggcagtgggg cgataaaacg
    29761 gctcagcgcc tggttggcct gcttaacgca ggcttcgagt tgctgcggaa agtccattac
    29821 tcattgtccg gtgtaaaagg ggttagagag gcgtcttcat tgtcagacag cagaatttgt
    29881 acgcgctgtt cggcttgttg taatttggcc tgcccctgac gtgccagctg cacgccgcgt
    29941 tcgaactcgt tcagcgcctc ttccagcggc aggtcgccac tttccagacg ggttacaatc
    30001 tgttccagct cgctcagcgc cttttcaaag ctggcgggag cctcattttt cttcggcata
    30061 atgaatgtct gactctcaat attttttgcc ccgtcatggt aacggactca gggcaaatag
    30121 caaataacgc gcaatggtaa ggtgatgtgc gcagcaaagc gatgttagtg gtatacttcc
    30181 gcgcctggat gcagccgcag gtgtgggctg ctgtattttt ccctatacaa gtcgcttaag
    30241 gcttgccaac gaaccattgc cgccatgaag tttatcatta aattgttccc ggaaatcacc
    30301 atcaaaagcc aatctgtgcg cttgcgcttt ataaaaatcc ttaccgggaa cattcgtaac
    30361 gttttaaagc actatgatga gacgctcgct gtcgtccgcc actgggataa catcgaagtt
    30421 cgtgcaaaag atgaaaacca gcgtctggct attcgcgacg ccctgacccg tattccgggt
    30481 atccaccata ttctcgaagt cgaagacgtg ccgtttaccg acatgcacga tatttttgag
    30541 aaagcgttgg ttcagtatcg cgatcagctg gaaggcaaaa ccttctgcgt acgcgtgaag
    30601 cgccgtggca aacatgattt tagctcgatt gatgtggaac gttacgtcgg cggcggttta
    30661 aatcagcata ttgagtccgc gcgcgtgaag ctgaccaatc cggaagtgac tgtccatctg
    30721 gaagtggaag acgatcgtct tctgctgatt aaaggccgct acgaaggtat tggcggtttc
    30781 ccgatcggca cccaggaaga tgtgctgtcg ctcatttccg gtggtttcga ctccggtgtt
    30841 tccagttata tgttgatgcg tcgcggctgc cgcgtgcatt actgtttctt taaccttggc
    30901 ggcgcggcgc atgaaattgg cgtgcgtcag gtggcgcatt atctgtggaa ccgttttggc
    30961 agctcccacc gcgtgcgttt tgtcgctatt aatttcgaac cggtcgtcgg ggaaattctc
    31021 gagaaaatcg acgacggtca gatgggcgtt atcctcaaac gtatgatggt gcgtgccgcg
    31081 tctaaagtgg ctgaacgtta cggcgtacag gcgctggtca ccggcgaagc gctcggccag
    31141 gtgtccagtc agacgctgac caacctgcgc ctgattgata acgtctccga tacgctgatc
    31201 ctgcgtccgc tgatttctta cgacaaagag cacatcatca acctagctcg ccagattggc
    31261 accgaagact ttgctcgcac gatgccggaa tattgcggtg ttatctccaa aagcccgacg
    31321 gtgaaagcgg ttaagtcgaa gattgaagcg gaagaagaga aattcgactt cagcattctc
    31381 gataaagtgg ttgaagaagc gaataacgtt gatatccgcg aaatcgccct gcagaccgag
    31441 caggaagtgg tggaagtgga aaccgtcaat ggcttcggcc cgaacgacgt gatcctcgat
    31501 atccgttcta tcgatgaaca ggaagataag ccactgaaag tcgaagggat tgatgtagtt
    31561 tctctgccgt tctataaact gagcaccaag tttggcgatc tcgaccagaa cagaacctgg
    31621 ctactgtggt gtgagcgcgg ggtgatgagc cgtctgcagg cgctctatct gcgcgagcag
    31681 ggctttaaca atgtgaaggt atatcgcccg taatttgtgg ttgtaacgtc gcatctggtc
    31741 agatgcgacg cttaccgcat ccgacactac tcgtaataat tataaatccc tgccgccatc
    31801 accagttgtg atgccacttc atgggctttc tcgcgcccaa cctgcaggtc gataattttc
    31861 agaccaaagt cgatagctgt ccccggcccc tggctggtca gcaattttac ccgtgcatcc
    31921 cagacgacgc gcttgtcctg ccattgttca gcgggaattt tgtctttcag cgtcgggaag
    31981 ccggtcatat taccaatcgg gaagatatcg tgcggcacca gcacggtggc tggcgcggcg
    32041 caaatagccg cgacgatacg cccggaacgg tggaactgtt taacggtttc aaccagcaga
    32101 gtgctatcgc gaaaacactc tgcgccttta atgccaccag gcagtacgat cacgtcatat
    32161 tcgccatcag ccacctcgac cagcggcgca tccgccagca gcttcacgcc gcgcgagcag
    32221 gtaattgcca ggttgccatc gctggctacg ctggcagtgg tgactttgat accgccgcga
    32281 accaagaggt cgatcgtggt gacggcttca gtctcttcac taccaggggc gaggcaaacc
    32341 agtgccgatg cgctcatatt cactctcctt tctttttacc atttcaaaca ggcgggtgtt
    32401 ttccggtacg gcaatcccat gcgcgcgggc gcggcgtaag agaaaaccat tgatatagtc
    32461 gatttcggtg tggcgcagcg cgcggatatc ctgcaacatc gacgagatat tttccgctgt
    32521 ggcatcaatc acctgcatca cgtaatcacg caaatcttcc gctgaagtat gatgcccttc
    32581 gcgttcgatc actgccgcga cttcttcgca tatttgcata atttcttgcg gatgatgacg
    32641 taattcaccg ttcgggcaat tccagatggc ggtcagtgga ttaatcacgc agttgactgc
    32701 cagcttgcgc cacagctcgg cgcgaatatt gttatgccag gcaacgtcag gcaacacggt
    32761 ttgtaaaata tccgccagat aactgtaatc gccatcctgt tgccgtgccg gaccaatatg
    32821 cgtgataccg tttgccacat gaataatgac attgccgtcg cggcgggcgg catgggtggt
    32881 ggtgcccatc agtaatggct gctgaatatt ttgcaactct tcgatggtgc ccatgccgtt
    32941 gtgaattaac agtattggcg tggttacagg cagtgtggac gcgaggcttt tgacggcatc
    33001 ggaaacctgc catgctttca gcgtcaccag gagcagatcg ctggtggcga gaaaatcggg
    33061 atcgttggcg gtcagcgatt cgttaaatat cgaaccatct gtctcaacca gattcgcact
    33121 acaataaggt tgcggtacgc gcagccagcc ctgaacttca tgaccctgtt tgcaaagtgc
    33181 tgtaagccat aattgcccta aggcaccgca tcccaatacg gtaattttca ttgttcctcc
    33241 tcacccgcaa ccactccggg tgttcaataa ggctatccct taattgtgca tgctgttgcg
    33301 actatgcaca attaagggat acgtcctggt gcaggactgt cggttattta actttgcggg
    33361 tattatgctt cgcatcaaaa atgaagggag aggaaaagat gccatctttc gatattgtct
    33421 ctgaagttga tcttcaggaa gcacgtaacg cggtcgataa cgcgagccgc gaagtggagt
    33481 cccgttttga cttccgtaac gttgaagcct catttgagct gaacgacgcc agcaaaacca
    33541 tcaaagtgtt gagcgagtcc gatttccagg tcaatcagtt gctggatatt ctgcgtgcca
    33601 agctgctgaa gcgcggcatt gaaggcagtt cactggatgt accggaaaat atcgttcata
    33661 gcggtaaaac ctggtttgtg gaagcgaaac tgaagcaggg cattgagagc gcgacccaga
    33721 aaaaaatcgt caagatgatc aaagacagca aactgaaagt gcaggcgcaa attcagggcg
    33781 atgagatccg cgtgactggc aaatctcgtg atgatttgca ggctgtcatg gcgatggtgc
    33841 gcggtggcga tctgggtcag ccgttccagt tcaaaaactt ccgcgattaa tcgcgacaac
    33901 ctgcgctttg ttcatgtcag atgcggcatg aacgcctggt tcggcctaca aaggcttgta
    33961 aatcaggagc ttagtaggcc tgatgagcgt agcgcagcag gcaatttcgc ctcgctttta
    34021 tgcctggcga attgcctgtt caacatcaaa gcgattcgtc actttgctgt cgattttcac
    34081 ataagctgaa tgttcttctt ctgcaatcaa cacttctttg atgccttcag tttctagcaa
    34141 acgcactttt aacgcctcgt ttgcggcaat gttcgccggg atttcaatgc gcaaactgct
    34201 gacatacgcc ggttctttca tggtactggc gactgccagc cacactgcgg ccagcattgc
    34261 gccagcgaga aatacgccct gaccgtcaaa catgccgtca atccagccgc ccagtgaacc
    34321 gccaatcgcc acgccaagaa actggctggt ggagtaaaca cccatcgctg tacctttgta
    34381 acctgctggc gactctttac tgataagtga aggcagaagg gcttccatca aattaaacgc
    34441 tacaaagaaa agctgcacgc cgaccaccag ttgccagaac tgcgtttgcg cgttccacaa
    34501 cacaatttcc gcaaccacga tcaatccgac gcagaagaca aagacttgct tcattttgcg
    34561 cttaacttca gcgtagataa tgaaaggcac gaccgagcca aaggcgatta gcattgtcgc
    34621 cagatagacc ttccagtgtt cagccgccgg gaaccctgca tcagccagtt gtccgggcag
    34681 ggcaacaaac gtcgacatca gcaaaatatg cagacacata atgccaaagt tgagtttcag
    34741 cagccgcggt tccgccagca ctttactgaa actgcctttc accattccgg actcacgatt
    34801 aagtacgtga gtgctactgt tgggcacaac ccaaatggtc aacgcaatgc cggtcgttgc
    34861 cagaatagcg atcatccaga acagcgcgtg cagcccaagt ttgtgagtga tgatcgggcc
    34921 aagcaccatc gcaatggcaa aggtaatgcc aaagctcacg ccgataaacg ccattgcttt
    34981 ggtgcggttt tgttcgcgcg tgagatcgga aagcagcgcc ataacggcag cggcaatcgc
    35041 accggagcct tgtagcgccc ggcctagaat aattccccag atggagtcag agagcgcagc
    35101 gataacgcta ccggcggcaa acaccgccag cccaccgaca attaatggtt tgcgaccaat
    35161 acggtcggaa agcaggccaa acggaatctg aaaaacggcc tgagtcagac cataaatacc
    35221 aatggcaata ccgattaatg cttcgctggc accttgcaga gccatgccat acgtggtcag
    35281 aaccggcaga accataaaca tgcccagcat gcgcaacgag aatacggtcc ctaaacccca
    35341 ggtcgcgcgc ctctcacctg gcgtcatttt ataatcgttc attaccacct ctgttttaaa
    35401 ttcgcgacta gtgtaaagcg gcaaacgaat ggggtaaaca tgcggatttt ttagtaaata
    35461 tttcgagagg gatatgtttc taatgctaag aaaaaaggtg ccgtagcacc tttttaatag
    35521 agaggttttg ttaccacaca gcagccagca gcgtatgcga gtccggtacc ataaaatcaa
    35581 cggacatcat caccgagagg gcagtgatgg cgatgataga gaagccgaac agcttgcgcg
    35641 cccagattct gtcatcagca actttataac cgcgcagagc catacctaac caccagacgc
    35701 taaccgccgc ggcgaccacc agatatttat atccagcgta accgccaaga gagagcatca
    35761 gcgtggcaac ggcaaaggcg atgatataca gcgtgatgtg attcttcgcc accgaaatgc
    35821 cttttaccac tggcaatacc ggaatgtttg ccgcctggta atccttaaag cggaaaatgg
    35881 cgatggcata ggagtgaggc atctgccaca ggctgaagat agccagcagg atcgctgcgc
    35941 cgctatcgaa ctcgccggtt actgcacagt agccaatcac cggcggcgca gcgccggaga
    36001 gcgaaccaat caatgtgccg tagactgagt ggcgtttcat gtacaggcta tagacgccga
    36061 cataaaccac aaagcccatc acccccagcc agcaggccag cggattcgcg ccaaaccaca
    36121 gcagcataaa gccagcaata cccagcaagg tggcgtacac cagcgagaca gcaggagaga
    36181 tcaggccttt aaccagcacc cgattcttcg tcctttccat ctttctgtcg atatccctgt
    36241 cgatgtagtt gttaaacaca caacccgacg ccacaaccag tgacacccca accagcgtgt
    36301 agataaacag gggataatca atgctgccct ttgaggccag caggaatccc ccaatcaccg
    36361 agatcaggtt gccaaagatg atgcctggtt tcgttacttg caggtattgc ttaaacatca
    36421 taaccgccgc tcttagtgca tcatcatgtt gtagttgagg ttccacataa tccagatgga
    36481 gcctacaacc aggatagtga tgattagcac ggtgaagaca aacgccgtca tgttccagcc
    36541 ttcatctgat ttggtattca tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac
    36601 tgccattgcc aggattgttc ccagaattac ggccggagag gcagcccctg tcatcaccat
    36661 ccagaacgga atcaccgtca gaatgatcga caggataaag cctgtcatgt aggtttttac
    36721 gctgccatgg gacgcgccgc tgtgatcggt agaatgactc attacatcgc ccccatcaga
    36781 taaacaacag tgaacacaca gatccaaacc acatccagga agtgccagaa caggctcagg
    36841 cacatgatgc gggtacggtt agtgctggtc aggccgcgac gagcgatttg taccatcagc
    36901 accgccatcc agataagacc ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag
    36961 aacgctgaca ggaagccgct gcgatccgga cccatgccgt taacaatcag gtgatggaat
    37021 tcgtagattt ccatcccgat aaatccggca ccaaacaaac aggtcaacgc cagccaggag
    37081 ataacctggc ttttgttgtt tttgtacatg gcgatagccg ccatgccgta ggtgatggag
    37141 ctgaacaaca gcaagaaagt ttcaaccaga acgaacggca gttcgaaaat gtccttacct
    37201 gtcgggccgc ctgcggtgcc gttcaccaga acggcatagg tagcaaacaa gattgagaac
    37261 agaatgcagt cgctcatcag gtagatccaa aatccgaaga ttttggttcc gcctgcatcg
    37321 tggtgcccgt gttcgtgcgc gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt
    37381 cagccctgcc ttagtaatct catcgaaatg ctggttttcc agtttttcga tttctgccac
    37441 cggcacgtag taatccacgt cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc
    37501 tgcgaagcca acaatcgcca gccaccagat atgccagatc atggcgaaac cgaagatggt
    37561 ggagaaagct gcaatgacga tacctgcacc gctgtttttc ggcatatgaa tttcttcata
    37621 gtggtcaggc tttttatacg cttcgccttt ctctttcatt tcccagaatg catcacgctc
    37681 gtgaacgtgc ggcacaacgg caaagttata gaacggaggc ggggaagagt ttgcccactc
    37741 cagcgtacgg ccaccccacg ggtcgccagt caggtcacgg ttctggtcgc ggtcgcgaat
    37801 agaaacgtac atctgaataa cgaggcagag aatacccagc gcaatcagta ctgcaccgct
    37861 agctgcaatc atcagcatgg tgtggaactg cgggtcaatc tgctggctca aacgacgggt
    37921 catgcccatg aagcccagcg catacagcgg cataaaggca acgaagaagc cgatgatcca
    37981 gaaccagaac gcgcgtttac cccaggtttc attcagtttg aaaccgaacg ctttaggcca
    38041 ccagtaggtc atccctgcga agcagccgaa gaccacgccg ccgatgataa cgttatggaa
    38101 gtgcgcaatc aggaacaggc tgttatgcag aacgaagtcc gcgcccggta ctgccagcag
    38161 cacgccagtc atcccgccca ccgagaaagt gacgataaaa ccgatggtcc acagcatcgc
    38221 agaatggaac acgatacggc cctgatacat ggtgaacagc cagttgaaga tcttcacccc
    38281 ggtcgggatg gcgataatca ttgtggtgat accaaagaag gcgtttacgt tcgcgcccgc
    38341 acccatcgta aagaagtggt gcagccaaac gatgaacgac agcacggtga tacagacggt
    38401 tgcccatacc agcgaggtat aaccaaacag acgtttacgc gagaaggttg ccgcaatttc
    38461 ggagaacaca ccgaaaacag gcaggatcag gatgtaaact tccgggtggc cccaggccca
    38521 aatcaggttg atgtacatca tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc
    38581 cagatagcga tccagggtca acaacgcgac ggtaaccgtc agaattggga aggaagcaat
    38641 aatcagtacg ttcgcgcaca gtgatgccca ggtaaatact ggcatcttga acatggtcat
    38701 gcccggtgcg cgcatcttca gaatggtaac gaagaagttg ataccggtaa gcgtcgtacc
    38761 tataccggat agctggagac tccatatcca gtaatcaaca ccgactcccg gactgtactc
    38821 tattcccgat agcggtggat aggccagcca gccggtctgc gcaaattcgc ccacgccgag
    38881 agaaacgtta accagaatca caccaacaac agtaaaccag aagcttaagt tgttgaggaa
    38941 cgggaacgca acgtcacgcg cgccgatctg cagcggaacc accaggttca tcagaccgat
    39001 aacgaaaggc atcgccacga agaagatcat aatcacgccg tgcgcggtga agatctgatc
    39061 gtagtggtga ggtggcagga agcccgcttc gcccgccgag gcaagagcct gctggctacg
    39121 catcataatg gcgtcagcaa aaccacgcag caacatcaca atcgccacga tgatatacat
    39181 gataccgagg cgtttatggt cgacggaggt cagccactct ttccacaggt aggtccactt
    39241 accgaagtaa gtgatcaggc caacgagcgc cagacctccc aaaataatgc cagcgatcgt
    39301 aaccatgacg ataggttcat ggaacgggac tgcatcaagt gataattttc cgaacatctt
    39361 tattcttcct caaccccttt aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat
    39421 accttcgtgt gcgctgtgct caccttctgg ctgggtcatg tccatgctct taccgtgagc
    39481 cataaacttg ttaatcacat cagcaaacaa gtctggtttc acgttggaga aatattccac
    39541 ctggttgtat tcgctaggcg cggccagttt ttcgaacgca gccatgtcag acatggtgtt
    39601 cggcgactgc ttcgcttttg cgacccactg gtcgaattcg gcgcgatccg gtgttgcaat
    39661 agctttgaac ttcatgcctg agaagcccgg gccgctgtag ctggcggaga taccgtcata
    39721 agtaccgggt tcgttggcga tcagatgcag gcgagtctgc ataccagcca tggcataaat
    39781 ctggctaccc agacgcggaa tgaagaagga gttcatcacg gagttggagg tcactttgaa
    39841 gtacaccgga gtgttcgccg ggaaagcgat ttcattcacg gtagcaatgc cctgttccgg
    39901 gtagatgaag aaccatttcc agtccatgga aaccacttcg atggtaatgg gcttctcgtc
    39961 gtgtgccagc ggcttgctag gctcaagagc gtgagtggtt ttccaggtca gtaccgcaag
    40021 gaagatgatg attaagatag gtaccgtcca gaccacagct tccactttat tggagtgtga
    40081 ccagttcggg ctgtacttag catctttatt gctcgcacga tacttccagg cgaaaccaac
    40141 agccatcaag attgcgggaa taacgacaat caacatcagg ccaaatgccg tcagtatcag
    40201 tgaacgttgc tccagaccaa tctgtccttt gggatctaac agcgcagaat tacagccact
    40261 gagcaataca gtgcctgcaa ataatgacaa ccatcccaaa cttttattgt atttcctgag
    40321 tctcatttaa cgacctcaat tccacgggat ctggtggcgt ttaaagtgtg tgggcatttt
    40381 atgggaaggt tacattactg taaacatgat taaattcctg ttacttggat ttgattgtca
    40441 ggtcacatat gttgcaaaac atatcaagtt gattttaaga agctgttgca aaagggcgtt
    40501 ataacgaaag gggagtgcag aaggagtttg gatgggcaat tggtataacc aatctggaat
    40561 aaaataattg tttaacaaat aattatcatt atggtgacaa ttgggtgggg ggaaaataaa
    40621 gactcgttag ctgaatcgtg taagaaaaaa tagaattcat aaattgcgtc aataatttcc
    40681 aaacgctaag ccgcacaaaa gaacaaatat tattaagcga tactgatgtt tatttagccg
    40741 taataattac ggcgagtgat tactacagct aaataatatt tacagattac gtcagatgcg
    40801 tttttcgtag agccagataa tccagcaaac caccaagcac gacgccagaa agggcgacta
    40861 aaaccccgac ttccagcaga gcaggcagga aagagaagtg cgtcaaatcc agcgcgtcca
    40921 tcgtcaacag cagtaaccac acggccaaca ggctgacgcc cgccgccagt gtccacatgg
    40981 caaaggcata acctgccgga tattcggtac gggagataaa gttgtcattt actcgtgtat
    41041 attcaagcgt ctggcggcaa accagcagca aaataagccc cggtacggca gcggcgacgg
    41101 agaatagata gaatgtcgac cagccgtgtg cttcaacaaa ccaacccgcc acggggccga
    41161 cataaactcg tcctacagcg gaaagcgctg agagcagggc aaattgagta gcggaaaatg
    41221 acttattaca cagcgtcatt aacagcgcga caaaggctga tgtgcccatc ccgccacaga
    41281 ggttttcgaa aaagacggct gcgcccatgc tgtagagatg cttatcagta atcgacagca
    41341 gccagtaacc agcgttagac gcaccttgta aaatgccgaa aatcagcagc gcccggaaca
    41401 gcgacaggcg ctgcatcaaa atcccaccgt acaatgcgcc cacaatggtc gccagtaagc
    41461 caagcgtttt gttaaccacg cctacttcac ccgcatcaaa cccgacgccg cgaatcaaaa
    41521 acgtggttgt caggctcatg gcgaatgcgt cgcccagctt atacagcacg ataagaagca
    41581 aaataagcca ggcattattg cgaccaaaga aatctcgcag aggtgcaaca accgcttgtt
    41641 ccagcgtttt tggcacagga atggtgtcgg tcggttctgg tgcaagcaac gtcgcgataa
    41701 tacaagggat caacagcgcc gccatcaacc agtacatgcc ctgccagccc agccatttat
    41761 ctgccagcca cagggctaag ccgccggaaa ccagcatccc taaacggtaa cccagcacgc
    41821 tgattgccgc gcccgcacca cgttcttctg ccgggagcac atcggttttc cacgcatcga
    41881 agacaatatc ctgggaggcc gagcaaaaag cgatcaccac cgccagtgcc gccatccagc
    41941 ggagttgggt gccaggttcg agaaaaccca tcgccgcaat ggcgactaat aacaggattt
    42001 gcgtggcgag cagccaaccg cgccgccgcc caaaaaatgg gggcgtgtag cggtccatca
    42061 gcggtgacca gaggaattta aaaacgtaag cctggcctac cagagagaag aaaccaatgg
    42121 ttttgagatc aatattctcg accgtcatcc aagcctgtaa ggtaccggat gtcagggcga
    42181 gcggtagccc ggaagcaaaa cccaggatca gcaatatggc tgaacgcggc tgttgaaaaa
    42241 tacgtaaata ttgactggac atgggcgtct acaggcccgg tttgcaccgg gccagagggc
    42301 agaaattaac gcgcgttctg cttgatgaat tcgtgaatgc tggtgtcctg agacatatca
    42361 gcgatggtat cggtcagcac actgttaacc gcatcggcga tatttttgtt ggaggcctgg
    42421 aacgcacctt caacgttgta gctggcacga tagtttttgg ttattttgtt gccattctgc
    42481 gcggtagcga tgatggcgat atccgctttg gtagcgatgt tgtagcgcac gttgccctgg
    42541 gacacgtcag catacagttg gctaacgatg atttgcagat taaccgggcc attcggacca
    42601 accatgtaac cacgcgcggt catctgtttt tccagcactt cttgcagcag gaaacgcaga
    42661 tcgcgggagg cggccagggt aacgatttga ttatcgcggg tgacttttgc cagcgcctga
    42721 tcggtacgct gatcggcacc attaatgctt acggtgacgc ccatcaggct tggatcctgc
    42781 tgtggcagtg taatcgtcgg ggaaacttca atagttgttg gcggttttgc gcatcctgca
    42841 agcataaaca gagcaactaa cgggaagagg atttttttga acatgttcgg gctctcagag
    42901 actcttaagc gtgtttggta aaaattcccg ccatcataac attgccaacg gcgaggggaa
    42961 gtgggtaagg catgtaaatt catcatgttg acgaaataat cacccctggt aaaagaaacg
    43021 ctgatgcgag gcctgtgttt caatctttaa atcagtaaac ttcatacgct tgacggaaaa
    43081 accaggacga aacctaaata tttgttgtta agctgcaatg gaaacggtaa aagcggctag
    43141 tatttaaagg gatggatgac atctcagcgt tgtcggagga gatatttcat gatgatacgt
    43201 gagcggatag aagaaaaatt aagggcggcg ttccaacccg tattcctcga agtagtggat
    43261 gaaagctatc gtcacaatgt cccagccggc tctgaaagcc attttaaagt tgtgctggtc
    43321 agcgatcgtt ttacgggtga acgttttctg aatcgtcatc gaatgattta cagtacttta
    43381 gcggaggaac tctctactac cgttcatgcg ctggctctgc atacttacac tattaaggag
    43441 tgggaagggt tgcaggacac cgtctttgcc tctcctccct gtcgtggagc aggaagcatc
    43501 gcgtaaaaac gcatttgcaa ctgtcggcgc tttttcagta tgttgctaaa gattttatga
    43561 aaaacggcct gcgggccgtt ttgttttgtc tggattttgc gctttttgcc cagcattcag
    43621 acgaaaattg cccgggaatt gtgaaaaaat acgcgacagc gcgcaataac cgttctcgac
    43681 tcataaaagt gatgccgcta taatgccgcg tcctatttga atgctttcgg gatgattctg
    43741 gtaacaggga atgtgattga ttataagaac atcccggttc cgcgaagcca acaacctgtg
    43801 cttgcggggt aagagttgac cgagcactgt gattttttga ggtaacaaga tgcaagtttc
    43861 agttgaaacc actcaaggcc ttggccgccg tgtaacgatt actatcgctg ctgacagcat
    43921 cgagaccgct gttaaaagcg agctggtcaa cgttgcgaaa aaagtacgta ttgacggctt
    43981 ccgcaaaggc aaagtgccaa tgaatatcgt tgctcagcgt tatggcgcgt ctgtacgcca
    44041 ggacgttctg ggtgacctga tgagccgtaa cttcattgac gccatcatta aagaaaaaat
    44101 caatccggct ggcgcaccga cttatgttcc gggcgaatac aagctgggtg aagacttcac
    44161 ttactctgta gagtttgaag tttatccgga agttgaactg cagggtctgg aagcgatcga
    44221 agttgaaaaa ccgatcgttg aagtgaccga cgctgacgtt gacggcatgc tggatactct
    44281 gcgtaaacag caggcgacct ggaaagaaaa agacagcgct gttgaagcag aagaccgcgt
    44341 aaccatcgac ttcaccggtt ctgtagacag cgaagagttc gaaggcggta aagcgtctga
    44401 tttcgtactg gcgatgggcc agggtcgtat gatcccgggc tttgaagacg gtatcaaagg
    44461 ccacaaagct ggcgaagagt tcaccatcga cgtgaccttc ccggaagaat accacgcaga
    44521 aaacctgaaa ggtaaagcag cgaaattcgc tatcaacctg aagaaagttg aagagcgtga
    44581 actgccggaa ctgactgcag aattcatcaa acgtttcggc gttgaagatg gttccgtaga
    44641 aggtctgcgc gctgaagtgc gtaaaaacat ggagcgcgag ctgaagagcg ccatccgtaa
    44701 ccgcgttaag tctcaggcga tcgaaggtct ggtaaaagct aacgacatcg acgtaccggc
    44761 tgcgctgatc gacagcgaaa tcgacgttct gcgtcgccag gctgcacagc gtttcggtgg
    44821 caacgaaaaa caagctctgg aactgccgcg cgaactgttc gaagaacagg ctaaacgccg
    44881 cgtagttgtt ggcctgctgc tgggcgaagt tatccgcacc aacgagctga aagctgacga
    44941 agagcgcgtg aaaggcctga tcgaagagat ggcttctgcg tacgaagatc cgaaagaagt
    45001 tatcgagttc tacagcaaaa acaaagaact gatggacaac atgcgcaatg ttgctctgga
    45061 agaacaggct gttgaagctg tactggcgaa agcgaaagtg actgaaaaag aaaccacttt
    45121 caacgagctg atgaaccagc aggcgtaatt tacgcagcat aacgcgctaa attcgcacaa
    45181 aggcccgtca ccgccaggtg gtgggctttt tttgtcatga attttgcatg gaaccgtgcg
    45241 aaaagcctct ttcggtgtta gcgtaacaac aaaagattgt tatgcttgaa atatggtgat
    45301 gccgtaccca taacacaggg actagctgat aatccgtcca taaggttaca atcggtacag
    45361 caggtttttt caattttatc caggagacgg aaatgtcata cagcggcgaa cgagataact
    45421 ttgcacccca tatggcgctg gtgccgatgg tcattgaaca gacctcacgc ggtgagcgct
    45481 cttttgatat ctattctcgt ctacttaagg aacgcgtcat ttttctgact ggccaggttg
    45541 aagaccacat ggctaacctg attgtggcgc agatgctgtt cctggaagcg gaaaacccag
    45601 aaaaagatat ctatctgtac attaactccc caggcggggt gatcactgcc gggatgtcta
    45661 tctatgacac catgcagttt atcaagcctg atgtcagcac catctgtatg ggccaggcgg
    45721 cctcgatggg cgctttcttg ctgaccgcag gggcaaaagg taaacgtttt tgcctgccga
    45781 attcgcgcgt gatgattcac caaccgttgg gcggctacca gggccaggcg accgatatcg
    45841 aaattcatgc ccgtgaaatt ctgaaagtta aagggcgcat gaatgaactt atggcgcttc
    45901 atacgggtca atcattagaa cagattgaac gtgataccga gcgcgatcgc ttcctttccg
    45961 cccctgaagc ggtggaatac ggtctggtcg attcgattct gacccatcgt aattgatgcc
    46021 agaggcgcaa ctgtgccgct atacttatcc agggcggcac aacgctgtaa gcggcttgcg
    46081 cctgagaatg gcatttgcgt cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc
    46141 catgacagat aaacgcaaag atggctcagg caaattgctg tattgctctt tttgcggcaa
    46201 aagccagcat gaagtgcgca agctgattgc cggtccatcc gtgtatatct gcgacgaatg
    46261 tgttgattta tgtaacgaca tcattcgcga agagattaaa gaagttgcac cgcatcgtga
    46321 acgcagtgcg ctaccgacgc cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg
    46381 ccaggaacag gcgaaaaaag tgctggcggt cgcggtatac aaccattaca aacgtctgcg
    46441 caacggcgat accagcaatg gtgtcgagtt gggcaaaagt aacattctgc tgatcggtcc
    46501 gaccggttcc ggtaaaacgc tgctggctga aacgctggcg cgcctgctgg atgttccgtt
    46561 caccatggcc gacgcgacta cactgaccga agccggttat gtgggtgaag acgttgaaaa
    46621 catcattcag aagctgttgc agaaatgcga ctacgatgtc cagaaagcac agcgtggtat
    46681 tgtctacatc gatgaaatcg acaagatttc tcgtaagtcg gacaacccgt ctattacccg
    46741 tgacgtttcc ggtgaaggcg tacagcaggc actgttgaaa ctgatcgaag gtactgttgc
    46801 tgccgttcca ccgcagggcg ggcgtaagca tccgcagcag gaatttttgc aggttgatac
    46861 ctccaagatc ctgtttattt gtggcggtgc gtttgccggt ctggataaag tgatttccca
    46921 ccgtgtagaa accggctccg gcattggttt tggcgcgacg gtaaaagcga agtccgacaa
    46981 agcaagcgaa ggcgagctgc tggcgcaggt tgaaccggaa gatctgatca agtttggtct
    47041 tatccctgag tttattggtc gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga
    47101 agctctgatt cagatcctca aagagccgaa aaacgccctg accaagcagt atcaggcgct
    47161 gtttaatctg gaaggcgtgg atctggaatt ccgtgacgag gcgctggatg ctatcgctaa
    47221 gaaagcgatg gcgcgtaaaa ccggtgcccg tggcctgcgt tccatcgtag aagccgcact
    47281 gctcgatacc atgtacgatc tgccgtccat ggaagatgtc gaaaaagtgg ttatcgacga
    47341 gtcggtaatt gatggtcaaa gcaaaccgtt gctgatttat ggtaagccgg aagcgcaaca
    47401 ggcatctggt gaataattaa ccattcccat acaattagtt aaccaaaaag gggggatttt
    47461 atctcccctt taatttttcc tctattctcg gcgttgaatg tgggggaaac atccccatat
    47521 actgacgtac atgttaatag atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg
    47581 aaattaaact aagagagagc tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg
    47641 tattgccgct gcgcgatgtg gtggtttatc cgcacatggt cattccctta tttgtcgggc
    47701 gggaaaaatc tatccgttgt ctggaagcgg cgatggacca tgataaaaaa attatgctgg
    47761 tcgcgcagaa agaagcttca acggatgagc cgggtgtaaa cgatcttttc accgtcggga
    47821 ccgtggcctc tatattgcag atgctgaaac tgcctgacgg caccgtcaaa gtgctggtcg
    47881 aggggttaca gcgcgcgcgt atttctgcgc tctctgacaa tggcgaacac ttttctgcga
    47941 aggcggagta tctggagtcg ccgaccattg atgagcggga acaggaagtg ctggtgcgta
    48001 ctgcaatcag ccagttcgaa ggctacatca agctgaacaa aaaaatccca ccagaagtgc
    48061 tgacgtcgct gaatagcatc gacgatccgg cgcgtctggc ggataccatt gctgcacata
    48121 tgccactgaa actggctgac aaacagtccg ttctggagat gtccgacgtt aacgaacgtc
    48181 tggaatatct gatggcaatg atggaatcgg aaatcgatct gctgcaggtt gagaaacgca
    48241 ttcgcaaccg cgttaaaaag cagatggaga aatcccagcg tgagtactat ctgaacgagc
    48301 aaatgaaagc tattcagaaa gaactcggtg aaatggacga cgcgccggac gaaaacgaag
    48361 ccctgaagcg caaaatcgac gcggcgaaga tgccgaaaga ggcaaaagag aaagcggaag
    48421 cagagttgca gaagctgaaa atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg
    48481 gttatatcga ctggatggta caggtaccgt ggaatgcgcg cagcaaggtc aaaaaagacc
    48541 tgcgtcaggc gcaggaaatc cttgataccg accattatgg tctggagcgc gtgaaagatc
    48601 gcatccttga gtaccttgcg gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct
    48661 gcctggtagg gccgccgggg gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca
    48721 ccgggcgtaa atatgtccgt atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg
    48781 gtcaccgccg tacttacatc ggttctatgc cgggtaaact gatccagaaa atggcgaaag
    48841 tgggcgtgaa aaacccgctg ttcctgctcg atgagatcga caaaatgtct tctgacatgc
    48901 gaggcgatcc ggcctctgca ctgcttgaag tgctggatcc agagcagaac gtagcgttca
    48961 gcgaccacta cctggaagtg gattacgatc tcagcgacgt gatgtttgtc gcgacgtcga
    49021 actccatgaa cattccggca ccgctgctgg atcgtatgga agtgattcgc ctctccggtt
    49081 ataccgaaga tgaaaaactg aacatcgcca aacgtcacct gctgccgaag cagattgaac
    49141 gtaatgcact gaaaaaaggt gagctgaccg tcgacgatag cgccattatc ggcattattc
    49201 gttactacac ccgtgaggcg ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgcc
    49261 gcaaagcggt taagcagtta ctgctcgata agtcattaaa acatatcgaa attaacggcg
    49321 ataacctgca tgactacctc ggtgttcagc gtttcgacta tggtcgcgct gataacgaaa
    49381 actgtgtcgg tcaggtaacc ggtctggcgt ggacggaagt gggcggtgac ttgctgacca
    49441 ttgaaaccgc atgtgttccg ggtaaaggca aactgaccta taccggttcg ctcggcgaag
    49501 tgatgcagga gtctattcag gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg
    49561 ggatcaaccc tgatttttac gaaaaacgtg acatccacgt ccacgtaccg gaaggtgcga
    49621 cgccgaaaga tggtccgagt gccggtattg ctatgtgcac cgcgctggtt tcttgcctga
    49681 ccggtaaccc ggttcgtgcc gatgtggcaa tgaccggtga gatcactctg cgtggtcagg
    49741 tactgccgat cggtggtttg aaagaaaaac tcctggcagc gcatcgcggc gggattaaaa
    49801 cagtgctaat tccgttcgaa aataaacgcg atctggaaga gattcctgac aacgtaattg
    49861 ccgatctgga cattcatcct gtgaagcgca ttgaggaagt tctgactctg gcgctgcaaa
    49921 atgaaccgtc tggcatgcag gttgtgactg caaaatagtg acctcgcgca aaatgcacta
    49981 ataaaaacag ggctggcagg ctaattcggg cttgccagcc tttttttgtc tcgctaagtt
    50041 agatggcgga tcgggcttgc ccttattaag gggtgttgta aggggatggc tggcctgata
    50101 taactgctgc gcgttcgtac cttgaaggat tcaagtgcga tataaattat aaagaggaag
    50161 agaagagtga ataaatctca attgatcgac aagattgctg caggggctga tatctctaaa
    50221 gctgcggctg gccgtgcgtt agatgctatt attgcttccg taactgaatc tctgaaagaa
    50281 ggggatgatg tagcactggt aggttttggt acttttgccg ttaaagagcg tgctgcccgt
    50341 actggccgca acccgcagac cggtaaagag atcaccatcg ctgctgctaa agtaccgagc
    50401 ttccgtgcag gtaaagcact gaaagacgcg gtaaactaag cgttgtcccc ggtggggatg
    50461 tgacgaagtt caagggcgca tctactgatg tgcctttttt atttgtattc ggtgactttc
    50521 tgcgtcttgt gggctgacaa ttgcccccgt ttcttgtcac aataggcctt tgcgcgcatc
    50581 gatacgttgc gtgaggtaca cagtcatcta cagcggagtg ttgttacacc atgatggaca
    50641 gcttacgcac ggctgcaaac agtctcgtgc tcaagattat tttcggtatc attatcgtgt
    50701 cgttcatatt gaccggcgtg agtggttacc tgattggcgg aggcaataac tacgccgcaa
    50761 aagtgaatga ccaggaaatc agccgtgggc agttcgagaa cgcctttaac agcgagcgta
    50821 atcgcatgca gcaacagctg ggcgatcaat actccgagct ggcagcgaat gaaggctata
    50881 tgaaaaccct gcgtcaacag gtgctgaatc gtctgatcga cgaggcgctg ttggatcagt
    50941 acgctcgtga gctgaaactg ggtatcagtg atgagcaggt taaacaggcg attttcgcga
    51001 ccccagcctt ccaggttgac ggcaaatttg ataacagccg ctataacggt atcctcaacc
    51061 agatggggat gaccgccgat cagtacgccc aggcgctgcg taaccagctc actacccaac
    51121 agctgattaa cggtgttgcc ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg
    51181 cggcactggt cgcgcaacaa cgcgtggtgc gtgaggcgac tatcgatgtt aacgcgctgg
    51241 cggcgaagca gcctgtgacc gaacaggaga ttgccagcta ctacgaacaa aacaaaaaca
    51301 atttcatgac gccggaacaa ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc
    51361 agcaaccggt tagcgatgcg gatatccaga gctactacga tcagcatcag gatcaattca
    51421 cccagccgca gcgtacccgc tacagcatca tccagaccaa aactgaagat gaagcgaaag
    51481 cggtacttga tgagctgaat aaaggcggtg attttgctgc attagccaaa gaaaaatctg
    51541 ccgatattat ctctgcccgt aacggcggcg atatgggttg gttagaagat gccactatcc
    51601 ccgacgaact gaaaaatgct ggtctgaaag aaaaaggcca actgtctggt gtcatcaaat
    51661 cttcggtcgg tttcctgatt gtacgtctgg acgacattca gccagcgaaa gtgaaatcgt
    51721 tagacgaagt acgtgacgac attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt
    51781 actatgcgct gcagcagaaa gtgagcgatg cggcaagcaa cgacaccgag tctctggccg
    51841 gtgcagagca agctgccggc gttaaagcca ctcagacggg ttggttcagc aaagataacc
    51901 tgccggaaga gttgaacttc aagccggttg ccgacgctat ctttaacggc ggtctggtag
    51961 gtgaaaacgg cgcgccgggc atcaactctg acatcatcac cgtagacggc gaccgtgcat
    52021 tcgtgctgcg catcagcgag cacaaaccgg aagcggtgaa accgttggca gatgttcagg
    52081 aacaagttaa ggcactggtt cagcacaaca aagctgaaca acaggcgaaa gtggatgctg
    52141 agaaactgct ggttgatttg aaagccggca aaggtgcgga agctatgcag gctgccggtc
    52201 tgaaatttgg cgagccgaaa accttaagcc gttccggtcg tgacccgatt agccaggcgg
    52261 cgtttgcact gccactgcca gcgaaagaca aaccgagcta cggtatggcg accgatatgc
    52321 aaggtaatgt ggttctgctg gcgctggatg aagtgaaaca aggttcaatg ccggaagatc
    52381 agaaaaaagc gatggtgcag ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc
    52441 tgatgagtaa cctgcgtaaa gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat
    52501 aatcctgaag ccgcctcgca aaaaaatgcg ttgcagctgt aacaagaaaa ggtcgctttc
    52561 gcggcctttt ccatttctga acattgccat ttgtttactg ttttcactgc cgttaaggtg
    52621 attccactgt taacaaacaa ggagaaaaca gtatgaaaca cggaattaaa gcactgctca
    52681 ttaccctgtc cctggcctgt gccggaatgt ctcatagcgc gctggcggca gcttctgtgg
    52741 cgaaaccgac ggcggtagaa accaaagcgg aagctcctgc agcacaaagt aaagcagcag
    52801 taccggcgaa agccagtgac gaagaaggca cccgggtcag cattaataat gccagcgcgg
    52861 aagagctagc ccgcgcgatg aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt
    52921 atcgcgaaga gtacggtccg tttaaaacag tggaggatct aaagcaggtg ccggggatgg
    52981 gcaattcgct ggtggaacgt aatctggcgg tattaaccct gtaattaatt tgcatagtgg
    53041 caattttttg ccagactgaa gaggtcatac cagttatgac ctctgtactt ataacaacaa
    53101 cgtaaggtta ttgcgctatg caaacacaaa taaaagttcg tggatatcat ctcgacgttt
    53161 accagcacgt caacaacgcc cgctaccttg aatttctcga agaagcccgc tgggatgggt
    53221 tggaaaatag cgacagtttt cagtggatga cggcccataa catcgccttc gtcgtggtca
    53281 atatcaatat taactatcgt cgcccagcgg tattaagtga cctgttaact attaccagtc
    53341 agttgcagca attaaacggt aaaagcggca tcttaagcca ggtcattaca ctggagccgg
    53401 aagggcaggt ggtagcggat gcgcttatta cgtttgtttg tattgatctt aaaacgcaga
    53461 aagcattagc tctggaaggg gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt
    53521 tttgtggtgc cggatgctca agccgcatcc ggcgacaccc ggaataatta cctcaacccg
    53581 gttttctgct tcatcgctgc catcaccgtc ggtttatcgg ccagataatg attcaaaccg
    53641 ttggcgcgta aattacatgc cgcacaatga ccgcaaccgt cgcctttaat gccgttatag
    53701 caggtcaacg tttcgttacg gactaaatcc agtttgccgt aataatctgc cagcgcccag
    53761 gtttccgctt tatcaatcca catcagcggc gtttcaaaac gaatatcttt ctccatgccc
    53821 aaactgacgg catggtttag tgctttcaca aactcatcgc ggcaatccgg gtagccggag
    53881 aaatccgttt cacagacgcc ggtaattacg gcttctgctt ttacctgata cgcatatatt
    53941 gccgccagcg tcaggaacaa aatattacgc cctgggacaa acgtattcgg gatgccatcg
    54001 gcttcaggtt cataatcagg caccggaatg ctgtcacgtg tcaggctgct gaccgccagc
    54061 tcgttgagca gcgttacatc aagaacctta tgcgcgcgtg ctcccagttt cagcgccagt
    54121 tcgcgtgcca cgtcgatttc tgcgcgatgt cgctgaccgt aatcgaacgt cacgcaatgg
    54181 acttcatcat attgttgtaa tgcctgcacc agacaggtgg tggaatcctg gcctccactg
    54241 aacacaacga cagcacgttt catagataat cctgcttgaa caataaaagc gttatggtaa
    54301 cgcctgcgat taacccggac cagctattca ttgcgacggc gcgggaagcc aggcttcggt
    54361 aaattcaaac cagccgcgcg gtgtcagtcg cacaccgttc acgccgggag gggcactaat
    54421 gcgatagtga tagttgaaca gcggcgtcag cgtcgcgtct gccattaact ggctaaaaac
    54481 cgctttcagg gcattaaatc ggttttcctc atcaggcatt acttgcaccg cgtccagcgt
    54541 cgattgcaga tgtgcatatg ctggagcgtc gaaaacatgt ggccatagtg gatcgcaacg
    54601 tagccattgc tccagagtat attccggtgc ttcgccaatt aatctgtcgc ccatcatgag
    54661 gtctgcgtgt gccagtaggg tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt
    54721 gagttcacag ccttccgctg ccagtgtcgc ctgtaggcgt tctgccatgg tatgaagttc
    54781 tatcggtagg tgataaacca gcgtcaaggt tttcggtagt ttgacttcat ccggtacttg
    54841 ccagtgcgga atagtccagc ctggcagtaa tgcatgactg gcggtgatca ggttttctcc
    54901 gacttctaac gtttgtaata aaccggattg atgaataatg gagatcactt ttcgcgcctg
    54961 ccagagggag agtcggggac ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc
    55021 actactgacc tggctgaccc gttgcagctc ctccggtttg ccgatggtga tttgcacggg
    55081 atgccgacaa ctggttccca aatctttttc gaaaagcggc ggagttatcc agtactcaac
    55141 cgctttaagc agcggatgac gcaaatggta ataatcatgg ctctccaggc gcaccagctc
    55201 cgctgtgaat tgtgttaagc gaaaaggacc agtgccgatc agtgggaatt gcggatgcgc
    55261 cagatggctg caatagctcg ccagccggtg cgcaagccag taatcggggc gatgtaaaaa
    55321 gaaggtcaga cactgcggat gggtgacttc aatacgcttc acgctaataa ataattgatc
    55381 cagtgctggc agttgtaaca gcatcaataa tcgctggtgt aagtgtgagg cttttactgc
    55441 atcgccgtta tgccagtgta gggttgaacg aagataaaag tcccagcgta acccgtcagt
    55501 agaggtttcc cagtgatgcg ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg
    55561 ggtcaggccg gaaaatatct gcccggcgag atgctgctcg gcacggccgg gcaaaaagcc
    55621 tggttgtagc ggttcgagcg ggcgatagta gggaatacgc aacgtgggtg tatcgttttg
    55681 ccattgtccg cccataaacg gctgtaacag agtgcgtagc tcacctaggg ccagttgcgc
    55741 cagctccagc acatcttgct gctttccggt ttccagtgcc tgttccatca tcgcattgcg
    55801 tagcgattcc ggcgtgacca gaaagcgtaa ttgtccgcgc tttccgcgtc ctgactgcgc
    55861 ctgccactcc agccatcccg cctcctgtgc ctgacgcaac agcgtacgaa catggcgttc
    55921 gctgcaaaaa cagcgttcgg ccagttcgct gacggtgacg gtttgcggct ttccggctga
    55981 aggttgccac agacgttgat actggttaag acggttgagc aatcgcatat aaacccggaa
    56041 caatattatt taactattca ctattacttc cgtatatatc aggtgatact caatctccat
    56101 taaccgtgtc acagagtgga gaaagaaatg gctcgtctgg cagcatttga tatggatggc
    56161 actttattga tgcctgacca tcatttaggt gagaaaaccc tctctacttt ggcgcgactg
    56221 cgtgaacgcg acattaccct cacttttgcc acggggcgtc atgcgctgga gatgcagcat
    56281 attctcgggg cgctatcgct ggatgcatat ttgattaccg gcaacggaac gcgcgtgcat
    56341 tctctggaag gtgaactttt acatcgtgat gatttacctg cggatgtcgc ggagctggtg
    56401 ctgtatcagc aatgggatac ccgagccagc atgcatatct tcaatgacga cggttggttt
    56461 accgggaaag agatccctgc gttgttgcag gcatttgtct atagcggttt tcgttatcag
    56521 ataatcgatg tcaaaaaaat gccactcggc agcgtcacca agatctgctt ctgtggcgat
    56581 cacgacgatc ttacacgctt gcagatccag ctatacgaag cattaggcga gcgtgcacat
    56641 ttgtgttttt ccgccacgga ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct
    56701 gcattgacgg tgctgaccca acatctaggt ttatcgttgc gcgattgcat ggcctttggt
    56761 gatgcgatga acgatcgcga aatgttaggc agcgtcggta gcgggtttat tatgggcaat
    56821 gcgatgccgc aactgcgcgc ggagctcccg catttaccgg tgattggaca ttgccgaaat
    56881 taggctgtct ctcactattt gacgcactga ctggactatc cacatctacc ttattccccc
    56941 gaataatgag atcccttcca gcaccgggca attgcccggt tttttttgcg ttgaatttgt
    57001 cattttgtgc cgtggtgttt aaaccgcaca gaataaattg tcgtgatttc acctttaaaa
    57061 tagaattaaa agagaaaaaa attctctgtg gaagggctat gttagataaa attgaccgta
    57121 agctgctggc cttactgcag caggattgca ccctctcttt gcaggcactg gctgaagccg
    57181 ttaatctgac aaccacccct tgctggaagc gcctgaaacg gctggaggac gacggtatcc
    57241 ttatcggcaa agtcgccctg ctggatccgg aaaaaatagg cctcggcctg accgcctttg
    57301 tgctgataaa aacgcaacat cacagtagcg aatggtattg ccgctttgtc acggtggtta
    57361 ccgaaatgcc agaagtgctg gggttctggc gcatggctgg tgaatacgat tatctgatgc
    57421 gcgtccaggt tgccgacatg aaacgctacg acgagtttta taagcgtctg gtaaacagcg
    57481 tgccggggct gtcggacgtc acttccagct tcgcgatgga acagattaaa tacaccactt
    57541 ctttacccat cgaataaata tccagaatca ggtcaggaca caacgcgtgc gattatttgc
    57601 tcaattaagc tggtatttcc gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct
    57661 tgtcattatc gcgatgctgc aactggttcc gccaaaagtg gttggtattg ttgtcgatgg
    57721 cgtgacagaa caacacttta ctaccgggca gatcctgatg tggatcgcca ccatggtgct
    57781 gattgccgtt gtggtttatt tcctgcgtta cgtctggcgg gtattgctgt ttggtgcgtc
    57841 ttatcaactg gctgttgaac tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc
    57901 tgagttttac ctgcgtcatc gcacgggtga cctcatggct cgtgcgacca atgacgtcga
    57961 tcgcgtcgtg tttgcagccg gagaaggggt gctgacgctg gtggattcac tggtgatggg
    58021 ctgcgctgtg ttgattatga tgtctacgca aattagctgg cagttgacct tatttgccct
    58081 gttgccgatg ccagtgatgg cgatcatgat caagcgcaac ggcgatgcct tgcatgaacg
    58141 ctttaagctg gcacaggcgg cgttttctag tcttaatgac cgcacccagg aaagcctcac
    58201 cagtatccgc atgatcaaag cctttggtct ggaagatcgc cagtcggcgt tatttgccgc
    58261 ggatgccgaa gataccggca aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga
    58321 cccgaccatc tatatcgcga ttggtatggc gaatttgctg gcgattggcg gcggtagttg
    58381 gatggtggtg cagggcagtt taacgctggg ccagctcacc agttttatga tgtatttagg
    58441 tctgatgatt tggccaatgc tggcgctggc atggatgttt aacattgtgg aacgtggtag
    58501 tgctgcatac agccgtattc gcgcgatgct ggcggaagcg ccggtggtga acgatggtag
    58561 cgaacccgtg ccggaagggc gtggcgaact ggatgtaaat attcaccagt tcacgtatcc
    58621 gcagactgac catcctgcgc tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct
    58681 gggtatctgc gggccgactg gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca
    58741 tttcgacgtc agcgaggggg atattcgctt tcatgatatt cctctgacga agttacaact
    58801 cgatagctgg cgtagccgcc tggcggtagt tagccagaca ccgttccttt tttccgacac
    58861 cgtagcgaac aacatcgcgt tgggttgccc gaatgccacc cagcaagaga ttgagcatgt
    58921 cgcgcggtta gccagcgtac atgacgatat tttgcgtcta ccgcaaggtt acgatacaga
    58981 agtgggcgag cgcggcgtga tgctttccgg tgggcaaaaa cagcgtatct ccattgctcg
    59041 agcgttatta gtcaacgcgg aaatcctcat ccttgatgat gcgctttcgg cggtggacgg
    59101 acgcacagag caccagatcc tgcataacct gcgtcagtgg ggacagggaa gaacggtaat
    59161 catcagtgcc catcgccttt cggcactgac ggaagccagt gaaaatattg tgatgcagca
    59221 cggacatatc gcccagcgtg gcaatcatga tgagctggcg caacaaagcg gctggtatcg
    59281 cgatatgtat cgctatcaac aactggaggc ggcgctcgac gacgctccgg aaattcgcga
    59341 ggaggccgtc gatgcgtagt tttagccaac tgtggccgac tctcaagcgt ctgttagcgt
    59401 acggttcgcc gtggcgtaaa ccgctgggga ttgcggtcct gatgatgtgg gttgcggcag
    59461 cggcagaagt cagtgggccg ctgcttatca gctattttat cgacaatatg gtagcgaaaa
    59521 ataacctgcc gttgaaaatg gttgcagggc tggctgcggc gtatgttggg ctacaactgt
    59581 ttgccgccgg gctacattac gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag
    59641 tgcaacagtt gcgtaccgac gtgatggatg ccgcattgcg ccagccatta agcgagtttg
    59701 atacccaacc cgtcgggcag gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg
    59761 atctctacgt taccgtagtg gcaactgtcc tgcgtagcgc cgcactggtg ggcgcgatgc
    59821 tggtggcgat gttcagcctc gactggcgaa tggcgctggt ggcgataatg attttcccgg
    59881 tggtgctggt ggtaatggtg atataccagc gttacagcac gccgattgtc cgtcgtgtgc
    59941 gcgcctatct ggcggatatc aacgacggct ttaacgaaat catcaatggc atgagcgtta
    60001 tccagcagtt ccgtcagcag gcgcgatttg gcgaacgtat gggggaggcc agtcgttcac
    60061 actatatggc caggatgcaa accctgcgcc tcgacggttt tctgctgcgt ccgctgctga
    60121 gtctgttttc atcgctcatt ctttgtggct tgttgatgct gtttggcttc tccgctagcg
    60181 gcaccattga agtaggcgtg ctgtatgcgt ttatcagcta tcttgggcga cttaacgaac
    60241 ccttaatcga actgaccacg caacaggcga tgctgcaaca ggctgttgtt gctggtgagc
    60301 gcgtgtttga actgatggac ggaccgcgcc agcaatatgg caatgatgat cgcccgttac
    60361 agagtggcac catcgaagtc gataacgtgt catttgctta tcgcgatgac aatctggtgc
    60421 taaagaacat taatctctct gtgccttcgc gtaactttgt ggcactggtc gggcataccg
    60481 gcagtggcaa aagcaccctc gccagtttat tgatgggcta ttacccgcta acggaaggtg
    60541 agattcgcct tgatggtcgt ccattaagtt cgctaagtca cagcgcgctg cgccagggcg
    60601 tggcaatggt gcagcaagat ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc
    60661 tggggcgtga tatctccgaa gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg
    60721 agctggcgcg cagcatgagt gatggtattt atacgccgct gggcgagcag gggaataatc
    60781 tctccgtcgg gcaaaagcaa ctgctggcac tggcacgcgt gttggtggag acgccgcaaa
    60841 tcctgatcct tgatgaggca accgccagca ttgactccgg tactgaacag gcgattcaac
    60901 atgctctggc ggctgtgcgt gaacatacca cgctggtggt gattgctcac cgcttatcga
    60961 ccattgttga tgccgacacc attctggtgc ttcatcgtgg gcaagccgtg gagcagggca
    61021 ctcaccagca actgctggcg gcccagggac gctactggca gatgtatcaa ctgcaacttg
    61081 cgggcgaaga gctggcagcc agcgtgcgtg aagaagaatc attgagcgcc tgaatagcgc
    61141 aatatttcat cgttggtgca aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat
    61201 tttcaatctt cttaacttcc tgctctcttt ctcgtttttc atttctggca caccgcttgc
    61261 aataccttct tcgtgtagca gaaccattac cgaattctga ctggagggga tctatgaagc
    61321 tggtgaccgt gataatcaaa ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca
    61381 ttggtattca gggcctgacc gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg
    61441 ccgagctgta ccggggggcg gaatacagcg tcaacttcct gccaaaagta aaaattgatg
    61501 tggcaattgc tgatgaccaa ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca
    61561 ccggaaaaat tggcgacggc aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc
    61621 gtaccggcga agccgacgaa gcggcgctgt aatctctggc acacagcaac aggaacgaaa
    61681 aatgaagata gcgacgataa aaactgggct tgcttcactg gcgatgctgc cgggactggt
    61741 aatggctgca cctgcggtgg ccgataaagc cgacaatgcg tttatgatga tttgtactgc
    61801 gctggtgctg tttatgacta ttccggggat tgccctgttt tacggtgggt tgattcgcgg
    61861 caaaaacgtg ctgtcgatgc tgatgcaggt gacggtgacg tttgcactgg tctgtattct
    61921 ctgggtggtt tacggttact cgctggcgtt tggtgagggt aacaacttct tcggcaacgt
    61981 taactggttg atgctgaaaa acatcgaact gacggcggtg atgggcagca tttatcagta
    62041 tatccacgta gcgtttcagg gatcgtttgc ctgcattacc gtaggcttga tagttggggc
    62101 gctggcggaa cgaatccgct tctcagctgt gttgattttc gtggtggtat ggctgacgct
    62161 ctcttacatt ccgattgcgc atatggtgtg gggcggcggt ttgctggctt ctcacggtgc
    62221 actggatttc gcgggtggca cagtggtgca cattaacgcc gcaatcgccg gtctggtggg
    62281 cgcgtatctg ataggcaaac gcgtgggctt cggtaaagag gcgtttaaac cgcacaacct
    62341 gccgatggtc ttcaccggga ctgccattct ctatatcggt tggtttggct ttaacgccgg
    62401 gtcagcgggc acggcgaatg aaatcgcggc actggcattt gtgaatactg tggtcgcaac
    62461 ggcggcggca attcttggct ggatcttcgg tgaatgggcg ctgcgtggta agccttcact
    62521 gctgggggcg tgttctggcg cgattgccgg tctggtcggc gtgacgccag cgtgcggcta
    62581 cattggggtt ggcggcgcgt tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg
    62641 cgttaccatg ctcaaacgct tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca
    62701 cggcgtttgt ggcattgtcg gctgtatcat gaccgggatt tttgccgcca gctcgctggg
    62761 cggcgtgggc ttcgctgaag gtgtgacgat gggtcatcag ttgctggtac agctggaaag
    62821 catcgccatt acgatcgtct ggtccggtgt ggtggcattt attggctaca aattggcgga
    62881 tctgacggtt ggtctgcgtg taccggaaga gcaggagcta gaagggctgg atgtcaacag
    62941 tcacggcgag aatgcctata acgcgtaaca agcactgcaa aaaacagccg gacggttttc
    63001 acctccggct atttttttaa ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa
    63061 ccagtacgcc gtcctgggta taaaactcac cgcgcacaaa gccacgtgcg ctggacgccg
    63121 aggtgctctc cacgctatac agcagccatt cattcaaatt aaacgggcga tggaaccaca
    63181 tggaatggtc aatggtggca atctgaatcc ccggttcgag aaaaccgatg ccgtgcggct
    63241 gtagagctac cggcaggaag ttaagatcag aagcgtaacc gagcagatac tgatgaacgc
    63301 gcaggtcatc cggcacgcta ccatttgcgc ggatccacac ctgacgatgt ggttctgcga
    63361 cgtgaccttt cagtgggtta tgaaactcca ccggacggac ttccagcgga cgatcgcaga
    63421 tgaatttatc tttaagcact ggcggcagca ggtgcgccag cgattgggcg atttgcgttt
    63481 ccgaaggcag gccatcaggc gctggcgcgg acggcattgt tttttgatgt tcgaaacccg
    63541 cttctggtgc ctggaaagag gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag
    63601 caacccggcg ggcgctgaag ctgttaccat cacgcagcgt ttcgacatca taaataatcg
    63661 gtttcttact atcgcctggg cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt
    63721 ccgggacggt ctcttttgca gcatacaagg cctgacccac gacctggccg ccaaacacct
    63781 ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc tgcctcaatt ttttccagat
    63841 ttaacaatgc cagtaaattt tttagcgcct gactcatata actctccagt aacaaagctg
    63901 ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg ccactggtcc gatgggtgca
    63961 ataatctgaa ttacgggcta attacaggca gaaatgcgtg atgtgtgcca cacttattga
    64021 cgttactatt ttgttaacca ctcttccggc gaggaaagtt agcccgctgg tgcattgata
    64081 ataaggagaa atgaatgaaa ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt
    64141 tggcggcttg cgcagataaa agcgcggata ttcagacgcc agccccggct gcaaatacgt
    64201 ctatttcagc aacacaacaa cctgctatcc agcaaccgaa tgtctccggt accgtctgga
    64261 tccggcagaa agtcgcactg ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt
    64321 cgttagccga tgcaccgtca aaagtgctgg cgcagaaagc ggtgcgtacc gaaggtaaac
    64381 agtcaccatt cagctttgtt ctgccattta acccggcaga tgttcagccg aacgcgcgta
    64441 ttctgttgag tgcggcgatt accgtgaatg acaaactggt atttattacc gataccgttc
    64501 agccggtgat caacaagggc ggaactaaag ccgacctgac attggtgccg gtgcagcaaa
    64561 ccgccgtgcc ggttcaggcc agcggtggtg caacgactac cgtaccttcg acttcaccaa
    64621 ctcaggtgaa tccgtcttcg gcagttccag ctcctacgca atattaagcg acgttaaccc
    64681 tctccgcccg
//
LOCUS       DAJVOY010000005        61570 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVOY010000005 DAJVOY010000000
VERSION     DAJVOY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.005"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..353
                     /locus_tag="OQL90_000296"
     CDS             <1..353
                     /locus_tag="OQL90_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCS1367194.1"
                     /translation="SALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEV
                     QAVELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVD
                     GVDLMEAHRNALGL"
     gene            408..710
                     /locus_tag="OQL90_000297"
     CDS             408..710
                     /locus_tag="OQL90_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1367195.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            725..844
                     /locus_tag="OQL90_000298"
     CDS             725..844
                     /locus_tag="OQL90_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCS1367196.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            837..3914
                     /locus_tag="OQL90_000299"
     CDS             837..3914
                     /locus_tag="OQL90_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1367197.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            3911..4396
                     /locus_tag="OQL90_000300"
     CDS             3911..4396
                     /locus_tag="OQL90_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1367198.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            4393..5493
                     /locus_tag="OQL90_000301"
     CDS             4393..5493
                     /locus_tag="OQL90_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCS1367199.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            5584..5802
                     /locus_tag="OQL90_000302"
     CDS             5584..5802
                     /locus_tag="OQL90_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCS1367200.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(6037..7722)
                     /locus_tag="OQL90_000303"
     CDS             complement(6037..7722)
                     /locus_tag="OQL90_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1367201.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRI
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            7992..8369
                     /locus_tag="OQL90_000304"
     CDS             7992..8369
                     /locus_tag="OQL90_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1367202.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8399..8656)
                     /gene="grxA"
                     /locus_tag="OQL90_000305"
     CDS             complement(8399..8656)
                     /gene="grxA"
                     /locus_tag="OQL90_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1367203.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8816..9103
                     /locus_tag="OQL90_000306"
     CDS             8816..9103
                     /locus_tag="OQL90_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1367204.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9087..9809
                     /gene="nfsA"
                     /locus_tag="OQL90_000307"
     CDS             9087..9809
                     /gene="nfsA"
                     /locus_tag="OQL90_000307"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1367205.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            9870..10772
                     /gene="rimK"
                     /locus_tag="OQL90_000308"
     CDS             9870..10772
                     /gene="rimK"
                     /locus_tag="OQL90_000308"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1367206.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            10860..11336
                     /locus_tag="OQL90_000309"
     CDS             10860..11336
                     /locus_tag="OQL90_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1367207.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11687..12799
                     /gene="potF"
                     /locus_tag="OQL90_000310"
     CDS             11687..12799
                     /gene="potF"
                     /locus_tag="OQL90_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1367208.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            12894..14027
                     /gene="potG"
                     /locus_tag="OQL90_000311"
     CDS             12894..14027
                     /gene="potG"
                     /locus_tag="OQL90_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1367209.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14037..14990
                     /gene="potH"
                     /locus_tag="OQL90_000312"
     CDS             14037..14990
                     /gene="potH"
                     /locus_tag="OQL90_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1367210.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            14987..15832
                     /gene="potI"
                     /locus_tag="OQL90_000313"
     CDS             14987..15832
                     /gene="potI"
                     /locus_tag="OQL90_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1367211.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            15892..16380
                     /locus_tag="OQL90_000314"
     CDS             15892..16380
                     /locus_tag="OQL90_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1367212.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16421..17578
                     /gene="rlmC"
                     /locus_tag="OQL90_000315"
     CDS             16421..17578
                     /gene="rlmC"
                     /locus_tag="OQL90_000315"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1367213.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQSEAAGAASDC"
     gene            complement(17722..18453)
                     /gene="artJ"
                     /locus_tag="OQL90_000316"
     CDS             complement(17722..18453)
                     /gene="artJ"
                     /locus_tag="OQL90_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1367214.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18744..19412)
                     /gene="artM"
                     /locus_tag="OQL90_000317"
     CDS             complement(18744..19412)
                     /gene="artM"
                     /locus_tag="OQL90_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1367215.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19412..20128)
                     /gene="artQ"
                     /locus_tag="OQL90_000318"
     CDS             complement(19412..20128)
                     /gene="artQ"
                     /locus_tag="OQL90_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1367216.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20135..20866)
                     /gene="artJ"
                     /locus_tag="OQL90_000319"
     CDS             complement(20135..20866)
                     /gene="artJ"
                     /locus_tag="OQL90_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1367217.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYNSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(20884..21612)
                     /gene="artP"
                     /locus_tag="OQL90_000320"
     CDS             complement(20884..21612)
                     /gene="artP"
                     /locus_tag="OQL90_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1367218.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21830..22345)
                     /locus_tag="OQL90_000321"
     CDS             complement(21830..22345)
                     /locus_tag="OQL90_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1367219.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            22471..22794
                     /locus_tag="OQL90_000322"
     CDS             22471..22794
                     /locus_tag="OQL90_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1367220.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            22791..23621
                     /gene="amiD"
                     /locus_tag="OQL90_000323"
     CDS             22791..23621
                     /gene="amiD"
                     /locus_tag="OQL90_000323"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1367221.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(23618..24628)
                     /locus_tag="OQL90_000324"
     CDS             complement(23618..24628)
                     /locus_tag="OQL90_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1367222.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(24727..26157)
                     /locus_tag="OQL90_000325"
     CDS             complement(24727..26157)
                     /locus_tag="OQL90_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1367223.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(26168..27169)
                     /gene="ltaE"
                     /locus_tag="OQL90_000326"
     CDS             complement(26168..27169)
                     /gene="ltaE"
                     /locus_tag="OQL90_000326"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1367224.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(27206..28924)
                     /gene="poxB"
                     /locus_tag="OQL90_000327"
     CDS             complement(27206..28924)
                     /gene="poxB"
                     /locus_tag="OQL90_000327"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1367225.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(29057..30025)
                     /gene="hcr"
                     /locus_tag="OQL90_000328"
     CDS             complement(29057..30025)
                     /gene="hcr"
                     /locus_tag="OQL90_000328"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1367226.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(30037..31689)
                     /gene="hcp"
                     /locus_tag="OQL90_000329"
                     /gene_synonym="priS"
     CDS             complement(30037..31689)
                     /gene="hcp"
                     /locus_tag="OQL90_000329"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1367227.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLADKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(31833..32732)
                     /gene="lysO"
                     /locus_tag="OQL90_000330"
     CDS             complement(31833..32732)
                     /gene="lysO"
                     /locus_tag="OQL90_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1367228.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(33226..33921)
                     /gene="aqpZ"
                     /locus_tag="OQL90_000331"
     CDS             complement(33226..33921)
                     /gene="aqpZ"
                     /locus_tag="OQL90_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCS1367229.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            34347..36005
                     /locus_tag="OQL90_000332"
     CDS             34347..36005
                     /locus_tag="OQL90_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1367230.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(36002..36958)
                     /locus_tag="OQL90_000333"
     CDS             complement(36002..36958)
                     /locus_tag="OQL90_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCS1367231.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            37109..38224
                     /gene="macA"
                     /locus_tag="OQL90_000334"
     CDS             37109..38224
                     /gene="macA"
                     /locus_tag="OQL90_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1367232.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            38221..40167
                     /gene="macB"
                     /locus_tag="OQL90_000335"
     CDS             38221..40167
                     /gene="macB"
                     /locus_tag="OQL90_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1367233.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLITIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(40240..40464)
                     /gene="cspD"
                     /locus_tag="OQL90_000336"
     CDS             complement(40240..40464)
                     /gene="cspD"
                     /locus_tag="OQL90_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1367234.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            40787..41107
                     /gene="clpS"
                     /locus_tag="OQL90_000337"
     CDS             40787..41107
                     /gene="clpS"
                     /locus_tag="OQL90_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1367235.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            41138..43414
                     /gene="clpA"
                     /locus_tag="OQL90_000338"
     CDS             41138..43414
                     /gene="clpA"
                     /locus_tag="OQL90_000338"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1367236.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(43758..43845)
                     /locus_tag="OQL90_000339"
     tRNA            complement(43758..43845)
                     /locus_tag="OQL90_000339"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43809..43811),aa:Ser,seq:gga)
     gene            complement(44099..44317)
                     /gene="infA"
                     /locus_tag="OQL90_000340"
     CDS             complement(44099..44317)
                     /gene="infA"
                     /locus_tag="OQL90_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1367237.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(44602..45306)
                     /gene="aat"
                     /locus_tag="OQL90_000341"
     CDS             complement(44602..45306)
                     /gene="aat"
                     /locus_tag="OQL90_000341"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1367238.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(45348..47069)
                     /gene="cydC"
                     /locus_tag="OQL90_000342"
     CDS             complement(45348..47069)
                     /gene="cydC"
                     /locus_tag="OQL90_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1367239.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(47070..48836)
                     /gene="cydD"
                     /locus_tag="OQL90_000343"
     CDS             complement(47070..48836)
                     /gene="cydD"
                     /locus_tag="OQL90_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1367240.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(48959..49924)
                     /gene="trxB"
                     /locus_tag="OQL90_000344"
     CDS             complement(48959..49924)
                     /gene="trxB"
                     /locus_tag="OQL90_000344"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1367241.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            50469..50963
                     /gene="lrp"
                     /locus_tag="OQL90_000345"
     CDS             50469..50963
                     /gene="lrp"
                     /locus_tag="OQL90_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1367242.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            51098..55048
                     /gene="ftsK"
                     /locus_tag="OQL90_000346"
     CDS             51098..55048
                     /gene="ftsK"
                     /locus_tag="OQL90_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1367243.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQQVAPQPQYQQPQQPV
                     APQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVE
                     ARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEV
                     IPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMP
                     HLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVV
                     TDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGD
                     SMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRP
                     SVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRV
                     HGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQF
                     VTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            55203..55814
                     /gene="lolA"
                     /locus_tag="OQL90_000347"
     CDS             55203..55814
                     /gene="lolA"
                     /locus_tag="OQL90_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1367244.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            55825..57168
                     /gene="rarA"
                     /locus_tag="OQL90_000348"
                     /gene_synonym="mgsA"
     CDS             55825..57168
                     /gene="rarA"
                     /locus_tag="OQL90_000348"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1367245.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            57259..58551
                     /gene="serS"
                     /locus_tag="OQL90_000349"
     CDS             57259..58551
                     /gene="serS"
                     /locus_tag="OQL90_000349"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1367246.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            58790..61234
                     /gene="dmsA"
                     /locus_tag="OQL90_000350"
                     /pseudo
     CDS             58790..61234
                     /gene="dmsA"
                     /locus_tag="OQL90_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            61245..>61570
                     /locus_tag="OQL90_000351"
     CDS             61245..>61570
                     /locus_tag="OQL90_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1367247.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMA"
BASE COUNT        15092 a        15452 c        16611 g        14415 t
ORIGIN      
        1 acagtgcact ggatacagaa ttttccattg gtggtactga actgctgctg tttaagcaga
       61 tgggcaaagc cacggtggat ggcatccagc tgcgctttac cggctctatt cagcgtgatg
      121 ataccgggga agtgcaggcc gtggagcttg tggtgcgtgg acgtcacaaa gaagtagatt
      181 ccggcgagtg gaagacgggc gaaagcaaca ctaccaaagt gaccagtacc aacagctacg
      241 cgaagctgac catcaatggt gaggtgctct atgaagtgga cctgatcaac atggtggaaa
      301 ttgtggacgg tgtggacctg atggaagcgc accgcaacgc cctcggcctc tgatgtatct
      361 gaacggcgcg gaatgccgcg ccagaaccta atttacagga caacaaaatg agcgataagc
      421 agactgaaaa gaccattcaa ctggatactc ccatcatgcg cggtaaaaca gaaattaccg
      481 aaattgtgtt gcgtaaaccg cagtccggtg cgctgcgcgg cacacgcctg caggccatta
      541 tggatatgga tgtgaacgcg atgatgaccg tgatcccccg catctccagt ccggcactga
      601 ctgcacagga aattgcagag atggacccgg cagatctcac tgccatgtcg gttgaggttg
      661 tcactttttt gttgaagaag tcggtgcttg ccggtttacc gacagcctga cggttgacga
      721 tctggtggct gatatcgcca ccatctttca ctggccgcca tccgttactg acgttatgcc
      781 gctgaccgaa gtgctggaat ggcgggataa agcgattcag agaagcgggg ccagccatga
      841 gtgacaataa cctgcgtctg caggtgattc ttaatgcggt tgacaaactc acccgcccat
      901 ttcgatccgc gcaggccagt tcaagagaac tggctgctgc tgtcaaaaaa tcccgcgatg
      961 caataaagca gcttgatcag gccgggagca gtctggacag cttccgaaag ctgcaggcag
     1021 aaaatcagaa attaggcgac aggctgaact atgcccgcca gcgtgcaaat ttgctcagtc
     1081 aggaactggg agcgatgggg ccgccttcgc aacgtcaggt tgttgctctg ggccgtcaac
     1141 ggctggctgt tcagcgccta gaagaacgcc agaaaaagct gcagcagcag acggcgcttg
     1201 tgcgtgcaga actttatcgt gctggtattt cagctaatga tggcgccagt gcgacggccc
     1261 gcattacccg tgaaacgatg cgttataaca ggcagctttc tgagcaggaa gcgaggttac
     1321 gacgtgtcgg ggagcaacag cgaaaaatgc acgccgcccg gggggcttac gccaggcgtc
     1381 ttgaggtaag ggatcgtatt gcaggtgccg gagccaccac cacggctgca gggctggcaa
     1441 tgggcgcacc agtgatggcg gcagtaaaaa gctataccag catggaagat gccatgaaag
     1501 gtgtggcaaa gcaggtcaat ggtctgcgtg acgataatgg caaccgcact gcgcgttttt
     1561 acgaaatgca gggtgccatc aaggctgcca gcgaacagtt gccgatggaa aacggtgctg
     1621 tggacttcgc cgcactggtt gaaggtggtg cgcgcatgaa cgtcgcaaac cctgacgaca
     1681 gctgggaaga ccagaaacgt gacctgctgg ccttcgccag cacggcggca aaggcggcaa
     1741 cagcctttga actgccagcg gatgaactgt cagaaagtct ggggaaaatc gcccagctct
     1801 acaaaatccc tacccgcaat attgagcagc tcggtgatgc gctgaactat ctggatgata
     1861 acgccatgtc gaaaggggcg gacatcattg atgtcatgca acgcctgggc ggtgtggctg
     1921 accgtctgga ttatcgtaaa gcggcggcgt tgggttccac cttcctgaca ctcggcgctg
     1981 cgcctgaggt tgcagccagt gcagcaaacg cgatggtgcg tgaattgtcc attgccacca
     2041 tgcaaagcaa gagtttcttt gaagggatga atctgctgaa actcaatcca gaagtgattg
     2101 aaaagcagat gacgaaggat gcgatgggaa ctatccagcg ggtgctggag aaggtgaatg
     2161 cgctgccaca ggataagcgc ctgtctgcca tgaccatgtt gtttggtaaa gagtttggcg
     2221 atgacgcagc gaaactggca aacaacctgc cggaacttca gcgccagtta aaactgactg
     2281 cgggcaatga tgcgctcggt tccatgcaga aagaatccga catcaacaag gattcacttt
     2341 ctgctcagtg gttgctggtc aaaaccggag cacagaacac cttcagcagc ctgggggaaa
     2401 cgctgcgcca gccgctgatg gatattctgt acacggtgaa aagcgtcacg ggggcgttgc
     2461 gtcgctgggt ggaagctaac ccggaactga cgggcactct gatgaaagta gccgctgtgg
     2521 tagctgccgt taccgtgggc ctcggcacct tagcggtggc gctggctgca gtgctggggc
     2581 cgctggcagt catccgtctg ggattctctg tgctgggtat caaaacgtta ccttccgtta
     2641 cggcaacagt aacccgaacc agcaacgcgt tgtcctggct ggctggcgca ccactggcac
     2701 tgctgcgacg cgggcttgct tcatcgggca acgctgcagg tttacttact gcgccgttgt
     2761 cgtctttgcg ccgcacggca tcactgacgg gaaatgtcct gaaaactgta gcaggtgcgc
     2821 cggttgcact tttgcggtct ggattatccg gtttacgtgc ggttgctgtg atgtttatga
     2881 atccactggc ggtactacgc ggcggactgg ctgccacagg cacggtgctg cgtatgctcg
     2941 catccggtcc actggcgatg ctgcgcgttg ccctgtatgc cgtctctggt ctgttaggtg
     3001 ctctgctcag tctgataggt cttgtggtta ctgcactggc gggtgtggcg ctggttgtct
     3061 ggaaatactg gcagcccatc accgcatttc tcggtggcgt ggtggaagga ttcaaagcgg
     3121 cggcaggtcc tgtcagtgcc gcgttcgaac cgcttaagcc tgtgttccag tggattggcg
     3181 acaaagtaca ggcgctgtgg ggctggttta ctgatctgct gacgcccgtt aagtcgacct
     3241 ctgccgaact gcagagtgca gcggcaatgg ggcgacgatt cggggaggct ctggcggaag
     3301 ggctgaatat ggtcatgcat ccgctggact ccctgaaatc cggcgtttcc tggttgctgg
     3361 agaaacttgg cattgtcagt aaagaggccg caaaggcgaa actgccggaa agcgtgacgc
     3421 gtcagcaacc tgcgacggtg aatgcagacg gtaaagtgat gatgccatcg ggtggttttc
     3481 cgtcatgggg atatggcttt gcggggatgt atgacagcgg cgggtatatc ccgcgcgggc
     3541 agtttggcat cgtcggtgaa aacgggccgg aaattgttaa cggcccggca aatgtgacca
     3601 gccggagaaa tacagctgca ctggctgccg ttgttgccgg aatgatgggt gttgctgcta
     3661 cgccagcaga gcttccaccg ttgcaccctt tggcacttcc cgcgaaaggc ggtgaagcga
     3721 tggtgagtcg tgcagccact gtgccgcccg ttcaacggat tgaggcaccg atgcagatca
     3781 tcattcaaac gcagccagga caaagtgcgc aggatattgc gcgggaggtg gcccgccagc
     3841 ttgatgaacg tgaacgcagg ctgaaggcaa aagccaggag taactacagc gatcaggggg
     3901 gatacgacgc atgatgatgg tgctgggatt gtacgtgttt atgctgcgca ccgtgccgta
     3961 tcaggagctg cagtatcaac gcagctggcg acatgcggca aacagccggg taaaccgacg
     4021 cccgtcaacg cagtttctgg gaccggacaa cgacatgctg acgctttctg gtgttcttat
     4081 gccggaggta acgggcggca ggctgtcgtt gctggctctg gagcagatgg cagaacaggg
     4141 aaaagcatgg cccctgattg aaggcagcgg cacgatttac ggcatgtatg tgattgaggg
     4201 actgaatcag actaaaacgg agtttttccg cgacggtatg ccgcgccgga ttgagttcac
     4261 cctgtcgcta aaacgcgtgg atgaatccct gtccgatatg ttcggtgatc tcagtgcgca
     4321 actgaataat ctgcagggaa cggcaacatc tgccttaagc gatatcagta aaacggtggg
     4381 agggctgctg tcgtgaattt cagctctgaa ctgcttaaca aaggcaacaa aactccggca
     4441 ttcagcatca gtattgaagg cagggatatc accactgtgc tggacaaccg cctgatgggg
     4501 ctgacgctga cggataaccg gggctttgaa gcggaccagc ttgatctgga gctggacgac
     4561 gccgatggaa aaatcgtgct gccgcgccgt ggggctgtca ttacgctggc gctgggctgg
     4621 aagggacagc cgcttttccc gaaaggggca ttcacggtgg acgagattga acacactggc
     4681 gcaccggatc gcctgactat ccgggcgcga agtgctgatt ttcgtgaaac gctgaatacc
     4741 cgccgtgaaa agtcgtggca caagaccacc gttggggaag tggtgaagga aatagctgcg
     4801 cggcacaaac tgaagatggc attgggtgaa gacttgtcgg ataaactcgt ggagcatata
     4861 gaccagacta atgagagtga cggcagtttt ttgatgcggc tggcgcgcca gtacggtgct
     4921 attgcgtcgg tgaaaaatgg caatctgtta ttcatccggc aggggcaggg taaaagcgcc
     4981 agcggtaaac cactaccggt gatcactatc acacgcaagg acggcgacag tcaccgcttt
     5041 accctggcag atcgcggagc ttacacgggc gtcattgcca gctggttgca tacccgcgaa
     5101 cccgcgaaga aagaaagcac cacggtgaag cgtaagcgca ggactaagaa gcagaagaaa
     5161 gagccggaag cgaagcaggg cgattacctg gtgggtacgg atgaaaacgt gctggtactt
     5221 aatcgcactt atgccaaccg gagcaacgcc gaacgggcag cgaaaatgca gtgggaacgc
     5281 ctgcaacgcg gcgttgcatc attctcgcta caactggcgg aagggcgggc agatctctac
     5341 acagaaatgc ctgtgaaggt cagtggcttt aaacagccgc tagatgatgc ggaatggacc
     5401 attacgactc tgacgcatac tgtcagcccg gataacggtt ttacgaccag tctggagctt
     5461 gaagtgagga ttgatgattt cgaaatggaa tgattcttcg caatggagaa cttttaagtt
     5521 tgcaaaatgg aataatgcgg tatcattatt gtgaatttag caaaaatggg gagaactcga
     5581 aaaatgatga tttgcccact gtgtggaagt gccgcccata ctcgcagcag ttttcaggta
     5641 tcttcattga ccaaagagcg ttacaaccag tgccagaaca ttaactgcag ccatactttt
     5701 gttacccatg aaacttttgt tcgttcgatt gcaacgccaa aagagtcaaa tccggttcag
     5761 ccgcatccaa tgaaatcagg acaggtggcg ctctctcttt gacgctgccg ccattttgtc
     5821 gccatcgtta aaaaacagtg cttctaacat catgatttta aaaggcataa atttcagaca
     5881 acaaaaaccc atcaaccttg aaccgaaatg gcggggttga tgggctccac aaaatgggga
     5941 catcaaagaa aagcagtggc actaattaag actgatgccc tgcggaaaag ttctgcggtt
     6001 gtgcaaaaaa atttcatttt cagggcaact tcagttttat cctaatcctg gccataccat
     6061 gacgatgatt gtccctgcca gcgtcagcag gacgttggcg attgcgtagg tgcccgcata
     6121 gcccagcgca gggatgttac tgcgagctgt atcactgatg atctccattg ccggcgcgca
     6181 ggtgcgtgcg cccatcattg cgccgaacaa cagtgcgcgg ttcattcgca atacataagc
     6241 accgaacaag aaacagataa ccacgggcac aagactgaca attaatccgg caatcaacat
     6301 ctgaccgcca atcgcgccca ggccgttatt aataccgcta ccggcgctca gaccaacgcc
     6361 tgccataaac accatcaagc cgaactcttt caccatgctt aatgcacctt gcggaatgta
     6421 accgaaggtc gggtggttag cacgcataaa gcccagcata attccggcga ataacaaccc
     6481 ggcagcgttc cccatgccga aactgaatgt gctgaactgg aaggtgatca tcccgatcat
     6541 cagcccaata acaaagaagg cgcaaaatgc cagcaggtca gtgacctggc tgtgaatcga
     6601 gataaagccg atgcgatcgg cgatggtttt tacgcgacgg gcatcaccgc tgacttgtaa
     6661 aacgtcacct ttgttaagca cgacgttgtc atctatcggc atctcaatct ggctacgaat
     6721 gatgcggtta aggaagcaac cgtgatcggt caacttcagt tgtgcgagac gtttacctac
     6781 agcgttatgg tttttaacga ccacttcttc agtgacgata cgcatgtcga gaaggtcacg
     6841 atcgaaaact tctttaccgt tacggaagct gggatcgagt cgggcatggg cgtcgggata
     6901 gcctaccaac gctatttcat cgcccatttg tagcacggca tcaccgtctg gatttgccag
     6961 aatcccgtta cgtcgaatac gttcaatgta gcagccggtt tgtcgataaa tacccagttc
     7021 acgcagattt ttgccgtcgg tccaggccac cagctccggg ccgacgcgat aggcgcggat
     7081 caccggtaaa taaaccttac ggttggcatc agtgtccagg ccacgttcgc gggcgatttg
     7141 ctgggcgctg gtctgtaagt cctgatgctg caatttcggc aagtaacgcg caccaacaat
     7201 caaactcacc agaccgatta aataggttaa ggcatacccg aggctcagat tatccagtgc
     7261 cagtgagagc tgcctgcttt ccatgccgga atgacgcagt gtatcgccag caccgaccag
     7321 aaccggtgtc gacgtcatag agcctgctaa cataccggcc gtcaggccaa tatcccagcc
     7381 aaacagctta cctaacccta aggcgatcac cagcgcactg ccaaccatca ccagtgctaa
     7441 cattaggtaa tttttcccat cgcgaaaaaa aatggaaaaa aagttcggtc cggcttcgac
     7501 cccgacgcag aaaataaaca gcataaagcc aagattaagc gcatcggtgt taatgctgaa
     7561 atgttgttgg cctaataaca gcgatacgac taaaacgcca atggaattac ccagttggat
     7621 cgaaccaagt cgtaactttc cgagacatag cccaagcgcg aggaccacaa ataataacag
     7681 aatgtaattc ccatttaaca attcggcgac gtttatattc acggaggcta acttcttgtt
     7741 tactagtaag ctgttgaaag aaatggtaat ttacgataat gttttttacc agaattcagg
     7801 gcgcagattc attcagcgca cctaaacgat agtaaagtaa caatatattt tactagtgta
     7861 atcacattag gtatcaacgg ctatatgaat tgcgttggcc tatattagca tggaatgcga
     7921 agcggcttta tcttactgaa cgccacactg gcgaaaaatg tgttcgatag acgcagtgtc
     7981 aggaggaacg agtgaaacat aaacaacgtt gggcgggggc aatctgctgt tttgtcctct
     8041 tcattgtggt gtgccttttt ctggcgacgc acatgaaagg cgcttttcgg gctgccgggc
     8101 atcctgaaat cggcttgctg tttttcattc ttcctggagc agttgccagt ttcttttcac
     8161 agcgtagaga agtcctgaaa cctctatttg gcgcaatgct ggcggcaccc tgttcaatgc
     8221 tcattatgcg gctgtttttt tcaccgacgc gctcattctg gcaagagctg gcatggttac
     8281 taagcgcggt gttctggtgt gcgctggggg cactgtgttt cttatttatc agtagtttgt
     8341 ttaaaccaca gcacagaaaa aatcagtaaa gccctcaacg cgagggcttg tcagacgatc
     8401 aggcgtccag attttctttc acccatgcag caaaatcggt atagccgccg atatgttgct
     8461 gatcgacaaa aatctgcggc acggtttcta cgggtttacc tgccttttgt tgtagatctt
     8521 ctttagtgat cccttccgca cgaatatcta catactgata ctgaaaatca tcgcgttcat
     8581 tgctcaattt ctcagccaga tcttttgcac gcacacagta agggcaaccc gaacgaccaa
     8641 aaataacggt ttgcattatt tctctcctca tagatttatg cctgtaatga tcacgctaaa
     8701 atgtattcgc tgaaagtagg tttaacctgt tgcattaatt gctaaaagct ataactgtta
     8761 aacacaatac agtgaaaagt tttagactga aggctcactt tgcagaggga agcgtatgcg
     8821 cgcgatcggt aaattgccta aaggcgtgtt gatactggaa tttatcggaa tgatgctact
     8881 ggcggtggcg ctgctgtcgg taagcgactc cctgtcgctg cctgagccat tttctcggcc
     8941 agaagtgcag attctgatga tttttctcgg tgttttgctc atgcttcccg ctgcggtggt
     9001 ggttattctt caggtggcaa aacgtcttgc cccacagctg atgaaccgtc caccgcaata
     9061 ttcacgttca gaaagagaaa aagataatga cgccaaccat tgaacttatt tgtggccatc
     9121 gctccattcg ccatttcact gatgaaccca tttccgaagc gcagtgtgag gcgattatta
     9181 acagcgcccg tgcgacgtcc agttccagtt ttttgcagtg tagtagcatt attcgcatta
     9241 ccgacaaagc attacgtgaa gaactggtga cgctgaccgg cgggcaaaaa cacgtagcgc
     9301 aagcggcgga gttctgggtg ttctgtgccg actttaaccg ccatttacag atctgtccgg
     9361 atgctcagct cggcctggcg gaacaactgt tgctcggtgt cgttgatacg gcaatgatgg
     9421 cgcagaatgc attaaccgca gcggaatcgc tgggattggg cggggtatat atcggcggcc
     9481 tgcgcaataa tattgaagcg gtgacgaaac tgctgaaatt accgcagcat gttctgccgc
     9541 tgtttgggct gtgccttggc tggcctgcgg ataatccgga tctcaagccg cgtttaccgg
     9601 cctccatttt ggtgcatgaa aacagctatc aaccgctgga taaagacgca ctggcgcagt
     9661 atgacgagca actggcggaa tattacctca cccgtggcag caataatcgc cgggatacct
     9721 ggagcgatca tatccgccga acaatcatta aagaaagccg cccatttatt ctggattatt
     9781 tgcacaaaca gggttgggcg acgcgctaaa accgccacgt cgatgtatga tacgcgggct
     9841 tttgaccagg tctgacagag aggtgcaggg tgaaaattgc catattgtcc cgggatggaa
     9901 cgctctattc gtgtaagcgg ctgcgtgaag ccgctataca gcgcggtcac ctggttgaaa
     9961 ttcttgatcc gctttcttgc tacatgaaca taaatcctgc ggcgtcttct attcactaca
    10021 aaggccgcaa gttaccccat tttgacgcag tgatcccgcg tattggcacc gccattacct
    10081 tttatgggac ggcggcactg cgccagttcg agatgctggg gagctatccg ctcaatgagt
    10141 cggtcgccat tgcccgggcg cgtgacaaat tgcgttccat gcaactgctg gcgcgtcagg
    10201 gcatcgacct gcctgtcacg ggcattgcgc attcgccgga tgataccagc gatttaatcg
    10261 acatggtcgg tggtgcgccg ctggtggtca agttggttga aggcacgcag ggaattggcg
    10321 tcgtgctggc ggagacgcgt caggcggcgg aaagcgtgat tgacgctttc cgcggtctga
    10381 acgcgcatat tctggtgcag gaatatatca aagaggcgca agggtgcgat atccgctgtc
    10441 tggttgttgg cgatgaagtg gtcgctgcga ttgaacggcg ggcgaaagag ggcgattttc
    10501 gttccaattt gcatcgtggc ggcgcggcaa gcgtcgccag tatcacacca caggagcgtg
    10561 aaatcgcgat aaaagccgcg cgaacgatgg cgctggacgt tgctggtgtg gatattctgc
    10621 gtgctaatcg cgggccgttg gtgatggaag tgaatgcgtc gccggggctg gaaggaatag
    10681 aaaaaaccac tggtatcgac atcgcgggta aaatgatccg ctggatcgaa cgctacgcta
    10741 cgacagaata ttgcctgaaa acgggtggtt agtcgcaatc acattactga tcatggtttt
    10801 gcctgcgctt tttgcgtaag ctgtgccggt ctttttatcg aaagaggttg tacaaaatta
    10861 tgacatcgct ggtcgttcct ggtctggata cgctgcgtca atggctcgat gacctgggga
    10921 tgagtttttt tgaatgtgat aactgtcagg ctctgcatct gccccatatg cagaatttcg
    10981 acggtgtctt tgatgccaaa atcgatctga tcgataacac gatcctgttt tctgccatgg
    11041 cggaagtccg accttcagcc gtattgccgc tggcggcgga tttatctgcc atcaatgcca
    11101 gttcgctgac cgtgaaagca tttcttgata tgcaggatga taatctgcca aagctggtgg
    11161 tttgccagtc tttatccgtt atgcagggcg taacctacga gcagtttgca tggttcgtgc
    11221 gtcagagcga agagcagatt tcgatggtca ttcttgaagc taatgcccat caactgctgt
    11281 taccgactga cgatgaaggg caaaacaacg ttaccgaaaa ctatttcctc cactgataac
    11341 tcctttcgag cacgcagtcg ctggtgcagt gactgcgcac tgcaaaatta tctgctgttt
    11401 ttaacctttt cttaaagatt atttcacttc tcttgtgccg atttggcttt atcacataga
    11461 gcaaatacgc ataacaattt gttaaatacc gttttttaat ccgagctata gtctcaaacc
    11521 ctggctaaag ttattcttgc gatgctttta tatagcgagc agtgctggcc gggagagagt
    11581 tatcttttct tacaccgcgc cgataaaaaa tatgcacgtt tattgcatat cttttagtat
    11641 gacaactttt gttcgtttgt taacgaactt tcagaaggaa agagatatga ccgccttaaa
    11701 taaaaaatgg ctttcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct
    11761 cgcggctgaa caaaaaacac tccacattta taactggtct gattatatcg cccctgacac
    11821 ggtggccaat tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa
    11881 cgaagtactg gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc
    11941 tgccagcttt cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa
    12001 attgccggag tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc
    12061 cgacaataaa tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga
    12121 taaagttaaa gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa
    12181 acctgaaaat ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga
    12241 agtttttgct accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga
    12301 ttacaccgga ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca
    12361 ttcatctcaa tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc
    12421 aggtgatgtc tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc
    12481 gttctcgatt ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga
    12541 tgccaaaaac aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt
    12601 agcgcatatt tccgaccatg tgttctatgc caacgccaat aaagcagcca cgccgctggt
    12661 gagtgcggaa gtccgtgaga acccgggtat ttatcctccg gcggacgttc gtgcgaagct
    12721 attcactctg aaagtacagg atccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa
    12781 agtgaagagc ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg
    12841 gcaattcgca ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg
    12901 acgctatccc tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc
    12961 gcaacctcac caaatcctac gatggtcagc atgcggtcga tgatgtcagc ctgaccatct
    13021 acaaaggtga aatcttcgcg ctgctgggcg cttccggctg tggcaagtcc acgctgctgc
    13081 gtatgctggc aggttttgaa caaccttctg ccgggcagat aatgctcgat ggcgtcgatt
    13141 tgtcacaggt gccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt
    13201 ttccgcatat gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga
    13261 aagcggaaat tgccagtcgg gtccatgaga tgctcggcct ggtgcacatg caggagttcg
    13321 ccaaacgcaa accgcatcag ctttccggtg gtcagcgaca gcgtgtggcg ctggcccgaa
    13381 gccttgccaa gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa
    13441 agctgcgtga ccggatgcag cttgaagtgg tggatattct ggagcgtgtc ggcgtgacct
    13501 gtgtgatggt cactcacgat caggaagagg cgatgacgat ggcgggacgt atcgccatta
    13561 tgaatcgcgg gaaatttgtc cagattggcg agccagaaga gatctacgaa catccaacca
    13621 cccgctacag tgctgaattt atcggttcgg ttaatgtctt tgaaggtgta ctcaaagagc
    13681 gtcatgagga tgggcttgtg ctttattcgc ctgggctggt gcatccactg aaagtcgacg
    13741 cggatgcctc ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca
    13801 tgctttgcga agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca
    13861 ttgcctatct cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta
    13921 gcgcccagct acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag
    13981 tgcgtttgtg ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga
    14041 atacacttga acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc
    14101 agctgcaaat gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct
    14161 tgctgtttct gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg
    14221 ctattccacc ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta
    14281 atctcggtaa ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt
    14341 cgctacaggt ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt
    14401 gggcggtggc gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc
    14461 cgtcgtggac ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg
    14521 gtgtgctgaa taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc
    14581 ataccaatct ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc
    14641 cgatttatac cgcgttgatt cgtattgatt attcgctagt ggaagcagcg ctggatctcg
    14701 gtgcacgacc gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta
    14761 ttgccggatc gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc
    14821 tcggtggccc ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc
    14881 gcgactggcc ggtggccgcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga
    14941 taatgtggtt ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc
    15001 ggtagttcgt tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc
    15061 gccaatgctg atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc
    15121 cggctggtca acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt
    15181 tggtttaagc ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat
    15241 tgcggcggtg gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat
    15301 gatcacagcg ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt
    15361 cgtcgcgctt gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct
    15421 ggcgcatgtc actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga
    15481 actggatcgc tcgatagaag aagcagcgat ggatctcggt gcgacgccgc tgaaagtgtt
    15541 tttcgtcatt acgctaccga tgatcatgcc tgcgatcatt tctggctggt tactggcttt
    15601 tactttgtcg cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac
    15661 gttaccgatg ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct
    15721 tgcaacgtta attctcggtg cggtcggaat tgtcggattt atcgcctggt atctgatggc
    15781 tcgcgcagaa aaacagcgga tacgcgatat ccagcgtgca agacatggct gaagacacta
    15841 aaatttgcca acctggctac ataatgccgc gcatgccgcg gcattgtttt catggaagac
    15901 gaaacgttgg gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg
    15961 ctggtgcagg tagcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt
    16021 ttcaatacgc tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg
    16081 agcttaacgc tggtattttt aagcagtctg gtgctggttt ttatcgagat ctggtgtgcg
    16141 ttttcactgg tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc
    16201 gccgcaagtt acttgtgggc ggcttcgctg gggtatggtt atccggagct gttcagcatt
    16261 cccggtgaat caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg
    16321 ctcatcctga tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa
    16381 tgtgtataat cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga
    16441 cgcgggtcgc tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc
    16501 taaaaccgcc gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc
    16561 ggtgtcaggc ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt
    16621 tgaaaaacca ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg
    16681 cccgctttat cctgcctctt ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg
    16741 tgcggggtta acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct
    16801 gactgaaagc cagagcgatg gcggcatgat gctgcgcttt gtactgcgtt ctgaaaccaa
    16861 actggcacaa ctgcgtaagg cgctgccgtg gttacaggaa caactaccgc aactgaaagt
    16921 tattaccgtc aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta
    16981 cctgactgaa caacaggcgc tggcagagcg ttttaatgac gtaccgctgt ggatccgtcc
    17041 gcaaagtttc ttccagacta atccggcggt cgccagccag ctgtacgcta ccgcgcgcga
    17101 ctgggtacgg cagctgccag ttaaacatat gtgggatctg ttctgcggtg tggggggctt
    17161 tggtttacac tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc
    17221 cattgcctgt gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc
    17281 gctggactcc actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa
    17341 cccgccgcgc cgcggcattg gtaaaccgct gtgtgattat ctctcaacga tggcaccgcg
    17401 ttttatcatc tactccagct gtaacgccca aactatggcg aaagatatcc gcgaactgcc
    17461 tgggtttcgc atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga
    17521 agtgctgacg ctgctggtga agcaatctga agccgcaggt gcggcttcag attgctgatc
    17581 aggtgcgctt tgttcatgcc ggatgcggcg tgtacgcctt atccggccta caaaatcatg
    17641 ctaaatcaat acattgcaga gttgatgtgg gccagataag cgcagcgcat caggcaatct
    17701 tacttttgtc atcagtctca attactgcgg gaaccactgg tcattgattt tctgatatgt
    17761 accgtcagct ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc
    17821 cggacgtaca gcgatgccca gaccggtgcc gaagtattgc ggatcggtca ctttttcagt
    17881 agcaacgccc agttgcggat tggttttcag ccattcgttt accaccgctg tgtcaccaaa
    17941 taccccatca atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga
    18001 gacagttttc acttcggggt gctgatcctg aatatatttc tggtgcgtgg tgccgttttc
    18061 catcccaata cgtttgcctt tcagatcggc aaacgttttg taggtatctt ttttggcaat
    18121 cacgacggct gagttttcat agtagggcgt ggtaaacgat acctgcttgc tacgctcggg
    18181 cgtgatatcc ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag
    18241 gctgtcgaac gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt
    18301 tgccagatcg atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg
    18361 ataggtggct gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga
    18421 agcaagtaaa gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat
    18481 gatttttggc cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat
    18541 aaatatgatt aaataattta aatgaaataa aaaacgacgg ataacttagt gagttgtccg
    18601 tcttcattat aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg
    18661 cacaggcacc gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc
    18721 cccggcgaat gtacgcagct acattaattc cgccgttcga atgccagtgc tttgcgctcg
    18781 atcagacgca tcatcagcgt cagcaggccg ttgacgacca ggtaaataat ccctgccgca
    18841 ccgaacacca ttacatcgta ggtgcgtccg tacaacaact ggctgtatcc catcacttcc
    18901 atcagcgtaa tggtgtaagc cagagaggta cttttgaaca ccagcaccac ttcgttggaa
    18961 taagaagaga gcgagcgttt aaaggcatac ggcagcagga tcgccagcgt atcttttttg
    19021 ctcattccca gggcgctaca ggactgccac tgaccttctg ggatcgcacg aattgcaccg
    19081 taaaacagct gcgtggtata cgccgcactg ttcagcgata acgcaatcag cgcacataac
    19141 cacggttctg acaacaaatg ccacagtgcc ggatactcct gcaaagtcgg aaactggccc
    19201 ggcccgtaat aaatcaggaa gatctgcacc agcagcggcg taccggtaaa cagcgtgata
    19261 taaccccgca ccagccacac cagcaccggc gttttcagcg tcaggatgat ggtaaaaatc
    19321 aatgccagaa tcagtgccac aatcagcgag gcaacggtta gcgtcaggct ggtgtgtagc
    19381 cctttcatca gttcgggtaa atactcaaac attagctggg cctccgctca aaacgtgtcg
    19441 cgcgcaggtc aatgcgtttg agaatgtact gactgagcag ggtgatcacc aggtaaatcg
    19501 ccgccgccac aatataccag gtaaatggtt cctgggtacg agtagcgatg ctttttgttt
    19561 gcagcattaa atcattcaca ctaatcaaac tgaccagcgc ggtatctttc agcagcacca
    19621 gccactggtt accgaggcca ggcagcgcat gacgccacat ctgcggcatc accagacgga
    19681 aaaagatagc cgatttcgac agccccagcg cctgaccaga ctcccactga cccaccggca
    19741 ccgctttcaa cgcgccccga agcgtttgcg aggcataggc ggcatacagc agtgacagag
    19801 cgatgacacc acaaaggaac gggctaacgt cgaagttctc aatgtccatc tgcactggga
    19861 tctgcacgaa cccaagattg atagtgaagc catccgaaag cgtcagcagc agctgcgagg
    19921 agccaaaata gataaacagc accaccagaa tttctggcag gccacgcaga atggttacca
    19981 gcgctgaacc tgcccacgcg acaggacgcc atttttccga ctcccatacc gcaaagaaca
    20041 tcgccagcgc cagcccgaca atcaatgcac aaacggcaag gccgacggtc atcccggcgg
    20101 cgcttgctaa aggaaaaaat tcattcatca ggaattactt ctggaaccat ttgttgtaga
    20161 tggtttcgta agtgccatct ttcttcactt tttccagcgc agtgttgagt ttctgctgca
    20221 gctcagtgtt gccctgacgt accgcgatgc cgaggccagt gccgaagtaa tctttatcgg
    20281 tcactttgtc gcccaccgcc gccagtttcg ggttatcttt cagccactca gtgaccactg
    20341 cggtgtcacc gaagacgccg tcgatacgcc cgttttgcag atccagtttt gcgttctggt
    20401 agctgttata cggaacggta gtgatttccg ggtgcttatc cataatgaat ttctggtgtg
    20461 tcgtcccgtt ctgtacgccg acttttttgc ctttcagctg atcaacactg gtgtatttgc
    20521 cttgctgacc cacaaacagg gcagagttgt catagtacgg ggtggtaaac agcacctgct
    20581 tttcacgctc cggagtgata tccatgcccg ccatcacggc ttctacgcga cggaatttca
    20641 ggcttgggat caggctgtca aacgcctggt tagagaaagt gcaggttgca tcaatctctt
    20701 tacacagcgc ttgtgccagg tcgacgtcaa aaccaacgat ctggttgttt gcatcaatcg
    20761 attcaaacgg aggataggat gcttcggtag caaaacgaat ggtttcggca gctgtggcgg
    20821 aaagactaaa acctgcaatt aacgcggcaa tcagaacttt tttcattgtt gttatcccga
    20881 atcttagtga gagagatagt ttttaaatgc ttcggtttgc ggctcggtaa agcagctcgc
    20941 gtcgccttgt tctacgatat gaccattttc catatacacc actcggctgg cggttttacg
    21001 cgccacttca acttcgtggg tgacgatcac ctgggtaata ttcgtttctg ccagctcacg
    21061 aatgatgctg acgatttgtg ccgtaatttc cgggtccagt gcggcggtcg gttcatcgaa
    21121 cagcagtacc tgcggttcca tcatcaacgc acgggcaata gcaacacgct gctgctgacc
    21181 accagaaaga tgcagcgggt aacgatcgct ataaggtttg agacgcagac gttccagcag
    21241 tttttctgca cgggccagcg cctgatcttt actcaacccc agtacacggc agggcgcttc
    21301 aatcaggttt tgctgcacgg tcagatgcgg ccacaggttg tattgctgaa acaccatgcc
    21361 aacgttacga cgcaaatcgc gaatcgcttt gtcagagggt gttttggtga aatcgaaatg
    21421 gttgcctgca atgttgagcg taccggagcg cggcatctca agcagattga gtacacgcag
    21481 cagcgagctt ttacccgcgc cgctggggcc aagtaacacc agcgtttcgc cctgtgggca
    21541 atccagcgtg atatcgaaca gcgcctgatg cgcgccgtag aagcaattaa tgccgtttaa
    21601 ttgaatactc attgacactc gtatactggc agtctgatag ctattgaggt cgaagatagt
    21661 acctttgaca gaataattat gcaatatttc tgctttaaaa gttaaaagca aagcgcatta
    21721 ttcaataaac atagcacaaa ataacggggg cggtggtcgg cgagcataaa tgtcggcatt
    21781 cctcacgaaa tgccggacaa tttacggggt ttattggttg atcaaggcgt tagcgattct
    21841 cgatggactg acggagcgta cccgccgtgg catgaacgct gccgcctaag tagcgtacat
    21901 cgtcgatcac ccaacactgg ccttcctgaa tcattaacac ttcatcctgc cagctctgat
    21961 caccctgttt tagatccaca cgcagaggaa tatttcgcgc atcacgattc gggattgttg
    22021 aggcactggc aacacgggcg ctctctggca aggttgtgcg actggagaat ggatcgttgg
    22081 tcagtagttc ccgatggtta ttatcccggg aggcatcgct aagcagtgtc gccagtttgt
    22141 cgctcagata agggcgcaag gcggtgatgt cgttgctgcg gtgcaaaatg cggtagtcat
    22201 aaaattgctg ggccacgtta cccgggcctc cttcaacgca aggaccactg cgtgtgccgt
    22261 tatctttata agctggagtg actgtggtgc aggcactgag gagcagtgcg caggggataa
    22321 gcattgtcaa tttgctgtag cgcataatga tttccttata agcgatcgct ctgaaagcgt
    22381 tctacgataa taatgatatc ctttcaataa tagcgtatca gtctgataat gcttttgaga
    22441 tcgaaggctt agcaaacaag gagatcgatc atgcaatttt ctacaacccc aactctggaa
    22501 ggccagacca tcgttgaata ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat
    22561 attttccgtg atttctttgc cggtatccgc gatatcgttg gcggacgttc cggtgcgtat
    22621 gaaaaagaac tgcgtaaagc acgggagatc gcctttgagg aattaggctc ccaggcgcgg
    22681 gcgctggggg ccgatgccgt cgtcggtatt gatatcgact acgaaacggt cgggcaaaac
    22741 ggcagtatgc tgatggttag cgtcagcggt acggcggtga aaacgcgtcg atgagaagag
    22801 tcttctggct ggtcgctgcc gctctgttat tggcagggtg tgcaggcgaa aaaggcattg
    22861 tcgagaaaga gggatatcag cttgataccc gacgccaggc gcaggcggcg tatccgcgca
    22921 ttaaagtgct ggtgatccac tacaccgcag atgattttga tagctcgctg gcgacactga
    22981 ccgataagca ggtcagctcg cattatctgg tccctgcggt accaccgcga tacaacggta
    23041 aaccgcgcat ctggcaactg gtgccggaac aagaactggc ctggcatgcg gggattagcg
    23101 cctggcgcgg ggcaacgcgc cttaacgaca cctctattgg cattgagctg gaaaaccgtg
    23161 gctggcaaaa atcggccgga gtgaaatatt ttgccccgtt tgaaccggca cagattcagg
    23221 cgcttattcc actggcgaaa gatattattg cccgttatca catcaagccg gaaaacgtag
    23281 tggcacatgc ggatatcgca ccgcagcgca aagacgatcc ggggccatta tttccctggc
    23341 agcaactggc gcagcagggg attggtgcct ggccggatgc gcagcgggtt aacttttacc
    23401 ttgccgggcg cgcgccgcac acaccggtag ataccgcgtc attgctggag cttttggcgc
    23461 gctacggtta tgacgttaaa cctgatatga caccgcgcga gcagcggcgc gtgattatgg
    23521 cattccagat gcatttccgc ccgacgttat ataacggcga agcggatgca gaaactcagg
    23581 cgattgccga agcattgctg gagaaatacg ggcaggatta gcgcggcagt tttccgtggt
    23641 cgcgtagcca ggcggcagtt ttctcgatac cttcatccag ggtgatgacc ggctgataac
    23701 ctaactcttc ctgcgcacgc gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc
    23761 cgtagtgggt cagcggcggc tcttttgctg acttgcggaa acgctccatg ctgcgggcga
    23821 tcatatccag catcgggtag gggacggaac gaatacgaca gtcaatattc aactcgtcga
    23881 tcagcttctg cacgatgctg cacagtgtgc gatgctcgcc gttggtgatg ttgtacacac
    23941 gcccggaagg tagcttatcg caggcttcct ggcttgccag ccacattgcg tgcacggcat
    24001 tttcatagta ggtcatatcg accagcgcac tgccgccatg cggtaacaga atactgccgt
    24061 agtggtgcat catatgcgcc agacggggaa taaagacttt atcgtgcggt ccgaacagac
    24121 tttgtgggcg cagaatagta aagcgcgttt gtggattcgc ctgcgaaaac atattgatca
    24181 cttcttcgct ggctgctttg ctgcgggcaa actcgttggc gaagcggtga gggcgaaaat
    24241 cttctttaat atcgcgatgg tggtgataat cgaagtacag ggagggggaa gagatatgaa
    24301 taaagttacg cacaccccag gcgacagccc attcacccaa gcggcgagtg gcgcgaacgt
    24361 tagccagatc gaaagcctgt tgtgtccccc agggtgaggt aaagctggag cagtgccaca
    24421 gcgtatcaat gcccgcgagc atcactttag cttgtgatga aaccagctcg gtcagatccg
    24481 ccggaacaaa ctctgcgccc attttttcca gcaatttgcc cattgcctcg ttgcgaccgg
    24541 tcgctcgcac gctgatgcct ttctggcata aaaactctac cgcgtttcga cctaagccgc
    24601 tggtggcgcc ggtaaccagt accttcatat caatccactg ttgttgagaa aataacgtgc
    24661 gcattcttcc gtgatttccc ccatgatgca atgggaaaca tgaaagaata acgcaggttt
    24721 tgtcgattaa tctgtgcttt gttctgccag tctggcgatt tgttttgcca ttccgcgaaa
    24781 aataaacaga tgcgcgggga tcatcaataa ccagtaaaac agccccggca taccgtgcgg
    24841 atgccagaaa gcgcggacat cgatagtacg atagtcgcct ttatcttcca ggctaaaaca
    24901 cagtcgtccc agccccggcg ctttcatgcc aaataacaac gtaagttgtt tttccggttc
    24961 aacgacaatc actttccagc tatctaccgc atcgccagtc tgtaaatatt cgcgctccgg
    25021 gcggcctttc gccagcttat gaccgatcgc gcggtccatc aacgcccgtg tctgccacaa
    25081 aatattgcca aagaaataac gctctttacc gccgatttgg ttcactacct gccataaagc
    25141 agcaaggctg gcggacgttt taacggtaaa ccccgcctgt ttggcaaaat aaccgtactc
    25201 cggtcgccag cgggcaaagg cctgagcgtc gtagccccag tcgctggagt tgaccagttt
    25261 ttcctcctct ttcaacgtgc tacgtaccgc gtcatcgaaa gcgatcagcc gttgtgggat
    25321 gagtgcacgt agcgcggtat catccgccag cagatcgtgt ttcagcccct gaatcaacgc
    25381 cctggcggtg gtgggcggta cggaagtaat cacattgaga aaccacaccg aaatccagcg
    25441 ggtggggagg gggatgggga tcaaccagcg gcgcttgccg ctcaccgcca taaaatgttc
    25501 aaactgttgc tgataactga gcacctctgg tccggcggct tcgaagatgc ggtgttcgct
    25561 ggccggatga tctaacaacg ccaccagata gtgcagcaag ttttccagcg cgatgggcgt
    25621 ggtgcgtgaa cgtacccagc gtggcggcgt taacactggc aggttgtaga ccatatcgcg
    25681 catgacttcg aacgccgctg aacctgcgcc aacgataatt ccggctcgaa gttcggtcac
    25741 aggtacattc gcttcacgaa gaatgtccgc cgtagcctga cgagcacgca gatgatccga
    25801 ctgctcatgt ggcggggcct gcaacgaact gagaaagatt aattgcttaa ctggtacttc
    25861 acgtagcgca tcgcggacgt tgagagccac ctggcgctcc tgagcgataa aatcgccgcc
    25921 ttcgcccatg ctgtgcacca gaaaatagac ggtatcgata tcctgcaaca gggccggaag
    25981 gttatccggc cagctgagat cgactttatg gcaactgacg tttgccagtt gcagctttgc
    26041 aagcctgtcg acatgacgtg ccgccgccag gatctgatgc ccttgctggc tgagtgtgcg
    26101 caccagatgc tgaccaatgt agccactggc accgagaact aaaatgcgtt gcggcacgtc
    26161 tctctcctta acgcgccagg aatgcgcgcc agtgggcggc gacttccgcc agttgttcgc
    26221 gcgagacgtc aagatgcgtc accaggcgga caatcggcga ggcgttaatc agcacgtttc
    26281 tcactttcat gtattcgcct aacgcggcag cattttcttc cccgacgcga acaaacagca
    26341 tattagtgtc ctgacgcatc acatccgcgc ctgcttcacg cagttgctcc gccatccagg
    26401 cggcgttgtc gtgatcttcc tgcaaccgtg cgacgttatt tttcagggca tacatcccgg
    26461 cggcagccag aatcccggac tgacgcatcc cgccaccggc cattttccgc cagcggatgg
    26521 cacgtttaat gtaatcacga ttaccgacga gtaatgaacc gacaggcgtc ccaagacctt
    26581 tcgacaggca aatggtgaac gaatcacaat attgcgtgat ctctttcagt tcgcagccgt
    26641 aagccaccac ggcattaaag atgcgcgcac cgtcaacatg cagcgccaga ttgcgctcgc
    26701 gggtaaattc ccatgcttct ttcaggtatt cacgcggcag cactttgccg ttgtgggtgt
    26761 tttccagact gagtaatttg gtgcgggcga aatggatatc gtcgggtttg attttcatcg
    26821 ccactttatc cagcggtagc gtgccgtcgg cagccgcgtc gatgggttgc ggctgaatgc
    26881 tgcccagcac cgctgcgcca ccggcttcaa acagatagtt atgcgcggcc tgaccgacaa
    26941 tatactcttc gccacgttcg cagtgactga gcagagcgac caggttggcc tgagtgccgg
    27001 taggcagaaa aatggcggct tctttaccgg aaagctctgc tgcatagtcc tgcagagcat
    27061 taacggtagg gtcgtctccg taaacgtcgt ccccaaccgg ggcggccatc atcgcttcga
    27121 gcatggcgcg gctcggtcgg gtaacggtat cactgcgtaa atcaatcatg gcatgtcctt
    27181 attatgacgg gaaatgccac cctttttacc ttagccagtt cgttttcgcc agttcgatca
    27241 cttcatcgcc gcgtccgctg atgattgcgc gcaacatata caagctaaat cctttggcct
    27301 gttcgagttt gatctgcggt ggaatggcta actcttcttt ggcgaccacc acatccacca
    27361 gcaccggacc gtcgatggag aaggcgcgtt gcagggcttc atcaacttca gacgcttttt
    27421 ctacacggat acccgtaatg ccgcacgctt cggcaatgcg ggcaaagttt gtgtcgtgta
    27481 gttcggtgcc gtcagtcaaa tagccaccag ctttcatctc catcgccaca aagcccagca
    27541 cgctgttgtt aaagacgaca attttcactg gcagtttcat ctgcactact gagaggaaat
    27601 cgcccatcaa catgctaaaa ccgccatcgc cgcacatggc gaccacctga cgttctggct
    27661 ctgtcgcctg cgcacccagc gcctgcggca tggcgttagc catcgaaccg tggttaaacg
    27721 aacctaacag gcgacgcttg ccgttcattt ttagataacg tgccgcccac accgttggcg
    27781 taccaacgtc acaggtgaaa atagcgtcat cggcggcaaa atgactaatt tgctgcgcca
    27841 gatattgcgg gtgaatggct ttctcgctcg gtttagctaa atcgtccagc cctttgcggg
    27901 cgtcgcggta atcttccagc gctttatcca gaaacttgcg atcggctttt tcttccacca
    27961 atggaagcaa tgcacgcaga gtcgacttga tatcgccgac cagtgccata tccaccttgc
    28021 tgtgagcgcc gatgctggct gggttgatat caatctgaat gattttggca tcggtcgggt
    28081 agaaggcgcg gtagggaaat tgcgtgccga gtagcactaa cgtgtcggcg ttcatcatgg
    28141 tatggaaacc tgacgagaag ccgattaacc cggtcattcc aacatcatac ggattatcgt
    28201 attcgacatg ttctttaccg cgcagggcat gaacaatagg cgctttaatt ttcccggcaa
    28261 actcaactaa ctctttatgc gcccccgcgc agccgctgcc acacatcagg gcgatattgc
    28321 tggaataacg cagcagttgc gccagtttgc gtaactcttc ttcttccggc gtcacgactg
    28381 gttgtggcgc atgataccag tgcgtggttg ccccttctgg cgcaggtttt aacgccacgt
    28441 cgcctggtaa cacgacaacc gaaacgccac ggttaagcac cgctttgcgc atggcaatcg
    28501 ccagtacttg tgggatctgc tccgggctgg aaaccagctc gcaatagtga ctacattcgc
    28561 ggaatagctc ttgtgggtgg gtttcctgga aatagccgct gccaatttcg ctggagggaa
    28621 tatgagcggc aatcgccagt accggaacgt gattgcggtg gcaatcaaac aggccgttga
    28681 ttaagtgcag gttgccgggg ccgcacgatc cggcgcagac cgccagttct ccgctaagtt
    28741 gtgcttcagc gccagcggca aaggccgcca cttcttcgtg gcgggtggac atccactcga
    28801 tggtgcccat gcgattaaga ctgtcactaa gaccattcag agagtcgcct gtgactcccc
    28861 agatgcgttt cacccctgcc gattcgagtg ttttggcgat ataagctgca accgtttgtt
    28921 tcatggttct ccatctcctg aatgtgataa cggtaacaag tttagttcat ctgacggagg
    28981 gggaagggat gggagagaaa ggaggcacta acggttaaat agcccgatga aaggaatatc
    29041 atcgggcata aggcgattat gcgagaacca aatccccctg cggatggcag gagcaggcca
    29101 gtacgtaacc ttcagcgatt tcggcgtcgg tcagcgtcat tgtactgctc accgtatatt
    29161 caccggaaac cacttttgtc ttacagcagc cgcaaacacc cgcacggcag gcagcgacaa
    29221 ccggaacgtt attgctttcc agcgcctcca gtagcgtggt gccaaccggg gcgtaaaatt
    29281 ctcgtgccgg ttgcagtttg gtgaatttca gaccgctggt cgccgctttt gctactgggg
    29341 tgaagaattt ctctttaaag aaacgcgtca cgccgagcgc tttcacttcc tgctctaccc
    29401 aatccatata cggagccggg ccgcaggtca tcacggtacg tgaagctaag tcaggtacac
    29461 ctgccagcag ttctcgagtg agacgaccag cgataaagcc ttcggtaacg ttattttctg
    29521 ccaccagcgt taccggatag ttacgccact catcggcgaa aataacatcc tgcggcgtat
    29581 gcacgttgta gatcacccgc acatcggcct gtggacggtt cttcgcaagc cagcgacgca
    29641 tcgacataat cggcgtgacg ccgcagcctg ccgccagcaa caggaattta tcttctgctt
    29701 tatcgtcgca ggtaaattcc cccatcgcgt ccgaaagcca gagataatca ccgcgtttta
    29761 catcgcgcgt cagccactgg gagccgacac cgtcatcaat ccgccgcacg gtcagggtga
    29821 tatattcact cacgcctggc gtggaggaaa tggtgtaagc acgcagcgtt tccgctgagt
    29881 ttcgcacgct gaccagtgca tattgcccgg cgcgatatgg gtagtaatcg tggcaaatca
    29941 gggaaatcgt ccacacatcc ggcgtttctt gcgtaatgtg atgaacctgc atccgccacg
    30001 ggcattgatt cgttggcatc gtcatcgaca aactccttac gcgctcaaca gttgcttcat
    30061 gtcttcttca acagtggtga tagaacgcag gccgaatttc tcgttcagca ccgccagcag
    30121 gtctggtgtc aggaaaccag gtgcagtcgg gccggtgacg atatttttca cgcccagaga
    30181 aagcagcgtc agcagaatga cgatcgcttt ctgttcaaac caggagagca ccagcgacag
    30241 cggcagatcg ttcacaccac agcccagttt atctgccaga gtgacagcca gaataatcgc
    30301 tgagtaagca tcgttacatt gacctgcatc taccagacgc ggcagacctt cgatatcgcc
    30361 aaactcaagt ttgttaaagc gatatttacc acaggcgagg gtcaggatca ggcagtcatc
    30421 cggcacgatg gtggcgaaat cggtgaagta gtggcgctcg ccgcgtgcgc cgtcacagcc
    30481 accaagcagg aagatatgac gcagtttttc acggctcacc agatcaatca gcgtatcagc
    30541 agcgccaagc agcgtctggc gaccaaaacc cacggtgata aggtgcggaa tttcgctgta
    30601 cgggaagcct gccatctgtt gcgcctgggt gataaccgca gagaaatcat caccgtccag
    30661 atgacgcacg ccaggccagc caacaatgct gcgggtccag atacgatcgt cataagcgct
    30721 tacggttggg tcgatgatgc agttcgaggt catcacgatg gggccaggga aacgagcgaa
    30781 ctccacttgc tgattctgcc agccgctgcc gtagttaccg accagatgct tgaatttacg
    30841 cagctccggg tagccatgcg caggcagcat ttcgccgtgg gtgtagacat taacgcccgt
    30901 gccttcggtc tgttccagca ggttgtagag atctttgaga tcgtgaccgg aaatcagaat
    30961 gcatttaccc gccgtcgctt tgacgttgac ctgggttggc gtcgggtgac cgtatttacc
    31021 ggtttcgcct gcatccagaa tgctcatcac tttgaagttc atctggccga tttccattga
    31081 acactcaaga agcgcgttca tatcggcagg ccaggtcccc agccacgcca tgattttatg
    31141 gtactgggca taaatatcgt tgtcgtattg accgagaaca tgcgcgtgtt ccatataggc
    31201 cgccgcacct ttcaggccat acaggcacag cagacgcagg ccgagaatgt tttcgccaat
    31261 cgccgcttta tctttgttag gggtaaattc tgctgcctga cgttgcagct cgccgagatc
    31321 atcgctcacc agttgcaggt cagccatcgg gttatcgacg cgcgcgttgg catctacagc
    31381 caggcattgt gctttcagcg cctcgcgcaa ggcaatcgct tcacgagcgt agccgacaat
    31441 acgcggagaa tcgaagttaa cgttggtcag ggttgagaaa aaggcacgtg gcgcgaagct
    31501 gtcaacatcg tggttgatga tgccgtattc acgcgctttt accgcccagg cagaaagccc
    31561 ttgcagcgcc gcgatgagta aatcctgaag gtcagaagtt tccgccgttt taccacacat
    31621 cccctgcgcg tatgagcagc cgtttcctgc cggagtacgg atagtttgtt cacattgcac
    31681 acaaaacatg atcacacctt ttaaagttat atttaatata catgtttaag gttaagacgc
    31741 ttaacgcggg gataaaaggg atttttcatg caactttaag ggagattgat ttagcgcaat
    31801 tttggcggca gggatctacc gccagagagg tattacgcag agaaaaaggc gatgaggatc
    31861 ggcactaaca ggctaagaat aaaaccgtga acaattgccg ccgggaccat atccagcccg
    31921 ccagtacgtt gaagaacggg cagggtgaaa tccattgatg tggcaccgca taagcccagt
    31981 gcagtagagc ggctgcggcg aatcagccca gggatcaaca taatagcaat cagttcacgg
    32041 gccagatcat taaaaaacgc cgcgctcccg attaccggac caaaagattc ggtcaataaa
    32101 ataccggaaa gagaatacca gccgaaaccg gaggccattg ccagcgcggt attgatgggg
    32161 agatcaagaa taaaggcgtt aattaaacca ccaattaatg aactgacaac caccaccacg
    32221 gcgacaatca ttccccggcg attaaggaca atctgcttta aggtcatgcc attattgcgc
    32281 aactgaatac caacgaggaa aagtagcaaa attaacgtgt attcactggc ttcggtcgcg
    32341 tgttgtaaga aagccagtcc actcagacca atggcaaaac caatcactac tacgccgcac
    32401 agttttagcg actccagcgc catcgcaata cgcgacggga gtttttcttg ctgatggtgg
    32461 ttgcgccacg gcaggcctcg ctccagccac atcagggcgg caatattaca cagtaaaata
    32521 acggtaatac tgacggcaga ataatgcaga atcgccaaca ggttactggc gaggttatcg
    32581 agaaacgcca gactgatacc cataaaaaag agaataaggt aaaccatcca gcttaatagc
    32641 tgattaataa cttttaacgc agcttgttgg cgaagcggaa tgaggtaacc cacaatcagg
    32701 ggaaccagaa tgattaacag cccagaaaac atgaaaaccc agtccttgca aagatgaagt
    32761 cgaaatgcgc gatgacacac tactgaaagc ggaaggacga gtaaagttgc aattaaaagg
    32821 aaatgttatg cataaggagc agtagagtat tcgttttcat ttaaagatat tcttgcgctt
    32881 taattacaaa ctgcaccgat gttggtggcg tcaaaatcgc cgaggcgttc cctgaaggcc
    32941 ggggcagccc acatggatgt gggctgaggg cgcgttttac agggatgtta cctcgcgccc
    33001 gacccggtag ccgtaaggga taagtcgagg gcaccgcgca gcggcgattt tgttcgccag
    33061 agcccggggg ggcagggggc ggcggcgatt ggccgccccc tgcgcgctcc ttgcgccagt
    33121 ggcaatatgt ggcttagctc atgaaaggag cgcaacaaga tgatgaatca acatataaca
    33181 acatcttaaa aaaagcctga cattacgcca ggccttctgc gttaattaat cacgcttttc
    33241 cagcagggtc cggtaaatca gaccaccgat aatgccgccg acaattggca ccacccagaa
    33301 gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg tgctgcgcgc
    33361 cgggttaaca gaagtgttag tcaccggaat actaattaag tgaatcaggg ttaaggccag
    33421 accaatagcg atcggcgcaa aacctgccgg cgcgaatttg gcggttgcgc cgtggatcac
    33481 caacaggaaa cctgcactca ataccagttc aactaccagc gcggaaagca tggaataacc
    33541 gcctggtgaa tgctcgccat aaccgttaga agcaaaaccg ctggctgccg cgtcaaaacc
    33601 cgttttacca ctggcaatta aatacagcag cgccgctgca acaataccgc cgacaacctg
    33661 ggcaattacg tagccaacga cttcttttgc cggaaaacgt ccgccagccc ataaaccaat
    33721 agtgaccgcc gggttaaaat gaccaccaga aatatgacca acagcaaagg ccatcgtcag
    33781 aacggtcaga ccgaacgcca acgccacgcc ggcaaaacca atgcctaatt ccgggaagcc
    33841 tgcggccagt acagcactac cacagccacc aaaaacaagc cagaaagtac caaaacattc
    33901 agctgctaat tttctgaaca tatccacctc aattaaaaat tgaccctgtg aaaaatatgg
    33961 tcgttttata gggtcgtcgt aaaaaaatga cgacggaaat aatgcgcggc tattttaaaa
    34021 acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag ggcaacagcg
    34081 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
    34141 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
    34201 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
    34261 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
    34321 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
    34381 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
    34441 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
    34501 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
    34561 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
    34621 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
    34681 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
    34741 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
    34801 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
    34861 cggtgccaaa tgtccctaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
    34921 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
    34981 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
    35041 taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac atcatcaacc
    35101 ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
    35161 ctttattgca ggcaaaaggc acattgagac tggataaaga cgcgcgtcca ttgttgctga
    35221 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
    35281 atcttctgcc attgcagcgc attgccacca ccaactcggg tgagttgctt tcgttaacgc
    35341 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
    35401 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
    35461 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
    35521 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
    35581 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
    35641 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
    35701 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
    35761 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
    35821 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
    35881 ccaaacccga tcttgccatt gaagtggcaa tagaggcagg acgtcgtggt gtggactccg
    35941 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
    36001 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
    36061 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
    36121 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
    36181 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
    36241 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
    36301 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
    36361 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
    36421 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
    36481 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc ttaagcgtga
    36541 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
    36601 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
    36661 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
    36721 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
    36781 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
    36841 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
    36901 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
    36961 aatcattggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
    37021 tgtgattact aacgtaaatt ttagggtttt gttgatattt cgttgaagtt aatgacccgg
    37081 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
    37141 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
    37201 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
    37261 ggcgaccgga aagctggacg cgctgcgtaa ggttgatgtg ggcgcgcagg tcagcggtca
    37321 gttgaaaact ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
    37381 tattgatcct gaacaggctg aaaaccagat taaggaggtc gaagcaacgc tgatggagct
    37441 acgtgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
    37501 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
    37561 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcggaaatca agcgcaatca
    37621 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
    37681 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
    37741 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
    37801 agcggatgta atccacctga agccggggca aaaagcttgg tttacggtgc ttggcgatcc
    37861 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
    37921 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
    37981 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
    38041 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
    38101 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
    38161 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
    38221 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
    38281 gttgaggtgc tgaagggcat caccctcgat atttatgccg gtgagatggt agcgattgtt
    38341 ggcgcttcag gttccggtaa atcgaccctg atgaatattc tcggctgtct ggataaggcc
    38401 accagcggca cctatcgcgt cgccggtcag gatgttgcca cactggacgc cgatgcgctg
    38461 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt actttcgcat
    38521 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga gcggaaacag
    38581 cggctgcttc gcgcccagga gttactgcaa cgactggggc tggaagaccg tacagagtat
    38641 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
    38701 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
    38761 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatatggt gattatcgtc
    38821 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
    38881 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
    38941 gtcaacacgg tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
    39001 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
    39061 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
    39121 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
    39181 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat caccatccaa
    39241 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
    39301 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
    39361 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
    39421 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
    39481 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
    39541 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
    39601 gggcgagtta tgggccagtc gtggctgaac tccattactg tcagggtaaa agaaggattt
    39661 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggaaagaag
    39721 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
    39781 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
    39841 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
    39901 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
    39961 ttggtcggtg gcgcgttggg aataacgctg tcactgttaa ttgctttcac cttgcagctt
    40021 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
    40081 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
    40141 ccagtagatg ctctggtacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
    40201 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
    40261 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
    40321 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
    40381 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
    40441 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
    40501 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
    40561 tttagagaat tagggcgagc cgtcaagcgt ttgacagggg ccagggggca ggtatgaatc
    40621 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
    40681 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
    40741 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
    40801 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
    40861 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
    40921 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
    40981 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
    41041 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
    41101 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
    41161 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
    41221 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
    41281 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
    41341 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
    41401 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggtaaccg gtgcaaacgt
    41461 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
    41521 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
    41581 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
    41641 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
    41701 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
    41761 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
    41821 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
    41881 cgatatcggt tctctgttag cgggcactaa atatcgcggc gactttgaaa aacgttttaa
    41941 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
    42001 caccattatc ggtgcgggtg cagcgtctgg tggccaggtc gatgcggcta acctgatcaa
    42061 accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc aggagttcag
    42121 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
    42181 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
    42241 ccacgacgtg cgttataccg caaaagcggt gcgtgcagcg gtagagctgg cggtgaaata
    42301 cattaacgat cgtcatctgc cggataaagc cattgacgtt atcgacgaag cgggcgctcg
    42361 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
    42421 cgtggtggcc cgtattgcgc gcattccaga gaagagtgtt tctcagagtg accgcgatac
    42481 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
    42541 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
    42601 tggttcgttc ctgtttgccg gccctaccgg ggtcgggaaa acagaggtga cggtacagct
    42661 ttcgaaagcg ttgggcattg agctgctgcg ctttgatatg tccgagtata tggaacgcca
    42721 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggaggttt
    42781 gctgactgat gcggtcatca agcatccaca tgcggtgctg ttgctggacg aaatcgagaa
    42841 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
    42901 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
    42961 ggtacgtgaa actgagcgta aatccattgg tcttatccac caggataaca gcaccgatgc
    43021 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
    43081 ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga
    43141 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
    43201 ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat
    43261 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
    43321 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
    43381 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgttaggtag gttggtcaag
    43441 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaggag gttcagaccc
    43501 tttttttaat gatgatggta agttgttgat aattagtgct gcgggaaggt aaggataaaa
    43561 aagggtgctg caggagaatg ggatggtttt gctttattta caacgggcta aacgtgtagt
    43621 atttgagttc actgccgtac aggcagctta gaaattcaca ggtaacatac tccacccgcc
    43681 caccatgttc actgccgtac agacagataa aatgcgaaaa aaaagctcgc actttcgtac
    43741 gagctcttct ttaaatatgg cggtgagggg gggattcgaa cccccgatac gttgccgtat
    43801 acacactttc caggcgtgct ccttcagcca ctcggacacc tcaccaaatt gttttgctac
    43861 caaacctcat gggtggcaac ggggcgctac tatagggagt tggagtaaaa cggtcaagaa
    43921 gaattttaat gataattatt gtttgctcat actgtaaaca agttgtgcag tatatctaca
    43981 tcgagacaag ttacggactt atacttccaa agtacttcat acatatcaca aaataaaaag
    44041 gccggttaaa ccgacctttt actcgttctt tctcttcgcc catcaggcgg taaaacaatc
    44101 agcgactacg gaagacaatg cggcctttgc tcaggtcgta cggggtcagt tcaacagtca
    44161 ctttgtcgcc cgtcaggatg cggatgtagt ttttgcgcat tttaccggag atgtgtgcag
    44221 taaccacgtg accgttttct aactctacgc ggaacatggt attaggcaac gtttcaagaa
    44281 cggtaccttg catttcaata ttgtcttctt tggccatcta atcctctggg gtatcactac
    44341 cgtaatttga accggcaaga taatgccgaa gttctgtaaa taagtaaaga tttgcgcgct
    44401 aaatcgcaac aaacaggttc ggcacattac tccgaaaaca cacggctaag ccgcaccaaa
    44461 agcgcaacgt ataagggagc ggtgagataa acgatgggcg ttacctgacg cgaaaaattc
    44521 cttatcggca gcggggtaat gagcgtaacc aactctgcga ccgcaattat aacactctgg
    44581 ggagaaatgt gccgaaaaca ttcattcttg tggtgaaaac aagcatcgtg gtacccagaa
    44641 attattcggc aatcgtccga ggcgcatttg attgagataa ttaaggtaat cccggcgggg
    44701 aatttcgcag gcaccaagcg atgctgtgtg atcgttaagg acctggcagt cgataagctt
    44761 accgccatga ccgataaatt cctcacagaa taccagaagc gccgttttag acgcattttc
    44821 catccggctg aacatggact cgccacaaaa tagcgttccc tgggccacgc cgtacatacc
    44881 gccgacaagc tcatcttcac gccagacttc aatggagtgg gcatggccga gttcgtgaag
    44941 gcgatggtag gcttcgacca cgccacgcgt gatccaggtt ccttcttcgc gatcgctggc
    45001 acagccttca atgacctgac caaaagcgta attcatcgtg acacgatagg gcgagcgttt
    45061 atgaaatcgc ttcatactac ggctgatatg cagtgattct ggccatagca ccgcgcgggg
    45121 atcgggcgac caccagagga tggggtcgcc tggagaaaac cacggaaaaa taccacgctg
    45181 gtaagccatt aacaggcgcg cagggctaag atcgccccca agtgccagca ggccgttagg
    45241 ctcacgtaat gcgccttccg gggaagggaa ggctattgaa tggcgagaaa gctgaaccag
    45301 gcgcatgacc gcaaaactcc acgcaagtcg gatcgttcaa taatagctta caaaccctgc
    45361 ttgaactggt aataacgccc ctgtctggca agcagctctg cgtgagtacc ttgctcaata
    45421 atttgcccgt tgtccatcac tattatttgt tggaaacgag agagtccgcg aagtcgatgg
    45481 gtgaccatta acaccgtttt ctcacgcatc atttctgcaa gcaattcaag gatctggctt
    45541 tcggttgtgg catctaagcc ttcggtaggt tcatccagca acaccagtgg cgcatcatgt
    45601 aacagcgcac gggcgatagc cagacggcgc agttcaccac cggagagctg gcgtccgcct
    45661 tcacctaacc aactgttgag acctgcatcc tcgagcagct tctccagacc aacgcgacgc
    45721 aagctctccg ccagagcctc atcactactg ccaggcgagg cgagtaacag attatcacgc
    45781 agcgtggcgc taaacagatg cactcgctga ggaacaacgc tgatggtctg tcgtagagcc
    45841 gcttcattca ggctggctat ggggctatcg ttaagcaaaa tctcgccctg ttgcgggtcc
    45901 caagcgcggg tcagcagttg taacagtgtt gatttgccgc atccggttcg cccgagaatc
    45961 gcaatatgtt ccccggcgtt tacctgaaga gaaatccctt taagtgcctg ttgggattgc
    46021 tccggataag tgaactgaac atcccgtaat gtcagcgaaa cgcgatcggc aacacgagtt
    46081 tgggtatcag ggaaggtgac ttccggtttt tgatccgtta aatcagtgat ccgaacggca
    46141 gaggcaatga cttgccccag atgctgaaat gcacccgtta ctggtgccag tgcttcaaac
    46201 gcggctaacg cgcagaagac aaacagggca attaacgcgc cgggttgagc attgccgcca
    46261 acgccgccag acgccatcca cagcatcagg atcaccgcta acgcgccaat gagcagcatt
    46321 atcgcttgcg acaatgcggt cagttcagat tgacggcgtt gcgcttccag ccattgaatt
    46381 tctgtattct ctagttgcgt gcgataacga tcgctggcac caaaaatggt cagctcagct
    46441 tgcccttgca gccaggccgt cagttgttgg cgatactgtc cgcgaagatg agtcagattt
    46501 tgcccggtgc tttttcccgc acgataaaac agcggtggca tcaggaaaag cgtcagtaac
    46561 ataatgccgc ccagcgtaaa ggcgagggtg aaatcaagga aacttaaccc gattgtcacc
    46621 atcataatca ccacaaaagc gcccaccagc ggcgagataa cgcgcaggta aagatgatcg
    46681 agcgtatcaa catccgccac cacgcgattg agcaattcgc cctgacgata gcgcgccagt
    46741 ccggcagggg agaggggcaa caatttgctg aaggtgtaaa tgcgcagatg ctgcaacacg
    46801 cggaaagtcg cgtcgtgact taccagacgt tcaaaataac gcccggcagt acgggtgatt
    46861 gcggcaccac gcacgcccgc agcgggtagc atatagttga agctgtacag tccggcaacc
    46921 cccgcaaccg ctgaggccga gaggaaccag ccggaaagtg tcaacagtcc gatactggcg
    46981 agcagcgtca caattgccag cacaatacca agacttaaca tccatttatg acgtttatac
    47041 agtgctagat agggtagcaa agcgcgcatt taaatctcct cctgacgatg ggccagtaat
    47101 gtggcgaatg ggccaccagc cacacttaat tccgcgtaac gtccttgctc aataatccgg
    47161 ccatcctgca taacccaaat gacatcccag tcagcaagat cttctaactg gtgggtgacc
    47221 attaacgttg tctggcgcag agaggcggca ttcagcgcct ccattacgcg ctgttcactg
    47281 tgagcatcaa ggctggcagc gggttcatcc aacagtaata gcgaacaggg atttagtaac
    47341 gcacgggcca ccgccacgcg ctgcgcctgc cccacggaaa ggcgggcagc ctggtcgcca
    47401 acaggcgtat caacgccttg tggcaggagc ggtagaaact cgctgaccca ggcgttatcc
    47461 agcgctgctt gtaattcatg ttcgctggca tcaggtcgcg ccagtagtac gttatcccgt
    47521 aatgttgctg ccggtaattg tggattttgc ccaacccagg agagatgttt acgccatgat
    47581 tctggtgata aatcgcgtaa ttctatcccg ttgattcgta gcgatccctg atatgagaga
    47641 aaaccagaaa gcgcgttcaa cagtgaactt ttacctgaac cgctgcgacc aaccaacacg
    47701 gcacgttggc ctgctggcaa agtaaagttc agcggtccgg ccagcgtttt accttccggc
    47761 gacgtgataa acagatcctc ggcctcaatg gtcaccggat cggtcgatgc taattccacc
    47821 tcgccacact gcggatgggc gagcggggtt tccataaacg ttttcagact gtcagctgca
    47881 ccaacagcct gggctttagc atgataaaac gtaccgagat cgcgtaatgg ctggaaaaac
    47941 tctggcgcaa ggatcagggc cagaaaaccc gcagccagcg tcacgccagt atcgtagtga
    48001 ccaaaatcca gctcgccaag ataggaaaaa ccaaagtaga ccgccaccag agcaattgac
    48061 agcgaggtaa aaaattcgag aatgccggag gataaaaatg ccagccgtag cacttccatt
    48121 gtccgttggc ggaaatcttc cgaagcagaa cgaatacttt caatttcagc ttcaccacga
    48181 ccaaaaatac gcaatgtttc catgccgcgc aggcgatcga ggaaatgccc acttaagcga
    48241 gcaagagcga gaaagttacg tcggttagca tcggcagccc ccattccaac cagcgccata
    48301 aacaacggaa ttaacggtgc agtgcccagc agaatgagcg ccgcagccca gtttgagggg
    48361 aagattgcca ccacaatcag caacggcacc gacactgcca gcgccatttg cggcaggtag
    48421 cgtgcatagt aatcatgcat atcgtcaatt tgctcgagta ccagcgtcgc ccagctcccc
    48481 gcaggtttac cctgaatcca cgctggccct gcttgttgca gacggtcgag aacctgccgg
    48541 cggatggcaa agcggatatg ctgcccggcg tgataaccca cccgttcgcg taaccagacc
    48601 acccatgcgc gcagtacaaa ggtcagaacc agtaacgtaa agggaagcag cagggcttca
    48661 cggggaatat tctccataat catatgttgc agaattcgcg ccatgaacca ggcctgggca
    48721 atgatcaata tgccgctcac aaagcccagc agacgagaaa tattcagcca acgttgggag
    48781 atgacgcttt gctgttttaa ccagcgggtt aactcttttt gacgagattt attcattgca
    48841 cgcttagcag gtgagttatc agaattattt gcagagcaat gttacaacgg ggaaaaaata
    48901 aaggcgaccc atagtcgcaa ggtgtcgcct tctttacttt tgttactgat ttgtaaaatt
    48961 attttgcgtc agctaaacca tcgaggtagc gttccgcatc aagtgctgcc atgcagcctg
    49021 taccggccga agtaatggcc tgacgataaa tatgatccat cacgtcaccc gcggcaaaga
    49081 cgccaggaat gctggtctgg gtggcattac catgaatacc cgactgtact ttgatgtagc
    49141 cgttttccag ttccagctgc ccttcgaaaa tggcagtatt cgggctgtga ccgatagcaa
    49201 caaacagacc ggcaacgtcg agtgattcga tgttatcgct gttttgcgta tcgcgcagac
    49261 gaacgccagt gacacccatt tgatcgccgg tcacttcttc cagcgtccgg ttggtgtgca
    49321 gaatgatgtt gccgttctcc actttatcca tcagacgctt aatgaggatt ttttccgcgc
    49381 ggaaaccgtc acggcggtga atcagatgta cttccgaagc gatgttagac agatacaacg
    49441 cctcttcaac cgcggtattg ccgccgccga tgaccgcaac tttctggttg cgatagaaga
    49501 aaccgtcgca ggttgcacaa gcagaaaccc cacggccttt aaaggcttct tcagagggca
    49561 ggccgagata gcgtgcagaa gctccggtgg caataatcag cgcgtcgcaa gtgtattcgc
    49621 cgttatcgcc attcagacgg aacggacggt tttgcagatc caccttgttg atatgatcaa
    49681 aaatgatttc agtttcaaat ttggtggcat gttcgtgcat gcgctccatt aataacggac
    49741 cggtcagatc gtttggatcg ccaggccagt tttccacttc tgtggtggtg gtcagttggc
    49801 cgcctttttc catgccggta atcagcacag gttgcaggtt ggcgcgcgcc gcatagacag
    49861 cagcggtgta tcccgccggg cctgaaccca ggataagcag tttactgtgt ttggtcgtgc
    49921 ccatgagatc cccatagttg ttggcagaca atgggcagga ttgtagggaa tttactgacg
    49981 taaaaaaaga gtatgacgat tttgttaaca atttgtgcaa tcggcagcat cgataagcag
    50041 gtcaaattct cccgtcatta tcacctctgc tacttaaatt tcccgcttta taagctgatt
    50101 aaatgatgaa taaacgcccc tgttaatgaa tatctggcat gttgtactaa aaatcgatgt
    50161 tttgctttga caatcccctg gtgttttgcg aaaacattcg aggaagaaaa aaaacagtat
    50221 tcttatatgc gcataaccat gcatgtaaat accatgttta ccgtgctagt gaaatctacg
    50281 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    50341 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    50401 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    50461 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    50521 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    50581 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    50641 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    50701 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    50761 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    50821 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    50881 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    50941 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    51001 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    51061 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    51121 caaagaagtc acattgacaa agttaagtag cggccgtcgc cttctggaag cgttgctgat
    51181 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    51241 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    51301 tgcgtggctg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    51361 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagtgacg aatacattga
    51421 ttattttgcc gtttcgttac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    51481 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    51541 cttactaagc actacgttac aaccactgct acacagtagc gggggaacta ttgcgctgct
    51601 ctgcgtttgg gctgcgggcc tgacgctgtt caccggttgg tcatgggtga ccattgctga
    51661 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    51721 tgatacctgg gtcgatgaag atgaatatga agatgacgaa gagtatgaag atgaaaacca
    51781 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    51841 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    51901 cggtaagcgg atggatgatg aagaagagat tatctacact gcacgcggtg tggctgccga
    51961 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    52021 tccattatta aacggtgcgc caattaccga acctgttgct gtagcagctg ctgctaccac
    52081 ggcgacacaa agctgggctg cgccggttga acctgtgacc cagacgccgc ctgttacttc
    52141 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    52201 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagccac aatatgcgca
    52261 gcctgcagtg caatataatg agccgctgca acaacctgta cagccacagc agccgtatta
    52321 tgcgcctgca gctgaacaac ctgtgcaaca accttactac gctcctgcgg cggaacaacc
    52381 tgttcagcaa ccgtattacg caactgcgcc ggaacagcct gcgcaacagc cgtattatgc
    52441 ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa gccgaagagc agcaatccac
    52501 ttttgctcca cagtctacat accagactga gcaaacttat cagcagccag ccgctcagga
    52561 gccgttgtac caacagccgc aacccgttga acagcagcct gttgtggagc ctgaacccgt
    52621 tgtagaagag acaaaacccg cgcgtccgcc gctttactat tttgaagaag tggaagagaa
    52681 gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa ccgattccag aaccggttaa
    52741 agaaccagaa ccgatcaaat cttcgttgaa agcgccttct gctgcagcag tacctccagt
    52801 agaagccgct gccgctgttt ctccgctggc atctggcgtg aaaaaagcga cactggcgac
    52861 gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg gcaaatagcg gtggaccgcg
    52921 tccgcaagtc aaagagggaa ttggcccgca gttgccacga ccgaaacgta ttcgcgtgcc
    52981 aactcgtcgt gaactggcgt cttacggtat taagctgccc tcacagcgtg cggcggaaga
    53041 gaaagcccgt gaagcccggc gcaatcagta tgattctggc gatcagtata acgatgatga
    53101 aatcgatgcg atgcagcagg atgaactggc acgtcagttc gcccagacac agcagcaacg
    53161 ctatggcgaa cagtatcaac atgatgtgcc cgtaaacgca gaagatgcag atgctgcggc
    53221 agaggctgaa ctggctcgtc agtttgcgca aactcaacaa caacgttatt ccggcgaaca
    53281 accggctggg gcgaatccgt tctcgctgga tgattttgaa ttttcgccaa tgaaagcgtt
    53341 gctggatgat ggtccacacg aaccgttgtt tacgccaatt gttgaacctg tacagcagcc
    53401 gcaacaactg gttgcaccgc agcagcagta tcagcagcca caacagcagg ttgcgccgca
    53461 gccacaatat cagcagccgc aacaaccggt tgcaccgcag cagcaggata ccctgcttca
    53521 tccgctgttg atgcgtaatg gcgacagccg tccgttgcat aaaccgacga cgccgctgcc
    53581 ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata cctttgcgct
    53641 tgaacaaatg gctcgcctgg tggaagcgcg tctggctgat ttccgtatta aagccgatgt
    53701 cgtcaattac tctccggggc cggttatcac tcgctttgaa cttaacctgg ctcctggcgt
    53761 aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc cgttcacttt cgacggtggc
    53821 ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt taccaaataa
    53881 aaaacgacaa accgtttatc tgcgcgaagt tttggataac gccaaattcc gcgataatcc
    53941 gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg ttgccgatct
    54001 ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaaat ctgtcggtgt
    54061 gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc gtttcatcat
    54121 gatcgacccg aaaatgctgg agctttcggt ttatgaaggc attccgcatc tgttaacgga
    54181 agtcgttact gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta acgagatgga
    54241 gcgtcgttat aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt ataacgaaaa
    54301 aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga agccgggtga
    54361 cagtatggat gcccagcatc cggtgctgaa aaaagaacca tacattgtgg tgttggttga
    54421 cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctga tagcacgtct
    54481 ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg gcaactcagc gtccatcggt
    54541 tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct ttaccgtatc
    54601 cagtaagatt gactcacgta ccattcttga tcaggctggc gcggaatcac tgctggggat
    54661 gggggatatg ctctactctg ggccgaactc cacgttgccg gtacgtgtcc atggtgcttt
    54721 tgttcgcgat caggaagttc atgccgtggt gcaggactgg aaagcgcgtg gtcgcccaca
    54781 gtatgttgat ggcatcactt ccgacagtga aagcgaaggt ggcgcgggcg gtttcgatgg
    54841 cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag tttgtcactg aaaaacgcaa
    54901 agcgtcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg cagcgcgtat
    54961 tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg gtaatcgtga
    55021 agtgctggcc ccaccgccgt ttgactaatt aatgcattgc cggataaggc gcggtagcgt
    55081 cgcatccggc actctatcaa ctgaaaattc agtattttct tttttcctca agctgattat
    55141 tagcctggaa tagagagtag agggaactcc cgatcgggag tgacgtaatt tgaggaataa
    55201 tgatgaaaaa aattgccatc acctgtgcat tactctcaag cttagtagca agcagcgttt
    55261 gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa agtcagcagc ttccacgcca
    55321 gcttcacaca aaaagtgact gacggtagcg gtgcggcggt gcaggaaggt cagggcgatc
    55381 tgtgggtgaa acgtccaaac ttattcaact ggcatatgac acaacctgat gaaagcattc
    55441 tggtttctga cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa gctacggcaa
    55501 cttggctgaa agatgccacc ggtaatacgc cgtttatgct gattgcccgc aaccagtcca
    55561 gcgactggca gcagtacaat atcaaacaga atggcgatga ctttgtcctg acgccgaaag
    55621 ccagcaatgg caatctgaag cagttcacca ttaacgtggg acgtgatggc acaatccatc
    55681 agtttagcgc ggtggagcag gacgatcagc gcagcagtta tcaactgaaa tcccagcaaa
    55741 atggggctgt ggatgcagcg aaatttacct tcaccccgcc gcaaggcgtc acggtagatg
    55801 atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc ggataatact
    55861 tttcaacctc tggccgcgcg tatgcggcca gaaaatttag cacagtatat cggccagcaa
    55921 catttgctgg ctgcggggaa gccgttgccg cgcgctatcg aagccgggca tctgcattct
    55981 atgatcctct gggggccacc gggtaccggc aaaacaactc tcgctgaagt gattgcccgc
    56041 tatgcgaacg ctgatgtgga acgtatttct gccgtcacct ctggcgtgaa agagattcgc
    56101 gaggcgatcg agcgcgcccg gcaaaaccgc aatgcaggtc gccgcactat tctttttgtt
    56161 gacgaagttc accgtttcaa caaaagccag caggatgcat ttctgccaca tattgaagac
    56221 ggcaccatca cttttattgg cgcaaccact gaaaacccgt cgtttgagct taattcggca
    56281 ctgctttccc gtgcccgtgt ctatctgttg aaatccctga gtacagagga tattgagcaa
    56341 gtactaactc aggcgatgga agacaaaacc cgtggctatg gtggtcagga tattgttctg
    56401 ccagatgaaa cacgacgcgc cattgctgaa ctggtgaatg gcgacgcgcg ccgggcgtta
    56461 aatacgctgg aaatgatggc ggatatggcc gaagtcgatg atagcggtaa gcgggtcctg
    56521 aagcctgaat tactgaccga aatcgcaggt gaacgtagtg cccgctttga taacaaaggt
    56581 gatcgctttt acgatctcat ttccgcactg cataaatcgg tacgtggtag cgcacccgat
    56641 gcggcgctgt actggtatgc gcgaattatt actgccggtg gcgatccgtt atatgttgcg
    56701 cgtcgctgtc tggcgattgc gtctgaagat gtgggtaatg ctgatccacg agcgatgcag
    56761 gtggcaattg cagcctggga ttgctttact cgcgttggcc cggcggaagg tgaacgcgcc
    56821 attgctcagg cgattgttta cctggcctgc gcgccaaaaa gcaacgctgt ctacactgcg
    56881 tttaaagctg cgctggcgga tgctcgcgaa cgtccggatt atgacgtgcc ggttcatttg
    56941 cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg ggcaggaata tcgttacgct
    57001 catgatgaag caaacgctta tgctgccggt gaggtttact tcccgcggga aatagcacaa
    57061 acacgctatt atttcccgac aaacaggggc cttgaaggca agattggcga aaagctcgcc
    57121 tggctggctg aacaggatca aaatagcccc ataaaacgct accgttaatg ttatcgttgc
    57181 ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg ccacatctcc catttaattc
    57241 gataagcaca ggataagcat gctcgatccc aatctgctgc gtaatgagcc agacgcagtc
    57301 gctgaaaaac tggcacgccg gggctttaag ctggatgtag ataagctggg cgctctcgaa
    57361 gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg taactcccga
    57421 tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata tcgagccttt acgtctggaa
    57481 gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg agctggatgc tttacaggct
    57541 gaaattcgcg atatcgcgct gaccatccct aacctgcctg cagatgaagt gccggtaggt
    57601 aaagacgaaa atgacaacgt tgaagtcagc cgctggggta ccccgcgtga gtttgacttt
    57661 gaagttcgtg accacgtgac gctgggtgaa atgcactctg gcctcgactt tgcagctgca
    57721 gttaagctga ctggttcccg ctttgtggta atgaaagggc agattgctcg catgcaccgc
    57781 gcactgtcgc agtttatgct ggatctgcat accgaacagc atggctacag tgagaactat
    57841 gttccgtacc tggttaacca ggacacgctg tacggtacgg gtcagctgcc gaaatttgct
    57901 ggcgatctgt tccatactcg tccgctggaa gaagaagcag acaccagtaa ctatgcgctg
    57961 atcccaacgg cagaagttcc gctgactaac ctggtgcgcg gtgaaatcat cgatgaagat
    58021 gatctgccaa ttaagatgac cgcccacacc ccatgcttcc gttctgaagc cggttcatat
    58081 ggtcgtgaca cccgtggtct gatccgtatg caccagttcg acaaagttga aatggtgcag
    58141 atcgtgcgcc cggaagactc aatggcggcg ctggaagaga tgactggtca tgcagaaaaa
    58201 gtcctgcagc tgctgggcct gccgtaccgt aaaatcatcc tttgtaccgg cgacatgggc
    58261 tttggcgctt gcaaaaccta tgacctggaa gtatggatcc cggcacagaa cacctaccgt
    58321 gagatctctt cctgctccaa cgtttgggat ttccaggcac gtcgtatgca ggcacgttgc
    58381 cgcagcaagt cggacaagaa aacccgtctg gttcataccc tgaacggttc tggtctggct
    58441 gttggtcgta cgctggttgc agtaatggaa aactatcagc aggctgatgg tcgtattgaa
    58501 gtaccagaag ttctgcgtcc gtatatgaac ggactggaat atattggcta atacccaatt
    58561 tttctgaatc taaaaagcgc ctgcgggcgc tttttttgtc tccctttgat accgaacaat
    58621 aattactcct cacttacacg taatactact ttcgagtgaa aatctaccta tctctttgat
    58681 tttcaaatta ttctatgtat acaagcctat atagcgagct gctatagaaa taattacaca
    58741 atacggtttg ttactggaat caatcgtgag caagcttgag tgagccatta tgaaaacgaa
    58801 aatccctgat gcggtattgg ctgctgaggt gagtcgccgt ggtttggtaa aaacgacagc
    58861 gatcggcggc ctggcaatgg ccagcagcgc attaacatta ccttttagtc ggattgcgca
    58921 cgctgtcgat agcgccattc caacaaaatc agacgaaaag gttatctgga gcgcctgtac
    58981 agttaactgt ggtagtcgct gcccgctacg tatgcacgtc gtggacggtg aaatcaaata
    59041 tgtcgaaacg gacaataccg gcgatgacaa ttacgacggc ctgcactagg ttcgcgcctg
    59101 cctgcgtggg cgttccatgc gtcgccgtgt ctacaatccg gatcgcctga aatatccgat
    59161 gaaacgagtc ggggcgcgcg gtgaaggcaa attcgagcgc attagctggg aagaagccta
    59221 cgacatcatc gcgaccaata tgcagcgcct gatcaaagag tacggcaacg agtctatcta
    59281 tctgaactat ggcaccggta cgctgggcgg caccatgacc cgctcctggc cgccgggaaa
    59341 taccctggtc gcgcggctga tgaactgctg cggcggctat ctgaaccatt acggcgacta
    59401 ctcctccgcg caaattgcgg aaggtttgaa ctatacctac ggcggctggg cagatggcaa
    59461 cagcccgtcg gatatcgaaa acagtaagct ggtagtgctg tttggtaata accctggcga
    59521 aacgcgaatg agtggcagtg gggtgactta ctatcttgaa caggcacgcc agaaatctaa
    59581 tgcccgcatg atcatcatcg atccgcgcta taccgacacc ggtgccgggc gcgaagatga
    59641 gtggatccct attcgtccgg gaacagatgc cgcactggtt aacggtctgg cgtacgtcat
    59701 gatcactgaa aacctggtgg atcaggcatt cctcgataaa tattgcgttg gctacgatga
    59761 gaaaaccctg ccagccagtg cgccgaaaaa tggccactat aaagcttata ttctgggtga
    59821 agggccagat ggcgtggcta aaacaccgga atgggcctca caaatcaccg gtgttccggc
    59881 agacaaaatc atcaaactgg ctcgtgaaat cggcagtacc aaaccggcgt ttatcagcca
    59941 gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca acccgtgcta tctcgatgct
    60001 ggcgattctg accggtaacg ttggtattaa cggaggcaac agcggcgcgc gtgaaggttc
    60061 atacagctta ccgtttgtcc gtatgccgac cttggaaaac ccgatccaga ccagcatttc
    60121 gatgtttatg tggaccgatg ccattgaacg tggcccggaa atgacggcgc tgcgtgatgg
    60181 tgtacgcggg aaagataagc tggatgtgcc gatcaaaatg atctggaact atgccggtaa
    60241 ctgcctgatt aaccagcatt ctgaaatcaa ccgtacccat gaaatccttc aggatgataa
    60301 gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc tcatcggcga aatatgctga
    60361 catcctgctg cctgactgca ccgcttccga acagatggac tttgcgctgg atgcatcctg
    60421 cgggaatatg tcttacgtga ttttcaacga tcaggtgatt aaaccgcgct ttgaatgtaa
    60481 gaccatctat gaaatgacca gcgaactggc aaaacgtctt ggcgttgagc aacagtttac
    60541 tgaaggccgt acacaggaag agtggatgcg gcatctgtat gcccagtcgc gggaagcgat
    60601 tcctgaacta ccaacgtttg aagagttccg caagcagggg atctttaaaa agcgcgaccc
    60661 acaagggcac cacgttgctt ataaagcttt ccgtgaagat cctcaggcaa attcactgac
    60721 cacgccatcg ggcaaaattg agatttattc gcaggcgctg gctgacattg ccgctacctg
    60781 ggaattgcca gaaggagatg tgatcgatcc actgccgatc tacacgccgg gctttgaaag
    60841 ttatcaggat ccgttgaaca aacagtatcc gctgcagctt acgggtttcc actataaatc
    60901 tcgcgttcac tcaacttacg gcgacgttga tgtgctgaaa gcagcttgcc gtcaggaaat
    60961 gtggatcaac ccgcttgatg cgcaaaaacg cggtatcaac aacggcgata aagtcaggat
    61021 ctttaacgat cttggtgagg ttcatattga ggcgaaagtg acgccacgaa tgatgccggg
    61081 tgtggtcgca ctgggtgaag gtgcctggta tgacccggat acaaaacgtg tcgataaggg
    61141 cggttgtatc aacgtactga ccactcaacg tccgtctcct ctcgctaagg ggaatccgtc
    61201 acatacaaac cttgttcagg ttgaaaaggt gtaaggagta accgatgaca acccagtatg
    61261 gattttttat tgattccagc cgttgcaccg gttgcaaaac ctgcgagctg gcctgtaaag
    61321 actacaaaga tttgacgcca gaagtcagct tccgccgcat ttatgaatat gctggcggcg
    61381 actggcagga agataacggt gtctggcacc agaacgtgtt tgcctactat ctgtcgattt
    61441 catgtaacca ctgcgaagat ccggcttgta ctaaagtctg cccgagcggt gcgatgcata
    61501 aacgtgaaga tggttttgtt gtggtcgatg aagatgtgtg cattggctgc cgctactgcc
    61561 atatggcttg
//
LOCUS       DAJVOY010000006        47218 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVOY010000006 DAJVOY010000000
VERSION     DAJVOY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.006"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            27..764
                     /locus_tag="OQL90_000352"
     CDS             27..764
                     /locus_tag="OQL90_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1367248.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            875..1885
                     /gene="pyrD"
                     /locus_tag="OQL90_000353"
     CDS             875..1885
                     /gene="pyrD"
                     /locus_tag="OQL90_000353"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1367249.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2059..2601
                     /gene="zapC"
                     /locus_tag="OQL90_000354"
     CDS             2059..2601
                     /gene="zapC"
                     /locus_tag="OQL90_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1367250.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="OQL90_000355"
     CDS             complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="OQL90_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1367251.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3951..6059
                     /gene="rlmKL"
                     /locus_tag="OQL90_000356"
     CDS             3951..6059
                     /gene="rlmKL"
                     /locus_tag="OQL90_000356"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1367252.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6071..7978
                     /locus_tag="OQL90_000357"
     CDS             6071..7978
                     /locus_tag="OQL90_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1367253.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8108..9361
                     /gene="pqiA"
                     /locus_tag="OQL90_000358"
     CDS             8108..9361
                     /gene="pqiA"
                     /locus_tag="OQL90_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1367254.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9366..11006
                     /gene="pqiB"
                     /locus_tag="OQL90_000359"
     CDS             9366..11006
                     /gene="pqiB"
                     /locus_tag="OQL90_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1367255.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            11003..11566
                     /gene="pqiC"
                     /locus_tag="OQL90_000360"
     CDS             11003..11566
                     /gene="pqiC"
                     /locus_tag="OQL90_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1367256.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11822..11989
                     /gene="rmf"
                     /locus_tag="OQL90_000361"
     CDS             11822..11989
                     /gene="rmf"
                     /locus_tag="OQL90_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1367257.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="OQL90_000362"
     CDS             complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="OQL90_000362"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1367258.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12646..14406)
                     /locus_tag="OQL90_000363"
     CDS             complement(12646..14406)
                     /locus_tag="OQL90_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1367259.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPNLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14592..15044
                     /gene="matP"
                     /locus_tag="OQL90_000364"
     CDS             14592..15044
                     /gene="matP"
                     /locus_tag="OQL90_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1367260.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="OQL90_000365"
     CDS             complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="OQL90_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1367261.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="OQL90_000366"
     CDS             complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="OQL90_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1367262.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17245..17874
                     /gene="sxy"
                     /locus_tag="OQL90_000367"
     CDS             17245..17874
                     /gene="sxy"
                     /locus_tag="OQL90_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS1367263.1"
                     /translation="MKSLSYKRIYKSQEYLAMLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="OQL90_000368"
     CDS             complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="OQL90_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1367264.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(20009..20455)
                     /locus_tag="OQL90_000369"
     CDS             complement(20009..20455)
                     /locus_tag="OQL90_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1367265.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20578..22638
                     /gene="helD"
                     /locus_tag="OQL90_000370"
     CDS             20578..22638
                     /gene="helD"
                     /locus_tag="OQL90_000370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1367266.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPLPVNRLEEFDNCREAWRK
                     CQAWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLA
                     GAGSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITART
                     FHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEE
                     MQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIK
                     LMAPLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAA
                     LLAALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNS
                     RIGKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERIL
                     ILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAAR
                     ESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPV
                     ARKP"
     gene            complement(22670..23128)
                     /gene="mgsA"
                     /locus_tag="OQL90_000371"
     CDS             complement(22670..23128)
                     /gene="mgsA"
                     /locus_tag="OQL90_000371"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1367267.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVAANVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23224..23886)
                     /locus_tag="OQL90_000372"
     CDS             complement(23224..23886)
                     /locus_tag="OQL90_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1367268.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24059..24472
                     /locus_tag="OQL90_000373"
     CDS             24059..24472
                     /locus_tag="OQL90_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1367269.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24517..24834)
                     /gene="hspQ"
                     /locus_tag="OQL90_000374"
     CDS             complement(24517..24834)
                     /gene="hspQ"
                     /locus_tag="OQL90_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1367270.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24892..26082)
                     /gene="rlmI"
                     /locus_tag="OQL90_000375"
     CDS             complement(24892..26082)
                     /gene="rlmI"
                     /locus_tag="OQL90_000375"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCS1367271.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26177..26455
                     /gene="yccX"
                     /locus_tag="OQL90_000376"
     CDS             26177..26455
                     /gene="yccX"
                     /locus_tag="OQL90_000376"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1367272.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26452..26781)
                     /gene="tusE"
                     /locus_tag="OQL90_000377"
     CDS             complement(26452..26781)
                     /gene="tusE"
                     /locus_tag="OQL90_000377"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1367273.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26872..27531)
                     /gene="yccA"
                     /locus_tag="OQL90_000378"
     CDS             complement(26872..27531)
                     /gene="yccA"
                     /locus_tag="OQL90_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1367274.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27738..27825)
                     /locus_tag="OQL90_000379"
     tRNA            complement(27738..27825)
                     /locus_tag="OQL90_000379"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27789..27791),aa:Ser,seq:tga)
     gene            28252..29370
                     /gene="hyaA"
                     /locus_tag="OQL90_000380"
     CDS             28252..29370
                     /gene="hyaA"
                     /locus_tag="OQL90_000380"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1367275.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29367..31160
                     /gene="hyaB"
                     /locus_tag="OQL90_000381"
     CDS             29367..31160
                     /gene="hyaB"
                     /locus_tag="OQL90_000381"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS1367276.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMINLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            31179..31886
                     /gene="hyaC"
                     /locus_tag="OQL90_000382"
     CDS             31179..31886
                     /gene="hyaC"
                     /locus_tag="OQL90_000382"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCS1367277.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31883..32470
                     /locus_tag="OQL90_000383"
     CDS             31883..32470
                     /locus_tag="OQL90_000383"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1367278.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32467..32865
                     /gene="hyaE"
                     /locus_tag="OQL90_000384"
     CDS             32467..32865
                     /gene="hyaE"
                     /locus_tag="OQL90_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1367279.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32862..33719
                     /locus_tag="OQL90_000385"
     CDS             32862..33719
                     /locus_tag="OQL90_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1367280.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33853..35397
                     /gene="appC"
                     /locus_tag="OQL90_000386"
     CDS             33853..35397
                     /gene="appC"
                     /locus_tag="OQL90_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1367281.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35409..36545
                     /gene="appB"
                     /locus_tag="OQL90_000387"
     CDS             35409..36545
                     /gene="appB"
                     /locus_tag="OQL90_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1367282.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36558..36650
                     /gene="cbdX"
                     /locus_tag="OQL90_000388"
     CDS             36558..36650
                     /gene="cbdX"
                     /locus_tag="OQL90_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1367283.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36730..38028
                     /gene="appA"
                     /locus_tag="OQL90_000389"
     CDS             36730..38028
                     /gene="appA"
                     /locus_tag="OQL90_000389"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1367284.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNV
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38143..40323)
                     /gene="etk"
                     /locus_tag="OQL90_000390"
     CDS             complement(38143..40323)
                     /gene="etk"
                     /locus_tag="OQL90_000390"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS1367285.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLHDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40343..40789)
                     /gene="etp"
                     /locus_tag="OQL90_000391"
                     /gene_synonym="yccY"
     CDS             complement(40343..40789)
                     /gene="etp"
                     /locus_tag="OQL90_000391"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1367286.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40777..41911)
                     /locus_tag="OQL90_000392"
                     /pseudo
     CDS             complement(40777..41911)
                     /locus_tag="OQL90_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
     gene            complement(41957..44053)
                     /locus_tag="OQL90_000393"
     CDS             complement(41957..44053)
                     /locus_tag="OQL90_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1367287.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44053..44799)
                     /locus_tag="OQL90_000394"
     CDS             complement(44053..44799)
                     /locus_tag="OQL90_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1367288.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44796..45440)
                     /gene="gfcB"
                     /locus_tag="OQL90_000395"
     CDS             complement(44796..45440)
                     /gene="gfcB"
                     /locus_tag="OQL90_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCS1367289.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45547..45852)
                     /locus_tag="OQL90_000396"
     CDS             complement(45547..45852)
                     /locus_tag="OQL90_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCS1367290.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46294..46506)
                     /gene="cspH"
                     /locus_tag="OQL90_000397"
     CDS             complement(46294..46506)
                     /gene="cspH"
                     /locus_tag="OQL90_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCS1367291.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46792..47004
                     /gene="cspG"
                     /locus_tag="OQL90_000398"
     CDS             46792..47004
                     /gene="cspG"
                     /locus_tag="OQL90_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCS1367292.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47015..47203
                     /locus_tag="OQL90_000399"
     CDS             47015..47203
                     /locus_tag="OQL90_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1367293.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11628 a        11729 c        12407 g        11454 t
ORIGIN      
        1 tagcgatgtg cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta
       61 atacgtggaa cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg
      121 aattgcgggc aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat
      181 cgatatctat tttactgact aataccagtc aggaatcctg gctgattaac agtaaaatca
      241 accgcccaac gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg
      301 ctccgccact tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg
      361 aaagcgacat cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc
      421 catccggcaa agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt
      481 tctggcgacc cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga
      541 cacggaattc gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc
      601 aggtgagcgt aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc
      661 gtcagacaag ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg
      721 attacgacgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac
      781 gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgc
      841 ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc
      901 ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat
      961 tacaggaacg ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg
     1021 catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga
     1081 gtgcattgac gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc
     1141 acgtccacag ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt
     1201 gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa
     1261 ggcccattat gatggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga
     1321 gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat
     1381 cgccatcaat atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc
     1441 gctggatgat ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca
     1501 taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca
     1561 ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact
     1621 cgatcgttct cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg
     1681 tccgcttcag ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg
     1741 cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc
     1801 tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat
     1861 taaagaaatc gttacccata tctaattatt tcttcgaatt cctctttata agataatcag
     1921 ggctttattt tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg
     1981 tacttttatt gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat
     2041 agggtagggg agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat
     2101 gaagagcacg atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt
     2161 gcgcgcaaaa tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc
     2221 gcgctctatt tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa
     2281 gccgaactgg tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa
     2341 agctggcatt ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc
     2401 gtttggttga gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat
     2461 gccgcactat gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg
     2521 ggcgacgcca ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc
     2581 agcctcgaac aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag
     2641 caaagaatgg tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct
     2701 aaaatctgaa cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt
     2761 ccctgatttt ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc
     2821 tgccagtcaa tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg
     2881 gcagctgcgc cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg
     2941 cgaatcacgc cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc
     3001 tgggcagtgc ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct
     3061 ttttctgggc tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc
     3121 acatcaccaa tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc
     3181 accagattgg ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca
     3241 cgtaacgagg cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca
     3301 gtgttgtggc gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg
     3361 cgggagaaaa atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa
     3421 tgtgtgcccc aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat
     3481 gcgctactgc catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac
     3541 cgtaccatct ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata
     3601 aagatgcgat cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg
     3661 cgcatcgatt taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca
     3721 aatgaaaata agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa
     3781 ttttcttagc gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct
     3841 cgaaatcctc acgtacttgt gtacgctccg ttttctccgc gctgtccatg tccagactga
     3901 ctgcaacaat tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc
     3961 tgtttgccag tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg
     4021 gggccgttga atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg
     4081 tttaccagag cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt
     4141 gtaaggttta cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga
     4201 tgtttaatcc tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca
     4261 acagtcagta cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa
     4321 atctgccgcg tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc
     4381 ataaagaaac cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct
     4441 atcgcgatcg tgctggtatt gcgccgatca aagaaaccct ggcagccgct attgtgatgc
     4501 gatccggctg gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc
     4561 tgattgaagc ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg
     4621 gctttagcgg ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc
     4681 aaactcgcgc ccgtaaaggc ctagctgagt atagctctca tttttacggt tcggacagcg
     4741 acgcacgggt gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga
     4801 tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg
     4861 gcacagtgtt gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg
     4921 cgctgcatag cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt
     4981 tgtttagtgc ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca
     5041 aggcgaaaaa cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc
     5101 cagacagcaa accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca
     5161 aaaaattcga gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg
     5221 atctgccgga atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt
     5281 atgcaccgcc aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg
     5341 ctgcaaccat ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac
     5401 gccagaaggg caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta
     5461 ccgaatataa cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc
     5521 tcgatcaccg tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca
     5581 acctgttctc ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca
     5641 ccaccaccgt ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga
     5701 atggcctaac cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg
     5761 aggcaaatga acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac
     5821 gaatggaaga tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac
     5881 gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg
     5941 atctcgacgg cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct
     6001 cccaggattt cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa
     6061 aggaatagta atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc
     6121 gcttctcgat aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg
     6181 caacggcgcg ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga
     6241 cggtcgcatt atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg
     6301 taacgttgag ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata
     6361 tctgaaacgc tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct
     6421 caacgaactg gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa
     6481 ccgcatcaac gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct
     6541 ttccggcggc tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt
     6601 gctgttgctt gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg
     6661 gttccttaaa accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg
     6721 caatatggcg acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa
     6781 ttacgaccag tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc
     6841 cgagttcgat cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg
     6901 ccgtacccgt aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga
     6961 acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa
     7021 gatcgttttc gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga
     7081 tttttcggcc caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg
     7141 caaaaccacg ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca
     7201 cgttggcacc aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga
     7261 taaaacggtg atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc
     7321 acgccacgta ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc
     7381 ggtacgtgcg ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa
     7441 accaagcaac ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga
     7501 actgctggaa gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg
     7561 tcagtttgtc gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg
     7621 tcgttatgtc ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa
     7681 acagcctgcg gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg
     7741 cagcagtagc aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct
     7801 cgaagatctg gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt
     7861 cagtcagcca catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga
     7921 gctggagcaa gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc
     7981 gcaaaatagt cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg
     8041 tttatcaggc atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg
     8101 agtaccaatg tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat
     8161 gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac
     8221 aacgttaacc gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc
     8281 actgttcatg ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt
     8341 taccagtgaa attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag
     8401 cctcggcacc tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat
     8461 tctgttactg gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt
     8521 gctttttcaa ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag
     8581 tttcgttaaa ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg
     8641 tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga
     8701 cgacatcgcc ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg
     8761 tcaggggctg cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg
     8821 cccgcgttgt agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc
     8881 gctgcttgta acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac
     8941 ggatttatta ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag
     9001 cgaaggctct tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt
     9061 aaagatgatc gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag
     9121 tgaaagaatg catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga
     9181 cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta
     9241 tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat
     9301 gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg
     9361 acagtatgga atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg
     9421 tgtggatatt tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca
     9481 gccatcaggg accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca
     9541 aaaccaccat taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg
     9601 atgatttgac gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc
     9661 ataaagacac cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc
     9721 tgggaacgct gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa
     9781 tggataaata cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc
     9841 gtgtgattct cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc
     9901 gtggctatcg ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca
     9961 gttatcaact gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct
    10021 ggaaagatag tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct
    10081 cattgacaac gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag
    10141 ggcagccagt ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg
    10201 attcgttgta caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc
    10261 tgcaaccggg agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc
    10321 cattctttgc gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga
    10381 ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc
    10441 accttggcga attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca
    10501 ctggtgcact gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc
    10561 gtgaatttaa tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc
    10621 aacgactgat ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac
    10681 aggcaaccag tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg
    10741 atagcatgaa caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt
    10801 caacgttgcg tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca
    10861 acaagatggt ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc
    10921 tgaaaacgct caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc
    10981 cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct
    11041 ggccggatgc agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag
    11101 cggtacacaa agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt
    11161 tcctgactat ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat
    11221 tgccaacaac aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc
    11281 caacctgagt acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca
    11341 ggacacgctc aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt
    11401 cagtggtgag tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga
    11461 aggagtgcaa actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag
    11521 tcaggaagcc gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt
    11581 aagtataacc gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa
    11641 aatttgttcc tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga
    11701 cagtggcgtg aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga
    11761 ttgcacattt agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg
    11821 catgaagaga caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg
    11881 catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg
    11941 gctgggaggc tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt
    12001 taaaaagaaa cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc
    12061 agaaggcaga cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac
    12121 gaccatcaac cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc
    12181 gtttaaagtg aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt
    12241 tcacttcgcc aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga
    12301 accctactag ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa
    12361 tgaagtggca tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc
    12421 ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt
    12481 tcggtgctgg caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa
    12541 ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat
    12601 tgaagcacgc aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa
    12661 ccagttcagc caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg
    12721 cgcgatacgt tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag
    12781 atttaataat aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc
    12841 ttcttctacc gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg
    12901 gataatgaca ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc
    12961 gattttctca tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt
    13021 gatagcgata ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc
    13081 agccatcgag gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc
    13141 tgagaagggg atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat
    13201 catccctttc gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc
    13261 accatcgcca atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg
    13321 acccggaaat tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc
    13381 aatcaggatt tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg
    13441 ccattcacgc tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca
    13501 cagggaggcg acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc
    13561 ttgttcaccg gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg
    13621 ggcaggtaag tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga
    13681 ctccgcatcg acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag
    13741 ctctggctcc atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt
    13801 cagcttcaat ggcatcgaag gcacagagac agggagaggg cgcgactcat caaacgcaac
    13861 ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc
    13921 cagcagtgta cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg
    13981 ctgcagggta atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac
    14041 ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg
    14101 acgtaagcgg atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc
    14161 gctttgtagc gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc
    14221 cttagccagc ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg
    14281 cagccgcggt tgagtatcac tgaataaagg atcgttttcg tcaatcaaat ttggctgagc
    14341 aaatatttcc tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat
    14401 ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact
    14461 acagcacacg tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg
    14521 gagattcttt tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca
    14581 ttgagatcgc tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt
    14641 atctggtgaa gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg
    14701 cccaggaagc cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct
    14761 ggattgacaa gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca
    14821 gacggaagcg ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg
    14881 aatttattgt ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg
    14941 aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt
    15001 cttctctgaa gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt
    15061 caatttacta aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact
    15121 taagcctgcg gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga
    15181 tccggagcca ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca
    15241 gtaaccgggt tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg
    15301 gagatcaggt aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg
    15361 tcagaaccga tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg
    15421 ttgctcagct ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct
    15481 ttgttgaagt tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt
    15541 gccggagctg gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa
    15601 acacccaagc tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg
    15661 ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca
    15721 ccgccagcga agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta
    15781 gtgtctgcac gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt
    15841 gggtaaccca gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg
    15901 ctgcctttgt acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg
    15961 ttaacctggt aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg
    16021 ttgttgatga aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag
    16081 gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc
    16141 gcgatagctg tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat
    16201 ctccaacgag ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac
    16261 cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa
    16321 acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta
    16381 atcttttttg tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct
    16441 ccgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga
    16501 ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc
    16561 tcgtggcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat
    16621 ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga
    16681 tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag
    16741 gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt
    16801 cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt
    16861 gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa
    16921 cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg
    16981 cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc
    17041 ctgtgagtta ctgtatgaat atacagtaca tccagtgaca acaaagatca accctatttt
    17101 cggaaagagc ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc
    17161 ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta
    17221 tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca
    17281 caagaatacc tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc
    17341 ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct
    17401 tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag
    17461 tgtggccgat ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa
    17521 ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg
    17581 cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt
    17641 ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt
    17701 tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa
    17761 ggtgccatta tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca
    17821 gaatgggctg actcacttac gccgaaacag gagtttcctg cggaacttga gtaatctggc
    17881 gttgcagacg accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca
    17941 gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta
    18001 atgcctcatt gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc
    18061 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
    18121 cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt
    18181 cgcgaatttg cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct
    18241 cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc
    18301 cctgatggta ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt
    18361 cgagcatgcg cggcaaattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc
    18421 acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt
    18481 cacccagtaa gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt
    18541 attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc
    18601 cttccagtga tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac
    18661 caataatcct taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca
    18721 aacttgtcag caagatccaa aacccgtgat gcattccggt tatttgaatg atggcgtagc
    18781 cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga
    18841 agtgacggct aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct
    18901 cattttcgtc attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt
    18961 gggcatcaat ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg
    19021 gtgcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa
    19081 aatgcggatc atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg
    19141 cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat
    19201 gttcacgcaa tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg
    19261 cgacaaagta ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc
    19321 gggtcagcag cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag
    19381 ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact
    19441 tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc
    19501 ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca
    19561 gcagatacat cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa
    19621 tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca
    19681 aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca
    19741 gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca
    19801 aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta
    19861 gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg
    19921 caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag
    19981 gacttagcat aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac
    20041 gcgcagcttg cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag
    20101 cggcaatttt aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga
    20161 tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat
    20221 tcaataccgt accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga
    20281 catgaatagc ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc
    20341 gtgtcagcgg taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca
    20401 gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac
    20461 ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc
    20521 acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg
    20581 gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg
    20641 atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat
    20701 caattactgg cgattcactg taagcgtggt ctggtatggg gcgagctgga atttgtactg
    20761 ccggacgaaa aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac
    20821 catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt
    20881 gttttacgcc agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg
    20941 cgtgagcaaa cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg
    21001 ccgttgccgg ttaaccgact ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt
    21061 caggcctggt tgaaagatat tgaaagcgct cggttgcagc ataaccaggc gtataccgaa
    21121 gccatgctta ccgagtatgc ggattttttc cgccaggtcg agtcttcacc gctgaatccg
    21181 gcgcaggccc gggcagtcgt taatggcgag cattctctgt tagtgctggc aggtgcagga
    21241 agcggaaaaa cgtcggtgct ggtggcccgt gcaggctggt tgctggcacg tggtgaagcg
    21301 tcccctgagc aaattttatt gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag
    21361 cggattcgcg aacggctaca taccgaagac attaccgcac gcacgtttca tgcgctggcg
    21421 ctgcatatta ttcagcaggg cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat
    21481 accgctgccc gtcatgaact ctttattgct gagtggcgca agcaatgcag cgaaaagaaa
    21541 gcgcaggcga agggctggcg gcaatggctg acggaagaaa tgcagtggtc agtgccagaa
    21601 ggtaacttct gggatgatga aaaattacag cgtcgccttg cctctcgcct cgatcgctgg
    21661 gtaagtctga tgcggatgca cggtggtgca caggcagaaa tgattgccag tgcacccgaa
    21721 gagattcgcg atctgttcag taaacgtatc aagttgatgg ccccgttatt aaaagcctgg
    21781 aaaggtgcgc tgaaggcaga aaacgctgtc gatttttcgg gccttattca tcaggcgatt
    21841 gtgattctgg agaaaggtcg ctttatcagc ccgtggaagc atattctggt tgatgaattt
    21901 caggatatct cgccgcagcg ggcagcgttg ttagcggcat tacgcaagca aaacagtcag
    21961 acgacgttgt tcgctgttgg tgatgactgg caggcgattt accgattcag cggtgcgcaa
    22021 atgtcgctca ccaccgcttt ccatgaaaac tttggtgaag gcgaacgttg tgatttagac
    22081 acgacttacc gttttaacag tcgtatcggt aaggtggcaa accgttttat tcagcagaac
    22141 ccaggccagc tgaaaaagcc gctaaacagc ttaaccaatg gagacaaaaa agccgtcacg
    22201 ttattggatg agagtcaact tgacgctttg ctggataagc tctctggtta tgccaaaccg
    22261 gaagagcgca ttctgatcct ggcgcgttac catcacatga ggcctgccag cctggaaaaa
    22321 gcggcaacac gctggccgaa gttgcaaatc gactttatga ccattcatgc cagcaaaggg
    22381 caacaggcgg attacgtcat catcgttggc ttgcaggagg gaagtgatgg ttttccggct
    22441 gcggcgcggg agtcgattat ggaagaggcg ctactgccac cggttgagga tttcccggac
    22501 gctgaagaac ggcggttaat gtacgtggcg ctgacccggg cacgccatcg ggtatgggca
    22561 ctgtttaaca aagagaatcc ctctcccttt gttgaaatac tgaaaaatct ggatgtgccg
    22621 gtggcgagaa aaccgtaaga aacaggtggc gtttgccacc tgtgcaatat tacttcagac
    22681 ggtccgcgag ataacgctga taatcgggga tcagaatatc gaccgcgtcg ttgaaatgcg
    22741 gcgactggat tatgaagtct gccgttgcca cgttggcggc gaccggaatg ttccataccg
    22801 tcgccagacg cagcaaggct ttcacgtcag gatcgtgcgg cacggcattt agtggatccc
    22861 agaagaaaat caatacatca attttccctt ctgagatcaa tgcgccaacc tgctggtcac
    22921 cccccattgg gccactcaac atcgcgttga cgttcatgcc ggtcgcgcgg gaaattaagt
    22981 taccggtagt gcctgttgca tacagtacgt gttgttccag taacggttga tgccgttcca
    23041 cccagctcat cagcatttgt ttgcagtgat cgtgtgccac cagcgcaata tgtttccgcg
    23101 caggtaaagt gcgagtcgtc agttccataa tgtacatccg tagtcaactt tcctacagat
    23161 tactgtaagc acttatcgct gcaagataaa gaccgaaaaa gcctgcgcac aggcacaaaa
    23221 atctcaggaa gatggttgtt tttccgccca ctgcaggaaa gtatttcgcg tttgtgggtc
    23281 agccagttta aaccaatact tcagccgttg ttctgtgagc acctgagact gcggtggaat
    23341 agcatccagc tcggaaacac ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa
    23401 ttgcggtagt gaagcgcgtt tcgcggattt gttatagcgt tcaacctcga cctcataatc
    23461 aatcgttttt gccgttgagg taatggcgag aatatccagc tttaccggaa tcggcgtcgc
    23521 atcgccatcc aacaattcaa ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt
    23581 ctccaggcga ggcaggcgaa aattgacctg gttgatgagc tgggtattaa aactgaccac
    23641 tagcggtggg gagatataca gccgttcttc actattggaa agatgaatcg tcttctcaac
    23701 gcgaaacact aactgatgcg gtccattatc cagttcaatg ctgtccgcac cgcgcagcag
    23761 agagctggaa accttcttgc cgtcgagtac cagaagatcg acatcggttg aaagccgcag
    23821 cgtggtggca aaaacggata ccggcaaaca taatgcaatc aaggtggtca cgatgccggt
    23881 tttcataggt agctcctgtc aaaagaccgc ccgaataata atgtgtcaaa atttttcttt
    23941 aatcatactt actaacatat agacatattt cccgcttttg ctctcattca ttcgtattag
    24001 ctgcatggtt ggcatgtcgg ctttgtcgta cactttgtta aacagtctgg aggaaataat
    24061 gaaagaaacc gatattgcag gcattttaac gtccactcac accattgcgc tggtgggggc
    24121 aagcgacaaa cccgatcgtc ccagctatcg cgtgatgaaa tatctactcg accaggggta
    24181 tcatgtcatt ccggtttcgc caaaagttgc cggcaaaacg ctgctgggcc agcaggggta
    24241 tggcacgctg gcagacgttc cggaaaaggt cgatatggtc gatgttttcc gtaattcaga
    24301 agcggcgtgg ggcgtggcac aggaagctat tgccattggc gcgaaaaccc tctggatgca
    24361 actgggggtg attaatgagc aagcggcagt actggcacgg gatgccgggt tgaatgtggt
    24421 gatggatcgc tgcccggcta ttgagatccc tcgcctgggc ctggccaaat aaaaaatccc
    24481 cggaaggcaa aaaccttccg gggattcgtt cagggatcag ttacgcagac gcggggcctg
    24541 gagttgtttg cggatggtct gcgccagctc atccatggat ggctgttcag gatgttcatc
    24601 ctgcaattcg ctacttagct gggcttcagc caggtaggta tgaaccggta ggccgttatc
    24661 gtcctccatc actacgtgat accacggagc ggcgcgaagc tcgtcattta ccgccagctc
    24721 atcaggcgac ggttcagaga gcgaataaac cgggtcgata tccacgacca ctccgagata
    24781 acctaacagg gaatggcgga cctgctggcc gataccgaat ttgctggcaa tcatagtcac
    24841 ctcccgggaa atctttacct ttgatataag ggtaaaattc cacatttcaa gttacatgac
    24901 gcgacaggca aaccctttca gatatagccc ttccggatag gtagcgatca ccggatgatc
    24961 ggctgcctga cggaactgct ctataaattg tacatcacgg ccggcatcaa ttgcggcatc
    25021 cgcgatgatt ttctgaaata aatcgctggt catcagaccg gaacaggaga aagtcaggag
    25081 aataccgcct tcattcagca actgaatcgc cagcatgttg atatctttat agccacgaca
    25141 cgcgcccatc aactggcttt tattctcaac aaacttcggc gggtccatca cgataacgtc
    25201 aaatttttca ccgcgatcgc gataagtacg cagcaattta aagacatcat cacggacaaa
    25261 ctcagccttg ctcagatcca gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc
    25321 ttcctgggag gtatcaacgc tgacaacctg gctgcaaccg cccatcagtg ccgatacggc
    25381 gaaaccaccg gtataggaga aacagttcag cacacgttta ttttcaacgt agcggcgggt
    25441 agccaggcgg ctgtctcgct ggtccaggta gtagcccgtt ttgtgtccgt gctgaatatc
    25501 caccagcagc ttcatgccgt gttcttcaat cggcagcagg gcaggcggca actcgccggt
    25561 gacgaggccc agggtcagct ccatcccttc ttttttacgt accgcaacat cgctgcgatc
    25621 gtaaatcgca cattccgggt acagcgtttg cagggcacta actaatgccg cgcgctgata
    25681 ttctgcccca gcactcagca gttgcagcac cagaaaatta ccgaaacgat cgatagtaat
    25741 acccggcagg ccatcagatt ctccggcgat taaacgatag ctgtcgaggc catctttttg
    25801 cgccagccag tcacgccatt tttgtgcttg ttgcaaacgg cgggaaaaaa aagcaatgtc
    25861 gatagactca gacgggtcaa acgtccagac gcgcgcccgg atttgcgaag ctggcgaata
    25921 agcgccgcgt gctaaccatt ttccctgatg atcaacaata tcgatggttt caccgaggct
    25981 ggctttacct tccatgcggg caacggcccc ggaaaagacc cacggatggc gacgaagtaa
    26041 tgatttttcg cgccctttgg ctaacactaa acgtacactc ataatttact tttctgtcga
    26101 tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga tttgcaacgc gtccattaag
    26161 gataaggaga gcagcaatgt cgaaagtctg cataattgcc tgggtttacg ggcgagttca
    26221 gggcgtagga tttcgctaca ccacacagta cgaagcgaaa agactgggtt taaccgggta
    26281 cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc tgcggtgaag aagggcaggt
    26341 ggaaaaatta atgcagtggt taaagagtgg cggcccgcgt tctgcgcgtg tagaacgggt
    26401 gctcagcgag ccgcatcatc cctcggggga attaaccgat tttcgtatcc gttaaataca
    26461 ttttaccggt tttggcaggc cagcaatttt ggtggcttgc ttagccggac ctttcggaaa
    26521 cagtcggtat aaatagcggc tgttaccttt ttcttcgcca aatttattcg ccatcgcttt
    26581 taccagcata cgaatcgccg gagaagtatt gaattccaga tagaaatcac gcacaaaacg
    26641 caccacttcc cagtgttccg gtgacagcga aatcccttcg ttctctgcaa tcaccactgc
    26701 cagcggctca ctccactggc tgctttcttt gagatagcct tcggtatccg tttctatctc
    26761 tttaccttcg aagatcagca taattattac taccttaatc agactggcgg cagtgtaaca
    26821 aaaaacaaag ccccgcataa agcgaggcta tgaaagtgtt agcgggtgag attaatcgcg
    26881 gctggcgaag cccagaatgc tcagcaggct gacgaagatg ttgtacagcg aaacatacag
    26941 gctaaccgtg gcacgaatat agttcgtttc accgccatga acgatgttgc tggtttcaaa
    27001 caaaatagcg ccagaggaga tcaaaatgaa gaccgcgctg atcgccagat gcagagcagg
    27061 cagctgcagg aagatattag caaccatacc aatcagcacc accacaatac ccgccatcag
    27121 cataccgccg aggaacgaca tatctttgcg ggtggtcagc acatatgcag agcagcagaa
    27181 gaacactaac gccgttccgc ccagagccat agcgattacg tcacccattc cggcagacag
    27241 ataggtgttc agaataggtc cgaggatata acccagaaaa ccggtaaagg cgaatgcgga
    27301 gataatcccg gtcggcttat tcgccgtttt ataggtcagg aacatcaaac catacatacc
    27361 caccagcgtc agaatcagac ccggagatgg cagcatcagc accgtactgg cagttgcggt
    27421 aatcgccgaa aaggccagcg tcaggctcag cagaaaatag gtattacgca gcaccttatg
    27481 ggtgctaagc agtgatgtac ggtcatgtga agaactaaca atacgatcca ttgagtcact
    27541 ctctatgaca gatgtaatta attaagcagc ataatgataa tgcgtaaggg cacccagaag
    27601 ttttacccat ctttacgcat ttgatctgga acaggtttaa cagcggatta tcaggtcatt
    27661 aagcaaatat aacgccctga gaatttcgac aggcaaaaga aaaaggggtt agcatttagc
    27721 taacccctta tcttatttgg cggaagcgca gagattcgaa ctctggaacc ctttcgggtc
    27781 gccggttttc aagaccggtg ccttcaaccg ctcggccaca cttccggaat gacgcgcact
    27841 ataaacatcc cgatgcggcg tgtaaacccc taatttgttt gtttgcctga aaaacagcca
    27901 aaagtgcatt gatagcgtga aataacagca gattgatcat ttcatcacca tgaattcctt
    27961 ctcttttact cgtttagcaa ccggctaaac atccccaccg cccggcaaaa agaaaaatag
    28021 gtccattttt atcgctaaaa gataaatcca cacagtttgt attgttttgt gcaaaagttt
    28081 cactacgctt tattaacaat actttctggc gacgtgcgcc agtgcagaag gatgagcttt
    28141 cgttttcagc atctcacgtg aagcgatggt ttgccttgct acagggacgt cgcttgccga
    28201 ccataagcgc ccggtgtcct gccggtgtcg caaggagaag agacgtgcga tatgaataac
    28261 gaggaaacat tttaccaggc catgcggcgt cagggcgtta cccggcgcag ctttctcaaa
    28321 tattgtagtc tggctgccac gtcgctggga ttaggcgcgg gaatggcacc aaagattgcc
    28381 tgggcgctgg agaacaaacc ccgcattccg gtggtatgga tccacggtct ggaatgcacc
    28441 tgctgtaccg aatcttttat ccgctccgct cacccactgg cgaaggacgt catcctttcc
    28501 ctgatttccc tcgattatga cgatactttg atggctgccg ccggaaccca ggcggaagaa
    28561 gtcttcgaag acatcatcac gcaatacaat ggcaaatata tcctcgcagt agaaggtaat
    28621 ccgccgctgg gcgagcaggg gatgttctgt atcagcagcg gtcgaccgtt tattgagaaa
    28681 ctcaaacgtg ccgctgccgg agccagcgcg attatcgcct ggggaacctg cgcgtcctgg
    28741 ggctgcgtgc aggccgcgcg gcccaatccg acgcaggcaa cgcctatcga caaagtcatc
    28801 accgacaaac ccattatcaa agtacctggc tgcccgccga tcccggatgt gatgagcgcc
    28861 atcattactt acatggtgac ctttgatcgc ttgccagatg tcgacagaat gggccgtccg
    28921 ctgatgttct atggtcagcg aatccacgat aaatgctatc gccgcgccca cttcgacgcc
    28981 ggagagttcg tccagagttg ggatgatgac gctgcccgca aaggttactg cctgtacaaa
    29041 atgggctgca aagggcctac cacctataac gcctgttcct ccacacgctg gaatgatggc
    29101 gtttctttcc caatccagtc tggtcacggc tgcctgggct gtgcggaaaa tggtttctgg
    29161 gatcgcggtt cgttctacag ccgcgtggtc gatattccgc aaatgggtac tcattccacc
    29221 gccgataccg tcggtttaac cgcgcttggc gtggtggcag cggctgttgg tgtgcacgca
    29281 gtcgccagcg ccgttgacca gcgcagacgt cataaccagc aacctacaga aaccgaacat
    29341 cagccaggca atgaggataa acaggcatga gcactcagta cgaaactcag ggatacacca
    29401 tcaataatgc cggacgccgc ctggtggtcg acccgattac gcgcatcgaa ggccacatgc
    29461 gctgcgaagt gaatattaac gatcagaatg tgatcaccaa tgccgtctcc tgcggcacca
    29521 tgtttcgcgg gctggagatc atcctacaag ggcgcgaccc gcgcgatgcg tgggcgttcg
    29581 ttgaacgtat ctgcggcgtc tgtactggcg tacacgccct ggcttcggtt tacgccatcg
    29641 aagatgctat cggtattaaa gtgccggaca acgccaatat catccgcaac attatgctgg
    29701 caacgctctg gtgccacgat catctggtgc acttctatca gcttgccggg atggactgga
    29761 tcgatgtgtt agatgcgctg aaagccgacc cgcggaaaac ctccgaactg gcgcaaagtc
    29821 tctcctcttg gccgaaatca tcccctggct atttcttcga cgtacaaaac cgcctgaaaa
    29881 aatttgttga aggcgggcag ttggggatct tccgcaatgg ctactggggg cacccgcagt
    29941 acaaactgcc gccagaagct aacctgatgg gctttgccca ctatctcgaa gctctcgatt
    30001 tccagcgtga aattgtcaaa atccacgcgg tctttggcgg taaaaacccg catccaaact
    30061 ggattgtcgg cgggatgcct tgcgccatca acattgacga aagcggcgcg gtcggggcag
    30121 tcaatatgga acgcctgaac ctggtgcagt caattatcac ccgcacggcg gacttcatta
    30181 acaacgtgat gatccccgac gccttagcca tcggtcagtt caacaaaccg tggagcgaaa
    30241 tcggcactgg tctttctgat aaatgcgttc tcagctacgg cgcattcccg gatattgcca
    30301 acgactttgg cgagaaaagt ctgctgatgc ctggcggcgc ggtgattaac ggcgacttca
    30361 acaatgtgct gccagtggat ttggttgatc cgcagcaggt gcaggagttt gtcgaccacg
    30421 cctggtatcg atatcccaac gatcaggtcg ggcgtcatcc gttcgatggc atcaccgacc
    30481 cgtggtacaa ccccggcgat gtcaaaggca gcgataccaa cattcagcag ctgaatgaac
    30541 aggaacgcta ctcgtggatc aaagcgccac gctggcgcgg taacgcgatg gaagtggggc
    30601 cgctggcgcg cacgttaatc gcttatcaca aaggcgatgc tgcgaccgtt gagtcggtcg
    30661 atcgcatgat gtcggcgttg aacctgccgc tttccggtat ccagtcaacg ttaggccgca
    30721 ttttgtgccg cgcgcacgaa gcgcagtggg ccgcaggtaa gttgcagtat ttcttcgaca
    30781 agctgatgat caacctgaaa aacggcaatc tcgccactgc ttccacggaa aaatgggaac
    30841 ctgcaacctg gccgacagag tgccgtggtg tcggttttac cgaagcgccg cgcggggcgt
    30901 taggccactg ggccgccatt cgcgatggca agattgatct ctaccagtgc gtggtgccga
    30961 ccacctggaa cgccagcccg cgcgatccca aagggcagat tggcgcttat gaagcggcgc
    31021 tgatgaacac caaaatggcg atccccgagc aaccgctgga gatcctgcgt actctgcaca
    31081 gctttgaccc gtgcctcgcc tgttcaacac acgtgctggg cgacgacggt agcgagctga
    31141 tctccgtgca ggtgcgttaa cagcgaagga gaatcatcat gcaacagaaa agcgacaacg
    31201 ttgtcagcca ctatgtcttt gaagcgccag tccgcatctg gcactggttg acggtgttat
    31261 gcatggcggt gttgatggtc accggatact ttatcggcaa gccgctacct tccgtcagcg
    31321 gcgaggcgac gtatctgttc tatatgggct acatcaggtt aattcacttc agcgccggga
    31381 tggtttttac cgtggttttg ctgatgcgga tctactgggc ttttgttggc aatcgatact
    31441 cccgcgagct gtttatcgtg ccggtatggc gtaaaagctg gtggcagggc gtgtggtatg
    31501 aaatccgctg gtatctgttt ctggcaaaac gtccgagtgc cgatataggc cataatccca
    31561 tcgcccaggc ggcgatgttc ggctatttcc tgatgtcggt ctttatgatc atcactggtt
    31621 ttgcgctgta cagcgaacac agccagtacg ctatttttgc gccgttccgt tatgtggtgg
    31681 aatttttcta ctggacgggt ggcaactcaa tggacattca cagctggcat cggctgggga
    31741 tgtggctgat tggcgcgttt gtgatcggtc atgtctacat ggcgctgcgt gaagacatca
    31801 tgtccgacgg cacggtgatc tccaccatgg tcaacggcta ccgtagccac aaatttggca
    31861 aaataagtaa caaggagtgt tcatgagcga gcaacgcgtg gtggtcatgg ggctgggcaa
    31921 cctgctgtgg gccgatgaag gcttcggcgt gcgggtggcg gaacggctgt atgcccatta
    31981 ccactggccc gagtatgtgg agattgtcga tggcggtact cagggactga acttgctggg
    32041 gtatgtcgaa agcgccagcc atctgttgat tctcgatgcc attgactacg ggctggaacc
    32101 tggaacgctg cgaacctatg ccggagaacg cattccggct tatctcagcg cgaagaaaat
    32161 gagcctgcat cagaacagtt tctccgaagt gctggcgctg gcagatatcc gcggacatct
    32221 gccagcacat attgccctcg tcgggctgca acccgcgatg ctcgacgact acggcggcag
    32281 cctgagcgaa ctggcacggg agcaactgct cgctgcggaa caatcggcgc tggcgcagtt
    32341 ggctgcgtgg ggaagtgtgc cgcaactggc taatgaatcg cgttgtctca attatgactg
    32401 tctgtcgatg gaaaattacg aaggcgttcg cttgcgccag taccggatga cacaggagga
    32461 gcagggatga gcaacgacac gccatttgat gcgttgtggc aacgaatgct ggcgcgcggc
    32521 tggacgccgg tcagtgaatc ccgtcttgac gactggctta cgcaagcgcc agacggcgtg
    32581 gtgttattaa gcagtgaccc gaaacgcacg ccagaggtca gcgataatcc ggtaatgatt
    32641 ggcgaattac tgcacgagtt tcccgactat acatggcagg tggcgattgc tgaccttgag
    32701 cagagcgaag ccatcggcga tcgctttggc gtctttcgct ttcctaccac tttagtgttt
    32761 accggcggaa actatcgcgg cgtgctgaat ggtattcacc cgtgggcgga actgataaac
    32821 ctgatgcgcg ggcttgtcga accgcagcag gagcgtgcct catgagcgaa acttttttcc
    32881 atctgctggg gccaggaacg caaccgaacg atgacagttt cagcatgaat ccactgccga
    32941 tcacctgtca ggtgaatgat gaaccgagta tggcggccct ggagcaatgt gctcacagcc
    33001 cgcaggtgat tgcgctgtta aacgagttac aacatcaact aagcgaacgc cagccgccgt
    33061 tgggcgaggt gctggcagtc gatctgttaa atctcaacgc cgacgatcgt cactttatca
    33121 atacgcttct cggggaaggg gaagtgtcag tgcgcattca gcaggctgac gacagtaaaa
    33181 gtgaaataca ggaggcgatc ttctgcggat tatggcgggt gcgcagacgt cgcggcgaaa
    33241 agttgctgga ggacaaactg gaggctggct gcgcgccgct ggcgttgtgg caggcggcaa
    33301 cgcaaaatct cttgccgaca gattcgctgt taccgccgcc cattgatggc ctgatgaatg
    33361 gcctaccgtt ggcgcatgag ttactggcac atgtacgtaa ccccgacgcg cagccgcaca
    33421 gcattaatct gacgcaatta cccatcagcg aggctgatcg gctttttctc tcacgtctct
    33481 gtgggccggg aaatattcag attcgtacca ttggctatgg cgagagctat atcaacgcca
    33541 cggggttacg ccatgtctgg catttacgct gtacggacac cttaaaaggc ccgttactgg
    33601 aaagttatga aatctgccca ataccggaag tggtgctggc agcgccagaa gatttggtcg
    33661 actctgcgca gcggcttagc gaggtatgtc agtggctggc ggaagctgca ccgacgtaaa
    33721 aagacggtaa gtatcgcttt cagtcttatg aatatcgcaa tcggcgaata cctctggtcg
    33781 tagagtttca ggataaagag ggagatctaa cattatcggg ttatttttct ctcttcgcct
    33841 acaggagtgc gcatgtggga tgtcattgat ttatcgcgct ggcagtttgc tctgaccgcg
    33901 ctgtatcact ttttatttgt accccttacc ctggggctga tttttttgct ggctatcatg
    33961 gaaaccattt acgtggtcac cggcaaaaca atctaccgcg atatgacgcg cttctggggt
    34021 aagctcttcg gtatcaattt tgctcttggc gtggctaccg gcctgaccat ggagtttcag
    34081 tttggtacta actgggcatt ctattccaac tatgtgggcg atatttttgg cgcaccgctg
    34141 gcgatggaag cattaatggc cttcttcctc gaatccacct ttgtcgggct tttcttcttc
    34201 ggctggcagc ggctgaataa ataccagcac ctgctggtga cgtggctggt ggcgttcggt
    34261 tcaaatctct ctgcattgtg gatattgaat gccaacggtt ggatgcaata cccgaccggt
    34321 gcgcattttg atatcgacac cctacgtatg gagatgacca gcttcagcga gctggtcttt
    34381 aacccggtca gccaggtgaa atttgtgcac accgtaatgg cgggctacgt gaccggggcc
    34441 atgtttatta tggcgatcag cgcctggtat ttactgcgcg gacgggagcg cgatgttgca
    34501 ttacgctcgt ttgccatcgg ttccgtcttc ggtactctgg cgattatcgg cacgctacaa
    34561 ctcggggaca gttctgcgta tgaagtcgcg caagtacaac cggtaaaact ggcggcgatg
    34621 gaaggggagt ggcaaacgga acctgctcct gcaccgttcc atgtggttgc ctggccggaa
    34681 caggatcaag agcgtaacgc ctttgccatc aaaattcccg cgctgttagg gatcctcgcc
    34741 actcactcat tagataaacc cgtgccgggt ctgaagaatt tgatggctga aacctaccca
    34801 cgcttgcaac gcggacgtat ggcctggctg ttaatgcagg aaatatcgca aggcaatcgt
    34861 gagccgcatg tgttgcaggc attccgggaa ctggaaggtg acctgggcta cggcatgttg
    34921 ctctcccgct atgcgccgga tatgaatcat gtcacagccg cacagtacca ggcggcgatg
    34981 cgtggcgcga tacctcaggt tgcgccggta ttctggagtt tccgcatcat ggtgggctgt
    35041 ggttccctgc tgctactggt gatgctgatt gcgcttgtcc agacgctgcg tggcaaaatc
    35101 gaccagcatc gctgggtgct gaaaatggcg ctctggagtt tgccgttgcc gtggattgcg
    35161 attgaagccg ggtggtttat gaccgagttt ggtcgtcagc cgtgggcgat acaggacatc
    35221 ttaccgacat actccgcgca ctccgcttta accacaggac aactggcttt ctcactgatc
    35281 atgatcgtag ggctttacac cctgttctta atcgccgaag tctacctgat gcagaaatat
    35341 gcccgtctgg ggccgagcgc gatgcagagt gaacaaccga cgcagcaaca ggggtaaagg
    35401 agaaaatcat gtttgattat gaaacattgc gcttcatctg gtggctgctg attggcgtga
    35461 tcctggtggt ctttatgatc tccgacggat ttgacatggg gatcggctgt ctgctgccgc
    35521 tggtggcgcg taatgatgat gaacgccgga tagtgataaa cagcgttggt gcacactggg
    35581 aaggcaacca ggtctggttg atcctcgctg gtggggcatt atttgccgcc tggcccagag
    35641 tgtatgcagc ggcgttttcc ggcttttatg tggcgatgat cctggtgctg tgctcactgt
    35701 tcttccgccc gctggccttt gattatcgcg gaaaaatcgc cgatgcacgc tggcgtaaaa
    35761 tgtgggacgc cggtctggtc atcggcagtc tggtgccgcc ggtagtcttc ggtatcgcct
    35821 tcggcaactt gttgctcggc gtgccgtttg ccttcacacc gcaattacgc gtggagtatc
    35881 tcggcagctt ctggcaactg ctgacgccat tccctttatt gtgcggattg ctcagccttg
    35941 ggatggtaat tttgcaaggt ggcgtctggt tacaactgaa aactgttggt gtgattcatc
    36001 tgagttcaca gctggccacc aaacgcgctg cactgttggt gatgctgtgc tttttgctgg
    36061 cgggttactg gctgtgggtc ggtattgatg gctttgtact gctcgcccag gatgctaacg
    36121 gtccttccaa tccgttaatg aaactggtgg cagtgctacc tggtgcctgg atgaataatt
    36181 tcgtcgaatc gcccgttttg tggatcttcc cgctgctggg gttcttctgc ccattgctga
    36241 cggtgatggc gatttatcgt ggtcgcccag gttggggatt tttaatggcg tcattgatgc
    36301 aatttggcgt gattttcacg gcaggcatca cgctgttccc cttcgtcatg ccgtcaagcg
    36361 tgagtccgat ctccagcctg acgttgtggg acagcacttc cagccagctg acgctgagca
    36421 ttatgttggt aatcgtgctg atatttttgc ccattgtgtt gctctacact ctctggagct
    36481 actacaaaat gtgggggcgc atgacaacag aaactctccg ccgtaacgaa aacgagttgt
    36541 actaaggagc aggaacaatg tggtatttac tttggttcgt cggcattttg ttgatgtgtt
    36601 cgctctccac ccttgtgttg gtatggctgg acccgcgtct gaaaagttaa cgaacgtagg
    36661 cctgatgcgg cgcattagca tcgcatcagg caatcaataa tgtcagatat gaaaagcgga
    36721 aacatatcga tgaaagcgat cttaatccca tttttatctc ttctgattcc gttaacctcg
    36781 caatctgcat tcgctcagag tgagccggag ctgaagctgg aaagtgtggt gattgtcagt
    36841 cgtcatggtg tgcgtgctcc aaccaaggcc acgcaactga tgcaggatgt caccccagac
    36901 gcatggccaa cctggccggt aaaactgggt tggctgacac cgcgcggtgg tgagctaatc
    36961 gcctatttcg gacattacca acgccagcgt ctggtggccg acggattgct ggcaaaaaag
    37021 ggctgtccgc agcctggtca ggtcgcgatt attgctgatg tcgacgagcg tacccgtaaa
    37081 acaggcgaag ccttcgccgc cgggctggca cctgactgtg caataaccgt acatacccag
    37141 gcagatacgt ccagtcccga tccgttattt aatcctctaa aaactggcgt ttgccaactg
    37201 gataatgtga acgtgactga cgcgatcctc agcagggcag gagggtcaat tgctgacttt
    37261 accgggcatc ggcaaacggc gtttcgcgaa ctggaacggg tgcttaattt tccgcaatca
    37321 aacttgtgcc ttaaacgtga gaaacaggac gaaagctgtt cattaacgca ggcattacca
    37381 tcggaactca aggtgagcgc cgacaatgtc tcattaaccg gtgcggtaag cctcgcatca
    37441 atgctgacgg agatatttct cctgcaacaa gcacagggaa tgccagagcc ggggtgggga
    37501 aggatcaccg attcacacca gtggaacacc ttgctaagtt tgcataacgc gcaattttat
    37561 ttgctacaac gcacgccaga ggttgcccgc agccgcgcca ccccgttatt ggatttgatc
    37621 atggcagcgt tgacgcccca tccaccgcaa aaacaggcgt atggtgtgac attacccact
    37681 tcagtactgt ttattgccgg acacgatact aatctggcaa atctcggcgg cgcactggag
    37741 ctcaactgga cgcttcccgg tcagccggat aacacgccgc caggtggtga actggtgttt
    37801 gaacgctggc gtcggctaag cgataacagc cagtggattc aggtttcgct ggtcttccag
    37861 actttacagc agatgcgtga taaaacgccg ctgtcattaa atacgccgcc cggagaggtg
    37921 aaactgaccc tggcaggatg tgaagagcga aatgcgcagg gcatgtgttc gttggcaggt
    37981 tttacgcaaa tcgtgaatga agcacgcata ccggcgtgca gtttgtaatg cataaaaaag
    38041 agcattcagt tacctgaatg ctctgaggct gatgacaaac gaagaactgt ctaatgcgta
    38101 gaccggaaaa ggcgttcacg ccgcatccgg ccactttcag ttttactctt tctcggagta
    38161 actataaccg taatagttat agccgtaact gtaagcggtg ctggcgcgtt taatcacacc
    38221 attgaggata gcgcctttaa tattgacgcc tgcctgttcc agacgctgca ttgacaaact
    38281 cacctctttg gcggtgttca agccaaaacg cgcaaccagc aggctggtgc caacagaacg
    38341 ccccacgacc gcggcatcac tcaccgccag catcggcggc gtatcgacaa tcaccagatc
    38401 gtaatggtcg ttcgcccatt ccagtaattg acgcatccga tcgcgcatca gcagttcaga
    38461 cgggttaggt ggcacctgac cgcgagtaat cacatcaaag cctcctttgc caaaatgctg
    38521 gatcactttg ttgagctcat ctttacctgc cagatattcc gacaagccat gttcattact
    38581 cacggtaaac aggttatgcg aataaccacg gcgtaagtcg gcatcaataa ataacacttt
    38641 ttgatcggac tgggcgatca ccgctgccag agttgaactg acaaacgttt taccactgtc
    38701 tggcgtcgca ccggtgatca tcagaatgtt attctccgtc tccatcatag cgaagtgcag
    38761 actggttcgt agcgcacgta cggcttccac agcagaatcc gccgggttat ccaccgccag
    38821 gaaggggata tttttagtac gatggcgctg ctgattagaa aataaatttt tcttacgcag
    38881 acgggtacgt ttatccagcc actcggacat tgggatagtg gcataaacgc tgatgccgtg
    38941 ctcttccagt tgttccgggg cttctacacc acgacgcaac atcgcacgcg ccagcacggc
    39001 acccaccgaa ataaacaggc caagaataaa accaagcacc acgttcaacg cttttttcgg
    39061 tttcactggc tgcggctgag tgactgctgg gtcgataatc cgcacgttac caatggcact
    39121 ggatttcgaa atactcaact cctgctggcg gttaagtaat tgcagatata ccgcacggcc
    39181 cgcttctacg tcacggctta aacgtaacac ttcctgttga gtggaaggca ttgccgatac
    39241 ccgcttattc aggcgtttgc gttcttgctc cagcgtctgg cgtttttcca gcagcgcacg
    39301 ataagttggg tgatctttct tatacagctg ggagatctct gcctcgcgga aagtcagctc
    39361 attgagttga ttatcaacgt tcacaatctg ctcaagaacg gctttggctt ctaggttaag
    39421 gtcaaccgaa tcgcgctgct ggcgataaac gttgagtttt tcttccgcct ggtccagctc
    39481 gctgcgcact tcaggcaact ggcgctgtaa gaattcaagg ctttgtgaat cctgcgccgc
    39541 ctggcgagcg atattctgtt gcaaatagtt gttagcgatg ctgttcagaa tacgagtaat
    39601 caactgggga tcatcaccag tcatggtaag ttccagcatc ccgctttctt tactgcgttc
    39661 gctaacggta aaggtttcct gcaatgcgtt aatcgcttcc agttcggtac gctggctcag
    39721 gacaaactgt gttcctggtt tggccttaat gtccgcgata gtcagcgcaa cgccatcttt
    39781 ttccagacgc tggccgacca taccattgac ggtgaactct tcaccttcca gtgtatagtg
    39841 gccgttttcc ccaaccgtga gtgtcagttg ctgatcctga ccattcagtt gtggaatatg
    39901 catccagctg atcgccagct cacctggttt ttctttggtt aatctcgccc agccgcgacc
    39961 cacaatcgga aaatacttct gctcaactat gtcgtgcaga ttcagttcag caatggtttt
    40021 accgagaatc atgcgcgatt gcagcagttg gatctccggt gcagactcgg gcgatgagtt
    40081 agggatcata tcgctcaggc cgctgagaat ggcgttgccc tgtttttgct caacctggac
    40141 cagagtatct gcctgataaa ttggtgtgct taacagcgag taagcgacag cgatcagcgt
    40201 gaataacgcg gttacgctga taataaactt acggtgatcc cataactcgc cgaccagacg
    40261 aagcagatcg atctcatttt cctgagtgct gcctggtggc gtattcatat ttttagttgt
    40321 catcgtattt tataaactca aattaccggc tgaggcgctt cgcccattcc tgactggcgc
    40381 gctccaacat accgtagaca tgttcaaatg cgtcctgact tttacgatag ggatccggga
    40441 tctctttctg ttccagccat tgcccaaaca gcattgtctt cccgcggacc tcgggagcga
    40501 ttgcggtaac ctgagcaata tgctctgact ccattgccag aatcagatcg taatttcgtg
    40561 ccatctcagc agtgagctta cgtccggcat gtccttcaag agatacgcca tgattagcgg
    40621 caacatctgc tgctgtcgca tctgcagggt gttttaccag accatgaaca cctgccgatt
    40681 tgacttttac gcctggcaga cgtttacgca gcaagcgctc gccaatgggc gaacggcaaa
    40741 tgttgccggt acaaaccacc aggattgagt taaattttag ttgggccatg tttgaatcca
    40801 ccgtgaagtc tctgtcaggt catgcacccc ggaaatcgtc ggtaccagca gagaaatcac
    40861 acggttccaa cgagccagcg gcgcggtagt gacataaaca atgtcgtaag gttcaagctg
    40921 gaattccgtt ccgagtatca ttgccgatgc gtccttcgca ttcagctgat aaatattggc
    40981 gattttgcca ttctgcttgt tttgcgttgc acgaataaca aaaatccccg tcgcatcagc
    41041 gacatcctga ttcataccct cagcgttacc cagcgcttcc gccagggtca taccgctgcg
    41101 gtccattttc agcgtgctct gtttacccac ttcacccatc acgaagactt tcagatcatc
    41161 gttgcgcgga ataaataaaa tatcgcccgg gtgcagcagt ttgttctggc gcaagtcgcc
    41221 acgctgcatc agcgcataga ggttcacttt ggtctttacg ccattctggg accacgttgc
    41281 gccagtctgc atctgcggtc agaccgcccg cggcgttaat cgcatccatg atggtcagag
    41341 gaatattggt aatcggctgc tgacccgatt tgctgacttc gccggtgacg tacgcttttt
    41401 gcgatcggaa cgccgcgacg ctgacatcga cttgcgggct ttcaatgact gaatcgagac
    41461 gcgctgtaat ctcgttgcgt acctgggtaa gtgttttccc ggccactttc aggcgaccaa
    41521 tataggggta gaaaatcgca ccatcggcgt taacccagtt gccggtatca ctggcgctac
    41581 ggtattgccc tgcgggggtg gttagttccg ggtgatccca gaccgtaacc atcaacacat
    41641 caccaatccc gatacgatat tcatagtttg cgatttgctg ctccagctcc gggttagcct
    41701 gcgacataat cggtttggcg cgcaactgat caatcaatcc tggcgtgacg ggatagatat
    41761 tgaccatttt gtccaggtca tactgattgt caggtagttc aatgacatcc ttgttcctgg
    41821 tcgataaatt ctgaccagga acaagggtac atgctgtcaa agacagaaca gccaatgtca
    41881 gtaccgaaaa cttaaaaatg ttctttttca ttggtttata tttatcagtc aatgtaatgt
    41941 tatgaattac gtcaggttat tgtccaaccg gtacttctcg ctctgccgtc attggataga
    42001 gctggtattt gcgtgacagc atttgtccac catcacgcgt caatggtgtc cacgaaacca
    42061 ccgcgcggtt gcggttaggt ccaatggtca tcaagtcgaa tggaatcgag atataaaaac
    42121 ctttactaaa gccgccttcg ccgtagtcat ctttagatac gttactgatt gccgcccata
    42181 cccctaccgc cacgccgctg tcaaagcgtt tggcgacgtc gatcgttgct cctttatctt
    42241 ttgccagata ttgcccaacg ctaagtttca tcagtacgcc attgagcgtc ggcgggttcc
    42301 agtaagccgt cacgaaacca gttggcgtgg aataatcggt gaagcgcatc atgttgtccc
    42361 agtcacgttg cttcacgtag ttaacgtcca cacccagcgc ccagctggca tctagcgggc
    42421 gataaagcag ctcggaaccg acaccggcgt acatcgtttc cagataaccg ccatacacct
    42481 ggccatagaa accattgcct aagtcggcaa agtagttcgc ctgcaagttg ttgagataaa
    42541 cgtcattgcg aacgtaatca cggatatgcg tgcgcacgcg gggcagggta gagtccgcgg
    42601 gcaacagcga agacttaaac ttgtcgtagt tgttgtaaat attggtgaaa ataccgccat
    42661 caagcagcag gtggtcggta aaccagtagc gggcactgga catcagcccc agctggaaca
    42721 tatagaaatc ttccgggccg cccagcgact gtgaaagtgt tgggttgaaa gagtagctaa
    42781 agcgatcttc acgaatacgg tagccccgac caaaggcaga aagatcttct gcttcaacac
    42841 gttgttgttg aagttgctct gattgaccag gcgctgtacc agccagctgt ttgcgcaggc
    42901 tggctacgtc ggtttcggta gtcaccatcg ccatatgctc gcgcttttgc gtcacgctaa
    42961 tcttctcaac gccttgcggc aggttattca ccagaatccg gttggcacga tcgaccgctt
    43021 cgcgagaatc acggtatttg tattgttgac cagacatata cagcgtctta tcgcgcagct
    43081 gaatttccgg tgcttcaaaa cctgcgttgt acttcagcgc ggtaagttgg ttcgctactg
    43141 tggtgtactg caatccttca gattcaggcg caggttgata tgccggtttt ggcgtatcgc
    43201 gcagggcagg gcgcagatcg ttgaaattgg tccgtaacgt gaagccaaac atcaacgtgt
    43261 taccgcgttc ataactcagg ttgagatctg cccagctggc agcgcgataa actgcgccga
    43321 cgttgaaatg gcttgcctga ggcagtttgc cagcgaaatc attctggtaa ttgttgccat
    43381 cgtattcgag tttcagacgc aggggattcc acggcgtttg atactcaatg ccgccaaaga
    43441 tggaagccgg gccacgaaag atatcgctaa agctgatatc gcccgcatcg tgagactctg
    43501 cgcgatgaca atatttatcg ttcacccggc aaaacgggtt ggtaatattg cccgcattac
    43561 cggcgtagcc ccatgccatc ccgagggtga aatcaaatgg ccccgcttgc ttgctggcca
    43621 ccagatactc accgtcaaac agacccgtac cagcaatatc acgtttacca aacgccactt
    43681 gcggtagcca gtaaccttct tcccacaggc gaagtttaaa atcgaatgat ttgtctttgt
    43741 atgactgatc gccgctgaaa tcttcccact ggctatattt gcgtgtgcgc acatccgtat
    43801 aacgaatggt gccttccagc catgggaaca gcgccacgga ggtggagtag aaccggtatt
    43861 gatcgttatc ccggtagttg acgctgaatt caccttccgg tgcgatgcgc gcatttggca
    43921 tctgcaacaa tcctgtgccg ccaaagtctg attgcgacga acccagcgga tccgggtagg
    43981 ttaatacttc agcatgacag gcactggaga cggcaatggc caggcagctt aaaagataag
    44041 aattcttctt catcagtcag gaacacgttg cgttaaaaca gacacaatct ggtcatttaa
    44101 atcagcatat ttttcgggca gtacatgcgc agaaaagccc aaccaaagct ggctgccagg
    44161 cggtggttcc acatgtcttt tattccacag ggcaacaggg gcgacgacag tctcaccctc
    44221 aggcgtaatc accataacgt tattcttgtc cgcgcctgca aggcgggggt gatcttgcag
    44281 ataatcggta acgctacggc ctgcctgcca gggcaattgc ccggccccag aaaccgcacc
    44341 cagcagagta atagtcaccg gacgctgtac ggtatagagc gtatagtccc cgaccaacgg
    44401 gggattgctg ttctcatcca ctcgaacaaa atcagggtcc agtttgaccg gtaagcgacc
    44461 agtgatattg agattgagta actgctggcg cacagattta atcgttgccg ccacatcgtc
    44521 gtcagcttct gcttcccagg aagcaagctg tgccatgaca tgttggtagt ctttaagcgc
    44581 tttagctttc gccgcagagt cggtcagcaa ggcaccaggc caccagaggc gatcgcgcag
    44641 ttgcggttgt gtcaccaact ggacaacatt ttccatgggg ccaacggaaa gtgtctgttg
    44701 ttcgccaggc agataaatag tcaccgttcc ttgcgcaaag gcatggggtg tcattacgta
    44761 aagtacgctg gcaatgaaat acgactgtaa tttattcatg gtgcgggttt aagaaacgtc
    44821 atttctacgg gaataacccc tgcgcctaac atttgccgac tttgacgaac ctggccagta
    44881 gccgaatcga cccagaatgt gttttgccac tgtttttctg gccgcgtgga agtcacctct
    44941 tcctgccaga cattacacag cacattgcga cccgcaatat tcagcgtctc taatccagca
    45001 aaggaaaaag tagaactcag ggtcgcagag cgaaaatggt tgtcttcact ccagagaata
    45061 tcgcgcgtcc agcggctgcc atcttttatt gccagcgcgt tacgcagagg gtcctgcccg
    45121 gaatttgtca cttccagcag attattatta agtttgacgg tttttaaaag acgtccattg
    45181 tgggtaacca gcatggcgtt atcctgggat aaccatttgc tttgttcttg ttctatatat
    45241 cccagtacca caaagattcg ttgcccttca ttaaggcgta aatacatcgt ggaataaggc
    45301 aacgcctgga tctgctgatc cgctacggtg atatcctgat tatcgaaaag gctggcgcga
    45361 aatgtatcga ccatactttg ctggctgtgc gtacatcccg caagaaatag tgcgaaaatc
    45421 gataaaataa gagggcgcac ctttaatcct tgaaaggtta attaaaaaaa tgcaaatctt
    45481 taaagcccgg tacgaatatc gcaaccgggt atcatttaac tttaatttcg acgcgttaaa
    45541 gcagtattag cgtgtagtgg acgtggtggt tgaggtgtta tgagaaccgc catcgccact
    45601 gtcatttgct gcaacaactg ccaacgtcgc cacaccaaca gccgttgcca caccgacaac
    45661 tgtcgccgtg ccgacagcgc cagcagccac gccaccagtg gtagtagcag tcgtggttgc
    45721 gccggtagtg gtcgttgtgg tgccagtagt acccgtcgtt gcctcggtcc cgtttgttgc
    45781 ttcaggggca gcaaacgcag ctggcgtcgt caacatgaag gccataagga ttgcagaaag
    45841 cttgtgtttc ataacttttc ctttattcat cgcatggaca atacgtacgc ttgagatcag
    45901 gattgtctac tttgagtata acatccttgt gaaatctccc tttcctcata ccgcttgcac
    45961 tatatactgc gggtaggaaa ggcgcgcaga gggaaatata agattgttaa ctatccccct
    46021 ctggaaatac gacttgtgag cataagataa atcttagcaa aatacaaatt aaagataagg
    46081 aaattgttac gaaagctatt aattattgct ggtaatatag tttcaagtgg tactataact
    46141 ggctgttaaa tggcatttat aactattagg tgcagagata ttcgcttaaa tggattagtt
    46201 tctttgtgga aaagtaactg atgttataat atatgatgtt gactattgca ctattccaat
    46261 aaaaccagtc agctttaaac aagcagcgtc atattaagag agataaacat ttgccgctgt
    46321 tggtcctcgc aggccattta cgcggcaaaa ttccacacgt aatcctggta taagcacttc
    46381 tgcgtcgcgg ggagtgaatg cggaaatatg gacctgaact tctttacgac cgtcggaggg
    46441 gataatgaat cctttgccgc ttttgcgatc aaaggttttg acaattcctg tcattttacg
    46501 ggacaaacaa attccttact gaaaatactg cgctgcacta tacggggtta ataaaataaa
    46561 gccagcgata tttaagaccg ccggacggct aaaataaaat ttgcttaatc tcaattatca
    46621 tgcgttaata gctgcgtcgg tttgaaagac agacagcata caaagtagtt tactaaagca
    46681 gttctcatta tcaggcatta tccccttctt ttgagtctct ctcctgaaca ctaagtagtt
    46741 tctgtattaa agccctgttt gccgaaaggc ccaaaatgaa ggaagtaaaa tatgtctaat
    46801 aaaatgactg gtttagtaaa atggtttaac gcagataaag gttttggctt tatcactcct
    46861 gatgatggca gcaaagacgt tttcgtccat ttcactgcca tccagagcaa tgaattccgc
    46921 acgctgaacg aaaatcagaa agttgaattt tctattgagc aggggcaacg tggccccgcg
    46981 gcagcgaacg ttgttacgct ctaaggttgc cgttattact caacatctcc atttccgctg
    47041 tccatgttgt catggttcac agtaccgcac atcggcattc gatgtgacgg agcgaaatcc
    47101 tttgggcgct aagtgtattt tttgtaaatc gacgatgatc acctttgata acgtcacgct
    47161 gcaaatacgc actgaccatg cgccgctgga tttcacaaaa taatatcagg ctccctcg
//
LOCUS       DAJVOY010000007        44371 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVOY010000007 DAJVOY010000000
VERSION     DAJVOY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.007"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            83..607
                     /gene="lpfE"
                     /locus_tag="OQL90_000400"
     CDS             83..607
                     /gene="lpfE"
                     /locus_tag="OQL90_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCS1367294.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            860..2551
                     /gene="eptB"
                     /locus_tag="OQL90_000401"
     CDS             860..2551
                     /gene="eptB"
                     /locus_tag="OQL90_000401"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1367295.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2643..2719
                     /locus_tag="OQL90_000402"
     tRNA            2643..2719
                     /locus_tag="OQL90_000402"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2677..2679,aa:Pro,seq:cgg)
     gene            3463..5070
                     /gene="dppA"
                     /locus_tag="OQL90_000403"
                     /pseudo
     CDS             3463..5070
                     /gene="dppA"
                     /locus_tag="OQL90_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            5378..6397
                     /gene="dppB"
                     /locus_tag="OQL90_000404"
     CDS             5378..6397
                     /gene="dppB"
                     /locus_tag="OQL90_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1367296.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6407..7309
                     /gene="dppC"
                     /locus_tag="OQL90_000405"
     CDS             6407..7309
                     /gene="dppC"
                     /locus_tag="OQL90_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1367297.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            7320..8303
                     /gene="dppD"
                     /locus_tag="OQL90_000406"
     CDS             7320..8303
                     /gene="dppD"
                     /locus_tag="OQL90_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1367298.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8300..9304
                     /gene="dppF"
                     /locus_tag="OQL90_000407"
     CDS             8300..9304
                     /gene="dppF"
                     /locus_tag="OQL90_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1367299.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9334..10605)
                     /locus_tag="OQL90_000408"
     CDS             complement(9334..10605)
                     /locus_tag="OQL90_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1367300.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11081..11188
                     /locus_tag="OQL90_000409"
     CDS             11081..11188
                     /locus_tag="OQL90_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1367301.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11275..12954)
                     /gene="bcsG"
                     /locus_tag="OQL90_000410"
     CDS             complement(11275..12954)
                     /gene="bcsG"
                     /locus_tag="OQL90_000410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1367302.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12951..13142)
                     /gene="bcsF"
                     /locus_tag="OQL90_000411"
     CDS             complement(12951..13142)
                     /gene="bcsF"
                     /locus_tag="OQL90_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1367303.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13139..14710)
                     /gene="bcsE"
                     /locus_tag="OQL90_000412"
     CDS             complement(13139..14710)
                     /gene="bcsE"
                     /locus_tag="OQL90_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1367304.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14983..15171
                     /locus_tag="OQL90_000413"
     CDS             14983..15171
                     /locus_tag="OQL90_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1367305.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15183..15935
                     /gene="bcsQ"
                     /locus_tag="OQL90_000414"
     CDS             15183..15935
                     /gene="bcsQ"
                     /locus_tag="OQL90_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1367306.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15932..18550
                     /gene="bcsA"
                     /locus_tag="OQL90_000415"
     CDS             15932..18550
                     /gene="bcsA"
                     /locus_tag="OQL90_000415"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCS1367307.1"
                     /translation="MSILTRWLLIPPVNARLIGGYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRP
                     ERSETAQPSDQALAQQ"
     gene            18561..20899
                     /gene="bcsB"
                     /locus_tag="OQL90_000416"
                     /pseudo
     CDS             18561..20899
                     /gene="bcsB"
                     /locus_tag="OQL90_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            20906..22012
                     /gene="bcsZ"
                     /locus_tag="OQL90_000417"
     CDS             20906..22012
                     /gene="bcsZ"
                     /locus_tag="OQL90_000417"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1367308.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21994..25467
                     /gene="bcsC"
                     /locus_tag="OQL90_000418"
                     /pseudo
     CDS             21994..25467
                     /gene="bcsC"
                     /locus_tag="OQL90_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            25549..27537
                     /gene="hmsP"
                     /locus_tag="OQL90_000419"
     CDS             25549..27537
                     /gene="hmsP"
                     /locus_tag="OQL90_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1367309.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27720..29006
                     /gene="dctA"
                     /locus_tag="OQL90_000420"
     CDS             27720..29006
                     /gene="dctA"
                     /locus_tag="OQL90_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1367310.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPVSVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29226..30722
                     /locus_tag="OQL90_000421"
     CDS             29226..30722
                     /locus_tag="OQL90_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1367311.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30818..31747)
                     /gene="kdgK"
                     /locus_tag="OQL90_000422"
     CDS             complement(30818..31747)
                     /gene="kdgK"
                     /locus_tag="OQL90_000422"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1367312.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31979..32746
                     /gene="pdeH"
                     /locus_tag="OQL90_000423"
     CDS             31979..32746
                     /gene="pdeH"
                     /locus_tag="OQL90_000423"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1367313.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32816..34876
                     /locus_tag="OQL90_000424"
     CDS             32816..34876
                     /locus_tag="OQL90_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1367314.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEQLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35110..36432)
                     /locus_tag="OQL90_000425"
     CDS             complement(35110..36432)
                     /locus_tag="OQL90_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1367315.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36843..37856)
                     /gene="yhjD"
                     /locus_tag="OQL90_000426"
     CDS             complement(36843..37856)
                     /gene="yhjD"
                     /locus_tag="OQL90_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1367316.1"
                     /translation="MTQENEIKRPTQDLELEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMLGK
                     TQP"
     gene            complement(37905..38804)
                     /locus_tag="OQL90_000427"
     CDS             complement(37905..38804)
                     /locus_tag="OQL90_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1367317.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            39324..39926
                     /locus_tag="OQL90_000428"
     CDS             39324..39926
                     /locus_tag="OQL90_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1367318.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39977..41626)
                     /locus_tag="OQL90_000429"
     CDS             complement(39977..41626)
                     /locus_tag="OQL90_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1367319.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42030..43427
                     /gene="ccp"
                     /locus_tag="OQL90_000430"
     CDS             42030..43427
                     /gene="ccp"
                     /locus_tag="OQL90_000430"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1367320.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43638..>44371
                     /locus_tag="OQL90_000431"
     CDS             43638..>44371
                     /locus_tag="OQL90_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1367321.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAA"
BASE COUNT        10661 a        11134 c        12062 g        10514 t
ORIGIN      
        1 ccgttagcac taccggaaaa ttacccgctg ccggggatta tagcggtatt gctaccatac
       61 atgtcgaact ggagtaacag tcatgaagcg aacaatactt gcgttatgcc tgctgcctct
      121 ttccttttcg tcattcgcgg caaccacgga tttagggccg aaaggaacat tgaaattcac
      181 ccttaaaatc agccagggtg cttgcgaact ggtgaaagac agcgttgagg ttgacatggg
      241 caccgcggtt ctcaaaaagc cggtaagaat cggaacggaa attaatccca ctcctttttc
      301 catcggcctg aaaaactgta gcgaagtggt cagagcctac gtgactatgg acggtacacc
      361 caatgcagac gactcaaatc tttttgcgct ggatgcgggt ggcgctacag gcgttgggct
      421 gaaaatcaaa accgctgctg gagtacaaca gttcccggaa aatacaacgc cgacaccggt
      481 tgagtttgca attaacggtg atggcgcaca tcaactgaac tatatcgcca gctacgtgcc
      541 tattcgcgcc gatgcaacta ttggtcgcgc agatgcaacg gtcgacttta ctgtacagta
      601 cgaataattt cacatcttaa gggccagtta actggccctt tttactgctt tttgtaacgt
      661 taaagtctct tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc
      721 acactttttc gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact
      781 acctccgcac ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt
      841 ttgtccaggg tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt
      901 tttgcttgca atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg
      961 cagctatgcg cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc
     1021 caccgtactg gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg
     1081 gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt
     1141 ccttaatgtg gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct
     1201 gtcaaaagaa gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc
     1261 tcttatcctt atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc
     1321 agggcagcgt attcgcagcc tggcggtcgt cgtactggcg ggtattatgg tttgggcacc
     1381 gattcgtttg ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc
     1441 gagttatggc ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg
     1501 gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc
     1561 gaagaagttt acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat
     1621 cggtgaaacc acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc
     1681 gaaactggcc caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac
     1741 caaactctca ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg
     1801 cacattaaaa gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct
     1861 ctacgctatg cagagcgaaa tgtggttcta cagcaacacg atggcggaca acattgctta
     1921 tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct
     1981 ggtagacgaa atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct
     2041 gcataccaaa ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg
     2101 gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta
     2161 tgacaactcg gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga
     2221 taagaaagcg attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca
     2281 cctgcacggc acgccgcgtg aattggcacc gccggagcag ttccgcgtac cgatgatggt
     2341 ctggatgtca gataaatatc tggaaaatcc ggccaatgcg caggcatttg cgcagctgaa
     2401 aaaagaagcc gacatgaaag tgccacgccg tcacgtagag ctgtacgata ccatcatggg
     2461 ttgtcttggc tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat
     2521 cccgcaggca aaagaggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa
     2581 aggcattttg ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca
     2641 ttcggtgatt ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg
     2701 aatcctctat caccgaccaa attcgaaaag cctgctcagc gagcaggctt ttttgcattt
     2761 gaagcccatg aggatgagaa cctccggggg ctgggaggtt cgactcgacg taggcctgat
     2821 aagacgcgcc agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac
     2881 cgccttattc gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact
     2941 atccattaac ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt
     3001 tttgcgttac ttatggctgg cgctggcatt ttccgctgtg cgttttagcg ttcatggtat
     3061 taatcgctca gatatccacc ttttcctcag atttttttca ttaaatgcat aaaacttaat
     3121 caccaaatgt tgctaaatat taagctgatt gttctggtta tgttggagta gcacaaattt
     3181 ctctgctgca aatagctgtt tgaagttttt taatctttgt ttgtgggttc tcaccgttgg
     3241 tgtaaatccc ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg
     3301 aggggcattt tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca
     3361 cctcataacg ttgacccgac cgggcaaaaa acaaaaaagg tcaggtagcg acaacccact
     3421 gcaaagggtt aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa
     3481 aagtcaggga tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt
     3541 caggctaaaa ctctggttta ttgctcagaa ggctctccgg aagggtttaa cccgcagctg
     3601 tttacctccg gcaccaccta tgacgcctct tccgtaccgc tttataaccg cctggttgaa
     3661 tttaaaatcg gcaccactga agtgataccg ggcctcgctg aaaagtggga agtcagctaa
     3721 gacggtaaaa tctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa
     3781 ttcaaaccga cgcgtgaact gaacgccgat gacgtggtgt tctcgttcga ccgtcagaaa
     3841 aacgcgcaaa acccgtacca taaagtctct ggcggcagct acgaatactt cgaaggcatg
     3901 ggcttgccgg agctgatcag tgaagtgaaa aaggtggacg acaacacagt tcagttcgta
     3961 ctgactcgcc cggaagcgcc gttcctcgct gatctggcaa tggacttcgc ctctattctg
     4021 tcaaaagaat atgctgatgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac
     4081 ccaatcggaa ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac
     4141 aaagcgtttg atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt
     4201 acccctgatg cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg
     4261 tacccgaacc cggcagatat cgctcgcatg aagcaggata aatccatcaa cctgatggaa
     4321 atgccggggc tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac
     4381 gtgaaagttc gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt
     4441 tatcagggcg cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac
     4501 gacgacgttc aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg
     4561 ggtctggaaa aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac
     4621 ccgaacgctc gctgcatggc ggagatgatt caggcagact gggcgaaagt gggcgtgcag
     4681 gccaaaatcg tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat
     4741 cagacggtaa tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc
     4801 ctgttcagct gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg
     4861 tttgaagatc tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg
     4921 tacaagcaat cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc
     4981 accgtgtttg aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa
     5041 catcacttcg aaaatgtctc tatcgaataa ttaaaagcca tacaagactg atggtaaagg
     5101 caaaactgcc tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt
     5161 tgcacgattt tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac
     5221 tttgtcaaca atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag
     5281 caatacttca ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc
     5341 agttccaggc tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg
     5401 tttgggactc gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca
     5461 catgatcccg ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg
     5521 tcacgcgcag ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca
     5581 ttacatttgg ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt
     5641 atgggaagag ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat
     5701 ttttgctacg gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat
     5761 tttcgatcac actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg
     5821 gggcatgatg ctgatcatgc tggtttcggt gcactggaac ctgacgcccg cctccggtcg
     5881 cgtgagcgat atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga
     5941 caccgccatc tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc
     6001 tgccattgtg ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat
     6061 gctggaagtg ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat
     6121 gcgggtgatt atcgtccatg cgctgcgtaa cgcgatgctg ccggtggtga ccgttatcgg
     6181 cctgcaggtg ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc
     6241 cggtctggga cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg
     6301 cggcgtattg ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta
     6361 cggcgtggtg aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac
     6421 tgaaaataaa gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta
     6481 ttttaaacgc aacaaaggcg cggtcgtcgg gctggtttac gtcgtcatcg tgctgttcat
     6541 cgcgatcttt gccaactgga ttgcacccta taacccggcg gaacagttcc gcgatgcact
     6601 gctcgccccg ccagcctggc aggaaggcgg cagcatggcg cacttgctgg gcaccgatga
     6661 cgtaggccgt gatatgctgt cgcgcctgat gtacggtgcg cgcctgtcgc tgctggttgg
     6721 ctgtctggta gttgtgttat cgctgattat gggcgttatt ctcggcctga tcgccggtta
     6781 ctttggcggc ctagtcgata acatcattat gcgcgtggtc gatatcatgc tggcgctgcc
     6841 aagtctgctg ctggcgctgg tgctggtggc aattttcggc ccgtcgattg gtaacgccgc
     6901 gctggcactg accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct
     6961 ggtggaagtt aaccgcgatt acgtcaccgc gtctcgcgtg gcgggtgccg gggcgatgcg
     7021 tcagatgttt attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct
     7081 cggtttctct aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca
     7141 gccgccaacg ccggagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc
     7201 ctggtgggtc gtgaccttcc cgggtctggc gatcctgctg acggtgctgg catttaacct
     7261 gatgggtgac ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga
     7321 tggcgttatt aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc
     7381 gcgccgtaga ccgcatcagc tacagcgtaa aacagggcga agtggttggg attgtgggtg
     7441 agtccggctc cggtaagtcg gtcagttccc tggcgattat ggggctgatt gattatccgg
     7501 gccgcgtaat ggcggaaaaa ctggtgttta acggtcagga tttgcagcgt atatcagaaa
     7561 aagagcgccg caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca
     7621 gccttaaccc gtgctacacc gtgggtttcc agatcatgga agcgattaag gtgcatcagg
     7681 gcggcaacaa aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc
     7741 ccgatccggc ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc
     7801 gcgtgatgat cgccatggcg attgcctgtc gaccaaaact gctgattgcc gatgagccga
     7861 ccaccgcgct ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc
     7921 agaaagagaa catggcgctg gtgttgatta cccatgacct ggcgctggtg gcggaagctg
     7981 cgcataaaat cattgtgatg tatgcaggcc aggtggtgga aacgggcgat gcgcacgcca
     8041 tcttccatgc gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg
     8101 ctcaggacaa agaacgtctg gcgtcgttgc caggtgtcgt tcccggcaag tacgaccgcc
     8161 cgaacggctg cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag
     8221 aaccggcgct gaatatgctc gctgacgggc gtcagtccaa atgccattac ccacttgatg
     8281 atgccgggag gccgacacta tgagtacgca agaggccacc ctgcaacaac cgctgttgca
     8341 ggctatcgac ctgaaaaaac attatccggt gaagaaaggc atgttcgcgc cggaacgtct
     8401 ggttaaagcg ctggatggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt
     8461 gggcgaatct ggctgcggta aatcgaccct cggtcggttg ctgacgatga ttgaaacgcc
     8521 caccggtggc gagctgtatt accaggggca ggatctgctt aagcacgatc cgcaggcgca
     8581 gaagctgcgt cggcagaaaa tccagatcgt cttccagaac ccttacggtt cgctgaatcc
     8641 gcgtaaaaaa gtcgggcaaa ttcttgaaga gccgctgctg atcaacacca gcttaagcaa
     8701 agaacagcgt cgggaaaaag ccctgtcgat gatggcgaaa gtcggcctga aaaccgagca
     8761 ctatgaccgc tatccgcata tgttctccgg cggtcagcgt cagcgtatcg ccatcgcccg
     8821 tggtctgatg ctcgacccgg atgtggtgat tgccgatgaa ccggtttccg cgctggatgt
     8881 ttcagtgcgc gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctgtc
     8941 ttatgtcttt atctcccacg acctgtcggt ggtggagcac attgctgatg aagtgatggt
     9001 gatgtacctg ggccgctgcg tggagaaggg aacgaaagac caaatcttca ataacccgcg
     9061 ccatccgtac actcaggcgc tactttccgc gacgccgcgc ctgaacccgg acgatcgccg
     9121 cgagcgcatc aagctcagcg gtgaactacc aagcccactg aatccaccgc cgggttgcgc
     9181 cttcaacgcc cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa
     9241 agactacggc ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg
     9301 ttaactcaca aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga
     9361 caagcatagc aaaatgccgt aaagcagaat tagccaggct ttaaatccgc gaagtttttc
     9421 cagttgtgcg actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa
     9481 cgggcttccc aactgaaaga acaccagcac cgatacacga aacgaaaccc aaatcgtcaa
     9541 cgtaatgacg ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt
     9601 atcaataatt cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag
     9661 ataaatgcca aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg
     9721 aatgatatgt ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa
     9781 ggcagaaacg gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat
     9841 caccgcgatg agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag
     9901 tactttatct gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa
     9961 taccgcagaa aagaagcaaa atggaattgt aagcaggaca tcgcggaaaa agacggaggc
    10021 ttgcgggaag gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac
    10081 gacaataatc cctactttga ccaccaccat tggcccggaa atcttaaaca gtaatcgttc
    10141 accaccagac gcaatcgcta ccagtacggc gaaaatagcg actttataaa gtagagattg
    10201 tgaaagatcg gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac
    10261 ggagagagag tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa
    10321 aattccccag ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc
    10381 gctttcagaa agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat
    10441 gatcgctgcg gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac
    10501 ggttccggca ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt
    10561 aaacggcaat ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga
    10621 aagaagtcgg cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag
    10681 tcgaatacgc agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc
    10741 gcaattctac aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc
    10801 aactattgca aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg
    10861 tgctgacagt aaaaagcaat cgtaaaatca gcggcaagcc gaaagggttc cggtgaggcg
    10921 caatgttgcg ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca
    10981 cgttgcaggt atgcacctga caacatcacg ggggctaatc ttgaacccaa acaactcaag
    11041 aatagccgcg aaacgttggc attacaacac aggcggctat atgacgctcg cagaactggg
    11101 catggccttc tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat
    11161 cgtgaactgg ctgaataagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg
    11221 caatttgcgc ccggatcagg ccgcagaggt taaactctgc ggcctttttc gtgcttactg
    11281 cgggtaaggc acccagtcgc cgccgttcag gcgaacgtac ggtttatcct ggtattgaat
    11341 aactactgca tttgagttct cggagaccgg tgctgtttgt ggcaacccac tggtgagttt
    11401 tttccagtca acattgtctt cggtgaaaat cttgccatca agaacgcgaa ccaccagatc
    11461 ggagatagcc aggaagctgc tcggttggtc gatgacaatc ggtgccccct gatgtggtgc
    11521 cttcatgccg aagaatttca ccccaacggg gacgtctgtg atagacgggc tagggatatc
    11581 acgtaggcca gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac
    11641 gaccaccatc actttacgac ccgatttctc cagttcagta aagaaggcgt ccagttcatc
    11701 aaagaatttc tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg gataatggtt
    11761 gccgtcatgc agtggaagcg tgttgtagaa cgtggcacta cggctgtttt tatctttttc
    11821 ggtaacgtcc agccagcggt taagcacggt ggtatcgtca taaaccggcg aaccatcaaa
    11881 gcccagcaaa ataaccggca gatttgtttg atccatcaat tcagtctgca tgccgccatt
    11941 ttcgcgaact tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca tcaggtgctg
    12001 ggtaaagccc agtttcgaaa ggttatcaaa cagatagcag tcgttatttg ccggttgata
    12061 cagattagtg tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc
    12121 actgtaggag gtggcggagt taaagttctt gaactcaata tcgaaatgcg accacagtgg
    12181 atgcgacatc aacccggcgg cttctatatc cgaccaggaa agcgaacaga tgttaatcac
    12241 cagtagttca aatggctgag catcagcggg cagcgaagac gggaaggtcg atttacgttt
    12301 cgcctccgcg ttatagaaat tattcagcca ggcgttaagg ttcgccgttg ttggcggtgt
    12361 agtttgtgcg ggcatatcac ccactaccgg tgcgccaccc gtcgtctcaa cggttgccgc
    12421 tgcgttacca cccgtcgttg ttacagtggt cgtcggttgt ccggctggcc acaaggagaa
    12481 acttggtccc gccagggtaa gtacgttcag ccatagcagt atggcaacca caaaaacggt
    12541 aatgcgaatc cattgtgaca ggaataacca ggccactaat aaaacaaaaa tggccccaat
    12601 catctgccag ttaataaagc gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc
    12661 cacctgcgaa ccctggctca ttatgctttc cgggccaggc aaccaggtgt catgccagaa
    12721 caaagcaaag ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc tgtagcgcgg
    12781 aatgggcatc agcagaaacg cggcaaacac caaattgagg agcggatgga agttaagata
    12841 tcccgcccac aacaggccga acttaaccag aaaatagaag ttccagccgg aaaggccgcg
    12901 ccagtattgc cagagggaag aaggcatggc ggtattttgc gtaaattgag tcattttttg
    12961 gttgccctgg ctttttccgt gcggcgtaat gtcccggccg gtttaacata acgaggttta
    13021 gcaaagaaca aacgtaaggt gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc
    13081 agcgggaaaa atatcagtgc gcaaacgaca ataatttcaa tgatatcgct gatggtcatc
    13141 atgatgagcg ctccacagca tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt
    13201 ggcgaccatc gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca
    13261 tgccccattg ttctggggca agcaggcgca tctgcaccag ctcggcactg atttgatcat
    13321 cttcaaacca gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat
    13381 tcaacgcggt atccagatcg ttaatccgac agaatgagag aaacagcacc agccgattac
    13441 cgccaatggt catgatatcg ccggtacggt tagggcgaca cagcgtcagg gcttgttcaa
    13501 cgcggatacc cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg gcaggtaata
    13561 gagggttatt catcatgttg ttgacggcat tacagaacac atcccacttc tggaaaccac
    13621 gcagtttgag cggctgggtc attgacagca aggtagtgat atcttccggc acatagcgac
    13681 taaacttctg cccttgcacg ctttcgatca tcgtcagaca acgggagagt ggcgcattcc
    13741 acggaataac catatttgca ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc
    13801 tagcggtatt ttcccgcacg aggattttca tcgcactacc gcgctggcga cgcagggtat
    13861 gaatgctgcg ggccagtggc tcgatttgcg cattttgctg taaagaaaag accaccgtcg
    13921 ccgcctgagc ggtacgggct tcattgaaca ggacttcgtt attgttgaac agttgccagt
    13981 gttccgatag cggcggcgca ccttccagta cagcaacatt actcagaatg cgtttttcgt
    14041 cgctgcgtgg ttggatctcc gcctcttcgc tttgaactaa tgtccagata ccattttgtt
    14101 gctgaacgct aagctgctga cgggcgctga cccctttttc gttgcaccag aaggcaatat
    14161 ccagcaaatg ttggtcaccc tgaaaacgca aactggcaag accaaaaagt gaacggtatt
    14221 cctcaagcaa caatgaaaat tgtttatcgt tattatttcc gggattaatt accaaaagcg
    14281 aacaatgggt taacctgctc catttattca ttttatccaa ccatgagcga agccgctcgg
    14341 caggaatgtt ttgccatgcg ttatttgcgc aaacaagaat aaaaaggtaa ttatggggat
    14401 caatagaaca ctgcaaatca cgggtcaaat agtatagacc tttttcatga tttagcattg
    14461 aaaataattt tattttttcc ggcccttgat aatcatctaa ttgaaagatt ttcgccggat
    14521 cgctgtccat gctaatgacc gcgacgtgtg cggtttcagc ctgggatgca attgtttgat
    14581 tcgccagact gatagcatct tcatggcgat cgacgttaaa ccaccagacg ccgcctgctg
    14641 gcatatgtcg cagctcatcc cataatgatg agataccgat agagaataca gggcccacaa
    14701 tgtccctcat gtcaccattt tgtctgatta tcagtttact agcgaaagct aagaaataaa
    14761 cctaccattg aaattaaaac ttatcagatt tagcatgtga atcaatttag aaggtcacaa
    14821 gacgtgtgat tttagtcttt tcgtttttaa ctttttctgt tgcgtaacgg aaagtcaaaa
    14881 agtgagcaaa ttcccgtctc gccgtaaaca ataaaccggc gaaatgctca cggttagaat
    14941 tgtctcatga acggtacggt tatttcatag ggatcaagca aaatgaataa caatgaacca
    15001 gatactctgc ctgatcccgc gataggctat atcttccaga atgatattgt ggcgttaaag
    15061 caggcatttt cactgcctga tattgattat gccgatattt cccaacgcga acagttggcc
    15121 gcggcattaa aacgctggcc gttgctggca gagtttgcgc aacaaaagta ggggattggt
    15181 gaatggccgt actgggattg cagggggtgc ggggaggcgt ggggacaaca accatcaccg
    15241 ccgcattagc ctggtcatta caaatgttgg gagaaaatgt cctggtggtc gatgcctgcc
    15301 cggacaactt gttgcgcctg tcatttaatg ttgattttac ccaccgtcag ggctgggcca
    15361 gagcgatgct ggatggccag gactggcgtg acgctgggtt gcgctacacc tcgcagctcg
    15421 atttgctgcc ttttggtcag ttatccattg aagaacaaga aaatccacag cacaggcaaa
    15481 cccggctgag cgatatttgc tccggcttac agcaactaaa agccagcggg cgttaccagt
    15541 ggattttaat cgacttaccg cgtgatgcct cgcagataac ccaccagctg ctgagtttgt
    15601 gcgatcactc gctggcaatc gtcaatgtgg atgccaactg ccatatccga ctgcatcagc
    15661 aagcgctgcc ggatggcgca catattttga ttaatgactt ccgtattggc agtcaggttc
    15721 aggacgatat ttaccagctt tggttgcaaa gccagcgccg attactgccg atgctcattc
    15781 atcgtgatga agcgatggct gaatgcctgg cggctaagca accagtaggt gaatatcgca
    15841 gtgatgcgct ggcggctgaa gagatactga cgctggcgaa ctggtgcctg ttgaactact
    15901 ccgggctgaa aacgccagtc gggagtgcat catgagtatc ctgacccggt ggttgcttat
    15961 cccgccggtc aacgcgcggc ttatcggggg ttatcgcgat tatcgtcgtc acggtgcgtc
    16021 ggctttcagc gcgacgctcg gctgtttctg gatgatcctg gcctggattt ttattccgct
    16081 ggagcacccg cgctggcagc gtattcgcgc agaacataaa aacctgtatc cgcatatcaa
    16141 cgcctcgcgt ccgcgtccgc tggacccggt ccgttatctc attcaaacat gctggttact
    16201 gatcggtaca tcgcgcaaag aaacgccgaa accgcgcagg cgggcatttt caggtctgca
    16261 gaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg ttagccataa
    16321 aacacagcat cttgatgaga aaaaagagct cggtcatttg agtgccgggg cgcggcggtt
    16381 gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg ttactcaacc
    16441 gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggtgtag cgctgatcgt
    16501 acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc
    16561 ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc cggtcagcct
    16621 ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg
    16681 ctacttccag gtcgtatggc cgctgaatcg tcagccggtg ccattgccga aagatatgtc
    16741 gctgtggccg tcggtggata tctttgtccc gacttacaac gaagatctca acgtggtgaa
    16801 aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga acatctggat
    16861 cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata
    16921 tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg cgctgaaata
    16981 tgccaaaggc gagttcgtat cgattttcga ctgcgaccac gtaccaacgc gatcgttcct
    17041 gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc agacgccgca
    17101 ccacttcttc tcgccggacc cgtttgaacg caacctgggg cgttttcgta aaacgccgaa
    17161 cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg acgccacttt
    17221 cttctgcggt tcctgtgcag taattcgtcg caaaccattg gatgaaattg gcggtattgc
    17281 tgtcgaaacg gtaaccgaag atgcgcacac gtctctgcgc ctgcaccgtc gtggctacac
    17341 ctctgcatat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca
    17401 tatcggtcag cgtattcgct gggcacgcgg gatggtgcaa atcttccgtc tcgataaccc
    17461 gcttaccggt aaagggctga aattcgccca gcggctgtgc tacgtcaacg ccatgttcca
    17521 cttcttgtcg ggcattccac gactgatctt cctgactgcg ccgctggcgt tcctgctgct
    17581 tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctgttcgtgc tgccgcatat
    17641 gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact ctttctggag
    17701 tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg tggcgctgat
    17761 taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg aagaagagta
    17821 cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctgctcaacc tggtgggtgt
    17881 tgcggtcggc atctggcgct acttctatgg cccgccaacc gagatgctca ccgtggtcgt
    17941 cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg cggtatcggt
    18001 agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg cggcaattgc
    18061 ccgcgaagat ggtcacctct tttcgtgtac cgttcaggat ttctccgacg gtggtttggg
    18121 gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc tgttgcttaa
    18181 acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt
    18241 tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc agtgtacgtt
    18301 tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata agccgctgga
    18361 aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg cggagtttgc
    18421 gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct gggttgtatc
    18481 gtttattctg cgccgcccgg agcggagcga aacggcacaa ccatcggatc aggctttggc
    18541 tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca gtggctatgg
    18601 ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa ccactgatca
    18661 atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg caagtgatgc
    18721 cgggcgtgca gggcgctgat gcgccagtcg tggcgcagaa cggtccttcg cgtgatgtga
    18781 agctgacctt tgcgcaaatt gcgcctccgc cgggcagcat ggtgctacgt ggcattaacc
    18841 cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag gcgatgctca
    18901 acctcgaata catgccatcg ccatcgttac tgcctgtcca gtcgcagtta aggtttatct
    18961 caatgatgag ctgatgggcg tactgccagt gaccaaagaa cagttgggta aaaaaacgct
    19021 ggcgcaaatg cccattaacc cactgtttat taccgacttc aaccgtgtgc ggctggagtt
    19081 tgtcggccat tatcaggacg tgtgcgaaaa cccggccaga accacgcttt ggctggatgt
    19141 tgggcggagc agtggactgg atctgaccta tcagaccctg aatgtgaaga atgacctgtc
    19201 acacttcccg gtgccattct ttgacccgcg cgataaccgt actaacacct tgccgatggt
    19261 ctttgcgggt gcgccggatg ttgggctgca acaagcatct gccattgtcg cctcgtggtt
    19321 tggttcgcgt tctggctggc gtgggcagaa cttcccggtg ctctataacc aactgccgga
    19381 tcgtaatgcc attgtctttg caaccaacga caaacggccg gacttcctgc gcgatcatcc
    19441 ggcggtaaaa gccccggtga ttgagatgat caaccatccg cagaatcctt acgtcaaact
    19501 gctggtggtg tttggtcgtg acgacaaaga cctgttgcag gcagcgaaag gtatcgctca
    19561 gggtaacatt ctgttccgtg gtgaaagcgt ggtagtgaat gaagtgaaac cgctgctacc
    19621 gcgtaagccg tacgatgcgc cgaactgggt acgtaccgat cgtccggtca catttggcga
    19681 actgaaaacc tatgaagaac agttacaatc cagcggtctt gagccagcag cgattaacgt
    19741 ttcgctaaac ctgccgccgg atctctacct gatgcgcagt accggcattg atatggatat
    19801 taattaccgc tacaccatgc cgccggtgaa agacagttcg cggatggata tcagcctgaa
    19861 taaccagttc ctgcaatcct tcaacctgag cagcaaacag gaggcgaacc gcctgctgct
    19921 gcggattccg gtattacaag gtttgctgga tggcaaaaca gatgtctcta ttccggcgct
    19981 gaaactgggc gcgaccaacc agctgcgctt cgactttgag tatatgaacc cgatgccggg
    20041 cggttcggtg gataactgta ttaccttcca gccggtgcag aatcatgtgg tgattggtga
    20101 cgactccacc atcgacttct cgaagtatta ccacttcatc ccgatgccgg atctacgcgc
    20161 ctttgctaac gcgggcttcc cattcagccg gatggcggat ctgtcgcaaa ccatcaccgt
    20221 gatgccgaaa gcgcctaacg aagcacagat ggaaacgttg ctgaatactg ttggttttat
    20281 cggcgcacag acgggcttcc cggcgattaa tctgacggtg accgatgatg gcagcaccat
    20341 tcagggcaaa gatgccgaca tcatgatcat cggtggtatc ccggacaaac tgaaagacga
    20401 taagcagatc gacctattgg tgcaggcgac cgaaagctgg gtgaaaacac cgatgcgcca
    20461 gaccccgttc cccggcattg tgccggacga gagcgatcgc gcggcagaaa cccggtcaac
    20521 gctgacctct tccggtgcga tggcggcggt gattggcttc cagtcgccgt ataacgacca
    20581 gcgcagcgtg attgcgctgt tggcagatag cccacgcggt tatgaaatgc ttaacgatgc
    20641 ggtgaacgat agcggcaaac gcgccaccat gttcggttcg gtcgcggtga tccgcgagtc
    20701 cggtatcaac agcctacgtg ttggcgacgt ttattacgta ggtcatctgc cgtggttcga
    20761 gcgcgtgtgg tatgcgctgg caaaccatcc gattctgctg gcggtgctgg cggctatcag
    20821 tgtgatattg ctggcatggg tactgtggcg tctgctgcga attattagtc gtcgtcgtct
    20881 taacccggat aacgagtaat tgaagatgaa tgtgttgcgt agtggaatcg tgacgatgct
    20941 gttgttggct gcctttagtg ttcaggcagc ctgtacctgg cctgcctggg agcagtttaa
    21001 aaaggattac atcagtcagg aagggcgcgt catcgacccc agcgacgcgc gcaaaatcac
    21061 cacctccgaa gggcaaagtt acggtatgtt ctttgccctg gcggctaacg accgtgcagc
    21121 tttcgataat attctcgact ggacgcagaa caatctcgct cagggttctt taaaagaacg
    21181 tttgcccgcc tggctgtggg gcaagaaaga gaacagtaag tgggaagtgc tggacagcaa
    21241 ttcggcctcc gatggtgatg tctggatggc ctggtcgttg ctggaggcgg ggcgtttgtg
    21301 gaaagagcag cgttataccg acatcggcag cgcgttgcta aaacgtatcg cgcgggagga
    21361 agtggtgacg gtgcctgggc tgggttccat gttgttaccg ggcaaagtgg gttttgctga
    21421 ggataacagc tggcgtttta accccagcta cctgccgccg acgctggcgc agtatttcac
    21481 ccgctttggc gcgccgtgga ctacgctgcg cgaaaccaat caacgtttat tgctggaaac
    21541 cgccccgaaa ggcttttcgc cagactgggt gcgttatgag aaagacaaag gctggcagct
    21601 aaaagccgaa aaaacattga tcagcagcta cgacgccatc cgcgtttaca tgtgggtagg
    21661 catgatgcct gacagcgatc cgcagaaagc gcggatgctc aaccggttta aaccgatggc
    21721 gacattcact gagaaaaacg gttatccgcc ggaaaaagtg gatgtggcta cggggaaagc
    21781 gcagggtaaa ggaccagtcg gtttttctgc cgccatgctg ccctttttac aaaaccgcga
    21841 tgcgcaggcc gttcagcgtc agcgcgtggc cgataacttt cccggcagcg atgcctatta
    21901 caactatgtg ctgaccctgt ttggacaagg ctgggatcaa caccgtttcc gcttctcgac
    21961 aaaaggtgag ttattacctg actggggcca ggaatgcgca aattcacact aaacatattc
    22021 acgctttccc tcggtctggc cgtcatgccg atggtcgagg cagcaccaac cgctcagcaa
    22081 cagttgctgg agcaagttcg gttaggcgaa gcgacccatc gtgaagatct ggtgcaacag
    22141 tcgttatatc ggctggaact tattgatccg aataacccgg acgtcgttgc cgcccgtttc
    22201 cgttctttgt tacgttaggg cgatattgat ggcgcgcaaa aacagctcga tcggctgtcg
    22261 cagttagcgc cgagttcaaa tgcgtataaa tcgtcgcgga ctacgatgct actttccacg
    22321 ccggatggtc gtcaggcact gcaacaggca cgattgcagg cgacgaccgg tcatgcagaa
    22381 gaagctgtgg cgagttacaa caaactgttc aacggtgcgc cgccggaagg tgacattgct
    22441 gtcgagtact ggagtacggt ggcgaaaatt ccggctcgcc gtggcgaagc gattaatcag
    22501 ttaaaacgca tcaatgcgga tgcaccgggc aatacgggcc tgcaaaacaa tctggcgcta
    22561 ttgctgttta gtagcgatcg ccgtgacgaa ggttttgccg tcctggaaca gatggcaaaa
    22621 tcgaacgccg ggcgcgaagg ggcctctaaa atctggtacg ggcagattaa agacatgccc
    22681 gtcagtgatg ccagtgtgtc ggcgctgaaa aaatatctct cgatctttag tgatggcgat
    22741 agcgtggcgg ctgcgcaatc gcaactggca gaacagcaaa aacagctggc cgatcctgct
    22801 ttccgcgctc gtgcgcaagg tttagcggcg gtggactctg gtatggcggg taaagccatt
    22861 cccgaactac aacaggcggt gcgggcgaac ccgaaagaca gtgaagctct gggggcgctg
    22921 ggccaggcgt attctcagaa aggcgatcgc gccaatgcag tggcgaatct ggaaaaagcc
    22981 ctcgcactgg acccgcacag cagcaacaac gacaaatgga acagtctgct gaaagtaaac
    23041 cgctactggc tggcgatcca gcagggcgat gctgcgctga aagccaataa tcctgaccgg
    23101 gcagaacgcc tgttccagca ggcgcgtaat gtcgataaca ccgacagtta tgcagtgctg
    23161 gggctgggcg atgtggcgat ggcgcgaaaa gattatcccg ccgccgaacg ttattatcag
    23221 cagaccttgc gtatggacag cggcaacact aacgccgtgc gcgggctggc aaatatttac
    23281 cgccagcaat cgccagaaaa agctgaagcg tttatcgcct cgctctctgc cagtcagcgg
    23341 cgtagcattg atgatatcga acgcagcctg caaaacgacc gtctggcaca gcaggcagag
    23401 gcactggaaa accagggcaa atgggcgccg gcggcagcac ttcagcggca acgactggcg
    23461 ctggaccccg gcagcgtatg gattacttac cgactttcgc aggatctctg gcaggccgga
    23521 caacgcagcc aggccgatac gttaatgcgc aatctggcgc agcagaagtc gaacgacccg
    23581 gagcaggttt acgcttacgg gctgtacctc tctggtcatg accaggacag agcggcgctg
    23641 gcgcatatca acagcctgcc gcgagcgcag tggaacagca atattcagga gctggttaat
    23701 cgactgcaaa gcgatcaggt gctggaaacc gctaatcgcc tgcgagaaag cggcaaagaa
    23761 gctgaagcgg aagcgatgct gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg
    23821 gctgactggg cgcagcagcg acgtgattac accgccgccc gcgctgcata tcagaatgtc
    23881 ctgacgctgg agccaactaa cgccgatgcc attctggggc tgacggaagt ggatattgct
    23941 gccggtgaca cagcggcggc acgtagccag ctggcgaaac tgcccgctac cgataacgcc
    24001 tcgctgaaca cacagcggcg cgtggcgctg gcgcaggcgc agcttggaga taccgcagcg
    24061 gcgcagcaga cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa
    24121 agcgcgatgg tgctgcgcga cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag
    24181 gcgctggaaa cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag
    24241 gataacgaca cattcacccg actgacccgt aacgacgaga aagatgactg gctgaaacgc
    24301 ggcgtgcgca gcgatgcggc gggcctctat cgccagcagg atcttaacgt caccctcgag
    24361 cacgattact ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc
    24421 atgttgcagg tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc
    24481 aatatgaacg tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc
    24541 acctgtacat tacaggactg tagtggcaac cgcagccagt cggactccgg tgccagcgtg
    24601 gaggtcggct ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc gatgggcttc
    24661 aacgtggtgg atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac
    24721 accgttaacg cccaccgtcg gcccatctcc agttctttgc tggcctttgg tgggcaaaaa
    24781 gactccccga gcaataccgg gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta
    24841 agtctgagct acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag
    24901 ttaaccggta aaaatgtcga agataactgg cgcgtgcgct ggatgacggg ctattactat
    24961 aaggtcatta accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat
    25021 tacgacaaag atctgagtgg ctactcactc ggtcagggcg gttactacag tccgcaggaa
    25081 tacctgtcgt ttgctatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag
    25141 ctgggggcgt ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg
    25201 atgaatctga tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc
    25261 agcagtcagg gcttcggcta cacggcgcgg gcattacttg aacgacgtgt tacttccaac
    25321 tggtttgttg gcacggcaat tgatatccag caggcgaaag attacgcacc cagccatttc
    25381 ctgctctacg tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag
    25441 ccgctgatac cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc
    25501 gatcgggtat actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt
    25561 cgctcgttaa caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt
    25621 attttttgca ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg
    25681 caactggaaa gcattgctcg ctctgtacgc gaacccttat cttcagctat tttgaaaggc
    25741 gatattcccg aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt
    25801 gccgatgtag tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgc
    25861 ccggtgccgg taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtt
    25921 tactcgctcg aacgtccggc aaacccgcag ccaattgcct atctggtact acaggcggat
    25981 tccttccgta tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta
    26041 cttttgtcgc ttatcctgac cgtcgccatc agctggtgca ttaaccgcct gattttgcat
    26101 ccgttacgca atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac
    26161 caactggcat taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac
    26221 aacctcaacc agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc
    26281 ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc
    26341 gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg tgatactgcg
    26401 ggcgtgctga aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa
    26461 tcggtactgt cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt
    26521 gccaacggtg tacaggaacc gtggcacgca atcaccttag gtcagcaagt gctcactatc
    26581 atgagcgagc gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattggcgtg
    26641 gcgatgttct acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca
    26701 tttaccgctc gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa
    26761 gccgcccaga agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag
    26821 tttgctattt ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa
    26881 gtgttactgc gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat
    26941 cgcattgagt gctgtgggct gatggttacc gtcggtcact gggtgctgga agagtcctgt
    27001 cgattgcttg cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct
    27061 gcgctgcaac tgatgcaccc aaatatggtg gcggatatgc tggaactgtt aacccgctat
    27121 cgcattcaac cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct
    27181 catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat
    27241 gatttcggca tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc
    27301 gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt
    27361 gctgcaatta tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag
    27421 actgaagcac aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt
    27481 tttgctcgcc cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta
    27541 ccccaaactg attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt
    27601 taaccttttt gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt
    27661 ttaaggccgc aggtacctca taaccttaca agacctgtag ttttactaaa ggacacccta
    27721 tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta
    27781 ttctccttgg ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct
    27841 tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca
    27901 ttgcgggcat ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact
    27961 ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc
    28021 ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg
    28081 ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggtgagcg
    28141 tcattggcgc atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg
    28201 gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt
    28261 tctcgcaggt catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt
    28321 tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc
    28381 agctgattat ctgtttctat attacctgta tcctgtttgt ggtgctggta ttgggttcaa
    28441 tcgctaaagc gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc
    28501 tgattgtact ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg
    28561 agaaactcgg ctgccgcaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta
    28621 accttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta
    28681 acagtcagat ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta
    28741 aaggggcggc aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg
    28801 tgggccattt gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag
    28861 aagctcgtgc gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt
    28921 gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg
    28981 gcaaaacgca cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag
    29041 tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg
    29101 tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta
    29161 acgtcattta gactgactga caacgggcag ttttttatta ccaggatagt tgatcagggg
    29221 ttcacatgca gggcacaaaa attcgacttt tagcgggcgg tttgctgatg atggccactg
    29281 ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt
    29341 ccaacggttt acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa
    29401 ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc
    29461 acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt
    29521 cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg
    29581 acaccacgct gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc
    29641 tctcttatct ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg
    29701 cgctgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc
    29761 gctatcgtct gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac
    29821 ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga
    29881 cgctactggt ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt
    29941 ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc
    30001 gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt
    30061 gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg
    30121 acctggcccg cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtaacagca
    30181 aagacatcgg tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca
    30241 acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg
    30301 cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga
    30361 aactggagct gcagaaactg tttgccgcct atgcgcgggc tgataccgat attctgatgg
    30421 gtcagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga
    30481 aactgcggca ggatttcctt aacagcctga cggtggagat gttaaatcag gatctgcgtc
    30541 agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta
    30601 acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatcgact gctgctgccg
    30661 ccacctctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt
    30721 aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag
    30781 gggtaacact ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg
    30841 ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg
    30901 cgttttccgc actgccgccc gtcaaacgta ccgccagata accggcgctg aaagagtcgc
    30961 cagctgcggt ggtatcgatc actttttctt tcggcagttt caccgccgga acatccacta
    31021 acccttcgcc agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt
    31081 tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg
    31141 cgtcttcatc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgttggtaca
    31201 cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca
    31261 cttttccgcc gttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg
    31321 ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt
    31381 cgcaaatcgc cgtagactgc tcactctcca gccagaattt ggcggcggct tcgttccgcc
    31441 agtagtagaa cgtacgctcg ccggtgctgt cggtttcaat gtagtaaagg cccggcagac
    31501 ggttttccat ccgttgggtc agggaaatat caacgttctc gccgtgccag gcgtccagca
    31561 tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag
    31621 gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct
    31681 taacgtccgc gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt
    31741 tggacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt
    31801 gctggtcaac aatattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga
    31861 aagattccgt tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttac
    31921 gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat
    31981 gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga
    32041 acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg
    32101 cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca
    32161 acgcctgccg ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt
    32221 gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttatagagc acggcctgct
    32281 ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct
    32341 gcgccaaatt gagcgtcttc cctggctgcg tttcgaactg gtggagcata tccgtctgcc
    32401 gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg gtgattttgg
    32461 taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca tcaaaatcgc
    32521 gcgagaactg tttgtgatgc tgcgtcagtc gccggaagga cgcacactct tttctcagct
    32581 tttacatcta atgaatcgct attgtcgcgg ggtgattgtt gagggcgtag aaacgccgga
    32641 agagtggcgt gatgtacaga actcgcccgc attcgccgca caaggctggt ttctttcacg
    32701 cccggcaccg atagaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc
    32761 cgcctggctg gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag
    32821 caaggcaggc aaaataactg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc
    32881 gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt
    32941 ctctgcggag ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg
    33001 gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga
    33061 catcattctt ggcaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt
    33121 agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc cgtggatcaa
    33181 gctcgaaaag cccgacgcgc gcctgattcg cctctccgaa aagaacaata actggacgtt
    33241 taatctcgcc aacgatgata acaaagacgc gaatgcaaag ccgtcggcat ggtcgtttcg
    33301 gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc
    33361 ggatctggaa atttttgttg atccgttagg caagccgctg ccgttcagcg aagttactgg
    33421 atcgaaaggt aaagcggata aagaaaaggt gggcgattac gtttttggcc tgaaggcgca
    33481 gggacgttat aacggtgaac agctcacggg tacgggaaaa ataggcggta tgctggcgct
    33541 gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc cgttcaggta atacccgtgt
    33601 tgcttttgat ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt tacggcttaa
    33661 attttctggc gattcactgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac
    33721 cccgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt
    33781 ctttgattat cgcggtttta atgggcgaat tggcgatagc gatatccacg gttctctggt
    33841 ctacaccacc ggaaagccgc gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg
    33901 gctggcggac ctgggaccgt tgattggcgt tgattcgggg aaaggggcag aaaagtcgaa
    33961 acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta
    34021 tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaaggccg
    34081 acgtatcgag catggcagta gcctgccgat tagcgatctt tctactcata tcatcctcaa
    34141 aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca gcattgcggc
    34201 gaatatccat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg
    34261 tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg atgcagaaga cgctggggga
    34321 aatgaacggt gacgcggaac tacgcggtag cggtaactcg gtggcggcgc ttttaggcaa
    34381 cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga
    34441 gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg acgatgaggt
    34501 gcgggtgaac tgcgcggcgg cgaatctgaa tattgccaac ggcgtagcgc gcccgcagat
    34561 ttttgctttc gatactgaga acgcgttgat taatgttacc ggcacggcaa gttttgcttc
    34621 ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg
    34681 ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccggacc
    34741 gctgattgcc cgtggtgccg ttgcggcggc actggcaacg ctggtaacac cggcggcggc
    34801 gttattggca ctgatctcac cttccgaagg ggaggctaat cagtgtcgga cgatattgtc
    34861 gcagatgaag aagtgatgag gttggattgt gtttgctggt gagttcggga gcagcgtgtt
    34921 cccctcaccc taatcctctc cccaaagggg cgaggagact gttttgtgcg tgttgaaaca
    34981 acgagtaccg cttttccgtt cactttctgg gggagggctg ttatgttcgc gatataacat
    35041 ctcgctccgc ttttctccct ctccctttca gggagagggc tggggtgagg gtggctgccc
    35101 acacgatact tacaacgact gatgttgcgt ctcatgggtc agcagcaggg cgattaacgt
    35161 caagccagcc atcgccgcca gatataaccc caccgcacct aacccgtagt tagtctgcaa
    35221 ccaggctgcg atatacggcg caacggaagc cccgagaatc gacgatacgt tgtaagagaa
    35281 cgatgctccg gtgtaacgca cttctgtcgg gaacagctct ggtaacagcg cgcccatcgg
    35341 accgaaggtc agccccatca gacttaaccc cagcagcagg aaggcaaaaa ccagaatcgg
    35401 gttgccagaa ccgagcaaag ggttaaaggc gaatagcgcg aacaggatga gcagcgtggt
    35461 gatgattacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccag cgactggcac
    35521 catcacgcca aaaccaataa ctgccatcat caacatccac aacacttcgt tacgcggcag
    35581 gccaagccca actggcgcgg cggcggtact aaaggtcatg gagtagaccg tcatgatgta
    35641 aaacagcgta taggttgcca gcataatgaa cgtacccagt acggttacgc gaacatgttt
    35701 ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga ctttctcaaa
    35761 caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca gcaccgccga
    35821 gaagataaat ggcacacgcc agccccagct cataaactgc tcgtcggtca gtagccagga
    35881 aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccca gctgcgggaa
    35941 ggagccgtac aatgcacgtt tgcgcggtgg ggcgttttca gtcgccagca gcgccgcgcc
    36001 gccccattca ccacctaagc ccagaccctg accaaatcga gccagcgcca gcagcagcgg
    36061 ggcgaaaata ccaatcgtgg catagcctgg cagcagacca atcaccacgg tcgaaatccc
    36121 catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc caaaatgacc
    36181 aaaaacggca gagccaatgg ggcgcgcaac gaaggcgatg gcgaaggtgg cgagcgactg
    36241 tagcgttgct gccgtaggat cgccctgcgg gaagaagata tgcggaaaca caataacggc
    36301 cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggct gtgccaatga gagaggcgac
    36361 aaggacttta ttacgcgagt tgatcggcgt gtattcttgc tcgtggtcga gtgttgtggc
    36421 tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt acgcacagca
    36481 aaagcgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg caaaaagggg
    36541 ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt tataaatagt
    36601 ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg ctggggttat
    36661 gagttagaat gaaattgttt gctcccaaat agatggctga agaggctctg gggacgagtt
    36721 tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta tccggcctac
    36781 ggggagccag gccgaataaa atcgtatgcc ggataaggcg tttacgccgc atccggccta
    36841 ttttaaggct gcgttttccc tagcattcgc gggtcgtctt tatattcggc ggtggcaatc
    36901 cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat cagccccagc
    36961 acggaaccaa atgctgcgcc agagggggat ttcatcagcg atggcagggt gtaggtcatc
    37021 acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat cagggctttt
    37081 ttgcgcggac ggtggcgcgg caaccgccag aagatccaga agaaaagcag atagttggcg
    37141 aagatggaaa tcgccaggcc aatcaatcgc cacgtcggtt tcagccactc aatgctgttc
    37201 aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt gatcgaaagc
    37261 gtcacaatca gcgcaatcaa caaaccaatc agtgaaataa aatcacgcag atattttacc
    37321 cagaacttct cctgatcttg cggcgagcgt tcccagacat cacgcgactg ggcacgaatc
    37381 gcttcacgca ggttacccat ccagttgatg ccggaataaa gtgccaccgc caggccgaca
    37441 agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa cgtggcggct
    37501 agtgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag caacatcgga
    37561 tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg gatcatcgac
    37621 aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc gttaaagcgt
    37681 tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt ggactttttc ggcagtggtg
    37741 gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc caactgctta
    37801 atcggctcga gctccagatc ctgggtggga cgtttgatct cgttttcctg cgtcatccgc
    37861 tcttttgtcc ttctgtcgtg gtcattctta aaaagtatag tcggtcagtc caggtgctct
    37921 ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag ctcccgacgc
    37981 tgcgggtaaa ccagcgaaag ggagagcggc tcggcacggt agccaggtaa tacttcaata
    38041 agccgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat aatccccaga
    38101 ccggcaagac cggcggcgag atagttttcg ctactattta ccgtcaacat gccgccagac
    38161 ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg tacacccagg
    38221 tgcggtgtat aacgcactat tgcgtgtgaa gtcagatcat cggggctttg cggataacca
    38281 aagcgcgtca gatagtgcgg actggcacag ttgaccatgg tcagtttgcc gaggggacgg
    38341 gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa accatcatga
    38401 agaatatcta ccggacggtc atggctactc agttccagct caattcccgg atgcagatag
    38461 agaaattccg acaggcgcgg cagtaacagg tttttcgcga ttcctggcgg gatgtcgatg
    38521 cgtaatttac cgctgatact ggtggcatcc tgttgaaaca gaccgtccag ttcgttgaga
    38581 ttactcaaca catcttttgc ccgttgataa taggtcatgc cttctggcgt gagtttgacc
    38641 cgtcgcgtgg tgcgctggag aagctgggtg ccaagttgat gttccagtgc ctgtatctgg
    38701 cgcgaaacac ttccctttgg caaagcaaag aaatccgctg cgcgggaaaa actttccagc
    38761 tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt gaccgattgt
    38821 tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttgttttttc taacatctaa
    38881 tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg aaaagggcgg
    38941 aatagcttta tgagaaaggt gaatcccctt aaacagagcg tatttttcag tgattccctt
    39001 taaagtgaat agaggacgta ttggctattt gtgcttattt ttgtctcatt gccatttgtt
    39061 attttggctt gtagtcgcgg aatttcattt ttaattgcat gcgtactaac caccaatata
    39121 aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag gcgtttagct
    39181 gtaactattt atatcggtaa ctattttcta cgcgtcatct ctgtagaatg gccaccgcca
    39241 aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa gtctttttaa
    39301 catattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag tcaatattta
    39361 ttcatggaat gaaaatcagt ttacagcagc gtattccagg ggtgagtatt cagggggcca
    39421 gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta gttatgctcg
    39481 atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg gtgcagtttc
    39541 ctgaggttaa ggtgttaatc acggcgacgg attgcaataa acggtggtta caggaagtta
    39601 tccattttaa tgtgctggcc attgtgcctc gtgattcaac cgtcgagaca tttgcgttgg
    39661 cggtaaatag tgcggcgatg gggatgatgt ttctgcctgg agactggcgg acgacgccgg
    39721 aaaaggacat taaagatctc aaatcgttaa gcgcccgcca gcgggagatt ttaaccatgt
    39781 tagcggcggg cgagtcaaat aaggagatcg gcagagcgct gaatatcagt accggaacgg
    39841 taaaagcgca tctggaatcc ctgtatcgcc gtctggaagt gaaaaatcgc acccaggcgg
    39901 caatgatgtt aaatatctcc tcctgaagag gataatttta cagccagcag gaagttagcg
    39961 gctggctgta aaaatattat ggttcgccgt acaaaccaat taaacggcgt accacaccgt
    40021 tagtccagcc aaacccatcc tgcaacggat actcgccacc gccgccttcg cgtggaacac
    40081 catcggcaat atggtatttt tcgatcagtt tgtgctgttc cagatagaac tgattcaccg
    40141 tcttcagcca gcttcgcgcg atttcatcac ccagaaggtc atcgccgtac attttaaatc
    40201 cctgaatcgc catccattgt aacggtgccc agccgttggg tttatcccac tgttcaccgg
    40261 tttcgtactc gcttgccaga atcccgccag gtgtcagtaa ccgactgcgc acggcatttg
    40321 ccagacgatc ggcctgttca tggttcgcca taccgacata gagtggcaca atggcggcag
    40381 cggaaaacag cgccagttgt tcgcgtcgcc agtcgtaatc gcggtagatg ccgttttcat
    40441 catcccagag gtaacgcttt accgcatcgc gacgggcact ggccttctgg cggaacagtg
    40501 cttctgtctc tttctcgcct ttcagcgccg agatgttggc gatggcgctc tccagtttaa
    40561 acaggaaggc attcagatcg atggggatga actgggtggt acgaatactc gccagacgac
    40621 cagtatcacg cagccaacgg gaagagtaat cccaaccgga ggccgccccc gctcgtaaat
    40681 cgcggtacac ctcgttgggc gggcgaccag aatgtttcgc ggtttcaacg tcctcaagcc
    40741 aggattcgtc acgcggcgtg tcgcgatcgt cccagtaacg gttgagcagc gatccgtccg
    40801 gcatccgcac aacatggcga taggcctgat tagggatcaa cgattctgca ccgtccatcc
    40861 agaaggcata ttccattttc aggtggtcga gatagcggcg cgcaccgcgt acaccatctt
    40921 cttcaaacaa ctccaccatc agcgcaaaaa ccggtggttg cgagcggctc aaataatagg
    40981 tgcggttgcc gttggggatg tgaccgtagt tttcgatcat ccaggcgaag ttatcggcca
    41041 tgcatttcag caaatcttcc cgaccacttt ccgccagccc cagcatggtg aaataggaat
    41101 cccaatagta ggtttcgcta aaacggccgc ccgggacaat atatgactgc ggtagcgcca
    41161 gcagagaaga ccacggaatg tgatcctgtg gttcgcgggt tagcaccggc cacagctggt
    41221 cgatatgctc tttcagggaa ttttgcgggt ccgatacata ctcgctggag tagacctccg
    41281 gcagccagaa gtgattttca acaaacttgc gcaagtcaaa atcacgatga cggcgcactt
    41341 tacggtagcg gattaaaata tccagcgggt ccattttggg tgcgcagtcg ggaaaggttt
    41401 tactgtcggg gaaaattttc gccgactgaa catgctcgaa cagttcgaga tagcgatcgg
    41461 caggcgtcag cgcatcagag gcgggcagcc cttcaatcat ctcgggttcc ggttctgcct
    41521 cgatcatctc atccagtttt aattcatacg ggtccagttc atagcagaga tcgacttcga
    41581 tcatcagttc gtctggatta gggttttgaa ttttctgatt gagcataagg atccaacctc
    41641 cgaaattcgt cgctaattat cggcttttct ttaagggtag tcattcgctc cgcctggtgg
    41701 gcgacaaagc gtgcggtgga tcacgtttat taacttgtca aaaaatataa cgcactaata
    41761 tcaatggaat ttataaaaat acggatttgg tgttgttttt cattttcaga acattcaatc
    41821 ctgctctcgc cgcaatcatg atatgccatt cctgatctgg gtaataggca caggctatct
    41881 tattgatagt ttatattcat gtaattgatt gattcctatc aaaaagcgta tctccctgtt
    41941 ttttatgatg gctttgtctc gcagagacgt ttgcagcatt ctggaaaatt ttctgggtca
    42001 caataactca ccaccgtgag ggaagaataa tgaaaatggt ctcacgtatt accgcgatcg
    42061 gcctggctgg cgtcgcgatt tgctatttag ggttatctgg ttatgtgtgg taccacgata
    42121 ataaacgcag taaacaggcc gatgttcagg catctgctgt cagtgaaaat aataaggttt
    42181 taggctttct ccgcgaaaaa ggatgcgact attgccacac gccttcggca gaattacccg
    42241 cctattatta tattcctggc gcgaaacagt tgatggatta cgacattaag cttggatata
    42301 aatcttttaa cctcgaggcc gtgcgtgcgg cactgctggc tgataaaccc gtttcgcaaa
    42361 gcgatctgaa taagattgaa tgggtgatgc agtatgaaac tatgccacca acgcgttata
    42421 ccgcgctaca ctgggcgggt aaggtgagtg atgaagagcg ggcggaaata ctggcctgga
    42481 ttgcaaaaca gcgcgcggaa tattacgcca gcaatgatac tgctccggag catcgcaatg
    42541 aaccggtgca gcccatcccg caaaaactgc ctaccgatgc gcaaaaagtg gcgttgggct
    42601 ttgcgctgta tcacgatccc cgtttatcgg ctgatagcac catttcatgc gctcattgcc
    42661 atgcgttgaa tgcggggggc gtcgatggca gaaaaacatc gattggtgtt ggtggcgcag
    42721 ttgggccgat taacgcgccg acggtattta actcagtatt taacgttgag cagttctggg
    42781 atggtcgtgc ggcaacattg caggatcagg caggtggacc gccgttgaac ccgattgaaa
    42841 tggcgtcgaa atcctgggac gaaattattg ctaagctgga aaaagatccg cagcttaaag
    42901 cgcagttcct cgaagtttat ccgcaaggtt tcagtggcga aaatattact gatgccattg
    42961 ctgaatttga gaaaacatta attacgccgg attccccatt tgataaatgg ttgcgtggag
    43021 atgaaaatgc gctgacggcg caacagaaaa aaggctatca attatttaaa gataataaat
    43081 gtgcaacttg tcatggtggt attattctcg gcggacgttc ctttgaaccg ttggggctga
    43141 aaaaagactt taactttggg gaaattacgg cggcggatat tggtcgtatg aatgtgacta
    43201 aagaagagcg tgataaattg cgccagaaag tacccggttt acgtaacgtt gctttaacgg
    43261 caccgtactt ccatcgcggt gacgtgccga cgctggacgg ggcggtgaaa ctgatgctgc
    43321 gctatcaggt aggcaaagag ctgccgcagg aggatgtgga tgatatcgta gctttcctgc
    43381 acagtctgaa cggggtgtac acgccgtata tgcaggataa acaataacta atttgatcgc
    43441 ccgaacagga atgtttgggc gatttttatt acgataataa agtctgtttt taatattatc
    43501 atgttaaatg tttatattat aaaaagtcgt ttttctgctt aggattttgt tatttaaatt
    43561 aagcctgtaa tgccttgctt ccattgcgga taaatcctac ttttttattg ccttcaaata
    43621 aatttaagga gttcgaaatg gaccagaagc tgttaacgga tttccgctca gaactactcg
    43681 attcacgttt tggcgcaaag gccatttcta ctatcgcgga gtcaaaacga tttccgctgc
    43741 acgaaatgcg cgatgatgtc gcatttcaga ttatcaatga tgaattatat cttgatggca
    43801 acgctcgtca gaacttggcc actttctgcc agacctggga cgacgaaaac gtccataaat
    43861 tgatggattt gtcgatcaat aaaaactgga tcgacaaaga agaatatccg caatccgcag
    43921 ccatcgacct gcgttgcgta aatatggttg ccgatctgtg gcatgcgcct gcgccgaaaa
    43981 atggtcaggc cgttggcacc aacaccattg gttcttccga ggcctgtatg ctcggcggga
    44041 tggcgatgaa atggcgttgg cgcaagcgta tggaagctgc aggcaaacca acggataaac
    44101 caaacctggt gtgcggtccg gtacaaatct gctggcataa attcgcccgc tactgggatg
    44161 tggagctgcg tgagatccct atgcgccccg gtcagttgtt tatggacccg aaacgcatga
    44221 ttgaagcctg tgacgaaaac accatcggtg tggtgccgac tttcggcgtg acctacaccg
    44281 gtaactatga gttcccacaa ccgctgcacg atgcgctgga taaattccag gccgacaccg
    44341 gtatcgacat cgacatgcac atcgacgctg c
//
LOCUS       DAJVOY010000008        42610 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVOY010000008 DAJVOY010000000
VERSION     DAJVOY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.008"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..450)
                     /locus_tag="OQL90_000432"
     CDS             complement(<1..450)
                     /locus_tag="OQL90_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCS1367322.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGALGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFIRSHHSSLAISCDLSVEIPVKSEADNL"
     gene            complement(419..1627)
                     /locus_tag="OQL90_000433"
     CDS             complement(419..1627)
                     /locus_tag="OQL90_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HCS1367323.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIQE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            complement(1627..3207)
                     /locus_tag="OQL90_000434"
                     /pseudo
     CDS             complement(1627..3207)
                     /locus_tag="OQL90_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(3239..3886)
                     /locus_tag="OQL90_000435"
     CDS             complement(3239..3886)
                     /locus_tag="OQL90_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1367324.1"
                     /translation="MIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNAI
                     SRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILNN
                     YENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEKI
                     KQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDLILEEKLYLSIRIKVLMD"
     gene            complement(3883..4062)
                     /locus_tag="OQL90_000436"
     CDS             complement(3883..4062)
                     /locus_tag="OQL90_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCS1367325.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFFMSEMSKKINSTN"
     gene            complement(4322..5575)
                     /gene="amiC"
                     /locus_tag="OQL90_000437"
     CDS             complement(4322..5575)
                     /gene="amiC"
                     /locus_tag="OQL90_000437"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1367326.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            5807..7138
                     /gene="argA"
                     /locus_tag="OQL90_000438"
     CDS             5807..7138
                     /gene="argA"
                     /locus_tag="OQL90_000438"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1367327.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7200..9026)
                     /gene="recD"
                     /locus_tag="OQL90_000439"
     CDS             complement(7200..9026)
                     /gene="recD"
                     /locus_tag="OQL90_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1367328.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(9026..12568)
                     /gene="recB"
                     /locus_tag="OQL90_000440"
     CDS             complement(9026..12568)
                     /gene="recB"
                     /locus_tag="OQL90_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1367329.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12561..15449)
                     /gene="ptrA"
                     /locus_tag="OQL90_000441"
     CDS             complement(12561..15449)
                     /gene="ptrA"
                     /locus_tag="OQL90_000441"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1367330.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(15625..18993)
                     /gene="recC"
                     /locus_tag="OQL90_000442"
     CDS             complement(15625..18993)
                     /gene="recC"
                     /locus_tag="OQL90_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1367331.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19006..19329)
                     /locus_tag="OQL90_000443"
     CDS             complement(19006..19329)
                     /locus_tag="OQL90_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1367332.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19314..19721)
                     /locus_tag="OQL90_000444"
     CDS             complement(19314..19721)
                     /locus_tag="OQL90_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1367333.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            complement(19718..20281)
                     /locus_tag="OQL90_000445"
     CDS             complement(19718..20281)
                     /locus_tag="OQL90_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1367334.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(20272..20742)
                     /locus_tag="OQL90_000446"
     CDS             complement(20272..20742)
                     /locus_tag="OQL90_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1367335.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(20927..21721)
                     /gene="thyA"
                     /locus_tag="OQL90_000447"
     CDS             complement(20927..21721)
                     /gene="thyA"
                     /locus_tag="OQL90_000447"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1367336.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(21728..22603)
                     /gene="lgt"
                     /locus_tag="OQL90_000448"
     CDS             complement(21728..22603)
                     /gene="lgt"
                     /locus_tag="OQL90_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1367337.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(22754..25000)
                     /gene="ptsP"
                     /locus_tag="OQL90_000449"
     CDS             complement(22754..25000)
                     /gene="ptsP"
                     /locus_tag="OQL90_000449"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1367338.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVVAFMERRGM
                     GGLIRGGL"
     gene            complement(25013..25543)
                     /gene="rppH"
                     /locus_tag="OQL90_000450"
     CDS             complement(25013..25543)
                     /gene="rppH"
                     /locus_tag="OQL90_000450"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1367339.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            26228..26917
                     /gene="mutH"
                     /locus_tag="OQL90_000451"
     CDS             26228..26917
                     /gene="mutH"
                     /locus_tag="OQL90_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1367340.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            26986..27699
                     /locus_tag="OQL90_000452"
     CDS             26986..27699
                     /locus_tag="OQL90_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1367341.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            27837..28055
                     /gene="ygdR"
                     /locus_tag="OQL90_000453"
     CDS             27837..28055
                     /gene="ygdR"
                     /locus_tag="OQL90_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1367342.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            28163..29203
                     /locus_tag="OQL90_000454"
     CDS             28163..29203
                     /locus_tag="OQL90_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1367343.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLNLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGGMRYLHLADKHDLPSIVTIQTPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(29235..30428)
                     /gene="lplT"
                     /locus_tag="OQL90_000455"
     CDS             complement(29235..30428)
                     /gene="lplT"
                     /locus_tag="OQL90_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1367344.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(30421..32580)
                     /gene="aas"
                     /locus_tag="OQL90_000456"
     CDS             complement(30421..32580)
                     /gene="aas"
                     /locus_tag="OQL90_000456"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1367345.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            32969..34000
                     /gene="galR"
                     /locus_tag="OQL90_000457"
     CDS             32969..34000
                     /gene="galR"
                     /locus_tag="OQL90_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1367346.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(34007..35269)
                     /gene="lysA"
                     /locus_tag="OQL90_000458"
     CDS             complement(34007..35269)
                     /gene="lysA"
                     /locus_tag="OQL90_000458"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1367347.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            35391..36326
                     /gene="lysR"
                     /locus_tag="OQL90_000459"
     CDS             35391..36326
                     /gene="lysR"
                     /locus_tag="OQL90_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1367348.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(36313..37005)
                     /gene="ygeA"
                     /locus_tag="OQL90_000460"
     CDS             complement(36313..37005)
                     /gene="ygeA"
                     /locus_tag="OQL90_000460"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1367349.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(37134..38549)
                     /gene="araE"
                     /locus_tag="OQL90_000461"
                     /pseudo
     CDS             complement(37134..38549)
                     /gene="araE"
                     /locus_tag="OQL90_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(38864..39625)
                     /gene="kduD"
                     /locus_tag="OQL90_000462"
     CDS             complement(38864..39625)
                     /gene="kduD"
                     /locus_tag="OQL90_000462"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1367350.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(39655..40491)
                     /gene="kduI"
                     /locus_tag="OQL90_000463"
     CDS             complement(39655..40491)
                     /gene="kduI"
                     /locus_tag="OQL90_000463"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1367351.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(40778..41959)
                     /locus_tag="OQL90_000464"
     CDS             complement(40778..41959)
                     /locus_tag="OQL90_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1367352.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            42214..>42610
                     /locus_tag="OQL90_000465"
     CDS             42214..>42610
                     /locus_tag="OQL90_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1367353.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSL"
BASE COUNT        10842 a        11397 c        10106 g        10265 t
ORIGIN      
        1 taaattatcc gcctcacttt tgacggggat ttcaacagat aaatcacaac ttatcgcaag
       61 cgatgagtga tgactgcgga taaaagagat caaataatta cccatttttt tcaaactagg
      121 cagtgtcgcc aggatttccg ccgtctcacc actgtttgaa ataagaataa caacatcgtc
      181 tttcgccacc atcccaaggg cgccgtgtac cgcttccgta ccgtgaacaa aaaacgaggg
      241 agttccggtg ctggcaaatg ttgcagcaag ttttctggca ataatgccag atttcccaac
      301 accaataaaa acaacttttc cctggcaaga ttgtaatatt ttaattaaat tcgcgacttc
      361 cgaaaggggg gaatcaataa cctcttctac tgctttatgt tcgttacgca taaaacgttt
      421 aataagatgg ataagatccg tattgttcat tttctttttc ctgattaata tttttaatta
      481 cactcgcaag gcgcgcaagc cccatatata atttttctcg actgcaggcc agattaagac
      541 gcagatagcc ctctccttca gtaccataaa catgccccgc cataatacct actctccctt
      601 cgttaattaa tcgacggtaa aatttatcaa agggataacc aagggccgag caatcaaccc
      661 acgaaaaata gcagccctca gctggccata cgatcaattc cggaatattt tcatttaaat
      721 actgcaaggt aaattgaatg ttagaggcaa tatgtacctt taatgacttt aaccattctt
      781 cacattcatt ataggcaaca atggtcgcaa ttacattcag tactgatggg gagtttacga
      841 aatcgcgata cctcgatatc gttctgaact tatgattaaa attatcgtca gttgaaatca
      901 tatatgatcc ggttagcgca ggaacattaa atgttttcga aatcgaatta aacaccacca
      961 ctttgtcatc ttttattgtt agcgctgacg tgaaactatg attaaaaaca aaatcactat
     1021 gtatttcatc tgaacataac caaatattat gccttgcaca gcatgctgcg attctgtgaa
     1081 gatcgtcttc cgaccagacc gttcctgtcg ggttatgagg attacataat aacaccatct
     1141 tcgcgctttt aattttaagc tcaaagtctt gccagttgat aacgtagcct ctcccatttt
     1201 ctttcatcaa agaggttgtt agcatgtttc gaccattatg cttaatcaca tcaaaaaatg
     1261 cgttataaac aggcgtgaaa atgagaatat tatctccatt caatgtcaat atttctattg
     1321 ccttagatat tgaatacatc acgcttgggc tataggtgat ccattctggt tgaatctctg
     1381 ttttatattg ttgatgatac caattgacta tcgaattcag atagtcatca tgctgccaac
     1441 gactgtaccc taaaatcggg tgttccagac gtttttttaa tgttctgatg atagcttcct
     1501 gtatcggaag atccatatct gaaatagaga atggtaatac atcttttaca ccaaatctgt
     1561 cttcacaaaa gtcccattgt gtgcaatatg ttcctttacg agaaattgag gtattaaaaa
     1621 tatccatcaa catatcccca tgtaattatc catatcccga cgtagggcga tgacttttgt
     1681 accgataatg acttgtaagg tattttgatc aagtttcaca accgcaattc ctccggccct
     1741 ttttattgca gcctcattta tcaatcccat atccgcgata gttaaacgca aacgagttac
     1801 acaattatcc agtgatttaa tattttcttt accacccaga gccttaacca tttgctccgc
     1861 tgcctgcaac tctgatttat catgaactgg gatgttagct attgcagttt cttcgtgagc
     1921 gttcagcgtg acaggactgg actcttcacg gcctacagtc atcaggttaa atttgagaat
     1981 ggtccaacgg aacacaaagt aataaatgaa gaaataagcc acttcaacac ataccgctaa
     2041 tggccatccc gttcttgtgc cacgtaagac accaaagcta ataaattgaa tcagatcgcc
     2101 agtaaagccc atcttcacgc ccatatatgg cagaaccatg ttagccaatc cattcataaa
     2161 ggcatggaag acaaacaaca gcgggctgac aaataagaac agaaattcaa tcggctcgct
     2221 gacgccgcca ataattaccg tgagcacacc ggaaatcagc aatgctttga tagcccttct
     2281 gttttgagca tgagcagtgc gatacatcgc cagcgcggct ccagccagac catactgaat
     2341 catcagtttg ccctgctcca ttttccccgc caaatccagc ggaatgaccg cattctcttt
     2401 gacgtatgcc ataaacatgt tcagggtgcc gtaatactct tcaccgccaa tcactgccga
     2461 tccgccgatt ggggtaaagc ggaaaactga tgtcaccaag tgatttaagc caaaaggtat
     2521 cgtcacgcgt tcggcaacag catagaagaa ataaccaaca ggaccggaag ttgaaatcca
     2581 gtgaccaatg agcatgaaaa gattgaaaaa tggcggccag ataaaaggaa tgatcagacc
     2641 aaatccactc atcacaatca gtgtaatgat aggcacaagg cgtgggccac tataaaaccc
     2701 taattattca ggaatacgta agttaacgat ctttttatac atgctggcga ctaataaccc
     2761 tgccacaatc ccacccaaca cgctggtatt gtaggactgg atcccgagta tgatggtttg
     2821 cccatgtgtc gacatttggt cagcagctac caataagtcg tgctgtttaa gataaaagtt
     2881 cgttccaaga tgcatcgcca taaagccaat taagccagaa aaagcgccat aggctttatc
     2941 atctttctct tttaataatc ccaaaggtat agctatcgca aacaatacag gtaaattaac
     3001 gaaggcaaac aaaccaaggc taacaataaa atcaagtatg gttttaatta acggaatagc
     3061 caacactgga atgaattttg ccatatcatc gctggctaaa ccacttccca gccctaacat
     3121 cataccacat acacttaaca gagcgatggg atacataaat gccttcccca ggctctgaaa
     3181 aaaactccag gatttctttt gtttcatact ggttatctca tgaaaatgtt atgtataatt
     3241 agtccattaa tactttgatg cgaatagaaa gatatagttt ctcttccaga atcaaatcat
     3301 atttaaaagt tgcgtacaga tatctttcaa tttcatgaat ggcacgttgt aactgaaaat
     3361 ctttttcaag aaactgctca tagatatcac caatattttt ctgttttatt ttttcattac
     3421 ataacacacg actagaaaaa tatttaaggt gcatcataaa tctggagtag ttaaatgaac
     3481 ttctgtccac ccttctgtta taaacaactt caataagctc cgtgattttt tgagacagct
     3541 caataatcct tactgattca taatcaactg aattttcata attatttaaa atatgcaatg
     3601 ctataaaacc aatctcatca tccggtagtt gaacgctaaa ttgctcatta atgattgtta
     3661 atgcatcacg acttattgca tactctgctt tgtacaacgc tttgatttcc tcaaggaaaa
     3721 cattattaat ttgtatgcct gagacgactc gggatattgc attcgaaatg tgatcagcaa
     3781 gaatcagtag tagcgatgga ttgagttttt tttcgagatt actctccgca taatgcacta
     3841 tcttttcggc aacaaaaata tactcaatac ccacatgctc aatcaatttg tagagtttat
     3901 ttttttgctc atttctgaca taaaaaatcc ggtttgcagg atgctcaggg accgacatac
     3961 catattttta ttgtaaccaa cgccaggccc agaaatgata acctcctgtc cgttcaccga
     4021 tgcctgtaca caattattgt tcatgacttt ttcgatgatc attccatggc tccccaaaaa
     4081 aaaggcaagg catgtcagaa gtaataatta cttcaaacat tgccttgcct gatttaacag
     4141 ttgcaagata attaactaat gcgtatacct tacacggaat tgtgcttaat aaaagagatg
     4201 acagacttat tttttaagcc gatattcaaa tattcgacag cagtcacaaa accatcgttc
     4261 aacatttatg taaataatat ttattcaatg aaacataaat tcattgaata aattaaaaac
     4321 atcatcccct tctcgccagc gtcgccccat cggcaaaata cgctttaatc cccgcaagaa
     4381 tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct tcctcaacgt
     4441 tactaataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt agtacggcaa
     4501 acccggcctg ttcaacttga tttttatgca gcttgttgat tttaccgagc ttattcagta
     4561 ccgctttacc aaacttcagg ctgtcggcaa ttgtcagcga ctgcaccata tcgaacatgg
     4621 tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct
     4681 gggtttgtgc cagatatttt gccgcagtac tggttgcacc tttggttgag agcgcaaaca
     4741 cagaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag acaaacaggt
     4801 cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag atgtcttcat
     4861 tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc agacggcgag
     4921 ctatttgcaa taccacgtct ttttcgcgcg ttttgtattt ccccaccgca ccggagtctt
     4981 cgccgccgtg gccagggtca agcataataa caataggacg atcgcgccct gctttacccg
     5041 gttgtggacc actttgtgct ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct
     5101 ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag aggtccatca
     5161 ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt tttacgtttt
     5221 gctttaattc aaaaaccatc cgtacggtct gcgggtcaaa ttgcccgacg cgcgccgact
     5281 tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc gagttcaggt
     5341 ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac tgcttatatt
     5401 tcagctgacg atttgattct accgtcacac gggtgtagct ggacgcaggc cagacgcgca
     5461 ccgccacgac ctggctgacc gcagccagac tgacctgact tacgctcaat agccacatgg
     5521 cacccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct gacatgcctc
     5581 tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga ccggaaactt
     5641 tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt atattcacgg
     5701 cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca
     5761 gaataaaaat acactaattt agaataatca tgcaaagggg tgtgccgtgg taaaggaacg
     5821 taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata cccaccgggg
     5881 aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt tctccagtat
     5941 cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg tctatggcgc
     6001 acgtccgcag atcgacgcaa atctggccgc gcatcaccac gaaccgctgt atcacaagaa
     6061 tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg gaacattgca
     6121 actggacatc actgctcgcc tgtcgatgag tctcaataac acgccgctac agggcgcgca
     6181 tatcaacgtc gtcagtggca attttattat tgcccagccg ctgggcgtcg atgacggcgt
     6241 ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc atcgtcaact
     6301 ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg gcgagagctt
     6361 taatctgacc tcggaagaga ttgccactca actggccatc aaactgaaag ctgagaagat
     6421 gattggtttt tgctcttcac agggcgtcac taatgacgac ggtgatattg tctcagaact
     6481 tttccctaac gaagcgcaag cgcgggtaga agcccaggaa gagaaaggcg attacaactc
     6541 cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg
     6601 tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct cacgcgacgg
     6661 tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga
     6721 tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc tggtgcgccg
     6781 ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc gcgataacac
     6841 gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg aaatggcctg
     6901 tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc tggaacgcat
     6961 tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca cgcgcagtat
     7021 tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc ccgagagcaa
     7081 aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt tagggtaagc
     7141 gatgaaaatt gtcggatgcg acatgcgtaa cactcgtgcg tcgcatccgg caattacgtt
     7201 tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg agtggcgatt
     7261 gccgcactta atatacgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcacc
     7321 gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc
     7381 gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc
     7441 tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc aaaccagacg
     7501 cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg
     7561 ctgtcattac gggcaatcat caccggtcga ccttcgtacc aacgagagtg cggatgacga
     7621 tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca
     7681 aacggccctt cccgcagggc gcacaaaagt tggtactcat tgaacgcctg aatgattaaa
     7741 tccggctcgg cacgcgcttg cagcagatcc agataacgtc cataacccgc aagagcttcc
     7801 tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat atcagtaaaa
     7861 tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc cgcagctaac
     7921 tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca gagactgtcg
     7981 cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt caggcggctt
     8041 agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat atcgcccagc
     8101 acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca
     8161 tgatcgggca aggcgtcgat cagtctcgac atcataggca gatcgatcat tgacgcttca
     8221 tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta
     8281 cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt
     8341 tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg
     8401 gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc ggccatttga
     8461 attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg gccgccggaa
     8521 atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca gttaatttca
     8581 tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg
     8641 gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca catgcgattc
     8701 aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct gctgaccgct
     8761 tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc
     8821 gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt
     8881 ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg atgttcatct
     8941 cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg ctccacagct
     9001 tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct
     9061 catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat
     9121 gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga cgatcatagt
     9181 cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa agctgatatt
     9241 gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg taagccgaac
     9301 tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga
     9361 atactaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc agcggcgggc
     9421 agccagcgga tagcggatca aactggcgaa ttagtgcatc aagctggcta gcgatgagcg
     9481 gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg gaaagctgac
     9541 tcaggctaac ccccttttca ttgagaggtg cctggaggac agccgtgatc cactcggtca
     9601 acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt acccagttcg
     9661 ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg
     9721 acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc
     9781 ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga ccacgctgct
     9841 gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt agtgtcctcg
     9901 cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca ccagtttgtg
     9961 ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg
    10021 catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt
    10081 cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa
    10141 ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcgacctccg
    10201 cgaggtcgac gctttttggc gcagcattaa gatccaaaac tgcctcaaac gagtggcgat
    10261 cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac cagaccagcg
    10321 gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt ttatcacttt
    10381 cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc
    10441 agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc agttcgctga
    10501 tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa
    10561 tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt
    10621 gccgataacc atcgaactct tcaactaccg catcccacgc atgttcgtca ttattcagcg
    10681 tttcgatatc cagcgcgttc agccccatca ttgacgttgc cagcgcacta cgcagggtgt
    10741 tctcacgttc gggcgtcatc accgcctgca ataaccaaag catttcctgc gcttccagag
    10801 tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc aacgttaagg
    10861 catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac
    10921 gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta
    10981 gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag tcgccaacgc
    11041 cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca cctttaaata
    11101 caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt atttcgcgaa
    11161 acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc attccaggtg
    11221 cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc gccttcatat
    11281 aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg ccaatcagca
    11341 acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt
    11401 cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca
    11461 acacctcacc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac
    11521 ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc
    11581 gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg atcgcctcaa
    11641 acagtggatg tcgcggggtt tcccccccag ctttcgtgcg atcttctaag aaacgctggg
    11701 agaatttttc cagcgactcc ggcaactgat aactgttggt ctcttcttct gcccaggcgc
    11761 tgatcttgtc gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag
    11821 aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttaccgtag
    11881 caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg
    11941 ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg
    12001 gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg tagcagtggc
    12061 gacgccagaa atcggcgcag gcctggtaac gtagcagaga ctcatcttca atcagctgct
    12121 gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg caaaaaccgt
    12181 gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg
    12241 cttgcgcttt atcgtcgatc tcttccagca gacgttcgta cagtggattg tcggtagttt
    12301 cacgcagaca ggcgatgcgc aactcatgga tattgctacg gatacgacca cgcaattctg
    12361 ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc gggcggggaa
    12421 aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg
    12481 ttttgcctgt gccggcagag gcttcaatca ggcgctcacc ctgtaagggc aagcgcaaag
    12541 gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc
    12601 aacgcgctga cgttctccca cactttccag ccttcagggt gtacatattc ggctttcccg
    12661 ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac caccgcctga
    12721 tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca
    12781 cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg
    12841 ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat ttgcgcaaac
    12901 tcatctggct tcatcgctcg caattttgcc tctgtggttg ggaaaaacgc cttgtaacgc
    12961 tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga
    13021 cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc ggtacgcaac
    13081 tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc tgagctggtg
    13141 tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttaccg
    13201 gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt tcgacaccac
    13261 tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg cctgcgttgt tgcctgggcc
    13321 tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga
    13381 taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc atctcgcgag
    13441 aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa cgctttaccc
    13501 ttttctgcgg aatccatcat ctggttatac caagacttcg cctgctcaag ctgatcttcc
    13561 gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg cagacgctgg
    13621 gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa acttatgcca
    13681 ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc
    13741 gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg
    13801 acatcagctt tgggttcgct ggaaaaataa cggcttggcg catacaccac gcgcagattc
    13861 gactcatcaa caatcagctc tggatggtca tatttcttct ctgacttaat cagcgagaaa
    13921 tcgtcaggaa tataggggtt aagctccggt aaggagagcg caatattggc agctttttgc
    13981 tgccagtcag cgaaagtttg ttcgctgatt ttatcgacct gatacggcgc atcgacaaaa
    14041 taagccgttt tattgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc
    14101 gtcatcatcg ccagacgttc ttttactgct ttagcatcgt accgatcggc aatattgact
    14161 gcatccagcg tatgctcaac cggaacgcga atcatggtat ctgccagcca ttcgacgtaa
    14221 tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt cgccagttca
    14281 tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct aaaaattgcc
    14341 gccacaacct gatcgcgatt agccaggcct ttatcggtta aagacgcaga gatcgctaat
    14401 acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc aactaatccc
    14461 tgcttttgca gccagtcgga aagtgtacct gggctgcgat taccaatcag ataggtaatc
    14521 aattcatccg ttttactacg gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc
    14581 agaactttac gcggcagtgc cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg
    14641 actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg
    14701 tccgccgcca tttttgccaa ctccggcagc ggtttattgc tgtaaataac cgccttcatc
    14761 agattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac cggattacca
    14821 ggtttatcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg
    14881 ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt
    14941 aattcagcgt tcaccgcatt acgctcacgt tcggcatatt tcttgtcgag caaaggttca
    15001 gcaatagcat cggccaggcg gtctaccgca ccaggcaagg cgtcgttctc aacttccaga
    15061 tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg cattttgaga
    15121 tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca
    15181 aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcaccacc
    15241 agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac cataccgtta
    15301 tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga
    15361 atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac taacaacaat
    15421 aatgctttga accaagtgct gcggggcatt cacggacctc ataatcaact taattttctg
    15481 tccagattca acacgttaac gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt
    15541 cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag taagcggata
    15601 gattgcgcaa tttttataca gcactcatga ctgattaaag cgaaacagcg gtaacaggaa
    15661 acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc gccagagcct
    15721 ttgataccag atattatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag
    15781 gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc
    15841 gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga
    15901 catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc
    15961 aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt caccattacc
    16021 accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa
    16081 taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc agccagttat
    16141 ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc gacaggcaat
    16201 gactctgtcg gcaagctgct gcatctcctg gcactgagtt tcccagaaaa tttcaccaaa
    16261 agcgccatac ggtaaatccc ctgccgcccg gaagcgacgg aacaagcgtt cggcatcatc
    16321 ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa gcccttccag
    16381 aataaatggc tcggtgtcgg ggatttcgct gtcttcggta cggaagttca cctgcaaacg
    16441 catctggaaa aatgcccgca ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac
    16501 ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt taccagcctg
    16561 gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg
    16621 tggatcaaac ggcatccggg tatggaggca ggtaagatgc gcttttaccc ttgcctcgct
    16681 ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga tgtagtcgat
    16741 cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg aaagcccgat
    16801 ataactgata tagagttttt gctgcgcgga aattaacgct tccaggaaca ggtagcggtc
    16861 gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt caaagcccaa
    16921 tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa
    16981 cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg
    17041 gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg acagcggcac
    17101 cgcgtcgcca tactgcgccc ctaaaccttc ggcgataatc gcctgccatt gttgttcgat
    17161 cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg
    17221 acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt
    17281 tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgactc
    17341 atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa
    17401 catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg
    17461 aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccaca ggcgtaaata
    17521 acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagtaatgc
    17581 cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga taaacgcttc
    17641 cagtaccgga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc
    17701 cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga tatcagccac
    17761 catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg ccagcaggcg
    17821 atcgtgcaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg tgatactgct
    17881 atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc
    17941 agcaacggca cggttttcca gttccagaat gtcagactga atgttatgca gcaggttatc
    18001 tggcgtgaca tcgacaaagg cgtccagctc ctggctgctc tccaggtcag aaaggagata
    18061 aatgtagtcg cgcccaagct tcccccatga ggccagcagc gggttgccga catcctgttc
    18121 accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg gcaattcgcg
    18181 atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag caggatcttt
    18241 aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt
    18301 acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa
    18361 gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg
    18421 ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat attccaccag
    18481 cgccttccac aacggcgctt gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc
    18541 tgtttcccat tgtgccagcc actccgggcg atagaccaga tactggtcaa acaggtccgc
    18601 cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca gataatgccg
    18661 caacagggta aagtcttcgt gctccaacag ttgcggcagc agagtcatca gtttccagct
    18721 catgctctgt ttgttaaagg cgctctcttt ggggatctcc ggtaacaccc ggacgaacat
    18781 atcccagata aagctcgcag gcagcggaaa atcaatgttt gccgcaatac caaacttttg
    18841 cgacagggtc atttgcagcc actgtgccat accggtactt tgcaccagaa tcatctctgg
    18901 ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac
    18961 gtccagacga ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg
    19021 gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat
    19081 gctgacacat cccgcctgcg atgtctgcaa tcggttgacc tgccagttag ctggtggcga
    19141 aatcgcgcgc agttgcgttt gctgccagcc atgccgccag agctgttggt actggtttct
    19201 gctggcaaga ctgttcatca atgttcgctg gaaacccgat aacgcagtga caatcatcac
    19261 catcaacacc atcgccaaca ttacctccgg caggctaaag ccttgttgat tcttcaggga
    19321 agctgacata acgccctctc tttcagcgga caaaaatcgc tccagccgct tggcgaaaaa
    19381 acaatgtttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc ataaccggca
    19441 atcaataagg cttcattatc tgccagtaaa cgcaaacaaa cctgggcatc ggtttcagcg
    19501 tactgcgagc actgaactgc tggctgcgtc tgccaggagt gcatttttcc ccacgccagc
    19561 gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac gcgagaggca
    19621 aaactgcgat cctgttggct cattccttgt aatagcaaac tacccaaaac cagcagcatc
    19681 aggaccagag ccagtgacga aacacccttt tcgcggttca caggttgaat cctgtcacgc
    19741 tatagctggc attcaccacg gtttgcggtt cagacttact ggcagcacgc atattaaccg
    19801 tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga
    19861 tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta
    19921 gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct tttaccggtt
    19981 cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag tcaccctgtc
    20041 cgacaatttc cagcccttcg ccggtacaga tgccatgaca ataacccgcc ctctggagat
    20101 gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc ttacgggtgc
    20161 tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc aacaatacgc
    20221 tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct tttacaggca
    20281 tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa ccaccagcca
    20341 ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc
    20401 aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc gtggcacaaa
    20461 gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca caaggcacca
    20521 taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt tggcatcttc
    20581 acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccatagcc gttgcgactg
    20641 ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa tcagcatcgc
    20701 gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag tatggagcga
    20761 ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg actatttacg
    20821 ccgttgcagg acgttgcaaa atttcgggaa ggtgtctcga agaatttaat ggagggtaaa
    20881 aaaaaccgac gcacactagc gtcggctctg gcaggatgtt tcgtaattag atagccaccg
    20941 gcgctttaat gcccggatgc ggagcgtagc cttcaatctc aaaatcttcg aaacggtagt
    21001 cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc
    21061 ttaattgcag atgagtttga tccatatgat tgctgtacag gtgcgtgtcg ccccccgtcc
    21121 agacaaaatc gcccacttcc agatcacact gctgcgccat catatgcacc agtaacgcgt
    21181 agctggcaat gttaaacggc aggccgagga aaacgtcaca ggagcgctga taaagctggc
    21241 aagagagttt gccgtctgcc acatagaact ggaagaacgc atggcacggt gccagcgcca
    21301 ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt
    21361 ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatcc ggcgttggcc
    21421 aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc gttttcatcg gcccattcgt
    21481 cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcagaaacc
    21541 acagcagttc atggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc
    21601 catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac
    21661 ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca
    21721 tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca
    21781 cacccgcgac aatcatcggg atggaaagaa tttgtcccat gctgatgtac tgcacccagg
    21841 caccagtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt
    21901 aaccaatcag gaacaaacct gagacagctc ccattgggcg cggtttacga atatacaggt
    21961 tgaggataat aaacagcact acaccttcca gcaacagctc gtaaagctgt gatgggtggc
    22021 gcggcagcac accgtaagta tcgaaaatgg attgccactg cgggttggtt tgcagcagca
    22081 aaatatcttc tgtacgggag ccagggaaca gcatggcaaa cgggaagttc gggtcaacgc
    22141 ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa
    22201 tgagtggtgc gataaaatca gagacctgga agaaggaacg tttagtacgg cgggcgaaga
    22261 taatcatcac cacgataacg ccaatcaggc cgccgtggaa ggacatgccg ccgtcccaga
    22321 cacggaacag atacagcgga tcggccataa actgcgggaa gttgtagaac agaacataac
    22381 caatacgtcc accgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat
    22441 ttttggtcca gccactgccc ggacgattcg cccgacgtgt tgccagccac attgcaaaaa
    22501 tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa
    22561 tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct
    22621 tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca
    22681 aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg
    22741 tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga cgctccataa
    22801 aggctacaac ctgatgacga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa
    22861 gattttctgc ttcggcaaaa tcaatgcgcc gcagcaggta ttttacccgc gctacagaac
    22921 gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat
    22981 cgcccgccat ttcaccgcac aaacggagat cgataccatg tatttccgct tcccgggcga
    23041 tcattgccag agctcgtaac attgcaggat gaaggctgtc ataaatgttc gccacccggg
    23101 tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca acagagatga
    23161 aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggtact tccagcatga
    23221 tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac
    23281 gttcaatcag gcggcgtgct tcgtcaactt catcgaggct ggtgaccatc ggcaacagaa
    23341 tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga
    23401 tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc
    23461 tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg
    23521 gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg
    23581 ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg ccatcaatac
    23641 ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg acttttatac
    23701 gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga cggctaagct
    23761 caatctcttc actaattagc cgctgatatt cttgcagcag tatcggctcc ggatcgacca
    23821 gcaattcacc gcgatagcca tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa
    23881 tatccgcgcc catcacggta gggagcccca gcgcacgtac catgatcgca gcatgggagt
    23941 tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc tcagcaagag
    24001 ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt
    24061 gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcgccagccc
    24121 gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg
    24181 tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca
    24241 gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg
    24301 ctccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc
    24361 cggtcaatcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca
    24421 ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta
    24481 atgcgcggat tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc gactgagaaa
    24541 gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact
    24601 ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg
    24661 gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg
    24721 ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga
    24781 tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg
    24841 ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag acctcggtat
    24901 ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc
    24961 tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc
    25021 ttttacgtcg ataagcagat gtgttttgtg gttttggcgt attttcctgc agtgacatca
    25081 ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct
    25141 gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg
    25201 tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt
    25261 ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca
    25321 accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa
    25381 acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa
    25441 attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa
    25501 tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata
    25561 aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctgcag
    25621 ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt ataaacagag
    25681 gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg
    25741 gaattactac gggaaaaccc ggagcattct gaataagccc gatataggca ttttaaaata
    25801 ttcattataa gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta attcgtgacc
    25861 caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta tttatccaca
    25921 gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag
    25981 gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc tgtggataac
    26041 atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacctatt gcagtgctgc
    26101 gcaactggat tatcggcagc ctaaaaagca atataaatca gagaattgaa caacacatgt
    26161 ggaaaaagtt acactgcgaa tattcggtac ataattgctg tttgtttttt aaccaaggta
    26221 tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag aacagttgtt
    26281 agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac ttgccgggct
    26341 ggttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac tggagatctg
    26401 gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gctgctctgg gcgtggaact
    26461 taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg tttgtgttgc
    26521 cccgttaacg ggtaatagcg gggtgacctg ggaaaccagc cacgtgcgcc acaagctcaa
    26581 acgcgtactg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt
    26641 aggatccccg ttgctgtgga ccccgaatga agaggaagac cgacagctac gcgaagactg
    26701 ggaagaatta atggatatga ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg
    26761 ggaatattta cagataagac cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg
    26821 tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga agaatttcac
    26881 cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt
    26941 ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg
    27001 ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga gatcgttctt
    27061 gggatcgaca atattatttt cctttctctg gtggtggcaa agcttcccac agcacaacgt
    27121 gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc gctgctggca
    27181 tcaatcgcct gggttacgcg cctgacaaat ccgcttttta caatattcag tcaggaaatt
    27241 tctgcccgtg atttgattct gcttctgggt ggcttgttcc ttatctggaa agccagcaag
    27301 gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc
    27361 ctcggcgcta tcgtgcagat tatgctgctg gatattatct ttagcctcga ctcggtgatt
    27421 acggctgtgg gtctgtcaga tcacctgttt attatgatgg cagccgtggt gattgccgta
    27481 ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca tccttcggta
    27541 aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct ggaaagtttc
    27601 gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat tgcggttgaa
    27661 agcctcaact tgattcgcaa caaaaagaat ccgctctgat actccgtacg ctctcctgcg
    27721 ggagggcgta ctcactcctt ttaccctcac agtttaagat tttcctgctt tcaagatata
    27781 taacgtcgtt ttataaacag actattatca taggtaggcc aaatgaggat aaccggatga
    27841 aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca
    27901 gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa
    27961 ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta
    28021 accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca tccggctggc
    28081 cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg
    28141 tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt
    28201 cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca
    28261 cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta
    28321 cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg gcaactggct
    28381 ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg
    28441 caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata tccgcgaagc
    28501 gctgcatgac agcctcaagc gcctacagac tgattacctc aatctttatc aggtgcactg
    28561 gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg attctgcgcc
    28621 tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg cgggaaaaat
    28681 tcgttatatc ggcgtgtcga acgaaactgc atttggcgga atgcgctacc tgcatctggc
    28741 ggacaaacac gatctgccga gtattgtcac cattcagacc ccttacagtc tgttaaaccg
    28801 cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta
    28861 ttcatgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa aaaccgctgg
    28921 cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc
    28981 cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gaccctgctc agatggcgct
    29041 cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga
    29101 tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg tattagctga
    29161 aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac gccggttaaa
    29221 accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca
    29281 aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat taccgccagc
    29341 gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc
    29401 gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg
    29461 acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg cagcagctcg
    29521 tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg catacagcgt
    29581 gacacggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac gataccaatc
    29641 gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag cgccaccggt
    29701 acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc
    29761 accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt caggaaactg
    29821 cgggtcatgt tgatgagatt ccaagactgc cccggacgcg ccgccgccag tttgggaatg
    29881 tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag ggcgacgagg
    29941 acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc cgctatggta
    30001 gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa ttcgccgaga
    30061 ataccgtatt tcgccggtga ataggctgca gcaccaaccc ccaccagcgt atagccgaga
    30121 aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac
    30181 atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga
    30241 atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc
    30301 gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc agagagaaac
    30361 tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac
    30421 tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt
    30481 gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc
    30541 cggcacgccg tgctcgcggg catactgttg caacttatcg agcgtcagtt cgttatctgt
    30601 ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac
    30661 tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc
    30721 tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa
    30781 acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc
    30841 ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg
    30901 ccctttcagt tgcaggcgtc cgccctcttc gataccagga accgacaaca ggcgcgcatc
    30961 cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga
    31021 aacgacaggc gcgcactcgg ttacaccata gccttcaagg atgcgcaggc caaatttatc
    31081 ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca catagcgtag
    31141 acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc
    31201 gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatgcggt aatgcagcgg
    31261 gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag
    31321 cccaaaggag tgaaacagcg gtaatgccga cataaagcga tcgttggtgg tgaagtcggc
    31381 aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga cgacgccttt
    31441 tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg gctgctgttt
    31501 tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt cggcagtggt
    31561 gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag
    31621 atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt tgatttcagc
    31681 cgccgtaata gcactggtca gcccttttac cccagcagtg tagttcatca ttgcgggaat
    31741 gcggcgacgc gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat
    31801 taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt
    31861 tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga cacatttctt
    31921 cccggctccg aagcggtaca ttgcactcag taaagattcg tacagagttt cacgcgggcg
    31981 caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg
    32041 gtcacgggca cgcgacgcat ccggcatcgc cacctgcgtt ggaggcaaaa tatgcagagt
    32101 aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc
    32161 cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaaacccgc
    32221 gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac
    32281 cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag gttgcgtcgg
    32341 gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat
    32401 tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca aaatgccatc
    32461 aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg cctgggtgtc
    32521 acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat
    32581 tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag
    32641 aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc
    32701 aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatcac
    32761 cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag
    32821 tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg gctcacattc
    32881 ccacgatgaa aacacgccac tccttgaacc aacgggcgtt ttccgtaaca ctgaaagaat
    32941 gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag cccgactggc
    33001 aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag ccagcgaagc
    33061 ttcccggctg gctgtgcata gtgcaatgga gtctcttagc tatcacccga acgccaacgc
    33121 ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg atcgttggtg atgtttccga
    33181 tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt
    33241 cttattgatt ggcaacggtt accacaacga acaaaaagag cgtcaggcta ttgagcaact
    33301 gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa atgatcccgg atgccgattt
    33361 agcctcatta atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt
    33421 tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc tggctggcaa cgcgtcattt
    33481 aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta tttctgacgc
    33541 cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattg cggccaatga
    33601 ccggctggtg acatttggcg aaccagacga aagcggcggc gaacaggcaa tgaccgagct
    33661 tttgggacga ggaagaaatt tcactgcggt agcctgttat aacgattcaa tggcggcggg
    33721 tgcgatgggc gttctcaatg ataatggtat tgatgtaccg ggtgagattt cgttaattgg
    33781 ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc
    33841 aatcgtgacg atggcgaccc aggctgccga actggctttg gcgctggcgg ataatcgccc
    33901 tctcccggaa atcactaatg tctttagtcc gacgctggta cgtcgtcatt cagtgtcaac
    33961 tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa agcaattcca
    34021 gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa
    34081 acagaacttc tggtaacagc gaacggctat tgtagttgga tgacattgat gcgccatatg
    34141 cccctgtatc atgcagtacc agataatcac ctgccttcac ttccggcaag gcgcgggttt
    34201 caacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataac ggtccggcga
    34261 cgacggtttc caccgttggc gcgtgttcca gagaacgacc atcagctgcc agggcactga
    34321 tatggtggta actaccgtac attgccgggc gcatcagatc gttgaacccg gcatcaacca
    34381 gcacaaagtg gcggctcccc atttgtttga cgctccgcac ctgagtaatt aatacgccag
    34441 actgcgctac caggaagcga cccggttcaa tttccagttt cacagggtgg cccaaatggc
    34501 gggcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta tcaaccgcct
    34561 cttcaccctg ttgataagga acagaaagcc caccgcccgc agaaatagcc tgtaaatcct
    34621 gaccgaattc gatgacctga cgcaccatag caccacacac ctgttccaga tgggcataat
    34681 caacgccaga accaatgtgc atgtgaatgc cgaccagctg cagatgatga cgttgtatca
    34741 cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt tcgccaccgg
    34801 tattggtttt ttggctatgt ccgtgaccaa accccggatt aacgcgcagc catacccgat
    34861 gccctggcga aacctggccc agttggtcga gcatatcaac agaacccgca ttcaccggaa
    34921 tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa
    34981 tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa cgcacgctct atttcgccta
    35041 acgagacgga atccactttc acgccctgct cacgcattaa gcgcaaaata tgaatattgg
    35101 aacaggcttt ctgtgcaaag cgcaccacat caaactgttt cagcgctgca atctgccgac
    35161 gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttcagcgggc aaacgcagca
    35221 gattttcggc ggtgagatcg gtatcggtgc tgaacagtga atgtggcata acaaactcca
    35281 gataagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata tcgatttatg
    35341 tcgagtctat gcaaaaatga tatggattac cggattgcga gagagcgcta atggccgccg
    35401 ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc ctgactgagg
    35461 cggcacacct gctacacacc tcacagccaa ccgtcagccg cgaacttgcg cgctttgaga
    35521 aggtgatcgg gctgaaattg tttgagcgcg tacgtgggcg attacatcct accgtgcaag
    35581 gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcga attgtcagcg
    35641 ctgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc ctgccggtct
    35701 tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat cccgatgtca
    35761 gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc
    35821 atgatttagg actcactgag acactccata cgcctgcggg aacagaacgt accgaattac
    35881 tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc gctggcggta aaaaaggtat
    35941 taacgccgga tgattttcag ggtgagaact acatcagcct ttcccgtact gacagctatc
    36001 gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg atcgtagaaa
    36061 cccacagcgc cgcgtcaatc tgcgcaatgg tacgggcggg ggtaggtgtt tcggtggtta
    36121 acccgctcac cgcactggat tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg
    36181 cggttccgtt cacagtcagc ctgatccgcc ccctgcaccg cccgtcatca gcgctggtgc
    36241 aggcgtttag tgagcattta caagcggggt taccgaaact ggtcacttct cttgacgcta
    36301 ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga tcgccgcagt
    36361 atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc
    36421 aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg cataataagc
    36481 gcgtgacgct tcggtaaatt gccccagaca cagttcttca aaaataatct gattaatttt
    36541 cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg tcagccgccc
    36601 gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac gagtcattcc
    36661 ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca gagtgcaacg
    36721 tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa taccttctgc
    36781 gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt tatcccattc
    36841 cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca gcacttgcgc
    36901 agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcaaacgat agtaaggaat
    36961 ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc tctcctgatg
    37021 tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg ccaagtgccc
    37081 aatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg aaatcagatg
    37141 ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca
    37201 tttttggttt ccggaatgag ccagaaagtg atgcccacaa acgcaatgtt cagcgcagtg
    37261 tagagccaga acgtaccggc agcgccaatg ctgtcgagca gcgtcaggaa ggtcgccgat
    37321 aatcatattc gacacccagt tcgtggtggt cgaacaggta ataccgaaat cgcggcattt
    37381 cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca tcgcataacc
    37441 ggcaatacac atcatcgtca tgccaacaga gagccaggac aatccactgg aagccgtacc
    37501 gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca tcacgctgaa
    37561 accaattttc agagccggtt tacccctgct ttatctaccg taaacaccgc aataaaggtg
    37621 gcgaacataa aggtcagccc tacgaccaga gtcgcaatca tctgttgttc tgtggtcgta
    37681 aagcccgcca ttttgaagat acgcggcgcg tagtacatga tgatgttcat accggtaaac
    37741 tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc
    37801 ttaaacagtg cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc gttgagttct
    37861 tctcgcgctt tttccgacgt atcgcgcagc atacgcaata cttcttccgc ctcaatatga
    37921 cgcccctttt ccgccaacca gcgcgggcta tttggcagga agaccaccag aataatcagc
    37981 agaactgctg gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac
    38041 gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg
    38101 atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa
    38161 gacgcgatcc cgacagcaat gcccagcccc acacgagcgg cgattaacat ctctacgctg
    38221 gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg
    38281 tatttacgcc ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcgccg
    38341 agcatcatgc tactaaccac ccattcctgc aaatgactgg tcagcacaaa gtgatcggta
    38401 atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc
    38461 gcagcagcta ccgaaacaaa catattcata cgccgcgtat cccgcaaaga acgtggcgtt
    38521 aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacatagt
    38581 gaaaaaatac gtgaacaact cacgcaggtg tcaggtcgga aacagcataa atatggatta
    38641 aattgctgcg acatgtcgtt atgtgatgga tattccaatt ttcaaattaa gttgaattat
    38701 tgagattatt attaaccacc taattttaca gcagataaaa ttcataaagt tcattaattg
    38761 ataattaata tggattattt cataaccatg atatggatta tgatgatcta caggtataaa
    38821 aaaccctgcc atgcggcagg gtcataaaag taagaagaat gaattaacgc gccagccaac
    38881 cgccatccac ggcaatggta taaccattca cataatctga agcgctggag gcaaggaaca
    38941 ctatcggccc catcaggtca ctcggcagtc cccaacgacc agctggaatg cggtcgagaa
    39001 tttccgcgct acgttgttca tctgcccgta gttgttgagt attgttggtc gccatgtaac
    39061 ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgttcgcc atcaatcgcg
    39121 tcacacccat cacgccgctt tttgatgcgg tataagaagg cacacggatc ccgccctgga
    39181 aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg ataaagtgtt
    39241 tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg acatcgtccc
    39301 agtctttttc gctgaactcg agagcgtctt cgcggcgaat caatccggcg ttattcacca
    39361 ggatatcaat atgaccaaat tcagctaccg cgcgatccag cagtccagga atgccatcaa
    39421 tctttcgcag atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg acctgctcga
    39481 tggtttcagt cggttcaacg atgttaatgc caacaatgtc acagcccgct tgcgccagcc
    39541 ccaacgccat cccctggccc agcccagtat cacaaccagt gacgaccgca actttacctt
    39601 cgagagaaaa tgcacttaaa atcataacaa taccttattc gtttatgccc acaactagcg
    39661 caaatcttta acggccacat ggtccatatc atcaaagacc tggttttcac caaccatgcc
    39721 ccagataaag gtataagctt tggttccgac gccggaatgg atcgaccagc tcggggagat
    39781 caccgcctgc tcgttatgca tcacaatgtg acgcgtttct tgcggttgcc ccatcatgtg
    39841 gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc ggcgctcgtg
    39901 ggtgtgacac ggcatggtgt tccacaagtt acctggagcc agctccgtca gccccatact
    39961 caattggcag gtttccagca catccgggac aaaatatttg ttaatagtgc gacggttact
    40021 ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc ggggtgactt ttttggtggg
    40081 atacgtcgta tgcgcgggtg cgcaattgta ataaaacttt gccggagtgg cggtatcaat
    40141 actggcaaag acaacttctt ttgcaccttt accaacatac agggcgtcgc ggtgaccgat
    40201 ttcatagcat tggccatcga cagtaatcgt accggcaccg ccaatattga taacacctaa
    40261 ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta ccaacttccc cgccaacgga
    40321 aaccgtttta gttatcggca taatgccgcc aacaataatt cggtcaatgt ggctgtaaac
    40381 catggtgtac tcatcggcga caaatacctt ttcaaccaaa aattcattgt gcagcccttg
    40441 ggtatccagc gtttttgcgt gcgcactgtg gatgctctgt cttacgtcca catcaacctc
    40501 cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc gttttctcta
    40561 taaagggatg atatgcccat gggctagggt tttcaattaa aaataaaaca atgttttatt
    40621 tttaagttaa ggattaaaaa aagtgcagtg tcgatcacga atatgtcatg agaatggaaa
    40681 aatgagggag cccagaaaat gtatgtcgac ttgccagcca tcattattca acagattaat
    40741 gacggcagat atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt tcaatggtca
    40801 atgccacgcc ctggcccccg ccgatacaaa gcgttgccag ccctttgcgg gcattacgtt
    40861 tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct atcgggtgac
    40921 cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccactca agcatcttgc
    40981 caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg acatcagcca
    41041 actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca attcccatca
    41101 atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg ggtaaattca
    41161 acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg
    41221 atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa cgggctaaac
    41281 cttctgcact ggcgtcagtg catggctgtt catcggtatc aacaaccaac gtctgaccgt
    41341 tactttggat cattaccggg acgatctcat ctttaaatcg tccggcgtca atcgccgctc
    41401 gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg atgccatatt
    41461 cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca tcccacaacc
    41521 catcatgcac cagactgtca accaactggc tattgccaag ctgtgcaccg gtgcggctat
    41581 cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg acaatatcag
    41641 cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagccca gaaccgcaaa
    41701 cgtcattaat agtgattgca gaaacgctat taggaagacc acctttaata gccgattgcc
    41761 ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg
    41821 catatgcagg aacgccggta cgttctatta acgctttcac gaccaaacta ccaagttcca
    41881 cggcggaatg acccgctaac gcaccacgaa agcagccgat aggtgtccgt aacgccccga
    41941 caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg
    42001 ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaaa gaaatatgcc
    42061 tgagcagcag tcagagacat aactggcacg taaggtttgc aaccactaac ccaccaatag
    42121 aggggtagat agggcgttaa tctcccatac ttaacctggt ttatggtaaa ttgccctcca
    42181 ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc taaagaagaa
    42241 actctgtgga gtttcgcgct ctacggcaca gccgttggtg caggcacgct cttccttcct
    42301 attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt cgcctggcct
    42361 ttaacatatt ggccacataa agctttatgc cagttcatcc tctcatcgaa aacatcagca
    42421 ggtgaaggga taacgggcgc ggtaacacac tactatggca agaagattgg taatctgatt
    42481 accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt ggcaattacc
    42541 aactcactta cagaacagct ggcaaagcat atggttattg atcttcgcat ccgtatgttg
    42601 gtgagtctgg
//
LOCUS       DAJVOY010000009        42210 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVOY010000009 DAJVOY010000000
VERSION     DAJVOY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.009"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OQL90_000466"
                     /pseudo
     CDS             complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OQL90_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OQL90_000467"
     CDS             complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OQL90_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1367354.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYRVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OQL90_000468"
     CDS             complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OQL90_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1367355.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2414..3343)
                     /locus_tag="OQL90_000469"
     CDS             complement(2414..3343)
                     /locus_tag="OQL90_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1367356.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3389..4213)
                     /locus_tag="OQL90_000470"
     CDS             complement(3389..4213)
                     /locus_tag="OQL90_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1367357.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGLVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OQL90_000471"
                     /pseudo
     CDS             complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OQL90_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OQL90_000472"
     CDS             complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OQL90_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS1367358.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5081..5131
                     /gene="hisL"
                     /locus_tag="OQL90_000473"
     CDS             5081..5131
                     /gene="hisL"
                     /locus_tag="OQL90_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1367359.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    5107..5230
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            5277..6176
                     /gene="hisG"
                     /locus_tag="OQL90_000474"
     CDS             5277..6176
                     /gene="hisG"
                     /locus_tag="OQL90_000474"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1367360.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6182..7486
                     /gene="hisD"
                     /locus_tag="OQL90_000475"
     CDS             6182..7486
                     /gene="hisD"
                     /locus_tag="OQL90_000475"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1367361.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7483..8553
                     /gene="hisC"
                     /locus_tag="OQL90_000476"
     CDS             7483..8553
                     /gene="hisC"
                     /locus_tag="OQL90_000476"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1367362.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8553..9620
                     /gene="hisB"
                     /locus_tag="OQL90_000477"
     CDS             8553..9620
                     /gene="hisB"
                     /locus_tag="OQL90_000477"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1367363.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAIDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9620..10210
                     /gene="hisH"
                     /locus_tag="OQL90_000478"
     CDS             9620..10210
                     /gene="hisH"
                     /locus_tag="OQL90_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1367364.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10210..10947
                     /gene="hisA"
                     /locus_tag="OQL90_000479"
     CDS             10210..10947
                     /gene="hisA"
                     /locus_tag="OQL90_000479"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1367365.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10929..11705
                     /gene="hisF"
                     /locus_tag="OQL90_000480"
     CDS             10929..11705
                     /gene="hisF"
                     /locus_tag="OQL90_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1367366.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11699..12310
                     /locus_tag="OQL90_000481"
     CDS             11699..12310
                     /locus_tag="OQL90_000481"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1367367.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVAFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLLLVNPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="OQL90_000482"
     CDS             complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="OQL90_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1367368.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13531..13623)
                     /locus_tag="OQL90_000483"
                     /pseudo
     CDS             complement(13531..13623)
                     /locus_tag="OQL90_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13793..14797
                     /locus_tag="OQL90_000484"
     CDS             13793..14797
                     /locus_tag="OQL90_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HCS1367369.1"
                     /translation="MKFLVTGAAGFIGFYLSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="OQL90_000485"
     CDS             complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="OQL90_000485"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCS1367370.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKRQSP
                     LVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAKRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKNGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="OQL90_000486"
     CDS             complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="OQL90_000486"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1367371.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIT
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFHDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDNAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(17789..18622)
                     /locus_tag="OQL90_000487"
     CDS             complement(17789..18622)
                     /locus_tag="OQL90_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCS1367372.1"
                     /translation="MNHRLEKFSVLISIYKNDLPQFFEVALRSIFHDQTLKPDQIVIV
                     ADGELHQTHIDIINSFIDDVGNKIVTFVPLPRNVGLANALNEGLKACRNELVARMDAD
                     DISLPHRFEKQISFMINNSEIDVCGSFIDEIETVTEEFISTRKVPLEHREIVKFARKR
                     SAVSHPSVIFRKNTVLAVGGYPPFRKSQDFALWSLLIVHNARFANLPDILLKMRTGRN
                     LMARRGLSYLLYEYKVLYYQYKIGFIRKNELISNAMLRTFFRIMPSKLKELMYSIVRN
                     R"
     gene            complement(18619..19488)
                     /locus_tag="OQL90_000488"
     CDS             complement(18619..19488)
                     /locus_tag="OQL90_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1367373.1"
                     /translation="MDTVYAVLVAYNPEHNDLKNAVELLLRQVTKVVVCNNSTNGYKY
                     AENSSGDVKIFNFNDNLGIAEAQSIGMKWAFENGADFILQMDQDSIPDPKMVEQLLTC
                     YKKLLKQNVNVGLVGSQDFDKVTGELNKARVKKGKPLTEVYYEVDSTLSSGSLIPKNS
                     WLIVGGMKDELFIDAVDHEYCWRLRAAGFKVIRNKNALLAHRLGDGRFKILNILSVGL
                     PSPFRHYYATRNIFLLLNKNYVPIYWKISSLVKLIGKVFLYPIFLPNGNKRLYFFLKG
                     INDGLMGRSGKMK"
     gene            complement(19505..20692)
                     /gene="wzy"
                     /locus_tag="OQL90_000489"
     CDS             complement(19505..20692)
                     /gene="wzy"
                     /locus_tag="OQL90_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen polymerase"
                     /protein_id="HCS1367374.1"
                     /translation="MTSDFYNSKDKSLSVLLFFGFIFFLTRSFPFIQYSWIMEGFLCL
                     CIMSFTKKIANGIYHYPVILIFLLALFINFIYSYIKGNDIAIIIRFYIIILFILCAYF
                     CSYGTISIVKIFLYLMVLQAVIISIISIYMTKTYGIGDYSALRHYFLENDYGDVYTYG
                     SGFYRVQIKGNALIPFAFMLHIVIKDYFYYRFKNTITVILAIGTIVAGNFAYFVSICL
                     FFMYIILCSKSNSRYAKLRKIIFGVFLTVILPFFITYSIELIIMKSNGADSSLGVRWD
                     QFTVLINDLTESVSNFVIGSGLGNVIKIQTPIRDYSAYIYYELQSVYFLNQLGVILFT
                     LFLLINLLLTIKIIKYSELCVLYFLYVSYAITNPYILDSNHVAVIIVLVTLSNVLKKM
                     KAK"
     gene            complement(20748..21980)
                     /gene="wzx"
                     /locus_tag="OQL90_000490"
     CDS             complement(20748..21980)
                     /gene="wzx"
                     /locus_tag="OQL90_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen flippase"
                     /protein_id="HCS1367375.1"
                     /translation="MSIVKNTLWNISGYIIPSLIAIPALGILSRILGTEQFGLFTLAI
                     ALVGYASIFDAGLTRAVIREVSIYKNVHKELRAIISTSTVILTILGLIGGSVLFLSSN
                     VIVKLLNINANHVVESVKAIYIISATIPLYLLNQVWLGIFEGMEKFRKVNLIKSINNS
                     FVAGLPVIFCFFHGGLLSAIYGLVMARVLSLIVTFIFSRKLIISSGLSVKIVTVKRLI
                     GFGSWITVSNIISPIMTYMDRFILSHIVGADKVSFYTAPSEGIQRLTILPSALSRAIF
                     PRLSSELQSVKQTKILSYFIMVIGILPIVMLIIILSDFIMSAWMGPTYHGTPGIVLKI
                     LAIGFFFNCIAQIPFVSVQASGRSKITAIIHLLEVIPYLCILYIFIYHWGIVGAAIAW
                     SVRTSLDFLILLLIDTKY"
     gene            complement(21977..22534)
                     /gene="rfbC"
                     /locus_tag="OQL90_000491"
     CDS             complement(21977..22534)
                     /gene="rfbC"
                     /locus_tag="OQL90_000491"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCS1367376.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDSDRGIVWNDPILSIKWPTI
                     EHNNYILSIKDARAKELHNMKELFL"
     gene            complement(22539..23417)
                     /gene="rfbA"
                     /locus_tag="OQL90_000492"
     CDS             complement(22539..23417)
                     /gene="rfbA"
                     /locus_tag="OQL90_000492"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1367377.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(23475..24374)
                     /gene="rfbD"
                     /locus_tag="OQL90_000493"
     CDS             complement(23475..24374)
                     /gene="rfbD"
                     /locus_tag="OQL90_000493"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCS1367378.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKEANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAAKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELITTTAI"
     gene            complement(24374..25459)
                     /gene="rfbB"
                     /locus_tag="OQL90_000494"
     CDS             complement(24374..25459)
                     /gene="rfbB"
                     /locus_tag="OQL90_000494"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1367379.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTRFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTLEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            complement(25832..26725)
                     /gene="galF"
                     /locus_tag="OQL90_000495"
     CDS             complement(25832..26725)
                     /gene="galF"
                     /locus_tag="OQL90_000495"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCS1367380.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(26957..27952)
                     /locus_tag="OQL90_000496"
     CDS             complement(26957..27952)
                     /locus_tag="OQL90_000496"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HCS1367381.1"
                     /translation="MNNNVLLIGASGFVGTRLLETAIADFNIKNLDKRQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(28110..29504)
                     /gene="wcaM"
                     /locus_tag="OQL90_000497"
     CDS             complement(28110..29504)
                     /gene="wcaM"
                     /locus_tag="OQL90_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCS1367382.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMSNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLVYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(29515..30735)
                     /gene="wcaL"
                     /locus_tag="OQL90_000498"
     CDS             complement(29515..30735)
                     /gene="wcaL"
                     /locus_tag="OQL90_000498"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCS1367383.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQN
                     THAAWTKYNLAARTHWLQDEPTGKIAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLGTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(30732..32012)
                     /gene="wcaK"
                     /locus_tag="OQL90_000499"
     CDS             complement(30732..32012)
                     /gene="wcaK"
                     /locus_tag="OQL90_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCS1367384.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMMALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(32288..33766)
                     /gene="wzxC"
                     /locus_tag="OQL90_000500"
     CDS             complement(32288..33766)
                     /gene="wzxC"
                     /locus_tag="OQL90_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCS1367385.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCLAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPQGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKPLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQFLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(33768..35108)
                     /gene="wcaJ"
                     /locus_tag="OQL90_000501"
     CDS             complement(33768..35108)
                     /gene="wcaJ"
                     /locus_tag="OQL90_000501"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCS1367386.1"
                     /translation="MVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQML
                     GGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
                     IGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYH
                     DPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLL
                     IPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAEDIVLATLILLLISPVLC
                     CIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKV
                     GNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPCITG
                     WAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY"
     gene            complement(35213..36583)
                     /gene="cpsG"
                     /locus_tag="OQL90_000502"
                     /gene_synonym="manB"
     CDS             complement(35213..36583)
                     /gene="cpsG"
                     /locus_tag="OQL90_000502"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1367387.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTSVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSRDALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(36776..38212)
                     /gene="cpsB"
                     /locus_tag="OQL90_000503"
     CDS             complement(36776..38212)
                     /gene="cpsB"
                     /locus_tag="OQL90_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1367388.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSSVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(38215..39445)
                     /gene="wcaI"
                     /locus_tag="OQL90_000504"
                     /pseudo
     CDS             complement(38215..39445)
                     /gene="wcaI"
                     /locus_tag="OQL90_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(39442..39921)
                     /gene="gmm"
                     /locus_tag="OQL90_000505"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(39442..39921)
                     /gene="gmm"
                     /locus_tag="OQL90_000505"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1367389.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLPGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(39924..40889)
                     /gene="fcl"
                     /locus_tag="OQL90_000506"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(39924..40889)
                     /gene="fcl"
                     /locus_tag="OQL90_000506"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS1367390.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQSTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(40892..42013)
                     /gene="gmd"
                     /locus_tag="OQL90_000507"
     CDS             complement(40892..42013)
                     /gene="gmd"
                     /locus_tag="OQL90_000507"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1367391.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYCESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIVTGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLDDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(42039..>42210)
                     /locus_tag="OQL90_000508"
     CDS             complement(42039..>42210)
                     /locus_tag="OQL90_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1367392.1"
                     /translation="IGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPVNVVCRGN
                     PAVVIRERVETE"
BASE COUNT        11054 a        10726 c         9742 g        10688 t
ORIGIN      
        1 cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg
       61 gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg
      121 ttatcaccag caaaacggca accaacggcc acacagacaa attaaaagtc tcagcaatag
      181 agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca tgtggagaac
      241 gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct
      301 cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga
      361 agatataccc acctataaca gtaccaggcc acgggaacgc cccggcttcg taagtcagta
      421 ggcccgccta aattaacgca aaaaccccca cactttgtac cgaaattgca atcagcaagg
      481 cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa
      541 aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca
      601 ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc atgactggtg
      661 gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttcttaa tgaggatttc
      721 gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt gattattaga
      781 aaagatgatt atgctacgtc ttcgtactgc ggaaccgggt tacggaagct cttggtgacg
      841 caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct
      901 tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac
      961 tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg cgagatgacc
     1021 gacaggttaa cgaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt
     1081 accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc
     1141 ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac gccgtcacga
     1201 cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga cgccagtacg
     1261 gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg
     1321 atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc
     1381 aggaagtaag ttgcaaagat gaagatcatg ccgccgatca gcgcggtcag gaaaatcgca
     1441 cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat gccgtcaaag
     1501 ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg
     1561 ccagaccaga atggacgagt gctcgccagc gtaccagcgc cttcaccttc aaatacgcga
     1621 taaacaacca tgcccagaat caccgcgatc agcaccacct gcaacacgac gattacagtg
     1681 ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcatgaa ggccaccaac
     1741 gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata gattttcgcc
     1801 agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata
     1861 aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga
     1921 tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac
     1981 gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa agtatcaaac
     2041 agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt
     2101 ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt cattctcctt
     2161 tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac
     2221 aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa
     2281 tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt agtcattttt
     2341 tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca
     2401 taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga
     2461 tcgcactatt tgctaaaatt tcatcccgcc accagccact ggcaagtcct gcggtctgtt
     2521 cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc ttctcgatta
     2581 acgcgccact atcgagaaaa tgttgcgcca gataacgggg taaatagccg cagccgaggc
     2641 cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt
     2701 cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc
     2761 gatatcgctt aataatacga cgatttaacg gctcttcttc cagggctaac ggatgatgag
     2821 gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga aaaaccaaac tcactggaag
     2881 atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc agggcatccc
     2941 atgaaccacc cagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa
     3001 aagcttcaat cagtggcgct agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca
     3061 gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttcc cgcacggtat
     3121 gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt
     3181 gaccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga acggtgtaac
     3241 tcagagcgga cggcgtcttg tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt
     3301 ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt
     3361 gtatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat
     3421 actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc
     3481 cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg gccatctgcg
     3541 gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt
     3601 taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa
     3661 caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga tgacgccccg
     3721 gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc
     3781 agtcttcgag ttcttctaat actagtccac tgttggttac cggattacgc ggggtagttt
     3841 ctttcaccat gccttgcgca tcgccataga cagatgttga gctggtaaaa ataattcggg
     3901 gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat
     3961 cgccggagcc gctacgacgt gccggaagcg taatgaccag tgcatcggca tccatcaggg
     4021 catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa
     4081 tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc
     4141 agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac ccgacaattg
     4201 cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta
     4261 tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca
     4321 atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg
     4381 gactagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc ttcaaatggc
     4441 gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc
     4501 tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac tcaataatgt
     4561 ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt
     4621 tagcggggga acgcagtaga taagccgtct cttccagcga gttgtattct tcgagtgaca
     4681 tcagaacaca agcctctcca ttctgacgag taataaggat cggggcatga tcttcaacgg
     4741 ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt
     4801 caatctcctc ttttgtacag ttcattgtac aatgatgggc gttaattaac tatttattaa
     4861 ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc tatttttggt
     4921 gccgtcagct tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca
     4981 aatccatcgc catagaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg
     5041 ttaaaagaca tcagttgaat aaacattcac agagactttt atgacacgcg ttcaatttaa
     5101 acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
     5161 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
     5221 gcttttttat tgcgcggttg ataacggttc agacaggttt aaagaggaat aagaaaatga
     5281 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
     5341 gcgaattgct ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
     5401 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt cccggtctgg
     5461 taatggacgg cgtggttgac ctcgggatta tcggcgaaaa cgtgctggaa gaagagctgc
     5521 ttaaccgccg cgcccagggt gaagatccac gctactttac cctgcgtcgt ctggatttcg
     5581 gcggctgccg actttcactg gcaacgccgg ttgatgaagc ctgggatggc ccgctctcct
     5641 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     5701 aaggtatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg
     5761 gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     5821 tgcgcgaagt cgaagttatc tatcgctcga aagcctgcct gatacaacgc gatggcgaaa
     5881 tggaagaatc caaacagcaa ctgatcgaca aactgctgac ccgtattcag ggtgttatcc
     5941 aggcgcgtga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca
     6001 tcgccctgct gccaggtgcc gaacgcccaa ctattctacc gctggcgggt gatcaacagc
     6061 gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     6121 aagcgctggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     6181 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     6241 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcactgtta acgatattct
     6301 cgataacgtg aaagcacgcg gcgatgaggc cctgcgggaa tacagcgcga agtttgataa
     6361 aaccacggtt accgcactga aggtgtctgc tgaggaaatt gccgccgcta gcgaacgcct
     6421 gagcgacgag ctaaaacagg cgatggcggt ggcagtgaag aatattgaaa ccttccacac
     6481 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt gccagcaggt
     6541 cacgcgcccg gtcgcttcag ttgggttgta tattcctggc ggctccgccc cgctcttctc
     6601 aacggtatta atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     6661 ctcaccgccg ccgattgccg atgagatcct ttatgcggcg cagctgtgcg gtgtgcagga
     6721 cgtgtttaac gtcggcggcg cacaggccat tgccgcactg gcgtttggta cggaatctgt
     6781 gccgaaagtg gacaaaatct tcgggccggg caacgccttt gtcaccgaag cgaaacgtca
     6841 ggtgagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgtt
     6901 ggtcattgcc gacagtggcg caacgccgga tttcgtggct tctgatttgc tttctcaggc
     6961 tgaacacggc ccggactcac aggtgatttt actgacgccc gctgctgata tggcgcgtcg
     7021 cgttgccgag gctgtcgaac gccagctggc agaactgccg cgtgctgaaa ccgcccgtca
     7081 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tagagatctc
     7141 caaccagtac ggcccggagc acctgatcat ccagacccgc aacgcccgcg atctggtcga
     7201 tggcattacc agtgccggtt cggtatttct tggtgactgg tcactggaat cggcaggtga
     7261 ttacgcctcc ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag
     7321 cctcgggctg gcagatttcc agaagcgcat gaccgtgcag gaactgtcga aagaaggttt
     7381 ctccgcgctg gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga ccgcccataa
     7441 aaatgccgtt actttacgtg ttaacgccct taaggagcaa gcatgagcat cgtgactatt
     7501 accgatttag cgcgtgaaaa cgtgcgcaac ctgacgccgt atcagtcggc gcgtcgtctg
     7561 ggcggtaacg gcgatgtctg gctgaacgcc aacgaatatc ccacagccgt ggaatttcag
     7621 cttactcagc aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt gattgaaaat
     7681 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     7741 ggtattgaac tactgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc
     7801 ccgccaacgt acggcatgta cggcgtcagc gccgaaacca ttggcgtcga gtgccgcaca
     7861 gtgccgacgc tggacaactg gcaactggat ttacagggca tttccgacaa gctggaaggc
     7921 gtaaaagtgg tctatgtttg cagccccaac aacccgaccg ggcaactgat caatccgcag
     7981 gattttcgca ctctgctgga gttaacccgc ggtaaagcga ttgtggttgc cgatgaagcc
     8041 tatatcgagt tttgcccgca ggcatcgctg gctggctggc tggcggaata tccgcacctg
     8101 gctattttgc gcacactgtc gaaagctttt gctctggcgg gccttcgttg cggatttacg
     8161 ctggcaaacg aagaagtcat caacctgctg atgaaagtga ttgcccccta cccgctctcg
     8221 acgccggttg ccgacattgc ggcccaggcg ttaagcccgc aggggatcgt cgccatgcgt
     8281 gagcgggtgg cgcaaattat tgcagaacgc gaatgcctga ttgccgcact gaaagagatc
     8341 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     8401 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     8461 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     8521 gtcattgacg ccttacgtgc ggagcaagtt taatgagtca gaagtatctt tttatcgatc
     8581 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggatcgt tttgacaaac
     8641 tcgcctttga accgggcgtg atcccggaac tgctgaagct gcaaaaagcg ggctacaaac
     8701 tggtgatgat cactaatcag gatggtcttg gaacacaaag tttcccgcag gcggattttg
     8761 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     8821 tgctgatttg cccgcacctg cccgccgatg aatgcgactg ccgtaagccg aaagtaaaac
     8881 tggtggagcg ttatctcgct gagcaagcga tagatcgcgc caacagttat gtgattggcg
     8941 atcgcgcgac cgatattcaa ctagcagaaa acatgggcat tactggttta cgctacgacc
     9001 gcgaaaccct gaactggccg atgattggcg agcaacttac caaacgagac cgttacgccc
     9061 atgtagtgcg caacaccaaa gagacgcaga ttgacgttca ggtgtggctg gatcgcgaag
     9121 gtggcagcaa gattaacacc ggcgttggct tctttgatca tatgctggat cagatcgcca
     9181 cccacggcgg tttccgcatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc
     9241 acaccgtcga agataccggc ctggcgctgg gtgaagcgtt aaaaattgcc ctcggcgata
     9301 aacgcggtat ttgtcgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     9361 cactggatat ctctggtcgc ccgcacctgg aatataaagc tgaatttacc taccagcgcg
     9421 tgggcgatct cagcaccgag atgatcgagc acttcttccg ttcgctctct tacaccatgg
     9481 gcgtgaccct gcacctgaaa accaaaggta aaaacgatca ccaccgtgta gagagcctgt
     9541 tcaaagcttt tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgat accctgccct
     9601 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     9661 ctcggtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     9721 cgtcgtgttg ctggccgata aactattttt acccggcgtt ggcaccgcac aagcagcgat
     9781 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     9841 aggcatctgc ttagggatgc aactgctggg gcggcgcagc gaagagagca acggcgtcga
     9901 tttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc taccgctgcc
     9961 ccatatgggc tggaaccgcg tttacccgca ggcaggcaac cgcctgtttc aggggattga
    10021 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
    10081 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
    10141 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
    10201 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
    10261 cagggcgatt acggcaaaca gcgcgattac ggtaacgacc cgctgccgcg cttacaggat
    10321 tacgccacgc agggtgccga agtgttgcat ctggtggatc tgaccggggc aaaagatcca
    10381 gcaaaacgtc aaatcccgtt gattaaaacc ctggtcgcgg gcgttaacgt tccggtgcag
    10441 gttggcggcg gcgtgcgtag cgaagaagac gtggcggcgt tactggaagc gggcgttgcg
    10501 cgcgtggtgg tcggctccac cgcggtgaaa tcaccagaaa tggtgaaagg ctggtttgaa
    10561 cgtttcggtg ccgatgcctt agtgctggcg ctggatgtcc gtattgacga gcaaggcaac
    10621 aagcaggtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
    10681 gaaacctatc tgcccgtcgg cctgaaacat gtgctgtgta ccgatatctc gcgcgacggc
    10741 acgctggcag gttctaacgt ctctttatat gaagaagtgt gcgccagata tccgcaggtg
    10801 gcatttcagt catccggcgg cattggcgac attaacgatg ttgctgccct gcgtggcact
    10861 ggcgtgcgcg gcgtaatagt tggtcgggca ttattggaag gtaaattcac cgtgaaggag
    10921 gccatcgcat gctggcaaaa cgcataattc cgtgtctcga cgttcgtgat ggtcaggtgg
    10981 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgccg ctggcaaaac
    11041 gctacgccga agaaggtgca gacgaactgg tgttctacga tatcaccgct tccagcgatg
    11101 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgttct
    11161 gcgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
    11221 ataaaatttc catcaactcg cctgcgctgg cggatccgac gttaattact cgcctggcgg
    11281 atcgttttgg cgtgcagtgt attgtggtgg gtattgatac ctggtacgac gctgaaaccg
    11341 gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcagtggg
    11401 aaacgctcga ctgggtacag gaagtgcaaa aacgcggtgc cggagaagtc gtcctcaaca
    11461 tgatgaatca ggacggcgtg cgtaacggtt acgacctcga acaactgaaa aaagtgcgtg
    11521 aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa cacttcctcg
    11581 aagccttccg tgatgccgac gttgacggcg cgctggcagc ttccgtattc cacaaacaaa
    11641 taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
    11701 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggac ttatgccggt
    11761 gattgtgcaa cacgcggtat ccggcgaagt gttaatgctg ggctatatga acccggaagc
    11821 cttagacaaa accatagaaa gcggcaaagt cgccttcttc tcgcgcacta aacagcgact
    11881 gtggaccaaa ggcgaaacgt cgggcaattt cctcaatgtg gtgagtattg caccggactg
    11941 cgacaacgac acgttactgt tactggtgaa tcctatcgga ccaacctgcc ataaaggcac
    12001 cagcagctgc ttcggcgaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
    12061 gctcgccgaa cgcaaatctg ccgacccgga aacctcctac accgccaaac tgtatgccag
    12121 cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
    12181 gacagtacat gaccgctttg agctcaccaa cgaggcgtct gatttgatgt atcacctgct
    12241 ggtgctgttg caggatcagg atctggactt aacgacggtg attgagaacc tgcgtaaacg
    12301 gcatcagtga gttgcggggt aagcggatgc gatattgttg tagcatccgg aaaaaaaaac
    12361 gggcaaggtg tcaccaccct gccccttttc tttaaaaccg aaaagattac ttcgcgttgt
    12421 aattacgcag agcgttacgc cccaagacaa tccccgcgcc aaccatgccg cccagcagca
    12481 ccgccagaat caaggtaatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt
    12541 tcattacata gcggtaagca tgaatatcaa gtttatcaac gtcaagattg tcgatatcca
    12601 gcaggttttg acgagtctga tagtagtttg gtgagaacac caacggacga gtcgcttcat
    12661 gcttaatcat cgactccagc gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat
    12721 cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
    12781 cctgaatctg acggatacgc agatctttct gctcctgcgc aactacttcc tgagttttca
    12841 gagaatcctg caagtttttc atacgcagcg cgatgttgtc tttcaggtct tttcccagtt
    12901 cttcatttac ctggtcgtca acttgctgga tatacttcgc cagctgcttc tgagcacctt
    12961 caggtgtttc accaacataa gacaccgcta aaggtaatga ctgatttttg acggtaggct
    13021 caatggtcag cttttctgct tcttcctggt tatccagcgt ttccgctaat gctgagaatg
    13081 cagtgctgta acgtccgata agtgaagcct gaatgtcgga gactttcggt acagccgatc
    13141 cgtagatcac attcatcgcg ttggtataac cagcgatttg ccccacatca ggttgagtaa
    13201 cgatagctgt ggaagtccac ttttctttgg caacggccag ataccctata gccagggcaa
    13261 tagcaacaat gacagagatg ataattgtca tcttgccacg ccacaactgc accagtaaat
    13321 caatcaaatc aatctgttcc ggatcatggt tttgcccaga aacattatta ttttctactc
    13381 tcatcattac cctaacggac gaaataattt taagtcgcca tagtttagcg ataatcgcca
    13441 tgaatgctat aggataaatg ataaaaataa tgaattatta caaagggaac ataagagaat
    13501 cagggcgaaa atcgccatga taacaggatg ttagtcactg ccaaagagat cgcgggtgta
    13561 gactttgtct gccacatcct taagctcttc tgccatacgg ttggagataa tgacgtcggc
    13621 ttgtatagtc agcttagaac agctttgatt gtaaaatgag cacgttacca gaaaaaacag
    13681 ccaagtttag aactgatacc gattatcttt ttttcttgtc taacggtata atttaacttt
    13741 cagttatgcc agatgaagat tggctatatt ctagcctgag cgaggattat aaatgaaatt
    13801 tctggttacg ggagctgctg gctttatcgg tttctatcta agtaaacggc ttcttgcagc
    13861 tggtcatcag gttgtaggca ttgacaactt aaatgattat tacgatgtca acctcaaaca
    13921 agcacgactt gatttactca agcacgacaa cttcagtttt tataaaattg acctggccga
    13981 tcgtgagaaa atggcggcac tgtttgcaga cgagcggttc gaacgcgtaa taaacctcgc
    14041 tgcgcaagcc ggtgtacgtt actctcttga aaatcccaat gcatatgcag atgcaaacct
    14101 gattggattc ctgaacatac tagaaggatg tcgccataat aatgttcagc atctacttta
    14161 cgcttcctcc agttctgttt atggcatgaa ccgcaagatg cctttctcta cagatgactc
    14221 tgtagatcat cctgtttcgc tttatgcagc aactaaaaaa gcgaatgaac tcatggccca
    14281 tacatattct catttgtatg gcttaccgac cacagggctg cgtttcttta cggtttatgg
    14341 tccatgggga cgtccggata tggcattatt taaattcact aaagccatgc tggaaggtaa
    14401 aagcattgat gtttacaact tcggcaaaat gaagcgtgac tttacttaca ttgatgatat
    14461 tgccgaagct attattcgct tacaggatgt tattccagaa aaaaacccac agtgggctgt
    14521 agaaacaggc tcgcctgcaa caagttcagc accatatcgt gtctataaca ttggtaatag
    14581 ttcgcctgtg gagttgatgg actatatcaa tgcgttagaa gaggctctgg gtattgaagc
    14641 caacaaaaat atgatgcctc tccaacccgg tgatgtactg gaaaccagtg ctgatacaaa
    14701 agcactgtat gacgtaatag gattcaaacc tgaaacgtca gttaaagaag gggtaaagaa
    14761 ctttgtagaa tggtatcgta acttctataa agtttgattt tacaaaacca taagaaaagg
    14821 ccctaattta ttagggcctt ttcttagaat gaaacaaaat aattaatcat tgccaaacaa
    14881 gtcgcgcgta taaactttat ctgctacatc agccagatcg gcagacatac ggttagaaat
    14941 aataacatca gcttcttgtt tgaacgcatc cagatcacgt accacgcgcg accggaaaaa
    15001 atcgtcctct ttcatagctg gctcataaac gattacaggc acacctttcg ccttgattcg
    15061 cttcataata ccctgaatcg aggaagcacg aaaattgtct gaaccattct tcataatcaa
    15121 acgatagacg ccaacaactt tcggtttacg tgcaaggata gaatcggcaa taaaatcttt
    15181 gcgcgtgcgg ttggcgtcaa caattgccga gatcaggtta ttcggcacag actggtaatt
    15241 tgccagtaac tgcttagtat ctttcggcag acaataacca ccataaccga atgacgggtt
    15301 gttgtagtga ttaccaatac gcgggtcaag gcatacgccc tcaataatct ggcgtgaatt
    15361 aagtcccagg ctttcagcat aactatcaag ttcattgaaa tacgctacac gcatcgccag
    15421 ataagtgttc gcaaaaagtt taatcgcctc agcctcggtt gagtcagtaa acaatgttgg
    15481 tatgtcttgc ttaatggcgc cttcctgtaa taacgcagca aaacgtttag cgcgttcaga
    15541 ctgctcgcca atcacaatgc gtgatgggtg taagttatca taaagtgctt taccttcacg
    15601 caaaaactca ggcgaaaaga tcacattttc aataccaaaa cgttctttaa tggactctgt
    15661 aaaaccaaca gggatagttg attttataat cattaccgcg ttgggattaa tttctgtcac
    15721 atcacgaatg accgcttcca cgcttgaggt attaaaataa tttgttttcg gatcataatc
    15781 ggtaggtgtg gcaataataa cgtaatcggc atttttatac gcgtcatact tatctgtcgt
    15841 agcgcggaaa ttgagatctt tagtcgccag atactcttca atctccttat caacaagcgg
    15901 tgactgcctc ttgttaagca tgtccacttt ggcctgaacg atatccagtg caaccacttc
    15961 gtggttttgc gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt
    16021 tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac
    16081 cacagcttca tctactgcgg gatttttaac gctgatgtag atgaactgtc aaggcagcga
    16141 tcctgctgtg cggcgctgta ttatatcgcg tttttaacta taaattataa aaaaaggccc
    16201 taacctgccg ctttgtataa taaaaaagcc cggagggttt ctccgggcct tgttttgatt
    16261 aattgattta aatcagatta atccagccat tcggtatgga acacaccttc tttatcaatg
    16321 cgcttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    16381 acagcggcgc ggtagctgtc gtaataggca accgcagcgg cgaaggtcgg caccgggata
    16441 ccgttctgta ctgcgtaagc gacgacatcg cgcagcgcct gctggtagtc atcggcaatt
    16501 tgcttgaagt aaggagccag caacaggtta gcgatctgcg gattttcggc ataagcatcg
    16561 gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    16621 tcaccgtagt tcagatccca gttgtactct tcagacgcag cgcgtagctg agagaagccc
    16681 tgagcgtaag aaacgatttt gcccagatac agcgcacggc gaactttttc gatgaactca
    16741 gcattgtcgc cagctggctg cgcttgcggg ccagagagaa ctttagatgc ggcaacacgc
    16801 tgctctttca gagaagagat ataacgtgca aacacagact cggtaatcag cgacagcggt
    16861 tcgccgagat ccagcgcgct ctggctggtc catttgcccg tacctttgtt tgctgcttca
    16921 tccagaatca catcaaccag gtagttaccc tcttcatctt ttttggtgaa gatatctttg
    16981 gtgatgtcga tcaggtagct gctcagttca ccgttattcc actcggtaaa ggtctgcgcc
    17041 agttcttcgt tggtgaggtt caagccacct ttaagcagag aataggcttc agcaatcagc
    17101 tgcatatcac cgtattcaat accgttgtga accatcttca cataatgacc tgcaccatcg
    17161 gcaccaatat aggtaacgca cggttcgccg tcttcagcca cagcggcgat tttggtcagg
    17221 atcggcgcaa tcagttcata agcttctttc tgcccaccag gcataatgga aggacctttc
    17281 agcgcacctt cttcaccacc ggaaacaccg gtaccgataa agttaaagcc ttctgcagaa
    17341 agctcacggt tacgacgaat ggtgtcatgg aagaaggtgt taccaccatc aatgatgatg
    17401 tcacctttat cgaggtatgg cttgagggaa tcaatagcag catccgtgcc agcacctgct
    17461 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaattc tttcaccgta
    17521 tagtaaggaa ccagtttctt gcctggattt tcggtaatca cttcttcggt cttttcacgg
    17581 gaacggttga aaatagagac ggtataacca cggctttcga tattgagcgc aaggttgcgc
    17641 cccatcactg ccataccgac gacgccgatc tgttgctttg acattgttta ctcctgtcag
    17701 gataccgctg ggtggtatgc gggttatgct taattataga atatgcctaa taaaaataaa
    17761 tccataacac ttaatcagaa aattattatt atcgattcct aacgattgaa tacatcagct
    17821 cctttaattt agatggcatt atacgaaaaa atgttctcaa catagcatta cttattaatt
    17881 cattttttcg aataaaacca attttatatt gataatacaa tactttatac tcgtacaata
    17941 aatatgacaa tccacgtcga gccataagat tacgaccagt tcgcattttt aataaaatat
    18001 ctggaagatt tgcaaatctt gcattatgta caattaatag gctccacaat gcaaaatctt
    18061 gagattttct gaatggagga taaccaccaa cagctaatac tgtattcttt ctaaaaatta
    18121 cagaaggatg gctaactgcg cttcgtttcc tcgcgaattt aactatttct ctatgttcga
    18181 gaggcacttt gcgtgttgaa ataaactcct cagtaacagt ttcaatttca tcaataaaac
    18241 tgccacatac atctatttct gaattattaa tcataaaaga aatttgtttc tcaaaccgat
    18301 gaggcaaaga aatatcatca gcatccattc ttgccactaa ctcattccta caagccttta
    18361 atccttcatt taaggcatta gccaatccaa catttctagg taaaggtaca aatgttacta
    18421 ttttattgcc aacatcatca atgaatgaat ttataatatc gatgtgtgtt tgatggagtt
    18481 ctccatctgc aacaattact atttgatctg gcttaagtgt ttgatcgtga aaaatagagc
    18541 gtagagccac ctcaaaaaat tgcggtagat catttttata aatgctaatt aaaactgaga
    18601 atttttctaa tctatgattc atttcatttt accacttcga cccattaaac cgtcattaat
    18661 gccttttaaa aaaaaatata accttttatt accatttgga aggaaaatag gatataaaaa
    18721 aacctttcca attaatttaa ccagactaga aattttccag tagatgggta cataattttt
    18781 atttaataaa agaaagatat ttcgagtagc ataataatga cgaaatgggc ttggcaaacc
    18841 gacagaaaga atatttaaga tcttaaatcg cccatctcca agtctatgtg caagtaacgc
    18901 atttttattc ctaattactt taaacccagc agctcttaat ctccaacaat attcatggtc
    18961 taccgcatcg ataaaaagct catctttcat tcctccaaca atcaaccaac tattttttgg
    19021 tattagactg ccagaactta atgtactatc tacctcataa taaacttctg taagtggttt
    19081 cccttttttt acccttgctt tatttaattc accagttact ttatcaaaat cttgtgaacc
    19141 aactaaacca acattgacat tttgtttaag caattttttg taacaagtaa gtaactgctc
    19201 taccatctta ggatcaggaa tactatcctg atccatttgc aatataaaat cagcgccatt
    19261 ttcaaaagcc catttcattc ctatactttg ggcttctgct atgcctaaat tatcattgaa
    19321 attgaatatt tttacatcgc ctgaagaatt ttcagcatat ttataaccat ttgtagagtt
    19381 attgcaaacg acaactttag taacttgtct caacaataat tcaaccgcat tttttaaatc
    19441 attatgttct gggttgtaag caaccaaaac ggcatataca gtgtccatct tcaccttaaa
    19501 accttcattt agctttcatc ttttttagaa cattacttaa tgtcactaat acaattatta
    19561 cagcaacatg gttagagtct aaaatataag gattagtaat tgcataagaa acatatagaa
    19621 aatatagcac acacaactca ctgtatttta tgattttaat cgtgagaagg agattaatta
    19681 ataaaaacaa agtaaataaa ataacgccaa gttgatttaa aaaataaact gactgcaatt
    19741 cataatatat atatgcacta taatcacgga taggagtttg aattttgatg acattaccca
    19801 aaccagaacc tataacaaaa tttgatacag actctgtaag atcattaatt aatacagtaa
    19861 actgatccca tctaactcct aaagaagaat cagctccatt tgatttcatg attatcaact
    19921 caattgaata tgtaataaaa aaagggagaa tcacagtaag aaaaacccca aaaataattt
    19981 tccttaattt agcgtatcgt gagttagatt tagaacatag tataatatac ataaaaaaca
    20041 agcatatcga aacaaaatat gcaaaattac cagccactat agtacctata gccagaataa
    20101 cggttattgt atttttgaat cgataataga aataatcttt tatgactata tgcaacataa
    20161 aggcaaatgg aatgagagca tttcctttaa tttgaactct atagaaacca cttccatatg
    20221 tataaacatc accataatca ttctccaaaa aataatgtct tagtgctgaa taatcaccaa
    20281 taccatatgt ttttgtcata taaatactaa tgatggatat aataaccgcc tgtaatacca
    20341 ttaaatataa aaatatttta acaatcgaga tggttccata agagcagaaa taagcacata
    20401 atataaataa tatgataata taaaacctaa ttattatcgc tatatcgtta cccttgatat
    20461 aggaataaat aaaatttata aaaagagcta atagaaatat taaaataaca ggatagtgat
    20521 atattccgtt tgcaattttc tttgtaaatg acatgataca aagacataaa aacccctcca
    20581 taatccaact atattgaata aatggaaagc tacgtgtaag gaaaaatata aacccaaaaa
    20641 acaaaagaac acttaaactt ttgtcttttg agttataaaa atcagaagtc atgtttgcac
    20701 tctaattaga tgggcttgag gaagtaatcc ctaaaatcaa ttcgctatta atatttcgta
    20761 tcaattaata ataatatcaa aaaatctaac gatgttctta cagaccatgc tattgcggct
    20821 ccaacaattc cccaatgata aataaaaata tataatatgc ataaatatgg gataacttcg
    20881 agcaaatgaa taatagctgt aatttttgat cttccactag cctgaactga aacaaatggg
    20941 atttgtgcaa tgcaattaaa aaagaaacct attgcaagaa tttttaatac tatacctggc
    21001 gtcccatgat atgtaggtcc catccaagcg gacattataa aatctgataa aataattatc
    21061 aacattacaa ttggaagtat accaataacc attataaaat atgataatat tttagtttgc
    21121 tttaccgatt gcaattctga acttaatctt ggaaaaatag ctctggacaa cgcacttggt
    21181 aatatcgtta agcgttgtat accttcagac ggagcagtat aaaaagaaac tttatcagcc
    21241 cccacaatgt gtgaaagaat aaaacgatcc atatatgtca taatagggct aataatattg
    21301 ctaactgtta tccagcttcc aaagccgatt aatcttttaa ctgttacaat ttttacagac
    21361 agcccagatg atattattag ttttcgacta aatataaagg tcactataag tgataagact
    21421 cttgccataa ctaaaccata tatagcactt agtaatcctc catgaaaaaa acagaaaatc
    21481 actggtaatc cagccacaaa agagttgtta attgatttta ttaaatttac ttttctgaac
    21541 ttttccatcc cctcaaaaat ccccaaccag acttggttta acaagtataa gggtatggta
    21601 gctgaaataa tatatattgc tttgacagat tctacaacat gattcgcgtt aatgtttaat
    21661 aatttaacaa ttacattgct actcaaaaat agtacactac cgccaatcaa gcccaatata
    21721 gttagaatta ccgttgaagt tgaaatgatc gctcttaatt ctttatgaac atttttatat
    21781 attgatactt ctcttataac agctctggtc aatccagcat caaaaatact tgcatatcca
    21841 actaaggcaa tagctaacgt aaaaaggcca aattgctcgg tccctagaat tctagacagt
    21901 atacctaacg caggaattgc tattaatgat ggtataatat acccacttat attccataaa
    21961 gtattcttta caatactcac aaaaataatt ccttcatgtt atgcaattct ttagcccttg
    22021 catctttaat cgataaaata taattattat gttctatcgt cggccatttt atgctcagaa
    22081 taggatcatt ccatacaatc cctctatcac tatcaggatg ataatagttc gtcgttttat
    22141 ataaaaattc cgcagtctcg ctcagcacca aaaaaccatg tgcaaatccc tcagggatcc
    22201 acaattgccg cttattctca gcagataaat tcaccccaac ccatttacca aaggtaggcg
    22261 acgatttacg aatatcaaca gctacatcaa aaacctcacc aacaacgcaa cgtaccagtt
    22321 tcccttgcgc ataaggttct aactgataat gcagcccgcg taaaacacct ttactagact
    22381 tcgaatggtt atcctgaaca aattcaacct tacgtcctac agcttcttcg aaaactttct
    22441 gattaaagct ttccataaag aaaccacgct catcaccaaa aactttcggc tcgaaaatta
    22501 acacatcagg aatttctgtt ttaattacgt tcattttatt aataaccttt aatcattttc
    22561 agcagatact gtccataagc attttttttc agcggctccg ctaatgcttt cacctgttca
    22621 gcatcaataa accctttacg gtaagcaatt tcttctgggc aggaaacctt tagtccctgg
    22681 cgctcttcaa tggtggcaat gaagttgctt gcttcaataa gactctgatg tgtccccgta
    22741 tccagccatg cataaccacg ccccatcatg gcaacggata aacgcccctg ttccatataa
    22801 atacggttaa tatcggtaat ttccagttca ccacgggcag aaggcttaag gtttttcgcc
    22861 atttcgacaa cgtcgttatc atagaaataa agcccggtta ccgcataatt actttttggt
    22921 tgtagcggtt tttcttccag gcttattgcc gtaccgtttt tatcaaactc aacgacgccg
    22981 tagcgttcag gatcattaac gtgataggca aataccgttg caccactttc tttgttaaca
    23041 gcgacatcca ttaacttcgg cagatcatga ccgtagaaga tattatcacc aagaaccaaa
    23101 gcacaatcat caccaccgat aaactcttca ccgataataa acgcctgcgc aagcccatct
    23161 ggagtcggtt gcactttgta ctgaagattt agcccccact ggctaccgtc acctagcagt
    23221 tgttgaaaac gaggagtatc ctgtggcgta ctaataatca gaatatcgcg aatacccgcc
    23281 aacatcagtg tagagagcgg gtaatagatc atcggcttat cataaatagg taatagctgt
    23341 ttactgacag ccatagtcac aggataaaga cgtgtaccag aaccacccgc taaaataata
    23401 cctttacgcg ttttcatttc atcattcctt ttaattcatc ttgctccacc atcacgaaca
    23461 agatgcaaaa actattaaat tgctgtagtc gtaattaatt cgttgagcat tcgtttcaca
    23521 ccaacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc tgtattaagg
    23581 cgagagttat gtggacgacg agctggtgta ggataggctg ttgttggtac tgcgttgagc
    23641 ttgttgagtg caaggggaat acctgctttg cgcgcctctt caaaaaccag cgcagcataa
    23701 tcgtgccagg ttgtggtacc actggctacc agatggtaca aacctgcgac ttccggttta
    23761 ttcagtgcca cacgaatagc atgtgccgta caatcagcca gcagctcagc acctgttggc
    23821 gcaccaaatt gatcatttat cacagccagt tcttcgcgct cttttgccag acgcaacatc
    23881 gttttggcga agttatttcc tttagctgcg tatacccagc tggtacggaa aataagatgc
    23941 ttcgcgcaat gttcctgtaa cgctttttct ccggctaact tggtttcacc gtaaacattt
    24001 agcggtgcgg ttgcatccgt ctccagccat ggcgtgtcgc catttccagg gaatacgtag
    24061 tcagttgagt aatgaattac ccaagcccca acttcattag cctcttttgc aattgattca
    24121 acactagtcg cattgagtaa ttgtgcaaat tcgggttctg actcagcctt atctactgcg
    24181 gtgtgagccg cagcattaac aataacatca ggtcgaattc ttttgactgt ttcagctaca
    24241 ccttcaggat tactaaaatc accacaataa tcagtggagt gaacatcaag agcaatcaaa
    24301 ttacccaaag gtgccagagc acgctgtagt tcccaaccta cctgccctgt tttgccgaaa
    24361 aggaggatat tcattactgg cggccctcat agttctgttc aatccacgat tgataagcac
    24421 cacttttcac attatcaacc cattttgtat tggacaggta ccattccaat gtcttccgaa
    24481 tcccgctctc aaacgtttcc tgcggtttcc agcccaattc gcggctaatc ttctctgcat
    24541 caatcgcata acggcgatcg tgtcccgggc gatcggcaac ataagtaatt tgctcgcggt
    24601 aagatttctc tttcggtaca atctcatcca gcaaatcaca aatagtgagc actacatcga
    24661 tgtttttctt ttcgttgtgt ccaccaatgt tataagtttc acccgcttta ccttcggtta
    24721 cgacggtata taacgcacgc gcatgatctt caacatacag ccagtcacga atttgatccc
    24781 ctttgccata aataggtaat gccttacctt ccagagcatt cagaataacc aatggaatca
    24841 atttttccgg gaaatgataa ggaccataat tattagagca attagtcaca atggttggta
    24901 aaccataggt acgtttccac gcgcggacta aatgatcgct ggatgctttt gaagcggaat
    24961 aagggctgct tggcgcgtaa gctgttgtct ctgtaaataa gggtaattct tctgtattat
    25021 ttacctcgtc aggatgaggc aaatcaccat agacttcgtc agtagaaata tgatgaaaac
    25081 ggaatctagt tttcttgtcg ctatcaagag cagaccaata attgcgagcg gcttccaaaa
    25141 ggacatatgt accaacaata ttggtttcaa taaatgccgc aggacctgta attgaacggt
    25201 caacatggct ttcagcagcc aggtgcatca ctgcatctgg ctgatgctga gcaaaaatcc
    25261 gtgccattgc agctgcatcg caaatatccg catgttcaaa aacatagcgt tcagaatcag
    25321 aaacatcagc aagtgattcc aggtttccgg cgtacgttaa tttatcgaca ttaacaacac
    25381 tatcctgcgt attatttata atgtgacgaa ctacagcaga accaataaat cctgcgccac
    25441 cagtaacaag tattttcacc taatttattc catattgctt cagagcatgc tgtgaaataa
    25501 gcggctctca gtttgattaa tagaagtatt aatgcacgct accgcccctg gctttacagc
    25561 taccagagca ctgcatgcat gcctacgatg tgacgagcgt tacccactcg cgctaaaccc
    25621 gaaaaattca aaagctaatt gtcttaccaa tccgctctgg aaacaaggaa aatcctggaa
    25681 aactttgact aaaatcctat tgctaactcg ttgttattct gattgtttat ataaaacaac
    25741 ggcaggaata ttcgcaacaa attactttca ccacgaatct tcactgccgt tataattttc
    25801 ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc tcaatacctt
    25861 tacggaactt cgccccttct ttcaggttgc gcagcccata cttcacaaac gcctgcatat
    25921 agcccatttt tttaccgcag tcgtagctgt cgccggtcat cagcattgca tcaacggact
    25981 gttttttcgc cagctcggca atggcatcag tcagctgaat acgtccccat gcaccaggct
    26041 gagtacgttc aagttccggc caaatatcgg cagaaagcac atagcgacca acggccatga
    26101 tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca atgcggctga
    26161 ctttaccttc gcgatccagc ggttctttgg tctggatgac ggagtattca gagaggtcac
    26221 ccggcatacg ttttgccagc acctggctac ggcccgtttc attgaagcgc gcaatcatgg
    26281 cagcaaggtt gtagcgtagc gggtcggcgc tggcgtcgtc gatcacaacg tctggcagca
    26341 ccacgacaaa tggattgtca ccaatggcgg gtcgtgcaca caaaatggag tgacctaaac
    26401 ctaaaggttc gccctgacgc acgttcataa tagtcacgcc cggcgggcag atagattgca
    26461 cttccgccag tagttgacgc ttcacgcgct gctcaaggag agattctaat tcataagagg
    26521 tgtcgaagtg gttttcgacc gcgttcttgg acgcatgagt taccaggagg atttctttga
    26581 tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg acgatcggta
    26641 gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccaaga cccgctaccg
    26701 gtataactgc ttttaaattc gtcattattt tcctacctct aaggggctga tagtgcgtaa
    26761 attattgtca taggttagcc aaacggtatg gctatatacc aagcataact ttgattaaac
    26821 cttacgataa cactacacac catcagcatc tgggttactc ggattactcg gaaatccaca
    26881 tactgataat ttaatcagta cctctttccg aataatcgta gtccaacctg gtcctttttt
    26941 ctctgactcg tctgcattac tcagaaacaa acgttatgtc gtcttttttg gcatggacga
    27001 attcatactg cagagttcga tccagacctt gcgacagcgt atacggtgca acaaaacctg
    27061 aagaatgcac tttcgttgcg tcaaactgtg ttgttgcgca gaattttttc acgcgcacag
    27121 agctgacagc gtattttttg cccgtaattt tgctcaggat atcaaagcaa tatccaccca
    27181 gcattcctag tgggtaaggc aagtgcatag aagggatctt tttgttcagg ctttgttcaa
    27241 cttcagcaac caactggttc atgttcaggt ctggcttatc aacatagtta taaacctcat
    27301 aacctgcggc aacattcttc agtttgtact tgataaactc aacaatgttt ccaacataag
    27361 ccatggactt atagttagtc cctgcgccca ccatcataaa cttgccgcca gcgatctgtt
    27421 tcagcaagtt atagacgtta ccgcggttgc gttcaccgaa gataacggta ggacggatga
    27481 tggttaatga acgttctgtt ggtgctttgt tataccattc acgcagcact tcctctgcct
    27541 gccacttact tttgccgtag tggttgaaag ggtcgtgtgg atggttttcg tcagggttgt
    27601 gtttgttcaa accataaaca gcaacggaac tggtaaagat gatattttta acgccatttt
    27661 tttccatggc cgccagcaca ttgcgggtac cctgaacgtt gacatcataa tagagagaag
    27721 tagggctgac gtcatcgcgg tgttccgctg ccagtagtac aacagtgtca aaaccggcta
    27781 acgcctggtc gagtgcctgt tgatcacgaa catcaccaat ctgtgtgatt tctggataaa
    27841 agtggctctg ccgtttgtcc aggttcttga tattaaagtc agcaattgcc gtttcaagta
    27901 gtcgggttcc tacgaatccg gaagctccta tgagcaaaac gttattgttc ataaatcact
    27961 ttagtctggt tgttacgtaa gaaacacaag ataaagatga gtaccttccc tgagtagtca
    28021 atgctgccca gccccagctt taacagttag tgtgaggatt ataatctttt agaacattat
    28081 atccagtaag tttatgaatg gtcgcaaatc tactctctcc gttccggcaa tctaaagtta
    28141 atcttttcca cattaacaat atggtgatta atcctgtcga tgtcgacgga gctttgccct
    28201 ctttcattca tcgcatgaac atttgcaagt gacagcagtg tttctttttt cgccataaag
    28261 acaccgcgta cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    28321 tcctgcatca ccttgatatt acgcataaaa agatgttgcg gtttgttgtg taactccagc
    28381 gaggcacgct tcatctcaat gttagtcagt gccacaaagg agacagcatt cccggcggag
    28441 atttggatgc cgcgcaattt ataaacaaga tgggtgttat ccagttgaat attattcact
    28501 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    28561 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagca
    28621 actgtcgcgt tatcaatgcc tgctttctta ctgaaatccg gcgtgatatt gcgggctttg
    28681 atattacgga taacaaaatg tttgccattt tcaacatgga tcaactgccg acaatccgat
    28741 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttattatcg
    28801 taggtactcc ccgcaaggcc tatgccaatt ccccagttga ttttgccatt agtacagttg
    28861 atgcgctcga tgacatggtc ggagatcaaa atatcactgt cgttaattgc cacgttccat
    28921 tcaatggcgt cgccctgtaa gtcgctgaac ttacaattgg tgatgttggc accgataatc
    28981 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    29041 atggtcaggt tgctcatgac ccgtttgttt ttgccgccga tataaatctg cgttaccggg
    29101 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aaccacgcac atccaaggtg
    29161 atgttatgca tactgccgcc ctcctcccct gtcacctggc tgccgtcctg taagacaaat
    29221 cgccctctgc cgttgccgcg caagcttcca agaatgtgta acgttttacc gggagggata
    29281 aagatgccgg tgttgatatt gtcacaaacc aatccggcag gcacgacgac cgtatgccct
    29341 tcgctgaagg cttgtttaaa tgaggcgatc cagtcgtgtg ggttgtagtc gttaatgtta
    29401 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    29461 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatgcgttc tcctctataa
    29521 agcttgcagc aagctggcga gttctcgatt gatcacctgc tggttaaaat cgtgttcgac
    29581 tttttcgcgc gcacgtttga caaccggagc cagttcgtcg gtgcccagtt ggctaaacgc
    29641 cgccagtcgt tgcgccagtg cgcgagcatc gttctcaggc accagccagc cggatttgtc
    29701 agcctccacc agttccggta ttccgctatg cagagtagaa accaccggaa taccgaccgc
    29761 catcgcttcc attagcgcca ccggaatgcc ttccatatca ccatccgcgc ccgttaccga
    29821 tggcaacaga aagacatccg cgtcgtcgag catcgctttc acttcgtggc tcggtttaaa
    29881 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtac gcaggcgtcg
    29941 ttcccacggg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    30001 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacggaaat
    30061 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    30121 gcccatacgc gatacggcga ttttttccct cgggcagccc attttttgca gccttccggc
    30181 ccacaaatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    30241 agtgtaatgg ttgagcactt cccgactgga aatatcaatg ccgtggaaaa tggtggcaat
    30301 tttgccgcga atgacaccca gttcccgtag ttttgctgcg gttacccccg ccgggccaaa
    30361 atgagcgatg aacacatcgg catgaagcgg tgttgcgacc tggccgcaaa tggcagacaa
    30421 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcctgcc aggtattttt
    30481 acgataaatg ccgcgtaacg tctggctggc gcggtggcgc agtttcgcta ttttgcccgt
    30541 aggttcgtcc tgtaaccagt gggttctggc ggcaaggttg tatttcgtcc atgccgcgtg
    30601 ggtgttttgt gtgtcgcctt tttgcagcgc gagaatctct acctcaaatc ccatatcaat
    30661 aaacgcggta atttggttaa ggacgaaggt ttctgacgac agcgggaatt tcagtaaaaa
    30721 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    30781 cctgtctgac gctcacgact gacggcttca ctaagtcgcg cattcagtgc cggaagctgg
    30841 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcta ataaatgacg gatatcaatt
    30901 gccatctccg gtagtcccag ctgctgcata atcccggcgg atttatgttc gtagttgatg
    30961 gcaattgccg gagtggcaaa attcatcgag ataatggcag agtgcaggcg cgtaccgacg
    31021 gtgagttcac aggcaccgag aattttgccc atttccagat cgttgagttc atccatcacc
    31081 acatggtaac gggcaggatc gctgatgtgc tggcgaagat tgagcgccat catgcggtcg
    31141 tctttgttat agctgtcgat acccgtacag gtggagagtg caatgacctg atagccttcg
    31201 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    31261 aggcgtttgt caaacggtgc cagttcgcgc agggtaattg ctaccgtttt ctgttgtgcg
    31321 gcaacgtcca gccagtgttg aacagcatag ctggcggtga agtcttcggt gtggtgatcg
    31381 accagccacg cggtatcgac gccatgttcc acttttgcgg tagtgatatt gctgcgtttc
    31441 atcagatcaa gactgaccga ttcgcgcagg atcagcgcgt cacagtgacc aaaaacgtag
    31501 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    31561 agcggctttt tcgccataaa cgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    31621 tcgacaaaaa acgatcctcc gacctgaata atggcgtcgt aacctgagag caggcgcacg
    31681 aagtcggtga atccctgggc gatggcgata ttacgcagct tgccagtgtc ggtgacgcgt
    31741 gagagcaata cctggtgttg atagcggcga cggaggactt ttttaacgcg cccgacaacg
    31801 cccgccgcgc tgttgtgttg tttcatttgc aggaacagag gatcgcccat taccgggcgg
    31861 ttgagtagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggcttgtgga
    31921 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    31981 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaagtg
    32041 aggctgtgtt tgttttgttc gttgccgctt tttgcctgat gcgacgctga cgcgtcttat
    32101 caggcctaca agacccgagc acagaaccgt aggccggata aggcgttcac gccgcatccg
    32161 gcattcagtg cctgatgcga cgctgacgcg tcttatcagg cctacaggtc ccgagcacag
    32221 aaccgtagga tggataaggc gttttacgcc gcatccggca accgttgtcg gaaccgaaaa
    32281 cagcaactca ccccgcccgt aaaagcattt tcattttttc gctgcgacaa aactgacgct
    32341 tcacttccac caccagcgga tggcgcgaca gcacaatcat caccacaaac gccagcacgc
    32401 ccgcggctat ttgcaccgcc agcagcatcc ccagcgccag ttgccctttc agcacaatgc
    32461 ccagcgcata actgaccacc agcgtcggca acgagagata aaacggcagc cacaaactca
    32521 ggatgtactg gcgataactg gaaccaagaa ccggtttaat catcacgaag taactcagaa
    32581 tggtgttgat aatctgcacc agcaggaaac cgagcgtgac gccgattgcg cccgccatct
    32641 gcccaccaat aacaatcgcc ggaataaaca gaaatgtttt gaatacgttg aatttaaagc
    32701 tgatatcgac ccgcgctttc gccatcagca gcgaaccaat cgggttaccc acggagcgca
    32761 gtagccccac cacacacaga aattgcagca ccggaataat gctgttccac ttctcaccaa
    32821 agaccagtgg tacaaagtta ttcgacacca ccattagccc gagcagcgcc ggaaagttga
    32881 taatccccac caccgacagc ggtttgtaga agttaatacg cagcttttcg gtgtcgtcct
    32941 gaattttggc aaacgccgga aacaacacac gggtgatgat tgggttcagc ttcatcggcg
    33001 gcacaacggc cacgttgtaa gccaggttgt agccccctgc cacgcccgcg ccgagaatac
    33061 gcgccagcac cagcgttgaa aggttggtat tgagatagtt gatgatgctg tccgccgtca
    33121 gccaggcacc aaagcgtaag ttcggtgcca ccgacgccag cgagaaatgc agaccggggc
    33181 gataaatttt gcggccaaag tagccaaaca gcagcgttct caccgcacta ttgaccagat
    33241 aaccgaggat cgcggtcatc gccagcggcc agaaatgggc gctaaccacc gtaaaagtga
    33301 agcccgccag taccgcgctg gtttcgatca tgccgatttt gttgaactcc agctcctttt
    33361 gcatcaacgc gcggaactgc tgcccctggg ggatcaccac aaacgccagc gacaatgttt
    33421 taatcaacgg tgccaggtcc gggttattca gcacgtcgcc gatgagatca ctcaacaaaa
    33481 acaccgccag acacaccaca agccccagcc cgacgttcag ccagtacagc gtggtgagtt
    33541 caaggtggct gatttctttt cgctgaataa tcgagttagc gataccgaag tcagaaagcg
    33601 tatctgccag cgcgatgatc accagcgaca cagtaagcaa accgaactgg tggttgtcga
    33661 taatccgcgc cagcacggtc atctgcacca gcccgaggcc gatgatgatc accgtggcaa
    33721 tcgccgacca cttcgcgccg ctgatggttt tttcacgtaa gctcatatca atatgccgct
    33781 ttgttaacga aacctttgaa caccgtcagg aaaacgattt tgatatcgaa ccagacgctc
    33841 cattcacgga tgtactcaag gtcgaactcg acgcgttttt ccattttctc cagcgtgtcg
    33901 gtttcgccgc gccagccgtt aatctgcgcc cagccggtaa tgcacggttt cactttatgg
    33961 cgcagcatgt agccttcaat gagctggcga tactgttcgt tatgcgctac tgcgtgcgga
    34021 cgtggaccga caatcgacat ccccccggtc agcacattga taaactgcgg taattcatcc
    34081 agcgaggtgc ggcgcagaaa gttccccact ttagtgacgc gcggatcgtt ctgcgtcgcc
    34141 tgggtcacca ctttgtcgtt ctccatcact ttcatggaac ggaacttcca cactttgatc
    34201 ggcttgccat ccatgccgta gcgagtctgg cggaaaataa ccggccctgg tgaactgagt
    34261 ttcaccgcca gcgcaataca gcacagcacc ggggagatca gcagcaagat cagcgtcgcc
    34321 agcacaatgt cttccgcacg ttttagcagg cggttaaccc cggaaagcgg cgtgtcatac
    34381 agcggcacca ccggaacgcc gttcatctct tcgagacgtg aatggagaat attgaaggta
    34441 aagacatcgg ggatcagcag caccgaacag gtggtgtccg ccagttgatg gaccagtttt
    34501 ttcactcgcg cgccgtcgca catttgcatc gcgatataga cgttatgaat cttacccgct
    34561 ttagcatcct cgaccagctg ttgcagattg cccgcccagt cgttagaaac accgcccggt
    34621 ttcgggtcgt ggtaaacgcc caccacttca aaccctaacc acggctgatt acggaagctc
    34681 tccatcagca tttgtccggc ggctaaatcc cccgccaccg cgaccatgcg cttgttatag
    34741 ccatgattac gcagccagcc cgccccaatg cgaatacatg aacggcaaac caccagtccg
    34801 atactggtca gcccatacca cgccagccag attttcagtt gcgtgtcgaa atcattgttg
    34861 aacgccacca gtccggcgct gaaaatcacg cttaaggtcc agttttgtag caacagggca
    34921 aattctgtcg ctgcccgaac accgcgccat gagcgataaa aatcggtgat gccgcccagc
    34981 atctggaaca ccaccagcgt aatcaacgcc accaacaggt gcatgtagag gaatgacagt
    35041 ccgctcactt cgcaaaccag ccatagtccg gcaaacatga tggtgatatc tgaaaagcgt
    35101 tgcaccatag agattaacga tgcattggtt ttcgctcgcg cttttttaga tttgtcatcg
    35161 ttgttcctgt tattagcccc ttacccgcag tggggtaagg gaagatccga cattactcgt
    35221 tcagcaacgt cagcagagtt cgcgttcgcg cttccatcag cggcacatca ccgcgtgatt
    35281 ccacattcaa ccgcaccacc ggttcggtgt tggaggagcg cagattaaag cgccagtcgg
    35341 caaaggtcat gctgatgcca tcggtgcgat ccaccgccag cgcatcacgg ctaaaatgct
    35401 gttccacgcg gttaatcgcc tcaacgggtt gcgccagttt gctgttgatc tcaccgcttg
    35461 ccggaaacgc cgccatccgg tcgcgcacca gttcgcccag cgtttttcct ttcaggcaca
    35521 ccagttcggc gaccagcagc cacgggatca tgccgctgtc gcagtaagcg aaatcacgga
    35581 agtaatggtg ggcgctcatt tcgccaccgt agatggcgtc ttccttgcgc atacgttctt
    35641 taataaaggc gtgtccggtt ttcgacatca ccgacgtgcc gccagcggca gtcaccacat
    35701 caacggtatt ccaggagaga cgtggatcgt ggatgatctt cgcgccggga tttttttcga
    35761 ggaacgcttc tgccagcagg ccgacaatgt agtagccttc gataaactgc cctttttcgt
    35821 caaacaggaa gcagcggtca aaatcgccat caaaggcaat gcccatatcc gcgccgtgtt
    35881 tgatgaccgc attgcgggtg tcgtcgcggc attccggcag tagcgggtta ggaataccgt
    35941 tggggaaatt gccgtccggc gtgttgtgca ctttgattaa ttccacgggt gcgccaaggg
    36001 ctttaaagcg ggcttcaatg gcgtccacca ccggacccgc tgcgccattc ccggagttaa
    36061 tcaccagctt gagcggcgtg aggtttttga cattgatata accgaacagg tgatcaacgt
    36121 aagcgtcacg caggttgatt tgctgatagc gaccgcgttt ggtttcatcg acgggaggaa
    36181 agtcgttggc ttcagccaga cgctggacgt cgcgcagtcc ggtatcaccg ctgatcgggc
    36241 gagccccttc gcgcaccagc ttcatgccgt tataatccat cggattatgg ctggcggtga
    36301 cttcaatgcc gccatccacg ccgagatgga acgtggcgaa atagatctct tcggtgccgg
    36361 acataccgat atccagcacg tcaacgcccg catcctgtaa accttttgcc agcgccagtt
    36421 ttaaggtttc gctggtgaga cggacatcac cgcctaacac aatggttttc ggtttgagaa
    36481 attcgccata ggcgcgacca atgcgccagg cgatatcttc attcagttct tcgcctaatt
    36541 tcccgcgaat atcataggct ttaaagcagg ttaatttttt catgtcgtta ccctttttca
    36601 ggcaatagtt ggccctgacc gaaacgacca attttgtttt tgtcgttatt tacatctcgg
    36661 tttcgcattc attgcctgat gcgacgctgc cgcgtcttat caggactaca aacccgaacc
    36721 gtaggtcgga taaggcgttc acgccgcatc cgacaaacag cgcctgatgc gacgtttaca
    36781 cccgtccgta gcgatccgcg aagcgcacca catcatcctc ttcgagataa gagccggagc
    36841 gcacttcaat taaatcaagc ggaattttcc ccggattttc caggcagtgc gtcgccccca
    36901 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    36961 ttgccgttcc cgcgaccact acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    37021 aaccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    37081 agtcatattt gccccacgga cgatacactt cgcgatgcac ccgatgctca tggcgaccat
    37141 cggctttgat ctgctcgacc acttttttca catcctgtac cgcgttacgg tcggcaatca
    37201 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gtgaccaggc
    37261 cagattcggc atacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    37321 cgttgccctc ggcggtgtgg gcgctgatct cccataatga agaccaggaa ccaacatcgc
    37381 tccagcccgc atccatcggc accacaacag catctgccgt acgttccatg accgcgtaat
    37441 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcaagat
    37501 ccggatcgac ggagctcatc gctttttcac aggcatcgag aatatccggg cgatattttt
    37561 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    37621 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    37681 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    37741 aaccggtttc tggtagatcc ggcacaatgc caaaggtcac cagcttgccc gcttcggcat
    37801 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatt acatgatccg
    37861 ccgccagcac cagcattaac gggtcgttct ccgggctatg acgttttgcc gccagcgccg
    37921 ccagcgcaat ggccggtgca gtgttacgcc ctgcaggttc gagaataatg ttctcggtga
    37981 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    38041 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    38101 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    38161 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    38221 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagcgtgc gttcggcata
    38281 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgactaatcc ccgccaccag
    38341 cgcctcgacc gattccggct ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    38401 ccccagttct gtgtgggctt ctgcggtaat caccgcgtta ccgcctaccg cctaccgcca
    38461 gaatattggt cagtttcgac ggcaatacgg catctgccgc gccgcatttt tgcaccacca
    38521 gatggcaatc gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa
    38581 attgcatgtt gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc
    38641 cctgcccgac aatggcaaaa atcagcggtt catcgcgcag gcgatcggca gcttcaataa
    38701 cgttttccag tccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat
    38761 ccggtaggcc aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac
    38821 gggcgatttc cgaccagttg gggaagaaaa tgatgttatc cgccgccacg cctttttcga
    38881 tggctttatt catcatcgaa cgcgaaatcg tggagacgtt atcgacgtta tgcagtccgc
    38941 tacgttcgaa cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc
    39001 ccagccccag catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag
    39061 agagtttcgc cagcaggcgc attcccggcg tgcaaaacag cgttggcacc acgccgataa
    39121 tgcgatccgg cttccagcga cgttgcgcca tcaacgggaa aaaactgctg acggcaaaac
    39181 cgccgagatg caacaggcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc
    39241 gccacaccgt ggcggccccc tcttctcgtt tgtagcgcta ggcggaatag ttctcgccca
    39301 cctgccactg cgggtagtaa ggcggtgcgg taataacccg cacctcatga ccttgcgccg
    39361 ccagccattc caccatttct ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt
    39421 taatgccgta gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa
    39481 ataggcgcgg ctgttagcgt gaacattgtc actggcgagc agtgcgtccg gcgtcagcca
    39541 gcggtaatcg tcatgctgct catccggcag taacagttct tcttccgcta ctctgaagcg
    39601 aaaaccgagc accacatagt gagtggtgaa atccgtgcca gagaagttat cgtcataaaa
    39661 gtgctgccag acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc
    39721 catcgtcagc cgctcaaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg
    39781 cacaaaccag taaccctgcg ccgggcggtt ggttcttttg ccaggcagaa actcgccgcg
    39841 actgttctcg acaataaagt cgagagagac aagcggtgtg gagcgcacta ccgtggcaaa
    39901 gtcttcctga cgtaaaaaca tcattacccc cgaaagcggt cttgattctc aaggaaccac
    39961 tggtaagtgc tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc
    40021 aggcgcgtca catcaagcag tttgcgcggc gtaccatccg gtttactggc atcaaaaacc
    40081 acccgacctt tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag
    40141 tcaacgcccg tgccgacgtt aatgtgcgac agcatcggct gggtgttttc cagccagact
    40201 tcgtgcgcca gctccatcac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat
    40261 tcacgcatcg gtgtaccgct gccccacacc accacgtccg gcgcattctg tgccgtcgcc
    40321 tcgtggaagc gacgcagcaa tgctgggatc acatgcgaat tactcgggtg gaagttgtca
    40381 tgcgggccat acaggttggt cggcatgacc gagcggtaat ctcgtccgta ctggcgattg
    40441 taagattcgc acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc
    40501 tccagcgtgc tctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag
    40561 atgcaggacg atccgagaaa cagcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg
    40621 atgttgctct caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca
    40681 acaatgccgc ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag
    40741 aaatcatgca cggcgcggct gtccagcagg ttcagctcgt cgcgggtacg taataccagc
    40801 tccacatcac cgcgctgttc gagctgccgc ttgatggcag aaccgaccat cccacgatgg
    40861 ccagcgataa aaatgcgttg tttactcatg cttatgactc cagcgcgatc gccacgtcgt
    40921 agccgtgaga tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt
    40981 cagacaccat ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg
    41041 tcgggtcgtc gagcagcgtt tcaacttctg ccggacggaa gtaacgcgga tcaacggcga
    41101 taatcacatc acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc
    41161 ccttctcttc aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gccgctgcca
    41221 tttccacgaa ctgacgtacg gagtactgaa cgccggtcac gataacgaaa tcttccggct
    41281 gttcctgttg cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac
    41341 gcagggaatc catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga
    41401 ttgcgcgggt gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga
    41461 agagaatacc gttacaagcg tacatgccgt aggattcgca gtagttaacg gtgatccagt
    41521 aggcgtacag tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct
    41581 gcggaatttc ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt
    41641 tcttttccag accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga
    41701 cgtcagcggt atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcccaggt
    41761 tgtacacttc atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca
    41821 ggtcgccata atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat
    41881 ccacgcgctc ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt
    41941 tttccagcag aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag
    42001 cgacttttga catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg
    42061 cgtatcacca ctgcgggatt cccccggcaa accacattta ccggaagcga tttaaaaaca
    42121 ctgcttcgtg cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca
    42181 tcggttgcca gccagcattt ctcgccaatc
//
LOCUS       DAJVOY010000010        41642 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVOY010000010 DAJVOY010000000
VERSION     DAJVOY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.010"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..255
                     /locus_tag="OQL90_000509"
     CDS             <1..255
                     /locus_tag="OQL90_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1367393.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OQL90_000510"
     CDS             complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OQL90_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1367394.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OQL90_000511"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OQL90_000511"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1367395.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="OQL90_000512"
     CDS             complement(2907..3182)
                     /locus_tag="OQL90_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1367396.1"
                     /translation="MSRTIFCIFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OQL90_000513"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OQL90_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1367397.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="OQL90_000514"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="OQL90_000514"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1367398.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="OQL90_000515"
     CDS             5142..5468
                     /locus_tag="OQL90_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1367399.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="OQL90_000516"
     CDS             5652..6371
                     /locus_tag="OQL90_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1367400.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7593
                     /gene="ansB"
                     /locus_tag="OQL90_000517"
     CDS             6547..7593
                     /gene="ansB"
                     /locus_tag="OQL90_000517"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1367401.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7710..8717
                     /locus_tag="OQL90_000518"
     CDS             7710..8717
                     /locus_tag="OQL90_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1367402.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OQL90_000519"
     CDS             complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OQL90_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1367403.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10001..10594)
                     /locus_tag="OQL90_000520"
     CDS             complement(10001..10594)
                     /locus_tag="OQL90_000520"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1367404.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVTQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10602..10892)
                     /locus_tag="OQL90_000521"
     CDS             complement(10602..10892)
                     /locus_tag="OQL90_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1367405.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OQL90_000522"
     CDS             complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OQL90_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1367406.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OQL90_000523"
     CDS             complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OQL90_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1367407.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12195..13175
                     /locus_tag="OQL90_000524"
     CDS             12195..13175
                     /locus_tag="OQL90_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1367408.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OQL90_000525"
     CDS             complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OQL90_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1367409.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13767..14330)
                     /locus_tag="OQL90_000526"
     CDS             complement(13767..14330)
                     /locus_tag="OQL90_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1367410.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTASADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OQL90_000527"
     CDS             complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OQL90_000527"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1367411.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OQL90_000528"
     CDS             complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OQL90_000528"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1367412.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OQL90_000529"
     CDS             complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OQL90_000529"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1367413.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(17015..17512)
                     /locus_tag="OQL90_000530"
     CDS             complement(17015..17512)
                     /locus_tag="OQL90_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1367414.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OQL90_000531"
     CDS             complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OQL90_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1367415.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OQL90_000532"
     CDS             complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OQL90_000532"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1367416.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(20877..21092)
                     /locus_tag="OQL90_000533"
     CDS             complement(20877..21092)
                     /locus_tag="OQL90_000533"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367417.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            21228..21359
                     /gene="yqgB"
                     /locus_tag="OQL90_000534"
     CDS             21228..21359
                     /gene="yqgB"
                     /locus_tag="OQL90_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1367418.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21368..23344
                     /gene="speA"
                     /locus_tag="OQL90_000535"
     CDS             21368..23344
                     /gene="speA"
                     /locus_tag="OQL90_000535"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1367419.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23481..24401
                     /gene="speB"
                     /locus_tag="OQL90_000536"
     CDS             23481..24401
                     /gene="speB"
                     /locus_tag="OQL90_000536"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1367420.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OQL90_000537"
                     /gene_synonym="yggG"
     CDS             complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OQL90_000537"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1367421.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25643..27634
                     /gene="tkt"
                     /locus_tag="OQL90_000538"
     CDS             25643..27634
                     /gene="tkt"
                     /locus_tag="OQL90_000538"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1367422.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            27948..28391
                     /gene="cmtB"
                     /locus_tag="OQL90_000539"
     CDS             27948..28391
                     /gene="cmtB"
                     /locus_tag="OQL90_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCS1367423.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHG"
     gene            28419..29807
                     /gene="cmtA"
                     /locus_tag="OQL90_000540"
     CDS             28419..29807
                     /gene="cmtA"
                     /locus_tag="OQL90_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCS1367424.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29822..31099
                     /locus_tag="OQL90_000541"
     CDS             29822..31099
                     /locus_tag="OQL90_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1367425.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31096..32061
                     /gene="yggF"
                     /locus_tag="OQL90_000542"
     CDS             31096..32061
                     /gene="yggF"
                     /locus_tag="OQL90_000542"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1367426.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            32083..32592
                     /gene="fumE"
                     /locus_tag="OQL90_000543"
     CDS             32083..32592
                     /gene="fumE"
                     /locus_tag="OQL90_000543"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCS1367427.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32589..33302
                     /locus_tag="OQL90_000544"
     CDS             32589..33302
                     /locus_tag="OQL90_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1367428.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33274..33951)
                     /locus_tag="OQL90_000545"
     CDS             complement(33274..33951)
                     /locus_tag="OQL90_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1367429.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(33945..34622)
                     /locus_tag="OQL90_000546"
     CDS             complement(33945..34622)
                     /locus_tag="OQL90_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1367430.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34610..35317)
                     /locus_tag="OQL90_000547"
     CDS             complement(34610..35317)
                     /locus_tag="OQL90_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1367431.1"
                     /translation="MHPFTSLILWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35318..35896)
                     /locus_tag="OQL90_000548"
     CDS             complement(35318..35896)
                     /locus_tag="OQL90_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1367432.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(35919..36350)
                     /locus_tag="OQL90_000549"
     CDS             complement(35919..36350)
                     /locus_tag="OQL90_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1367433.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36722..37741
                     /gene="epd"
                     /locus_tag="OQL90_000550"
     CDS             36722..37741
                     /gene="epd"
                     /locus_tag="OQL90_000550"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1367434.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37791..38954
                     /gene="pgk"
                     /locus_tag="OQL90_000551"
     CDS             37791..38954
                     /gene="pgk"
                     /locus_tag="OQL90_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1367435.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39169..40248
                     /gene="fbaA"
                     /locus_tag="OQL90_000552"
     CDS             39169..40248
                     /gene="fbaA"
                     /locus_tag="OQL90_000552"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1367436.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            40606..41466
                     /gene="mscS"
                     /locus_tag="OQL90_000553"
     CDS             40606..41466
                     /gene="mscS"
                     /locus_tag="OQL90_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1367437.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
BASE COUNT        10193 a        10411 c        11090 g         9948 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaaa tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag atcggtcacc
     4081 gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccagggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaaa caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcaac tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagag ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgca
     7021 gctgataaag catccgctaa tcgtggcgtg ctggtggtga tgaacgacac cgtactggac
     7081 ggtcgcgatg taaccaaaac caacaccacc gacgtagcga ccttcaagtc tgttaactac
     7141 ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc ggcacgtaag
     7201 cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa agtcggcatc
     7261 gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga tgcgggctat
     7321 gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt gttcgacacc
     7381 ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt accgacgggt
     7441 gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg
     7501 ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac caaagatccg
     7561 cagcagatcc agcagatctt caatcagtac taatcacctc gctccggtat cgtgccgggg
     7621 ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga taatcaccgg
     7681 aataaattat ttcgcgtgag gtttttcggg tgaaaaagca atggattgtt ggcacggcac
     7741 tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact gaaaatatct
     7801 taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct atcaccttaa
     7861 aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat gtctcttcca
     7921 gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc gaacagacct
     7981 ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga acgcccgcgt
     8041 ctggctggtc ggtgaacttt tactcttttc agtcggcagc cagcgatcgt gggcgggtgg
     8101 tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat ttcaattatc
     8161 gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct gcgctggaaa
     8221 aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt
     8281 ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa aaaattcacc
     8341 aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt aaatatgaca
     8401 aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag tgttatgaag
     8461 tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag acttttctgc
     8521 aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa aaagggcaat
     8581 atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa
     8641 ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa ctgcggcgca
     8701 atggggtgat taagtaagtt tgatatagtg cacgatttgt agcaggatgc ggcgttgacc
     8761 ccgcatccgg caatcaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgt
     8821 tgccacatgt aggctggata aggcgttcac gccgcatccg gcaatacaag tttactcagc
     8881 cagaaaaagc tccagcagcg aatttaaaaa cagcttccca tgttccgtta tctgccagta
     8941 atccgcacat tctgtgagat aaccctgggc aatcgcctcg tctaactgtg ggcgaatcac
     9001 atcttcgcaa agcccggtat acgcactaaa ctctgcgcgt ggcgcggcct ccagcaagcg
     9061 aaagcggttc ataaagaact caaacggctt atctgcggct tcgacatcac gctggctttc
     9121 cagatacctt ccttgcataa aaccacgagg atgacgcgtt ttagtggtac gcagaatgcg
     9181 cccatccggg aaggtgactt tgccgtgcgc gccgcagcca ataccgatgt agtcaccaaa
     9241 tcgccagtag ttgagattgt gctggcactg ataaccgggt ttggcgtaag cggaagtttc
     9301 atattgctga taacccgctg cggttaataa ctgatgcccc tgttcgaaaa tatcccacag
     9361 cgcgtcatcg tccggcagca ccggtggtcg cgaaccaaac agcgtattgg gttcgatggt
     9421 cagttgatac caggaaagat gcggcggatt cagttcaatg gcctggcgta gatcgccaag
     9481 cgcctcttcc agtgattgat ccggtagtcc atgcatcaaa tcaaggttaa agctacgtaa
     9541 ccctaaaccg ctcgccagct ttgccgctcg tttcgcttct tgcgggccat gaatgcgccc
     9601 aagtcgtttc agcttttctt cgctaaaact ctgcacacca atagagatgc ggttcacacc
     9661 agcacgctga taatcgacaa agcgatcggc ttctaccgta ccagggttcg cttccatagt
     9721 aatttctgca tccgctgcca gcggcaaacg cgcacgcacg ccgtccagca gcgtttgcat
     9781 cgccgggccg gaaagcaggc tcggcgtacc accgccaata aagattgtct ttacttcacg
     9841 gccctgagcg taagccacat cattgtccag atcgcaaagc agatgctgaa cataatcgtc
     9901 gtgcggcact tctcctttca acgcgtgaga gttgaaatcg cagtacgggc atttctgcac
     9961 gcaccacggg atgtgaatgt agagactcag cggcggtaat ttaaccatta cgtaaagcgt
    10021 ccagcagcag tttcaacgcc tgaccacggt gggaaatggc gctcttttct tcgcgggtca
    10081 gttcggcggc ggttttccct tcggaaggta cgaagaagat tggatcataa ccaaagccac
    10141 cagtgcccgc tggttcgcga gtaatcacgc ccggccagct gccgtggcat accagcggag
    10201 tcggatcttc cgcgtgacgc agatatacca gcacgcagtg gaaacgtgcc tgacgttggt
    10261 cgtccggtac gtctttcagt gtttccaaca gtttttgcag attcttttga tcggtcgcgt
    10321 cttcaccgga ataacgcgcg gagtaaatac ccggcgcgcc acccagcgca tctaccgcca
    10381 gaccagagtc gtcggcaatt gccggtaaac cggtcacttt tgccgcatgg cgcgctttca
    10441 gaatcgcgtt ttcgataaag gttaggccgg tttcttcagc ggaatcaacg ccgaggtctg
    10501 tttgtgtcac gatatcaaga ccaaagtcgc taagcagcga cgccagctca cgcactttac
    10561 cggcattgcc ggttgcgagg acaacttttt gcataggata cctaattaat taacgccgcg
    10621 atttctggcg ggatttgttg cggattaatg attttaattt gtttgtggcg gccaagttcg
    10681 cctttttcaa tcaccacctg gcttttggca acccggaatt gcttaccgag aaacttcacc
    10741 agatgactgt tggcctggcc gtcaactggc ggcgcggtaa tggcgacttt aacttcgtcg
    10801 ccatgtaaac cgacaataga atcacggctg gctttcggct gaatatagag ccgtaaaacc
    10861 agaccgtcat cattaactgt tacggcattc ataacgccat ccacatcccc ggcagcagga
    10921 tgtttccggt tgcctgtaat acttctgcga cacccatatt gatgacatac agcagcagaa
    10981 cgaggatcat cggcgagaaa tcaatcccac ccattgccgg tagcaagcgg cgaatcgggc
    11041 gcagcagcgg atcggccagc tgaatcagca cgtattcaat cgggctacga ccctggctta
    11101 cccagctcat aatcgccatc accagcagga cccagaaaat cagcaagccg atggttttca
    11161 gcagaatcag taaaccggca atccagatga ttggcaggaa ggtcaccact ttaaacagca
    11221 caatggcttt gataaaactg agaatatagg caaccagcag cgaggcgctg tcaatgggac
    11281 ccattgccgg aataacgcgg cgcagtggcc cgataattgg ctgcgttacc ttcactacaa
    11341 actgtgagaa ggggttgtaa aaatcacaat gagcccactg catccagatg cgtaataaca
    11401 gcaccatggt atacagctca atgaccgttg aaagcaggaa agtcaacgta ttcatggcgt
    11461 tcctttaatt ccttattttt tagagtaatc acgcgcacca aaaattgcag tgccgatacg
    11521 aaccatcgtg ctacctgccg caatggcggc ttccatatcg tccgacattc ccagagagag
    11581 cgtgtcgata tgcgggtagc gcgttttcag tccggcaaat gctacagcca tttggcgtgc
    11641 aacttcaaac tgccttacat attctgactc aggcgcaggg attgccatca gcccgcgcag
    11701 acgtaaacgc ggtagttcag cgaccgccgc tgccagctcg tccagttctg ccagttgaat
    11761 cccggactta ctgttttcat cactaatatt aatttgaatc agtacgttaa gagggggaag
    11821 ttctgccggg cgctgatcgt tgaggcgggt agcaatgcgc aaacggtcga tggtatgaca
    11881 ccagtcgaaa tgctctgcca ccaggcggct tttattagac tgcaacgggc caataaaatg
    11941 ccattctaat cctgttacgc ccagttcctg aaagtggcga attttatcta ccccttcctg
    12001 aacgtagttt tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga tggcgctcgc
    12061 aggttttgtt ttactgactg caagcagcgt aatttcttct ggagaacggc cgcaacgcgt
    12121 tgcagccgct gagattttgt cccggacctg tgccaggtta tgcgcaatat cgttcatttt
    12181 ccgaggatct tagtatgaat atggaagaaa ttgtggccct tagtgtaaag cataacgtct
    12241 cggatctaca cctgtgcagc gcctggcccg cacgatggcg cattcgcggg ctaatggaag
    12301 ctgcgccgtt tgatgcgctg gacgtcgaag agctactgcg ggagtggctg gatgacgatc
    12361 agcgggcaat attgctggaa aatggccagc tggattttgc cgtgtcgctg gcggaaaacc
    12421 agcgattgcg tggcagcgca ttcgcgcaac ggcatggcat ttcgctggcg ttacggctgt
    12481 taccttcgca ctgcccgcag ctcgaacagc ttggcgcacc aacggtattg ccggaattac
    12541 tcaagagcga gaatggcctg attctggtga cgggggcgac ggggagcggc aaatctacca
    12601 cgctggcggc gatggttggc tatctcaatc aacatgccga tgcgcatatt ctgacgctgg
    12661 aagatcctgt tgaatatatc tatgccagcc agcgatgttt gatccagcag cgggaaattg
    12721 gtttgcactg tatgacgttc gcatcgggat tgcgggccgc attgcgggaa gatcctgatg
    12781 tgattttgct cggagagctg cgtgacagcg agacaatccg tctggcactg acggcggcag
    12841 aaaccgggca tttggtgcta gcaacattac atacgcgtgg tgccgcgcag gcagttgagc
    12901 gactggtgga ttcatttccg gcgcaggaaa aagatcccgt acgtaatcaa ctggcaggta
    12961 gtttacgggc cgtgttgtca caaaagctag aagtggataa acaggaagga cgcgtggcgc
    13021 tgtttgaatt attgattaac actcccgcgg tggggaattt gattcgcgaa gggaaaaccc
    13081 accagttacc gcatgttatt caaaccgggc agcaggtggg gatgataacg tttcagcaga
    13141 gttatcagca gcgggtgggg gaagggcgtt tgtgacgttg ccatgcccga tgcgcaagtt
    13201 tatagggcct ataaaggcaa taaatgtctg atgcgacgct aacgcgtctt atcagaccta
    13261 cagccgctgc cagatgtgtg ctggataagg cgttcacgcc gcatccggca agatggcggt
    13321 aacgactatc cgccaggctt ttagatcgcc ttaatatccc tgctcgaaat agctttcgag
    13381 aataataacc gcagaggcag agtcgacttt gcctttgttg agcgcccgat agccgccctg
    13441 ttcaaacaga ccggaacggg cttccacggt gctaagacgt tcgtcatgga gctttacttc
    13501 aacaccgaaa cgaccatgaa tacggttggc aaatttacgc gctctggcag tcaatggttg
    13561 ctcggtgccg tccatattca gcggcaaacc gacgatgatt tcgtccggct gccactcttt
    13621 cagtaaacgt tcgatgaggt tccagtccgg cgtaccgtcc tgtgctttaa ttgcaggcaa
    13681 agggcgagca gtgccggtaa tgcgttggcc gaccgctacg ccaatgcttt tggtgccgaa
    13741 gtcgaaggcg agtaaggttc cactcatcag gcgtgccctg ccacaccagg catggtgaga
    13801 atatccacac caatcagttt tgccgcctcg cgccagcggt cggcaatcgg cgttttgaac
    13861 agaatattca gatccgccga ggccgttagc cacgcgttat cgagaatttc ttgttccagt
    13921 tgaccttttt cccaggaggc atagcccaga gccaccaata cgtcagacgg ttgtttatcg
    13981 gtgccgagcg tttccagcac atcgcgggaa gtggtcatca ccgtgttgtc tgaaatgcga
    14041 atgctggaag caaaattgga gggcggagta tgcaaaataa acccgcgatc ttcagccagt
    14101 gggccgccga gcataacagg tttatccaga cggattgatt catcacgcgg ctccggcgtg
    14161 atcttcagct tttccagaat cccttcaatt ttgagatttt ccagcggctt gttgacgatg
    14221 atccccattg caccattggt attatgttcg caaatgtaga ccacggaacg acggaaaatc
    14281 ggatcctgga gagcaggcat ggcaataaga aagtgatgct gtaaattcat tgtcagaggt
    14341 tctgtttcct ggttcaaaaa agcaacagcg cccagtatgc gtggaaagaa gggcgctgtc
    14401 actcagagtc tcaacgagat ccttctcgct aaggtgggtt actgctgctg taaacgtgct
    14461 tcgatggcat ccattaacat tccggtgatc gacaccggaa actctgcttc aatctcacga
    14521 atacaggttg ggctggtgac gttaatttca gtcagacggt cgccgatgat atccagacca
    14581 acaaaaatca gccctttttc tttcagcgtc ggcccgatct gacgggcgat tttccagtca
    14641 ctttccgtca gcggccgagg ttcaccgcga ccaccggcag ccagattgcc acgggtttcg
    14701 cccccctgcg gaatacgcgc caggcagtac ggtaccggct cgccatccac caccagcacg
    14761 cgtttgtcgc catctttaat ggctggcagg taattttgcg ccatgcagta gcgagtgcca
    14821 tgctcagtca gggtttcggc aatcacgccg aggtttggat cgccttcttt cacgcggaaa
    14881 atcgacgcgc cgcccatacc gtccagcggc ttaagaatga tgtcgctgtg tttctcccag
    14941 aacgctttta gctgcgcttt attgcgcgta accagcgttt ctggcgttaa gtcagagaac
    15001 caggcggtaa acagtttctc gttacagtcg cgcaggctct gcggcttgtt aacgatcagc
    15061 gtccctttct cttcggcacg ttccagaata taggtcgcgt agataaactc ggtatcaaac
    15121 ggcgggtctt tacgcatcag gagcacatcg agatcggcca gcggcagatc ctgttcaccg
    15181 acgaacgaaa accactcttc gtagttctgc ttcacgttca gcgtgcgggt atgggcgcga
    15241 gcttcgccat tgatcagata cagatcgccc atctccatat agtgaagttc gtaaccacga
    15301 cgctgtgctt ccagcaacat agcgaaactg gaatctttct tgatgttgat gtttgcgatg
    15361 gggtccatca cgatgccgag cttgatcatt attcttctcc gttagcccaa atcgccaaat
    15421 cgtacttgta gcgcggtaat ggcggtgagc gcagttgtct ctgtacgcaa aacgcgaggt
    15481 cccaacagga tatcagtaaa ttgatagcgg gcagtcatgg caatttcatc tgccgataaa
    15541 ccgccttccg ggccaatcag caggcggacg cgttcaaccg gtaacggcaa cgtattgatg
    15601 ctgttactgg cgcgcgggtg aagattcagt ttcagtcctt catcctgctc tgcacaccag
    15661 gcttccagat ccatcgctgg acggatttcc ggcacccggt tacgaccaca ctgctcacag
    15721 gcagcaattg caatcttctg ccactgctgg agcttcttgt tcagacgttc actatccagt
    15781 ttaacgccgc agcgctcaga aaaaagtggc gtaatgaggc ttacaccgag ttcgatcgat
    15841 ttctggatcg taaattccat tttttcacca cgcgacatca cctgaccgag gtgaatatgc
    15901 agcggagatt cgcgatcgtc gatcttgcct tccagcacct tcacttccac gctttttttg
    15961 ctggcgctgg taatttcggc gtcaaagacc tggttgctac cgtcaaacaa ttgcaacgcc
    16021 tgccccggcc ccatgcgcag tacgcgcccg atatggttgg cggcatcttc gcaaagcgcg
    16081 atgtgagaat ggctggtcag tggttcagga tgataaatgc gggggatacg catagttaaa
    16141 aatccgcgtc gtctccccac gcggttgtac gcgtggggta ggggttaaca aaaagagtcc
    16201 cgctagtgta ggttagctct ttcgcgcctg gcaagcgcgt tgcacatacg ggttatgatt
    16261 gccttgcacc ttcgcgatgc gttcatcgcg ctcgcactcc cagtcggtaa ccggatacat
    16321 cttgttccat gcgttgaaca gctgcgtttg ctggcgagag agtgtcaggt tgtattggtc
    16381 gcgcatatag aagtaggtgc gcgcaatggc accgcgtgca cgcgctggtg gttcggcagc
    16441 tttttctttg aaatcgacct tcatggcgca ttgaccgtac tggccttcgc cgccattcca
    16501 ctggctgtac ataaagttgc cgcgatcgcc attcacctca ccgactgacg gctgcaggtt
    16561 atgcatatcg ctttccatct tgcgatagac cggatcttta gcgcagtttt tacgtccacc
    16621 gtcctgccag cactggcgct ggtgaccgaa ctgccaggcg ggaacgacgt gttcccactc
    16681 tacgcggctg gctcggtttt catttttgcg cacctgatag ccgcacgatt gcagatcaac
    16741 aacgcctttt ttgccctgcc agttaatttt acatccgcaa taaaacgtac cgggcgcgtc
    16801 agcgtggact tttaccgccg cggctttcgc ctgagaaaaa ctattgatac cttcggccaa
    16861 cgccgggccg gaaaatgctg cgctcagtac caccgcagca atagacaaat aacggtacat
    16921 cacttactcc gtgttaaaac gagccagcaa cgtagcgaaa gttgttttgg tatgcaatca
    16981 gccagatcag gaaagttcct gatagttcag atggttattt tgcgaccagt tgttcgccgc
    17041 agtgaacaca gcgatagacg gcttcgccac gaactacgcg attatggcgg cgtacggtaa
    17101 gctgatgctc ctggcacttg cagcggtagg ggaaggtgtt gcgacgcacg gattgcagtt
    17161 cgaactgatg cgtacgacgg gcgggaacac ccagcacgtt ttccatcatc cacttccact
    17221 ctttgccatg tggtgctacg cgcccgaaat gtttccatac cagcaaatgt gccagttcgt
    17281 gcggcaccac ttcttcaata aaggcttcac tgttttccaa cagcaaaacg ggattgaggc
    17341 ggatttcata gctttccagc caggctgttc cggcggaggt gccgcgctgg gtataagaga
    17401 gttttggctc cgggtagtta cgccccaatt tcaggttagc ctgggcgagt ttttcccgca
    17461 ggcgacgcat aacggcctgt tggatggcga tagggagacg ggatgttttc atagcggcag
    17521 aggatagagc gaagaaaaga tgactgcaag aggtggcttc ctccgcgatg ggaggaagct
    17581 tggggagatt aatcgtgagc gcctatttcg cgcagtttac gacctttcat cagattacgt
    17641 tcaatatgtt ccagcgaaac gtgtttggtt tccggtacca gccacaatgt cagcaggata
    17701 aacagtacgt tcagagccgc atacacccag aaggtgttgg cgttacccag tgtgttgagc
    17761 atggtcagga acgttgcgcc aacgatcatg ttggcaatcc agttggtggc ggtagagcag
    17821 gtgatgccaa aatcgcggcc tttcagcggc tgaatttcgg agcacagtac ccaaatcagc
    17881 ggaccggcac tcatggcaaa accgacaata aacatcagca gcatggcgat ggcgaaatac
    17941 tgcgctgacg gagagtgaat accgatatgc atcattgtac cgagtacgcc catgccagca
    18001 gccatcacca ggaagcccag cgttagcgtt ggtttacgtc cccagcggtc aacaaggccg
    18061 attgcgataa aggtggcaag tacgttggtc aggccgacaa tcactgtccc ccacatttgc
    18121 tcggtagtgt tggtataacc cgccagttcg aagattttcg gcgcgtaata catgatgacg
    18181 ttcatcccgg tgaattgctg cattacctgt aacagtacgc caaggaacac cgcgcggcgg
    18241 aagttgctat tctctttaaa cagcgcccag ccactctgtt taacctgcaa actttcacgg
    18301 atttcatcca gttcgcgttt cgcttccgcg ctggtgtcac gcaggcgtag cagcacgcgt
    18361 tcggcatcaa caaaacggcg tttggcggca aaccaacgtg ggctgtctgg caggaagaag
    18421 acaccaatca gcagcaaaat tgccgggatg ataatcacac ccagcatcca gcgccatgca
    18481 ccggtgtagc tgaaggcggt atcagaaaga taagcaccga ggatcccgat agtgatcatc
    18541 aactgataca tcgagatcat actgccacga attttttccg gcgcaatttc agagaggtac
    18601 agcggtgcgg tataagaggc cacacccacc gccagcccca gtagaacgcg ggaaagaatc
    18661 agtacttcaa cgtttggcgc agccgcagag aacagcgaac cggcaacaaa caaaattgcg
    18721 ccgatcatca ggctcttttt gcgcccgagt ttaaaggaga gccagccgct gcccaccgca
    18781 ccgactgccg caccgaacat catggagctt acgacccatt cttgcgtgtg cgaagtaatc
    18841 tggaattcat ctgcaataaa cggcagtgcg ccagcaatta caccgatatc caggccaaag
    18901 agtaatcccg ccagagcggc aaggaagcag acgaaaaacg tcattgcctt gtttgaccgc
    18961 ccctgttttt tagcgtcagg catgatgccc tccaatatgg ttatttttta ttgtgaatta
    19021 agataggtga gtacgacgta aaaagatgtg aagcaaatca catcagtgta atcgcttaca
    19081 ctaacattta tcttagttat ggtttaagtg catgaaaata attgagttat atcatcatag
    19141 attaaacgct gttatctgca attaagactt tactgaaaag aaatgtaaca actgtgaaaa
    19201 ctgaataata atatgaaaat attacaaata tttggctggt gttattggtg taatcggttt
    19261 cagtagtgat gataatttgc gataaaacgt gttgaggaag aatgatgtta ttgtgcgggc
    19321 agctttttgc cggatgcggc gtgaatgcct atccaccgcg gtgaggtaaa agaaaaaggc
    19381 cagcgcgagg ctggcctttg aaagcaggtg aagaaagatt acttcagacc ggcagcatcg
    19441 cgcagcagct gcgctttgtc ggttttttcc cacgggaaat gttcacgacc aaagtgaccg
    19501 tatgctgcgg tttctttgta gatcgggtgc agcagatcca gcatctgaat cagaccgtat
    19561 gggcgcaggt cgaagaactc acgtaccagc agagtcagtt gttcagaagg cactttctca
    19621 gtaccgaaag tttctaccat gatggaggtc ggttcagcca cgccgattgc gtaggaaacc
    19681 tgaatttcac aacgatcggc caggccagca gcaacgatgt ttttcgcgac ataacgtgct
    19741 gcgtaggctg cggaacggtc cacttttgat ggatctttac cagagaatgc tccgccaccg
    19801 tgacgtgcca tgccgccgta ggtatcaacg ataattttac gaccagtcag accgcagtca
    19861 cccatcgggc caccgataac aaaacgaccg gtcgggttga tgaagaatgt ggtggcagaa
    19921 gtcagccatt cagcgggcag aattggcttg atgatctctt ccattaccgc ttcttgcagc
    19981 gatttctggt cgatctcttc agagtgctga gtggaaagca cgacagcatc aataccaacg
    20041 attttgccgt cgtcatactg gaaagtcacc tggcttttcg cgtccgggcg cagccacggc
    20101 agagtgccgt ttttacgcac ttcagcctga cgctgtacca gacggtgtgc ataggtgata
    20161 ggtgctggca tcagcacgtc ggtttcatta gttgcgtagc caaacatcag accctggtca
    20221 cccgcgccct gttccagcgg atcggcacgg tcaacgccct ggttgatgtc aggagactgt
    20281 ttgccgatag cgctcagaac cgcacaggag ttagcgtcaa agcccatgtc ggaatgcaca
    20341 tagccaattt cgcgaacggt gttacgggtg atctcttcga tgtctaccca ggcgctggtg
    20401 gtgatttcgc cgccaactaa aaccatgccg gtttttacgt aggtttcgca agcaacgcgt
    20461 gctttcggat cctgttcgag gatcgcgtct aaaacggcat cagagatttg gtcagcaatt
    20521 ttgtcaggat gcccttcaga gacggactcg gacgtaaaaa ggtgttttgc catatttaat
    20581 atcacctaaa gagaatttgg ttagctcaaa ctgttgtgtg gattttctgt ggtagcggat
    20641 cctaccacga ctctgcaggt taaaaacact ggcagtctga gtgttaatcg gtatggatgg
    20701 attaacatct ggatggctat tttaggtcaa ttcttcaccc gatttccact tttttttgaa
    20761 tcgtgtctca ttctgttaaa aacgtggctg gaaatttttt ctgacaatgc cggcattctg
    20821 cgtatttata ttttgcaatt ttctgccatt gtggggtata aaacgcggcg cgcggcttaa
    20881 ataaaaagca cacgacgttt ctttcgtgtt gccacttcca gccgggttga aatcagagtt
    20941 ttggcttgtg ggttcgtctt aacaggcggc cgtggaggtg atacgaaata atgaaccgta
    21001 gtctgctgct taacctgtct caccgttctg gtgaagattc gttccccgca ctctgcatct
    21061 ctgctttgca tacctgccga tgttataccc atctcggcgc ttctcaggat tcaagagctg
    21121 gttacagtta ctgaggactg aacaagggcg ctcttgtaaa aacaagagtt ttctcgtggt
    21181 ttcgccgaac tgtcacactt acgttcggtt atgtgcttaa taatgttatg aaaaagaaac
    21241 cggttgcgca gttggagcgt cagcattcac tgctggaaaa tccatgtgct tatgggttgt
    21301 tatcgcagtt ccaggctgcg atagtcgtta actgttttac acttaataaa ataatttgag
    21361 gttcgctatg tctgacgaca tgtctatggg tttgccttcg tcagcgggcg aacacggtgt
    21421 actacgctcc atgcaggagg ttgcaatgag ctcccaggaa gccagcaaga tgctgcgtac
    21481 ttacaatatt gcctggtggg gcaataacta ctatgacgtt aacgagctgg gccacatcag
    21541 cgtgtgcccg gacccggacg tcccggaagc tcgcgtcgat ctcgcgcagt tagtgaaaac
    21601 tcgtgaagca cagggtcagc gtctgcctgc actgttctgt ttcccacaga tcctgcagca
    21661 ccgtttgcgt tccattaacg ccgcgttcaa acgtgcgagg gaatcctacg gctataacgg
    21721 cgattacttc cttgtttatc cgatcaaagt taaccagcac cgccgcgtga ttgagtccct
    21781 gattcattcg ggcgaaccgc tgggtctgga agccggttcc aaagccgagt tgatggcagt
    21841 gctggcacat gctggcatga cccgtagcgt catcgtctgc aacggttata aagaccgcga
    21901 atatatccgc ctggcattaa ttggcgagaa gatggggcac aaggtctatc tggtcattga
    21961 gaagatgtca gaaatcgcca ttgtgctgga tgaagcagaa cgtctgaatg tcgttcctcg
    22021 tctgggcgtg cgtgcacgtc tggcttcgca gggctcgggt aaatggcagt cttccggcgg
    22081 ggaaaaatcg aagttcggcc tggctgcgac tcaggtactg caactggttg aaacgctgcg
    22141 tgaagctggg cgtctcgaca gcctgcaact actgcacttc cacctcggtt cgcagatggc
    22201 gaatattcgc gatatcgcca cgggcgttcg tgaatccgcg cgtttctatg tggaactgca
    22261 caagctgggc gtcaatattc agtgcttcga cgtcggcggc ggtctgggcg tggactatga
    22321 aggcacccgt tcgcagtctg actgctccgt aaactacggc ctcaatgaat atgccaacaa
    22381 catcatctgg gcgattggtg atgcgtgtga agaaaacggt ctgccgcatc cgacggtaat
    22441 caccgaatcg ggtcgtgcgg tgactgcgca tcacaccgtg ctggtgtcta atatcatcgg
    22501 cgtggaacgt aacgaataca cggtgccgac cgcgcctgca gaagatgcgc cgcgcgcgct
    22561 gcaaagcatg tgggaaacct ggcaggagat gcacgaaccg ggaactcgcc gttctctgcg
    22621 tgaatggtta cacgacagtc agatggatct gcacgacatt catatcggct actcttccgg
    22681 cacctttagc ctgcaagaac gtgcatgggc agaacaactc tatctgagca tgtgccatga
    22741 agtgcagaag caactggatc cgcaaaaccg tgcgcatcgt ccgataatcg acgaactgca
    22801 ggaacgtatg gcggacaaaa tgtacgtcaa tttctcgctg ttccagtcaa tgccggacgc
    22861 atgggggatc gaccagttgt tcccggttct gccgctggaa gggctggatc aagtgccgga
    22921 acgtcgcgct gtgctgctgg atattacctg tgactctgac ggtgctatcg accactatat
    22981 tgatggtgat ggtattgcca cgacaatgcc aatgccggag tacgatccag agaatccgcc
    23041 gatgctcggt ttctttatgg tcggcgcata tcaggagatc ctcggcaaca tgcacaacct
    23101 gttcggtgat accgaagcgg ttgacgtgtt cgtcttccct gacggtagtg tagaagtgga
    23161 actgtctgac gaaggcgata ccgttgcgga tatgctgcaa tatgtgcagc tcgatccgaa
    23221 aacgctgtta actcagttcc gcgatcaagt gaagaaaacc gatcttgatg ctgaactgca
    23281 acaacagttc cttgaagagt tcgaggcagg tttgtacggt tatacttatc tggaagatga
    23341 gtaagtccgt attacttgaa tccgcttaat ttagcggtga taatccgcca caatttattg
    23401 tgacaaatcc aacccttcct cgtcgggcct aacgacgcgg aagggttttt ttatatcgac
    23461 tttgtaatag gagtccatcc atgagcacct taggtcatca atacgataac tcactggttt
    23521 ccaacgcctt tggtttttta cgcctgccga tgaacttcca gccgtatgac agcgatgctg
    23581 actgggtgat tactggtgtg ccgttcgata tggccacttc tggtcgtgcg gggggacgcc
    23641 acggtccggc agcgatccgt caggtttcga cgaatcttgc ctgggaacac aatcgcttcc
    23701 cgtggaattt cgacatgcgt gagcgcctga acgtcgtgga ctgcggcgat ctggtatatg
    23761 cctttggcga tgcccgtgag atgagcgaaa aactgcaggc acacgccgag aagctgctgg
    23821 ctgccggtaa gcgtatgctc tctttcggtg gtgaccactt tgttacgctg ccgctgctgc
    23881 gtgctcatgc gaagcatttc ggcaaaatgg cgctggtaca ctttgacgcc cacaccgata
    23941 cctacgcgaa cggttgtgaa tttgaccacg gcaccatgtt ctataccgcg ccgaaagaag
    24001 gtctgatcga cccgaatcat tccgtgcaga ttggtattcg taccgagttt gataaagaca
    24061 acggttttac cgtgctggac gcctgccagg tgaacgatcg cagcgtggat gacgttatcg
    24121 cccaagtgaa acagattgtg ggtgatatgc cggtttacct gacctttgat atcgactgcc
    24181 tagatcctgc tttcgcgccg ggaaccggta cgccagtgat tggcggcctg acctccgatc
    24241 gcgccattaa actggtacgc ggcctgaaag atctcaacat tgttgggatg gacgtagtgg
    24301 aagtggctcc ggcatacgat cagtcggaaa tcaccgctct ggctgcggcg acgctggcgc
    24361 tggagatgct gtatattcag gcggcgaaaa agggcgagta agcaccagat gcgatgcgca
    24421 cgggtaaaac gtgccattaa tgtcggatgc ggcgtgaacg ccttatccga cctacgttcg
    24481 gcacccgtag gccggataag atgcgccagc atcgcatccg gcaatgcgca caaggtaaca
    24541 aatgtgccat taatgtcaga tgcggcgtga acgccttatc tgacctacgt tcgacaccac
    24601 caggctttac ttaatcccat ccgcgctcat gcgatcgcga atatgctggg cgcgttcggc
    24661 ggatgccgga tggtcgtcaa acattgagct ttggcggcct tcttccagtt tcgccagttt
    24721 ttcaaagctg gtggcaagac ctgccgggct gatgccgcgt tggcgcagaa gatcgtaaga
    24781 ataatcatcg gcttctgctt cctggcgctg ggagaattgc gaattgacta atttctcgcc
    24841 cagatcacca agttgtgatt gagataaact gccgacaatc ccgcccgcag aggccgcagc
    24901 tactcgcacg gcatttgtac caagcgccac ctgcatccct ttcttcacat ggcctaacgc
    24961 cacgtgcccc atctcgtgac cgatcaccgc ttcgacttcg ttatccgtca tcatatccat
    25021 cagcccgcta tagacgcgga tacagccgtt agccattgca aaggcgttca catccttcgc
    25081 catatacact ttgtaattca ccggctgacc gttgatattg ttgcctagcg cattggcaat
    25141 agttgtcaga cgtttagcgt attcgctatt ggctggcgca atcgtcgcct tgctgtccat
    25201 ctcctgacat gcctgatcgc tcagggtttt cacctgcgca tcactcaaac tgtaagcctg
    25261 aaaagcttcc gctcctgatg agagcagtcc gttggagtcc atattctggc aaccagtcag
    25321 taccgttgcc acgctcattg ctaccaataa ggcgcgaatt ttcatgtttt ctcttccgca
    25381 caaggtcagg attattctaa aaatgcgccg tttgcaggtg aatcgacgct cagtctcagt
    25441 ataaggaaaa gcgcagcgga cgggcgagta gattgcgcaa catgcgagca tgatccagag
    25501 atttctgaag cggcaaaagg atgttccatg tacatgacgc gcggcttgcg gtaaattgtt
    25561 ggcaaatttt ccggcgtagc ccaaaacgcg ctgtcgtcaa gtcgttaagg gcgtgccctt
    25621 catcatccga tctggagtca aaatgtcctc acgtaaagag cttgccaatg ctattcgtgc
    25681 gctgagcatg gacgcagtac agaaagccaa atccggtcac ccgggtgccc ctatgggtat
    25741 ggctgacatt gccgaagtcc tgtggcgtga tttcctgaaa cacaacccgc agaatccgtc
    25801 ctgggctgac cgtgaccgct tcgtgctgtc caacggccac ggctccatgc tgatctacag
    25861 cctgctgcac ctcaccggtt acgatctgcc gatggaagaa ctgaaaaact tccgtcagct
    25921 gcactctaaa actccgggcc acccggaagt gggttacacc gctggtgtgg aaaccaccac
    25981 cggtccgctg ggtcagggta ttgccaacgc agtcggtatg gcgattgcag aaaaaacgct
    26041 ggcggcgcag tttaaccgtc cgggccacga cattgtcgac cactacacct acgccttcat
    26101 gggcgacggc tgcatgatgg aaggcatctc ccacgaagtt tgctctctgg cgggtacgct
    26161 gaagctgggt aaactgattg cgttctacga tgacaacggt atctctattg atggtcacgt
    26221 tgaaggctgg ttcaccgacg acaccgcaat gcgtttcgaa gcttacggct ggcacgttat
    26281 tcgcgacatc gacggtcatg acgcggcatc catcaaacgc gcagtagaag aagcgcgcgc
    26341 agtgactgac aaaccgtccc tgctgatgtg caaaaccatc atcggtttcg gttccccgaa
    26401 caaagccggt actcacgact cccacggtgc gccgctgggc gacgctgaaa ttgccctgac
    26461 ccgcgaacag ctgggctgga aatatgcacc gttcgaaatc ccgtctgaaa tctatgctca
    26521 gtgggatgcg aaagaagcag gccaggcgaa agaatccgca tggaacgaga aattcgctgc
    26581 ttacgcgaaa gcttatccgc aggaagccgc tgaatttacc cgccgtatga aaggcgaaat
    26641 gccgtctgac ttcgacgcga aagcaaaaga gttcattgct aaactgcagg ctaacccggc
    26701 gaaaatcgcc agccgtaaag cgtctcagaa tgctatcgaa gctttcggtc cgctgttgcc
    26761 ggaattcctc ggcggttctg ctgacctggc accgtctaac ctgaccctgt ggtctggttc
    26821 taaagcaatc aacgaagatg ctgcgggtaa ctacatccac tacggtgttc gcgagttcgg
    26881 tatgaccgcg attgctaacg gtatctccct gcacggtggt ttcctgccgt acacctccac
    26941 cttcctgatg ttcgtggaat acgcacgtaa cgccgtacgt atggctgcgc tgatgaaaca
    27001 gcgtcaggtg atggtttaca cccacgactc catcggtctg ggcgaagatg gcccgactca
    27061 ccagccggta gagcaggtcg cttctctgcg cgtaaccccg aacatgtcta catggcgtcc
    27121 gtgtgaccag gttgaatccg cggtcgcgtg gaaatacggt gttgagcgtc aggacggccc
    27181 gaccgcactg atcctctccc gtcagaacct ggcgcagcag gaacgaactg aagagcaact
    27241 ggcaaacatc gcgcgcggtg gttatgtgct gaaagactgc gccggtcagc cggaactgat
    27301 tttcatcgct accggttcag aagttgaact ggctgttgct gcctacgaaa aactgactgc
    27361 cgaaggcgtg aaagcgcgcg tggtgtccat gccgtctacc gacgcatttg acaagcagga
    27421 tgctgcttac cgtgaatccg tactgccgaa agcggttact gcacgcgttg cggtagaagc
    27481 gggtattgct gactactggt acaagtatgt tggcctgaat ggcgctatca tcggtatgac
    27541 caccttcggt gagtcagctc cggcagagtt gctattcgaa gagttcggct tcaccgtcga
    27601 caacgtcgtt gcgaaagcaa aagaactgct gtaattagca tttcgggtaa aaaggtcgct
    27661 tcggcgacct tttttattac cttgatatgt ccgtttgcgg acaagcaata gataaagcgt
    27721 gttgcagatc acaaatattt atatgcaata aatatcaatt atgtaatatg catcacgata
    27781 tgcgtattga catttgttgt tatatctata actcaatgtt atataagaaa ttaataattc
    27841 actgttttca aaacaccggt ttccctgctc aattgctttc attaaaccgc acgagtattt
    27901 tatttttacc gccggacggt accctaaaaa taaaataagg ataatttatg cggcttagtg
    27961 actattttcc agaatcatca atctcagtta tacattcagc aaaagattgg caggaagcta
    28021 tcgatttctc gatggtatca ttgctggata aaaactatat cagcgagaat tacattcagg
    28081 caattaaaga ttccaccatt aacaatggcc cttattatat tctcgcacca ggcgtggcaa
    28141 tgcctcatgc gcgaccggaa tgtggggcgc ttaaaaccgg gatgtctttg acattacttg
    28201 aacaaggtgt ttattttccg gggaatgacg aaccaattaa attactcatc ggactctctg
    28261 ctgccgatgc cgattcgcac attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag
    28321 aagaaatact cgaacaactc ttaacagcat catcagaaaa acaattagcg gacattatca
    28381 gccacggata attactctct cctctacagg ataatattat ggaaaacaag tctgctcgtg
    28441 caaaggtcca ggcttttggg ggctttttga ctgcaatggt catccccaat attggtgctt
    28501 ttattgcctg gggttttatt actgcgttat ttattcccac cggttggctg cctaacgaac
    28561 atttcgccaa aattgtcggc ccgatgatta cctatttatt gcccgtgatg attggttcta
    28621 caggtggtca tctggtcggc ggtaaacgcg gggcggtcat gggcggaata ggtactattg
    28681 gtgtgatcgt tggcgcagag atcccgatgt tccttggctc aatgatcatg gggccgctcg
    28741 gtgggttggt cataaaatat gtcgataagg cactggaaaa acgcatacct gccggttttg
    28801 agatggttat caataacttc tcattaggta tcgcggggat gctcctttgt ctgctgggtt
    28861 ttgaagtgat cggcccggcg gtgttaattg ccaatacttt cgtcaaagag tgtatcgagg
    28921 cgctggtaca tgcgggttat ctgcctctgc tgtcagtcat caatgaaccg gcgaaagtgc
    28981 ttttccttaa taatgcgatc gatcagggcg tctattatcc gctgggaatg caacaggctt
    29041 cggttaacgg taaatccatc ttctttatgg tggcctctaa cccaggtccg ggcctggggc
    29101 tgctgctggc gtttaccttg tttggtaaag ggatgagtaa acgttctgcg cccggggcga
    29161 tgattattca cttcctcggt gggatccacg aactgtattt cccatatgtg ctgatgaagc
    29221 cgctgactat tattgccatg attgcgggcg gtatgtctgg tacctggatg tttaacttac
    29281 tggacggtgg tctggtggct ggcccaagtc tggggtctat ctttgcttac ctggcactga
    29341 cgccgaaagg ctcgttcctg gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct
    29401 ttgctattac ttcgctgata ctgaagatgg aaaaaacggt ggaaacggag agcgaagatg
    29461 agtttgctca gtcagccaat gcggttaagg cgatgaaaca agagggtgca ttctcgttaa
    29521 gcagggttaa gcgtatcgcc tttgtttgcg atacggggat gggctccagt gcgatgggcg
    29581 cgaccacctt ccgtaaacgc ctggaaaaag cggggctggc aatagaagta aaacattacg
    29641 cgatagaaaa cgtgcctgcg gatgccgata tcgtcgtcac tcacgccagt ctggaagggc
    29701 gcgtgaaacg tgtgacggat aaaccgctga tattgattaa taactatatt ggcgatccaa
    29761 aactcgacac tttatttaat caattaaccg ccgaacataa acactgatta cagaggtaaa
    29821 aatgaaaacc aaagttgctg ctatttatgg caagcgggat gtccgtctgc gcgaatttga
    29881 actgccagaa attaccgata atgaactgtt agtgagtgta atttctgaca gcgtctgttt
    29941 atcgacctgg aaagcggcgt tactcggtag tgaacataaa cgcgtacccg acgatttaga
    30001 aaatcatccg gtcattaccg ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa
    30061 tctcactggc aaatataaaa aaggccagcg ttttgtattg caaccggcga tggggttacc
    30121 aagcggatat tcagcaggct acagctacga atattttggc ggtaacgcca cctatatgat
    30181 tattcccgaa atcgccatta atttgggctg cgtattaccg tatcacggct cttattttgc
    30241 tgcggcgtcg ctggtagagc ctatgtgctg cattattggt gcttatcatg ccaattatca
    30301 caccacgcaa tatgtttatg agcatcgcat gggcgtcaaa cctggcggca atattgcact
    30361 gctggcgtgt gcgggtccga tgggcattgg cgctatcgat tacgccatta acggtggcat
    30421 acaaccgtca cgggtggtgg tggtcgatat cgacgacaaa cgcctggcgc aggtgcagaa
    30481 gctgctgccg gtggatctgg cggccagtaa aggcattgag ctggtgtatg tgaataccaa
    30541 agggatgagc gatccggtcc agacgctgcg ggcgctgaca ggagatgtcg ggttcgatga
    30601 catttttgtt tatgcggcgg tgcctgctgt cgttgagatg gctgacgaat tactggcgga
    30661 agatggctgt ctgaacttct ttgccgggcc gacggataaa aacttcaaag tgccgtttaa
    30721 tttctacaac gtccattaca acagcacgca cgtagtcggt acatccggcg gttcaacgga
    30781 cgacatgaaa gaggcgattg cgttaagcgc cacggggcag ttgcagccgt cctttatggt
    30841 cacgcatatc ggtgggctgg atgcggtgcc agataccgtg ctcaatctgc cggatatccc
    30901 tggcggtaaa aaactcattt ataacggcgt gaccatgccg ctcactgcca ttgccgattt
    30961 tgccgaaaaa ggcaaaaccg atccgctatt taaagagttg gcgcggctgg ttgaggaaac
    31021 gcacggcatc tggaatgaac aggccgagaa atatctgctg gcacaatttg gcattgatat
    31081 cggggaggcc gcgcaatgat gtccctggcg tggccattat ttcgcgttac ggaacaggca
    31141 gcgttggctg cctggccgca aaccggatgt ggcgacaaaa ataaaattga tggcctggcg
    31201 gtcaccgcga tgcgccaggc attaaacgac gtcgcttttc gtggacgagt ggttatcggc
    31261 gaaggagaaa ttgaccatgc gccgatgctg tggattggcg aagaggtggg caaaggcgat
    31321 gggccagaag tcgatatcgc ggttgatccc attgaaggca cgcggatggt ggcgatgggg
    31381 cagagcaatg cgctggcggt catggctttc gcccctcggg atagcctgtt acatgcgccc
    31441 gatatgtata tgaaaaagct ggttgttaat cgactggctg ccggggtgat tgatctgtct
    31501 ttgcctctgg cggacaactt gcgtaacgtg gcgcgggcgt taggtaaacc gctggacaag
    31561 ctgcgcatgg ttacgctgga taaaccgcgc ctgagtgccg cgattgagga agcaacacag
    31621 ctgggcgtga aggtttttgc cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc
    31681 tggcaggata atccttacga tgtgatgtac accatcggcg gtgcgccaga aggcgtgatt
    31741 tctgcctgtg ccgttaaagc attaggcggg gatatgcagg ccgagctgat tgacttttgt
    31801 caggctaaag gggattacac ggaaaatcgg cagattgcag agcaggagag caagcgttgc
    31861 aaggcaatgg gcgttgacgt taaccgtgtg tactcgctcg atgaactggt aagggggaac
    31921 gatatcctct ttagcgctac gggcgtgacg ggcggcgagt tggtgaacgg tatccaacag
    31981 acggcgaatg gggtgcggac gcagacatta ctgatcggcg gcgcggacca aacgtgtaat
    32041 ataatagact ccctgcattg atggtgatct atcggaagac caatggcgaa gctgacagaa
    32101 gatgatgtgc ttgagcaact ggatgcacag gacaatttat tttcatttat gaaaactgcg
    32161 cattctattt tgctccaggg gatacgccag tttctgccgt cgctgtttgt cgataacgat
    32221 gaagagatcg tggaatatgc agtgaagccg ttactcgccc aaagcggccc gcttgacgat
    32281 attgatgttg cgctgcgttt gatttatgcg ctggggaaaa tggataaatg gctgtacgcc
    32341 gatatcacgc atttttccca gttctggcat tacctgaacg aacaggatga aacgcccgga
    32401 tttgccgatg acatgacctg ggattttatc agcaacgtca atagcataac ccgcaatgcg
    32461 atgctctatg acgcgttgaa agcgatgaag ttcgccgatt tcgccgtctg gtcagaggcg
    32521 cgttttagcg ggatggtcaa aacggcgctg acgctggcag taacgacaat cttaaaggaa
    32581 ttaacgccgt gaaaattgaa ttaacggtga atgggctgaa gatacaggca cagtaccaga
    32641 acgaagagat tgaaaatgta cataaaccgt tgctgcgtat gctggcggcc ttacagacgg
    32701 taaatccgca gcggcgtacg gtggtttttc tttgcgctcc gccggggacg ggtaaatcta
    32761 ccctaaccac cttctgggaa tatctcgcgc aacaagaccc ggagctgccc gctattcaga
    32821 cgctcccgat ggatggtttt caccattaca atagctggct ggacgcgcat caattgcgcc
    32881 ccttcaaagg cgcaccagag acatttgacg ttgcgaaact ggcggaaaat ctgcgccagg
    32941 tggtggaagg ggattgtacg tggccgcagt acgatcgaca aaagcatgat cctgttgaag
    33001 atgcgttgca cgttaccgca ccactcgtca tcgtcgaagg aaactggttg ttgctggacg
    33061 acgagaagtg gtgccagctt gcgcaatttt gtgatttctc gatctttatc aacgcgccag
    33121 ctactgcgtt gcgggagcgt ctggtggggc gcaagctggc gggaggggta tcgctggcag
    33181 atgcagaagc cttttatgac cgtaccgacg ggccgaatgt tcgccgggtg ctggaagaga
    33241 gtctgcctgc aaatttgacc ttaatgatga ccgctacggg tgaatatcgt ctggtggatt
    33301 aacgttcctg attacaccat cttccagtcg caccacacga gagaaatgac gccgggtaaa
    33361 tgcggcgtca tgactgattg ctaccacgct cgtgcctcgc tggcggcact tctccagcca
    33421 gctttcaaac acgctcaacc aattttcgtc aaaatcccga ctcggctcgt ccagcagtaa
    33481 tagtggcgga gagagtgctt ccaggcaggc gacggcgacc atccgtcgct gcgcactatg
    33541 cagatccaac gggtgcgagt tggcagtgtc tgctaactga caacattgca gggcagcatg
    33601 ggtacgttga gtgatttcat ccgccgggca tttttgcagt ttcaaaccaa atgcgatctc
    33661 gtcagcgacg gtgctgtgaa aaagttggtt ttccgcttcc tgaaacagta cgccaacctt
    33721 tgccgcgcgc tggcggtttt tcaggttttt catcgcctgt tgttgcagca taaccgtacc
    33781 ggctgtgggc gtaagaagac ctgccattac tcgtaacagc gtcgatttcc ctgcgccatt
    33841 gtcaccggtc agcgccaacc attcaccttg tttgagttgc agagaaatgt cgcaaagaca
    33901 atccgttgcc gcgccaggcc agcgataaga tatctgattt aacgttaaca tagtggctgg
    33961 agcgctccgt tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg tgtgcgatga
    34021 cgttcaaaaa gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa gcgctgcaac
    34081 agcatttcgc tggcctgcgg cgtcaggcgg ctaaaggctt catcaagaat taagagcttt
    34141 ggctgcatcg cgatggcgca ggcgatgacc acgcgctgtg tctctccacc ggagagtgtt
    34201 gccgggtgac gatggcgcaa cggctggcac tcggtcagag tcagggcggc atcaatccgc
    34261 gccataatct ctgcttcagc aagacacaga ttttctggcc caaaggcaac ttcttcttcc
    34321 acgctaaagg tacagccgga gagctgcaaa tagggcgatt gctggacgag ctggatggtg
    34381 gcggattgtt cattaagcgg gagatgccca atgggcgtac cgaggagcgt ccccgtgcct
    34441 gttatctctc ctggcagaaa gtccggatac cagcccgcca tcagctgtgc cagcgtactt
    34501 ttgccactgc cgttgtcacc aaagatggcg accatccccg gtttgacata gtgaaagtca
    34561 taacagaacg gaggatgcgt tgagtgtgtc gggcaataac ggaactgttc taacgtaacc
    34621 atatccacgc tccgaattct gtcagcatca gcaagatcat ggtgtaacgc gctacgcgtt
    34681 gtagggtgct atcggctggt gcccacaacg ttgttcgtcg gttgtggatc cgaaatgccc
    34741 gcatatccag cgccgcacca cggacagcca gatcgttaag cgcattgtgt gttagcggaa
    34801 taattaacgc gggcatcgca cgtaaacgct gataccagcc ctcatccagc ggcacgccgc
    34861 gggcacgctg cgcttcatga ataatcgcta attgccgttt taactgctca acgaccaata
    34921 acggcccggc aaacagataa gcgacgcctg gcggcaggcg agaagcaaac agagcgcgaa
    34981 taaaacattg cacggggacg tactgcatcc acaactgtga ggttgaaaca atcgccagaa
    35041 tccgcaacca gagcgtaata gcatgcgacc accgttctgg cgagcgtggc gtgccgctta
    35101 gccattctgg tagccagcca ccgtgtacca gccacagccc ggctcccagc gagaacatca
    35161 gccagacgac atattttgcc cgccgccgcg tggcctttag cgcaataagg cagaaaaagg
    35221 tcgccgcgct atagatcggc aaaatggtct gcgcgggcag gataagcgtg gtgcaggccg
    35281 ccaatgccca cagtattaat gaggtaaatg gatgcattta gcgcacggca gccattgccg
    35341 gaaaatggcg cgtggtgcga atcggcagct ggcgcagtaa aagccagaca atcaccgctg
    35401 taagaatttt atcgaccagg ttcgcgccta tcaccgtaat cgccaccgac tccaccaggt
    35461 tttgccccat cgagtgcatc caggcaacga acaaatcggc tccgctaccg gtgacaccgc
    35521 cgaacagcgc agtacgcagc ggtacagcga cgaccgttac cgcaagcgta ataatcacgc
    35581 cgctgacaac gacttttggc agggtgcgaa accaacccgc tcgcgccagc caaccggcga
    35641 ccaggccgat gaccatcgcg acgggtgcaa atgcggcggc gatcgggtcg gtcagcaatc
    35701 cccagagtaa attggtcagt aagccggtca gcattccgat caccggacca agcagtaccg
    35761 cgctaataag tgtgccgatg gagtcgagaa atataggtaa tttgaccatg ctggccagtt
    35821 gcccgccgat catattgatg gcgatagata taacgatcaa caccagtgcc tgactggaaa
    35881 agtggctacg cgccataaga aaacctcttt gaaagtagtt atttaacggg ggaaatatgc
    35941 aactcgtcgt aaccagaaag cgcgcaaggt tggcggctaa acgccagttc ctcaaggcta
    36001 ccgatcacca gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg accgccaagc
    36061 atcgtgccgt gtggatcgga aacgcagaga tgaagatgct caccactctg ttccagcgtg
    36121 ccgtttagtg agatcacttc gaatgtaccg ttcaggagcg tggtgccttc ttgcctggca
    36181 tagcgcaagg cgacgtcagt caggctaccg gtacagcctg caatccaggc ggcatgaagt
    36241 tgatgctgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg gaggaggcgc
    36301 agtgcgtaaa agcgtgcgga ggaagaatat gggtagggca atgtcatcat tggagtactc
    36361 cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc tgatataagt
    36421 cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat tctgcaaagt
    36481 ggaataaatt ctacaggggc ggggctgaaa acgtgacgta agtcagataa ttgctttctt
    36541 tgactggaca atcattcctt ttattccacg tttcgcttat cctagctgaa gcgtttcagt
    36601 cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg
    36661 gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt gcaggagatc
    36721 tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc
    36781 tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg aactggcgga
    36841 tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt ttgcatggga
    36901 agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgcg tattgcatga
    36961 acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc tcgactgcac
    37021 cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg ccaaaaaagt
    37081 gctcttttca catcctggca gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca
    37141 ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca cgaattgcat
    37201 aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca ctgtgaccac
    37261 aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg acctgcgtcg
    37321 cacccgggca gccagccagt cgatcattcc ggtcgatact aaactggccg ccggtatcac
    37381 acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat
    37441 aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt
    37501 caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg actatacgga
    37561 attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg tcgatggcac
    37621 ccaaacccgg gtcagtggcg cacacctgat caaaacgttg gtctggtgcg ataacgaatg
    37681 gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg ctttcaggta
    37741 agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc atgtctgtaa
    37801 ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg gatctgaacg
    37861 taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca
    37921 tcgaactggc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta
    37981 ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac ctgaaagaca
    38041 aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac gttgctgaag
    38101 gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa
    38161 ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca ttcggtactg
    38221 ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag
    38281 gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa cctgctcgcc
    38341 cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt ttggactccc
    38401 tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc tttatcgcgg
    38461 cacaaggcca cgatgtgggt aaatccctgt acgaagctga cctggttgac gaagctaaac
    38521 gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgcgtagca accgagttct
    38581 ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac gagcagatcc
    38641 tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat gcgaaaacca
    38701 ttctgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa
    38761 tcgtggctaa cgctatcgca gacagcgaag cgttctccat cgctggcggc ggcgacactc
    38821 tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc actggcggcg
    38881 gcgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc
    38941 gcgctaagaa gtaaaaaatc acagggcagg gaaacctgcc cttgtttcag cgcgctttta
    39001 gagcacgcat tttttcatac tctaaataat tcgagttgca ggaaggcgac aagagagtga
    39061 atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg cagcacatgc
    39121 aacttgaagt atgatgagta taaggcccga cgatacagga caagagacat gtctaagatt
    39181 tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta
    39241 gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac
    39301 gccgtactgg aaactgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt
    39361 ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc
    39421 ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg
    39481 gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg
    39541 gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc tcacatgatc
    39601 gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgt
    39661 atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac
    39721 ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga agacgttgat
    39781 tacgcataca ccgagctgag caaaatcagc ccgcgtttca ccatcgctgc gtccttcggt
    39841 aacgtacacg gtgtttacaa gccgggtaac gtggttctga ctccgaccat cctgcgtgat
    39901 tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgag cttcgtattc
    39961 cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg
    40021 aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactac
    40081 aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg
    40141 aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct
    40201 cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaaga tattcctttc
    40261 tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaaaa ttgcctgatg
    40321 cgctacgctt atcaggccta cacgtatcct gcaatatatt gaatttgcag gtttttgtag
    40381 gccggataag gcgttcacgc cgcatccggc atgacaaagc gcacattgat atctgtctga
    40441 gccccgctct gcggggattt tttcgccaaa agcaaggtga ttcagatgag aaatctgtga
    40501 cctatttggc aaaattatgc tttattgttt acccttgtca gactgcccgt cataaggcgg
    40561 cggagtgtat ttctccattt tgagtcagtt gaaaaggaat attgaatgga agatttgaat
    40621 gttgtcgata gcataaacgg cgcgggaagc tggctggtag ctaaccaggc gctgctgcta
    40681 agttatgcag taaacatcgt ggcggcactc gcgatcatca tcgttggttt gattatcgcg
    40741 cggatgattt ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga tgccactgtt
    40801 gctgattttc tttctgcatt agtccgttac ggtattatcg cctttacgct aatcgctgcc
    40861 ctgggacgcg tgggtgtaca aacagcgtca gtcattgctg tactcggtgc cgcaggctta
    40921 gctgttggtc tggctttgca ggggtcactt tctaacctgg ccgctggcgt gttacttgtc
    40981 atgttccgac cgttccgtgc cggagaatat gttgacctcg gcggcgtagc cggtactgtg
    41041 ctgagtgtgc agattttctc caccaccatg cgtactgcag acggtaaaat tatcgttatt
    41101 ccgaacggta aaattattgc cggaaatatt attaacttct cccgcgagcc agttcgccgt
    41161 aacgaattta ttattggcgt ggcgtatgat tccgatatcg atcaggttaa gcagatcctg
    41221 accaatatta tccagtctga agatcgcatt ttgaaagatc gcgaaatgac tgtgcgcctg
    41281 aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa cagcggcgat
    41341 ctgcaaaacg tgtactggga tgtgctggag cgtattaaac gtgaatttga tgccgccggt
    41401 atcagcttcc cgtacccgca aatggatgtg aactttaagc gggtgaaaga agacaaagct
    41461 gcgtaatcaa cgctgaggcc agataatact ctctggcctc tctcttatta gttattctga
    41521 ttgccaatta atattatcaa tttccgctaa taacaatccc gcgatatagt ctctgcatca
    41581 gatacttaat tcggaatatc caacgtgttt tcttattact ttcaaggtct tgatcttggg
    41641 gc
//
LOCUS       DAJVOY010000011        40609 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVOY010000011 DAJVOY010000000
VERSION     DAJVOY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.011"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(249..1106)
                     /gene="murI"
                     /locus_tag="OQL90_000554"
     CDS             complement(249..1106)
                     /gene="murI"
                     /locus_tag="OQL90_000554"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1367438.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1051..2899)
                     /gene="btuB"
                     /locus_tag="OQL90_000555"
                     /pseudo
     CDS             complement(1051..2899)
                     /gene="btuB"
                     /locus_tag="OQL90_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2953..3143)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3257..4357
                     /gene="trmA"
                     /locus_tag="OQL90_000556"
     CDS             3257..4357
                     /gene="trmA"
                     /locus_tag="OQL90_000556"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1367439.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4397..4756)
                     /locus_tag="OQL90_000557"
     CDS             complement(4397..4756)
                     /locus_tag="OQL90_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1367440.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4756..5406)
                     /gene="fabR"
                     /locus_tag="OQL90_000558"
     CDS             complement(4756..5406)
                     /gene="fabR"
                     /locus_tag="OQL90_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1367441.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5727..7127
                     /gene="sthA"
                     /locus_tag="OQL90_000559"
     CDS             5727..7127
                     /gene="sthA"
                     /locus_tag="OQL90_000559"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1367442.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7110..8027)
                     /gene="oxyR"
                     /locus_tag="OQL90_000560"
     CDS             complement(7110..8027)
                     /gene="oxyR"
                     /locus_tag="OQL90_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1367443.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8294..9667)
                     /gene="argH"
                     /locus_tag="OQL90_000561"
     CDS             complement(8294..9667)
                     /gene="argH"
                     /locus_tag="OQL90_000561"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1367444.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9728..10504)
                     /gene="argB"
                     /locus_tag="OQL90_000562"
     CDS             complement(9728..10504)
                     /gene="argB"
                     /locus_tag="OQL90_000562"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1367445.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10512..11516)
                     /gene="argC"
                     /locus_tag="OQL90_000563"
     CDS             complement(10512..11516)
                     /gene="argC"
                     /locus_tag="OQL90_000563"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1367446.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11670..12821
                     /gene="argE"
                     /locus_tag="OQL90_000564"
     CDS             11670..12821
                     /gene="argE"
                     /locus_tag="OQL90_000564"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1367447.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13173..15824
                     /gene="ppc"
                     /locus_tag="OQL90_000565"
     CDS             13173..15824
                     /gene="ppc"
                     /locus_tag="OQL90_000565"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1367448.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            16007..17740
                     /locus_tag="OQL90_000566"
     CDS             16007..17740
                     /locus_tag="OQL90_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1367449.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17955..18806
                     /locus_tag="OQL90_000567"
     CDS             17955..18806
                     /locus_tag="OQL90_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1367450.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18793..19134)
                     /locus_tag="OQL90_000568"
     CDS             complement(18793..19134)
                     /locus_tag="OQL90_000568"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1367451.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19136..20014)
                     /locus_tag="OQL90_000569"
     CDS             complement(19136..20014)
                     /locus_tag="OQL90_000569"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1367452.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19980..22277)
                     /locus_tag="OQL90_000570"
     CDS             complement(19980..22277)
                     /locus_tag="OQL90_000570"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1367453.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTPGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22328..22648)
                     /locus_tag="OQL90_000571"
     CDS             complement(22328..22648)
                     /locus_tag="OQL90_000571"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1367454.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22663..23742)
                     /locus_tag="OQL90_000572"
     CDS             complement(22663..23742)
                     /locus_tag="OQL90_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1367455.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24051..26552
                     /gene="ptsP"
                     /locus_tag="OQL90_000573"
     CDS             24051..26552
                     /gene="ptsP"
                     /locus_tag="OQL90_000573"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1367456.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSSILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26564..27226
                     /gene="fsa"
                     /locus_tag="OQL90_000574"
     CDS             26564..27226
                     /gene="fsa"
                     /locus_tag="OQL90_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1367457.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27237..28340
                     /gene="gldA"
                     /locus_tag="OQL90_000575"
     CDS             27237..28340
                     /gene="gldA"
                     /locus_tag="OQL90_000575"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCS1367458.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28614..29231
                     /locus_tag="OQL90_000576"
     CDS             28614..29231
                     /locus_tag="OQL90_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS1367459.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29258..30163)
                     /gene="yijE"
                     /locus_tag="OQL90_000577"
     CDS             complement(29258..30163)
                     /gene="yijE"
                     /locus_tag="OQL90_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1367460.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30257..32437)
                     /gene="katG"
                     /locus_tag="OQL90_000578"
     CDS             complement(30257..32437)
                     /gene="katG"
                     /locus_tag="OQL90_000578"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1367461.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32766..33656)
                     /gene="metF"
                     /locus_tag="OQL90_000579"
     CDS             complement(32766..33656)
                     /gene="metF"
                     /locus_tag="OQL90_000579"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1367462.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(34005..36437)
                     /gene="metL"
                     /locus_tag="OQL90_000580"
     CDS             complement(34005..36437)
                     /gene="metL"
                     /locus_tag="OQL90_000580"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1367463.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36440..37600)
                     /gene="metB"
                     /locus_tag="OQL90_000581"
     CDS             complement(36440..37600)
                     /gene="metB"
                     /locus_tag="OQL90_000581"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1367464.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37877..38194
                     /gene="metJ"
                     /locus_tag="OQL90_000582"
     CDS             37877..38194
                     /gene="metJ"
                     /locus_tag="OQL90_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1367465.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38378..38986
                     /locus_tag="OQL90_000583"
     CDS             38378..38986
                     /locus_tag="OQL90_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1367466.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39131..39806
                     /locus_tag="OQL90_000584"
                     /pseudo
     CDS             39131..39806
                     /locus_tag="OQL90_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39978..40250)
                     /locus_tag="OQL90_000585"
     CDS             complement(39978..40250)
                     /locus_tag="OQL90_000585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367467.1"
                     /translation="MELQLSDKGVIKMKAIQCCYFLALVSSNLMAGVMGNDNIFLSNS
                     DSAALYCVAKIFPDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(40216..40609)
                     /locus_tag="OQL90_000586"
                     /pseudo
     CDS             complement(40216..40609)
                     /locus_tag="OQL90_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
BASE COUNT         9987 a        11155 c        10100 g         9367 t
ORIGIN      
        1 gataaattgt taaagagcag tgccgcttcg ctttttctca gcggcgcggg gtgtgcataa
       61 tacgctttcc cgctacagag tcaagcattt atttttgctt tctctgtcgg aattctcagg
      121 agaaccccgc tgacccggcg gcgtgtttgc cgttgttccg tgtcagtggt ggcgcattat
      181 agggagttat tccggcctga caagaggaaa tttaaaataa ttttctgacc gcgcaacatt
      241 caaccaaatc agcctaaaac tgccagtttt tcgagcgttt cgaagccgta acgctgtaaa
      301 acgggcaata attgttcagc ttctggcgtc attgccatac aaaaggcaat attcgcatcg
      361 gcagattttg catccggggc ttcatgttct aacaaccagg ccgttcggcg agcaatcgct
      421 gcgccagaat ccactagccg ggttccctct ggcagcactt gtaacagttc ttcttgtagt
      481 agagggaaat gggtgcaacc caatacaacg gtatctggcg gctctttcat tcttaaccac
      541 gggcgtagga tacgttttag tgcatccaga gaaacatctt cgccatgtag cttcgcttca
      601 gccaactcaa ccatctctgc cgagcccagc atttctatct ggcattcatt agcgaaacgc
      661 gcgatcagct catgagtata agaacgttta actgttccgc gggttgccag taatccgaca
      721 atgccatttg ccgtcagacg tgcagcaggt ttaatcgccg gcacgacacc aacaaccggg
      781 aagtcgaact tttcgcgtaa tgcaggaagt gaaacggtac tggcagtgtt gcaagcgacc
      841 acagccagcg caaggggata acgttcttgc accgcggtga caattgccac cactcgctca
      901 acaataaacg cttcgctttt ttcgccatac gggaaagcga cgttatcgaa agcataaatg
      961 taatggagat ccggtaagag atgccggatc tcgtcataga ccgacaaccc accgacgccg
     1021 gagtcaaaca ccagcacggt gggacgtggt tcagaaggtg tagctgccag acaaggtgta
     1081 ttcccgtcct gcagtttggt agccatagac tgtctcataa tctttgtcga acaggttggc
     1141 tattttacca cgaactgtca ggtgagaggt gaccggatac gcaaccgcaa gatcccacaa
     1201 gctcacaccg cccattttaa cggtttgata aggataagat gagtaatcct tatcatagcg
     1261 agtgcctaaa tactgataag taatacccca gtcgaagtca tacaactgcc agtcgagctg
     1321 gtatttcacc tgctgtttag cacggcgtaa caacggcgtg tcggtaatcg cattgcgcgc
     1381 atcgacataa tcataactca cagtatgcgt cagtggtccg gtatcaaaat tggcggtcgc
     1441 ctcgacgccc ttaatccgcg ctttcccttc gttgtaatat ttcagggtgt gatcatcata
     1501 atcgatcaag tcactgacat cgttacgata tccggaaata cgccagttca ccccagcggt
     1561 taagccttca aacgcgcctt cccactgttt gcttttctcc gggtccagat tcggatttcc
     1621 gtagaagcca tacagttgcc ccagatttgg tgccttataa gatgtcccgt aggaagcaat
     1681 gaagcgataa ccttcgatga attcccaacc ggcgctggtt tgccaggttc catgacgacc
     1741 aaactgtgag ttatcgtcac tgcgtgctgc gccttcaaag gtaaaatcgc cgacttgttg
     1801 cagcccggtc agatagatgc cggtattacg ttgatcatat ccatcctcaa cataacctgt
     1861 acccggcgtc gtagtctgtt tctgccagtc gacacccgca ccaatactac cgtgaccaac
     1921 gatgacattg tttgcccact ggacggtgta ttgcttcatc tcatcgagcg tcgccgacga
     1981 atcataacga ccataatggg gatcgtagtt gtaatctttg ctatggctat agctggtaat
     2041 gagttgtgat ttaatcagtt cgccgttata gcgcagcccg gcgtcccaac tttggctata
     2101 gagtttacgg atatcgagca acggtgaacc gggagaataa tacgcgtcat aattggtacg
     2161 gttatcatag ccatagccgc gcacaaagcc gctccaggca tcagtaaagt tatgctccag
     2221 cgcgccataa agcgttttac ttaaaaaaac atcgttatct gtctgcgctt gcgttccggt
     2281 attaccatag gcaacaacat cataaccatg agtatgggca taatcgccca acagcgttac
     2341 ccgtgtctta tcccccagtt gttgctgcgt agagacatca tagttctgat aactattgct
     2401 tccccaccct gctgaaattt ccgttccggg gtcatcgcgc gtcgtgatga tattcaccac
     2461 cccgcctatt gcatcggaac cataaacagc ggagcgcggc ccacggatat attcaacacg
     2521 ctggacaagc gcacgcaata gggaactggc taaggtcggc agaaccactc acccccgcca
     2581 gattcaggcg tacgccatca attaacacca acacatgact ggcatttgta ccgcgaataa
     2641 aaatagatga gagctgacct gaaccgccgt tttgggtgat atcgacgccc ggaagacggc
     2701 gcagcacatc attgaccgag gtcgactgcc agcggtcgat atcctgacgg gtcacaacgg
     2761 tggttggtgc aagcacagtg ctgcgcggct gttcaaaacg gttagcagta acgacgagag
     2821 tatccgggct ggtatcctgt gcccaagcgg aaaaggctgt gacagaacac gccgtcagca
     2881 gcgaagcttt tttaatcatt gtaaagcatc cacaatagaa gaaggatgcc gcaggtttca
     2941 tcaatattac gcgatgatga gacgttggcc ggcaggtctt cgggcttgga ggggtatcta
     3001 agatactaag agatgatgac ttcccaccgg atggcagtgt ccgcataacg caatcatcgc
     3061 acctttcctt accgctgcgc gtcagctcca gattcgcact ggattcccta ttaactcaca
     3121 ggaccggcaa gtggatgcta caggttgtaa caagttactg tccagacgta gctcacaaat
     3181 aggaattcat caagatctgg acatctgatg agcaatccct acaatcgccg cgtactttaa
     3241 tttttcagga tacatcatga cccccgaaca ccttccaaca gaacagtatg aagcgcagtt
     3301 agccgaaaaa gtggtacgtt tgcaaagtat gatggcaccg ttttctgacc tggttccgga
     3361 agtgtttcgc tcgccggtca gtcattaccg gatgcgtgcg gagttccgca tctggcacga
     3421 tggcgatgac ctgtatcaca tcattttcga tcaacaaacc aaaagccgta tccgcgtgga
     3481 tagcttcccc gccgccagtg aacttatcaa ccagttgatg acggcgatga ttgcgggtgt
     3541 gcgtaataat cccgttctgc gccacaagtt gttccagatt gattacctca ccacgctgag
     3601 taatcaggcg gtggtttccc tgctatacca taagaagctg gatgatgagt ggcgtcagga
     3661 agcggaggcc ctgcgcgatg cactgcgcgc gcagaatctg aatgtgcatc tgattggtcg
     3721 ggcaacgaaa accaaaatcg agctggatca agattacatc gacgagcgtc tgccggtcgc
     3781 agggaaagag atgatctacc gtcaggtaga aaacagcttt acccagccga acgcggcgat
     3841 gaatattcag atgctggaat gggcgctgga cgtaaccaaa ggctcaaaag gcgatttact
     3901 ggagctgtac tgcggcaacg gtaacttttc attagcgctg gcgcgcaatt ttgatcgggt
     3961 attagccacc gaaatcgcta agccgtcggt tgctgctgcg caatacaaca tcgcagctaa
     4021 ccatattgat aacgtacaaa ttattcgtat ggcggcagaa gaatttactc aggcgatgaa
     4081 tggtgtgcgc gagtttaacc gcctgcaagg gatcgactta aagagttatc agtgcgaaac
     4141 catttttgtc gacccaccgc gcagcggtct ggacagtgaa accgagaaaa tggtgcaggc
     4201 gtatccgtgt attttgtaca tctcctgtaa cccggaaacg ttatgcaaga atctggaaac
     4261 attaagccag acgcacaagg tcgaacgtct ggctctgttt gatcagttcc cctacacgca
     4321 ccatatggag tgcggcgtat tactgaccgc gaagtaaaac ccatgccgga tgcgccagca
     4381 tccggcataa taccgattac tcagcaactt cctgcttacg gttacgcatc ttcgcgccaa
     4441 tccagaacac cataatgacg gagagtactg ccgggaagaa gttagagccg atatccggat
     4501 attccgcacg aactaccgtg ctgtacagca gtacgccgag aataaaacag gcagctgcca
     4561 gacccggcaa gcctaccggc atagtgcgat taagataacg ttgatgcagg cagtaaaccg
     4621 tcagcaccag ggaaataatc gggaatacag aaaatggcac aatggagcta aacagcgctg
     4681 cgaaagtacc attaatcgat aagccagcaa ctaacgccag cagcagcgta cctctatctt
     4741 gatttgcttg tttcattact cgtccttcac atttcccgga ataattgcgg ttttctcttg
     4801 ttcacggcga taccagtaat aagccccttt cgaaatcatt cgcagttgca gtaccagtcg
     4861 ctcttctaat tgccgacgtt gttcgacgcc gacgtccaac gcctcggcac ccgcactgaa
     4921 gacaattgtc accattgctt cggcttgcgc ttcagtaaac gcacgcggca tatggttttc
     4981 gagttccaga tagtccgcaa gttccgcaat gaagtgctga atttcacgcg caacggcggc
     5041 acgaaacgca gcggaggtgc cggagcgttc ccgcaataat aaccggaagg cgttaggatt
     5101 attaccgatg aactccataa atgtggagac cgaggtgcgg atcacactcc cgcctttggc
     5161 gatacgctga cgcgcctggc gcatgagttg gcgtagcatt aaaccgctct catcaaccat
     5221 ggtcagaccc agttcgtcta cgtcgcggaa atgccgataa aaagaggtgg gagcaatgcc
     5281 cgcttcacgc gccacttcac gcaaactcag gctggcgaag ctgcgttcag cacttaattg
     5341 gctaaatgcg gcttccacca gcgaacggcg ggttttttct ttttgttgcg ctcttatgcc
     5401 catcacgatg tctgaatcct tgccaaaagt actgctggca ctataccaga gaatgaacat
     5461 actggattgc tccagtattg tgaatgaacg gtaacgcaat aaaacatatt tactggccgg
     5521 ttcttttacg tacagcggaa acctgccgct taaacggaga gtatcgtcga taaaaatcca
     5581 ataaaacgtc agggcaaaag taagaaacag acaaagcaaa ggccgctcag gatatagcca
     5641 gataaatgac ggggatcaat tggcttaccc gcgataaaat gttaccattc tgttgctttt
     5701 atgtataaga acaggtaagc cctaccatgc cacattccta cgattacgat gccatagtaa
     5761 taggttccgg ccccggcggc gaaggcgctg caatgggcct ggttaagcaa ggtgcgcgcg
     5821 tcgcagttat cgagcgttat caaaatgttg gcggcggttg cacccactgg ggcaccatcc
     5881 cgtcgaaagc tctccgtcac gccgtcagcc gcattataga attcaatcaa aacccactat
     5941 acagcgacca ttcccgactg ctccgctctt cttttgccga tatccttaac catgccgata
     6001 acgtgattaa tcaacaaacg cgcatgcgtc agggatttta cgaacgtaat cactgtgaaa
     6061 tattgcaggg gaacgctcgc tttgttgacg agcatacgtt ggtgctggat tgcccggacg
     6121 gcagcgtcga aacactaacc gctgaaaaat ttgttattgc ctgcggctct cgtccatatc
     6181 atccaacaga tgttgatttc acccatccac gcatttacga cagcgactca attcttagca
     6241 tgcaccacga accgcgccat gtacttatct atggtgctgg agtgatcggc tgtgaatatg
     6301 cgtcgatctt ccgcggtatg gatgtaaaag tggatctgat caacacccgc gatcgcctgc
     6361 tggcatttct cgatcaagag atgtcagatt ctctctccta tcacttctgg aacagtggcg
     6421 tagtgattcg tcacaacgaa gagtacgaga agatcgaagg ctgtgacgat ggtgtgatca
     6481 tgcatttgaa gtcgggtaaa aaactgaaag ctgactgcct gctctatgcc aacggtcgca
     6541 ccggtaatac cgattcgctg gcgttacaga acattgggct ggaaactgac agccgcggac
     6601 agctgaaggt caacagcatg tatcagaccg cacagccgca cgtttacgcg gtgggcgacg
     6661 tgattggtta tccgagcctg gcatcagcgg cctatgacca ggggcgcatt gccgcgcagg
     6721 cgctggtaaa aggcgaagcc accgcacatc tgattgaaga tatccctacc ggcatttaca
     6781 ccatcccgga aatcagctct gtgggcaaaa ccgaacagca gctgaccgca atgaaagtgc
     6841 catatgaagt gggccgcgcc cagtttaaac atctggcacg cgcacaaatc gtcggcatga
     6901 acgtgggcac gctgaaaatt ttgttccatc gggaaacaaa agagattctg ggtattcact
     6961 gctttggcga gcgcgctgcc gaaattattc atatcggtca ggcgattatg gaacagaaag
     7021 gtggcggcaa cactattgag tacttcgtca acaccacctt taactacccg acgatggcag
     7081 aagcctatcg ggtagctgcg ctaaatggct taaaccgcct gttttaacac tttatcgaaa
     7141 tggccatcca ttcttgcgcg gatggcctct gccagctgct catagcggct gcgcagcggt
     7201 gagccaggac gataaaccag gccaatcgtg cggcgaggtt ccggcttaat acacggcaaa
     7261 taaacaaccc catcgcgttt gcgctccggc ggcacagcca gcgcaggcag taaagtgatc
     7321 ccgctacctg ccgccaccat gttacgcagt gtctccaggc tggtcgcgcg gaagtgtgta
     7381 tcttcatccg ccccggcttc aaagcagaaa cccattgcct gatcgcgcaa acagtgacca
     7441 tcttccagca tcagcagttt ttcccctgcc agatcggcca tcggtacgca ttcgcggttc
     7501 gcccacgggt gatcttcata gatagccagc aacattggct catcaaacaa cggcacttca
     7561 atgaatgctt cgctctcttt caccagcgcg aggatcacgc aatcgagttt gccgctgtcc
     7621 agttgcgcca gtaactggtg ggtctgtgct tcatgcagat acatttccag ctttggaaat
     7681 gtctggtgca gcatcgggat aatatgcggc agcaggtacg gtccaaccgt aggaatcaaa
     7741 ccaatatgca gcggtccgga catcgtctcg ccctgctggc ttgccatctc tttaaggact
     7801 ttcacctcac gcagcacggt acgcgcctga tccaccagca gcattcccgc ctgggtgaac
     7861 aacactttac ggctggtccg ctccagcaac atcacgccca gctcatcttc cagcttacga
     7921 atttggccgc taagcgtcgg ctggctaacg tggcaggaat ctgccgcacg ccgaaaatgg
     7981 cggtgttcag ccaatgccac caggtactca agatcacgaa tattcattat ccatcctcca
     8041 tcgccacgat agttcatggc gataggtaga atagcaatga acgattatcc ctatcaagca
     8101 ttctgactga taattgctca cagaaacgga gcggcacctc ttttaaccct tgaagtcact
     8161 gcccgtttcg agagtttctc aactcgaata actaaagcca acgtgaactt ttgcggatct
     8221 ccaggatccg cttttttttg ccataaaaaa gcccggcgat aagccaggct caaatttgta
     8281 catataaatg ttcttacccc aaccgcgcct gcgcaaaagc aatcgcttgg gccacctgct
     8341 gcggtgagac gccgcctttt gccgcacgct tgtcgaggca cgattgtagc gacagaatcg
     8401 gatagacatc ttcgccaatc acgtggctga atttctgcaa ctcgtcgagc ggcagatctt
     8461 ccagcggttt gccctgacga atggcttcca ccaccgcttc acccacgata tggtgcgcct
     8521 cgcggaaagg tacgcctttc gccaccaggt aatccgccag ttcggtggcg ttggcgtaac
     8581 cctgctgagc cgcttcctgg caacgtggac gtttcacctg aatgccgtcc agcaccagcg
     8641 ccgccatatg caggcagtcc agccaggtat cgaacgcgtc gaacagacct tctttgtctt
     8701 cctgcatatc tttgttgtaa gccagcggca aacctttcag cgtcatcatc atgccggtta
     8761 acgcaccctg cacccgaccg catttaccgc gaatcagctc cagcgcatcc gggtttttct
     8821 tctgcggcat taatgatgaa ccggaagtca cgcggtcaga aagctccaca aaccccgctt
     8881 cgccggtgtt aaagaaaatc agatcttcag caaaacgcga cagatgcacc atgccgatag
     8941 cggcagccga cagcagttcc aacacatggt cacggtcaga aacgctgtcc agactgttac
     9001 gggtcgccga agcaaagccc agccagcctg ctaactgttc acggtcgatt tcataggccg
     9061 ttcccgccag cgcgccacag cctaatgggc tgacatccag acgcttaagc gcatcctgca
     9121 aacggctttc atcacgcgcc agcatctcaa cataggccag gcaccagtgc gcgaacgtca
     9181 ccggctgggc gcgttgcagg tgagtgtaac ctggcattac cgcgtcctga ttgttttgtg
     9241 cggtttccac cagcgccgat tgcagctgcc ggttagccgt cagtaactcg ctaacggtat
     9301 ctttgcacca cagtttcagg tcagtcgcta cctgatcatt acggctacgc ccggtatgca
     9361 gctttttgcc taactggccc actttgtcga tcagtttgcc ttccacccag ctatggatat
     9421 cttcggcgtc gctttcaagg atttgttgtg gcctggcgcg aacatcttcc agcaacacgt
     9481 tcagcgcctc ttccagttgc gcctgctctt ctgcggttaa cacgccgacc gttaccaggg
     9541 ctttggacca ggccacagag ccaacaatat cctgctccgc cagacggtaa tcaaagcgca
     9601 gtgagtcgtt gaattgtttg aaccgttgat ctgctgcctg ggtaaaacgc ccgccccaaa
     9661 gtgccataac tctgtttcct tattttgaaa ttcaatgccg gaaagctgcg cctccggcta
     9721 acaaaactta agctaaaatc cgcgtaccca tcggcatacc gttaaacagt gccggaagct
     9781 gctccgcatg acgccaggag gcgatatcta ccggacggcc cagcgtgcgg gccgcatcca
     9841 gcgccgcgtt cactttcact atcatgccgt cagtaataat gccctgctca atcagttgtt
     9901 ctgctttcgc ggcggtcatt tcggcaatgc gttgcccttt gccgtcgaga atgccgctga
     9961 cgtcggagag caaaatcaga tccgcgccca gcgttgccgc cagcgccgtt gccgcctggt
    10021 cggcattgac gttcatcagt tgcccttcgt ctgttacgcc aatggagctg accaccggca
    10081 gataaccgtt ctccagcaag gagttgataa gcttaggcga acctggctgc gccagtccaa
    10141 catgacctaa ctcttcatca agctgggtca ctttgacgct gtcgccatca ccgagaaaca
    10201 aacctacagc cgcaatctga tgtttcttcg cccatgccaa cagggtttta tttgccgttc
    10261 ccgccagtgc tccggtgata atgtctatct gatcagcagg cgtcacccgc aggccgtttt
    10321 tctttttcac cggcagattc agccctttca tcagctcatc caccacgcaa ccgccgccgt
    10381 gcacaatcac cagcggacgc tgatgtgact cacgataatt caccagtgcg ctaaacagac
    10441 gttccagcgc ctcttcacta tccagcagta cgccgcccag tttgataatt aatggattca
    10501 tcattgcacc cttaaataag agactgcgtt tccgcatagc cgaaacgaat attggcgcac
    10561 tgtaccgctt gtgccgccgc gcctttcagt aagttgtctt cggtcgccac aataatcaga
    10621 tgctcgccct gaacggcaaa cccgatatcg caaaatggca gcccaacgac atttttcagc
    10681 gccggaacgc ctttgtcata cagccgcacc agcggtttat gggcatacgc ctgctgtaac
    10741 acttgcgcga cttgcgcctg ggtcacaccc gatttcaggc ggcaggtaat ggtttcgaga
    10801 atgccgcgcg ggaaattgcc cagatgtggg gtgaagataa cgtcagcacc gaggtgtgtg
    10861 gcgatctctg gttgatggcg atgagtaaag acgccatacg gttgcaggct aacttcacaa
    10921 aaactatttg aaatcgctgc cttacgtcct gcaccgctca cgccgctggt ggcgttaatc
    10981 gccggccact gattgaggtc aagaagatcg gcatcaatca acggtttcag cgccagctgt
    11041 gccgccgtcg gataacagcc tggcaccgca atcaaattcg cttcttttaa tttattaccg
    11101 caccactccg ccagaccgta ggctgcctgt tccagcagtt ccgggtattg atgggtaaag
    11161 ccgtaatatt tttcatagaa ggtggcgtcg ttaacacgaa acgcgccgga aaggtcgaac
    11221 accacgcagc ccgcttcaag aaactgcggc gctaaatcgt ggctaacttc atgggcggtg
    11281 gcgagaaaca ctacgtccac ccctgggcta aactcgctga tatccgacat cggctgcaac
    11341 ggcagctcaa cgatgccttt tagctgcgga tgcaaatcgg agattaactt tcccgcatca
    11401 ttgctttgcg ctgaaacagt caaagcggtt atgttcatat gcggatggcg atttacatag
    11461 gtcactagct ctgcgccagc gtagccgctg gcacccacaa tcagcgtatt caacatcggg
    11521 gctattcacc ttcttatgtc tggttgccag gttaaacgta aaacattcac cttacggctg
    11581 gtgggtttta ttacgctcaa cgttagtgta tttttattca taaatactgc atgaatattg
    11641 atactatcat gaccagaggt gtgtcaacaa tgaaaaacaa attaccgcca tttatcgaga
    11701 tttaccgcgc tctgattgcc acaccttcaa taagcgctac ggaagaggca ctcgatcaaa
    11761 gcaatgcaga tttaatcact ctgctggcgg actggtttaa agatttgggc ttcaatgtgg
    11821 aagtgcagcc tgttccagga actcgcaaca aattcaatat gctggcaagt atcggacagg
    11881 gggctggcgg cttgttgctg gcggggcata ccgatacggt gccatttgat gacggtcgct
    11941 ggacgcgcga tccgtttaca ctgacggagc atgacggcaa gctttacggc ttaggcaccg
    12001 ccgacatgaa aggctttttt gcgtttatcc ttgatgcgct acgcgatatc gacgtcacga
    12061 aactgaaaaa accgctctac attctggcga ctgctgatga agaaaccagt atggccggag
    12121 cgcgttattt tgccgaaact accgccctgc gcccggattg cgccatcatt ggcgaaccga
    12181 cgtcactaca accggtacgc gcacataaag gtcatatctc taacgccatc cgtattcagg
    12241 gtcagtcggg gcactccagc gatccagcac gcggagttaa cgctatcgaa ctaatgcacg
    12301 acgccatcgg gcatattttg caattgcgcg ataacctgaa agaacgttat cactacgaag
    12361 catttaccgt gccataccct acgctcaacc tcgggcatat tcacggtggc gacgcttcta
    12421 accgtatttg cgcttgctgt gagttgcata tggatattcg tccgctgcct ggcatgacac
    12481 tcaatgaact taatggtttg ctcaacgatg cattggctcc ggtgatcgaa cgctggccgg
    12541 gtcgtctgac ggtcgacgag ctgcatccgc cgatccctgg ctatgaatgc ccaccgaatc
    12601 atcaactggt tgaagtggtt gagaaattgc tcggagcaaa aaccgaagtg gtgaattact
    12661 gtaccgaagc gccgtttatt caaacgttat gcccgacgct ggtgctcggt ccgggttcca
    12721 tcaatcaagc tcatcagcct gatgaatatc tggagacgcg gtttattaaa cccacccgtg
    12781 agttgattat ccaggtaatt caccattttt gctggcatta aaacgtaggc cggataaggc
    12841 gctcgcgccg catccggcac cgttgccaaa ctccggtgcc gcaataatgt cggatgcgat
    12901 gcttgcgcat cttatccgac ctacaccttt ggtgttatgt atggcgattt tttaacattt
    12961 ccataagtta cgcttattta aagcgtcgtg aatttaatgg cgtaaattcc tgctatttat
    13021 tcgtttgctg aagcgatttc gcagcatttg acgtcaccgc ttttacgtgg ctttataaaa
    13081 gacgacgaaa agcaaagccc gagcatattc gcgccaatgc gacgtgaagg atacagggct
    13141 atcaaacgat aagatggggt gtctggggta atatgaacga acaatattcc gcattgcgta
    13201 gtaatgtcag tatgctcggc aaagtgctgg gagaaaccat caaggatgcg ttgggagaac
    13261 acattcttga acgcgtagaa actatccgta agttgtccaa atcttcacgc gctggcaatg
    13321 atgctaaccg ccaggagtta ctcaccacct tacaaaattt gtcgaacgac gagctgctgc
    13381 ccgttgcgcg tgcgtttagt cagttcctga acctggccaa caccgccgag caataccaca
    13441 gcatttcgcc gaaaggcgaa gctgccagca acccggaagt gatcgcccgc accctgcgta
    13501 aactgaaaaa ccagccggaa ctgagcgaag acaccatcaa aaaagcagtg gaatcgctgt
    13561 cgctggaact ggtcctcacg gctcacccta ccgaaattac ccgtcgtaca ctgatccaca
    13621 aaatggtgga agtgaacgcc tgcttaaaac agctcgataa caaagatatc gctgactacg
    13681 aacacaacca gctgatgcgt cgcctgcgcc agttaatcgc ccagtcatgg cataccgatg
    13741 aaatccgtaa gctgcgtcca agcccggtag atgaagccaa atggggcttt gccgtagtgg
    13801 aaaacagcct gtggcaaggc gtaccaaatt acctgcgcga actgaacgaa caactggaag
    13861 agaacctcgg ctacaaactg cccgtcgaat ttgttccggt ccgttttact tcgtggatgg
    13921 gcggcgaccg cgacggcaac ccgaacgtca ctgccgatat cacccgccac gtcctgctac
    13981 tcagccgctg gaaagccacc gatttgttcc tgaaagatat tcaggtgctg gtttctgaac
    14041 tgtcgatggt tgaagcgccc cctgaactgc tggcgctggt tggcgaagaa ggtgccgcag
    14101 aaccgtatcg ctatctgatg aaaaacctgc gttctcgcct gatggcgaca caggcatggc
    14161 tggaagcgcg cctgaaaggc gaagaactgc caaaaccaga aggcctgctg acacaaaacg
    14221 aagaactgtg ggaaccgctc tacgcttgct accagtcact tcaggcgtgt ggcatgggta
    14281 ttatcgccaa cggcgatctg ctcgacaccc tgcgccgcgt gaaatgtttc ggcgtaccgc
    14341 tggtccgtat tgatatccgt caggagagca cccgccatac cgaagcgctg ggcgagctga
    14401 cccgctacct cggtatcggc gactacaaaa gctggtcaga ggccgacaaa caggcgttcc
    14461 tgatccgcga actgaactcc aaacgtccgc ttctgccgcg caactggcaa ccaagcgccg
    14521 aaacgcgcga agtgctcgat acctgccagg tgattgccga agcgccgcaa ggctccattg
    14581 ccgcctacgt gatctcgatg gcgaaaacgc cgtccgacgt actggctgtc cacctgctgc
    14641 tgaaagaagc gggtatcggg tttgcgatgc cggttgctcc gctgtttgaa accctcgatg
    14701 acctgaacaa cgccaacgat gtcatgaccc agctgctcaa tatcgactgg tatcgcggcc
    14761 tgattcaggg caaacagatg gtgatgattg gctattccga ctcagcaaaa gatgcgggcg
    14821 tgatggcagc gtcctgggcg caatatcagg cacaggatgc attaatcaaa acctgcgaaa
    14881 aagcgggtat tgagctgacg ttgttccacg gtcgcggcgg ttccattggt cgcggcggtg
    14941 cacctgccca tgcggcactg ctgtcacaac cgccaggaag cctgaaaggc ggcctgcgcg
    15001 taaccgaaca gggcgagatg atccgcttta aatatggtct gccagaaatc accgtcagca
    15061 gcctgtcgct ttataccggg gcgattctgg aagccaacct gctgccaccg ccggagccga
    15121 aagagagctg gcgtcgcatt atggatgaac tgtcagtcat ctcctgcgat gtctaccgcg
    15181 gctacgtacg tgaaaacaaa gattttgtgc cttacttgcg ctccgctacg ccggaacaag
    15241 aactgggcaa actgccgttg ggttcacgtc cggcgaaacg tcgcccaacc ggcggcgtcg
    15301 agtcactacg cgccattccg tggatcttcg cctggacgca aaaccgtctg atgctccccg
    15361 cctggctggg tgcaggtacg gcgctgcaaa aagtggtcga agacggcaaa cagagcgagc
    15421 tggaggctat gtgccgcgat tggccattct tctcgacgcg tctcggcatg ctggagatgg
    15481 tcttcgccaa agcagacctg tggctggcgg aatactatga ccaacgcctg gtagacaaag
    15541 cactgtggcc gttaggtaaa gagttacgca atctgcaaga agaagacatc aaagtggtgc
    15601 tggcgattgc caacgattcc catctgatgg ccgatctgcc gtggattgca gagtctattc
    15661 agctacggaa tatttacacc gacccgctga acgtattgca ggccgagttg ctgcaccgct
    15721 cccgccaggc agaaaaagaa ggccaggaac cggatcctcg cgtcgaacaa gcgttaatgg
    15781 tcactattgc cgggattgcg gcaggtatgc gtaataccgg ctaatcttcc tcttctgcaa
    15841 accctcgtgc ttttgcgcga gggttttctg aaatacttct gttctaacac cctcgttttc
    15901 aatatatttc tgtctgcatt ttattcaaat tctgaatata ccttcagata tccttaagga
    15961 attgtcgtta cattcggcga tatttttttc aagacaggtt cttactatgc attccacaga
    16021 agtccaggct aaaccccttt ttagctggaa agccctgggt tgggcactgc tctacttttg
    16081 gtttttctct actctgctac aggccattat ttacatcagt ggttatagtg gcaccaacgg
    16141 cattcgcgac tcgctgttat tcagctcgct gtggttgatc ccggtattcc tctttccgaa
    16201 gcggattaaa attattgccg cagtaatcgg cgtggtgcta tgggcggcct ctctggcggc
    16261 gctatgctac tacgtcatct acgggcagga gttctcgcag agcgttctgt ttgtgatgtt
    16321 cgaaaccaac accaacgaag ccagcgagta tttaagccag tatttcagcc tgaaaattgt
    16381 gcttatcgcg ctggcctata cggcggtggc agttctgctg tggacacgcc tgcgcccggt
    16441 ctatattcca aagccgtggc gttatgttgt ctcttttgcc ctgctttatg gcttgattct
    16501 gcatccgatc gccatgaata cgtttatcaa aaacaagccg tttgagaaaa cgttggataa
    16561 cctggcctcg cgtatggagc ctgccgcacc gtggcaattc ctgaccggct attatcagta
    16621 tcgtcagcaa ctaaactcgc taacaaagtt actgaatgaa aataatgcct tgccgccact
    16681 ggctaatttc aaagatgaat cgggtaacga accgcgcact ttagtgctgg tgattggcga
    16741 gtcgacccag cgcggacgca tgagtctgta cggttatccg cgtgaaacca cgccggagct
    16801 ggatgcgctg cataaaaccg atccgaatct gaccgtgttt aataacgtag ttacgtctcg
    16861 tccgtacacc attgaaatcc tgcaacaggc gctgaccttt gccaatgaaa agaacccgga
    16921 tctgtatctg acgcagccgt cgctgatgaa catgatgaaa caggcgggtt ataaaacctt
    16981 ctggatcacc aaccagcaga cgatgaccgc ccgcaatacc atgctgacgg tattttcgcg
    17041 ccagaccgac aagcagtact acatgaacca gcaacgtacg cagagtgcgc gtgaatacga
    17101 caccaacgtg ctgaagccgt tccaggatgt gctgaatgac cctgcgccga agaaactgat
    17161 catcgttcat ctgctgggta cgcatatcaa atacaaatac cgctacccgg aaaatcaggg
    17221 caagtttgat ggcaataccg atcatgttcc gccaggatta agcgcagaag agctggaatc
    17281 atataacgat tatgacaacg ctaacttgta taacgatcat gtggttgcca gcctgattaa
    17341 agactttaaa gcggcagacc cgaacggatt cctggtttac ttctctgacc acggtgaaga
    17401 ggtttacgac acgccgccgc acaaaactca ggggcgtaac gaagacaacc cgacgcgcca
    17461 catgtacacc attccgttcc tgctgtggac gtcggaaaaa tggcaagcga ctcatccccg
    17521 tgatttctca caggatgtcg atcgtaaata cagcctggcg gaactgatcc acacctggtc
    17581 agatttggcg ggcttatctt acgacggtta cgatccaacc cgttcagtgg tgaatccgca
    17641 gttcaaagaa actacccgct ggattggtaa cccgtacaag aaaaacgcgc tgatcgatta
    17701 cgacactctg ccgtatggcg accaggtagg taatcagtaa ttatcagcca ggcgctattt
    17761 gccggatgcg acgcttgacg cgtcttatcc ggcctacaga tcgcggcggt aggctggata
    17821 aggcgtttac gccacatccg gcaatacaac tgccgcaatg tttacatcga actcccctca
    17881 ggccacactt ttcaggtggc cttttttaat aatgccagtt actgttttcc cgtctacata
    17941 cctaagggaa taacatgtat cacgacatca gctatctgct ctcccgcctg atcaacggcc
    18001 cgctgtccct gcgccagatt tactttgcca gcagtaacgg cccggtgccc gatcttgcgt
    18061 atcaggtcga ttttccacgg ctggaaattg tgctggaagg tgagtttgtt gataccggcg
    18121 ctggagcaac gttagttccc ggcgatgtgc tgtacgttgc cgctggtggc tggaatttcc
    18181 cacaatggaa aacacccgct accaccttta gcgtgctgtt tggcaaacag caactcggct
    18241 tcagcgtcgt gcaatgggat ggcaaacaat atcaaaatct ggcgaagcaa cacgtcgccc
    18301 gacgcggccc acgtataggt tcttttctgc tacaaacgct caatgaaatg caaatgcagc
    18361 cgcaggagca gcaaacggca aggcttatcg tagccagcct gcttagccac tgccgcgatt
    18421 tgctcggcag ccaaatccag accgcctcac gcagccaggc actatttgaa gctattcgcg
    18481 attatatcga cgaacgctat gcctccgcgc ttacccgcga atctgtcgca caggcattct
    18541 atatttcacc aaactatctc tcgcacctgt ttcaaaaaac gggggccatt ggttttaacg
    18601 aatacctgaa tcacacgcga ctggagcacg ctaagacgtt attgaaaggc tatgacctga
    18661 aggtaaaaga agtggcgcac gcctgcggct ttgtcgacag caactacttc tgccgtctgt
    18721 ttcgtaaaaa caccgaacgc tcgccgtcgg aatatcgccg acagtaccac agccaactga
    18781 cagaaaaacc gactactcca gaataagatg ggtttgctgc ggcgcagaaa gcactttgcg
    18841 caccgcgctc attacccgct gcggctcacg caggaatgcg tagatgctgc attgcacata
    18901 gcggcaatgt tcaaatcgct cggcaccggc aagctcgata tccgtaatca acagagcaac
    18961 atccgcccga cggatatcct cgtccgctaa acgattctcc gttcccagcg cgccctgagt
    19021 ttcaatgctg acgccccact tctctaactg gcacaacttt tccagccgtt ccgccgccat
    19081 ataagtatgc gccacgccgc ttacgcaggc ggttactgcc accaggtatg ccattttaac
    19141 ctcccacggt aacctgaaat ccggcccgtt ctgccatttc gcgcatcgtt gccacatcgg
    19201 ctgatgacgg cgcagcaaca tccttcatcg accatgtttt ccccagcagg cggtatttcg
    19261 gctcgccgta ctgatgaaac ggcaacagat ggatctgcct gatattcagc gggatcagca
    19321 cgtccagcgc ctgctgcata ttctcccggc tgagtgtgaa accggggatc agcggcaaac
    19381 gcgggatcac gttgacaccc tcactcacca gcaaacgcag gttctccagt acgcgtggca
    19441 ggttcatctt caccacatcc cgcgcctgag tcgcgtccat aatttttaaa tcgaacaaca
    19501 cttcatcgca caatttcgcc agcggtaaca gcttgctggc tggtgcgtct ccggcagttt
    19561 caatggcaca tgagacaccc cacagccgca gtcgctgtaa aaaacgggta gcaaactccg
    19621 cctgcattaa cacttcgccg ccagaaagcg tgacgccgcc gccggacgtg cgaaaaaaaa
    19681 tgtcatcttt catcacttcc cgttccagag cgtcaaggct gatatcgcga ccaatccgtt
    19741 caaacgcccc ggaggggcat tcatccgcat cacgcaaaca tttcgcacag tgcagacatt
    19801 tcgcctctct gcgtaccgtc tggattttgc cggagatcga ctccggatta gcacaccacg
    19861 ggcaaagatg cggacagcct ttaaaaaaga ccaccgtacg aatgccctcg ccgtcattca
    19921 gtgaataacg ctgaatgttg aaaatgcgcg ccacatcatt gaggcgcgtt tccacaacgt
    19981 tacagctgat gcgctgtccg gcggatgatg tcatcctgga tctccttcga cagttcgaca
    20041 aagaaggcgc tgtatccggc aacgcgcacc accagcccgg cataatcttg tgggcgctgt
    20101 tgcgcttccc acaacgtgtc ggcgttcacc acgttaaact gaatatgctg caacttaagc
    20161 tgggtaaacg cccgtaagaa gtcggccagt ttgcgtaatc ccgcttcacc ttccagggtc
    20221 gccggagtga atttcacgtt cagcaacgta ccgttagaca gcagcgtatt atcgagctta
    20281 ctgactgact tcagtaccgc tgttggccct tgttcgtcct ggcccagcat aggtgacaag
    20341 ccgccgtctg ccagctgttc tccggcaaaa cgaccgtctg gcgtcgcgcc aaccaccgat
    20401 cccaacggga cgtgagcaga aacggtatac gatcccggcg tgaagtaacc accgcgcggg
    20461 ttctggtatt tttccacttc tttgcagtag tggcgcaaca gttcggcgct aatgttatcc
    20521 acctcgtcga tatcgttacc atatttctca aagcggttaa ttaagcgagc gcggactttt
    20581 tcgccttctg gcgttgcaaa gttggctttt aataccgaca gcaattcgtc aaaacttaaa
    20641 cgctgttgct caaaaaccat ccctttcaac gcatggagag aatcgctcag gttggcgata
    20701 ccgatcccct gtacgccgga gaagttataa cgcgcgccgc catcggtaat atcgcgacct
    20761 ttttccagac aatcgctgat aaaagacgag agcagcggta caggtgccca gtcgcgatgg
    20821 ccgatatcac aaatattgct gccctcaacc atcagggtga tgtagtggct gatcttggcg
    20881 cggatctgct ccagtaaacc ttcataagtc agcgcagcat tgccttcatt ttcatgcagg
    20941 cagatttcca tcactttcag cagattaaac atcgcgatgt catgcaagcc gtaggttctg
    21001 ccgggaatag ataattccac acagcccact acggaatagt cgcgcgcatc ttccagcgaa
    21061 acgccacggt tgaggaacgc tggtaccacc acttcatcgt taaagatttg cggaataccg
    21121 gtgccgaggc gaatggtttc ggcggttttc atcaggaatg gcgtgtcgat caaggcgtta
    21181 gtgcgcacgc cgaggttcgg ttgcggtaat tgcacgcttt gataggcgtc aaggcaaagg
    21241 aacgaaagca cgttcaccgc gctacgtccg ttctcggtta acccgccgag cagtgcggta
    21301 tagccggtcg ggaaacctgc gaaataacgc gcgctgctgg tggagcgcaa cagcacgatg
    21361 tcgttgcatt tcacccataa agattcgagc agttctttca ggaacgccgg atcttcgccc
    21421 ggggttaaag atgcctgata gaacggcaac atatactggt cgaagcgccc taaagacagc
    21481 gaactggcgt tggattcgta ttgcagaatg atgttcatgt accagaataa ctggcacgcc
    21541 tgccagaacg tctgcggctt atgttcggcg ttatggcgag agatctccgc aatagtcagc
    21601 agttcttcgc gacgctgggc atctgtgcag tttgccgcca tcgtttccgc cagtacagcg
    21661 taacgcaaaa tgtgtttctg cgaggcttcc agcagtaaca gcgctgcctg ataaaagtga
    21721 ttctccggct gttgctgaca atgttgctgc atctgtgcca ccaactcccc cagcccgtga
    21781 ttcaacaggc gtgggtaatc aataataatg tgcccctggc ctttatccgt ctggttaatg
    21841 ctgaaaatct gcgtgctggt cgcggctttt acttcatccg tcatctgccc gttgatgaaa
    21901 tctttcatcg aacgtttttc ccagtacggg aacaactctt cacgatagat acgtttatct
    21961 tcttcgctga tagcaaagcg gtcctgcgga cgcgtcggga attgatccag ctctttcagc
    22021 agccagtaag ggtccatttc cggcgacata atcccggcgc gcggttttac ggtgcggtta
    22081 ccggcaatca gttcttcatc acgaatcgaa atttcaacat gttcaaggat atacgctgtt
    22141 gctttcgccc ggcgcaatat caccggttcg ccttcggttt gccgatggct ggcggtataa
    22201 agcagcgccc gctccagcga gatttcacgg gtattggcaa acagtgcagt tttgaggcga
    22261 gagatacgat tcgtcatgag gaactcctgt gggaaagcga acccgccctt gcgagcggga
    22321 gaagaactta agcggtttgc gccaggtgag cttcaatttt gctcataatg gcgtcagcac
    22381 gttttactgc atcgctgatg ttgacgcgca cgatggtctt gccagcaaaa cgctcttcaa
    22441 acttgatgcc gatatctttg gtcagaatca ccatatcagc gctcgccaca tcttccgcag
    22501 tcagttcatt ttccagacca attgacccct gggtttctac tttcacttcc cagcctttcg
    22561 ctttcgcggc actttccagc gcctctgctg ccatataggt atgggcaaca cctgaaggac
    22621 atgcggttac tgcaataatt ttcgtcatgg ctcgttcctc aattaattaa tttcaaaatc
    22681 caaatccagg tcgtcttctt tttcatcagt cgaagaccca tttttacgcg ccagactttt
    22741 cagcacgttc acacaaacag ccgtcaccac tgctccgaca gccactgctg cgatataacc
    22801 cagcttgcct tcaaccaccg gcagcacaat cagaccaccc cagcctgcat agcactgcgc
    22861 accgaccagc gccgcagtta ctgcaccaca aactgaaccg accatgatgg aaggaatgac
    22921 acgcagcgga tcggcagcgg cgaaaggaat cgccccttca gtaacgccaa cacagcccat
    22981 caccagtgcc gctttgccgg tttcgcgctc ttctgcggag aaatttttac gaccaatcag
    23041 cgtcgccagc cccatcccca gcggcgggat acaaataccc acggcagcga tgaccaccac
    23101 ggtataaaca ccctgagcaa cgcaaatcag catgaaggca taggctactt tgttaaccgg
    23161 accgcccata tcgaacgcca gcatcagacc catgatcacc gccagcataa caatgctgcc
    23221 ctgctgcatc ccctgaagcc actgagtcag gctgttggtc aacgccccta caggctcgcc
    23281 caagccccac atcatgatgc ctgcggtaat cagtgtgccg acgataggaa taatgaagat
    23341 aggcatcacc gagcgcagaa ctttatgcac cggaattttc ttcaggtaat gcaccacgat
    23401 gccgccgata atcccggcga tcagtgcacc aaagaagccc gcaccaaagc tgttaccaac
    23461 ccaggcaccg atagcgcacg gagccagcgc agaacgctct gcaatggagt aaccaatgta
    23521 agcggcgagg aaaggcacca tcagcgtcaa acctgcaacg ccgatatcaa acagtttttt
    23581 caaattcgga tcggctacgg catccggcac tgcgcctttg ccatacaaca tgacggaaac
    23641 cgccagcaaa ataccgcccg ataccacgaa gggaatcatg tgtgaaacgc ccgtcattaa
    23701 atgctgacgg gtatttttca ggatctgcac caactcattc ataagctgct cccgggcttg
    23761 cgaaagccca tgtccatatt tttgatggag gaaacaatag gcaatcgcag gcggagcaaa
    23821 cagtggataa aaaggccatt tactggactt ttgtaatatc agtacaaaaa tgcgatccgc
    23881 ctcataactt gcgataaacc ggaagcatgc cgcaaaacgg cgtcttgtga gacgcgtcgc
    23941 aaattgacgg ttacattaca tttgtccagc ttttggcaga tttgtcacat ggcaggccgt
    24001 tgccgtgcgc tcttatcctg taaacagaaa accgtaccgg gagagaggca atggccctga
    24061 ttgtggaatt tatttgtgag ctacctaacg gcgtacatgc gcgtccggca agccacgttg
    24121 aaacgctgtg taatactttt tcatcacaaa ttgagtggca taacctgcgc actgaccgca
    24181 agggcaacgc caaaagcgcc cttgcgctga ttggcaccga tacgctggcg ggcgataact
    24241 gccagttact gatttccggg gccgacgaac aggaagcgca ccagcgttta agccaatggc
    24301 tgcgcgatga attcccccac tgcgatgcgc cgctggcgga agttaaatct gacgaactgg
    24361 aaccactgcc ggtttcactg accaatctga atccgcaaat tatccgcgcc cgcaccgtgt
    24421 gcagcggtag tgcaggcggc attctgacgc cgatctcttc tttagatctc aatgcgctga
    24481 gtaatcttcc cgcagccaaa gacgttgacg ccgagcaatc cgcactggaa aatggcctga
    24541 cgctggtact gaaaaacatt gagtttcgtc tgctggatag cgacggtgct accagctcga
    24601 tactggaagc tcaccgatcc ctggctggcg atacatcttt acgtcagcac ttactgacag
    24661 gcgtcagcga gggattaagc tgtgccgaag cgattgtcgc cagtgcgaat cacttttgcg
    24721 aagagttcgc ccgttccagc agcagctacc tgcaagaacg tgccctggac gtacgcgacg
    24781 tctgcttcca gttactccag caaatctacg gtgagcaacg cttcccggca ccgggcaaac
    24841 tgacgcagcc cgccatttgt atggctgatg aactgactcc cagccagttc ctcgaactgg
    24901 ataaaaatca cctcaaaggt ctgttactga aaagtggcgg caccacctca cataccgtaa
    24961 ttcttgcccg ttcgttcaac attccgacac tggttggtgt agatattgat gcccttactc
    25021 cgtggcagca tcaaacgatt tatatcgacg gcaacgccgg ggcgattgtg gttgagccag
    25081 gggaagccgt ggcccgttat tatcagcaag aagcccgcgt acaggacgcc cttcgtgagc
    25141 aacagcgtgt ctggctgacc caacaagccc gtaccgctga cggtatccgc attgaaattg
    25201 ccgctaacat cgctcactcc gtggaagcgc aggccgcatt cggcaatggt gcggaaggcg
    25261 ttggtttgtt ccgcactgaa atgctctata tggatcgcac cagtgcaccg ggcgaaagcg
    25321 agctgtacaa cattttttgt caggcgctgg agtctgccaa cggacgcagc attattgtgc
    25381 gcactatgga cattggcggc gacaaacccg ttgattatct gaacattccg gcagaagcta
    25441 acccgttcct cggttatcgc gctgtgcgta tttatgaaga atacgcctcg ctgttcacca
    25501 cacaactacg gtcgatcctg cgcgcctccg ctcacggcag cctgaaaatc atgatcccga
    25561 tgatctcctc aatggaagag atcttatggg tgaaagaaaa actggcggaa gccaaacagc
    25621 aactgcgtaa cgaacacatt ccgtttgatg agaagatcca gcttggcatt atgctggaag
    25681 taccgtcggt gatgttcatc atcgatcaat gctgcgaaga gattgatttc tttagtattg
    25741 gtagtaatga cctgacgcaa tatctgctgg cagtggatcg cgataacgct aaggttactc
    25801 gtcactacaa cagcctgaat ccggcattct tgcgggcgct cgattacgcc gtgcaggcgg
    25861 tgcatcgcca gggcaaatgg attggtctgt gcggtgagct gggagcgaaa ggttccgtgc
    25921 tgccgttgct ggtcggctta gggctggatg aactcagcat gagcgcacca tcaattccgg
    25981 cggcgaaagc gcggatgacg caacttgata gccgtgagtg ccgcaagttg ctcaaccagg
    26041 caatggcctg ccgtacttcg ctggaagtgg aacacctgct ggcgcaattc cgcatgaccc
    26101 aacaggacgc accgctggtc accgccgagt gcatcacgct ggaaagtgac tggcgcagca
    26161 aagaagaagt gctcaaaggc atgaccgata acctgctgct ggcgggccgc tgccgctatc
    26221 cgcgtaaact ggaagccgac ttatgggcgc gcgaggccgt tttctctacc ggtctgggct
    26281 ttagttttgc cattccacac agcaaatcag aacacattga gcaatccacc atcagcgtgg
    26341 cgcgtctgca agcgccggtg cgctggggcg atgatgaagc gcaattcatc attatgttaa
    26401 ccctgaacaa acacgctgcg ggcgatcagc acatgcgcat tttctctcgc ctcgctcgcc
    26461 gcatcatgca cgaagaattc cgtaacgcgc tggttaacgc cgcctctgcc gacgctatcg
    26521 ccagcctgct gcaacatgaa ctggaactgt aaaaggaaac atcatggaac tgtatctgga
    26581 caccgctaac gtcgcagaag tcgaacgtct ggcacgcata ttccccattg ccggggtgac
    26641 aactaacccg agcattatcg ctgccagcaa ggagtccatc tgggaagttc tgccgcgtct
    26701 gcaaaaagcg attggtgatg agggcattct gtttgctcag actatgagtc gtgacgcgca
    26761 gggtatggtg gaagaagcga agcgcctgcg cgacgccatt ccgggcattg tggtgaaaat
    26821 cccggtgact tccgaaggtc tggccgccat taagatgctg aaaaaagagg gtattactac
    26881 gcttggcacc gcagtgtaca gtgctgcaca agggttactg gccgcgctgg caggggcaaa
    26941 atacgttgct ccgtatgtta accgcgtaga tgcccagggc ggggacggca ttcgtacggt
    27001 tcaggagctg caagcgctgt tagaaatgca cgcaccagaa agcatggtgc tggcggccag
    27061 ctttaaaacg ccgcgtcagg cgctggactg tttactggca ggatgtgaat ccatcaccct
    27121 gcccttagat gtagcgcaac aaatgctcaa cacccctgcg gtagagtcag ctatagagaa
    27181 gttcgaacac gactggaatg ccgcatttgg cactactcat ctctaaagga gcaattatgg
    27241 accgcattat tcaatcaccg ggtaaataca tccagggcgc tgatgtgatt aatcgtctgg
    27301 gcgaatacct gaagccgctg gcagaacgct ggttagtggt gggtgacaaa tttgttttag
    27361 gttttgctca atccactgtc gagaaaagct ttaaagatgc tggactggta gtagaaattg
    27421 cgccgtttgg cggtgaatgt tcgcaaaatg agatcgatcg tctgcgtggc atcgcggaga
    27481 ctgcgcagtg tggcgcaatt ctcggtatcg gtggcggaaa aaccctcgat actgccaaag
    27541 cactggcaca tttcatgggt gttccggtag cgatcgcacc gactatcgcc tctaccgatg
    27601 caccgtgcag cgcattgtct gttatctaca ccgatgaggg tgagtttgac cgctatctac
    27661 tgttgccaaa taacccgaat atggtcattg tcgacaccaa aatcgtcgct ggcgctcctg
    27721 cacgtctgtt agcggcgggt atcggcgatg cgctggcaac ctggtttgaa gcacgcgcct
    27781 gttcccgtag cggcgctacc actatggcgg gcggcaagtg cacccaggcg gcgctggcac
    27841 tggcagaact gtgctacaac actctgctgg aagaaggcga aaaagcgatg cttgccgccg
    27901 aacagcatgt agtaacccct gcgctggaac gcgtgattga agcgaacacc tatttaagcg
    27961 gtgttggttt tgaaagcggc ggactggcgg cggctcatgc ggtgcataac ggcctgaccg
    28021 ctatcccgga cgcgcatcac tattatcacg gtgaaaaagt ggcattcggt acgctgacgc
    28081 agctggtact ggaaaacgcg ccggttgagg aaatcgaaac cgtagctgca tttagccatg
    28141 cggtaggttt gccaataact ctcgctcaac tggatattaa agaagatgtc ccggcgaaaa
    28201 tgcgaattgt ggcagaagcg gcatgtgcag aaggtgaaac catccacaac atgcctggcg
    28261 gcgcgacgcc agatcaggtt tacgccactc tgctggtagc cgaccagtac ggtcagcgtt
    28321 tcctgcaaga gtgggaataa cctactccaa actcccggct tgtccgggag tttgaacgca
    28381 aaattgcctg atgcgctacg cttatcaggc ctacgcaatc tctgcaatat attgaatttg
    28441 cgtgcttttt aggccagata aggcgttcac gccgcatccg gcactctgtg ccagtaatct
    28501 gaaactccta cgttgttcag gagtttttta tccgcttctg gacattttct ctacagaaca
    28561 atcgttagcc cggaaggtcg aatctgtgac tcttattttc acggacgaac cagatgaaag
    28621 cttcactggc gctgctcagc ctgctaacgg catttaccag ccactcctta aaatctcccg
    28681 ccgttccacc aaccgtggta caaatccagg ccaataccaa ccttgccatc gcagacggtg
    28741 ccagacagca aatcggcaac acgctatttt acgatccggc gtatgtgcgg cttacctatc
    28801 ctggcggtga tgtcccgcaa gaacgcggtg tatgttccga tgtggtgatc cgtgcattgc
    28861 gcagccagaa ggtcgatttg cagaaactgg ttcacgaaga tatggcgaag aattttgccg
    28921 agtacccgca aaaatggaag ttaaagcgcc cggacagcaa catcgatcac cgtcgggtgc
    28981 ctaatctgga aacctggttt agccgccaca acaaaacacg ccccatcagt aaaaatgccg
    29041 gtgactatca agcgggcgat attgtctcct ggcgattaga taacgggctg gcgcatattg
    29101 gggtggtgtc agatggcttc gcccgcgacg gtacgccact ggtgatacat aacattggcg
    29161 caggcgcgca ggaagaagat gtgctgttta gctggcggat ggtggggcat tatcgttatt
    29221 ttgttaaata agcgatcgcc ccacatgaag aaaaaattca gatgcttttt acactgacag
    29281 cttctttttt cttacggctc accagcgcca gtgccagcac aatcagcaca ataccgctac
    29341 cttcaacggc ccccggattc tcaccgagca gccaccagga aaagagtacg ccgcaaacgg
    29401 gaacggccag cgtgcttaag ctggcaatac tggcaggcaa gtttttcaat acaaacaacc
    29461 ataagctcca cgccagcgcc gtcgccagaa tcgcactgta ggccagcgcc cagaacacgg
    29521 tgggctgcca gtcaatttca cgttgcggca ccagtaaagc gaccacactc atcaccagcg
    29581 ccgcatagag catctgccag gatgttaacg ataataaatc cacgcgcgga tgacgggtgt
    29641 ataggcgttt agcaacaatc gcgctcgccc cccaactaac accggagagg attgccagca
    29701 tggcactttt catcgaagag aaatcgagtt gccacggctg caacaccaaa aataagccga
    29761 aagcggcaat cagaatcgcg aaatattgcc cacgtcgcag gcgttcaccg agaaacaacg
    29821 cggcgaaaat caccacccag aacggcatgg tatagctcag gatcgccacc ttccccgcac
    29881 ctccgctgac caacgcccac tgcgccagac caaccatccc gcaggtttgt aacagggcaa
    29941 tggctaaggt gtatttaaac ggcgtcgggt gcattccgcg accacgtaat aaaaggacga
    30001 tgaataaaac gagagcgccg aaaatgcagc gtaaggcggt aaagtcgaag gcaccgatgt
    30061 aactggtgac ttgcttcatg aaaatccagc tataactcca gataagtgtg agcacaacca
    30121 ggccactgat tgccagtggg ttgctctttc ctgcggcaga catgagagat ccagtgtgta
    30181 gttttcctgt ggacactata cgccggtaaa gaaacttcag cgactgcctg aaagacagtc
    30241 actgaagaag gtcagattac agcaggtcga aacggtcgag gttcatcact ttcacccatg
    30301 ccgctacgaa gtctttaaca aacttctcgt gggcatcgct gctggcgtaa acttccgcta
    30361 ccgcacgcag gacagagtta gaaccaaata ccagatccgc acggctggct gtgtatttca
    30421 cttcgccggt ttcgcggtca cgtccttcga acagctcttt cgattcgtca gtcgctttcc
    30481 actcgtaacg catatccagc aagttcacga agaagtcatt gctcaatacg ccaacgcggt
    30541 cagtgaagac gccgtttttg ctgctatcga agttggcacc cagtacacgc atcccgccca
    30601 ccagcgcagt catttccggc gcggtcagcg tcagttgctg tgctttgtcg atcagcagtg
    30661 actcggtggt ggaaacgtcc agacgagcgc gatagttacg gaaaccgtca gcaattggct
    30721 ccagcagctc aaacatctca atgtcagtct gatcctgacg cgcatcaacg cgacccggcg
    30781 caaacggtac atgaatgctc aaacctgcgg cgcttgcggc tttctcaaca ccaaccacac
    30841 cagccagcac gatgatatcc gccagcgagg ctttaccaga ctctttctgg attttctcca
    30901 gaacaggcag agcacgaacg gctgcggcgt tcacatccca gtcgcgctgc ggcattaatg
    30961 ccagacgcgc accgttggca ccaccgcgtt tgtcgccacc acggaaggta gaagcagatg
    31021 cccaggccac cgataccagc tcactaacag acagaccaga atccgcaatc gcgaatttca
    31081 gatcgataat gtcctgctcg gtcgggttgt agatcggctg cggcagcgga tcttgccaga
    31141 tcagatcttc tttcggcact tccggcccga tgtagcgaga tttcggcccc atatccctgt
    31201 gcgtcagttt gaaccaggca cgggcaaagg cttcgttgaa cgcctgcgga tcgttgagga
    31261 aacgacgaga gatcttctcg aactcaggat caaaacgcag cgtcaggtcg gtcaccagca
    31321 ttgtcggttt acgtttcttc gacggatcga acggatccgg gataatttcc ggtgcgtcta
    31381 ccgcttcgaa ctggattgct ccagccggac tgcgggtctg tacccactca tatttgaaca
    31441 ggttctcgaa gaaatagttg ctccactggg tcggcgtctg ggtccagaca acttccagac
    31501 cagaggtaat ggcatctgcg ccaacgccgc tgccgtaagt gctcgcccag cctaaacctt
    31561 gttcttcaat cggtgcagct tctggatcag gacctacatt tgatgtcgga ccggcaccgt
    31621 gagttttacc cagcgtatga ccacccgcaa tcagcgccac ggtttcttcg tcgttcatgc
    31681 ccatgttgcc gaaggtcgca cggatagctg ctgccgcaga aagcggttcg ccgctgtgat
    31741 ccgggccttc cgggttaacg taaatcagac ccatctcggt tgcacccagc ggtgctttcg
    31801 ccagcgcttc cggatgacgg tgagtcagcc aggctttttc atcaccccag ttaacatcca
    31861 gatccggttc ccagacgtct tcacgaccgg caccaaaacc gaaggtacgg aagccggagt
    31921 tttccagcgc cacgttacct gcgaggataa acaggtcggc ccaggagatt ttctgaccat
    31981 atttctgttt gattggccac aacaggcgac gcgctttatc aaggcttacg ttatccggcc
    32041 aggagttcag cggtgcaaaa cgttgctgac cacgacccgc gccgccgcgt ccatcgattg
    32101 aacggtaagt acctgcgccg tgccaggcca tacgaataaa cagaccggcg tagctgcccc
    32161 agtcggctgg ccaccacggc tgagattctg tcagcagggc tttcagatct tttttcaggc
    32221 cgtagtaatc taatttgctg aattctttgc ggtagtcaaa gtcctcaccc agcgggttag
    32281 aacgattaga atgttggttt aacaggtcaa cacggagttg atttggccac cagtcgcgag
    32341 tggttgtgcc cgcccccgcg ctctggtcgt gaccgccctg atggaacggg catttgccag
    32401 tggctgtcgt gttatggata tcgtctgacg tgctcatcaa tgtgctcccc tctacagtgt
    32461 taccgttacg atacacagcg ttagagagaa gttataattg aattaatcca cggatgcgat
    32521 agttgagatc ttacatattg ttggttaaag agatgtaggt caaattgatc gtaattaagc
    32581 ttttttgatt atgtccgaat tcggacaaat gctttataaa aaaggggttt agcttcatat
    32641 ccttcaagta atgagggaga aaacaaggct gacaacctta ttttcccgcc ctcatttcga
    32701 ggcagcattt tgtgctctgt ttaaaatttg tgatcactgt gtgattttca caaaagccac
    32761 actatttata aaccaggtcg aacacccagc gtatggcaaa tcgcgtaact catttcagcg
    32821 cggttaagcg tatagaagtg gaaatctttc actccttcac ggcttaaaat cttcaccata
    32881 tccatggcaa tattcgcgcc caccagtttg cgggtttcgg catcatcatc caggccgtcg
    32941 aacatttgcg ccatccacgc cggaatacgc acgttggtca tatcggcaaa tttcttcgcc
    33001 tgtttaaagt tagataccgg caaaattccc ggaataattt ccacatcaat gcccgccgat
    33061 acacagcggt cacgaaaacg caggtagctt tcgacatcga agaagaactg agtaatcgcg
    33121 cggttggctc cggcatcgac tttgcgtttc agattaagca aatccgcctg agcgcttttt
    33181 gcttccgggt gaacttccgg atacgccgcc acggagatat cgaaatctgc cacttctttt
    33241 aatagcgtca ccaggtcaga agcatacatt tctggcttac cacttcccgg cggcagatcg
    33301 ccacgcagcg ccacgatatg acgaataccg ttattccagt agtcgcgtgc aatggtgcgc
    33361 aactcgtcgg gcgtcgcatc aatgcaagta agatgcggtg ccgcttccag accagtgcga
    33421 tctttaatgc ctttaataat gctgtgcgta cggtcgcgct cgccggagtt cgcgccatag
    33481 gtcaccgata caaacttcgg tttcaggctg ctaaggcgat cgatggagtt ccacagggtc
    33541 tgctccattt cactggtacg cggcgggaaa aactcgaacg aaacgttaat ctgcccctgg
    33601 acttctgcca ggcgctgatt cagggcatcc cgctggctgg cgtgaaaaaa gctcatacct
    33661 tacctcatca atcgcttgta gttatatgtt gtgttgtgca catctatccg tttagacgtc
    33721 cagatgtaaa gatgaaggaa aagccgaagg gcgtcaaccg aaaaatgacc aatgatggtg
    33781 aggaaaaatc agggaagatg aaaaaacttc aggatggtaa gaaaaagaaa atccggtatg
    33841 caaagcaaac cggactgcaa atataaacta accatgtact tattgcttct tgccggattc
    33901 gtcgtaaacg cttaatccgg cctaatccta tgataattca atatatttca ttagaaacca
    33961 taaacccgaa aacatgagta ccgggcatta ttaaatttct gaaattacaa caactgtgcc
    34021 agccggttga tatccgactg aatcgccccg gcggtgacgt cgcgccccgc gccaggccca
    34081 cggatcacca gcgggttgtc gcgataccag cggctttcga tggcaaagac gttatcgcac
    34141 ggcagcagtg atgccagcgg atggtcttca cgcaccgcct ccacgcccac acgcgctttg
    34201 ccgttggcat cgaaacgcgc cacgtagcgc agcaccagcc ccatttcgcg ggccgcttcc
    34261 agccgttgca ccatctgctc gttaagttca tcgccatttt caaagaaatg gtcgatgctg
    34321 ccgccttcgc aatgagcagg caccagcgat tccacacgta cctggtccgg ttcaatgttg
    34381 taacccgctt cacgcgccag aattaccagc ttgcgcatta cgtctttgcc ggagaggtcg
    34441 tcacgcggat caggctcggt taagccctgc tgccacgcct gatccaccag ctcggtaaac
    34501 ggcacgctac cgtcgaattg caggaacagc caggagagcg tgccggagaa gatcccgctg
    34561 atcgacaaaa tagtatcgcc gctgtcgatc agatcgcgca cggtgtggtt gatcggcaag
    34621 cccgcaccga cggtggcatt gtacagccag tgacgcccgg ttttttcgaa ggcgtcgtgg
    34681 atctggcgat atttattgct gtcgctggct cccgccagtt tgttggcgct gataacgtgg
    34741 aaaccgtggc tggcgaaatc aagatactga tcagcaagct gctggctggc ggtaacgtcc
    34801 agcaccacta aatcatcata cggatgggcg cgcatccaca ggaacaacga ctcttcatcc
    34861 tgctcaaccg cttcatcgtt gaagaaggct aacgcgcggc tggcgtccag cccgtcatag
    34921 ctcaacaggc tacggcggct gtccaccaca cctgccagca caaactcaaa gccggtacgt
    34981 gccgaaagcg tgctctgctc acgggcgaac agttccagcc aacgggaacc gatattgccc
    35041 ttaccgaaca ataccaggcc gatgcgtttt tctgcgcgga agacggactg atgcagcccc
    35101 tgaatcaggc tttcggtcgg gccggtgcgc agtactgcca ccaggctgat gccgtcatcg
    35161 gactgccagg taaattcgac cggctggcct ttcagttgct gccagaagcg gtggcaatgc
    35221 agcgggttac gggtgacgcc tgcaccgacc atcgccacca gcgccagccc ctgacgcagg
    35281 cgcagttcgc caggtaatcc cgcttcgtcg aggattttca gcgcactgtc ggccacttct
    35341 gaggtgtagc aaaattgcag caactggcga tcgttatgta cgccaaccgc cagcgggcgt
    35401 acctgcgcgc gtttcaggat ttggtcgatc tctttatgcg ccagtttgaa atcctgactg
    35461 gcgggcacct gaaactcaat caaacagaca tcatcgtggc tggtgacaat acgcgcacca
    35521 gtaccggagg ccagcacgcg ttcaatgcgc gtggaacctt gatccggcgt gtagctacag
    35581 cgcagttgca ggtcgatttc gctgccagaa accggctgta aagtacgggc gtgaagaacg
    35641 ggagccgcca ggcgcgccag ttcgctggcc tcatccagac gcagcaacgg cagcaggcag
    35701 gcatctttca ctttacgcgg gtcggcactg tataccccgg cgacgtcgct ccagatggtt
    35761 acgcgagaaa cacccgccag cgcaccgatt tgtgtcgcgg aatagtcgga accgttacgc
    35821 cccagcagca ccgtttcacc ggcgttgttg cggctgataa atccggtcac caccagacgt
    35881 ttgcccggat gttgcaccag cagctgttgc agcaacgggt aagaaagccc ttcatcaacc
    35941 tgcggttgtg cggcgcgttc agcgcgtaaa aactcgcggg catcaagcca ggccgctggc
    36001 agcccttgtt gattaagtac cgcagacatc aggcgtgccg accatacttc cccgtggccc
    36061 accacttccg catacactgc gtcgttaata ccgctgtcga gcagcgccgc caggcgctca
    36121 aggtcgctga caaaagcgct aatgaggcta tcagcttctt cagcgggtag cagaccgcta
    36181 atcagatcgc actgatagcg acgcagcgtt tgttgaacct gatgcgcaga gagacgatcg
    36241 gtctggctta gtttcaacca gttaatcaac tggttagtgg tgctaccggc ggcggaaacc
    36301 accatcatat cgtcaggctg agagtactcc gccataatgc ccgcaacacg caaataacac
    36361 ttcacatcag ccagactact gccaccaaat ttatgcagtt gacgaccttt cgcccctgcc
    36421 tgcgcaatca cactcatttt taccccttgt ttgcagcccg gaagccattt tccaggtcgg
    36481 caattaaatc ttcgccatct tcaataccgg tggagatacg cagcagcgtc tcggagatcc
    36541 cggcggcagc acgcgcttct ggtgccatgc ctgcatgtgt catggttgcg gcgtgagaga
    36601 ttaaactttc cactccccct aatgattccg ccagcgtaaa caacgacagc ccgcccagga
    36661 aacgacgcag cgtctgctca tcgccatcca gttcaaaact caacattgcg ccaaagcctt
    36721 tttgctggcg cgcggcaatt tcatgcccct gattttccgg cagcgacggg tgatacagtt
    36781 ttttcaccaa cggctgggtt tgcagatatt tcacaatcgc ctgcgcgttg cgctgggcca
    36841 gctccatacg cggcaccagc gttcgcaacc cgcgtagcag cagatagctg tcaaacgcgc
    36901 cgcccgtcac gccaatattg tttgtccacc aggcaagttc agtgacaacg tccgggtctt
    36961 tagcaatcac cacgccagcc accacgtcag agtgaccgtt cagatatttc gtacatgaat
    37021 gcaacaccag atcggcaccc aatgccagcg gattttgtaa tgccgggctt aagaaggtgt
    37081 tatccaccac gctcaccgcc ccgacttccc ttgccagatg gcagattttc gcaatatcca
    37141 cgacgcgtaa caatggatta cttgggcttt ctaccagtac cagtttaggt ttttctgcca
    37201 gcgctgcccg taatgcctgt tcatcgcctt gatcaacaaa caacacgcga tagcaaccgc
    37261 gtttcgccag actgtcgaac aggcgatagc taccgccgta gcagtcgtgc ggcgcaacca
    37321 gcagatcgcc aggtttcaaa aagacggtcg ttaccaagtg aatcgcggac atgccggtat
    37381 tagtaagtac tgcgccagca ccgccttcca gttctgccag cgcacgctga accacatcgc
    37441 gcgttgggtt gccgcgacgt gagtaatcat gcgcgcgcgg ttcattaaat ccggtaaagt
    37501 tataggtgct ggaaagatgg atgggaggga caacgcaacc atactgttcg tcgtcattta
    37561 acccgctacg cactgcgatg gtggcctgtt tacgcgtcat gtgatgaagt ttcctgggct
    37621 ttgtcggtga aatgtcaggc accagagtaa acattgtgtt aatggacgtc aatacatctg
    37681 gacatctaaa cttctttacg tatagattga gcaaatccca aatagccgtt aaaattatat
    37741 gcattatcac gccgacaggt gcattacacg atgtcacggt aacgcctgta cggtaaacta
    37801 tgcgggttta cggtcagtac ccacataaat tgtgtggtct ggtctcaatt tattgacgaa
    37861 gaggattaag tatctcatgg ctgaatggag cggcgaatat atcagcccat acgctgagca
    37921 cggcaagaag agtgaacaag tcaaaaagat tacggtttcc attcctctta aggtgttaaa
    37981 aatcctcacc gatgaacgca cgcgtcgtca ggtgaacaac ctgcgtcacg ctaccaacag
    38041 cgagctgctg tgcgaagcgt ttctgcatgc ctttaccggg caacctttgc cggatgatgc
    38101 cgatctgcgt aaagagcgca gcgacgaaat cccggaagcg gcaaaagaga tcatgcgtga
    38161 gatggggatt aacccggaga cgtgggaata ctaaagcaaa aaagagcggc gcggagtgga
    38221 atcgcctgat gcgctacgct tatcaggcct acgtcatatt gcaatttatt gaatttgcac
    38281 gaacttgtag gccggataag gcgttcacgc cgcatccggc ataaacaacg agcacgttgt
    38341 ctgcgaccca ccgcttttta tacatggacg tttaactatg aaaaacaggc tgctgatcct
    38401 cagcctgctg gtttctgtac ctgcctttgc ctggcagcca caaaccggcg acatcatctt
    38461 tcagatctct cgctcatcgc aaagtaaagc gatccaactg gcgacccatt ccgattatag
    38521 ccacaccggt atgctggtga tacgcaacaa aaagccctac gtttttgaag cggtcggccc
    38581 ggtgaaatac accccgctca agcagtggat cgcccatggt gaaaagggca aatacgttgt
    38641 tcgccgcgtt gaaggcggac tgagtgttga acaacagcaa aaactggcgc aaacggtaaa
    38701 acgttatctc ggtaaaccgt atgatttcag cttctcatgg agcgacgatc gccagtactg
    38761 ttcggaagtg gtgtggaagg tttaccagaa cgcgctggga atgcgcgtgg gcgagcagca
    38821 gaagttgaaa gagttcgatc tcagcaaccc gctggtccag gctaagctca aagaacgcta
    38881 cggcaaaaat attccgcttg aggaaacggt agtttcacca caagccgttt tcgacgcgcc
    38941 acaactcacc acggttgcca aagagtggcc gctgttttcg tggtaatgga tgatgaaaaa
    39001 aataattccc gccgtagtct gtttgttgct ttgtagttgc acgacctttc cgcaaactgt
    39061 cgctccggta aacggtgatg cctccaatta gttgaattaa ggatataata ctcgctttta
    39121 aggttgtttc atggtcacag ttaaccttca ttgcccccgt tgccagtccg ttcaggttta
    39181 ccgtcacggg cagaacccaa aaagacatga tcgattccgc ggccgtgact gtcaccgggt
    39241 atttcagctc agttactgtt acgagccccg taaaccggga gttaaagacc aaatcgctga
    39301 aatgactttc aacggtgccg gggttcgcga tactgcccgt acgctgaaaa tcggcattaa
    39361 tacagtcatc cgaactttaa aaaactctcg ccacgaagaa taacgtcatc cccggtcgct
    39421 catgctgatg tggcactcat ctgcgaactt agtcagcaat ggagctttgt cggcagtaaa
    39481 gcgcgacagc actggctctg gtacgtgtac aacacgaaaa ctggtggtgt tctggcctac
    39541 acttttggcc cccggactga cgaaacctgt cgtgaattgc tggcgctgct taccctttta
    39601 ccatcggcat gttgaccact gacgaatggg gtagttacac cagagagtta ccgaaggaaa
    39661 agcatctgac tggtaagatt tttactcagc gcattgagcg caacaatctg accctgagaa
    39721 cccgaatcaa acgtctggcc cgcagaacca tctgtttctc gcgctctgta gaacttcatg
    39781 aaaaagtcat cggtgctttt atcgaaaata tatgttctac taattggagt catcaccctt
    39841 ctgatgagct ttccttggat gattaccaga ctgaatacac agccaaagat ttttacctca
    39901 ttctgctctt ttttctcaaa tgacatatat tatattttac atcatattca taggcatatg
    39961 acattccccc gcccccatta tgtcattgtg ggttgatgtt gggctttcat aaaaaaaaca
    40021 tctttttttg aactagcatt aattatattt actttatata ttaaaatatt ttctccatct
    40081 ggaaatattt tagccacaca atataaagca gctgaatccg aattacttaa aaatatatta
    40141 tcattaccca taactccagc cattaaatta ctggagacta aagcaaggaa ataacaacat
    40201 tgtattgcct tcattttaat tactcccttg tctgataatt gtaactccat tccctttagc
    40261 tttgggatta tattttagat aggtgttatt tggtgttccc aagtaactac ttgtattctt
    40321 tttttcccat tccattcata taagaattcg ttaaatcttt gcttgaaaaa tttagactat
    40381 cataaacatc attttctttt gttactttat ttcctttatc gtcagagtaa aaaccgtgag
    40441 tgtgataatc gcctactctc tctgaacctt cagggcattt tatatttcgc aagtctacac
    40501 tatcattatc atttgaactt atcggattca tggggaaata ttcaccttgc ttcttacata
    40561 ttagtccacc atattcttta ttctcgttaa ttgattctcc attacacat
//
LOCUS       DAJVOY010000012        40299 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVOY010000012 DAJVOY010000000
VERSION     DAJVOY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.012"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(52..2373)
                     /locus_tag="OQL90_000587"
     CDS             complement(52..2373)
                     /locus_tag="OQL90_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1367468.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2470..2946)
                     /gene="greB"
                     /locus_tag="OQL90_000588"
     CDS             complement(2470..2946)
                     /gene="greB"
                     /locus_tag="OQL90_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1367469.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3174..3893
                     /gene="ompR"
                     /locus_tag="OQL90_000589"
     CDS             3174..3893
                     /gene="ompR"
                     /locus_tag="OQL90_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1367470.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3890..5242
                     /gene="envZ"
                     /locus_tag="OQL90_000590"
     CDS             3890..5242
                     /gene="envZ"
                     /locus_tag="OQL90_000590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1367471.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5318..6940)
                     /gene="pckA"
                     /locus_tag="OQL90_000591"
     CDS             complement(5318..6940)
                     /gene="pckA"
                     /locus_tag="OQL90_000591"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1367472.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7319..9043
                     /locus_tag="OQL90_000592"
     CDS             7319..9043
                     /locus_tag="OQL90_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1367473.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9178..10056)
                     /gene="hslO"
                     /locus_tag="OQL90_000593"
     CDS             complement(9178..10056)
                     /gene="hslO"
                     /locus_tag="OQL90_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1367474.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10081..10482)
                     /gene="hslR"
                     /locus_tag="OQL90_000594"
     CDS             complement(10081..10482)
                     /gene="hslR"
                     /locus_tag="OQL90_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1367475.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10493..11161)
                     /gene="yrfG"
                     /locus_tag="OQL90_000595"
     CDS             complement(10493..11161)
                     /gene="yrfG"
                     /locus_tag="OQL90_000595"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1367476.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11226..13361)
                     /gene="igaA"
                     /locus_tag="OQL90_000596"
     CDS             complement(11226..13361)
                     /gene="igaA"
                     /locus_tag="OQL90_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1367477.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13681..14241
                     /gene="nudE"
                     /locus_tag="OQL90_000597"
     CDS             13681..14241
                     /gene="nudE"
                     /locus_tag="OQL90_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1367478.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14406..16958)
                     /gene="mrcA"
                     /locus_tag="OQL90_000598"
     CDS             complement(14406..16958)
                     /gene="mrcA"
                     /locus_tag="OQL90_000598"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1367479.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17078..17857
                     /gene="hofM"
                     /locus_tag="OQL90_000599"
     CDS             17078..17857
                     /gene="hofM"
                     /locus_tag="OQL90_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1367480.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17857..18396
                     /gene="hofN"
                     /locus_tag="OQL90_000600"
     CDS             17857..18396
                     /gene="hofN"
                     /locus_tag="OQL90_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCS1367481.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18380..18820
                     /gene="hofO"
                     /locus_tag="OQL90_000601"
     CDS             18380..18820
                     /gene="hofO"
                     /locus_tag="OQL90_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1367482.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18810..19214
                     /gene="hofP"
                     /locus_tag="OQL90_000602"
     CDS             18810..19214
                     /gene="hofP"
                     /locus_tag="OQL90_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS1367483.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDVDTRRTGGKAAKSDADGG"
     gene            19126..20364
                     /gene="hofQ"
                     /locus_tag="OQL90_000603"
     CDS             19126..20364
                     /gene="hofQ"
                     /locus_tag="OQL90_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1367484.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20765..21286
                     /gene="aroK"
                     /locus_tag="OQL90_000604"
     CDS             20765..21286
                     /gene="aroK"
                     /locus_tag="OQL90_000604"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1367485.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21343..22431
                     /gene="aroB"
                     /locus_tag="OQL90_000605"
     CDS             21343..22431
                     /gene="aroB"
                     /locus_tag="OQL90_000605"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1367486.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22523..23809
                     /gene="damX"
                     /locus_tag="OQL90_000606"
     CDS             22523..23809
                     /gene="damX"
                     /locus_tag="OQL90_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1367487.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23916..24752
                     /gene="dam"
                     /locus_tag="OQL90_000607"
     CDS             23916..24752
                     /gene="dam"
                     /locus_tag="OQL90_000607"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1367488.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24770..25447
                     /gene="rpe"
                     /locus_tag="OQL90_000608"
     CDS             24770..25447
                     /gene="rpe"
                     /locus_tag="OQL90_000608"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1367489.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25440..26198
                     /gene="gph"
                     /locus_tag="OQL90_000609"
     CDS             25440..26198
                     /gene="gph"
                     /locus_tag="OQL90_000609"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1367490.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26191..27195
                     /gene="trpS"
                     /locus_tag="OQL90_000610"
     CDS             26191..27195
                     /gene="trpS"
                     /locus_tag="OQL90_000610"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1367491.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27464..28369
                     /locus_tag="OQL90_000611"
     CDS             27464..28369
                     /locus_tag="OQL90_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367492.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28386..28748
                     /locus_tag="OQL90_000612"
     CDS             28386..28748
                     /locus_tag="OQL90_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1367493.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28832..29995
                     /locus_tag="OQL90_000613"
     CDS             28832..29995
                     /locus_tag="OQL90_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCS1367494.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            29995..31221
                     /locus_tag="OQL90_000614"
     CDS             29995..31221
                     /locus_tag="OQL90_000614"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1367495.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31218..32096
                     /locus_tag="OQL90_000615"
     CDS             31218..32096
                     /locus_tag="OQL90_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1367496.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32107..32460
                     /locus_tag="OQL90_000616"
     CDS             32107..32460
                     /locus_tag="OQL90_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1367497.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32472..33776
                     /locus_tag="OQL90_000617"
     CDS             32472..33776
                     /locus_tag="OQL90_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1367498.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33788..34873
                     /locus_tag="OQL90_000618"
     CDS             33788..34873
                     /locus_tag="OQL90_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1367499.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35025..35756)
                     /gene="phoB"
                     /locus_tag="OQL90_000619"
     CDS             complement(35025..35756)
                     /gene="phoB"
                     /locus_tag="OQL90_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1367500.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35856..36641)
                     /gene="frlD"
                     /locus_tag="OQL90_000620"
     CDS             complement(35856..36641)
                     /gene="frlD"
                     /locus_tag="OQL90_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1367501.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36638..37468)
                     /gene="frlC"
                     /locus_tag="OQL90_000621"
     CDS             complement(36638..37468)
                     /gene="frlC"
                     /locus_tag="OQL90_000621"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCS1367502.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37518..38540)
                     /gene="frlB"
                     /locus_tag="OQL90_000622"
     CDS             complement(37518..38540)
                     /gene="frlB"
                     /locus_tag="OQL90_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1367503.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38561..39949)
                     /gene="frlA"
                     /locus_tag="OQL90_000623"
     CDS             complement(38561..39949)
                     /gene="frlA"
                     /locus_tag="OQL90_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCS1367504.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            complement(40194..>40299)
                     /locus_tag="OQL90_000624"
     CDS             complement(40194..>40299)
                     /locus_tag="OQL90_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4223 family protein"
                     /protein_id="HCS1367505.1"
                     /translation="GHIENRDKNCSYDYLLHPAISISKIIGGCGPTAQ"
BASE COUNT         9311 a        10226 c        11090 g         9672 t
ORIGIN      
        1 agatgcggcg tgaacgcctt atcgcgcagg tttaggggcc agtccgggcg tttaacgttt
       61 tttgcccatt gccgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc
      121 acgaccgcgt ggtttggctg ccgggcggtt gttttgcggg cgttcattac cgccgccgcg
      181 acgggcgttg gtttcgccag gctgctcgtc cagacgcata gtcagggcga tacgtttacg
      241 ctgaagatcc acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg
      301 cggatcttcc acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac
      361 gccgatatcg acaaacgcgc caaagttggt gacgttggtc accgcacctt cgaggatcat
      421 acccggttgc aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc
      481 cggacgcgga tcgcgacccg gtttttccag ctctttgatg atgtcagtta ctgtcggcac
      541 accgaatttt tcatcagtaa agtcagacgc tttcaggtta cgcagttcgc tgctgttacc
      601 catcagatct ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc
      661 ttccgggtgg acggtagaag catccagcgg gttatcaccg tggttaatgc gcaagaagcc
      721 cgcgcactgc tcgaaggctt tcggccccag acggctgact ttcagcagtt gctgacggtt
      781 ctggaactgg ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag
      841 gcccgccacg cgggttaaca gcggaacaga agcagtgttg agatcgacgc caacggcgtt
      901 tacgcagtct tctactactg cgtccagttt gcgggccagt tgcgtctggc tgacgtcatg
      961 ctgatactga cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc
     1021 ctgcaaacgg cgggcgatag acaccgcgcc acgcagcgaa acgtcgagat ccgggaactc
     1081 ctgcgctgcc agctcggaag ctgagtaaac cgacgcgcca gcttcgctga cgatcacttt
     1141 ctgtgcggtc actttcggga actgcttctg cacgtcgaga tagaaacgtt cagtttcgcg
     1201 ggaagctgta ccgttgccga tcgccaccag ttcaacgtta tgtttttcac acaaggcagc
     1261 aacggtcatc gctgcttttg cggcttgtcc ggtgtgcggg taaatggtat cggtcgctac
     1321 cagtttgcca gtggcatcga ccaccgccac ttttacccca gtacgcagac ccggatcgag
     1381 gcccatcgtt gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc
     1441 aaagacgttg attgcttcat cttccgcgcg ttcgcgcacg gtacccatca gttcggtttc
     1501 cagatgcatc agcaccttga tgcgccaggt ccagctcact acgcctttgc gccagctatc
     1561 cgccggggca ttgttcaggc gcaggccaag gtgatccatg atgatttgct cgcaatagct
     1621 ctctttgggc ggctcatcga actgcggatc ggcattcagc gaaagctgga gtacgccttc
     1681 gttacgccca cggaacatcg ccagagcgcg gtgagaaggc accgtggaca acggttcgtg
     1741 atgatcgaaa tagtcgcgga atttcgcccc ttcctcttct ttaccgctca ccaccgtaga
     1801 aaccaaatgc gcgttcttcc acagataatc acgcactttc gccagcagcg cggcatcttc
     1861 ggcaaaccgt tccatcagga tatagcgcgc gccatccagc gcggctttgg tatctgccac
     1921 gcctttatcg gcatcaacat attgtgcagc ggcgacttct ggcgtgtgtg acggatcgct
     1981 ccacagcagg tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg
     2041 gcgtttaggt ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gggtggcgtt
     2101 gatggccttc gccagatcat cggtgagttt gccttgctcg gaaatggact tgaggatcgc
     2161 ctgacgtctc tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg
     2221 cgtgtcatcc agaccgccgg tgatttcctt acgataacgt gtgataaacg gcacggtatt
     2281 cccttcgtca agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc
     2341 cgcaataatg cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa
     2401 aatcagggga tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa
     2461 gactcttcct tacggtttca cgtactcgat agcattaaca taccagctcg cttccccggc
     2521 aggggtattc accaccgcca gatcgccgac ttcttttttc agcaatgcgc gggccatcgg
     2581 ggaatcgata gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa
     2641 acggtgagtc acgccatcgt cgttttcaat ctccacccac gcgccaaaaa agactttgcc
     2701 ttcctgctga ggggagtaat cgacgatttt gagattttcc atgcatttag tgagatagcg
     2761 cacgcgacgg tcgatttcac gcagacgctt tttattatac tgatagtcag cattttcgct
     2821 gcggtcgccc agacttgcgg cccaggtcac cttttttgtg acctccgggc gttcttcacg
     2881 ccagagataa ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt
     2941 tttcatctcg ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc
     3001 atatcaacag aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat
     3061 atgctttgta acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc
     3121 ttttttaaga atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag
     3181 agaactacaa gattctggtg gtcgatgacg acatgcgcct gcgtgcgctg ctggaacgtt
     3241 atctcaccga acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc
     3301 tgctgactcg tgaatctttc catcttatgg tactggattt aatgttacct ggtgaagatg
     3361 gcttgtcgat ttgccgacgt cttcgtagtc agagcaaccc gatgccgatc attatggtga
     3421 cggcgaaagg ggaagaagtg gaccgtatcg taggcctgga gattggcgct gacgactaca
     3481 ttccaaaacc gtttaacccg cgtgaactgc tggcccgtat tcgtgcggtg ctgcgtcgtc
     3541 aggcgaacga actgccaggc gcaccgtcac aggaagaggc ggtaattgct ttcggtaagt
     3601 tcaaacttaa cctcggtacg cgcgaaatgt tccgcgaaga tgagccgatg ccgctcacca
     3661 gcggtgagtt tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg
     3721 ataagctgat gaaccttgcc cgtggtcgtg aatattccgc aatggaacgc tccatcgacg
     3781 tgcagatttc gcgtctgcgc cgcatggtag aagaagatcc agcgcatccg cgttacattc
     3841 agaccgtttg gggcctgggc tacgtctttg taccggacgg ctctaaagca tgaggcgatt
     3901 gcgcttctcg ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt
     3961 cgccagcctg gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca
     4021 gcagtttaat aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact
     4081 ggaggacggc acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct
     4141 ggggatctct ctctactcca acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca
     4201 ctatgaattc ttaagccatc agatggcaca gcaactgggc ggcccgacgg aagtgcgcgt
     4261 tgaggtcaac aaaagttcgc ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg
     4321 ggtacgcgtg ccgctgaccg aaattcatca gggcgatttc tctccgctgt tccgctatac
     4381 gctggcgatt atgctattgg cgataggcgg ggcgtggctg tttattcgta tccagaaccg
     4441 accgttggtc gatctcgaac atgctgcttt gcaggtcggt aaagggatta ttccaccgcc
     4501 gctgcgtgaa tatggcgcgt ccgaggtacg ttcggttacc cgcgccttta accatatggc
     4561 ggctggtgtt aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcatga
     4621 cttacgcacg ccgctgacgc gtattcgcct ggcgaccgag atgatgagcg aacaggatgg
     4681 ctacctggcc gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt
     4741 tatcgactac ctgcgcaccg ggcaggagat gccgatggaa atggcggatc tcaacgcagt
     4801 actcggtgag gttattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta
     4861 ccccggcagc attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat
     4921 ggtggtcaac gccgcccgtt acggcaatgg ctggatcaaa gtcagcagcg ggacggagcc
     4981 gaatcgcgcc tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa
     5041 gcacctgttc cagccgtttg tccgcggcga cagtgcacgc accattagcg gcacgggatt
     5101 agggctggca attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac
     5161 cagcgagcgg ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca
     5221 gggcacgaca aaagaagggt aaataaacgg gaggcgaagg tgcctcccgt tttgctttct
     5281 ataagatact ggatagatat tctccagctt caaatcatta cagtttcgga ccagccgcta
     5341 ccagcgcggc acccgcaggg gtgtcggtgt atttatcgaa gttgtcgata aacagtttcg
     5401 ccagggtttc ggctttttcc tgccactgtt ccggagaagc gtaggtgtta cgcggatcga
     5461 gaatcttcgt gtctacgccc ggcagttcgg ttgggatcgc caggttaaac atcggcagag
     5521 tgaaggtttc tgcattatcc agcgaaccgt tgaggatggc gtcgataatg gcgcgggtat
     5581 ctttaatcga gatacgtttg cctgtgccgt tccagccagt gttaaccaga taagcctgcg
     5641 cgcccgccgc ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca
     5701 ggaatgccgc gccgaagcaa gcggagaagg ttggcgtcgg ttcggtgatg ccacgctcag
     5761 taccggccag tttggcggtg aagccagaga ggaagtgata ctgggtttga tcggcagtca
     5821 ggcgagaaac cggcggcaac acgccgaaag catcagcagt caggaagata accttagtcg
     5881 cgtggcccgc tttggaaacc ggcttaacga tgttatcgat gtgatagatt ggataagaaa
     5941 cgcgggtgtt ctcggttttt gaaccatcat caaagtcgat agtgccatct tcacgcacgg
     6001 tgacgttttc cagcagcgca tcacgacgga tagcgttgta gatttcaggt tccgcttctt
     6061 tcgacagctt gatagttttt gcgtagcagc cgccttcgaa attaaacacg ccgtcatcgt
     6121 cccagccgtg ttcgtcatcg ccaatcaggc ggcgtttcgg gtcggtggaa agggtggttt
     6181 taccggtgcc ggaaaggccg aagaacaccg caacatcgcc tttctcgcca acgttggcgg
     6241 agcagtgcat agaagcgata cctttcagcg gcagcaggta gttcatcatc gagaacatcc
     6301 ctttcttcat ttcgccgccg taccaggtgc cgccaatcag ctgcatgcgc tcggtcaggt
     6361 taaacgccac gaagttttcg gagttcagac cctgttcttt ccactgcggg ttagtgcact
     6421 tcgcgccgtt cataacgata aagtctggtt tgaaacctgc cagctcttca tcgctcgggc
     6481 gaataaacat gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg
     6541 aaagacgagt atccgggttc gcaccacaga aagcgtcgac aacgaacaga cgtttgccgg
     6601 aaagctgcct ggtcaccagg cctttcagat gctgccaggt ttccggagag agaggtttgt
     6661 tgtcgttctt acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac
     6721 ggacgatata cttatctttt ggtgaacgac cggtgaagat cccggtatcg acggcaacgg
     6781 cacccagatt agttaacacc ccgcgctcat aacctgtcag gctcggatcg agctcttcct
     6841 gatacagcag gtcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag
     6901 cctcgagttc ttgcggggtc aaaccattgt taacgcgcat ttcactgctc cttagccaat
     6961 atgtattgcc tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat
     7021 ttacgtatct ggagaaattc atatttgaca aaaaaccccg tgattcctgt tacgaaacgg
     7081 ttgctattat cgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct
     7141 tggtttatat ggcgggaata ttgtgagtgg aatcgcattc cttaatgaaa ttgatgcaaa
     7201 ttcaatcggt aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag
     7261 caaaatacta ctgcccggat gcgataatta acccaccttt tgattctgga taaccaacat
     7321 ggataacgtt gaactttcac cggcaacacg ctgggggatg attgctaccg gattacttca
     7381 ggggctggtt tgctacctgt tgatcgcctg gcttgccgga aaaaatcaca gttggattgt
     7441 ttatggcgtg cctgcgaccg ttgcgttttc atccgtttta ctcttctccg tgatctcttt
     7501 taaacagaag cgcctttggg gatggctggc gctggtgttt attgccacga cagggatgag
     7561 cggttggctg aagtggcaaa ctgatggcat gaacccctgg agagctgaaa aggcgatttg
     7621 ggattttggc tgctatctgc tgttgatggc aatgttgttg ctaccgtgga tacaacaaag
     7681 ccttcgtatc cgcaattgca gtagccgcta tagctacttt taccagtcag tatggcataa
     7741 cgtacttata ttactggtga tttttctcgc caatggcctg acgtggctgg tgcttttact
     7801 gtggggtgag ttgtttaaac ttgttggcat caagtttttc aatacgctct tttttgcaac
     7861 cgactggttt atgtatctta cattaggtct ggttaccgca ctggcggtga ttctcgcgcg
     7921 cacacagtca cgtttaatcg actctattca aaagttgttc acgttaatcg ccacggggtt
     7981 gctgccgtta gtatcattgc taaccctgat gtttatcatc actctgccgt ttacgggcct
     8041 gagcgcgatt tctcgccaca tctccgccgc cgggttgctg ttaacgctgg cctttttgca
     8101 attgatctta atggctattg tccgcgatcc gcaaaaagcg tcacttccct ggacggggcc
     8161 gttgcgttgc ctgattaaaa ccgctttgct ggttgctccg ctgtatgtgt tcgtcgccgc
     8221 ctgggcgtta tggctgcggg tcgctcagta cggctggact gtcgaccgct tgcagggcgc
     8281 gctggcggtg ctggtgttac tggtgtggtc gctggggtat tttgtcagta tcgtctggcg
     8341 taatgggcaa aatccccttg ttcttcaggg caaagtgaac cttgcggttt cgttattggt
     8401 gttggtgata ctggtgcttc ttaattcgcc ggtgctggac agtatgcgta ttagcgtgaa
     8461 cagtcatatg gcgcgttatc agagcggcaa aaacacgcca gaccaggtaa ctatctacat
     8521 gctcgaacag agcggtcgct atggacgtgc ggcgcttgag tcgctgaaaa gcgatgccgg
     8581 gtttatgaaa gacccgaaac gcgcgcggga tctgctgatg gcgttagatg gagagcaaca
     8641 tcttcaggaa caggtatcgg aaaaagtatt agccgaaaat gtgttaattg cccctggttc
     8701 tgttaaaccg gatgtgacat tctggtcggc cttaatccag gatcgctata acgtgatgac
     8761 ctgtattgaa aaagacgcct gcgtcctggt cgagcaagat ctgaatagtg atggtcaggc
     8821 ggagcggatc ctgtttgctt ttaatgatga cagagtcatt gtctatggct ttgactcaga
     8881 cagaaaagaa tgggacgcgc ttgatatgag tttacttccg aacgaaataa cgaaagaaaa
     8941 attactcaca gctgcgaagg atggaaaact ggggacgagg ccgaaagcgt ggcgcgatct
     9001 gacggtagat ggtgaaacgc tggaaataaa cttaagtaag tgacaacgct tttatcggat
     9061 acgacgctgt atctgaccaa aataatttgc gatttatttc ataggcgctg gtaagcgcag
     9121 cacatcaggc cattaagttg taaaatccgg cagagccctc tctgccggac atactcatta
     9181 atgaacttgc ggatctgccg gagacgcgtt gttgcggatt tcagcaatat ccatcgcatt
     9241 gaacagatag tggttaccgc agtaatcaca atgcatgtca atttcgccat cttccgccag
     9301 gatgctatca acttcttcat caggcagcgt tttcagcgca tcggcgcaat gttcacgcga
     9361 gcaggtgcat ttgaactcca catcctgcgg atcgtaaacc gtcacctctt cttcgtgata
     9421 caaacgccac aacacttcgt ttgccggtaa ggtcagcagt tcttcggttt tgatggtttc
     9481 ggttagcgtc gccaggtggt caaagtcgtc ctgctgggca ttttgcgcag gcattacctg
     9541 caacaacata ccgcctgcag ccggtttgcc gtctacgtcg ccggtgcgaa taaacaggcg
     9601 cgtcggcagc tgttcagaac gcataaagta atcttccagg caggccgcca gggtatcacc
     9661 ttccagacca actacgccct gatagcgttc gccttcgctc ggggtaatgg tgatcaccac
     9721 gtaaccattg ccgaccagcg ttttcaggtc ggcattttct ggaatttcgc cctgcacgcg
     9781 cgccacaccg cgcatttgct ggttattgtt accgttaata accgccagat tcatcggacc
     9841 gtcgccctgc agctgtacgg tgatatcacc atcaaacttc agcgtagcgg ttaacaggct
     9901 ggtcgcaacc agcagttctg ccagcacgtt tttaacgggc tgcggataat cgtggttctc
     9961 aaggatctgt tgcagggttt ccgaaacggt taccagttcg ccgcgaacgg caaaattttc
    10021 aaacagatag cgatgtaatt ggtcatgttg cggcataatc atctctcttg caggtgacag
    10081 ttattcactg tcgccgtgtt taaatcgtaa caggtcgcgg cgctcttttt tgtccgggcg
    10141 tcggtccggg tgcggcatgg ttaaggcatt aagtttacgt gccatcgcca ttttttcgcg
    10201 tttctctaca ctttccgcag tctcttcata cagcaaggct gcctcgctgg cggggcgacg
    10261 ctgttcagta atcgccttta caatcaccgt gcgttcgtca tttccctggc gcagagtgag
    10321 cgtggcattc agctcgacga ttttgctcgg cttgctgcgc tgcccgttgt aatgcacctt
    10381 accgccttca atcatttcac gggctagcgc gcgggtttta taaaaacggg cagcccatag
    10441 ccatttatcc agtcgaacct caacagcagg tttctctttc atggcgtctc cttcacatta
    10501 gcgaggggat caggcggcgg tagtcattca gtgacggatg gcgttgatac tgtttctcgg
    10561 caatcccgga atcaggatta gtcacgccga ggcagtaacg aataccaaat tgcgcggcag
    10621 catcgagaat cgcttcgctg tcatcaataa acagcgttct ttcagctttc agacccgtag
    10681 cttcggccac cgcatgccat aaccgctgat cctctttcgg ataaccaaat gtgtgggtgg
    10741 aaagtaataa atcaaggtgt gcgtccagac ccgtatgctc aagttttacc gccaggttgt
    10801 gcggatgagc attggtgagc aaaattcgct gcttaccgct ggctttcagt gcctcaagaa
    10861 acggaatggt atcttcacgc agtacggcgc gcggtcccat ctcggtggtc attgcacaga
    10921 tatccagacc cagttgctca ctccagtaat caagacagta ccagtttagc gtatgctgta
    10981 cgtcgtgata ttgctggcgc atatattcca tcgcttcctg tggcgtaacc ccgtttttcg
    11041 cgccccatgt ttcaggcacc agcttttgcc agaaatagtt atcgaaggcg aggtcgagca
    11101 acgtgccgtc catatccagc agaacggtat ctacgtcctg ccaggcaatg ttgatatgca
    11161 tgagggaaat ctccagagtg aagcaatttg cgcgacaggg tagcataacc tgtcgcgcaa
    11221 acgtgttatt cgataaggct ttctgaaggg gtgatcagtt gcgggttcag gcagctttca
    11281 taataggcct gaatctccat catgcgagtg cgatgacgct ggtaacgacg ccaggcctgt
    11341 acgccattgt aaatggcgct acccagcatc gtcagtagca gcaatgtggt gctgagatag
    11401 cgccacaggc cggaacgatc cgggatcgga tgaaggccaa tatgctgcgt accattggcg
    11461 tcagtgaaga tttttgtgac gataccttcg gcgttaaacg gcgtatgcat cagcatttgt
    11521 gccagtttct ggaaagcgtt ccactgttct tgcggcgggt agtcgtaaag tgatgccgaa
    11581 ggccagggct gatcaacaaa atcgctgcct tcgtcgctga caatcaggaa tccgccgggg
    11641 gccggactgt ttagtgattg tgccgctcgc gccgtttcat gcgtgataaa cggcgcggtg
    11701 gaggttgcca ccaggttatc cagcgattcc gcactcaccg ggcgtaataa cacattcacg
    11761 ccatcgagct tcccggcgtt ggcgcgtttt accagcgcgt cccagtcttt actgttgccg
    11821 agattgacca gcgcattttt cagtcgcaca cagtcatctt tggcagaaca taaatccgct
    11881 gtcttcagta caatgtcgcc aaaatcatca agcaatacca tgccggattt ttgaatcgct
    11941 gaacgtaatg aggcactgac gcgagattca tcttccggtt tagggtgcag ctggcgatta
    12001 actgcttcag tcaatgccgt cgctttgttg accagttcag attctggtaa tggtaatgag
    12061 cgggcgtcat tccagatgat ctgcgagcag tcaaacggta aaaaaggtga attggttttc
    12121 gcgctccagg ttccggaagt gcgaatatta cacattcccg taccgctaat acgcaatgta
    12181 tcgcctaccc gcacgccagc gtcagccagt tgttttacgc tggtggcttc aatggtctgc
    12241 gcgcctttca tccatgagag agtgaatttc agcggcatat ccagcgggat ccagaataac
    12301 agcataaaca gcaccagcag cgagcccgca gcgataatcg tactgcgcag ccagtgctgt
    12361 aacggaaagt tttttacttc atcatgcagc gaaagatatc gcccctgacg cactacgtgg
    12421 cagtcgagat agatatcaat atcggtttgt tgatcgagat cctgagcaat gtatggctgc
    12481 cagtgtgcgg gatagaccag gtcgataata ccgagtgaaa tattgttgat ctgttcctga
    12541 tcgttttcgc caaacaatcc ccaacgacgg ggtgtaccgc gcagacaatg aatttcccgc
    12601 agggaggatt ttgccggggg cgcgaatagc ccccacagac ctgcgcccag cagcagtaat
    12661 gcgccgcctg ccagccacgg aacaaataca tccggggtaa tcaggcagaa aaagaacatc
    12721 aggaaggagg cgacgatcag caacgcttca cgcagcccgc gcggacgact cagcgcatat
    12781 tcttcatgcg tttctttgcg aatattgagt agctcgattt gctcactctc ttccccgcga
    12841 atcgacgcct gcgtggacgg aacaggttgc aaggcatagc cgcgcgtttc ctgcatgtac
    12901 tcctgtagcg tatgaccgtt gagtgaaata accaacggca gcgaatcggt atgaatcagt
    12961 tcaacggtat tctcatcgtt gatgtactgt tcccagaagg ggggcaggtg gacttctacc
    13021 gaatcgaggt agtaacgcca tttattcgga tcgtcggtag agatgccgta acgtgtgata
    13081 gcgtgtgtca gcatcatgac gttgttgctt tcggcattca gcgccagaga gatcggtgcc
    13141 gcgctagctc ccgttgggcc aggcacctgt aatacctgcg tcaggctctc aagataattt
    13201 tcaacggcgc tacgttcttc aggtgtgagt ttacgcgttt gcgcatccgc gaaggcgttg
    13261 gtccagggca gctgactccg tctggatcgc acttttatca gccatcccgc aagtagtgag
    13321 caggccagca aagcagctaa aaaaatcaca atggtgctca tgctttcccc atcttactta
    13381 gtctgtcagg cgtggtcagt cgcaccagac acaatacatg attctgcggt tggctatcgg
    13441 caagcgtgga gtttaacttg agcatcatta agcacatcac agtaaccgct gatcatagca
    13501 aaccctctga aggcggaata tcagcaaatt cctagagtta tttcaaccta ttgactttta
    13561 atttgatttg aggacgatta tttattaagt tacataaatg taaataacat gcaattcaca
    13621 ttgccgaaag cgtgtccgat atcgcacaat aaccgctgat ttcacagcat agatcccacg
    13681 atgagcaaat cattacaaaa acccaccatt ctgaatgttg aaactgtagc ccgttcccga
    13741 ctgtttaccg tcgagagcgt ggatctggag ttcagcaatg gcgtgcggcg tgtttatgaa
    13801 cgaatgcgtc caaccaaccg ggaagcagtg atgattgtgc cgattgtgga cgatcacctg
    13861 atcctgatcc gcgaatacgc agtgggaact gaatcctacg aattaggttt ttcgaaagga
    13921 ttaattgatc cgggtgaaag cgtctacgaa gccgctaacc gcgagctaaa agaagaggtt
    13981 ggatttggtg cgaacgatct gacttttttg aagaagctca gcatggcacc gtcttacttt
    14041 tccagcaaaa tgaatatcgt ggtcgcgcaa gatctctacc cggaatcgct ggaaggcgat
    14101 gagccagagc cgctaccaca ggtgcgctgg ccgctggcgc atatgatgga tttgctggaa
    14161 gaccctgact tcaacgaagc gcgtaatgtc agcgcgctgt tcctcgtgcg cgaatggttg
    14221 aaagggcagg ggcgagtgta aatgcgtgaa gtttgcctga tgcacaacgc ttatcaggct
    14281 tatggggttc ctgcaatata ttgatttttc acgattttgt tagccgggta acgcggtcac
    14341 ccgcatccag cataaacaaa tgcactttgt cagccaactg aaaaggcgcc gaagcgcctt
    14401 tttaatcaga acaattcctg tgcctcacca ttatcgataa tggtcgttcc cacctcgtgc
    14461 accgcctgtt gtgtcggctg cgtaccttcg atgaaatact cttcgcggct gttgccacca
    14521 ttagctaact gcccggtgct gcgatcgata ttcaccgtca caatacccgg tggcggcgtc
    14581 agcggctgct ccggcacacc ttcaagaacg gctttcatat aagcgtccca tgcaggctgg
    14641 gcactcttgg ctccgccttc gtaacctgag atctgatctt taatcgctcc ggaagccgtt
    14701 gtatgaccga gattacgacg gtgatcatca aagccaatcc agaccgaggt cacaacgccc
    14761 ggaccgtaac ccgagaacca cgcatctttc gaactgttag tggtaccggt tttcccgccg
    14821 atatcgcgac gctgcaaatc acgacctgca cgccagccag taccctgcca gcctggctca
    14881 ccaaagatat tggtgttcaa agcactctta atcaggaatg ccagcggagt gttgatgacg
    14941 tgcggtgcgt actcctgcgc gccagtcttc gccactaacg cctgatttgc ctgctccagc
    15001 tgcggcattg gtacagaaac attctgctgc tcgcgggaga tagcgacatc ttcaacatcg
    15061 ttattttcca gcacgttcga tttctgcgta tcaccgtaaa tcaccggaat atcgcattcc
    15121 gggcaggcta ctttcggttt cgcttcgaaa atcacgccgc cctgatcgtt ttcaattttg
    15181 ctgataaacc acgggtccac caggaagccg ccgttcgcca tgaccgcgta gccgcgcgcc
    15241 acctgcattg gggtgaagga cgctgaaccc agcgccagcg attcggtgtg gacaatgttt
    15301 tgcgccggga agccgaagcg ttgcagatat tctgcagcgt agtcgacgcc catagcccgc
    15361 attgcacgta ccatcaccac gtttttcgac tgacccagcc cctgacgtaa gcgaattgga
    15421 ccagcatatt gtggcggcga gtttttcggc tgccagtcag aaccggcacc tgcgtcccag
    15481 cgagaaattg gcacatcgtt caacatactt gccagcgtca gacctttatc catcgccgcg
    15541 gtgtagagga acggtttgat gttggaaccc acctgacgca gtgcctgggt ggcgcggtta
    15601 aacttgctct gattgaaatc aaagccaccg accagcgcca taacggcacc gttttgcgga
    15661 ttgatcgaca ccagcgccga gttcacttcc ggcacttgtg ccagccacca tgcatcgcca
    15721 acctgacgaa cccagatttg ctgacccgtt tgcagaacat cggtcacttt acgcggcgtc
    15781 ggtccctgct gagtatccga acggtaagga cgcgcccagc gaacgccttc catactcaat
    15841 gcgacggtcg acccgtccgc cagcatcgcc gtcgctccct gaggattggc gctggtgact
    15901 gcggcaggca gcagcggacc ataggtcggc agcgccttca gcgtatcggt aatcttgttg
    15961 ttatcccacg ccgactcgcc cactttccac agcacatttg ccgggccgcg atagccgtgg
    16021 cgcatgtcgt agtccagcac gttattacgt accgcctgct gtgcggcctg ctgcactttg
    16081 cgggtgatgg tggtgtaaat gcgataaccg tcttcatagg cactttcgcc ataacggtta
    16141 tacatctcct ggcgcaccat ttcgctcagg tacggcgcag agaaagcaat ctccggcgcg
    16201 tgatagttag cgttaatcgc ctcagtgcgt gtctgatcga actgttgttg ggtgatatac
    16261 ccttcatcca gcatccgcga cagtacgacg ttacgccgcg cgacggcacg atccattgag
    16321 tagagcggat taaaggtgga aggcgctttc ggcagcccgg ctatcacagc catttcgttc
    16381 agcgtcagtt ggtcgaccgt tttaccgaaa tagacttgtg ccgcagcacc gacaccatag
    16441 gcgcggtaac cgaggtaaat cttattcaga taaagctcga ggatctcgtc tttcgtcagc
    16501 aactgttcaa tgcgaatcgc gaggaagact tccttaatct tacgcatcag cgtgcgttct
    16561 ggactgagga agaagtttct cgccagctgc tgggtaatgg tactcgcccc ttgtgacgcg
    16621 tggccggaga acagcgccac acttgccgca cggaagatcc ccaccgggtc aacgccgtga
    16681 tgctcgtaga agcggctgtc ttccgtcgcg ataaaggctt tcaccatctc cggcgggatt
    16741 tgatccaacg taaccggaat acgacgtttc tcaccgtatt gagcaatcag ctcgccatcg
    16801 gcgctgtaaa tctgcatcgg aatttgcagg cgaacatctt ttaatgtcgc cacatccggc
    16861 agttgtggct cgatgtagcg gtataggcca taaatcgagc ctgctcccag cagaatgcaa
    16921 cagactgcaa ggatcaaaaa atactttacg aacttcactg gaaatttccc atttagtttc
    16981 atttgggcag tttataaaca aacgcgcgtt agtataaagg caagccagac gcattgatat
    17041 acccgtcaga gtgacgggtg ataaggagat catcacaatg gcatttaaga tctggcaaat
    17101 tggtttgcat ttacaacagc aagaagcggt agcggttgcg atcgtgcggg gcgcaaaaga
    17161 atgctttttg caacgctggt ggcggttgcc gctggagaac gacattatca aagatgggcg
    17221 gattgttgat gcgcagcagc tggctaaaac gttgttacct tggagtcgcg aactgccgca
    17281 gcgtcatcac attatgttgg cgtttcccgc cagtcgcaca ttacagcggt catttccgcg
    17341 cccgtcgatg tcccttggtg agcgggagca aacggcctgg ctgtcaggga cgatggcccg
    17401 cgagctggat atggatccgg actccctgcg cttcgattac agcgaagact cactcagtcc
    17461 cgcctataac gtgactgccg cgcaaagcaa agagctggca acgctgctta ctctggcaga
    17521 aaggttgcgt gttcatgtta gtgcgatcac cccggatgcc agtgcattac agcgattcct
    17581 gcctttttta ccttctcatc agcaatgtct ggcctggcgt gataacgaac agtggctgtg
    17641 ggcgacgcgc tatagctggg ggcgcaaact ggcggtgggg atgactagcg cgaaggagct
    17701 ggcggcagcg ttattcgttg atcccgacag cgtcgcgata tgtggcgaag gcggatttga
    17761 cccctgggag gccgtttctg ttcgtcagcc gctgctaccg ccgcctggtg gagactttgc
    17821 catcgcgctg gggctggcgc ttgggaaggc gtactgatga acccgccaat taattttttg
    17881 ccctggcgac agcaacgccg gaccgctttt ctgcgtttct ggttgctgat gttcgttgcg
    17941 cctctgctgc tggccgtcgg gataacgcta atactgcgtc tgacaggcag cgccgaagcg
    18001 cgcatagacg cagttttgct tcaggcggaa caacaactcg cccgcagctt acagataacg
    18061 aagccacgtt tgctggagca acagcagtta cgcaaacagc gttcgcaacg gcagcgccag
    18121 cgacaattta cccgcgactg gcaatctgcg ctggaagcac tggcggcgct tttacccgag
    18181 cacgcctggc tgacaacgat aagctggcag cagggaacgc tggagatcaa ggggctgaca
    18241 acaagcatta ccgcgttaaa cgcactagaa acgtcactcc gccaggatgc gtcttttcat
    18301 ctcaatcagc ggggagccac gcagcaggat gcgcagggac gctggcaatt tgagtatcag
    18361 ttaacaagga aggttagcga tgaacatgtt ctttgactgg tggttcgcca catcaccccg
    18421 cctccgccag ttttgctggg cattctggtt gctgatgtta gttacgctca tttttctgtc
    18481 atcgacacac catgaagagc gcgacgcatt aattcgatta cgggcaagtc atcaccagca
    18541 gtgggccaca ctgtatcgcc tggttgacac cactcccttc agcgaagaaa aaacgctgcc
    18601 cttttcgcca ctggattttc agttgcccgg cgcgcaactg gtttcctggc atccatccgc
    18661 gcagggaggt gagttggcgt tgaaaacgct gtgggaagca gtgccgtcgg cattcacacg
    18721 gctggcagag cgcaacgtca gcgtgagccg tttttcgtta agcgtggaag gtgatgatct
    18781 tttgttcacg ctacaactgg agacgccgca tgagggttaa acgctggttg ttggcaggta
    18841 ttgcattgtg ccttttaacc ggtatgcgtg acccttttaa accgccggaa gatctatgcc
    18901 ggattagcga acttagccag tggcgctatc aggggatggt agggcgaggc gagcgcatca
    18961 tcggtgtaat aaaagacggg caaaagaaat ggcgacgggt gcagcaaaac gatgtgctgg
    19021 aaaacggctg gacaatttta cagctgacgc cagacgtact aacgctgggt accgggacaa
    19081 actgcgaacc gccacaatgg ttgtggcaac ggcaaggaga tacaaatgaa gcaatggata
    19141 gccgcactac tgttgatgtt gatacccggc gtacaggcgg caaagccgca aaaagtgacg
    19201 ctgatggtgg atgacgttcc ggtagctcag gtgttgcagg cgctggctga acaggagaag
    19261 ttgaacctgg tcgtgtcgcc agacgtcagc ggtacggtgt cgttacatct aacagatgtt
    19321 ccctggaagc aggcactaca aactgtagtg aaaagcgccg gactgataac gcggcaggaa
    19381 ggcaacattc tctcagtgca ttccattgcc tggcagaata acaatatcgc ccgccaggag
    19441 gcggagcagg cgcgggcgca ggcaaatctg ccgctggaaa atcgcagtat aaccctgcaa
    19501 tacgccgacg cgggagaact ggcgaaagcg ggggagaagc tactgagtgc caaagggagt
    19561 atgaccgtcg ataaacgcac caatcgcctt ttgctacgag ataacaaaac ggcgttaagc
    19621 gcgcttgaac agtgggtagc gcaaatggat ctgccggtcg ggcaggttga gctgtcggcg
    19681 catattgtca ccattaatga aaaaagtttg cgtgagttag gcgtgaaatg gacgctggcc
    19741 gatgcgcaac acgctggtgg cgttgggcaa gtcaccacgc ttggtagcga cctctccgta
    19801 gcgacggcga caacgcatgt cggttttaac attgggcgca tcaacggacg cttgctggat
    19861 cttgagcttt ccgcgctcga acaaaaacag cagctggata ttatcgccag tccgcgtctg
    19921 ctggcctcac atcttcagcc tgccagcatt aaacagggga gcgaaattcc atatcaggtt
    19981 tccagcgggg aaagtggcgc gacgtcggtg gaatttaaag aggccgtcct ggggatagag
    20041 gtcacgccca cggtgttaca aaaaggtcgc atccggctga aattacacat cagccagaac
    20101 gttccggggc aggtgctaca gcaggccgat ggcgaagtgc tggcgattga taagcaggag
    20161 atcgaaacgc aggtcgaggt caaaagcgga gaaacgttgg cgctgggcgg catttttacc
    20221 cgtaaaaata aatcgggtca ggatagcgta ccgttgcttg gcgacattcc ctggttcggg
    20281 caattatttc gtcatgacgg aaaagaatat gaacgacgcg agttagtggt gtttatcacg
    20341 ccacgactgg tttccagtga gtaaacagcc gtaaaagcgg taatgttttt atgctgaacg
    20401 tgtttcatct atttgacgcg cgcaggtatt tagcatacaa ggagtaccga tttgagagtt
    20461 ggcgctcttc gctgcctgcg ttccatgatg atgatttatc attcaggcgg cattttgctg
    20521 tcttttttac gctaatctta cccggtgatt tatcgccaga gcggtggtag caaggcagcg
    20581 cgcttgcagc gaccagatat gcagagggat gggtgattta ttcagttgcc aaacccgctg
    20641 gagtattgag ataattttca gtctgactct cgcaatatct tatgaggttt cagttcatgt
    20701 cctgcggcgc tctctgagcg aagcgggttt atcattaacg aatagtctta gtagtaccga
    20761 aaaaatggca gagaaacgca atatctttct ggttgggcct atgggtgccg gaaaaagcac
    20821 tattgggcgc cagttagctc aacaactcaa tatggaattt tacgattccg atcaagagat
    20881 tgagaaacga accggagctg atgtgggctg ggttttcgat ttagaaggcg aagaaggctt
    20941 ccgcgatcgc gaagaaaagg tcatcaatga gttgaccgag aaacagggta ttgtgctggc
    21001 tactggcggc ggctctgtga aatcccgtga aacgcgtaac cgtctttccg ctcgtggcgt
    21061 tgtcgtttat cttgaaacga ccatcgaaaa gcaacttgca cgcacgcagc gtgataaaaa
    21121 acgcccgttg ctgcacgttg aaacaccgcc gcgtgaagtt ctggaagcgt tggccaatga
    21181 acgcaatccg ctgtatgaag agattgccga cgtgaccatt cgtactgatg atcaaagcgc
    21241 taaagtggtt gcaaaccaga ttattcacat gctggaaagc aactaattct ggctttatat
    21301 acactcgtct gcgggtacag taattaaggt ggatgtcgcg ttatggagag gattgtcgtt
    21361 actctcgggg aacgtagtta cccaattacc atcgcatctg gtttgtttaa tgaaccagct
    21421 tcattcttac cgctgaaatc gggcgagcag gtcatgttgg tcaccaacga aaccctggct
    21481 cctctgtatc tcgataaggt ccgcggcgta cttgaacagg cgggtgttaa cgtcgatagc
    21541 gttatcctcc ctgacggcga gcagtataaa agcctggctg tactcgatac cgtctttacg
    21601 gcgttgttac aaaagccgca tggtcgcgat actacgctgg tggcgcttgg cggcggcgta
    21661 gtgggcgatc tgaccggctt cgcggcggcg agttatcagc gcggtgttcg tttcattcaa
    21721 gtcccgacga cgttactgtc gcaggtcgat tcctccgttg gcggcaaaac tgcggtcaac
    21781 catcccctcg gtaaaaacat gattggcgcg ttctaccagc ctgcttcagt ggtggtggat
    21841 ctcgactgtc tgaaaacgct tcccccgcgt gagttagcgt cggggctggc agaagtcatc
    21901 aaatacggca ttattcttga cggtgcgttt tttaactggc tggaagagaa tctggatgcg
    21961 ttgttgcgtc tggacggtcc ggcaatggcg tactgtattc gccgttgttg tgaactgaag
    22021 gcagaagttg tcgccgccga cgagcgcgaa accgggttac gtgctttact gaatctggga
    22081 cacacctttg gtcatgccat tgaagctgaa atggggtatg gcaattggtt acatggtgaa
    22141 gcggtcgctg cgggtatggt gatggcggcg cggacgtcgg aacgtctcgg gcagtttagt
    22201 tctgccgaaa cgcagcgtat tataaccctg ctcaagcggg ctgggttacc ggtcaatggg
    22261 ccgcgcgaaa tgtccgcgca ggcgtattta ccgcatatgc tgcgtgacaa gaaagtcctt
    22321 gcgggagaga tacgcttaat tcttccgttg gcaattggta agagtgaagt tcgcagcggc
    22381 gtttcgcacg agcttgttct taacgccatt gccgattgtc aatcagcgta acaacaagaa
    22441 aggtcaggcc gcttatcaag cggtctatta gcttcaggtt aattgcaacg tggtaagcat
    22501 taacctttta gtggggtgtt aaatggatga attcaaacca gaagacgagc tgaaacccga
    22561 tcccagcgat cgtcgtactg gtcgttctcg tcaatcttct gaacgttctg agcgtactga
    22621 acgtggcgaa ccgcagatca attttgatga tattgaactt gatgacactg acgatcgccg
    22681 tccgactcgt gcgcaaaaag agcgcaatga ggaaccggaa atcgaagaag aaattgacga
    22741 atccgaagat gaaaccgtgg atgaagagcg cgtagagcgt cgtccgcgta agcgcaaaaa
    22801 agcagccagt aaacccgctt ctcgtcagta tatgatgatg ggcgtcggca ttctggttct
    22861 actgctgttg atcatcggta tcggttctgc gctaaaagcc ccctcgacct cttccagcga
    22921 tcaaaccgcg tctggcgata agagtattga tcttgcaggc aatgcgaccg atcaggcgaa
    22981 tggggtgcag ccagcgccgg gaaccacgtc tgcggaaaat actcagcagg atgtttctct
    23041 gccaccgatc tcttctacgc cgactcaagg gcaaaccccg gcggcaacgg atggtcaaca
    23101 acgtgttgaa gtgcagggtg acctgaacaa tgcgctgact cagccacaaa atcagcaaca
    23161 gttgaacaat gtggcggtca attccacatt gccgaccgaa cccgcgactg tcgcgcctgt
    23221 tcgcaatggc aatgcatcgc gtgacacggc gaaaacgcaa accgctgaac gtccgtccac
    23281 tacgcgccca gctcgtcagc aggcggtgat tgaaccgaaa aaaccgcaag caaccgtgaa
    23341 aacggagccg aagccggtag cacagacgcc gaagcgtact gaaccagctg cccctgtggc
    23401 gagcacgaag gcaccggctg cgacttctac gccagcacca aaagagacgg cgactacggc
    23461 tccagtacag acggcatccc cggcgcaaac cacggcaaca ccagccgctg gagggaagac
    23521 cgcaggtaat gttggttcgt tgaaatcggc accgtccagc cattacactc tgcagctgag
    23581 cagttcctct aactacgaca acctgaacgg ttgggcgaag aaagagaatc tgaaaaacta
    23641 cgttgtctat gaaacgacgc gtaatggtca gccgtggtat gtcctggttt ctggcgtgta
    23701 cgcttcgaaa gaagaggcga aaaaagcggt atctacattg ccagcagatg ttcaggccaa
    23761 aaacccgtgg gcgaaaccgc tgcgtcaggt acaggccgat ctgaagtaat caaggttatc
    23821 tcccgcaatg gtttatcgtt gcgggagctg cctgaagcgc tggatgctgt cggagctttc
    23881 tccacagccg gagaaggtgt aattagttag tcagcatgaa gaaaaatcgc gcttttttga
    23941 agtgggcagg gggcaagtat cccctgcttg atgatattaa acggcatttg cccaagggcg
    24001 aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc gacttttctc
    24061 gttatatcct tgccgatatc aatagcgacc tgatcagtct ctataacatt gtgaagatgc
    24121 gtactgatga gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca aattgcgccg
    24181 aggtttacta tcagttccgc gaagagttca acaaaagcca ggatccgttc cgtcgggcgg
    24241 tactgttttt atatttgaac cgctacggtt acaacggcct gtgtcgttac aatctgcgcg
    24301 gtgagtttaa cgtgccgttt ggccgctaca aaaaacccta tttcccggaa gcagagttgt
    24361 atcacttcgc tgaaaaagcg cagaatgcct ttttctattg tgagtcttac gccgatagca
    24421 tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc gccttatgca ccgctgtctg
    24481 cgaccgccaa ctttacggcg tatcacacaa acagttttac gcttgaacaa caagcgcatc
    24541 tggcggagat cgccgaaggt ctggttgagc gccatattcc agtgctgatc tccaatcacg
    24601 atacgatgtt aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc aaagttcgac
    24661 gcagtataag cagcaacggc ggcacacgta aaaaggtgga cgaactgctg gctttgtaca
    24721 aaccaggagt cgtttcaccc gcgaaaaaat aattctcaag gagaagcgga tgaaacagta
    24781 tttgattgcc ccctcaattc tgtcggctga ttttgcccgc ctgggtgaag ataccgcaaa
    24841 agccctggca gctggcgctg atgtcgtgca ttttgacgtc atggataacc actatgttcc
    24901 caatctgacg attgggccaa tggtgctgaa atccttgcgt aactatggca ttaccgcccc
    24961 tatcgatgta catctgatgg tgaaacccgt cgatcgcatt gtgcctgatt tcgctgccgc
    25021 tggtgccagc atcattacct ttcatccaga agcctccgag catgttgacc gcacgctgca
    25081 actgattaaa gaaaatggct gtaaagcggg tctggtattt aacccggcga cacctctgag
    25141 ctatctggat tacgtgatgg ataagctgga tgtgatcctg ctgatgtctg ttaacccagg
    25201 ttttggcggt cagtcgttca ttccacaaac actggataaa ctgcgcgaag tacgtcgtcg
    25261 tatcgacgag tctggttttg acattcgact ggaagtggac ggtggcgtga aggtgaacaa
    25321 cattggcgaa atcgctgcgg cgggcgcgga tatgttcgtc gccggttcgg caatcttcga
    25381 ccagccagac tacaaaaaag tcattgatga aatgcgcagt gaactggcaa aggtaagtca
    25441 tgaataagtt tgaagatatt cgcggcgtcg cttttgatct cgacggtacg ctggttgaca
    25501 gtgctcccgg tcttgctgct gcggtagata tggcgctgta tgcgctggag ttacccgtcg
    25561 caggtgaaga acgcgttatt acctggattg gtaacggcgc agatgttctg atggagcgtg
    25621 cattggcctg ggcgcgtcag gaacgtgcga ctctgcgtaa aacaatgggt aaaccgcccg
    25681 ttgatgacga cattccggct gaagaacagg tacgtattct gcgtaaactg ttcgatcgct
    25741 actatagcga ggttgccgaa gaggggacgt ttttgttccc gcacgttgcc gatacgttgg
    25801 gcgcgttgca ggctaaaggc ctgccgctag gcctggtcac caacaaaccg acgccgttcg
    25861 tcgcgccact gctcgaagcc ttagatattg ccaaatactt tagcgtggtt atcggcggcg
    25921 atgatgtgca aaacaaaaaa ccgcatccgg acccgctgtt actggtggct gagcggatgg
    25981 gaattgcccc acaacagatg ctgtttgtcg gcgactcacg caatgatatt caggcggcaa
    26041 aagcggcagg ttgcccatca gttggcttaa cctacggata taactacggc gaggctatcg
    26101 atctcagcca gcctgatgta atttatcagt ctataaatga ccttctgccc gcattagggc
    26161 ttccgcatag cgaaaatcag gaatcgaaaa atgactaagc ccatcgtttt tagtggcgca
    26221 cagccctcag gtgaattgac cattggtaac tacatgggtg cgctgcgtca gtgggtaaac
    26281 atgcaggatg actaccattg catttactgt atcgttgacc aacacgcgat caccgtgcgc
    26341 caggatgcac agaagctgcg taaagcgacg ctggatacgc tggccttgta tctggcttgt
    26401 ggtatcgatc ctgagaaaag caccattttt gttcagtccc acgtaccaga acatgcgcag
    26461 ttaggctggg cactgaactg ctatacctac ttcggcgaac tgagccgcat gacccagttt
    26521 aaagataaat ctgcgcgtta tgccgagaac atcaacgctg gtctgtttga ctatccggtg
    26581 ctgatggctg cggacatcct gctgtatcaa actaatctgg taccggtggg tgaagaccag
    26641 aaacagcacc tggaactgag tcgcgatatc gcccagcgtt tcaacgcgct gtatggcgag
    26701 atctttaagg tgccggagcc gtttattccg aaatctggcg cgcgcgtaat gtcgctgctg
    26761 gagccgacca agaagatgtc caagtctgac gataaccgca ataacgttat cggcctgctg
    26821 gaagatccga aatcggtagt gaagaaaatc aaacgcgcgg tcactgactc cgacgagccg
    26881 ccggtagttc gctacgatgt gcagaacaaa gcgggcgttt ccaacctgct ggatatcctt
    26941 tctgcggtaa cgggccagag catcccagaa ctggaaaaac agttcgaagg caagatgtat
    27001 ggccatctga aaggtgaagt ggctgatgcc gtttccggta tgctgactga attgcaggaa
    27061 cgctatcacc gtttccgcaa cgatgaagcc ttcctgcaac aggtgatgaa agacggcgca
    27121 gaaaaagcca gtgtacacgc ttcccgtacg ctgaaagcgg tgtacgaagc gattggtttt
    27181 gtggcgaagc cgtaatatca aaacgctcgg acggtcccct cgcccctttg gggagagggt
    27241 tagggtgagg ggagcaggca agcgccagtg ggaattaccc tttgctcgca ttgtcgaata
    27301 aatcacttcg tcatcactct gaaaccgggg aaacccggtt tttttatcct catttgcaat
    27361 aatccgaaaa aatgtgaagc gcctcgccgt ttccacatct cacagttgcc acttatcttc
    27421 attttaatga agatataaac attcatttta ttgaaaatat attatgcgtc gaacgtttat
    27481 caaaaaagaa ggcgtcgtca tcacgacgct ggcccgttat ttgttgggtg aaaaatgcgg
    27541 taatcgactg aaaaccatag atgagctggc aactgaatgc cgttcatcgg ttggcctgac
    27601 gcaggccgcg ttgaaaacgt tggaatcaag tggagcgata cggattgaac gccgtgggcg
    27661 caatggcagt tatctggtcg aaatggataa caaagcgctg ctgagtcatg tggatatcaa
    27721 taacgttgtg tgtgccatgc ctttgcccta tacccgcttg tacgaaggtt tagcaagcgg
    27781 gctgaaagcc cagtttgatg gcattccttt ttattatgcg catatgcgtg gcgcggatat
    27841 tcgtgtggag tgtttgctta acggtgtcta tgacatggca gtagtttccc gcctggcggc
    27901 ggaaagttat ctcagccaaa acaatttgtg catcgccctc gagcttggcc cgcatacgta
    27961 tgttggtgaa catcaactga tatgccgcaa aggtgagtct ggaaatgtta agcgagtagg
    28021 tctcgatagt cgttcggcgg atcagaaaat catgaccgat gtcttttttg gcgatagtga
    28081 tgtggaacgt gtcgatctct cttatcacga gagtttacaa cgcattgtta aaggcgatgt
    28141 cgatgcggtt atctggaacg tggtggcgga aaacgaactg accatgctgg gattagaggc
    28201 gacgccgctc acagacgatc cgcgattttt acaggccacc gaagccgtgg tcctgacgcg
    28261 agtcgatgat tacccaatgc aacaactgct gcgtgccgtt gtagataaac acgccctgct
    28321 cgcccatcaa caacgggtag tgagtgggga acaggaaccg agttattaaa cgaaaggcat
    28381 ctgatatgga aaccagactc aacctgcttt gcgaggcagg cgttattgat aaggacatct
    28441 gcaaaggcat gatgcaggtc gtcaacgtac tggaaacaga gtgccatctg ccggtgcgca
    28501 gtgagcaagg aacgatggcg atgacacata tggcgagtgc actgatgcgc agtcgccgtg
    28561 gtgaagaaat agagccgctg gataacgaat tgctggcaga gctggcgcaa tccagccact
    28621 ggcaagccgt tgtgcaattg catcaggtgt tgttgaagga attcgcactg gaagttaatc
    28681 cgtgtgaaga aggctatttg ctggccaacc tttatggatt atggatggct gctaacgaag
    28741 aggtttgaag ctcccgacaa atccacgtcg cacaacctaa atattcaaaa aaatgaatat
    28801 ttaattcaag gaacagaaac gaggcaacaa gatgtttgta gaagcattga aacgccagaa
    28861 cccggcgctg atttccgccg cactaagttt gtggcagcag ggcaagatcg ccccggacag
    28921 ctgggtgatc gacgtggatc aggtactgga aaacggtaag cgactgattg agacggcgcg
    28981 gctttacggc attgaactgt atctgatgac caagcagttt ggccgtaacc catggctggc
    29041 ggaaaaattg ctggcattag gttacagcgg cattgtggcg gtggattaca aagaggcgcg
    29101 agtcatgcgc cgcgctggtt tgtcggtggc gcatcagggg catctggtac aaatcccttg
    29161 tcatcaggtt gctgacgccg ttgaacaggg caccgacgtc atcaccgtgt ttactctcga
    29221 caaagcgcgg gaagtttctg cggcagcggt gaaggccgga cgtgtgcagt ttgtgctgct
    29281 caaagtttat agcgacgatg attttcttta tccggggcag gagagcggtt ttgtacagca
    29341 ttcgctgcac gaggtggttg ctgaaattaa gaaactgccg ggtctgcatt tagccggact
    29401 tacccatttc ccttgcctgc tttgggatga ggctgccgga aaagttttgc cgacaccgaa
    29461 tcttcacacg ctgatacagg cacgggatca actggcgaaa tctggtattg cacttgagca
    29521 actgaacgcg ccttcagcga ccagctgcac ttcgctgcca ttactggcgc aatacggtgt
    29581 gactcatgcc gaacccggtc atgcgctgac gggcactatt ccggcaaacc agcagggcga
    29641 tcagtctgaa cgtatcgcga tgctctggtt gagtgaaatc tcccatcatt tccgtggcga
    29701 cagctactgc tacggcggcg gttactaccg tcgtggtcat gcgcaacatg cgttggtgtt
    29761 tacgccagaa aatcaaaaga ttactgaaac caatctcaaa actgtggatg acagcagtat
    29821 cgactacacc ctgtcgctgg caggcgagtt tccggtaagc agtgcagtgg tgctctgttt
    29881 ccgcacgcag atttttgtca cccgtagcga tgtggtgctg gtgtccggta ttcaccgtgg
    29941 cgaaccgaaa atcgttggtc gttatgacag tcttggaaac tctctggggg cgtaatggcg
    30001 cgatttgtgg tgttagtgat tgatagcttt ggcgtagggg caatgaaaga tgtcacgctg
    30061 gtgcgtccgc aagatgcggg agcgaataca tgtggtcaca tcctgagcca gttgccgcat
    30121 ttgcagctac caacgctgga gaagctgggg ctaatcaacg cattgggtta tgcgccaggc
    30181 gatatgcagc cgtcagattc cgcaacctgg ggcgtggcag agctgcaaca tgaaggtggc
    30241 gataccttta tggggcatca ggaaatttta ggcacgcgcc cgttaccgcc gctgcggatg
    30301 cctttttgcg atgtgattga ccgtgttgag caggcattag tatccgccgg ttggcaggtg
    30361 gagcgccgtg gcgatgaact gcaatttctg tgggtcaatc aggcggttgc gattggcgat
    30421 aatctcgagg cggatttagg ccaagtctat aacattaccg ccaatctctc tgtgatctct
    30481 tttgacgacg caatcaaaat tggtcgtatc gtgcgtgagc aggtacaggt cggtcgggtc
    30541 attacatttg gtggcctgtt aaccgacagt caacgcattc tcgatgccgc agaaagcaaa
    30601 gaagggcgct ttattggtat caatgcgccg cgttctggcg cttatgacaa cggcttccag
    30661 gtcgtgcata tgggctacgg tgtcgataaa aaagtgcagg tgccgcaaaa actgtatgag
    30721 gcgggcgtgc caaccgtgct ggtgggtaag gtggcagata tcgtcagcaa tccttatggc
    30781 gtgagctggc aaaacctggt ggatagccag cggattatgg atatcaccct cgacgaattt
    30841 aacacccatc cgacggcgtt tatttgcacc aatatccagg aaaccgacct cgctggtcat
    30901 gcagaagacg tcgcacgtta tgccgaacgt ttgcaggtcg ttgaccgtaa ccttgcccgg
    30961 cttgttgatg cgatgcagcc agatgattgc ctggtcgtga tggcggatca cggcaacgat
    31021 ccgaccattg gtcacagcca ccatacccgc gaagtggtgc cgctgctggt ttatcagcaa
    31081 ggcctggctg ctacgcagct cggtgtgcgc accacgcttt ctgatgtggg agctaccgtg
    31141 tgtgaatttt tccgtgcgcc accgccacaa aatggtcgct cttttctttc ctccctccgg
    31201 tttgcaggag acaccctatg agttttgatc cgacgggtta caccctggcc catgagcatc
    31261 tgcatattga tctctccggc tttaaaaaca acgtggactg ccgccttgat cagtatgcgt
    31321 tcatttgcca ggagatgaac gacctgatga cccggggtgt acgtaatgtg attgagatga
    31381 ccaaccgtta catggggcgc aatgcgcaat ttatgctcgg tgtaatgcgc gaaaccggga
    31441 tcaacgtggt ggcctgtact ggttattacc aggatgcgtt tttcccggaa catgtggtga
    31501 cccgcagcgt gcaggaactg gcgcaggaga tggtcgatga aattgaacag ggtatcgatg
    31561 gcacggagct gaaagccggg ctcatcgcgg agatcggctc cagcgaagga aagattacgc
    31621 cgctggaaga gaaggtattt attgccgctg cgctggcgca taaccagacc ggacgcccga
    31681 tctccacgca tacgtcgttc agcaccatgg ggctggaaca actggctctg ttgcaagccc
    31741 acggagttga tctttcgcgc gtcaccgttg gtcactgcga tctgaaagac aacctcgaca
    31801 acattttgaa gatgatcgat ctcggcgcgt acgtgcagtt cgacaccatt ggtaagaaca
    31861 gttactaccc ggacgaaaag cgcattgcga tgctccacgc gctacgtgac cgtgggttgc
    31921 tgaaccgcgt catgctgtcg atggatatta cgcgccgctc ccatttaaaa gccaatggtg
    31981 gttatggcta tgactattta ttgaccacct ttattccgca attgcgccag tcaggattca
    32041 gtcaggccga tgtggatgtg atgttacgtg aaactccctc tcaatttttc caataaggac
    32101 agactcatga aaaagattgg cgttgcaggc ttacagcgtg agcagattaa aaaaaccatt
    32161 gaagcgacgg ctcctggcag ttttgaagtt ttcattcaca acgacatgga agcggcaatg
    32221 aaagtgaaat ccgggcaact ggattactac atcggtgcgt gtaataccgg tgcgggcgcg
    32281 gcattgtcga ttgccatcgc ggtgattggc tataacaaaa gttgcaccat tgccaaacca
    32341 ggcattaaag cgaaagacga gcatatcgcc aaaatgatcg ctgaaggaaa agtggcgttt
    32401 ggcctttccg ttgagcacgt cgaacacgcg attccgatgc tgattaacca tctgaaataa
    32461 aaggcacgac tatggatctg tatattcaga ttatcgtggt ggcgtgcctg acgggtatga
    32521 catcgcttct ggcgcatcgc tcggcggctg tttttcatga cggcatccgc ccgatcctgc
    32581 cgcaactgat tgaaggctat atgaaccgtc gcgaggcggg gagtatcgct tttggtctga
    32641 gcattggttt tgtggcctcg gtggggatct cttttaccct gaaaaccggg ctgctcaacg
    32701 catggttact ctttcttcct accgatatcc tcggcgtact ggcgataaac agcctgatgg
    32761 cgtttggtct tggcgctatc tggggcgtgt tgatccttac ttgcctgttg ccagtaaacc
    32821 agctgctgac cgcgctgccg gtggatgtat taggtagcct cggggaatta agctcgccgg
    32881 tagtttctgc ttttgcactg ttcccgctgg tggcgatttt ctaccagttt ggctggaagc
    32941 aaagtctggt cgccgccgtg gttgttctga tgacccgtgt ggtagtcgtg cgctatttcc
    33001 cacatcttaa ccctgaatcc atcgaaatct ttattggcat ggtgatgctg ctggggatcg
    33061 cgataactca cgacctgcgt catcgtgatg aaaatgatat tgatgccagc gggctttcgg
    33121 tgtttgaaga acgcacgtcg cggattatca aaaacttacc ctatatcgcc atcgtgggag
    33181 cattgattgc cgccgttgcc agcatgaaga ttttcgccgg cagtgaagtg tcgatcttca
    33241 ctctggagaa agcgtactcc gcaggcgtaa cgccggaaca atcgcaaacg ctgatcaacc
    33301 aggcggcact ggcagaattt atgcgcgggc tgggttttgt accgttgatt gccaccaccg
    33361 ccttagcaac gggtgtgtat gcagttgcgg gctttacctt tgtttatgcg gtgggctatc
    33421 tctcgccgaa tccgatggtt gcagcggtat taggcgcagt ggttatttcg gcggaagtct
    33481 tgctgcttcg ttctatcggc aaatggctgg gacgctaccc gtcggtgcgt aatgcgtcgg
    33541 ataacatccg taacgccatg aatatgctga tggaagtggc gctgctggtc ggttcgattt
    33601 tcgcagcaat taagatggcg ggttataccg gattctctat cgcggttgcc atttacttcc
    33661 tcaacgaatc cctgggccgt ccggtacaga aaatggcggc accggtcgtg gcagtaatga
    33721 tcaccggtat tctgctgaat gttctttact ggcttggcct gttcgttccg gcttaaggaa
    33781 ggcacctatg aagacgtttc ctctgcaaag cctgacgctt attgaggcgc agcaaaagca
    33841 gtttgcgctg gtggacacga tttgccgtca ctttcccggc gcggagtttc taaccagcgg
    33901 tgatttgggc ttaacgccgg ggctgaatca accgcgtatt acccaacggg tggagcaggt
    33961 gctggctgat gcatttcacg cacaggctgc ggcgctggtg cagggcgcgg ggactggcgc
    34021 gattcgcgcc gggctggcag ctttgctcaa accggggcag cgtcttctgg tgcatgacgc
    34081 gcctgtttac ccgacgacac gggttattat tgagcagatg gggctgacgc ttattactgt
    34141 tgatttcaat gacctgtcgg cactgaagca ggtcgtcgac gagcaacaac cggatgcggc
    34201 gctggtgcag catacgcgcc agcagccgca ggacagctac gtgctggcag atgtgctggc
    34261 aacgttgcgc gcggcaggtg ttccagcgtt aaccgatgac aactatgcgg tgatgaaggt
    34321 ggcgcgaatc ggctgtgaat gcggcgcgaa tgtctcgaca ttttcctgct tcaagctatt
    34381 tgggccagag ggtgttggtg cagtggtcgg cgatgctgat gttatcaacc gtattcgcgc
    34441 cacgctttac tccggcggta gccagatcca gggcgcacag gcgctggaag tattgcgttg
    34501 tctggtgttt gcgccagtga tgcacgcggt gcaggcaggg gtatctgaac ggttgctggc
    34561 tttgcttaac ggtggtgagg tgccggaagt gaaaagcgcg gtgattgcta atgcgcagtc
    34621 gaaggtgttg attgtcgagt ttcatcagcc gattgccgcc agagtgctgg aagaggcgca
    34681 aaagcgcggt gccttgcctt acccggtggg tgcagagtcg aaatatgaaa tcccgccgct
    34741 cttttatcgc ctttccggaa cgtttcgcca ggcgaatcca caatcagaac attgtgcgat
    34801 tcgcattaac ccgaatcgca gcggtgaaga gacggtgctg cggattttgc gtgagagtat
    34861 tgccagtatt taatgctgga tagcaccgtc aagctaaatt ccgtactgaa cggtcccctc
    34921 gcccctttgg ggagagggtt agggtgaggg gaacgtacgg gatgtaaaat tcaacgcgcc
    34981 agcatcgccg gatgcgatgc tggcgcatct tatccggcct acggttaatg tcttttatta
    35041 tcaatagtta aggtgatgcg attggcgcgg cagaagagtt cggagacgtg caccggtttg
    35101 tcattctgat caaaggcgat tttgctgatg cgaaacaacg gttcgcccag ttcgcatttc
    35161 agccattttg cctgcgggcg ggtggcggca aagatatcga tggtcttttt gtcgctgacc
    35221 actcgcgtgt caaaacgctc ctgaaataac tgataggtgg agcttccttc gacgtaaatc
    35281 tcatcaaagt caggataacg cgacagcggg atccatgaac tatcaataaa cagcggctct
    35341 ttatcgagat acatcacccg gcagagatgg aacacttcgc tgtttccggg gatgttcagc
    35401 tttttgcaaa acggcgcggc gctgacccgt tcctgttcga tcactttctc tttcgtcgcc
    35461 ttgccttgtg agacgccaaa atcggtaaaa ccactgacag taagcagggc gttttcaact
    35521 ttctggcttt gtacaaaggt gccttttccc tgccagcgga tcagtacgcc gtccgccact
    35581 aagtcgctga tggctttgcg aatggtaatg cggctgacgt tatattgtgt acaaagctcg
    35641 ttttcggtag ggatctgttg cccggcctgg taaaccccct gcgcgatatc atccagcagt
    35701 cgctggcgga cggtagcgta gaggagttga tgagagtagc ggtccgtagc tgacataatc
    35761 gaccctgaaa taaagaatga attactcgga ttatagggtc gttttccggc aagtaacagc
    35821 acgttggcgt gaagatgctc acgccaacgt tatacctacc aggcaccgtg gtactgaatg
    35881 gttttcgccg cgcacgccgt tccctgcgcc atcgcctgcg gtaatgtcat ccccgcagac
    35941 cagccgcaaa ggaatccggc aatgaacgaa tctccggcac ccatggtgtc gataaccgtc
    36001 accggttcag gagcctgacg ccagaactgc gcgccatccc aggcaatgct gccgttttca
    36061 cccagcgtga caatcactgt tcctgcgcca cgggcaacaa tcgctttcat cttcagacgc
    36121 agcgtttcgt cttcttgcgg tgcggaggca aaggcaaaat cgagatgcgg caccagtgtc
    36181 tgccagagcg ggctgtccca cttgtcggag aagtcgaaag cggtaagttt gcccgcagcg
    36241 tgcagctgtg ggaatgcgtc ttccgcatgt ccccagattg ccgcgtgcac aatgtcatac
    36301 tgcgccagcc aggcgtaatc ctcttcactc agggcaaagt cggccatcac gccttcggtg
    36361 tagtcgccaa aaacgcgatc attgtcgtgc agttccacct gagtttgtgc ggtaacgccg
    36421 tgtttcgtat ggacatggct gatatcgacg cccatgcggg cgagatcctg cttcagcttt
    36481 gtgccgtagt catcgtcacc cacccaggta atgcatcccg gctgtatgcc gtagcgagtg
    36541 cagtacaccg ccacattgac cgcattaccg ccagaaaacg ctttattcag ttgcgggtag
    36601 atatcgacgc agttatcgcc gattgtcgcc agggttttca tctctcatcc tccggcagca
    36661 gcgcgcgaaa gcgttccagc gcctggcggg catagagtct gggctcgttc atatacatcg
    36721 tcaccagctc caccgtacag taaccctcat agccccgctc aataatatcg cgcatcagtt
    36781 cccgcagcgg cattttgcct tctccaggaa tgtaatgcgt gtcgctggcc ccgtcgctgt
    36841 cgacaatatg cagatgacgt aatttatcgc ccagtttgtc gaaataactc atcaccggtt
    36901 ccgcctggac atacggcgcg caaatgtcga ccatgctgaa caggcgcggc gaaggcacca
    36961 gcgccagcgc atgaagcaca tcattagcat tacacacgat gttcgattca tacggcgtta
    37021 acggttcgag gattagatcc atgccgatgt tttccgcgta ctcatacagt tcgcttaggt
    37081 tctctgccag ccgtccccag ataacattag gtggcgtgag atagcccgcg tgggccgcgg
    37141 aaatcagcgt ataacccgcg ttcatctctt ttgccatatc catcgccagc ttgatcatgt
    37201 cgaggctttc gcgacgcata tgttcatcgc ccagcatcat gttatacgga tagccattgg
    37261 tttctggcgt atagccgata atcggcatct gatacgtctg cgccagcgcc ttgatttgtt
    37321 tgatgccgcc cgcttttaag tccggcgcga acgcgtgcgg gcgaccgccc caaatttcga
    37381 tgccgtcgta acccagctcg cttgcatcac gaaatgcgtg ttcaataggc agccgctggt
    37441 ggccgcaggt aaacatacct gtttttatcg ggtaactcct tgcgtaaatc cggcccgttg
    37501 ccgggccggg cgagggatta atattccacc agaccaccgt aatagcggcg ttcatccggg
    37561 ttgtgatctt tgtaaataga caggtagtag cagagccact ccattggcac gaacatcagg
    37621 aacggtgcca gccacgggtg cagcccttgc gaaatttcgg cgtaatcgat gacgatcacg
    37681 ttgtcagtac gctgtttaac aaagttaatg gcgcgttcgg tggtgtggcg actttcatca
    37741 ttgccgagca ggaacaggaa cggaacgccc ggttcgacaa tctccagcgg gccatggcgg
    37801 aactctccgc tctcaatcac gcagccgtgc gtccaggtaa attccatcag cgttacaatg
    37861 ccttctttgt aacccagcgg acgcagcgga cccgcagcaa cggtataaat catcggccac
    37921 tggctggcca gttcaccaag ctggcggcct ttttcttccc aggtgcgtac cagatgaccg
    37981 agcgcattcg gcaactgctt gagatcgttt ttgatcttgc cgatttccgc gttcggcgcg
    38041 aggcgggtga tcatctccag caccacgctg tagcagagca gcaggtgaat ttcccagata
    38101 cagtcggcct gataatcaat gctaaattcc gccgccgagg taatcgggct gtccgcgcgt
    38161 ttggtgaacg ccgcagtgag tgcgccgcag gcccggccca gctccagcgc tttgattacc
    38221 tcttccgttt taccgtagtc agaaacgcca atcaccgcgc aacgatcgtc gaggcgatac
    38281 ggggtgttat cgcagaactc ccagccggaa atggcgtaga cctgaagatc ggaaaagcga
    38341 tccgccagat gtttcgccgc ttgcgccgcg ttgagtggcg agccgcaggc aacgaaataa
    38401 atacgatcaa tgtcgcgctt caccatctct tccacgatgg cgtgcaccag cggaacgtca
    38461 tggctgagaa ctttttccac ttcctgaacc atattttcgg tgaccagaaa gtccacggtg
    38521 cttttatcaa tatccaacat tttattttct ccagataaca ttacgacaaa gcgttgagct
    38581 ggcggctacg cttcgcccag aaggcgtaag caggcagacc agtagcaata acgatgacgg
    38641 cgcagataag gccgggaatc ggtgcccaga caaaggttga ggcgaccaga atgaggcttg
    38701 acgcaatggc gagggtggtc atcagcccga aagcaggagt acgccacagc ggtttgtaat
    38761 cgtcgcgttt acgacaccag atgatggagc cgaaggtgag ggtatttttg aaacacatca
    38821 ccagggtgaa ataacccagc aggctggtga gatcggaaac gaagatgaag aagagcccca
    38881 gcgccccttg caggatgatg gagacatccg gcgtgttgta tttcggatgc acatggccga
    38941 agcatttaaa gaacaggttg tctttcgcca tcgcgtattc cagccgcggc tggtacatca
    39001 cgcagctgga aagcgaacca agaatgacga tcatcgccgt gatggcaaca aagatcccag
    39061 cggtgctgcc gagtgcgggg atccaggtca gggcgtcgga aatcggcgtt tcagaattgg
    39121 cgagtttgtc gaagggcatc aggtcggaaa tcaccagcgc cagcagggtg tagagcacca
    39181 gaaccagcag acaggaacca atcagcgctc gtggcatggt ttttccgggg tttttaattt
    39241 cgccggtcat ataacagata gaggccatgc cggtatacga ccaactggtg gcagagatcc
    39301 ccgccagcag cgccataaag ctgcccgttg cgccaatcgc agtggtggta ggggcggcaa
    39361 aattctccgc tttaaaccag aagatcccaa ggccaatgac gatagtgaac gggataattt
    39421 tggcgatggt aattagcgtc tgaaacgctg cgccgccttc aacggagcgc aggtgtagca
    39481 acataaaggc gataattaat ccggcggcga taaatttacc gggcaacgga tcgataggcg
    39541 ttaaaaagcc aagattgctg acaatcgcca gcgccataat cgacaatgac ggcgcatcgt
    39601 tggcccagaa gctggcccag ccggagagga aagccagcgg tcggcttccg gcatttttca
    39661 gataaacata atctgcgcca ttttccggat aagcggtgga tagttccgca tagacgcaca
    39721 tttgcgggat cacaattaac ccgccaatga caaacgtgag caccgtaagc cacggcgtgc
    39781 ccgctgcttt tgccacttca cccacagata caaaaatacc ggagccgacg gttgtcccga
    39841 cggcgattgc aagaacggcc caaaatccga gcttgcgttg gagttcctgg cttcccatag
    39901 cgataccttt ttcttgtcca acacgttgta ggggagagtt tgcagtcatt taaaaggtaa
    39961 catgacaatg catgatgaat ataataacat atgatgttat gtaatgcgga ggaagatcac
    40021 aaaatttcgc acaggatcgc gctgtggcta atgaatgtag ttatccaatt gaaattaaag
    40081 tgaaaatatt tttacgggtg gggagcaaga aggatataga aacaaatacg ccctcggaaa
    40141 atccagaggg cgtcgggcaa ttaaaccggt gttagccgat ttctgtcaga gacttactgt
    40201 gcagtaggac cgcaaccgcc aatgatttta gaaatagaaa ttgccgggtg cagcaggtag
    40261 tcgtaagagc agttcttatc acggttttca atatgacca
//
LOCUS       DAJVOY010000013        39911 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVOY010000013 DAJVOY010000000
VERSION     DAJVOY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.013"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(447..2138)
                     /gene="fruA"
                     /locus_tag="OQL90_000625"
     CDS             complement(447..2138)
                     /gene="fruA"
                     /locus_tag="OQL90_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1367506.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2155..3093)
                     /gene="fruK"
                     /locus_tag="OQL90_000626"
     CDS             complement(2155..3093)
                     /gene="fruK"
                     /locus_tag="OQL90_000626"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1367507.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3093..4223)
                     /gene="fruB"
                     /locus_tag="OQL90_000627"
     CDS             complement(3093..4223)
                     /gene="fruB"
                     /locus_tag="OQL90_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1367508.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4591..5772
                     /gene="setB"
                     /locus_tag="OQL90_000628"
     CDS             4591..5772
                     /gene="setB"
                     /locus_tag="OQL90_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1367509.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5769..6023)
                     /locus_tag="OQL90_000629"
     CDS             complement(5769..6023)
                     /locus_tag="OQL90_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1367510.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6178..6750
                     /gene="yeiP"
                     /locus_tag="OQL90_000630"
     CDS             6178..6750
                     /gene="yeiP"
                     /locus_tag="OQL90_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1367511.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6973..8439
                     /locus_tag="OQL90_000631"
     CDS             6973..8439
                     /locus_tag="OQL90_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1367512.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8557..9543
                     /gene="yeiR"
                     /locus_tag="OQL90_000632"
     CDS             8557..9543
                     /gene="yeiR"
                     /locus_tag="OQL90_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1367513.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9582..10295
                     /gene="lpxT"
                     /locus_tag="OQL90_000633"
     CDS             9582..10295
                     /gene="lpxT"
                     /locus_tag="OQL90_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1367514.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10707..11273
                     /gene="mepS"
                     /locus_tag="OQL90_000634"
     CDS             10707..11273
                     /gene="mepS"
                     /locus_tag="OQL90_000634"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1367515.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11454..13010
                     /locus_tag="OQL90_000635"
     CDS             11454..13010
                     /locus_tag="OQL90_000635"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1367516.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13092..14906
                     /locus_tag="OQL90_000636"
     CDS             13092..14906
                     /locus_tag="OQL90_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1367517.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14907..16001
                     /locus_tag="OQL90_000637"
     CDS             14907..16001
                     /locus_tag="OQL90_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1367518.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16001..17026
                     /locus_tag="OQL90_000638"
     CDS             16001..17026
                     /locus_tag="OQL90_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1367519.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17028..18617
                     /gene="yejF"
                     /locus_tag="OQL90_000639"
     CDS             17028..18617
                     /gene="yejF"
                     /locus_tag="OQL90_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1367520.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18621..18965)
                     /locus_tag="OQL90_000640"
     CDS             complement(18621..18965)
                     /locus_tag="OQL90_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367521.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19298..20481)
                     /gene="bcr"
                     /locus_tag="OQL90_000641"
                     /pseudo
     CDS             complement(19298..20481)
                     /gene="bcr"
                     /locus_tag="OQL90_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20509..21204)
                     /gene="rsuA"
                     /locus_tag="OQL90_000642"
     CDS             complement(20509..21204)
                     /gene="rsuA"
                     /locus_tag="OQL90_000642"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1367522.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21353..23113
                     /locus_tag="OQL90_000643"
     CDS             21353..23113
                     /locus_tag="OQL90_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1367523.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23238..23522
                     /gene="rplY"
                     /locus_tag="OQL90_000644"
     CDS             23238..23522
                     /gene="rplY"
                     /locus_tag="OQL90_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1367524.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23661..24668)
                     /gene="yejK"
                     /locus_tag="OQL90_000645"
     CDS             complement(23661..24668)
                     /gene="yejK"
                     /locus_tag="OQL90_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1367525.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24850..25077
                     /locus_tag="OQL90_000646"
     CDS             24850..25077
                     /locus_tag="OQL90_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1367526.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25097..26857
                     /gene="pbgA"
                     /locus_tag="OQL90_000647"
     CDS             25097..26857
                     /gene="pbgA"
                     /locus_tag="OQL90_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1367527.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26932..27008
                     /locus_tag="OQL90_000648"
     tRNA            26932..27008
                     /locus_tag="OQL90_000648"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26966..26968,aa:Pro,seq:ggg)
     gene            27227..28468
                     /locus_tag="OQL90_000649"
     CDS             27227..28468
                     /locus_tag="OQL90_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1367528.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28964..29170
                     /locus_tag="OQL90_000650"
     CDS             28964..29170
                     /locus_tag="OQL90_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCS1367529.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29185..29850
                     /locus_tag="OQL90_000651"
     CDS             29185..29850
                     /locus_tag="OQL90_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367530.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29876..30076
                     /locus_tag="OQL90_000652"
     CDS             29876..30076
                     /locus_tag="OQL90_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1367531.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            30066..30287
                     /locus_tag="OQL90_000653"
     CDS             30066..30287
                     /locus_tag="OQL90_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367532.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30280..30513
                     /locus_tag="OQL90_000654"
     CDS             30280..30513
                     /locus_tag="OQL90_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367533.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30506..30739
                     /locus_tag="OQL90_000655"
     CDS             30506..30739
                     /locus_tag="OQL90_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367534.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30745..31044
                     /locus_tag="OQL90_000656"
     CDS             30745..31044
                     /locus_tag="OQL90_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1367535.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            31041..32441
                     /locus_tag="OQL90_000657"
     CDS             31041..32441
                     /locus_tag="OQL90_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1367536.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVISIDGDDVAPL"
     gene            32641..32892
                     /locus_tag="OQL90_000658"
     CDS             32641..32892
                     /locus_tag="OQL90_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367537.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            32889..33299
                     /locus_tag="OQL90_000659"
     CDS             32889..33299
                     /locus_tag="OQL90_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS1367538.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            33310..33582
                     /locus_tag="OQL90_000660"
     CDS             33310..33582
                     /locus_tag="OQL90_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1367539.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            33821..34027
                     /locus_tag="OQL90_000661"
     CDS             33821..34027
                     /locus_tag="OQL90_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367540.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            34027..35082
                     /locus_tag="OQL90_000662"
     CDS             34027..35082
                     /locus_tag="OQL90_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1367541.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            35094..35429
                     /locus_tag="OQL90_000663"
     CDS             35094..35429
                     /locus_tag="OQL90_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1367542.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            35442..35855
                     /locus_tag="OQL90_000664"
     CDS             35442..35855
                     /locus_tag="OQL90_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1367543.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            36017..36451
                     /locus_tag="OQL90_000665"
     CDS             36017..36451
                     /locus_tag="OQL90_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367544.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            36732..37013
                     /locus_tag="OQL90_000666"
     CDS             36732..37013
                     /locus_tag="OQL90_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367545.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(37692..>39911)
                     /gene="yejO"
                     /locus_tag="OQL90_000667"
     CDS             complement(37692..>39911)
                     /gene="yejO"
                     /locus_tag="OQL90_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCS1367546.1"
                     /translation="ANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQK
                     AGGAIKASTRVMEAFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGG
                     LLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLI
                     VMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMV
                     INNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVD
                     TSKADVSLENSVWAIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAEN
                     FITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTL
                     VTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAA
                     QPLVFDAELDTVRERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGI
                     DSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVK
                     VDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQ
                     DYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKV
                     NDDGKFNNDVAGTPGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT        10041 a        10151 c         9994 g         9725 t
ORIGIN      
        1 tacgacataa tccttttcgc gcatagcaca tcctctggta aaaaattacc aaccctgtca
       61 atccacaaga atatagatac tgcaaagacg gtttaaacat gtgttcatat ttaaacatgt
      121 gctcatagca tgcatgtttt acgtaaagta aattaccgcc agagaggcaa aatgggcaga
      181 tttgagcagg ctcacaaaat gcgattaaat gcctgaatta caaaagcgag gaatttgtga
      241 cgggatgcac agaacgggaa caataaaaaa ggcagaaaat aatctgccct tgaggaaata
      301 accttagtgt ttgatgaacg tttcaagttt ctactgaaag ataaaaccat ttaaattcat
      361 aaaattacat tcacaaaccg gtcattctct ttgcgctcat caaatgttac agggcaggaa
      421 atttctgccc tgtaacacac cttttattac gctgctttcg ctactgcgtc cacttccgga
      481 cgtttcagga aggcataggc caaacccgcc accagcgtac cggcaataat tgctaccagg
      541 taacccagta ccggcgtaat agcgccaggg atcagcagaa caaacagacc accgtgcggt
      601 gccatcagtt tcgcaccaat cgccattgag attgcgccag tcagcgcccc acccacgata
      661 cagcacggca gcacacgcat cggatcacga gcagcaaacg gaattgcacc ttccgaaatg
      721 aagcacagtc ccaataccag agcggctttg ccaccttcct gctgcgcttt gtcgaatttg
      781 cgacgcgcca ccattgttgc cagacccatt gccagcggtg gcaccatacc tgccgccata
      841 atcgccgcca tcgggccata ggtttgagta ctcagcagac ccacaccgaa tgcgtacgct
      901 gctttgttta ccggaccgcc catgtcagta cacatcatgc caccgaggat cgcccccagc
      961 agaaccgcat tcgcagtccc catggtctgc agccagtgag tcagcccttc gagaatgcca
     1021 gcaactggtt taccgatcag gtagatcatc gccagaccga ccaccagact ggaaattagc
     1081 ggaatgatca ggatcggttt cagcgcctcc atactctgtg gcagtttcag ttgcgtactg
     1141 attaacttcg caatgtaacc agccaggaag ccagcaatta taccgccaat aaagccagaa
     1201 ccggtgctga ccgccagcat accgccaatc agacccggcg tgaggcccgg acgatcggca
     1261 atggagaagg caatataacc tgccagtacc ggcaccatca gcgcaaaggc tgaaccaccg
     1321 ccaatctgca tcagcgccgc tgccaacgta cccggctctt taaacgcttc gataccaaaa
     1381 gcaaaagaaa gcgcgataca cagaccacct gcgacgacca tcggcagcat ataagagacg
     1441 cctgtcagca agtgacggta agcgcctgca ctctctttct taccttcagt ggtcgccgtt
     1501 tgagctttgc ccgccggttc atacggcgtt gcttcagcaa ccgctttatc cagttcctgc
     1561 gcggttttct tcagcgccag accggtagag gtacgataca tcggtttacc agcaaatttc
     1621 gccagatcca cttcgatatc tgccgccaca atcaccagat ccgctgctgc gacttcttca
     1681 ggggtgattg cattgcccgc gccaacagaa ccacgggttt caactttcac ccaccagcca
     1741 cgttttttcg cttcggtttc aatggcttca gccgccataa aggtgtgtgc tacgccagtc
     1801 gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg tgctgtcgca
     1861 gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt cactcaggaa cagctcaggg
     1921 tgcgcaactg cccgggaaat atcacccagc cagacatttt taccgttcag cgcgctgtca
     1981 ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt cgttcggatt gtcgatgatt
     2041 tccagttttg cttttcgcgc caccgcgccc agcagggtct tcgccatata ggcgcgtgcc
     2101 tgaccgagat tagcgtcaat aatcagcagc gttttcatta tgcctctcct gctgtcagtt
     2161 aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac tgcggacgat cggtaatacc
     2221 cacattgctt tgacttaccg ccagggctgc aacagctgtc gccagacgca gtgtgtgttc
     2281 actggattca cgcatcagca agccataaat caggccacca accatagaat cccctgcgcc
     2341 aacggtgctt acgacatcga ctgacggtgg tttggcgatc cattcgccgg aggcattaac
     2401 ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc gcaatacctt gttcacgtag
     2461 cgcatgtgca gcttcaatca catctttcat ttcaggcagt ttacggcctg cccaaatttc
     2521 caactcgcgg cggttaggtt tcaccagcca cggtgccgct ttcaaacctg ctactaacgc
     2581 ttcacggcta ctatcaaaga taatgcaagg acactgacta cgcaggcgag tcatccagtc
     2641 ggtgaacgct tccgggctga cgcctgacgg taagcttccg ctgacacaga ccatatcgaa
     2701 ctgaccgagc cagctcagag aatcagtcac aaagcgttcc cagtcggcgg gggtgacttc
     2761 aaaacccgag aagttgaagt cggtcacttc gccgtctttt tccgtcagct taacgttaat
     2821 tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg cccagctcgc tgaacagttg
     2881 ctgaaaacca tcctgattgt ctttacccag gaagccgcca acggtgacat caattcccag
     2941 gtcttttaat actttggcca cgttgatgcc tttacccgcc gcatgcagac cggtggtttt
     3001 caccaggttc acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt cataagccgg
     3061 attaagggtg atcgtagcaa cacgtctgct cattatgcgc cctccccaag accagcagcg
     3121 atagcgtcgc caatggcttt cagcgcctgt tcagcatctg caccctgggc ggtaaagcgt
     3181 aggcgatgac ctttcttaac gccaagtgcc acaactttca tcagactacg tccgtttgcc
     3241 ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac tgttaaattg tttaatggta
     3301 ttgaccagca tggtacctgg acgagcatgc aggccgtgtt cattgcgcac cacaaactcc
     3361 gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag caacgttgcc
     3421 gcatctgctt tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc gagacgctta
     3481 agaaccgcga tgggctgatc gtcattcatc gccacactca ccagcatggc tgccgtttcg
     3541 ccgtccacag caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg cagattgcct
     3601 tcggcgctat cgctcagcca gataccctgt ccgagattca gcggttgttc attgatggct
     3661 ttggtgacga aagtggcgtc aactgccccc gcctctttca gacgcgcagc gttcagcgcc
     3721 tgaagagtca gcagatcgct ggcgacgata tccagcgtca gcatttcgtt gtcgagcttc
     3781 agctgctcac tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc tgttgttgct
     3841 gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg gcgtagcagg
     3901 cccagatgtt catccgagct ggcagcaata ccgattgcca cgtacgctac ctgaccgtca
     3961 ccccaggtga cgccttccgg gaactgaaat acctgaacgc cggttttcag cacctgatcg
     4021 cgggtgtcgg tagtgccgtg tggaatagca ataccattgc cgaggaacgt tgaggtttgc
     4081 tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta cattaccggc ctgcaccagc
     4141 gccgcagcga cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc cggatggatg
     4201 tcctgtacgg ataactggaa catagttctc ctctcttgct gaattgaaac gattcagcct
     4261 ctatgagaaa aaaagcgcca acctggctta gggttaaaga caagatcgcg ctgaaacgtt
     4321 tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt tatgagcaaa
     4381 agtttgatgt gctgcacatt tttttgtcat ttttcctaat tgctgatggg aaaatccgtt
     4441 atcagcaatt tcatttcagc agcgtcagca gccaatcagc aacagtgaaa tggcattttg
     4501 attattcgtg gcgcttttga tttcgctgtc tgtttatttt ctgacaagca gcgtaaactc
     4561 cgcgtcttcc tcttccagtg atagacctgc atgcataact cccccgcagt ctccagcgcg
     4621 aaatcgtttg acctgacctc gacggcgttt ttaatcgttg cctttctcac cggtattgcg
     4681 ggtgctctgc aaaccccgac actcagtatt ttccttaccg atgaagtaca tgcccgtccg
     4741 gcgatggtgg gattcttctt taccggcagc gctgtcattg ggattctggt aagtcagttt
     4801 ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt ttgctgcctg
     4861 ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca actactttgt tttgctattc
     4921 gttggcgtct ttcttagcag ctttggctcg accgctaacc cgcaaatgtt tgcccttgcc
     4981 cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt tttacgcgct
     5041 caggtttcac tggcatgggt cattggccca ccgctggctt atgcgttagc gatgggtttc
     5101 agctttacgg taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg cgtgatggtg
     5161 tggctgtttt taccgtcgat gcaaaaagag cttccgctgg cgaccggcac ggttgaagcg
     5221 ccgcgccgta accgtcgcga tacgctactg ctgtttgtca tttgtacatt gatgtggggc
     5281 tcgaacagcc tgtacatcat caacatgccg ctatttatta tcaacgaact gcatcttccc
     5341 gagaaactgg ccggtgtgat gatggggacc gccgccgggc tggaaatccc gacgatgttg
     5401 attgccggat atttcgccaa acgtctgggt aagcgtttct taatgcgcgt tgctgccgtg
     5461 ggtggcgtct gtttttacgc aggaatgctg atggcgcatt cacctgccat tctgttgggc
     5521 ttgcagctgc taaatgctat ttttattggc attctgggcg gcatcgggat gctctatttt
     5581 caggatttga tgcccggtca ggcaggttca gccaccacgc tctataccaa cacgtcgcgc
     5641 gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg gaattatcac
     5701 gctgtgttct ggtttgcgat ggtgatgatt atcgccactc tgttttgctt actgcggact
     5761 aaagatgttt aagacgcggt cagcatttcc agatcgataa gccaggtcat ggcctgctga
     5821 cgcgagttac cgcacatttc agcgctggct tgtaagcctg cacagacttt cggtcgcagt
     5881 ggcgaggtga aaattttaca gcgttgctgt tcatcaagct gaatgcaggg cgtattggcg
     5941 ggcttgccat ctggcatacc gggaataggg ctgaaaattg aaggggcagt gcaacacgcc
     6001 ccgcaacccg gacggcattc catacgttgt tctcttaagg ttaaccaccg gattgatgag
     6061 cgcgcacagt accacctttt ttgcaccagc aaaagtgcga ataccacttg cccgaaaggc
     6121 ccgtcgcgag tactttgtcg cgatattttt gacattttcg actacaggaa tttttcgatg
     6181 ccaagagcga acgaaattaa aaaaggtatg gtactgaatt acaacggcaa actgctgctg
     6241 gttaaggata ttgatattca gtcgcccact gcccgcggtg ccgctacgct gtacaaaatg
     6301 cgtttttctg atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg tgatgatatc
     6361 gttgacaccg tgacgctgac ccgccgttac gttgacttct cctatgtcga tggcaacgaa
     6421 tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga tcagattgaa
     6481 gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct gacctgggac
     6541 ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt tgaaaccgca
     6601 ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt gagcactggt
     6661 ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat ccatatcgaa
     6721 gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa gggatagctc
     6781 aggctgcccc ttttttaatt tattatacca atcttctatt ttgcgcttcc tgaacacctt
     6841 acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat taacaatcca
     6901 gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta tcgttactaa
     6961 gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt acacgctcca
     7021 cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg cgcctttcac
     7081 cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg cgactggggg
     7141 atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg cgcacagaac
     7201 catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat tgtcggtgcc
     7261 gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga gaaattttgc
     7321 gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg tattgacccg
     7381 gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca aacccctgaa
     7441 gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg tgagcgcggc
     7501 cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca tgtggtgaaa
     7561 aacgcggtgc tgggaatggc agaaaaacgt tcgccagaac tcgccgggtg gataaaagaa
     7621 cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac cgacgaatca
     7681 ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag ctgcgaaccg
     7741 tttatccagt gggtggtgga agataacttc gtcgctgggc gtcctgcctg ggaagtcgca
     7801 ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg tatgcttaat
     7861 ggcagccact cttttctcgc ttatctgggt tacctttcag gattcgccca tatcagtgat
     7921 tgcatgcagg atcgcgcatt tcgccatgcc gccagaacat taatgctgaa tgagcaagcg
     7981 ccgacactac aaattaaaga tgtcgattta acacaatatg cggacaagtt aattgctcgt
     8041 tttgctaatc cggcgctgaa acataagacc tggcaaatcg caatgaatgg tagccagaaa
     8101 ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgcgaaac ggactggtcg
     8161 ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga tgatgccgga
     8221 aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt tgcgggcagc
     8281 agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg tgatgatctg
     8341 ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat cgtacaattc
     8401 ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg gttaaagcgg
     8461 atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag ccaggattaa
     8521 cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa cctcatcacc
     8581 ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca taaagatccc
     8641 aacgaaaaat ggacggtact ggttaatgaa tttggggaag tcggaattga tggtgctttg
     8701 ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg ctgcgttaat
     8761 ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc agaccgcttg
     8821 ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt aaccgcacca
     8881 gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc acgcttgctg
     8941 ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc cgcagacatc
     9001 attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct acagcgttgg
     9061 tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa agttgacggt
     9121 catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc cgcgcattct
     9181 catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca tcaacgctgg
     9241 cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt cgacgctgat
     9301 acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt ggaacgcgtc
     9361 aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg cgatgacctg
     9421 cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat ttccagcagc
     9481 gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc gactaccgcg
     9541 taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa aatttgccgc
     9601 aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct ttcctggtat attcccgtta
     9661 atcatggatt atggttgccg attgatgcgg atatttttta tttctttaat cagaaactgg
     9721 tcgaaagtaa ggccttttta tggctggttg cattgaccaa caatcgcgct ttcgacggtt
     9781 gttctctgct ggcgatggga atgttgatgc tgagtttctg gctgaaagaa aacgccccag
     9841 gcagacgacg tatcgtgatt attggtctgg taatgctatt aactgcagtg gtattaaacc
     9901 agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgacg tttactaata
     9961 ttaaccgcgt cagcgaactg ctctctgtcc ccacgaaaga tgcctcacga gatagctttc
    10021 ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt tatttcggta
    10081 aagttgcagg tcttatcgcc cttattattt ttgtggtttt tgcgtttccc agagtaatga
    10141 ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata ttgatcggtt
    10201 tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt gacaaatcac
    10261 taccgggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac atcatcaggt
    10321 ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct atcgaacgca
    10381 aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg tactttaatt
    10441 gaccaatccc gcttattaac tttctgtatc actttttctt ataaaaaatc atgtaaaacc
    10501 gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg cggtagatta
    10561 tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg aattgtttca
    10621 acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat atttgtcgtt
    10681 aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg agatatatct
    10741 tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt gcaaataaca
    10801 ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca tcactgcaag
    10861 cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg cgaattatgg
    10921 atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact aaaaaaggta
    10981 tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta gaacttccgc
    11041 gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat ttgcgtacgg
    11101 gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt tatatcggca
    11161 acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc atgaatgaac
    11221 cgtactggaa gaagcgttac aacgaagcac gccgagttct cagccgcagc taataaaccg
    11281 tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct taggtagtgc
    11341 ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca ggctatagtc
    11401 cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga atcatgttca
    11461 tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca ggcgtgatga
    11521 ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac gaagtcaaat
    11581 acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc gacctgaaat
    11641 ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct aaccccgaac
    11701 tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg aaagataaaa
    11761 aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa ttgattccgg
    11821 cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg atggtgccga
    11881 acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc ggtgtgtttg
    11941 ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag gaagattacg
    12001 atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca cgtttgatga
    12061 acgttaatga attaaccgac atgcctgtcc gtgaaactaa aattgcgggc attcctctga
    12121 ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca tttttactgg
    12181 gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc gtacgtatgc
    12241 gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg gcgtatcaac
    12301 cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta cgctggcggc
    12361 atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa tcgcaaaaga
    12421 tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc gcagaattag
    12481 aaaaagtgct gccagtaggc gtcaaatttg gtattaacat tgcgccggac catctgcaca
    12541 gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca caccatttcc
    12601 agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc acacaactct
    12661 tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc accgggcaca
    12721 gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac cgtggattta
    12781 tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg ctgacgctgg
    12841 cgaaacgcct caatatgctg acggttgctg aagggggcga aacgccggaa caggcgcgat
    12901 ggctaagcga acccggcgtt aatttcatgc aaggctactg gattagtcgc ccgttaccgc
    12961 tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa ggtgtgctta
    13021 cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga gtaaaaggaa
    13081 atccgttgca gatgattgtg cgcatactgc tgctgtttat cgccctgttc accttcggtg
    13141 cgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa ccccggtacg
    13201 catttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt gggcagataa
    13261 cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc ggcaacccgg
    13321 gcgcacgcac cgagcagttg tacgacacgc tatttacgac ttccgatgac gaaccaggca
    13381 gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc tgggtggagg
    13441 tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc cattactgcc cgcgatgtag
    13501 agtttacttt tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg gtctacaaag
    13561 gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat tgagttagct aaacccggca
    13621 aagaagatat gctgagtctg ttttcgctgc cggtatttcc agaaaagtac tggaaggatc
    13681 acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac cgcgttacgt
    13741 cctggaaaat ggggcaaaat attgtctatt cccgtgtgaa agattactgg gcagcaaact
    13801 taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat tacctcgatg
    13861 ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg gaaaacgacg
    13921 ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac atcatcaaag
    13981 atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat atccaacgtc
    14041 cggtattcag cgatcgccgg gtccgggaag caatcactct cgcctttgac tttgaatgga
    14101 tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt cagaataccg
    14161 aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca ccaatgaaaa
    14221 aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct aaaggcgatg
    14281 gctacgatcg tgacaacctg ttaaaagccg acacacttct caacgaagcg ggctgggtgc
    14341 tgaagggtca gcaacgcgtt aatgccacaa cgggtcagca actcagcttt gaattattgc
    14401 ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg caacggctgg
    14461 gtatcaacat ggacattcgc aaggtggata actcgcaaat caccaaccgc atgcgcagtc
    14521 gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt tccgatttac
    14581 agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc gtgcaaagcc
    14641 cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa gaaaaattat
    14701 tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg ctgccaatgt
    14761 ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccacccc gctgtacgcc
    14821 ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg gccaaactgc
    14881 cgtcagccag gcaacaggga gagtagatgg gcgcttacct gattcgccgt ctgttgctgg
    14941 tgatcccaac attatgggcg attatcacca tcaacttttt catcgtgcaa attgcgcctg
    15001 gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga gtattacccg
    15061 gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc aatatcagcg
    15121 acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag atcactcatc
    15181 gctacggttt tgataagccg atccacgaac gttacttcaa aatgctctgg gactacatcc
    15241 gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg attaaagaca
    15301 gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat ctggtgtcga
    15361 ttccgttagg cattcgcaaa gctgttcata atgggagccg ctttgacgtc tggagtagcg
    15421 catttatcat tatcggctac gccattccgg cctttttgtt tgccatcctg ctgattgtct
    15481 tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt tccgctaact
    15541 ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc acgctgccgg
    15601 tgctggcgac ggtgattggt ggctttgcgg cgctgaccat actgacaaaa aactcattcc
    15661 ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta agtgaaaaaa
    15721 atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc ggttttccgg
    15781 cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg ttttcactca
    15841 atggtctggg cttactgggc tacgaagcga ccgtctcgcg cgattatcct gtaatgtttg
    15901 gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt gatatcagct
    15961 atacgctggt tgatccgcgt attgattttg agggacgtta atgtcgcgac tcagccccgt
    16021 caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt cgttatggat
    16081 tttcctcgtc ttgtttggtt tgagtttgtg ttctgagctt atcgccaacg ataaaccatt
    16141 gctggtacgt tatgacggca gttggtattt cccgttgttg aaaaactaca gcgaaagcga
    16201 ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga aacaacggct
    16261 ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca gtatcaactt
    16321 tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg gaacggatgc
    16381 caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg ttctgtttgg
    16441 cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc tacaaggcta
    16501 ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt cggggatgcc
    16561 gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct ggtggctgct
    16621 ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg cggagttttt
    16681 acgtactcgt aatttcgact acattcgtgc ggcgcaggcg cttggcgtca gcgatcgcag
    16741 tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct ttttaccgtt
    16801 tattttatgt agttcgatca ccaccctgac ctcactcgat ttcctcggct tcggtctgcc
    16861 gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc ttcaggctcc
    16921 gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc tgatctttat
    16981 tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg acgcaaactc
    17041 tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta cgtacagtag
    17101 tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg ggtgagtcag
    17161 gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc ccgccggttg
    17221 aatatctctc cggcgatatt cgttttcgtg gcgaatcgct gcttcatgcc agcgatcaaa
    17281 cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg atggtgtcgt
    17341 taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc caccgcggga
    17401 tgcgtcggga agcggctcga ggcgaaattc ttaactgcct tgatcgcgtt ggtatccgcc
    17461 aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa cgccagcggg
    17521 tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat gaaccgacca
    17581 ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa ctgcaaggcg
    17641 agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga aaactggccc
    17701 accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaattacgcc gctacgctat
    17761 ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg tcaggcgatc
    17821 cagtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt caggttgcct
    17881 tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg gtgaaaaaca
    17941 tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc ggttccggga
    18001 aaagtacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc atcatctttg
    18061 acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt catcgcattc
    18121 aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt ttgcagatta
    18181 ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc gaacaacaag
    18241 tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt tatccggcgg
    18301 agttctctgg tggtcagcga caacgtattg caattgccag ggcattaatt cttaagccct
    18361 cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag gcgcaaatat
    18421 tgacgctatt gaaatcattg caacaaaagc tgcaactggc ctatttgttt atcagccacg
    18481 atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa ggggaagtag
    18541 tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat acgcgtcagc
    18601 tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc ggcaattgcc
    18661 acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt tacaaacagg
    18721 cacggctcac cttcacactc cagcaccgaa cacggaactt cgatgtcgct taacgcctgg
    18781 cttagtttgt acatcaccga ccatgcatta tcaccatccg ggctaagcaa tttaagcgcc
    18841 accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt cgaacctgcg
    18901 aaaccagcag accagcgaaa gttctgcggt agacgatgga cgattgagag ttgtaatgaa
    18961 gtcacttatt ttccccggaa gcacatttac ttcacaattt gtttacatca attttaacac
    19021 atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct gcgtggcctc
    19081 tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc tatatgtacc
    19141 cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt acataaattg
    19201 attttacata aaataaacat atatcggggg aatgatagat ttgtggttga catgcatcaa
    19261 caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc ggtcgactgg
    19321 cgtacagaca gaagagaatg gagctgcttg cgcagaatgc aattgaccaa atcatcggcc
    19381 atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct atgccaaaac
    19441 ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca tcaagaatga
    19501 ccgccatcgc attggatgac accatcgaca cacagcccac aaacgccgca acgccaacca
    19561 ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa taaattgtat
    19621 ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cgaacgaagc ggctgttaaa
    19681 gatggtcatc acgaacagga aaacaatgtt tagtgcaaag taataaccaa agttttccgg
    19741 cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg ctcaggaacg agaacattcc
    19801 ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac ggaacagcgc
    19861 cgcaaagtta ccaatagtag tacgaatgtg aaaaggctgg cgacgctccg gtggtaaggt
    19921 ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg cgaggatcca
    19981 gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg gtgccatcag
    20041 cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact cttctttcgg
    20101 gtagatatca cgcatcaaag cgttgataac catgctggcc gcagccgcag ccagcccgtg
    20161 gaaaaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg ccaccgcggc
    20221 ggcggcaaac accagcgtac cgccgagcac caccggctta cgcccgaagc tgtctgccat
    20281 cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactga gggtcatctg
    20341 cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg gcagatacat
    20401 atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa tagcaaacga
    20461 cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt agacgacgct
    20521 ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag catccagcgt
    20581 aataccgcca atacgttcac gatgcagctc aaccacgtgg ttacccacgg cggcgaacat
    20641 gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg ttggggtaat
    20701 cacttccagc accgcaggct tagtgagatc tttttcgtta tgcagctgca cgcctttagc
    20761 aaattgctct gccgtatcgt cagctacagg tgattccagt gtcaccagat aggtcttctc
    20821 gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatcag tcatcagcac
    20881 cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc agtttccagg ctaccggttc
    20941 atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa catagccctg
    21001 aggcttattg agcatgaagt aacgtggacc atgttgctgc gccagcgggt tgccatcgta
    21061 agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cgaacaattt cgccatcgac
    21121 ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc cgagttgctg
    21181 tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaacgg aggtcgggca
    21241 ttgacccgaa aatctgaact gttgtctgcc cagtatagcg gtctaataac atccctcaag
    21301 ggaaaaagat ccatggcata ctattagcag aataatctac ctacgcgaga ccatgatttt
    21361 tacactccgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca
    21421 taaaacccct gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg tgattgccga
    21481 actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca cacgtcaaag aactggtggc
    21541 gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg ccgctgggtt
    21601 aaagcgcaaa gagagccacg gtaaagtggt atttggcagc gtgcagtcgg tcgctcgtaa
    21661 tcttgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgcc accgtattgg
    21721 tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgactaaag tgaatcccca
    21781 cttacgcctg ctggggctga ctgccacgcc ttttcgattg ggcaaaggct ggatctacca
    21841 gttccattat cacggcatgg tacgcggcga tgagaaagcc cttttccgtg actgcattta
    21901 tgagctgccg ctgcgttata tgattaaaca cggctatctg acgccgccag aacgactgga
    21961 tatgccagta gtgcaatacg atttcagccg cttgcaggca cagagtaacg ggctgttcag
    22021 cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc attacgccac atatcatcag
    22081 ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg attttcgccg ccaccgttga
    22141 acacgcaaaa gagattgtgg gattactgcc tgccgaagat gcagcactga ttactggcga
    22201 cacccccggc gctgagcgcg atgtgttaat tgaagatttt aaagcccagc gttttcgcta
    22261 tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc
    22321 cattctgcgt cctaccgaat cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg
    22381 tctcgctcct ggcaagactg attgcttaat tcttgattat gcgggtaatc ctcacgacct
    22441 ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgtcccgg tacaggtttt
    22501 ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg acgggacatt
    22561 gattgaacac tttggtcgtc gctgtcaggg atggtttgaa gatgacgacg gccatcgcga
    22621 acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcgg aaaacgatat
    22681 tgccgcccgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg atatgttaaa
    22741 agcggcgcta cgactgaaag acgcgctggt attacgctgt agcggcatgt ctttacagca
    22801 tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag acggcgcgga
    22861 tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga actgccttcg agcagctttt
    22921 tatccgcccg catacgcgca caccgggcat ccctctgcgc tggatcaccg ccgccgatat
    22981 cctcgcccag caagccttac tgcgacatcc ggattttgtc gtcgcccgca tgaaaggtca
    23041 gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga
    23101 attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata gaatctcgcc
    23161 cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt agcaggatta
    23221 atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag cagggtaagg
    23281 gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac ggtggcaaag
    23341 aagcaccgct ggctatcgag ctggatcacg acaaagtcat gaacatgcaa gctaaagctg
    23401 aattctacag cgaagttctg accatcgttg ttgacggtaa agaaatcaaa gttaaagctc
    23461 aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc gttcgcgctt
    23521 aattgctgaa taagttgtaa aaaaccccgc tccggcgggg ttttttgtat ctgcagatta
    23581 tgcctgatta cggtattgct attttttgca ggccagataa ggcgttcacg ccgcatctgg
    23641 cattcagaat acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa ttggtcgcgc
    23701 aaattcggcg gcgtgccttt aatggtcaag gtatcggttg ctggatccca gaagatgcgt
    23761 tcacctaaca gcatcgcatc aaagttaata gtcaagccac cgccgctacc ggcaaacttc
    23821 gtcaactgac gcagtgtgct acggtctgcc gggaagctct cttccagttc gtagcctttt
    23881 tcagccgcaa acttcgtgaa gctcacttcg cttacgcctg ccagctcttt cgacagcgat
    23941 tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata aacctgctgg
    24001 cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct
    24061 tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt cgctggcccc gaggaaatcc
    24121 ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag atagcgggtg
    24181 gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc cgcatgattg
    24241 atatcaagat aatgggttgg gttgatgtcg agattttcgt taacgcgcat actgctcagg
    24301 ttgctcagaa ctgccaccag cagatactcc accgccagat aacggtagtg gcaaaacagc
    24361 acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg cccggttgcc
    24421 gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc
    24481 agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata gacccgatgc
    24541 agttctgcca ccatctcaac gacggtttct gtcggttcca gcaatgaatc gcgcaacacc
    24601 agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg gttgatatcc
    24661 agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc ggttaagcga
    24721 cgcaataaaa gatggcggtc attttacaga aggcagcgta acgagaatga tttaacgcat
    24781 aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc aactaactga
    24841 tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca attgctcgca
    24901 gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct gatggtgtta
    24961 ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg ccaggcaatt
    25021 gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc gcactaaggg
    25081 aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag tctcccagat
    25141 ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg tcattggcag
    25201 ccgttacctg tttatcgccg actggccgac aacgcttgct ggtcgcattt attcctacgt
    25261 aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga tcctcttccc
    25321 gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca ttctggcaac
    25381 ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc atctccatct
    25441 taatcccatc gtctggcagt tggttatcaa cccagacgaa aatgagatgg cgcgcgactg
    25501 gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt ttgcgacgtg
    25561 gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc cgctggccgc
    25621 attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg atgccaactt
    25681 ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg
    25741 acgtttcctt gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc gtcttattga
    25801 gcaaggtaat ccagacgccg tttccgtaca gtatccgtta agcgaactgc gctatcgcga
    25861 tatgggcacc gggcagaatg tgctgttgat tactgtcgat ggcctgaact actcacgctt
    25921 cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca
    25981 tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg gcatctcgcc
    26041 gagctatatg gacggcattc tgtcgacccg tacccctgcg gcgttaatta ctgcgcttaa
    26101 tcagcaaggc tatcagctgg gattattctc gtcagatggc tttaccagtc cgctgtatcg
    26161 ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc ccccaatccg acgagcagac
    26221 cgccacgcag tggatcaact ggctgggccg ctacgcacaa gaagataacc gctggttctc
    26281 gtgggtctct ttcaatggca ctaacattga cgacagcaat cagcaggcat ttgcacggaa
    26341 atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca atgcactgct
    26401 tgattctggc aaactggaca atacggttgt gattatcact gccggtcggg gtattccgct
    26461 gagcgaagac gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc cattagtgat
    26521 tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata ccgatctgat
    26581 gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat attcgcaagg
    26641 tcaggatttg ttcaaccctc aacgccgtca ttactgggtc actgcagcgg ataacgatac
    26701 gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta aataccgcac
    26761 ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt tgttattgca
    26821 agtgctgaca gacgagaagc gttttatcgc taactgatta attataaatc agttagcgaa
    26881 atatcttact tgcaatcggt gtggaaaacg gtagtattag cagccacgag tcggcacgta
    26941 gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt
    27001 gccgaccaaa tcgcccaccg gataccaccg gaattaacag tttttagaca cgaaaagccc
    27061 gctaatcctt aattagcggg tttttttatg gtttttagtc accggacatc accggattta
    27121 taagctatca gccggatttt ttagttatac gcttagttat acgggcttgc gtataactaa
    27181 agccgtataa ctaaaaccct gttttttcgt gtggaatcat atacatatgg cgagaaaaac
    27241 acccccatta accacggtgc agatcaaagc agccagacca gcggaaaagg aatacaccct
    27301 acaggacggc ggagggcttt ttctcctggt caaaccgtcc ggatcaaaac tctggcgatt
    27361 ttcctactac cggccatcgg acaaaaaaag aatattgctg agttttggat cgcttgatga
    27421 tgtttccctg gctgatgcca gaaaacgccg tagcgagtac agagcgttaa tcagtgccgg
    27481 aactgacccg cagggccatg agaagaaaaa acgagaggca gaggcccgaa gacaagggaa
    27541 cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa atcagccaga atctggcccc
    27601 caacacgatt aaagacatct ggcgttcgct ggataaatat gtattcccgt tcatcggcaa
    27661 cacgccaata gataccctca ccgcccgaag gttcgttgaa gtgcttacac ccatcaagga
    27721 gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc gttaatgagg taatggatta
    27781 cgccgccaac agtgggctga ttgatgccaa tccggctatg aatgtgcgta aggcgtttcc
    27841 ctcacctgta aaaaaacata tgccaacaat ccgccccgaa cagctgccgg agcttatgca
    27901 ggctttatca gtatcggcaa cagaacggca gaccagatta ctgattgaat ggcagttact
    27961 gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg gatgaaatca acctggacgc
    28021 gaagcaatgg acgatacctg ccggacgcat gaagatgcgc agggatcacg ttatcccgct
    28081 ttccggtcag gctatggcgg tgctggaggc catgaaacca atcagccacc accgcaatta
    28141 cgttttccca agtctgaaag acccacagca gccgatgaac agccagacag ctaacgcagc
    28201 attgcggcgt atgggattcg ctggcgtgct ggtgtctcat ggattacgcg ccatattcag
    28261 cacagcagcg aacgaggaag gattcgagcc ggacgtaata gaggcggcac ttgcccacgt
    28321 cgacaccaac gaagttagac gggcatacaa ccggagcaac tacatagaaa aacgcatcgt
    28381 gctgatgcgc tggtggggcg aatttgtcga ggctgcggcg acgggcgtaa ccctcgccag
    28441 tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa ccagtaaaaa ctacgaaaac
    28501 catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca aggccgtgaa acatgaaacc
    28561 gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc tgcaactggc cctgatggtt
    28621 cagcatgaat tttggtcaac ctacgatccg gaggacagaa cgacggcccc atcaaaaaaa
    28681 caggtagtag attttctggt atcccgtggc gcttccagaa atctggcggt aagtattgat
    28741 aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc ccaaaaaatg gcggtaacaa
    28801 tcctggaagc ggcagaaatg ccgctttttt tataattcca tttcaaatca tcaatataaa
    28861 aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg atgatttacc
    28921 gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag caatcagaaa
    28981 aattatcttt cgccaggaag taaaaaaaat tatccacatc aaggcagaca gcacgctgca
    29041 aagcatgatc aacgccggag aatttccgca gggttttcgc gttggtttac gccgtcgcgg
    29101 atggtatgag gatgatgtgt tggcctggca gaaagaacgc gaacaggaag cacgcggaac
    29161 ggctgcttaa cgggtatcac agatatgaca aacacgaaaa aagcggcccc gatatggagc
    29221 cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat atgatcccaa acataagcac
    29281 ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc agttcgttgc
    29341 catacctgca atgagcgctt ttccctatac tctttaagga attgctcaag ggcaaaagca
    29401 catggtgcga atctttctga ttcatgctct atctttctgc gccgtctttt ccgtgccggt
    29461 gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc aatgcgccgt
    29521 aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc gcgccgcttt
    29581 agttcagtgc gccccaactc tttaagccag ttggctaggc ttatgccgtc gctctgtgct
    29641 tctttgtcga attgctcttt tagctctgga gaaattcgca ttctgaattg tggggattgc
    29701 ccgtcccctt tagggctttt atcgcgtttg attattgaca agtggtcacc tattgaatta
    29761 gcctttccat tgttaggtgg ccaccttaac acaagagcac ttaaaaaagc aaagccccgc
    29821 aagtgtcatt accactcgca gggcttctaa ccaacaacgt aaactaggag ccgttatggt
    29881 tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt ggcgttttct
    29941 ggccctgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg aacgcgaagc
    30001 gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc gtcaggggga
    30061 aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact acgctgatgc
    30121 acgcgcttat tgatattgaa tgcactgcag agcttgcgca gggagaagaa cagaaagacc
    30181 ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcgctg aatgacgcta
    30241 aaaatattct tgttgctgat tgtgaaaatg gggggggtta tgcgtgatga tcgttttaat
    30301 tccctgaaac aggaatttcc cggcgttcct gatgatgcgg ctgatgcgct ttcgtcaatg
    30361 ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa atcaacgggg
    30421 cttgatgtac tgaatatcgc cgccgattat gcggaatatg tggcagaggc gcgttacaga
    30481 agaaaatttc ctgaggatgt aagccatgcg tgatatttac cacgaaacaa tagaccgcgc
    30541 atttcttgca ctttctcaca gtgaaaacat gctggaaata ttgcgcatat ggcttgaaac
    30601 acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gccacggcat taataacgct
    30661 tcttgagcct gtaataatgg aactgcaaga aatagatcta ttgcacgaca gatataaaga
    30721 acagcacacc ggagaataaa aataatgaaa cttaaatatt ctggcttaac tgccagtggc
    30781 aacactcacc ctaaatttac gcgcggtgat atttaccgcg accagtacgg cggcacggta
    30841 atgattaagg gcgtggaaga acggcgtgta acctaccgcc gtgaaggtta cgaatatgat
    30901 tgcgtgatgc ctgtttatca gttccggcgt gatttttctc tggtacagac cgcgccgcat
    30961 aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc agaagctgaa aaccatgatt
    31021 aacggattca ggggcaagaa atgaaaagcg caccgaactt aaaaaaacag ccttacgaca
    31081 agatgaccga agtcattatt tttgcgggta gtgatgcctg ggcacatgcg aaacagtggc
    31141 aggaacagga cgggcgactg gctggcgata atgtgcctcc cgttgtgctg gctgatgatc
    31201 aactggatga actggcagac ctgagaatca tcgacgaggg gcgctattgt gtccggctgt
    31261 acaaggcagg ccacatcagg ccatcaaata ttaatgccat tgcgcacaag ctggcggcgg
    31321 cgggtgtaac tgatgcgaat tattaccccg aagggatgca cagccatatg cgggagaact
    31381 ggcgcgaata cctggaacgg gtgcgcggga aagagccggt ggaagaaaaa aaccaccagc
    31441 gaaaaaccac gctaccgatg agcgttggat ctaccggata cgacacgcaa ctggattacg
    31501 tggttaaggg gattattccg gcggtatcgc tatgcagcat atacggggct agcgggtcct
    31561 ataaatcatt ccttgccgga tcgtgggcgt gccatgttgc cactggtcgc cagtggggag
    31621 gccgcagggt tgcacatggt gcggttctct atgtggttgg tgaaggcggt atcggcgttc
    31681 cgcgtcgtgt aaaagcctgg gaggttgtgc acgatgagca ggtgaaaaat ctgtatctgg
    31741 taaaccgccc catctttccg gctgccccgc ttgatgttga tgaaatggtt atcgctgccc
    31801 gtcaggtgga gcgggaaacg ggtaaacctg tacgcatgat tattctggat acgctggcgc
    31861 gttgctttgg tgggaatgat gaaaatgatt cccgtgatat gggggcgttt atccgtggtt
    31921 gtgacgaact gaaacgacgc acaggggcca cggtgctggt ggttcaccat tccggcaagg
    31981 atgagacgaa aggcgcgcgc ggttccagtg catttcgtgc ttcgctggat gctgaatacc
    32041 ggatacgcag ggaggacgca ggaagcgaag cgctggttat ctcatgcacc aaaatgaagg
    32101 acgcggagga actcaaagaa gccgcatatg acttacgcgt ggtggagctt tttaccgacg
    32161 ctgacggtga attaatcacg tcgctggtgg tggtggatga tccgcgccct cctgttgaac
    32221 tggagcgcat cgaggaggca gggaacaaga cggaaaacca taccgcgcta tgggggtgca
    32281 tccgttcacg cacacagaac ggcgacaagt gcacgatccc gctgttacgt gatgacatga
    32341 aaaagctggg gtatgaaatg aaaaacttcc ggcgctggct gtacaagctg gaaaaagatg
    32401 gggttattag tatcgatggg gatgatgtag cgccgctata aaagtgagga gcaaaagcga
    32461 gggggataga aagagggcca aaattagccc gctctccctc acttttcgac ctgtatacat
    32521 cctcaaaagt gaggggtaaa aaatacttat aaaacacaca catagaaaaa ccgaaaatcc
    32581 caactgcgac gaagtgagac gcttgaaaaa gtgaggcgaa aaagtgagag gttgcgagaa
    32641 atgacccaaa aacgcagaga cagaacagag ccaaaatata aagcgttaga catgactgag
    32701 cacaccttaa aggtggcaat cagaacgata gaccgccaca cgcgggaagg atacgcgaag
    32761 gaacatcccg acctgataag cgcattcatg accacggcgg cggcaaactt tgccacgctg
    32821 acagaacggg agattgccga agcggaacag gtaacaacca tcaacgttaa aaccggagag
    32881 gtggaattat gacggcacag atagccgctt acgggcggct ggtggacgac ccgcaggtaa
    32941 aacagaccag caagggcaca ccgatgacgc tggcgcgtat ggcggtatct ttgccatgca
    33001 gccaggcaca ggacggacag gcgacgttat ggttatcggt catcgcattt ggcaaacagg
    33061 ccgacttcct ggctaaacat caaaaaggcg atgttgccag cgtatccggc acgatgcagg
    33121 taagccagtg gaccggacag aacggagaaa cgcggcaggg ctggcaggtt atcgcagaca
    33181 gcgtaatcag tgcccgcgcg gcacgtccag gcgggaacag acgcaaaacc acaggcacac
    33241 agggtaatca gccaccagcg ggaggcgatg acccttacgg tgatgatatt tcgttctgag
    33301 ggggtgacga tggtacatga ccgcatagcg gaggaactgg aggcgaaagg cttttaccgg
    33361 agggcggcgg cgcgatgggg tgaagtcatg cagctggtgg agacagacaa ggaacggcat
    33421 cacatcacga tgcgacggct ggaatgttca aggaaggcac agagggcacc ggagccgccg
    33481 gataatttcg gagacctgaa aaaggcagtc gatcgcactt atgccgaaat gggtatagat
    33541 ggtgctggtg atgaaatatg gcgcaattac caggacagct aatcaaacag ccggagaaat
    33601 ccggcttttt tgcgcccaaa aaaagcccga taaggtcaga gggttcttat cgggcttttg
    33661 catatgaggt ttttggatgc actggcgttc gtgatcggga taatcatttc ataatttgca
    33721 acataactca atattattgc ataaaatgca attctgatta taatcaggac tggataaaca
    33781 tccagttatg atttttttag tcgaagagga atttcttact atggctgaag agaaaaaagg
    33841 cggtgtttcg gtgtacataa gccccgaaat cgtggaggtg ctcaagcagc gccacaaaaa
    33901 aaactatgag gctggcgtgg cggctggact ggatccgctg atgacgccgg agccgtcgat
    33961 aggttcactt gtacgctctt atttacttgc ggcgcttggg atgcataaaa attatggggg
    34021 tgaataatgg caggcaaagc aacggcactt aacactaacc agctttttat gtacctgaat
    34081 cgcggggata ttgcggattt taaattcagc cctctgttta ccacgctgtt tttcccgaac
    34141 gtggcgacat tcagcacgca aaacatcatg ctggataccc tggacattga agaagtcacc
    34201 atgtcggcgt tttgttcgcc tatggtgggt agccaggttc agcgcgataa agggtacgaa
    34261 accagcacaa tcaaacctgg ctacatgaag ccaaagcacg aaatcgatcc aacgaaaacc
    34321 atcatgcgca tggctggaga agatccggca cagcttaacg accctaccta tcgccgtatg
    34381 cgcctgatta ctggcaacat gcgccgccag ataaacgcca ttaaggcacg cgtggaatgg
    34441 ctggcggtga atgcggtaac gaccggaaaa aacatcattg agggcgaagg catagaacgc
    34501 tatgaaatcg actggaagat accggaaaaa aacatcatag agcagtccga cggcaaaaaa
    34561 tggtccgagc atgataaaga tatttacgat ccaatctatg acatcgaact atacgcagat
    34621 caggcaggtt gccccgccaa cgtcatgatt atgggcgctg aggtatggcg cacgttacgc
    34681 agctttaaaa aattccgtga actgtacgat ctttcccgtg gttcagaatc cgccgcagaa
    34741 atggcctgta aaaacctagg cgaagtggtg agctttaaag gctatctggg cgatattgct
    34801 cttattgtct attccggcaa atacactgac agcgacggca ccgaaaaata tttccttgag
    34861 cctgatttgc tggtcctggg caacaccaac aataaagggc tggtggccta tggtgcgatt
    34921 atggatcagg aagcggtaag aacgggcgca acgcaaaaca tgtactaccc gaaaaactgg
    34981 attgaggacg gcgatccggc gattgagtac gtgcagacac acagcgcacc gcagccggtt
    35041 ccggcagata ttcgcaaatt tgttaccgtc aaaattggtt aacgggggat tatatggaca
    35101 ctccatatat tgagttattt gcaggcagtc agcaggtatc aacgacactg gtacattttg
    35161 ccgctgatgc tggcgttatt caggaattta ccccgctgat gctggtggac aatggcgagt
    35221 ttaagccgtg ggatggtcag gaatctggca aggctgttta tctgacttcg tacctcgtgg
    35281 acacgtcaaa gcagaaatca gcacagtgtt acaagacggg gatattcaat attgccgccg
    35341 ttaactggcc tgagagcgtc gacaccgatg cgaaaaaatg cgccgccttt gcgggttctg
    35401 gcgtatccgt tcagccgctg gcccgataag cagggggaac gatggcaacg aatgaaagca
    35461 tcatggcgct accgctggcg agtaaattta aagccgaagc gcgggcaatg gctgacagag
    35521 gtttatcaac ctacgaggcc gtatatcaac tcaacaaact ggaagagcag gacaagccgc
    35581 gcgctgatgc gattatggcg cttcatgagc ataaagacta tcagccgctg ttgcgtgcaa
    35641 tggcaaacgt gccttgtatc gatgttgata cggctaaaaa catcctgaac atgaccatag
    35701 agcaggaacg cccgaaggtt gcaccagagc ttaccgcagc ctttgaaaac tttatggaca
    35761 tgcacagccc acaaaccgta tcagctggca tggcatacga tggcagaaac ccaggcgatg
    35821 acggcgacat cgatcgcata ctgaaaacca tctgagacaa ggccggagaa atccggcttt
    35881 tttgcgggtc cttcctggaa ttatggcccg ttacggggcg gcgacctcgc gggttttcgc
    35941 tatttatgac gtttttccgt gaaggtgaca ccaccaccac ttgattaata tttaaccatg
    36001 cagttaaggt aacattatga ttgataaagc ttgttttgta agtcagcagg aaatagctga
    36061 acatttcaag gttaacagaa ccactattcg cgcatggacc aaacagggga tgccgtatct
    36121 taatgcggat cgcggaaagt ctggcggtta tcacatcggg catacattgc tttggtcttc
    36181 aggtaaaagc cgtcttgagg ccatcagata tcacgtagaa accagtgcgc tggaaaaaat
    36241 tatgtttgcc aggctgcttt catctgagcg tgacgagtac tccagtgaag aaacagaaca
    36301 tcgatttgat gaaggtttgc agatttacgg ctattcaccg gaagatgtga gcaaggcacg
    36361 aaataaaatg gctggctttc tggctgggtg gcgtcatgcc gtaagcgttc gccgtgccag
    36421 catggagcaa tcagccgata cagaacagta aagccgataa cttgttaatc actcgcagca
    36481 atgcatgtga tttgtgcaca ctttcggtta actggttcga tgtccggttt tagtgtctgt
    36541 tttttgcgca tgtccggttc atggaaagca tgtttttata tttttcatat ggttaacttg
    36601 tagagaaacc ggacatggat cccgaaaaat tttcataaat agtgaaaacg cgcgaggtcg
    36661 ccgccccgta acgggtccat aattccagga aggacccgac gacaccagac aatcagaacg
    36721 ataggtgcac aatgacagaa gccgaaatac tgggattaat ccgccgtgct ggtggaatca
    36781 gccagcagac tgacgaacag gccacgcagc cggacagcgt gacagccgaa aattatgcgc
    36841 gtgttgttgc tgaggtgatg cgccgtgatg gtatccagct taatgatgtg gatatgcgca
    36901 acatacggat ccgcgttctt gaaatgctgg cctacaatcg ccgcgtggca ctgtatcggg
    36961 aaacggagaa aattacatac cactggaaga agccggagcg gttgcggcgg taactggttg
    37021 atattttcga aattcgccag cggcgaacat cggggaaacc agcctaaccc gttgatattt
    37081 tcgggaacct caaattgagg aggtcggaaa cataacccgt catattctgg atgctgataa
    37141 ttaccacgca gcccattaag gcctaacccg ttgattcttt cggaaacagc cattggcgta
    37201 atccggcgcg gtaacttgct gaacttaaag caaagcgcaa aattgcgctg gctggtgggc
    37261 atgatgccga tatggtgatc cccataacgg aaatatccac cagcacggca acggcagaat
    37321 agccacaaca cagaaaaacc acgaatatgg ggtttttgtt atggcatggg catgatgacc
    37381 gcccctgctc tgaatggtgg aaatcaccac gcagatcatc caccagcttc gtttttataa
    37441 attaaacatc cgggaaaatc cgaccctgaa ttgtgcattt tttcagcaaa tcattttagt
    37501 tatacgtttg gttatacggt ttaaaagttg aatcatatac attattttaa atcatgaagt
    37561 tacacgctca attaaactac tctcgtgcaa aaatcccaag aaaaaaccaa cctttacggt
    37621 tggttttttt atatctgcaa ttaatttgat aaacagaccg tgacacatca cagcctgttt
    37681 attttctgtt atcagaacgt ccagaccact cccgcctgag tattccacgg cgattctacc
    37741 cctgcgccat tgccatagct gactgacaaa tgaccgctta acgtcggggt aaacgatgac
    37801 cttatcccag cctgataaac gccaggggtt ccagccacat cattattaaa tttgccatcg
    37861 tcattaactt tcacatggtt agaatcggcg tattcctgac gtacggcggc tttcagccag
    37921 ggttccagcg tcgtaccatt ttgcaggtcc atgtgatagc ttaccgccgt tcccgcttca
    37981 gcgcgtaata tccgggtatt tcccacatca gcgcgcatgc cgtttgataa cgtgtagtcc
    38041 tgaccatctg tggtaaagcc ggtaaaggcc agatagggtc taacactcca caatccgtca
    38101 acccaacgga acccgctctc aacatgagca cccgcgccgt tactattgta atcgccaaac
    38161 gctgttgccc cattactcat cttgccatgg atggtgttgg caaaacggtc aactttcacc
    38221 accccatcaa cataggcacc gttctgatgc tcccaaccgg cataagcccc cagggtatag
    38281 ctatcgatat taccttttcc gccgcgatca aaaccaatat cagaatgaga gtaaccaaag
    38341 aacaagccgc gaattgtact gctttcttca cgggagaaac ggctatcgat accgagcgtc
    38401 aggcccgtca atgtttgctc aaaaccagct cccgcatcag tggtcacgtt gttgcgggtg
    38461 ttaattgccg aactccacat cgccgtatcg taactaacgc tttttacgct accaagacgc
    38521 tcacgcacgg tgtccagttc tgcatcaaat accagcggtt gtgcggccgc catattcagc
    38581 acatcagtgg ttgaaggggt aatttgcgcg cgattctctg ccaaactcca gctatggttg
    38641 ccattatcca gcaaggtata ttcatacgta ccgatatcaa caacgcctcc ggcattgccc
    38701 aacgtaaatg cagcatcacc gccgcccgtt gttaccagtg taaggctatc tcctgctgcc
    38761 gggctggcac cggtgtccgt cacgaatatt ttgaaatcac ctgttgcctg accggtgacg
    38821 ttgagctgat cgctctgatg attagccata tcggtacgca tataaaaatt gccgtttccc
    38881 gacagggtat tggtggtcaa cgtaatgaaa ttttccgcac ttgccgtcac tgatgaacga
    38941 gtcactggct catccatcat aatcacattt gcgtcagaca tcgcaaggtt ggctaagttg
    39001 aggagggtgt tttggttcgt cgtagtgata tcggcaatga tggcccatac gctattttcg
    39061 agcgaaacgt ccgctttgct ggtatccacg gcaccatgcg ttctaaaaga agcgccctca
    39121 gaaaccacta ccttacccac caacatagcg ggtgcataat ttatttgcgg cttcatgacc
    39181 tcaagaatgc catcattccc tctgactgac acgttaacca ttgtgccagc ccagacatta
    39241 gcgcggccat tgttgataac catattttca gagatggctt tagaggaata cgtgatactt
    39301 ccattctggt ctgatcgacc gagatcatat accgcatttg cctgcacttt cgtaccagta
    39361 tcctttccca gcgattgcat agtggcatgc tcatcaagga tagtatcaat ggcctgcgtg
    39421 tcctccataa caatgagccc ggaaccatca tccagttcat aattttttga cacaccatct
    39481 tttatactaa attggccttt actgtttgta ccactcactt ccagcgcatt cgttgagaca
    39541 attaattttc cgcctgcttt tttatcaacg ccagttgcag tcccgccatc cataacctcc
    39601 agtaagccgc cactatctac agtagtatta taagcgaagt cattttcttc aactcgcaaa
    39661 cttccgccgt tttccaacag catattgtta gcaataccat tcttgatttc gaactgaccg
    39721 agacggtttg ttccgaatgc ctccatgacc cgcgtgcttg ctttaatagc accgcctgct
    39781 ttctgatcta ccgcaatcgc taatcccccc tctttaacct ccagtaatcc tcccttttcg
    39841 atagcggcaa ggacggtaat cccatctttt tcaacgatta agcgcccgcc tttaatcgtg
    39901 ggatcattcg c
//
LOCUS       DAJVOY010000014        39725 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVOY010000014 DAJVOY010000000
VERSION     DAJVOY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.014"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..425
                     /locus_tag="OQL90_000668"
     CDS             <1..425
                     /locus_tag="OQL90_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS1367547.1"
                     /translation="NLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFAVQS
                     GPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKAKLN
                     VEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            508..1410
                     /locus_tag="OQL90_000669"
     CDS             508..1410
                     /locus_tag="OQL90_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCS1367548.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1455..3617)
                     /gene="uvrD"
                     /locus_tag="OQL90_000670"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1455..3617)
                     /gene="uvrD"
                     /locus_tag="OQL90_000670"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1367549.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3701..4417)
                     /gene="yigB"
                     /locus_tag="OQL90_000671"
     CDS             complement(3701..4417)
                     /gene="yigB"
                     /locus_tag="OQL90_000671"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1367550.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4417..5313)
                     /gene="xerC"
                     /locus_tag="OQL90_000672"
     CDS             complement(4417..5313)
                     /gene="xerC"
                     /locus_tag="OQL90_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1367551.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5310..6017)
                     /locus_tag="OQL90_000673"
     CDS             complement(5310..6017)
                     /locus_tag="OQL90_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1367552.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6014..6838)
                     /gene="dapF"
                     /locus_tag="OQL90_000674"
     CDS             complement(6014..6838)
                     /gene="dapF"
                     /locus_tag="OQL90_000674"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1367553.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6883..7077)
                     /locus_tag="OQL90_000675"
     CDS             complement(6883..7077)
                     /locus_tag="OQL90_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1367554.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            7540..7860
                     /gene="cyaY"
                     /locus_tag="OQL90_000676"
     CDS             7540..7860
                     /gene="cyaY"
                     /locus_tag="OQL90_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1367555.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7900..10446)
                     /gene="cyaA"
                     /locus_tag="OQL90_000677"
     CDS             complement(7900..10446)
                     /gene="cyaA"
                     /locus_tag="OQL90_000677"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1367556.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLCGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10833..11774
                     /gene="hemC"
                     /locus_tag="OQL90_000678"
     CDS             10833..11774
                     /gene="hemC"
                     /locus_tag="OQL90_000678"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1367557.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11771..12511
                     /gene="hemD"
                     /locus_tag="OQL90_000679"
     CDS             11771..12511
                     /gene="hemD"
                     /locus_tag="OQL90_000679"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1367558.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12533..13738
                     /gene="hemX"
                     /locus_tag="OQL90_000680"
     CDS             12533..13738
                     /gene="hemX"
                     /locus_tag="OQL90_000680"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1367559.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            13741..14937
                     /gene="hemY"
                     /locus_tag="OQL90_000681"
     CDS             13741..14937
                     /gene="hemY"
                     /locus_tag="OQL90_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1367560.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15616..15777
                     /locus_tag="OQL90_000682"
     CDS             15616..15777
                     /locus_tag="OQL90_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367561.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15861..17096)
                     /locus_tag="OQL90_000683"
     CDS             complement(15861..17096)
                     /locus_tag="OQL90_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1367562.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17243..17319)
                     /locus_tag="OQL90_000684"
     tRNA            complement(17243..17319)
                     /locus_tag="OQL90_000684"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17283..17285),aa:Pro,seq:tgg)
     gene            complement(17362..17448)
                     /locus_tag="OQL90_000685"
     tRNA            complement(17362..17448)
                     /locus_tag="OQL90_000685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17412..17414),aa:Leu,seq:cag)
     gene            complement(17469..17544)
                     /locus_tag="OQL90_000686"
     tRNA            complement(17469..17544)
                     /locus_tag="OQL90_000686"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17509..17511),aa:His,seq:gtg)
     gene            complement(17603..17679)
                     /locus_tag="OQL90_000687"
     tRNA            complement(17603..17679)
                     /locus_tag="OQL90_000687"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17643..17645),aa:Arg,seq:ccg)
     gene            complement(17782..19167)
                     /locus_tag="OQL90_000688"
     CDS             complement(17782..19167)
                     /locus_tag="OQL90_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1367563.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSSGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19358..20098)
                     /gene="rffM"
                     /locus_tag="OQL90_000689"
     CDS             complement(19358..20098)
                     /gene="rffM"
                     /locus_tag="OQL90_000689"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1367564.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20101..21453)
                     /gene="wzyE"
                     /locus_tag="OQL90_000690"
     CDS             complement(20101..21453)
                     /gene="wzyE"
                     /locus_tag="OQL90_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1367565.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21450..22529)
                     /gene="rffT"
                     /locus_tag="OQL90_000691"
                     /gene_synonym="wecF"
     CDS             complement(21450..22529)
                     /gene="rffT"
                     /locus_tag="OQL90_000691"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1367566.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(22526..23776)
                     /gene="wzxE"
                     /locus_tag="OQL90_000692"
     CDS             complement(22526..23776)
                     /gene="wzxE"
                     /locus_tag="OQL90_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1367567.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23778..24908)
                     /gene="rffA"
                     /locus_tag="OQL90_000693"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23778..24908)
                     /gene="rffA"
                     /locus_tag="OQL90_000693"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1367568.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24913..25587)
                     /gene="rffC"
                     /locus_tag="OQL90_000694"
                     /gene_synonym="wecD"
     CDS             complement(24913..25587)
                     /gene="rffC"
                     /locus_tag="OQL90_000694"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1367569.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25565..26446)
                     /gene="rfbA"
                     /locus_tag="OQL90_000695"
     CDS             complement(25565..26446)
                     /gene="rfbA"
                     /locus_tag="OQL90_000695"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1367570.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26465..27532)
                     /gene="rffG"
                     /locus_tag="OQL90_000696"
     CDS             complement(26465..27532)
                     /gene="rffG"
                     /locus_tag="OQL90_000696"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1367571.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27532..28794)
                     /gene="wecC"
                     /locus_tag="OQL90_000697"
     CDS             complement(27532..28794)
                     /gene="wecC"
                     /locus_tag="OQL90_000697"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1367572.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28791..29921)
                     /gene="wecB"
                     /locus_tag="OQL90_000698"
     CDS             complement(28791..29921)
                     /gene="wecB"
                     /locus_tag="OQL90_000698"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1367573.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(29977..31023)
                     /gene="wzzE"
                     /locus_tag="OQL90_000699"
     CDS             complement(29977..31023)
                     /gene="wzzE"
                     /locus_tag="OQL90_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1367574.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31035..32138)
                     /gene="wecA"
                     /locus_tag="OQL90_000700"
     CDS             complement(31035..32138)
                     /gene="wecA"
                     /locus_tag="OQL90_000700"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1367575.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32378..33637)
                     /gene="rho"
                     /locus_tag="OQL90_000701"
     CDS             complement(32378..33637)
                     /gene="rho"
                     /locus_tag="OQL90_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1367576.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33722..33823)
                     /gene="rhoL"
                     /locus_tag="OQL90_000702"
     CDS             complement(33722..33823)
                     /gene="rhoL"
                     /locus_tag="OQL90_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1367577.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(33964..34293)
                     /gene="trxA"
                     /locus_tag="OQL90_000703"
     CDS             complement(33964..34293)
                     /gene="trxA"
                     /locus_tag="OQL90_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1367578.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34424..35689
                     /gene="rhlB"
                     /locus_tag="OQL90_000704"
     CDS             34424..35689
                     /gene="rhlB"
                     /locus_tag="OQL90_000704"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1367579.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEILEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35823..37307
                     /gene="gppA"
                     /locus_tag="OQL90_000705"
     CDS             35823..37307
                     /gene="gppA"
                     /locus_tag="OQL90_000705"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1367580.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37354..39375)
                     /gene="rep"
                     /locus_tag="OQL90_000706"
     CDS             complement(37354..39375)
                     /gene="rep"
                     /locus_tag="OQL90_000706"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1367581.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9861 a        10792 c        10043 g         9029 t
ORIGIN      
        1 taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg ttttatgttg
       61 cgggagataa agtcggcatc attcgtctgg atgcctttaa aaccagtaaa gagattcagt
      121 ttgcggtgca gtcagggcca atgttattag aaaacggcgt aattaatcca cgcattcacc
      181 cgaatgttgc ctcgcgtaaa attcgtaacg gtgtagggat taataaacaa gggaacgccg
      241 tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat gccaaagcga
      301 aactgaacgt tgagcagttg ctttatcttg atggcacaat ttcacatatg tatatgaaag
      361 gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc gagcggaaag
      421 gttaagcatt gtacactcga ggaggccaga caaactggga ctggctacac agctggaaaa
      481 tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt atttttatta
      541 acgtgtctgg gatcatcatc cgcctatgcg gataacgctt tgattttaca aactgatttt
      601 agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggcgt tgaccataat
      661 cttaaaatct ttgatttaac acacgaaatt ccaccgtata acatctggga aggcgcttat
      721 cgcctgtatc agactgccag ttactggccg caaggttcgg tgtttgttag cgtggtcgat
      781 ccgggcgtag gcactgaccg taaatcggta gtgctgaaaa ccaaaagcgg tcaatacttc
      841 gtttcaccag ataacggtac gctcactttg gtggcacaaa ctctgggtat tgatagcgtc
      901 cgggaaatcg acgaaaaagc taaccgcctg aaaggttctg aaaagtcata taccttccac
      961 ggtcgtgacg tgtatgccta caccggtgca cgtctggctt ccggggcgat tacgttcgaa
     1021 caggtggggc cagagcttcc agcaaaagtt gtcgaacttt cttatcagaa agcaaaagca
     1081 acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg caacgtctgg
     1141 agcaatatca gcgacgagtt gctgaatcag gcagaaatca aactcaacga cacgttgtgt
     1201 gtgaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta tgtcgcaagc
     1261 ttcggcgatg tgccggaagg ccagccgatg gtgtatctga acagcttatt gaatgtttcc
     1321 gtggcgctga atatggataa tttcgcgcag aaacatcagg ttgcgtctgg tgctgactgg
     1381 aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg cggaatgcgg tgtggcgcac
     1441 cgcatcccgc aacgtcacac cgtctccaac cgggcataag ccgccaccag ccatttaata
     1501 ccctggccct ggaatgccac ctgcaaacgg ctatgttcac cgctgccttc catattgacg
     1561 atggtgcctt caccaaactt agcgtggcgt acacgctggc cgagcttgta gccgctgtcg
     1621 ttctcgacca tcggcgtacc catccgctga tggctgaccg ggcggcttac cgtggcgcgc
     1681 aggcgcacct cttccacaca ctcttccggc agctcgccaa taaagcgcga cgggcgatgg
     1741 taaacctctt tgccatacag acggcgagtt tccgcgtagg tcagcgtcag tttctgcatc
     1801 gcgcgggtta cgccaacgta ggccagacgg cgttcttctt ccagacgccc gccttcatcc
     1861 agcgacatct ggcttgggaa catgccctct tccataccga cgataaacac ctgcgggaac
     1921 tccagacctt tcgccgagtg catggtcatc agttgtaccg catcctgcca ggtatccgcc
     1981 tgcccttcgc ctgcttccag cgccgcatgg gataggaacg cctgcagcgg cattaaatct
     2041 tcgtcttctt cgttgtagct gaactggcgc gttgccgtca ccagttcctc taagttttcg
     2101 atacgcgtct gacctttttc gcccttctcc tgctcgtaca tggtacgcag gccggagtct
     2161 ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg cagtttcctg cgctaaggcg
     2221 tcgatcagtt ccataaaccg ctgtaaggcg ctggcagcac gtccggcgag ggctttttcc
     2281 tgcaacagtt cacgacatgc ctgccagagt gttaactggc gatcgcgcga tgtctgacgt
     2341 accacgtcca gcgtccggtc accaataccc cgcgttggcg tattcaccac acgctcaaag
     2401 gccgcgtcgt cgttacggtt ggcaatcagg cgcagatacg agagcgcatc tttgatttcc
     2461 tggcgttcga agaagcgcat cccgccgtaa atacggtacg gcatactcgc ctgcaacagt
     2521 gcttcttcca gcacacgcga ctgggcgtta ctgcggtaga gaatggcgca ctcggcaagc
     2581 gccccgccgt tgtcctgcca ggttttgatg cggttaacca caaaacgcgc ttcatcgagt
     2641 tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg cgccatcggt ccacagtttt
     2701 ttacccagac gcccgttatt gttttcaatc agggcgttag cggcgctcag aatattgctg
     2761 gtagagcggt agttttgctc cagacgaata gtttcggcac cggggaaatc attaaggaaa
     2821 cgctgaatat tctccacctg cgccccgcgc cagccgtaga ttgactggtc gtcatcaccg
     2881 acgatcatca ctttgccggt gtcgcccgcc agcaggcgga tccacgcgta ctgaatgttg
     2941 ttggtatcct ggaattcgtc caccaggata ttggtaaaac gttcgcggta gtgttgcagg
     3001 atatgcggct tgttaagcca caactcgtga gcgcgcagca gcagctcggc gaagtccacc
     3061 aggcccgcgc ggtcacacgc ttcctgatac gcctgataca ccttctgcca ggtctgctcc
     3121 accggattac cgtagctttg aatatgatgt ggacgcaggc cttcatcttt ctggctgttg
     3181 atgtaccaca ttgcctggcg cggcggccac tgcttctcgt cgaggttcat cgccttgatt
     3241 aggcgtttga gcaggcgtag ctggtcctcg ctgtcgagga tctgaaaatc ctgcggcaga
     3301 ttggcgtcca tatggtgcgc acgcagcaga cggtgcgcca gcccgtggaa ggtgccgacc
     3361 cacatgccgc cctggctggt gcccattagc tgcccgatac gatgacgcat ctccgccgcc
     3421 gctttgttgg taaacgtcac cgccataatc gagtatggca agcagttttc cacgctcatc
     3481 agccaggcga tacgatgcac cagtacgcgc gtcttaccac tgcccgcgcc cgccagcacc
     3541 agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt tgtcattaag gctgtcgagc
     3601 aggtaagaaa cgtccattgg caccgccgcg taaaaatagg ttgggcggag aagcgcaacg
     3661 ccctccgcca aaaagctggg tatatataca gatttgctga ttatatcagc gaggtcagag
     3721 atgccaaccg ggaaatttcc agatgcggca gtaaacggct gtcccaggtt tgcatcagat
     3781 cgccattttc cggtctgatc caacaggcct gcattccgct gcgaattgcc ccacccacgt
     3841 cagtggtgag atcgtcccca acatgtaaga tctcgccgat cggcacgttg agtttttccg
     3901 cagccagaaa gtacatatcg ctgaacggtt ttgagcgccc gtgcgggcca gcgcgcagca
     3961 caaactcaaa ataatccccc aaaccaaaca gttccggctg ggcgttaccg ttggtgatcg
     4021 ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc gtgagtttgc tgcgggacgt
     4081 cgattcggct gcgccatttg gcaaagttga tcattgctgc gtgtgcgcct gcactggctt
     4141 cttcggcact cagcccggcg tcgagcatcg cttgttcaat cgaacgaaaa cgccagcgcg
     4201 tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc ctggcgcagg cgttgcagat
     4261 cttcattctg gaagctgcgc agcgccggat gataattttg cacaaaggta agcgcctctc
     4321 gctcggtgcg caaaatcacc ggacggttat cgtaaagggt atcatccagg tcaaaggtga
     4381 gcgccgagat gcgccccaaa ggccggtaaa aacgcattat ttcccccgtt tggcgcgtgg
     4441 atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca agatgagtat agatttgcgt
     4501 ggtggagagg ttggcatgac ccagtagctc ctgcacacca cgaagatcgc cgctcgactc
     4561 cagcatatgc gtagcgaacg agtgacgtaa tttatgtggg tgaacgtgat tattcagccc
     4621 ttgttttatg ccccattcgg caaagcgttt ctgcacatta cgcgcggaga tacgcttgcc
     4681 cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac aggtcgcgca aatcaagcca
     4741 gtgctcaatc cacgccacag cgttgcgacc aatcggcagg cggcgctctt tgctgccttt
     4801 ccccattacc cacacttcac cagactccag gtcgaggtgt ttgatatcca gccccaccag
     4861 ctcggaaaga cgcagaccag cgccgtacat cacttccagc attgcacggt cgcgtacagc
     4921 gaggggatcg ttgatatcaa tatccagcag ccgattcata tcgtcgacgt cgatattttt
     4981 cggcagatga cgcggcgctt tcggtgccga aacacctttc gccgggttag ctttgagttc
     5041 gttctggctg accagccagt caaaaaagct acgtagcgca gaaagccgta acgccagact
     5101 tgctgctccc agccctttac ggcgactgcg tacagcaaaa ttgcgcacca tcgtcacatc
     5161 acattgctgc cagctttgca ggccgttttc gctggcaaaa ttgatgatcg cctcaagctg
     5221 acgctggtag ttaagcaggg ttatcgggct aagctggcgc tccacgctca gataacgtag
     5281 gtagcgttct acatcggtgt gtaaatcggt catacgcgtt caatccaacg ctccagaagc
     5341 tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc cttgcccttg ttgatagtga
     5401 ctggcatcgc gactggtaaa cagcacgaca cccaaatcag catcgcttcc cagcatcgac
     5461 atcgccaccg atcccaccgc tttcgcttcc ggtagcacca ccagcagctc tggtccgtta
     5521 agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa tacgcagcgg ttcgaaagac
     5581 tgacggctta atgccagatg agtgtggttc gacggcgcac ctaagcgcca gcgatccgga
     5641 aacaggcgca gactcgcacc tgccaggccg agatcgcgcg cccagcggtg aaagcgcatc
     5701 agcatatcgt cgagactgct ggcggcggtg agactgcgct gcaggtagag taggcgataa
     5761 aacaggcctt cgttggcgat agcctgttcc atcaacagcg ccatgttctc ttccagaaca
     5821 tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca acgaaacggt gccgcgtacc
     5881 ggatgtggca cacgtatcgc ttctactgcg cgcgcattac ggataaaaaa ctcaggattt
     5941 ttaatcagat aatcgacaac cgcccggtca tcaagctccg tgagtgtttc ctgcagttct
     6001 tcccctggtt gcttcataga tgaataaatc cgtcgtagac atgtaccgcc gggccagtca
     6061 tatataacgg gtgacccgga cctttccagg cgatatcaag acgaccgccg gggagttcca
     6121 cgcgtacttc ttcggccagc aaaccttgct gaatccctac tgcaaccgcc gcacacgcgc
     6181 cgctgccgca ggcctgggtt tctcctgccc cacgctcata aacgcgtaaa cgaatatgct
     6241 cgcgcttaac cacttgcata aaaccgatat tggcgcgctc cggaaaacgc tcgtggcttt
     6301 ccagaacagg accaagcgtt tctaccgccg cggtatcgac atcatcgacc tgaatcacgc
     6361 aatgcggatt tcccatcgac accacgccgc ataagattgt ctgctcggcg gcgcgcataa
     6421 tataggtctt ttccgctttg ttagcgcgaa acggcacagc ggaaggttcg aagttgggtt
     6481 cgcccatatt tacgcggacc agatcatcat cggtgacggt cagaaccatc cgcccgttgg
     6541 cggtgctgac gcggatatcg cgcttattgg tcagcccttt caggcgcaca aaacgggcaa
     6601 agcagcgcgc accgttgccg cactgcgcca cttcactgcc atcagcattg aaaatgcgat
     6661 agtgaaaatc cagttcagga tcatacggcg gctcaaccac cagcagctgg tcaaacccta
     6721 cccccaggtg ccgatccgcc agccgacgaa tcagctccgg tgaaaaaaag acattctgcg
     6781 ttaccgcgtc gacgaccata aaatcgttgc caaggccatg cattttcgag aactgcatca
     6841 tttactccaa tcacgcgggt acagaaactg acttttagta attcacctgg gatggaccat
     6901 cccagtggcg cgatcgtttt tatccggcac cgtggattgc gtttgcgtct ctaccggttt
     6961 ggtcggcggc ggtgcatttt tatctgcagg cgggaaatag agcggacctt tcagaccgca
     7021 gcccgtcagg ctgaagagag taagtaatac agtgagtacc ttaaacacgt ttttcattat
     7081 ggattgcctg taaagttcat cgctttctgc tttctatcat cgcaggagaa ggcgtaaaag
     7141 caagcgttta gcgactcttt atgaagtctg aaaagtgagg aaatgctgcg agtataacgc
     7201 ttactcgtga ggatttatgt tctgctggca gtttcgtgat cggcacctcg cgccaggatg
     7261 gctttatgca ctatcgcaat gtggtttgtt tgttggcttt tttagttccg tcagcgtgag
     7321 gatgctcgct cggtaaacct gatcataatg tttaccagaa acagggaaag gcgtggtgta
     7381 actgcgccct aatgaagaga caaatctttc gccacacaga gacatcatta ttccggtgac
     7441 ggatgaatca tgccggaacg cagcagcaga ggtgcaaact ttgaagccct gaccccatgc
     7501 cgctatactg ccgccatcac ttaaaaacag gatacaacaa tgaacgacag tgaatttcat
     7561 cgcctggctg atcaactgtg gctgaccatt gaagagcatc tggacgactg ggatggcgac
     7621 agcgatatcg actgcgaaat caacggcggc gtgctgacca ttacctttga aaacggcagc
     7681 aaaatcatta tcaaccgcca ggaaccgctg caccaggtat ggctggcaac caaacaaggc
     7741 ggctaccatt ttgatctgaa aggcgatgag tggatttgcg atcgcagcgg cgaaaccttc
     7801 tgggatttgc tggaacaggc ggcgacgcag caggcgggtg aaacagtcag tttccgctaa
     7861 gattgcatgc cggataagcc tcgctttccg gcacgttcat cacgaaaaat attgctgtaa
     7921 tagcggcgta tcgtgatcct gattggcagg cggcatgtta ccgatagatt ttgtgcggaa
     7981 cggaatcacc tgttcacgac catcaacctt cacaatctga tagaactgcg gcaggttgaa
     8041 gttgatgaag cttgagccgt aggtgaagcg gtcgtgcgat gacgagtaga agcgactgac
     8101 gtcacgcacc agttcctctt tgctgccttc gcagtggtga tacacctcaa cccggttgct
     8161 ttcgtcaaga atgtagatat taaagccatt ctcgtcttgc gtttcttcga agaagaactg
     8221 gatgatccct tcactagcaa agccgtccac caccgccggt aatttgacgt gattggtttc
     8281 aacctgcact gacaggccgt gcagtttgtt atgcgaaata gcgccataaa actcgatggc
     8341 gttctccagc ttctgtaccg atacattcag gcgttcgaag aacaagcccc aggtttgacc
     8401 agaaacgcgc agcgccttga aacgcccggt ttcctggcgg gtgctggaaa ggcgcagttc
     8461 aatacactca gaaaccagtt gctgcacgcg agtacggatt aacccgcaca gatgctggct
     8521 ataacagaag acttccacgc tatctggcgg tgcggcgtcc tgatgcattt tgccgagaat
     8581 agttttcagg gcttcgatca tcgattgctc gccgttgaag tgcagcgtac gcacttcgtt
     8641 ccacgagttg cggtacagca ggtcaacgct acctaccagg caattttgat tctcgccaaa
     8701 gctgaagaca tccagcttac ggaaatcaaa atgcaccacc tgattgcgga acgccgctgt
     8761 cgggtcatat tccaggttaa cgataatcgc cagatggcgg atctcacacg ggctgtagag
     8821 cgccttcggt gtcggtgcag gtaagcgcag cgggaaatgg tgcgacacgt cggcgaccat
     8881 ctcctgcaac ttaggcaaat cgacaatgcc gttaccttta atatacaaac gggtgcgcga
     8941 ggtcagcagg ccgttaaacc atgcccacgc caccagtttg ttcaggtaac ggttatattc
     9001 cagcggctga tggctgatga tcgactcaat atttggcgcg cggttataca gataccaacc
     9061 tgaacggtta gcacggcccg gcggcacata aataaaggtc agattcggtt ccgagagatc
     9121 gggtgaaatc tgcgggttta ccagcgtcac tttacctggt aatgcttcaa acgcggcata
     9181 cagcttacgg gtcagcacgc cgatatcctg cggactggcg gagacgctaa ggttattgcg
     9241 acgcgcaaag cggatcagat tacggtagct ctgcatcatc gcgtcgagca actcgttgtg
     9301 cgcctcacgc acctgatcaa tcttccagtt agcgcggtta tcgagcattg ccagacgagc
     9361 ttcgtcccaa ccccactcgc tcactaactg gctcaacact gcgcgacgcc agcctacgca
     9421 ggcgcgttca cggctgagct tttcgcacac ttttaaatag aagcagcgac gtactaaatc
     9481 cagacgggta aaatcttcaa tcgccgtcag gtattcagta acacgctcca gcatcatgca
     9541 gtatggatcg agaccaaacg atacaatctc accgtcgtgc aaacgctgtt tgatatcttt
     9601 cgccagcaga cgtgggttcg ggtattccca ggaataggct tccagcagca gtgttttcag
     9661 taccgctttg tatggggaat cgatactctt gtagagctgc caaaggctgg caccaaagta
     9721 ctcttcagca gaaagcgagc ttaagccacc gagatccagc cattcatttg gcgtcagcac
     9781 gccctgcgcg taaagcgtca tcacatagtc gtcgtaatgc tcttcttcgt cgcacggcac
     9841 catattccac agaatacgct taccggcgag acgcacggcg gtacgataaa attcgtcaag
     9901 cagcagtata tgctgggtgg agccacaatc ttcgcccccc aagctgccgc tttcattatg
     9961 acggaagcgg ttttcatcaa tcaggaagaa gctgacttcc acacccagcg aggcggccca
    10021 gctttccagc agactacatt tacgttgtag taactggcgc tcttcgctat cgagccagga
    10081 ttgatgacag acccagatat ccaggtcaga ggaacaactt tgccctacgg acgaggtgct
    10141 gcccatggtg tatacaccag taattggaag ctcacctttc ggtggatcct gtactgacat
    10201 tccacgatac agttcaagct cgttcaggta gtggcgttga gtttcatcag gcgtgtaaag
    10261 gcaaatgcct ttgggaacgt taccatcaag gtaacccggc attagcggat ggtgatagtg
    10321 caacaatgtc ggcagtagac tgtagacctg ttggaatgca ggccccatag cagcaagcgc
    10381 gcgatccaca cgcaattgat ttatggcatc cagtctctgt ttcagagtct caatatagag
    10441 gtacaagacg tatcgcctga tttgctaccc gtcatgactg tgattccgcc aacatcaacg
    10501 gtaacacgcg gcattcggga tatttcgtat gtcaaaggta accgttacca cttttcgcgc
    10561 ctggtttttt tagtttcacg acgaaaaaat ggtctaaaac gtgatcaatt taacaccttg
    10621 ctgattgacc gtaaagaaag atgcgctaca tacaagtgta gcaccgttta ttctctgtaa
    10681 attccttatt acgacggcgt gaaacgcctg tcaggatcca ctgccagacc tcattttacg
    10741 gtttgcgcag gcgtctacgt ttcaccacaa cactgacatc actctggcaa ggatgttagg
    10801 atggaccacg gatgataatg acggtaacaa gcatgttaga caatgtttta agaattgcca
    10861 cacgccaaag cccacttgca ctctggcagg cacactatgt caaagacaag ttgatggcga
    10921 gccatccggg cctggtcgtt gaactggtac cgatggtgac gcgcggcgat gtgattcttg
    10981 atacgccgct ggcgaaagta ggcggaaaag gcttatttgt aaaagagctg gaagtcgcgc
    11041 tcctcgaaaa tcgcgccgat atcgccgtac attcaatgaa agatgtgccg gttgaattcc
    11101 cgcaaggtct gggactggtc actatttgtg agcgtgaaga tcctcgcgat gcctttgtgt
    11161 ccaataacta tgacagtctg gatgcgttac cagcaggcag tatcgtcggg acgtccagtt
    11221 tacgtcgcca gtgccaactg gctgaacgcc gcccggatct gattatccgc tccctgcgcg
    11281 gcaacgtcgg cactcgcctg agcaaactag ataacggcga atacgatgcc atcattcttg
    11341 ccgtagccgg actaaaacgt ttaggtctgg agtcccgcat tcgcgccgcg ttaccacccg
    11401 agatttctct tccagcggta ggacaaggtg cggtcggtat tgaatgccgc cttgatgatg
    11461 cacgcactcg cgagctgctt gccgcgctga atcaccacga aactgcactg cgcgttaccg
    11521 cagaacgcgc catgaatacc cgtctcgaag gcggatgtca ggtgccaatt ggtagctacg
    11581 ccgagcttat tgatggtgaa atctggctgc gtgcgctggt cggcgcgccg gacggttcgc
    11641 agattattcg cggtgaacgc cgcggtgcgc cgcaagatgc cgaacaaatg gggatttcgc
    11701 tggcagaaga gctactgaat aacggcgcgc gcgagatcct cgctgaagtc tataacggag
    11761 acgccccggc atgagtatcc tggtcacccg cccgtctccc gctggagaag agttagtgag
    11821 ccgtctgcgc acactggggc aggtggcctg gcattttccg ctgattgagt tttctccggg
    11881 tcgacaatta ccgcaacttg ctgatcaact ggcggcgctg ggggagagcg atctgttgtt
    11941 tgccctctcg caacacgcgg ttgcttttgc ccaatcacag ctgcatcagc aagatcgtaa
    12001 atggccccga ctacctgatt atttcgccat tggacgcacc accgcactgg cgctacatac
    12061 cgtaagcgga cagaagattc tctacccgca ggatcgggaa atcagcgaag tcttgctaca
    12121 attacctgaa ttacaaaata ttgcgggcaa acgtgcgctg atattacgtg gcaatggcgg
    12181 tcgtgagcta attggggata ccctgacagc gcgcggtgct gaggtcactt tttgtgaatg
    12241 ttatcaacga tgcgcaatcc attacgatgg tgcagaagaa gcgatgcgct ggcaatcccg
    12301 cgaggtgacg atggtcgttg ttaccagcgg tgaaatgttg cagcaactct ggtcgctgat
    12361 cccacaatgg tatcgtgagc actggttact acgctgtcga ctattggtcg tcagtgagcg
    12421 tttggcgaaa ctcgcccggg aactgggctg gcaagacatt aaggtcgccg ataacgctga
    12481 caacgatgcg cttttacggg cattacaata actctcataa caggaagcca taatgacgga
    12541 acaagaaaaa acctccgccg tggttgaaga gaccagggag gccgtggaca ccacgtcaca
    12601 acctgtcgca acagaaaaaa agagtaagaa caataccgca ttgattctca gcgcggtggc
    12661 tatcgctatt gctctggcgg cgggcgtcgg tttgtatggc tggggtaaac aacaggccgt
    12721 caatcagacc gccaccagcg atgccctggc taaccaactg acggcattgc aaaaagccca
    12781 ggagagccaa aaagccgagc tggaaggcat tattaagcaa caagctgcac agcttaagca
    12841 ggcgaatcgt cagcaagaaa cgctggcaaa acagttggat gaagtccaac aaaaggtcgc
    12901 caccatttcc ggcagcgatg ctaaaacctg gctgctggct caggccgatt ttctggtgaa
    12961 actcgccgga cggaagctgt ggagcgatca ggacgtcacg accgctgcag cgttgctgaa
    13021 aagtgcagat gccagcctgg cggatatgaa tgacccgagt ctgattaccg ttcgtcgggc
    13081 aattaccgat gatatcgcca gcctttctgc agtttcgcag gtggattatg acggcatcat
    13141 ccttaagctt aatcagcttt caaatcaggt agataacctg cgtctggccg ataatgacag
    13201 cgatggttcg ccgatggatt ccgatggtga agagctttcc agttccatca gcgaatggcg
    13261 tatcaatctg caaaaaagct ggcagaactt tatggacaac ttcattacga ttcgccgtcg
    13321 tgatgacacc gccgtaccgc tgttagcgcc aaatcaggat atctatctgc gcgaaaatat
    13381 tcgctctcgc ctgctggtcg cagcacaagc tgtaccgcgt caccaggaag agacttatcg
    13441 ccaggcgctg gagaacgtct ccacctgggt acgtgcttac tacgatactg atgatgccac
    13501 caccaaagcg ttcctcgacg aggtggacca gttaagccag caaaatatct cgatggatct
    13561 tccggaaacc ctgcaaagcc aggcgatgct ggaaaaactg atgcagactc gcgtgcgtaa
    13621 cctgctggca caaccggcag cagggacaac ggaagctaaa cctgcacctg cacctgcacc
    13681 tgcacctgca cctgcaccgc aaggtgatac tccggcagcc gcgccgcaag gagaataacc
    13741 atgctaaaag tgttattact ctttgttttg ctgattgcgg ggatagtggt tggcccgatg
    13801 attgccggtc atcagggtta tgtgctgatc cagaccgaca actacaatat cgaaaccagc
    13861 gtcacgggcc tggcgatcat attgattctg gcgatggtag tgctgtttgc cattgagtgg
    13921 ctactgcggc ggatcttccg cactggcgcg cacacccgtg ggtggtttgt cgggcgtaag
    13981 cgtcgccgtg cacgtaagca gaccgaacag gcgctgctga aactggcgga aggcgattat
    14041 cagcaagttg aaaagctgat ggcgaaaaat gccgatcacg ctgaacaacc ggtggtgaac
    14101 tatctactgg ctgccgaagc cgcgcaacaa cgtggtgatg aagcgcgcgc caaccaacat
    14161 ctggaacgcg cagcggagct ggccggcaat gacaccattc cggtagaaat cacccgtgta
    14221 cgtctgcaac tggcccgtaa tgaaaaccat gctgcacgcc acggcgtgga taagctgctg
    14281 gaagttacgc cacgccatcc ggaagtatta cgtctggcgg aacaggcgta tatccgcaca
    14341 ggtgcatgga gttctctgct ggatatcatc ccatcaatgg cgaaagccca tgttggtgat
    14401 gaagaacatc gtgcaatgct ggaacaacag gcatggattg gcctgatgga tcaggcgcgt
    14461 gccgataacg gtagtgaagg tttgcgtaac tggtggaaaa accaaagccg gaaaacgcgt
    14521 catcaagtag cgttgcaggt ggcaatggcg gaacatctta ttgaatgtga cgatcatgat
    14581 actgcccagc aaattatcat cgatggcctg aaacgccagt acgacgatcg cctactgctg
    14641 ccgattcctc gactgaaaac caacaatccg gaacagctgg aaaaagtgct gcgccagcag
    14701 atcaaaaacg tcggcgatcg cccgctgttg tggagcacac tgggccagtc actgatgaag
    14761 cacggagaat ggcaggaagc atcgctcgcc ttccgcgcag cgctgaaaca acgtccggac
    14821 gcctacgatt acgcatggct tgccgacgcg ctggacagac tgcacaagcc ggaagaagct
    14881 gcagcgatgc gtcgcgacgg tttgatgtta acgttgcaga acaacccacc acagtagttc
    14941 cttctcaccc ggaggcaagc acctccggga cctttctgat gcataaaaaa acgcctgctc
    15001 ttattacgga gcaggcgtta aaacaggtct gtatgacaac aagtgggtgc ttcactcaac
    15061 gttgtgtcca tggtgtctga tgaggcataa gcgacatctg tcagtggacg ataagcaccg
    15121 taaacggctc tgcgtcattc cggagtttat gaggcactaa ggcgaacata agagatggaa
    15181 tgagcatcta ctcgtttatt atgccacaga gaatcgggaa ataacatccc ttaacacttg
    15241 ttatgagata attctgtaat cctctttgct tcctgagtaa taacttcctg agtgaatatt
    15301 taacctgagc ttgatcctac acatacttat tatgaatgat aaaattcatt caattaataa
    15361 cacatatatt aattgccgtt aaaactaaaa aaagcatcaa taatcaacgc gatataataa
    15421 acctgcctta catatcaact gcgccagagg taggattgaa aacgctctcc tgattttcca
    15481 attcattttc tggataaata aataatttat ttttgtcact attatttatg taatcatcct
    15541 gtcagggaga gggatctcaa ttatcaatgc ttaattacgt catcattttg atgacatgaa
    15601 accggagcat tttgcatgga attttcgttt tcacccaaac ttcttgttgt tgctgtcgcc
    15661 gccgctcttc ctctcatggc cagcgcagca gataccccgt caactgccac cgcacgcaaa
    15721 ggctttgccg gatacgatca cccaaaccag tatccacaga acgctgcaat ttattgaatt
    15781 tgcacgatca tgtaggccgg ataaggcgtt tacgccgcat ccggcaatca accgcaggcg
    15841 gccacaaaat ggccgctatt ttacttattc accaccagca aatgtgcctg cataatgtca
    15901 ctggccgggc gaccgtgcgc cagcaaatca gccattgctt taagatatgg cggtagatgg
    15961 cggaaataac gctgataccc ggcacacaaa taattcagcc ccggtttgcc gctggcatcg
    16021 agcatgaagc ggtgtttcgg gcagcctccc cagcacgctt ttaacacgtt acaactgcga
    16081 cactgcgccg gtaattgctt aaatttatct tcaccaaaca cctgctgttg cggggaatcg
    16141 atcatttctg caattgtttg ctgatgcata ttccccagcc gatattgcgg ataaacatag
    16201 tgatcgcagg cgtaaacgtc gccgttgtgc tcaacaatca ccgagcgccc acaggttggc
    16261 tgatgatggc aaaccgcacc cggcgcaccg acaaaattgg caaacgccca ttcgatattc
    16321 atcacgaaaa tcttgccgac gtcgcgtttg atccagtggt caaaaatcgc caccagaaat
    16381 tcaccaaatt catcggggcg cacagaccat tccgtcagtt ccccctgaat atcaccaggc
    16441 gcatgtagtt tcagtccatc gctggcagct gtttcatcag ctaggcgctc gaccaccgga
    16501 ataaactgga tgaattcgac tcccgcatcg cacaaaaaat cataaacctg caacggttgc
    16561 tgcgcgctgg tgcggttgac gcagaccagc acgttatagt cgacatgatg tttttgcagc
    16621 agcgtcaggg cacgcatcac cagcttatgc gttggtctgc cacctttggt cacgcgatat
    16681 tgattgtgga tctcagccgg accgtccagc gataacccaa caagaaaatg attttctgcc
    16741 agaaatgcac accatttatc atcgagcagc acgccgttag tctggaagct gttacttatc
    16801 ttcctgccag caccgtattt cgcctgtaac tttacggcac ggcggtaaaa atccagcccg
    16861 agtaacgttg gttcgccgcc ctgccaggta aaagccactt cgttttgcgg ttcgctggca
    16921 gcgatatagt gacggacata cgcttccagc gtgtcatcgt ccatatgcgt gactggcttt
    16981 tcgcggtaaa gggattgttt ttcgagataa aaacagtagt cacagttcag attacaatcg
    17041 gaaccactcg gtttcgccat cacatgaaaa gcacgcgttg gaacctgttg cagcatggtc
    17101 actccttgcc tgaagtcatc cttcaatacg ttaagggcga aatcataccg tggggcaatg
    17161 agttattacg ggcgtttatt gcgaagagag ggtgacgaaa tgcacagaaa acaaaaaacc
    17221 ccgccgaagc ggggttcaaa attggtcggc gagagaggat tcgaacctcc gacccactgg
    17281 tcccaaacca gttgcgctac caagctgcgc tactcgccga aatactgctt tttgaatttt
    17341 tagttcaatt atttaaagtc gtggtgcgag gggggggact tgaaccccca cgtccgtaag
    17401 gacactaaca cctgaagcta gcgcgtctac caattccgcc accttcgcat tgtcacaact
    17461 tctaataatg gggtggctaa tgggattcga acccacgaca actggaatca caatccaggg
    17521 ctctaccaac tgagctatag ccaccactac aaatcttgtt acgcggtatt actaccaccg
    17581 cagctcaagc gccgggacta aatggcgcgc ccgacaggat tcgaacctga gacctctgcc
    17641 tccggagggc agcgctctat ccagctgagc tacgggcgct tagcgccgtt gcgggggtgg
    17701 ataatacgga cttcccaccc ctctgtctag tcccttttta aataaattgc gcgtttggtt
    17761 acggtttgtg cgttttgctg cttattcctc cagtttatgc gctttcccgt ggcgattaag
    17821 gccaaaaact ttataaatcg ccgtcaccgc cagcataaag atgatgccaa caaacagcga
    17881 catacgcgta tcttcattaa agtacatgcc gatcaaaacg caaatcagga atgccattgt
    17941 tacgtagttg gcccacggga acaggattga gcggaacgga tggctggcaa tcgccgcttt
    18001 atgtgcacgc cgaaaacgca gctggcttat caatatcaca aaccatggca ccatccccgg
    18061 aagcacgctg gcactgtaga cgtagacaaa cacacgctgc ggattgggaa tgatgtagtt
    18121 caggcatgag ccaattagca gaatagcaat agatactgcc acacccgcaa ctggtacgcc
    18181 gtgacgggaa actttcgcca tcgccgccgg taactgacgg tttttcgcca gtgcgtagag
    18241 catacgtccg caactgtaca tgccgctgtt acagccagag agcgcagccg tcagcaccac
    18301 aaagttgata atgcccgccg ctgcggtaat accgattttg gcaaaagtca gtacgaacgg
    18361 gctgccgctg ctgccacttt cattccacgg gaagatggtg acgataacga aaatcgcgcc
    18421 tacgtagaaa atcaggatcc gccacagcac tttgcctacg gcactgcgca acgtcacctg
    18481 cggattcttc gcttcaccgg cagtaatgcc aatcagctcc acgccctggt aggacgccac
    18541 cacaatacac agcgcggtca ggaacccttt ccagccaccc gcaaagaaac cgccatgctc
    18601 tgtgagattg ctaaaaccaa tcgactgccc gccattgcca aagccaaaga aaatcacgcc
    18661 caggccaatg acaatcatca cgataatcgt ggtgactttg atcatcgcga accagaactc
    18721 gatttcgccg tacaaccgca ccgccgccag attcgccaac gccaccagcg ccactgcgat
    18781 caatgcgggt atccactgcg ccatctccgg gaaccagaac tggacataaa cgccaacggc
    18841 ggtgatttct gagatcccca ccgccatcca cataaaccaa taagaccagg cggtgagata
    18901 accaaagaac gggctcatat aacgatgcgc ataaacggcg aacgaaccgg taaccggttc
    18961 gaggaacaac atttcgccca ttgaacgcat gatgaaaaag acgaatagcc cggcgatgat
    19021 ataggccaac aatacggatg gcccggccca tttcaaggta ctggcggccc ccataaacag
    19081 gccgacgcca atggtgcccc ccagggcgat gagttcgata tgtcgagctt ccagcccacg
    19141 ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat gctttccggt
    19201 gttaccggtt atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa ttcaggagaa
    19261 atggcaaata aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca gacaggcgac
    19321 ggagtgacca ctccgtcgct ttacaaagag aggaaaatca gaggttgccg gtgtagtgcc
    19381 agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc gaaagcaggc
    19441 ggtagagcca ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt acgtgaccgg
    19501 tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc atctggatgc accagacggc
    19561 agtcgcgcat gaagatctcc tgctttggcg atcccatcgc aacggtgacg atttgcgctc
    19621 cgctggcatg aatgcgttca aacagcgcct gacgctgctc gggtttaaaa taaccatcct
    19681 gactgccaac gatattcaca ttccactggt tgcgcagttt agcttcagtt tgcgccagca
    19741 cttcaggttt accgcccaca agaaataccg gcgtcccttc tttgcctgcg cgcgccatca
    19801 gctcttccca gagatcggca ccggcaacgc gggaaacctg cgcctgcggg tactttttac
    19861 gtactgaacg tacaacgctg atgccatccg catatttaaa ttcggcagcg ttaattaact
    19921 ccctgacctc ggcgttatct tcaatggtca gcattttttc agcattaatg gcaaccagcg
    19981 ttccctgctt aagctgcccg tcagcaaaca gataatcgag ggcgtgctgc atatcacgcc
    20041 aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg ttattcattg
    20101 ttatccttca acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga gtccggcgct
    20161 ttcaaaaagc cagtacaaca gttttgcgat catcagacat gcgccgaaga ccacgataaa
    20221 aaagactacg cgtgagacaa acgaatccag cccttcacgt gccagcacga tcatattgaa
    20281 gatcgcccca aagcagaaac tgtgcaatat cgcagcctta tagcgattag gctcgcggtt
    20341 gcccagctca tacagccagt cgaaccattt gatgatcaga ccaaccacga tcgccccgag
    20401 cgggatgaac aacgcgccgc ccatcaccac cagtgagcct ataagcgtag gcgagatcgc
    20461 cagtccggag tggttattca gcacttccct ggtaaagtag ttggctgagt tcagcaccat
    20521 actcgggcga cccggccaca gccaggaagg gataaagaca tagaaatcgc ggacaattgg
    20581 agccaggccc tggaagtcga tgttgtcgta gttctgcaac agcaacgcca gattctccca
    20641 cggcgagaag gtgtcgcgag tgagatagag aaacgtatag aacgcttcat cgccgctcac
    20701 attcattcca tagcgtttta gcgccagcca gaacatgccc acaatcccca gcacgcccgc
    20761 cgccgccagc atccacaacg aaatccagcc gcgaataatg ccaataaaca ggaagatagc
    20821 gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc atataagtca gcaagccaaa
    20881 ggcgaccgtg ctgacgagga aaaacagcca cgctttgcta tcctggcgca gaaagtagac
    20941 caccagcatc gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc cggagacttc
    21001 actggagaag atctgactgt aggagttaag ccggaacagc aaaaagccgt tgtgcatgaa
    21061 gaagatgccg acgcttacca gcgcgatgcc catcaggatc acccacgtaa gattggtctc
    21121 cacgcggttc atggtaaaca gcggacggcg cggtacatca gcaacgcgtt tgcgtaggcg
    21181 ggttttgtag gtgacatagt aaaccgcgta gaagcagccc gcagaaagca acgcctgcaa
    21241 caagatttct ggcggcgcga caccaacatc aaagcgaaat accagcacgc tggtcagcgg
    21301 gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag aagacattga agttaaagcg
    21361 cacacggcga aactcaaacc aagtcagcgt ggcaataaac agcgtgcaga gcagccagac
    21421 cacaaacagg ccactgaatt gcagcagact catgcgacct ccccggcggc aatcgccaac
    21481 acccgctgcc agccttgtag atagttaggg ctaaagaagg caatggtgtt tttatccacc
    21541 gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc ggtagtaaac
    21601 agcaccggca aatgctgttc cgtcatatcc tgccagaacg gattttcccg gttaagcaca
    21661 caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa tgccctgctg gcgggcaaaa
    21721 ataaagtaac caagatcgca ctgacgaagt agcgccagat aggcgtcaaa ttccagtttt
    21781 tcgctcaaaa tttgcagatt ttcttcgctg aataactcca gccccgcctg acgaacttcc
    21841 tcaatgtacg cttcgttatt aggcggatat cccatcggca ccaccacttt taccgtatcg
    21901 ccaaattgct gatgaacggc gcgcaaggca gcaatatggt cattgctgcg gtcaccggag
    21961 ttccccacca gaatggtcat tttcccttca cgttgccgat cgttcgccat cgtattgaga
    22021 gaagggtcca tccgcgtcgg gaagtacagc agttcgcccc gcacctttgg atgcgttttg
    22081 gcaaaaaagc tcaaatcacc gcgggtggca aatacacagc cgactcgctt ttgcgccagg
    22141 cgacgtagcg ggtaaaaaag cttatatctc aagccactgg aaagctcata caggtctgcc
    22201 ccccagatat gccagaaaaa ctggctgggc ttaatgccac cactcagcag agccagccac
    22261 agtgtgggat tgaactgacc gtggaaaaaa aatcgctgct gacggttagc ttttgctttc
    22321 gcgatgaccg cttccgccag cgattttttc ccagggaaaa attgcacaga aagcgccgga
    22381 cagctatcac ttaagccgtc gtccttgcca acaaccataa actcgcgcgc gtgctcgctc
    22441 gtcgcggcca gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg agggatatcc
    22501 gatcccagta cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa aacacgccac
    22561 aacaaagaga aaaatagacg atataagttg ccatatatgc ctgcgccgcg cccagtgcac
    22621 catgcgcagg gattagccag tgggcaaata ccatcaataa agtgaactgg ctgacttccg
    22681 ccagaatata aaaccgcagt gacgctttgg cgatcaccag ataaccaaag acataagcgc
    22741 ccacttttag cacatcaccc actaactgcc aggcaaagag atcgcgcata gcggtaaatt
    22801 tattcgacaa cagcaaccag atagcaaaat cacgcagcaa ccagacggta aaactcgccg
    22861 ccgccactgc cggtaagacg aattttagcg atttaaccac ttcccgggtg atatcgcgct
    22921 tttccgttag ccgcgacaac gtgggcagca aatatacgct gaacgatgcc gtaatgaatt
    22981 gcaggtaggc atcggaaata ctgctcaccc cttgccagat ccccacttca tcccagctat
    23041 actgcgccgc cagcagttta cgcatcatga tgtaagcaac aggcaaggtc accgacgtaa
    23101 tcaacgccat gagcgtaaat ttgctcaact gccctgccag accgttatcc cagctgggtt
    23161 tcagatagct taacgggatg acaccacgtt tgatcaacat gatggcggca ggaattacca
    23221 ccagcgcggg aatcagcgcc agacccagca acgccccttc ataaccgccc aaacggtaac
    23281 tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat cagcgataac gcattacctg
    23341 cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt gccccaggcg atccccattt
    23401 gcaccagcgc cactaaacgc accagcccct gatagtcggt attaccaaac aatccctggc
    23461 tgattggcgc agctgccagc acaaaaacca gcgccatcag cgtagagaaa ccaagtacca
    23521 tcgctgatga agtgccgacc acgcggcgca gctgttgcgg attatcatgg tactgggcaa
    23581 cgtatttggt tacaccgtta aagatgccag ccccggcaag cacgccgagc acggtaatca
    23641 actggcggaa atttgccgcc agcccaagcc ccgccggacc aaatgacacc gccagcaact
    23701 taccgaccag taacccggca ccaatcttga ccagtgtact ggccgccgtc cacaaggacg
    23761 cttttgccaa cgacatatca ggagaagtag ttcaacaaag tcgcaattac cgtacgctga
    23821 ttgacgggcg acaggttgta gaacagcggc aggcgcagca ggcgctcgct ctctttggtg
    23881 gtgtagcgat cttcaccgtg gaactcacca aagtgttccc ccgcagggca accgtgcagc
    23941 ggaatgtaat gaaacaccgc catgatttcc gcttctttca gaaagttaat caacgcgctc
    24001 cggtcatcaa tatcccgcag tttaatgtag aacatatgcg cgttctgcac gcagccatcg
    24061 ggaatcgacg gcagctcgat acgcccggct ttcgccagag gcgctaacgc atcgtagtaa
    24121 ttttgccaca gcgccagacg ttgctggttg atacgatccg ctgcttccag ttgcgcccac
    24181 aggtatgcag cttgcagatc ggacatcaaa tagctggagc caatatcgcg ccaggtatat
    24241 ttatcgacct gaccacggaa gaactggctg cggttagtgc ccttttcacg gatgatctcg
    24301 gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc acccgccgtg
    24361 tagtttttgg tttcatggaa gctaaagcag ccaatatgac caatggttcc cagtgcacgc
    24421 cctttgtaag tggacatcac gccctgagcg gcatcttcca ccacaaacaa attatgcttt
    24481 ttcgccaacg ccataatggt gtccatttcg caggccacac ccgcgtaatg gaccggcacg
    24541 ataacgcgcg ttttgtcggt gatcgctgct tcaatcaacg tttcgtcgat gttcatggtg
    24601 tccgggcgaa catccacaaa gacgattttc gcgccacgca gcacaaaggc attggcggtg
    24661 gagacaaagg tgtagctcgg catgatcact tcatcgccag gctggatatc gagcagcagc
    24721 gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta acactttggc gctgccaaaa
    24781 cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc catcgccaca cagtttgccg
    24841 ctacccattg ccgactgcat atagtcgagt tcggttccca ccaccggcgg tgcgttaaat
    24901 ggaatcatgt gatcacctgt ataaccagta cgcggtgctt tctacattcg caccactttg
    24961 tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc gccacccgca aagttgtttt
    25021 accgcgagca tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg caccgcgtcc
    25081 agccagcagg ccaattcgcg catctgtcgc attgagttcc cgtaaagaga catagccgcg
    25141 aatatcgccg gacgccgcac gtagaatcag acattgatga tcaaaggtgc cgcgcacggc
    25201 attttcaatc cactgtgcat aaaagcgacc gctggcgtca ggcgcatacc acggcgcacg
    25261 aaaacggctt tgcgcaaatg cggcgctagc taactgacgt aatgcgggaa tatcggtctc
    25321 ttgtgccacg acagcaccgc tatcactgac attgttcacg ggtagcgcca aatcaacttc
    25381 accttctacc agggagaatc ccagctgttg cagggcatcc agttcacccg tatttgatgc
    25441 cgcaattttg gcctgcaccc gtgaccacgg cgctaacgcg tctggcgtca ggagcggtgc
    25501 ttcagacgta atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt cccaggttag
    25561 tggctcaata ctggcgcgga cgggcacgaa gtaactccag cagatattgg ccgtagccag
    25621 ttttcgctaa tgaactggca gcacgcttca caccctcgtc atcgagccag ccgttacgcc
    25681 aggcgatctc ttccaggcag gcaatcttaa agccctggcg tttttctact gtctgcacaa
    25741 aggtactggc ttcaatcagg ctgtcgtgag tgccagtgtc cagccaggca aatccgcgcc
    25801 cgagcagttc aacggtcagg ttgcccgcct cgaggtacat ctggttgatg gaggtaatct
    25861 ccagttcacc acgctccgac ggcttcacct gctttgcgta ctccacgact ttactgtcgt
    25921 agaaataaag cccggtcacc gcccagtttg acttcggctg ttttggcttt tcttccagcg
    25981 agatagcgcg gaaattgtcg tcaaactcca ccacgccaaa gcgttcctgg tccatcacct
    26041 gatagccaaa taccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc ttcggactga
    26101 agccctgacc gaagaagatg ttatcgccca gcaccagaca agaaggttca ccgttgagga
    26161 aggtttcacc gatgataaag gcctgcacca gaccgtccgg gctgggctgt tcggcgtatt
    26221 ccagctggat accgaactca ctaccatcgc ccagcaggcg ctggaaataa cctttatctt
    26281 ccggcgtagt gatgatgaga atttcgcgga taccggccaa catcagcacc gacagcggat
    26341 agtaaatcat tggcttatcg taaatcggca acagttgctt cgatacgccg cgcgtaatcg
    26401 gatgcaatcg ggtgccggaa ccgcccgcca ggataatacc tttcatgttt gcctccgctg
    26461 aaaattagcc tttcagacct aaacgctcgc cctgatagct gccgtcctgc acctgcttcc
    26521 accagctttc attagccaga taccactgca ccgttttacg cattccactt tcaaaggttt
    26581 cctgtggcag ccagccaagt tcacgggcaa ttttcgaagc atcaatggca taacgcagat
    26641 catgccccgg acggtcagcg acaaaggtga tcaagtcacg atagtgcacc acgccgtgcg
    26701 gcttgttcgg agccagttct tccagcagct cgcaaatggt ttccacaaca tcgagattct
    26761 tacgctcgtt gtgaccacca atattatagg tttcaccgac tttcccggtg gtcgccacgc
    26821 aatacagcgc gcgggcgtga tcttccacat acagccagtc acggatttgc tgcccgttgc
    26881 catataccgg cagcggttta cccgccagcg cgttgaggat catcagcggg atcagttttt
    26941 ccggaaagtg gtaaggaccg tagttattcg agcagttggt gataagcgtc ggcagaccgt
    27001 aggtccgcag ccaggcgcgc accagatggt cgctgctggc ttttgacgcg gaataagggc
    27061 tgctcggcgc atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc aggtcaccat
    27121 atacttcgtc ggtggagata tgatgaaaac ggaacgctga ttttttatct tccgtcagcg
    27181 tattccagta agcccgcgcc gcttcaagca atgtataagt cccgacaatg ttggtttcaa
    27241 taaacgctgc cgggccgtca atagaacggt caacatggct ttcggctgcc agatgcatga
    27301 cacagtctgg ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg cagatatcaa
    27361 ctttctcaaa ggcaaagcga tcgctttgcg cgaccggtgc cagcgacatc aggtttccgg
    27421 cgtaggtcag cttatcgacc actaccaccg cgtcgctcgt ttcgttgatg atataacgca
    27481 ccagcgccga gccaataaac ccggcaccac ctgttatcag aatttttctc atcagcgcca
    27541 gactcctttg gcatcgacga catactgctg atggacattg tcgccattga taactttaaa
    27601 ctgactatga tcgaccagca tcaccagcac gtctgccgtt gccagcgcct cgtcaagctg
    27661 cgccagagta caaagcccgg tcagtttttt cggcaactgg tggatgttag gctcaacaac
    27721 cagagtttcg ccgctatgcc actgggcgat cagttcagcg atttccatcg ccgggctttc
    27781 gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag caggcgattt tcagttcact
    27841 ggcgcgttta tcggtagccg ccaggcaatc agccaccgcc gctttcacct gatcgataac
    27901 ccagaacggt ttgtgatcgt tcacttcgcg cgcggtacgg ataagccgcg cctgctgggg
    27961 gttctgtgcc acgataaacc acggatcaac agcaatgcag tgaccgccca cgccagggcc
    28021 aggctgaaga atattaacgc gagggtgacg attcgccagg cgaatcagtt cccagacgtt
    28081 aatcccctga tcggcacaaa tcagcgacaa ttcattagca aaagcgatat tcacatcgcg
    28141 gaagctgttt tcggtgagct tacacatttc cgccgtccgc gagttagtga cgacacactc
    28201 accttcgagg aaaattttgt acagttcgct ggcgcgggtc gaacaaaccg gcgtcatacc
    28261 accaatcacg cgatcgtttt taatcagctc gaccataacc tggcccggta acacgcgttc
    28321 cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga aagtgagatc
    28381 cggacgcatc tctgctaacc attctgccat cttctcggtt gaccccaccg gcgaggtgga
    28441 ttcaaggatc accagcgcgc cttttttcag cactggcgca atggagcgag cagccgattc
    28501 aacgtaggtc atatctggct catgatcgcc tttaaacggc gtgggtacag caatcagcca
    28561 ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta cggcagtttt
    28621 tactacgctc gccaaatcag gctcgacgat atggatttcg ccacgattga tggtatcaac
    28681 cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa acgctgctgc
    28741 tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac tcatagtgat
    28801 atccgattat tttttaacgc ttccagaatg cgagagcatg cctgaccatc accatacggg
    28861 ttatgggcgc ggctcatagt ttgatattcg ttttcgtctt ttaaaagacg cgtcacttcc
    28921 tcgacaattc gctgcttatc cgtgcctacc agacgcaccg tacccgccgt caccgcttcc
    28981 ggacgctcag tggtatcgcg catcaccagc accggtttcc ccagcgaagg cgcttcttcc
    29041 tgaatgccgc ctgagtcggt caaaatcagc caggcgtggt tcatcaacca gacaaacggt
    29101 aaatactcct ggggatcgat cagaatgaca tttttcacat gccccagaat gcgattgacc
    29161 ggctctctga cgttcgggtt gagatgcacc ggatagacaa tctggatgtc ctggtgcgtg
    29221 gtggcaatgt ctgccaacgc gtggcagatt tcttcaaaac cacgaccgaa actctcacgc
    29281 ctgtgaccgg tcaccagaat catcttttta ttggggtcga taaacgggta atttgccacc
    29341 agttctgaac gcagcgtgtc gctgctcatc acctgatcac gcacccataa cagtgcatca
    29401 atgactgtat taccagtaat gaagattcgg ctatccgcaa cgttttcacg cagcaagttt
    29461 tgccgggaag tttcggttgg agagaagtga tacatcgcca gatgcccggt caatgtacgg
    29521 ttagcctctt ccggccacgg cgaatagaga tcgcccgtgc gtaaaccagc ctcaacgtga
    29581 ccaacaggaa tacgctgata aaacgccgcc aggctggttg ccagcgtcgt tgttgtgtcg
    29641 ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa taggttttag cccttccaga
    29701 atccgacagg ttatctctgt caggccctgt cctggctgca ttatgttgag atcgtagtca
    29761 ggtacaatgg aaaagagttt cagcacctga tcgagcatct cccgatgctg cgcagtgacg
    29821 caaactttag cctcaaaaaa aggagctttt gccaacgcat gcaccaacgg agccatcttg
    29881 atggcttccg ggcgcgtacc aaatacagtc agtactttca catcgattct cttcgaataa
    29941 gcggcgagcg cctttgcgct cgccgcagca gtgttgctat ttcgagcaac ggcgggttaa
    30001 tgcgacacca gccccgatca gccccccgac aatgccccac ataatcatca ggaaggcacg
    30061 acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat aggtctgaaa
    30121 acgcggatcc agggttggac caacattcag ggtgtttaac atggcccgat tctgatcata
    30181 gtcgagatca aaggccggac cgacggcctg taaattttcc agtcgagcct ggagcattgg
    30241 acgcccaagc aggaacattt ctgaatcagg taattcctcg gcaggcacat ctgtcgcact
    30301 gcgcgaaata ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt tcatccggcg
    30361 gtcgtagatg gctttcgcca cctcttcctg acgcttcacc tgagctttca tctggatggt
    30421 gcgtgccgcc catgcgcctt tcagctcatc attcagatgg ctggctgcac gctggctggc
    30481 aaaagcaaca tactgacgta acaggttatt agcgtcaggc gcggtttcgg caataagctt
    30541 cacgctgtca ttgaccgcgc gggtaaagtc tccggggata aactggatgt tgttaatcat
    30601 ttcatccagc aacgccgcat cggctttgct gttgcccacc atccgctgtt tgtaatagtc
    30661 ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc agttgcataa caaactcttt
    30721 ataggcttcg tccatgaccg atggttgatc ggcagaagcc atgtttgaac ggacatccag
    30781 gttacgcaaa aattgctgct gcgagtaata tccccccagc atattcaccg ttggacgatc
    30841 ggtaatcgcc gtcgagctcc actcctgacg agcaaaaaaa gtatacgcca gcgcgattaa
    30901 cgcaaacgcc agccccatgc caataatcca tagcttccca gcccacaagg tacgaaacaa
    30961 cccacgaata tccagttcat tttcagcgtc ttcggccggt ttcccaggca ttggttgtgt
    31021 catcacatcc tcatttattt ggttaaattg gggctgccac cacgatttct acgcagtctg
    31081 cgttttacgc gcttaataaa gcgagcaact ttccaggcac gcttaatgca atatccatag
    31141 aggaagaatg ctagcaaaaa gagcaccagc atgacccact ccgggacaaa atgagaatat
    31201 tctgccagca cgccaatgga agcgagcagt gctgcggcaa gggtaatcag cacaaacgcc
    31261 tgacgggaag taaacccggc acgcatgatc aaatggtgaa tatgctgacg gtcaggagag
    31321 aatgggctca tgcctttacg caggcgacgg tacataatcg ccaccatatc cattagcgga
    31381 atggcgatta tccacaaagc ggtaaccggg ctgatgggat gggttttgcc ctgggtcgtt
    31441 tcgagcagga tccagataac ggtaaaacca atcagcgtac tgcccgcatc acccataaag
    31501 actttgtagc ggcgacccag gataccaagg ttaagcatga tgtatggcag gatggcggcg
    31561 atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa aatcataccg
    31621 attgctgcaa acgagacgca ggacaacccg cccagcaagc catcaatgcc atcaaccatg
    31681 ttgaacgcat taatggccgc ccagacggca aatagcgtca ggaagtaacc aaacggtccg
    31741 agcaccatct cccaggagcc aaagatataa cccaggctac tgagataaag tttgccgaac
    31801 accatcataa caatgccaac agcggcctgt atggtggcac ggatttttac gctgatatca
    31861 aaacggtcat ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag atagagagat
    31921 gcatgcggaa tatagtaatc gacaattccg aacgtgaagc aaatccctgc gtaaaccgaa
    31981 atccccccaa cgagaggtat caatccctgg tgacgtttgc ggaagtttgg tttatccact
    32041 aaaccgactt tttttgccac cttacgggca aaaaacagaa acagtgtcgt gaataaaaaa
    32101 atactgatga gatcagtact cactgtcagt aaattcacaa tgcatgctct cagagaaaat
    32161 tattagcaga agtataacca cgaagacctt tattcagaag ggaaagtccg ttccgaaccc
    32221 accagtcctg tgcaaaaatt aaccatttga tatgcaaatt gcataacatg ccagcaaatt
    32281 ccaggaattt tcctaacatc gccagcgcgg cattaagatt acagatataa aagcaaaacg
    32341 ccacgtaaac acgtggcgtt tttggcataa gacaaattta tgagcgtttc atcatttcga
    32401 agaaatcgtc attggtcttg gtcatggcca gtttattaat gaggaattcc attgcatcga
    32461 tttcgcccat cgggtgaatg attttgcgca ggatccacat tttctgcagt tcttcctgag
    32521 tcgtgagcag ctcttcttta cgggtaccgg aacggttgta gtcgatagcc gggaagacgc
    32581 gtttttcagc gatcttacga gagaggtgca gttccatgtt gcctgtacct ttaaactctt
    32641 cgtaaataac ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg ataatggtca
    32701 ggctgccgcc ctcttccacg ttacgcgccg caccgaagaa gcgtttcgga cgatgaaggg
    32761 cattggcgtc cacaccaccg gtcaacactt tacctgacgc cggaacaaca gtgttgtaag
    32821 cgcgcgccag acgagtgatg gagtcgagca gaatgataac gtctttcttg tgctcaacca
    32881 ggcgtttggc cttctcgatc accatttccg caacctgaac gtggcgagat gcgggttcgt
    32941 caaaggtaga agcaacaact tcacctttta ccagacgctg catctcggtt acttcttccg
    33001 gacgttcgtc gatcagcaga accatcagca cacaatccgg gtggttgtaa gcaatgctct
    33061 gagcaatgtt ctgcagcagc atagttttac cggctttcgg cggtgccaca atcagaccac
    33121 gctgaccacg accgataggt gatgccagat ccagtacgcg agcggttaaa tcttccgtag
    33181 aaccgttacc acgttccata cgcagacgag agtttgcgtg cagcggggtt aagttctcaa
    33241 agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta actttcagca
    33301 gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt accagagatg gtatcaccag
    33361 tgcggaggtt gaaacggcgg atttggctag gggaaacgta gatgtcatca ggaccggcga
    33421 ggtaggagct gtctgcggaa cggaggaaac caaatccatc ctgcaatatc tccagtacgc
    33481 catcaccaaa gatatcttcg ccactctttg cgtgctgctt caggatggca aaaataatgt
    33541 cctgcttacg catacgagcc aggttttcca gccccatatt ttcgccgaga gtgatcagct
    33601 cagaaaccgg cgtattcttt aattcggtaa gattcatagt ggtgtgagtt cttaaacttg
    33661 gggtaaatct cgaacttaat gttgtgaatg gtatggcagg atcatccatg cctgtttacg
    33721 gtcatcgact catgtctttt cgctgcctgg tcacaggaga gtacgcagaa ctgaaacgac
    33781 aagacggatt gagtgacgag ccagaaatct gttcacttcg catttaagat aaaacgggaa
    33841 gcgttggaaa ttacaagatt caaacttaat aaggtatgtt taatacgaag tcaacactaa
    33901 gttagcatga ctcacgccgg gcgtccagtt tttagcgacg gggcacccga acatgaaatt
    33961 cccttacgcc aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac
    34021 tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc
    34081 atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc
    34141 ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca
    34201 ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact
    34261 gtcgtcagtc aggtgaataa ttttatcgct catatataac tccacaggaa taagcctggc
    34321 gtgttggttt cgttgttggt gtaacattaa ccaactaaag gttgacttta tttcaccgga
    34381 tacgctttcg taaagcaata gtaagctgat attctaccac actatgagca aaacacattt
    34441 aacagaacag aagttttccg acttcgccct gcatccgaag gttgtagaaa tccttgaaaa
    34501 aaaagggttt cataactgta cgcccattca ggcactggcc cttccgctga cgctggcggg
    34561 tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt ttcttacgtc
    34621 aacgtttcat tatcttctct ctcatcctgc gattgccgat cgcaaggtga atcaaccacg
    34681 tgccttaatt atggcaccga cgcgtgaact tgccgtgcag atccatgccg acgcagaacc
    34741 gctggcggaa gctaccggcc tgaagctggg tctggcttac ggtggtgatg gctacgacaa
    34801 acagctgaaa gtgctggaaa gcggcgttga cattctgatt ggcactacgg ggcgtttaat
    34861 tgactacgcc aagcagaacc acattaacct cggtgccatt caggtggtgg tactggacga
    34921 agccgatcgc atgtacgatc tgggctttat taaagatatc cgctggctgt tccgccgtat
    34981 gccgcctgca aaccagcgcc tcaacatgct gttctccgcc acgctttcgt accgggtacg
    35041 tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac cggaacagaa
    35101 aacgggccac cgtattaaag aagagctttt ctacccttct aacgaagaaa aaatgcgttt
    35161 gctgcaaacg ctgatcgaag aagagtggcc agacagagcg attattttcg ccaataccaa
    35221 acaccgttgt gaagagatct ggggccacct ggcggcagat ggtcatcgtg tcggtttgtt
    35281 gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta cccgtggcga
    35341 tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc cggcagtgac
    35401 gcacgtcttt aactacgatt tacccgatga ctgtgaagat tacgttcacc gtattggtcg
    35461 tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag agtatgcatt
    35521 gaatttgcct gctattgaga cctatattgg tcactcaatt ccggtaagca aatacaatcc
    35581 ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc acgcgcccac gtacaggcaa
    35641 tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa taaaatgtca
    35701 tactcaaaat cattcaaact gtatcaagat ggcaagagaa tgaatcccga tgagcatact
    35761 gtagtaagtg attcaggtga aagaatgcag ccaacacaga gacagattga aggatgaaga
    35821 gtatgggttc cacctcgtcg ctgtatgcag ccattgatct cggttcgaat agttttcata
    35881 tgctggttgt gcgcgaggtg gctggaagca tccagacgct gacgcgaata aaacgcaaag
    35941 tgcgtctggc tgctggcctg aatagcgaaa atgccctgtc taatgaagca atggagcgcg
    36001 gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga tatccctccc tcgcaaattc
    36061 gcgttgtcgc tacggcgaca ttacgtcttg ccgtcaatgc gggtgatttt attgccaaag
    36121 cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa gcacgtctga
    36181 tttatcaggg cgttgctcac acaactggcg gtgccgatca gcgcctggtg gtggatatag
    36241 gcggtgccag tactgaactg gtaaccggca cgggtgcaca aaccacctcg ttgttcagcc
    36301 tgtcgatggg ctgcgtcacc tggctggaac gctattttgc cgatcgtaat ctggggcagg
    36361 aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt gccgatgaat
    36421 tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag gcgttacagg
    36481 aaatcatgat ggcacagggg atggatgaac gcattaccct ggaaaagttg cagcaattga
    36541 aacagcgagc cattcattgc ggtcggctgg aggaactgga gattgacggg ctgacgctgg
    36601 aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat cgccattttt accgaactga
    36661 atattcagtg tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc tacggcatgt
    36721 tgcatctggc cgtcgagcag gatattcgca gccgtacgct gcgtaatatt cagcgccgct
    36781 ttatgatcga tattgatcag gcgcagcgcg tagccaaagt tgcggctaac ttcttcgatc
    36841 aggtggaaaa ggaatggcat cttgaagcaa taagccgcga tttgctcatc agcgcctgtc
    36901 agcttcatga aatcggcctg agcgttgact ttaaacaagc gccgcaacac gctgcttatc
    36961 tggtgcgtaa tctggatctt cccggtttta cccccgcaca gaaaaaattg ctggcgacgc
    37021 tactgctcaa ccagactaat ccggtcgatc tctcatcgct gcatcagcaa aatgccgtac
    37081 cgccgcgcgt cgcagaacaa ctctgccgtt tactccgcct ggccatcatt tttgccagcc
    37141 gtcgccgtga cgatctcgtg ccagagatga cattacaggc taaccatgaa ctgttgacct
    37201 tgacgcttcc gcaaggttgg ctaacccaac atccgctggg taaagagatt attgctcagg
    37261 aaagccagtg gcagagctat gtccactggc cgctggaagt gcattaatga gtaagtgccg
    37321 gatgcgatgc tgacgcatct tttccggcct tgattatttc cctcgttttg ccgccatcat
    37381 cgctttcaga ttcgccagat ggctttgccc tttctgcatc cgttcttcgg cgctgaccac
    37441 tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa agcggctcgg
    37501 ctccgggcgc accagctcgc cgtactgacg gcgttcttta cacagcgtaa aggtcaattc
    37561 cttctgggcg cgggtaatgc cgacataggc cagccgccgc tcctcatcga tattatcttc
    37621 atcgatgctg ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt agacataagg
    37681 aaactccagc ccttttgacg cgtgcagagt catcagttgc acctgatcca gctcttcttc
    37741 actctcacca cgctccatca tgtcgcgcaa agtaaagcgc gtcaccacct gggtgagcgt
    37801 catcggctca tccagttcgc tgccttccag catctccgtc atccagctaa acagttggtt
    37861 gacgttcttc atgcgcattt cggcggcttt cgggctgggc gatgtttcgt acagccagga
    37921 ttcataatcc atgccgtgga tcagatcgcg caccgcggca atcggctccc gctccgccag
    37981 acgctggatt tctgccaacc agtgagtaaa gcgggtcaat gcttcataac cacgtccgct
    38041 aagcgtctga ctcaggccca tatcaaagct ggcggtaaac atgcttttat tgcgcgtcat
    38101 cgcccactca cccagctttt tcagcgtcgc cgggccaatc tctctcttcg gcgtgttaac
    38161 gatacgcaga aatgcgctgt catcgtccgg gttagtcagc acgcgcagat aagccagcaa
    38221 gtccttgatt tcaggacgag agaaaaacga cgtaccacca gaaattttgt acgggatgcg
    38281 gttttgcatc aggaattttt caaacactcg cgactgatgg ttaccgcgat aaagaatagc
    38341 gtaatatttg tactgcgttt tattgacgaa gtgatgcgca atcagttcac cagtgacgcg
    38401 ctcggcttca tgttcttcgt tattcgcgct taaaacttta agctctgtgc cgtaacccag
    38461 ttcggagaac agacgcttct caaagacgtg cgggttattg gcgatcagga tgttcgccgc
    38521 tttcaaaata cgtccggaag agcggtagtt ctgctcaagc ttaatcacct tcagcgccgg
    38581 aaaatcctga ctcagcagca ccaggttttg tggacgtgcg ccgcgccagg agtagatcga
    38641 ctggtcgtca tcgcccacca cggtaaagcg cgcgcggctg cccaccagca gtttcaccag
    38701 ttcatactgg ctggtgttgg tgtcctgata ctcatccacc agcagatagc gaattttgtt
    38761 ctgccagcgc tcacggactt cttcattgcg ttgcagcagc aacgtcggca ataaaatcag
    38821 atcatcgaag tcgagaacat tgcacgcttt caggtgtgca tcatacagcc cataacaatg
    38881 ggcaaaaata cggtcccgct cgccaatcgc actggctgcc gcctgggacg gtgttttgag
    38941 atcgttcttc cagttagaga tggtcgaaat caactgttgc aggagaactt tgtcatcttc
    39001 aatcagccct tcggtcaact ctttgagcaa cgccagctga tcggtatcgt caaacagcga
    39061 aaagttcgcc ttcatcccca gcgccgcata ctcacgcttg atgatatcca gccccagcgt
    39121 gtggaaggtt gagatcatca gcccacgcgc ctctttgcga cccagcgtct gtccgacacg
    39181 ctctttcatc tcgcgcgccg ccttattagt aaaggtcacc gccgcgatat gtcgcgcctg
    39241 ataaccgcaa ccgcggatca gatgggcgat tttattggtg atcacacgag ttttaccgga
    39301 acccgcgccc gccagcacca ggcaggggcc ggtaacgaat tcgacagctt gttgttggcc
    39361 ggggtttaga cgcataggtg tattgctcaa tcttcgaacg gggggaggat tgtagcagaa
    39421 tcgcatttga atgtgaccgc gtgtaagccg ctaaccggtt ggctgcggat gtttaaatca
    39481 gagagggaag accgaaaccg gagttcatcg tgaatccatt agacttagaa aatatcgggt
    39541 cgtaacgttg tgttattact ttttcagagt ttactaagga gaggtgtact cgtggcgatt
    39601 aatttcagtc ctaaggttgg tgaaatactg gaatgcaact ttggtaacta tcctgttagc
    39661 cagaatggac agttcagcac cacgtattac gatggccgca ttcctcctga aatgataaaa
    39721 aacag
//
LOCUS       DAJVOY010000015        37528 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVOY010000015 DAJVOY010000000
VERSION     DAJVOY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.015"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1984
                     /locus_tag="OQL90_000707"
     CDS             <1..1984
                     /locus_tag="OQL90_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCS1367582.1"
                     /translation="VGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDT
                     FNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVN
                     QVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHN
                     TPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDI
                     NDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILT
                     NQTNLTSTFYTGSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTR
                     NGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCP
                     AGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQS
                     GSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQY
                     GKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLW
                     SQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLN
                     VYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            2026..2703
                     /gene="ybiX"
                     /locus_tag="OQL90_000708"
     CDS             2026..2703
                     /gene="ybiX"
                     /locus_tag="OQL90_000708"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1367583.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2777..3043
                     /gene="ybiI"
                     /locus_tag="OQL90_000709"
     CDS             2777..3043
                     /gene="ybiI"
                     /locus_tag="OQL90_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1367584.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3308..3568
                     /locus_tag="OQL90_000710"
     CDS             3308..3568
                     /locus_tag="OQL90_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1367585.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3796..4881)
                     /gene="hcxB"
                     /locus_tag="OQL90_000711"
     CDS             complement(3796..4881)
                     /gene="hcxB"
                     /locus_tag="OQL90_000711"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1367586.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGYLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5022..5984)
                     /gene="ybiB"
                     /locus_tag="OQL90_000712"
     CDS             complement(5022..5984)
                     /gene="ybiB"
                     /locus_tag="OQL90_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1367587.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6012..8162)
                     /gene="dinG"
                     /locus_tag="OQL90_000713"
     CDS             complement(6012..8162)
                     /gene="dinG"
                     /locus_tag="OQL90_000713"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1367588.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8282..8764
                     /gene="ybiA"
                     /locus_tag="OQL90_000714"
     CDS             8282..8764
                     /gene="ybiA"
                     /locus_tag="OQL90_000714"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1367589.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8996..10360)
                     /gene="rhlE"
                     /locus_tag="OQL90_000715"
     CDS             complement(8996..10360)
                     /gene="rhlE"
                     /locus_tag="OQL90_000715"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCS1367590.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10589..11260
                     /gene="cecR"
                     /locus_tag="OQL90_000716"
     CDS             10589..11260
                     /gene="cecR"
                     /locus_tag="OQL90_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1367591.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11263..12258
                     /gene="hlyD"
                     /locus_tag="OQL90_000717"
     CDS             11263..12258
                     /gene="hlyD"
                     /locus_tag="OQL90_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1367592.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12251..13987
                     /locus_tag="OQL90_000718"
     CDS             12251..13987
                     /locus_tag="OQL90_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1367593.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13980..15113
                     /locus_tag="OQL90_000719"
     CDS             13980..15113
                     /locus_tag="OQL90_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1367594.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15124..16230
                     /locus_tag="OQL90_000720"
     CDS             15124..16230
                     /locus_tag="OQL90_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1367595.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16192..16602)
                     /locus_tag="OQL90_000721"
     CDS             complement(16192..16602)
                     /locus_tag="OQL90_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1367596.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16735..17486
                     /locus_tag="OQL90_000722"
                     /pseudo
     CDS             16735..17486
                     /locus_tag="OQL90_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17483..18724
                     /gene="clsB"
                     /locus_tag="OQL90_000723"
     CDS             17483..18724
                     /gene="clsB"
                     /locus_tag="OQL90_000723"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1367597.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18724..19680
                     /locus_tag="OQL90_000724"
                     /pseudo
     CDS             18724..19680
                     /locus_tag="OQL90_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(19716..20429)
                     /locus_tag="OQL90_000725"
     CDS             complement(19716..20429)
                     /locus_tag="OQL90_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1367598.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20635..21339)
                     /locus_tag="OQL90_000726"
     CDS             complement(20635..21339)
                     /locus_tag="OQL90_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1367599.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21476..21928)
                     /gene="moaE"
                     /locus_tag="OQL90_000727"
     CDS             complement(21476..21928)
                     /gene="moaE"
                     /locus_tag="OQL90_000727"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1367600.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21930..22175)
                     /gene="moaD"
                     /locus_tag="OQL90_000728"
     CDS             complement(21930..22175)
                     /gene="moaD"
                     /locus_tag="OQL90_000728"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1367601.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(22168..22653)
                     /gene="moaC"
                     /locus_tag="OQL90_000729"
     CDS             complement(22168..22653)
                     /gene="moaC"
                     /locus_tag="OQL90_000729"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1367602.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22656..23168)
                     /gene="moaB"
                     /locus_tag="OQL90_000730"
     CDS             complement(22656..23168)
                     /gene="moaB"
                     /locus_tag="OQL90_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1367603.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(23190..24179)
                     /gene="moaA"
                     /locus_tag="OQL90_000731"
     CDS             complement(23190..24179)
                     /gene="moaA"
                     /locus_tag="OQL90_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1367604.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(24167..24311)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24576..25484
                     /gene="yvcK"
                     /locus_tag="OQL90_000732"
     CDS             24576..25484
                     /gene="yvcK"
                     /locus_tag="OQL90_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1367605.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(25676..27697)
                     /gene="uvrB"
                     /locus_tag="OQL90_000733"
     CDS             complement(25676..27697)
                     /gene="uvrB"
                     /locus_tag="OQL90_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1367606.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(28276..28953)
                     /gene="bioD"
                     /locus_tag="OQL90_000734"
     CDS             complement(28276..28953)
                     /gene="bioD"
                     /locus_tag="OQL90_000734"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1367607.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(28946..29701)
                     /gene="bioC"
                     /locus_tag="OQL90_000735"
     CDS             complement(28946..29701)
                     /gene="bioC"
                     /locus_tag="OQL90_000735"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1367608.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(29688..30842)
                     /gene="bioF"
                     /locus_tag="OQL90_000736"
     CDS             complement(29688..30842)
                     /gene="bioF"
                     /locus_tag="OQL90_000736"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1367609.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(30839..31879)
                     /gene="bioB"
                     /locus_tag="OQL90_000737"
     CDS             complement(30839..31879)
                     /gene="bioB"
                     /locus_tag="OQL90_000737"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1367610.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            31966..33255
                     /gene="bioA"
                     /locus_tag="OQL90_000738"
     CDS             31966..33255
                     /gene="bioA"
                     /locus_tag="OQL90_000738"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1367611.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            33314..33790
                     /locus_tag="OQL90_000739"
     CDS             33314..33790
                     /locus_tag="OQL90_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1367612.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            33942..35225
                     /locus_tag="OQL90_000740"
     CDS             33942..35225
                     /locus_tag="OQL90_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1367613.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35366..>37528)
                     /locus_tag="OQL90_000741"
     CDS             complement(35366..>37528)
                     /locus_tag="OQL90_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1367614.1"
                     /translation="KGTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKA
                     SGMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAIIHQYMR
                     EMMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGK
                     PAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLS
                     SVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVY
                     EIDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYEN
                     VIAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCF
                     GAGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSAS
                     ELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKV
                     CSKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLA
                     GNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLA
                     NIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVI
                     HEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         8795 a        10167 c         9725 g         8841 t
ORIGIN      
        1 cgtgggtgcg tttttggcgg gtgagaacgg taactccacc actggcgacg ccatttatat
       61 gcgtggtgcc gatacctcta acagtattta tattgatggc attcgcgata tcggcagcgt
      121 ctcgcgcgac accttcaata ccgagcaggt cgaagtgatt aaagggccgt ccggcaccga
      181 ctacgggcac agcgcaccga cgggctcgat caatatgatc agcaaacagc cgcgcaatga
      241 ttccggcatt gacgcctccg ccagtattgg cagcgcctgg ttccgccgcg gcacgctgga
      301 cgtcaatcag gtcattggtg ataccaccgc ggtgcgcctg aatgtaatgg gcgaaaaaac
      361 gcacgatgcc ggacgcgaca aagtcaaaaa tgagcgttac ggcgtcgccc cttctgtcgc
      421 ttttggcctt ggtacagcga atcgtttgta tcttaattat ctgcatgtca cccagcacaa
      481 cacgccagac ggcggcattc cgaccatcgg tttgccgggc tattctgccc catctgcggg
      541 aacggcggcc ctgaatcatt ccggaaaagt tgatactcat aacttttacg gcacggattc
      601 cgattacgac gattcgacca ccgacaccgc caccatgcgt tttgagcacg acatcaacga
      661 taacaccacc attcgcaata ctacccgttg gtcgcgcgta aagcaggatt acctgatgac
      721 ggcgattatg ggcggggcgt cgaatattac tcagcccacc agcgatgtga atagctggac
      781 ctggtcacgc acggcgaata ccaaagatgt gagtaataaa attctcacca accagaccaa
      841 cctgacctcg acgttctata ccggttctat cggtcatgat gtcagtaccg gcgtggaatt
      901 tacccgtgaa acgcagacta actacggcgt taatccggtg acgttacccg cggtaaatat
      961 ttatcatcct gacagcagca ttcatcccgg cggcctgacg cgcaacggcg caaacgccaa
     1021 tggtcagacg gataccttcg caatttacgc ctttgatacg ctgcaaatca cccgtgattt
     1081 tgagctgaac ggcgggatcc gtctggataa ttatcatact gaatatgaca gtgccaccgc
     1141 ctgcggcggc agcggacgcg gtgccatcac ctgcccagct ggtgtggcaa aaggttctcc
     1201 ggtcaccacc gtcgacaccg ccaagtcggg caatctggtg aactggaaag ccggggcgct
     1261 gtatcacctg acggaaaacg gtaatgtcta tattaactat gccgtttccc agcagcctcc
     1321 gggcggcaac aacttcgccc ttgcgcagtc tggcagcggt aacagtgcca accgcaccga
     1381 ttttaaaccg caaaaagcca acaccagcga gattggcacc aaatggcagg ttctggataa
     1441 acgcctgttg ctcaccgccg cactgttccg tactgatatc gaaaatgaag ttgagcaaaa
     1501 tgatgacgga acttactcgc aatacggtaa gaaacgtgtc gaaggctatg agatatccgt
     1561 ggccgggaat atcactcccg cgtggcaggt gattggcggc tatacccagc aaaaagcaac
     1621 catcaaaaac ggcaaagatg ttgcccagga tggttcctca tcgctgccgt ataccccgga
     1681 gcacgccttc accttatgga gccagtatca ggcaaccgat gatatctctg ttggcgcggg
     1741 cgcacgctat atcggcagta tgcataaagg ttcagacggc gcggtgggaa cgccagcgtt
     1801 taccgaaggt tactgggtcg ccgatgccaa actggggtat cgggttaatc gcaatctcga
     1861 cttccagcta aacgtttaca acctgtttga taccgattac gtcgcctcaa tcaacaagag
     1921 cggctaccgt tatcacccgg gcaagccaag aaccttcttg ctcacagcca atatgcattt
     1981 ctgattcaga tgtggggcgc aggccccact ttttggagaa attgtatgat gtaccacatt
     2041 cccggcgtgt tatcgccaca ggacgtcgct cgttttcgcg aacaactgga acaagccgaa
     2101 tgggtggatg gacgcgtcac caccggcgca caaggtgcgc aagttaagaa caatcaacag
     2161 gtagacaccc gcagcacgtt atacgccgcc ctgcaaaatg aggtgctgaa cgcggttaac
     2221 caacatgctt tattctttgc cgcggccttg ccgcgtaccc tttccacgcc gctgtttaat
     2281 cgctatcaga acaatgaaac ctatggtttt catgtggatg gcgcagtacg cagccatcca
     2341 caaaacggct ggatgcgcac tgacctttct gccacgctgt ttttaagcga tccacaaagc
     2401 tacgacggcg gcgaactggt cgttaatgac accttcggac aacatcgggt aaaactcccg
     2461 gcaggtgatc tcgtgttgta tccctccagc agcctgcatt gcgtgacacc cgtaacccgc
     2521 ggcgtacgag tggcatcatt tatgtggatc cagtcgatga tccgcgatga taaaaagcgc
     2581 gccatgctgt ttgaactaga caaaaatatt cagtcgctga aaagccgcta tggtgaaaat
     2641 gaagagatcc tgtcgctgct caatctttat cataatctgc tgcgggaatg gtcggagatc
     2701 tgacgcttga actgcccagg ttcgtcccta tattagctac acttaactgt acaagcattg
     2761 atatggggag gacgatatgg catccggttg ggctaacgat gacgccgtca acgaacagat
     2821 caacagtaca attgaagatg cgattgcccg cgctcggggt gaaattccgc gtggcgaaag
     2881 cctgtatgaa tgtgaagagt gcggtgcccc catcccgcaa gcccgtcggg aagccattcc
     2941 tggcgtgcgc ttatgtattc attgtcagca ggagaaagat ttacaaaaac cagcgtatac
     3001 aggatataat cgcagaggtt cgaaagacag ccagttacgt taactacaac tggcgagatg
     3061 catagcgagt ataatttgta ttttgcgtgg cattccgtgc ctttaacgct gctgtgactg
     3121 ccacgcggca taaaacaagt ccataagtac aacaaataaa tggtttatca gtatgttaga
     3181 tgtcaatcaa tttatttgaa caaggcggtc aattctcttc gattttatct ctcgaaaaaa
     3241 aacgtgatac tcatcacatc gacgaaacaa cgtcacttat acaaaaatca cctgcgagag
     3301 attaattatg aaaactatca atactgttgt tgctgctatg gctctttcaa ctctatcatt
     3361 tggcgtgttc gcggcggaac cggtaacggc atcccaggca cagaacatga ataaaatcgg
     3421 cgtggtttct gccgatggcg catccaccct cgatgccctg gaagcgaaac tgactgagaa
     3481 agccgcggca gccggtgcca gtggatacag catcacttct gccaccaaca acaataaatt
     3541 aagcggtact gcggtaattt ataagtaatt cgtaggacgg ataaggcgtt tacgccgcat
     3601 ccggcaacag atgcctgatg cgacgctacc gcgtcttatc aggcctacaa aatccgaacc
     3661 gttggacgga tcaggcggtt acgctacgtc cagcacaagt atttccctgt ctgtttgccg
     3721 acagacgcat atgctctaac cctcattgat cctatattac ccttgtttgc ccgtccgtta
     3781 ctagacgggc tttttttagc tggctaactg ctgacagaaa gcctgcaacg tctcttccga
     3841 cataccaatc tgccgcgccg catcacaaat ggcctgccag cttcccgcat ccaatggaat
     3901 cccctgctcc tgccgttcgc gacgcgtgtt cacttcccac tcgcccggta gcaaaatcgg
     3961 cttatcgtca tcatgcggcg aggctttcac ccactcggca aaggcttcag tctgcgcgct
     4021 acaatccggc gcgccgaaca gttccgggtt gatgatgata gtggtcatgc agttaagaat
     4081 ggcatcggga ctggtttgta acgtttcctg atgcgtcgtt ttaccgccag aaagcgcccc
     4141 gccaagaatt tcacacatcg ctgcaagggc gtagccttta tgttcggcaa aggtcaacag
     4201 cgaccccaac ggcgactcct gcattaccgc cggattggtc gtcggcacgc cgttaacgtc
     4261 aatcaggcaa cctggcggca cggggacgcc tttatgccag gcgacgcgag ttttaccaaa
     4321 tgcgatggcg ctggtggcgt aatcaagcaa cagcgggaaa ttatctttac gagggaaaac
     4381 cacacagaac ggattggtgc caaagcggct gtcgcgaccg tggaacggtg cgaccattgg
     4441 aataccgacc acgctaacaa agtggataga gacaaacccc gccgctgcac actgctccgc
     4501 ccagtaaccg atacggccga tatgatgcga gttatgtagc gccacggcag caataccgtg
     4561 ctgatgcgct ttctcaatcc ccagcgccat cgcttcgtgt gccgcgacct gaccgaatgc
     4621 gcgatcgcca tcgagcgtga ctgccgcccc cgcctcttta acgattttgg catgatggtt
     4681 aatttgcaga tacccctgac tccaggaacg cacatagctt gggatcatgc caataccatg
     4741 agaatcatgc cctgccaggt ttgccgcgat taaatgatcg gccactaatt tcgcttcttg
     4801 ttcctcgcta cccatctgac gaaatacagc ctgaataaaa ctgtgcagcg tctgagcatc
     4861 aaagcgatga ccactttcca tggttaactc ctgtttatgt tatgtgtttg ttgttttttt
     4921 atgctgcacg ccgggcgcgg gaaggtcaat aaagatgcag gaatttcata ttgcggtggg
     4981 gtaaaagccg gatgacatgg ctcatccggc tgagaaaaga attaaaatgc ctgattaacg
     5041 cgcgccaggc cgtcgctgat agttgccgct tcacccgtag cctccaggca gcaagccatc
     5101 tggattttca gcgattcggg aatcggttcg ctgccagcaa ggcaacgctc aatccactgc
     5161 gccgtggttt ctgggtcttt tgcttgtggc agtaactcgc taccagcagt gtcctgtttt
     5221 tcatacagca cccgcatccc ttcacggtca atgagattga tctgcgggca gcgctgcgga
     5281 ttagcataca cttcgccttc agtaccatgc attagtagcg cccgcccgcc gatatcgcta
     5341 aagaacttcg cgactcgccc gatgtattcc ggatgcgaaa cgctggaaag acgcagcgcc
     5401 tcaccttcgg caaatggcgt cgccagtttc gccagggtat gtgcactgtt acgcacgccc
     5461 atccgccagc gcatcgccag ttgtttttcc agcggcgggc aaaacgcgcc gacaggcata
     5521 aacaccggtt gatgttcgtc gagcttcgcc tgcgcctgcc cgccgtgcaa cgttggtgta
     5581 atacccatca gttcgaaaat ggtttctgtc agcacgcgag ttggatcttc gctaaccccg
     5641 tgaaccacca caggaaaacc gagtttgtgc aggagaatag ccaacaacgg cgtcaggttg
     5701 gcctgtttac gcgcgccgtt gtaactgggg atgacaatcg gcatcggctt gcctgcaggc
     5761 ggcgtcagct tgatggtgtg attttgcatg gcttcgtaaa agccgagcat ctctgcttcc
     5821 ccttcccctt tgatacgtag cgcaatcaat acgccgccca actcaaggtc agggacttca
     5881 ccattgagca tatgggcata cagaccgcgc gcagtatccc ggtctaaatc gcgcgcgtgg
     5941 tttttcccgc gcccgatctc tttaatgatt ttgcgatagt ccatttacga ctccttacct
     6001 gactcacatc attaacgccg ccgacggcgt ggggatttcg ttttttcttt ctttttaact
     6061 ataccttcag ggacttccgg ttgctctatc ggaaataccg gtaatgcatc cagtagtcgc
     6121 ttgccataat ttttggtcag caagcgtttg tcgtagataa ccacttcgcc ccagcaaccg
     6181 tggcttcgaa tcagtcgccc aacctgctga atcaggttaa acgaggcgct cggcaggctt
     6241 tgcacctcaa acggatagcg gttgaggctt ttcagccatt ccccttcggt gatcaccacc
     6301 gggctgtcga tgggcggaaa agcgattttg tggatatgca cctggctgag cagatcacct
     6361 ttcaaatcaa gcccttcggc aaatgactgt aagcccacca gcacactacg ctcaccgttg
     6421 gcaacgcgtt tgcggtgcag ttcaactaag cgataacgcg gctgatcgcc ctgcaccagc
     6481 agcatcaggc gtaaatccgt cacatagtcg agaaagcgct gcatcgcccg tccgctggca
     6541 aacagtacca acataccgag atgttttttg ctctccacct gctcacggaa aaaggccgcc
     6601 atttcggcaa tatgctgctc ttcgttgtcg atggaaggct caaagcgcat ccggggaata
     6661 acaattttgc cctgttcgca gtggttaaag ggggaatcca gcgccacaaa acggtcgccc
     6721 gctttctctt tcagcccgct catctcctgc aaacgcgaaa aactgttcag cgaacgcaag
     6781 gttgcggagg tgacaataat gtgcggaata ctgcgccaca gcagcctttc cagctgatcg
     6841 ctgacacgta ttcccacgca gtgaaaccag agatgcagct gcccttcgcg ctcttcccgc
     6901 gtcgcccatt tggtcaccgg cgcaccggaa gattgcgcca gagacgccag ccgccagagt
     6961 ttgctttgcg cctcgaacat ccccaacgcg cggttcatct gcaaaatcaa ccgatgcaga
     7021 cgcacaatat cgtggctgcc ggttttctca cttaaatcgt taagaaataa ctccgccaga
     7081 ccacgcagca tctcggtgag ttttgccagc cgctggcaga tctccagcac ttcatcaggc
     7141 agttcgccca tcgcaaaacg gtgctctgcc tcctgcccgg caggcatgta gagattgaga
     7201 atgttgttta acgaggcgat aagctcatac aactcttcac aatgcgcatt caaacgttca
     7261 gggatcgcca gcggtgggat ggtcttcggg cgaaactgct ccatgcaggt agcgaccagt
     7321 ttcgtgaaca agtccagctg tagtcgatac cagggcgcgg tgatttcagc gctcatctcc
     7381 agcgcatccc gggccacatc tggcaggtga tgaccttcgt ccagcaccag cagcagattt
     7441 ttcgggtcag gcagtacggc ttcgctttcc atcgccgcca tcaccagcgc atggtttgcc
     7501 accaccactt ccgcttcctg aatctcccga cgagcaacaa aaaacgggca ttcacgatag
     7561 tagtaacagt tacggttaag gcagctggct ttgtcggtac tcaaacgacg ccagagatca
     7621 tcatctatag cgatatccgt atgatcgcgc agaccatccc atttataagt gtcgagatcg
     7681 cccttcagct tcgcacaacg tttttgctct tcctgattat ttggcgtcag ttcgtcgtca
     7741 agaaacgcca gcagatcctg ttgcgtgggt tcagtgctgg cgagcgccgt cagattacgc
     7801 ggacaaacgt agcgcccacg cccgaaagcg gcagtgaatt taagatcggg aatgatcttt
     7861 ttcagcagcg gtaaatcttt gctgtaaatc tgatcctgca acgccacgtt ggcggtactc
     7921 accaccagcg ttttttgctc ttcgcgggca atggcgatgc cggggatcaa ataggagagc
     7981 gttttcccaa cgccggtggg ggcttcaatc gccagatgcc gcccttcttc tccggccagc
     8041 gttttggcga cgtccgcaat catctgccgc tgcggcgcac ggggaataaa gtcggggatc
     8101 tgttcctgaa gcgccttata ccaggcggca atttgcgctt taagcgcggc ggttaatgcc
     8161 atgagaaaac ctgaaatact gtataaacag ccaatattgt ggcatttttc ggttttcagg
     8221 ggtaatggtt tttctgtttc tggaaagcgg ctcgcaataa ccaccaacat aatgcggttt
     8281 aatgcccgtt cgagcacaaa ggatccaaca cgtcatgcaa gacaccatca taaatttcta
     8341 cagcaccagc gatgactacg gtgatttttc taactttgcc gcctggccca tcaaggttga
     8401 cgggaaaacc tggcccacct ccgaacacta ttttcaggca caaaaattcc ttgatgaaaa
     8461 ataccgcgaa gagatccgtc gggtttcttc ccccatggtc gccgctcgca tggggcgtaa
     8521 tcgttccaag ccacttcgta aaaactggga gtcggttaaa gaacaggtga tgcgtaaagc
     8581 acttcgtgct aaattcgaac agcatgcaga actgcgtgcg ctcttgctgg caaccgcgcc
     8641 cgcaaaactg gttgagcata cggaaaacga tgcttactgg ggagacggtg gtaatggtaa
     8701 gggcaagaat cgactgggct accttttaat ggagttgcgc gaacaattgg ctatagagaa
     8761 gtaacgtctc ggtggcttat gggcggtaaa acagataaat tttttcccgt gttctcaaat
     8821 tgctgacaag tgcttgttgt gaaatgccgg atgcggcgta aacgccttat ccggcctaca
     8881 aaagcatgca aattcaatat attgcagagt taacgtaggc ctgataagcg tagcgcatca
     8941 ggcagttttg cgtttgttca tcagcctgat gccgggcata gcccggcata aaagattact
     9001 gcgcagcggc aggtttacgc ggacggcggc gacgttgttg ttcgcctgcc ggtttggcat
     9061 cgccgaggcg gcgagacggt ttttctgcag gtttcgcgct tgcagatttt gcgccacctt
     9121 ccccacggcg tggttgctgt tgaccgcgac caccaccttg cccacgaccg ccgccgccac
     9181 gttgctggcg accgttctgg atcggttcgg ctttgattga cgggtccggc tcatagcccg
     9241 gaatcgcaat gcgcgggatc tcttttttca gcagtttttc gatatcacgc agcagtttgt
     9301 gttcatcaac acacaccagc gacaacgctt caccggtagc agccgcacga ccggtacgcc
     9361 cgatacggtg gacataatct tccggtacgt ttggcagttc atagttgacg acgtgcggca
     9421 actcttcgat atccagaccg cgcgcagcga tatcggtcgc caccagtacg cgaatgtcac
     9481 tcgatttaaa atcagccagc gcacgggtac gcgcaccttg cgatttattc ccgtggatcg
     9541 ccgcactacg gatgccatct ttattgagct gctcagccag atggttagcg ccgtgtttgg
     9601 tacgggtaaa caccagcacc tgctgccagt tccctttacc aatcatgtgc gacagcaatt
     9661 cgcgtttgcg tttcttatcg acaaagtgaa cgtgctgggt cacctgatca gacgcggtat
     9721 tgcggcgtgc cacttcgatt tccagcgggt tgtgcaacag tttttccgcc agggctttaa
     9781 tatcgtcaga gaaggtcgca gagaacagca ggttctggcg tttcgctggc agttttgtta
     9841 acacgcgacg gatatcgtgg ataaagccca tgtcgagcat gcggtcagct tcatcgagga
     9901 cgaggatttc aacctgatcc agcttcactg cattttgatg ttccaggtcc agcaaacgtc
     9961 ccggtgttgc caccagaaca tcaacgccgc cgcgcagctt catcatttgc gggttaatgc
    10021 tgacaccgcc aaacaccacc agcgaacgaa tgttcaaata tttgctgtaa tcacggacgt
    10081 tttcgccaat ctgcgcagcc agttcacggg tcggggtaag aatgagcgca cgtaccggac
    10141 gacgcccttt ggcgtgcggc tggcgagtga tcaggtgttg caacagcggc agcgtaaagc
    10201 ccgctgtttt accggtaccg gtctgggcgc tagccatcag gtcgcggcct tccagcaccg
    10261 cagggatcgc ctgctgctga ataggggtgg gttcacggta accctgctcg gcaacggcgc
    10321 gcaggatatc agggcttaaa cccaaagaat cgaaagacat aactactccg aaccgccccg
    10381 accgttaccg gtgtagtttt cagggagata ctgaaaagga aaatgacaaa aaccacaatg
    10441 tcatgaggga gcggagtgta gcagtttttg tgacgcagcg cataaattat ccctgtgact
    10501 ggcgttgcta aaattcttag ccatactgga caactcccct atggagtcat aatcttaatc
    10561 aatcatttga ttaagaatgg actgtgcgat gaataatcct gccatgacaa tcaagggtga
    10621 acaggcgaaa aaacagctga ttgctgccgc actggcgcag tttggtgaat atggaatgaa
    10681 caccaccact cgcgagatag ccgcccaggc cgggcagaat atcgctgcca tcacctacta
    10741 cttcggttcg aaagaagatt tgtacctcgc ctgcgcccag tggattgccg attttattgg
    10801 cgagcagttc cgtccgcatg ccgaggaagc cgaacgcttg ttcgcgcaac cacagcctga
    10861 tcgggctgcc atccgtgaac tgatccttcg cgcctgcagg aacatgatta agctgctcac
    10921 ccaggatgac accgtcaacc tcagcaagtt tatctcccgt gagcagctct ctcccacggc
    10981 agcctaccac ctggtgcacg aacaggtgat tagtccgcta cacagccacc tgacacgcct
    11041 gattgccgcc tggaccggct gcgacgccaa tgacacccgc atgatccttc atacccatgc
    11101 gttgattggt gagattctgg cgttccgtct tggcaaagaa acaattctgt tacgtaccgg
    11161 ctggaccgcg ttcgatgaag aaaagaccga actgatcaac cagacggtga cctgtcatat
    11221 cgacctgatt ttgcaaggat tatcgcaaag gagtttgtag tgatgaaaaa acctgtcgtg
    11281 atcggactgg cggtagtggt acttgccgcc gtggttgccg gaggctactg gtggtatcaa
    11341 agccgccagg ataacggcct gaagctgtat ggcaacgtgg atattcgtac ggtgaatctt
    11401 agtttccgtg ttggggggcg cgttgaatcg ctggcggtgg acgaaggtga tgcgatcaaa
    11461 gcgggccagg tgctgggcga actggatcac aagccgtatg agattgccct gatgcaggtg
    11521 aaagcgggcg tctcggtggc acaggcgcag tatgacctga tgcttgccgg gtatcgcgat
    11581 gaagaaatcg ctcaggccgc cgcagcggtg aaacaggcgc aagccgccta tgactatgcg
    11641 cagaacttct ataaccgcca gcaaggactg tggaaaagcc gcaccatttc ggcaaatgac
    11701 ctggaaaatg cccgctcctc gcgcgaccag gcacaggcaa cgctgaaatc agcacaggat
    11761 aaattgcgtc agtaccgttc cggtaaccgt gaacaggaca tcgctcaggc gaaagccagc
    11821 ctcgaacagg cgcaggcgca actggcgcag gcggagctga atttacagga ctcaacgttg
    11881 atagcaccgt ctgatggcac actgttaacg cgcgcggtgg agccaggcac ggttctcaat
    11941 gaaggtggca cggtgtttac cgtttcacta acgcgtccgg tgtgggtgcg cgcttatgtt
    12001 gatgaacgga atcttgacca ggcccagccg gggcgcaaag tgctgcttta taccgatggt
    12061 cgcccggaca agccgtatca cgggcagatt ggtttcgttt cgccgactgc tgaatttacc
    12121 ccgaaaaccg tcgaaacacc ggatctgcgt accgacctcg tctatcgcct gcgtattgtg
    12181 gtgaccgacg ccgatgatgc gttacgccag ggaatgccgg tgacggtgca attcggtaac
    12241 gaggcaggac atgaatgatg ccgttatcac gctgaacggc ctggaaaaac gctttccggg
    12301 catgggcaag cccgccgtcg cgccgctcga ttgtaccatt cacgccggtt atgtgacggg
    12361 gctggtgggg ccggatggtg caggtaaaac cacgctaatg cggatgctgg cgggattact
    12421 gaaacccgac agcggcaatg ccacgatgat tggctttgat ccaatcaaaa acgacggcgc
    12481 gctgcacgcc gtgctcggct atatgccgca gaaatttggt ctgtatgaag atctcacggt
    12541 gatggagaac ctcaatctgt acgcggattt gcgcagcgtc accggcgagg cacgtaagca
    12601 aacttttgct cgcctgctgg agtttacgtc tcttgggccg tttaccggac gcctggcggg
    12661 caagctctcc ggtgggatga aacaaaaact cggtctggcc tgtaccctgg tgggcgaacc
    12721 gaaagtgttg ctgctcgatg aacccggcgt cggcgttgac cctatctcac ggcgcgaact
    12781 gtggcagatg gtgcatgagc tggcaagcga agggatgtta atcctctgga gtacctcgta
    12841 tctcgacgaa gccgagcagt gccgtgacgt gttactgatg aatgaaggcg agttgctgta
    12901 tcagggggaa ccaaaagccc tgacacaaac catggccgga cgcagctttc tgatgaccag
    12961 tccacacgag ggcaaccgca aactgttgca acgcgccttg aaactgccac aggtcagcga
    13021 cggcatgatt caggggaaat cggtacgtct gatcctcaaa aaagaggcca caccagacga
    13081 tattcgccat gccgacggga tgccggaaat caacatcaac gaaactacgc cgcgttttga
    13141 agatgcattt attgatttgc tgggtggtgc cggaacctca gaatcgccgc tgggtgcaat
    13201 attgcatacg gtagaaggca cacccggcga gacggtgatc gaagcgaaag aactgaccaa
    13261 gaaatttggt gattttgccg ccaccgatca cgtcaacttt gccgttaaac gtggggagat
    13321 ttttggtttg ctggggccaa acggcgcggg taaatcgacc acctttaaga tgatgtgcgg
    13381 tttgctggtg ccgacttccg gccaggcgct ggtgctgggg atggatctga aagagagttc
    13441 cggtaaagcg cgccagcatc tcggctatat ggcgcaaaaa ttttcgctct acggcaacct
    13501 gacggtcgaa cagaatttac gctttttctc tggtgtgtat ggcttacgcg gtcgggcgca
    13561 gaacgaaaaa atctcccgta tgagcgaggc atttggcctg aaaagtatcg cctcccacgc
    13621 gaccgatgaa ctgccattag gttttaaaca gcggctggcg ctggcctgtt cgctgatgca
    13681 tgaaccggac attctgtttc tcgacgaacc aacgtccggc gttgatcccc tcacccgccg
    13741 cgaattttgg ctgcatatca acagcatggt agagaaaggc gtcacggtga tggtcaccac
    13801 tcactttatg gatgaagcag aatattgcga ccgcatcggc ctggtgtacc gtgggaaatt
    13861 aattgccagc ggcacgccgg atgatttgaa agcgcagtcg gccaacgacg agcaacccga
    13921 tcccaccatg gagcaagcct ttattcagtt gatccacgac tgggataagg agcatagcaa
    13981 tgagtaaccc gatcctgtcc tggcgtcgcg tacgggcgct gtgcgttaaa gagacgcggc
    14041 agatcgttcg cgatccgagt agctggctga ttgcggtagt gatcccgctg ctactgctgt
    14101 ttatttttgg ttacggcatt aacctcgact ccagcaagct gcgggtcggg attttactgg
    14161 aacagcgtag cgaagcggcg ctggatttca cccacaccat gaccggttcg ccctacatcg
    14221 acgccaccat cagcgataac cgtcaggaac tgatcgccaa aatgcaggcg gggaaaattc
    14281 gcggtctggt ggttattccg gtggattttg cggaacagat ggagcgcgcc aacgccaccg
    14341 caccgattca ggtgatcacc gacggcagtg agccgaatac cgctaacttt gtacaggggt
    14401 atgtcgaagg gatctggcag atctggcaaa tgcagcgagc ggaggacaac gggcagactt
    14461 ttgaaccgct tattgatgta caaacccgct actggtttaa cccggcggcg attagccagc
    14521 acttcattat ccccggtgcg gtgaccatta tcatgacggt catcggcgcg attctcacct
    14581 cgctggtggt ggcgcgagaa tgggaacgcg gcaccatgga ggctctgctc tctacggaga
    14641 ttacccgcac ggaactgctg ctgtgtaagc tgatccctta ttactttctc gggatgctgg
    14701 cgatgttgct gtgtatgctg gtgtcagtgt ttattctcgg cgtgccgtat cgcgggtcgc
    14761 tgctgattct gttttttatc tccagcctgt ttttactcag taccctgggg atggggctgc
    14821 tgatttccac gattacccgc aaccagttca atgccgctca ggtcgccctg aacgccgctt
    14881 ttctgccgtc gattatgctt tccggcttta tttttcagat cgacagtatg cccgcggtga
    14941 tccgcgcggt gacgtacatt attcccgctc gttatttcgt cagcaccctg caaagcctgt
    15001 tcctcgccgg gaatattcca gtggtgctgg tggtaaacgt gctgtttttg atcgcttcgg
    15061 cggtgatgtt tatcggcctg acgtggctga aaaccaaacg tcggctggat tagggagaag
    15121 agcatgtttc atcgcttatg gacgttaatc cgcaaagagt tgcagtcgct gctgcgcgaa
    15181 ccgcaaaccc gcgcgattct gattttacct gtgcttattc aggtgattct gttcccgttc
    15241 gccgccacgc tggaagtgac taacgccacc atcgccatct acgatgaaga taacggcgag
    15301 cattcggtgg agctgaccca acgttttgcc cgcgccagcg cctttactca tgtgctgctg
    15361 ctgaaaagcc cacaggagat ccgcccaacc atcgacacac aaaaggcgtt actcctggtg
    15421 cgtttcccgg ctgacttctc gcgcaaactg gataccttcc agaccgcgcc gttgcagttg
    15481 atcctcgacg ggcgtaactc caacagtgcg caaattgcca ccaactacct gcaacagatc
    15541 gtcaaaaatt atcagcagga actgctggaa ggaaaaccga aacctaacaa cagcgagctg
    15601 gtggtacgca actggtataa cccgaatctc gactacaaat ggtttgtggt gccgtcactg
    15661 atcgccatga tcaccactat cggcgtaatg atcgtcactt cactttccgt cgcccgcgaa
    15721 cgtgaacaag gtacgctcga tcagctactg gtttcgccgc tcaccacctg gcagatcttc
    15781 atcggcaaag ccgtaccggc gttaattgtc gccaccttcc aggccaccat tgtgctggcg
    15841 attggtatct gggcgtatca aatccccttc gccggatcgc tggcgctgtt ctactttacg
    15901 atggtgattt acggtttatc gctggtggga ttcggtctgt tgatttcatc actctgctcg
    15961 acacaacagc aggcgtttat cggcgtgttt gtctttatga tgcccgccat tctcctttcc
    16021 ggttacgttt cgccggtgga aaacatgcca gtatggctgc aaaacctgac gtggattaac
    16081 cctattcgcc actttacgga cattaccaag cagatttatt tgaaggatgc gagtctggat
    16141 attgtgtgga atagtttgtg gccgctactg gtgataacgg ccacgacagg gtcagcggcg
    16201 tacgcgatgt ttagacgtaa ggtgatgtaa cttcttatct ttcgccagca aagacactac
    16261 cgccgggccg gcaaggattg ccagccctgc aattgccagc aaaaagttgt tttccagtac
    16321 gcgggacaac agccacagca caatcagcgc tgcgccaaaa taccaggtcg tggtcagctc
    16381 ttccaacacg tctgccacct cacgccagcg ttgctcgggg tggcgctgaa acaccaacat
    16441 catcactacc gtgacacaat acagtgcgca taatcccacg ccgacgtgaa ccagagtctt
    16501 actcatccag cccaccacca gcagcgccac taccagtaaa tgcaacataa tctgccagca
    16561 acttagaccc gttgcgacac gaacacgttg ttgccacttc atggcttctc tcctgaagaa
    16621 tttatctcta cttactcaga gtaccgaact ttacggaaaa ttccgtaaga ccgcaccttt
    16681 ttttgacgcc gactacacta tttcaatgag ataagaggca aagacaggaa aattatgccc
    16741 gatcaaacac aacaattttc gttcaaggtg ctcaccatca acattcaaaa aggttttacc
    16801 gcgtttaacc gacgcttcat tttgccggaa cttcgcgacg cggtgcgtac cgtcagcgcc
    16861 gatattgttt gcctgcagga agtgatgggc gcgcacgaag ttcatccgct gcatgtggaa
    16921 aactggcccg atacctcgca ctacgagttt ctcgccgaca ctatgtggag cgattttgcc
    16981 tacggtcgca atgccgtata cccggaaggg catcacggca acgccgtact gtcgcgttat
    17041 cccattgaac attatgagaa tcgcgatgtt tcggtcgatg gtgcggaaaa gcgcggcgtg
    17101 ctctactgcc gcattgtgcc gccgatgacc ggaaaagcga ttcatgtgat gtgcgtacat
    17161 ctgggcctgc gtgaggcgca ccgtcaggcg cagcttgcga tggggtgaat gagctaccgg
    17221 acggcgaacc ggtattggtg gcgggtgatt tcaatgactg gcggcaaaaa gctaatcatc
    17281 cgttaaaagt gcaggccgga ctggatgaga tttttacccg cgcccacgga cgcccggcgc
    17341 gcacgtttcc ggtgcaattt cctctactac gactggacag gatctacgtc aaaaatgcca
    17401 gcgccagcgc gccaaccgcg ttgccgctgc ggacatggcg acacctttct gatcatgccc
    17461 ctttaagtgc ggagattcat ttatgaaatg tagctggcgc gaaggcaata agatccagtt
    17521 gctggaaaac ggcgagcaat attatcccgc ggtgtttaag gcgattggcg aggcacaaga
    17581 acgcatcatt cttgaaacgt ttatctggtt tgaggataac gtcggcaaac agctgcatgc
    17641 ggcactactg gcagcagcgc aacgcggggt taaagcggaa gtcttgctgg atggctacgg
    17701 ttcgccggat ctcagcgatg agtttgtcaa taaactgacg gcagctggcg tagtgttccg
    17761 ctactacgat ccccgccctc gcctttttgg tatgcgcacc aatgtgtttc gccggatgca
    17821 tcgcaaaatt gtggtgatcg acgcgcgtat cgcctttatt ggcgggctga attactccgc
    17881 cgagcatatg tccagctacg gtccagaggc taaacaggat tacgcggtac gccttgaagg
    17941 gccgattgtc gaagatattc tccagtttga gctggaaaac ctgcctggac agagcgcggc
    18001 acggcgctgg tggcgacgtc atcacaaagc ggaagagaac cgccagccgg gagaagcgca
    18061 ggtattgctg gtctggcgcg ataacgaaga acatcgcgat gatattgaac gccattatct
    18121 gaaaatgctc actcaggcgc ggcgggaagt gattatcgcc aacgcctact tcttccccgg
    18181 ctatcgattt ttacacgcct tgcgtaaagc ggcacggcgc ggggtgcgga tcaaactgat
    18241 cattcagggc gaaccggata tgccgattgt cagagtcggt gcgcgcttgc tgtataacta
    18301 tctggttaaa ggcggcgttc aggtttttga gtaccgccgc cgcccgctcc acggcaaagt
    18361 ggcattgatg gacgatcact gggcgacagt agggtccagt aatctcgatc cgctcagttt
    18421 gtcactgaat ctcgaagcaa atgtcatcat ccacgatcgt cattttaacc agacgctgcg
    18481 cgataatctg aacggcatta ttgccgcaga ttgtcagcag gtggatgaaa ccatgctgcc
    18541 caaacgcacc tggtggaacc tgaccaaaag cgtgctggcg ttccactttt tacgccactt
    18601 cccggcgctg gttggctggc atccggcaca cacaccacgt ctgacgcagg ttgatccccc
    18661 cgcacaaccg acaatggaaa cgcaggatcg ggtagaaact gaaaacacgg gggtaaaacc
    18721 ctgatgagta aatcacaccc gcgctagcgc ttagcaaaga agatcctcac ctggctgttt
    18781 tttatcgcgg tgattgtgtt gctggtggtc tacgccaaaa aagtggactg ggaagaggtc
    18841 tggaaggtca tccgcgacta caatcgcgtt gcgctgctta gtgcggtcgg gctggtggtc
    18901 gtcagctatc tgatttacgg ctgctatgac ctgctcgccc gcttttactg cggtcacaaa
    18961 ctggcgaagc gccaggtgat gctggtgtcg tttatctgct acgccttcaa cctgacgctc
    19021 agtacctggg tcggcggcat tggtatgcgc tatcgtttgt actcccgact agggttaccg
    19081 ggcagcacta ttacgcggat tttctcgctc agtattacca ccaactggct gggctatatt
    19141 ttactggcag ggattatctt taccgcaggc gtggtggtgt tgccggatca ctggtatgtc
    19201 gctcaaacta cgctgcgtat tctcggcatt ggcttactga tgattatcgc ggtttatttg
    19261 tggttttgcg ctttcgcgaa gcaccgccat atgaccatca aagggcaaaa gctggtgctg
    19321 ccttcatgga aattcgccct cactcagatg ctgatttcca gcgttaactg gatggtaatg
    19381 ggggcgatta tctggctgtt acttggtcaa agcgtgaact atttctttgt actgggcgtg
    19441 ttactggtta gtagtattgc tggcgtcatc gtgcatattc cggcggggat cggtgtgctg
    19501 gaagcggtgt ttatcgcact actggctggg gagcatacct cgaaagggag cattatcgcc
    19561 gcgctgctcg cttatcgcgt cctgtattac tttatcccgc tgctgctggc gctgatctgt
    19621 tacctgctac tggaaagcca ggcgaagaag ctacgggcga aaaatgaagc ggcgatgtga
    19681 aatttagtta agttctttag tatctgcatt cacgactaat gaaaaaaacg cgtatgcctt
    19741 tgccagacaa gcgttatagc tatcgcggca agtatcccaa agaagccgat aaaatagtaa
    19801 tagagtgtca atgcacctgt aaaagcgcaa cgaaccggac tccacaactc ttcactatgg
    19861 cattttcctg ccagttcaag gagcttattt cgtccatgcg agataatgcc tggccataac
    19921 acaatcacta agcaacttat tacccaaatg ggccgttcac tcattaatgc cgcattcacg
    19981 atgattacca gggccaaccc tgtcaacgtc atcataatga taaaacggtg agaaccagga
    20041 tcaacattaa ataataaacc ggccagcata ctgattaaaa acatcgcgcc cgcagtgctg
    20101 aacacaatga taatggcata tacaggaact aaacttgcaa cgacaatcgc caccaaagag
    20161 agtaaaaaca gatatattac gcctgcgatg acggcattta tcttgttgat atccttactt
    20221 aagccataca tacaaactaa taataaccct atggcgacat acattgttgg tgaatcatat
    20281 ttggttgccg taatttcgag atacttagct ccccaggcca gtcctgcggt tgccaccaaa
    20341 cctaaagcgg taatgagcca tataccgttt aatatcctgg tctcgtgctg aaaatctaat
    20401 ttattgctgt tttgtgagta tgattccatt cttttagttg tccttagaaa aaatattaat
    20461 attcaccatc aataaattta aacccacgca tacaaaatac ttttgtattt atggatacgg
    20521 aaaacgcttc cgctcggcca taaggcgtat tttatacctg gtacttgtta agtgtcgttt
    20581 ttagtaagaa aagaaatccc gacacatggc cgggatattg atggtgaaaa agaattaacg
    20641 gcggttgcca aaaattcgca gcaacatcag gaacaggttg atgaagtcca gatacaaggt
    20701 taacgcgcca agaatggaat atttgcgcag gttcgacgtg tcgcgggtat caatctgctc
    20761 acccatattt ttcaatttct gagtgtcata cgccgtcaat ccgacaaaga caatcacgcc
    20821 gatgtaggta actgcccaca tcaatgcttc gcttttcagc cagaagttga ccagcgatgc
    20881 cagcacaatg ccgattaacg ccataaacag catattgcca aagccactta aatcgcgctt
    20941 cgtggtgtaa ccgtacaggc tcattgcgcc gaacatcccg gcagtaacga cgaaagtact
    21001 ggcgatagaa gcagcggtat agacaatgaa tatactggaa agcgtaagac ccgtcagcgc
    21061 cgaataaagc ataaagagca tcgtcgttac acctgcgctc agcttttgaa tcatcgctga
    21121 taacacaata accaatgcta attgcgcgat gatcagaccg attaaaaaga cacggttagt
    21181 gaacaacagc tccatcacgg ccgcggaatt agccgcatac caggcaacaa atgcggtcag
    21241 caacaagcca acggtcatcc agccatagac ttgcgccata taagtttgca agccagcccg
    21301 gggttgtacg attgaatcag aacgtgggaa tctgtccatg acgatctcct gaagatataa
    21361 ggaatatctt aaggatactg caaaatgatg aggctgtgca tcgacgcagc gtaaacgcat
    21421 gtactgagcg gtgaaattgc cggacgcagc ggtgccttat ccggctaaca aaaaactacc
    21481 agcgttttgc cgcctgctga tcgctctccc gagcttcaac ccagcggtcg ccttccggcg
    21541 tggcttcgcg cttccagaac ggtgcgcggg ttttgagata atccataata aactgcccgg
    21601 cttcaaacgc actgctgcga tgcgcactgg tgacaccggc aaaaacgatt tcatcgcccg
    21661 gccataattc cccgatgcgg tgaatcacag tgacgcgccc cagcggccag cggttacgcg
    21721 cttcatcaac aatttctgcc agtgcttttt cagtcattcc cggatagtgt tcgagggtta
    21781 atgctttgac gctgtcgccc aggttatggt tgcgcacctt accagtaaag gtgactaccg
    21841 caccgtcttc gtcacgctcc gccagccacg ggtactcttc tcctacgctg aacggctgcg
    21901 gaccaacaac aattttggtt tctgccatct taacctccgg ttaccggcgg gaagaaagct
    21961 acttcgtcgc cgtcagtcag cggatggtca aaactcacca gcgtctggtt gacggcagcc
    22021 agtaatttgc catcttccag cgccagcgcc cagcgatcgc tctgcgcagc catgtgctgg
    22081 cgtaacgctt caacagttgg gaaatccgca gccacttcgg ttgcatctgt tcccaccaac
    22141 tcgcgcacct gcgcgaaaaa aagaacttta atcatccgct tccaccttaa agtcacccga
    22201 cttgccgccg ctcttcgcca gcaaacgtac cggaccaatc accatatctt tttgcaccgc
    22261 tttgcacatg tcataaatgg tcagcgccgc cacggaggcc gcggttaatg cttccatttc
    22321 gacaccggtt ttcccggtca ggcggcataa ggtttctata cgcacccgat tgtgctccgg
    22381 ctcggcctgt aaattgactt caactttgct gagcatcagc ggatgacaga gcgggatcag
    22441 atcccaggtg cgttttgccg cctgaatacc ggcaatacgc gcagtggcaa atacgtcgcc
    22501 tttgtggtgg cgaccatcaa taatcatcgc cagcgtctcg ctgcgcatgg tgacaaaggc
    22561 ttccgcccgc gcttcacgca cggtttccgc tttggcggag acatccacca tgtgcgcttc
    22621 gccagcggcg ttgatatggg tcagttgcga catacttatt tcttcaaatg tggatggaaa
    22681 ttacacggac gcgtacgggc atccagctgc ggcgcgatga tattttccca tgcggtacgg
    22741 cacgctttgg tcgaacccgg catggcgaaa atcagcgttt tgttggcgac gcccgcaacc
    22801 gcacgagatt gcaacgtgga agtgccaatc tcttcaaacg acaacatccg gaacacttca
    22861 ccaaaacctt caacttcacg gtcgaacaac ggcagcaatg cttcgggagc ctgatcacct
    22921 tccgtcaggc cagtaccacc cgtaatcaat accacttgta catcgtcgct ggcgatccac
    22981 gcagatacct gagcgcgaat agcgtagcgg ttttctttca caatggcttt atcgacaacg
    23041 tgatggcccg cttcttgcgc cgaatcgcgc agatagtgac cggaggtatc gtcttcttca
    23101 ccgcgacgat tagaaaccgt aagaatagca atacgggtcg ggataaattc agtgcttacc
    23161 tgactcatct gatctctcct tttgacgttt tagccgccaa tgtacgataa gttttgcgta
    23221 ataccggtgt tgttttgatg caggaaatgg gtctgtttct tctcccgcag cgccgctgaa
    23281 atacgcgctt ccagcgcctg ttgctgggta tcgtcttcca gcagatcgcg caggttaacg
    23341 ccgccttcac caaacaggca gagatggagt ttaccaatgg aggaaacgcg caggcggttg
    23401 caagtggcgc agaagtcttt ttcatacggc atgataaggc caatctctcc ggcgtaatct
    23461 ggatggcaaa agacttgcgc gggaccgtcg ctgcgttgac gtaattggtg gatccagccg
    23521 cgacgcagta gctcgtcacg cagaacctga ccagagatgt gatgcttacg gaagagctcg
    23581 ctgccctcgc ccgtttccat cagttcgatg aaacgcagct ggatagggcg atgctggatc
    23641 cagttcagaa aggtgtcgag ctggtgatga ttaacatcac gcatcagcac ggtattgact
    23701 ttgaccttct caaaaccggc ctcaaatgca gcatcaatcc ctgccatgac ctggttgaat
    23761 ttatcctgcc cggtaatagc gtgaaactgg cgggcgtcca gactgtcgac actgacgtta
    23821 atgccagtaa gtcccgcatc gcgccagttc gccacatcgc gttccagacg gtaaccattg
    23881 gtggtgaccg caatctggcg gatagcgtcg ttttcccgca cagcggcgat gatatcggta
    23941 aagtcgcggc gtaaagacgg ctcacctcct gtcaggcgca ctttttcggt gcccagactg
    24001 gcgaaggcgc gcgtaacccg gcgaatttca tcgacggtaa gaaagccttt attggtgacg
    24061 ccgctcggtt tgtagccatc cggcaggcag taggtgcaac gaaagttaca cacatcggta
    24121 atcgacaggc gcaagtagta aaacttacgc gcaaatgcat cagtcagttg tgaagccatg
    24181 tacacctttc cagatacggg aggcgaagtc atttcttcct tcgccctggt ggcaatcttt
    24241 acgagcattg tcacggccaa agcaccgtat cagttgaccc aggtgcagag gctagagtgt
    24301 ttagtggtta agccgatact agcgtgtaaa tgagattttt accatccaca ctttcgctat
    24361 gcaatcatgt atatagcgtc atgatcgtgc tatttcgcta cagaataagg gaaaatggca
    24421 gttttacttt gatatacatc atcagggagg catagcgtgc atcgtccttt agaggtagaa
    24481 gaaaatagtt tgtatcctca atattggcag gttaattgct gtttccccgc aatttgcgct
    24541 actgtagcgc ggataatctg actccaggaa actatatgcg caatcgtacg ctggctgatc
    24601 ttgatcgtgt cgttgctctc ggcggagggc atggactggg acgcgttctc tcatcacttt
    24661 cgtctttggg ttctcgttta acgggtatcg tcaccaccac ggataatggt ggctcgacgg
    24721 ggcgtattcg ccgttcagaa ggcggcattg cctggggcga tatgcgcaac tgcctcaacc
    24781 aactgataac ggaaccgagc gtcgcctccg cgatgtttga ataccgtttt ggcggcaatg
    24841 gcgaactttc cgggcacaac cttggaaact tgatgttaaa ggcgctggat caccttagcg
    24901 tgcggcctct ggaagccatc aatttaattc gtaatctgct gaaagtggat acgcatttga
    24961 ttccaatgtc agagcatccc gtcgatctga tggcgattga cgatcagggg catgaagttt
    25021 acggcgaggt caatatcgac cagttaacta cgccgattca agagttatta ttaacgccga
    25081 atgtacccgc aacgcgtgag gcagttcacg ctatcaatga agcggatctc atcattattg
    25141 ggcctggcag tttttatacc agcctgatgc caattctgct gctgaaggaa atcgcccagg
    25201 cattacgccg cacgccagcg ccgatggttt atatcggcaa tctggggcgt gagttgagtt
    25261 tacctgcggc taatttgaag ctggaaagca agctggcaat tatggagcag tatgttggta
    25321 aaaaagtcat tgatgcggtc atcgtcgggc caaaagtgga tgtctcggcg gtgaaagagc
    25381 ggattgtgat ccaggaggta ctggaggcca gcgatattcc gtatcgtcat gaccgccagt
    25441 tgttacataa cgcgctggaa aaggcgttac aggctttagg ttaacggaaa tccgaaggaa
    25501 aattccggct tcctattgaa gacaaagtgc gcgttgttta tgccggatgc ggcgtgaacg
    25561 ccttatccgg cctacaaacc gcgcaaattc aatatattgc ggagaaaatg taggcctgat
    25621 aagcgtagcg catcaggctg ttttccgttt gtcatcagtc ttcttcgcta tcctgttacg
    25681 atgcggcaat aaacaactca cgcaactgat gcaactggtc acgaatttgt gccgcttctt
    25741 cgaactccag attctgcgcg tgttgcatca tcagcccttc cagttcatgg attttctgct
    25801 gcaacgcttt cggcgacata tccatcggca cattatccgg ctcaacaatc gggcgcgatt
    25861 ttcctctgcc cttcgctttg gttttggcaa tgttctgccc cagcgccagg atatcgacca
    25921 ctttcttgtt caagccttgc ggcgtaatgc cgtgttcctc gttgtacttc tgctgtttct
    25981 cacggcgacg ttcggtttcg ccaatcgctt tcgccattga tggggtgatc ttatcgccgt
    26041 agagaatcgc tttaccgtta acgttacgtg ccgcacgacc aatggtctgg atcaacgaac
    26101 gttcggaacg caggaagcct tctttgtcag cgtcgaggat cgccaccagc gacacttccg
    26161 gcatatccag accttcgcgc agtaagttga tccctaccag cacgtcgaac tcacccagac
    26221 gcaagtcgcg gataatctcc atacgttcga cggtgtcgat atctgagtga agataacgca
    26281 cgcgctcgcc atgttcttcg agatattcgg taagatcttc cgccatccgc ttggtcagtg
    26341 tggtgaccag tacgcgttcg ttaattgccg ctcgctgacg aatctccgaa agaagatcat
    26401 caacctgtgt cgccaccggc cgcacttcga taatcgggtc aagcaatccg gttggacgca
    26461 ccacctgatc caccacatcg ccgccggatt tttccagctc gtaattaccc ggcgtcgccg
    26521 aaacatagat ggtttgcggc gctaatgctt cgaactcttc aaacttaagc ggacggttat
    26581 ccagcgctga tggcaggcgg aagccgtact ccaccagtgt ctctttacgc gcccggtcac
    26641 cgcgatacat gccgccaatt tgtggaatgg tgacgtgaga ttcatcgacg accagcagcc
    26701 catcggcagg caggtaatca aacagcgtcg gcggtggctc acccggtcca cgaccggaga
    26761 ggaagcgcga gtagttttca atccccgaac agtagcccag ctcgttcatc atctccagat
    26821 caaactgggt acgctgggtc agccgctgct cttccagcag tttgttgttt tccaacagca
    26881 ctttgcgtct ggcggccagt tcttctttga tctcctccat cgcctgtacg atgcgctcgc
    26941 gcggtgtgac gtagtgcgtt ttcgggtaga tggtaaaacg tggaatagtg gaaacaatct
    27001 gcccggtcag cgggtcaaat aacgacaatc gttccacttc ctcgtcaaac agttccacgc
    27061 gaagtgcaat gtcatccgat tctgccggga agatatcaat cacctcgcca cgaacgcgga
    27121 aagtaccacg ctggaatgct tgatcattac gagcgtattg cagctccgcc agtcggcgca
    27181 gaatcgcgcg ctgatcgata atcataccga ccgtgagatg gagcatcatc ttgagatata
    27241 aatcaggatc gcccagacca taaatcgcgg aaacagaggc cactacaacc acatcacgcc
    27301 gctccagcat cgctttggtg gcggataaac gcatctgctc aatatgttcg ttaaccgagg
    27361 catctttctc aatgaaagtg tcggaactcg gtacataggc ttccggctga tagtagtcgt
    27421 agtaggagac gaaatactcc accgcgtttt ccgggaagaa ctctttcatt tcgccataca
    27481 gctgggccgc cagcgttttg ttgggcgcaa gtaccatggt tgggcgctga aggtcagcaa
    27541 tgacattggc aatggtgaag gttttccctg agccagtcac gccaagtaac gtctggtgcg
    27601 ccaggccatc ttccagcccc tcttcgagac gtcgaatcgc ctctggctga tcgccagaag
    27661 gtttaaaagc ggaattcagt ttgaacggtt tactcatgag tcgctacctg aaggagttgg
    27721 gcgggcaggt atgtaatttt actcgtcgta cttaattatg ccaacaaatt atactggata
    27781 aaaaaacagt tcatcaccat aatatttctg atacagcgta aactccgctg tcaatcatga
    27841 gcaaaattta ctctgtggcg agataaaact ccggccttac cgggttatcc ccaaagcaac
    27901 ggctttttta acatttgtca agatgagtga tgacagtttt atggcaagag atgcctgttc
    27961 agtgactgcc attgcattta tctaaccagt taaaaaataa aagatatttc tttgagccgt
    28021 ctttaacgcc aattcgcgtg caagccgcgt attctctcgc ttgcctcgtg ttttctaact
    28081 ctaatacaca tggttatcca caggaatagt ggataactgt ctccagcccc tatcccccgc
    28141 cgcctgggca attcccacat tcgccgcatg aggcggaagt aaaatttttt tcgtgattat
    28201 tttagaattt aattggttaa attgcagtca atcgaagacg cgatctcgct cgcaatttaa
    28261 ccaaatacag aatggctaca acaaggcaag gtttatgtac tttccggttg ccgcattttc
    28321 tggattttct gcaagccagg ggatctctcc cagcagcggc gcgggaatca tgcgggtgag
    28381 cgtggtcata tattcagcgt gacgttttcc cggaggcgta acatcgttcg ccacccaacc
    28441 cgccagagtc agtccggcgt gttgtattgc ctgcgcagtc aacatcgcgt gattaataca
    28501 gccgagtttc acaccaacta ccagtatcac cggcagttgt tcctgtgtta cccaatctgc
    28561 aaaagtgaaa gtgtcagaaa gcggcgtaaa ccagccgcca gcaccttcca ctaacaccca
    28621 gtcagcctgt tgttcaagcg cgcgtaatcc ggcgctcatt accaatgatt ctatcggcct
    28681 gccctcttgc gcgctgatga tgtgcggcga agtgggttct gcgaaggtgt aaggatttac
    28741 tgttgcgtaa tccagctgca ggctgctgtt gtgctgtaac gccagcgcgt cactattacg
    28801 taagccttcc ggggtctttt cgctgccaga ggcgaccggt ttataacctg ccgtccgata
    28861 acctactgcc ttcgcggctt gtaaaagtgc acaactggcg acggttttcc ccacttcggt
    28921 atccgttccg gtgacaaaat aacgtttact cacgagcaat cactcccaaa aaaagatgat
    28981 acgtcagagg atatcgcccc tgttgttgcg gccaggccag ttgcaatcgc tgcaattgcg
    29041 aacgcgttaa tattcgcggg tcgcgccctt catgaagatg cgtagcaccg atgcctttca
    29101 gcgaacgcat ggcactgagc gcatcatcaa accatagtgt gatgggctga atatgatgtt
    29161 gataatgcac gccgttcagc gactgttcga tttcatctgg cggtaaaaag cgattagcat
    29221 gcggacgctc gtccaccgcc tgccacgcct gatgcagttc gggtaacgat ccctgcacca
    29281 gcgtggtaaa cgccaccacg cctccggggc gcaccacccg atacagctcg cggagtgccg
    29341 tggataaatt accgcaccac tgcaccgcga gattgctcca tgcaagatcg aacgtcgcag
    29401 tcgctaacgg cagggattcg atatctcccg ccagataatg gtctgcagca tctttctggc
    29461 gggcttgcac aagcattggc ggtgagagat ctaacgccgt cacctgtgcg tgacgctctc
    29521 gccagtggcg gctcatccag cctggcccac accccgcgtc cagtacgtgg gtgtatttac
    29581 gctgtgggag cattgccagt aagacgtcag cactccggcg ctgtaactct gcgtgttgct
    29641 catagtgcgc ggctgcccga ccaaatgccg ctgcaatggc ttgtttatta accgttgcca
    29701 tgcagcacct ccagcagacg gtcgatatcc tgcatttcat gcgctgcggt tagcgttaag
    29761 cgcagtcgcg cagtaccagc gggtacggtt ggcgggcgaa tcgccgtgac ccagcagcct
    29821 tgctggcgca gtttttctgc cagttgtaac gcacggctgt tatcaccgac gatcaatggc
    29881 tggatggcgc tgcatgaatc agcaagcgta aacggcaaat cctgtactcc ggcacgaaaa
    29941 cgcgcaatga gtgacaccag tttttcgcgc cgtgcatccc cctcatcacg gcgaatgacc
    30001 gccagcgacg cacgtaatgc ctgcgcctga gcgggcggca tactggtgtt gtagataagg
    30061 tggcgggcga attgcagcag ataatccgcc accgtactgg agcaaagcac cgctgccccg
    30121 ctgacgccaa atcctttgcc aaaagtcacc accagcaatt ctggttttac cttttgcagc
    30181 cagcagctgc cgcgcccctg ctccccgata acgcccgtgc cgtgggcatc atcgaccatc
    30241 aaccagccat tgtgctgttg cgttacctgc tggatttccg ccagtggcgc actatcgccg
    30301 tccatgctga acacgccttc tgtcaccacc agttgctgcc ccggacaggg ggaagcaagc
    30361 agtcgcgcca gatgagtgac atcgttatga acaaaacggc gaagctgcga cgggcttaaa
    30421 ctggcagcct ccagcaatga ggcatggcta agccggtcag cgacaatgcg gtcctctttt
    30481 gccatcatcg cggcaatgac cgcctgatta gcggcaaaac cagagataaa tagcagtgcc
    30541 cgcgaatagc ccagccactc ggccagttct tcttccagcg cctgatgcgc aacgctataa
    30601 ccgctgacgt gaccggaacc gccgctaccg acgccaaatt gctccgcacc ctgctgccag
    30661 gcacggataa tttgcggatg atggcttaaa cctaaataat cgttactgga aaagttcaga
    30721 tactggcgat catccgccac cagccagcgt ccggctcctt gcgccactgg ataacggcga
    30781 cgcagggcat cggcagcacg ccgcgcatcg agcgccgcgt tgattttctc ctgccagctc
    30841 atagtgctgc cgcgttgtaa tattcgtcag tatccggggt catcagcgcc tgttcaagac
    30901 gttgctgttg ttcgttatcc cctgccagca cggcagtttg ctgcggattt agccccagtt
    30961 tgcggaacag ttgcaggtct ttatcttctt ccggattcgg cgtggtcagc agtttgcaac
    31021 cgtagaaaat cgagtttgcg cctgccataa agcacatcgc ctgagtctgt tcgttcatct
    31081 gctcgcgtcc ggcagaaagg cgcacgtaag aggtcggcat catgatccgc gcgaccgcaa
    31141 tggtgcgaat aaaatcaaag gcatcaacgt catcgttatc ggcaagcggc gtgcctttca
    31201 ccttcaccag catgttgatt ggcacgcttt ccggcggcgt cggcaggttt gccagttgca
    31261 gcaataatcc ggcgcgatct tttaccgttt cgcctaagcc cacaatgccg ccagaacaga
    31321 ctttgatccc ggcatcgcgc actttttcca gcgtatcgag gcgttcctga taagtgcggg
    31381 tggtgatgat attgccgtaa aactccggcg aggtgtcgag gttgtggttg tagtaatcca
    31441 gcccggcgtt cgcgaggcgc tgcgcctgag attcactcaa cgtgcccagc gtcatacacg
    31501 cctccagccc taaatctttt accccctgca ccatctgctc caggtacggc atatcgcgtt
    31561 cgttgggatt cttccacgcc gcgcccatgc aaaaacgcgt cgatcccgcc gctttcgctt
    31621 tgcgcgccga ctccagcacc tgttcaactt ccatcaaccg ctcggcttcc agaccggttt
    31681 tatagcgcga gctttgcggg caatatttgc aatcttccgg gcaagcaccg gttttaatcg
    31741 acagcaacgt gctgacctgc acctgacgag gatcgaaatg ctggcgatgc acctgctgcg
    31801 cttcaaacag cagatccagc aacggttttt caaataattc tgcgacttgc gacaatgtcc
    31861 agcgtgggcg gtgagccatg gggcttctcc aaaacgtgtt ttttgttgtt aattcggtgt
    31921 agacttgtaa acctaaatct tttcaatttg gtttacaagt cgattatgac aaaggacgat
    31981 cttgcctttg accaacgcca tatctggcac ccatacacat ccatgacctc cccgctgccg
    32041 gtttatccgg tggtgagcgc cgaaggttgc gagctgattt tgtctgacgg cagacgcctg
    32101 attgacggta tgtcgtcctg gtgggcggcg atccacggct acaatcaccc gcagcttaat
    32161 gcggcgatga agtcgcaaat tgatgccatg tcgcatgtga tgtttggcgg tatcacccat
    32221 gcgccagcca ttgagctgtg ccgcaaactg gtggcgatga cgccgcaacc actggaatgc
    32281 gtttttctcg cggactccgg ttccgtagcg gtggaagtgg cgatgaaaat ggcgttgcag
    32341 tactggcaag ccaaaggcga agtgcgccag cgttttctga ccttccgcaa tggttatcat
    32401 ggcgatacct ttggcgcgat gtcggtgtgc gatccggaca actcaatgca caacctgtgg
    32461 aaaggctatc tgccagaaaa cctgtttgct cccgccccgc aaagccgcat ggatggtgaa
    32521 tgggatgaac gcgatatggt tggctttgcc cgcctgatgg cggcgcatcg tcatgaaatc
    32581 gcggcggtga tcattgaacc gattgtccag ggcgcaggcg ggatgcgcat gtaccatccg
    32641 gaatggttaa aacgaatccg caaaatgtgc gatcgcgaag gtatcttgct gattgccgac
    32701 gagatcgcca ccggatttgg tcgtaccggc aaactgtttg cctgtgaata tgcagaaatc
    32761 gcgccggaca ttttgtgcct cggtaaagcc ttaaccggcg gcacaatgac cctttccgcc
    32821 acactcacca cgcgcgaggt tgcagaaacc atcagtaacg gcgaagccgg ctgctttatg
    32881 cacgggccaa cttttatggg caatccgctg gcctgcgcgg ctgcaaacgc cagcctggcg
    32941 attctcgaat ctggcgactg gcagcaacag gtggcggcta ttgaagtaca gctgcgcgag
    33001 caacttgccc ccgcccgtga tgccgaaatg gttgccgatg tgcgcgtgct gggggcaatc
    33061 ggcgtagtcg aaacaactcg tccggtgaat atggcggcgc tgcaaaaatt ctttgtcgaa
    33121 cagggggtat ggatccggcc ttttggcaaa ctgatttacc tgatgccgcc ctatattatt
    33181 ctcccgcaac agttgcagcg tctgaccgca gcggttaacc gtgcggtgca ggatgaaaca
    33241 tttttttgcc aataacggga agtcagcgtg tgggtttctg gctacacttt ctgcaaacta
    33301 gaaaggaggg ttcatgaaac tcatcagtaa tgatctgcgc gatggcgata agctgccgca
    33361 tcgtcatgtc tttaacggca tgggttacga tggcgataat atttcaccgc atctggcgtg
    33421 ggatgatgtt cctgcgggaa cgaaaagttt tgttgtcacc tgctacgacc cggatgcgcc
    33481 aaccggctcc ggctggtggc actgggtagt tgttaattta cccgctgata cccgcgtatt
    33541 accgcaaggg tttggctctg gtctggtagc aatgccagac ggcgttttgc agacgcgtac
    33601 cgattttggt aaaaccggat acgatggcgc agcgccgccg aaaggcgaaa cccatcgcta
    33661 catttttacc gttcacgcgc tggatgtaga gcatattgat gtcgatgaag gagccagcgg
    33721 cgcgatggtc gggtttaacg ttcatttcca ctctctggca agcgcatcga ttaccgcgat
    33781 gtttagttaa tcactctgcc agatggcgca atgctatctg gtatcactta aaggtattaa
    33841 aaacaacttt ttgtcttttt accttcccgt ttcgctcaag ttagtataaa aaagcaggct
    33901 tcaacggatt catttttcta tttcatagcc cggagcaacc tgtgaacaca ttttcagttt
    33961 cccgtctggc gctggcattg gcttttagcg tgacgctgac cgcctgtagc tcaacaccac
    34021 ccgatcaacg tccttctgat caaactgcgc ctggtacctc ttctcgcccg attctgtcgg
    34081 caaaagaagc gcagaatttc gatgctcaac actattttgc atccctgaca ccaggtgctg
    34141 cagcgtggaa tccttccccg attaccctgc ctgcgcaacc tgactttgtt gtcggcccgg
    34201 cgggcactca aggtgtaacg cataccacga ttcaggcggc ggtagatgcg gcaattatca
    34261 agcgtaccaa caagcgccag tatattgccg tgatgcctgg tgagtatcag ggaacggtgt
    34321 atgtccctgc tgctccgggt ggaattactc tgtacggtac aggtgaaaaa ccgattgatg
    34381 tgaagattgg gctttccctt gatgggggca tgagccctgc cgactggcgt cacgacgtca
    34441 acccgcgcgg caaatatatg ccaggtaaac cagcgtggta tatgtacgat agctgccaga
    34501 gcaaacgcag cgacagtatc ggtgttctct gctctgcggt cttctggtca caaaacaatg
    34561 gcctgcaact gcaaaacctg accatcgaaa acacgctggg cgatagcgta gatgcaggta
    34621 accatccggc ggtggcactg cgtactgatg gtgaccaggt acagattaac aacgttaaca
    34681 ttctcggtcg tcagaacacc ttctttgtca ccaacagcgg tgtgcagaac cgtctggaaa
    34741 cgaatcgtca gccgcgtacg ctggtgacca acagctacat tgaaggggat gtggatatcg
    34801 tttctggtcg cggcgcagtg gtgttcgata acaccgaatt ccgcgtggtg aactcacgta
    34861 ctcagcaaga agcgtatgtg tttgcaccgg ctacgctgtc caacatttac tacggtttcc
    34921 tcgccgtaaa cagccgtttc aatgctttcg gtgatggtgt ggcgcaactg ggccgctcgc
    34981 tggatgttga tgccaatacc aacggtcagg tggtgatccg tgatagcgcc atcaacgaag
    35041 gttttaacac ggctaaaccg tgggccgatg cggtgatctc taatcgtccg tttgcgggta
    35101 ataccggcag cgtagatgat aacgacgaaa tacagcgcaa tctgaatgac actaactaca
    35161 accgcatgtg ggaatacaat aaccgcggcg tgggtagtaa agtggttgca gaggcgaaga
    35221 agtaagagca attaactatt tgccggatgc ggcgtaaacg ccttatccgg cctacggttc
    35281 gatgcgattt gtaggtcgga taagatgcgc aagcatcgca tccgacaata agtgccgaat
    35341 gcgacctaca ttcacatggc gctttttaca tctgacggtt tttattgaag ttaatcaaac
    35401 tacccgcctt gataatctcg cgctcttcag cagtcagact ttccatatag agcgtaattt
    35461 ccgttaccgg cgcatcttca tggatcacat aacctttaaa cgtcgtaccc ggattatcca
    35521 gcgccgcttt aatgccaggg atgtaaatgt aatcccccac ttcaaaggtt ggcacttccg
    35581 ccatctgcag cggtaacatc ccccagttga tgacgttaga acggtagcgt ttagtcgcgt
    35641 actcttcggc aatattcgcc agaccgccaa tcacacgctg gcagctcgcc gcctgttcac
    35701 gcgcagaacc atcgcctggt ttcaccgcat ataccatgct gccaatttca gtttgcagcg
    35761 gatcaatatg ctcctgacca gcaatctgct taatgcgcgc aaacacctct gtcagctcgc
    35821 tgacattccc cgccagacgc tgattttcca gctcagccgt cgctttactt ctgccaacat
    35881 aaccgggatc gcggcgtgac agggtaaact ccgccagacc aatcggattt gaacgataag
    35941 aagaggtttc accggaagga atcagttcgt cggtggtggt cacttcgtcg aggatcttcg
    36001 agcacacttt caggacgata ttgtcagtca gcgcacccaa ttccggccag tctttaatgt
    36061 tcggtccgta aatcagcggt tgctgagttg cccctttcac aaagccctga taaacacggt
    36121 ttttatacgg cgttacatcg aaggcgtact ccggcacgtt gtcccagcaa tcaagttcgc
    36181 tggcagaggt taaatagcca ccgtttgccg cagtcgcagc gatagaacga gcgtccatca
    36241 acgccaccgc tgacatctgc ccattagctg gcttagagcc ttcgcggttc gggaagttac
    36301 gcgtggtgtg gcgaatactt aatccgttgt tgattggcgt atcgcccgcg ccaaagcatg
    36361 ggccgcagaa cgcggttctg atgattgcgc ctgcgccaat caaatctgct accacacctt
    36421 ttttggctaa atccataaac accggctgtg atgacgggta aaccgccagc gagaaggtgt
    36481 cattgccaca ggattgaccg cgcagtgcat tcgccgccgc gatgacgttt tcgtagttac
    36541 cgccagaaca gcccgcgata atcccctgct gcactttcag gcgaccattt tccactttat
    36601 ccagcagcga gagtttggct ttaccgtgcg ccacgcgttc ggactcaatt tcaatctcac
    36661 gcagaatgtc ggtcaggttc tggttcagtg tgtcgatttc atacacgttg ctcgggtgga
    36721 acggcagcgc aatcattggt ttgatggcgc ttaaatcaac gctgatgcag ccatcgtagt
    36781 acgccatcgg ttgagggtta agctggcagt aatcctggcc gcgaccgtgc agcgccagcc
    36841 agttatgcac ttcttcatca gtttgccaga cggagcttaa acaggtggtt tccgtggtca
    36901 tcacgtcaac gctgttacgg aaatcggtag agagcgcgct aacgcccggt ccaacgaact
    36961 ccatgacttt gtttttgacg taaccgtttt tgaacaccgc gccaatgata gccagcgcca
    37021 catcctgtgg ccccacatac ggcgcaggtt ttccggtcag atgcaccgca accacgcccg
    37081 gatagtcgat atcccaggtg tcattaagca gctgttttac caactcaccg ccgccctcac
    37141 cgactgccat tgtccctaat gcaccgtaac gggtgtggct gtctgacccg aggatcattt
    37201 tgcccccgcc tgccatcatc tcacgcatat attgatggat gatcgcaata tgcggaggca
    37261 caaaaatacc gccataacgc tgagctgccg ataaaccaaa aacatggtca tcaccgttaa
    37321 tggtgccgcc gacggcgcag agtgagttat ggcagttggt cagcacatac ggcagcggga
    37381 aacgttccat tccggacgct ttagcggtct gtacaatacc aacaaaggta atatcgtgag
    37441 aggctaatga atcaaactta attttaagtt tatccatatt tccggacgta ttatgagaag
    37501 agagaataga ccaggcaata gtgccttt
//
LOCUS       DAJVOY010000016        37033 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVOY010000016 DAJVOY010000000
VERSION     DAJVOY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.016"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..617)
                     /locus_tag="OQL90_000742"
     CDS             complement(<1..617)
                     /locus_tag="OQL90_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCS1367615.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQ"
     gene            complement(631..1062)
                     /locus_tag="OQL90_000743"
     CDS             complement(631..1062)
                     /locus_tag="OQL90_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367616.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1062..1265)
                     /locus_tag="OQL90_000744"
     CDS             complement(1062..1265)
                     /locus_tag="OQL90_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCS1367617.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1378..2226)
                     /locus_tag="OQL90_000745"
     CDS             complement(1378..2226)
                     /locus_tag="OQL90_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367618.1"
                     /translation="MKKLPPAPFFRLNRAAELLEIDIDDLLIMAAAESIYLSAYLCGY
                     DGTLHSIASLYRNCSLFPDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHV
                     NASYILNIAGLDSIDTPARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYIT
                     RPWVEYIGECMDKNQPIYSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYV
                     KRHYPEECKGKNGKETNEAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERT
                     TAGKKR"
     gene            complement(2322..3581)
                     /locus_tag="OQL90_000746"
     CDS             complement(2322..3581)
                     /locus_tag="OQL90_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1367619.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(3772..4635)
                     /locus_tag="OQL90_000747"
     CDS             complement(3772..4635)
                     /locus_tag="OQL90_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1367620.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4762..5478
                     /gene="rph"
                     /locus_tag="OQL90_000748"
     CDS             4762..5478
                     /gene="rph"
                     /locus_tag="OQL90_000748"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1367621.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            5544..6185
                     /gene="pyrE"
                     /locus_tag="OQL90_000749"
     CDS             5544..6185
                     /gene="pyrE"
                     /locus_tag="OQL90_000749"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1367622.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6222..6818)
                     /gene="slmA"
                     /locus_tag="OQL90_000750"
     CDS             complement(6222..6818)
                     /gene="slmA"
                     /locus_tag="OQL90_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1367623.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6925..7383)
                     /gene="dut"
                     /locus_tag="OQL90_000751"
     CDS             complement(6925..7383)
                     /gene="dut"
                     /locus_tag="OQL90_000751"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1367624.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(7361..8581)
                     /gene="coaBC"
                     /locus_tag="OQL90_000752"
     CDS             complement(7361..8581)
                     /gene="coaBC"
                     /locus_tag="OQL90_000752"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1367625.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8753..9421
                     /gene="radC"
                     /locus_tag="OQL90_000753"
     CDS             8753..9421
                     /gene="radC"
                     /locus_tag="OQL90_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS1367626.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            9638..9874
                     /gene="rpmB"
                     /locus_tag="OQL90_000754"
     CDS             9638..9874
                     /gene="rpmB"
                     /locus_tag="OQL90_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1367627.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            9895..10062
                     /gene="rpmG"
                     /locus_tag="OQL90_000755"
     CDS             9895..10062
                     /gene="rpmG"
                     /locus_tag="OQL90_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1367628.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            10160..10969
                     /gene="mutM"
                     /locus_tag="OQL90_000756"
     CDS             10160..10969
                     /gene="mutM"
                     /locus_tag="OQL90_000756"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1367629.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(11008..11487)
                     /gene="coaD"
                     /locus_tag="OQL90_000757"
     CDS             complement(11008..11487)
                     /gene="coaD"
                     /locus_tag="OQL90_000757"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1367630.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(11495..12772)
                     /gene="waaA"
                     /locus_tag="OQL90_000758"
     CDS             complement(11495..12772)
                     /gene="waaA"
                     /locus_tag="OQL90_000758"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1367631.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            13185..14243
                     /gene="rfaQ"
                     /locus_tag="OQL90_000759"
     CDS             13185..14243
                     /gene="rfaQ"
                     /locus_tag="OQL90_000759"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1367632.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            14240..15364
                     /locus_tag="OQL90_000760"
     CDS             14240..15364
                     /locus_tag="OQL90_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1367633.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            15357..16154
                     /gene="rfaP"
                     /locus_tag="OQL90_000761"
     CDS             15357..16154
                     /gene="rfaP"
                     /locus_tag="OQL90_000761"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1367634.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            16170..17186
                     /gene="waaO"
                     /locus_tag="OQL90_000762"
                     /gene_synonym="rfaI"
     CDS             16170..17186
                     /gene="waaO"
                     /locus_tag="OQL90_000762"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1367635.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            17203..18198
                     /locus_tag="OQL90_000763"
     CDS             17203..18198
                     /locus_tag="OQL90_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCS1367636.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            18208..18900
                     /gene="rfaY"
                     /locus_tag="OQL90_000764"
     CDS             18208..18900
                     /gene="rfaY"
                     /locus_tag="OQL90_000764"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1367637.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            18926..19951
                     /locus_tag="OQL90_000765"
     CDS             18926..19951
                     /locus_tag="OQL90_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCS1367638.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            20036..21019
                     /locus_tag="OQL90_000766"
     CDS             20036..21019
                     /locus_tag="OQL90_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1367639.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRDCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            21065..22318
                     /locus_tag="OQL90_000767"
     CDS             21065..22318
                     /locus_tag="OQL90_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1367640.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(22388..23359)
                     /gene="rfaC"
                     /locus_tag="OQL90_000768"
     CDS             complement(22388..23359)
                     /gene="rfaC"
                     /locus_tag="OQL90_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1367641.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(23363..24409)
                     /gene="rfaF"
                     /locus_tag="OQL90_000769"
     CDS             complement(23363..24409)
                     /gene="rfaF"
                     /locus_tag="OQL90_000769"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1367642.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(24419..25351)
                     /gene="rfaD"
                     /locus_tag="OQL90_000770"
     CDS             complement(24419..25351)
                     /gene="rfaD"
                     /locus_tag="OQL90_000770"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1367643.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            25565..26761
                     /gene="kbl"
                     /locus_tag="OQL90_000771"
     CDS             25565..26761
                     /gene="kbl"
                     /locus_tag="OQL90_000771"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1367644.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26771..27796
                     /gene="tdh"
                     /locus_tag="OQL90_000772"
     CDS             26771..27796
                     /gene="tdh"
                     /locus_tag="OQL90_000772"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1367645.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            28035..29069
                     /gene="waaH"
                     /locus_tag="OQL90_000773"
                     /pseudo
     CDS             28035..29069
                     /gene="waaH"
                     /locus_tag="OQL90_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(29056..30015)
                     /locus_tag="OQL90_000774"
     CDS             complement(29056..30015)
                     /locus_tag="OQL90_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1367646.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(30019..31302)
                     /gene="envC"
                     /locus_tag="OQL90_000775"
     CDS             complement(30019..31302)
                     /gene="envC"
                     /locus_tag="OQL90_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1367647.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(31312..32856)
                     /gene="gpmM"
                     /locus_tag="OQL90_000776"
                     /gene_synonym="pgmI"
     CDS             complement(31312..32856)
                     /gene="gpmM"
                     /locus_tag="OQL90_000776"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1367648.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            33101..33532
                     /locus_tag="OQL90_000777"
     CDS             33101..33532
                     /locus_tag="OQL90_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1367649.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            33674..33925
                     /gene="grxC"
                     /locus_tag="OQL90_000778"
     CDS             33674..33925
                     /gene="grxC"
                     /locus_tag="OQL90_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1367650.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            33988..34455
                     /gene="secB"
                     /locus_tag="OQL90_000779"
     CDS             33988..34455
                     /gene="secB"
                     /locus_tag="OQL90_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1367651.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            34455..35474
                     /gene="gpsA"
                     /locus_tag="OQL90_000780"
     CDS             34455..35474
                     /gene="gpsA"
                     /locus_tag="OQL90_000780"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1367652.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            35554..36375
                     /gene="cysE"
                     /locus_tag="OQL90_000781"
     CDS             35554..36375
                     /gene="cysE"
                     /locus_tag="OQL90_000781"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1367653.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(36428..36901)
                     /gene="trmL"
                     /locus_tag="OQL90_000782"
     CDS             complement(36428..36901)
                     /gene="trmL"
                     /locus_tag="OQL90_000782"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1367654.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         9191 a         8811 c         9220 g         9811 t
ORIGIN      
        1 cactgcttcg ccgtcatgcc acccagcacg atacgggcga tcatgttgct ctcgttgctg
       61 tagtggcgct gctgcgtcgt tttgcccatc tcggcacggg ccatattcag cgcatcgcac
      121 attggcttaa agttgtttgc ggcactgaga cgggctttta gctggcgacg atagcgggcg
      181 gcgatttcag gcacgctacg ctgtaattct tcctcgcact ggatgaaata acggcggata
      241 gcgcggccct gctcgttgcg ttcaaccatt gccacttctt tagccatatc cagcgatagc
      301 aggtaatcat gctctatttg ctggcgagtt tttgcgctcg cccgttttgg cgtgctcaaa
      361 ttttcaacac ggataaaatc ggttccggca ataaaaccgt actggtcaat tcggccttta
      421 atccagttag taaagtcgcg gcccaccccc agcgctttat gcaacgcctt agcgctcaca
      481 atattggctt cacgcccgcc aatgcgtccg gcaataacgg gaacaatagc ggcaaagtcg
      541 ttgccgttaa ttactcccgt gttaacgttg ggttgcgggg cggcccccga actgaatcgg
      601 cttttttcga tagtcatatt ttcggctccg ttatgcggcg gtgagattgt ccgggtatag
      661 attgagaatg tcggcaattt caggctgaga aagtccctga tagccgccag cgtcggcgtt
      721 atggttcaca agccgaataa cttttagaac gtcaccgcgc ccggtaaagc gatagcgtaa
      781 atgtgaccct atcccgtcgg ggttcttttc gtctatacgc tcaagctgaa tatcaagcct
      841 gcgctctaac tcgctggcgt agttccttcc agacgaaagg cggcaataac gcagaatgtc
      901 attttctgtc catccctcta taccagtacg cagcatatag actctggcac gatgcttttt
      961 cggtgtgcgc tttggtgctt gaagggattt tgtaaggggc gtaacatcag atacgcgaat
     1021 atctgaatta gccgcctgta atgggcggtt tttcttttcc atcaggccac cttcccgcgg
     1081 cgttctgcca gccagttgtg aatctcaacc gcatcaaacg cagttatttt ttctcccagc
     1141 tttaccggac gtggtagtga accgtttctt actttacggt cgatggtgga tacactaacg
     1201 cccagcaatt cagccaggcg gaagcgtctg atatagcctt ttgtagggat agtgtcgatg
     1261 ttcatgattc ccgtaacctc ttagcgatag ttgttgcttc gttacgggta tttaatggcg
     1321 tgcacgctca aaaaggaatg atcgtatttt ttactatcgt gatagtaaaa tttactatca
     1381 cctttttttc cctgccgtgg tgcgttcttc cggtgttctg tctgcgtcgg ctatgagttt
     1441 cttcaggtaa tctatggatg gctcatcata gccgcccccc aaaagcgccc agtggtttat
     1501 tgtggcgttc gcccatgctt cgttggtttc cttcccgttc ttccctttac actcttctgg
     1561 gtagtgccgc ttcacataaa tcaacgccaa gaggcaaatt tcgcgctttt gcctgtggcg
     1621 ctctctgttt ccgtgctcaa cgtttttaac atcctcataa agagcttgta agtttatgcg
     1681 agtttcgggg ctgtatatgg gctggttttt gtccatgcat tcgccgatat attcaaccca
     1741 cggccttgtt atgtacaaat cctctacgga tatttcgtgg aaggcatgtt ttgtataatc
     1801 gagaaaaaaa taataattcg cacttacgtc tagttctctt tttctgtgtg cggggctgaa
     1861 tatgcgtgcg ggagtgtcaa tagaatcaag ccctgcgatg ttgagtatat aggaggcgtt
     1921 aacatgccat agcccaagca catggcaatc tatatgattt acaacgcctg ccttaaagct
     1981 tttttcgtcc tctacttcag tgattatgtc ggcaagtaat aagcagtctt ctttgacgtc
     2041 tgggaataaa gagcaattgc ggtaaagact cgcgatgcta tgtaatgtgc cgtcgtagcc
     2101 gcaaaggtac gcgctcaaat atatagattc tgccgccgcc ataatcaaaa gatcatcaat
     2161 atctatttct aataattcgg ctgctctgtt aagcctgaaa aaaggggcgg gtggtaattt
     2221 tttcattgtg tcacctttta cgctgcgcct tattttgtga ttgagccagg cggtaaggct
     2281 tcccgctttt cgccccgtcg agctaggctc aatctattca gttagttcgc ccttctgaga
     2341 ggcgttacgt tgtagtcttt tccgctttcc agtgccacca gcagatcagc ccactgtgcc
     2401 agtgctgctt tacgctcatc aaaatactga tggcggttat aaatcccctc tacgccctta
     2461 atgcgatgat tgaggcatcg ttccgccaca acaggatcaa cacccaacgc tgctaaatga
     2521 gtacgtgcgg tgcgtcgaaa gtcgtgaatc gtgaaattag gcacatccgg catttcggcg
     2581 cgaaccttag ccagtgctac gggtaaagtg ctttcctgaa tatgtgggat cattctgttt
     2641 tgcatcttcc tggccggaag cacccatgcg ctattacatg aaaaggtgtg tagctctctc
     2701 aaccattcaa cggctgggga aggtagaggt atatcaatag ggtctccgtt tttgctgcgt
     2761 tcttccggca aatgccatac cgcaccatct aaatcaaact cttcccatcg tgcggcgcat
     2821 aattccattt tgcggacgca taacgctaac agtaatttga acgtgatttc gttctggcga
     2881 ctgaatccct tagccgtgcg catggctttg aatagctgga ttaactcatc gcgggttaac
     2941 cagcggtcac gggcagcttc tttccctccg gcgtctgcca cctcaaaggc tgaacagggg
     3001 ttaatctcta gcgcgtgccg tttgattccg tagtcgaata tgcggcgcgt ccatctcagc
     3061 acgtccgttg ctatggtcgg cgctccacgg tcaacaatac ctttcagcat gtcatcgata
     3121 tggcgcggtt tcacgtcctc taccttcatg ctgccaatgc aggggtttat atctttgtcg
     3181 atacggcggc ggagtatatc ggggtgcttc cagcgcggga ggatctgacg ctcaaagtat
     3241 tcagcggcaa gctctgaaac gcgcatagcg ttcttctctg cttccatctt cgccagtgct
     3301 tcggttttgc gcttctgctt ctctcctgca acgtcatagc ccagcgcaac gcgagccgat
     3361 agctctttgg ctgtctctct ggcctttgat agtgatagct cgctatacga accaattagc
     3421 atggcgcggg acttccctgc gagtttatag cggaatctcc atgtgggggt tttgtcggct
     3481 tcacggtaac gtaggtataa accgttaccg tctgcccgcc cctcgaatcg ctctccgctc
     3541 ttaatccatg cgcggatctg catgtctgtt agttttggca ttttgcgaat atccaaactt
     3601 tgaatagggg gtgtacccaa agcaatgtac ccaaacctca ttgggtacac ctttgtgtac
     3661 accataatca tgcgctttgt tgcgtcttta tgatagccca tgataacaaa aaagcccgct
     3721 gggttgcggg cttggtgtga tttatgcgtc gtcctaaatg ttgctgatag cttactcgat
     3781 attctgaatc tgctcgcgca tctgctcaat caacactttc agctcaatcg cggagtttgt
     3841 cacttcggca ttgatagact tcgacgcaag agtgttcgac tcgcggttga attcctgcat
     3901 cataaagtcc agacgacgac caacggcttc ttttttcttc agaatgttgt aggtctcttt
     3961 gacatgcgct tccaggcggt ccagttcttc ggcaacgtca attcgttgtg ccagcagaac
     4021 cagctcctgc tccagacggt tgttttccaa ctgaacctga gcatcttcca gcttcgcgac
     4081 cagacgctca cgctgccatt gcaggatttc cggcatatgg gcgcggactt tgaccacttc
     4141 ggcggtgacg ccttccagac gctgctcgat caatgctttc agtgcctgac cttcggtttc
     4201 gcgcgcgaca ataaagtcgt ccagcgtacc atccagcgcc gcgagaattt cagcggcaat
     4261 ggcgtcaaga tcctgctcct gggctgccat cacgcccggc cagcgtagaa tatcaaccgg
     4321 gttgatttcc ccttcgtcac tctgcatttt tacccagttc gcggcagtta ccagctgttt
     4381 agccagtttt tcgttgagga tcagctcacc ttgcgcgcta atatctggct catagcgcag
     4441 ggtacattcc actttaccgc gcgtcaggcg agaacgaata cgctcgcgaa cgacaggttc
     4501 aaggctacgg aactgctccg gcagacgaaa gtaagtttcc agataacgct ggtttaccga
     4561 gcgcatttcc caggttgcgc tcccccattc acccttgatt tcacgccggg cgtaggcggt
     4621 catactgcgg atcatagacg ttcctgttta taaaaggaga ggtggaagga ttatagccat
     4681 cgatgccttg tcaggatagg aataaccgcc ggaagtccgt ataatgcgca gccacatttg
     4741 tttcaagccg gagatttcaa tatgcgtcca gcaggccgta gcaataatca ggtgcgtccc
     4801 gttaccctga ctcgtaacta tacaaaacat gcagaaggct cggtgctggt cgaatttggc
     4861 gataccaaag tgttgtgtac cgcctctatt gaagaaggcg tgccgcgctt cctgaaaggt
     4921 cagggccagg gctggatcac cgcagagtac ggcatgctgc cacgttctac ccacacccgt
     4981 aacgctcgtg aagcggcgaa aggtaagcag ggtggacgca caatggaaat ccagcgtctg
     5041 atcgcccgtg ctcttcgcgc ggcagtagat ttgaaagcgc tgggtgagtt caccattacg
     5101 ctggactgcg acgtgcttca ggctgatggt ggcacgcgta ccgcgtcgat tacgggtgcc
     5161 tgcgtggcgc tggcagatgc gctacagaag ctggtggaaa acggcaagct gaaaaccaac
     5221 ccgatgaaag ggatggtagc cgcagtttct gtcggaattg tgaacggcga agcggtttgc
     5281 gatctggaat acgttgaaga ctctgccgca gagaccgaca tgaacgtagt gatgaccgaa
     5341 gacgggcgca tcattgaagt gcaggggacg gcagaaggcg agccgttcac ccatgaagag
     5401 ctactcacct tgttggctct ggcccgaggg ggaatcgaat ccattgtagc gacgcagaag
     5461 gcggcgctgg caaactgatt tttaaggcga ctgatgagtc gccttttttt tgtctgtaga
     5521 aaagtaagat gaggagcgaa ggcatgaaac catatcagcg ccagtttatt gaatttgcgc
     5581 ttggcaagca ggtgttaaag tttggcgagt ttacgttgaa gtccgggcgc aaaagcccct
     5641 atttcttcaa cgccgggctg tttaataccg ggcgcgatct ggcactgtta ggccgttttt
     5701 acgctgaagc gttggtggat tccggccttg agttcgatct gctgtttggc cctgcttaca
     5761 aagggatccc gattgccacc actaccgccg tggcgctggc ggagcatcat gaccttgacc
     5821 tgccgtactg ctttaaccgc aaagaggcaa aagaccacgg tgaaggcggc aatctggttg
     5881 gtagcgcgtt acaaggacgc gtaatgctgg tagatgatgt gatcaccgcc ggaacggcga
     5941 ttcgcgaatc gatggagatt attcaggcta atggcgcgac gcttgctggc gtgttgattt
     6001 cgcttgatcg tcaggaacgg gggcgcggcg agatttcggc gattcaggaa gttgagcgtg
     6061 attacaactg caaagtgatc tctatcatca ccctgaaaga cctgattgct tacctggaag
     6121 agaagccgga aatggcggaa catctggcgg cggttaaggc ctatcgcgaa gagtttggcg
     6181 tttaaagaaa ctcgccggat gaaaagtcat ccggcgtcat attactgcaa ctgtgccgca
     6241 attagcggcc agcgggcgtc aaaatcatcc gtcgggcggt atttaaattc gctgcggaca
     6301 aaacgtgaca gcataccttc acagaaggcc aggatctggc ttgccagcag ggtttcatcg
     6361 gtggtgtaac cttcaccctc acgcattctc ttttcacgca atacctgacg cagctgcgct
     6421 tcaatacgct cgaacagctg gttgatgcgc ccttgcaggc gatcctgttc aaacattagc
     6481 gcatgaccag tgaggatgcg ggtcaggcca ggattacgct caccaaaacc gagaagcagc
     6541 aacacaatca gacgcaggcg cgctgtggtg tctttctcat ctttcagaat caggttgatg
     6601 cgagtaatca ggctatcttc gataaactca atcaggctat cgaacatgcg ggtcttactg
     6661 gggaagtggc gatacagtgc cgcttcggaa acgccgacag aggcggccag ttttgccgtc
     6721 gtgatacgtt ggcttccatc gctggattcc agcatcagcg ccagagactg aagtatttcc
     6781 tcgcgacggt tccttttcgc agtttgtttt tctgccatgt tacaaaatac ccctgaaaat
     6841 aagcacttgc caggcggcac ccacgctatg accgcaaacg aaatgtttgc ggctatgtta
     6901 tgacgttatt cggatgcgta tgtgttactg acgaccagag tgaccaaagc cgccttcacc
     6961 gcggtcggtg gcgtcgaaat cttccaccag attaaattca gcctgtacta ccggaacaaa
     7021 aatcatctgg gcgatgcgtt cgccaggttg aatggtgaag ctgtcctgac cacggttcca
     7081 cacggaaatc atcaactggc cctgatagtc ggaatcgatc aatcctacca ggttaccaag
     7141 cacgataccg tgcttatgtc ccaatccgga gcgcggcagc atcattgccg ccagtgaagg
     7201 atcggcaata tgaatcgcca gcccggtcgg aaccagcgta gtgtcacccg gagccagttc
     7261 tacggcgtcg tcgagacagg cacgcaggtc aagtccggca gagccagagg tggcataagt
     7321 cgggagcgga aattccttcc caacgcgcgg gtccagaatc ttaacgtcga tttttttcat
     7381 cataacgggt cacgatctcg tcgagtaata gttggccaag gagctctttg cgctcaagcg
     7441 gtaagacttt atctccgtcc tgccagaaaa ggtgtaatgc gttgttttcg ctgttaaatc
     7501 cttgagttgg ctgggaaaca tcgttcgcgc agatcagatc aaggttttta cggatacgtt
     7561 tttgccgggc gtattcttcc acattatttg tttcggcggc aaatccaacg acgtagggtc
     7621 gatggtcttt tagtgcggca acgcctgcga cgatatcggg gtttttaacc atttttattg
     7681 ttaattcatc accctgcgtg gcctgctttt tgattttttc tggggccacg gtagctgcgc
     7741 gataatccgc cacggcggcg cagccgataa aaatattttg ctgctgtaca gaagcattca
     7801 cggcggcttc catttccagc gcggtcatca catcaacacg tttaacaaac ggtggcgtcg
     7861 gtagtgaaac cggacctgat accagcgtga cgttcgcgcc acgacgggca gcggcggcgg
     7921 cgatagcaaa acccatcttg ccggagctgt gattagagat ataacgcacg ggatcgagcg
     7981 gttcacgcgt cggaccggcg gtaatcataa tgttcagatg tttcaggtcg ttgacgggcg
     8041 aaaaatgcgc caccgccata tccacaatgg ttaacggatc gagcattcgc ccaggaccga
     8101 tatcaccaca agcctgactg ccactgtctg gcccccagat gagcaaacca cgggaagcaa
     8161 gcccctctaa attatgctgc gtggcagcgg cacggtacat ctgctggttc atggcgggga
     8221 gcacggctac aggcgcaggt gtagccagac aaatcgtcga taccaggtca ttcgccattc
     8281 cggcagcaac acgggcaatc aaatctgccg tggcaggggc gagaatcact aaatcagccc
     8341 atttacccag ctcaatatgg cccatagcgg cttcggctgc cgggtccagc aaactgtcgg
     8401 aaacgggata accagaaacc gcctgcaagc taagtggggt gataaaggct tttgccgctt
     8461 cggtcatggc tacgcggacg tcggccccgc gatcgcgcaa acgacgcacc agttcagggg
     8521 ttttataggc agcaataccg ccgctaacgc cgagaacgat ttttttaccg gccaggctca
     8581 tgatgatttt tcctgttggg tgacaccaga agttggcgat tttatcacaa tacttttgtt
     8641 gtcgtgcctt cacctgagaa tcactttgcg aggcgctttc caggattgaa aactggccgt
     8701 cgatttaacg gaacggctat gacaggatgc gagcaccaca aaggaggtga aggtgaaaaa
     8761 caattcacag ctgttgatgc cgcgcgaaaa aatgctgaag tttggtatta gcgccttaac
     8821 ggatgtcgag ctgctggcgc tatttctgcg taccggaacg cgcggtaaag atgtattaac
     8881 cctggcaaaa gagatgctgg agaatttcgg ctctctttat ggcttgttaa cctctgaata
     8941 tgaacaattt agtggcgttc atggaattgg cgtggcgaaa tttgcccagt taaaggggat
     9001 tgctgaactg gcgcggcgtt actacaacgt gcggatgcgt gaagaaagcc ctttactcag
     9061 cccggagatg acgcgggaat ttttacaaag ccagcttacg ggtgaggagc gggagatctt
     9121 tatggtgatc tttctcgact cacaacaccg ggttataacg catagccgtc ttttttccgg
     9181 cacgctaaac catgttgaag tccatcctcg ggaaattatc cgcgaagcga taaaaataaa
     9241 cgcctcggcg ctgatccttg cacataatca cccttcgggt tgtgctgaac ccagtaaagc
     9301 ggataaactc attactgaac ggataataaa gagttgtcag ttcatggatt tacgcgtgct
     9361 cgaccatatc gtgattgggc gtggagagta tgtttctttt gccgaacgcg gctggattta
     9421 acccgctatg cgcgatcctt cgggatcttt gtctgttcgg gacttgagca catcgctgag
     9481 tcagcgtata ctacgccacc tttgagaatc tcgggtttgg catttgggcc tggcaatcga
     9541 gagttcacat agaactgcga tgactgggct gtaaagcctg acgaggcgcc aataccccat
     9601 acgaagctcg agctaatttg atttttggag aatagacatg tcccgagtct gccaagttac
     9661 tggcaagcgt ccggtgaccg gtaacaaccg ttcccacgca ctgaacgcga ctaaacgccg
     9721 tttcctgccg aacctgcact ctcaccgttt ctgggttgag agcgagaagc gttttgtcac
     9781 cctgcgcgta tctgctaaag gtatgcgtgt aatcgataaa aaaggcatcg atacagttct
     9841 ggctgaactg cgtgcccgta gcgaaaagta ctaagtactt agaggaaata aatcatggct
     9901 aaaggtattc gtgagaaaat caagctggtt tcttctgctg gtactggtca cttctatacc
     9961 actacgaaga acaaacgtac taagccggaa aaactggaac tgaaaaaatt cgatccagtt
    10021 gttcgccagc acgtgatcta caaagaagcg aaaatcaaat aattctcgct ttgatgtaac
    10081 aaaaaacctc gctccggcgg ggttttttgt tatctgcttg cccccatatt gactgcatct
    10141 gttcattcct ggagatgcta tgcctgaatt acccgaagtt gaaaccagcc gccgcggcat
    10201 agaaccgcat ctcgttggtg caaccattct tcatgcagtg gtgcgcaacg gacgcttgcg
    10261 ctggccggtt tcagaagaga tctaccgttt aagcgaccaa ccagtgctta gcgtgcagcg
    10321 gcgggctaaa tatctgctgc tggagctgcc tgagggctgg attatcattc atttagggat
    10381 gtctggcagc ctgcgcatcc ttccagaaga acttccccct gaaaagcatg accatgtgga
    10441 tttggtgatg agcaacggca aagtgctgcg ctacaccgat ccgcgccgct ttggtgcctg
    10501 gctgtggacc aaagagctgg aagggcataa tgtgctggcc catcttggac cggagccgct
    10561 tagcgacgat ttcaatggtg agtatctgca tcagaagtgc gcgaagaaaa aaacggcgat
    10621 taaaccgtgg ctgatggata acaagctggt ggtaggggta gggaatatct atgccagcga
    10681 atcactgttt gcggcgggga tccatccgga tcggctggcg tcatcactgt cgctggcaga
    10741 gtgtgaattg ttagctcggg tgattaaagc ggtgttgctg cgttcgattg agcagggtgg
    10801 tacaacgctg aaagattttc tgcaaagtga tggtaaaccg ggctatttcg ctcaggaatt
    10861 gcaggtttac gggcgaaaag gtgagccgtg tcgggtgtgc ggtacgccga ttgtggcgac
    10921 taaacatgcg cagcgggcaa cgttttattg tcggcagtgc cagaagtaat tcatgcgcgc
    10981 cggatcgcat accatccggc ataaacgcta cgctaacttc gccatcagcg cctgatggac
    11041 attctccggc aggaaatggg tgacatcgcc ctgatggcgc gccacctctt tcaccaacga
    11101 tgaagagata aacgaccact ctttcgacgg catcagaaac acactttcca gttccggcat
    11161 taagtggcga ttcatatgcg ccagctgcat ttcatattca aaatccgcca ccgcacgtag
    11221 gccacgaatc agcaccgtag cgtgttgatt acgggcgaag ttcgccatca aatcactaaa
    11281 cccaactact tccacgttcc ccagatgcgc ggttgcctgc tgtgccagcg ccacacgctc
    11341 ttccagggta aacatcggtt ttttactggg gctggcggca atcgccagaa taacgtgatc
    11401 gaacatttgc gtggcgcgcg tcacgatatc gatatgacca ttggtaatgg gatcgaaagt
    11461 acccggataa atcgcccgtt tttgcataac aacctcaatg cgttttcggt ggcaggtaag
    11521 gttccagcag ttgaagcagg cgctgtagcg cgccctggtt ttgatacagt acttcaacgg
    11581 catgacggcc atagaaacta cggtaatcgg cgtcggtgag taaagaggaa acctcttttg
    11641 caagcgtagt ggcatcggta acggtaatca gcccgcttgc ctgctccagc cgcgcgcaaa
    11701 tgtctttaaa attaaaagta tgcggcccca tcaataccgg aatagcgtgt gcggcagctt
    11761 ccagcggatt atgcccacca cgttcaacca gtgaaccgcc aacaaaggcg agatcggcaa
    11821 tgccgtacag taacatcaac tcgcccatcg tatcgccaac cacaacctgc gtgctggtgg
    11881 aggggacttc ccctgaagag cgtgtgatat agcttagtcc agcctggcgg acaaggttaa
    11941 tcgcatccgg gaagcgttct ggatgacggg gtaccaggat gagcaataaa ttcgggaatt
    12001 gctgtaacaa tacctgatgt gcggcgatca ccacactctc ttcaccttcg tgagtgctgg
    12061 tggcaatcca taccgggcgg tgtggtgccc actggcggcg cagcgtcacg gctttagcag
    12121 ccaactgcgg cgttacagaa atatcgaatt tcaggctacc ggtaacggtt acctggttat
    12181 tttttgcgcc cagcgccaca aaacgtgcac catcttcttc attttgggcg gcaatcagcg
    12241 taatacgacg cagcaagcga cggacgaatt tacccagttt ggcataacct gcggccgagc
    12301 gggcagagag tcgcgcgttc gcgatcacca gcggaatttt acgtttatgt agcgccgcaa
    12361 tcaggttagg ccatagttcg gtttccataa tcaacaccag tttagggtcg actttattca
    12421 ggaaacgatt aagtgcatcg ggcaggtcat acggcagata aacgtgctga acatccttcc
    12481 cgaaagccga ttgtacgcgc tccgaaccgg ttggcgtcat ggttgtaacg gtaatcggta
    12541 aatcaggata acgatgacgc agcgcgcgca ccaacgggat cgccgccaga gtttcaccga
    12601 cggagacgga gtgcagcata atgccgcctg gttttagcgg atggcggtaa aaaccgtaac
    12661 gttcacccca gcgttttcga taggccggag ccttacgtcc gcgcacccag agccgtatcc
    12721 agatcagcgg ctgaataagg tagagaaggg cggtgtaaag caattcgagc atagtaaata
    12781 gctgacttat ggatgtgctg gggattctat gtatttagct gtggctttac cattactttt
    12841 cccgtttttg acttaaatag caatttaccc gccaattctt gctgaacatc tatacttttt
    12901 ttgtattttt atgcgattca ttgatttctg ctgtacgtgc aggaaggaat cgcagagcaa
    12961 cagtgaaaga atatggttgc taatgatttt atttgattga tttctttatt gaaaatcaac
    13021 aatatgctta aaaatcattc gggtcggatt gccgcgaaag aagtgataca ctagcgcgtc
    13081 aatatcagtg cggtatcagt attcaggtag ctgttgagcc tggggcggta gcgtgctttt
    13141 tctgcttcac cttaatcgga taatctcaac aaaaagagtt acttgtggat aagctatttc
    13201 gaagaatttt gctcattaag atgcgttttc atggggatat gttattaact actcccgtca
    13261 ttagttcgct gaaaaaaaat taccctgacg caaaaatcga tgtgctgctt tatcaggaca
    13321 ccatcccgat cctgtctgaa aatccagaga ttaacgcgct ctacggcata aaaaataaaa
    13381 aagcaaaagc ctcagaaaaa attgccaact tttttcatct catcaaggta ttacgtgcca
    13441 ataagtatga ccttatcgtc aatctcaccg atcaatggat ggttgctata ctggttcgct
    13501 tattaaatgc ccgtgtgaaa atttcccagg attatcatca tcggcagtct gctttttggc
    13561 gtaaaagttt cacccatttg gtgccgttgc agggtggaaa tgtggtggaa agtaacttat
    13621 ccgtgctgac cccattgcga gttgattcgt tggtgaagca gacaaccatg agttacccgc
    13681 ctgcaagctg gaaacgtatg cgtcgcgaac ttgatcatgc tggtgttgga caaaattatg
    13741 tggttatcca acctacggcg cggcaaatct tcaaatgctg ggacaacgcc aagttttccg
    13801 ctgtgattga tgccttacat gctcgtggtt atgaagttgt tctgacgtcc ggcccagata
    13861 aagacgatct ggcctgcgtc aatgaaattg cgcagggatg ccagacgcca ccagtaacgg
    13921 cgctggctgg aaaggtgacc ttcccggaac ttggtgcgtt aatcgatcat gcgcagctgt
    13981 ttattggcgt tgattccgca ccggcgcata ttgccgctgc agttaatacg ccgctgatat
    14041 cgctgtttgg tgcgacagac catattttct ggcgtccctg gtcaaataac atgattcaat
    14101 tctgggcggg agattaccgg gaaatgccaa cgcgcgatca gcgtgaccga aatgagatgt
    14161 atctttcggt tattccggcg gcagatgtca ttgctgctgt cgataaatta ctgccctcct
    14221 ccacgacagg tacgtcgtta tgatcgttgc tttttgttta tataaatatt ttccctttgg
    14281 cggtttgcag cgcgatttta tgcgtattgc tcagacagtc gccgcccgag gtcatcatgt
    14341 tcgggtttat acccagtcgt gggaaggcga ataccctgat gtatttgaac tgatcaaagt
    14401 gccggttaaa tcgcatacca atcacgggcg caatgcggag tattttgcct gggtgcaaaa
    14461 acatttacgc gaacatcccg tcgataaagt cgttggattc aacaaaatgc cggggctgga
    14521 cgtttattat gccgctgatg tttgttatgc cgagaaagtc gcgcaggaaa aaggcttttt
    14581 ctatcacctg acgtcacgtt atcgccatta tgccgccttt gagcgggcaa ccttcgaaca
    14641 gggcaagccg acacagctgc tgatgctgac agataagcaa atcgccgatt tccagaaaca
    14701 ttatcagact gaagcggagc gttttcatat tctgccaccg gggatttatc ctgatcgtaa
    14761 atatagccag cagccagcta atagccgtga aatcttccgt aagaagaatg gaataaccga
    14821 acaacaatat ttattgttgc aggtcggttc agacttcacg cgtaaaggtg tcgatcgttc
    14881 cattgaagca cttgcttcgt taccggattc gctgcgccac aacacattgc tatatgttgt
    14941 tggacaggat aaaccgcgaa aatttgaggc gctggcagaa aaacgcggcg tgcgcagtaa
    15001 tgttcacttc ttctcggggc gcaacgatgt ctcggaatta atggcggcgg cggatttatt
    15061 actgcatcct gcctaccagg aagcagcggg aattgtgcta ctggaagcga ttactgcagg
    15121 attaccggta ctaacaacag ccgtttgtgg ctatgcgcat tatattgtcg acgccaattg
    15181 cggcgaggct attgctgagc cattccgcca ggaaacattg aatgagattt tacgcaaagc
    15241 gttaacgcaa tcttcattgc gccaggcctg ggcggaaaat gcgcgacatt atgctgatac
    15301 acaagattta tacagtctgc cagagaaagc ggcggatatc ataacgggtg gtctggatgg
    15361 ttgaacttaa agagccgttt gccacgttat ggcgcggtaa agatcctttt gaggaagtta
    15421 aaaccttgca gggtgaggtt tttcgtgaac tggaaactcg ccgcactctg cgatttgaaa
    15481 tggcgggcaa aagctatttt ctcaaatggc atcgcggcac gaccctgaaa gagataatca
    15541 aaaatttact ctcattgcgg atgccagtat taggcgcaga ccgcgaatgg agtgcgattc
    15601 atcgactgcg ggatgtcggc gttgatacta tgtatggggt ggcatttggc gaaaaaggta
    15661 ttaatccgct gagcagaacc tcgtttatta ttaccgaaga tctgacccca accatcagtc
    15721 tggaagatta ctgtgctgac tgggcgacca acccaccaga tgttcgcgta aagcgtatgc
    15781 ttattaagcg tgtcgcgacg atggtgcgcg atatgcatgc tgcgggcatt aaccaccgtg
    15841 actgttatat ctgtcatttc ctgctgcact tgcctttttc cggtaaggaa gaggagttaa
    15901 aaatttcggt aattgaccta caccgggcgc agcttcgcac gcgcgttcca cgtcgttggc
    15961 gcgataaaga tcttataggg ctttattttt cttcaatgaa tattggcctg actcagcggg
    16021 atatctggcg gtttatgaaa gtgtattttg ccgccccgct taaagacatt ctcaagcagg
    16081 aacaaggact gctgtcgcaa gcagaagcaa aagccacaaa aatcagggaa agaacgattc
    16141 gaaaatcgtt gtaatttccg gagagaaata tgagtgccca ctattttaat ccacaagaga
    16201 tgatcaataa gacaatcatc ttcgatgaaa ggccagcggc gtcagtggca tcatcattcc
    16261 atgttgctta tggcattgat aaaaactttc tttttggttg tggtgtttca atcacgtcag
    16321 ttttgttaca taacaacgac gtgagttttg ttttccacgt ttttattgat gatatccctg
    16381 aagccgatat ccagcgttta gcccaattgg cgaaaagcta tcgtacctgt atccagatcc
    16441 atctagtaaa ttgtgaacgg cttaaggcat taccgacgac caaaaattgg tctattgcca
    16501 tgtatttccg ttttgtaatt gcagattact ttattgatca acaagataag atcctttacc
    16561 tggatgctga tatcgcctgt cagggaaact taaagccgct gataacaatg gatcttgcca
    16621 ataacgttgc tgctgttgtt actgaacgcg atgctaactg gtggtcgtta cggggtcaaa
    16681 gtctgcagtg taatgaactt gaaaagggct actttaattc aggtgtcctg ttaattaata
    16741 cactagcgtg ggcgcaggag tccgtttctg ctaaagcgat gtcgatgctt gctgataaag
    16801 ccatcgtttc ccgtttcacc tatatggatc aagatatcct taatcttatc ctgttaggga
    16861 aagttaaatt cattgatgct aaatacaata cgcaatttag tttaaattat gaattaaaaa
    16921 aatcatttgt ttgtccaatt aatgatgaaa ccgtattaat tcattatgtc ggcccgacaa
    16981 aaccctggca ttactgggcc ggttatccaa gtgcgcaacc ttttatcaaa gccaaagaag
    17041 catcgccctg gaaaaatgaa ccgttaatgc ggccagttaa ctcaaactat gctcgttatt
    17101 gcgccaagca taattttaaa caaaataaac caattaacgg gataatgaat tatatttatt
    17161 atttttattt aaagataata aaatgaaatt gagaaaagtt aaatgaatga atttataaaa
    17221 gaacggtttt cgtatttagc agataataaa aaagaaaacg ccccagagct aaatgtttcc
    17281 tacggtatcg ataagaattt tttgtatggt gctggcgttt caatttcttc cgttttgatt
    17341 aataattcag atattaattt tgtctttcat gttttcactg attatgtgga tgatgattat
    17401 ttaaagtcat ttaatgaaac agcaaaacaa tttaatacct caattattgt atatttaatt
    17461 gaccccaaat actttgctga tctgccgacg tcacagtttt ggtcatacgc gacatacttc
    17521 agggtattgt cttttgaata tctgagtgaa agtatttcca cactgctgta tctggatgcc
    17581 gatgttgttt gtaaaggaag cctgaaacct ctcacagaaa ttatatttaa agatgagttt
    17641 gctgcggtca ttcctgacaa tgatagtact caggcggcat gtgcaaaacg cctcaacatt
    17701 cccgaaatga atggacgtta tttcaatgca ggcgttatct atgtcaatct taaaaaatgg
    17761 catgaagcaa atttgacacc gtatttactc aaacttttac gaggggaaac taaatatggc
    17821 tctcttaaat atttagatca ggatgcgttg aatatcgcat ttaatatgaa taatatctac
    17881 cttgcgaagg attttgatac tatttatacc ctgaaaaacg aactttatga tcgtagtcat
    17941 cgaaagtatc agcaaaccat taccgataaa acagtgttga ttcactatac agggataact
    18001 aaaccatggc atagctgggc tggatatccg tctgcatcat actttaatat cgcgcgtgaa
    18061 caatctccct ggaagaaata tcctcttaaa gaggcgcgga ctgttgcaga aatgcagaaa
    18121 caatataagc atctgtttgc ccatggtgag tatataaaag gcataacttc attaattaag
    18181 tacaagctta agaaataaat tactcttatg attacaagta tacgttatcg cggcttctca
    18241 ttttattaca aagataatga taataaatat aaagaaatct ttgatgaaat actggcctat
    18301 aattttaaaa cagtaaaagt attatgtaat attgatgata ccaaagtatc gttgattgat
    18361 acgaaatatg gtcgctatgt ttttaaagtc tttgctccga aaacgaaaag gaatgaacgt
    18421 tttttaaaat cttttgtaaa aggtgactac tatcagaatt tgatcgttga aaccgatcgt
    18481 gtcagaagtg caggtcttac ttttcctaat gatttttatt ttctggctga gcgtaaaatt
    18541 ttcaactatg ccagtgtttt tatcatgctt atcgagtatg tcgagggcgt agagctcaat
    18601 gatatgccga ttattccaga aaatgttaaa gcagaaatta aggcttcgat ggaaaaattg
    18661 catgccttaa atatgctatc gggcgaccca caccggggta actttatcgt tagtaaagat
    18721 ggtgtcagga taattgacct atccggtaaa agctgtacgg cagagcgtaa agctcgtgat
    18781 cgtctggcca tggaacgtca cctgggtatt gccaacgaaa taaaagatta tggctactat
    18841 tcagtaatct acagaaccaa actacgcaaa tttattaaaa aattaaaagg caaagcgtaa
    18901 accacacagt caaaacggaa ccaacatgga tttattagca gagagtatta ctgaagtcgc
    18961 tgtctctggg gaaattgcta acaccgatcg tgtgttaaat atcgcttacg gtattgaccg
    19021 taacttttta tttggtgcgg cagtatctat gcaatcagtt gttatgcata acccggacct
    19081 tgcggttaag tttcatctct ttaccgacta cattgatgaa gattatctac aacgtgttaa
    19141 tgcttttacc agcaaaaatg ctaacgttga agtaataatt tataaagtct ccaatgcctt
    19201 tattgatatc ttccccagcc tgaaacagtg gtcttatgca acattcttcc gtttagttgc
    19261 gttccagtat ttgagtgaaa ctattgaaaa tctgttatat atcgatgctg atgtcatctg
    19321 taaaggctca ttagctggat tgcttgatat taattttgat gaggataagt tcgcagctgt
    19381 tattaaagat gtgcctttta tgcaggaaaa accagcgaag cgtctggcta tagagggact
    19441 tccagggaat tatttcaacg ccggtgtagt atatctgcag cttgaagcat gggcgaaaaa
    19501 tgattttatg aataaagcca ttgctatgct ggcaagtgac ccgcagcaca cgaagtataa
    19561 atgccttgat caggatattt taaatattct gttctttggt cattgtaatt ttattagcgg
    19621 cgattatgat tgcttttatg gcattgacta tgagttaaaa aataaaagcg atgaagatta
    19681 taaaaagacc attaccgatg atactaagct gattcattat gttggcgtaa cgaagccctg
    19741 gaacgactgg acgaattatc cctgccagaa gtattttaat gaggcttatc aggcttcttg
    19801 ctggaatgat gtggcgttta ttccagccac gaatgaaaag cagtatcaag tgaaatatca
    19861 acatgcaaag aaaaatggtg atacgtttaa cgcttttatt tacttcatta aatttaaatt
    19921 aaataagtat aaaagaaaac tatttggata ataataaaaa tcatggatgt ctgagttaca
    19981 ataataatta aggatgagta ctatcatatt ttattagttc aggcaagaga acacgatgag
    20041 caatgattac cctttagtat ctataataat accgacgtat aattcatctg attacattac
    20101 tgaaactcta acgaaattag aaaaacaaac ttacccaaat tttgaaattg ttattgttaa
    20161 tgatggttct aaagataaca catcaaacgt tttgagagag tatgggttaa cccactctcg
    20221 attaattatt gtcaataaag aaaatggcgg tgtttcgtct gccaggaata caggtatccg
    20281 caaggcgcaa ggacagttta tatgttttat ggatgatgat gatgagatag atcctaacta
    20341 tctgctgaag atgtatacca gacaacatga gacgggaggg gatgcgattt attgtgggct
    20401 ttatggccat tatataaaag atggtgttac ttactcacct ataaatacag agtttaatga
    20461 aggatcttta cttttcgact ttttttataa aaaggttaga ttccatatag ggtgcttgtt
    20521 tataagaaaa caacttctgg aagagaataa tctttttttt gatgaagatt tacgactagg
    20581 agaagatctt gattttatct atcgactgct aattacatgc gatatgtatg cggttccata
    20641 ttatatgtat aagcataact atagagaaaa ttccttaatg aactcatgta gaaccatcac
    20701 tcattatcga catgagtcat ttgcgcacga aagaatctac tcttctgtga tgcagttata
    20761 caaaggtaac cggaaagaag aaattcatac attattgagt caaaatagag cttatcataa
    20821 aactcgttat ttatggaatg ttctacttaa tggtgatttt aaactattga atcaattagt
    20881 tgaaagcaat gaaaaagaat taagagattg taatcttcct ggcaagagag ataagagacg
    20941 agcaaaaata ttagcatcaa aaaattatat tctctggagg atggtaagac tggtaaatag
    21001 aaaaaagaat aaacgttaga tcttgcatta aataacccat cctcgtagca taggttgaaa
    21061 ttatatgtcg ttttgttgga atgaaattaa ttctggtata aagtctttaa ttcttatatt
    21121 atgtattttt tctttaatga ctttgtcttt atgggatgat gttgcaacaa agtttcttca
    21181 tgcagctgga attatatctg cattgtattt tcttgcgaca ccaaaaaaaa caataactaa
    21241 taatcctact ttgttaattt tcatctcatt atgtcttttg ggtatcgtaa atatcatctg
    21301 gtattcacat tataaagttt caggctctgt ttataccaat gcatatcgtg gcccaatgga
    21361 aactggaaaa attgccttgt gtagcgcctt tattttctta gttctttttg ctaaaaatga
    21421 gatgagaaca aaaataaaat ttgggaaact aattctgttc gcatccctgg caacgcagtt
    21481 actttttttt gcgcatgcca tgtggcaaca tttctattta aacgtcgacc gtgttgcatt
    21541 atcagcttcc cacgctacaa cagcaggcta catcatcctt tttccttctt tactggcatc
    21601 aattctcatt ttaaaatccg actttagaca taaaacaaca ttatatacaa ttaacttcat
    21661 gcttagctta tgtgctgtca tagtaactga gacgcgtgca gccatattag tgtttccatt
    21721 ctttgcgtta atattaatcg taatggatag ttatattaat aagcgaatta attataagtt
    21781 atattgtttt attgcgattg cattattagc aggtgtattt tcttttaaag atacattgct
    21841 tatgagaatg aatgacttaa ataacgattt agttaattat tcgcatgata acaccagaac
    21901 ttcagtcggt gcccgtctgg caatgtatga agttggctta aaaacatatt ctccaatagg
    21961 acaatcactg gaaaaacgtg cagaaaaaat acatgagcta gaagaaaaag agcctagatt
    22021 gagtggcgct ttaccttttg tagattctca tttgcataac gatctcatag atacgttatc
    22081 aacgcgtggt attcctggag ttgtattaac aattttagca ttttcagcaa tactcatata
    22141 tgccttaaga actgctaaag aaccttatat tttaatcttg cttttttcac tactggtagt
    22201 aggcctaagt gatgtaatac tcttttctaa accggttccg actgctgtgt ttgtcaccat
    22261 aatattgctt tgtgcttatt ttaaagcaca atcagaccaa tatttattag ataagtaatg
    22321 aatatatgaa ataaaatcag caaggaggct gattttattt caaaattatc tattaagggg
    22381 ttgtaattta aatcatagca gcgttttctt caatgaatcg cttaacagca tttgctgtca
    22441 attgagacaa ctcattcccc ggagccctac aaaccatttg attcttccca tacccaccaa
    22501 ttaatcccgg atcggttggt ccataaaccg tgatattggg tctatccagt gccgccgtta
    22561 aatggcttaa ccccgtatcc accgacacta caaatttagc cccggccagc acgcgggcaa
    22621 cgccttccag actcatcttc ggcaatactt caacataatc aaatccttcc gccagtcgtt
    22681 tcgcccgttc ttcctcatgc ggcgcgcccc acggaagttt aatccgtatt cctgaatcag
    22741 ccagtaaacc aatcaattct cgccagtgtt cttccggcca gtgtttatca tcacgggtcg
    22801 tcgcatgaag aaatacggca tattcgccag catctgtagg caggttcgtc agaaaatgct
    22861 gtgcgatagc ataatcgccc tgggtttgcg gtttgctata gcccaaactt ttggcaaaca
    22921 gttcgcgggt gcgttctacg gcgtgctgct gttttgcaat atgatgctta caattgtaaa
    22981 acaggctggc taaaggttcg cgagcggttt gccagtccaa tccatgcttt acgccatgcg
    23041 ccagacgtgt caccagtgcc gcgcttttta ccagcccctg agcgtcgata actgcgtcat
    23101 agttctctgc ttgtagcgct tcacgaaacg ctttgcgttc agcttttatg ggggccgaga
    23161 accaggcttt acgccagcga cgtattgcca caggaataac tcgctcaacg gcagcgtgcc
    23221 aggaaggaat ctgtgcgaac ccttcttcca ccacccagtc aaacttaatc cctgggattg
    23281 cctgctgggc atcagtgagt gcgggcaacg tatggagaac atcgcccatc gacgatgttt
    23341 taacgatcaa aacccgcatc cgtcaggctt cctcttgtaa caatagcgtg ttgagttctt
    23401 ccagtacgcg ctggggagta atgtcgatca agctctggtg ataaccctcc gcagcgtcac
    23461 ctttacgcac tttgtgatag ccggtaatca gacggatcac gcgcgcttta tgggatagcg
    23521 gcggtgtgaa gtccgggcta cttggaccat acagggcaac cagcggacga ttgagcgccg
    23581 ccgcaacgtg catcaggcca gaatcgttag tgacaatggc tttacaggct gcaatcagga
    23641 taaccgcttg atcaagctgt gtttcccccg ccaggttccg acaccatgcc tgctgctcgg
    23701 tattcaaagc ggcaagaatc tcattgcccg cttcatgatc tttcgcagag ccaaacagaa
    23761 ccacctgata accttcatca atcagctgct ttgccagttc cgcatagtgg tagtgtggcc
    23821 agcgttttgc cggaccaaac tccgcacccg ggcaaaagcc aatcatcgga cgttctgatg
    23881 aaagcgaaaa ttgattacag gtatatgatt tttcaccttc gctcacctgc aactgcggcc
    23941 ataacaatgg ctgcggcaga tcttgtgcgg tacgcataat gcctttgtca taggccagcg
    24001 cgacatagcg ttccaccatt agcggccagg cttctttatc gagcacgcgt acatcgttga
    24061 gtaaaccgta gcgcatctcg ccgcgccagc cggtgcgatg aggaataccc gcgaagaaag
    24121 gcactaatgc agatttgaag gagttgggta agacgtaggc gcggtcgtag cgcttttcac
    24181 gcaggctatg acccagtttg cggcgttcgc cgatttccag cgctccgtga ccgagaggca
    24241 tagggatagc ttcgttaact tccggcatcc gcgataataa tggacggcac catgccggtg
    24301 ccatcacatc gattatcgcc tggggatagc gcgcctggag cgtgcgatag agactttgcg
    24361 acatcatcat gtcgccaacc caagacgggc cgatcaccag tattttcatg cagagctctt
    24421 atgcgtcgcg attcagccag gccatgtatt ccgttacacc ttcagcaacg gttttgaacg
    24481 gtttgtcgta acccgccgcg cgcagatttg tcagatctgc ctgagtgaac gcctggtagc
    24541 ggcctttcag cttatccggg aacggaatgt attcgatctg gcctttcttg tgataagcca
    24601 gagtagcatc tgctacggcc tggaaggatt ccgcacgacc agtaccgagg ttgaagatac
    24661 cggaaacgcc attttccagg aaccacaggt ttacatctgc cacgtcgccc acatagacga
    24721 agtcacgttt gaagttctcg ctaccttcaa acagcttcgg tgattcaccg ttgttaagct
    24781 gagtgttgag atggaaagcg acgctcgcca tgctgccttt atggccttca cgcggtccat
    24841 aaacgttgaa atagcggaag ccaacaatct gcgagttcgc ttcaggcagg atttggcgaa
    24901 catattcatc aaacaggaat tttgagtaac cgtagacgtt caacggtttt tcgtactcgc
    24961 gggattcaat aaagtcggag gtgcgtccgc cgtaggtggc tgcggaagaa gcgtacagga
    25021 acgggatttc gcgctccagg cagtagtgca gcagctcctt ggagtattga tagttgttat
    25081 ccatcatata cttgccgtcc cactcggtgg tggaagagca tgcgccttcg tggaaaatcg
    25141 cttcgacatc gccgaactct tcgccagcca taatctggat caggaagtct tccttatcca
    25201 tatagtccgc gatatccaga tccaccaggt tcacaaactt ggtgccgtct ttcaggttgt
    25261 ccaccaccag aatatcggtg atgcctttat cattcagggc cttaacgatg ttgctgccga
    25321 taaagcccgc gccgccggta acgatgatca taactgtaac cttcgaatta tggagtcaga
    25381 gacaatctca gacacgaata cttctatcat atcactatac ggcgttggct tcagccattc
    25441 gccaaaagtg agattgatgg acgttattta tcctgaattt tgcagaagtg ttaacgcgtc
    25501 atctcgtcgc gacgtataag tttgggtaat atgtgctgga atttgccctg tctggagaat
    25561 cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat ctggaaaccg cacgggcgga
    25621 agggttgttt aaagaagagc gcattattac gtctgcgcag caagcagata tcactgtggc
    25681 tgatggaagc cacgtcatta acttttgtgc caacaactat ctcgggctgg cgaatcatcc
    25741 ggatctgatt gcggcagcaa aggcgggaat ggattctcac ggtttcggca tggcttcggt
    25801 gcgttttatt tgcggcactc aggacagcca taaagagctt gaacaaaaac tggcggcatt
    25861 cctggggatg gaagatgcga ttctctactc ttcctgcttt gatgctaacg gtggactgtt
    25921 tgaaacgctg ctgggtgcgg aagacgccat tatctccgac gcactgaacc acgcgtctat
    25981 tattgatggt gtgcgtctgt gcaaagctaa acgctatcgc tatgccaaca acgatatgca
    26041 ggagctggaa gcacgtctga aagaagcgcg tgaagccggt gcgcgtcatg tgctgattgc
    26101 caccgatggt gtgttctcaa tggacggcgt gattgccaac ctgaaaggcg tttgcgatct
    26161 ggcagataaa tacgatgcct tggtgatggt ggatgactcc cacgcggtcg gttttgtcgg
    26221 tgaaaatggt cgtggttccc atgaatactg cgatgtgatg ggccgggtcg atattatcac
    26281 cggtacgctt ggtaaagcgc tgggcggggc ttctggtggt tataccgcgg cgcgcaaaga
    26341 ggtggttgag tggctgcgcc agcgttctcg tccgtacctg ttctccaact cgctggcacc
    26401 ggccattgtt gccgcgtcca tcaaagtact ggagatggtc gaagcgggca gcgaactgcg
    26461 tgaccgtctg tgggcgaacg cgcgtcagtt ccgtgagcaa atgtcggcgg cgggctttac
    26521 cctggcggga gccgatcacg ccattattcc ggtcatgctt ggtgatgcgg tagtggcgca
    26581 gaaatttgcc cgagagctgc aaaaagaggg catttacgtc accggtttct tctatccggt
    26641 cgttccgaaa ggtcaggcgc gtattcgtac ccagatgtct gcggcgcata cccctgagca
    26701 aattacgcgt gcagtagaag cattcacgcg tattggtaaa caactgggcg ttatcgcctg
    26761 aggatgtgag atgaaagcgt tatccaaact gaaagcggaa gagggcatct ggatgaccga
    26821 cgtccctgta ccggaactcg ggcataacga tctgctgatt aaaatccgta aaacagccat
    26881 ctgcgggact gacgttcaca tctataactg ggatgagtgg tcgcaaaaaa ccatcccggt
    26941 gccgatggtc gtaggccatg aatatgtcgg tgaagtggta ggtattggtc aggaagtgaa
    27001 aggcttcaaa atcggcgatc gcgtttctgg cgaaggccat atcacctgcg gtcactgtcg
    27061 caactgtcgt ggcggtcgta cccatctgtg ccgcaacacg atcggcgtcg gcgttaaccg
    27121 tccgggctgc ttcgccgaat atctggtgat cccggcgttc aacgccttca aaatcccaga
    27181 caatatctcc gacgacctgg cttccatttt tgatcccttc ggtaacgccg tgcataccgc
    27241 gctgtcgttc gatctggttg gcgaggatgt gctggtctct ggtgcaggtc cgataggtat
    27301 tatggctgcg gcggtggcga aacacgttgg tgcacgcaat gtggtgatca ctgatgttaa
    27361 cgaataccgc cttgagctgg cgcgcaaaat gggtatcacc cgtgcggtta acgtcgcgaa
    27421 agaaaacctt aatgatgtga tggctgaact gggcatgacc gaaggctttg atgtcggtct
    27481 ggaaatgtcc ggtgcgccgc cagcgtttcg taccatgctt gacaccatga accacggcgg
    27541 ccgtattgcg atgctgggta ttccgccgtc tgatatgtct atcgactgga ccaaagtgat
    27601 ctttaaaggc ttgttcatta aaggtattta cggtcgtgag atgtttgaaa cctggtacaa
    27661 gatggcggcg ctgattcagt ctggcctcga tctctcgccg atcattaccc atcgtttctc
    27721 tatcgatgat ttccagaagg gctttgacgc tatgcgttcg ggccagtccg ggaaagtaat
    27781 tctgagctgg gattaacacg aacaagggct ggtattccag cccttttatc tggggataat
    27841 ctgttgaata tgtaaaatcc tgtcagtgta ataaagagtt cgtaattgcg ctgatctctt
    27901 atatagctgt tctcattatc tctctaccct gaagtgactc tctcacctgt aaaaataata
    27961 tctcacaggc ttaatagttt cttaatacaa agcctgtaaa acgtcaggat aacttcagag
    28021 gtcgtcggta atttatgatg aacagcacca ataaacttag tgttattatt ccgttatata
    28081 atgcgggcga tgatttccgc acttgtatgg aatctttaat tacgcaaacc tagactgctc
    28141 tggaaatcat tattattaac gatggttcaa cggataattc tgttgaaata gcaaagcatt
    28201 acgcagaaaa ctatccgcac gttcgtttgt tgcatcaggc gaatgctggc gcatcggtag
    28261 cgcgtaatcg tgggatcgag gtggcgacgg gcaaatatgt cgcttttgtc gatgctgacg
    28321 atgaggtcta tcccaccatg tacgaaacgc tgatgaccat ggcgttagag gacgacctcg
    28381 acgtggcgca gtgcaacgct gactggtctt ttcgtgaaac gggagaaacc tggcaatcca
    28441 tccccagcga tcgccttcgc tcaaccggcg tattaaccgg cccggactgg ctgcggatgg
    28501 ggctttcttc gcgccgttgg actcacgttg tctggatggg ggtttatcgc cgtgatgtta
    28561 ttgttaaaaa taacattaaa tttattgccg gattacatca tcaggatatt gtctggacga
    28621 cagaattcat gtttaacgcg ctgcgtgcgc gatataccga gcaatcatta tataaatatt
    28681 atctgcataa tacgtcagtg agtcggttgc atagacaagg gaataaaaac cttaattatc
    28741 aacgtcacta tattaagatt acccgcctgc tggagaaatt aaatcgaaat tatgccgaca
    28801 aaattacgat ttatccggaa tttcatcagc aaataactta cgaagcattg cgtgtttgcc
    28861 atgcggtgcg caaagagccg gatattctta cccgccaacg gatgattgcc gagatattta
    28921 cttccggtat gtataagcgc ctgattacca atgtgcgcag cgtgaaggtc ggttaccagg
    28981 cgttactgtg gtctttccgc ttatggcaat ggcgcgacaa aacgcggtcg caccatcgca
    29041 ttacgcgtag cgcctttaat ttgcgctagc gttaaacttc gccgcttcgg gctgtttgcc
    29101 ccagccttgc cactgatgct ggaagtaaac gatcagcgtg ctctggctga tgctttcgct
    29161 taatacttca aagaagcgat tagcataaac aggttctatc ggtttcttcg gtttgcacag
    29221 cttcacgcca cggaacggat tacgcggtgc atcaggcaca gcgtttttcg gcggtgtaga
    29281 agtatcaacc tgcggttcat tcagcaagct gctggctttc accagcgtaa tgtctggcgg
    29341 caggttataa accatctgtt gtagcacgcg taccgttaaa gggtgaggat gcccaatggc
    29401 aatggttgaa ccgttgcgac gcgccagatc aattgcgcga ttaaattgca cacggatgtc
    29461 cgcttcgttc tgcgaatcgt cgaggaacac cttccgttta ataaccttca cgccagtacc
    29521 ttgcgcggcg cgcatcgcct gggtattacc gatggttacg ctgtcgagga agtaaagatt
    29581 gtaacgctcc agtgcctgca tcactttctg cataccaaac aggttagagg tcatcttgct
    29641 acccatgtgg ttgttgatcc ccacggcata gggcacgtta ttgaccgcac tacgaataat
    29701 gcgctcaatt tcgtcgctgc tcatctccgg gcgcagcgta tttttctcca gcggctgttt
    29761 actcagtggt gccatcggga gatgaatcaa cacttcgtgc ccgctgttat gcgctttggt
    29821 cgccatctct ctggcgtgcg gtgaatcggg taatacagcg acggagatag cggagggcat
    29881 cgccagcacc tggttttcgt tgtgcgggcg atacccaaaa tcatcaatga cgatggcaag
    29941 tttgccagca agtacggggg aggagagcgc caacagagcg gcaaatgcaa gaacgttacg
    30001 acgaaatgga aacaaaactt atcttcccaa ccacggctgt ggattgaccg cctgaccctg
    30061 gcggcgaatt tcgaaataga gtgaaggccg accctgaccg ccactgctgc ccaccagtgc
    30121 aattggctgg cccgcgcgaa cctgcgaacc aacgctcacc agtgcgctct gattatagcc
    30181 gtaaagactc atgtcgcctt taccgtgctc aaccaccacc accagaccgt aaccttgcag
    30241 ccagtcagcc agaatcactc gaccgtcggc aattgcttta acttcagtac cttcagaggc
    30301 accgataacc atccctttcc agcgtaattc accctgtagc tgctcgccat agcgatgcag
    30361 cgtcggcccg cgaaccggcc agaatgcctg accacgcggc gcacccagac caccagttcg
    30421 ggacatcagc gatttttcgc tttcggtcgg tttgtaggtg gtgcctttgc gcgtcgcttc
    30481 tttctggcgg tcgcgaaccg cctgggcctc acgcgcttct cgttcagcac gcgctttcgc
    30541 cgcggcttcc gcacgggcaa tgctgttacg cagacgggat tcgttggcac gcagttcact
    30601 tagctgctgc tgaccttgct ggatggaaga ttccagcccc gccagcgtct ttttacgctc
    30661 gttcagagcc tgagtcagtt tcgcctgttg ggcgcgctgt tcatataaca gcgtttgttg
    30721 ctcgctttgt ttctcttcca gttcagcacg ctgcatggcg acttcttcac gcgtttgctt
    30781 caactgggca atggtttctt gtcgcgcctg gttgagatag ccgaaataag cctgtaaacg
    30841 ctgtccacac tggctttctt caccgctgag aattagctga ataccggtat gctcgccctg
    30901 acggaatgcg gcatccagtt gtgcggcgag gctgcgctcc tgggcggctt tttgctgctc
    30961 cagtttggca atcgacgcgt tcatctcatc aatctgttta ttgagttgat tgagcgtgtt
    31021 ttgcgtttcg cgcagcttac gggtggcttc agagatcgct tcttcctgct ttttcaattg
    31081 tgcgagcagg cttgcgcgtt gttgttgctt ttggcgtacc gcgcgctctt ttgcggcgat
    31141 atcggcctga atagatttga gttggtcacg ctcatccgcg tgggcggaaa aggcgcacaa
    31201 caatacgcca gcgctaagaa cgctggcgta gatgatgggc ctgattgcaa acctgcgcgg
    31261 tttcacggcc cgtgtcatgg tattaatcgc ctttcccctc atggggaggg attattccac
    31321 gatgaacagc ggcttaccag tcatctcttg cgggatttcc atacccatca gcgacaacat
    31381 ggtcggcgcg atgtcagaaa gtttgccgcc tgcaaccgct ttcacgttct tatcaccaac
    31441 gtaaatcagc ggaactggca ggttggtgtg tgccgtgtgt gcctgacccg ttgccggatc
    31501 gcgcatctgt tcagcgttac cgtggtcagc ggtgatcagc agttgtccac ccacggactc
    31561 aaccgctttc gcgacttctt ccacgcagtg atccagtgct tcaaccgctt taaccgccgc
    31621 ttccatcacc ccggtgtgac ctaccatgtc gccgttcgga tagttacaga taatggtgtc
    31681 gtatttgccg ctcttgatgg ccgcaaccag tttttcggtc agctctgcgg agctcatttc
    31741 cggttgcaga tcgtaggtag ccactttcgg tgagttgatc agaatgcgat cttcgccttt
    31801 gaatgactct tctacgccac cgttgaagaa gaaagtaacg tgggcatatt tttcggtttc
    31861 ggaaatacgc aactgagttt tgtcgttttt cgccatccac tcgccgaagg tgttaaccag
    31921 ggaagcgggt gggtaagcaa ccgcagtttt gatgtcagcg gcgtattcgg tcagcatcac
    31981 gaaatcgacg ttaaccactt tcttacgcgc gaagccgtcg aaatctgcgt ttacgaatgc
    32041 acgagtgatt tcgcgcgcgc ggtcagcacg gaagttcatg aaaatcagcg catcgccgtc
    32101 ttccattgcc gcatctggct gaccttctgc acgaataacg gtcgctttca cgaactcgtc
    32161 gttttcgtcg cgagcataag ctgcctgcaa accagcaacg gcagtatcgg cctggaactc
    32221 accctgcgcc agagtcagca ggtcataggc tttttcaacg cgatcccaac ggttatcgcg
    32281 gtccatcgcg tagtaacgac caatgatgga cgctacgcgg cctttaccca gcgcagcaaa
    32341 tttctcttca aattttttca gcgaggattc agcactgcgc ggcggggtgt cgcgaccgtc
    32401 aaggaatgcg tgcaggtaga ttttttctgc gccgcgttca gctgccagtt ctaccatcgc
    32461 catgatgtga tcttcgtggc tgtgtacgcc gcctgcggag agcagaccca taatgtgtac
    32521 tgctttacct gcgtttttcg ctttatctac cgcgccagtc agcactggat tagcaaagaa
    32581 agcgcgatct ttgatttcaa cgtccagacg agtcaggtcc tgatacacga tgcggccggc
    32641 acccaggtta acgtggccta cttcggagtt acccatctga cggtcaggca gaccgacttc
    32701 cagaccggaa gcgtcgatca gggtatgcgg acgattggcc cacagtgcat ccattaccgg
    32761 ggttttagca ctaaaaatgg cgttatcctg ctgttcttca cgatagccat agccatccag
    32821 aatcaccagt accataggtt ttttagaaac cgacattgcg acaacctcat actcaagagt
    32881 caaaatttgc gtaattttac tacagctgaa tcgataaaat agcctctgaa gatcaaaata
    32941 tacgaccgct cgcggcaagc cgatgccaga tttgcgcttt tttttcgtcg tgccccgcaa
    33001 aattgcattc cagttaacgc gctggctgta tttgccgcac cgcgcaggta tactcctttc
    33061 ctggtttttt taatcactac gtcgggagtt gttacccccc atgcaagaaa ttatgcaatt
    33121 tgttggccgt catcccatac tgagtatcgc ttggatcgcg ttactggtgg cggttcttgt
    33181 gactacgttt aagagcctga cctcgaaagt gaaggtgatt actcgtggtg aagctacgcg
    33241 tctgatcaac aaagaagacg ctgtggttgt ggatttacgt cagcgtgatg acttccgtaa
    33301 aggccatatc gcaggttcta tcaatctgtt gccgagcgaa atcaaagcca acaatgttgg
    33361 tgagcttgag aagcacaaag acaaaccggt tatcgtggta gacggttctg gcatgcagtg
    33421 ccaggagcct gcaaacgcgc tgacgaaagc tggttttgcg caagtattcg tgctgaaaga
    33481 gggcgttgct ggctgggctg gcgaaaactt gcctctggtg cgcggcaaat aatttacctg
    33541 tcgtctttca tgatgcaggc cgttagctgc aattaggccc ccagcgactt attgttataa
    33601 gcttctgggg acactccatt ttgcagccgc cctgcaacat gaaatttcag ggtaaaaaaa
    33661 caggagctaa attatggcca atgttgaaat ctataccaaa gaaacctgcc cgtattgcca
    33721 tcgtgcaaaa gcactgctga gcagcaaggg cgtgagtttt caggagttgc cgatcgatgg
    33781 caatgccgcc aagcgtgaag agatgatcaa acgcagcggt cgcaccacgg ttccccagat
    33841 ttttattgac gcacagcaca ttggcggctg tgatgacttg tatgcattgg atgcacgtgg
    33901 tggactggat cccctgctga aataacgtgt gaacgttggc attacattgc gcagtattta
    33961 aggacaacac ttaagggttt tctacacatg tcagaacaaa acaacactga aatgactttc
    34021 cagatccaac gtatttatac caaggatatc tctttcgaag cgccgaacgc gccgcacgtt
    34081 ttccagaaag attggcaacc agaagttaaa cttgatctgg atacggcatc ttcccaactg
    34141 gcagatgacg tatacgaagt ggtactgcgt gttaccgtaa cggcctccct gggcgaagaa
    34201 accgcgttcc tgtgtgaagt tcagcagggc ggtattttct ccatcgcggg tatcgaaggc
    34261 acccagatgg cacattgcct gggcgcatac tgcccgaaca ttctgttccc gtatgctcgt
    34321 gagtgcatca ccagcatggt atcccgcggt acattcccgc aactgaacct tgcgccggtt
    34381 aacttcgatg cgctgttcat gaactatttg cagcagcagg ctggcgaagg tactgaagaa
    34441 catcaggatg cctgatgaac caacgtaatg cttcaatgac tgtgatcggt gccggctcgt
    34501 acggcaccgc tcttgccatt accctggcaa gaaatggcca cgaggttgtc ctctggggcc
    34561 atgaccctga acatatcgca acgcttgaac gcgaccgctg taacgccgcg tttctccccg
    34621 atgtgccttt tcccgatacg ctccatcttg aaagcgatct cgccactgcg ctggcagcca
    34681 gccgtaatat tctcgtcgtc gtacccagcc atgtctttgg tgaagtgctg cgccagatta
    34741 aaccgctgat gcgtcctgat gcgcgtctgg tgtgggcgac caaagggctg gaagcggaaa
    34801 ccggacgtct gttacaggac gtggcgcgtg aggccttagg cgatcaaatt ccgctggcgg
    34861 ttatctctgg cccaacgttt gcgaaagaac tggcggcagg tttaccgacg gctatttcgt
    34921 tggcctctac cgaccagacc tttgccgatg atctccaaca attgctgcac tgtggcaaaa
    34981 gtttccgcgt ttacagcaac ccggatttca tcggtgtgca gcttggcggt gcggtgaaaa
    35041 acgtcattgc cattggcgcg gggatgtccg acggtatcgg ttttggtgcg aatgcgcgta
    35101 cggcactgat cacccgtggg ctggctgaaa tgtcgcgtct tggcgcggcg ctgggtgccg
    35161 agcctgccac ctttatgggc atggcggggc tgggcgatct tgtgcttacc tgtaccgaca
    35221 accagtcgcg taaccgccgt tttggcatga tgctcggtca gggcatggat gtacaaagcg
    35281 cgcaggagaa gattggtcag gtggtggaag gctaccgcaa tacgaaagaa gtccgcgaac
    35341 tggcgcatcg cttcggcgta gaaatgccaa taaccgagga aatttatcaa gtattatatt
    35401 gcggaaaaaa cgcgcgcgag gcagcattga cgttattagg tcgtgcacgc aaggacgagc
    35461 gcagcagcca ctaaccccag ggaacctttg ttaccgctat gacccggccc gcgcagaacg
    35521 ggccggtcat tatctcatcg tgtggagtaa gtaatgtcgt gtgaagaact ggaaattgtc
    35581 tggaacaata ttaaagccga agccagaacg ctggcggact gtgagccaat gctggccagt
    35641 ttttaccacg cgacgctact caagcacgaa aaccttggta gtgcactgag ctacatgctg
    35701 gcgaacaagc tgtcatcgcc aattatgcct gctattgcta tccgtgaagt ggtggaagaa
    35761 gcctacgccg ctgacccgga aatgatcgcc tctgcggcct gtgatattca ggcggtgcgt
    35821 acccgcgacc cggcagtcga taaatactca accccgttgt tatacctgaa gggttttcat
    35881 gccttgcagg cctatcgcat cggtcactgg ttgtggaatc aggggcgtcg cgcattggca
    35941 atctttctgc aaaaccaggt ttctgtgacg ttccaggtcg atattcaccc ggcagcaaaa
    36001 attggccgtg gcattatgct cgaccacgcc acgggcatcg ttgtgggtga aacggcggta
    36061 attgaaaacg acgtatcgat tctgcaatct gtgacgcttg gcggtacggg taaatctggt
    36121 ggtgaccgtc acccgaaaat tcgtgaaggt gtgatgattg gcgcgggcgc gaaaatcctc
    36181 ggcaatattg aagttggtcg cggcgcgaag attggcgcag gttccgtggt gctgcaaccg
    36241 gtgccgccgc atacaaccgc cgctggcgtt ccggctcgta ttgtcggtaa accagacagc
    36301 gataagccat caatggatat ggaccagcat ttcaacggta ttaaccatac atttgagtat
    36361 ggggatggga tctaatgtcc tgtgatcgtg ccggatgcga tgtaatcatc tatccggcat
    36421 acagtaacta atctctcaac aacgctcccg gatatcccag ctgccgccag gcttcataca
    36481 ccactaccga caccgcattg gacagattca tgctgcggct gtccggcacc atcggaatgc
    36541 gaattttttg ttcagcgggc agggcatcaa gaatgctcgc tggcaggccg cgtgtttccg
    36601 ggccgaacat cagataatcg ccatcctgat agcttacggc gctgtgagca ggcgtacctt
    36661 tcgtggtgag ggcaaacagg cgctggggat tttctgcttc gaggaacgcg cgatagtcat
    36721 gatgacgcgt aacggcggta aactcgtgat agtccagccc cgcgcggcgc aggcgcttat
    36781 cgtcccaggc aaatcccatc ggttcgatga tatgcagacg aaagccggta ttagcgcaaa
    36841 gacggatgat gttgccagta tttggcggaa tttctggttc gtaaagtacg atgtttagca
    36901 tactgccccc ttagtgcggg ggcaggatag cagaaaattg gtgaaccgtg cggccttttc
    36961 ccctcacccc ggccctcccc ctgtctcttg atcaggagat ctgatcaaga gacagctccc
    37021 tgagggagag ggt
//
LOCUS       DAJVOY010000017        36512 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVOY010000017 DAJVOY010000000
VERSION     DAJVOY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.017"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..888
                     /locus_tag="OQL90_000783"
     CDS             <1..888
                     /locus_tag="OQL90_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1367655.1"
                     /translation="FISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEEQIG
                     YTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNTYPT
                     IIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELENLTI
                     SELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINPTI
                     IYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRNH
                     RYTATMLQHIAEDRDGTNH"
     gene            complement(885..2362)
                     /gene="scpC"
                     /locus_tag="OQL90_000784"
                     /pseudo
     CDS             complement(885..2362)
                     /gene="scpC"
                     /locus_tag="OQL90_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(2386..3171)
                     /gene="scpB"
                     /locus_tag="OQL90_000785"
     CDS             complement(2386..3171)
                     /gene="scpB"
                     /locus_tag="OQL90_000785"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1367656.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3182..4177)
                     /gene="meaB"
                     /locus_tag="OQL90_000786"
     CDS             complement(3182..4177)
                     /gene="meaB"
                     /locus_tag="OQL90_000786"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1367657.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKRTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4170..6314)
                     /gene="scpA"
                     /locus_tag="OQL90_000787"
                     /pseudo
     CDS             complement(4170..6314)
                     /gene="scpA"
                     /locus_tag="OQL90_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6518..7411)
                     /gene="argP"
                     /locus_tag="OQL90_000788"
     CDS             complement(6518..7411)
                     /gene="argP"
                     /locus_tag="OQL90_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1367658.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7794..8453
                     /gene="rpiA"
                     /locus_tag="OQL90_000789"
     CDS             7794..8453
                     /gene="rpiA"
                     /locus_tag="OQL90_000789"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1367659.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8708..9940
                     /gene="serA"
                     /locus_tag="OQL90_000790"
     CDS             8708..9940
                     /gene="serA"
                     /locus_tag="OQL90_000790"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1367660.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10169..10228
                     /gene="ibsC"
                     /locus_tag="OQL90_000791"
     CDS             10169..10228
                     /gene="ibsC"
                     /locus_tag="OQL90_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1367661.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10561..10620
                     /locus_tag="OQL90_000792"
     CDS             10561..10620
                     /locus_tag="OQL90_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1367662.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10721..11323)
                     /locus_tag="OQL90_000793"
     CDS             complement(10721..11323)
                     /locus_tag="OQL90_000793"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1367663.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11344..11527)
                     /gene="ssrS"
                     /locus_tag="OQL90_000794"
     ncRNA           complement(11344..11527)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQL90_000794"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11569..11898)
                     /gene="zapA"
                     /locus_tag="OQL90_000795"
     CDS             complement(11569..11898)
                     /gene="zapA"
                     /locus_tag="OQL90_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1367664.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12066..12644
                     /gene="ygfB"
                     /locus_tag="OQL90_000796"
     CDS             12066..12644
                     /gene="ygfB"
                     /locus_tag="OQL90_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1367665.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12670..13995
                     /gene="pepP"
                     /locus_tag="OQL90_000797"
     CDS             12670..13995
                     /gene="pepP"
                     /locus_tag="OQL90_000797"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1367666.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13992..15170
                     /gene="ubiH"
                     /locus_tag="OQL90_000798"
                     /gene_synonym="visB"
     CDS             13992..15170
                     /gene="ubiH"
                     /locus_tag="OQL90_000798"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1367667.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15193..16395
                     /gene="ubiI"
                     /locus_tag="OQL90_000799"
                     /gene_synonym="visC"
     CDS             15193..16395
                     /gene="ubiI"
                     /locus_tag="OQL90_000799"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1367668.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16469..16552)
                     /locus_tag="OQL90_000800"
     CDS             complement(16469..16552)
                     /locus_tag="OQL90_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367669.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16842..17936
                     /gene="gcvT"
                     /locus_tag="OQL90_000801"
     CDS             16842..17936
                     /gene="gcvT"
                     /locus_tag="OQL90_000801"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1367670.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17960..18349
                     /gene="gcvH"
                     /locus_tag="OQL90_000802"
     CDS             17960..18349
                     /gene="gcvH"
                     /locus_tag="OQL90_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1367671.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18468..21341
                     /gene="gcvP"
                     /locus_tag="OQL90_000803"
     CDS             18468..21341
                     /gene="gcvP"
                     /locus_tag="OQL90_000803"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1367672.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYL"
     gene            21607..22350
                     /locus_tag="OQL90_000804"
                     /pseudo
     CDS             21607..22350
                     /locus_tag="OQL90_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22407..23840)
                     /gene="bglA"
                     /locus_tag="OQL90_000805"
     CDS             complement(22407..23840)
                     /gene="bglA"
                     /locus_tag="OQL90_000805"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1367673.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23885..24196
                     /locus_tag="OQL90_000806"
     CDS             23885..24196
                     /locus_tag="OQL90_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCS1367674.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24360..25019
                     /locus_tag="OQL90_000807"
     CDS             24360..25019
                     /locus_tag="OQL90_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1367675.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25215..26195)
                     /gene="ygfZ"
                     /locus_tag="OQL90_000808"
     CDS             complement(25215..26195)
                     /gene="ygfZ"
                     /locus_tag="OQL90_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1367676.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26438..26704
                     /gene="sdhE"
                     /locus_tag="OQL90_000809"
     CDS             26438..26704
                     /gene="sdhE"
                     /locus_tag="OQL90_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1367677.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26685..27092
                     /locus_tag="OQL90_000810"
     CDS             26685..27092
                     /locus_tag="OQL90_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCS1367678.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27132..27653)
                     /gene="fldB"
                     /locus_tag="OQL90_000811"
     CDS             complement(27132..27653)
                     /gene="fldB"
                     /locus_tag="OQL90_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1367679.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27765..28661
                     /gene="xerD"
                     /locus_tag="OQL90_000812"
     CDS             27765..28661
                     /gene="xerD"
                     /locus_tag="OQL90_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1367680.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28686..29396
                     /gene="dsbC"
                     /locus_tag="OQL90_000813"
     CDS             28686..29396
                     /gene="dsbC"
                     /locus_tag="OQL90_000813"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1367681.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29402..31135
                     /gene="recJ"
                     /locus_tag="OQL90_000814"
     CDS             29402..31135
                     /gene="recJ"
                     /locus_tag="OQL90_000814"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1367682.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31226..32324
                     /gene="prfB"
                     /locus_tag="OQL90_000815"
     CDS             join(31226..31300,31302..32324)
                     /gene="prfB"
                     /locus_tag="OQL90_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1367683.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32334..33851
                     /gene="lysS"
                     /locus_tag="OQL90_000816"
     CDS             32334..33851
                     /gene="lysS"
                     /locus_tag="OQL90_000816"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1367684.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33894..34442)
                     /gene="idi"
                     /locus_tag="OQL90_000817"
     CDS             complement(33894..34442)
                     /gene="idi"
                     /locus_tag="OQL90_000817"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1367685.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34497..34568
                     /locus_tag="OQL90_000818"
     CDS             34497..34568
                     /locus_tag="OQL90_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367686.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34565..34690)
                     /locus_tag="OQL90_000819"
     CDS             complement(34565..34690)
                     /locus_tag="OQL90_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367687.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34692..36140)
                     /gene="uacT"
                     /locus_tag="OQL90_000820"
     CDS             complement(34692..36140)
                     /gene="uacT"
                     /locus_tag="OQL90_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1367688.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8793 a         9193 c         9764 g         8762 t
ORIGIN      
        1 tttatttcga aaaagatgcg caatttcatt ttattagcgc aaaccaataa tattgcccgg
       61 gcggcagaaa aaatccatat gacggcttcg ccatttggta aaagcattgc cgcgctggaa
      121 gaacaaattg gctatacgct atttacccgc aaagataata atatcagcct caataaagcc
      181 ggtcaggaac tgtatcaaaa actgttcccg gtttatcaac gactttctgc gattgataat
      241 gaaatccata actcagggcg tcgttcacga gaaattgtga ttggcataga taatacttat
      301 ccgacaatta ttttcgatca gttgattagt ctcggagata agtatgaagg cgtgaccgcc
      361 cagccagttg agttcagcga aaacggcgtt atcgataatc tttttgaccg tcagttagat
      421 tttattatct ccccacagca tgtatctgca cgagttcagg aactggaaaa cctgactatc
      481 agtgaattac caccgctgcg attgggcttt ctcgtttctc gccgttatga agagcgtcaa
      541 gagcaagagc tgcttcagga gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac
      601 ttcgaagcaa tgattgatgc gaatatgcga ccttgtggga tcaatcccac cattatttat
      661 cgtccctaca gttttatggc caaaatcagc gccgtggagc gcggacattt tctgacggtg
      721 atcccgcatt ttgcctggcg actggtgaat ccggcaacgc tgaaatattt tgatgcgccg
      781 cacaggccga tgtatatgca ggaatatctt tattcaatca gaaatcatcg ttataccgcc
      841 acgatgcttc agcatattgc tgaagatcgt gacgggacaa accattaacc cagcatcgag
      901 ccggttgcaa ttaaattacg gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt
      961 ccgccgggcg cattttccag atagcgatgc agataatccc gatacatagg atgtgcacaa
     1021 ttatcaatga tagtgcgggc gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct
     1081 tgttcggtga tgatcacttt gacgctgtgt tcgctgtgat caacatggct gcacattggc
     1141 acgacggttg agatcttgcc ttctttagca atcgacggag ccataaagat cgacagatac
     1201 gcgttgcgtt caaaatcacc gctgccgccg atgccgttca tcagatcgac cccggctacg
     1261 tgcgttgaat ttgcatgccc gtaaatatca aactccaggc cgacgttcag agcgatgacg
     1321 cccagacgac ggatgatttc cgggttattg gaaatctcct gcggacgcaa cacaatgcgg
     1381 ctggcaaagt aatccatatt gtcgtaaatc ttgcgcaggg aatcggccga gattgtcagg
     1441 ctggaggcgc tggccccgct gattttgtcg gtttccagta aatgcaccac cgattcctgt
     1501 agcacttccg aatacatcat gaacggagga atttccgggt tttcccccag acgcgccatt
     1561 accgcattat tgatattgcc cacgccactt tgcagcggca gaaattccgg cggaatacgc
     1621 catgcgccat ttcctgcaat aagaacgtga ccacgttatc ggcaatctgc tggcacatgg
     1681 gattttgctt atccagcata ttaccggcgt cgggcaagtt ggtttccacg acggcgacaa
     1741 tctttttcgg atcgatttgc acatagcggg taccgacgcg atccattgca tggaagatcg
     1801 acacgctatt gcgccgcggt ggcgcgccag gaatcacaat atctgccagt tctgcaacgc
     1861 gcggatcgtg atagtgatta agttcaatga tcactttctt cgctcgcagc agccaggtcg
     1921 gcgcattacc gatcccgctg gttaaccaga ctcgaccatc cggtgccagt gccgatgctt
     1981 caatgacggc aacatcaatg tcgccgaaga aaccgtaatt gaccatttgc gccacttcgc
     2041 tcaaatgcag gtcaacgaaa ctcaccgcgc cctgattgat ctttttacgt aaaccggacg
     2101 atgtttgata tggcgcacgc caggaaacag catcggcgtc agaaagtaca tcgtcagcgg
     2161 cggcgctgat tgacgccccc gtcagcaggc gaatttgata cggctttttg gcctcatgct
     2221 gttcgttagc tctgcgggca atcgcggtgg gtagggcttt cggcgaaccc gccggggtaa
     2281 agccgctaaa tgccaccatg tcgttatgct ggataatttc tgccgcttca tcggcggtca
     2341 tccttgtcca ctgagtttcc atttttactc cttcgttcgc agggattaat gaccaacgaa
     2401 attaggttta cgtttttcga ggaaagcgtt cattccttcc tggtaatctt cgctgtcata
     2461 aaccgcgcgt cgcatcccct gaatacgttc aaattcatcg gagttcatgg tgtgcgcttc
     2521 gcccagtaca cgcagctctt ctttgataac agcaatggct aacggcgctt tctcagagat
     2581 gtggtgcgcc atttgtaagg tgaaatcttc cagttcttcc acttccacaa catggttgag
     2641 gatgccgaca gccagcgcgc gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt
     2701 gacaatgtgg aagcccgcgt cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac
     2761 gccgaggttt acaggcgtca ttgagaaggt tgaggtactg gcggcgatga tcagatcgga
     2821 actcatgatc atttcaaatg cgccacccca aacactacct tccaccatcg aaatgatcgg
     2881 tttcgggaat ttttggatca tgcgggtgat ttgacgcaat ggatcatcat aggagagcgg
     2941 atcgcgaccg ccagacggca gttcgtgaat atcgtgacct gcggagaaga ctttggatcc
     3001 actcggcgcg cgcaaaatga tacagcgaat ttccggccgg ttgagatcgc ttaacgcctg
     3061 cataagatca tcaataaaga ctttacttaa ggcattaagt tttcggccat agttaaactc
     3121 aatgaccgcc actttgttga tagtggcaac gttcacatac tgataagaca taaaaattcc
     3181 tttaatcaaa atattgcgtc tggataaatt cactgagctg ccgcaggccg gtgcgcggtg
     3241 agagcgtatt gtttttgacc gctaaaagcg tctggcggta atagcgatcg aaatcttcat
     3301 tcgcgaacag gtgattcagt acttcttctt cggtctgctt acgcagccat tccaccgatt
     3361 gttgttgccg cacttgttgt aaacgaccac tggcagttag cgcggttttg aactcgatga
     3421 tagcgtgcca gatctcatcg attccacgtt tttccagtgc gctacaagtc agaacccgtg
     3481 gctgccattc gtcgtattta cgtcgcagaa tatgcagggc actctcgtac atatgccggg
     3541 caatggcgac attggtatgg ttatcgccat cgtctttgtt gataacgatc agatcagcca
     3601 cttccatcag ccctttttta atgccctgca gatcatcgcc gccaccggca atttgcaacg
     3661 agataaaaca gtccaccatg cgggcgactt ctgtttccga ctgcccgacg ccaaccgttt
     3721 cgacaatcac tacgtcataa cccgccgctt cgcataacag cattaattcc cgcgctcgct
     3781 gactggcacc gcccagatga ccggaggatg gtaccgggcg aataaacgcc gcttcggcac
     3841 gcgccaggtc attcatgcgg attttatccc cgagaatgct accgccagtg accgggctgc
     3901 tgggatcgac cgcaataacc gcgaccttta atccctctcg aatcaacaac atgccaaagg
     3961 cctcaagaaa ggttcttttc cccgcgctgg gggtgccggt aacgcccagt cgcagggtgt
     4021 taccgcagta cggcataatg gcatcaagca gctgcgtact tagtgcctga tgacgcgggt
     4081 gacggctttc caccagcgtc atggcctggg cgagtgtggc acgctcaccc tgacgtaagc
     4141 ggcgaatact ttctgccagc gtggcttcat taatcatgat gctggcttat cagattcagt
     4201 acgtcgcgca cactgtcgag cataggtgta cctggaccat aaatcgccgc cacgccgcgc
     4261 tcttgcagga aggcgtaatc ctgcggtgga atgacgccac cggcgactac gcagatatct
     4321 tcgcgtcccc attttttcag cgcctcgacc agttccggga tcagcgtttt atgaccggca
     4381 gccagtgagg atgcgcccac tacgtgaacg tcattttcta ctgccaggcg ggcgatctct
     4441 tcaggtgtag agaacatcgg gcttaaatct acgtcgaaac cgagatcgga ataggcgctg
     4501 gcgatcactt tcgagccacg atcgtgccca tcctggccca ttttggcgat cagaatgcgc
     4561 aggcgacgac cattgtcggc aaggaactgc tccgtttgcg caacaatggc atcgaactcg
     4621 gaggccgatt tctcagattg atgatagctt tgcgcaatca cgccggtaac acactggctt
     4681 ggcaccagat aacggtcgaa agcggcttcc agcgcatcgg aaatttcacc cagggtggcg
     4741 cgaacgcgag cggcattaac agcggcagcc agcaggtttt cgttatgctg tgcggcgtga
     4801 gtcagggcgt tcaacgcggc ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc
     4861 agcgaagcaa tttgctcgtt acgcaccatc acgttgtcga tctcaagtac atcggtttcg
     4921 tcttcgtgat ccagtttgta cttgttgaca ccaacgatga cacgtttgcc ctggtcgata
     4981 agcgactgtt cgcgcgctga ggcctattcg atcattcgtt ttggcagacc tgcttcgatc
     5041 gctttcgcca taccaccggc ttcgtcgatc tgttggataa tagctctggc ttgtttgacg
     5101 atttgatcgg tcagcgactc aatgtaatag gatccggcca gtggatcgac ggtgcggcag
     5161 agttctgatt cttcctggat gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg
     5221 gtaggcaaac caagcgcttc gtcaaaggcg ttggtatgca gtgactgagt accgcccagc
     5281 gtcgcagcca gtgcttcaat ggtggtgcgg ataacgttgt tatacggatc ctgttcagtc
     5341 aggctccagc ctgaggtctg gcagtgggta cgcagcgcca gtgatttcgg gtcctgtgcg
     5401 ccaaatccac tgaccgcttc gctccataaa taacgtgccg cacgcaacat ggcgacgttc
     5461 ataaacagat ccatgccgat gccgaagaag aacgacaggc gaggagcgaa gtcatcaatt
     5521 ttcagtccgg cagagattgc tgctttgatg tactcaatcc catcagcgag cgtaaatgct
     5581 acctgctgca cgcagttggc acccgcttca cccatgtggt aaccgctgat actgatggta
     5641 ttaaatcgcg gcatgttgcc ggaacaccag gcgatgatgt cggcgataat gcgcattgac
     5701 ggttttggcg ggtaaatata ggtgttgcgg cagaggtact ctttgaggat atcgttctga
     5761 atggtgccgg tcagtttatc aggtgtaaca ccttgctctt ccgcggcgac gatataaaac
     5821 gccagtactg gtagcactgc gccattcatg gtcatcgaaa ccgacatttt atccagcggg
     5881 atctggtcga acaggacttt catatcttcc acggtgtcga tagcgacgcc cgctttgccg
     5941 acgtcgcccg ccacgcgcgg gttatcggag tcgtagcctc ggtgggtggc aaggtcaaac
     6001 gcaacggaaa gacctttttg cccggcggcc aggttacggc gataaaaagc gttggactct
     6061 tttgctgttg aaaaaccagc atactgacgg atggtccacg gttgggcggt atacatagtg
     6121 gcacgcgggc cacgaacgta gggcggcaaa ccaggaaggg tacctgtcac ctccagatta
     6181 tcgagatcgg cttcggtata cagcggcttg atggtgatcc cttccgcggt ttgctgaacc
     6241 agcgagtcga cagttttctc ccgacggctc aattccttgt tggcaagctg ttgccactcc
     6301 tgcacgttag acattatttg ctccgtaaaa cgcaatcaaa atgagggtat ttgcgcctac
     6361 ggtgagcttt ttacgtgggt ttgtcgccag caaaaaaaat gcattcagac gtcgtttttt
     6421 gccgcctgag atttgtctat tttgtgatgt gattcaagga atacaaaaag aagtgaggac
     6481 aacgcctgat atgtgctatc aggcattatt tgatggatta atcctgacga aggactttgt
     6541 gaccataatc gagcaacgca tcagtgactt tacgcatcat gcggctttca ggagcaaagc
     6601 ggtgccagta gagcatccgg cgttgaaata gccctggcgt taagtcaatc agttcaccgc
     6661 tggctagttc tttctcgatt tgcaggtgcg ggatcataca gcaggtggtg ccctggcgag
     6721 caagttgcac gaacgcttct gaagaattaa cgatatggca gggcacgctg cctggaggca
     6781 gatcgaagtt ttgctgcaaa aaggcctggt gcatatcgtc aagatggtca aaggcgacca
     6841 caggtgcttt caataatgcc gaacgcgtta cgccgttagg gaaatatttt tcggcaaacg
     6901 gttttgagct gacgaacaga tagtcaagcg caccgagctt atcgacaagg caactcggca
     6961 gcgcctgatg ttgaatactc accgcgccga ccacttcgcc gcggcgcaga cgttcctgag
     7021 tacgggtttc atcttctacc tgcaagttga ggcggatagg cgaatcagcc aatacaggag
     7081 ccagtgccgg aagcaaccac gtcgccagac tgtcggcgtt gaccgccagt gaaagcagca
     7141 gcggagtcga accggtttgt tcatcgccca gccactcttc ttccagcaac tccacctggc
     7201 gcagcagtgc cagcagtttt tgcccttgtt ccgtcgggcg cggcggtacg gtacgcacca
     7261 acagcggctg cccgaacata ttttccagtt gcttaatgcg ctgtgagacg gctgattgtg
     7321 taatgcacag cttttgtgcc gcgcgctcaa atcctcgttc acgtatcacc gcatccagtg
     7381 cctgtaatgt tctgtagtcc gggcgtttca ttgctgttgt ctgctcctgg taaatgcttt
     7441 ttcctgcact atgacacaat tttgtgtcag gttgcatata ccgcagccgt cggcgggtaa
     7501 catttgtatc aatggttaaa cgcgcagctt aaaagatcag ggatttgcgc tattgcattt
     7561 cgcttagcga cttcgcgccc tggttatgtt gaatggcgtg gcgttattgc ctctgttcgc
     7621 accgtggagc ggcataccca gaccgttgta tgctttattt gcggggcgaa aggggatgcc
     7681 cgccattgcg cgatatttta tggatgagtt aaccacccgg cttgccaacg gggtctgaat
     7741 cgcttttttt gtatataatg cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc
     7801 aggatgaatt gaaaaaagca gtaggatggg cggcacttca gtatgttcag cccggcacca
     7861 ttgttggtgt aggtacaggt tccaccgccg cacactttat tgacgcgctc ggcacgatga
     7921 aaggccagat tgaaggggcc gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc
     7981 tcggcattca cgtttttgat ctcaacgaag tcgacagcct tggcatctac gttgatggcg
     8041 cagatgaaat caacggccac atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg
     8101 aaaaaatcat tgcttcggtt gcggaaaaat ttatctgtat tgccgacgcc tccaagcagg
     8161 ttgatattct gggtaaattc ccgctgccag tagaagttat cccgatggca cgtagtgcag
     8221 tggcgcgcca gctggtgaaa ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg
     8281 ataatggcaa cgtgatcctc gacgtccacg gcatggaaat ccttgacccg atagcgatgg
     8341 aaaacgccat aaatgcgatt cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg
     8401 cggacgttgc gctgattggc acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg
     8461 ggggaacctc ccccgttaaa aaaattctct tcattaaatt tggtgacatg tgtcacgttt
     8521 ttacctggct attgtcgatt gctctaaata aatcctctaa accagcatat tcaaccaaga
     8581 aatacctttg cgtgatattt cctcaacatc gcgacgcaaa cgttcatatt gccgcaatat
     8641 tattttttga tatgttgaaa ggcggatgca aatccgcaca caacatttca aagacaggat
     8701 tgggtaaatg gcaaaggtat cgctggagaa agacaagatt aagtttctgc tggtagaagg
     8761 cgtgcaccaa aaggcgctgg aaagccttcg tgcagctggt tacaccaaca tcgaatttca
     8821 caaaggcgcg ctggatgatg aacaattaaa agaatccatc cgcgatgccc acttcatcgg
     8881 cctgcgatcc cgtacccatc tgactgaaga cgtgatcaac gccgcagaaa agctggtcgc
     8941 tattggctgt ttctgtatag gaacaaacca ggttgatctg gatgcggcgg caaagcgcgg
     9001 gatcccggta tttaacgcac cgttctcaaa tacgcgctct gttgcggagc tggtgattgg
     9061 cgaactgctg ctgctattgc gcggcgtgcc ggaagccaat gctaaagcgc accgtggcgt
     9121 gtggaacaaa ctggcggcgg gttcttttga agcgcgcggc aaaaagctgg gtatcatcgg
     9181 ctacggtcat attggtacgc aattgggcat tctggctgaa tcgctgggaa tgtatgttta
     9241 cttttatgat attgaaaata aactgccgct gggcaacgcc actcaggtac agcatctttc
     9301 tgacctgctg aatatgagcg atgtggtgag tctgcatgta ccagagaatc cgtccaccaa
     9361 aaatatgatg ggcgcgaaag aaatttcact aatgaagccc ggctcgctgc tgattaatgc
     9421 ttcgcgcggt actgtggtgg atattccggc gctgtgtgat gcgctggcga gcaaacatct
     9481 ggcgggggcg gcaatcgacg tattcccgac ggaaccggcg accaatagcg atccatttac
     9541 ctctccactg tgtgaattcg acaacgtact tctgacgcca cacattggcg gttcgactca
     9601 ggaagcgcag gagaatatcg gcctggaagt tgcgggtaaa ttgatcaagt attctgacaa
     9661 tggctcaacg ctctctgcgg tgaacttccc ggaagtctcg ctgccactgc atggtggtcg
     9721 tcgtctgatg cacatccacg aaaaccgtcc gggcgtgcta actgcgctga acaaaatctt
     9781 cgccgagcag ggcgtcaaca tcgccgcgca atatctacaa acttccgccc agatgggtta
     9841 tgtagttatt gatattgaag ctgacgaaga cgttgccgaa aaagcgctgc aggcaatgaa
     9901 agctattccg ggtaccattc gcgcccgtct gctgtactaa ttccccttct ctgaaaatta
     9961 acgggcaggt cactgacttg cccgtttttt tatcccttct ccacaccgtt gccgcgttgc
    10021 gcattctact ttgcgagtct gctccagaaa ataaatcaaa gtcagatcaa tgcgttgtgt
    10081 ttatttaatg gggatttacg atggcgggac agtatggggc tgtaacggta aagccctcac
    10141 cgaagcgagg gcttaaagga gaagggttat gatgcgactt gtcatcatac tgattgtact
    10201 gttactgata agtttcggag cttattaaca gtcagtctct ggggaggagt catcctccct
    10261 tacccttact cactaaatta ggtcaaagaa tcaacgatgt caatcagggc gatgcggggt
    10321 gcatcgccct gaaccgaact ttttgatagc cacttcatcg cccacgttta ctgccgtaat
    10381 agatcctttt tcccaaaaat tatctctgtt ttgcatgggg tttttcgaac acgctcgaag
    10441 aaaagattta aaggtagatc aatgcgttgt ggttatttaa agagagctta cgatagctgg
    10501 gcagcatggg gctgtaacgg taaagccctc accgaagcga gggcttgaag gagaagggtt
    10561 atgatgcgac ttgccatcat actgattgta ctgttactca taagtttccc agcttattaa
    10621 cagtcagtca caggggagga gcaatcctcc cttaccctta ctcactaaat taggttaaag
    10681 aatcaacgat gtcaatcagg gcgatgcagg tgtatcgccc ttaccactcc cagactttcg
    10741 acggtgtaac caccgcagga agagggatat cccactcttc aacggggagt ttttccacca
    10801 actgacaatc atgcgcataa cccaccggtt gcgttttata gtgctgccag ttttgtaagg
    10861 tccgatcata aaaaccaccg cccattccca ggcgctgacc gtactcatca aaggcgacca
    10921 gcggtgtgat cagcacgtct aatcgggaaa ggggtagcac gtcacgcaca tccagttttg
    10981 gctcatggat tttcaacctg ttcatcacca gttcgctttg cggatggtaa ttcaggaaca
    11041 gcaaattacc agcactaaag ggatgcaaaa ctggaagata tacgcgctta ccggcgcgcc
    11101 agagttgttc tattagcggc tgggtgtcga gttcaccatc aaaagagagg aatacagcga
    11161 ccgtatgcgc catcaccacc gggggataag tcatcatccg ggtagcggct tgttgaccca
    11221 tttcctgctg ttgttccggc gttaacgcac gacgacgttg ccgaatcatt ttgcggattt
    11281 cttgtcggga taatgtcagt gggagttctg gtagttgcgt catggctggt gccagataag
    11341 aagggaatct ccgagatgcc gccgcaggct gtaacccttg aacccttggt tcaaggtgaa
    11401 tgtgtcgtcg cagttttaag gcttctcgga cggaccgagc atgctcacca accgcggagc
    11461 gccacattct tgtggtatga aatatcggct caggggactg gcccgcttgc gaacatctca
    11521 gagaaatttt gtcttcacgg ttactctacc acagtaaacc gaaaagtgtc attcaaagtt
    11581 ttggttagtt ttttcggtga tgcgaccttg ttcaagtaac gcttgttcta tggtctgctg
    11641 cagcatccga atacgctgtt ccatacttgc cgcgtagtca cgagtcttcg ctttttcttg
    11701 cgctaactca tagctgatat tcaatgcggc aatgaagacc aactgttcag tatttgtgac
    11761 tctagtgcgt tctttcagat cttgcaaccg ttggttcaga tcgtccgctg cctgattcaa
    11821 cgcatccctt tggtcaggcg ggcagttcac acgcagtgaa cggccaaaaa tttggatatc
    11881 gacgggttgt gcagacatgc caccttcctg ctgattgact gcgctgcctt cgtctttaga
    11941 cccatggtct gcgaaggggc gacactatag ctaccctgat gagaagagac aagccctttt
    12001 ctggtccacc agggcccaaa gtggtagcat atcatgaata ttcctccctt tgacgacgaa
    12061 tgcttatgtc tatacagaac gaaatgcctg gttacaacga aatgaaccag tatctgaacc
    12121 aacaagggac gggtctgacc cctgccgaga tgcatggttt aatcagtggg atgatatgtg
    12181 gcggtaacga tgacagctca tggctgccgc tacttcacga cctgacgaac gaaggcatgg
    12241 ctttcggtca tgagctggca caggcactgc gtaaaatgca ctctgccacc agcgatgccc
    12301 tgcaggatga cggcttcctt tttcagcttt atctgcctga tggcgatgat gtcagcgttt
    12361 tcgatcgggc tgatgcattg gcaggttggg tcaatcactt cctgcttggt cttggcgtta
    12421 cgcaaccgaa gctggataaa gtgaccggcg aaaccggtga agctatcgac gatctgcgta
    12481 acattgcgca actgggttac gacgaagacg aagatcagga agagcttgaa atgtcgcttg
    12541 aagagatcat cgaatacgtt cgtgttgccg cgctgttatg ccacgacacc tttactcatc
    12601 cgcaaccgac cgcgccagaa gtacaaaaac cgactctaca ctaaaaacaa aaaacgtaag
    12661 gagagtgtta tgagtgagat atcccggcaa gagtttcagc gtcgccgtca ggccctggtg
    12721 gagcaaatgc aacccggcag cgccgcgctg atttttgctg caccagaagt aacacgtagc
    12781 gccgacagcg aataccccta tcgtcagaac agtgacttct ggtacttcac cggctttaac
    12841 gaaccggaag cggtgctggt gctgattaaa agcgatgaca ctcataacca cagcgttctg
    12901 tttaaccgcg ttcgcgacct gacggcggag atctggtttg gccgtcgctt aggccaggat
    12961 gccgcgccag agaaactggg cgttgaccgc gcactggcat tcagcgaaat caatcagcaa
    13021 ctttatcaac tacttaacgg cctggatgtg gtttaccatg cccagggcga atatgcatat
    13081 gctgatgaaa tcatgaacag tgcgctggaa aaactgcgta aaggttcgcg gcaaaatctc
    13141 accgcaccgg caacgatgat cgactggcgt cctgttgttc atgaaatgcg cctgttcaaa
    13201 tcgccagaag agattgccgt actccgccgc gcgggagaaa tcaccgccat ggcacataca
    13261 cgggcgatgg aaaaatgccg tccgggaatg ttcgagtacc atctggaagg cgaaattcac
    13321 cacgaattta accgccacgg tgcgcgctat ccgtcctata acaccattgt cggcagcggt
    13381 gaaaacggct gcattctgca ctacaccgaa aacgagagtg aactgcgcga cggtgacctg
    13441 gtgttgattg acgcgggttg tgaatacaaa ggttacgcgg gcgatattac ccgcaccttc
    13501 ccggtcaacg gcaaattcac ccaggcccag cgtgaaatct acgacattgt gctggagtct
    13561 ctcgaaacca gcctgcgcct gtatcgtccg ggaacttcca ttctggaagt cactggtgaa
    13621 gtggtgcgca tcatggttag cggcctggta aaactcggca ttctgaaagg tgatgttgat
    13681 gaactgatcg ctcagaatgc ccatcgtcct ttctttatgc atggccttag ccactggtta
    13741 gggctagatg tccatgacgt tggcgtttat ggtcaggatc gctcgcgcat tctggaaccg
    13801 ggcatggtac tgaccgtaga gccagggctg tatattgcgc cggatgcaga agtgccagaa
    13861 caatatcgcg gtatcggcat tcgtattgaa gacgacattg tgattaccga aaccggtaac
    13921 gaaaacctca ccgccagcgt ggtgaaaaag ccggaagaaa tcgaagcgtt gatggctgct
    13981 gcgagaaagc aatgagcgta atcatcgtcg gtggcggcat ggcgggcgcg acgctggcgc
    14041 tggctatttc ccggttaagt cacggggcgc tgccggtaca tttgattgaa gcgactgcgc
    14101 cagagtcaca tgctcatccg ggctttgatg gacgagcgat agcgctggcg gcgggtacct
    14161 gtcagcaact ggcgcgcatc ggcgtctggc aatctctggc ggattgcgca actgccatca
    14221 ccaccgtgca tgtcagcgat cgtggtcacg ctggatttgt caccctcgcc gcagaagatt
    14281 accaactggc ggcgctggga caggttgtcg aattgcacaa tgtcgggcaa cggctgtttg
    14341 cattgctgcg taaagcacct ggcgtaacgc tgcattgccc tgatcgcgtg gctaacgttg
    14401 cccgtactca gagtcacgtt gaagtgacgc tggagagtgg cgagacgctg acgggccgcg
    14461 tgctggtagc agctgatggc acccattcag cgttagccac cgcgtgcggc gttgactggc
    14521 agcaggagcc ttacgaacaa ctggccgtga ttgccaacgt tgcgacttcc gttgcgcacg
    14581 aaggccgcgc ttttgaacgc ttcacgcaac atggcccgct ggcgatgttg ccgatgtctg
    14641 acggacgctg ttcgctggtc tggtgtcatc cactggaacg gcgcgaagag gtgctgtcgt
    14701 ggagcgacga gaagttttgc cgtgaactcc agtcggcctt tggctggcga cttggaaaaa
    14761 ttacccacgc cggtaaacgc agtgcttatc cgctggcgtt aacccgcgcc gccagagcta
    14821 ttacccatcg taccgtgctg gtgggcaatg cggcgcaaac tctgcacccg attgccgggc
    14881 aagggtttaa cctcggtatg cgagatgtca tgagccttgc ggaaaccctg actcaggcgc
    14941 atgagcgcgg agaagacatc ggtgattacg gcatattgtg ccgctatcag cagcgtcgac
    15001 agagcgatcg cgaagcaacc atcggcgtca cggacagcct ggtacatctt tttgccaacc
    15061 gttgggcacc gctggttgtc gggcgcaaca tcggactgat gacgatggaa ttattcaccc
    15121 cggcacgcga tgtgctggcg cagcgcaccc tcggttgggt ggcgcgttga aggcggtttt
    15181 aaggaataaa caatgcaatg tgttgatgta gccattgttg gtggcggcat ggtggggctg
    15241 gcggttgcct gcggcttaca ggggagcggc ttacgcgttg ccgtactgga gcagcgcgta
    15301 ccggaacctc tggcggcgga tgcaccacca caactgcgcg tttcggctat caatgccgcc
    15361 agcgaaaaat tactcacccg tcttggcgtc tggcaggaca ttctctctcg tagggccagt
    15421 tgttatcacg gtatggaagt gtgggacaaa gacagttttg gtcacatttc gtttgacgat
    15481 caaagcatgg gctatagcca tcttgggcat atcgttgaaa actcagtgat tcactacgcg
    15541 ctgtggaaca aagcgcagca gtcgtcagat atcactctgt tggcccccgc agaattacag
    15601 caggtcgcct ggggagaaaa tgaaactttc ctgacgctga aagatggcag catgttaacg
    15661 gcgcgtctgg tgattggcgc ggacggtgct aattcctggt tgcgcaacaa agcagatatt
    15721 tcgctgactt tctgggatta tcagcatcac gcgctggtag cgaccattcg cacggaagaa
    15781 ccgcatgatg cggtggcgcg gcaggttttc catggcgaag gcattctggc ctttttaccg
    15841 cttagcgatc cgcatctttg ctcaattgtc tggtcactgt cgccagagga agcgcagcgg
    15901 atgcagcagg caagtgaaga tgaatttaat cgcgcgttaa atatcgcttt tgataatcgc
    15961 ctgggcttat gcaaggttga gagcgcgcgt caggtgttcc cactgacggg gcgttatgcg
    16021 cgccagtttg ccgcgcaccg tctggtgcta gtgggcgacg ccgcgcatac cattcacccg
    16081 ctggcggggc agggagtaaa tctcggcttt atggatgctg cagagctgat tgctgaactg
    16141 aaacggttgc atcgtcaggg gaaagacatc gggcagtaca tttacctgcg ccggtatgag
    16201 cgtagccgca agcacagtgc ggcgctgatg ctggctggta tgcagggatt ccgcgatctg
    16261 ttttccggta ccaatccggc gaaaaaactg ctgcgtgata ttggtttgaa actggccgat
    16321 acgcttcctg gcgttaaacc acaacttatc cgccaggcaa tgggattaaa cgatttgcct
    16381 gaatggctgc gttaaaaatt tctcttctgc tgtttatttg atacccatca cactttcatc
    16441 tcccggtttt ttcgccggga gattttcctc atttgaaata aactaatttc acctccgttt
    16501 tcgcattata ttttctaatg tcattatttt ttgatttagt gttttttgac atttttttag
    16561 ctcttaatat tgccttattc aaattagatt tctaatggca tcatctttat atagaaactg
    16621 gtgtgttttt tggtttttta ttctgtcgcg attttggcgt tttttaacca taagctaatg
    16681 tgatgatcaa ttttacctta tggttaacag tctgtttcgg tggtaagttc aggcaaaaga
    16741 gaacgattgc gttggggacc gggagtggct ccgatgctgg gtttcgtggt gataatttca
    16801 ccatgaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt
    16861 tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc
    16921 cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt
    16981 ttgatgtgtc acatatgacc atcgtcgatc tccgcggcag ccgcacccgg gagtttctgc
    17041 gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag cggcaaagcc ctttactcgg
    17101 ggatgttgaa tgcctctggc ggtgtgatag atgacctcat cgtctactac tttactgaag
    17161 atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc
    17221 aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgattgccg
    17281 tgcaagggcc gaatgcgcag gcaaaagctg ccacactgtt taatgacgcc cagcgtcagg
    17341 cggtggaagg gatgaaaccg ttctttggcg tgcaggcggg cgatctgttt attgccacca
    17401 ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt
    17461 tctggcgtgc gctggtggaa gcgggtgtta agccatgtgg cttgggcgcg cgtgacacgc
    17521 tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt
    17581 tagccgccaa catgggctgg actatcgcct gggaaccggc agatcgtgac tttatcggtc
    17641 gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt ctggtgatga
    17701 ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg cagggcaacc
    17761 agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac agcattgcgc
    17821 tggcgcgcgt gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa
    17881 tgccggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc gcgtgattta
    17941 cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca
    18001 cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg aacatgctca
    18061 ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc
    18121 gggcgatgac tgcgcggttg ccgaatcagt aaaagcggcg tcagacattt atgcgccagt
    18181 aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tctccggaac tggtgaacag
    18241 cgaaccgtat gcaggtggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga
    18301 atcactgctg gatgcgaccg catacgaagc attgttagaa gacgagtaac ggctttattc
    18361 ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt
    18421 aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt
    18481 taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc
    18541 aacagcaaga aatgctgaat gccgttggcg cacaatcgtt aaacgcgctg accggccaga
    18601 ttgtaccgaa agatattcag cttgcgaccc cgccgcaggt tggcgcaccg gcgaccgaat
    18661 acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca
    18721 tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa
    18781 atccgggctg gtataccgcg tacactccgt atcaacctga agtctcccag ggccgccttg
    18841 aagcactgct caacttccag caggtaacgc tggatttgac tggactggat atggcctctg
    18901 cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca
    18961 gcaaactgaa aaatgccaac cgcttcttcg tggcttccga tgtgcatccg caaacgctgg
    19021 atgtggtccg tactcgtgcc gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa
    19081 aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc actaccggtg
    19141 aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagcg
    19201 ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata
    19261 ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg
    19321 cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat
    19381 cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata
    19441 tccgccgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg gcaaacatcg
    19501 ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct aaccgcattc
    19561 accgtttgac cgatatcctg gcggcaggcc tgcaacaaaa aggtctgaag ctgcgccatg
    19621 cgcactattt cgacactctg tgtgtggaag tggccgacaa ggcgggcgta ctggcgcgtg
    19681 ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgcggttggg atcacccttg
    19741 atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag cgtgctgctg ggcgataacc
    19801 acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc tctattcagc
    19861 ctgcgatgct gcgcgacgac gaaatcctca ctcatccggt gtttaatcgc taccacagcg
    19921 aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg
    19981 cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa
    20041 tcacctggcc ggaatttgcc gaactgcacc cgttttgccc gccggaacag gctgaaggtt
    20101 atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg
    20161 tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc
    20221 attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc
    20281 acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg
    20341 ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata
    20401 acctctcctg tatcatggtg acttatccct ctacccacgg cgtgtatgaa gaaacgatcc
    20461 gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca
    20521 tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca
    20581 acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg
    20641 gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca
    20701 tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa
    20761 tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg
    20821 caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata
    20881 ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag
    20941 aaaccagcat cagcgagctg gatattgcca agcgcctgat cgactacggt ttccacgcgc
    21001 cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca
    21061 aagtggaact ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa attgaccagg
    21121 tgaaagccgg tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga
    21181 gcgaactggt cgccgagtgg gcgcatccgt acagccgtga agttgcggta ttcccggcag
    21241 gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta
    21301 acctgttctg ctcctgcgta ccgattagcg aatacctgta attcactgat tcgactattt
    21361 tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg ctcagacagt
    21421 cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac tggcgcgaat
    21481 atttaccctc accccggccc tctccctgaa agggcgaggg gaaaagcgtg ccaacattga
    21541 agattgagcc agtttgttag caatctcaaa gatacgtcaa cgaattaatt tttctcggaa
    21601 aaacaaatgg ctatagcact tgtgaccggt ggcagtcgcg gcatcgggcg ggcaactgca
    21661 ttactgttgg cgaaagaaga gtatacggtg gcggttaatt atcaacaaaa cctccatgcg
    21721 gcgcaggaag tggtgaactt aatcacgcaa gccggtggca aggcattcgt gctccaggcg
    21781 gatatcagcg acgaaaatca ggtcatcgca atgtttacag caatcgatca gcacgatgaa
    21841 ccgctagcag cgctggtcaa taacgccggg atcttgttta cccagtgcac cgttgaaaac
    21901 cttaccgcag agcgaatcaa ccgagtactt tccaccaacg tgacgggata ttttctctgc
    21961 tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg cgctatcgtc
    22021 aatgtctctt cggtggcctc acggttgggt tcgccagggg aataggttga ttacgcggca
    22081 tcgaaagggg cgattgatac gttaaccacc ggactatcgc tggaagtcgc cgcgcagggg
    22141 atccgcgtta actgcgtgcg gccagggttt atttataccg aaatgcacgc cagcggcggc
    22201 gagcttggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg tggacaggca
    22261 gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta cgtcacggga
    22321 agttttatca atttggcggg cgggaaataa aacagggaag ttgtctgacc ggatgcaaca
    22381 agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc aatcacctct
    22441 ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc gtcgtcatgt
    22501 ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa tgacacgcag
    22561 tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt cactgctttt
    22621 ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt gatgctgcca
    22681 tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag cggcctctga
    22741 taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat ctgccagccc
    22801 cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga gatcgcatcg
    22861 ccggtgccgc cttcggcctt cactgcgttg atcatgtaat agctgaaacc aagataatcg
    22921 caggtgcctt cacgcagtac atccagatcg ccatcttcca ttttgatgtt aaatccgcga
    22981 cgctcccact cgttcaacac ataggacggg taatagccgc gtagctgcac atcggtaaag
    23041 acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt acaggagtaa
    23101 ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt aatgcgacgc
    23161 gcggctttca ctgctagggc gctggcgaca aactggtgat gcagcacctg gtacatcgtc
    23221 tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc aaacagcggc
    23281 gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt gactttatgc
    23341 ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgac tttacggttg
    23401 gtccagctac cgtattgctg caccagatgc agcggtattt caaagtggga gagggttatc
    23461 accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa cttcagccct
    23521 tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc gatggaagtc
    23581 cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta gtgaccataa
    23641 aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt ggtgatttcg
    23701 cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggtcc tttgccgcct
    23761 ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa gaaatctttc
    23821 ggtaaggtga gttttttcac tatcataggc tccagatgag tcaattctta caggaaatag
    23881 taccatgcag ccaaacgaca tcactttttc tcaacgtttc caggatgaca ttctggctgg
    23941 gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac ttcaaaacgg gtgatgtgct
    24001 tcgtgtcgga cgttttgaag atgacggtta tttttgcacg attgaagtca ccgcaacctc
    24061 aaccgtaacg ctggatacgc tgacggaaaa acatgcagag caggaaaata tgaccctgac
    24121 tgaactgata aaggtcattg ccgacatcta tcccggtcag acacagtttt atgtgattga
    24181 atttaaatgt ctttaaattg aactcacgcc tgttagttga aatttctcat ttaatttcat
    24241 gatttttagg agattagtct caaatcagaa tttattttag ctaacaggtg ttcactggaa
    24301 ctattctcag ttacgctgga gaggtataca tgtgcgtgta cgtttctccg gagtaagtta
    24361 tggttcagaa gcccctcatt aagcagggat attcgctggc agaggaaatt gccaacagcg
    24421 tcagtcacgg cattgggttg gtgtttggta tcgttgggct ggtgttgcta ctggttcagg
    24481 cggtggatct caatgccagc gccacagcga taaccagcta cagcctctat ggcggcagta
    24541 tgatcctgct gttcctcgct tcgacgctct atcacgccat tcctcatcaa cgggcaaaaa
    24601 tgtggctgaa gaaatttgac cattgcgcta tttacctgtt gattgccgga acctacacgc
    24661 cgtttttgct ggtggggctg gattctccgt tagcgcgcgg gttgatgatt gttatctgga
    24721 gcctggcatt gctggggatt ctgtttaaac tgaccatcgc gcaccgattc aaaattttat
    24781 ctctggtgac ctatctggcg atgggctggc tgtcgctggt ggtaatttat gaaatggcag
    24841 ttaagctcgc ggcgggcagc gttaccttac tggcggtagg cggcgtggtt tattcgctcg
    24901 gagtgatttt ctatgtctgc aaacgcattc cctacaatca tgccatctgg cacggcttcg
    24961 tgctcggcgg cagtatgtgc cactttctgg cgatctattt gtatattggg caggcgtaat
    25021 gggtatgcct ttgtaggcct gataagacgc gccagcgtcg catcaggcat cggagcgcaa
    25081 ccgccggatg cggcgtgaac gccttatccg atctacgggc ctcgtccctg ttgtgataaa
    25141 ggcatcaaat cccagacatg ataagacgcg tcagcgtcgc atcaggcgat aaatgcctga
    25201 tgcgatacag agatttactc ttcgagtgaa tacggcagcg gctcgatatg cagtgtattc
    25261 gcatcgtcgc gtacgcggaa gatgctgtcc ggttccatat cgttattcat gacgacctgt
    25321 accacgacct gaccatcttc cagttttacc gcagccagca ccgtaccggt acgacgccag
    25381 ttctcgccca ttttcagctc taagtcttca ccagcttccg gcagtcggct ggcgctacct
    25441 gccagcaacc agagcgcacg tttattggca ccacggaatt ttgctcgcgc caccatctct
    25501 tgtccggtat aacagccttt cttaaaactg ataccgccca gcgcctggag attggtcgcc
    25561 tgtgggataa actgcccgct gttcgcggca tcaatcaccg ggaaacccgc ttcaatgttt
    25621 aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt atcggtcagc
    25681 atattggcag tagcttcatc ggttacgatc aggaaacgtt ctgccgggtg ttcaaaccat
    25741 agcaaagtgg tcgcgccttc tttaactacc tgtttttctc tcgaaggtag ttcactaaag
    25801 agattggcga gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac acgctcgtcg
    25861 tctgccgcga tggtcacttt agagaacacc gcatattttt tcagttcagt cagctgcggt
    25921 tcacgcacgc tgcgccgttc aatccacgca aagccatcgc cgtcgcggaa cagacgtaaa
    25981 ttgctccaca ttttaccttt ggcgtcgcaa tgggcggcga gcaggtgctg attttctgtc
    26041 atctggctga catctgctgt cacctgaccc tgcatatatt tttcgctgtc cgcgccagta
    26101 atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg ggcagaagcc
    26161 gtaggctgac ggggaggaaa aggtgtaaaa gccataatag agtcctgagt tgcttaacgg
    26221 cgtattaata actaatggta aaagagctat gaggcaatgc aagtgctttc actacgcgat
    26281 ctgtgccttc tgcgaggcga cttccaaaaa aatgaagctc ttgaattgtt gtgaattaat
    26341 ttcgcgatcg cgatggtgtt tactgatatt cccgtaaaag aagtcgaaaa acacgttaca
    26401 atagccgggt actggatttt cgtgagacag gaagaagatg gacattaaca acaaagcccg
    26461 cattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca tgccgttttt
    26521 cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc gtctgctgga
    26581 atgtgacgat ccggacctgt ttaactggct gatgaatcac ggtaaaccgg ccgatgcaga
    26641 actggaaatg atggtccgac tcatccagac acggaaccgg gaacgtggtc ctgtggcaat
    26701 ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt gctgattcat gggctggttg
    26761 ccgctgttat tttactcatg ccctggccgc tcagttacac cccgttatgg atggtgttac
    26821 tttcgctggt ggtatttgat tgcgttcgca gccagcggcg tattaatgct cgccaggggg
    26881 aaattcgatt gttgatggac gggcgtttgc gttggcaagg gcaggagtgg agcatcgtca
    26941 aagcaccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat ggcggtaaac
    27001 ggcaacattt atggctggca gccgacagca tggacgaagc tgaatggcgg gatttacggc
    27061 ggattttgtt gcaacaagag acgcaaagat aaatatgacg gcaaagcata tgcgctaagc
    27121 cgtcagtgag atcaggcgta atgctctgcc atttcgttga ggatttgctc gcaccagctc
    27181 tgaatacgct cgtcgctaag gtcatactgg ttagtttcat ccagcgccag acccacgaac
    27241 agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc cgttggccag
    27301 tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatacc gagcgcatcg
    27361 aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaac aattttacct
    27421 tcaaggttca ggtcgtcgag ctgatcccag acggcttccc agtcttcctg gatttcgcca
    27481 aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa tttcggggag
    27541 tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg gattttttct
    27601 gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt cataacgtaa
    27661 gcttctcgat tctgctgtgg cttttgcggc tagtgtatca gaatcgcttt agtcacggca
    27721 caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa caggatctgg
    27781 cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct gaaaatacgt
    27841 tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac cgcgggttga
    27901 cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg ctagagggcg
    27961 ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg ttccagtatc
    28021 tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca ccgaaattgc
    28081 cacagcgttt gccaaaagat ttaagcgaag cgcaggtcga acgtttatta caggcaccat
    28141 taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg tatgctaccg
    28201 ggctgcgtgt ctctgaactg gtcgggctga caatgagtga tatcagccta cgtcagggcg
    28261 tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt gccgttaggt gaagaggccg
    28321 tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgttgaat ggtgtgtcaa
    28381 ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc ttctggcacc
    28441 gcattaaaca ttatgccgtg ctggcgggta tcgacagcga aaagctgtca ccgcatgtgt
    28501 tgcgtcacgc ttttgccact catttattaa atcatggtgc ggatttacgc gtggtgcaga
    28561 tgctactggg ccacagcgat ctctccacca cgcaaattta tacgcatgtc gctaccgagc
    28621 gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa agaacgggaa
    28681 gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca ggctttgctc
    28741 aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcattaaa agcagcgata
    28801 ttcagccagc acccgtggct ggcatgaaga ctgtgctgac taacagcggt gtgttgtaca
    28861 tcaccgatga cggtaaacat atcattcagg ggccaatgta tgacgttagt ggcgcggctc
    28921 cggtcaatgt caccaataaa atgctgctaa agcagttgaa tgcgcttgaa aaagagatga
    28981 tcgtttataa agcgccgcag gaaaaacacg tcatcaccgt gtttactgat attacctgtg
    29041 ggtactgcca caaactgcat gagcaaatgg cagactacaa cgcgctgggg atcaccgtgc
    29101 gttatcttgc tttcccgcgc caggggctgg agagccaggc tgagcaacaa atgaaagcca
    29161 tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt gatggctggt aaagctgcca
    29221 ctccggcaag ttgcgatatc gatattgccg atcattacgc acttggcgtc cagcttggcg
    29281 ttagcggcac tccggcaatt gtgctgagca atggcacact tgttccgggt taccagccgc
    29341 cgaaagagat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt aattaattcg
    29401 cgtgaaacaa cagatacaac ttcgtcgccg tgaagtcgat gaaacggcag acttgcccgc
    29461 tgaattgcct cccttgctgc gccgtttata cgccagccgg ggagtacgca gtgcgcaaga
    29521 actggaacgc agtgttaaag gtatgttgcc ctggcagcaa ctaagcggtg tcgaaaaggc
    29581 cgttgagatc ctttacaacg cttttcgcga aggaacgcgg attattgtgg tcggtgattt
    29641 cgacgccgac ggcgcgacca gcacggctct aagcgtgctg gcgatgcgct cgcttggttg
    29701 cagcaatatc gactacctgg taccaaaccg tttcgaagac ggttacggct taagcccgga
    29761 agtggtcgat caggcccatg cccgtggcgc gcagttaatt gtcacggtgg ataacggtat
    29821 ttcctcccat gcgggggttg aacacgctcg ctcgttgggc attccggtta ttgttaccga
    29881 tcaccatttg ccaggcgaaa cattacccgc ggcggaagcg atcattaacc ctaacttgcg
    29941 cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt atctgatgct
    30001 ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgcggca tcgcaattcc
    30061 taacctggca gaactgctgg atctggtcgc gctggggaca gtggcggacg tcgtgccgct
    30121 ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgtg ccggaaagtg
    30181 ccgtccaggg attaaagcgc tgctggaagt ggcaaaccgt gatgcacaaa aactcgccgc
    30241 cagcgattta ggttttgcgc tggggccacg tctcaatgct gccgggcgac tggacgatat
    30301 gtccgtcggt gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg tgctggcaaa
    30361 tgaactcgat gcgctaaatc agacgcgaaa agagatcgaa caaggaatgc agattgaagc
    30421 cttgaccctg tgcgagaaac tggagcgaag tcgcgacacg ctacccggcg ggctggcaat
    30481 gtatcacccc gaatggcatc agggcgttgt cggtattctg gcttcgcgca tcaaagagcg
    30541 ttttcaccgt ccggttatcg cctttgcgcc agcaggtgat ggtacgctga aaggttccgg
    30601 tcgctccatt caggggctgc atatgcgtga tgcgctggag cgattagaca cactctaccc
    30661 cggtatgatg ctgaagtttg gcggtcatgc gatggcggcg ggtttgtcgc tggaagagga
    30721 taaattcgaa ctctttcaac aacggtttgg cgagctggtt accgagtggc tggacccttc
    30781 gctattgcaa ggcgaagtgg tgtcagacgg cccgttaagc ccggccgaaa tgaccatgga
    30841 agtggcgcag ctgctgcgcg atgctggccc gtgggggcag atgttcccgg agccgctgtt
    30901 tgatggtcat ttccgtctgc tgcaacagcg gctggtgggc gaacgtcatt tgaaagtcat
    30961 ggtcgaaccg gtcggcggcg gtccgctgct ggatggtatt gcttttaatg tcgataccgc
    31021 cctctggccg gataacggcg tgcgcgaagt gcaactggct tacaagctcg atatcaacga
    31081 gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa tttagcgtca
    31141 tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac cgcattttga
    31201 ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata atcgcattca
    31261 ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac gccaagaaag
    31321 agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaac gaacccgaac
    31381 gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac accctcgacc
    31441 aaatgaaaca ggggctggaa gatgtttctg gtctgctgga actggctgta gaagctgacg
    31501 acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa aaactggcgc
    31561 agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgc tacctcgata
    31621 ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt gagcgtatgt
    31681 atttgcgctg ggcagaatcg cgtggtttca aaactgaaat catcgaagag tcggaaggtg
    31741 aagtggcggg tattaaatcc gtgacgatca aaatctccgg cgattacgct tacggctggc
    31801 tgcgtacaga aactggcgtt caccgcctgg tgcgtaagag cccgtttgac tccggcggtc
    31861 gtcgccacac gtcgttcagc tccgcgtttg tttatccgga agttgatgat gatattgata
    31921 tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc gcgggcggtc
    31981 agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca catcccgacc gggatcgtga
    32041 cccagtgcca gaacgaccgt tcccagcaca agaataaaga ccaggccatg aagcagatga
    32101 aagcgaagct ttatgaactg gagatgcaga agaaaaatgc tgagaaacag gcgatggaag
    32161 ataacaaatc cgacatcggc tggggcagcc agattcgttc ttatgtcctt gatgactccc
    32221 gcattaaaga tctgcgcacc ggggttgaaa cccgcaatac gcaggccgtg ctggacggca
    32281 gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc aacatgtctg
    32341 aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg aaaacgcgtc
    32401 gtgagaagct ggcgaacctg cgtgagcagg gaattgcctt cccgaacgat ttccgtcgcg
    32461 atcatacctc tgaccaattg cacgcagaat tcgacggtaa agagaacgaa gaactggaag
    32521 cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt atgggtaaag
    32581 cctcttttgt taccctgcag gacgttggcg gtcgtattca gctgtacgtt gcccgtgacg
    32641 atcttccgga aggcgtttac aacgagcagt tcaaaaaatg ggatctcggc gacatcctcg
    32701 gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac tgcaccgagc
    32761 tgcgtctgct gaccaaagca ctgcgtccgc tgccggacaa attccacggc ttgcaggatc
    32821 aggaagcgcg ctatcgtcag cgttatctgg atctcatctc taacgatgaa tcccgcaaca
    32881 cctttaaagt gcgttcgcag atcctctctg gcattcgcca gttcatggta aatcgcggct
    32941 ttatggaagt tgaaacgccg atgatgcagg tgatcccagg cggtgccgct gcgcgtccgt
    33001 ttatcaccca tcataacgcg ctggatcttg acatgtacct gcgtatcgcg ccggaactgt
    33061 acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac cgtaacttcc
    33121 gtaacgaagg tatttccgta cgtcataacc cagagttcac catgatggaa ctctatatgg
    33181 cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt actctggcac
    33241 aggatattct cggtaagacg gaagtcacct acggcgacgt gacgctggac ttcggtaaac
    33301 cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa accgacatgg
    33361 cggatctgga caacttcgac tctgcaaaag cgattgctga atctatcggc atccacgttg
    33421 agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg gcagaagcac
    33481 atctgattca gccgaccttc attactgaat atccggcaga agtttctccg ctggcgcgtc
    33541 gtaacgacat taacccggaa atcacagacc gctttgagtt cttcatcggt ggtcgtgaaa
    33601 tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcagcgc ttcctggatc
    33661 aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgacgaa gattacgtca
    33721 ccgcactgga acacggctta ccgccgacag caggtctggg aattggtatc gaccgtatgg
    33781 taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg gcgatgcgtc
    33841 cagtaaaata agcattacgt tatgctaata accccggcaa atgtcggggt tttttaattg
    33901 tgctgcgcga aagcagacaa caattttctt gcttcactat tggctgcctg cattaccatc
    33961 cacggactga acgcccacgg cgtggcatca ataccgtgta atacatctgc taaatcacac
    34021 cattgataat ccatcacttc atcatcgttg atctgtaacg cactgttggt gcgtgcggca
    34081 aataccggac acacttcatt ttccacaatg ccattcggat cggtggcgcg atagcgaaag
    34141 tcaggataga tagattcagg aggcgtaatt tccacgccaa gctcataacg gcaacggcgg
    34201 atcactgcgt cttcgttgct ttctcccagt tgtgggtgcc cacaaaccga gttagtccac
    34261 acgccaggcc atgctttttt gctcagtgcg cggcgggtaa ctaataattg ccccttggca
    34321 ttaaacagcc aactggagaa cgcgagatgt aagagggtgt ctgccgtgtg tgcggcatac
    34381 ttttccagcg tacccgtggg aactccctgt gcattcaata aaatgacgtg ttccgtttgc
    34441 ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag agtgaaatga
    34501 ttaaccaagt gagcgtttat cgacaaccgc ccgttttgag cggatgccga caggtaaaaa
    34561 ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt gcaacacagt
    34621 cagaaatgac aggaatagag agagtaaaaa gcaacagcag actgaatata gcgcgcatta
    34681 aaagatttat attattctcc atgctcattt ttcttcagct tgcgcatcag aagccacata
    34741 cgtactgtcc tgacttttaa atctttatca gagacggatt ccttcaccag gtcagcatga
    34801 tgctgatagc cattaaagaa gacgttcagc acaacggcgc taagggttgc gagcataatg
    34861 ccgctatgta gcagcggttg cagtacggcc ggtaacttag aaaagaaatc gtgagagagc
    34921 gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta acggttggtg
    34981 gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc gaacatcact
    35041 agaccagcgc cgcccagcac aaattgcgga atggaggcta ccagcaccgc catttttggc
    35101 accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg gctatgaacg
    35161 cgcgtcacgc taaccaggcc aacgttttga gaaaatgacg tgtgggggaa gctgttaaac
    35221 gtgccgccta tcattgtccc tacgccatcg acacgcagcc cgcgaataat atcgtgtgaa
    35281 gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga ctcgataaac
    35341 acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat cggcatacca
    35401 aacgacatcg gggtgacaat cgcaaaccac aaggcatcat gtagcccgga taaattgact
    35461 tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac ggcgacgttg
    35521 gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac ggcaaaggag
    35581 atacctgaat aaacgggatt accatattgc ggattccctt tacctccggc ggcccagtca
    35641 atacccacct gaatgatgct aagtccgaca gaagtaataa ccacaccggt aaccagtggc
    35701 gggaataaag gcatcaagcg accgataagt ggcgctaata atgtggtgat aaaacccgcg
    35761 gcgatagtgg caccaaatat ccccagcagg ccaatatccg ggttcatccc aatggctatc
    35821 attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag
    35881 cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta
    35941 atgagcatcg caatagcttc ttttgagagg cccagtcggt caccaatcat aagaggaaca
    36001 gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct
    36061 ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagagaagt
    36121 tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa aaagcaaccg
    36181 tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt gcgaggaagg
    36241 ggagttagag acaggaattg cggaggcaat cacataatcg tggttaaacc acgaaaatga
    36301 gaagagaacc tgacaatgga attatctgta attttctcat attgatattt cattaatcat
    36361 tctggcatgt cgtactctcg catttaatcg tttttatctg gatagcactc ttttgatcgg
    36421 caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac tcagtcgcaa
    36481 aattataatt aaaacggtca tgctacctaa ta
//
LOCUS       DAJVOY010000018        36243 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVOY010000018 DAJVOY010000000
VERSION     DAJVOY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.018"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            584..670
                     /gene="leuL"
                     /locus_tag="OQL90_000821"
     CDS             584..670
                     /gene="leuL"
                     /locus_tag="OQL90_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1367689.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            763..2334
                     /gene="leuA"
                     /locus_tag="OQL90_000822"
     CDS             763..2334
                     /gene="leuA"
                     /locus_tag="OQL90_000822"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1367690.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2334..3425
                     /gene="leuB"
                     /locus_tag="OQL90_000823"
     CDS             2334..3425
                     /gene="leuB"
                     /locus_tag="OQL90_000823"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1367691.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3428..4828
                     /gene="leuC"
                     /locus_tag="OQL90_000824"
     CDS             3428..4828
                     /gene="leuC"
                     /locus_tag="OQL90_000824"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1367692.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4839..5444
                     /gene="leuD"
                     /locus_tag="OQL90_000825"
     CDS             4839..5444
                     /gene="leuD"
                     /locus_tag="OQL90_000825"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1367693.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPVIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5493..6575)
                     /locus_tag="OQL90_000826"
     CDS             complement(5493..6575)
                     /locus_tag="OQL90_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1367694.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYEDNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6676..6807)
                     /gene="sgrT"
                     /locus_tag="OQL90_000827"
     CDS             complement(6676..6807)
                     /gene="sgrT"
                     /locus_tag="OQL90_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1367695.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6896..8551
                     /gene="sgrR"
                     /locus_tag="OQL90_000828"
     CDS             6896..8551
                     /gene="sgrR"
                     /locus_tag="OQL90_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1367696.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSTLRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8585..8684
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8715..9698
                     /gene="thiB"
                     /locus_tag="OQL90_000829"
     CDS             8715..9698
                     /gene="thiB"
                     /locus_tag="OQL90_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1367697.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9674..11284
                     /gene="thiP"
                     /locus_tag="OQL90_000830"
     CDS             9674..11284
                     /gene="thiP"
                     /locus_tag="OQL90_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1367698.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11268..11966
                     /gene="thiQ"
                     /locus_tag="OQL90_000831"
     CDS             11268..11966
                     /gene="thiQ"
                     /locus_tag="OQL90_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1367699.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(12079..12843)
                     /locus_tag="OQL90_000832"
     CDS             complement(12079..12843)
                     /locus_tag="OQL90_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1367700.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12929..13807)
                     /gene="araC"
                     /locus_tag="OQL90_000833"
     CDS             complement(12929..13807)
                     /gene="araC"
                     /locus_tag="OQL90_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1367701.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14146..15846
                     /gene="araB"
                     /locus_tag="OQL90_000834"
     CDS             14146..15846
                     /gene="araB"
                     /locus_tag="OQL90_000834"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1367702.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15857..17359
                     /gene="araA"
                     /locus_tag="OQL90_000835"
     CDS             15857..17359
                     /gene="araA"
                     /locus_tag="OQL90_000835"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1367703.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQHLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17559..18254
                     /gene="araD"
                     /locus_tag="OQL90_000836"
     CDS             17559..18254
                     /gene="araD"
                     /locus_tag="OQL90_000836"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1367704.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18329..20680
                     /gene="polB"
                     /locus_tag="OQL90_000837"
     CDS             18329..20680
                     /gene="polB"
                     /locus_tag="OQL90_000837"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1367705.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20844..23750
                     /gene="rapA"
                     /locus_tag="OQL90_000838"
     CDS             20844..23750
                     /gene="rapA"
                     /locus_tag="OQL90_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1367706.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23762..24421
                     /gene="rluA"
                     /locus_tag="OQL90_000839"
     CDS             23762..24421
                     /gene="rluA"
                     /locus_tag="OQL90_000839"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1367707.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24538..25353)
                     /gene="djlA"
                     /locus_tag="OQL90_000840"
     CDS             complement(24538..25353)
                     /gene="djlA"
                     /locus_tag="OQL90_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1367708.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25608..27962
                     /gene="lptD"
                     /locus_tag="OQL90_000841"
     CDS             25608..27962
                     /gene="lptD"
                     /locus_tag="OQL90_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1367709.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28015..29301
                     /gene="surA"
                     /locus_tag="OQL90_000842"
     CDS             28015..29301
                     /gene="surA"
                     /locus_tag="OQL90_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1367710.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29301..30290
                     /gene="pdxA"
                     /locus_tag="OQL90_000843"
     CDS             29301..30290
                     /gene="pdxA"
                     /locus_tag="OQL90_000843"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1367711.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30287..31108
                     /gene="rsmA"
                     /locus_tag="OQL90_000844"
     CDS             30287..31108
                     /gene="rsmA"
                     /locus_tag="OQL90_000844"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1367712.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            31111..31488
                     /gene="apaG"
                     /locus_tag="OQL90_000845"
     CDS             31111..31488
                     /gene="apaG"
                     /locus_tag="OQL90_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1367713.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31495..32337
                     /gene="apaH"
                     /locus_tag="OQL90_000846"
     CDS             31495..32337
                     /gene="apaH"
                     /locus_tag="OQL90_000846"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1367714.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32415..32894)
                     /gene="folA"
                     /locus_tag="OQL90_000847"
     CDS             complement(32415..32894)
                     /gene="folA"
                     /locus_tag="OQL90_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1367715.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33086..34948)
                     /gene="kefC"
                     /locus_tag="OQL90_000848"
     CDS             complement(33086..34948)
                     /gene="kefC"
                     /locus_tag="OQL90_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1367716.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34941..35471)
                     /gene="kefF"
                     /locus_tag="OQL90_000849"
     CDS             complement(34941..35471)
                     /gene="kefF"
                     /locus_tag="OQL90_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1367717.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35579..36243)
                     /locus_tag="OQL90_000850"
                     /pseudo
     CDS             complement(35579..36243)
                     /locus_tag="OQL90_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8799 a         9908 c         9546 g         7990 t
ORIGIN      
        1 catgcatata aaataagaaa aagcaaaatg agtaaaattc ggaaaacata aagatacggg
       61 ttctgataaa aacagaatcc attgccgtgg aatgagtcat ttaccacagc ataataatcc
      121 ataatgtttt tgttgcgaaa acaatctaat cataactacc gcgaatactc aatcatctac
      181 aaaatggatt aaatgtgctt atttaataat taatttatat atttaatgca ttaattatta
      241 acattaatgg atcaataata ttcaccatat caatatcaaa aaaaattcgc aaaacatata
      301 attcaataca aatcatcagg ataggttttg caacgcgtgc attttgtccc ctttttcctc
      361 gttgattaga tgcaaaaatt tattctgaaa tatgtcaacc tatgaaaagt gtcggtcgtt
      421 aaacagaaat taatatcgct tgctttaacc accgcagcac aattagctaa ttttacggat
      481 gcagaactcg cgctggcggg acgtttttat tgcgtcaggg ttgacatccg tttttgtatc
      541 cagtaactct aaaagcatat cgcattcatc tggagctgat ttaatgactc acatcgttcg
      601 ctttatcggt ctactactac taaacgcatc ttctttgcgc ggtagacgag tgagcggcat
      661 ccagcattaa gccagcacgc agtcaaacaa aaaacccgcg ccattgcgcg ggttttttta
      721 tgcccgaagc gaggcgctct aaaagagaca aggacccaaa ccatgagcca gcaagtcatt
      781 attttcgata ccacattgcg cgacggtgaa caggcgttac aggcaagctt gagtgtgaaa
      841 gaaaaactgc aaattgcgct ggcccttgag cgtatgggtg ttgacgtgat ggaagtcggt
      901 ttccccgtct cttcgccggg cgattttgaa tcagtgcaaa ccatcgcccg ccaggttaaa
      961 aacagccgcg tatgcgcgtt agctcgctgc gtggagaaag atatcgacgt ggcggccgaa
     1021 tccctgaaag tcgccgaagc cttccgtatt cataccttta ttgccacttc gccaatgcac
     1081 atcgccacca agctgcgcag cacgctggat gaagtgatcg aacgcgctat ctatatggtg
     1141 aaacgcgccc gtaattacac cgatgatgtt gaattttctt gcgaagatgc cgggcgtacc
     1201 cccattgccg atctggcgcg agtggtcgaa gcggcgatta atgccggtgc caccaccatc
     1261 aacattccgg acaccgtggg ctacaccatg ccgtttgagt tcgccggaat catcagcggt
     1321 ctgtatgaac gcgtacctaa catcgacaaa gccattattt ccgtacatac ccacgacgat
     1381 ttaggcctgg cggtcggcaa ctcactggcg gcggtacatg ccggtgcacg ccaggtggaa
     1441 ggtgcaatga acgggatcgg cgagcgtgcc ggtaactgtt cgctggaaga agtcatcatg
     1501 gcgattaaag ttcgtaagga tattctcaac gtccacaccg ccattaatca ccaggagata
     1561 tggcgcacca gccagttagt tagccagatt tgtaatatgc cgatcccggc aaacaaagcc
     1621 attgttggca gcggcgcatt cgcccactcc tccggtatcc accaggatgg cgtgctgaaa
     1681 aaccgcgaaa actacgaaat catgacacca gaatctattg gtctgaacca aatccagctg
     1741 aatctgacct ctcgttcggg gcgtgcggcg gtgaaacatc gcatggatga gatggggtat
     1801 aaagaaagtg aatataattt agacaatttg tacgatgctt tcctgaagct ggcggacaaa
     1861 aaaggtcagg tgtttgatta cgatctggag gcgctggcct tcatcggtaa gcagcaagaa
     1921 gagccggagc atttccgtct ggattacttc agcgtgcagt ctggctctaa cgatatcgcc
     1981 accgccgccg tcaaactggc ctgtggcgaa gaagtcaaag cagaagccgc caacggtaac
     2041 ggtccggtcg atgccgtcta tcaggcaatt aaccgcatca ctgactataa cgtcgaactg
     2101 gtgaaataca gcctgaccgc caaaggtcac ggtaaagatg cgctgggtca ggtggatatt
     2161 gtcgccaact acaacggtcg ccgcttccac ggcgtcggcc tggccaccga tattgtcgag
     2221 tcctccgcca aagccatggt gcacgtactg aacaatatct ggcgtgccgc agaagtcgaa
     2281 aaagagttgc aacgcaaagc tcaacacaac gaaaacaaca aggaaaccgt gtgatgtcga
     2341 agaattacca tattgccgta ttgccggggg acggtattgg tccggaagtg atgacccagg
     2401 cgctgaaagt gctggatgcc gtgcgcaacc gctttgcgat gcgcatcacc accagccatt
     2461 acgatgtagg cggcgcagcc attgataacc acgggcaacc actgccgcct gcgacggttg
     2521 aaggttgtga gcaagccgat gccgtgctgt ttggctcggt aggcggtccg aaatgggaac
     2581 atttaccacc agaccagcaa ccagaacgcg gcgcgctgtt gcctttgcgt aagcacttca
     2641 aattattcag caacctgcgt ccggcaaaac tgtatcaggg gctggaagca ttctgtccgc
     2701 tgcgtgctga cattgccgct aacggcttcg acatcctgtg cgtgcgcgaa ctgaccggcg
     2761 gcatctattt cggtcagcca aaaggccgcg aaggtagcgg acagtatgaa aaagcgtttg
     2821 ataccgaggt gtatcaccgt tttgagatcg aacgtatcgc ccgcatcgcg tttgaatctg
     2881 cccgcaagcg tcgccacaaa gtcacctcaa tcgacaaagc caacgtgctg caatcctcta
     2941 ttttatggcg ggagatcgtt aacgagatcg ccacggaata cccggatgtc gaactggcgc
     3001 atatgtacat cgacaacgcc accatgcagc tgattaaaga tccatcacag tttgacgtcc
     3061 tgctgtgctc caacctgttt ggcgacattc tgtctgacga gtgcgcaatg atcactggct
     3121 cgatggggat gttgccttcc gccagcctga acgagcaagg ttttggtctg tatgaaccgg
     3181 caggcggctc agcaccagat atcacaggca aaaacatcgc caacccgatt gcgcaaattc
     3241 tgtcgctggc actgctgctg cgctacagcc tggatgccga tgatgcggct tgcgccattg
     3301 aacgcgccat taaccgcgca ttagaagaag gcattcgcac cggggattta gcccgtggcg
     3361 ctgccgccgt tagtaccgat gaaatgggcg atatcattgc ccgctatgtg gcagaagggg
     3421 tgtaatcatg gctaaaacgt tatacgaaaa attattcgac gctcacgtag tgtacgaagc
     3481 cgaaaacgaa accccgctgt tatatatcga ccgccacctg gtgcatgaag tgacctcacc
     3541 gcaggcgttc gatggtctgc gcgcccacgg tcgcccggta cgtcagccgg gcaaaacctt
     3601 cgccactatg gatcacaacg tctctaccca gacgaaagac attaatgcct gtggtgaaat
     3661 ggcgcgcatc cagatgcagg agctgattaa aaactgcaaa gaatttggcg tcgaactgta
     3721 tgacctgaat cacccgtatc aggggatcgt ccacgtaatg gggccggaac agggcgtcac
     3781 cttgccgggg atgaccattg tctgcggcga ctcgcatacc gccacccacg gcgcgtttgg
     3841 cgcactggcc tttggtatcg gcacttccga agttgaacac gtactggcaa cgcaaaccct
     3901 gaaacagggc cgcgcaaaaa ccatgaaaat tgaagtccag ggcaaagccg cgccgggcat
     3961 taccgcaaaa gatatcgtgc tggcaattat cggtaaaacc ggtagcgcag gcggcaccgg
     4021 gcatgtggtg gagttttgcg gcgaagcaat ccgtgattta agcatggaag gtcgtatgac
     4081 cctgtgcaat atggcaatcg aaatgggcgc aaaagccggt ctggttgcac cggacgaaac
     4141 cacctttaac tatgtcaaag gccgtttgca tgcgccgaaa ggcaaagatt tcgacgacgc
     4201 cgttgcctac tggaaaaccc tgcaaaccga cgaaggcgca actttcgata ccgttgtcac
     4261 tctgcaagca gaagaaattt caccgcaggt cacctggggc accaatccag gccaggtgat
     4321 ttccgtgaac gacaatattc ccgatccggc ttcgtttgcc gatccggttg aacgcgcgtc
     4381 ggcagaaaaa gcgctggcct atatggggct gaaaccgggc attccgctga ccgaagtggc
     4441 tatcgacaaa gtgtttatcg gttcctgtac caactcgcgc attgaagatt tacgcgcggc
     4501 agcggagatc gccaaagggc gaaaagtcgc gccaggcgtg caggcactgg tggttcccgg
     4561 ctctggccag gtaaaagccc aggcggaagc ggaaggtctg gataaaatct ttattgaagc
     4621 cggttttgaa tggcgcttgc ctggctgctc aatgtgtctg gcgatgaaca acgaccgtct
     4681 gaatccgggc gaacgttgtg catccaccag caaccgtaac tttgaaggcc gccaggggcg
     4741 cggcgggcgc acgcatctgg tcagcccggc aatggctgcc gctgctgctg tgaccggaca
     4801 tttcgccgac attcgcaaca ttaaataagg agcagaccat ggcagagaaa tttatcaaac
     4861 acacaggcct ggtggttccg ctggatgccg ccaatgtcga taccgatgca atcatcccga
     4921 aacagttttt gcagaaagtg acccgtacgg gttttggcgc gcatctgttt aacgactggc
     4981 gttttctcga tgaaaaaggc caacagccaa acccggactt cgtgctgaac ttcccgcagt
     5041 atcagggcgc ttccattttg ctggcacgag aaaacttcgg ctgtggctcc tcgcgtgagc
     5101 acgcgccctg ggcattgacc gactacggtt ttaaagtggt gattgcgccg agttttgctg
     5161 acatcttcta cggcaatagc tttaacaacc agctgctgcc ggtgaaatta agcgatgcag
     5221 aagtggacga actgtttgcg ctggtgaaag ccaatccggt gatccatttc gacgtggatc
     5281 tggaagcgca agaggtgaaa gcgggagaga aaacctatcg ctttaccatc gatgccttcc
     5341 gccgccactg catgatgaac ggtctggaca gtattgggct taccttgcag cacgacgacg
     5401 ccattgccgc ttatgaagca aaacaacctg cgtttatgaa ttaatcccct tgcccggtca
     5461 aatgaccggg ctttccgcta tcgtccacgt catcaaacgt ctttaacctt tgcggttaaa
     5521 aataatgcga caacagaaat aaccgcaatt acccagtaga cagcaaagtg cccccaactt
     5581 tgtgcaattg ctccctgaat aacgccagcc agaattaccc cggtagaaat actgttagta
     5641 aataaggtgg tagctgcccc cgctcttcca ggcattaaat cctgaaacca tagcatccca
     5701 atacccgcaa caatgccgat aaatacagcg ttaaaaagtt gcagcgtcat caacgccata
     5761 cggctatgaa agaaaatcaa tccggtgtaa aacagtactc ctgccgccac tgctatgacc
     5821 atcattcgcc gcttaccata acgtttgaca tagtagccag ccagaatcat tgctggtatt
     5881 tccagtccag ctgccgtccc catcaggaaa cccgccagtt tgtctggcaa tcctaactcg
     5941 ctactgatcc acaacggcat atcaataatg tacatggtgt tgcaggtcca cattaacgtc
     6001 gaggcgacaa ataacatccg tacgttacta tcctgccagc caccttgcat tgataaagca
     6061 ttttccgacg gcagttctac ccgcgccaca gacggaagca taaatgcaat caataccaga
     6121 ctgagtgtga atatcccggc ggcaatcgaa aacatcacca cttctcgcgc cgagttatcc
     6181 tcatattccc gcgccagagc aaataactgt ggcattgccg tattggccag agatgccaga
     6241 agcacaccac aggtgataag cgtcagataa tgacgattaa atgcaaacaa tggcgcattg
     6301 ccgatagcca tcaaacagca aaatataatc agttttcgcc gatcgccctg actgtcagaa
     6361 cgttttgcca accagaggct tacgccgatc ccagcaatag tattcaccgt ataaaagagt
     6421 ccgatccaga aaggttgcgc gccaacctca cgactcagaa ataagctcaa tgtaggagcc
     6481 tgtagcgccc cggccacccc catcataaaa gcgaccagca taaatgccgc gtaaacaccg
     6541 ttcatacggc gagccatcgt cattatccag atcatacgtt ccctttttag cgcggcgaga
     6601 ataaaaaaac cagcaggtat aatctgctgg cgggtgattt tacaccaata ctcagtcaca
     6661 catgatgcag gcaagtcaac tttcagaatt gcggtcatcc cactgcatca gtccttcaag
     6721 catggttaat cgttgtggga cgcttaacca acgcaaccag cacaacttcg ctgtcgcggt
     6781 aaaatagtgc tgataaaact gacgcatggg gcacctcctt gcttcatcgt taggaattat
     6841 tgacttaata tagggaaaat aaaattgctg tcttttgcgc aggagttcct cttttatgcc
     6901 atctgctcgt ctgcaacaac agttcatccg cctgtggcaa tgctgcgagg gtaaatcgca
     6961 ggacacaacg ctcaacgaac tggcagcgtt attgagctgc tcgcgtcgtc atatgcgtac
     7021 cctgctcaac accatgcagg atcgcggctg gctgacgtgg gaagcggaag tcgggcgcgg
     7081 taaacgctcg cgtctgacat tcctctatac cgggctggcg cttcagcaac agcgggcgga
     7141 agacctgctg gagcaggatc gtatcgacca actagtgcag ttggttggcg acaaagcgac
     7201 tgtgcggcaa atgctggttt ctcatctggg ccgcagcttc cgccaggggc ggcacatcct
     7261 gcgcgtgctc tactatcgtc cgttgcgtaa tctgctacct ggcagcacat tgcgccgttc
     7321 cgaaacccat attgcccggc aaatcttcag ttcgctaacg cgcataaatg aggaaaatgg
     7381 ggaactggaa gcagacatcg cccaccactg gcagcaaata tcaccgcttc actggcgttt
     7441 ctttttgcgt ccaggagtcc attttcatca tggtcgtgaa ctggaaatgg acgacgtgat
     7501 cgcctcttta aaacgaatca atacgctgcc gctctattcg catattgcgg acattgtgtc
     7561 gccgacgccc tggacgctgg atatccatct cacgcaaccg gaccgctggt taccgttact
     7621 gctggggcaa gttccggcga tgatcctgcc gcgcgaatgg gaaaccctca gtaactttgc
     7681 cagccatccc atcggcaccg gtccgtatgc ggtgatccgc aacaccacca atcaactgaa
     7741 aattcaggca ttcgatgact tcttcggtta ccgggcatta atcgatgaag ttaacgtctg
     7801 ggttctgccg gaaattgccg acgagccagc tggaggtctg atgctaaaag gtccacaggg
     7861 tgaggaaaaa gagattgaaa gccgcctgga ggaaggttgc tactatttac tattcgacag
     7921 ccgcacccat cgcggagcga atcagcaagt cagggactgg gtaaactatg tgctttctcc
     7981 aactaatctg gtctatttcg ctgaggaaca gtaccagcaa ctgtggttcc cggcttatgg
     8041 actgctcccc cgttggcacc atgcccgcac cataaagagc gaaaaaccgg ctggcctgga
     8101 aagcctcacc ctgacctttt atcaggatca cagtgagcat cgggtgattg ccgggatcat
     8161 gcagcagatt ctggcaagtc accaggtcac gctggaaatc aaagagatca gctacgatca
     8221 gtggcatgaa ggagagatcg agagcgatat ctggcttaat agcgccaact ttacgctgcc
     8281 gctggatttt tcgctgttcg cgcacttgtg cgaggtgccg ctgctccaac actgtattcc
     8341 gatcgactgg caagccgacg ccgcccgctg gcgcaatggc gaaatgaacc tggcgaactg
     8401 gtgccagcaa ctggtcgcca gtaaagcaat ggtgccgctt atccaccact ggctgatcat
     8461 tcagggacaa cgcagtatgc gcggcctgcg tatgaatacc ctcggctggt tcgattttaa
     8521 atcagcgtgg tttgcgccgc cggatccatg attgctggta gataacaaaa tcactacact
     8581 aacgccgttc tcaacggggt gccacgcaaa cgcgtgcgct gagaaaatac ccgtcgaacc
     8641 tgatccggat aacgccggcg aagggatttg aggctccttc tcaagtcctt tgccactctt
     8701 ttttgaggtg caaagtgtta aaaaaatgtc ttcccctgct gttgctgtgc acagcgcccg
     8761 ttttcgctaa acccgttctg actgtttata cctacgattc cttcgccgcc gactgggggc
     8821 ctggtccaaa gatcaaaaaa gcttttgaag ccgactgtaa ctgcgaactg aaactggtgg
     8881 cgctggaaga tggcgtttcg cttctcaacc gtctacggat ggaaggcaaa aatagtaaag
     8941 ccgatgtggt gctggggctg gataacaatc tgttagacgc cgccagtaaa accggactat
     9001 ttgccaaaag cggtgtggca gcggatgccg ttaacgttcc cggcggctgg aataatgaca
     9061 ctttcgtccc gtttgattac ggctatttcg ccttcgtcta tgacaagaac aaactgaaaa
     9121 acccgccgca aagcctgaaa gaattggttg agagcgatca aaactggcgg gtgatttatc
     9181 aggatccgcg caccagtacg ccggggctgg gcctgctgtt gtggatgcaa aaagtctatg
     9241 gcgatgacgc cccacaagcc tggcaaaaac tggcgaagaa aaccgtcacg gtcaccaaag
     9301 gctggagcga agcctacggc ctgtttttaa aaggtgaaag cgatctggta ctgagttaca
     9361 ccacctctcc ggcttatcac attctcgaag agaaaaaaga taactacgcc gccgcgaact
     9421 ttagcgaagg tcactatctg caggtggaag tcgccgcccg caccgctgcc agcaagcagc
     9481 cggagctggc gcaaaaattc ctccagttta tggtttctcc ggcttttcag aatgcgatcc
     9541 caaccggtaa ctggatgtat ccggtggcaa atgtcacgct gcctgccggg tttgaacaat
     9601 tgaccaaacc ggccaccacg ctggagttca caccagctga agtggcggca caacgtcagg
     9661 catggattag cggatggcaa cgcgccgtca gccgttaatt cccggctggt taattccagg
     9721 tgtaagcgcc gccacgctgg tggtagcggt tgcgctcgcg gcgtttctcg ccctgtggtg
     9781 gaacgcgccg cagggtaact gggtggcagt ctggcaggac agctacctgt ggcatgtggt
     9841 gcgcttctcc ttctggcagg cgtttctctc ggcgctactc tctgtcgtac ccgcgatatt
     9901 cctcgcccgc gccctctatc gcaggcgctt tccgggtcgg ctggcgctgt tgcgtctgtg
     9961 cgcaatgacc ttgatcctcc cggtgctggt tgctgttttc ggcattctta gcgtctatgg
    10021 tcgccagggc tggctggcat cgctctgcca atcgctcggt ctggagtgga ccttttcgcc
    10081 ctacggcctg caaggtattt tgctggcgca cgtatttttt aatctgccga tggcgagccg
    10141 cttattactc caggcactgg aaaacattcc cgacgaacaa cgtcagcttg ccgcccagct
    10201 tgggatgcgc ggctggcatt ttttccgctt cgtcgaatgg ccgtggttac ggcgacaaat
    10261 cccgccggtt gctgcactta tctttatgct ctgtttcgcc agcttcgcca ccgtgctatc
    10321 actgggcggc ggtccgcagg cgaccactat cgagctggct atttatcagg cgctgagtta
    10381 cgactacgat cctgcccgcg cggcgatgct ggcgctgatc cagatggtgt gttgccttgg
    10441 gctggtactg ttgagtcagc gattgagtaa ggccattgcg ccaggcacca cgctgctgca
    10501 aggctggcgc gacccggacg atcgtctgca tagccgcatt tgcgacacgg tgttaatagt
    10561 gctggcgttg ctgctgttgt tgccaccgtt gctggcggtg atcgtcgatg gggtaaatcg
    10621 ccagttgccg gaagtgctgg cacaaccggt gctgtggcag gcgctgtgga cctcgttgcg
    10681 tattgcgctg gcggcaggtg tattgtgcgt agtgctgacc atgatgctgc tatggagcag
    10741 tcgcgaactt cgggcacggc agaaaatgct ggcgggtcag gcgctggaga tgagcggcat
    10801 gttgattctc gccatgccgg ggattgtgct ggctaccggc ttctttttac tgctcaacaa
    10861 cactatcggc ctgcaacaat ctgctgacgg cattgtgatt ttcaccaatg cgttaatggc
    10921 gatcccttat gcgctgaaag tactggaaaa cccgatgcgc gatatcaccg cccgctacag
    10981 tatgttgtgt cagtcgctgg gcattgaagg ctggtcgcgc ttaaaagtgg tggagctgcg
    11041 cgccctgaaa cgtccactgg cgcaggcgct ggcctttgcc tgcgtgctgt cgattggtga
    11101 ttttggcgtg gtggcgttgt tcggtaacga tgatttccgt accctgccgt tttatctcta
    11161 ccagcaaatt ggctcctatc gcagccagga cggcgcggtc accgcgttaa ttctgctgct
    11221 actctgtttt ctgctgttta ccgtgattga aaaactaccg gggcgaaatg ttaaaactga
    11281 ctgatatcac ctggctttac caacatttgc cgatgcgttt tagcttaacg gtggaacgcg
    11341 gcgagcaggt ggcgatcctc gggccaagcg gcgcaggtaa aagtaccctg ctgaatttga
    11401 ttgccggttt tctgacgcca gccagcggtt cgctgactat cgatggcgtt gatcacacca
    11461 cgacgccgcc gtcacgccgt ccggtgtcga tgctgtttca ggagaacaac ctgttcagcc
    11521 acctgacggt cgcgcagaac atcgggctgg ggcttaatcc aggattgaag ctgaacgcgg
    11581 cacagcagga gaaaatgcac gctatcgccc gtcagatggg gattgataat ttaatggcgc
    11641 ggttaccggg cgagctttcc ggcggccagc gacagcgcgt ggcgttagcg cgttgtctgg
    11701 tgcgcgaaca gccgatttta ttgctcgatg aaccgttctc tgcgctcgat ccggcgttac
    11761 gtcaggagat gctgacgctg gtgagcacga gctgccagca gcaaaaaatg acgctattga
    11821 tggtgtcgca cagtgtggaa gatgcggcgc ggatcgccac acgctcggtg gtggtcgccg
    11881 acgggcgcat cgcctggcag ggtaagactg aagaattgtt gagcggtaag gcgagtgctt
    11941 cggcaatatt ggggattacg ggttagtatg cactgtcaga tgcggcgtga acaccttatc
    12001 cgacctacgg acagtgcgtt ttgtaggcct gataagaccg gtcagcgtcg catcaggcaa
    12061 cccgcacaag acaatcccct aaaccccaac cactttacgc aaaatatcga tatacaccgg
    12121 catcaacggg tggcgaatta acaccaccag cgccaccacg ccgatggcag aaatcaacgg
    12181 cgtcagccac aacaaacggc cgcgcgacaa ataatgactt aaacgatcgg ttgctttacc
    12241 gctgcgccat aaccgccagc acagccagcc accaacccac aaaaataccg ccgtcgccag
    12301 cagcaaccat ttaaactcgc cgctctgcat tccggctgga atatcgatcg ccgcgcccgc
    12361 cagaatccca ggcaggaagt aaaacggtgg ccacagcagg cagccgataa tattcggcgt
    12421 aatgaatttt gccaccggca gatccagcat tcccgccacc attggcacca gcggacgcgt
    12481 cgggccaaca aagcgaccga ccagaatggt gaacatgctg tgttgatgca acgcatgttc
    12541 agttttatcg agtaatgctt tgtttttctt cagaaatgac cagcgatgca acggcttttt
    12601 aaaacgccag cccagccaga aggagatcca gtcgcccagc agacaaccga caatccctgc
    12661 cagccaggcg tgccagaaac ttaactcgcc gctgccaatc agcgctccca gccccgccat
    12721 cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc gactccagaa aggccaccaa
    12781 caccaccgcc atcaatgaat acacggtaga ttgggtaata aagtgttcca gcaatgcttg
    12841 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
    12901 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
    12961 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
    13021 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
    13081 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
    13141 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
    13201 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
    13261 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
    13321 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
    13381 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
    13441 ccgggcgaaa gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccaat
    13501 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
    13561 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
    13621 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
    13681 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
    13741 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
    13801 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
    13861 gacattgccg tcactgcgtc ttttactggc tcttctcgct aacccaaccg gtaaccccgc
    13921 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
    13981 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
    14041 attccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
    14101 tctctactgt ttctccatac ccgttttttt ggatggagtg aaacgatggc gattgcaatt
    14161 ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg tggactgcgc cagcggtgaa
    14221 gagatcgcca ccagcgtaga gtggtatccc cgttggcaaa aagggcaatt ttgtgatgcc
    14281 ccgaataacc agttccgtca tcatccgcgt gactacattg agtcaatgga agcggcactg
    14341 aaaaccgtgc ttgcagagct tagcgtcgaa cagcgcgcag ctgtggtcgg gattggcgtt
    14401 gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg gtaacgtgct ggcgctgcgc
    14461 ccggagtttg ccgaaaaccc aaacgcgatg ttcgtattgt ggaaagacca cactgcggtt
    14521 gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg gcaacgttga ctactcccgc
    14581 tacattggtg gtatttattc cagcgaatgg ttctgggcaa aaatcctgca tgtgactcgc
    14641 caggacagcg ccgtggcgca atctgccgca tcgtggattg agctgtgcga ctgggtgcca
    14701 gctctgcttt ccggtaccac ccgcccgcag gatattcgtc gcggacgttg cagcgccggg
    14761 cataaatctc tgtggcacga aagctggggc ggcttgccgc cagccagttt ctttgatgag
    14821 ctggacccga tcctcaatcg ccatttgcct tccccgctgt tcactgacac ctggactgcc
    14881 gatattccgg tgggcacctt atgcccggaa tgggcgcagc gtctcggcct gcctgaaagc
    14941 gtggtgattt ccggcggcgc gtttgactgc catatgggcg cagttggcgc aggcgcacag
    15001 cctaacgcac tggtaaaagt tatcggtact tccacctgcg acattctgat tgccgacaaa
    15061 cagagcgttg gcgagcgggc agttaaaggt atttgcggtc aggttgatgg cagcgtggta
    15121 cctggattta tcggtctgga agcaggccaa tcggcgtttg gtgatatcta cgcctggttt
    15181 ggtcgcgtac tcggctggcc gctggaacag cttgccgccc agcatccgga actgaaagcg
    15241 caaatcaacg ccagccagaa acaactgctt ccggcgctga ccgaagcatg ggccaaaaat
    15301 ccgtctctgg atcacctgcc ggtggtgctc gactggttta acggccgccg cacaccgaac
    15361 gctaaccaac gcctgaaagg ggtgattacc gatcttaacc tcgctaccga cgctccgctg
    15421 ctgtttggcg gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat ggagtgcttt
    15481 accgatcagg ggatcgccgt caataacgtg atggcgctgg gcggcatcgc gcggaaaaac
    15541 caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat tgttgcctct
    15601 gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa agtgcacgca
    15661 gacatcccat cagcccagca aaaaatggcc agtgcggtag agaaaaccct gcaaccgcgc
    15721 agcgaacaga cacaacgctt tgaacagctt tatcgccgct atcagcaatg ggcgatgagc
    15781 gccgaacaac actatcttcc aacttccgcc ccggcacagg ctgcccaggc cgttccgact
    15841 ctataaggac acgataatga cgatttttga taattatgaa gtgtggtttg tcattggcag
    15901 ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc caacatgccg agcacgttgt
    15961 taatgcgctg aatacggaag cgaaactgcc ctgcaaactg gtgttgaaac cgctgggcac
    16021 cacgccggat gaaatcaccg ctatttgccg cgacgcgaat tacgacgatc gttgcgctgg
    16081 tctggtggtg tggctgcaca ccttctcccc ggccaaaatg tggatcaacg gcctgaccat
    16141 gctcaacaaa ccgttgctgc aattccacac ccagtttaac gcggcgctgc cgtgggacag
    16201 tatcgatatg gactttatga acctgaacca gactgcacat ggcggtcgcg agttcggctt
    16261 cattggcgcg cgtatgcgtc agcaacatgc cgtggttacc ggtcactggc aggataaaca
    16321 agcccatgag cgtatcggct cctggatgcg tcaggcggtc tctaaacagg atacccgtca
    16381 tctgaaagtc tgccgttttg gcgataacat gcgtgaagta gcggtcaccg atggtgataa
    16441 agttgccgca cagatcaagt tcggtttctc cgtcaatacc tgggcggttg gcgatctggt
    16501 gcaggtggtg aactccatca gcgacggcga tgttaacgcg ctggtcgatg agtacgaaag
    16561 ctgctacacc atgacgcctg ccacacaaat ccacggcgaa aaacgacaga acgtgctgga
    16621 agcggcgcgt attgagctgg ggatgaagcg tttcctggaa caaggtggct tccacgcgtt
    16681 caccaccacc tttgaagatt tgcacggtct gaaacagctt ccaggtctgg ccgtacagca
    16741 tctgatgcag cagggttacg gctttgcggg cgaaggcgac tggaaaaccg ccgccctgct
    16801 tcgcatcatg aaggtgatgt caaccggtct gcagggcggc acctccttta tggaggacta
    16861 cacctatcac ttcgagaaag gtaatgacct ggtgctcggc tcccatatgc tggaagtctg
    16921 cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt cagcatctcg gtattggtgg
    16981 taaggacgat cctgcccgac tgatcttcaa tacccaaacc ggtccagcga ttgtcgccag
    17041 cctgattgat ctcggcgatc gttaccgtct gctggttaac tgtatcgaca cggtgaaaac
    17101 accgcactcc ctgccgaaac tgccggtggc gaatgcgctg tggaaagcgc aaccggatct
    17161 gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg caccataccg tcttcagcca
    17221 tgcgctgaac ctcaacgata tgcgtcagtt cgccgagatg cacgacattg aaatcacggt
    17281 gattgataac gacacccgcc tgccagcgtt taaagacgca ctgcgctgga acgaagtgta
    17341 ttacggattt cgtcgctaag tagccacatc aggtatgtaa cgcctgatgc gacgctgacg
    17401 cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc gcatccggca
    17461 ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct acgcgctgcg attttgtaga
    17521 ccggataagc gaagcgcatc cggcacgaag gagtcaacat gttagaagat ctcaaacgcc
    17581 aggtattaga agccaacctg gcgctgccaa aacacaacct ggtcacgctc acatggggca
    17641 acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt
    17701 acagcgtcat gaccgctgac gatatggtcg tggttagcat cgaaaccggt gaagtggttg
    17761 aaggtacgaa aaagccctcc tccgacacgc caactcaccg gctgctctat caggcattcc
    17821 cctccattgg cggcattgtg catacgcact cgcgccacgc caccatctgg gcgcagtcgg
    17881 gtcagtcgat tccagcaacc ggcaccaccc acgccgacta tttctacggc accattccct
    17941 gcacccgcaa aatgaccgac gcagaaatca acggcgaata tgagtgggaa accggtaacg
    18001 tcatcgtaga aacctttgaa aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg
    18061 tccattccca cggcccgttt gcatggggca aaaatgccga agatgcggtg cataacgcca
    18121 tcgtgctgga agaggtcgct tatatggaga tattctgccg tcagttagcg ccgcagttac
    18181 cggatatgca gcaaacgctg ctggataaac actatctgcg taagcatggc gcgaaggcat
    18241 attacgggca gtaatgactg tataaaacca cagccaatca aacgaaacca ggctatactc
    18301 aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca ggttttatct taacccgaca
    18361 ctggcgggac accccgcaag ggacagaagt ctccttctgg ctggcgacgg acaacgggcc
    18421 gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt attcccgccg atcaggttcc
    18481 ccgcgctcag catattttgc agggtgaaca aggctttcgc ctgacaccgc tggcgttaaa
    18541 ggattttcac cgccagccgg tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa
    18601 ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc
    18661 agaacgctat ctgatggagc ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca
    18721 caatggcact atcgttaatg cccgtctgaa accgcatccc gactatcgtc cgccgctcaa
    18781 gtgggtttct atagatattg aaaccacccg ccacggtgag ctgtactgca tcggcctgga
    18841 aggctgcggg cagcgcatcg tttatatgct ggggtcggag aatggcgacg cctcctcgct
    18901 tgatttcgaa ctggaatacg tcgccagccg cccgcagttg ctggaaaaac tcaacgcctg
    18961 gtttgccaac tacgatcctg atgtgatcat cggttggaac gtggtgcagt tcgatctgcg
    19021 aatgctgcaa aaacatgccg agcgttaccg tcttccgctg cgtcttgggc gcgataatag
    19081 cgagctggag tggcgcgagc acggctttaa aaacggcgtc ttttttgccc aggctaaagg
    19141 tcggctaatt atcgacggta tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt
    19201 ctcgctggaa actgtcgctc aggagctatt aggcgaagga aaatctatcg ataacccgtg
    19261 ggatcgaatg gacgaaattg accgccgttt cgccgaagat aaacctgcgc tggcaactta
    19321 taacctgaaa gattgcgagc tggtgacgca gatcttccac aaaactgaaa tcatgccatt
    19381 tttactcgaa cgggcaacgg tgaacggcct gccggtggac cgacacggcg gttcggtggc
    19441 ggcatttggt catctctatt ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct
    19501 cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg atggattcac ggccagggct
    19561 ttatgattca gtgctggtgc tggactataa aagcctgtac ccgtcgatca tccgcacctt
    19621 tctgattgat cccgtcgggc tggtggaagg catggcgcag cctgatccag agcacagtac
    19681 cgaaggtttt ctcgatgcct ggttctcgcg agaaaaacat tgcctgccgg agattgtgac
    19741 taacatctgg cacgggcgcg atgaagccaa acgccagggt aacaaaccgc tgtcgcaggc
    19801 gctgaaaatc atcatgaatg ccttttatgg cgtgctcggc accaccgcct gccgcttctt
    19861 cgatccgcgg ctggcatcgt cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa
    19921 agcgttgatt gaagcacagg gctacgacgt tatctacggc gataccgact caacgtttgt
    19981 ctggctgaaa ggcgcacatt cggaagaaga agcggcgaaa atcggtcgtg cactggtgca
    20041 gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa cggctgacca gcgcattaga
    20101 actggagtat gaaacccatt tctgccgttt tctgatgcca accattcgcg gagccgatac
    20161 cggcagtaaa aagcgctacg ccgggctgat tcaggagggc gataaacagc ggatggtgtt
    20221 taaagggctg gaaaccgtgc gcaccgactg gacgccgctg gcccagcagt ttcagcagga
    20281 gctatacctg cgcatcttcc gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga
    20341 caaactgatg gcgggtgaac tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc
    20401 gctgagcgag tatcagcgta atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga
    20461 agaaaaccaa aagcgtggtc gccccttgca atatcagaat cgcggcacca ttaagtacgt
    20521 atggaccacc aacggcccgg agccgctgga ctaccaacgt tcaccactgg attacgaaca
    20581 ctatctgacc cgccagctac aacccgtggc ggagggaata ctccctttta ttgaggataa
    20641 ttttgctaca cttatgaccg ggcaacttgg gctattttga gcaaaaaaag agttcgccag
    20701 ataccatttt gatgcgtgac gaatgctttg ccatccagta ccatagcgcc ctttccattc
    20761 ctggacctga ataacaccac tacctcataa gcacggtagc gggtggttat tgcctgcaat
    20821 taaagatata gagccgaaca catatgcctt ttacacttgg tcaacgctgg atcagcgata
    20881 cagaaagcga attgggactt ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac
    20941 ttttcccatc tactggtgaa aaccgtctgt acgcacgcag tgattccccc gtgacccgcg
    21001 tgatgttcaa ccctggtgat accattacca gccatgacgg ctggcagatg caagtcgaag
    21061 aagtaaaaga agaaaatggc ttgctgacct atatcggtac tcgcctggat actgaagagt
    21121 ccggcgtagc cctgcgtgaa gttttccttg atagcaaact ggtgttcagc aaaccgcagg
    21181 accgcctgtt tgccgggcag attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc
    21241 gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca
    21301 gcctgatccc gcaccagctc aacatcgctc atgatgttgg ccgccgccac gcgccgcgcg
    21361 tcctgctggc tgacgaagtg ggtttaggga aaaccattga agccgggatg atcctgcatc
    21421 agcaactgct ctctggcgct gctgaacgtg tgctgattat cgtcccggaa accttacagc
    21481 atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg ctttgctctg tttgatgatg
    21541 agcgttatgc cgaagctcag cacgatgctt acaacccgtt cgacaccgaa cagctggtga
    21601 tttgctcgct ggattttgcc cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg
    21661 aatgggacct gctggtggtc gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa
    21721 gccgtgaata tcaggccatt gaacaactgg cagagcacat gccgggcgtt ctgctgctga
    21781 ccgcgacccc ggaacagctg gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc
    21841 cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca gaaaaattat cgtccggttg
    21901 cggacgccgt tgccatgctg ctggcaggta acaaactgag caatgacgaa ctgaacatgc
    21961 tcggcgagat gatcggcgag caggatatcg agccgctgtt gcaggcagca aacagcgaca
    22021 gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat gctgatggat cgccacggca
    22081 ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa aggattcccg aaacgcgagc
    22141 tgcacaccat taagctgccg ctaccgacgc agtatcagac ggctattaaa gtctccggca
    22201 ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga tatgctctac ccggagcgta
    22261 tttatcagga atttgaaggt gataacgcca cctggtggaa cttcgatccg cgcgttgagt
    22321 ggctgatggg ctacctgacc agccatcgct ctcagaaagt gctggtgatc tgcgccaaag
    22381 ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg
    22441 tgttccacga aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag
    22501 aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc
    22561 agttcgccag ccacatggtg atgtttgacc tgccattcaa cccggatcta ctggagcagc
    22621 gtattggtcg tctggatcgt atcggccagg cgcacgatat tcagatccat gtgccttatc
    22681 tggagaaaac cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg gatgcatttg
    22741 agcacacctg cccgaccgga cgcactattt acgatagcgt atacaacgat ctgattaact
    22801 atctggcttc accggatcaa accgaaggct ttgacgatct gatcaaaaac tgccgcgagc
    22861 aacatgaagc gctgaaagca cagctggaac agggtcgtga ccgcctgctg gaaatccact
    22921 ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat tgaagagcag gatgacgata
    22981 ccaacctgat cgccttcgcc atgaacctgc tcgatattat cggtatcaat caggacgatc
    23041 gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat gctggtgccg gacttccctg
    23101 gcctgtcgga agatggcatc accatcacct ttgatcgtga agtggcgctg gcgcgtgaag
    23161 atgcacagtt tattacctgg gagcatccgc tgatccgcaa cggtctggat ctgatccttt
    23221 ctggcgatac cggtagcagc acgatttcac tgttaaaaaa caaagcgttg ccggtaggta
    23281 cgctgttggt ggaactgatt tatgtggttg aagcccaggc tccgaagcag ttgcagctca
    23341 accgcttcct gccaccgacg ccggtacgta tgctgctgga taaaaacggc aacaacctgg
    23401 cggcgcaggt agagtttgaa acctttaacc gccagcttaa cgcggttaac cgtcacaccg
    23461 gcagcaaact ggttaacgcc gtgcagcagg atgttcacgc tatccttcaa ctgggtgaag
    23521 cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc gcgtaacgaa gccgacgaaa
    23581 aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg aacattcgtg
    23641 acgacgaact gaccgccatt gagagcaacc gtcagcaggt aatggaaagc ctggatcagg
    23701 caggttggcg tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa
    23761 aatggggatg gaaaactaca atccaccgca ggaaccctgg ttggttatcc tgtatcagga
    23821 tgaccacatt atggtggtta acaagccgag cggtttgttg tctgtgccgg ggcgtctgga
    23881 agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt
    23941 gcatcgtctg gatatggcta ccagcggcgt gattgtagtg gcgctgacca aagccgcgga
    24001 gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa aagcagtatg tggcccgcgt
    24061 ctgggggcat ccatcccctg cggaaggtct ggtggatctg ccgctgattt gcgactggcc
    24121 aaaccgcccg aaacagaaag tctgttacga aacgggtaaa cctgcgcaga cggaatatga
    24181 agtggtggag tatgcggcgg ataacacggc aagagtggtg ttaaaaccga ttactgggcg
    24241 ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat ccgattctcg gcgatcgttt
    24301 ttatgcatca ccagaagcga gagcgatggc accgcgtttg ttgctacatg cagagatgtt
    24361 gacgattacc catccggcgt atggcaatag tatgacgttt aaagcgccag cggattttta
    24421 atagtcacgc tggcgcagtt cggtaggcgg ataaggcgtt cacgccgaat ccggcaattg
    24481 tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca ggcctacagt ttaaaactta
    24541 tttaaaccct ttctgctgct ttatcagctc atatgcctgc tgaatttcct gcgctttctg
    24601 cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat cgggatggtg
    24661 ttcactcatc agcttacggt aggctcgttt aatggtggtc gcatcatccg tcggcttcac
    24721 acccagcaca ttacaggcat cttccagcgt cgggccacgc tgcgcttgct gccagttacc
    24781 accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa
    24841 aaactggtca aactgggcgc gggagatccc taattcttct gcaatgacat acagcaccgc
    24901 ccgttcattc gggtgcagtg aaccatcagc aaacgctgcc tgaatctgga tctccagaaa
    24961 catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca tcttttcgcg
    25021 cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc
    25081 gccatgaagg ttcattcggt ccatcagctg gctggcgata tgaatatcag cctccgtgac
    25141 gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc
    25201 ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt tatcaaacat
    25261 atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca gtaaggccac
    25321 ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac acacggatat
    25381 cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgca gtcaattgtg
    25441 ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc taacaccact
    25501 gacgctattc ggacaggatt aacactagcg tagagatgac gagtacgtta gtctctgacc
    25561 gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta
    25621 tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag ggactggcag
    25681 ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct ctggtacagg
    25741 gcgataccaa tgacttaccc gtgactatca atgctgacca cgcgaaaggg gactacccgg
    25801 atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt ctgcaggccg
    25861 acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta cgaaccgttg
    25921 atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg ccgaaaggct
    25981 gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag atggtgggtc
    26041 gccagggccg cggtaaagcg gacctgatga aacaacgtgg tgaaaaccgc tataccattc
    26101 tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc gtggtaggta
    26161 gcgaaattat tcatgaccgc gaagaacaag ttgcggagat ctggaacgcc cgctttaagg
    26221 tgggtccagt accgatcttt tatagcccct atttgcagtt gccggtgggt gacaaacgtc
    26281 gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt gagttctacc
    26341 tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg cattatatgc
    26401 atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccag gcgggcgctg
    26461 gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa cacccgaacg
    26521 atgacagttc acgtcgttgg ttgttctact ggcaacactc cggggtcatg gatcaagtgt
    26581 ggcgtttcaa cgtagactac accaaggtca gcgatcctag ctacttcaat gatttcgata
    26641 acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt ggctatgcgg
    26701 tgcaaaactt caatgccacc gtttcaacca agcagttcca ggtctttagc gagcagaaca
    26761 ccagtagcta ctcggcagag ccgcagttag acgttaacta ctaccagaat gatgttggtc
    26821 cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga gacgacatgc
    26881 ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct aataactggg
    26941 gcagcatcaa taccgaagcg aagttgctgg caacccatta tcagcaaacc aatcttgact
    27001 ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgtt atgccgcaat
    27061 tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct ccgggttata
    27121 cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca
    27181 tctataacta cgactcgtct ctactgcaat ctgactactc tggcctgttc cgggaccgga
    27241 cttacggcgg tcttgaccgt attgcctccg ctaaccaggt gacgaccggt gtcacatctc
    27301 gcatatatga tgatgctgcc gttgaacatt ttaatatttc cgttggtcaa atctactatt
    27361 tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac aaaacgggct
    27421 cactggtgtg ggcaggcgat acttactggc gtatctccga gcgttgggga ttgcgtggcg
    27481 ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc attgaatacc
    27541 gtcgggatga agaccgtctg gtacagctga attaccgtta cgccagcccg gaatatattc
    27601 aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt atttcgcagg
    27661 taggtgctgt cgccagctgg ccaattgccg atcgttggtc cattgttggg gcctactact
    27721 acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac agctcctgct
    27781 gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac gataaacaac
    27841 atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg agctccaact
    27901 acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa aacactttgt
    27961 gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa aagtatgaag
    28021 aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt cgctgccccc
    28081 caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt
    28141 gatggattaa tgcagtcggt aaaactgaac gctgcgcagg caaggcagca gcttcctgat
    28201 gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat catcctgcag
    28261 atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc gattgctaac
    28321 atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc ttacgatggt
    28381 ctgaactaca acacctatcg taaccagatc cgcaaagaga tgattatctc tgaagtgcgt
    28441 aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg aagtcgaatc cctggcgcag
    28501 caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat cctgatcccg
    28561 ctgccggaaa acccgacttc tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt
    28621 gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca ttctgccgac
    28681 cagcaggcgc tgaacggcgg ccagatgggc tggggccgta ttcaggagtt gccgggtatc
    28741 ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc
    28801 gttggcttcc atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg
    28861 accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac tgacgaacag
    28921 gcccgtgtga aactggaaca gattgctgcc gatatcaaga gtggtaaaac gacttttgct
    28981 gccgcagcga aagagttctc tcaggatcca ggctctgcta accagggcgg tgatctcggc
    29041 tgggctacac cagatatttt cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa
    29101 ggtcaaatga gtgcaccggt tcactcttca ttcggctggc atttaatcga actgctggat
    29161 acccgtaatg tcgataaaac cgacgctgcg cagaaggatc gtgcataccg catgctgatg
    29221 aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgcgc cagcgcctac
    29281 gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact cccggcgagc
    29341 ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac
    29401 tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc ggtttgccgc
    29461 tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactgcg ggcacattaa
    29521 cgctacttcc tgtcgcgcta cgcgaatctg tcactgcggg gcagttagcg attgaaaatg
    29581 ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg
    29641 ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaggt attccgttta
    29701 ctggtcatac cgagtttttc gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg
    29761 cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc gatatcgcag
    29821 atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac gatttgcgta
    29881 caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg
    29941 aaggcggtca tatgggtacg gaagagatag acaccattat tccggtgctc gacgagctgc
    30001 gggcgcaggg gatgatactc aacgggccgc tgcctgccga taccctgttt cagccgaaat
    30061 atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt cccgtgctaa
    30121 aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt attcgcacat
    30181 cagtggacca cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt
    30241 ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga ataatcgagt
    30301 ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt
    30361 gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg
    30421 ccccggtctg gcggcattga ccgagccggt cggcgaacgt ctggaccagc tgacggtcat
    30481 cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag gcccgaaact
    30541 gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg agaaaatggg
    30601 tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt tgatgttcca
    30661 tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa aagaggtggt
    30721 gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg tcatggcgca
    30781 atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta caccaccacc
    30841 caaagtggat tccgccgtcg tgcgcctggt tcctcatgca acgatgcctc acccggttaa
    30901 agatgttcgt gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat
    30961 tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc
    31021 ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga actatctggc
    31081 ggagaacgcg cctttgcagg agagttaacg atgatcaatt cgccccgagt gtgtattcag
    31141 gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta cgtttttgct
    31201 tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg gcgttactgg
    31261 ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc
    31321 cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat cattgaaacc
    31381 ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt ccctttcagc
    31441 atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa gaatatggcg
    31501 acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt gctgcataaa
    31561 gtagaattta cccctgggaa agataccctc tggctgacgg gcgatctggt cgcgcgcggc
    31621 ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt acgtctggtg
    31681 ctgggcaatc acgatctgca tctgctggcg gtatttgccg ggatcagccg caataaaccg
    31741 aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct taactggctg
    31801 cgtcgccagc ctttgctgca aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg
    31861 atcacgccgc agtgggatct acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg
    31921 ctatcgagtg actcctatcc cttctttctc gacgctatgt acggcgatat gccaaataac
    31981 tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc ctttacccgt
    32041 atgcgttttt gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc gccggaagag
    32101 gcccctgccc cactgaaacc gtggtttgcg attcctggcc ctgtcgctga agaatacagc
    32161 atcgcctttg gtcactgggc atcgctggag ggcaaaggta cgccggaagg tatatacgcg
    32221 ctggataccg gctgctgctg gggtggtaca ttaacctgcc tgcgctggga agataaacag
    32281 tattttgtcc agccgtcgaa ccggcataag gatttgggcg aagcggcggc gtcttaaaca
    32341 cagcctgata taggaaggcc ggataagacg cgaccggcgt cgcatccggc actagccgta
    32401 aattctatac aaaattaccg ccgctccaga atctcaaagc aatagctgtg agagttctgc
    32461 gcatcagcat cgtggaattc gctgaatacc gattcccagt catccggctc gtaatccggg
    32521 aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt ctgcgctttt
    32581 ggcaggaact gctcataaac gcgaccgccg ccaatcacca tgatttctgg tacgtcacca
    32641 cacgccgcga tggcttcatc caccgacttc acccacgtta cgcgatcgtc cgtacccggt
    32701 tgactgctga ggataatatt tttgcgtcct ggcaacggac gaccgattga ttcccaggta
    32761 tggcggccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc gagatcggca
    32821 ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc taacgccgca
    32881 atcagactga tcattgagat ttcccgataa aaaaaattgt cgccactata cgtaaagcgt
    32941 aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa ttttatattc tgctggcgag
    33001 tccacgctct ctccctggac tcgccgcatt acaatgaaac aaaaacaaac agttagctgt
    33061 aaagtgtgat ttatgtcact ctttattagg atgagggttt cgtttccggt tcatccgcca
    33121 tgttgccggt atgtttacct tcttcggttc cctgccagcc atgtcgttga attaatgaca
    33181 gatgttcgcg gtcctcggta atgatctcac ttaacatcgc gctggtgcgt ttatagaccg
    33241 ccgcgcgggc tttggtgtcg ttctcaacca ttgccatctc ttccaccatc tgaatattaa
    33301 agcggcggaa cacatcggca cgttctcgcg cttcatacgg ccccagacct aaactttcca
    33361 gtgccagacg cccggttttc agcgcacctt cgaaggtttc acgctccggc ttttcaacgc
    33421 ctgcctgacg caaacgaatg tagtggtcga catcgcgggc gcgggcaata atctgcaaat
    33481 gcgggaagtg ttctttcacc atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg
    33541 cgttaatcag cacttccgct ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg
    33601 catcgccata aaacactttc ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt
    33661 gatcgagcac caccattttc accccgctgg agagcagtaa acgtccggtg atctgcccaa
    33721 aacgaccgaa tccggcaatg attacccgcg gctgttcttc gtcgatctca tcggcttcac
    33781 gcgcctcttc agtagaagat tgctcaaggc gattgaggat caccagcaga atcggcgttg
    33841 ccgccatcga cagcgccacc gccagggtca gcgatttcgc ccactccggc tccagcacat
    33901 tcgccatctg cgccgcgcca aataccacaa aggcaaactc actgccctgc cctaacaaca
    33961 ccgcaaacca acgacgctgt ttatttggca cttgcaacgg tcgggcaatc agccacagca
    34021 tggcgatttt gatgatgagg aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa
    34081 gcagcgtgcc aaagtctatc gacatgccaa caccgatgaa aaacagcccc aacagcaaac
    34141 ctttaaaggg ttcgatatcg ctctccagcg catgacggta ttccgagctt gccagcagta
    34201 cgcccgccag aaacgcgccc atcgccatcg acaagccgac ctcttccagc agcaaaccaa
    34261 agccaaacac gaggaataac gccacggcac taaacacttc ccgcaagcca gagcgggcta
    34321 caaaacgcag cgccggacgc gtgacatagc gccccagcaa taccaccagc accagcgcac
    34381 ccgccacttt taacgccgag agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg
    34441 ccagtagcgg aatcatcgcc accagcggga tcgccgcgat atcctggaac agcagcaccg
    34501 caaaggcact gcgacccatt tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg
    34561 caatcgccgt agaggagagc gccagcgtca tgccgatcaa ttccgcgacc tgccagcgca
    34621 gcccaagtaa catgcagaac agccccagca ggccgccgca aatcaccatc tgcaatgcgc
    34681 caccgccgaa cactgccgca cgcagcttcc acagcctttg tggatcgagt tcgaggccga
    34741 taataaacag catcagcacc accccaatct cggcaaagtg cagaatagat tcggcatcgg
    34801 tcaccagtcg cagcccccac gggccaataa tgcagccggc gatcaggtag ccaagtaccg
    34861 atcccagacc aagacgtacc gcaatgggta caatcagcgc tgccgaaccg agataaatca
    34921 gcgcctgaat cagcgtatgg ctatccatga tgggcctcct gccattccag cagacgttgc
    34981 ttatagtgac gcgcctgccc ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg
    35041 gcaaacggtg gcagccagtt cagcccgcag tagattgccg tcgcctgtag cggctgcgac
    35101 agcacatcaa agcccggatg cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc
    35161 accgcccaca gcaaatgttt gccatgcagc gccttgccgc cgtgaccgta agcccagccg
    35221 tgcgacaaaa ctttatcgat ccaaagttta agaagcggag gaatgctgta ccactgcatc
    35281 ggatgctgcc agacgatcag atcggcgcga gacagcgcct cctgctcggc ggcaatatcg
    35341 atattgaagt caggatagag ttgataaaga gagcgaattt cgacgccttc cagcgtcctt
    35401 gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg gatacggatg cgcataaatt
    35461 ataagaatca taggtagccc gcgtgattac gcctttgttt tcccacaaag tgtagtcata
    35521 tattcacgcg gcttatagtc aataatattg attcagaaaa gagagaaatc tgaacaattt
    35581 accccattct ctgcccgcgg atcgtcatat tgctggtttg cgccaatgac atccctcgat
    35641 gtctccgggg caaacgctac ggaaatcaac aagccaaaca gcgagatacc cgcccccatt
    35701 agcatcgtgt tactgatacc gtaattattg ataaagatcg gcagtgccca ggtcgaaaca
    35761 atggtgccaa tacggcttaa ggacataatc acgcccacgg cagaggcgcg gatatctgtc
    35821 gggaagagtt cattaggata gagccactgc aaattacccg gcccgccaga gaaaaaggca
    35881 tacaccgcaa aggccatcac taccagccag atccccatat ccgggatcag ccccaaaacc
    35941 gccagctcca gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg tccggcagtg
    36001 tttaaccaca gcatcggcgg aatacagccg agcataaaga acaggctaat caccacgttc
    36061 cccagcgccg cgtttttgcc aacacccaat cccaacaaac caacgatttg cgggccaaag
    36121 gtgtaaatgg cgaacattgg gatcacctgg caggtccaga tggcggcaac aaacagaaca
    36181 aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg ctgctcttca
    36241 tcg
//
LOCUS       DAJVOY010000019        35881 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVOY010000019 DAJVOY010000000
VERSION     DAJVOY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.019"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            98..445
                     /gene="grxD"
                     /locus_tag="OQL90_000851"
     CDS             98..445
                     /gene="grxD"
                     /locus_tag="OQL90_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS1367718.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(496..4903)
                     /locus_tag="OQL90_000852"
                     /pseudo
     CDS             complement(496..4903)
                     /locus_tag="OQL90_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4996..5643)
                     /gene="rnt"
                     /locus_tag="OQL90_000853"
     CDS             complement(4996..5643)
                     /gene="rnt"
                     /locus_tag="OQL90_000853"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1367719.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRSAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5746..6153)
                     /gene="gloA"
                     /locus_tag="OQL90_000854"
     CDS             complement(5746..6153)
                     /gene="gloA"
                     /locus_tag="OQL90_000854"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1367720.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6234..7331)
                     /gene="nemA"
                     /locus_tag="OQL90_000855"
                     /pseudo
     CDS             complement(6234..7331)
                     /gene="nemA"
                     /locus_tag="OQL90_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(7368..7967)
                     /gene="nemR"
                     /locus_tag="OQL90_000856"
     CDS             complement(7368..7967)
                     /gene="nemR"
                     /locus_tag="OQL90_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1367721.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8070..8309
                     /locus_tag="OQL90_000857"
     CDS             8070..8309
                     /locus_tag="OQL90_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1367722.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8358..9254
                     /locus_tag="OQL90_000858"
     CDS             8358..9254
                     /locus_tag="OQL90_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1367723.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9335..9856
                     /gene="sodC"
                     /locus_tag="OQL90_000859"
     CDS             9335..9856
                     /gene="sodC"
                     /locus_tag="OQL90_000859"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1367724.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9857..11868)
                     /locus_tag="OQL90_000860"
                     /pseudo
     CDS             complement(9857..11868)
                     /locus_tag="OQL90_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994342.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11868..12725)
                     /locus_tag="OQL90_000861"
     CDS             complement(11868..12725)
                     /locus_tag="OQL90_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1367725.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12791..12964)
                     /locus_tag="OQL90_000862"
     CDS             complement(12791..12964)
                     /locus_tag="OQL90_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1367726.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            13165..13599
                     /gene="slyA"
                     /locus_tag="OQL90_000863"
     CDS             13165..13599
                     /gene="slyA"
                     /locus_tag="OQL90_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1367727.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13646..14113)
                     /gene="slyB"
                     /locus_tag="OQL90_000864"
     CDS             complement(13646..14113)
                     /gene="slyB"
                     /locus_tag="OQL90_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1367728.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14387..15496
                     /gene="anmK"
                     /locus_tag="OQL90_000865"
     CDS             14387..15496
                     /gene="anmK"
                     /locus_tag="OQL90_000865"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1367729.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VIRPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15594..15923
                     /gene="mliC"
                     /locus_tag="OQL90_000866"
     CDS             15594..15923
                     /gene="mliC"
                     /locus_tag="OQL90_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS1367730.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15982..16638
                     /gene="pdxH"
                     /locus_tag="OQL90_000867"
     CDS             15982..16638
                     /gene="pdxH"
                     /locus_tag="OQL90_000867"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1367731.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16767..18041
                     /gene="tyrS"
                     /locus_tag="OQL90_000868"
     CDS             16767..18041
                     /gene="tyrS"
                     /locus_tag="OQL90_000868"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1367732.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18100..18963
                     /gene="pdxY"
                     /locus_tag="OQL90_000869"
     CDS             18100..18963
                     /gene="pdxY"
                     /locus_tag="OQL90_000869"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1367733.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(19007..19612)
                     /gene="gstA"
                     /locus_tag="OQL90_000870"
     CDS             complement(19007..19612)
                     /gene="gstA"
                     /locus_tag="OQL90_000870"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1367734.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19718..21220)
                     /gene="dtpA"
                     /locus_tag="OQL90_000871"
     CDS             complement(19718..21220)
                     /gene="dtpA"
                     /locus_tag="OQL90_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1367735.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21831..22466)
                     /gene="nth"
                     /locus_tag="OQL90_000872"
     CDS             complement(21831..22466)
                     /gene="nth"
                     /locus_tag="OQL90_000872"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1367736.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22466..23161)
                     /gene="rsxE"
                     /locus_tag="OQL90_000873"
     CDS             complement(22466..23161)
                     /gene="rsxE"
                     /locus_tag="OQL90_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1367737.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(23165..23785)
                     /gene="rsxG"
                     /locus_tag="OQL90_000874"
     CDS             complement(23165..23785)
                     /gene="rsxG"
                     /locus_tag="OQL90_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1367738.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23789..24847)
                     /gene="rsxD"
                     /locus_tag="OQL90_000875"
     CDS             complement(23789..24847)
                     /gene="rsxD"
                     /locus_tag="OQL90_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1367739.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24848..27070)
                     /gene="rsxC"
                     /locus_tag="OQL90_000876"
     CDS             complement(24848..27070)
                     /gene="rsxC"
                     /locus_tag="OQL90_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1367740.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(27063..27641)
                     /gene="rsxB"
                     /locus_tag="OQL90_000877"
     CDS             complement(27063..27641)
                     /gene="rsxB"
                     /locus_tag="OQL90_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1367741.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVELHA"
     gene            complement(27641..28222)
                     /gene="rsxA"
                     /locus_tag="OQL90_000878"
     CDS             complement(27641..28222)
                     /gene="rsxA"
                     /locus_tag="OQL90_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1367742.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(28299..28739)
                     /locus_tag="OQL90_000879"
     CDS             complement(28299..28739)
                     /locus_tag="OQL90_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1367743.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(28825..29040)
                     /gene="ydgT"
                     /locus_tag="OQL90_000880"
     CDS             complement(28825..29040)
                     /gene="ydgT"
                     /locus_tag="OQL90_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1367744.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(29313..29438)
                     /gene="blr"
                     /locus_tag="OQL90_000881"
     CDS             complement(29313..29438)
                     /gene="blr"
                     /locus_tag="OQL90_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1367745.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            29651..30691
                     /locus_tag="OQL90_000882"
     CDS             29651..30691
                     /locus_tag="OQL90_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1367746.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(30726..31727)
                     /gene="add"
                     /locus_tag="OQL90_000883"
     CDS             complement(30726..31727)
                     /gene="add"
                     /locus_tag="OQL90_000883"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1367747.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31830..33002)
                     /gene="malY"
                     /locus_tag="OQL90_000884"
     CDS             complement(31830..33002)
                     /gene="malY"
                     /locus_tag="OQL90_000884"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1367748.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(33012..34604)
                     /gene="malX"
                     /locus_tag="OQL90_000885"
     CDS             complement(33012..34604)
                     /gene="malX"
                     /locus_tag="OQL90_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1367749.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            34779..35807
                     /gene="malI"
                     /locus_tag="OQL90_000886"
     CDS             34779..35807
                     /gene="malI"
                     /locus_tag="OQL90_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1367750.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         8988 a         9227 c         9339 g         8327 t
ORIGIN      
        1 aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg cactgacccg ctacaatgtc
       61 agattactgc cgtaaagaag tttgaggaag caagacaatg agcaccacta tcgaaaaaat
      121 ccaacgccag attgctgaaa acccgatcct gctgtacatg aaaggttcac cgaaactgcc
      181 gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg aacgttttgc
      241 ctatgttgat attctgcaga atccggacat tcgtgcggaa ctgccgaaat atgctaactg
      301 gccgaccttc ccgcaactgt gggttgacgg cgagctggtc ggcggttgtg atatcgtgat
      361 cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa gaaactgccg ctaaatacaa
      421 gtctgaagag ccggacgcgg aataatccgt cctgcaatac tgacggatgc gaacgggcat
      481 ccgtcagtcc tttctctatc cccaatccag cccttgtggc gaacttgaaa atcccgcctc
      541 acgcagcagc gtaaacatcg gcgtttgccg gaccggtaga tcattcactt ctgttagcgt
      601 aaagcgcagc cgtggttcgc gacgcagtgc ggtagtcagc gcgtggaaaa cctctggggc
      661 ttttcctgcc agaccagcat ttttttgcca ccttgcgcca gatagagtaa caatttgccg
      721 ctagaaacga ccaccagcgc acccgcccga cgcgttggaa ccagcgttgc cggatgtgca
      781 ggccagggca gaagatttcc ccacacatta gccggatcgt tggcagagag cgccactggt
      841 gtatagtggc gcgtttgcgt ggcttgtgtc gccagatcgc gccatcggtc aatagtcaga
      901 cgttcagcga attgcgcgcc acccagacct tctacaaaac gacctcgcat aattcgcccg
      961 gagtcttcca tacttcgaca aagcgtttgc atcgatggaa acccgccagg gatattttcg
     1021 gctatcaccg cctgacgact gatgatgccg tagcggtcga gcatattttc cgccagcgcc
     1081 agcatccttt cggtatcgtt tagtggctcc acctgcaata acgaccagcg tccagccaga
     1141 tttggtgtgt tgtaagatac cagcggcgag acgggttgcg catagacagg acgtccacga
     1201 cgagcccggt gactgcggcg agttgaggtg cgtgcgttgg aactgctgcg ggtgagggcg
     1261 cgtaacggtg cccaaatgtc gctggtgatg acgccttgcc agaccagcgc ccataacgcc
     1321 tcttgcaggg cagagagatc aaccgattcg ccgattttgt cgcgtatccg ttggctgatt
     1381 tgttgtgcaa accaggctcc tccgtcagcc agaacagcga ctatcgcttg ttgcagcgcc
     1441 gaacgattcg cctgatccgc ttcggcagga gtgaacgatt ctgcagcata ttcctgtaga
     1501 tgcagtgcca ccaggccgtc atcttcaccc agtttttttt gccccgacca gataaccgca
     1561 ccggttgcca gtaattcatc gagcatttct gacgaatagt cgcgtacgcg ggcaggcagg
     1621 atctggcttt cccagagtga ggcgggtaaa ccgactccgg caagctgttc gatcacccgc
     1681 atcacgccat ctacgccctc ataaacgcct ggcgatgttg aggcaaagag cgccgggcta
     1741 ccatcggtgg cgggtaatac gccctgacgt tccagcagca atcgcgcata ggtcgtggct
     1801 gcaacgggac gcgtcgcttc tctggcggct tgcagcgagc gcaaacgcag acgacgaaat
     1861 acttcatcgc tcacccagat gtcttgttgc agattcatca ccagaccctg ttcacgcagt
     1921 tgctgaagct gctcttcgac aatggcaata ccgagactaa actcatgagc cagttgttca
     1981 gccgtgacca gagcatgagc ccggagatag cgcagaaaga ggtcgcgaag cgggtaactg
     2041 actctatgta aataaatctc tggcaatgac tctggtagtc gtacgccgag ggcatcacgc
     2101 agcctggcgg catcatccat acaggcaaga cgctcctgtc cgctaatcat cgctgggaag
     2161 attcgtttta ctgcaagaag attttccaga tacgacgcaa cctcttcact gctgcctgta
     2221 tgccgttgcg ccaggtcttc aacggtcatt ggccccagtt cgcgcagcag gtcgaacaat
     2281 ccttcttcac ctttcgctct tctgccagga gccagtcgtt gcaactcttc ttccacctgg
     2341 cgaatgacct gcgggtcgag taattccccc ggatcgacct gtccgagtag attgcgcagt
     2401 aactcgctgt ccagcgacag tacggatgcc cggcgctctg ccagcggggc gtcgctctgg
     2461 tacataaatt ccgcgacata gccgaacaat aaacttgtgg cgaaaggcga gggcgtagtg
     2521 gtcgttacat cggatatttg aatttcgcca ccgttcaggc gacgcatcaa acgttccagt
     2581 gcgggaagat cataaacatc ttgcagacat tcgcgtagga tttcgagaat gaccggaaaa
     2641 tctggatatc cctgagcgat ttccagcaac tgactggcgc gcagccgttg ttgccaaagc
     2701 ggggtgcgat ggcccggagt gcgccccggc attaataatg cccgcgcggc gcattcgcga
     2761 aaacgggcgg cgaaaagtgc cgagctgcct accgcctcgc ggacaatttg cagcaacttt
     2821 tctggttcaa acaaaaaaat cgcggcgtcg ggcaatttac catcagtgtc aggaatgcgc
     2881 gcaacaatgc cgtcatcact ggcgaccaca gacgcgtcag cgcccagggt tcatgcaccc
     2941 gtcttccata gggagagtgc aaaataatac gccagtcgcc aatttcatca cggcaccgtt
     3001 cgaggaccag atggcgactc cccggaacga tgcccgtcgc gttgcgttgc tcgtcaatca
     3061 acccctgaat attggcgttc gtattttctt ctgccagcca cgggggaata gtcccggaaa
     3121 agaacgcgcc atccgccagt aaatgaagaa aatcgccgat catctcgcct aattcagccg
     3181 gacgtccgtt accttcacca cgccagaagg ggagccgggc agaacgaccc ggagcaggag
     3241 tcacaatcac ctgatcgcgg gtgatttgct ggatccgcca tgaggtagcg ccgagagtga
     3301 taatgtcgtt cacccgcgac tcatatacca tctcctcatc cagtttaccc acccgccgcg
     3361 aaccggcctt ttcttcacct tcgggtaata acacgctata catgccacga tccggaatgg
     3421 tgccgccgct ggtaaccgcc aacaattgag cgccaggtcg gacggtcaat atcccggtct
     3481 ccctgttcca gaccagtttg gggcgaaaag cagaaaaatc gccagaggga tagcgcccgg
     3541 aaagcatatc cagcgtggcg tcaaaaacac gtcttggcag atctttccac ggtgcggcac
     3601 ggcgtacgcg ggagtaccat tcgtctacct gtaatgcctc catcgccgcg gcggcaacgg
     3661 tttgctgcgc aaggacgtcg agtggattat gcggcggtgt caggttttcc agcctgcctg
     3721 cgaacataca ctctacaatg actgcggaat cgactaagtc acgccgggta cgagggaaaa
     3781 acagcccttt agatacaccg ccaacctgat gtccggcgcg accaatgcgt tgtaacccac
     3841 tggcaacaga aagcggcgtt gccacctgaa tcaccagatc cactgcgccc atatcaatcc
     3901 ccagttcgag actggaggtt gcgaccacgc agcgtaactc acccgatttc agcgcctgct
     3961 cggtgattgc tcgttgttct ttagagacgg agccgtggtg tgagcgggca ataaaaacgt
     4021 cgctactttg tacacggtta gaggttgcgc cggaggtcga ctcgaaatgg gccgcatcaa
     4081 cggcgataga cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat cgtgccgtca
     4141 gtttttccgc cagcccacgt gaattagtaa agacaatggt cgagcgatgg cgcaacactt
     4201 catcaaggat acccgtttca atatatggcc agatggagcc ttcccggccg gcatggctgt
     4261 cttcgccggt gctgctggcg accgatgaga catcatccat attggcgacc ggtacgacaa
     4321 ttcgtatctg cggatggcgc attgcgggcg ggttgactac cgtaaccggg cgatcgccac
     4381 caagaaaggc tgccacatcg ctggctgagc gcacagtggc agaaaggcca attcgctgtg
     4441 ctgaggtgtg gagcagcgca tcgagccgct ccagacttaa cgccagatgc gcaccacgtt
     4501 tactgcccgc taccgcgtgg acttcatcaa taattaccgt ttcgccgcgc cgccgccgtt
     4561 catcggcaat acccttcaac gggagctgca agttgcgctg aacgtcggtg cccagagctt
     4621 ttatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg cgggtatctt
     4681 cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc gttttcccgg
     4741 agccggtcgg tgcaatcacc agcgcatgtt cgcttcgcgc cgccacatgc caggtttgcg
     4801 actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa
     4861 acacgtccgg caggagcgat gaagggtctg gattatctgc cattaacgct attccccgtg
     4921 aatatcatgc aggatgcgtg gaatagcctc actatagatg cctgaatcat tgcatgtcat
     4981 caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc ccagacgttt
     5041 ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt acagcgcgga
     5101 gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg acaatacggt
     5161 ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg
     5221 gttacgtttc agtgaggcgc gttctgcggc ggctatcata aagctgtgat caaaattggc
     5281 attgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatacctt tacgtacaac
     5341 tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ctacgatcgg gatcgttcgg
     5401 gtcaatgccg ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa atggttcgac
     5461 gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt tcagggtgat
     5521 ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt caacatcgat
     5581 cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag cgttatcgga
     5641 catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata ttacttgtcg
     5701 atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt tgcccagacc
     5761 gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat cttccataaa
     5821 cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt tacccccgtt
     5881 ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga tgtgaccata
     5941 agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc
     6001 ttcggtttcc gggccgtagc caacaaacgc cagtgagtat ttgtattccg ggttttcgct
     6061 ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc gttgcaaatc
     6121 gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat ggctaacaga
     6181 ttcaaaatgt tgttttagta tagcggcggc tttacgccgc tcgcaatgtt ggattacaac
     6241 gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga aactttcggc acgctgtggg
     6301 ttaagctcag ctttgcgctg caagcgggcg accagatccg ggttcgcaat ccagtcacga
     6361 ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcagcttt ttctactgtg
     6421 tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc gcggaacgca
     6481 tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata agcaatgccg
     6541 cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg gccgttatcc
     6601 gtgttctgga aagtaccgat tggcgaaatg cgaatgccaa tgcgatcggc accccattct
     6661 tcaatcccgg catcgaccac ttccagtacc agacgtgcgc gattttccac gctgccgccg
     6721 tactgatcgg tacgatggtt tgaagaagga gaaaggaact gatgcagcaa ataaccgtga
     6781 gcagagagga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat ggcctgacgg
     6841 aaatcattga cgatacctgg aatctcttct agttcaagcg cacgcggcat ggatgtttca
     6901 acacggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc gctaagtgct
     6961 gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat gcgtccggtg
     7021 tgccacagct acacggccat atgaccattt tcagcatgaa cgccagcggt gatttttttc
     7081 catgcggcaa tttgctccgg actatggatg ccaggcgcac ctgcatatcc ttttacctgg
     7141 gcagaaattt gcgtggcttc actaataatc aaaccggcac tggcacgttg gcgatagtat
     7201 tccgccatca acggggtagg aatgtcaccc ggttcaatac tgcgcagacg cgtcagcggt
     7261 gccataaaaa tacggtttgc cgccgtgatc gcgcccactt tcagtgggga atacagtttt
     7321 tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta aacggcaggc
     7381 gtcgcaataa tgttttttac atgggccagc gcgttttcca gtggctcgaa actgcgcgaa
     7441 attttggcct gcagattcgc acccagccat agtgcgtaaa gcacttgtgc ctgttgtagc
     7501 ggttcgccac aaaaggttaa acaatggttc tcacggccat tttccagcgc ctgagagagc
     7561 agggcgatca cgccgcgagc acctttatcc atcgcgctgc gcatatcttc tgacagatcg
     7621 cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg ttggcaaaac
     7681 tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc ttcgccggat
     7741 tgcagcaact cagtcagtcg ctggtgatat gcggtgtaat gacgctcaag catggcaacg
     7801 ccaaacgctt ctttagagcg aaagtagtga tagaaggacc ctttcggcac ttcagcggtt
     7861 tttagtaatt cgcttagccc catcccggtg aatccacgtt gcaggcaaag ttgctcgccc
     7921 gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg ttggagtgta
     7981 gtagaccagt cggtctagtg caagcaaagt tgctactttg gacattaaca gcttcaataa
     8041 tggtaataat cttatctgag gagggcgaag tggcggagca attagagttc tttcccgtcc
     8101 agagcccgtg ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc ggctgttttc
     8161 gtagtcggga tgagcgtttt aactggaata aaatgagcga tggcgaaaaa caagaggtgc
     8221 tgcgcctttg tcggcagcga ttaatgcgca aattacgcgc aaataagcca gcgtcatcag
     8281 acgaaccgga gcaaccatca ctcttttgaa cctaatcttg cgtatactcc tctcatatct
     8341 gataagagga agcgattatg gttcagcgta ttactattgc gccgcaaggc ccggagtttt
     8401 cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc cgccagctgg
     8461 tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat gctgatattt
     8521 atggtggcta tcagtgcgaa gcggcgtttg gcgaggcact gaaactggca cctcacctgc
     8581 gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt gaagaaaacg
     8641 tcattggtca ttacatcact gaccgcgatc acatcattaa gagcgccgaa cagtcgctaa
     8701 ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac ccgttaatgg
     8761 atgccgatga agtggcggac gcgttcaaac atctgcatca gagcggcaaa gtgcgtcatt
     8821 ttggcgtatc gaactttacg cctgcgcaat ttgccctgtt gcaatcacgt ctgccgttta
     8881 cccttgccac taatcaggtg gaaatatccc cggtgcatca gccgttactg ctggatggca
     8941 cgctcgacca actacaacaa ctgcgtgttc gtccgatggc gtggtcctgc cttggtggtg
     9001 gtcgtctgtt taatgatgat tatttccagc cgctgcgtga tgaactggct gtggtggcag
     9061 aggagttaaa cgcgggctcg attgaacagg tggtttacgc ctgggtatta cgtttaccat
     9121 cgcagccgct gccaattatc ggttcaggta aaattgagcg cgtacgggca gctgtcgaag
     9181 cagaaacact gaaaatgacc cgtcaacaat ggtttcgtat tcgtaaagcg gcactggggt
     9241 acgacgtacc gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc actggtttac
     9301 acttattcag gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc tggctattct
     9361 ggcgctggtt gttgcaaccg gcgcacaagc tgccagtgaa aaagtcgaga tgaacctcgt
     9421 cacgtcgcaa ggggtagggc agtcaattgg tagcgtcacc attactgaaa ccgataaagg
     9481 tctggagttt tcgcccgatc tgaaagcatt accccccggt gaacatggct tccatattca
     9541 tgccaaagga agctgccagc cagccaccaa agatggcaaa gccagcgccg cggaatccgc
     9601 aggcgggcat cttgatccac aaaataccgg taaacatgaa gggccagaag gtgccgggca
     9661 tttaggcgat ctgcctgcac tggtcgtcaa taatgacggc aaagctaccg atgccgtcat
     9721 cacgcctcgt ctgaaatcac tggatgaaat caaagacaaa gcgctgatgg tccacgttgg
     9781 cggcgataat atgtccgatc aacctaaacc gctgggcggt ggcggtgaac gctatgcctg
     9841 tggtgtaatt aagtaattaa gaggcctgcg taccttgcgg tggtgcttgc tcaagttgcg
     9901 aaagcgagca gtacagccgc cagacaattg ccgccagctc acgggcggca ggttgatgat
     9961 gacgggcaag gctgtcgcaa atccgctgta attcttcaag tgtggccgcc agtgattttt
    10021 gctgaacgcc acgctcactc atcactcccc gcaacagtga aatacagtta tcccgtactc
    10081 gcgataacgg atcggaacgc gattcccagt cgcgcaattg ccagacaaca tgagaacagt
    10141 tcagcagcac tacaccccag cgtaataacc aacggcgagc cagcgcatcc tggctgttac
    10201 tcaactgact gacgtgatga taagtgagcg attcaaattc gctttcactc agtgttggat
    10261 ggcggcttag ctgatcgaca aaatcccggc gcagcgcgcg aatatggcgg cggcttttac
    10321 gagcatccga tcctggacgc agaatggcga acgctagcca agccagcgca acgccaacga
    10381 tttttgccag attatcgtta agaaagtcag caaaatcata caccggggga ttagtgacag
    10441 cgataaacga acccataaaa acaatcagtt gcccccacag tgcggcaaat ttgggcatct
    10501 gcaatttaag aagctgcatt gtcgccagca gtggaaagag aaacagtaaa aattgccaca
    10561 gatcgctaat ctggaccatc aggccaaatt tgaccacaaa gctgaatagc gaaagtaaca
    10621 ccagcgtgcg catcagcagt gacaacgact taaacggtgc tgcgacggcg gagtagagta
    10681 cgcagctaat tgctgccagc gttaatgcat tggcaccggc atcctattgc gatgcaatac
    10741 tccatgcgcc aatcatcatc aacgtacaaa atgtacgcag cccgctccac atagcttcgg
    10801 cgttgtcggt atgacgagcc agagcggatg tccgtgcgag tctggcatga tcatctacac
    10861 cgctttgcag acgatgtaat tcctgactac tttgcagata aaggcggcaa aaataacgta
    10921 gtcgctgcca gaaggcgacg tgccggtagt cggcgacgtt ggtcgggcgt agcggggcga
    10981 taatacgtgc gacggtgtaa gcatctgttt gcgaactggc gagcgccgtc agcaattgtt
    11041 cgagaatttc tcgcgtggca cctggcggtg agggccagtt gagcaacata cgtcgcaggc
    11101 tggagatgac actggtcata cgtaattgct ggtggagtag cgcattaagg cgcgcgtttt
    11161 gctggcgaaa acgatagtgg ctccagaaag cctggatacg cagcaaattc atggtcagta
    11221 tctgcccaat ccccccctca tgtgctgcac gaatggcatc gtttgtttca ggctgccaga
    11281 gtaaactggc atgttccagt aatcgggcgt gcatgttttt caatgcggtt aaaagggctg
    11341 tagcatcgga actgctaggc aggatcatca tcatcatgcc gccgcacaaa ataccgacta
    11401 tcacctcgca aacgcgcgcc tgagcgatat cctacagctg gctggcctca gtaatattaa
    11461 ccatcggaaa ggcgatgatg gcagccgtgt agccgccagt tgaaatgcat acgcgacgtt
    11521 attcgtgaag tgcgcacagg cccaggtaca aaagccaagc cacgccgaca tgctcaatag
    11581 aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa tggctcccag
    11641 taaactgcca gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg tgggaaagct
    11701 aaccactgca gccgaggtca tcgcccaata gggttcatcc agattcaaat aataggcaac
    11761 cgtcagcgcc agacacatgg caatggtatt gcgtaacgca taacgccatt gcgccagcgt
    11821 ggccttgaac cagggcaaat tgcgcaagga ccatgacgat gcgttcatta ttgacctacg
    11881 gcaatgctac aggtggtgcc cgacaccagc gtgacatcgc ctggaacttt atccagcgca
    11941 aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt cacatccgga
    12001 ataaggcttg agtcactttc aacgctttga tcataaatcg cccgaccgat actggaaacg
    12061 tgaccccgta acgttttgtt atcactatag agggtaattt gtgctggtgc accttcgcgg
    12121 atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg gctgtcgaca
    12181 agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt gaggttagtc
    12241 acccaaccac tcaccggggc gcgaatttcc gtttgcgcca gttgccattg tgtctgtttc
    12301 agcgtggcct gtgcggcatt aacactggcc tgcatcgctt taacattgag gttagcggta
    12361 tcgagttctt cggcagaaat aaaattttgt gagagatgac gacgacgatt cgcttcgtta
    12421 ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcgtt cagctcggcg
    12481 atctgaaagg gcgttttatc gatcgtgagc agaagatccc ccgcgttaac cagttggttg
    12541 tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga aaccttttct
    12601 gcgcgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca cagccaccat
    12661 ccggcaagaa ccgcaactat cgctacaatg attattgaga aatacttaat tgttttaatt
    12721 gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc gcaaacagcg
    12781 ataaatccat ttacaaggga tgccagatgt caccggcgta gatccagcca cgaagcaggc
    12841 ggtgtacgac aagccagatg acgaatccaa acgcgaaggc tttgaaaaac ggaggaaaat
    12901 agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg agcataaact
    12961 tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa tattctttaa
    13021 gtgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca aatgcagcaa
    13081 tacatttgtt ttagcaataa aattgctgca cactattcta aaagccgcat aatatcttag
    13141 caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct ggcacggttg
    13201 gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt aacacaaacc
    13261 cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca aattcaactg
    13321 gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca actggaagaa
    13381 aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg tattaaactg
    13441 acggaaaagg cagagccact gatcagcgaa atggaagctg ttattaacaa aacccgcgcg
    13501 gaaatattac atggcatctc cgcagaggaa ctggagcaac tgattacgct catcgcaaaa
    13561 cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg cgtgtggtca
    13621 gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga ctgccattgc
    13681 tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc tgtaccacca
    13741 tgatggtatt accatcgtct ttacgaattt ccagctcgac accctgcgtt ttgttcattg
    13801 cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca gtagccagag
    13861 aacgcccggt tccgccacca acagtattcc ccaggaaacc accaagaaca gcaccgccaa
    13921 ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt acgttaacga
    13981 tggtgccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa acatcccctg
    14041 acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa acaaccaata
    14101 cgcgtttaat cattgaaaaa tctcctgttc accatgaaac gccacgcaag catccctcat
    14161 ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat atgagcaaat
    14221 cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt attgatttga
    14281 agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt
    14341 ttatgtttga gtggtgcgca aacgtcagat tgaaaaagga tgaactatga aatcgggccg
    14401 ctttattggc gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat
    14461 agatgaacac cgggtcgcac agctggcaag cttgagctgg ccgatcccgg tgtctctgaa
    14521 acaggctgta ctggatattt gccagggcca gcagcttaca ctttcgcagt ttggacagct
    14581 tgatactcaa ctcgggcgac tttttgctga tgcggtcaat gccttgctta aggaacaaaa
    14641 cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt caaaccgtct ggcatgaacc
    14701 gacgggcgtg gcaccacaca ctttacagat tggcgataac aatcaaattg tggcacgcac
    14761 cggaattacg gttgtcggtg attttcgccg tcgcgatatt gccttgggag gacaaggcgc
    14821 accgctggta cctgcgttcc atcatgcctt gctggctcac ccaaccgagc gacgaatggt
    14881 gctcaatatt ggcggcatcg ccaatctgtc actgctcatt cctgggcagc cggttggcgg
    14941 ctacgatacc ggtcctggta acatgctgat ggatgcctgg atctggcgtc aggccggtaa
    15001 accttacgat aaagatgccg agtgggcacg ggcgggtaaa gttattcgcc cactgctgca
    15061 aaatatgctc agcgacccat atttctcgca acctgcaccg aaaagcaccg gacgcgaata
    15121 ctttaactac ggctggctgg agcgccattt acgccatttt ccgggtgttg atccccgtga
    15181 tgttcaggcc acactggcag aactcaccgc cgtgaccatt tctgaacaag ttttgttgag
    15241 cggtggctgc gaacgattga tggtatgtgg tggaggtagt cgtaatccgc tactcatggc
    15301 gcgtctggcg gcattactgc caggcacaga agtcaccacc accgatgccg ttggcattag
    15361 tggcgatgac atggaagcat tggctttcgc ctggcttgcc tggcggacgc tggcgggatt
    15421 accaggaaat ctgccttccg tcactggcgc aagccaggag acggtactgg gggctatttt
    15481 ccctgctaac ccgtgatatc agagttaact gaatttttct taccgcgtct gccgttacac
    15541 ttggaacgtt agggagggcg tattgccctc cagaccagga aagtcttcgg gaaatgtcta
    15601 tgaaaaaact gttactgatc tgtttgccgg tgcttctcac cggctgtagc gcgttcaatc
    15661 aactggttga gcacatgcaa accgctacgc tggaatacca gtgtgatgaa aaaccgttga
    15721 cggtcaaact gaataatccg cgccaggaag tcagttttgt ttacgataat caactactgc
    15781 atctcaaaca gggcatttca gcgtctggtg cacgttacac tgacggaatc tatgttttct
    15841 ggtcgaaagg cgatgaagcg actgtctata aacgcgaccg catcgtattg aataactgtc
    15901 agttacaaaa tccacagcgt tgagattttt ccaggggcgg cgcacaatag cgtcacccac
    15961 tgacaatccg taaagaaaac catgtctgat aacgacgaat tgcagcaaat cgcgcatctg
    16021 cgccgtgaat acaccaaagg cgggttacgc cgtcgcgatc ttcccgccga tccattaacc
    16081 ctttttgaac gttggctctc tcaggcgtgt gaagccaaac tggcagaccc taccgcgatg
    16141 gtggtcgcta ccgtggatga acatgctcag ccttatcaac gcatcgtttt actcaaacat
    16201 tacgacgaaa aaggcatggt gttttacacc aacctcggta gccgtaaagc acatcaaatt
    16261 gaaaataatc cgcgcgttag cctgctgttc ccgtggcata cccttgaacg ccaggtgatg
    16321 gtgatcggta aagcagaacg actttcgact ctcgaagtga tgaaatattt tcatagccgc
    16381 ccgcgtgata gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc
    16441 ggtatccttg aaagtaaatt cctggagctg aagcagaagt ttcaacaggg cgaagtgcca
    16501 ttgccgagct tttggggcgg ttttcgcgtc agccttgaac agattgagtt ctggcagggc
    16561 ggtgagcatc gcctgcatga ccgctttttg taccagcgag aaaatgatgc gtggaagatt
    16621 gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt aatcgctggt actcctgatt
    16681 ctggcacttt attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg
    16741 cagtcgttat atacatggag attttgatgg caagcagtaa cttgattaaa caattgcaag
    16801 agcgggggct ggtagcccag gtgacggacg aggaagcgtt agcagagcga ctggcgcaag
    16861 gcccgatcgc gctctattgc ggcttcgatc ctaccgctga cagcttgcat ttggggcatc
    16921 ttgttccatt gttatgcctg aaacgcttcc aacaggcggg ccacaagccg gttgcgctgg
    16981 taggcggcgc gacgggtctg attggcgacc cgagcttcaa agctgccgag cgtaagctga
    17041 acaccgaaga aactgttcag gagtgggtgg acaaaatccg taagcaggtt gccccgttcc
    17101 tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa caactatgac tggttcggca
    17161 atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt aaccagatga
    17221 tcaacaaaga agcggttaag cagcgtctca accgtgaaga tcaggggatt tcgttcactg
    17281 agttttccta caacctgttg cagggttatg acttcgcctg tctgaacaaa cagtacggtg
    17341 tggtgctgca aattggtggt tctgaccagt ggggtaacat cacttctggt atcgacctga
    17401 cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt tccgctgatc actaaagcag
    17461 atggcaccaa atttggtaaa actgaaggcg gcgcagtctg gttggatccg aagaaaacca
    17521 gcccgtacaa attctaccag ttctggatca acactgcgga tgccgacgtt taccgcttcc
    17581 tgaagttctt cacctttatg agcattgaag agatcaacgc cctggaagaa gaagataaaa
    17641 acagcggtaa agcaccgcgc gcccagtatg tactggcgga gcaggtgact cgtctggttc
    17701 acggtgaaga aggtttacag gcggcaaaac gtattaccga atgcctgttc agcggttctt
    17761 tgagtgcgct gagtgaagcg gacttcgaac agctggcgca ggacggcgta ccgatggttg
    17821 agatggaaaa gggcgcagac ctgatgcagg cgctggtcga ttctgaactg caaccttccc
    17881 gtggtcaggc gcgtaaaact atcgcctcca atgccatcac cattaacggt gaaaaacagt
    17941 ccgatcctga atacttcttt aaagaagaag atcgtctgtt tggtcgtttt accttactgc
    18001 gtcgcggcaa aaagaattac tgtctgattt gctggaaata atgcattaag tggaaagggg
    18061 gagtgagaaa tcactccccc tggtttttat acagggaaca tgatgaaaaa tattctcgct
    18121 atccagtctc acgttgttta tggtcatgcg ggtaacagtg cggcagagtt tcctatgcgc
    18181 cgcctgggcg cgaacgtctg gccgctgaac accgttcaat tttctaatca cacccagtac
    18241 ggcaaatgga ctggctgcgt gatgccgccc agccatttaa ccgaaattgt gcaaggcatt
    18301 gccgccattg ataaattaca cacctgtgat gccgtgttaa gtggctatct gggatcggcg
    18361 gagcagggtg aacatatcct cggtatcgtc cgtcaggtga aagccgcgaa tccgcaggcg
    18421 aaatattttt gcgatccggt aatgggtcat ccggaaaaag gctgtatcgt tgcaccgggt
    18481 gtcgcagagt ttcatgtgcg gcacggtttg cctgccagcg atatcattgc gccgaatctg
    18541 gttgagctgg aaatactctg tgagcatgcg gtaaataacg tcgaagaagc ggttctggca
    18601 gcgcgcgaac tcattgcgca agggccacaa attgtgttgg ttaaacacct ggcgcgagct
    18661 ggctacagcc gtgaccgttt tgaaatgctg ctggtcaccg ccgatgaagc ctggcatatc
    18721 agccgtccgc tggtggattt tggtatgcgc cagccggtag gtgttggtga tgtgacgagc
    18781 ggtttactgc tggtgaaact gcttcagggg gcaacgctgc aggaggcgct ggaacatgtg
    18841 accgctgcag tctacgaaat catggtgacc accaaagcaa tgcaggaata tgagctgcaa
    18901 gtggtggctg ctcaggatcg tattgccaaa ccagaacatt acttcagcgc aacaaagctc
    18961 tgaaatatct catttcggcc cacaacgacc tgtgggccga tggctattac tttaagcctt
    19021 ccgctgacag tgcgtcttgt acttccggac gttcagccat acgttgcata aatgctgcaa
    19081 tgtgctctaa cccttccaga ttcagtttca ccgcgtatgc ccagcgcaga accgtaaaca
    19141 gataggcatc agcaattgta aatctttgcc cgcagatcca gtgctcatcc ttcagtgtct
    19201 cgttcacata ttgcagcttc ttctccagct gcgcgcgaac tgtcggtttg tactcttccg
    19261 gtgtatcagg gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg atgtaattca
    19321 gccattcgat ggttttatag cgggaaatac tgtttaccgg tgccagcaac tggcggtcgg
    19381 ggacgctgtc ggcaagatac tgcataatcg ctacgccttc cgtcagcaaa gtaccgtcat
    19441 ccagcagcaa tgcaggcacc tgccccttag ggttaacggc aaagtaatcg tcaccgtttt
    19501 cgagacgttt tttcattaaa tccacactga cgagggtaaa atcctttccg ctctcacgca
    19561 gggtgatatg ggaagcgaga gagcaggcac ccggtttgta gaacaatttc atcggtaact
    19621 ccttttgtct gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa agccgctaac
    19681 gaaaagttag cggcttgtaa aagagtttcc ctaaatttta cgctacagca gctttcgccg
    19741 ctttgtctgc agcgtcatcc tgcgtcatac ggtgcagttt aggcgcggtc agcagcatca
    19801 gtactgcaat aatggcagta gcgacaccaa tctgcaagaa tacgcgacca taaacttcca
    19861 gtgacatcag cgggtcggta acgttatccg gcacagccat catacccgca acataaccac
    19921 caatcaggtt tgcaccggca gtggtcagga accagctacc cataatgaag cccatcagac
    19981 gctgcggaac gagttgtgca accattgcca gacccagacc agagatcatc agttccccga
    20041 tgctctgcag gccatagctt gcgaccagcc agcttacaga cacgatacca gcgtcagacg
    20101 cgaatttcgc acccagcggc agaatcagga acgcaccaga acacatcacc atgccgattg
    20161 caaacttggt tggcatcggc agggtatcgc ccatcttgtt atagatagcg gccagaatcg
    20221 gactaccgat gatgatccag aacgggttca gtgcctgata ctgctcaggt tctacggcca
    20281 gacccagaat ggagtgctca acgttacgaa tcgcaaagaa gttcagtgac gttggcatct
    20341 ggctgtacag cacgaagaag ataatggctt cgagcatcag gatgaaggca acgatcattt
    20401 tacgacgcgc agcacctttc atcgcgaagg cttctttacc gaagataacc acgataccga
    20461 aggcaacaac gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc caggtggcga
    20521 tagcgatcag tgccacaaca ccaataatgg tcagtagcag gttacggtag ttgataggct
    20581 cgaagtctgg ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg aagttaacga
    20641 tagtgatcag caggcctaca acgctcaacg caaacgcaac actccagccg tatttcgcgg
    20701 ccagccacgg cgtagcaatc atagagaaga aagagccgat gttgacggac atgtagtaca
    20761 tggtgaatgc accgtccaga cgcgggtcgt ttttctcata gcatgtagaa agcagagaag
    20821 acgggttagc tttaaacagg ccgttaccga ccgcaatagc cgccataccc atataaacga
    20881 taccggcgtc gtgaccagac caggcaacca gagcataacc aatcgccagc acaatagcgc
    20941 cgagcataat tacgcgttta gtacccagta ccttgtcacc taaccagccg ccgatagcga
    21001 ccagaccata aaccagggca ctaaaggaag agaaaagggt gattgaatcc gcttcagaca
    21061 tacccagttg tttaaccagg taaacagcca taattccttg taggccgtaa taaccaaaac
    21121 gttcccataa ctcaatcgag aagatgagat agaacgcctt cggttgtttg aaagcgttca
    21181 aactgacgct ttcagttggt ttttggtttg cagtggacac ttttacctct ttttttacat
    21241 cccatattaa cgggggtgct ttctcgcgtt atgtctaaaa aacatacgcg caattgttat
    21301 agtgggaggg gaaacggcag gtaatgttca ctatcttgca ctttcagaca agtcctttgt
    21361 aataatttgt tacatataac tgaggcgggg aattcaacca ttccgggaaa tgttatccag
    21421 cgttaaattt ttcaaaaaac gtaacggcag attttttcac tgcactgaga ggggggtgat
    21481 ggcttaacgg cgatatgttc tgctatttag cgtttttact agtgagatag tccagtttct
    21541 gaaaaatagc cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta aatttaaatg
    21601 attttaacct gattcttgca atgtttttac attgcgacag catcgtgatc ttgatcacga
    21661 atgaatagaa aatttcgcta ttaaaaaaac aattaacctg catttttggt tatccgcaaa
    21721 cggattacct gatgataacg ctaagcaatg gcatcattca cgttaaaaag atgttttaaa
    21781 agaggataaa gaaaggttat caatggggta atcggtgtta ccccttttct tcagatgtca
    21841 actttctctt tgtattcaca aagatcttca ataatacaag agccacagcg gggcttgcgg
    21901 gcaatgcagg tataacgccc gtgcaggatc aaccaatggt ggcagtcgac tttaaactct
    21961 gccggaacca ctttcagtag cttttcttct acctgttcaa cgtttttccc cggcgcaaat
    22021 tgagtacgat tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat
    22081 gcagtgttta atacgacgtt ggctgtttta cgacctacgc cgggcagggc ttcaagcgca
    22141 gcacgatctt ccggaacctc gccattatgc tgctccagta agatacggca ggttttgatg
    22201 atattttctg ctttgctgtt ataaagccca atcgttttga tataggtttt aaccccttca
    22261 acgcccagtt caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt cgccgtcgcc
    22321 ttattaacac tgacatcggt cgcctgagcg gaaagcagta cggcaatcag caattcaaaa
    22381 ggcgaactga aattaagctc ggtggtggga tgaggattgt tctcacgcag gcgagtgagg
    22441 atctccaggc gttttgcttt gttcatcaga cattccctgt ttcaccgttt ggcaatgcac
    22501 gttcggcagc tgcttcagta cggcgctttt tcattctttc atcaatcagg tattttcctg
    22561 ccagcatcag tcccaggcca ataaatgcac ctggtggcag catcgccagc aggaaagggg
    22621 agtcggtgtg gaaaatctcc acgcgtaata cttttgccca gctacctaac agcgcatctg
    22681 caccgtcaaa caatgtgcca ttgccgataa tttcgcgtag tgaacccagc acgaacatgg
    22741 cgcaggttgc gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt
    22801 tggcggcgaa ggcttcagcg cggcccacaa cgatacagtt agtgacaatc agcggaataa
    22861 aaatccctaa tgattgatac aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc
    22921 tgaccaccga ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt
    22981 gacgcagcgt cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc gcaagtccta
    23041 aacccagagc attagtggca gtggacgtga ccgccaacag aggacaaagg ccgagcaact
    23101 ggaccagcgc agagttgttt ttccacaacc cctgaacaat aacgtcttta atttcgctca
    23161 cgctttattc tccacaggca ggaagttgag aaagttgtgc cggtaacgtc tgagcgtaca
    23221 atccggcgcg ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg ccggtgaact
    23281 ggtcgaaatc accaccatct ttcttcaccg cccagtgcgc atcatccgca ccactgattt
    23341 ttttacccgc aaaatgggtg atccagtcag aaaggcgcag ttcgatttta tcgccaagcc
    23401 ctggcgtttc gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc
    23461 ccaccagcag ctgaatcgca ccggaatagc catctggcgc ggttgcttcc agaacggcgg
    23521 ctaccggttt gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta cctaactctg
    23581 gcgcagttac cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg gctggcagca
    23641 cctgatcaaa taatgccttt tgttgcagac ttgcctgttc agcaatcgtc gttttggtca
    23701 tctggttgat ggccgcagtt aaccctgttg aacccgctgc aaatagcgct aacgtaatgc
    23761 cgtgttttcg gatagttttc agcatggttt accctttgcg atggccgtag acgcgcggac
    23821 gcgtgtagta atcgatcaga ggaaccgtga tgttcgccag caggacggca aaagccacgc
    23881 cgtcaggata gccgccgaaa ctgcggatca accagactaa taagcccgca agcgcgccga
    23941 aaataagacg accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg
    24001 cgccgagcat ggtcgcacca gacagcagat gaatttgcgg tgctgccagt gtttctggtg
    24061 agaacaacca gcccaacgtt gcgcataacg ccagcgttac taagaagctg aggggaatat
    24121 gccagcgaat cgctttctgc catagcaacc acaggccgcc agccagccag gcgagattta
    24181 cccattgcca accagcgccc gccagaatac cgctgtagat cggatattgc ataatctgtt
    24241 caaccgaatg accggcacgg acagaggttt taaatgtatc cagcggtgtc gcctgactaa
    24301 tgccatcaat acctaagtgt agtgtgttca tatcagcacc actggcggta tgcccgctga
    24361 aaataacctg tatggcgtcg ataaaaccag ggatgttgac cgcaatttca tgtggcggta
    24421 accagctggt catctgcacc gggaaggaga tcagtaagac cacataacca atcattgccg
    24481 gattaaacgg gttttgcccc agaccgccat acaactgttt agcgatgatc accgcaaaca
    24541 ccgtacccag cacgaccatc caccatggcg cgaggggggg aatacttacc gccagcaata
    24601 agcctgtcag caatgctgag ttatctttca acgttgcggc taccgactgc ttgcgtagtt
    24661 tgagtacgag agcttcggct aacagagtac taaccgatgc caacaggatc tgaacgagag
    24721 taccccaacc aaaaaaccac agttgcgctg cgattcctgg cacggctgcg agcaacacca
    24781 acagcataat gcgcgatgtc tggcgctggt tatgggtata aggggagcta gctattctga
    24841 ataccattta gtcctcgttt acaacctttt gctgggcggc ttttttggcc tgagcgcggg
    24901 caatagccgc ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt tctgcgttag
    24961 tctgttgctg ttccagcttg cgcgctttgg cacgggcaat agcggcttcg acggcggctt
    25021 tgcgcggatc aacctgttct tcaggtaccg cattagcctg ttgctgttcc agcttgcgtg
    25081 ctttggcgcg tgcaatagcc gcttcgacgg cagctttgcg cggatcaacc tgttcttctg
    25141 gctccgcatt agcctgttgc tgttccagtt tgcgcgcttt ggctcgggca atagcggctt
    25201 cgatggcggc tttgcgcgga tcaacctgtt cttctggttc cgcattagcc tgttgctgtt
    25261 ccagcttgcg cgctttggca cgggcaatag cggcttcgac ggcggctttg cgcggatcga
    25321 cctgttcttc tggttccgca ttagcctgtt gctgttccag cttgcgcgct ttggcgcggg
    25381 cgatagctgc ttcaacggca gttttacgtg gatcagcaac ggttgctgcg tcgttagttt
    25441 gctgcagttc tgcctgtttc gctctggctt gcgctttacg ggcttcccgt gctgcaataa
    25501 ttgcactgtt atccgggcgt tcgcccgctt taatcacaat aggctgtgta gcctgggcct
    25561 gtttctcttt cacccgcgcc agagcggcag caatcgcatc tttatctttg gctgcaggtt
    25621 gaacggctgc gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc agacgagcct
    25681 ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga cgaatagccg
    25741 caatttcagc tttttcctga cggaaatatt gcaccagggg aatattgctc gggcaaaccc
    25801 acgcgcaagc cccacattca atgcaatcag caatgttatg cgtggtagct ttatcgtgtt
    25861 gctgaccttt gctgaaccag tacaactgtt gcggcaaaag atccgcaggg caggcgtcag
    25921 cacaggcgct acaccggatg cagctttgtt cttcctgtgg ttcgccaagt tcattggcag
    25981 agggagccaa cagacagttg gtaatcttta cgaccgggac atccagccat ggcaaggtaa
    26041 agcccattag cgggccaccc ataatcacca tttgatcggc agaggggcag aatcgggcat
    26101 cattcaataa atgacgcact ggcgtcccca gccgtgccca gacgttgccc gggcgagcga
    26161 ttgcttcgcc agtcagggtt acaacacgct cggtaatcgg ctcgccatca ataacggcac
    26221 gtttcactgc ataagcagtg ccgacgtttt gcattaatac gccgatatcg gatgaacgcc
    26281 cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca ccgccagaag
    26341 gatatttggt tggaatcacc cgcagagaaa tatcgttaga gtccgccagc accgcgcgca
    26401 gcatggaaat cgcctgcggt ttgttatctt caatgccgat aagaatttcg cgtggctgca
    26461 gaatatgcgc aagaatgcga ataccctcta cgacctgagc cgcgcaatcc tgcatcaaac
    26521 ggtcatcggc ggtaatgtac ggctcgcact cagccgcgtt gataatcaac gtttcaatct
    26581 tatctccgcc accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc agcccggcaa
    26641 caccaaactg atgtatgcgc tcgattaact cttcgcgact acgagtgcga taatcggccc
    26701 agccgtcgcg cgggatccag cagtcttcac catcggcatc aataatcacg cttaattcag
    26761 ctaaagctga aggatgagcc gtagagtggg gcgcaatagc cgtaacggta cccgaggtgg
    26821 gcgcgtgaac aggcagcatt ttgccgcgac cacgggtaag cggctggccg cgcaatactt
    26881 tatcgccgac gctaacgcac aactcacctt cagcgccaat atgctgtttc agtggaataa
    26941 caaaacgctg cgccagtggt acctggcgca ggggtgtacc gttggactgg gttttcatct
    27001 ccggtggatg gatgccgccg ttgaaatccc agattttatt ttttctgaat gcagagaata
    27061 acttaagcat ggagttccac gggaatgata cgcacgggaa tggtgttcag atcccatttc
    27121 caggagtcag gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg gcacggatca
    27181 acacataaat tgcagcccgt acagagatca ctcattaccg tatgcatggc acgggtagcg
    27241 ccaacgatgg cgtctaccgg acaagcctga atacatttag tgcagccaat acagttattt
    27301 tcatcaataa ccgccaccat ccgcgcaggc gttagctctt gcgcttcgcc atccagcggc
    27361 tgcggctcga cattaagcaa ctcggcaatt tttagcatca cagcttcgcc acctggggcg
    27421 caacggttga ttttttcacc gttacagctg atggcttccg cgtaggggcg acagccggga
    27481 taaccgcact gaccacactg gctctgcggt aagatttcgt caattttctc aacgaccgga
    27541 tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa cgccaggccc
    27601 agcaggctca cggcggcaac ggcaatccag atagcattca ttacaacttc accaaaccac
    27661 taaagcccat aaaggccaga gacataagac ctgcggtaat taacgcaatg gcattaccgc
    27721 gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg gatggcggtg aagagcacca
    27781 tcaccagcga gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc aagaaattgt
    27841 gcccgagatt gatattcagc aacgccacgc cgagcactgc acagttggtg gtgataagcg
    27901 gcaaaaaaat ccccagcaga cggtaaagca ccgggctggt tttgcgcacc accatctcgg
    27961 tgaactgcac gaccacagca atcaccagaa taaatgccag ggtgcgcagg taaatcagat
    28021 taagtgggat caaaatccac gtatcgataa gccaggcgca aatagacgcc agcgtcatca
    28081 caaacgttgt tgccagcccc atgcccatcg cggtttccag ttttttggaa acccccataa
    28141 acggacagag accgagaaac ttgaccagca caaagttatt gaccagtaca gttccgacaa
    28201 acagtaacag gtagtcagtc atttttcggc ctgaaataaa aaagccgcct attatccgtt
    28261 aatccagagc aggcgacaac aggttaactg taaggttatt acggattcac aaacgtcgct
    28321 tttacccgcg acgagcgttt gaaataggag acgatcaatg cggttgccaa caaagtaaac
    28381 agcaactggc gaacggctat gccatcttcc acgggtgaaa aagcgaatgc ttttactgcc
    28441 agcaatacgg aaataagcag ccagataata tagtgtttag gaacacaacg ccgacgttta
    28501 aagaaagcga tggtcaacca cagtgtgtaa taccacatgg cgatagcggt aataaaagaa
    28561 acgccccata agatttgtgt cgtcagtgct tgtccgccga gcgtttgaaa tgtttgcgga
    28621 gaagataacg cagcggtata cagcaacaac gccagcgtcg tactcaacag agcgactaaa
    28681 agccaggcca acgggccaag caaccagcct ccaatacgtt gtggcgttgt ggtggtcata
    28741 ggttctccag aatagcgacg caaacaggca aatctaattt taggggcgtt attataaggc
    28801 ttttataacc gatcgctact ttctttattg gacatagtgc cagacggact tcggtacctg
    28861 gccgaggtca aacaaacgcc cgccagaaac cagctctgcg cgacggtgat cggcagcacg
    28921 atacatattg atcagttcct gatcgtccgt cagggtgtaa ttaagatggt cgtagagttt
    28981 ttccagactt tcgagtgaac tgattttgcg aaattttaat aagtagtcct gaacagtcat
    29041 aaaaggtcca taaaataatt aataccaaag gggtaggtat tattagtgat gataaaattt
    29101 caccaaaagt gaacgcttgt gttattacaa taataagaat gcgctgatgg caaatattca
    29161 gggatgaaaa actgctctga aaagagtaac gcgctttact atccctgaca atcaccaaca
    29221 acatcgaaca agataataaa ttcctggttt aatatccgac aagtgaaaac atgcaaccgg
    29281 acgggcagca tggcgctcta caagcgcaga gcttacttgt gttgtaccga agcactctgt
    29341 tcaggtggct gatagttgtc aatgtgactc gccacgccaa gaagaatgac tgaaacgaca
    29401 agaacgatcc aacctgttaa ttcaataaga cgattcatta cagcccacat ctctcttgat
    29461 tgatccatta acttcagggg gtaaatgtta cttagcaata atagctccag caagattttt
    29521 actgaggttt ttgcgatatt tagcttttgt cgttggaaaa ttcgctattt ttttgactta
    29581 atattaaact gttgagcgtg ggtaaaggat agtgtcaaat agcaatcata cttcaatgag
    29641 aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt atgcgagcaa
    29701 aaccttccat gcgcccctga ttgcgggcac gcccgggctg gaactggcgg taatctccag
    29761 cagtgatgaa acaaaagtaa aagccgactg gccaacggtt acggttgtct ctgagccgaa
    29821 gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca acgataccca
    29881 tttcccgtta gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg ataaaccctt
    29941 taccgtgaca ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc tggggcgtgt
    30001 gctgtctgta ttccataacc gtcgttggga tagcgatttc ttgacgctaa aaggtttgct
    30061 cgcggaaggc gtgctgggtg aagttgctta ctttgagtct cattttgacc gcttccgtcc
    30121 gcaggtgcgc gatcgttggc gtgaacaggg cggcccaggc agcggtatct ggtacgattt
    30181 agcaccgcat cttcttgatc aggccattac gctatttggt ttaccggtca gcatgacggt
    30241 agatttggca cagttacggc ccggagcgca gtcgaccgat tatttccacg ccatcttgtc
    30301 ctatccacag cggcgagtca ttttacacgg taccatgctg gcagccgctg agtcagcacg
    30361 gtatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc cacaggaaga
    30421 acgtctgaaa aatggcgagc gtctgccgca ggaagactgg ggctacgata tgcgtgatgg
    30481 cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga cggtacctgg
    30541 gaattatccg gcttactatg cggctattcg tgatgcgtta aatggcgatg gtgaaaatcc
    30601 ggttccggca agccaggcaa tccaggtaat ggagttgatt gagctgggca tcgaatccgc
    30661 caaacatcgc gcgactttgt gccttgcatg aaaaatggat ggtgcaattt gcaccatcca
    30721 ttttgttact tcgcggcgac tttttctcgc agtgcgcgtt tttcctcagc gctgaggaaa
    30781 gccatttcca gaccattaat ctgtgcctgg cggatttgct cgcgggataa cccagcagct
    30841 ggcgcggcaa cggtatattc gtgaatgata tccactccct gtacgccggg atcgtcagtg
    30901 ttaatgccgg cacgaatgcc atgctcaagg aacgttttca gcggatgtgc agccagctct
    30961 gctacggtgc tggtctgaat attggaggtc agacaggatt caataccaat ttgttgctcg
    31021 gcgagaaaat ccatcagcgc ccgatcttca atggctttta cgccatgtcc aatacgctcc
    31081 gcacccagtt cacgaatcgc ctgccagatg ctttccggcc cggcagcttc gcctgcatgg
    31141 acggtaatat gccagcccgc atcacgcgca aggttgaagt gagaaaggaa cagacttccc
    31201 gggaaaccaa gttcatcacc ggctaaatca agggcggtaa tctggtcacg gtgggctaaa
    31261 aaggcctcca gctcttgctg acaggcggct tcgccgaagg ttcggctcat aatgccgata
    31321 agcttcgcct gcacaccaaa agtgcggcaa ccttcacgta cgccatcgat caccgcttcg
    31381 acaacacccg ctacaggcag cttatgtgcc attgccatgt agcctggtga aaaacgcagc
    31441 tcgacatagt gcaggccgtt acgggctgca tcttcaatgt tttcaaatgc cacgcggcgg
    31501 caggcatcaa gagaggcgag aactttaacg ccccagtcaa gtttggtcag aaagctcacc
    31561 agatcgggtt cgttggcaat gacctgaacg tggggaatca gtgtttccag ggattgtgca
    31621 ggaagcgaga tattatactg gcggccaagt tcaagaatgg tctggggacg aatgttgcca
    31681 tcaaggtggc gatggatatc agttaatggc agggtggtat caatcatggt cgcactcttt
    31741 ttctggatat aaaagtgcgc accattataa aaacaaagca gggtaaaaat ccacgattgc
    31801 gcaacgattt ttacgttgcg caattggggt taacgaacag cgcggatggc gttaattaat
    31861 ccagccacac ctttttccag tttcgaacgt gggcagccgg cattgagacg gacaaaacca
    31921 cgaccttctt caccgtaggt ataccccggc atgatcgcga ctttttcttg ttcgataagt
    31981 gctttttgca acgcgttgtc gtcaatattc aacggacgta aatcaagcca tgccagataa
    32041 gtggattgtg ggatctgcca gttgagttca ggaaacgcgg cgttcatttt atctgcgata
    32101 tacgtcaggt tatctttcag atagatgcgt aaggcatcca gccacggcgc gccttgctga
    32161 taggcggcga tatgggcagt taacgccagt accgaagggg aagaaagccc atcacggcct
    32221 ttcagtgccg ataaataggc atcgcggcta ctgctatttt ctataatccc gtaagcaccg
    32281 gtcagggcgg gaatattgaa acttttcgag cccgacgtta gcaacgccca gtctccgcga
    32341 gccacattac tccagggaat atgcggctgc tcgccccaaa ccatatccat atggatttca
    32401 tcggaaataa cccgcacacc atgacgctcg cacaggtcag ccatgatctc cagctcatcg
    32461 cacgtccaca ctttcccggt aggattctgt gggctacaca ggagcataat tttacattct
    32521 ggtttcgcca acacggcttc caacttgccc atatcgcaaa accaaccatc agcctgcttc
    32581 tctaaagcaa cgggcattac tgtgcgctgg ttaccttcaa tggccttgta aaatgcgtca
    32641 taggcgggtg tgtggatcac cacgccttca cctgtttcag accactgacg aatcagttct
    32701 gaaaccatat agatgacaga agggccatac accaccgtct gagaatcgat ggcggtgtaa
    32761 tgctgggtgg aaaaccagtg ggcaatagca gcgagaaact catcgttttt ccagcggctg
    32821 tagccaaata cgccgtgcat caggcgctga ttcagcgcct ctataatgca gggggcagtg
    32881 gcaaaatcca tgtctgaaat cgtgaacggt aacaggtcag cagtgccgaa acggtcagcg
    32941 acataatccc actgtgtaca ccatgtgcca tgacgatcca cgacctttga aaaatcgaac
    33001 atatcttatc cttatgcctg gacagtatgc atcagaccgg ccatttcatc ttttactgac
    33061 tgaacttgtg gcccgataac aacctgcagg ttatgttgat taagttgtac tacgccaatt
    33121 gcccgattgt ccttcagtgc ctgcacatta acaagcgaca tatctttcac agacaaacgc
    33181 agacgggtaa tgcagttatc gaggctgaca atattgtcgg caccgcctaa tgcttcgagg
    33241 attgcaggaa cgttgtaacc tgatttaccc ggcgcagcgg caacggcttt ttcgattgag
    33301 ctggcaactt cgctatcgcg ccccggggtt ttgagattga agcgggtgat agcgaaacgg
    33361 aagatggcgt agtaaacgac aaaccagatt gccgccacca ctggcaccat gtaccacttg
    33421 gttgacagac catgcaaaat accgaacacc acgaagtcga tgatattgcc gtcggtatta
    33481 ccgatggtga cgccgagcac agacatgacg gtgaagccga ggccggttaa cagcgcgtgg
    33541 atgacataca gaactggcgc tacgaacagg aacaggaatt ccagcggttc ggtagtgccg
    33601 ccaacgacgc aggcgatcag gccagaaatc agcagacctt taattttatg gcgattttcc
    33661 gggcgcgcgc agtgatacat agctaacgct gcagctggca gaccgccgag aaacgcaggc
    33721 attttacctt gcgaaaggaa acgcgtggcg ctttcagaaa aaccgtgagt ggtcgggcaa
    33781 ctcaattgcg tctggaagat ggtcagtgcg ccgctgacgg tttgaccgca gacttcctgc
    33841 gtgccgcctg cgtcggtaaa gcgaattaat gccaccagaa tgtgatgcag accaaacggc
    33901 aacagcagac gttcaccggt accaaacagc atcggtccga aatcacccgc gctgtttatc
    33961 atatggccca agccgctaat acccatggcg aaaatcggcc agactaatgg aatcaccagg
    34021 ccgacaaggc ccatcaccag cgaggagata attggtacga agcgcgtacc gccgaagaat
    34081 gccagcgcat ccggcaggcg gatattatgg aaacgctcat gcagcatcca gacgataata
    34141 ccggcgatca ccgcaccgag gatcccggta tcgatcgact ggatcccaag aatgctctgg
    34201 atgttattgg ctttcagaac cgcggcatcc gtggttggca gaatgccttt attggtcaac
    34261 cagaagttta ccgcgaggtt cattaccgca taaccgacga agccagcgaa tgccgctacg
    34321 cctttatttt cgcgtgccag gcccagcggg atggcgatac agaacatgac aggcaggaaa
    34381 ctaaaagcaa acgagccaat cttactcatc caggtaaaga tagcctgcaa cacggggttg
    34441 cccaggaccg ggatcagggt tatgacatca tggctgctaa gagaactacc aatgccgagc
    34501 ataatgccgc agaacgacaa taatgccacg ggtaacatga aggttttgcc taactgctgg
    34561 aagaactccc acagcgtgac tttcggtgct gttttcgccg tcataaaacg actcctcgta
    34621 gagaacaatc tggattcagt aaattgcgcg agaagataaa acgttttatc aaattttagt
    34681 gaggcataaa tcacattacg caacgataat agcgggtata agataaataa aaggtaaaac
    34741 gttttatctg tcacataatc agggagtagg tcatctgcat ggctaccgcc aaaaaaataa
    34801 ccattcatga tgttgcgctg gctgcgggcg tgtcggtaag taccgtttcg ctggtgctta
    34861 gtggcaaagg gcgaatctct accgccacag gagaacgcgt taacgccgcc attgaagagc
    34921 tgggatttgt gcgcaatcgc caggcgtcgg cgctgcgcgg cgggcaaagc ggcgtcattg
    34981 gtttgatcgt ccgtgattta tctgcgccgt tttacgccga attgacggcc ggattgacgg
    35041 aagctctgga agcgcaggga cggatggttt ttttgcttca cggcggtaaa gacggcgagc
    35101 agctggcaca gcggttttca ctgttactga atcagggggt cgatggtgtg gtaattgccg
    35161 gggctgcagg aagcagcgat gacctgcgac ggatggcaga agaaaaagct atcccggtga
    35221 ttttcgcttc ccgtgccagt tatcttgatg atgttgatac ggttcgcccg gacaacatgc
    35281 aggctgcaca gttgttgacg gagcatctca ttcgcaatgg gcatcagcgg atcgcctggc
    35341 tgggagggca aagttcctca ttaacccggg cagaacgggt gggaggctat tgtgcaactc
    35401 tactaaaatt tggtctgccg tttcacagcg attgggtgct ggagtgcaca tccagccaga
    35461 agcaagctgc ggaagctatc acggcgcttt tacgtcataa cccgaccatc agtgctgtgg
    35521 tttgctataa cgaaactatt gcgatggggg catggtttgg tttgctgaaa gcagggcggc
    35581 aaagcgggga aagcggagtc gatcgttact ttgagcaaca agtttcgctg gcggcattta
    35641 ccgatgcgac accaaccaca cttgatgata tacccgttac ctgggccagc acgcctgcgc
    35701 gggaacttgg taccacactt gcggatcgca tgatgcaaaa aatcacccat gaagagacgc
    35761 attcacgcaa tcttattatt cccgcccggc tcattgcagc gaaataatcc tctctttatc
    35821 tgctatacct ggtagtgtcc tttcctcaag gttaatgata aaaaatgtgg cacacaggcg
    35881 g
//
LOCUS       DAJVOY010000020        35055 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVOY010000020 DAJVOY010000000
VERSION     DAJVOY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.020"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..334)
                     /locus_tag="OQL90_000887"
     CDS             complement(<1..334)
                     /locus_tag="OQL90_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1367751.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="OQL90_000888"
     CDS             complement(415..1842)
                     /locus_tag="OQL90_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1367752.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="OQL90_000889"
     CDS             complement(1839..2546)
                     /locus_tag="OQL90_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1367753.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2709..3686
                     /locus_tag="OQL90_000890"
     CDS             2709..3686
                     /locus_tag="OQL90_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1367754.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="OQL90_000891"
     CDS             complement(3756..4238)
                     /locus_tag="OQL90_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1367755.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="OQL90_000892"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="OQL90_000892"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1367756.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="OQL90_000893"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="OQL90_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1367757.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="OQL90_000894"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="OQL90_000894"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1367758.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="OQL90_000895"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="OQL90_000895"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1367759.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9957
                     /locus_tag="OQL90_000896"
     CDS             9349..9957
                     /locus_tag="OQL90_000896"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCS1367760.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPPLTARGIEQAQNLHTLLHGV
                     SFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAENY
                     SAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARLI
                     GMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVDNA"
     gene            10217..10534
                     /gene="rsfS"
                     /locus_tag="OQL90_000897"
                     /gene_synonym="rsfA"
     CDS             10217..10534
                     /gene="rsfS"
                     /locus_tag="OQL90_000897"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1367761.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10538..11005
                     /gene="rlmH"
                     /locus_tag="OQL90_000898"
     CDS             10538..11005
                     /gene="rlmH"
                     /locus_tag="OQL90_000898"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1367762.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11036..12937
                     /gene="mrdA"
                     /locus_tag="OQL90_000899"
     CDS             11036..12937
                     /gene="mrdA"
                     /locus_tag="OQL90_000899"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1367763.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12940..14052
                     /gene="mrdB"
                     /locus_tag="OQL90_000900"
                     /gene_synonym="rodA"
     CDS             12940..14052
                     /gene="mrdB"
                     /locus_tag="OQL90_000900"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1367764.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14063..15151
                     /gene="rlpA"
                     /locus_tag="OQL90_000901"
     CDS             14063..15151
                     /gene="rlpA"
                     /locus_tag="OQL90_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1367765.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15291..16502
                     /gene="dacA"
                     /locus_tag="OQL90_000902"
     CDS             15291..16502
                     /gene="dacA"
                     /locus_tag="OQL90_000902"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1367766.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16613..16876
                     /locus_tag="OQL90_000903"
     CDS             16613..16876
                     /locus_tag="OQL90_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1367767.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16977..17618
                     /gene="lipB"
                     /locus_tag="OQL90_000904"
     CDS             16977..17618
                     /gene="lipB"
                     /locus_tag="OQL90_000904"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1367768.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17877..18830
                     /locus_tag="OQL90_000905"
     CDS             17877..18830
                     /locus_tag="OQL90_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1367769.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19039..20004
                     /gene="lipA"
                     /locus_tag="OQL90_000906"
     CDS             19039..20004
                     /gene="lipA"
                     /locus_tag="OQL90_000906"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1367770.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OQL90_000907"
     CDS             complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OQL90_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1367771.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20437..21225)
                     /locus_tag="OQL90_000908"
     CDS             complement(20437..21225)
                     /locus_tag="OQL90_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS1367772.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENAVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21318..21701
                     /gene="crcB"
                     /locus_tag="OQL90_000909"
     CDS             21318..21701
                     /gene="crcB"
                     /locus_tag="OQL90_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1367773.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OQL90_000910"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OQL90_000910"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1367774.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OQL90_000911"
     CDS             complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OQL90_000911"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1367775.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23289..24674
                     /gene="dcuC"
                     /locus_tag="OQL90_000912"
     CDS             23289..24674
                     /gene="dcuC"
                     /locus_tag="OQL90_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1367776.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OQL90_000913"
                     /gene_synonym="citB"
     CDS             complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OQL90_000913"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCS1367777.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OQL90_000914"
                     /pseudo
     CDS             complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OQL90_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            27370..28428
                     /gene="citC"
                     /locus_tag="OQL90_000915"
     CDS             27370..28428
                     /gene="citC"
                     /locus_tag="OQL90_000915"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1367778.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28443..28739
                     /gene="citD"
                     /locus_tag="OQL90_000916"
     CDS             28443..28739
                     /gene="citD"
                     /locus_tag="OQL90_000916"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1367779.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28736..29644
                     /gene="citE"
                     /locus_tag="OQL90_000917"
     CDS             28736..29644
                     /gene="citE"
                     /locus_tag="OQL90_000917"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1367780.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29655..31187
                     /gene="citF"
                     /locus_tag="OQL90_000918"
     CDS             29655..31187
                     /gene="citF"
                     /locus_tag="OQL90_000918"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1367781.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPTR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31191..31741
                     /gene="citX"
                     /locus_tag="OQL90_000919"
                     /pseudo
     CDS             31191..31741
                     /gene="citX"
                     /locus_tag="OQL90_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31716..32593
                     /gene="citG"
                     /locus_tag="OQL90_000920"
                     /pseudo
     CDS             31716..32593
                     /gene="citG"
                     /locus_tag="OQL90_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            32644..34107
                     /gene="citT"
                     /locus_tag="OQL90_000921"
     CDS             32644..34107
                     /gene="citT"
                     /locus_tag="OQL90_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCS1367782.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34218..35024
                     /gene="rna"
                     /locus_tag="OQL90_000922"
     CDS             34218..35024
                     /gene="rna"
                     /locus_tag="OQL90_000922"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1367783.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPSNCGKTFVIDKVGY"
BASE COUNT         8728 a         8938 c         8666 g         8723 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc agtggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcgcagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggttc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccaaagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aacacataaa atataatatg ttttatctac aaccataagg
     2701 aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcagctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgctttc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaggaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 cccctgaccg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct acatggtgtt
     9481 tcctttgatc tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt
     9541 ctcagtgacc gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc
     9601 gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg
     9661 tggtgcaatg actggcagca tgcaatcccc acgaacggtg aaggatttca ggcattttcg
     9721 caacgtgtgg aacgctttat cgcaaggctt agtgaatttc agcactatca gaatatttta
     9781 gtcgtcagcc atcagggtgt actgagtctg ttaatcgccc gtttaattgg catgcctgcc
     9841 gaggctatgt ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa
     9901 aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgataa tgcatgactt
     9961 ttctgttttt ttacgggtaa gccgcaacga ccattgacag acccgggcag gctgatattc
    10021 tccgcagcca gacttttttc gccagacacg actttgtaga aattgtttta caaaaatggc
    10081 gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca
    10141 attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt
    10201 cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga
    10261 tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga
    10321 ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt
    10381 tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc
    10441 cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg
    10501 tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt
    10561 gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc
    10621 gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga
    10681 catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg
    10741 cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct
    10801 ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt
    10861 gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc
    10921 ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac
    10981 caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa
    11041 actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct
    11101 ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct
    11161 gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt
    11221 gcctatcgcg cccagccgcg gcattattta cgaccgtaat ggtatccctc tggccctcaa
    11281 ccgcactatc taccagatag aaatgatgcc agagaaagtc gataacgtgc agcaaacgct
    11341 ggacgctttg cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga
    11401 gcgcgcacgt tcacaccgtt tcacctctat tcccgtgaaa actaacctga ccgaagtaca
    11461 agtagctcgc tttgccgtca atcagtaccg ttttccgggg gttgaagtta aaggctataa
    11521 acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat
    11581 caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac
    11641 gcatgatatc ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac
    11701 cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt
    11761 accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat
    11821 tgaaacgctg ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg
    11881 ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ttgtttgttg acggtatctc
    11941 cagcaaagat tattccgcct tgttgaacga cccgaataca ccgctggtga accgcgccac
    12001 acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattgag
    12061 cgccggggtg atcacgcgca atacgacgct gtttgaccca ggctggtggc aactgccagg
    12121 ttcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag
    12181 atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga
    12241 tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc
    12301 ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc
    12361 gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc
    12421 aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca
    12481 tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc
    12541 gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt
    12601 tgctaaccgc cctaacggta cgacgcataa atactttgcc agcgcaccgt acaaaattgc
    12661 ggcgaaatct ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca
    12721 caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa
    12781 tccgcaagtg gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac
    12841 gctgatgcgt cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc
    12901 ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa
    12961 aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg
    13021 ctggtttaca gcgccctagt tatctggagc gccagcggtc aggatattgg catgatggag
    13081 cgtaaaatcg gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct
    13141 ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg
    13201 gcggtagatg ctttcggtgc catctctaaa ggtgctcaac gctggctgga cctcggtatt
    13261 gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt
    13321 atcaaccgcg acgtttgccc accgtcgttg aagaacacag gcatcgcgct ggtgctgata
    13381 tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg
    13441 ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca
    13501 gtagtgctgg tggcggcgtt cattcctatt ctgtggttct tcctgatgca tgattaccag
    13561 cgccagcgcg taatgatgct cctagacccg gaatcagacc cactcggcgc gggctatcac
    13621 attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcat
    13681 ggcactcagt cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta
    13741 ctggcggaag agctgggatt agtgggcatt ttgattctgc tcgctctcta cattctgctg
    13801 atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct
    13861 ggcggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt
    13921 attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt
    13981 gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg
    14041 aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg
    14101 aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc
    14161 tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact
    14221 gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga
    14281 tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa
    14341 cctgacggcc tctggcgaag ctttcgatcc gacacagctg acggcggccc atccaacgct
    14401 tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg
    14461 cattaatgat cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc
    14521 tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca
    14581 ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc
    14641 cctgcctgca cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca
    14701 gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc
    14761 ggtaaccagt agcggtttcc tcggcgcacc aacgacctta gcgcctggtg tactggaagg
    14821 cagcgaaccg acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac
    14881 ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt
    14941 gcaagtcggg gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca
    15001 gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg
    15061 ccccttcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca
    15121 attacagtca tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt
    15181 ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc
    15241 tatagtaggg cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    15301 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    15361 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    15421 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    15481 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    15541 gccaagcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    15601 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    15661 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    15721 gtgtcgccat ggctgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    15781 gctacgttaa cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg
    15841 ctgatggtca gtacagctcc gcgcgcgata tggcgctgat cggccaggcg ttgatccgtg
    15901 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    15961 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    16021 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    16081 tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    16141 tgctgacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    16201 tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag
    16261 acatgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    16321 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    16381 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    16441 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    16501 aaaaattaaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    16561 ctcccgcctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    16621 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    16681 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    16741 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    16801 cgccactcat atcgagcagg ttgaaatact gtatgaagaa ctgggcaaaa tcgatattgt
    16861 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct
    16921 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    16981 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg
    17041 ctatgcatga attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg
    17101 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    17161 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac
    17221 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    17281 tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    17341 ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    17401 gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    17461 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    17521 ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc
    17581 tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    17641 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    17701 attttcttta tcacttgcag aaatgaggtg ccacgccttg ctccgtaact caccccattg
    17761 attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata
    17821 agattattgt aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg
    17881 atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa
    17941 tatttacaac cttacgaaat attgctctta accttctgac tatttttgaa gctgtatatg
    18001 tacataaagg gatcgttaat gcagcgaaag tgcttaatct gcccccctcg gcaatcagtc
    18061 agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccaag
    18121 gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt
    18181 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg
    18241 cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    18301 actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc
    18361 agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc
    18421 atgttctgtt caccgacaat atggtgttaa tttgccgcga gggaaatcca ctactctctt
    18481 tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagagc
    18541 aaaatttcag cggtctgcgg cagagagttc aagaaatgtt tccggaccgg caaatcaatt
    18601 tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga
    18661 ttattcccag ccgtttttat aacctgttta gccgctgctg gccgctggaa aaactgcctt
    18721 ttccgtcctt aaataaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc
    18781 gtgatcccat cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg
    18841 ttacagccag atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt
    18901 ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt
    18961 caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc
    19021 aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc
    19081 gatgccgata agatggccct tatcccggtt aaaaacgtgg caatagagcg cgaagccctg
    19141 ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc
    19201 atcaaagccg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct
    19261 aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt
    19321 acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat
    19381 gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc
    19441 tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact
    19501 gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgtggt
    19561 cgtatggatc gtgctctgga tattctgact gcaacgccac cagatgtgtt caaccataac
    19621 ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg
    19681 ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtcta
    19741 atggtgggac tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt
    19801 catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg
    19861 gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg
    19921 ggctttaccc atgctgcatg cggtccgttt gtccgttctt cttaccacgc cgatttgcag
    19981 gcgaaaggga tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa
    20041 cccgcttttt gaagcggttt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc
    20101 cacgtcactc tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg
    20161 ccgcagcatc atcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc
    20221 cgcccagcgt acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacta
    20281 gcagtttggt aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga
    20341 acgcgcctta aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc
    20401 tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa
    20461 gcgacggttg tttaagacgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac
    20521 ttccgccata atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc
    20581 tataatccgg ctttgcccga tatttttgtt cccgcactcg cccgccgcca ccatataaca
    20641 ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc
    20701 gcgaacccag gcggcaggaa gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc
    20761 cagctctgga aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc
    20821 cagtaacgga gcgatttcat taccagcatc aacacggcgt gattcctgaa tggcaaatgc
    20881 atcatagaga tgcagtttgg cataacgggc gacgatgttt cctgcctgaa gtgccaccag
    20941 catattccat gcgcgccccg gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat
    21001 gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga
    21061 tttaaccgat agatctgcat catgatcatc gcgtgccagc aatgcttccg gcaggacaaa
    21121 cagcgatacg gcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt
    21181 ttcccacaca gatgtaacag caaactgtcc tgcagcaacc agcattgcca cctcctgtca
    21241 gagtcgtaaa attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca
    21301 aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg
    21361 gaagcgtggc gagatggctg ttaagtatgc gatttaaccc actgcatcag gcgattccgt
    21421 tggggacgct gacagcaaac ctgattgggg cattcatcat aggaatggga ttcgcatggt
    21481 tcagcaggat gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg
    21541 gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggtc
    21601 gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgacag
    21661 cactggcttt ctggctgttt tcggcctcaa ccgcacacta aagacccaaa aaaaacccgc
    21721 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21781 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21841 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    21901 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    21961 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgcgtcggt taccagtaca
    22021 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22081 ttttgatgtt acttatttta gctattgatt ttaaagaagt tactaaaact tcatttgtct
    22141 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22201 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22261 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22321 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22381 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22441 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22501 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22561 tgttcaggct gttgccaggt tggtgcaata ttttctctaa acgttgtcat ccactcatct
    22621 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22681 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22741 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22801 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22861 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    22921 atgcaatcaa ggaaaaatta ttttattttt taacgagata caacaatcac cttaacaaag
    22981 tatataatat atatcgtcaa cctatgctca tattagatta ttttttgagc gcacagccgc
    23041 agctaacacc attgcaatta acaaatttgc atcaatccac catcaatttg cacacattat
    23101 tatgtgataa ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagtaac
    23161 ttcaatgtct taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg
    23221 gcatattttt tccttaagct ttaggaattt tttattattt actttggggc ctggagacag
    23281 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23341 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23401 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23461 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23521 acctcggcat gatgattatg atgctgtgtg gatttgccgc ttacatgacc catatcggcg
    23581 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23641 tgctgatgat tgccgcctat tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa
    23701 ccggtctggg tgttttgctg atggcaaccc tatttccggt gatggtaaac gttggtatca
    23761 gtcgtggcgc agctgctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23821 caggggatgt ggtgctggcg gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca
    23881 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    23941 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24001 tcaccaccac tgctcctgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24061 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24121 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24181 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24241 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24301 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24361 caatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24421 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24481 tgcaggcgtc aaaccttggc cgtacccttt cgcccgtttc tggcgtagtc gttgcggttg
    24541 ccgggatggc gaagatctcg ccgtttgaag tcgtaaaacg cacctcggta ccggtgcttg
    24601 ttggtttggt gattgttatc gttgctacag agctgatggt gccaggaaca gcagcagcgg
    24661 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24721 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24781 atggcggctg gcgcaatatt caagataacg tctggcagac gtgcggctga tggtcagtgc
    24841 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    24901 gtttagcgtc aagggatcaa tgccggtcgg cagttcgtct ttaggttcac cgcgagcata
    24961 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25021 tttacgctgg cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat
    25081 gagataatca aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc
    25141 agtggtaaac accacgtcgc cgggataatg cgcctgcacc agttcatgca ataaattaat
    25201 ccctctgccg tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat
    25261 catcattcgg gcctgcgcca ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg
    25321 acgaatatat tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt
    25381 taatggagct gtcattaggt ttcactttcg gaatatagat tgaaaataag gtaccgcagg
    25441 gatcattatc ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt
    25501 acaacccaat gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata
    25561 ttttgtctcg tagagactct ggaacgccgc agccctgatc ggcgacttca atcaccacat
    25621 catcgccttc atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc
    25681 tggcttcgaa ggcgttatca agtaaattgc ccgcaatggc tgcaaactcg gtgctatcca
    25741 gtcctggcgg cagttgcgaa agctggctac cggggacaat gatcattttt agccccagtt
    25801 cccgggcgcg ctgcacttta ccaaaaagca gccccgccac ctagcgatcg gcaaacgcct
    25861 cgcgcaggct gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca
    25921 cgcgatcata ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgagatgct
    25981 cgtgtcgcaa tgtacgaagg ctctcaacgt attgttttat ttgcgtcagt tgcgcattga
    26041 gggtggaaat ttcgtcttta ctgcgaaagc tgataatggc ccccagcaaa tcatcacctg
    26101 aacgaatagc ttcccgattg gcaataacgc tcagaccgtt aaagttagcc accatatcct
    26161 gacgtttttc atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcagcaatgg
    26221 gtttacccaa ccattgccgt ccgggggaac tcagccccag catctttctt gcgttacgat
    26281 tgatggcggt aatgtaaccg tgcggatcca ccgcaatcag cccttcataa accgaactaa
    26341 acagcgcctc ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcatct
    26401 gccgacggat atgcgcggcc aggaaccacg acagcaacat cagaatccct aacagcacga
    26461 caaacacacc tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca
    26521 ggtagccaat cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg
    26581 cgcgcatcgc catccccatt gaccctttcc cggtaatgaa gtagctctcc cctttctcca
    26641 gcgcgccctg tttggtgaac tgcataggat aaccaatttt ctccggatta ggatggtaaa
    26701 ggcggatcga gtgccggtcg ccatcaccac ataatcaaaa tcggtatctc tttgtaattt
    26761 gttagcgatg gtcgccagcc gtttgtagtc acgcgttttc accgcagaga tgacactgtc
    26821 attggaggca ataattttcg cctgattcat tgccatgtcg cgtacatgaa gcgttaaata
    26881 gtcctcaaaa ctggccgtaa aatattgcgc caaggctgca atgacaaata ttgagaacac
    26941 cagaatcagc aaaaagatac gcagcggaaa tgccagtctt tggaaaaatg caaactgttt
    27001 attctcgtta agctgcaaca ttgttttcct tgcgggttat cccgagcctc tttgacagat
    27061 gtaattaaaa tgttgaatca aaatacatag atattaatta tgatcgtggc aaattttatt
    27121 cattaaatca attaaatcac ttaaacctgc aggtcatttc gtaattacat taattaaaaa
    27181 acttaaacca ttaaataaat aaaaatttca cgccgaaata ctccttagga tgtatagcga
    27241 aaggagaaaa agatatacct cgatcacccc cctttctccc aagtgaaaat aaaaggttat
    27301 cagtttgcaa cattgaacaa cattcgttgc aaatcgataa caacatgcac cttcaggata
    27361 ctatttatta tgttcggcaa tgatattttc acccgcgtaa aacgttcaga aaataaaaaa
    27421 atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac cacagtcgaa
    27481 gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat tgccggaaat
    27541 attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc gctgacatta
    27601 gccactgaac tgataaacct cgcctatgag cggcacagca cgcatctgtt tatttatacc
    27661 aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgctgac cagcgtaccc
    27721 ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga aacgctatgc cgaatcgctg
    27781 aaaaaatttc gtcatccagg gaacaagatt ggctgcattg tgatgaacgc caatcccttt
    27841 acgaatggtc accgttatct gattcaacaa gctgcggcac agtgcgactg gttgcatctg
    27901 tttttagtca aagaagattc ttcacgcttc ccctatgaag accggctgga tctggtgtta
    27961 aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata catcatctcc
    28021 cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca ttgttacacc
    28081 gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg cactgggtgt aactcaccgc
    28141 tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga tatgcgctac
    28201 tggctgaaaa cgccgactat ctccgcaccg cccatcgaac tggttgaaat tgagcggctg
    28261 cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc gaaaaacgat
    28321 ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca gaacctgctt
    28381 gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa
    28441 aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat gtgatgatac
    28501 gcatcgcccc actcgatacg caggatatcg acctgcaaat caacagcagc gttgagaaac
    28561 agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac aacgtgcgcg
    28621 gcgtacagct gaatgtcgat gacaaaggca cactggactg cattttacgt gcacgactgg
    28681 aagccctgct ggcacgcgcc agcggtatcc cgactctgcc atgggaggat tgccaatgat
    28741 ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg
    28801 tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc tgatgtttga
    28861 cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg tttatcacgc
    28921 gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtcaacg cgctggattc
    28981 cgaatggggt gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct
    29041 gccgaaaacc gataccgctc aggatgttct ggatattgaa aaagagatcc tgcgtatcga
    29101 aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg aatctccgct
    29161 gggcattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg gtatcgccct
    29221 cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag gaactgaact
    29281 gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga
    29341 taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg cccacatcaa
    29401 acagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc tgctgcacaa
    29461 cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga
    29521 agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc
    29581 ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga
    29641 ataaggcaat caaaatgacg cagaaaattg aacaatctca acggcaagaa cgggtagcgg
    29701 cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca gaactcacca aagcaaacct
    29761 accaggctga aaaagcgcgc gatcgcaaac tgtgtgccaa cctggaagaa gcgattcatc
    29821 gctctggttt acaggacggc atgacggttt ccttccatca cgctttccgt ggcggtgacc
    29881 tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac ctgactctgg
    29941 cctccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc cagggcgtgg
    30001 ttacccgcat ttatacctct ggcctgcgcg gtccactggc ggaagaaatc tcccgtggtc
    30061 tgctggcgga accggtgcaa atccactctc acggcggacg tgtgcatctg gtacagagcg
    30121 gcgaactgaa tatcgacgtg gctttcctcg gcgtcccatc ctgtgatgaa ttcggtaatg
    30181 ccaacggtta caccggtaaa gcctgctgtg gctccctcgg ctatgcaatg gttgatgccg
    30241 acaacgcaaa acaggtcgtg atgcttaccg aagaactgct gccttatccg cataatccgg
    30301 caagcattga gcaagagcag gttgatttga tcgtcaaagt tgatcgcgtt ggcgatgctg
    30361 caaaaatcgg cgctggtgcg acccgtatga ccactaaccc gcgcgaactg cttattgccc
    30421 gtagcgctgc ggatgtgatt gtcaactctg gctacttcaa agaaggtttc tccatgcaaa
    30481 ccggcaccgg cggcgcttcg ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc
    30541 gcgatattcg cgccgacttc gccctcggcg gtattactgc aacgatggtt gatctgcacg
    30601 aaaaaggtct gatccgcaaa ctgctggatg tgcagagctt tgacagccat gctgcgcaat
    30661 cgctggcccg taaccccaat cacatcgaaa tcagcgccaa ccagtacgct aactggggtt
    30721 cgaaaggcgc atcggttgat cgtctcgacg tggtggtact gagcgcgctg gaaattgaca
    30781 cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt actgcgtggt gcttccggtg
    30841 gtcactgcga taccgcgatt gcctctgcgc tttccatcat cgtcgcgccg ctggtacgcg
    30901 gtcgtattcc gactctggtg gataacgtgc tgacctgcat caccccgggc tccagtgtcg
    30961 atattctggt cacagaccac ggtatcgcag ttaacccgac acgtccggag ctggcagaac
    31021 gtctgcagga agcgggcatt aaagtggttt ccattgagtg gctgcgtgaa cgtgcgcgtc
    31081 tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt
    31141 accgcgatgg ctcggtgatc gatgttgtac atcaggtgaa ggaataagcc atgcacctgc
    31201 ttcctgaact cgccagccac catgcggtgt caattcccga gctgctcgtc agccgggatg
    31261 aaaggcaagc acggcaacac gtctggctca agcgccatcc tgttccactg gtctccttta
    31321 ccgtggttgc gcctaggccg attaaagaca gtgaggtcac acgccgaatt tttaatcatg
    31381 gcgtgacagc cttgcgtgcc ttagccgcaa aacagggctg gcaaattcag gagcaggctg
    31441 cactggtttc cgccagcggg ccggagggta tgttgagcat tgccgccccg gctcgcgacc
    31501 tcaagctcgc caccattgag cttgaacata gtcatcctct cgggcggtta tgggatatcg
    31561 atgtcctgac gcccgaaggc gaaattctct cccgccgcga ctattcactg ccgcctcgcc
    31621 gctgcctgtt gtgcgagcaa agcgcagccg tctgcgcgcg tggaaaaccc atcaactgac
    31681 cgatttactc aaccgcatgg aggcactgct gaacgatgtc gatgcctgca acgtcaacta
    31741 aaaccacaaa gcttgcgacg tcattaatcg atgagtacgc cctgctgggc tggcacgcca
    31801 tgctgactga agtcaatctg tcaccgaaac caggtctcgt ggatcgcatt aactgcggtg
    31861 cgcataaaga tatggcgctg gaagatttcc accgcagcgc gctggcgatt cagggctggc
    31921 taccccgttt cattgaattt ggtgcctgta gtgcggaaat ggcaccagaa gcggtactcc
    31981 acggattacg cccaattggt atggcttgcg aaggtgatat gttccgcgcc accgcgggcg
    32041 taaacacgca taagggcagc attttttctt tagggctact gtgtgcggca attggccgtt
    32101 tgcttcagct caaccagtcg ctaacgccaa caaccgtttg ttctacggcg gcaagtttct
    32161 gccgtggcct gaccgatcgc gaactgcgta ccaataattc acaactgacg gcaggtcaac
    32221 ggttgtacca gcagcttgcc cttaccggcg cacgcggtga agccgaagcg ggttatccac
    32281 tggtgatcaa ccacgccttg ccgcattacc tcactctgct ggatcagggg ttagatcctg
    32341 aactggcatt gctcgatacc ttgctcctac tgatggcgat caacggcgat accaacgttg
    32401 cttcgcgcgg tggcgagggg gcctgcgctg gctacagcgc gaggcgcaaa cattattgca
    32461 aaaagggggc attcgaaccc ccgccgatct cgattatctc cggcagttcg acagggagtg
    32521 tatcgaacga aatctcagtc taggcggcag tgccgacctg ctgatcctta cctggttttt
    32581 agcacagatt taattattta agcacttgat aaatttggaa atattaattt tcggagaacc
    32641 cgtatgtctt tagcaaaaga taatatatgg aaactattgg ccccactggt ggtgatgggt
    32701 gtcatgtttc ttatccctgt ccccaacggt atgccaccac aggcatggca ttacttcgct
    32761 gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa ttccggcaac agcgatcagt
    32821 tttattgcgg ttactatttg cgttattggc agtaattacc tgctctttga tgccaaagaa
    32881 ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga aatggggtct ggctggtttt
    32941 tccagcacca cggtatggct ggtatttggc gcatttattt ttgcattagg gtatgaagtt
    33001 tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat tcatgggcaa acgcacgctg
    33061 acgttgggtt atgcgattgt cattatcgac attctgctgg cgccgtttac accgtccaac
    33121 accgcgcgta ccgggggtac ggtttttccg gtcattaaaa acctgccgcc gttgtttaaa
    33181 tcattcccca acgatccgtc cgcgcgtcgt attggcggct atttgatgtg gatgatggtc
    33241 attagtacca gtctgagttc gtccatgttt gtcaccggtg cggcaccaaa cgtgctgggt
    33301 ctggagttcg tcagcaaaat tgccggtatc cagattagct ggttgcagtg gttcctctgc
    33361 ttcctgccgg ttggggttat cttgcttatc attgcgccgt ggctttccta cgtgctgtac
    33421 aaaccggaaa tcacacacag tgaagaagtg gcaacctggg cgggtgatga actgaaaacc
    33481 atgggtgcgc tgacacgcag agagtggaca ctgattggcc ttgtattgct cagcttaggt
    33541 ttgtgggttt ttggcagtga agtcattaat gctactgcag ttggtctgct ggcagtttcg
    33601 ctaatgctgg ccctgcacgt tgtgccttgg aaagatatta cccgctataa cagcgcatgg
    33661 aacacgctgg tcaacctggc aactctggtt gtgatggcta acggcctgac tcgttctggc
    33721 tttattgact ggttcgccgg taccatgagt acgcacctgg aaggattctc accaaacgca
    33781 acggtgattg tactggttct ggtgttctac tttgcacact acctgtttgc cagcctgtct
    33841 gcgcacaccg caaccatgct gccggttatt ctggccgtcg gtaaaggtat tccgggcgta
    33901 ccaatggaac aactgtgtat cctgctggtg ctgtctatcg gtatcatggg ctgtctgacg
    33961 ccctatgcaa ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat
    34021 tactggcgtc ttggcgcaat cttcggggtg atttatatct ctatgttgct gttggttggc
    34081 tggccgattc tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc
    34141 ccgccaggta agcccacctg gcgggcgtga tgatttaata gatagtattc agtgtatgta
    34201 tgagttccac acccattatg aaagcattct ggcgtaacgc cgcgttgctc gcggtttctc
    34261 tgcttccctt ctcttctgcc aacgccgtag cgttgcaggc aaaacagtat ggcgattttg
    34321 atcgctatgt cctggccctc tcctggcaaa ccggattttg ccagagtcaa cacgatcgaa
    34381 atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac gaccaacaaa gctgattttc
    34441 tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt tgctgcccgt ggtgttgatg
    34501 aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat cccgaatcta ccagaagcgc
    34561 acgccagccg gatgtgttca tcgccggaaa ccgggttatc actggaaacg gccgctaaac
    34621 taagtgaagt catgccagga gctggcggac gttcctgcct ggaacgctac gaatatgcca
    34681 aacacggtgc ctgctttggt tttgatccgg acgcatactt cggtacgatg gtacgcctga
    34741 atcaagaaat taaagagagc gaagccggaa aattccttgc ggataattac ggtaaaacag
    34801 tgagccgccg tgactttgac gccgcctttg ccaaaagctg gggaaaagag aacgtgaaag
    34861 cagttaagct aacgtgccag ggtaaccctg cgtatttgac tgaaattcag atctcgatca
    34921 aagctgacgc catcaacgct ccgctttctg caaactcatt cttgccacaa ccacacccaa
    34981 gcaactgtgg caaaaccttt gtgattgata aagtgggtta ttaatcatct gatttacaaa
    35041 acacgctaat tcgtc
//
LOCUS       DAJVOY010000021        34989 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVOY010000021 DAJVOY010000000
VERSION     DAJVOY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.021"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            8..1246
                     /gene="dsdA"
                     /locus_tag="OQL90_000923"
                     /pseudo
     CDS             8..1246
                     /gene="dsdA"
                     /locus_tag="OQL90_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(1354..2892)
                     /gene="emrY"
                     /locus_tag="OQL90_000924"
     CDS             complement(1354..2892)
                     /gene="emrY"
                     /locus_tag="OQL90_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1367784.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2892..4055)
                     /gene="emrK"
                     /locus_tag="OQL90_000925"
     CDS             complement(2892..4055)
                     /gene="emrK"
                     /locus_tag="OQL90_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1367785.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4471..5085
                     /gene="evgA"
                     /locus_tag="OQL90_000926"
     CDS             4471..5085
                     /gene="evgA"
                     /locus_tag="OQL90_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1367786.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGASAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5090..8683
                     /gene="evgS"
                     /locus_tag="OQL90_000927"
     CDS             5090..8683
                     /gene="evgS"
                     /locus_tag="OQL90_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1367787.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8739..9884)
                     /gene="yfdE"
                     /locus_tag="OQL90_000928"
     CDS             complement(8739..9884)
                     /gene="yfdE"
                     /locus_tag="OQL90_000928"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1367788.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9958..10902)
                     /gene="yfdV"
                     /locus_tag="OQL90_000929"
     CDS             complement(9958..10902)
                     /gene="yfdV"
                     /locus_tag="OQL90_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1367789.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10972..12666)
                     /gene="oxc"
                     /locus_tag="OQL90_000930"
     CDS             complement(10972..12666)
                     /gene="oxc"
                     /locus_tag="OQL90_000930"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1367790.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12720..13970)
                     /gene="frc"
                     /locus_tag="OQL90_000931"
     CDS             complement(12720..13970)
                     /gene="frc"
                     /locus_tag="OQL90_000931"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1367791.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14483..15115)
                     /locus_tag="OQL90_000932"
     CDS             complement(14483..15115)
                     /locus_tag="OQL90_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1367792.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15411..15686
                     /locus_tag="OQL90_000933"
     CDS             15411..15686
                     /locus_tag="OQL90_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367793.1"
                     /translation="MKVNLILFSLFLLVYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15763..16005)
                     /locus_tag="OQL90_000934"
     CDS             complement(15763..16005)
                     /locus_tag="OQL90_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1367794.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16358..17278
                     /gene="lpxP"
                     /locus_tag="OQL90_000935"
     CDS             16358..17278
                     /gene="lpxP"
                     /locus_tag="OQL90_000935"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1367795.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17634..17705
                     /gene="ypdK"
                     /locus_tag="OQL90_000936"
     CDS             17634..17705
                     /gene="ypdK"
                     /locus_tag="OQL90_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1367796.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17770..19008)
                     /gene="alaC"
                     /locus_tag="OQL90_000937"
     CDS             complement(17770..19008)
                     /gene="alaC"
                     /locus_tag="OQL90_000937"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1367797.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19385..21081
                     /gene="ypdA"
                     /locus_tag="OQL90_000938"
                     /pseudo
     CDS             19385..21081
                     /gene="ypdA"
                     /locus_tag="OQL90_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
     gene            21096..21830
                     /gene="ypdB"
                     /locus_tag="OQL90_000939"
     CDS             21096..21830
                     /gene="ypdB"
                     /locus_tag="OQL90_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1367798.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21843..22700
                     /locus_tag="OQL90_000940"
     CDS             21843..22700
                     /locus_tag="OQL90_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1367799.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22703..25198)
                     /gene="ptsP"
                     /locus_tag="OQL90_000941"
     CDS             complement(22703..25198)
                     /gene="ptsP"
                     /locus_tag="OQL90_000941"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1367800.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25223..26260)
                     /gene="ypdE"
                     /locus_tag="OQL90_000942"
     CDS             complement(25223..26260)
                     /gene="ypdE"
                     /locus_tag="OQL90_000942"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1367801.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26260..27345)
                     /gene="ypdF"
                     /locus_tag="OQL90_000943"
     CDS             complement(26260..27345)
                     /gene="ypdF"
                     /locus_tag="OQL90_000943"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1367802.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27360..28607)
                     /locus_tag="OQL90_000944"
     CDS             complement(27360..28607)
                     /locus_tag="OQL90_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1367803.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(28629..28955)
                     /locus_tag="OQL90_000945"
     CDS             complement(28629..28955)
                     /locus_tag="OQL90_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1367804.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(29174..30139)
                     /gene="glk"
                     /locus_tag="OQL90_000946"
     CDS             complement(29174..30139)
                     /gene="glk"
                     /locus_tag="OQL90_000946"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1367805.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30343..31599
                     /locus_tag="OQL90_000947"
     CDS             30343..31599
                     /locus_tag="OQL90_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1367806.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            31714..32040
                     /locus_tag="OQL90_000948"
     CDS             31714..32040
                     /locus_tag="OQL90_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1367807.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERSYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(32181..33419)
                     /locus_tag="OQL90_000949"
     CDS             complement(32181..33419)
                     /locus_tag="OQL90_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1367808.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKTLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVLPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            33755..34957
                     /gene="nupC"
                     /locus_tag="OQL90_000950"
     CDS             33755..34957
                     /gene="nupC"
                     /locus_tag="OQL90_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1367809.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         9228 a         8688 c         8075 g         8998 t
ORIGIN      
        1 ttgaagtggc acgacctcat tggctgaagg tttaccttat gttggcctga ccgaacagga
       61 tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa aagcatttgc
      121 tgaaactgct gccactgggg ggattattga atcagaactg gttgccattc cagctatgca
      181 aaaacggctg gaaaaagaat atcagcaacc gatcagcggg caactgttac tgaaaaaaga
      241 tagccatttg cccatttcca gctccataaa agcacgcggc gggatttatg aagtcctggc
      301 acacgcagaa aaactggctc tggaagcggg gttgctgacg cttgatgatg actacagcaa
      361 actgctttct ccggagttta aacagttctt tagccaatac agcattgctg tgggctcaac
      421 cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta aggtgacagt
      481 tcatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc atggcgttac
      541 ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag gaaggacgta aagcagcgca
      601 gtctgacccg aactgtttct ttattgatga cgaaaattcc cgcacgttgt tccttgggta
      661 ttccgtcgct ggccagcgtc ttaaagcgca atttgcccag caaggccgta tcgtcgatgc
      721 tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg gtggcgtcgc
      781 attcgggctt aaactggcgt ttggcgatca tgttcactgc ttttttgccg aaccaacgca
      841 ctccccttgt atgttgttag gcgtccatac aggattacac gatcagattt ctgttcagga
      901 tattggtatc gacaacctta ccgcagcgga tggccttgca gttggtcgcg catcaggctt
      961 tgtcgggcgg gcaatggtgc gtctgctgga tggcttctat acccttagcg atcaaaccat
     1021 gtatgacatg cttggctggc tggcgcagga agaaggtatt cgtcttgaac cttcggcact
     1081 ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta agttaccaac agatgcacgg
     1141 tttcagcgca gaacaactgc gtaataccac tcatctggtg tgggcgacgg gaggtggaat
     1201 ggtgccggaa gaagagatga atcaatatct ggcaaaaggc cgttaataac gtttcaacgc
     1261 agcatcgcaa tcctttccct gggtgagcga tgctgccgat ggcgcagact taagatcccc
     1321 ggtcttaccc gctataaccc cctcctttta tcatcaccca acgcctttcg ctgtaaacgg
     1381 cggtttcgca aaccaaacca aaaccgtaag taagataaaa gcaatagccg ccatacggaa
     1441 aatctcattt gcagaaatag aaagtgactg ctgggttatt tcattattaa tttcattaag
     1501 aactcctgaa agcgaaccat aatatttatc cataatttgt gacgatgaat taaacacggg
     1561 gttaaattga tcgatggttg ctgtcaactg actatggtgt aacgattcgc gtcgtcccca
     1621 cagcgtcatt gtcaacgacg taccaactga tcctgacaag gtacgaaaaa aattactcat
     1681 actcgaggca ttggcaaatt tattatctgg caagcctgaa aacgaaatcg ttgttaaggg
     1741 taaaaagaaa caggcaacgg cgaatccctg aaaaaactgc ggcaaaatga tgcctgtaaa
     1801 atcaatcgtt ggcataaatg tcacagaacg ccagtaatag caaaccgcat acatcaaaaa
     1861 actaaatgtc actaacaacc gcatgtctat tttgttgcca taacgtccta tcaaaggtga
     1921 aattaatagt ggcatgatgc cgatgggcgc ataagcaagt ccggcccata tcgcattata
     1981 ccccatcgtt tcctggagta actgcggcat aaggacgatc gctccagagt aaaataaata
     2041 cgcgcatgtg atactcacaa taccaatggt gaagttacgg gacttaaaca aactgagatc
     2101 aagaatcggg ttctctgagg tcgactccca aatgactaaa gagatcagag aaataactga
     2161 tactactgtt aatattatta ttgtactcga gttgaaccaa tccagatcgc gccctttatc
     2221 aagcataatt tgcaagccac caacaccgag cactaacagg gtcagtcctg gtagattcat
     2281 tttgaccggt gaagtctcag tttctcttcc tttaagtaag gttaagcata atgtcaggac
     2341 gataatcccc atagggacat tgattaaaaa tatccaaccc cagctaaagt tatcacaaat
     2401 ataaccgccc aatatcggcc cacatatcgg agcgataatc acggtcattg accataatgc
     2461 cagagcaaat gttctttttt ctggcggata attccttaat aataaactct gtgacagtgg
     2521 aattaacggc cccgccatta acccctgaac gactctaaaa aatatcagca catcaagatt
     2581 ggtcgatagg ctacacatta atgaagacag cgaaaaaaag gttactgaaa gtaaaaataa
     2641 tcttaattcg cctattcttt gtgccaacct gccagtaaca gggatcgcaa tggcatttgc
     2701 tacaccaaac gaggtgataa cccaggtgcc ttcgtctgtt gatgctccca gaaagccaga
     2761 tattgtcggt attgcgacgt tagaaatagt ggaatccaac atttgcataa atgtcgctaa
     2821 tgacaatgca atagtgacgc accataacgt cccaccggtt aatggtgccg gagtcgattt
     2881 agtgattgcc attaaagttg tccattatgc gaaataatgt tgctaatttc tttttctatc
     2941 ggactggtat cgataactaa agccttactg gtataagccg gcatggaggt cacggttgaa
     3001 gccagctcag gcatctcggc aatgtcttcg ttcttcgtat caatagttgc tgtcatcgat
     3061 aaaccaatac gcaagggatg ttccatgagt tcttttggat caagagaaac ttcaaccggt
     3121 acacgctgaa cgattttgat ccagttccct gtcgcatttt gtgcaggtaa taaggagaac
     3181 gcattgccgg ttcccatatt gatccctgtc acccgaccat gaaacacaac attttcacca
     3241 taaagatcgc tgataatatt gaccgattga ccaatccgca catccgtgag ttgtgtttct
     3301 ttaaagttgg cattaaccca catttgacgt gccggtacga cagccattaa cgattgtccg
     3361 gggctcactg tttcgccgac ctgaacactt ctctgggcaa tatagccggt aaccggactc
     3421 ttaatatccg tacgtttaag cgccaaccag gcttctttag ttgcatccgc cgcttcaacg
     3481 acttgtggct gacggtttaa tggtgtgttc attactaaag ctttattcgc tttataagcc
     3541 tggatagcgg cattcaatgc cgctttgcta cttattaacg tatctttggt atgctccagc
     3601 gtttcttttg aaataacccc ctgcttcgct aacggcactc gacggttata atcttctaaa
     3661 gattgttgat actgaatacg tgctgaagcg acttcggcac tgtattgttt atcctgtaag
     3721 tatagtttat tcgtttgccg aacaatattt gccagattat ttttagcttt attgagtgcg
     3781 atagtggcat cagttttatc cagtgaaact aaaatgtcac cttgtcgaac gtagttcgta
     3841 tctttatgat taacgacagt gacactacct gagacttgtg cagaaattgg atctgcattc
     3901 cccgtgacat aggcgtcatc tgtactaatc atgtcttcta attccattga ccaatagaca
     3961 taggcacctg aaaacgcaat aaataaaact accgccaata aagaaaagta ttttcttctg
     4021 ttagaatgtt ttttatttga attaatctgt tccactatta tctctcattt ctcatagatg
     4081 aaatttatga attgaatgaa aagagcattc attcttcata tcaaacacag tgcatgtgtt
     4141 gcacattatc ccaggaacgt tggaggccta atttaaatga ggtgcaaaaa aaacaacaga
     4201 ggactaaacc gtggcttttg caatacaatt cttacgcctg taggattagt aagaagactt
     4261 atagtgctaa cttgaaacta taaatcatcg gtacaacccc tgattttatt gttgacattt
     4321 catttatgcc gactatttat atggtatact tgtcgaatta tcttaaagga agctcagatt
     4381 ttcttatttt tattgagaaa atgagatgac gccttatgtc tgtattacta cagggagaag
     4441 ggagatgctt cattgcaaag ggaataatct atgaacgcaa taattattga tgaccatcct
     4501 cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat cttagcggag
     4561 ttgactgaag gggcaagcgc cgttcagcgg gtggaaacac ttaagcctga tatcgtcatc
     4621 attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct gaggaagcgc
     4681 caatatagcg gaattattat tattgtctcc gctaaaaatg accattttta cgggaaacat
     4741 tgtgctgatg ctggcgctaa tggattcgtg agtaaaaaag aaggcatgaa caatatcatt
     4801 gcggctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct caaccggttt
     4861 gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca agaaattagt
     4921 gtcatgcggt atattcttga tggcaaggat aataatgaca ttgctgaaaa aatgttcatc
     4981 agcaacaaaa ctgtcagcac ttataaaagt cgcctgatgg aaaaattaga atgtaaatca
     5041 ctgatggatc tttacacatt cgcgcaacgt aacaaaatcg gctaatcaca tgaagttttt
     5101 accctatttt tttcttctct gttgtggtct ttggtcgacc ataagtttcg cagacgaaga
     5161 ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc cagtacgtct
     5221 gagcaacaag gaattacgtt ggttagcgag caaaaaaaat cttgtgattg cagtacacaa
     5281 gtcccaaacg gctaccttgt tgcataccga ttcgcagcaa cgggttcgtg gtattaatgc
     5341 tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc gggaatacgc
     5401 cgatcatcaa aaagcaatgg acgcgctgga agaaggtgaa gtcgatatag tgttatcaca
     5461 tttagttgct tcgccgcctc ttaatgatga cattgctgca accaaaccac tgataattac
     5521 ctttccggcg ctcgtcacca cccttcacga ttcaatgcga ccgcttacct catcaaaacc
     5581 agtaaatatt gctcgagtag caaattatcc ccctgacgag gtaattcatc aatcatttcc
     5641 aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg tctcagccgg
     5701 acagaatgat tactttattg gtagtaacat cattaccagc agtatgattt cccgctattt
     5761 cactcactcc ttaaatgtag tgaaatatta taactcaccg cgtcaatata attttttctt
     5821 gaccagaaaa gaatctgtcg ttcttaatga agtactcaat cgatttgttg atgctttaac
     5881 aaatgaagtt cgctatgaag tttcacaaaa ttggcttgat acaggaaacc tggcctttct
     5941 gaacaaacca ttagaactca ctgaacatga aaaacagtgg attaagcaac atcccgattt
     6001 aaaggtgctg gaaaatcctt actctcctcc ctattctatg acggatgaaa atggctcggt
     6061 tcggggcgtt atgggggata ttcttaatat tattaccttg caaacaggtt taaatttttc
     6121 tccgatcacc gtttcacaca atatccatgc cggaacacag cttaaccccg gtggatggga
     6181 tataatacct ggcgctattt atagtgaaga tcgagaaaat aatgtttcat ttgcagaagc
     6241 tttcataaca acgccatacg tttttgtcat gcaaaaagca cctgacagtg aacaaacatt
     6301 aaaaaaagga atgaaagttg ccattccata ttattatgag ctgcattcgc aattaaaaga
     6361 gatgtatccg gaggttgagt ggataaaagt cgataacgcc agcgctgcat ttcacaaggt
     6421 caaggaaggt gaacttgatg ctctggtcgc gacacagcta aattcgcgtt acatgatcga
     6481 tcattactat cctaatgaac tgtatcattt tcttattccc ggcgttccga atgcatcgct
     6541 ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata aagcactgaa
     6601 tgcaattccc ccaagcgaag ttctgcgcct gacggaaaaa tggattaaaa tgcccaatgt
     6661 gaccattgac acatgggacc tctatagcga gcaattttat attgttacga cattatccgt
     6721 tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag ttcgtcgtcg
     6781 taaagtcatt cagggtgatt tagaaaacca aatatcattc cgaaaagcac tctcggattc
     6841 cttaccaaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc ataatagtgc
     6901 ttttgaacat tatttcactg cggattacta caaaaatgca atgttaccat tagaaaacag
     6961 tgactcaccc tttaaagatg ttttttctaa tgcgcatgaa gtcacagcag aaacgaaaga
     7021 aaatcgaaca atatacacac aggtatttga aattgataat ggcatcgaga aaagatgcat
     7081 taatcactgg catacattat gcaatctgcc agccagcgat catgctgttt atatttgtgg
     7141 ttggcaagat attaccgaga cgcgtgattt aattcatgca ctcgaagtag agagaaataa
     7201 agcgatcaat gcaactgtcg caaaaagtca gtttctggca acaatgagtc acgaaataag
     7261 aacaccaata agctccatta tgggcttcct ggaacttctg tcgggttctg gtcttagcaa
     7321 ggagcaacgg gtggaggcga tttcacttgc ctacgccacc ggacaatcac tcctcggctt
     7381 aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac ttcaaccaca
     7441 atgggtcgat atccctactt tagtccagaa cacttgtcac tctttcggtg cgattgctgc
     7501 aagcaaatcg atcgcattaa gttgcagcag tacatttcct gatcattacc tggttaagat
     7561 cgaccctcag gcgtttaagc aggtcttatc aaatttactg agcaatgctc tcaaatttac
     7621 caccgagggg gcagtaaaaa ttacgacctc cctggttcac attgatgaca accacgctgt
     7681 tatcaaaatg acgattatgg attctggaag tggattatcg caggaagaac aacaacaact
     7741 ctttaaacgc tacagccaaa caagtgcagg tcgccagcaa acaggatctg gcttaggctt
     7801 aatgatctgc aaagaattaa ttaaaaatat gcagggcgat ttgtcattag aaagtcatcc
     7861 aggcatagga acaacattta cgatcacaat cccggtagaa attagccagc aagtggcgac
     7921 tgtcgaggca aaagcagaac accccatcac actacctgaa aagttgagca tattaatcgc
     7981 ggatgatcat ccgaccaaca ggctattact caaacgccag ctaaatctat taggatatga
     8041 tgttgatgaa gccactgatg gtgtgcaagc gctacacaaa gtcagtatgc aacattatga
     8101 tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga ctcgcaaact
     8161 ccgtgagcaa aattcttcct tacccatctg ggggcttaca gccaacgcac aggctaacga
     8221 acgtgaaaaa gggttaagtt gcggcatgaa cttatgtttg ttcaaaccgt tgaccctgga
     8281 tgtactgaaa acacatttaa gtcagttaca ccaggttgcg catattgcac ctcagtatcg
     8341 ccaccttgat atcgaagccc tgaaaaataa tacggcgaac gatctacaac tgatgcagga
     8401 gattctcatc actttccagc atgaaacgca taaagatcta cccgctgcgt ttcaagcact
     8461 agaagctggc gataacagaa ctttccatca gtgtattcat cgcatccacg gtgcggctaa
     8521 catcctgaat ttgcaaaagt tgattaatat tagccatcag ttagaaataa cacctgtttc
     8581 agatgacagt aagcctgaaa ttcttcagtt gctgaactct gtaaaagaac acattgcaga
     8641 gctggaccag gagattgctg ttttctgtca gaaaaatgac taaatagcgg ctcccacaat
     8701 gttcaaatgt gggagctatt taccagcaca tcttacattt atgatgagaa ctcctggcga
     8761 atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg gtccgcgcag
     8821 ccgctgattt ttatcggatt acccggcatc attattcccc cggcttcaat caacatattt
     8881 ctcgcctgag tttgtggcaa tttaatggcc tcagccacac ttaataacgg cgcgacgggt
     8941 acaccaactt catgtattct ggctaaccaa acttcagctg cctgcgtttt taacgtccgc
     9001 tcaatatatt gtttaagaat agcctggttt tgtacgcgta aaatattgct gctaaatcgg
     9061 ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagaaaa aagcttgtca
     9121 ttaccacaac aaatcgtaat cggcttatcc tgagtattga aaacatcaaa aggtgccatg
     9181 tagggatggc gatttcccag acgttgtggt gacttccctg tcgcgatata tgccatcaga
     9241 ccatgctcca gaaaactcag cgtggcatca aacatcgcta tatcgacatg cgcccctctc
     9301 tggctctttt cgcggccata aagtgcactc actattccgc tgaataaata gacaccgccg
     9361 catagatccg caagagatgt accaacgcgc actggcggag catcaggata tcctgtttcc
     9421 atcattatcc cgctcattgc ctgaatgatg gtatcgtagg caggagcatc ttttagcgga
     9481 ccggtatgtc cgaaacctga cgatgaagca tatatgaggc gcgggttgat ttcttgaagc
     9541 gtttcccatg aaaaccccag tttttccatt gtacctgggc gaaaattctc agctaataca
     9601 tcagcttgtt tgagcatatt tataaatata cttttatcgt aatcattctt taaatcaaga
     9661 accacactct ctttgccatg attaataaaa ctgtaataga gtgactgtcc atccacatag
     9721 ggaccaaatg tgcgggtatc atcaccatga cccggcggct caactttaat tacccttgcg
     9781 cccatattac aaagaagttg agttccgaaa ggtccattaa ggacatgtgt catatcgata
     9841 actaataagc cttcaaacgg ccctttgctt tcattatttg tcatttgctt atgccttgaa
     9901 aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca tgattaatta
     9961 tgaaaccagt cgactgacat aaatccacaa gggagccgtg acgacaaagc ccagtacgct
    10021 cacagccaat gacgctgtac cagtacgggt gtagacatta aaccggctgg caataataat
    10081 tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcagat gttcgctgtt
    10141 caaatgacat gccataccaa cgagaagcag tgccagtggc atcagaatca gcttcaggaa
    10201 ggtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag tcaaccccgc
    10261 agcgaatacc gctacccctg agttagcctt cgcaatcaga ttaaaggttg ggtcccatgc
    10321 tgccggaatt tttaccccaa ccaacaccag gatcgttgcc agaacaggtg cccatactac
    10381 tggctccttt gccgcagaaa ttaatgcact cagattacta ttcttcttac catccgctcc
    10441 tgaagaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa caataataga
    10501 aataattgct accactaaac ctgttgatac ggaatcacca taaataggat cgagaactgc
    10561 aaaccccagg aatccaatgg taggtgaacc tgcaattaat gcacatacag ctgcttctgc
    10621 atgggtacgt ttaaaaaatt tgtagcaacc gaaccaggag aaaaagaaac atccgacaat
    10681 aaccacaagt gatacaaggg tcagacgagt gtccgcaaaa atcatttccc tgtttgcccg
    10741 agtaatagat acaaatagag ccgcaggaag cgcgtagttt aataccagtt tattaaatgc
    10801 gcgggcttga tcttctgaaa aagtttctcg tctgccgcta aaataaccca acagcatgat
    10861 tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat ttccgataag
    10921 ggtcccctca ccatcgcata atgagttggc aagaggagac gatgtcagta tttaattacc
    10981 agcgacttgt tttgggttaa gtttggtaat atggccactt tcagttcctg ctgcagggtc
    11041 gatgaccaca ttaataatgg tcggtttgcg cgactgaata ccggtggtta aagcatgacg
    11101 aagttcatct gtcgtggtga cgttatagcc aacgccacga aacgcatcca ttaatttgtc
    11161 ataccttgca tggtgcaaca gatccgttgg agatggtgca ccagcgccac tgagatcaac
    11221 accgtctcct ctgtagatgc cgccattatt aaaaataacg atcgtcaccg gcaggttata
    11281 tcgacaaatc gtttcaattt ccatcccact gaaaccaaaa gcactatcac cttcaatggc
    11341 gacaaccgga gaaccagaag tcacgctagc accgatggca tagcccatac cgatgcccat
    11401 gacaccccag gtaccacaat ccagacgacg acgtggttta tacatatcaa taatatttcg
    11461 tgcattatcc agggtatttg caccttcatt aactaaataa atatcctggt tctcgcgcaa
    11521 tacgtcgcgc acagaactta atgcattaaa gtaatttaat ggttgagtat ctgtacttaa
    11581 tttttcatgc attttttgtg cattttgctg cttgtggata tttaaaatat cgcgccatac
    11641 cagtggagtc gtaaatgtgt tttgtttcag ttctgccagc ataccttgca tactggatgc
    11701 aatatcacca atgactggca cagcaatggg gcggttgctg tcaatttcct gcggttcaat
    11761 atccagttga ataaactgtg tatctgccgc ccatcctttt ttaccgtgtg ccaataacca
    11821 attcagtcgt gcaccaacaa gcatgacaac gtcagcattt gccagggcaa acgaacgcgc
    11881 agctgccgca gaaagtggat gcgtatcttc aaggatccct ttcgccatag acattggcag
    11941 gaatggaatc tgagcacttt caataaattc acgaagctgt tcatcagctt gtgaatacgc
    12001 cgcgcctttg ccaaggataa ttaatggccg ttcagctttt gctaaaagcg aaattgcgct
    12061 agtgactgac ttcgggcatg gcaataatgc tggcgacgga ttttcaactt taacaatcgt
    12121 ggttaacgct tcgtcttttt ccatcgtcgc ggccaggaca tttgctggca aatcaagata
    12181 aactccgcca gggcgacccg atacagagac ccggatagcg cgtgccaatg caatgccaag
    12241 atcctgcggc tgattaacgc gaaatgctgc tttggcatac ggttttgccg cattcatttg
    12301 gtccagctct tcataatcac cttgctgtag gtcgacgatc gcgcggtcgc tggagccgct
    12361 aatcatgatc atcggaaaac catttaccgt tgcgttggcc aatgccgtca aaccattgag
    12421 gaatcctggc gcagaaactg tcaggcagat ccccggtttt tgggtaagaa aaccgcttgc
    12481 cgcagcggca tagcctgccg actgctcatg acgaaaacca atataacgaa tgccttccgc
    12541 ctgggcatgg cgtgccatat ccgtcacagg aatacctaca acaccataaa tagtgtcaat
    12601 attattctgt tttaatgctt caacgatgat atgcatacca tctgtcattt gaagttgatc
    12661 tgacatgatt aataacctcg aaatttttat aaattgggag ccagaagccc ccgttaatat
    12721 cagatggcgt ggttttgctt cattgcagca atttcatcgt cgctataacc tagctcctgc
    12781 aatacagcag cggtatgttc acctaatagc ggcgcagctt taatatccgg cgtaaaagca
    12841 gagaatttca ttggacaacc aacggtcaga tattttccac gcaacggttg ttccacttca
    12901 acaacactgc cactttggcg caaagaggga tcaagtgaaa tttctttcat acttaaaacc
    12961 ggtgcacaag gaatatcaaa ctgagtcaaa taggccaccg cttcatgttt atcaatagtg
    13021 acagtgtatt tttcgatttc agcaaaaata tcgaaaatat gtggctgtcg ggcatgggct
    13081 gtactgtatg ccggatcggt aatccattct ggtttgccga tggctttaca ggtgttttcc
    13141 cagttttgct cctgaatagt gaaataaata taggcgttcg gatcggtttc ccagccttta
    13201 catttcagga tccagccagg ctgaccgcca ccacctgcat taccaccgcg gggaactgca
    13261 tcaccaaatg taccattcgg atactgcggg tattcttcca gataacccaa tttatcgaga
    13321 cgctgctggt cacgtaattt cacgcggcaa aggttcaata cggcatcctg cattgacatg
    13381 gtgactcgtt gcccacgccc cgttttttcg cgatgcagca aagcagcaag caaaccgatc
    13441 agcaaatgca ttccggtgtt gctgtcaccc aacgctgcag cgcttaccag cggcggacca
    13501 tcccaaaaac ccgtagtgga tgccgcgcca cccgctgcct gagcaacgtt ttcataggct
    13561 tttacattca cataaggcga acactcatca aaccctttga tcgaaccaaa aatcagacgt
    13621 ggattgattt cttgaatatg ctcccaggtg aagcccatgt gatcaatggc ccctggatga
    13681 aagttctcga ctaagatatc agcttcgcgg atcagctttt ccattacctc tttgccttcc
    13741 gctgttttgg tatttaactc aatagaacgt ttgttactgt taagcatggt gaagtaaagc
    13801 gcatcgatat caggaatatc tcgcagctgg tgacgcgtta cgtcaccaac gccgggacgt
    13861 tcaattttaa taacgtcagc gccaaaccag gccagcattt gagtacaaga tgggccagat
    13921 tgcacaccgg tgaaatcgag aactttaatt ccttgaagtg gagttgacat agcaatacct
    13981 attagttaat taaatatgtg ttaatttagc gtttatttaa ggcgtaatat cttctataaa
    14041 taacccgcaa aatatcgtcg tagctgcact cgatattttt aaaatcctgt atcaagtgct
    14101 gaaaatcatt tcatcgccac ttccaattcc tttctgcatg gtaagtagtg aggacgaaaa
    14161 gaagaatatg ataaatattg attcgtggta atcagagaga gtcgcgacat ttgtgatgtt
    14221 attcttacaa ctgtaagaag cgccggtgat ttacaagtaa gggtatgatt acgcttttta
    14281 aagcgtacca taaaacataa cataataaat gatttgtgat gacacgggat tattggggtt
    14341 tactttgagg aagataaaat gcgtgtttat agaaccgcga gcgccaaata attttatgac
    14401 cacaacctgt cgtattgtgg tcataaaaaa ttgtcggctg gagggagtta aatcctttac
    14461 ctcaatatac tgcctgtcag cattagttca caaacagtgc ttcgctgtcg acgatgattc
    14521 catcttccgc acctttcagc gccaggtttg cctcgtagta ttgcttttta tcaagcagtt
    14581 tctgcgcatc agcaagtgca ttacgagtct gtttaagcgg catcagatat tggttttcca
    14641 ttacgccaac accggccagg cgaagttctt ccattgcgcc ctttttatcc cctttcgcca
    14701 ttttttcatt ggcaattttt atcgcggctt ctttttcggg agttgctaca taactctcag
    14761 aaattccgac agatgcatta atgacaatat attggtcatc attgagattg gtctttttac
    14821 ctggcttagc gaactttgcc cattcggtac tgtcatctga cagcaaagcg gaagcttcat
    14881 tggtcagttc ttttgccttt tctggatcgc catgaaacag agccaggcgc gctacctgca
    14941 cgtcacgcat cgcatataaa ccttgttcag agatacgctc tgctgctgcc gttttctggg
    15001 tttgctgaac ttgctgagtt tgctgttgtg ccgctaccgg tgcattatca gcagcccata
    15061 cagttgaaga tgccagaatt gctgtgacca tcgtagccat aattaaacgt ttcatactta
    15121 cctcattctt taacagtttt agaaaacgcc gccatcatat tgggtgagca tgaaatgaga
    15181 aatcagagag caacggtcat tttgaaaggg ttatcttaca gttgtagtta ttatcgcaag
    15241 aagtatttta gtcttacaat tgtaggaatc tgcttcctgt cgcagagaac tattcttatg
    15301 taccacaaag agatgtcatt tattatatcg atgtaaatat tgattaatta tatgtaaata
    15361 cagccataac atcgtaggtt ctcagtgaac cattttcagg ataactttct atgaaagtaa
    15421 acttaatact tttcagctta tttttattgg tctatattat ggcatgcaat gtttttgcat
    15481 tttccatttc tggtggtgta agtgagagga gctataaaga gactgaaaaa acatcagcga
    15541 tgacgaccac acactctaca aaacttcagc catcacaggc gattttgttt aagatgagag
    15601 aagatgcgcc accattaaac ctcacagaag aaatcacgcc tacttatccg acaaaggcga
    15661 attatcttat tcatcctgtg cgatagcgtc tgtcacatgg caggtgaaca atcatgtctg
    15721 atgctgatgt gaacacctgc ccaaatgagc catcctgacc cactatcgcc tgccataccc
    15781 caccataaag cgaatcatca agccacaagc cggggcaaga aacataaata tctccgtcac
    15841 tagttcatta ccgccagtta accacatggt gaagtaataa atgagtgcag cgaggatcac
    15901 catgccgaag aaagaagaga ccgccgatac cacgttcagt acctgaccga tgtagccgct
    15961 tacgcagaca atacacaggg cgagaaacat ccataaataa atcatagctc aacctcctgc
    16021 cgcgctaaaa aacctgtaac ttcagtcact ttaatagtaa gacaatcctt aacctctggc
    16081 aatttttgct aagcatcaat gaaaacagca tgttaaatgc aagactgttg tgtacggaaa
    16141 aatatttact ttgcacgatt aataatcagt agctgaaagc agtcagcgag tgataaagta
    16201 cagataccgc attcatgctc ctcgtggtta tgtcctgact agtctttaca cactttacag
    16261 gaaccattgt cgtacatgat ggcccaacca attgagtgta ccgctgtcga catggaatct
    16321 tctgcgatac aacaattcgt atctacagaa ggtaactatg tttccacaat gcaaattttc
    16381 ccgcgagttt ctacatcctc gctactggct cacatggttt gggcttggtg tactctggct
    16441 ttgggtacag cttccttatc ctgttctctg ctttctcggc acgcgtattg gcgcaatggc
    16501 gcgaccattc ctgaaacgtc gtgaatctat cgcccgtaaa aacctggaac tttgtttccc
    16561 gcagcattct gcggaagaac gcgagaagat gattgccgaa aactttcgtt cactcggcat
    16621 ggcgctggta gaaaccggca tggcatggtt ctggcccgac agtcgcgtac gtaaatggtt
    16681 tgatgttgaa gggttggata accttaaacg cgcacaaatg caaaatcgcg gcgtaatggt
    16741 tgtcggcgtc cattttatgt cgctggaact gggcggccgc gtgatgggac tgtgccaacc
    16801 aatgatggct acctatcgtc cacataataa tcagctgatg gaatgggtgc agacccgtgg
    16861 gcgcatgcgc tctaacaaag cgatgatcgg cagaaataat ctgcgcggca ttgtcggtgc
    16921 actgaagaaa ggtgaagcgg tatggtttgc tcccgatcag gattatggtc gtaaaggcag
    16981 ctccttcgcg ccgttctttg cggtggaaaa tgtcgccaca accaatggca cctatgttct
    17041 ctcccgtctc tctggcgcag ccatgttgac cgtaacgatg gtaagaaaag cggattacag
    17101 cggatatcgt ttgttcatca ccccagagat ggaaggctac ccgacagatg aaaatcaagc
    17161 cgctgcctat atgaacaaga ttatcgagaa agagatcatg cgcgcaccgg agcagtacct
    17221 ctggatccac tgtcgcttta aaacgcgccc ggtgggagaa tcgtcgttgt acatttaaca
    17281 aactgctctt actctttccc tcctcaaatc ggccataata acctcatggt ttttatggct
    17341 tttttcttaa ggttttttcc ttcgctgaat atctaattat taccgtcaga aattaaactg
    17401 tcgccgatat gagacacttg taagttacta aaagtaaata agaaaatgcc tcttgtctcg
    17461 tttcagtttt aggcgtacat tagcgccgtc tcgagcagtt tagctcagaa ttctggggtg
    17521 atgcccacgg taaaatacag ggcttttaag gtcgctttgc ccagtgtcaa ctcactgtat
    17581 caggtgtaat gaagtcattc aggcgtaaca gcaattacgc ggagagatgt aaagtgaaat
    17641 atttctttat gggcatttct tttatggtca tcgtttgggc cggtactttt gccctgatga
    17701 tctaaaagca aactgtgcaa tgaaaaacag gagccatcag gctcctgttt gcatttctga
    17761 cgacggtgtt tattccgcgt tttcgtgaat atgtttgctg ctggcgggta gtaaaccgtc
    17821 ggcacggaac atcgctttaa tccctctgat ggcctgacga atacggtcgc ggttttcaat
    17881 cagtgcaaag cgaacatgcg tatcaccata gtcgccaaag ccaatccccg gcgagacaca
    17941 gaccttcgct tcgttaagca atttcttggc aaataccagc gagcccatgg cagcatacgg
    18001 ttccggtatt ttcgcccaga catacatcga agccttcggc atttcaacta tccagcccgc
    18061 ttcatgcagc cctttaacca atacatcacg gcggcgtttg tactgttcgg caatgtcgcg
    18121 cacgcactgt tgatcgccct ccagcgccgc aatcgccgcc acctgcaacg gcgtaaaggt
    18181 gccgtaatcg tgatagcttt taatacgagc cagggcgctg accagcgttt tgttgccaac
    18241 cataaagccg atacgccagc ccgccatgtt gtagcttttc gacagcgtaa agaactcgac
    18301 tgccacatcg cgtgcacccg gtacctgcat gattgacggc gctttccagc catcgtagac
    18361 gatatcggca taggccaggt cgtggaccac cagcacatcg tagcgtttcg ccagcgccac
    18421 aaccttttcg aagaactcca gctccacgca ttgcgcggtt ggattcgacg ggaagccgag
    18481 gatcatcatc ttcggtttcg gataactttc acgaatggcg cgctccagtt cgttgaagaa
    18541 atcgacacct tccaccagcg gcactgagcg tacctgcgcc ccggcaatca ccgcgccata
    18601 aatatggatc gggtaacttg gattcggcac cagcaccgtg tcaccatgat ccagcgtcgc
    18661 cagcatcaga tgcgccaggc cctctttcga accaatagtg acgatggctt ctgattccgg
    18721 gtcgatttca acgtcgtagc gatcctgata ccagcgggaa atggcgcgac gtaaccgcgg
    18781 aatgccgcgt gaagtggagt aaccatgcgt gtcctggcgc tgggccacag tacataattt
    18841 ttcgacgata tgcggcggag tcgcaccgtc cgggttaccc atgctgaaat cgataatatc
    18901 ttcgccgcgc cgacgcgcag ccattttcag ttcagcggtg atgttaaaaa cgtagggcgg
    18961 gagacgatca atgcgcgtaa agcgacgttc agggcgagtg tcagccataa tttcctcaga
    19021 ttaacgttag cgcccggacc gtccgagcga cgctgccaca atggtggctg ttttgaaaat
    19081 agcctgatta atttctgtct gtccagcgtt ttagaacatt tttatcaatg gctttaaatg
    19141 aggaactcca cttcaaaaac aggaaatttt tctacgctaa caagaaaatt accaccaatt
    19201 ttattaacat tataatttaa ataaatttac cagccagaag tgcattatca agagatacat
    19261 tgctaataca ccatttatct ttcaatcccc ttttaaattt atggtttttc ctcaaaaacg
    19321 gcctgctggt catcagcctt caggttacct atcatagagg tttaatcctt attcagagtc
    19381 acccgtgcac gaaatattca acatgctgct ggcggtcttc gatcgggcag cgttaatgct
    19441 tatctgcctg ttctttctca tccgtatccg cctgtttcgc gagctgttgc acaagtcggc
    19501 acactcccca aaagagttgc ttgctgttac cgccattttc tcgctgttcg ccctgttcag
    19561 cacctggtcc ggcgttcccg tagaaggctc gctggtgaac gtacgtatta tcgcggtgat
    19621 gtccggcggg attctgtttg gcccgtgggt aggcatcatt accggcgtga ttgcgggtat
    19681 tcaccggtat ttaattgata tcggcggcgt gacggcgatc ccctgcttta tcaccagcat
    19741 tctggcgggt tgtatatcag gctggataaa cctgaaaatc cccaaagcac agcgctggcg
    19801 cgtcggtatt ctcggcggca tgttgtgtga gacgttgacc atgattctgg taattgtctg
    19861 ggcaccaact accgcgttgg ggatcgatat cgtctctaaa atcggcattc caatgatcct
    19921 cggtagcgtc tgtatcggct ttattgtgct tctggtgcaa agcgttgagg gcgaaaaaga
    19981 ggccagcgcc gcgcggcagg ccaagctggc gctggatatc gccaacaaaa cgctaccgct
    20041 gtttcgccat gtcaatagcg agtcattacg caaggtctgc gaaattatcc gcgatgacat
    20101 tcacgccgat gcggtggcga ttactaatac cgatcatgtg ctggcctatg ttggcgtggg
    20161 tgaacataac tatcagaatg gcgatgactt cattagcccg actacccgtc aggcgatgaa
    20221 ttacggaaaa atcatcatta aaaacaatga tgaagcccac cgcacaccag agattcattc
    20281 catgctggtg atcccattgt gggagaaagg cgttgtgacc ggaacgctga aaatttacta
    20341 ctgccacgcg catcagataa cctcgtcatt acaggaaatg gcggttggtc tgtcgcaaat
    20401 catctccacg caactggagg tttcacgcgc cgagcagcta cgtgaaatgg caaataaggc
    20461 agagcttcgc gccctgcaaa gcaaaattaa tccccatttt ctgtttaacg ctctgaacgc
    20521 tatttcatcg tcaatccgtc tgaatccgga taccgctcgc cagttgatct ttaatctgtc
    20581 gcgttatctg cgctataaca ttgaattaaa agacgatgag caaatcgata tcaaaaaaga
    20641 gctgtatcaa attaaagact atattgccat tgagcaggcc cgctttggtg acaagctgac
    20701 ggttatctat gatattgatg aagaggtgaa ttgctgcatt cccagcctgc tgatccagcc
    20761 gttggtggag aacgccattg tccacggtat tcagccttgc aaaggtaaag gcgttgtcac
    20821 catcagcgtt gcagagtgcg gaaatcgggt acgcattgcg gtgcgagata ccggcacggc
    20881 atcgatccaa aggtgattga gcgggtcgaa gcgaatgaaa tgccgggcaa taaaattggc
    20941 ctgctgaatg tccatcatcg cgtgaagtta ttgtatggcg aggggctgca tatccgccgc
    21001 ctggagccgg ggacggaaat tgcgttttac attcctaacc aacgcacccc agtcgcctca
    21061 caggctacgt tattgctttg agccggagtg atattgtgaa agtcatcatt gttgaagacg
    21121 aattcctggc acaacaggaa ctgagctggc taattaaaga gcacagccag atggagattg
    21181 tcggcacctt tgacgacggt ctggacgtgt tgaagttttt gcagcataac cgcgtcgacg
    21241 ccatttttct ggatatcaat attccgtcgc tggatggcgt gttgctggcg caaaacatca
    21301 gccagttcgc ccataaaccg tttattgtgt tcatcaccgc gtggaaagaa catgcggtag
    21361 aagcgtttga actggaggcg tttgactaca ttctcaaacc gtatcaggag tcacgtatta
    21421 ccgggatgct gcaaaaactg gaagcggcct ggcaacaaca gcagaccagc agtacgcctg
    21481 ccgcgacggt aacgcgtgag aatgacacca ttaatctggt gaaagatgag cgaataatcg
    21541 tcacgccaat taacgatatc tattacgccg aagcgcacga gaaaatgacc tttgtctata
    21601 cgcggcgtga atcctacgta atgccgatga acattaccga attttgcagc aaactgccgc
    21661 cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa tctgaacaaa atacgcgaaa
    21721 tcgaaccgtg gtttaataac acctacattc tgcgactgaa agatctggat tttgaagtgc
    21781 cggtcagccg cagcaaagtg aaagaatttc gccagttaat gcatctttaa tcgggaactt
    21841 tcatgaaagc accaggcttg cccgccgatc aacaattttt cgccgatctg ttcagcggcc
    21901 tggtgcttaa ctcgcaacta ctggggcgcg tctggtttgc cagccagcct gcctcgttac
    21961 cggtgggcag tttatgtatt gattttcccc gtctggatat cgtgctgcgc ggcgaatacg
    22021 gcaacctgct ggaagcaaag cagcagcgaa tggtggaagg agaaatgctg tttattccgg
    22081 cacgcgcggc taatttaccg atcaacaaca aaccggtgat gctgttaagc ctggtgttcg
    22141 caccgacctg gcttgggtta tcgttttacg atagccgcac cacgtcgttg ttgcatcctg
    22201 ctcgccagat ccagcttccc agcctgcaac gcggtgaagg tgaagcgatg cttaccgccc
    22261 tcactcatct tagccgttcg ccgctggagc agaatatcat tcagccactg gtgttaagtt
    22321 tgctgcatct ttgccgcaac gtggtgaata tgccgccggg taattcgcaa ccgcgcggcg
    22381 attttctcta tcacagcatt tgtaactggg ttcaggataa ttatacccag ccgctcaccc
    22441 gcgagagcgt ggcgcagttt tttaatatca cgcccaatca tctgtcaaaa ctgtttgctc
    22501 agcatggaac gatgcgtttt atcgagtatg tgcgttgggt gcgaatggcg aaggcgagga
    22561 tgattttgca gaaatatcat ctgtcaattc atgaagtggc acagcgttgc ggttttccgg
    22621 atagcgacta tttttgtcgc gttttccggc gtcagtttgg tctgacgccg ggagagtaca
    22681 gcgcccgttt tcagggctaa cgtcagaagg ttaattctgt ttccagcagc gtcaggatac
    22741 tttgtgcatc ttgcgcggca aacaacgact ggcggaagtt tttattcacc agtttacgcg
    22801 ccagctgcga gaagactttc acatgattca tcccttcgtt agcacccagc gtcagcatga
    22861 tcaccagttc gacttcgccc atttctgact gccagtcaat cggtttcgcc agccgggcaa
    22921 tgctgatact ggaatgacgg atccactgag atttggtgtg cgggatcgct acgccaaaac
    22981 caacgccggt ggtaacaatc tcttcccgct gccagacatc ttcttccagc tcaaacggat
    23041 gttcagtgcg cccgttaacg ccgaggttgc cgcacaggaa ctggatcgcc tgctctttat
    23101 tgctaaaatc ctgatcaaca aagatattct ccagcgccag cagtgggcga acgtcttctt
    23161 ccggcgtaaa ggcggtgagt aacgcttcaa tttcctgcgc actgcggcat tcacatgcct
    23221 gacgcgccag ttcccgacac gcctcgctat ccagttgacg aagctggctt ttcaccgccg
    23281 gaatacgcgg gctactcata ctcagctcat ccaggcccag cccaagcagt agcggcagat
    23341 aacggctttc accgcccagt tcaccgcaaa tgcctaccca tttgccccgc tgatgcgcag
    23401 tggtaactat ttgctgcaac atgcgcagga acgatggcgt aatcgggtta tatagcggcg
    23461 atacgcgcgg gttattacga tcgaccgcat acagatactg ggtcatatcg ttggagccga
    23521 tactgaagaa atcgacctca tcgcagaagt ggtcgatgat gtagcacacc gacggcactt
    23581 ccaccatgat cccaagcgta atcgtctctg catgacgcag gccatcgcgc ttaagctcaa
    23641 cgatcgcttt ttggatctcg cccttcaccc ataagatctg atcgagactg tggaccatcg
    23701 ggatcatcaa ctgggcgttg ccaaaactgg cggcgcgcaa aatcgcccgt agttgagtgc
    23761 ggaacaggcc agcaaattcc gggtaaatac gtaccgcgcg atagccgagg aacgggttct
    23821 cttcctgggg aatattcaga taaggaatgc ttttatcgcc accgatgtcc attgtgcgga
    23881 agataatcgg cttgtcgccc gctgccagta gcacctgctg gtaggcttca aattgctcct
    23941 gctcgtccgg cgcgctgtcg cgatccatat agagcatttc agtacggaac agtccaacgc
    24001 cttccgcgcc gttggcaaac gcgcctggcg cttccagagc ggtgccgata ttcgccgcaa
    24061 tatcaatacg cttgttatca cgggaatagg ccagctgcgc ggcggcctgc gcctgttgtt
    24121 tttggcgttt atccgccagc gtctgtgcga cctgataata accgctcacc gcgtcattcg
    24181 ggttaatcgc cagcacgccg cactgggcgt caagcaccgc cggttgcccg gcataacggg
    24241 caatcgcatc cagcggcagg ccgctcagta ccgggatcgc cgaggcgcgg gccagaatca
    24301 gggtatgcga ggtgcgccca gttttctcca gaatcatgcc tgcaagattt tttaaatcga
    24361 ggctcaaaaa ctggcttggg gttaaatcct cggctaccag aatagtcggt ttttccagca
    24421 ccagattgtt gcgcggcttc agttccggcc aggtgatatg cagcaactgt tcgctaatat
    24481 cgcgaatgtc gctaacacgt tcgcgcagat aatcactggc agaagcagaa agtttggcgc
    24541 aaacctgctc catattgctg atgatcgccg cccccagccc ttgatgctgt tctgtcatca
    24601 gacgacggat attgcccgcg aattcatcat cctgaatcag cgacaaatgg gcgctaagga
    24661 tagttttgct ttcgccgtca ctctcacgca attgctggtt cagttgctcg gcaagcgttg
    24721 caaggctgtg ctccagccgg gtggaatctt gcgcactggc ggggattgcc cgataactgt
    24781 cgaggctgtc gctttgtaac agggtcagcg tacccacgcc gacgctgctt gccagcacat
    24841 tgccgtacag taaatccggg ttcaggcggc ttaatgaacg cggcagcgga tgcgccgtca
    24901 gttctgcctg cgttggctgg atgctgtcgc tgtcgataaa gcgcacctgg atgtactctt
    24961 ccaacacgcg ctgcgcctgc tcttcatcgc tgccgctaat gttcaggctg caactgtcat
    25021 taaataaagt accggtgcca atcagcgcca gcgagctttt cgcatcggcc tttgcgttct
    25081 ggcgatggtt gataaaggtg atttcgcttt gccactggct gcactgttct ttaagttccc
    25141 acgccggacg agcatgaaga ccgtttggca aaggacagag aaattgaatc gttaacataa
    25201 ccgctccggg ttaatcagga gatcatctga aatccgtcag ttgaacaacc gtctcacgcg
    25261 taagacgttt aataagggca gataaaagct gctgcatctg caaaatgtca cggcaatcgg
    25321 caatcgatgc ggcgcaatgt ccgtggcggg ttgctggccc catcaccact gtaggcacgc
    25381 cggtgccggt taagtgcacc gctccgccgt ccgtgccgcc gttgctgaac atatctgcct
    25441 gtaacggcac gccaatttct gccgccacgg tttcgatcca cgcggtaagt tttggcggcg
    25501 caatcagcga cttgtcgctt aacaccagca tcggcccgtt acaaatctga cgatggttag
    25561 ccgcgccata atcaaagttt ttcgcccagc aggcggtatc aagcacaatg gcgacatccg
    25621 gcgacaccgc gcgggtggca gtttgcccgc cgcgtacccc cacctcttcg ctggaactgg
    25681 ccaccagcca cacttccgca ggcagttcag cgctgtgtag ttcgcgcagt aacgtcacca
    25741 gcaggtagca accgaggcgg tcatcaaagg ctttccccat cacccgctgg tgagggagaa
    25801 cctgaaaagt tgtatcaaag gtgacgcgat cgcctggacg aattcccgcc tgcatcactt
    25861 cgtcatacga gcgcgtacca atgtccacgc gcatggcgct gacgtcattc ccctgccggt
    25921 cgccgtcaag caggcctgga attttgcact cttcacgggt ggtgatgcgc accggctgca
    25981 gctggcgggc agccatgcgc acgttgccaa ccggcagcac atcaattgcc ccttcgcggg
    26041 agatgctgcg caccataaat cccacttcgt ccatatgggc gcagatcatc accttcggcc
    26101 ctgtcgattc attgaggcgg atcagcaccg atcccagacc atcaaatcgc acttcttttt
    26161 gcaggcgatc cgcttcttcc agcaggatct gccgcacttc ctgttccgag gaggcgatcg
    26221 catctgcctc gctcaacgct tttaatagcg ataaatccat tatgcctctc ccgtgagcaa
    26281 cactgttttc ggcatggcgt agagcacttc tgcgccttgc ggggtaacca gcacaacatc
    26341 ttcgatgcgc acgccccctt gccctggcaa ataaatcccc ggctccacgg tcagcaacat
    26401 gcctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat ggacttcaat
    26461 gccgatagcg tgaccagtgt tatgaccgaa atagtcgcca aatccagcct cggtaatgac
    26521 ccggcgcgcg gcgtcgtcaa tctgctggca gcgcacgccg gggcgaattg cagagattgc
    26581 cgcgagctgt gcctgcagga caatctgata gacgtcaaac agcgggtgag attcggcgct
    26641 caccccttcg ccattcacca gcaaggtgcg cgtcatatca gagcagtagc cctgatacag
    26701 cgcaccaaaa tcgagagtga caaactcgcc cgctgcaaca atcttgtcgc tggctttgcc
    26761 gtgcggcagc accccgcgcc agccgctggc gacaatggtg tcaaaagagg ctttttctgc
    26821 gccctgctgg cgcataaacc actccagttc agcggctatc tcgcgctcgc tcatccccgc
    26881 ctgaataaag cggcgaatat gctccgctcc gcgatcggca atcccacagg cgaggcggat
    26941 tatctccacc tcctctggcg ttttgatttg ccgcagcaca tccggcgtgg cgctaaccag
    27001 tttcgcattg agttcagact tccagcggtg cgcggtttcc caactcacct gctggccttc
    27061 aaaaccgagc gtttgcaact gctcatcggc aatgatttga ttgacgatag tggtaagcgt
    27121 gttcgtcgcg tcaagcaaat gcagctggta gccttgcgca cgggcttcca cctcaacgaa
    27181 atagctcgaa tctaccagaa tgtgcgcact ttcacggcta ataaccacat agcctgatcc
    27241 ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag gagagaagca ctgcatccag
    27301 ttgttgctcc ttaagccagt cgcgcagcga agcgagtaat gtcatctgga ttttccttct
    27361 tacaggctat cgattaacaa tttgccttta cggaacatca tcagacgcag gaacaccacc
    27421 atcagcgcag ttatgactgc ccccagcgcg atccccgcca tatagacgcc aaggttagtg
    27481 accagcggcc atgcccagat agcggattcc gggaaccatt gcactgcgcc cagccagaca
    27541 gcggcggttg agccgacaat cgcgccgacc atatacgacg gaatggcggt gatggggctt
    27601 tccagcgcaa acggaatcgc accctcacta atccccataa aggcaaggaa catcgcggtc
    27661 ttaccctgcg gataaagttg agcgttgaac aggcgtttgc cggttaaacg acggtcaata
    27721 atggtcgcca gccccagacc aatcggagga ataacgatag cgatagaacg agcggtgacc
    27781 ggtaatacgt ggtcagtggt aaagctgaag gcaacgaaac ctgcggcttt gttgatcggg
    27841 ccaccaaggt cgattgctgt cgccgcggcg atccccattg cgtacatcag cgcgcccttc
    27901 tcacctgcgg cagtcagcac ggtacggata acgccgttga tccagccgcc aaagggggta
    27961 atgacgtagt acatcgccag catgacaaaa atggcggaaa gaatcggtag caaaaatgtg
    28021 gttttgaacg ctaacaggaa atccggtaac tggattttct ggttcatcca cttcacaagg
    28081 taacccgcga cgatagaaat aatcagcgca ccaatgaagg tggacggcac cggcgaagag
    28141 gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt gcgtcggctg ggtagacatc
    28201 aagccgccga taaaaccagc cgggaacgcc agtttgccac cgatagagtt cgccacaaaa
    28261 gcagcaaaca tcggaatggc aaaaccaaac aacacgccgc cgaaggactg cgacagccag
    28321 gcaaatttca gtaaggagag gtcgaaaccg gagaatttcc cgctattaag agcatccatg
    28381 atgccgatct cagcaggaat tttaagccag ctataagcaa tcagctggct gaaagcgagg
    28441 atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa tccctgacat cacatgctgc
    28501 gggagttcac cccagaagct gcttttagag gcctgcggat tcttaactgc cgctgccgca
    28561 ccggatgcgc caggcacaac ggttgcactg cgttttttaa tggccataac aattatccct
    28621 gtcgaatatt actgttgttc agaggcaatc atctcttcga tttctttgat gatgcccgca
    28681 gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt cgcgtgattc gaaacgctca
    28741 ttatcttccg gggtaactgc cacggagtgg atgatgatgg ttgcttcggc gatatcctgc
    28801 gccgtcaggc gattctggat accgtccgcg ccctgggttt caattttcac ttcataaccg
    28861 gcttctaccg ccgcttcttc caacgcctga gcggccataa aggtgtgagc caggcccatt
    28921 gggcaggcac ataaggcaat cagtttctta ctcatcgtta attcctcatt cagtgatagt
    28981 tgacaagtga aattatgaac gtgcaaaaca aatcgccgtt accagacaaa taatgcattt
    29041 actggagaat taatcccgct gtacggaagt gtgacgcagg tcggccttgt gaggggacac
    29101 aaagaaaaat tgaattaact gtatgattta aacgattatc gggagagtta cctcccgata
    29161 taacaggaag gatttacaga atgtgcccta aggtctggcg taaatgtgca ccggaaccga
    29221 gaagacccgg attgtcatgg acgatgagat acaccggaat atcatggaca tattctttaa
    29281 agcgcccttt atcttcaaat gcagcacgga aaccagaggc tttgaagaac tcaaggaagc
    29341 gcggcacgat accgcccgca ataaacacgc cgccaaatgt cccgagattg agcgccagat
    29401 tgccgccaaa acggcccata atgacgcaaa acagcgacaa cgcgcggcgg caatcggtac
    29461 agctgtcagc cagcgcgcgt tcggtaatat cttttggctt gagattttct ggcaggcggt
    29521 tgtcagcttt cacaattgcg cgatacaaat tcaccagccc agggccagaa agcacgcgct
    29581 ccgccgaaac atgaccaatt tccgcacgca atatttcgag gataatggcc tcttcttcac
    29641 tattcggcgc aaaatcaacg tgaccgcctt cgcccggcaa gcttacccaa cgcttatcga
    29701 catggaccag atgcgcaacc ccaagccctg ttccggcacc gtaaaccgca ataggtttac
    29761 cttctaccgg ttctgcgcca ccaaactgaa tcagatgctc ttttttcagc atcgggatcg
    29821 ccatcgatac agcggtaaaa tcgttaataa tttccagatg gctaaaaccg agattctttt
    29881 tcatttcggc aattgagaac gcccaggtat ggttggtcat tgccacccag tcaccggtaa
    29941 ttgggcaagc gatggcaata cagccgtctt tcacctcgac cttatgttct tcaagataaa
    30001 cgcgaatgac cgcttcgagg ctggggtaat caagccctga ataggtctta gcctgcgaga
    30061 tttcaccact ggcaatatca cacagagcaa gacgtgcgtt ggtgccgccc acatcaccga
    30121 ctaatgcata ctttatcatt cttcaactgc tccgctaaag tcaaaataat tctttctcac
    30181 actgtaaata cctgggggca taacaacaac gccggaaagg caggctccct gtaaatatcg
    30241 atctgggtca cacatttact ttatcgtttc agcaccaatt gcagcgatgc ctttttgcaa
    30301 gctgggcaaa ctaagtatct gaccccgcat aaggaataga acatgctcca tccgcgagcc
    30361 agaaccatgt tgttattatc gctccccgcc gtggcaattg ggattgcgtc cagtcttatt
    30421 ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt tgctctggca acgactgccg
    30481 ggaactctgg ggatagccca ggattcacct ctctggatca tcggtgtatt aacgctaacg
    30541 ggtattgcgg tggggttggt tatccgtttc agccagggtc atgccggacc agaccccgcc
    30601 tgtgaaccgc tgatcggcgc accagttccg ccctctgcgc taccaggact tatcgtagca
    30661 ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg aacatccgat catgaccatc
    30721 aatatcgccc ttgccgtcgc cattggcgct cgtctgttac cgcgcgtcaa ccgaatggag
    30781 tggactattt tagcctctgc cggaaccatc ggcgcgctgt ttggcacacc tgttgcagcg
    30841 gcgttgatat tttcgcaaac cttaaatggc agtagtgaag ttccgctatg ggatcgtctc
    30901 tttgcgccgt taatggcggc agcagctggt gcactaacta ccggattatt tttccatccc
    30961 catttttcac tgcccattgc gcattacgga cagatggaaa tgaccgatat tctcagcggt
    31021 gcaattgtcg cggcgattgc catcgcagca gggatggtcg ccgtatggtg cttaccacgg
    31081 ttgcacgcga tgatgaatca aatgaaaaat ccggtgctcg tgctgggtat tggcggattt
    31141 attctcggta ttctgggggt tattggtgga ccagtttcgc tgtttaaagg gctggatgag
    31201 atgcagcaga tggtggcaaa tcaggctttc agcaccagcg attacttttt gctggcggta
    31261 attaaacttg ccgccctggt cgttgctgcc gccagtggct ttcgcggtgg gcgaatcttc
    31321 ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc atgagcacgt acccgccgta
    31381 ccagcggcaa taaccgtttc ttgcgctatt ctcggcatcg tgctggtggt aacacgcgat
    31441 ggctggttaa gtctttttat ggcggcggtc gttgtaccca ataccacatt gctaccgctg
    31501 ctctgtatcg tcatgcttcc ggcatggctg ttattagcag gtaagccgat gatgatggtc
    31561 aatcgtccga agcaacagcc accccacgat aacgtttagc aaaaaatgct ctcctttatg
    31621 atttaagagt tatggcgatg atacggggct ttacctcccc gtaatattgc gttaacaggc
    31681 cgctgacggc ttcgatcgtg aaggagaata acaatgttca ggtcactgtt tctggcggcc
    31741 gccctgatgg catttacccc gcttgcagca aacgcaggtg aaatcaccct actgccatca
    31801 atcaaattac aaattggcga tcgcgatcat tacggtaatt actgggacgg tggtcactgg
    31861 cgcgaccgtg actactggca tcgcaattat gagtggcgca aaaaccgttg gtggcgtcat
    31921 gataatggct atcatcgtgg ctgggataag cgtaaagcgt atgagcgtag ctatcgtgaa
    31981 ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg gacatggaca ccgccattaa
    32041 acacattcgc caatggagca caatgcctga tgcgacgcta gcgcgtctta tcaggcctac
    32101 aaaccggatc tttaacgcgt agggcggata aggcgtttac gccgcatccg ccaatagtgc
    32161 cagatgcgac gctcattcaa ctacaatccc agcgccgtcc ccaccaacaa ccagatattc
    32221 agcgccacga ccagcactac aatcacccag cctgtctgtt ttacgcgttt gctgttcacc
    32281 agatcgccca tcaacttgct gtcactggtg aaaatcagca gtggaaccag cgccagggcg
    32341 ataccaaaac ttaacagcac ctgactcata accagaatcc gtgtcggatc taatcccatc
    32401 agaatgacaa taaatgacgg cagcatggtg actgtacgac gcacccacag cgggatatgg
    32461 aagcgaatga acccctgcat caccacctgc cctgccagtg tccccactac cgttgaggac
    32521 agtccggcag caaccagact taacccaaag accgttgccg cagcatggct taacagcggt
    32581 tgcagcgtca gataagcctc atcaagatcg gcaacaccag tatgaccaga aaagtggaac
    32641 gccgccgcag ctgtagccat catcgccaga ttgacaaaac cggcaatagt catggcgata
    32701 gccacatccc atttggtggc ggaataacgt tgttgacgcg aaccgccatg taaatgctga
    32761 gtgagcgagg agtgcaaata aatcacatgc ggcataatcg tcgcccctaa cacgcctgct
    32821 gccaggaaga ccgcttccga agtaggtaaa ctcgggagca ccattccttt acccagctgc
    32881 gccaggttag gctgggagaa aatcaactcg acaatgtaag ccgcggcaac aaacaacagt
    32941 aacccgccaa tcactttctc cagcgttttt tgcccgcgac gttgcagcat taaaatcagg
    33001 aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg aaacaccaag aatgagttta
    33061 aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat aatttctgcc
    33121 tgaacccaat agaaccacac tacgggacgc ggatagtgat cgcgaatctg ctccgccaga
    33181 tttttaccgg tggcaatccc tagtttggca gagaggatct gaatcagcat cgccatcagg
    33241 ttggcccaaa cgacaaccca cagtagctga tagccgaagc tggcacccgc ctgaatattg
    33301 gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg tcccattaat
    33361 gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct caacgcgata gttcgtcatc
    33421 ttgtgcctct aaaacatagc ctttgctatg tttcatgcta tgataaacga gaatgattat
    33481 caaattcatt taaatggatt gtggtgattt ctgtgataga ccaggattat gactacgaaa
    33541 agattgatga cttgaatgtt atggttatgt ttaatgttag cacatttaca taactttcag
    33601 cttccataca caacatagca gaaatgtatg acagatcact atttttgaag cctgtcacag
    33661 gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat agaatgtgca
    33721 cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc attttgtact
    33781 ggcacttgcc gttgttgcga ttctcgcact gctggtaagc agcgaccgca aaaaaattcg
    33841 tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt tcttcctgaa
    33901 ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa atgttcgaaa aactgctcgg
    33961 atttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag gcctggcatt
    34021 cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg gtattctcca
    34081 gcacattcgc gtgttgccgg tgatcatccg cgcaattggt ttcctgctct ccaaagtcaa
    34141 cggcatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg gtcagtctga
    34201 aaactttatt gcctataaag atatcctcgg caaaatctcc cgtaatcgta tgtacaccat
    34261 ggctgccacg gcaatgtcca ccgtgtcgat gtccatcgtt ggtgcataca tgaccatgct
    34321 ggaaccgaaa tacgtcgttg ctgcgctggt attgaacatg ttcagcacct ttatcgtgct
    34381 gtcgctgatc aacccttacc gtgttgatgc cagtgaagaa aacattcaga tgtccaacct
    34441 gcacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag gtttcaaagt
    34501 tgccattatc gttgccgcga tgctgatcgg ctttatcgcc ctgatcgctg cgctgaacgc
    34561 actgtttgcc accgtgactg gctggtttgg ctacagcatc tccttccagg gcattctggg
    34621 ctacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag cactgcaagt
    34681 gggcagtatc atggcgacca aactggtttc caacgagttc gttgcgatga tggatctgca
    34741 gaaaattgct tccacgctct ctccgcgtgc tgaaggcatc atctctgtgt tcctggtttc
    34801 cttcgctaac ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc tgaatgaaga
    34861 gcaaggtaac gtggtttctc gcttcggtct gaaactggtt tacggctcta ccctggtgag
    34921 tgtgctgtct gcgtcaatcg cagcactggt gctgtaagac catacataaa aaagccgggg
    34981 ataattccc
//
LOCUS       DAJVOY010000022        34978 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVOY010000022 DAJVOY010000000
VERSION     DAJVOY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.022"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..47)
                     /locus_tag="OQL90_000951"
     CDS             complement(<1..47)
                     /locus_tag="OQL90_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1367810.1"
                     /translation="MMKLLIIVVLLVISFP"
     gene            complement(319..375)
                     /locus_tag="OQL90_000952"
     CDS             complement(319..375)
                     /locus_tag="OQL90_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1367811.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            654..1901
                     /gene="mdtA"
                     /locus_tag="OQL90_000953"
     CDS             654..1901
                     /gene="mdtA"
                     /locus_tag="OQL90_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS1367812.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGNRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            1901..5023
                     /gene="mdtB"
                     /locus_tag="OQL90_000954"
     CDS             1901..5023
                     /gene="mdtB"
                     /locus_tag="OQL90_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCS1367813.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLATLDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            5024..8101
                     /gene="mdtC"
                     /locus_tag="OQL90_000955"
     CDS             5024..8101
                     /gene="mdtC"
                     /locus_tag="OQL90_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1367814.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRAPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            8102..9518
                     /locus_tag="OQL90_000956"
                     /pseudo
     CDS             8102..9518
                     /locus_tag="OQL90_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9515..10918
                     /gene="baeS"
                     /locus_tag="OQL90_000957"
     CDS             9515..10918
                     /gene="baeS"
                     /locus_tag="OQL90_000957"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1367815.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            10915..11637
                     /gene="baeR"
                     /locus_tag="OQL90_000958"
     CDS             10915..11637
                     /gene="baeR"
                     /locus_tag="OQL90_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1367816.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            11828..12160
                     /locus_tag="OQL90_000959"
     CDS             11828..12160
                     /locus_tag="OQL90_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS1367817.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            12283..13683
                     /locus_tag="OQL90_000960"
     CDS             12283..13683
                     /locus_tag="OQL90_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCS1367818.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            14004..15365
                     /gene="yegQ"
                     /locus_tag="OQL90_000961"
     CDS             14004..15365
                     /gene="yegQ"
                     /locus_tag="OQL90_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1367819.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(15695..16012)
                     /locus_tag="OQL90_000962"
     CDS             complement(15695..16012)
                     /locus_tag="OQL90_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367820.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            16426..17324
                     /gene="yegS"
                     /locus_tag="OQL90_000963"
                     /pseudo
     CDS             16426..17324
                     /gene="yegS"
                     /locus_tag="OQL90_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            complement(17406..18179)
                     /locus_tag="OQL90_000964"
     CDS             complement(17406..18179)
                     /locus_tag="OQL90_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1367821.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(18285..19325)
                     /gene="gatD"
                     /locus_tag="OQL90_000965"
     CDS             complement(18285..19325)
                     /gene="gatD"
                     /locus_tag="OQL90_000965"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1367822.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITIGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(19373..20728)
                     /locus_tag="OQL90_000966"
     CDS             complement(19373..20728)
                     /locus_tag="OQL90_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1367823.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNIDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            complement(20732..21016)
                     /gene="gatB"
                     /locus_tag="OQL90_000967"
     CDS             complement(20732..21016)
                     /gene="gatB"
                     /locus_tag="OQL90_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1367824.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(21047..21499)
                     /gene="gatA"
                     /locus_tag="OQL90_000968"
     CDS             complement(21047..21499)
                     /gene="gatA"
                     /locus_tag="OQL90_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1367825.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(21509..22771)
                     /gene="gatZ"
                     /locus_tag="OQL90_000969"
     CDS             complement(21509..22771)
                     /gene="gatZ"
                     /locus_tag="OQL90_000969"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCS1367826.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(22800..23654)
                     /gene="gatY"
                     /locus_tag="OQL90_000970"
     CDS             complement(22800..23654)
                     /gene="gatY"
                     /locus_tag="OQL90_000970"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1367827.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(23962..25014)
                     /gene="fbaB"
                     /locus_tag="OQL90_000971"
     CDS             complement(23962..25014)
                     /gene="fbaB"
                     /locus_tag="OQL90_000971"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1367828.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            25271..26548
                     /locus_tag="OQL90_000972"
     CDS             25271..26548
                     /locus_tag="OQL90_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1367829.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPGTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFCESDNEITAIKVDDRDIALTQGEVK"
     gene            26545..27549
                     /locus_tag="OQL90_000973"
     CDS             26545..27549
                     /locus_tag="OQL90_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCS1367830.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSITRHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            27546..28511
                     /locus_tag="OQL90_000974"
     CDS             27546..28511
                     /locus_tag="OQL90_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1367831.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(28485..29231)
                     /locus_tag="OQL90_000975"
     CDS             complement(28485..29231)
                     /locus_tag="OQL90_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1367832.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(29283..30101)
                     /locus_tag="OQL90_000976"
     CDS             complement(29283..30101)
                     /locus_tag="OQL90_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1367833.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQKVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(30166..30966)
                     /gene="thiD"
                     /locus_tag="OQL90_000977"
     CDS             complement(30166..30966)
                     /gene="thiD"
                     /locus_tag="OQL90_000977"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1367834.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIESDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQYV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(30963..31751)
                     /gene="thiM"
                     /locus_tag="OQL90_000978"
     CDS             complement(30963..31751)
                     /gene="thiM"
                     /locus_tag="OQL90_000978"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1367835.1"
                     /translation="MQVDLQSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLPHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(31786..31882)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(33463..33756)
                     /locus_tag="OQL90_000979"
     CDS             complement(33463..33756)
                     /locus_tag="OQL90_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367836.1"
                     /translation="MYGQDTTAGGNLKKDSPLGKLWDFLHSPDLPTNPDFAKPADKTG
                     WVSSYSNPTYVPPTPTITAPQLKVDMAGNPLMTPPAWLNQPLNVNVNVKVQDG"
     gene            complement(33720..>34978)
                     /locus_tag="OQL90_000980"
     CDS             complement(33720..>34978)
                     /locus_tag="OQL90_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005135759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS1367837.1"
                     /translation="MIEGAGGAITIAATVALSGKIMTTGQDFESALSGLTAITGSTDK
                     AAKEFDYLRDQSNRLGLDLLKTSRDYTQFAAAVGDKLPKDQMRSIFEGASEWGLVTGA
                     SADEASRALKSLNQMMSKGTVMSEELKGQLSEALPGAVGLFVKALNQMKGTTNLTEKD
                     LFDLMQQGKLFSKDILPEVAKQMKAAARNGGALDKAMKSNRASWQRLQTSMQNAMNIF
                     FASGFGNELTSAFDAISAAVNGSGGAFEMFGHIAGKIVEGATDAFTELHDTVILTFRI
                     IEYYASKMGITGEQLKAWGEMAAYAAGVAMFAGSIWKLGVAFKWLLTMLNPLTKLLGV
                     MKGIAAIGGIEAATTGGSGGEGKDKPNKPGRGPKVGKVGGAAALSGGPVGWSIAVGSV
                     GGDYAWDKVMTLTTPCMDRTPPPGAI"
BASE COUNT         8357 a         9157 c         8790 g         8674 t
ORIGIN      
        1 gggaagctta tgactaagag caccacgatg atgagtagct tcatcatgac cctttcctta
       61 tttaaggccc cttcctcggg aggggctttc ccgtttcagc gtcccgctga aatcgtcggc
      121 ttacctcctt tcgccctgta tgcagtctat cgctgacttg cggataaaat agtttccagt
      181 gtgattattg cgtaacgtct cgcaaagaat atttgctggc aggatcgcag actgcaaagc
      241 ctgcagattg acaatcttat cgtgaaggca tactttcagg agtgagggta gagcggggtt
      301 tcccccgccc tggtagtctt agtaagctgg ccagctaatg actaagagca ccacgatgat
      361 gagtagcttc atcatgaccc tttccttatt tatggcccct tcctcgggag gggctttccc
      421 gtttcagcgt cccgctgaaa tcggcggctt acctcctttc gccatgcaag cagtctatcc
      481 ctaacgcgta gataaaatag tttcctgtgt tattactgga tgcgtgctcg caaatgtgcc
      541 cgtcattcag acaattcccg acagtgtttc ataattcctc catttttctc ccttattggc
      601 tggctacact agtatcattc cgcgaaacgt ttcaggaaga gaaactctta acgatgaaag
      661 gcagttataa atcccgttgg gtaatcgtaa tcgtggtggt tatcgccgcc atcgccgcat
      721 tctggttctg gcaaggccgc aatgactccc ggagtgcagc cccaggggcg acgaaacaag
      781 cgcagcaatc gccagcgggt ggtcgccgtg gtatgcgttc cggcccatta gccccggttc
      841 aggcggcgac cgccgtagaa caggcagttc cgcgttacct caccgggctt ggcaccatta
      901 tcgccgctaa tactgttacg gtgcgcagcc gcgtggacgg tcaactgatg gcgttacatt
      961 tccaggaagg ccagcaggtc aaagcaggcg atttactggc agaaattgac cccagccagt
     1021 tcaaagttgc attagcacaa acccagggcc aactggcaaa agataaagcc acgcttgcta
     1081 acgcccgccg tgatctggcg cgttatcagc aactggcaaa aaccaatctc gtctcccgtc
     1141 aggagctgga tgcccaacag gcgctggtca gtgaaaccga aggcaccatt aaggctgatg
     1201 aagcaagcgt cgccagcgcg cagttgcaac tcgactggag ccgcatcacc gcaccagtcg
     1261 atggtcgcgt tggtctcaag caggttgatg ttggtaacca aatctccagt ggtgatacca
     1321 ccggaattgt ggtgatcacc cagacgcatc ctatcgattt agtctttacc ctgccggaaa
     1381 gcgatatcgc tactgtagtg caggcgcaaa aagccggaaa accgctggtg gtagaagcct
     1441 gggatcgcac caattcgaag aaattaagtg aaggcacgct gttaagcctc gataaccaaa
     1501 tcgatgccac aaccggtatg attaaagtga aagcacgctt taataatcag aatgatgtgc
     1561 tgtttcccaa tcagtttgtt aacgcgcgca tgttagtcga caccgaacaa aacgccgtag
     1621 tgatcccaac agccgccctg caaatgggca atgaaggcca ttttgtctgg gtgctgaata
     1681 gcgaaaacaa ggtcagcaaa catctggtga cgccgggcat tcaggacagt cagaaagtgg
     1741 tgatccgtgc aggtatttct gcgggcaatc gcgtggtgac agacggcatt gatcgcctga
     1801 ccgaaggggc gaaagtggaa gtggtggaag cccagagcgc caccactccg gaagagaaag
     1861 ccaccagccg cgaatacgcg aaaaaaggag cacgctcctg atgcaggtgt tacccccgag
     1921 cagcacaggc ggcccgtcgc gcctgtttat tatgcgtcct gtcgccacca cgctgctgat
     1981 ggtggcgatc ttactcgccg ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc
     2041 ggaagtggac tatccgacca ttcaggtggt cacgctctac ccaggtgcca gcccggatgt
     2101 catgacctct gccgttaccg cgccgctcga acgccagttc gggcagatgt ctggcctgaa
     2161 acagatgtcg tcgcaaagtt ccggcggtgc gtcagttatc actttgcagt tccagctaac
     2221 attaccgctc gatgtcgccg agcaggaagt gcaggccgcg attaacgctg cgaccaactt
     2281 gttgccgagc gatctgccta acccgccggt ttacagcaaa gtgaacccgg cagatccgcc
     2341 gatcatgacg ctcgccgtca cctcaaccgc catgccgatg acgcaagtgg aagatatggt
     2401 ggaaacccgc gtcgcgcaga aaatctcgca gatttccggc gtcggcctgg tgacgctttc
     2461 cggcggtcag cgtccggctg ttcgcgtcaa acttaacgct caggcgattg ccgccctcgg
     2521 cctgaccagc gaaaccgtgc gcaccgccat taccggcgct aacgttaact cggcaaaagg
     2581 tagcctcgac ggcccttccc gtgcggtcac gctttccgcg aacgaccaga tgcaatccgc
     2641 cgaagagtat cgccagctaa tcatcgccta ccagaacggc gcgccaattc gtctgggcga
     2701 tgtcgcaact gtagagcaag gtgcagaaaa cagctggctc ggcgcgtggg cgaacaaaga
     2761 acaggccatt gtgatgaatg ttcagcgcca gcccggtgct aacattatct ccaccgccga
     2821 cagcattcgg cagatgctgc cacagctcac tgagagtctg ccgaaatcgg tgaaggtgac
     2881 agtgctttcc gatcgcacca ccaatatccg cgcatccgtc gatgatactc agtttgaatt
     2941 gatgatggct atcgcgctgg tagtcatgat tatctacctg tttttgcgca atattccggc
     3001 gaccatcatt cccggtgttg ctgtaccgct gtcgttaatc ggcactttcg cggttatggt
     3061 gtttctcgat ttttcaatca ataacctgac actgatggcg ttaactatcg ccaccggatt
     3121 cgtggtcgat gacgccatcg tggtgatcga aaacatttcc cgctatatcg aaaaaggcga
     3181 aaaaccgttg gcggcggcgc tcaagggcgc aggtgaaatc ggctttacca ttatctcgct
     3241 gatcttctca ctgattgcgg tgttgatccc actgctgttt atgggcgata tcgtcgggcg
     3301 actgttccgc gaatttgcta ttaccctggc ggtagcgatt ttgatctcag cggtggtgtc
     3361 gctgaccctg acaccgatga tgtgcgcgcg gatgctcagc caggagtcgt tgcgtaaaca
     3421 gaaccgcttc tcccgtgcct cggaaaaaat gttcgacagg ataatcgccg cctatggtcg
     3481 tggactggcg aaagtgctga atcatccgtg gctgacctta agcgtggcac tcagcacgct
     3541 gctgcttagc gtgctgctgt gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa
     3601 tggcattatt cagggcactt tgcaggcacc gcaatccagc tcctttgcca atatggccca
     3661 gcgacaacgc caggtcgcgg acgtgatttt gcaggatccg gcagtgcaaa gcctgacctc
     3721 atttgttggc gttgatggca ctaacccgtc gctgaatagt gcacgtttac aaatcaacct
     3781 caaaccgttg gatgaacgtg atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc
     3841 ggtagataaa gtgccgggcg tcgatctctt cctgcaacca acgcaggatc tgactattga
     3901 tactcaggtc agccgcaccc agtaccagtt taccttgcag gccacctcac tggatgcgct
     3961 cagtacctgg gtgccacagt tgatggaaaa actccagcaa ctgccacagc tttctgatgt
     4021 ctccagcgac tggcaggaca aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc
     4081 cagccgtctg gggatcagca tggcggatgt cgataatgcc ctgtacagcg cgtttggtca
     4141 gcgactgatt tccactattt atactcaggc taaccagtat cgcgtagtgc tggagcacaa
     4201 caccgaaatt accccgggcc tcgcgacgct ggataccatt cgcctgacca gtagcgacgg
     4261 cggcgtggtg ccgttaagct caattgccaa agttgaacag cgttttgcgc cgctttccat
     4321 caaccatctg gatcagttcc cggtaacgac catctccttt aacgtgccgg ataactattc
     4381 gctgggcgat gcggtgcagg cgattatgga caccgaaaag acgctgaatc tgccggtgga
     4441 tatcaccacg caattccagg gcagcaccct cgccttccag tcggcgctgg gcagcactgt
     4501 ctggctgatt gtcgcggcgg tggtggcgat gtatatcgtg ctcggcattc tgtacgagag
     4561 ctttattcac ccgatcacca ttctctcgac gctacccacc gcaggggtcg gcgcgctgct
     4621 ggcattaatg attgccggta gcgaactgga tgtgattgcg attatcggca ttattttgct
     4681 gatcggtatt gtgaagaaga acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg
     4741 cgagcaaggc atgtcgccgc gcgatgcaat ctaccaggct tgtctgttgc gttttcgtcc
     4801 gatcctgatg accaccctgg cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg
     4861 ggtcggcgcg gaactgcgtc gtccgttagg tatcggcatg gtcggcggtc tgattgtcag
     4921 ccaggtgctg acgctgttca ccacgccggt gatttatctg ctgttcgacc gcctggcatt
     4981 gtggaccaaa agtcgctttg ctcgccatga agaggaagcg taagtgaagt tttttgccct
     5041 cttcatttac cgtccggtgg cgacgatttt actgtcggtt gccattaccc tgtgcggcat
     5101 actgggcttc cgtatgctgc cggtcgcccc gctgccgcag gtcgattttc cggtgattat
     5161 ggtcagcgcc tcgctgcccg gtgcgtcacc agaaacaatg gcgtcttccg ttgccacgcc
     5221 gctggagcgc tcacttgggc gcattgccgg agtcagtgaa atgacctcca gcagttcgct
     5281 cggcagcacg cgtattattt tgcagtttga ttttgaccgg gatatcaacg gcgcggcgcg
     5341 cgatgtgcag gcggcgatca acgctgcaca aagtttgctg cccagcggga tgcccagccg
     5401 tccgacctat cgcaaagcta acccgtcgga tgcgccaatt atgatcctca cgctgacatc
     5461 cgatacttat tcgcagggta aactgtacga tttcgcctcg acgcagctgg ctccgacgat
     5521 ttcgcaaatc gacggtgttg gtgatgtcga tgtcggcggc agctcactgc ccgccgtacg
     5581 cgtcgggctg aatccgcagg cgctgtttaa tcagggcgtc tcgctggacg acgtacgcac
     5641 tgccatcagc aatgccaacg tgcgtaaacc gcagggggcg ctggaagatg gcactcaccg
     5701 ctggcagatc cagaccaatg atgagctaaa aaccgccgcc gaatatcagc cgctgattat
     5761 tcattacaac aacggcggcg cggttcgtct gggcgatgtg gcgacagtga ccgactctgt
     5821 acaggatgta cgtaacgccg ggatgaccaa tgccaaaccg gctattttgc tgatgatccg
     5881 caagctgccg gaagccaata ttatccagac ggttgacagc atccgggcaa aattaccgga
     5941 gttgcaggaa accattccgg cggcgattga tctgcaaatt gctcaggatc gcgcccccac
     6001 cattcgcgcc tcgctggaag aagtcgagca aacgctgatt atctcggtgg cgctggtgat
     6061 tctggtggtg tttttattcc tgcgctcggg tcgcgcaact attattcccg ccgttgcggt
     6121 gccggtttcg ctgattggta cgtttgcggc gatgtacctg tgcggtttca gtctcaataa
     6181 tctttcgtta atggcgctca ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt
     6241 gctggaaaac attgcccgtc atctggaagc gggaatgaaa ccgttgcaag ccgcactgca
     6301 aggtactcgc gaagtcggtt ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt
     6361 cctgccgctg ctgttgatgg gcggattgcc gggccgactg ttacgcgaat ttgccgtgac
     6421 gctttctgtc gccattggta tatcgttgct ggtttctctg acattaacgc caatgatgtg
     6481 tggctggatg ctgaaagcca gcaagccgcg cgagcaaaag cgactgcgtg gttttggtcg
     6541 catgttggta gccctgcaac aaggctacgg caagtcactg aaatgggtgc tcaatcatac
     6601 ccgtctggtg ggcgtggtgc tgcttggcac cattgcgctg aatatctggc tgtatatctc
     6661 gatcccgaaa accttcttcc cggagcagga cactggcgtg ttgatgggcg ggattcaggc
     6721 ggatcagagt atttcgtttc aggcgatgcg cggtaagttg caggatttca tgaaaattat
     6781 ccgtgacgat ccggcagtgg ataatgtcac cggctttaca ggcggttcgc gagtgaacag
     6841 cgggatgatg tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat
     6901 tatcgaccgt ctgcgcgtaa aactggcgaa agaaccgggg gcgaatctgt tcctgatggc
     6961 ggtacaggat attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt
     7021 atccgacgac ctggcggcac tgcgagaatg ggagccgaaa atccgcaaaa aactggcgac
     7081 gttgccggaa ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct
     7141 ggtttacgac cgcgacacca tggcacggct gggaatcgac gtacaagccg ccaacagtct
     7201 gttaaataac gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta
     7261 taaagtggtg atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat
     7321 gttcgttatc aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc
     7381 ggcgaatgcc ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatttcgtt
     7441 taacctgccg accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac
     7501 ccagcttggg gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggtgttcca
     7561 ggagacgatg aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt
     7621 gctgggtatc ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc
     7681 ggcgggcgtt ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc
     7741 cctgataggg atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga
     7801 ttttgcgctt gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc
     7861 ctgtctgctg cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggtgcgct
     7921 gccgctggta ttgtcgggcg gcgacggctc ggagctgcgg caacccctgg ggatcaccat
     7981 tgtcggcgga ctggtaatga gccagctcct tacgctgtat accacgccgg tggtgtatct
     8041 ctttttcgac cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta
     8101 aatgacagat cttcccgaca gcacccgttg gcaattgtgg attgtggctt tcggcttctt
     8161 tatgcagtcg ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct
     8221 cggggaaagt ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt
     8281 gatgctgccc gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac
     8341 cgccatcgtg ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga
     8401 actgttgctg gcacgcgcgt tacagggcgt tggcggcgcg atgatggtgc cggtcggcag
     8461 attgacggtg atgaaaatcg taccgcgcga gcaatatatg gctgcgatga cctttgtcac
     8521 gttacccggt caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtggagta
     8581 cgcgtcgtgg cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc
     8641 cacattgctg ttaatgccga actacaccat gcagacgtgg cgctttgatc tctccggatt
     8701 tttattgctg gcggttggca tggcggtatt aacccctggc gctggacggc agtaaaggta
     8761 caggtttatc gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc
     8821 tttatctgct gcacgccaga aataacaacc gtgccctgtt cagtctgaaa ctgttccgta
     8881 ctcgtacctt ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt
     8941 tgccctttat gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg
     9001 gactgatgat gatcccgatg gtgcttggca gcatgggaat gaagcgaatt gtggtacagg
     9061 tggtgaatcg ctttggttat cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg
     9121 tcaccctgtt gtttatgact accgccctgc tgggctggta ctacgttttg ccgttcgtcc
     9181 tgtttttaca agggatggtc aactcgacgc gtttctcctc catgaacacc ctgacgctga
     9241 aagatctccc ggacaatctg gcgagcagcg gcaacagcct gctgtcgatg attatgcaat
     9301 tgtcgatgag tatcggcgtc actatcgccg ggctgttgct gggacttttt ggttcacagc
     9361 atgtcagcgt cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg
     9421 cgttgatcat cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa
     9481 atgtagctat ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac
     9541 cggcaaactg tttctggcga ttttcgccac ctgcattgtc ttgctgatca gtatgcactg
     9601 ggcggtgcgt atcagttttg agcgtggctt tattgattac atcaagcatg gtaatgaaca
     9661 gcgattacaa ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg gcaactggcg
     9721 cttcctgcgc aacaatgatc gctttgtctt tcagatcctg cgttcatttg aacacgataa
     9781 ttcggaagat aaacccggcc cgggtatgcc accgcacggc tggcgtaccc agttctgggt
     9841 ggttgatcaa aacaacaaag tgctggttgg tccgcgagcg ccgattccac ctgacggtac
     9901 acggcgaccc attctggtca acggtgcgga agttggcgcg gtgatcgcct cccccgttga
     9961 gcggttaacg cgcaatactg atatcaattt cgataaacaa cagcggcaaa ccagctggtt
    10021 gattgtcgcc ctggcaacgt tactcgcggc acttgccact tttctgctgg cgcgcggttt
    10081 actggcaccg gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac
    10141 tacccgcgta acgcccacca gtgaagatga actgggcaaa ctggcgcaag acttcaacca
    10201 gcttgccagc acactggaga aaaaccagca aatgcggcgc gattttatgg ccgatatttc
    10261 tcacgaactg cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg
    10321 cgtgcgtaaa ttcacgccgg agacggtggc gtctttacag gcggaggtcg gtacactgac
    10381 caaactggtt gacgatctcc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca
    10441 aaaagcaccg gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg
    10501 attcgccagt cgtggcctga aactgcaatt ttccctgcca gacagtatta ccgtatttgg
    10561 cgatcgcgac cgtttaatgc agttattcaa taatttactt gaaaacagcc tgcgctatac
    10621 tgacagcggc ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac
    10681 ctttgccgac agtgcgccag gtgtcagtga cgagcagcta caaaaattat ttgaacgttt
    10741 ttatcgcacc gaaggttccc gcaaccgcgc cagcggtggt tccgggctgg ggctggcgat
    10801 ttgcctgaac attgttgaag cacataatgg ccgcattatt gctgcccatt cgccttttgg
    10861 cggggtaagc attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc
    10921 gagttaccaa tcgacgaaaa cacaccacgt attttgatcg tggaagatga accgaagctg
    10981 gggcagttgc tcattgatta tctgcgtgct gcgagctatg cgccgacgct tatcagccac
    11041 ggcgatcagg tactgccgta tgtgcgccag acaccaccgg atctgatcct gttggatctg
    11101 atgctccctg gcaccgatgg cttgacgctg tgccgggaaa ttcgtcgttt ttctgacatt
    11161 cctatcgtga tggtgacggc aaaaatcgaa gagatcgacc gcctgctcgg actggagatt
    11221 ggcgcagatg actacatctg caagccgtac agcccacggg aagtggtagc gcgcgtcaaa
    11281 accattttgc gccgttgcaa accgcagcgc gaattgcagc aacaggatgc cgaaagcccg
    11341 ttgattatcg acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgaca
    11401 cctgcggaat ttcgtctgtt gaaaacgctc tctcatgaac caggaaaagt gttctcccgc
    11461 gagcaattgc tcaatcatct ttatgacgac taccgcgtag taaccgaccg caccattgac
    11521 agccacatta aaaacctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc
    11581 cgcgccgttt acggcgttgg ttaccgctgg gaagccgacg cctgccgcat cgtttagttt
    11641 tagcgacatg actttgttag ccggagatgg cgttcagacc agatccggca acattatccc
    11701 acgcatggtc agcaaactga cattatctct ccgccctggc aggcatcaga aaatcgctca
    11761 tactttaatc ggtaaacagc acctttagat gctgttttcg atacgcaatt tcaatcaagg
    11821 agtcattatg gctggttggt ttgaactcag taagagcagt gataatcagt tccggtttgt
    11881 gctaaaagcg ggcaatggtg agactatcct caccagcgag ctgtatacat caaaagcctc
    11941 tgcggaaaag ggcatcgcgt cagtgcgtag caacagcccg caagaagaac gctatgagaa
    12001 aaaaacggca agtaacggca aattctattt caatctgaaa gccgccaatc atcaaattat
    12061 cggctccagc cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa
    12121 agccaatggc acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt
    12181 tgttggcgtc cggctttatt ttctctcatc acagagttac tatccctcaa gaaattatta
    12241 accatgaaat gagttttctc ctcataactg ataaggaaaa ttatgctcaa aggattgcgt
    12301 acattatttt cacgcagggt tttgtttaac gtcgaaatga ataattctgt tgactgtgaa
    12361 ttacttctca tccagcaaca actccaggca ttgatagccg aaccaggcaa ttatttagca
    12421 catgattgtg atctcaacga tactctggtt ctttgccagc ttacccatca tcacaatgga
    12481 tatatacgag aaaaggccgt gcagtatctg gggcacaaag aggatgttgc ggctatagaa
    12541 gaattattac ttgcagctaa tgactgggta aaagaggttc gccttgctgc aaccatagca
    12601 ttacgtcatt taatgaagga taagaatgca gcggcatttg ccgttaatct acccatgata
    12661 cgtaaccttc tccaatgcca gcgttacgat caccgtgcat ttgtagacga gatacttaga
    12721 tatttattga cagaaaataa ccgcccacaa ttaacgtatt ggcttacttg ccctgataaa
    12781 aagctctccc gcgcggtatt acaaacgctg attgaatacg gttactttaa tgatgaatct
    12841 tcactgcttt caatactcga taaacccgat gagggtttgc gaatgctggc gaccacccac
    12901 tggctgcgtc aacaattacc attaagcgaa gcggtattaa ctcgtctgtt aaaagaccgc
    12961 tggccgcgta ttcgacaagc aacgttattt agcctcactg accgagcaat agaaatgcct
    13021 ccggcgttgc acagcacatt actgctggat aacaatatgt tgatccgcct acgcgctaaa
    13081 aatatgcttc acgaagtcat ggatgttccg caattctggc gtcatgttgt cacttctgcg
    13141 gaatatactc cctcacagcg acgggccgct ttatacgggc tggattccat tcatgatccc
    13201 aatattttaa aactggcaga atggggatta tcacagaatg tattcccact gcgtttagcc
    13261 gctatgcaca ttcttgcaaa agctaatcct cgttgtggcg tcaaagagac tattctgacc
    13321 acgctggcta acccggacgc cgccgggttg cgatttatgg ttaacatctg tgtatggtgt
    13381 cgcgttccgc tcacgttcga ggaaatacgt cagttgcagg aaaacgcgcc gtcagttaaa
    13441 catgcttgcg catattgtcg gctgtatcat aacttgaaca aatgggacgg tttaattctt
    13501 cttcttcaat cgcaacacaa gctaactgaa gaatttgcag gtaaacagct cgcgatatgg
    13561 caacgcaatt tcaatctttc gggcattcaa ccgaatgctc ttcagcgtca gcaacttcag
    13621 gcgcttttta cccgcaatcc ggaacttcac aacagattat ggggttacat tccttttaaa
    13681 taacttcgaa ttatagactt acgatgacaa atgtacctgc tgacgagcgg aaccaaaacg
    13741 aaacaatacg tgaagcttta cgcttgctac gagaaaaact tgccgaggga ctacgcagtg
    13801 gcgaagccca accgtgggaa aaggatctgt ttttacataa agtgaaggcc ggtaacaaat
    13861 aaatccccca gtcttttccc tcacatttgc actggagcca accccgccga ttgcgctaca
    13921 atgcccgccc ttaaagtggg ggcactcccc taaccgcttc atcaggtgaa gcggatctga
    13981 cctgtcatca gaacgagaga attatgttta aaccggaact cctttccccg gcgggaacgc
    14041 tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg ggccagccgc
    14101 gttactccct gcgtgtgcgc aacaacgaat tcaaccacga aaatcttcag ctcggcatca
    14161 atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt caacattgca ccgcacaacg
    14221 ccaagctgaa aacctttatc cgtgacctga aaccggtggt ggaaatgggg ccggatgcgc
    14281 tgattatgtc cgatccaggg ctgattatgc tggtgcgtga gtacttccct gaaatgccga
    14341 tccacctctc ggtgcaggct aacgccgtaa actgggcgac ggtgaaattc tggcagcaaa
    14401 tgggcctgac ccgcgtgatc ctctctcgcg aactgtcgct ggaagagatt gaagagatcc
    14461 gcaatcaggt gccggatatg gagatcgaga tcttcgttca cggtgcgctg tgcatggcct
    14521 actccggtcg ctgcctgctc tctggctata tcaacaagcg cgatccgaac cagggtacct
    14581 gcaccaacgc ctgccgctgg gagtacaacg tccaggaagg gaaagaagat gacgttggca
    14641 acatcgtaca caagtacgag ccgatcccgg tgcaaaatgt tgagccgacg ctaggtatcg
    14701 gcgcgccaac cgacaaagtg tttatgatcg aagaagccca gcgtccgggc gagtatatga
    14761 ccgcgtttga agatgagcac ggcacttaca tcatgaactc gaaagatctg cgcgccatcg
    14821 cccatgtaga acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa ggccgtacta
    14881 aatctttcta ctattgcgca cgcaccgcac aggtttatcg taaagctatc gatgatgccg
    14941 ctgcgggcaa accgttcgat accagcctgc tggaaactct ggaaggtctg gcgcatcgtg
    15001 gctataccga aggtttcctg cgtcgtcata ctcacgacga ttatcagaac tacgaatacg
    15061 gttattcagt ttctgaccgc cagcagtttg ttggtgagtt taccggtgag cgcaaggggg
    15121 acctcgcagc ggtagcggtg aaaaataaat tctccgttgg cgacagcctt gagctgatga
    15181 cgccgcaagg caacattaac tttacccttg agcacatgga aaacgccaaa ggtgtagcga
    15241 tgccggtcgc accaggcgat ggttatactg tgtggctccc ggtcccgcag gatcttgaac
    15301 taaattacgc gctgctgatg cgtaatttct ccggggaaac cacgcgtaac ccacacggta
    15361 agtgattaat ttcgattatt tttcccggat ggaaaattct tagaaaccga tcacatacag
    15421 ctgcatttat taaggttatc atccgtttcg ctgaaaaaca taacccataa aatgctagct
    15481 gtaccaggaa ccacctcctt agcctgtgta atctccctta cacgggctta ttttttatgc
    15541 ataagcccta tccctggtca ccgtcttcca ttgaccacat cgatagaatc tcccttcata
    15601 gcacgatgcc tttcacgtaa cggcatcgtg ctcgcacagg ttccggctaa gcacaaccag
    15661 aacgcgcatg tttgatgctt accaaaaaat attctcactc tccacatttg aatgtcagac
    15721 gagcgacacc atgtaatcct acaccttctg tcttcagctc aactatttgc atttttttgc
    15781 cctgagtaac acagaaatga gttgcatcat tttttactat attttctgca ccagatattc
    15841 tactcctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt ttacgctgaa
    15901 tattactttt atatgcaaga ccaaatttac cgatacttgt ctcatcatta tgcacagcac
    15961 aaccagacat aataaaaata ctaattaatg atatagcagc tatctttttc atctcacctt
    16021 tccccattaa ataccaacga cactctctag tgtttaaata taataatggc tgatcattat
    16081 atttgaatag gattataata aatgttctgt acaacatttc ctacataagt aggacttaca
    16141 gacatgaagg ccctttccag gtaactccat ggggctttaa tatatttgga atagtatatt
    16201 taagatgcca ctgtttggtt gaatattagg catatgctct tttttgaaat ttatcggtgg
    16261 ctaccgttaa tattcgctgt tcccattgca agctcctggt ggtaaccact gaatcctcca
    16321 tacttgaact gactttttat cctccgactt tcatcctgtt ctgactccac cttttctttt
    16381 ctgctctaca ctatctacag accaatcata aaggcacata cgatcatggc agaatttccc
    16441 gccagcttac tgattcttaa tggcaaaagt actgacaatc tacccttgcg cgaagcaatt
    16501 atgctgttgc gtgaggaagg aatgacgatc catgtgcggg tcacctggga gaaaggtgat
    16561 gccgcacgat ttgtagagga ggcccggaag ttgggcgtcg caacggtaat tgccggtggt
    16621 ggcgatggca ccattaatga agtttctacg gcgttgattc agtgtgaggg ggatggcata
    16681 cccgcgctgg gaattttgcc attaggaacc gccaatgatt ttgccaccag tgtagggatt
    16741 cctgaggcac tggataaggc gctgaaactg gcaattgccg gtaacgccat tgcgatagat
    16801 atggcgcagg tcaacaaaca aacctgtttt attaatatgg cgacaggcgg atttgggacg
    16861 cgtattacca cagaaacgcc ggaaaaatta aaagccgcgc tgggtggcgt ctcttacatc
    16921 attcatggct taatgcgcat ggatactctg caaccggacc gttgtgaaat ccgcggtgaa
    16981 aactttcact ggcaaggtga cgccctggta ttggtattgg taacgggcgt caggccggtg
    17041 gcggtcagca attgtgtccg aacgcgttaa ttaacgatgg cttgctgcaa ctgcgcattt
    17101 ttaccggcga tgaaattctt ccggctctcg tatcaacctt aaaatctgac gaagataacc
    17161 cgagtattat cgaaggcgct tcgtcgtggt ttgatataca agccccacac gaaatcactt
    17221 ttaatcttga tggcgaaccg ttgagcggac aaaactttca tattgaaata cttccggcgg
    17281 cgttgcgttg tcgattacca ccagattgtc cattattgcg ttaatcaaac tattctctct
    17341 tgaaccaggg agaatccgaa atgccggatg cggtgtaaat accttatccg gcatacagaa
    17401 caatactaca ccgtaattaa ttttacccgt gtcttctgca atgccagttt atccacctct
    17461 gatacagcat catcggtaat aataatgtca attttttcga ttggcagtac ctgattaaaa
    17521 ccgcgacggt taaatttcga tgaatcgaga acggcaatca ctttattagc ggctgtgacc
    17581 atcgcaccgc taacggaata accctcatta aaggtcgtaa taccattaac cgcatcaata
    17641 ccatcagcac cgacaaacat taaatcggca ttaatatcct gcagggaacg ttcagcaata
    17701 gatccatgca tagatcgcgt tttatggcgg accgtgccac cgcagacgac cagagtaatg
    17761 tctttatttt cggaaagggc aaacgctgcc gggagactgt tggtaatcac cgtgatattt
    17821 ttagcggtca ttaatccttc ggcgataagc attgtggtac ttccgctgtc gagaataacg
    17881 gtcatccctt catggatcat tttgactgcc gcctgcgcta ttctgttttt tggcgcgctg
    17941 gcgagctgaa atcgctcttt aaacccgact tcctgggtct cggtttcact attaccggac
    18001 attattttcg ccgcaccgcc atgaaagcgc gtaaccacgc ctttttgttc gagaaagcgc
    18061 aaatcggcac ggattgtcgc ttccgaggca gcaaatactc ccgccagatc ctgaacaagc
    18121 acggttccct gttcattcac taattggatg atcttattcc ttcgctcgaa tgaattcatc
    18181 gtcatttacc catattcaat tgtggctagt gtaaacgaag ggggaccatt ggtgaatgat
    18241 ctgttttgca caataccggg ccagcatcac gctggcccgc ggtttcatgg aatgagcaac
    18301 actttgcccg gcatggcatt acgagcgatg tcacgcaccg cctgggtgaa gctttcaaag
    18361 cttccacggt gagcgattaa tggctccagg cttaacttac gttctgtcag caaccggctc
    18421 gccgtttccc actcctgccc cggccaaggg ctggaatagt tcatccaact gccgataacc
    18481 gtcagctctt tacgcaatat tttgccaaac gttgccgatg ttaaatgcag atcctgatgc
    18541 aacgtgccca ccagcgccag ttgggcatga ggaccggcaa tctctaccgc cagttcgaca
    18601 gtttgcggta cgccagccgt ctcgaggata agctgattaa aacgcagttc gcgtaaaacg
    18661 ctctgcattt gcggcgcgct catttcgcta ctgttaaatg tttgcatcga accgaaagat
    18721 tttgccagtg ccagtttttc tgagctaatg tcgatagccg tcacactctt tgctcccagc
    18781 gcgacagcgc actgaatagc cagcaggcca atggttccgg caccaataat aataacgttt
    18841 ttattctcac aaccttgcgc taaatgaaaa gcatgcagac caatggtaat cggctcaata
    18901 aaagccccat cctcaatagg catatccgtg ggtagagcaa agacattttt tcgcttaacg
    18961 acaatatatt cagcaaatcc accatcacgc cgcgagccaa taaaatcata ttttgcgcac
    19021 tgggaataaa accctttcag acactctgga caagtaaaac agggtaataa cggcacacag
    19081 gcaaccgcat cgccagggtg taaatcatca acaccggatc ccaccgcatc aatatagccg
    19141 ctaaattcat ggcctaacgt tattggataa taatgtgcac catttttaaa tatcctgggt
    19201 aaatcggaac cacataagcc cgagctggca atttttaccc gcacctcatc ctgatgttta
    19261 atttcaggaa tgacgctttc tgcaacgcgc acgataccat cagtatcatt caccactgat
    19321 ttcataaaaa ctcctgattg ttaaggggga taaactcccc ctcagttaaa aattattctg
    19381 cgagaacggc tttctcttgt ttaataaaac cacgcgctct acgccaggta atgaaaatac
    19441 cggtcagata aattgcgccg ataataatga aaccgggaat attttgcggg gagaaaacct
    19501 gaatcagtaa ccaggtaatg ggagaaccgc cctgatccat tgaagccacc atacccccgg
    19561 cttttaacgc cccagcatta gccgccagtt gggtgtgcaa accaatagtt tgcgtcgcga
    19621 tccacagagt gatgctcatg atgatgacac ccgagattaa ggtgcggaac agatttccac
    19681 gatgcacggc gactgccatc gccacaaaga agccgatggt ggcaagatcg ccaaacggca
    19741 gtacctgatt acccggaaca caaacagcaa ttaaaatggt gagcgggata aaaatcaggc
    19801 ttaccgatac caccgccgta tgccccagca gtaatgcggg atccaagcca atcaggaact
    19861 cctgaccgcc gaacttcgcc tgtaaacgac tacgggcctg cttagcgatg ggcgttaaac
    19921 catccatgat gggtttaatc acccgtggca ttagcagcat cactgccgcc gtttttaccg
    19981 ccagctgcaa tacacctttg acatcgtaac ccgcgaggat gccgataatc agccccatca
    20041 caaaacccac ggtgacaggt tcgccaaaag ggccaaattt gcgctgaata tcatcggcgc
    20101 taaatttaat tcggttaacg cctgggattt tctcgatgat agcatcgacc agcaccgcaa
    20161 tcggccccat atacgccgac gtaccgtgcg gaatagcaat accttccagc tcaaagaaat
    20221 ttcgggtatc gcgggcaaac cagtcgccga gcttataaac aaacgccgcg tgaattacca
    20281 cacccgccat ccctatcatc catgaaccgg ttgccagatg cagcaacgcg ccggtgaaag
    20341 tcatatgcca gatattccag atatcaatat ttaccacccg cgtcatacgg gtcagtaaca
    20401 tcgccacgtt aaccagaata gcaatcggaa tcgccaccag cgcaatttgc gacgcccagg
    20461 tcattggtga agagcccggc cagccgacat cgaccacatg cagattcagg tcgaaatttt
    20521 ccgccatcgc tttcgccgcc ggaccaatgg aatccagcat taagccaatc acaaggccaa
    20581 tgccaacaaa gccaatccct atatgaagac ccgctttaaa gcaatcgcct gccttcatgc
    20641 ctaatatttt agaaaaaata atgatgacaa tcggcagcat caccgtaggg ccgaggtcga
    20701 gaatataacg catgacttct gaaaacatag gtcacccctg taagatagtc agaattttat
    20761 tttgtaatgc ttcgataccg acaccagaaa caaaaggcat gccgtgaact aacggaatat
    20821 cgccaaaact acgatccacc ctggcagtag tgcatatcaa atgcacgcca tccatatagg
    20881 tttctatttc attaacccga cactggatta attcaacagg aatattatga ttctgacaca
    20941 attctttaat ttcttccgcc gccatcgtag aggtcgcaac cgcgcctccg caagcgacaa
    21001 taatcttgcg tttcataaat agtccttttt attgttacag agggatttat tcgtctgaat
    21061 ctaaaacata ctttgtgaag tattccttta actgggtttc aggaagagtg attagtgtct
    21121 cgacgatatc gggctgttgt aacttgccaa ataaacagcg taaaagtttc aattgctgct
    21181 gcggattttc cacaatcaac gcaataacca acgatacctc cacgtcgtta tcatcatccg
    21241 cttgctgaaa atgaacttta tttgttggcc ttaacagata aatggctgac gacttagcat
    21301 gaatcgcctc acaatgcggt attgcaatgg cgtgctgctc aagcattatc ccggtaggga
    21361 attctgcttc tctggcaatt aatgcctgtg gccaggtatc atgaaccaca cctttggcga
    21421 gcatctcatt accgatatgg gttaaaactt cgctacgatc gacaaaagaa attccgctac
    21481 gaacaaacag gttagtcata taccgtcctt attccgcaca gccgtagcga taggcgcgca
    21541 aaacatcata aattttatcc ataatcagct gatgcggtat tgctgataat tccccggact
    21601 gaatgcgttc aaattgtttg ggaagatact gactaatcat gcccagtggg atgtccacgc
    21661 cttcaagatt caccatcatc gtttcgacgc tatttttaat ccgactatgc ggccagtaat
    21721 aacgaatacg atccgacagg ctgtaacgaa tatccagtaa tgaatcgtta aaacccgtac
    21781 gataatattt tttccagtat tgcggttcgt cgagcatcac ttcttcaatt accgccaggc
    21841 aaccgctgcg attttcaggg gcgataagtt cctgctcaat ttgtgccagt gcaaatatcg
    21901 cctcgcgtaa agcaaaggtt aatgcgggac cgactttcaa tattgcaaag tgatcgcgga
    21961 ctaattccca ataagccgtc cgggtctggt aatcggtaga atgtgcttca taaaccattc
    22021 gggtgttttc tatccattgc gccagcggct gcgcttcctg cggctgataa tggataatat
    22081 tgctgtgatc aaattccacg cccggctgca ccacgatggc aatcacgcgt gttaacgcct
    22141 ctgtcagccc acggacaata aaggcctttt gatgcgtacg taaagtattg gcggcatctt
    22201 caacatgggt gatgtgtact gactgaatgg cgctggcctc accgcccgga accggaactt
    22261 cggtgccaat gacatagctc agttgctcac gctggcaatc tgtcgccaca ctttccgcag
    22321 caaagcaaag cacagcagct cgttccgcaa ccgtttctgg tgctaacggt atgggatccc
    22381 ccgcgcagga cattgacgca tcaagatgaa ttttactgaa gccggcacga acatattcct
    22441 ttaccagctc gacggatttt tccatcgccg catccgcatt ttcttgctgc cagcagtttg
    22501 gccccagatg atcgccgccg agaataatgc gttcgcgtgc aaacccaact ttatcggcaa
    22561 tcgtaaaaac aaattcgcga aagtctgccg gtgtcattcc ggtataaccg ccaaattgat
    22621 tgacctggtt tgacgttgct tcaatcagca ctttgcgcgt gctgttgcga tcaaatgcca
    22681 gcgccgcttc gataaccaac ggatgggcag aacagactga acatatgccg atatgttcac
    22741 cagctttatg ccgggcaatt aacgttttca tgttttttcc ttattaaatg gcaggtgcgt
    22801 tatgccctgc cctcgcagcc acaatcggca atcactttgc tcaccacatc gcgcattgcg
    22861 gatttagccg actgcaaata atcccggggc tcggtcgctt cagggtgctc ggtcaggtaa
    22921 ttttttaacg cctgcgagaa ggcatttttc agctccgttg caacgttgat tttgcatatc
    22981 cccagtttga tggtttgctg aatatcctta gtcgataacc ctgacgcgcc atgcagcacc
    23041 agcggtaagt tcacccactg gcgaatgttc tccagtctag aaaaatcaag cgccggtgcg
    23101 ctggcataca tcccatgagc cgtgccgatg gcgaccgcca gggaatcaat tccggttgcc
    23161 tcggcaaatt cacgcgcctg agcggggttg gtataaaaag catcggcttc attgacttgc
    23221 acatcatctt cctggccgcc aagttgcccc agctccgctt cgacgctgac atcaaagcga
    23281 tggcaaaaat ccaccacctc tttgacccgt gaaatatttt gcgcaaaagg caaatgcgag
    23341 gcgtcaatca tgactgagcg cacgccagaa cgaaccttct gagcgatatc gtcaaatttc
    23401 gtgtgatggt cgagatgaat tgccagtgga tggtgatatt gcttcgccat cgcgctgacc
    23461 agcgccaaca gattttctgt accagcatga gtaaatgtgc caggcgttcc ggcgatgatg
    23521 accggcgcat gcaggttggc agcggtttcc accaccactt gcatcgtttc gagattgtga
    23581 atattgaatg ccggaaccgc ataaccgccg cgctgtgcgt tgttcagcat ctgctttgtc
    23641 gataccacgt gcattttcat atcctgtcgt ttgttttcgt tttcaaaata taatgaaatt
    23701 atttgttttt aaatatcgag ataacgatca caaaaacgac aatatgaaaa ttattcgagg
    23761 agtgaaaggc aaaaaaacgg cctcccgata gggaagccgt agcaaagtgc gcgtgttttt
    23821 atgccggatg cggtgtaaac atacgtggca gtgctatgta ggcatgataa gacgcttccg
    23881 cgtcgcatca ggcataggtt gccggatgca gtgtaaacgc cttatccgtc ctacggggtg
    23941 gtgttgttcg tataaggcgt atcaggcaat agtaattttg ctatcgagat aaacgtcctg
    24001 cacggcgtta atcagtttca cgccgtcagc catcgatttc ttgaacgcct tacgtccaag
    24061 aatcagcccc attccgccag cgcgtttgtt gataaccgca gtacgcactg catcactgag
    24121 gtcagtttca ccgcctgcag caccgccgga gtttatcaac ccagcccggc ccatatagca
    24181 gttagctaac tgataacgca ccagatcaat cgggttttcg ctggttaact tgctgtacac
    24241 gcgatcgtcg gtataaccgt aattaatcgc tttatagccg ccgttatttt ccgccatttt
    24301 ttgtttgacg atatctgcgc cgatggttgc cgccagatgg tttgcctgac cggtcaggtc
    24361 ggcggaaaca tggtaatcaa cgccatcttt cttaaaggcg gagttacgca aataggccca
    24421 cagcacggtc accatgccca gctcatgcgc acgttcaaaa gccgcagaaa tttcttcaat
    24481 ctggcgacgt gactcttccg agccaaaata gatagtcgca ccaaccgcca ccgcgcccat
    24541 gttgaaagcc tgctccacgc tggcatacag cgtttgatcg taggtattcg ggtaacttag
    24601 cgtctcgttg tgattaagtt tgacgagaaa tggaatgcga tgcgcatagc gccgcgatac
    24661 cgacgccaac acgccgtaag ttgataccac acagttacag cccgcttcga tcgccagttc
    24721 aacaatgttt ttcgggtcaa agtagagcgg gttagcagca aatgaagctc cggcagagtg
    24781 ctcaacgccc tggtcaaccg gcagaataga aagatatcct gtgccagcca gacgcccggt
    24841 gttgtacaac gtctgcatat tacgtaacac cgctggcggg cgattattgt caatcattac
    24901 gcggtctacg tagtcatgtc cggggagata aagctggtca gaaggaattg tcatacaacg
    24961 gtgctgtaaa aggttgtcgg cgtctttgcc aagcaactgc gcaatatctg tcattactat
    25021 gctcccgtaa attccgattg gatatcggct atggattgtc ctggcccgcc ttttgcgggc
    25081 aatcataatc ctggtcgtta cgggcgagtt tttccatctt ttggatgttt tttcagcgtt
    25141 ttctgttggc tcgattcatc agaaaaaatg ttagcgcggt caagttttca ccgtaaaggc
    25201 atttaaaagg tattattaag tggtattgtc atcgcgtacc ttacattacc tgtcatgaag
    25261 gaattaaaag atgaaaacaa cagcaaagct gtcgttcatg atgtttgttg aatggtttat
    25321 ctggggcgcg tggtttgtgc cattgtggtt gtggttaagt aaaagcggtt ttagtgccgg
    25381 agaaattggc tggtcgtatg cctgtaccgc cattgcggcg atcctgtcgc caattctggt
    25441 tggctccatc actgaccgct ttttctcggc gcaaaaagtg ctggcggtat tgatgttcgc
    25501 aggcgcgctg ctgatgtatt tcgctgcgca acagaccact tttgccgggt tctttccgtt
    25561 actgctggct tactctctaa cctatatgcc gaccattgcg ctgactaaca gcatcgcttt
    25621 tgccaacgtg ccggatgtgg agcgtgattt cccgcgcatt cgtgtgatgg gcactatcgg
    25681 ctggattgcc tccggtctgg catgtggttt cttgccgcaa atactggggt atgccgatat
    25741 ctcaccgact aacatcccgc tgctgattac cgccggaagt tctgctctgc tcggtgtgtt
    25801 tgcgtttttc ctgcccggca cgccaccaaa aagcaccggc aaaatggata ttaaagtcat
    25861 gctcggcctg gatgcgctga tcctgctgcg cgataaaaac ttcctcgtct ttttcttctg
    25921 ttcattcctg tttgcgatgc cactggcgtt ctattacatc tttgccaacg gttatctgac
    25981 cgaagtcggc atgaaaaatg ccaccggctg gatgacgctc ggccagttct ctgaaatatt
    26041 ctttatgctg gcattaccgt ttttcactaa acgctttggt atcaaaaagg tattattgct
    26101 tggtctggtc accgctgcga tccgctatgg cttctttatt tacggtagtg cggatgaata
    26161 tttcacctac gcgttactgt tcctcggtat tttgcttcac ggcgtaagtt acgattttta
    26221 ctacgttacc gcttacatct atgtcgataa aaaagccccc gtgcatatgc gtaccgccgc
    26281 gcaggggctg atcacgctct gctgccaggg cttcggcagt ttgctcggct atcgtcttgg
    26341 cggtgtgatg atggaaaaga tgttcgctta tcaggaaccg gtaaacggac tgactttcaa
    26401 ctggtccggg atgtggactt ttggcgcggt gatgattgcc attatcgccg tgctgttcat
    26461 gatttttttc tgcgaatccg acaacgaaat tacggctatc aaggtcgatg atcgcgatat
    26521 tgcgttgaca caaggggaag ttaaatgaaa acagaacgta ttctcggtgc tctttatggg
    26581 caggcgttag gggatgcgat ggggatgccc tccgagcttt ggccacgcag ccgcgttaaa
    26641 gcacactttg gctggattga ccgttttctg cctggaccaa aggagaataa cgcggcctgt
    26701 tattttaacc gcgccgaatt caccgacgat acctcgatgg cgctgtgtct ggcggatgcg
    26761 ttgctggaac gtaaaggcaa gatcgatccg gatctgattg ggcgtaatat cctcgactgg
    26821 gcgctgcgtt tcgacgcctt caacaaaaac gtgctaggtc cgacctcgaa gattgcgctt
    26881 aacgccattc gcgacggtaa acccgttgct gaactggaaa acaacggcgt aaccaacggc
    26941 gcagcgatgc gcgtctcgcc attaggttgt ttgcttccgg cgcgcgatgt tgattccttt
    27001 attgatgatg tggcgctggc gtcgagcccg acccataaat ccgatctggc agttgcaggt
    27061 gcggtagtca tcgcatgggc gatttctcgt gctattgacg gagaaagctg gtcagcgatt
    27121 gttgattcac tgccttcaat tacgcgacat gcccagcaaa aacgcaccac cacgttcagc
    27181 gcctcactgg cggcgcggct ggagattgcg ctgaaaattg tgcgcaatgc cgacggcacc
    27241 gaatccgcca gcgaacagct ttaccaggtc gttggcgcag gtaccagcac tattgagtcc
    27301 gttccgtgcg ccattgcgct ggttgaactg gcacaaaccg acccgaaccg ttgcgccgtc
    27361 ctctgcgcta accttggcgg cgatacagac accatcggtg ctatggcgac ggcaatctgc
    27421 ggcgcgttgc atggcattaa cgctatcgat cctgcgttaa aggcggagct ggatgcggta
    27481 aatcagcttg atttcaaccg ctatgccaca gcgctggcga aatatcgtca acaacgggag
    27541 gcggtatgag cggcgctcga ttacacacgc tgctgcctga attaaccacg cgtcagtcgg
    27601 tgatggtcgt cggcgcggcg gtcattgacg tgatcgccga cgcttatgcc ctcccctggc
    27661 gtgggtgcga tatcgaactg aaacagcaga gcgttaacgt tggcggctgc gcactgaata
    27721 ttgccgtggc gttaaagcgc ctcggcatcg aagcgggtaa tgccttgccg ctcggtcagg
    27781 gggtatgggc ggagattatt cgcaaccgga tggcgaaaga ggggttaatc agcctgatcg
    27841 ataacgccga aggcgataac ggctggtgtc tggcgctggt tgagccggat ggcgaacgta
    27901 cttttatgtc attcagcggt gtggaaaatc agtggaatcg ccagtggctg gcgcgattaa
    27961 ccgttgcgcc tggcagcctg ctctattttt ccggttatca actggcctcg ccctgcggcg
    28021 aattgttagt ggaatggctg gaagagctgc aagacgtgac gccgtttatc gattttggcc
    28081 cacgtattgg cgatatcccg gacgcattac tggcgcggat catggcctgt cgacctttag
    28141 tgtcactcaa tcgtcaagag gctgagattg ccgccgaacg ttttgcttta tccgcagaga
    28201 taacaacact tggcaagcaa tggcaggaga aatttgccgc gccgttgatc gttcgtctcg
    28261 ataaagaagg cgcatggtat ttcagcaacg acgcttctgg ctgcattccg gcatttccaa
    28321 cgcaagttgt cgacaccatt ggggcgggcg acagtcatgc cggtggcgta cttgccgggc
    28381 tggcctctgg tctgccactg gcggatgccg tattactggg caatgcagtg gcgtcatggg
    28441 ttgtcgggca tcggggcggt gattgtgcgc caacgcgcga ggaactactc ctcgcacaca
    28501 aaaacgtata gatcgctgcg acagtggcta atgctgtact caataggccg ttgctgttgg
    28561 tcaagcgcca cttgcttgat caccagcacc ggtattttgc tatctaactg aatatgtgac
    28621 tgaaactcgg catccggcat ccgggcgcta acgcgggaac gcgtacgttg tgggtaaatg
    28681 tgctggctgc ggaaatagtc ataaagtgaa atcccgatgg catcaacatc atgaattaaa
    28741 tgcgccggaa cccacgattc ctcaatcgat accgcttctt catcgacata gcgaatacgc
    28801 ttaagcaaaa agacatcact tcctgcttcg accgccagct gctgcgcgac ttcttcggga
    28861 catttcacga cacgcttgtt aacccatagc gtatcgggct ttttcccgcg caataccacc
    28921 tgctgagaaa aacctcgcgc ttctttcaac gagtattcga agatgttgtt gatctgcgtg
    28981 ccgtaaccgc gcgaacgcgt caccacaccc tcttcttcca gcgcctgcat cgccttgcgc
    29041 accgtaatgc gcgaaacgcc ggttaactga cttaaatcac gctcaccggg caaaatattg
    29101 ccatgctcca gcaccccgct gcgcacggca ttttttaccg tttcggcaaa cttaatataa
    29161 agcggcgtgt tatccgccgc taaaatacgc tcgttcagtt gggcaatcag ctgggtatgc
    29221 gcttgttcca tttgtcttgt tcctgccggg gagtctggtg ggagtatatc actgcttctt
    29281 atttaaggcg tttctttaat cccatcaacg aatgcctgca actcctccac cgtgccatta
    29341 aatacattaa aatccaccgg accattgatg ccatctacct gtccacggtc ggaatgctgc
    29401 cagaagcgcc aggccatgcc gtcattgtcc ggacgacgtt gataatagtg agccacccac
    29461 cacggatatt cattgaaata gcccgccaga ttggtgtgat aaaaaacggc tcctgagtaa
    29521 ataatcggct tttttcccgt acttttttcg accattttta gccactgact tactcgtttg
    29581 cgtaattctt ttgccgataa ttttccccgt tcttctacgt ccagtaccgc agggaaatca
    29641 ccttgtgaga aatccaccgt ttgcagaaat aatcgtgcct gaaccggcgc tgctaccgac
    29701 ggggagaaat aatgatacgc cccgcgcagc aggccatttt cgcggcttag ttgccagtta
    29761 cgcgaaaaat acgggtccac cagcttttcg ccttccgtcg ccttaataaa agcaaactgt
    29821 aagcggatac cgttgtcgcg catttttgcc acccgctgcc agtcgatccg ctcctgccag
    29881 cgagaaacat ctattccgtg aatggtgtaa ctggcaggaa tgcgaatagc gaaagattta
    29941 accgggcgat aaccgtagaa atggatgtag tctgtaatct gaactgctgt cgaatagaaa
    30001 aaattcacct tctgacgcgg ataaattgca acgatggaaa ttagcccaag agcgcataat
    30061 agagaggtga gtttttttcg actggtaatt cttagctgca ttggtttcat ccctgaatgt
    30121 cagtgccaaa ggctgacaat aaccaaagca gctatagtac ggtgctcacc accaggcgtg
    30181 gaagtggtga accggaccaa taccgtgacc aacttccagc gtgtcggcct gggctaatgc
    30241 tgatgaaagc cagctttttg cctcctgtac ggtgtcagcc cagtttgtat ggcgcgggcg
    30301 tagtgcagcc aacgccgcag agagcgtaca accagtgccg tgggtgtttt tggtcataat
    30361 gcgcggtgcg gtaaaccgtt gttcaccctc gcgggtaaac agccagtccg ggctttgctc
    30421 atcatccaga tgaccacctt tcattagcac tgcgccacag cccatcgcca acagcgatcg
    30481 cccttgttcc agcatttcct gttcagtgcg cgcgtgtggc gcgtcgagca aggcggcagc
    30541 ttcgggcaag tttggcgtta ttaatgaaac ctgtggcaat aatcgactgc gcagcgtagc
    30601 aaccgccgaa ggtgaaagca gcgggtcgcc gctttttgcc agcataacgg tgtcgagtac
    30661 cacgtattgg atctgataac gttgcaaccg ttctgccacc gcttcaacaa tatcggtttc
    30721 cgccaacata ccgattttag tggtgtcgat tcgcacatcg ctgaacaccg aatcgagctg
    30781 ggcggcgaca aaatcagact caatgcgata caccgactgt acgccacggg tattttgcgc
    30841 caccagtgca gtaataactg agcaaccata agcgccaagt gccgagaagg ttttaagatc
    30901 ggcctgaatc cccgcaccac cactcggatc agtaccggca atcgtcagag cgttaattcg
    30961 tttcatgcct gcacctcctg cgtcaattgc cagagcgcat caaggaaatg tggaacaaaa
    31021 ctgcctggcc cctcgcttct ggcgactgcg cgttctccgg cttgtttcat ccagtgacag
    31081 gcagatgcga cattttccag cgtatcgcct ggtaacgcac agcaggcagc gacaaccgcc
    31141 gataatgcac agccagttcc taccactttg gtcattaacg gatcgccacc gtgaatacca
    31201 atgatacgat gtccatcggt aacataatcc atctcgccag tgaccacgac gattgcgcca
    31261 gtttcccgtg ccagtgtttg tgcagcgggt atcgcgttag ctgcggcgtc agtggtatcc
    31321 actccccgtc caccattagc aatgccagct aatgccatga tttccgaagc attaccacgt
    31381 atagcggttg gcttatgagg cagaagttcc agacaaaaac ggcggcgata atcgagtgca
    31441 ccaaccgcta ctggatcaag cgtccagggt gtttgagagc tttttgcttg ctcaacggcg
    31501 gcacacatcg actgtgcgcg tagctgcgtc agcgtgccaa cgttaatcag caacgcacta
    31561 gcaatagccg caaactgact ggcctcttcg gtttcgataa ccatcgctgg cgatgcaccg
    31621 agcgccagca aggtattggc agtaaaggtt tgcaccacat cgttggtcat gcagtgcaca
    31681 agaggggaat gttggtgaaa aaggtgtaac gcgtgcgcag attgcgctga actctgcagg
    31741 tcgacttgca tagtttgctc ctgccataac gtgaagaagc aatgacctgg tggtccgtga
    31801 cttccctacg ctggcattat ccagatcagg taatacgggt atttctcagc cttcacaaag
    31861 aagggcaccc cgagtcattt aaaacaaaat caaaagattg cgattaacgt cccgttaatc
    31921 cctgttctgg atcatgccag cggaaagcca aagactcaac cctaaaaaat cctctgtgtc
    31981 ccagtttgtg tctcacgttg tggcccacag ataaatggca ggcacaaaaa aaccgcccat
    32041 aaggcggctt aagtaaatta ttcagtcgtg aagatttaaa tgaaatctat tttctgcata
    32101 cgctttcttc atgcagtcga agtgcttttg ccaattccaa ttggaatgca aaggaaagac
    32161 gggaacggct gatcttgata ccgtcaataa tgatgtatgt agggtaaaca gtgaccatgc
    32221 ggcccaagta ttggaagtgt ttagggtggt ttactttatc gttattcatt gtgtgccatc
    32281 cgtctgggtg ggggtaatcc cggcaacgga tgattgatct aaaatcgatc aaaatcaata
    32341 acttaacaga tcaccaaatc caggggggtg tacattggcg agcctccctc agcctaacct
    32401 atacacgacc ataaaatagg ggcggattcc ttccagttgg gtgggaaaat atctgggagt
    32461 ttggatacag tagccaggca gggtaaccag acccatagat gagaatgttt attattccca
    32521 aatatccagg cgaggggcct gctgtggctc tctttacgcg ccgctgtggc gtcttctctc
    32581 ctctttctat acaatcccat agctaaccca ttgcgaggct tacagagcgt tacagagcgt
    32641 ccgtgtgaat attgcataaa tgaaagtggt gaaaggtccg atttggtcaa cttttagaca
    32701 actatttaac atatgcgcaa caggttagac ctgtaccctt tcaataggtc cgcctcctgg
    32761 gcgctgtgta cgccccataa ctgtggctct tctgtaccca tccgggagcc attccacaga
    32821 gtaaactgta aaactgatct gcctgtgagt aacgcccttt tcctgtggat aacttccacg
    32881 cactaacccc ataaactccc cttttgaggc gtccgcctct tcaggccctt tcttgtggca
    32941 aaaacacaaa aagggtttag ggcggtggct accgcccctg tagatgggaa tgagtcgcac
    33001 tcgaaagagg gattgagaac caattctcaa gtgataatcg atctcatttg tgttcgaagt
    33061 tttccccaga aaaataattt tggggttaag catgtaaaat gctacacact tgcactttgt
    33121 aatgataatt aattgaccat aaaaacaaat agttagaagc gctgggggag tcccactagt
    33181 tacacaattg attgcttttt gagctaaaac tgattctttt accgggtata caatccattt
    33241 gatcgccctt taccacatat ttaaacggat ttaaagccat ttatttaggg ggttagcaaa
    33301 gtgttgattc catggttaca gataacaaaa aagcccgccg ggggttagcc gggggcctgt
    33361 ggttgggggt gtcaaacgtg gttagttcgg cccgccctgc attagcatgt tgaaagcctg
    33421 ctgattattg ctctcaactt cagagcgtac cagatcctta acctatccat cctgcacttt
    33481 cacattaacg ttcacgttta gcggctggtt tagccacgct ggcggcgtca tcagtggatt
    33541 acctgccata tcaactttaa gctgtggcgc tgtaatcgtc ggcgtaggag gtacataagt
    33601 tggattggag taagagctta cccaccctgt tttatctgct ggtttggcaa agtctggatt
    33661 agtcggaagg tctggactat ggaggaaatc ccacagttta cccagcgggc tatctttttt
    33721 cagattgccc ccggcggtgg tgtcctgtcc atacatggcg tcgttagcgt catgacctta
    33781 tcccaggcat aatccccacc taccgaaccg accgcaatgc tccagccaac aggaccgcca
    33841 gagagagccg ccgcgccgcc taccttgcca actttcggac cccgacccgg tttgtttggc
    33901 ttgtctttac cttccccacc agatccgcca gtagtagcgg cttcaatgcc accaatagcg
    33961 gcgatccctt tcatgacccc cagtaatttg gttagtgggt taagcatggt caacaaccat
    34021 ttaaaggcta cgcccaactt ccagatagac ccggcgaaca tagccacccc ggcggcgtat
    34081 gctgccattt cgccccaggc tttaagctgc tcaccagtga tccccatctt gctggcgtaa
    34141 tactcaataa tgcggaaggt caggatcaca gtgtcatgca actctgtaaa cgcatctgtg
    34201 gcaccctcaa cgattttccc ggcaatatgc ccgaacatct caaaggcccc accagagccg
    34261 ttaacagcgg cgctgatagc gtcgaaagcg ctggttaatt cgttgccgaa gccagaagca
    34321 aagaagatgt tcatcgcgtt ctgcatactg gtttgcaggc gctgccagct tgcccggttg
    34381 gacttcatag ccttatccag agcgccgccg ttacgagctg cggctttcat ctgtttagcc
    34441 acttccggga ggatgtcctt agagaacagc ttcccttgtt gcatcaggtc aaacagatcc
    34501 ttttctgtaa ggttggttgt ccctttcatc tggttgagag ccttaacgaa cagacccaca
    34561 gcccccggga gagcttcgga caactggcct ttgagttctt cactcattac cgttccttta
    34621 gacatcatct gattcaaact tttcaaagcg cggctggctt catcggctga agcacctgta
    34681 acaaggcccc attcactggc accctcaaag atggatcgca tctggtcttt agggagcttg
    34741 tcaccaacag cggcggcaaa ctgagtgtaa tcacgggaag tcttgaggag atccagcccc
    34801 aaacggttgg attgatcacg gagataatca aactctttag cggccttatc tgtggaaccg
    34861 gtgatcgctg tcagaccaga aagcgcggat tcaaaatcct gtccggtggt catgatcttg
    34921 ccgctcagcg ccacagttgc ggcaatggtg atcgccccgc ctgcaccctc gatcatgg
//
LOCUS       DAJVOY010000023        34545 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVOY010000023 DAJVOY010000000
VERSION     DAJVOY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.023"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            114..720
                     /locus_tag="OQL90_000981"
                     /pseudo
     CDS             114..720
                     /locus_tag="OQL90_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            823..1325
                     /locus_tag="OQL90_000982"
                     /pseudo
     CDS             823..1325
                     /locus_tag="OQL90_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1392..2849)
                     /gene="nuoN"
                     /locus_tag="OQL90_000983"
     CDS             complement(1392..2849)
                     /gene="nuoN"
                     /locus_tag="OQL90_000983"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1367838.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2856..4385)
                     /gene="nuoM"
                     /locus_tag="OQL90_000984"
     CDS             complement(2856..4385)
                     /gene="nuoM"
                     /locus_tag="OQL90_000984"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1367839.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4549..6390)
                     /gene="nuoL"
                     /locus_tag="OQL90_000985"
     CDS             complement(4549..6390)
                     /gene="nuoL"
                     /locus_tag="OQL90_000985"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1367840.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6387..6689)
                     /gene="nuoK"
                     /locus_tag="OQL90_000986"
     CDS             complement(6387..6689)
                     /gene="nuoK"
                     /locus_tag="OQL90_000986"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1367841.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6686..7240)
                     /gene="nuoJ"
                     /locus_tag="OQL90_000987"
     CDS             complement(6686..7240)
                     /gene="nuoJ"
                     /locus_tag="OQL90_000987"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1367842.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7252..7794)
                     /gene="nuoI"
                     /locus_tag="OQL90_000988"
     CDS             complement(7252..7794)
                     /gene="nuoI"
                     /locus_tag="OQL90_000988"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1367843.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7809..8786)
                     /gene="nuoH"
                     /locus_tag="OQL90_000989"
     CDS             complement(7809..8786)
                     /gene="nuoH"
                     /locus_tag="OQL90_000989"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1367844.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8783..11509)
                     /gene="nuoG"
                     /locus_tag="OQL90_000990"
     CDS             complement(8783..11509)
                     /gene="nuoG"
                     /locus_tag="OQL90_000990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1367845.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11562..12899)
                     /gene="nuoF"
                     /locus_tag="OQL90_000991"
     CDS             complement(11562..12899)
                     /gene="nuoF"
                     /locus_tag="OQL90_000991"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1367846.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12896..13396)
                     /gene="nuoE"
                     /locus_tag="OQL90_000992"
     CDS             complement(12896..13396)
                     /gene="nuoE"
                     /locus_tag="OQL90_000992"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1367847.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13399..15201)
                     /gene="nuoC"
                     /locus_tag="OQL90_000993"
     CDS             complement(13399..15201)
                     /gene="nuoC"
                     /locus_tag="OQL90_000993"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1367848.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15295..15957)
                     /gene="nuoB"
                     /locus_tag="OQL90_000994"
     CDS             complement(15295..15957)
                     /gene="nuoB"
                     /locus_tag="OQL90_000994"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1367849.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15973..16416)
                     /gene="nuoA"
                     /locus_tag="OQL90_000995"
     CDS             complement(15973..16416)
                     /gene="nuoA"
                     /locus_tag="OQL90_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1367850.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17047..17985)
                     /gene="lrhA"
                     /locus_tag="OQL90_000996"
     CDS             complement(17047..17985)
                     /gene="lrhA"
                     /locus_tag="OQL90_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1367851.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18904..20121
                     /gene="alaA"
                     /locus_tag="OQL90_000997"
     CDS             18904..20121
                     /gene="alaA"
                     /locus_tag="OQL90_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1367852.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20205..20804
                     /gene="yfbR"
                     /locus_tag="OQL90_000998"
     CDS             20205..20804
                     /gene="yfbR"
                     /locus_tag="OQL90_000998"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1367853.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20863..22695)
                     /locus_tag="OQL90_000999"
     CDS             complement(20863..22695)
                     /locus_tag="OQL90_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1367854.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22782..23432)
                     /gene="hxpA"
                     /locus_tag="OQL90_001000"
     CDS             complement(22782..23432)
                     /gene="hxpA"
                     /locus_tag="OQL90_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCS1367855.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPKVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(23443..23937)
                     /locus_tag="OQL90_001001"
     CDS             complement(23443..23937)
                     /locus_tag="OQL90_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1367856.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(24020..24475)
                     /locus_tag="OQL90_001002"
     CDS             complement(24020..24475)
                     /locus_tag="OQL90_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1367857.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24813..26015
                     /gene="ackA"
                     /locus_tag="OQL90_001003"
     CDS             24813..26015
                     /gene="ackA"
                     /locus_tag="OQL90_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1367858.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26090..28234
                     /gene="pta"
                     /locus_tag="OQL90_001004"
     CDS             26090..28234
                     /gene="pta"
                     /locus_tag="OQL90_001004"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1367859.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28424..29944
                     /gene="yfcC"
                     /locus_tag="OQL90_001005"
     CDS             28424..29944
                     /gene="yfcC"
                     /locus_tag="OQL90_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1367860.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVSRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29977..30519)
                     /gene="yfcD"
                     /locus_tag="OQL90_001006"
     CDS             complement(29977..30519)
                     /gene="yfcD"
                     /locus_tag="OQL90_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1367861.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30577..31128)
                     /gene="yfcE"
                     /locus_tag="OQL90_001007"
     CDS             complement(30577..31128)
                     /gene="yfcE"
                     /locus_tag="OQL90_001007"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1367862.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31184..31816)
                     /gene="yfcF"
                     /locus_tag="OQL90_001008"
     CDS             complement(31184..31816)
                     /gene="yfcF"
                     /locus_tag="OQL90_001008"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1367863.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31952..32599
                     /gene="yfcG"
                     /locus_tag="OQL90_001009"
     CDS             31952..32599
                     /gene="yfcG"
                     /locus_tag="OQL90_001009"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1367864.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32656..33018
                     /gene="folX"
                     /locus_tag="OQL90_001010"
     CDS             32656..33018
                     /gene="folX"
                     /locus_tag="OQL90_001010"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1367865.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33039..33932
                     /locus_tag="OQL90_001011"
     CDS             33039..33932
                     /locus_tag="OQL90_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1367866.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33980..>34545)
                     /gene="rpnB"
                     /locus_tag="OQL90_001012"
     CDS             complement(33980..>34545)
                     /gene="rpnB"
                     /locus_tag="OQL90_001012"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1367867.1"
                     /translation="GYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSA
                     FPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKA
                     LFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQE
                     MLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8662 a         9395 c         8641 g         7847 t
ORIGIN      
        1 tttgtataga tctaacaacc gtaataaaac aaatactcat aactcattat aaataatgat
       61 aaaataacgt cttttattga taatttatat atgggaaaaa ttccttttgt tatattgttt
      121 ctgatttcac tccctctgat tatttttacc aaccctgggt cactgcatta ccaccaacgc
      181 cccgacacgt cagtcctgag caattagaaa aaacagttcg ttatcttaca caaactgtgc
      241 atccacgtaa tgccgacaat attgataatc tgaataggtc cgtagagtat ataaaagagt
      301 tctttgtcag tagcggtgcc agagttaccg cgcagggcgt ccccgattgg ctattacgac
      361 tctacgcctg gtagccagga ttacctttac ccgcaatgtc ctggctttat cccgatcggg
      421 gggactttat tgcctggtcg gcagaataca ggatatcaac gccgttcgtc aggtaaaaac
      481 ggcattgtta tcatcccggg atttatctgt ttattctatg aatgccccat ggtttattcc
      541 cggtattgat ttctctgacc acctgaatta ttggcaacac gatattcccg ccgtaatgat
      601 tactgacacc gctttttatc gtaataaaca ataccacttg cccggtgata cagcagacag
      661 attgaattat cagaaaatgg ctcagatggt gttataacct tgttacacaa cagtaaataa
      721 taaaaatttc tcgaggcggg tccagaaaca gcgacatgta cattttattt tccctcgtct
      781 gttttaagat gcgaaacata aaattcaatg gaggaaaaat aaatgggaat gattggttat
      841 tttgcagaaa ttgactcaga aaagattaac caatctgctt gagtctactg aaaaaccttt
      901 gatggataat atacatgaca cgctttcagg attacgccga ctggatattg ataaacgctg
      961 ggatttttta cactttgggc tgacaggcac ccctgctttt gatcccgcga agaatgatcc
     1021 gctgagtcgt gccgtgcttg gtgaacacag cctcgaagat ggcatagatg gcttcctggg
     1081 attgacctgg aatcaggaat tagccgcgac aatcgaccga ctggaatcac tggatcgaag
     1141 taagctgcgt aaacagtttt ccattaagcg actatgaaat ggagatttat ccgggagtga
     1201 ccttttcaga agagttagag gggcaactct ttgcctcgat catgcttgat atggaaaagc
     1261 tgattagcgc gtatcgacgt atgctgcgac aagggaatca tgcgctgacg gttattgttg
     1321 ggtgatggtg tcagagcgcc aaaaaaaacc gccgaacatg tcggcggttt tctgacttac
     1381 aaactaacag attacatcag cggcattgct aaccgcacaa tactaatcag cgattgtggc
     1441 catacaccca gcaccagtac caacagtgca gaaatcagca ccacgatacc gcccgcgctg
     1501 tactgccagt ttgacggtgc atcgcgaccc ggttgttccg gtgcgtgcag gtacaggctc
     1561 accgccacgc gcaggtagta gtagaggccg attgccgaac cgacaaccac ggcacccacc
     1621 agccaccaca agtgtgcctg gacaccgacc gccagcacgt agaacttacc gataaagccc
     1681 agcgtcatcg ggataccggc cagagacagc atcatcaccg tcatcactgc cgcgaggatc
     1741 ggacgatgcc agaacagacc gcggtaagag aacagggaat cagcatccgg gccacgatac
     1801 gggctggaca tcaggctgac cacgccgaac gcgccgaggc tgctgaacag ataaccggcc
     1861 aggtaaaccc ctaccgcttc catcgacatc tcgccggttt gcagcgcaat cagcgccacc
     1921 agcagatagc cgaggtgaga gatagatgag taaccgagca gacgtttgat gttggtctgg
     1981 ctcaacgcca tcaggttacc gaagatgatg gaggcaaagg cgataatcgc cagtactacg
     2041 cgaatcgctt cgctgtcacc caccggtgca tagaggaaca gacgcatcac cacaccgaag
     2101 atagcgattt tgctcgccgt cgccaggaaa gtggaaaccg gcgcaggcgc gccttggtat
     2161 acgtctggcg tccacaggtg gaacggcacc agagagagtt tgaagccgag gccaacaatc
     2221 atcatgccga aacctgccag caacagcggc tcgttgagca taccgtcacc gaggttttta
     2281 cccagcgcga caaacgacag gtcgccagac tgcgcataca ccagcgccat accaaacagc
     2341 aggaaagaag acgctgcggc agaaaggatg gtgtatttga tactggcttc cagcgaacgt
     2401 ttctggcgga aagcgtaacc gaccaggcca aacagcggca aagagatcag ttcaataccg
     2461 aggaacagag acgccagatg gtttgcattc gccagcagga tcccgcccag cgcggcaatt
     2521 aacaccaaca ggtagaactc atccttgttg tcgttatagc cttcaagcca cgggtaggcg
     2581 aaagtacagg tggcgaggct cgccaacaat accagcccgg tgtaaagcat ggcgaaacca
     2641 tcaacgcgca tcagcggcgt aacgtccata gcgcccgcct ggccaacaaa ccagagcgaa
     2701 accagcgccg cgttaagccc aataaccgag agcgtagcgt tgaggaaatg attgcgtcgc
     2761 cacgcaatgg agagcatcac aaccaccacc gttaagccga cgatcagcaa cggtagcagt
     2821 gcgatcaggt tttgtggagt tattgtcatg gcgatttacg gccttgtagt agtaacggaa
     2881 ttaacaaacc actgctggat attgccaatc gcggagtgcg aggtatccag aatcggctgc
     2941 ggatagaagc ccagcagtac cagcagcacc accagcaaca ggatcataaa cagctcacgc
     3001 agcgacatcc ctggcagttc ctggctggca atctggcttt tcgctttacc gaagtaagcg
     3061 cgatgcaaca tcgccagcga ataaacagat gcaaagacca gcccaaaggt agagataacg
     3121 gtaatcaccg ggacaacctg gaagctgccg aacagaatca taaattcgcc gacgaagtta
     3181 ccggtgccag gcatcccaag cgttgccacc gcaaagaaca gcgacaatgc tggcagccat
     3241 ttcatcttgc tccacagacc gcccatcatg cgcatgtcgc gggtatggat acgttcataa
     3301 agctgaccac aaagaataaa cagacccgcc gccgacaaac cgtgcgcaat catctggatt
     3361 accgcgccct ggtaggccaa ctggctgccg gtgtagatag caatcagcac gaagcccatg
     3421 tgggaaaccg aggtgtaggc gatcagacgt ttgatatcgg tctgggcgaa ggccatccac
     3481 gcaccgtaga agatgccgat aacacccagc cacatagcaa ttggcgcgaa ctctgccgac
     3541 gcgttcggga acagcggcag ggagaaacgc agcaaaccgt aagcggcagt tttcagcaag
     3601 atccccgcga gatcaacgga accggcggtc ggagcctggg agtgcgcatc cggcagccag
     3661 ccatgcagcg gaaccaccgg cattttgact gcgaaggcga tgaagaagcc cagcattaac
     3721 aggtattcca caccactgga cattggcgta ttcagcagct cttcatagtt gaaggtccag
     3781 acgccggtcg cattgtagtg aacaaaaacc agcgccagga tggcaatcaa catcaccagg
     3841 ccactcgcct gggtgtaaat gaagaacttg gttgccgccg tgatacgcgt tttaccgtca
     3901 gaggctttat gcccccacag tgcgatcagg aagtacatcg gcaccagcat catttcccag
     3961 aagaagaaga acaggaacat gtcgatggca aggaacacgc cgataacgcc gcccaggatc
     4021 cacatcaggt tgaggtggaa gaagccctga tatttttcga tctctttcca cgaacagagt
     4081 accgccagca caccgagcag accggtcagc acgaccatca gcagcgacag accgtcaatg
     4141 gcgagatgaa tagagatacc aaaacgcggg atccacggca tgtcgaattc agactgccac
     4201 tgcggaattc cggcggattg cgtcagtgaa taaccgccct gcaaccacag ttgcagcgac
     4261 agcgccagcg tcaatcccat ggtgatcagc gcgatccagc gtggcacctt gacgtcaaag
     4321 tgttcggtct gccagcacag gaagccgcca ataaagggaa ttaatattag ccagggtagt
     4381 aacatggcga tctttattcc ttgtaaaagt cccgtcagga ccggattttc aacgaattct
     4441 cacgacagaa aattctcttc ggactggggg tagtgtcgga tgcgccgcga aggcagctta
     4501 tccgacctac gttcttatat gcccaggagc aatcccacaa tccttaactc aacgcagtac
     4561 catcaacagt gccagcacca cgaccgcacc gatgctcatg gatgccacat accagcgcag
     4621 ataaccgttc tcgcttaaca gcaggccttt acctgcaaag cgggaaagaa ccgccggaat
     4681 gttcatcatt gagttcagcg gatcgcgttt cagcaaccag gcaataccca gaaacggctt
     4741 gacgaacact ttgtcgtaca gccagtcaaa gccccaggcg ttgtaccacc aggtgcccag
     4801 cagacggccc ggcgcactgt tggcaatgga ggtcaccaga gtacgtttac cctgccacag
     4861 ccaggctgcc agcagaatgc cgaccaccgc gaccacgcca gaggtaattt ccagggtcaa
     4921 catgctgccg tgcgccagtt ccgtcgtttg cggaagcacg ctctgcagcg gcggtacaat
     4981 cagtgcgcca acgaaggtgg aaaggatcag cagcacaatc agcggcaggc tgtgagttac
     5041 ccctttcacg gcgtgagcgt gaatttgttc ttttccgtgg aagacgatga aaatcatacg
     5101 gaaggtgtag agcgaggtca taaacgcacc gaccagacct gccaccatca gattgatatg
     5161 accattcgcc atcgcacccg cgaggatctc atccttactg aagaagcccg cagtgaccag
     5221 cggtagtgcc gacagtgctg cgccgcccac caggaagcag aaataaacca gcggaataga
     5281 tttacgcaga ccgcccatct tgaagatgtt ctgttcgtga tggcaggcca gaatgacgga
     5341 accggatgcc aggaacagca gcgctttaaa gaacgcgtga gtcatcaagt ggaaaatcgc
     5401 cgcatcccat gcctgcacgc caagcgcgag gaacatgtag ccaatctggc tcatggtaga
     5461 gtaagcgaga acacgtttga tgtcggtctg taccagcgcg gcaaaaccgg ccagcagcag
     5521 cgtaaccgcc ccgacaatgc ccaccagatg cagaatttcc ggcgtcatca ggaacaggcc
     5581 gtgggtacgg gcgatcaggt agacacccgc ggttaccatg gttgcggcgt ggatcagcgc
     5641 ggagacaggc gtcgggcccg ccatcgcgtc ggcaagccat gtctgcaacg gcaactgcgc
     5701 agatttaccg accgcaccgc ccagcagcat cagcgtcgcc cacatcagca tgttattgcc
     5761 gtcagcaaag tgcgctggtg ccagttccac catttcgcgg aagttcaggg tgcccagttc
     5821 gttgtaaaga atgaacagcg cgaaagcgag gaacacgtca cccacacggg tcacgacgaa
     5881 cgctttcatt gccgctgcgc cattcttcgg atcggtgtaa tagaacccga tcagcagata
     5941 ggagcacagg cccacgcctt cccagccgag gtacatcagc agcaggttat cggcgagaac
     6001 cagaactacc atgctggcga tgaacaggtt ggtgtaggcg aagaagcgag agtagccctc
     6061 ttcaccgcgc atgtaccagg aggcgtacat gtgaataagg aaacccacac cagtgaccac
     6121 cgagagcatg gtcagcgaca ggccgtccag caccaggtta aaaccgatgt taaagtcgcc
     6181 taccgacatc cacgtccaca gcggctggct gtatgcctgc tcgccgttag cgaagaaatc
     6241 aacgccgata aaggcggtta ccagcgccgc caggcccaca gagccaacgc cgacgatcgc
     6301 cgaaacgttt tcagaccagc gcccacggga gaacgccagc aggacgaagc caatcaatgg
     6361 caaaataatg gttaaggcaa gcatgttcat ccgcgcatct cacttactga atcgatgttc
     6421 aggttctggc gacgacggtg aagttgcagc agcagcgcaa ggccgatact cgcttctgcc
     6481 gccgcgaggc tgatggcgag aatgtacatc acctgaccgt cggtctgacc ccagtagctt
     6541 ccggcgacca caaaggccag cgcggaggcg ttaatcatga tttccagacc aatcagcata
     6601 aacagcagat tgcgacggat aaccagaccg gttaagccaa gaacgaataa gattgccgag
     6661 aggatcagtc catgttgtaa ggggatcatg cgtgctcctc cgtttttctt ttcgcgctgt
     6721 cgtctttacg attgctcagc acttcacccg cacgctcttc acgaccgacg tggaaggcca
     6781 caaccagacc cgcgagcagc agcatagaag ccagttccac cgccagtacg taaggcccga
     6841 acagcgtaat acccactgct ttagcactga ttggcgtacc gtcgataccc tgatcattaa
     6901 caccaaggat ggcgtaaaca atcaccacca gcatgatggc cgacaaaatt gccggaccaa
     6961 tccacacctg cggtttcagc cactggcgtt cctgttcgat ttctgaaccg cccaggttga
     7021 gcatcatcac cacgaacacg aacagcacca taatggcacc cgcgtagacg ataatttcca
     7081 gcgcaccggc gaagtaagcg cccagtgaga agaacacccc ggaaatcgcc agcagcgaaa
     7141 taatcaggta cagcagtgcg tgtaccggat tggtatgggt gatcactcgc aaggtcgcaa
     7201 gtatggctat caggccacag atataaaaag cgaactccat tgcccctctc cttacggtaa
     7261 caggctcttg acgtcgatag gcttggcttc gttctctgct tcgcccttat ctttgccgtc
     7321 gattgccata cctgccatcc ggtagaagtt atattccggg tatttgcccg gaccggagat
     7381 cagcagatcc tctttttcgt aaaccagatc ctggcgcttg tattccccca tttcgaaatc
     7441 cggggttaac tgaatcgccg tggtcggaca ggcttcttcg cacagaccac agaaaatgca
     7501 gcgtgagaag ttgatgcgga aaaattccgg gtaccagcga ccgtctttgg tttctgcttt
     7561 ttgcagcgag atacagccga ccgggcaggc taccgcgcag aggttacagg ctacgcaacg
     7621 ctcttcgccg tccgggtcgc gggtcagaac gatacgacca cgataacggg gcggcagata
     7681 gaccggctct tccgggtaca ttcgcgtttc gcgtttggcg aacgcgtgca ggccgatcat
     7741 ccagatacta cgaacctggg tgccgaaacc tactaacaat tcttttaagg tcatggtctt
     7801 attgcccctt attgcgcctg ccagagaatg acagccgccg ttaccagcaa gttgatcagc
     7861 gtcagcggca ggcagatttt ccagccgaag gacattacct ggtcataacg cggacgcggt
     7921 aacgacgcac gaatcaaaat gaacatcatc ataaagaacg cggttttcaa cgcgaaccag
     7981 atgaatggcg gtaacaacgg gccttgccag ccaccgaaga acagcgtcac catcaatgca
     8041 gagatggtca caatcccgat gtactcaccc acgaagaaca gaccgaactt cataccggaa
     8101 tattcaatgt ggtaaccatc cgccagttcc tgctcggctt ccggctggtc aaacgggtga
     8161 cggtgacata ccgccacgcc cgcgatggca aaggtaataa aaccaaagaa ttgcgggata
     8221 acgttccaca catgcgcctg gctgttgacg atgtcggtca tgttgaatga accggcctgc
     8281 gccaccacgc ccatcaggga aagcccgagg aacacttcgt agctcagggt ctgcgcagaa
     8341 gcacgcatcg cacccagcaa cgagtatttg ttgttacttg accagcccgc aaacagcacc
     8401 gcgtaaaccg ccagacctgc catcatcagg aagaacaaaa tcccgatgtt caggtcggca
     8461 accacccaac ccggactgac tggcacaatc gcaaaggcca gcagcagcga ggtaaaggca
     8521 atcatcggtg ccagggtaaa gatgacgcga tccgagaatt tcgggatcca gtcttcttta
     8581 aagaacattt tgatcatgtc cgcaaccagc tggagcgaac cgccccagcc aacacggtta
     8641 ggtccataac ggttctggaa cagacccagc aggcgacgtt cgccaaagct catgaatgcc
     8701 ccacaggtga caaccaccag caggatcacc accgctttga ggatggtcag caggatctca
     8761 atcagttccg gtgatatcca actcattgtt gtgcctcctt gagatcctca agatgcgcgc
     8821 cagccagcac cggagcaatg ccggacatac ccatcggcaa gcccacctgc cctgccgtca
     8881 gtccttcggc gatttcaacc ggcagcgtga ccgtgttgcc atcgtaacta aaggagacgc
     8941 gtgtacctgc gttcacaccc aacttcgcgg catccgctgg gttgagtttg atgtacggct
     9001 gcggcatacg gctctggaag accggagcac gctgtgacaa ttcatcgctg ccaaacaggt
     9061 ggtaatacgg cgcaatacgc catttcccgt cctgcggctg gaagcgtgcc ggtacgctgg
     9121 tgaagtaatc cagaccattc tcgctggttt caaacagacg cacgcccgga tcgccaaagc
     9181 gcagtttgcc gcccacttcg tcctggaatt tgttccacgc ctgcggggag ttccagcccg
     9241 gtgcccaggc aaacggcact tgcgaacggt gcgcggtcgg ctggttgtta ccttccatcg
     9301 agaaggtgaa catggtgtca atatcctgcg gctgacgcgg ctcatgaacg ctgatattgg
     9361 cgcgcatggc ggtacgaccg ctgtaacggt gcggttcacg ggccagtttc tgcccacgaa
     9421 tacggaatgt cgcatccggc gcagcatctt tgatacctgc cagttccggg attttcgcca
     9481 caacagcgtc aatcacatgg tcgagctgcg tccagtccac ttcacggctc agcagggtgc
     9541 tgtgcagcga gtgcagccag cgccagcttt ccagcatgac agttttgctg tcgtaataag
     9601 ccggatcgta aacctggaag aaacgttggg cgcgaccttc attattaatt accgtaccgt
     9661 cgctttcagc aaagctggcg gcggaaagta ccagatgggc gttttccatg atcgctgtac
     9721 gctgatggtc aaccaccatc accagcggtg ctttagccag cgcagcattc acgcgggtag
     9781 cagaagcgtg acgatgcaga tcgttttcca acaccaccac cgcgtcggcg cgtccggttt
     9841 ccagttcggt taacgcttct tcaagcgaac cgccacccat aatgcccagc cccatgctgt
     9901 tgacggaacg ggcaatcatg gtgataccga cgtcagcgcc gcgacctttc agggctttcg
     9961 cgacgttagc cgccgcctga atcacctcta agctaccggc gttcgtcccg gagataatca
    10021 acggtttctt cgcacctgcc agtgcctgca cgatgacgtc gattttgctt tgcagctcag
    10081 gttcgatacc gtcaaccgct ggtgcagagt tatccagcgc atgggcgatg gcaaaaccta
    10141 aacgcgcctg atcttcaacc ggtgcgcggt aagtccacgc cgcgatatca tccagacggg
    10201 tgtcatcaac gttggtaaca aacagcggat gcttcgcacg ttgaccgatg ttaaggattg
    10261 ccgcaatctg ccagtcagcc actttctgtg ctgccgccat ttcgcgcgct ttacctttca
    10321 ccgcctgacg cactgccagc gcgacgcgcg cgccggtctg ggtaacgtct tcacccagca
    10381 ccagtaccgc atcgtaagat tcgatttcgc gcagagccgg agtataaatg ccgccttcac
    10441 gcagcacttt cagcgccagt tgcagacgtt cctgctcacc gtgagcgata ccggtgtaga
    10501 agttttcttc gcccaccagt tcacgcagtg caaagttgct ttccacgctg gcacgcggag
    10561 aaccaatacc gatcactttc ttcgactgac gcagaatatc tgccgcgccc tgcattgcct
    10621 gttcggcgtt gagggtaatg aaatcatcgc cacgacgctg tactggctga cgcggacgat
    10681 ccttcaggtt gacgtaaccg taaccgaaac gaccacggtc gcagaggaag tagtggttta
    10741 ccgtaccgtt gtaacggttt tcgatacgac gcagttcgcc gtaacgttca ccggggctga
    10801 tgttacagcc gatggaacat tgctggcaga tgctcggcgc aaactgcata tcccatttac
    10861 ggttgtaacg ctcggagtgc gttttgtcgg taaatacgcc ggtcgggcaa atttcgacca
    10921 ggttaccgga aaattcgctt tccagcgtgc cgtcttccgg gcgaccgaag tagacgttgt
    10981 cgtgcgcgcc ataaacgccc agatctgtac cgtcagcgta atctttgtag taacgcacac
    11041 agcggtagca ggcgatgcag cggttcattt cgtgagatat gaatggcccc aaatcctgat
    11101 tacggtgggt acgtttggtg aaacggtagc gacggaagct gtgtccggtc atcacggtca
    11161 tatcctgaag atggcagtta ccgccttctt cacataccgg acagtcgtgc gggtggttgg
    11221 tcattaacca ctcgaccaca ctttcacgga actgtttcgc ttcttcgtcg tcaatggaaa
    11281 taaaggtgcc atcggaagcc ggtgtcatac aggacatcac caggcgacca cgcgtgtctt
    11341 ccgcgttttg gtattgcttc accgcacact ggcggcaagc accgacgctt cccagcgccg
    11401 gatgccagca aaagtaagga atatcaaggc ccagagacag acaagcttcc agcaggttgt
    11461 ccgctccgtt gacctcgtat tctttgccgt ctacatgaat tgtagccatt agcatgcttc
    11521 cagttttctc ggtcagggac cgagcgttaa tcgaaattcg gttaccagcg ctctttcagc
    11581 aggttcggct gaatcccatt aatcaaatgg gtattgctga acggctgttt gattcccgcc
    11641 tcaaattctt cgcggaaata tttgatggcg ctctgtaacg gctccactgc accaggtgcg
    11701 tgggcacaga aagttttacc cgggcctaag aatcgacaca gttgctcaag tgtttcaata
    11761 tcgcccggct ggccttcgcc acgctccagc gcacgcagaa ttttcacgct ccacggcaga
    11821 ccgtcgcggc acggcgtaca ccagccgcag gactcacggg cgaaaaactc ttccaggtta
    11881 cgcaccagcg acaccatgtt gatctcatgg tcaaccgcca tcgccagcgc cgtacccaga
    11941 cggctgcccg ctttaccgat actttcgaat tccatcggca gatcaaggtg cgcttcggtc
    12001 aggaagtcag tccccgcgcc gcctggctgc caggctttaa atttcagacc atcacgcata
    12061 ccaccggcgt aatcttcgag gatctcgcgt gcggtggtgc cgaacggcag ttcccacagt
    12121 cccggatttt tcacccgacc ggagaagccc atcagcttgg tgccagcatc tttacttttc
    12181 gagatgttct gataccactc cacgccgtta gcgaggatcg ccggaacgtt acacagggtt
    12241 tcgacgttgt tgacacaggt cggtttgccc catacgccgg aggttgccgg gaaggggggt
    12301 ttcgagcgtg ggttagcacg acgcccttct agggagttga ttaacgctgt ttcttccccg
    12361 cagatgtagc gccctgcccc ggtatggacg aacagttcga agtcgaaacc tgttcccata
    12421 atgtttttgc caagcaagcc cgcttcagtg gcttcggcaa tggcgcggcg cagatttact
    12481 gccgcttcga tatattcgcc acgcaggaag atgtagccac ggtaagcttt cagcgcaaac
    12541 gcggagatga gcataccttc caccagcagg tgcggcagtt gctccatcaa caggcggtct
    12601 ttataggtgc ccggctccat ttcatcggcg ttacacagca ggtaacggat gttcatggat
    12661 tcgtctttcg gcatcaggct ccacttcagg ccagtagaaa agcccgctcc gccgcgccct
    12721 ttcagaccag cgtcttttac ctgattaacg atttcgtccg gagacagccc ggtcagcgcc
    12781 ttacgcgccc cttcgtaacc gtttttgctg cggtattcgt ccagccacac tggctgtttg
    12841 tcatcgcgca gacgccaggt cagcggatgc gtttcgggag tacggataat gtttttcatt
    12901 tataccgctc cagcagttca gggatcgctt ccggggtcag atgcgcgtga gtgtcctcat
    12961 cgatcatcat gtttggccct ttatcacagt tccccaggca gcaagttggc agcagcgtaa
    13021 agcggccatc aaatgtcgtt tgccctggtt tgatgttcag ctttttctcg agcgccgcct
    13081 gaataccctg atacccgttg atatgacaga ccacgctgtc gcaataacgg atcacatggc
    13141 gaccaaccgg ctggcggaag atctgactgt agaacgttgc cacaccttcg acgtcgcttg
    13201 ccggaatacc cagcacatcg gcgatcgcgt ggatcgcacc atccggcacc cagccacgct
    13261 gcttctgaac gattttcagc gcttcaatgg acgccgcacg cgggtcttcg tagtggtgca
    13321 tctcgtgctc aatcgcttca cgctctgccg cactcagctc aaaagcctcg gtttgtggtt
    13381 gttgattctc gtgcataatt agcggtccac atctgacata acaaaatcga tactgcccag
    13441 ataaacaatc aggtcagaca ccaggctgcc gcggatcgcc gccggaattt gctgcaaatg
    13501 cgcataactc ggcgtgcgga tacgagtgcg ataactcatg gtgctaccgt cgctggtcag
    13561 gtagtaactg ttgatcccct tggtcgcctc aatcatctgg aaagattcat tggcaggcat
    13621 caccggcccc cacgacactt gcaggaagtg ggtgatcagg gtttcgatat gttgcagcgt
    13681 gcgctctttc ggcggcggcg tggtcagcgg gtgatccgct ttgaacgggc cttccggcat
    13741 gttgttgagg cactgctcaa gaatgcgcag actctggcgc agctcttcca ctttcagcat
    13801 tacgcgggtg tagcagtcag aaacaccacc accaaccggg atttcaaagt cgaagttttc
    13861 atagccagaa taaggacgcg ccttacgcac gtcgaagtcg atcccggtag cacgcaggcc
    13921 cgcgccagtg gtgccccact ccagcgcctc tttcgcgcca taggcggcaa cgccctggga
    13981 acgacctttc agaatggtat tttgcagcgc cgctttctcg taagacgcca gacgtttcgg
    14041 catccagtcg aggaactcac gcagcaggcg atcccagccg cgcggcaggt cgtgcgctac
    14101 gccgccaata cggaaccacg ccgggtgcat acggaaaccc gtgatcgctt ccaccagatc
    14161 gtaaattttc tgacgatcgg taaaggcgaa gaacacgggc gtcattgcgc cgacgtcctg
    14221 aataaaggtc gagatgtaca gcaggtggct gttgatacgg aacagttcgg agagcataac
    14281 gcgaatgacg ttaacgcgat ccggcacggt gatcccggcc agtttctcta ccgccagcac
    14341 gtaaggcatt tcgttaacgc agccgccgag gtattcaata cggtcggtat acggaatgta
    14401 gctgtgccag gactggcgct cgcccatttt ctcagcacca cggtggtggt agccgatgtc
    14461 tggtacgcag tcgacaatct cttcgccgtc gagttgtaaa acgatacgga aagccccgtg
    14521 cgccgacggg tggttcggac cgaggttgag gaacatgaag tcctcgtttt cggtgccgcg
    14581 cttcatcccc cactcttccg gtttgaaggt cagggcttcc atctccagat cctgtttggc
    14641 tttggtcagc tcaaacggcg agaattcggt agcgcgcgcc gggtaatctt tacgcagcgg
    14701 gtgacctttc caggtttgcg gcatcatgat gcggcgcagg ttcgggtgac cgtcgaaagt
    14761 aatgccaaac agatcccagg tttcacgctc ataccagtta gcgttcggga acagtttggt
    14821 gaaggtcggt acgtgcaggt cgttttctgc cagcgccacc ttcagcatga tgtcgcggtt
    14881 acgatcgata gaaatcagat ggtagaaaac ggaaaaatcc gcggcaggta acccttcgcg
    14941 gtgtgtgcgc agacgttcgt ccatgccgtg taagtcaaac agcatgacgt aaggtttcgg
    15001 cagtttcttt aagaaatcgc caacttccag taattgttca cgcttgatcc acacaacggg
    15061 aaccccggtg cgagtcgcct gaacagtaaa ggcatccggc ccaaaacggt tgcgcagttc
    15121 gccaattacc ggatcatcaa gatgatcgcg ggtctgccag gcgggttctt gcgcggttaa
    15181 gtcggtcata ttgttcacca ttgcaaatgg tccgtggtga ctgtcggcgc agtaagcttc
    15241 gcgaaataga tttaggaata agcgaagaaa aatccctttg ccgacaggcg caaattaaat
    15301 ctcgtcaggt gtacgcaggt tggttacggc aatgcgttca ccgcgcttgc gttcgcgctc
    15361 tgattgcata ttggcgcgat aaacgccctg atcgccaacc acccaggaga gcggacgacg
    15421 ttctttgccg atagattcct gcaacagcat cagtgcctgc atgtacgctt caggacgcgg
    15481 cgggcagccc gggatataca catcaaccgg gatgaattta tcgacgccct gcacaacgga
    15541 ataaatatcg tacataccac cagagttggc acaggcaccc attgagataa cccattttgg
    15601 ttccagcatc tggtcataca gacgctgaat aaccggtgcc attttggtaa agcaggttcc
    15661 tgcaaccacc atcaggtcag cctgacgcgg cgaagcacgc aatacttctg cgccaaaacg
    15721 cgccacgtca tgcaccgcgg taaacgaagt caccatctca acgtaacagc aggaaagacc
    15781 gaagttatac ggccaaattg agtttttacg accccagtta accatgtcat tgagcttgcc
    15841 cataaacacg tttttgttaa cttcttgctc cagagggtcg gttacgatct cctgcttttg
    15901 cagggggtaa cggtcgttct caccgttggg atctatgcgg gtgagcgtat aatccatctt
    15961 aatgcctcgc ggttagcgtt gacgattagc gatactgttc gtttccgggt tcatacgctc
    16021 gcggcgtgaa cgcgcgggcg tccagtccag cgcgccaata cgcaccagat aaaccagacc
    16081 tgccagtaac acaaaaataa aaattgcagc ttccacaaag cctacccagc cgctttcgcg
    16141 gatagaggtt gaccatgcga acagatacag cgcttcaacg tcgaagataa cgaagaacat
    16201 ggccaccaga taaaacttgg cggacaggcg taagcgggcg gagccgaccg agtcgatacc
    16261 ggattcaaac ggcacgtttt tcgacctcgc gcgtgcgcga ccgcctaaaa accaaccacc
    16321 taccagcatt aggcaacaca ggccaatggc aacgataaga aagatagcga atgcccagtg
    16381 atgagcgatg acttcagtgg atgttgacat actcattgct tactcatcaa aagtagcgcc
    16441 agattcactg ctcttcacgg cagatggacg ccacatcgat tcatggggag gaataaaaaa
    16501 aaccttacaa tcactgtaga aattctttta tacagctaat tgatgtggtc ttttactcct
    16561 ttctataacc ttttgtcaac tttaacaaaa gtttcttcac attagtttac ataatatcaa
    16621 caccattagc atttaatgcc ctttcacccc agctccttga cgactccagg ataattagat
    16681 gttgttgaat cgtgtccgtt gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa
    16741 tttgtgtcga ttcctccccc caaaagagag tattttcttg atctgtgaca cgcttttgtc
    16801 attccataac aaaaacgcag caacaaattt acgtattttt taacatcatt gtagcaggtg
    16861 atttttttca ggcgattatt tgtgcgttcg ggacgtgaat ctctggtgat taaaaaatga
    16921 acagttttgt acgttctgca ctatgcgagg aaggattttt actaaaaaaa agccgctggg
    16981 gtttaaaaca cccccagcgg ctcgtttttt acactattgt ctcaggaatt atctatcgtc
    17041 cgtcgattac tcgatatccc tttcaatcaa caatgaatca tccccttccg gagcagacat
    17101 tgggctgtat tgccacggat tatggtagct ctccatcgcc tgatagatca cctgggccag
    17161 ctcattatta ctggacgggt catagcacag caaatattcg gtatcaggta gcggcggtaa
    17221 gccatctact ccactcagca cacgcaggtc cgggctcatc atctcaaccg gccttgccgt
    17281 cacgccaaga ccggctttca ctgccgcacg aacggccgga agcgtcgagg cgacataagc
    17341 cagtcgccat ggaatatctg ctttattaag cgttgccagc accatatcgc gaaacggact
    17401 tggatcatcc agcaatacaa gagggatcgg ctcgcctttt tgcaatacgt attccgctgc
    17461 gcagtaccag tgtgttggcg aggtacgcag gttcaatgcc ttaaacgctg aaggacgatg
    17521 ggtggttacc atcaaatcca cttcctggga ttccagcatt tctgccatat aggcattacg
    17581 tttaacgcgg acatccagcg ctaatttcgg ataaaccgaa ctcacgcgat ttaacaggaa
    17641 aggtaagatc gtatcggcag attcatctga agcaccgata gttaacacgc cctgaagatt
    17701 actgaacatt aatgatgagc aggcctcatc attaaaacgc aggattttcc tggcgtaacc
    17761 aagaagttga atgccatgtt cagttaacag tttgttgcga ccgtgacgag cgaacagttc
    17821 tttcccaacg agttgttcca gacgctgcat ttgctgactt acggcggact gagtacgaca
    17881 cacagcggca gctgcggcag caaaagtgtt cagatcggca acagcaacaa atgttctcag
    17941 cagatcgagg tcgaggttaa ttatcggacg atttgcactt atcatatatt atcacttact
    18001 ggcggctcat actgagctgg ttaatgctgt gcacacacaa acaagcaatt ccatttgtaa
    18061 tgtgcctccc tggcagtttc atccggaacc cggacgaaag taaaaatgca tatgagttgc
    18121 actaaaaaag cgactcacat tgttccgtta taatgcctga agtagatcac agaatatatc
    18181 ttcagggatc gcatatctat taagttactc actcttttct atttatgaca tgcgcgtgtt
    18241 tgtataaatg taaatgtgag tccttgttcc actctcgtgc agcatcgctg gtcatacgcg
    18301 aacacgtaca aacagcaatg gtgaggcggc atcaagagca gggatccgtc atttatgcga
    18361 gcattttacc ccaaaaacct tttatttata agggccattg cgaatcatct gatgcaaagt
    18421 tatgttatgt taggcaaagt aatcttctat tattaataag cacatcaaaa cctttttgaa
    18481 tattaaataa taattaatta gcatcatcct cattcattaa ttccgcttaa caatagtttc
    18541 acaccttgcc ccattacgac acctcggaaa tcatcacgct gttaaactct ccgtaatgcg
    18601 gaaatatcat catcgcgttt cattagtgaa ttcttaaatg aggcattttc acacaattat
    18661 cttacagata aaaaaaccag acttacaatt aagaatcaga acaattcact atataacatt
    18721 gcatgtaaag catatacacc tcattatttt gtcatcatta agttattaac agcacaatcg
    18781 agccttcccc tctggcaaaa tcttattctg cagaccttca aaacaccgtc ctgggggagt
    18841 acattgttct aagctgactt ccacggcagg gagtggcgat aacagcaaaa aaggtcaaga
    18901 ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg tctgttatga catccgtggt
    18961 ccggtgctga aagaagcaaa acgcctggaa gaagaaggta acaaggtact gaaactgaac
    19021 atcggcaacc cagccccgtt cggttttgac gcgccagatg aaatcctcgt tgacgtgata
    19081 cgcaacctgc ctaccgctca agggtattgc gattccaaag gtctttactc cgcgcgtaaa
    19141 gccatcatgc agcactacca ggctcgtggc atgcgtgatg ttaccgtgga agatatttac
    19201 atcggcaatg gtgtatcgga gcttatcgtt caggcaatgc aggcattgct gaacagcggg
    19261 gacgaaatgt tggttcctgc accagattac ccactatgga ccgcggcggt ttcgctttcc
    19321 agcggtaaag cggtgcatta tctttgcgat gaatcctctg actggttccc ggacctcgat
    19381 gatattcgcg ctaaaattac gcctcgtacg cgtgggatcg ttattatcaa cccaaataac
    19441 ccaaccggcg cggtatattc caaagagctt ttaatggaga ttgtggagat tgcacgtcag
    19501 cataatctca ttatcttcgc cgatgaaatt tatgacaaaa ttctttacga cgacgctgag
    19561 catcactcaa ttgcgccgct ggcacctgac ctgctgacca ttacctttaa cggactgtcg
    19621 aaaacgtacc gcgttgcagg cttccgtcag gggtggatgg tgttgaacgg gccgaaaaaa
    19681 cacgccaaag gctacatcga aggtctggaa atgctggctt caatgcgcct gtgtgctaac
    19741 gttcctgcgc aacacgccat tcagaccgcg ctgggtggtt atcagagcat cagtgaattt
    19801 attacccctg gcggtcgtct ttatgagcag cgtaaccgcg cgtgggaact gatcaacgat
    19861 attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt atatgttccc gaaaatcgac
    19921 gccaaacgct ttaacattca cgacgatcag aaaatggtgc tggatttcct gttgcaggaa
    19981 aaagttctgt tggtgcaagg gacggcattc aactggccgt ggccggatca cttccgcatt
    20041 gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga gcaagttcgc gcgtttcctt
    20101 tctggttatc atcagctgta atcttaattt cactgccgga gattgcatcc ggcagcgtta
    20161 tcccgccaca atgacctgat gatgtcatca tacgtaaggt cactatgaaa cagagccatt
    20221 tttttgccca tctctcccgc ctgaaactca ttaaccgctg gccgctaatg cgcaacgtgc
    20281 tgacggaaaa tgtgtccgaa cacagtttgc aggtagcgat ggtcgcccat gcgctggcag
    20341 ctatcaaaaa tcgaaaattt ggcggtaatg tcaacgccga acgtatcgct ttactggcga
    20401 tgtaccacga tgcctcagaa gtgctcaccg gcgatctccc tactccggtg aaatacttca
    20461 attcgcaaat cgctcaggag tacaaggcta ttgaaaaaat cgctcagcaa aaactggtcg
    20521 atatggttcc ggaagagctg cgggatatct ttgcgccgtt aattgacgag catgcatata
    20581 gcgatgaaga aaaatcgctg gtgaaacagg cagatgcact gtgtgcatat ctgaaatgtc
    20641 tggaagaact cgcggccgga aataatgaat tcttgctggc aaaaacgcga ctggaagcga
    20701 cgcttgaagc gcgtcgcagc caggagatgg actacttcat ggaagtattt gttcccagct
    20761 tccatctttc gctcgatgag attagccagg attcaccgct gtaagcagcc ggagtctgcg
    20821 tcgcatcagg caataagcgc cggatgcgac atcaggctct tgtcaaaacg gaaacagcat
    20881 cgggatcatc accacacaaa ccgccatcac gataatggtg aacggtaccc ccaacttcac
    20941 aaagtcactg aagctgtaat ttcccggacc taaaaccagt gtgttaacag gtgaagaaac
    21001 cggtgtcata aaggcggcgg atgctgccat cgccacgacc atcgcgaatg gataaggcga
    21061 cacccccatc gttttggcag cagccagcgc aatcggagcc atcaacaccg ccgtcgcggt
    21121 attagagata aatagcccaa taaccgccga caagacaaac aaacacccca gcatcatatg
    21181 tggcccgtaa ccaccgccaa tgtccatcag ccctttcacc gccagcgcga cacctcccgt
    21241 tttctgtaat gccacagcaa acggcatcat cccaacgatc aaaataatgc tcggccagtg
    21301 aatggattta taggcgcttt cagcatctat acagcggaat ttccccatca gcaggcaggc
    21361 gatgatagcg gcaacaggat taggaatttc atctgtcagc attaacgcca ccattagcac
    21421 cagacagaaa atggcatggg gtgcctggct gtgcgcgggc gatgcttcac tcacctcttc
    21481 cggtaagttc agcgctacga agtcgcggcc ctgtttggcc agcataccga tcagtttcca
    21541 gttacccaca accaggatga tatcgcccag cagcagaggc tcatccgcca gcgaaccttc
    21601 cagcgccacg ccattgcgct ttagccccac cacattcagt ccgtagcggg tacgaaaacc
    21661 aatttcgcgc accgatttac caatcagttc tgactcagga attaatgaaa tctctgccat
    21721 gcccacatca agggcctggt cagaaaaata ctcgccgcgc agtaccatcg gctccagcaa
    21781 ttgctcacta caaaattgcc ggagatcgac atcagccgca gacatatcaa taagcaaaac
    21841 gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg ataacgcgac gaaaacgccg
    21901 ccagcgttca acaccgatga cgttagcgcc ataacgctca cgtaatttga gatcatccag
    21961 ccgttgacca atcattggcg atccggggcg aatagccaga cgtcgcgcac gcccggtcag
    22021 tcgatattca cggataagat cgcgaaaggt tcgacgcgtc cagccttcgc gctgcggggt
    22081 ctgggtatcc cctttcagca tgaaacgcat cactaacata tacaaaatac ccagcaccag
    22141 cacgaccagg ccaacaggtg ttacgctaaa gaaactgaag ccgtgatagc cttcacgcag
    22201 caattcactg ttgactacca ggttcggcgg cgtcgccacc agcgtcatca tgccgctaat
    22261 cagcccggca aaacttaacg gcatcatcag acgtgacggc gaagtttgca tacgcatggc
    22321 aacgcttaac accacgggaa taaagatagc gacaacgccg gttgaactca taaacgcgcc
    22381 aagccccgcg acggtcagca tcaacaaaac caacattttg atttcactgt tgcccgccac
    22441 tttgaccagc catgttccca ttacggtggc aacaccggta cggaccaaac catcgccaat
    22501 aataaacaag gcggcaatca ggacaacgtt aggatcagaa aagccggaaa atacttcggg
    22561 gaccgtcagc gttccgctta atgcaaacgc gacaataaca aacaaagcga ccgcatccat
    22621 acgcactctg cccgtcgcaa acaagacgat ggcaacggcc agtaatgaaa gaacccaaat
    22681 caattcaccg ttcacaactt atccttgtta attgaggggg atgacttgat tctgccataa
    22741 aaaagccccg acgagacggg gctaaatcat gatcaggtgt ttcactgaat aataacatcg
    22801 ccatttagct gtttggtcac agtaatttgc tccagactgt ggaggaccaa atcgacctca
    22861 ttcaggcgcg gggtatctgc cggagcgtta accgcaatga catggcaacc cgccgccagg
    22921 ccagaaagca cgccagcggg agcatcttcc gccaccacac actcctgcgg cgcaagcccc
    22981 agcagctgcg cacctaacag atacgcatca ggttctggtt ttccgcgctt cactcgctca
    23041 gcggttacaa acacctttgg tgcgggaagc ccagctattt tatggcgcgc tcgcgctacc
    23101 ggcatggagc cagaagtcac aatggcccac ggaatacctg ctttattcaa atgattgagt
    23161 aaggcgattg cccccggaag cgcggtaata ccttcggttt ccgtggcctc gatgtgctcc
    23221 agacacgtaa actcggcggc aatatcagcc tcggatttgc ccgccataaa atggcgcaaa
    23281 gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct cttccggcgc taacccatga
    23341 cgtctggccc agttgctcca cgcccgttct accgcaggca gggaatccac cagcgttcca
    23401 tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct cctcaggcgt tgataatttg
    23461 attaatttcg ttggcgctca aatggtactg acgcaggcag gcatgccaca cattaagcat
    23521 gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa ccatgagtcc cagcgtcaaa
    23581 atgggtatag cgcccttcca cattaaccat aaagcggaca taaccgaggt aacgtgcttc
    23641 agtggcggcg tcaaagccga ggaaggtgac acgacgttca tcgatggatt gctggtcctg
    23701 caaattagac caggaaacat gcaacgcatg atacatctcc ataatgtcga tgatagtgcg
    23761 gcaggtttct tctttcagct cgccaaactc gcgatccaat tcacgcatct gtaatccgta
    23821 accacgctca ataattgttt gcaggcgacg gtaacgttca gcatttgccg gatcgagcat
    23881 agtcatcatc ttgtactgat tagacaaaat aagacgttgc gcgttggtca tttccattgt
    23941 tgactcctgt atcactctac tacggtgaaa aaaaagaagg ctgagtatgc cttcttttat
    24001 atgcgtaatc aggggtcaat tacaaatcat caaggaaagt tttatccagt tgtttgaagg
    24061 cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt gccttcaacg ggtgccaaca
    24121 cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa ccagttgagg atagcagggg
    24181 gtaatggcgt tacagaacgc ttgcccagcc accataatcc ctgcatgggt aaacttaagg
    24241 cgaacagcgc agtggcaact gccggcccaa gctgaccgcc cagggcaatc tgccagcaga
    24301 gagtaaatac ggcgatcggc ggcataaaac ggatcgcata acgcgtcatc ttgataacgc
    24361 gattttcgac aaagaccggg gcaaggcgtt tttccagcgg ccacgtcttt gagtaatgct
    24421 gtccccggcg aaacaagcta aaaaaattaa cagaacgatt atccggcgtt gacatgcttc
    24481 acctcaactt cacatataaa gattcaaaaa tttgtgcaaa ttcacaactc agcgggacaa
    24541 cattcaaaac attttgtctt ccatacccac tatcaggtat cctttagcag cctgaaggcc
    24601 taagtagtac atattcattg agtcgtcaaa ttcatataca ttatgccatt ggctgaaaat
    24661 tacgcaaaat ggcatagact caagatattt cttccatcat gcaaaaaaaa tttgcagtgc
    24721 atgatgttaa tcataaatgt cggtgtcatc atgcgctacg ctctatggct ccctgacgtt
    24781 tttttagcca cgtatcaatt ataggtactt ccatgtcgag taagttagta ctggttctga
    24841 actgcggtag ttcttcactg aaatttgcca tcatcgatgc agtaaatggt gaagagtacc
    24901 tttctggttt agccgaatgt ttccacctgc ccgaagcacg tatcaaatgg aaaatggacg
    24961 gcaataaaca ggaagcggct ttaggtgcag gcgccgctca cagcgaagcg ctcaacttta
    25021 tcgttaatac tattctggca caaaaaccag aactgtctgc gcagctgact gctatcggtc
    25081 accgtatcgt acacggcggc gaaaagtata ccagctccgt agtgatcgat gagtctgtta
    25141 ttcagggtat caaagatgca gcttcttttg caccgctaca caacccggct cacctgatcg
    25201 gtatcgaaga agctctgaaa tctttcccac agctgaaaga caaaaacgtt gctgtatttg
    25261 acaccgcgtt ccaccagact atgccggaag agtcttacct ctacgccctg ccttacaacc
    25321 tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg caccagccac ttctatgtaa
    25381 cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga actgaacatc atcacctgcc
    25441 acctgggcaa cggtggttcc gtttctgcta tccgcaacgg taaatgcgtt gacacctcta
    25501 tgggcctgac cccgctggaa ggtctggtca tgggtacccg ttctggtgat atcgatccgg
    25561 cgatcatctt ccacctgcac gacaccctgg gcatgagcgt tgacgcaatc aacaaactgc
    25621 tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac cagcgactgc cgctatgttg
    25681 aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat ggacgtttac tgccaccgcc
    25741 tggcgaaata catcggtgcc tacactgcgc tgatggatgg tcgtctggac gctgttgtat
    25801 tcactggtgg tatcggtgaa aatgccgcga tggttcgtga actgtctctg ggcaaactgg
    25861 gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc tgcacgtttc ggcaaatctg
    25921 gtttcatcaa caaagaaggt acccgtcctg cggtggttat cccaaccaac gaagaactgg
    25981 ttatcgcgca agacgcgagc cgcctgactg cctgatttca caccgccagc tcagctggcg
    26041 gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga ggataaaccg tgtcccgtat
    26101 tattatgctg atccctaccg gaaccagcgt cggtctgacc agcgtcagcc ttggcgtgat
    26161 ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc aaacctatcg ctcagccgcg
    26221 taccggtggc gatgcgcccg atcagactac gactatcgtg cgtgcgaact cttccaccac
    26281 gacggccgct gaaccgctga aaatgagcta cgttgaaggt ctgctttcca gcaatcagaa
    26341 agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct aacaccaaag acgctgaagt
    26401 cgttctggtt gaaggtctgg tcccgacacg taagcaccag tttgcccagt ctctgaacta
    26461 cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt atgtctcagg gcactgacac
    26521 cccggaacag ctgaaagagc gtatcgaact gacccgcaac agcttcggcg gtgccaaaaa
    26581 caccaacatc accggcgtta tcgttaacaa actgaacgca ccggttgatg aacagggtcg
    26641 tactcgcccg gatctgtccg agattttcga cgactcttcc aaagctaaag taaacaatgt
    26701 tgatccggcg aagctgcaag aatccagccc gctgccggtt ctcggcgctg tgccgtggag
    26761 ctttgacctg atcgcgactc gtgcgatcga tatggctcgc cacctgaatg cgaccatcat
    26821 caacgaaggc gacatcaata ctcgccgcgt taaatccgtc actttctgcg cacgcagcat
    26881 tccgcacatg ctggagcact tccgtgccgg ttctctgctg gtgacttccg cagaccgtcc
    26941 tgacgtgctg gtcgccgctt gcctggcagc catgaacggc gtagaaatcg gtgccctgct
    27001 gctgactggc ggctacgaaa tggacgcgcg catttctaaa ctgtgcgaac gtgctttcgc
    27061 taccggcctg ccggtattta tggtgaacac caacacctgg cagacctctc tgagcctgca
    27121 gagcttcaac caggaagttc cggttgacga tcacgaacgt atcgagaaag ttcaggaata
    27181 cgttgctaac tacatcaacg ctgactggat cgaatctctg actgccactt ctgagcgcag
    27241 ccgtcgtctg tctccgcctg cgttccgtta tcagctgact gaacttgcgc gcaaagcggg
    27301 caaacgtatc gtactgccgg aaggtgacga accgcgtacc gttaaagcag ccgctatctg
    27361 tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat ccggcagaga tcaaccgtgt
    27421 tgcagcgtct cagggtgtag aactgggtgc agggattgaa atcgttgatc cagaagtggt
    27481 tcgcgaaagc tatgttggtc gtctggtcga actgcgtaag aacaaaggca tgaccgaaac
    27541 cgttgcccgc gaacagctgg aagacaacgt ggtgctcggt acgctgatgc tggaacagga
    27601 tgaagttgat ggtctggttt ccggtgctgt tcacactacc gcaaacacca tccgtccgcc
    27661 gctgcagctg atcaaaactg caccgggcag ctccctggta tcttccgtgt tcttcatgct
    27721 gctgccggaa caggtttacg tttacggtga ctgtgcgatc aacccggatc cgaccgctga
    27781 acagctggca gaaatcgcga ttcagtccgc tgattccgct gcggccttcg gtatcgaacc
    27841 gcgcgttgct atgctctcct actccaccgg tacttctggt gcaggtagcg acgtagaaaa
    27901 ggttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct gacctgatga tcgacggtcc
    27961 gctgcagtac gacgctgcgg taatggctga cgttgcgaaa tccaaagcgc cgaactctcc
    28021 ggttgcaggt cgcgctaccg tgtttatctt cccggatctg aacaccggta acaccaccta
    28081 caaagcggta cagcgttctg ccgacctgat ctccatcggg ccgatgctgc agggtatgcg
    28141 caagccggtt aacgacctgt cccgtggcgc actggttgac gatatcgtct acaccatcgc
    28201 gctgactgcg attcagtctg cacagcagca gtaatctcgt catcatccgc agctttgcgc
    28261 tgcggatatc tgaaccggaa ataatcacta tttccggttt tttattctct taatctgcat
    28321 taatcctttc tgattatctt gcttaactgc gctgcatcaa tgaattgcgc catttcactt
    28381 tgcatactta ccactttgtt ttgtgcaagg gaatatttgc gctatgtccg caatcactga
    28441 atccaaacca acaagaagat gggcaatgcc cgatacgttg gtgattatct tttttgttgc
    28501 tattttaacc agccttgcca cctgggtagt tccggtgggg atgtttgaca gtcaggaagt
    28561 gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc gtagatccac actcatttcg
    28621 cattctgact aacgaagcag gcgaacctga gtatcaccgc gtacagctgt tcacgacggg
    28681 cgatgaacgc ccgggcctga tgaacttccc gtttgaagga ttaacctcag gatcgaaata
    28741 cgggacagct gttggcatca tcatgtttat gctggtgatt ggcggcgcgt ttggcattgt
    28801 gatgcgtaca ggaaccattg ataacggtat cctggcgctt attcgccata cccgcgggaa
    28861 tgaaattctc tttattcctg cgctgtttat tctgttttca cttggcggcg cggtatttgg
    28921 tatgggagaa gaggccgtcg cctttgccat tatcatcgca ccgctaatgg tccggctggg
    28981 ctatgacagt attaccaccg tcctggtgac ctatattgcc acgcaaatcg gttttgccag
    29041 ttcgtggatg aacccgtttt gtgtggtcgt tgctcagggg attgccggcg ttccggtgct
    29101 ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc actatgattg gcctgatctt
    29161 taccatggtg tacgcctcac gagtgaaaaa gaatcctctt ctgtcacgcg tgcatgagtc
    29221 cgaccgcttc tttcgtgaaa agcaggcgga tgttgaacaa cgtccgttta cctttggtga
    29281 ctggctggta ttgattgtcc tgaccgccgt aatggtctgg gtgatttggg gcgtgatcgt
    29341 taatgcctgg tttattccag aaattgccag ccagttcttc accatgggtc tggtgattgg
    29401 catcatcggc gttgtttccc gccttaacgg catgacggtt aataccatgg cttcatcctt
    29461 taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg gtgggtttcg ccaaagggat
    29521 tttgctgctg gtcggtaatg gtgaagcggg tgatgccagc gtgttaaata ccatcctcaa
    29581 cagcattgcc aatgccatta gcggtctgga caacgcggtc gcggcctggt ttatgttgct
    29641 cttccaggcg gtatttaatt tcttcgtgac gtccggttct ggtcaggcgg cgttaaccat
    29701 gccgttactg gcaccgcttg gcgatctggt cggtgttaac cgtcaggtta ccgtgctggc
    29761 attccagttt ggtgatggct tcagtcacat catttaccca acctcagctt cgttaatggc
    29821 aactctcggt gtgtgcaggg tggacttccg taactggctg aaggtgggcg cgaccctact
    29881 tggactgctg tttattatgt ccagcgtcgt agtgatcggc gctcagttga tgggctacca
    29941 ctaaaaatgt taagagccgc aattgcggct cttttttcat tctgccgttt cagtctctac
    30001 ggcttcattt ttggcattgc gcttcatcca caacgccagc gctttcagcg agtctggagt
    30061 gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc cagcaaactt cactgacttc
    30121 atcttcctgt agggcgaagg gaccgtgaga gacgcagctg aacaacgcgc cccagacacg
    30181 gcaattttta tcttcgaaat agaactgccc gtgctcggca aaggggacac cggcaatgcc
    30241 caactcttct tccgcttcgc gacgcgcgga ttccagcagt tgctcatcgg cctgaaccac
    30301 accgcctgcg gtcgcatcta acatgccggg taaaaagtct tttgtctcgg tacgacgctg
    30361 gaccagaatt ttgcccatgc catcatgcac gacgatgtaa gttgcacgat gacgcagaca
    30421 ctgtgcccgc atttgttccc ggctggcttg tgcaatgact tcgttctctt cattgacaat
    30481 atccacccat tcagtacttg ccaaacgacg ctgttccatt atcaggaaac cttctttttc
    30541 tggcgctctt acggcgcatt ttgagttgtg ggtaaattac ggattaatcg cgacctgcgc
    30601 aatgatactt tgatcattga gtgcgattac actaagtacg tcattatcca gcatgccata
    30661 actcgccgga ttaccgcctt tcggaatact caccgagccg gggttgaagt ggaaaatctc
    30721 cccccgctgt tctgccactg gcagatgggt atgaccgtac accagcacat cgttctggtt
    30781 taaagcaggt agattttccg ggccaaaaag atgaccatgc gtcaaaaaca gacgttgttt
    30841 ttccagtaat acctgttgcc acggcgcggt tatcgggaaa tgcagcagca tttgatccac
    30901 ttcgctgtcg cagttgccgc gcacagcgat aaccttatgt gccacttcat taagccgttc
    30961 agcgactttg gctggcgcgt aaccctccgg taaagcatta cgcgggccgt gattcaacac
    31021 gtcgccaagg atcaccagcc actgggcacc gctttgggca aacaactcca gaacacgttc
    31081 cgtcgccggt aacgacccat gaatgtccga tgcaaacatc agtttcatca ctcactcctc
    31141 gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg ctatcagcca gattgcttcg
    31201 ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca ctggaatgtc gcataatcca
    31261 ccaggcgttc tggcacctca tcgccatgta gtaccaggcg gttaatcatt agcgccagat
    31321 cagtatcagc aatgcaccat tcaccaaata aattcggctg acccagtgct aacaaatgtt
    31381 ctgccatcgc gacactggct tttccctcgg ccgttagtgg cgcttttttc gcccccgcaa
    31441 agacaacatc cgtcggacgc tcttcgcgga tgggcatcag atcgctgcgc agccaggcct
    31501 gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg ataaatacgt tcccaggtcg
    31561 gtggcgcaaa tcgatcttcc agatactccg caatggcaga agattcactc aactcaaaat
    31621 catcgatttg taataacggc acacggcgtg tctgaccgta accttgccac gtcggctgca
    31681 aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa cgacaggcct ttttcctgca
    31741 acgccaccca agcggataac acataagggg agaaaaagtg ggcatctgac caaagcgtga
    31801 tagcgggttt actcattatg tcctcggcaa taaaggaggc tggctatcaa actatagagc
    31861 ctttttgtca ctgtcacgtg acgaatactc acgtaagtcg ccgcgctttc tatacttgtg
    31921 gtgttcagat gattaaacaa ccggagctgc aatgatcgat ctctatttcg ccccgacacc
    31981 caatggtcac aaaattacgc tgtttctcga agaagcagag cttgattatc gcttgattaa
    32041 ggtagacctg gggaaaggcg gtcagtttcg tccggaattt ttgcgcattt cgcccaacaa
    32101 caaaattccg gcaattgttg atcattctcc tgccgatggc ggcgaaccgc taagcctctt
    32161 tgaatctggt gccattttgt tgtatctggc tgagaaaacg gggctctttt tgagtcatga
    32221 aacgcgtgaa cgcgccgcca cattacagtg gttattctgg caggtaggcg aactggggcc
    32281 gatgcttggg caaaatcatc attttaatca cgcagccccc caaaccattc cttacgctat
    32341 tgaacgttat caggttgaaa ctcagcgtct ttaccatgta ctaaacaagc ggctggaaaa
    32401 ctcgccctgg ctgggaggcg agaactacag cattgcggat attgcctgct ggccgtgggt
    32461 taatgcctgg actcgccagc gaattgacct cgcaatgtat ccggcagtca agaactggca
    32521 tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg ctaaaagcac aactcggtga
    32581 tgagcgttcg gatagttaac agaaacaggt tctcgtgtat tatttcatcc taagtaaaac
    32641 aacggagaac ctgcaatggc acaacctgcc gctattattc gtataaagaa ccttcgttta
    32701 cgtacgttta tcggaattaa ggaagaagaa attaacaacc gtcaggatat tgttatcaat
    32761 gtgacgatcc actaccccgc cgataaagcg cgcactagcg aagatatcaa cgatgcgctg
    32821 aattatcgca ccgtaacgaa aaacattatt cagcatgtag agaataaccg tttctctttg
    32881 ttggaaaaat taactcagga tgtgctcgat atcgcacgtg aacatcactg ggtgacgtat
    32941 gctgaagtgg agatcgataa actgcacgcg ctgcgctacg ccgattcggt atccatgacc
    33001 ttaagctggc agcgttaacc gccatatcgg gaggctgcat gaacatagtg atcaccggag
    33061 ggacgggatt aattggtcgc catttgattc cgcgtttgct ggagctgggc catcaaatca
    33121 cggtagtgac gcgtaaccca cagaaagcca gttccgttct cggccctcgg gtgacgctat
    33181 ggcaagggct agccgatcaa agcaacctca acggcgttga tgcggtaatc aacctggccg
    33241 gagaaccgat tgctgataaa cgctggactc acgagcaaaa agagcgtctc tgccaaagcc
    33301 gctggaatat cacgcaaaaa ctggtcgatt tgattaatgc cagcgacacg ccgccgtcgg
    33361 tactcatttc cggctcggca acgggctatt atggcgactt aggtgaagtg gtggttaccg
    33421 aagaggaacc gccgcataac gaatttactc ataaactctg cgcccgctgg gaagagattg
    33481 cctgccgggc gcaaagtgac aaaacgcgag tgtgcctgct gcgaaccggc gtggtactgg
    33541 caccggacgg cggtattctc ggtaaaatgc tgccaccgtt tcgtcttggc ctgggcgggc
    33601 cgattgactc tggtcggcag tatctggcct ggattcatat tgatgatatg gtcaacggta
    33661 ttctctggct gctggataac gagctgcgcg ggccatttaa tatggtttcg ccctacccgg
    33721 tacgcaatga acaatttgcc catgcgctcg gtcacgcgct gcatcgcccg gccattttgc
    33781 gcgtccccgc gaccgccatt cgactgttaa tgggcgaatc ttcagtactg gtattaggtg
    33841 gacaacgcgc gctgcctaaa cggctggaag aagcgggttt tgcgtttcgc tggtacgatt
    33901 tagaagaggc gctggcggat gtcgttcgct gatgtggttt acagcaaaca tccgccagtt
    33961 aactcccggt gttacaggat tagtggcttt gcgcgataag atcgtctggt gaaagtcggg
    34021 tcaccatcat aactaactct ctgtctaaac ctctatccag catctcctga gcaatacgca
    34081 gggcttcttc gtgtttgccc tgcattgcgc cttcttcacg taatctgtca gcaatggtca
    34141 tcagtttctc cttttcttgt ggtgcgcgtt ccgctatctc accaataaat gcacgaaaac
    34201 gctgggcatc ccctgtttgt aatacgtaat taaacagggc ttttagctgt ctgtcattag
    34261 tgttccctgt aactagcagc gaaacaattt ggtcgactaa tcccaacaga tcgcgctgac
    34321 gaatatgttt ctgaattaac tccaacagcg ccattttgcg gtgttgcata atctcgtcat
    34381 ccggcaccac ggtaatatcc accaacggaa acgctgatga atatattttg cgggctatag
    34441 caggctcggc aaattcatca agccagcaga gtgaataagg ataaggactt ctgcaaccat
    34501 gataaaacag catcgggatc accaatggaa gctctttata gcccg
//
LOCUS       DAJVOY010000024        34161 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVOY010000024 DAJVOY010000000
VERSION     DAJVOY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.024"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(312..524)
                     /gene="rpmE"
                     /locus_tag="OQL90_001013"
     CDS             complement(312..524)
                     /gene="rpmE"
                     /locus_tag="OQL90_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1367868.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            727..2925
                     /gene="priA"
                     /locus_tag="OQL90_001014"
     CDS             727..2925
                     /gene="priA"
                     /locus_tag="OQL90_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1367869.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3081..4106
                     /gene="cytR"
                     /locus_tag="OQL90_001015"
     CDS             3081..4106
                     /gene="cytR"
                     /locus_tag="OQL90_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1367870.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4198..5157
                     /gene="ftsN"
                     /locus_tag="OQL90_001016"
     CDS             4198..5157
                     /gene="ftsN"
                     /locus_tag="OQL90_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1367871.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHNKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5250..5780
                     /gene="hslV"
                     /locus_tag="OQL90_001017"
     CDS             5250..5780
                     /gene="hslV"
                     /locus_tag="OQL90_001017"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1367872.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5790..7121
                     /gene="hslU"
                     /locus_tag="OQL90_001018"
     CDS             5790..7121
                     /gene="hslU"
                     /locus_tag="OQL90_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1367873.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7188..8114
                     /gene="menA"
                     /locus_tag="OQL90_001019"
     CDS             7188..8114
                     /gene="menA"
                     /locus_tag="OQL90_001019"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1367874.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8207..8692
                     /gene="rraA"
                     /locus_tag="OQL90_001020"
     CDS             8207..8692
                     /gene="rraA"
                     /locus_tag="OQL90_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1367875.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="OQL90_001021"
     CDS             complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="OQL90_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1367876.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9448..10293
                     /gene="glpF"
                     /locus_tag="OQL90_001022"
     CDS             9448..10293
                     /gene="glpF"
                     /locus_tag="OQL90_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCS1367877.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10316..11824
                     /gene="glpK"
                     /locus_tag="OQL90_001023"
     CDS             10316..11824
                     /gene="glpK"
                     /locus_tag="OQL90_001023"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1367878.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRSE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11959..12969
                     /gene="glpX"
                     /locus_tag="OQL90_001024"
     CDS             11959..12969
                     /gene="glpX"
                     /locus_tag="OQL90_001024"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1367879.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13066..13812
                     /gene="fpr"
                     /locus_tag="OQL90_001025"
     CDS             13066..13812
                     /gene="fpr"
                     /locus_tag="OQL90_001025"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1367880.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="OQL90_001026"
     CDS             complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="OQL90_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1367881.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14272..14571)
                     /locus_tag="OQL90_001027"
     CDS             complement(14272..14571)
                     /locus_tag="OQL90_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1367882.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSVDEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14783..15223)
                     /locus_tag="OQL90_001028"
     CDS             complement(14783..15223)
                     /locus_tag="OQL90_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1367883.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15324..15923
                     /locus_tag="OQL90_001029"
     CDS             15324..15923
                     /locus_tag="OQL90_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1367884.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16031..16798
                     /gene="tpiA"
                     /locus_tag="OQL90_001030"
     CDS             16031..16798
                     /gene="tpiA"
                     /locus_tag="OQL90_001030"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1367885.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="OQL90_001031"
     CDS             complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="OQL90_001031"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1367886.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="OQL90_001032"
     CDS             complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="OQL90_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1367887.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="OQL90_001033"
     CDS             complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="OQL90_001033"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1367888.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="OQL90_001034"
     CDS             complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="OQL90_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1367889.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21217..21729)
                     /gene="cpxP"
                     /locus_tag="OQL90_001035"
     CDS             complement(21217..21729)
                     /gene="cpxP"
                     /locus_tag="OQL90_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1367890.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQ
                     AEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLK
                     LLSSSNSRSQ"
     gene            21879..22577
                     /gene="cpxR"
                     /locus_tag="OQL90_001036"
     CDS             21879..22577
                     /gene="cpxR"
                     /locus_tag="OQL90_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1367891.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22574..23947
                     /gene="cpxA"
                     /locus_tag="OQL90_001037"
     CDS             22574..23947
                     /gene="cpxA"
                     /locus_tag="OQL90_001037"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1367892.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24053..24727)
                     /gene="yiiM"
                     /locus_tag="OQL90_001038"
     CDS             complement(24053..24727)
                     /gene="yiiM"
                     /locus_tag="OQL90_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1367893.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24876..25841)
                     /gene="kdgT"
                     /locus_tag="OQL90_001039"
     CDS             complement(24876..25841)
                     /gene="kdgT"
                     /locus_tag="OQL90_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS1367894.1"
                     /translation="MRKSRGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVSI
                     LAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAG
                     LSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASF
                     EPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGL
                     LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAEMVPAFKP
                     MAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26120..26740)
                     /gene="sodA"
                     /locus_tag="OQL90_001040"
     CDS             complement(26120..26740)
                     /gene="sodA"
                     /locus_tag="OQL90_001040"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1367895.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27024..28058
                     /gene="rhaT"
                     /locus_tag="OQL90_001041"
     CDS             27024..28058
                     /gene="rhaT"
                     /locus_tag="OQL90_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCS1367896.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28055..28921)
                     /gene="rhaR"
                     /locus_tag="OQL90_001042"
     CDS             complement(28055..28921)
                     /gene="rhaR"
                     /locus_tag="OQL90_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCS1367897.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(28977..29813)
                     /gene="rhaS"
                     /locus_tag="OQL90_001043"
     CDS             complement(28977..29813)
                     /gene="rhaS"
                     /locus_tag="OQL90_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1367898.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30101..31513
                     /gene="rhaB"
                     /locus_tag="OQL90_001044"
     CDS             30101..31513
                     /gene="rhaB"
                     /locus_tag="OQL90_001044"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1367899.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGLDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31510..32769
                     /gene="rhaA"
                     /locus_tag="OQL90_001045"
     CDS             31510..32769
                     /gene="rhaA"
                     /locus_tag="OQL90_001045"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1367900.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDQVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            33036..33860
                     /gene="rhaD"
                     /locus_tag="OQL90_001046"
     CDS             33036..33860
                     /gene="rhaD"
                     /locus_tag="OQL90_001046"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCS1367901.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33870..>34161
                     /gene="rhaM"
                     /locus_tag="OQL90_001047"
     CDS             33870..>34161
                     /gene="rhaM"
                     /locus_tag="OQL90_001047"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1367902.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASADVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         8370 a         8915 c         8867 g         8009 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcaccccg ccggggcaca ctgaacaggc cacgccggtg
       61 ggagcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgccgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa
      181 ccagaagcct agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt
      241 tcagaattta taaaaggcaa aattcaggca caaaaaaagc gccgtgcggc gcttttttcg
      301 gaaatccggt cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg
      361 ccaccggtag caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg
      421 tcgaggttca ggtcatgacc aacagtggag cggattttca ttacgttacc gcaagagcag
      481 ctagcagtaa tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt
      541 aaggccgcgt cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct
      601 tcaatacgat catttcgtac gaaggcgcga aatcatacag aaattaacca acgtatgcaa
      661 actgatccgc actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat
      721 gatgctatgc ccgttgccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat
      781 ctgctgccag aaggcatgac ggttaaagcc gggtgtcgcg tgcgcgtgcc gtttggcaaa
      841 cagcaggagc gcatcggggt tgtggtgtca gttagcgatg tcagtgagct gccgcttaac
      901 gagctgaaag cggtagtgga agtgctggac gttgagccgg tatttactca ctccgtctgg
      961 cggttactgc tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat
     1021 gccttgccga ttttactgcg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg
     1081 tttgccactg aacaaggcca ggcggtggat ctgaacagcc tgaaacgctc ccccaagcaa
     1141 caacaggcgc tggcggcgtt acggcaaggc aaaatctggc gcgaccaggt cgccacgctc
     1201 gaatttaatg atgccgcgtt gcaggcgcta cgcaaaaaag gtctgtgtga tttagcaagt
     1261 gaaacaccag agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga
     1321 ttgaataccg aacaggccac cgccgttggc gcaattcata gcgcggcaga tactttttct
     1381 gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg
     1441 gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca
     1501 ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc
     1561 ggcctgaacg acagcgagcg gctttctgcg tggttgaaag cgaaaaatgg cgaggcggcg
     1621 attgtgatcg gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc
     1681 attgatgaag agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc
     1741 gacctggcgg tgtatcgtgc gcacagcgag caaatcccga ttattcttgg ctccgcaacg
     1801 cccgcgctgg aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacc
     1861 cgtcgggcag ggaatgctcg tccggcaatt caacatgtgc tggatttaaa aggtcagaag
     1921 gtgcaggcag gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac
     1981 aatcaggtta tcctctttct taatcgtcgg ggttttgcgc ctgcgctgct gtgccacgac
     2041 tgcggctgga tagccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa
     2101 cagcatttgc gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc
     2161 tgcggttcca cgcacctggt ccccgtgggg ctgggcaccg aacagcttga acagacgctt
     2221 gcgccgatgt tccccggcgt gcccatttca cgtatcgacc gcgataccac cagccgcaaa
     2281 ggggcactgg aacagcaact ggcggaagtg catcgcggcg gcgcgaggat tttgatcggc
     2341 acacaaatgc tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat
     2401 gtggacggcg cgctgttttc tgccgatttt cgctcggcag aacgtttcgc ccagctttac
     2461 acccaggtcg ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg
     2521 caccatccgg aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc
     2581 gaacaggcgc tggctgagcg acgcatgatg cagctgccgc cgtggaccag ccatgtgatt
     2641 gtgcgtgcgg aagatcataa caatcagcac gcgccgctgt tcctgcaaca actgcgtaat
     2701 ctgatccttt ccagcccgct ggcagacgac aaactgtgga ttctcggccc ggttccggct
     2761 ctggcaccta agcgtggtgg ccgctggcgc tggcagatct tgttgcagca cccttcccgc
     2821 gtgcgcttgc aacacatcat tagtggtacg ctggcgctta ttaatacaat accggattcc
     2881 cgcaaggtga aatgggtgtt ggatgtcgat cccatcgaag gctaatgcgc aaacgttgcg
     2941 aggcggatcg aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc
     3001 acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac
     3061 gtaccaggcg aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag
     3121 acgttgccct caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg
     3181 ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt
     3241 taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg
     3301 tcccggatat ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg
     3361 caaatcacgg atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa
     3421 cctttatcga tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc
     3481 tgccgtttga tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg
     3541 aatttgcacc ggaactggag ctgcctaccg ttcatatcga caatctgacc gccgcatttg
     3601 atgcagtaaa ttatttatat gagcaagggc ataaacggat tggctgtata gccggtcccg
     3661 aagagatgcc actgtgtcac taccgcctgc aaggctatgt tcaggcgctg cgtcgctgcg
     3721 gaattatggt tgatccacaa tacatcgccc gtggcgactt caccttcgaa gccggaagca
     3781 aagcaatgca acagctgctt gatcttccac aaccgcctac tgcagtcttc tgccatagcg
     3841 atgtgatggc gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag
     3901 acctttccat aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga
     3961 caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc
     4021 aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc
     4081 ggggatcaac acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag
     4141 gcccgccgct taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg
     4201 gcacaacgag attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc
     4261 tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc
     4321 gccgttcttg tgacctttat cggtggtctg tacttcatta cgcatcacaa taaagaagag
     4381 tccgagacgc tgcaaagcca gaaagtgacc ggaaacggac taccaccaaa accagaagaa
     4441 cgctggcgct acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa
     4501 ccttctgccg gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt
     4561 cttgaacaaa tgcaggctga tatgcgccag cagccaacgc agctggttga ggttccgtgg
     4621 aatgaacaga cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa
     4681 ttagcggagc agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag
     4741 cagacgcgta cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccagct
     4801 tctactcagc agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg
     4861 aagccgcagc aagctgcacc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag
     4921 aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca
     4981 gagacggtac gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat
     5041 ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc
     5101 aatcggttga agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac
     5161 cctcaaaatc ccccccatct ataattgcat tatgcctcgt acttttgtac ggggtttgta
     5221 ctctgtattc gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg
     5281 gccatgtggt catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca
     5341 acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta
     5401 ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc
     5461 atctggttaa agccgccgtt gagctggcaa aagactggcg taccgatcgc atgctgcgca
     5521 aacttgaagc actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg
     5581 gtgacgtggt gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc
     5641 aggctgcggc gcgcgcgctg ttagaaaaca ctgaacttag cgcccgtgaa attgctgaaa
     5701 aggcgttgga tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag
     5761 aattaagcta caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca
     5821 gcgaactgga taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc
     5881 tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga
     5941 aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg
     6001 ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtgggct
     6061 atgtcggtaa ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg
     6121 tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta
     6181 ttctcgacgt gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg
     6241 aaccgtccgc tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca
     6301 aagaaatcga gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag
     6361 gcatggaaga gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc
     6421 aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg
     6481 cgaaactggt gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg
     6541 ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg
     6601 atgtttctcg tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt
     6661 ccaccaaaca cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc
     6721 agattgcgaa accgtctgac ctaatcccgg aactgcaagg tcgtctgcca atccgcgttg
     6781 aactgcaggc actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta
     6841 tcaccgtgca gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact
     6901 ccggtattaa acgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg
     6961 gtgctcgtcg tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca
     7021 gcgatttaag cggtcaaaat atcactattg acgcagatta tgtgagcaaa catctggatg
     7081 cgttggtggc agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca
     7141 tcattgtttg atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac
     7201 aaattagccg aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct
     7261 ttgctgcaat tatcgtcggg acggcgctgg catggtggca aggtcatttt gatccgctgg
     7321 tcgccctgct ggcactgatt accgccgggc tattacagat cctttctaac ctcgccaatg
     7381 attacggcga tgcggtaaaa ggcagcgata gacctgaccg cattgggccg ctacgcggca
     7441 tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc
     7501 tcatctgtct ctccgggctg gcactggttg cagtggcgtg ccatacgctg gctgattttg
     7561 tcggtttcct gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca
     7621 atcgtcctta tggttatatc ggtctggggg atatttccgt actggttttc tttggctggt
     7681 taagcgtcat ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc
     7741 cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa cattaataac ctgcgtgata
     7801 tcaatagcga ccgagaaaac ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg
     7861 cgcgtcgtta tcatgcctgc ctgctgatgg gctctctggt atgtctggcg ctgtttaatc
     7921 tcttttcgct gcatagcctg tggggctggc tgttcctgct ggcggcaccg ttgctggtga
     7981 agcaagcccg ttatgtgatg cgggaaatgg acccggtggc gatgcgaccg atgctggaac
     8041 gcactgtcaa gggggcgtta ctgactaacc tgctgtttat tttagggata ttcctaagcc
     8101 agtgggcagc ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca
     8161 tagcgcgata tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac
     8221 ttccgagctt tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa
     8281 ctttggcgga cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa
     8341 cgggttgctg tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg
     8401 cggtggttct gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa
     8461 tgaatgggaa ggtctggtta tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt
     8521 ggatatcggc atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg
     8581 cgaaagcgat gtccgcgtca attttggcgg tgtaaccttc ttctccggcg accatcttta
     8641 tgccgacaat accgggatta ttctttcaga agatccgctg gatattgaat gataagaaag
     8701 gcaccgcaag gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc
     8761 gaaagatgaa gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct
     8821 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
     8881 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
     8941 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
     9001 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaaat
     9061 cctcgtcccg attaccggtg acgccttaat aaatacgagc gcactttagt tagctccgat
     9121 tgtatgaagc cgcgccatcg ctgtccagcg gcacgccttg cagattacgg tttgccacac
     9181 ttttcatcct tctcctggtg acataatcca catcaatcga aaatgttaat aaatttgttg
     9241 cgcgaatgat ctaacaaaca tgcatcatgt acaatcagat ggaataaatg gcgcgataac
     9301 gctcatttta tgacgaagca cacacatttt aagttcgata tttctcgttt ttgctcgtta
     9361 acgataagtt tacagcatgc ctacaagcat cgtggaggtc cgtgactttc acgcatacaa
     9421 caaacattaa ctcttcagga tccgattatg agtcaaacat caaccttgaa aggccagtgc
     9481 attgctgaat tcctcggtac cgggttgttg atttttttcg gtgtgggttg cgttgcagca
     9541 ctaaaagtcg ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg gggactgggg
     9601 gtggcaatgg ctatctacct gaccgcaggg gtttccggcg cgcatcttaa tcccgctgtt
     9661 accattgcat tgtggctgtt tgcctgtttc gacaagcgca aagttattcc ttttatcgtt
     9721 tcacaagttg ccggcgcttt ctgtgctgcg gctttagttt acgggcttta ctacaattta
     9781 tttttcgact tcgagcagac tcatcacatt gttcgcggca gcgttgaaag tgttgatctg
     9841 gcaggcactt tctctactta ccctaatcct catatcaatt ttgtgcaggc tttcgcagtt
     9901 gagatggtga ttaccgctat tctgatgggg ctgatcctgg cgttaacgga cgatggcaac
     9961 ggtgtaccac gcggcccttt ggctcccttg ctgattggtc tactgattgc ggtcattggc
    10021 gcatctatgg gcccattgac gggttttgcc atgaacccag cgcgtgactt cggtccgaaa
    10081 gtctttgcct ggctggcggg ctggggcaat gtcgccttta ccggcggcag agacattcct
    10141 tacttcctgg tgccgctttt tggccctatc gttggcgcga ttgtaggtgc atttgcctac
    10201 cgcaaactga ttggtcgcca tttgccttgc gatatctgtg ttgtgaaaga aaaggaaacc
    10261 acaactcctt cagaacaaaa agcttcgctg taatatgact acgggacaat taaacatgac
    10321 tgaaaaaaaa tatatcgttg cgctcgacca gggcaccacc agctcccgcg cggtcgtaat
    10381 ggatcacgat gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa tctacccaaa
    10441 accaggttgg gtagaacacg acccaatgga aatctgggcc acccaaagct ccacgctggt
    10501 agaagtgctg gcgaaagccg atatcagttc cgatcaaatt gcagctatcg gtattacgaa
    10561 ccagcgtgaa accactattg tctgggaaaa agaaaccggc aagcctatct ataacgccat
    10621 tgtctggcag tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg acggtttaga
    10681 agattatatc cgcagcaata ccggtctggt gattgacccg tacttttctg gcaccaaagt
    10741 gaagtggatc ctcgaccatg tggaaggctc tcgcgagcgt gcacgtcgta gtgaattgct
    10801 gtttggtacg gttgatacgt ggcttatctg gaaaatgact cagggccgtg tccatgtgac
    10861 cgattacacc aacgcctctc gtaccatgtt gttcaacatc cataccctgg actgggacga
    10921 caaaatgctg gaagtgctgg atattccgcg cgagatgctg ccagaagtgc gtcgttcttc
    10981 cgaagtatac ggtcagacta acattggcgg caaaggcggc acgcgtattc caatctccgg
    11041 gatcgccggt gaccagcagg ccgcgctgtt tggtcagttg tgcgtgaaag aagggatggc
    11101 gaagaacacc tatggcactg gctgctttat gctgatgaac actggcgaga aagcggtgaa
    11161 atcagaaaac ggcctgctga ccaccatcgc ctgcggcccg actggcgaag tgaactatgc
    11221 gttggaaggt gcggtgttta tggcaggcgc atccattcag tggctgcgcg atgagatgaa
    11281 gttgattaac gacgcctacg attccgaata tttcgccacc aaagtgcaaa acaccaatgg
    11341 tgtgtatgtg gttccggcat ttaccgggct gggtgcgccg tactgggacc cgtatgcgcg
    11401 cggggcgatt ttcggtctga ctcgtggggt gaacgctaac cacattatac gcgcgacgct
    11461 ggagtctatt gcttatcaga cgcgtgacgt gctggaagcg atgcaggccg actctggtat
    11521 ccgtctgcac gccctgcgcg tggatggtgg cgcagtagca aacaatttcc tgatgcagtt
    11581 ccagtccgat attctcggca cccgtgttga gcgcccggaa gtgcgcgaag tcaccgcatt
    11641 gggtgcggcc tatctcgcag gcctggcggt tggcttctgg cagaacctcg acgagctgca
    11701 agagaaagcg gtgattgagc gcgagttccg tccaggcatc gaaaccactg agcgtaatta
    11761 ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg gcgtgggaag aacacgacga
    11821 gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcctgaa acgtgcgggg
    11881 gcaaccccgc acacatcaat aatcccaccc ttcccctgtg ctacacttcg cgccattcct
    11941 tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa
    12001 tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc
    12061 gcggcggtaa acgccatgcg tattatgctc aaccaggtca acattgacgg caccatcgtc
    12121 attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact
    12181 ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg
    12241 atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat
    12301 gcgccggata tgtatatgga gaagctgatt gtcgggccgg gagccaaagg caccattgat
    12361 ctgaacctgc cgctggcgga taacctgcgc aatgtagcgg cggcgctcgg caaaccgttg
    12421 agcgaactga cggtaacgat tctagctaaa ccacgccacg atgccgttat cgctgaaatg
    12481 cagcaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc
    12541 acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc
    12601 gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg
    12661 cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca
    12721 cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc
    12781 agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt
    12841 agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc
    12901 attcgccgca ttcagtccat ccactatctg gatcgcaaag acccggaaat gcaggtgcac
    12961 atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt
    13021 tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc tgattgggta
    13081 acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac
    13141 gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc
    13201 gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt
    13261 tacctggtca ccgtccccga tggcaaatta agcccacgat tggcggcact gaaaccaggc
    13321 gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggatga agtaccagat
    13381 tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg
    13441 caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat
    13501 gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggcaaa
    13561 ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cggacggata
    13621 ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa
    13681 accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg
    13741 aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag
    13801 cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct
    13861 tattgatcat cccgcgatat gctggcatca aacggttgat aaatgagtga tgatgaccac
    13921 agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc
    13981 cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct
    14041 tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac
    14101 ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat
    14161 gtctggcgag ctccagagct ttattcacca gtaaggcatc ttcttcattc ccggaaattg
    14221 ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa
    14281 cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc
    14341 atcaagctca acttgcccct ccagatcctt aatcttgtag gccacgctgg ccccttcatc
    14401 tacagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc
    14461 gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc
    14521 gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct
    14581 ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac
    14641 aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc
    14701 agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata
    14761 gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat
    14821 cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga
    14881 taagcatcat caccagaatc aacgtcggga caaaacaccc caccgcccat tgccagacac
    14941 ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc
    15001 atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct gttggccaaa
    15061 gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg
    15121 agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc
    15181 caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt
    15241 tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc
    15301 agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt
    15361 ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc
    15421 cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact
    15481 ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac agcagtcccg
    15541 acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag
    15601 cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg
    15661 ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca
    15721 cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctgctaacgg
    15781 cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg
    15841 acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat
    15901 cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac
    15961 tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg
    16021 gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca
    16081 catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc
    16141 ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag
    16201 ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga
    16261 aacctctgct gctatgctga aagacatcgg cgcacagtac atcatcatcg gccactctga
    16321 acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa
    16381 agagcagggt ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg
    16441 caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc
    16501 ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc
    16561 tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt
    16621 tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa
    16681 cgctgcagaa ctgtttgctc agccagacat cgacggcgcg ctggttggcg gtgcgtctct
    16741 gaaagctgac gctttcgcag taatcgttaa agctgcagaa gcggctaaac aggcttaagt
    16801 ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca
    16861 aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat
    16921 ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt
    16981 agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc
    17041 gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac
    17101 ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca
    17161 catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg
    17221 agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtacttt ttgctcataa
    17281 aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag
    17341 gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt gggccattaa
    17401 gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt
    17461 gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact
    17521 cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata actatcatca
    17581 ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag
    17641 ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa
    17701 ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc
    17761 tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt
    17821 ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta gtagtttttc
    17881 gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct
    17941 ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata
    18001 gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga
    18061 gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg
    18121 gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc
    18181 tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag
    18241 ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta
    18301 atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg
    18361 gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc
    18421 ccgcgttccg caattgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca
    18481 ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgcatcacc
    18541 acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac
    18601 aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg
    18661 gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat
    18721 gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa
    18781 gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga aatccgcctc
    18841 atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc
    18901 tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc
    18961 atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct ttttccgaaa
    19021 tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt
    19081 ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg
    19141 accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat
    19201 acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt
    19261 ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc
    19321 ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac
    19381 caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat
    19441 ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg
    19501 ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc
    19561 agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag
    19621 accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc
    19681 gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag ccacggcgcg
    19741 gatgttctcg tcgcggaatt ccgggaaacg cgcagaaccg aggaacgtac cgccacggtt
    19801 gatcatgtca gacacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag
    19861 atagccgtca taaataccca ttacttccag accttctgtc agcgcagaac gaacaacccc
    19921 gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt
    19981 aatcatgact acctctgaac tttggaatgc aaaatgaaat ctgttgccgg aagtcttctt
    20041 gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg
    20101 attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta
    20161 tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg
    20221 atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc
    20281 caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa
    20341 gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga
    20401 agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga
    20461 ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa
    20521 tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat
    20581 tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct
    20641 ttgcgtccgg cgcaccaccc aacgctgaaa cgagacaagg ataatcgtac aaattagcgc
    20701 cacaattgtc acgataaccc cgacgcctgg atctatcatc ggtgttggag atatcagatg
    20761 ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag
    20821 cgccgcgagg gactctgctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag
    20881 ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac
    20941 cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa
    21001 tagcaacgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga
    21061 ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca aatcatctgc
    21121 aatgcattaa gcagcaggca aattgaggat aaaaaaaacc ccacatcatg tgggggaaga
    21181 cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg agttgctact
    21241 actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcacgca actgctccat
    21301 tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat
    21361 ttggttgcgg acttttgcca tctcaacctg acgagcaatt tgctcattcg ccattttttc
    21421 tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc gatgcattgt
    21481 ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca taagatctcg
    21541 catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc tctgcgtact
    21601 gcgctgcgta ctgcgctgcg taagttcttc acccggatgc cagttatcgc ctgaaccgac
    21661 ttcagcagcg tggcttaatg aactgactgc cagcgttgaa gccatgacgg cagcggtaac
    21721 tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt caacgataga gagtttacga
    21781 ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg attagcgacg
    21841 tctgatgacg taatttctgc ctcggaggta tttaaacaat gaataaaatc ctgttagttg
    21901 atgatgaccg agagctgact tccctattaa aggagctgct cgagatggaa ggcttcaacg
    21961 tgattgttgc ccacgatggg gaacaggcgc ttgatcttct ggacgacagc attgatttac
    22021 ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa gcacttcgcc
    22081 agacacacca gacgcctgtc attatgttga cggcgcgcgg cagtgaactt gatcgcgttc
    22141 tcggccttga gctgggcgca gatgactatc tcccgaaacc gtttaatgat cgtgagctgg
    22201 tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag cgagcaacag caaaacaacg
    22261 acaacggttc accgacactg gaagttgatg ccttagtgct gaatccaggc cgtcaggaag
    22321 ccagcttcga cgggcaaacg ctggagttaa ccggtactga gtttaccctg ctctatttgc
    22381 tggcacagca tctgggtcag gtggtttccc gtgaacattt aagccaggaa gtgttgggca
    22441 aacgcctgac gcctttcgac cgcgctattg atatgcacat ttccaacctg cgtcgtaaac
    22501 tgccggatcg taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc ggctatctga
    22561 tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct tctggctgac
    22621 gctggcgctg gtgttgatgt tggttttgat gttacccaag ctcgattcac gccagatgac
    22681 cgagcttctg gatagcgaac agcgtcaggg gctgatgatt gagcagcatg tcgaagcgga
    22741 gctggcgaac gatccgccca acgatttaat gtggtggcgg cgtctgttcc gggcgattga
    22801 taagtgggca ccgccaggac agcgtttgtt attggtgacc accgaaggcc gcgtgatcgg
    22861 cgctgaacgc agcgaaatgc agatcattcg taactttatt ggtcaggccg ataacgccga
    22921 tcatccgcag aagaaaaagt atggccgcgt ggaactggtc ggtccgttct ccgtgcgtga
    22981 tggcgaagat aattaccaac tttatctgat tcgtccggcc agcagttctc aatccgattt
    23041 cattaactta ctgtttgacc gcccgctatt actgctgatt gtcaccatgt tggtcagtac
    23101 gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc tgaaaaacgc
    23161 tgccgatgaa gttgcccagg gaaacttacg ccagcacccg gaactggaag cggggccaca
    23221 ggaattcctt gccgcaggtg ccagttttaa ccagatggtc accgcgctgg agcgcatgat
    23281 gacctctcag cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc cgctgacgcg
    23341 tctgcaactg ggtacggcat tactgcgccg tcgtagcggt gaaagcaagg aactggagcg
    23401 tattgaaacc gaagcgcaac gtctggacag catgatcaac gatctgttgg tgatgtcacg
    23461 taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa gccaaccagt tgtggagtga
    23521 agtgctggat aacgcggcgt tcgaagccga gcaaatgggc aagtcgttga cagttaactt
    23581 cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc ctggaaagtg cgctggaaaa
    23641 cattgttcgt aatgctctgc gttattccca tacgaagatt gaagtgggct ttgcggtaga
    23701 taaagacggt atcaccatta cggtggacga cgatggtcct ggcgttagcc cggaagatcg
    23761 cgaacagatt ttccgtccgt tctatcggac cgatgaagca cgcgatcgtg aatctggcgg
    23821 tacaggattg gggctggcga ttgttgaaac cgccattcag cagcatcgtg gctgggtgaa
    23881 ggcagaagac agcccgctgg gcggtttacg gctggtgatt tggttgccgc tgtataagcg
    23941 gagttaaact ccgcatttgt aggcaggata aggcgtttac gccgcatccg gcatttgagc
    24001 aggatgcctg atgcgacgct gatagcgtct tatcaggcct acactccggg ggttattttc
    24061 cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt tgcatcgtcc
    24121 gcgtccagct tttcgataac ccggcggcgg agagtaaacg gtgatactga tcgtcatcaa
    24181 acggcatatg ccatgcgatg gcggcagcct cctgcacggt cacatcgctg acacgggaaa
    24241 ccaactccag cggcgcgtcc gcagacactt tccctggcgc tatcacgcta tacaaccacc
    24301 ccaccttacc tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa tggtaattga
    24361 gtttatagca aggcgagcgc ggctggctga cctgaattaa tgcctctccc cagcggaaaa
    24421 tatcgcccat ataaacatta ctttccgtca ggccgtcggt tgagaggttt tcaccaaacg
    24481 caggcgcaac aaacaactcc gcctgttccg gaaattcccg cgcccagtag agataatgct
    24541 cacgaggata atgacacagc gctctgtctg gcccaccatg aacctttttc tccgcctgct
    24601 cgtcaccttc cagccccagc tccgtcagca tcaactcacc atcaacctgg attttggcaa
    24661 ttgcgctggg tttgccttcg ggataagcct gaatcttgcc tgtgtataca tcaaccggat
    24721 atcgcatctg ttccctctcc acgagaaatt tccccattag accatattta ttttatgtca
    24781 ggcgtgctgt tttcgcggta agtatccagg caggaactca atagcaaacc ggacgaacgt
    24841 tagttcatcc ggctgtgggg attaaggaag caggtttatt tcaccgtacc taaaatttcg
    24901 attttcgctg ctctggcttt gactttacgt gaccagatag aggtaagaat tggcaccaga
    24961 atcgaagtca caatgaccgc cgtcgctacc agcgaagttg ctgccggagc catcggttta
    25021 aacgcaggca ccatttctgc aatcagcata ggtgtcgcta ccgcggcccc tgcggaactg
    25081 gaagcggcaa ttccggcagt gccatcgcca ccgccaatca atttatcggc gataatcaac
    25141 ggaataccgg tcacgataat tactgccaca cccaacagga tccccagcaa accagtctgg
    25201 gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt
    25261 tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa ggttccccag ggcaaagccc
    25321 accaggaacg gtaatactgc gccgacgaaa acatgcggtt caaacgaggc aatccctgca
    25381 gtgcccagaa taatcatcgt catgagcggc ccggactcca acgacatcaa cacaaatgcc
    25441 ccagcttctt cttttgtgcc gtactgctgc atgatggaag cgtaaagtcc gccattggtc
    25501 atatccatcg ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca
    25561 ccatgttccg gaataatgcg cgaggcaatt gccgcaacca cccacgcgac ggcaattttt
    25621 gttaccacca gcgtaccgga tttacgcaat accgttcccg tcgcgcttaa ttttattgac
    25681 gcccccatgc aaaaaaacca caccgccaga atggacaccg taccggtaat catcccgttg
    25741 gtgaatgatc caaaatattt ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg
    25801 aatagcggga cgagcatcat ccccccccgg gattttctca atcgagcgtt ttatctgcat
    25861 ttccatcacc tgtcggcata tttttaaggg ctgatgcgta attcctcaat ggagcatcag
    25921 tgttgatgga aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca
    25981 attgaaacgc tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa
    26041 aaaccaccgc cgttggcgat ggttcatttt ttaagctgat atgcggcgta aacgcctcat
    26101 tgcagcaggc ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct tcatcccagt
    26161 tcaccacgtt ccagaactct ttgatgtagt ccggacggcg gttctggaat ttcaggtagt
    26221 aagcatgttc ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt
    26281 cacccatcag cggggaatcc tggttagcag tagaaaccac cgccagttta tcgcctttca
    26341 gcaccagcca tgcccagccg gaaccaaagc gggaagctgc cgctttctca aattctgctt
    26401 tgaagttatc aacggagccg aagtcacgtt cgatagccgc tttcaggtca ccctgtaagg
    26461 tggtgccttt tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt
    26521 tgcgcagtac ggttttcttg tctgctggca gctggtccag tttggtgatc agctcttcaa
    26581 ccggcaggtt ggcaaattct ggcaggcttt ccagcgccgc gttggcgttg ttgacgtagg
    26641 tctgatggtg tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg
    26701 catcgtaagc atacggcagg gatggcaggg tatagctcat attcatctcc agtattgtcg
    26761 ggcggccgat tgttaatgcg cgtaagcagt tggttcatta tagttaatta aatgatattg
    26821 aaaatgatta tcaatgccgt acttttcgta agggtatggt tttgcaggaa aattcccgag
    26881 atgtgaagca aatcacccac ttaatgccgt gattgccagt aaatcgacaa cggcggcaac
    26941 aggcgaaagg ttaatcgaca gcacgatttt tacactcatc tcgtcggaga tgtgacgcga
    27001 cgaaaaatga tgaggataag aagatgagta acgcgattac gatggggata ttttggcatt
    27061 tgatcggcgc ggccagtgca gcctgttttt acgctccgtt caaaaaagta aaaaaatggt
    27121 catgggaaac catgtggtca gttggtggga ttgtttcgtg gattattctg ccgtgggcca
    27181 tcagcgccct gttactgccg aatttctggg cgtattacag ctcgtttagt ctctctacgc
    27241 tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg taatatcaac tacggcctga
    27301 ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc cattggcatt acgttgattg
    27361 tcggtacgct gatgacgcca attatcaacg gcaatttcga tgttctgctt aataccgaag
    27421 gcggacgcat gacgttgctc ggcgttctgg tggcgctgat tggtgtaggg attgtgactc
    27481 gcgccgggca gttgaaagag cgcaaaatgg gcattaaagc cgaagagttc aacctgaaaa
    27541 aagggctggt gctggcggtg atgtgcggca ttttctctgc cggtatgtct tttgcgatga
    27601 acgccgcaaa accgatgcat gaagccgctg ccgcactggg cgtcgatcca ctgtatgtcg
    27661 ctctgccaag ctatgtcatc atcatgggcg gcggcgcgat catcaacctc ggtttctgct
    27721 tcattcgtct ggcaaaagtg aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac
    27781 cgctgatcat tcacaatgtg ttactctcag cactgggcgg gttgatgtgg tatctgcaat
    27841 tctttttcta tgcctggggc cacgcccgca ttccggcgca gtatgactac atcagttgga
    27901 tgctgcatat gagtttctat gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt
    27961 ggaacaatgc aggtcgccgt ccggtaacgg tgttgagcct cggttgtgtg gtgattattg
    28021 tcgccgccaa catcgtcggc atcggcatgg cgaattaatc tttctgcgaa ttgagatgac
    28081 gccactggct gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa tagttactat
    28141 cttcaaagcc acattcggtc gaaatatcac tgattaacag gcggctatgc tggagaagat
    28201 attgcgcatg acacactctg acctgccgca gatattgatt gatggtcatt ccagtctgct
    28261 ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa aatttatcca
    28321 gcgcaaaggg acttttcagg ctagccgcca gccgggtaat cagcttatcc agcaacgttt
    28381 cgctggatgt tggcggcaac gaatcactgg tgtaacgatg gcgattcagc aacatcacca
    28441 actgcccgaa cagcaactca gccatttcgt tagcaaacga cacatgctga ctactttcat
    28501 gctcaagctg accgataacc tgccgcgcct gcgccatccc cacgctacct aagcgccagt
    28561 gtggttgccc tgcgctggcg ctaaatcccg gaatcgctcc ctgccagtca agattcagct
    28621 tcagccgctc cgggcaataa ataatattct gcaaaaccag atcgttaacg gaagcgtagg
    28681 agtgtttatc gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga taagggcgat
    28741 cgttgagtac atgcagacca ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg
    28801 tatgttcggc aaaaacatct tgcggataac ggtcagccac agcgactgcc tgctggtcgc
    28861 tggcaaaaaa atcatctttg agaagtttta actgatgcgc caccgtggct acctcggcca
    28921 gagaacgaag ttgattattc gcaatatggc gtacaaatac gttgagaaga ttcgcgttat
    28981 tgcagaaagc catcccgtcc ctggcgaata tcacgcggtg accagttaaa ctctcggcga
    29041 aaaagcgtcg aaaagtggtt actgtcgctg aatccgcagc gataggcgat gtcagtaacg
    29101 ctggcctcgc tgtgacgtag cagatgtcgg gccttcatca gtcgcaggcg gttcaggtaa
    29161 cgctgaggcg tcagtcccgt ttgctgctta agctgccgat gtagcgtacg cagtgaaaga
    29221 gaaaattgat ccgccacggc atcccaattc acctcatcgg caaaatggtc ctccagccag
    29281 gccagaagca agttgagacg tgatgcgctg ttttccaggt tctcctgcaa actgctttta
    29341 cgcagcaaga gcagtaattg cataaacaag atctcgcgac tggcggtcga gggtaaatca
    29401 ttttcccctt cctgctgttc catctgtgca accagctgtc gcacctgctg caatacgctg
    29461 tggttaacgc gccagtgaga cggatactgc ccatccagtt cttgtggcag caactgattc
    29521 agcccggcga gaaactgaaa tcgatccggc gagcgataca gcacattggt cagacacaga
    29581 ttatcggtat gttcatacag atgccgatca tgatcgcgta cgaaacagac cgtgccaccg
    29641 gtgatggtat agggctgccc attaaacaca tgaatacccg tgccatgttc gacaatcaca
    29701 atttcatgaa aatcatgatg atgttcagga aaatccgcct gcgggagccg gggttctatc
    29761 gccacggacg cgttaccaga cggaaaaaaa tccacactat gtaatacggt catactggcc
    29821 tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt caggttctta ccttaaattt
    29881 tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa
    29941 atgcggtgag catcacatca ccacaatgaa gcaaattgtg aacatcatca cgttcatctt
    30001 tccctggttg ccaatggccc attttcctgt cagtaacgag aaggtcacga attcaggcgc
    30061 tttttagact ggtcgtaatg aaattcagca ggatcacatt atgacctttc gcaattgtgt
    30121 cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg
    30181 ccgcagcctg acgctgcgcg aaatccatcg ttttaacaat ggcctgcata gccagaacgg
    30241 ttatgtcacc tggaatgtgg atagcctgga aagtgccatt cgccttggat taaacaaggt
    30301 gtgcgaggaa gggattcgta tcgatagcat tgggcttgat acctggggcg tggactttgt
    30361 gctgctcgac caacagggtc agcgtgtggg cctgcccgtt gcttatcgcg atagccgctc
    30421 caatggccta atggcgcagg cacagcaaca actcggcaaa cgcgatattt atcaacgtag
    30481 cggcatccag tttctgccct tcaatacgct ttatcaactg cgtgcgctga cggagcaaca
    30541 acctgaactt attccacaca ttgctcacgc tctgctgatg ccggattact tcagctatcg
    30601 cctgaccggc aagatgaact gggaatatac caatgccacc accacacaac tggtcaatat
    30661 caatagcgac gactgggacg agtcgctact ggcgtggagc ggggccaaca aagcctggtt
    30721 tggtcgcccg acgcatccgg gtaatgtcat tgggcactgg atttgcccgc agggtaatga
    30781 gattccggtg gtcgccgttg ccagccatga taccgccagc gcggttatcg cctcgccgtt
    30841 aaacggttca cgcgctgctt atctctcttc tggcacctgg tcattgatgg gcttcgaaag
    30901 ccagacgcca tttaccaatg acacggcgct ggcagccaac ataaccaatg aaggcggggc
    30961 ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta tggctgcttc agcgagtgct
    31021 tcaggagcgg caaatcaacg atctcccggc gcttatcgcc gcgacacagg cacttccggc
    31081 ctgccgcttc atcatcaatc ccaatgacga tcgctttatt aatcctgagg cgatgtgcag
    31141 cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc ccggaaagtg atgctgaact
    31201 ggcgcagcta cgcggtgaag atttctcgca actgcatatt gtcggcggcg gctgccagaa
    31261 cacgctgctc aaccagctat gtgccgatgc ctgcggtatt cgggtgatcg ccgggcctgt
    31321 tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg aactcaacaa
    31381 tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct ttacccctaa
    31441 tcctgacagt gaaattgccc actatgtggc gcagcttcac tctacacgac agacaaagga
    31501 gctttgcgca tgaccactca actggaacag gcctgggagc tggcgaaaca gcgtttcgcg
    31561 gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc cgtttcaatg
    31621 cactgctggc agggcgatga tgtttccggt tttgaaaacc cggaaggttc gctgaccggg
    31681 gggattcagg ccacaggcaa ttatccgggc aaagcgcgta atgccagtga gctacgtgcc
    31741 gatctggaac aggctatgcg gctgattccg gggccgaaac ggcttaattt acatgccatc
    31801 tatctggaat cagatacgcc agtctcgcgc gaccagatca aaccagagca cttcaaaaac
    31861 tggggtgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc ctgcttttcg
    31921 catccgctaa gcgccgatgg ctttacgctc tcgcatcccg acgacagcat tcgccagttc
    31981 tggattgatc actgcaaagc cagccgtcgc gtttcggcct attttggcga gcaactcggc
    32041 acaccatcgg tgatgaacat ctggatcccg gatggtatga aagatatcac cgttgaccgt
    32101 ctcgccccgc gtcagcgtct gctggcagca ctggatgagg tgatcagcaa gaagctaaac
    32161 cctgcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc agagagctac
    32221 acggttggct ccaatgagtt ttacatgggg tatgccacca gccgccagac tgcgctgtgc
    32281 ctagacgccg ggcacttcca cccgactgaa gtgatttccg acaagatttc cgccgccatg
    32341 ctgtatgtgc cgcagttgct gctgcacgtc agccgtccgg ttcgctggga ctgcgatcac
    32401 gtagtgctgc tggatgatga aacccaggca attgccagtg agattgtgcg tcacgatctg
    32461 tttgaccagg tgcatatcgg ccttgacttc ttcgatgcct ctatcaaccg cattgccgcg
    32521 tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct ggaacctacc
    32581 gctgagctgc gcaagctgga agcggcgggc gattacactg cgcgtctggc actgctggaa
    32641 gagcagaaat cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg tcacgatacg
    32701 ccagcaggta gcgaatggct ggagagcgtg cgggcttatg aaaaagaaat tttgagccag
    32761 cgtgggtaaa cactgccgga tgcggcgcga gcgccttatc cggcctacgg gtcggcaaca
    32821 gttgtaggcc tgataagacg cgacagcgtc gcatcaggca ttgattgccg gatgcggcac
    32881 gagtgcctta tccggcctac aggtcggcaa tagttgtagg cctgataaga cgcgacagcg
    32941 tcgcatcagg cattgattgc cggatgcggc acgagcgcct tatccggcct acgggtcgtg
    33001 catctgacaa caccgaattt acaggaacac agaacatgca aaacattacc cagtcctggt
    33061 ttgtccaggg aatgatcaaa gccaccaccg atgcctggct gaaaggctgg gacgaacgca
    33121 acggcggcaa cctgacgctg cgcctggatg acgccgatat cgcgccttat aaagacaacg
    33181 tccatgcaca accgcgctat atcccgctca gccagcccat gcctttactg gcaaatacac
    33241 cgtttattgt caccggctcc ggcaaattct tccgtaacgt ccagctcgat cctgcagcta
    33301 acttaggcgt cgtaaaagtc gacagcgatg gcgcgggcta ccatattctc tgggggttaa
    33361 ccaacgaagc cgtcccgaca tccgaactcc cggcgcactt tctctcccac tgcgagcgca
    33421 ttaaagccac caacggtaaa gatcgggtga tcatgcactg ccacgccacc aacctgatcg
    33481 ccctcaccta tgtactggaa aacgacaccg cggtctttac tcgccaactg tgggaaggca
    33541 gcaccgagtg tctggtggta ttcccggatg gcgttggcat tctgccgtgg atggtgcccg
    33601 gcacggacga aatcggccag gcgaccgcac aagagatgca aaaacattcg ctggtgttgt
    33661 ggcccttcca cggcgtcttc ggcagcgggt cgacgttgga tgaaacattc ggtttaatcg
    33721 acaccgcaga aaaatcagca caagtattag tgaaggttta ttcgatgggc ggcatgaaac
    33781 agaccatcag ccgtgaagag ttgatagcgc tcggcaagcg ctttggcgtt acaccactag
    33841 ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc atgcaggtta
    33901 accccgacgc ccacgaagag tatcagcgtc ggcataaccc catctggccg gaactggaag
    33961 cagtgttgaa atctcacggt gcgcataact acgccattta cctcgacaaa gcgcgtaatc
    34021 tgctgtttgc cacggtagag attgaatctg aagaacggtg gaatgcggta gccagtgctg
    34081 atgtttgcca acgttggtgg aaatatatga ccgatgttat gcccgctaac ccggataaca
    34141 gcccggtgag tagcgagctg c
//
LOCUS       DAJVOY010000025        33917 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVOY010000025 DAJVOY010000000
VERSION     DAJVOY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.025"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            529..666
                     /locus_tag="OQL90_001048"
     CDS             529..666
                     /locus_tag="OQL90_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367903.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="OQL90_001049"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="OQL90_001049"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCS1367904.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2989..3918)
                     /gene="virB"
                     /locus_tag="OQL90_001050"
     CDS             complement(2989..3918)
                     /gene="virB"
                     /locus_tag="OQL90_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCS1367905.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4135..4371)
                     /locus_tag="OQL90_001051"
     CDS             complement(4135..4371)
                     /locus_tag="OQL90_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1367906.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4375..6276)
                     /gene="ipaA"
                     /locus_tag="OQL90_001052"
     CDS             complement(4375..6276)
                     /gene="ipaA"
                     /locus_tag="OQL90_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCS1367907.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDAVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6285..7283)
                     /gene="ipaD"
                     /locus_tag="OQL90_001053"
     CDS             complement(6285..7283)
                     /gene="ipaD"
                     /locus_tag="OQL90_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCS1367908.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7334..8425)
                     /gene="ipaC"
                     /locus_tag="OQL90_001054"
     CDS             complement(7334..8425)
                     /gene="ipaC"
                     /locus_tag="OQL90_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCS1367909.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(8445..10187)
                     /gene="ipaB"
                     /locus_tag="OQL90_001055"
     CDS             complement(8445..10187)
                     /gene="ipaB"
                     /locus_tag="OQL90_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCS1367910.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10193..10660)
                     /gene="ipgC"
                     /locus_tag="OQL90_001056"
     CDS             complement(10193..10660)
                     /gene="ipgC"
                     /locus_tag="OQL90_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCS1367911.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10718..11344)
                     /gene="ipgB1"
                     /locus_tag="OQL90_001057"
     CDS             complement(10718..11344)
                     /gene="ipgB1"
                     /locus_tag="OQL90_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCS1367912.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11360..11749)
                     /gene="ipgA"
                     /locus_tag="OQL90_001058"
     CDS             complement(11360..11749)
                     /gene="ipgA"
                     /locus_tag="OQL90_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCS1367913.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11762..13246)
                     /gene="icsB"
                     /locus_tag="OQL90_001059"
     CDS             complement(11762..13246)
                     /gene="icsB"
                     /locus_tag="OQL90_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCS1367914.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13560..15176
                     /locus_tag="OQL90_001060"
     CDS             13560..15176
                     /locus_tag="OQL90_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCS1367915.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15186..15548
                     /gene="ipgE"
                     /locus_tag="OQL90_001061"
     CDS             15186..15548
                     /gene="ipgE"
                     /locus_tag="OQL90_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCS1367916.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15548..16006
                     /gene="ipgF"
                     /locus_tag="OQL90_001062"
     CDS             15548..16006
                     /gene="ipgF"
                     /locus_tag="OQL90_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCS1367917.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16015..17130
                     /gene="mxiG"
                     /locus_tag="OQL90_001063"
     CDS             16015..17130
                     /gene="mxiG"
                     /locus_tag="OQL90_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCS1367918.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17138..17389
                     /locus_tag="OQL90_001064"
     CDS             17138..17389
                     /locus_tag="OQL90_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCS1367919.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17402..17695
                     /gene="mxiI"
                     /locus_tag="OQL90_001065"
     CDS             17402..17695
                     /gene="mxiI"
                     /locus_tag="OQL90_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCS1367920.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17701..18426
                     /gene="mxiJ"
                     /locus_tag="OQL90_001066"
     CDS             17701..18426
                     /gene="mxiJ"
                     /locus_tag="OQL90_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCS1367921.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18423..18950
                     /locus_tag="OQL90_001067"
     CDS             18423..18950
                     /locus_tag="OQL90_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCS1367922.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18892..19587
                     /gene="mxiN"
                     /locus_tag="OQL90_001068"
     CDS             18892..19587
                     /gene="mxiN"
                     /locus_tag="OQL90_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCS1367923.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19580..19987
                     /gene="mxiM"
                     /locus_tag="OQL90_001069"
     CDS             19580..19987
                     /gene="mxiM"
                     /locus_tag="OQL90_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCS1367924.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19971..20399
                     /gene="mxiM"
                     /locus_tag="OQL90_001070"
     CDS             19971..20399
                     /gene="mxiM"
                     /locus_tag="OQL90_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCS1367925.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20529..21161
                     /gene="mxiE"
                     /locus_tag="OQL90_001071"
     CDS             20529..21161
                     /gene="mxiE"
                     /locus_tag="OQL90_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCS1367926.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21148..22848
                     /gene="mxiD"
                     /locus_tag="OQL90_001072"
     CDS             21148..22848
                     /gene="mxiD"
                     /locus_tag="OQL90_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCS1367927.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22867..23934
                     /gene="mxiC"
                     /locus_tag="OQL90_001073"
     CDS             22867..23934
                     /gene="mxiC"
                     /locus_tag="OQL90_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCS1367928.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23947..26007
                     /gene="mxiA"
                     /locus_tag="OQL90_001074"
                     /gene_synonym="virH"
     CDS             23947..26007
                     /gene="mxiA"
                     /locus_tag="OQL90_001074"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCS1367929.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26020..26421
                     /gene="spa15"
                     /locus_tag="OQL90_001075"
                     /gene_synonym="spaK"
     CDS             26020..26421
                     /gene="spa15"
                     /locus_tag="OQL90_001075"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCS1367930.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26425..27717
                     /gene="spaL"
                     /locus_tag="OQL90_001076"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26425..27717
                     /gene="spaL"
                     /locus_tag="OQL90_001076"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCS1367931.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            27810..28148
                     /gene="spa13"
                     /locus_tag="OQL90_001077"
                     /gene_synonym="spaM"
     CDS             27810..28148
                     /gene="spa13"
                     /locus_tag="OQL90_001077"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCS1367932.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28135..29013
                     /gene="spa32"
                     /locus_tag="OQL90_001078"
     CDS             28135..29013
                     /gene="spa32"
                     /locus_tag="OQL90_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCS1367933.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29007..29888
                     /gene="spa33"
                     /locus_tag="OQL90_001079"
                     /gene_synonym="spaO"
     CDS             29007..29888
                     /gene="spa33"
                     /locus_tag="OQL90_001079"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCS1367934.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29893..30543
                     /gene="spa24"
                     /locus_tag="OQL90_001080"
                     /gene_synonym="spaP"
     CDS             29893..30543
                     /gene="spa24"
                     /locus_tag="OQL90_001080"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCS1367935.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30558..30818
                     /gene="spaQ"
                     /locus_tag="OQL90_001081"
     CDS             30558..30818
                     /gene="spaQ"
                     /locus_tag="OQL90_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCS1367936.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30818..31588
                     /gene="spa29"
                     /locus_tag="OQL90_001082"
                     /gene_synonym="spaR"
     CDS             30818..31588
                     /gene="spa29"
                     /locus_tag="OQL90_001082"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCS1367937.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31595..32623
                     /gene="spa40"
                     /locus_tag="OQL90_001083"
                     /gene_synonym="spaS"
     CDS             31595..32623
                     /gene="spa40"
                     /locus_tag="OQL90_001083"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCS1367938.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32636..32878
                     /locus_tag="OQL90_001084"
     CDS             32636..32878
                     /locus_tag="OQL90_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367939.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(33485..33917)
                     /locus_tag="OQL90_001085"
                     /pseudo
     CDS             complement(33485..33917)
                     /locus_tag="OQL90_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10563 a         4966 c         6796 g        11592 t
ORIGIN      
        1 ttgttgactg cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt aacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaatcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg cggtattaat tgctgatgct
     1081 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tgtaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggcgg gggcataaca
     2101 aatgatatta ctgagtacaa atttagctat attgggcgga ttagttgtgt gggaagacga
     2161 aatgtttctc ttgaaataag tgaactccct caaggaatga ttgtgtgctt aaatatttaa
     2221 gtgctggtaa gtttgagaag gctgacatat tttgcagagc aaacagtgat tgacttggat
     2281 gatattttta ttttagtcac tcgttgtaat tggagtatgc tttattttat ttgaaaatag
     2341 atgtatttta tacccgcaat ttaaactgta agccctatga ggggtatgga tattataagt
     2401 tactctttga ctaagcataa cttttggggt tagaaaaata aatctcttgt taataattaa
     2461 tatgatttta aaatcaaact tgtttataat tataaaatca tgtaagtcgg tcattttatg
     2521 ggaagattag gagaataaaa cagtaattat atttgagaaa tctgctaata tattcttaaa
     2581 gcttttttga tactgtgaac gaagtctgtt aatggtggat tttttgttta tcaccatgta
     2641 aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg aaacgattaa
     2701 aaatacgctg aatattttcg gccagtcact catcttcaca taaaattttg accatcatgc
     2761 cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat ttcaccagat
     2821 aatgttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa acacagtgcg
     2881 tggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt caagttcaac
     2941 gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt atgaagacga
     3001 tagatggcga gaaattatat ctcgaatagc ttcatctatt tctctctgaa ctgttttgtt
     3061 tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc tttttatgta
     3121 agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt tttgctttct
     3181 gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg ggggcaaatt
     3241 tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa gagattcatt
     3301 agccttttca agtcctttat aataattgaa taatatcttg tagtcattaa agttaagttc
     3361 tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct gaaaggcacg
     3421 agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg acatccctga
     3481 tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt ttgctgtttg
     3541 tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg aatatagaac
     3601 ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat ccagaatttc
     3661 aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct ttatagtttt
     3721 tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat tcgttttttg
     3781 gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga taaaggaaac
     3841 tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac ttaacaagtc
     3901 gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat tgacaaaagt
     3961 taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg tagtcttaaa
     4021 aattactatt ttaatagctg gattgatgca atttatcttt attggataaa agaataatat
     4081 tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca attactatat
     4141 tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa tgtgttctaa
     4201 tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca tttcaatcag
     4261 agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata gtttgctgta
     4321 cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca tattttaatc
     4381 cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg ctgctttata
     4441 tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata tatcatctgt
     4501 aagtaattct gcagaggtat cgtcaatttt tgacaatacc ttgctgaggg cgctagatac
     4561 ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt tggtttctgt
     4621 tacgtagttt attgtatttg catctgtaac ttttaatttg ttgtttattc tgtgttctgt
     4681 tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg ttcctgattt
     4741 agttccaatg agagatataa gattacttag ggagtttgtt actttttttg aagcttcaaa
     4801 tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag tctgaagtac
     4861 ttttgaatca gtgtttgagt tgaatttatt gatttgcgaa gctactctat gagaaagaga
     4921 agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc cttccgggtg
     4981 gagagggggg gtggttgaag agttctgtat gttcagaggc ctgtgtcccc gagaaagaga
     5041 gttatttgta gatctttctt gactactcgt agttaacagg tctgaaccta ttccatccga
     5101 attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa atgtctggac
     5161 agcatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg ctctttgtgg
     5221 atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag taggagactg
     5281 tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt caaaaactct
     5341 attatcgtaa gttctattat cattatttat atgatagtga attacaggtt gggatgtttt
     5401 ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg tagggaggcc
     5461 atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga cttttctcaa
     5521 cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta taccaaatcc
     5581 tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa gttttgcctg
     5641 tgcgtcaact aaatcatcta gtttgagaga tgaaacagtt tcattactta actttaattc
     5701 agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt tttgtcccaa
     5761 tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt gctcaatgtt
     5821 ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg cagaatcttg
     5881 ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt gaagtccctt
     5941 ttcgttttca aaattaagat cattttgatg tactaaggag tacaaaacat ttgaaaattc
     6001 gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc ttaacacatc
     6061 ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag acgttgcaaa
     6121 gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac tatggatatc
     6181 ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg ccttatataa
     6241 gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa atggagaaaa
     6301 agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac taaattatca
     6361 aaaatactat tggcattact gtatttttga actaaagttt gaagattatt tttcattgtt
     6421 tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc tagcacaacc
     6481 tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg ggtcatgttt
     6541 atattgacaa catatccccc gtttttttca gataccttgc cgattgttcc acctaattct
     6601 gtaagccatt tatttgcttg ttccttacta acagtattat ttgctggata tagcggttta
     6661 tctttatatt tttccttgag ctttgtcaat tcgtctttaa gcgatttgac ttgtaatttc
     6721 acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga aagaacagcg
     6781 ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac tttcagatac
     6841 tgttcattaa tattattgat tgactttgca attttatccc acagttctct gtgagatatc
     6901 atttcatatc catcaagctc ggcttctttc ggggctgaat gtaataattc tcttgcgtct
     6961 ttattaattg gatattcttt cttggaaaga atatccaata gttgagcgga tttgtttaca
     7021 tccatgctaa tctgagatga atgtaatgct atttcttcta gcgatgtttt agtcaacgtt
     7081 ttttgtgaga gatctttctt taatgcctga tttgttgttc tgatattatg aagggtgtcg
     7141 ttgagcatag taagggaact tacaggatga gaactggtcg ttgtttttat atcagaatta
     7201 actgtttcgg ttgatgaacc gttggtattg tttgggctga atgatgaggt ggaaatacta
     7261 ttagtcagag ttgttatatt catggttatt tccttatagg aagagccata tattattatg
     7321 gctcttggat atattaagct cgaatgttac cagcaatctg actggctgta gaattccttg
     7381 attggttgat gttgtcaatt atattcaata atttttgtat taattgattt gtcgcttggg
     7441 atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc tgactgatta
     7501 gttgttcttc ttcttcaaga gcagatgtat aacgccctcc tgatgtggat atattaccag
     7561 caacggctga tgtctccatt gttgcacggc caatgttttg tttttgctga gcagaaaggg
     7621 tattgagctc gtaagctctt ctctgggtgt caattttatc ctgcaaagaa atatcgggag
     7681 aactaagaga agttttatgt tccgttgaca gggatatatt atctggtgcc agtttatttt
     7741 tacctaaaat ttttgtgcta gagttagttt gtggtgaggt gatatttgta tctatttgtt
     7801 tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca gtggcaaggt
     7861 tctttcttaa ggctcctttt tgttcgctaa tccctgaatg cgtttttttg gcaccgatac
     7921 ccgttatacc tacttgtgtg accgctccag taatgcttga tgatagggct gctaggcctt
     7981 gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat gacaattgag
     8041 agcccaatct agtttctgca gtgcggagag cagaaagtag aacggctaca ctaataatta
     8101 aagaaacagc attagaagaa agactggaaa tatccagggt gttctctggg gtaattgtgt
     8161 gactcatttc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg atttctgaat
     8221 catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgta gttgttaata
     8281 cgggattttt accgacatta agtggaatat gtgctgctaa ctgctgataa ttttgtgatt
     8341 tttgtgtttc ggaagaactt tgagtttgtt ttgtagatat atctgtatat aaaatcgggg
     8401 ctgattttgt gttttgaatt tccataacat tctccttatt tgtatcaagc agtagtttgt
     8461 tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat tgaggctaac
     8521 agctctgcaa ttacttcctg caattggccg aatttttcta ttgcgtcacc gatgtatttt
     8581 gacagttgtt caacttgata tttcgataat gtcaggtctg ctagattttt cgacgcgttg
     8641 ttttggaaaa cagcagaagc gacatttcct cccgcttgtg tggcagagtt aacactttct
     8701 cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat tacttcatca
     8761 cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa atcgtccagt
     8821 tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt acctattatt
     8881 ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc tacgagaacg
     8941 actgctgcaa ccaagacaag agcgcctgcg attgccccca gaatagagcc aatcattttg
     9001 gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc tgaaaggagt
     9061 ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc catgaaggaa
     9121 ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc aacagctgcc
     9181 agtgctagag atgctcctcc agaaaaagct gctgcaacaa cactaacgat agttaaaagt
     9241 gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc tttacgtact
     9301 tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc ttggagagac
     9361 tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac tagttgaata
     9421 aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg ctgggttgat
     9481 agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc tttttcgagt
     9541 tgcattgatt tatctgtaag tttgctatga attgacagag ttttctgctc aatcaatgtc
     9601 ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg tcttaatttt
     9661 ttattttctg gtgaatctgg ggcgagttcg gataatcttg tttgaatttg gttaatttta
     9721 ttttctaggt cttttatttt tgaatctgcg ttttttagtt tattaatttg tttttcatag
     9781 tctctggtta gtccttcagt ttcagataga agagtattaa ttttatcgga gaattctagg
     9841 tttttttgct gtcttgcctg ttgctgggac ttccaggcag taattttatt tgttaatgca
     9901 gttaaagatt tttcaccaag tatttgaata aggtttccaa ttaaaagcgt tagttgggaa
     9961 cttgcattta atgactttgg tgctttaagt tcagggatta atatattcga agttgagtgt
    10021 gcattagttg tattaatatt tttgttagca gcaagatcag caattgtaag agaaaataat
    10081 ttgttagctg catcatttgc agcctggata gtattgtctc caagctcagt ggaagccaat
    10141 attttggcaa gaggcaaacc agtggttgtg gtgcttacat tatgcataat aattactcct
    10201 tgatatcctg aattgcgtct aagtatgatt gtgcttttat ttttaatttt tcatcattgc
    10261 tgtgttgaat tacgagttcg aagcactctt tggctttcaa gggggctttc aaccgaagct
    10321 ggcattgtcc agtatggaat accggtgtat agtcattttt tcctaatgca aaagcgacag
    10381 cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct gcgagtccca
    10441 taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa acttcagctt
    10501 cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca tccatcatat
    10561 catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca tcaattactg
    10621 cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct ttttgtctgc
    10681 attactttaa taagtataag atttaatata aacagattta atttgtattg ctttgacggt
    10741 atacagcctt tagaacctct gcgcttattt ctatttgtaa aacttttgtt gattcattaa
    10801 atattttatc gtagacggaa ctggatacta gatttgtaag atccttcatt tgtgtgttaa
    10861 acaaatccat gtcactgcta taacgtttta aaatctttgc tctgaggcca gatgaggcaa
    10921 tctcgtttag tgctgtatga ctgatagatg tttgtgctga tttttcatca agttttatat
    10981 ccgggaattt tttttcaata gctgcaaata tgtttttttt gttatctgag gtcaaaacaa
    11041 ctccattgtg tatggccatt tcatctatac ttctgtttat cattgaagag acataagagg
    11101 ttcgctcttg gctcatccaa caaaatagtt gattccccga attactatct tttattttgt
    11161 ctatgacgct gtccctgatt ttatttatat tcaccgggca tccaaaattc ttctgaggaa
    11221 agcgttgttt taagttaaaa acagataata ttttctttac aagcttattg tgacttaacg
    11281 aatcaaatga tgggcgagga atcgcgaatt caacctgtgg aagtattttg tttagaattt
    11341 gcatgatacc ccctatatgt tagttcactt ctgaagtgat gtttgcaatg gttagtttaa
    11401 tacaaaataa aaattcgtac aaagattcaa tgagttcatc aggtgaaaga tattttttgt
    11461 agtgtttctt ggtcaaaaca cattgatgag tatcagggag aagacagata gaagaaccat
    11521 aatctctact caaagagtag ttaaggatga gtatgtgttc tggattaata atattttctg
    11581 taaaaagttc atgttcatta agaagtccgg tcaaaaagat gtattcatca ttatctgtta
    11641 tacaaacagg tatttgttct tcgagcaata taaaggcttg atttttatcg ttaaaatcaa
    11701 gctttgcacc tgttatttca taaagtttat catatagttt gcgacacatt tctatggcct
    11761 tctatatatt agaatgagag ttattcaata aaagcttatt gacctgtaat aagtcgtgaa
    11821 tgtaaagttt ggttccaaga tctggcgatt taagagaatt gtaataatca atagttttta
    11881 ttgacttttc gataattact tcttttttat ctgttgctcc ggtttttaat tcgtttaata
    11941 cacgagacat attgctgggt gctgatttta ctaagtatga gtggcaagcg ttgatgaccg
    12001 catcaaagtc ttctgaagat acattttcag taggatgttt gtaaagtgaa attgataaag
    12061 gcttgaatgt ttttggggtg tggataaggt taattttatt gaaactttta agatagctac
    12121 gtcgtaattg agtcaatctg gaaagatcaa aatcagattc aatatacttc attatctcgt
    12181 tataagactg attgagactt tcaatgcgtt gcctaacttt atttgcatac gcgtatacat
    12241 tgttaggtgt agcaaccaat ttaacgtcag ggagtggaaa gtatatttca gaatttcctg
    12301 ctttaagaac attaagtgcc ataccagcac agttttcaga tttactgatt aatttataat
    12361 ttgccaattg tctgaaagca tctgctttct tttggcatat aaattttgac atgtttgttt
    12421 catcaaggcc aaaaaggtta tagctgattt taccatcctt tgttttatta tcacctgata
    12481 atggaatgta tactttatca gcactgaccc cccaataacc ataggggtct tttttttgat
    12541 tagctctggg aaaaaatatt tttttgttgt tattttgatc ttgttttgaa tttaataagt
    12601 ttctaacctc ttggcctgca tcaagtcttt cggctgtttg gtctgaaatc atatttagtt
    12661 tgtcttgttt ataggtatcg acactgtaac ctgagcgttt ttcaaaaagg tagtttattt
    12721 ctgtatcttt tgtaacattc ccgccgggat accagctgag ataggtatga gttggttcct
    12781 gtagtaaatt gttttttata ctgatactgg tatgccctaa atgggttatg cctctatttc
    12841 cccagcaacg aaacactaaa tgtctttcaa gatttttggc tgtaccgatg ccatgaaaac
    12901 tctctgcggt attcaacatt aatttgatgt ctttacctgt aaaagaatgt tgaattgaat
    12961 ttttgactgc ttcataggcg gcaaacgcac tttctgcaga tttttcgcta gctaatgtct
    13021 tgaggaaaag caataaggat ttttgattcg ctagtcgata attttttagt tgttcattta
    13081 atatctgtga atttatcttt gcattaatag tgcttaatgt tctgaaaaag agatccttta
    13141 ggttgaattt ctgtgctacc aatataggtc catgctctgt atcttcaacg cgtataggcc
    13201 cttttgtatt gcttgcgtca atgaaattgc taattttgag aatcatactt tattaactct
    13261 ccattacgtg gtgattgtgg ggatgtaaaa tgtatttgat atataatata ttcaattaat
    13321 aaatttagaa acttgagcct gttaacataa tcaaattttc ttttgctgta cataatatgt
    13381 acctcgtgag catatgtagt gctggtttca tcatgaaatc ccacaagtta aagtgtctga
    13441 tatatcaggc tcggagtgtt atagaaaaag agagaaccct gttgaataag tatctttttt
    13501 gttgtaaaac gaggtacaaa actgcaatca atgccccatt tatgactgag gataattaaa
    13561 tgcacataac taatttggga ttgcatcagg tttcatttca aagcggagat tcctataaag
    13621 gcgcagaaga aaccggaaac caaaaaggtg taagcgtgat ttcatatcaa agagttaaaa
    13681 atggagagag aaataagggg gtcgaagcgc ttaatcgatt atatttacag aaccaaacaa
    13741 gcttaacagg gaaaggcctt ttgtttgcgc gtgacagagc tgcagttttt tacgaggcaa
    13801 taaagttggc aggtggtgat acgtcaaaaa ttaaagccat gatggaacaa ttagatacct
    13861 ataaacttgg tgaagttgat aaaagaaata ttaatgagct taataaagta ataagtgaag
    13921 agataagagc acagctaggc attaaaaata aaaaggaatt gcaatcggaa ataaaacaaa
    13981 tatttactaa ttatttaaat aataagaatt gggaaccgat caacaaaaat attaattata
    14041 atgggaaaaa ttatggtttt caattaacac ccgcctctca tatgaagata gggaataaaa
    14101 atatatttgt caaagagtat aatggaaaag gaatttgttg tgcttctacg agagagagta
    14161 agcatattgc gaatatgtgg ctttcaaaag tggtggatga cgaggggaag gaaatttttt
    14221 cgggtatcag acatggagtt atatcagcct atggattgaa aaaaaactca tctgaaagag
    14281 ctgttgcagc tcgtaataaa gctgaggagt tagcaagcgc agcattatat agcagacaag
    14341 aattattatc acaggctttg tctggtaaaa cagtagattt aaagattgtt tcgacttctc
    14401 ttctgacgcc gaccagttta actgggggag aggagagtat gttaaaagat caggtaaagg
    14461 cattaaaaga gttaaactct aaaagggggg aaccaacgaa attattaatt cggaatagtg
    14521 atggtcttct gcaagaagta aaggttaatc taaaagtggt gacatttaac tttggtgtaa
    14581 atgaattagc gctaaaaatg ggcttaggct ggaggaatgt tgacaagctt aatggtgaat
    14641 caatatgttc tttgttgggg gataatttcc tcaaaaatgg tgtgattgga ggttgggctg
    14701 cagaagctat agaaaaaaat ccaccatgta agaatgatgt gatatatctg gctaaccaga
    14761 taaaagagat tctaaacaag aagttacaaa aaaatgataa tggagagccg tataaattgt
    14821 cacaaagaat ggctctattg gcttatacta taggtgctgt accttgctgg aattgtaaga
    14881 gtgggaagga cagaacaggt atgcaggatg ctgaaattaa aagagaaata ataagaaaac
    14941 atgaaacagg gcagttttct caattaaata gtaaattatc ctcagaagag aaaagattat
    15001 tttctactat tctaatgaat agtggtaata tggaaatcca agagatgaat actggtgtgc
    15061 ctggaaataa agttatgaaa aaattaccgc tatcttcgtt agagctgtct tatgctgaaa
    15121 gaatagggga ccccaaaata tggaatatgg tgaaagggta ttcgtcattt gtataagagg
    15181 aatatatgga agatttagca gatgttattt gccgagcctt gggtatccct tcaattgata
    15241 ccgatgatca agcaatcatg cttgatgatg atgtgttaat atatattgaa aaagaaggag
    15301 attcaataaa tttattatgt cctttttgtg cattgcctga aaatataaat gatcttatat
    15361 atgcgttgag cctaaattac tcagaaaaga tatgtttagc tactgatgat gaaggtggaa
    15421 gtctaatcgc gcgattagat cttaccggga taaatgaatt cgaggatata tatgttaata
    15481 cagaatatta tatttcgcgt gtgcgttggc tgaaggatga atttgcgcga agaatgaagg
    15541 ggtattaatg tcccgtttcg tatttatttt tctgtgtttt atcccatatt tagggagagc
    15601 tgattgttgg gataaggctg gtgaaaggta caacattcca tcgagtttgt taaaagcgat
    15661 tgcggaaaaa gaatccggat ttaataaatt tgcagtcaat gttaataaca atggaagtaa
    15721 agattatggt ataatgcaaa taaatgactt tcattctaaa agacttagag aaatgggata
    15781 tactgaggaa atgctaatta gtcatccatg tctttctgta cattatgcag caaagttatt
    15841 aaatgagttt atgctgatgt atggaagagg gtgggaggct gttggtgcct ataatgcagg
    15901 aacatctccg aaaaagaaaa aagaaagatt aaagtacgca gaggatatct ataaacgcta
    15961 tttgcggata gcagcagaga gcaagcagaa taatcgaagg atataagagg atttatgtct
    16021 gaggcaaaga actcaaatct tgcaccattc aggttattag tgaaactcac aaacggtgtc
    16081 ggggatgagt ttccgttata ttatgggaat aacttaattg ttctcggacg gacgatagaa
    16141 acacttgagt ttggaaacga taatttccca gaaaatatta ttccagtaac agatagtaaa
    16201 tcggatggta taatttattt ggccataagt aaagataata tctgtcaatt tagtgatgaa
    16261 aaaggtgaac aaattgatat caattcacaa tttaactcat ttgaatatga cggagtttct
    16321 tttcaactta aaaatatgat ggaggataaa tcgagagttc atatactaaa cggaatgtat
    16381 aataaaccct cggtgttttt tttctttgct gttattgttg tgttaattat aattttttcc
    16441 ctctctttaa agaaagatga agtaaaagaa attgctgaaa ttatagatga taagagatat
    16501 ggaatagtca acacaggaca gtgcaattat attcttgccg aaacacaaaa tgatgcggta
    16561 tgggcatctg tagccttaaa taaaagcgga tttaccaaat gcagatatat tctggtgtcc
    16621 aacaaagaaa taaatagaat tcaacaatat atcaatcaac gattcccata tatcaatttg
    16681 tatgtgttga atttagtgag tgataaagcg gaattactgg tatttttaag taaagaaaga
    16741 aatagttcaa aagataccga acttgagaaa ttgaaaaatg ctctgatagt tgaatttccg
    16801 tatatcaaaa atattaaatt caactatctt agcgatcata atgcaaggga ggatgctaaa
    16861 ggtattttca caaaggtaaa tgtccagtat aaagagattt gtgaaactaa taaagttaca
    16921 tattctgtca gagaagaact gacagatgaa aagcttgagc tcattaatag actgatttct
    16981 gatcataaaa atgtatatgg agatcagtat attgagtttt ctgtactttt gatagatgat
    17041 gattttaaag gtaaatcata tcttaacagc aaagacagtt atgtaatgtt gaatgataaa
    17101 cactggtttt ttttagataa aaataagtga ggataaaatg agtgttacag taccggataa
    17161 agattggact ctgagttcat tatctgaaac ttttgatgat ggaactcaaa cattacaagg
    17221 gcaactaaca tcggcactaa atgcattagc tgaaaatcct tcgaatccac agttgctggc
    17281 tgaataccaa agtaaattat ctgaatatac attatatagg aacgcgcaat ctaatactgt
    17341 gaaagtgatt aaggatgttg atgctgcaat tattcaaaac ttcagataat agggagcatt
    17401 catgaattac atttatccag tcaatcaggt tgatattatc aaagccagtg attttcaatc
    17461 tcaagagata tcaagcctgg aagacgtcgt gtcggctaaa tatagtgata ttaagatgga
    17521 tacagatatt caagtatcac aaataatgga gatggtaagc catccagaat cattaaaccc
    17581 agaatctttg gccaagttac agacgacgct ctcaaattat tcaataggag tatcattagc
    17641 tggcacgtta gcaagaaaaa cagtttcggc tgttgaaact ttattaaagt cttaatttat
    17701 atgattaggt ataaatgttt tattttattc ttgttgctga tgttgattgg atgtgagcaa
    17761 cgtgaagagt taatttctaa tttatctcaa agacaggcaa atgaaataat atctgtgcta
    17821 gaacgccata atattactgc tagaaaagtt gatggaggta aacaggggat ctcggtacaa
    17881 gtcgaaaagg ggacatttgc atcggcagtt gatttgatgc gcatgtacga tttgccaaat
    17941 ccggagagag ttgatatctc acaaatgttt cctacagatt cattagtgtc ttctccaaga
    18001 gctgaaaagg cccgtttata tagtgctatt gagcaacggc tggaacagtc tttagtttct
    18061 attggtggtg ttatttcggc aaaaatacat gttagctatg atcttgaaga aaaaaatata
    18121 tcttcaaaac cgatgcatat atcagtaatc gctatatatg actcaccgaa agagtctgaa
    18181 ctattagtta gtaatattaa gcgatttttg aaaaacacct tttctgatgt taagtatgaa
    18241 aatatatctg tcatattaac tccgaaagaa gaatatgttt atacaaatgt acaacctgtt
    18301 aaggaaatta aatcggaatt tttaacaaat gaagtaatat atgtatttct cgggatggct
    18361 gtactagttg tcattctttt ggtatgggca ttcaaaacag ggtggttcaa gagaaacaaa
    18421 atatgataag aatggatgga atttataaaa aatatctttc aataattttt gatccagcgt
    18481 tctatataaa tagaaatcgg ttaaatttgc cttctgaact gttagaaaat ggcgtaatca
    18541 gaagtgagat taataatctc ataattaaca aatatgattt aaattgcgat attgaacctt
    18601 taagcggggt aaccgctatg tttgttgcca actggaattt acttccagct gttgcttatt
    18661 ttatcggtag tcaagagtct cggttaatta atcatagtga gatggttatt agttattatg
    18721 ggggcaaaat cagtaagcaa ggggaggccg ctattcgaag tggtttttgg catcttattg
    18781 agtggaagga aaatatatct gttggtatat atgaaagaat aaacttatta tttaatccaa
    18841 ttgctttgga gggaaattat acgcctgtag aaagaaattt gtcccgatta aatgaaggta
    18901 tgcaatatgc aaaaaggcac tttaccggta tccagacatc atgcctatga tggtgttgtt
    18961 ataaagagaa ttgaaaagga attatgtaaa accataaaag atagagacac tgagagtaag
    19021 aagaaagcaa tttgtgttat aaaggatgct acgaaaaaag ctgaatcact gcgtatagat
    19081 gctgtatgcg atggttatca aattggtata cagactgctt ttgagcatat tattgactat
    19141 atttgtgaat ggaagttaaa gcaaaatgaa aacagaagaa atattgagga ctatataact
    19201 agtttgcttt cagaaaattt acatgatgag aggatcatta gcactttatt ggagcagtgg
    19261 cttagttcgt tgcgtaatac agtaacagaa ttaaaagtgg ttttgcccaa gtgtaattta
    19321 gcactgagga aaaagttaga acttgatctc cataaatatc gctcagatgt caagattatt
    19381 ttaaaatatt ctgaagggaa taattatata ttctgtagtg gtaatcaagt agtggagttt
    19441 agtcctcagg atgtaatatc aggtgttaaa atagaacttg cggaaaaatt aacaaaaaat
    19501 gataaaaaat atttcaaaga gttagcacat aagaaactga gacaaattgc agaagacttg
    19561 ttaaaagaga atcctgttaa tgattaatca aataaatgca agcaatgctc tgcagcaaag
    19621 attaaatagt gaagaatttg ttaatttaaa tgagagatta tcatcttcgc aatcatttga
    19681 tgagaatata atctatgaaa taatgcaata tttctcacag agtgaattaa attccataga
    19741 taatgatgaa ctgcacaata agattgaaca attattcaat tcacgttttc cttatcttac
    19801 agctgcacaa aaaagttcac ttcttaataa actcatagat gcaaatcaat atgtcgattt
    19861 gcatgaggga ttttatgcat cattgtccat atataacaat attgatttct atattaagac
    19921 tacaaccttc gattcattaa ttagtgtctt tgaagcaggg agagaggcag atgattcgac
    19981 atggtagtaa taagttgaaa atatttattt taagtatatt gctattaaca ctgagtgggt
    20041 gtgctttaaa gtcatcatct aattctgaaa aagaatggca tattgttcct gtaagtaagg
    20101 attatttttc tattccaaat gatttattat ggtcgtttaa tacaaccaat aaaagtataa
    20161 atgtttactc taaatgtatt agtggtaagg cggtttatag ttttaatgca ggtaaattca
    20221 tgggcaactt taatgttaag gaagtagatg ggtgcttcat ggatgcacaa aagatagcta
    20281 tagataaact attttctatg ctgaaagacg gggttgtttt aaaaggtaat aagataaatg
    20341 ataccatcct tatagagaag gatggggaag ttaaattaaa attaattcga gggatataat
    20401 tgtattgtga gtaaatataa aggtctaaat acaagtaata tgttttacat ttactctagt
    20461 ggacatgaac cagttaacgt tgagcttgta aaagataaag aacgtaacat aattgagctg
    20521 gctccagcat ggaagggttt ttttttgtcc gaaatcaaaa tataaaattc agtgataatg
    20581 tgaactatca ttatcgcttt aacattaata gttgtgcaaa atttcttgct ttttgggact
    20641 atttcagtgg tgctctagta gagcatagcc atgctgaaaa atgtattcat ttctatcatg
    20701 agaatgatct tcgggatagt tgtaataccg aatcgatgct tgataagtta atgttaagat
    20761 tcatcttttc atctgaccag aatgtaagta atgcactggc tatgatacga atgactgagt
    20821 catatcattt ggttctttat cttctgagaa ctattgagaa agaaaaggag gtaagaataa
    20881 agagtctgac agagcattat ggtgtgtcgg aggcttattt tagatcttta tgtagaaaag
    20941 ctttaggggc aaaagtcaaa gaacaactga acacttggcg gttagtaaat ggtttattag
    21001 atgtattttt gcacaatcaa acaatcactt ctgctgccat gaataatggg tatgcatcaa
    21061 catcccattt ttcaaatgag ataaaaacac gactaggctt ttctgcaaga gaattatcga
    21121 atattacttt tttagtgaaa aaaataaatg aaaaaattta atattaaatc tttgactctc
    21181 ttgattgtat tgttaccatt gatagttaat gctaataata ttgactctca tttattagaa
    21241 caaaatgaca tagcgaaata tgttgctcaa agtgatacgg taggatcttt ttttgaacgt
    21301 ttttctgcgt tattaaatta tcctattgtt gttagcaaac aagctgcaaa aaagagaata
    21361 tctggggagt ttgatttatc taatccagaa gaaatgcttg aaaaaatgac attacttgtg
    21421 ggactcatat ggtataaaga tggaaatgca ttatacatat atgactctgg agaattaata
    21481 agcaaagtta ttttgctgga gaatatatca cttaattatc ttattcagta tctgaaggat
    21541 gctaaccttt atgatcacag atatcctata agaggaaaca ttagtgataa aacattctat
    21601 atatctggtc ctccggcatt ggttgaactg gtagctaata cagcaacttt gcttgataag
    21661 caggtttctt ctattgggac agataaagtt aattttgggg ttataaaatt aaaaaataca
    21721 tttgttagtg acagaactta taatatgaga ggggaggata ttgtcatccc aggggtggct
    21781 actgttgtag aacgtttatt aaataatggt aaggctctga gtaacagaca agccaaaaat
    21841 gatccgatgc caccctttaa tattacacag aaagtatctg aggatagtaa tgacttttca
    21901 tttagttcag ttaccaatag ttctatatta gaggatgtat cacttattgc atatccagaa
    21961 accaattcta tattagtcaa aggtaatgat cagcaaatac agattattcg cgatattatc
    22021 actcagttag atgtagccaa acgacatata gaattatcat tatggataat cgatattgat
    22081 aaatctgaat taaataacct tggcgttaat tggcaaggaa cggcttcatt tggtgattcg
    22141 tttggtgcat catttaatat gagctcatca gcatcgataa gcactttgga cggcaataag
    22201 tttattgctt cagttatggc attaaatcag aaaaagaaag caaatgtagt ttcaaggccg
    22261 gtgattttga cacaagagaa tattccggct atatttgata ataacaggac tttttatgtg
    22321 tcattagttg gtgagcgcaa ttcttcgttg gagcatgtta cgtatggaac attgataaat
    22381 gttatacctc gcttttcatc tcagggacaa attgagatgt ctttaacgat agaggatgga
    22441 acaggaaata gtcagtcaaa ttacaattat aataatgaaa atacatctgt ccttccagaa
    22501 gttggaagga ccaaaatcag tacaatagcg agagttccac aaggcaaaag tttgttgata
    22561 ggtggttata cgcatgaaac gaactctaac gaaatagtat ctattccttt tctctcctct
    22621 attcctgtaa tcggtaatgt gttcaagtat aaaacaagga atatcagtaa tattgtaagg
    22681 gtttttctga ttcaaccccg tgagataaag gagtcaagtt attataatac tgcagaatat
    22741 aagagcctca tatctgaaag agaaatacaa aagactacac aaataatccc atctgaaact
    22801 actttgctgg aagacgaaaa atcattggtt tcatacttaa attactaact ataaagtagg
    22861 tgatgtatgc ttgatgttaa aaatacagga gtttttagct ctgcattcat tgataagttg
    22921 aatgcaatga caaattcaga tgatggagat gagactgctg atgcagagct tgattctggc
    22981 ttggctaata gcaagtatat tgactcatct gatgagatgg cttccgctct ttcgtcattt
    23041 ataaacagaa gagaccttga gaaactgaaa ggaacaaata gtgatagtca ggaacgtatt
    23101 ttagatgggg aagaagatga aattaatcac aagatttttg atttaaagag aactttaaaa
    23161 gataaccttc ctctagatca ggattttata gacagactaa agagatattt taaagatcca
    23221 agtgatcaag tcttagcatt aagggaactt ttgaatgaaa aagatcttac tgctgaacaa
    23281 gtcgaatcat taactaaaat tattaatgag ataatatcag gtagtgaaaa aagtgttaat
    23341 gctggaataa attcagctat acaggctaaa ttatttggca acaaaatgaa acttgaacca
    23401 cagcttttgc gtgcatgtta tcgtggtttt atcatgggga acatatcaac aacagatcag
    23461 tatatagaat ggcttggtaa ttttggtttt aatcacagac atacaattgt gaattttgta
    23521 gagcagtcac tgattgtaga catggattct gagaaaccga gctgtaatgc ttatgagttt
    23581 ggttttgtgt tatctaaatt aattgcaatt aagatgatta gaacttcaga tgtaattttt
    23641 atgaagaaac tggaatcctc aagcttgcta aaagatggca gtttaagtgc agagcagcta
    23701 ttgctaactt tattatatat ttttcaatat ccaagtgaaa gtgagcaaat tcttacttct
    23761 gttatagacg tatcacgagc cagtcatgag gattctgtag tgtatcaaac atatctatct
    23821 tctgttaatg aaagtcctca tgatatattt aaaagtgaaa gtgaaagaga aattgcgatc
    23881 aatattctac gagagcttgt cacaagtgca tacaagaaag agctttctag ataacaggag
    23941 atgaaagtga tccagtcttt tcttaagcaa gtaagtacta agcctgaatt aattatatta
    24001 gtgcttatgg ttatgatcat tgctatgttg atcataccat tacctacata ccttgttgat
    24061 tttttaatag ggcttaatat tgtacttgct attctcgttt ttatgggatc attttatatt
    24121 gaaagaatat tgagtttttc tacattccct tcggttttgt tgataacgac attatttcgt
    24181 ctcgctttat caataagtac tagtcgacta attttagttg atgcggatgc gggtaaaatt
    24241 attactacat ttggccagtt tgtcattggt gatagtctgg ctgtaggttt tgttattttt
    24301 tcaatagtaa ctgttgtgca gttcattgtt ataacaaaag gatctgaaag ggttgcggag
    24361 gtagctgctc gcttttctct tgacggtatg ccggggaaac aaatgagcat tgatgcggat
    24421 ttaaaggctg gaattattga tgccgcagga gctaaagaaa gacgcagtat tctggaacgt
    24481 gagagtcaat tgtatggttc atttgatgga gcaatgaagt tcattaaagg tgatgcaatt
    24541 gctggcatca ttattatttt tgtcaattta ataggaggta tttctgttgg tatgagtcaa
    24601 catggaatgt ctctatctgg tgcattatct acttatacca tactaactat tggtgacgga
    24661 ttggtctctc agattcctgc tctgttaatt tctataagtg caggatttat cgttacaaga
    24721 gttaatggtg atagcgataa tatgggacgt aacataatgt cccagatatt tgggaatcct
    24781 tttgttctca ttgttacatc agctcttgcc ttggcgatag ggatgttgcc aggttttccg
    24841 ttttttgttt ttttcctgat agcagttact ttgacggctt tattctatta taaaaaggtc
    24901 gtagaaaaag aaaaaagtct gtctgagtct gattctagtg gctatactgg tacatttgat
    24961 attgataata gccatgactc atctttggca atgatagaaa atctggatgc tattagttca
    25021 gaaaccgttc ctttaatttt attatttgcc gaaaataaga taagtgcaaa tgatatggaa
    25081 ggtcttattg aaaggataag aagtcagttc tttattgatt atggtgtcag gcttccaact
    25141 attttatata gaacaagcaa tgagcttaag gtcgatgata ttgttttgtt aataaatgag
    25201 gtgcgtgccg atagttttaa tatatacttt gacaaagtgt gtattacaga tgaaaatgga
    25261 gatatagatg ctctaggtat tcctgttgtt tcaacttcat ataatgaacg tgttatttct
    25321 tgggtagacg tttcatatac agaaaatcta actaatattg atgctaaaat taaaagtgct
    25381 caagatgaat tttatcacca gttgtcacaa gctttattaa acaacataaa tgagattttt
    25441 ggtatacaag aaacaaaaaa tatgttagat cagtttgaaa atcggtatcc tgatctatta
    25501 aaggaagtct tccgacatgt gactatacag agaatttctg aggtattaca aagattgctt
    25561 ggagaaaata tttctgttcg caatttaaaa cttattatgg agtctttggc gctttgggct
    25621 cccagagaaa aagatgtcat aacattagtt gaacatgtcc gtgcatcact ttctaggtat
    25681 atttgtagta aaatagctgt ttctggtgag attaaagttg tgatgctttc cggatatatt
    25741 gaggatgcaa taagaaaagg gataaggcaa acctctggtg gctctttctt gaatatggat
    25801 atagaggttt cggatgaggt aatggaaact ttagcacatg ctttgagaga attgagaaat
    25861 gcaaaaaaaa atttcgttct tttggtatca gtagatatac gtaggtttgt taaaagactt
    25921 atagataaca gatttaagag tatactcgtt atatcgtatg ctgagattga tgaagcatat
    25981 accattaatg tattaaagac tatttagtga ggtttaaata tgagtaacat taatttagtt
    26041 caattagtta gagatagtct tttcacgatt ggttgtcctc catcaattat tacagattta
    26101 gacagccatt cagcgattac catttctttg gatagtatgc ctgctattaa tatagctctt
    26161 gtcaatgagc aggttatgtt atgggctaat ttcgatgcac caagtgatgt aaaacttcag
    26221 tcatctgctt ataacatatt aaatttaatg ttaatgaact ttagttatag cattaatgag
    26281 ctggtagagc ttcatcgttc tgatgagtac ttacagttaa gagttgttat taaagatgac
    26341 tacgttcacg atgggattgt ttttgcagag attctgcatg agttttatca gaggatggaa
    26401 atcttaaatg aggtcttata atcaatgagc tatataaaat tgctcactca attatctttt
    26461 cctaatagaa tctcggggcc aatcttggaa acaagtctta gcgatgtttc gattggtgag
    26521 atttgtaaca ttcaggctgg aattgaaagt aatgaaatta ttgcaagagc tcaggttgta
    26581 ggatttcatg atgaaaaaac aatattaagc ttgattggaa attctcgtgg actttcaagg
    26641 caaacgttga ttaagcccac tgcccagttt cttcatacgc aagtcggccg tggattattg
    26701 ggagcagtag tcaatccttt aggggaggtt actgataagt ttgctgttac ggataacagt
    26761 gaaattcttt atcgacctgt agataatgct cctccgctat atagtgaaag ggctgcaatt
    26821 gagaagcctt ttttaacagg tgttaaggtt attgattctt tactcacgtg tggtgaagga
    26881 cagcgaatgg ggatttttgc gtcagctggt tgtggcaaaa cttttctcat gaatatgctc
    26941 attgaacata gtggtgctga tatatatgtt attgggttaa ttggtgagcg aggtcgagag
    27001 gttacagaaa cggttgatta tttgaagaac tctgagaaaa aaagcaggtg tgttttagta
    27061 tatgcaactt cggattactc ttcggttgat cgttgtaatg ctgcatatat agccactgct
    27121 atagccgaat tttttaggac tgaaggacat aaagtagcgc tttttattga ttcattaaca
    27181 aggtatgcca gagcattacg tgatgtggcc ttagccgctg gagaatcacc tgccagaaga
    27241 ggctatccgg tttcggtttt tgatagctta cccagacttc ttgaaaggcc aggaaagtta
    27301 aaggcaggtg gctctattac tgcattttac actgttcttt tggaggatga tgattttgct
    27361 gatccattag ctgaagaggt aagatccatt ttagatggac atatatattt gagcagaaat
    27421 ctagcccaaa aaggacaatt tcctgcaatt gattccttaa aaagtataag cagggtattt
    27481 acacaggttg ttgatgaaaa acatcgtatt atggccactg catttcggga gctactttct
    27541 gaaatagaag agctaaggac aattattgac tttggtgaat acaaaccggg ggagaatgcc
    27601 tctcaggata aaatctacaa caaaatatct gttgttgaga gttttctgaa gcaagattat
    27661 cgactcggtt ttacttatga gcagacaatg gagcttcttg gtgaaacaat tagataaggt
    27721 tttaaaaatc aaagataaat atcaacggtc tgttaaatta attgaagcac acatattaac
    27781 actaaaaaaa aaactctttg tatcgagatg tggaggcatt agataaaagg attatatact
    27841 tcctgcaact ggagaatgac ttggaacctg taggcgctca gagtgttagt cagcttttca
    27901 atacaagacg taagatcgct atagtaaaaa agcatattat ccaatatcag tctgaacgaa
    27961 ttcttctaaa gggaagaatt gaagaaattc aaaaagatat tgatgaggca aatgcttcta
    28021 aaagaaaact gcttcataaa gagagtaaaa tatgtaaacg gattggcttg attaaaagaa
    28081 ataactttgc caaacagttg atacttgatg agttatcaca agaggatatg aagtatggca
    28141 ttagataata taaacctaaa ttttagtagt gacaaacaga tagaaaaatg tgagaaacta
    28201 tcttcaatag ataatattga tagtctcgtt ttgaagaaga agaggaaggt tgaaattcct
    28261 gagtactctt taattgcatc taactatttt actattgata agcactttga acataagcat
    28321 gataaaggag aaatttatag tggcattaaa aatgcgttcg aacttagaaa cgaacgagcg
    28381 acatattctg atattccaga atcaatggcc attaaagaaa atattttgat accagatcaa
    28441 gatatcaaag caagggaaaa aataaatatc ggcgatatga gggggatctt ttcatataat
    28501 aagagtggaa atgcagacaa gaacttcgaa agaagtcata cttcttctgt aaaccctgat
    28561 aatctgctag aatctgataa tagaaatggt caaattggtt taaaaaatca tagcttgtct
    28621 attgataaga atattgctga catcatttct ttactaaatg gaagtgttgc taaatcattt
    28681 gagctgcctg taatgaataa aaatactgca gacataaccc catccatgtc attgcaagaa
    28741 aaatcaatag ttgaaaatga taaaaatgtt tttcaaaaaa atagtgaaat gacttaccac
    28801 tttaaacagt ggggggctgg acattctgtt agtatttcag tggagtctgg ttcttttgtt
    28861 ctaaaaccgt cagatcaatt tgtaggaaat aaacttgact taattttgaa acaagatgct
    28921 gagggtaatt acagatttga tagcagtcaa cataataagg ggaataaaaa taatagtaca
    28981 ggatataatg aacagagtga agaagaatgc taagaattaa acattttgac gctaacgaaa
    29041 aactacagat tttatatgca aagcaactct gcgagcgttt ttcgattcag acattcaaaa
    29101 ataaatttac aggcagtgaa agtttagtca ctcttacttc cgtgtgtggg gattgggtaa
    29161 ttcgtattga tacattatct tttttgaaaa aaaaatacga ggtattttca ggattttcta
    29221 cacaagaatc tttactgcat ttatcaaaat gtgtctttat agagtcgtca tctgtatttt
    29281 cgattccaga actgtctgat aagattactt tccggatcac gaatgaaatc cagtatgcaa
    29341 ctactgggag tcatttatgc tgtttttcct cttctttagg tattatttat tttgacaaga
    29401 tgccggtatt acgtaatcaa gtttctcttg acttattgca tcatctttta gagttttgct
    29461 taggttcatc taatgtaagg ctggctactt taaaaagaat tcgtactggt gatataatca
    29521 tagttcagaa actttataat ttattattgt gtaatcaagt tattattggg gattatattg
    29581 tgaatgataa taatgaggca aaaattaatc tgtcagaaag taatggtgag tcagatcaca
    29641 cagaagtttc tttggcatta ttcaattatg atgatatcaa tgtaaaagtg gactttatcc
    29701 ttttagaaaa aaagatgaca atcaatgaac taaaaatgta tgtagaaaac gaattattca
    29761 agtttcccga tgacatagtt aaacatgtaa atattaaagt aaatggttct ttggttgggc
    29821 atggggaact tgtttctatt gaggatggtt atggtatcga gattagttct tggatggtaa
    29881 aggagtaatc tcatgctgag tgatatgtcc ctcatcgcta cactttcttt ttttacgcta
    29941 ttaccatttt tagttgctgc tggtacttgt tatataaagt tttctattgt ttttgtaatg
    30001 gtacgaaatg cacttggttt gcagcaggtt ccatcaaata tgactctcaa tggtattgca
    30061 ttgattatgg ctttgttcgt gatgaaaccc attattgaag ctggttatga aaattatcta
    30121 aatggcccgc aaaaatttga tacaatatca gatattgtcc gtttttctga ttctggcttg
    30181 attgaataca agcaatactt gaaaaagcat acagatcttg agttggcacg ttttttccaa
    30241 aggtctgaag aagaaaatgc tgatttaaag agtgcagaga ataatgatta ttcattgttt
    30301 tccctcttgc ctgcatatgc gctaagtgaa attaaagatg cgttcaaaat aggtttttat
    30361 ttatatttgc catttattgt tgttgatctt gttatttcaa gcattttact tgcacttggt
    30421 atgatgatga tgagtccaat cacaatatca gtaccaataa aacttgtatt atttgttgcg
    30481 cttgatgggt gggggatcct gtctaaggcc ttgattgaac aatatatcaa tgttcctgct
    30541 tagatgatgg aggtaatgtg tctgatatag tttatatggg taataaggct ctttatttaa
    30601 tccttatctt ttccttatgg ccagtaggta tagctacggt tattggatta actattggtt
    30661 tattacagac agtgactcaa cttcaagagc agacacttcc ttttggtata aagcttatag
    30721 gtgtctcaat atctttgcta cttctttctg gatggtatgg tgaggtttta ttgtcttttt
    30781 gtcatgaaat aatgttttta attaagagtg gggtttgatg gacatttcaa gctggttcga
    30841 aagtattcat gtgtttttaa tactcctgaa cggcgttttt tttagattgg ctccattatt
    30901 tttctttctt ccatttttaa ataacggtat aatttctcca tctattagaa tacctgtgat
    30961 ttttcttgtt gcatcaggat taattacttc tggtaaggta gacataggtt cttctgtttt
    31021 tgaacatgtt tatttcctta tgttcaagga aataattgtt ggcctccttc tctctttttg
    31081 cttgtctctt cccttttgga tatttcatgc tgttggtagt attattgaca accagcgtgg
    31141 ggcaacgctt agtagttcaa ttgatcctgc caatggtgtt gatacgtctg aattggcaaa
    31201 atttttcaat cttttttctg cagttgtatt tctatacagt ggtggaatgg tctttatttt
    31261 agaatccata caattgtctt ataatatatg cccgttattt tctcaatgtt ctttccgtgt
    31321 ctcaaatatc ttaacatttc tgactttatt ggcaagtcag gctgttattt tagccagtcc
    31381 tgttatgata gtattgttac tatcagaagt attacttggt gtattatcga gatttgctcc
    31441 gcagatgaat gctttttccg tatcattaac tattaaaagt ttacttgcaa tatttattat
    31501 tttcatctgt tcttctacta tttacttttc taaagttcaa tttttcctcg gtgaacataa
    31561 atttttcaca aatctatttg ttagataaaa tattatggca aataaaacag aaaagccgac
    31621 acctaaaaaa ctaaaggatg ccgcaaaaaa aggacagtca tttaaattta aggatttaac
    31681 gacagttgtt attattctgg tagggacatt tactataata tcattctttt ccttaagtga
    31741 tgtaatgctc ttatacagat atgtaataat taatgacttc gaaattaatg agggtaaata
    31801 cttttttgca gtggttattg tcttttttaa gataattggc ttcccacttt ttttctgtgt
    31861 tctttcggct gtgttgccaa cattggttca aacaaagttt gttcttgcga ctaaagctat
    31921 caagattgat ttttcagtat taaaccctgt taaagggtta aaaaaaatat ttagtataaa
    31981 gacaataaaa gaatttttca aaagcattct gcttcttatt attctagcat taacaaccta
    32041 tttcttttgg attaatgacc gaaaaataat tttttctcag gtgttttcta gtgttgatgg
    32101 cttatatctt atttgggggg ggctatttaa ggatataata ttatttttct tagcattttc
    32161 tatttttgtt attattcttg actttgtgat tgagttcatt ttatacatga aagatatgat
    32221 gatggataaa caggagataa aaagagaata tatagagcaa gagggacact ttgagacaaa
    32281 gtcgagaagg cgtgagttgc atatcgagat tctttcagag cagactaaat ctgatatacg
    32341 taattcaaaa ttagtggtaa tgaacccgac tcatattgca attggtattt attttaatcc
    32401 agaaatagcg cctgcacctt ttatttctct cattgaaact aaccagtgtg ccttggctgt
    32461 cagaaaatat gcaaatgaag ttggtatacc aactgtgcgt gatgtgaaat tagctagaaa
    32521 actatataaa acacatacaa aatatagttt tgttgatttt gaacacttgg atgaagtcct
    32581 acgtcttatt gtttggcttg agcaggttga aaacactcat taataagtaa aggagatgtg
    32641 ttatatggga gttaatttct gtaataaaat aggtattgat cagtctgaat ttgaaataga
    32701 atcttccatc attaactcca ttgctaatga ggtattgaac ccaatatctt tccttagcaa
    32761 caaggatata ataaatgttt tactcagaaa gatttcatct gaatgtgacc tggttagaaa
    32821 agacatttat cgttgtgctc ttgagttggt cgttgaaaaa acacctgatg atttataatc
    32881 attcataata actcttttgt tgatataaat atttttgttg aagatattta tgtagtcaca
    32941 ggatcaattg tagaaaattg ttatgttttt gttaggaact gaactaaata atatatgata
    33001 agacagcaaa aacgactgac cataatcctt cttttattag gagttgataa gagagactat
    33061 tcatcctgta atgttaaaac tctgttatat agtattcgcg attatgcaaa gtctgtaaat
    33121 gatcatgaga tactaaccga gagtaatcgt ttattatctc attgcatttc agatagtaac
    33181 ggagctttct ttaaaagtag taaatacgtt ccccttaagt acttacgtaa aaggagaata
    33241 gcacgaaaga taccaaatga ttagcacttt gctagtaagc ttgctggtaa actatattga
    33301 ccgttgggtt tgttttgaga ctgtaattta aattgtgtaa ttgcctgttt ttgatatgtt
    33361 tactccaaca acggagacag gtaaattatg gacgaaaaga agcttaaagc ccttgcggct
    33421 gaactggcta aaggcctcaa aactgaggcc gacctcaatc agttttcccg tatgctgacg
    33481 aaatatcccg gctcttaaaa cgatagtggc tcaggctttc atttttcagc gttccccaga
    33541 agatttccat cggagcgttg tcgtaacagt tacctttacg cgacattgat gttttcagac
    33601 cagactgctc ctgtatgacc cggtaatcgt atgcgcagta ctgtgaacct cgatcggtgt
    33661 ggtggattag cccggcaggt gggcgctggc tcctgagcgc cataaacagg gctttacccg
    33721 tcaattcttt cgtcatgcgc tctcccatgg cgtaaccgac aatttcgcac gtataaacat
    33781 ctttgacgcc ggcgagatac agccatcctt cccgggtggc aacatacgtt atatctgcga
    33841 cccagacctg attaggtgct gtgggcgtaa agttctgatt cagcagattc ggcgtaaccg
    33901 gcggattgtg gtcagag
//
LOCUS       DAJVOY010000026        32946 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVOY010000026 DAJVOY010000000
VERSION     DAJVOY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.026"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..257
                     /locus_tag="OQL90_001086"
     CDS             <1..257
                     /locus_tag="OQL90_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1367940.1"
                     /translation="LDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSVV
                     MTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            267..1641
                     /gene="gspE"
                     /locus_tag="OQL90_001087"
                     /pseudo
     CDS             267..1641
                     /gene="gspE"
                     /locus_tag="OQL90_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1631..2833
                     /gene="hofC"
                     /locus_tag="OQL90_001088"
     CDS             1631..2833
                     /gene="hofC"
                     /locus_tag="OQL90_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCS1367941.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="OQL90_001089"
     CDS             complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="OQL90_001089"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1367942.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(4067..4111)
                     /locus_tag="OQL90_001090"
     CDS             complement(4067..4111)
                     /locus_tag="OQL90_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367943.1"
                     /translation="MIIKTLKMSARKRN"
     gene            4136..4756
                     /gene="coaE"
                     /locus_tag="OQL90_001091"
     CDS             4136..4756
                     /gene="coaE"
                     /locus_tag="OQL90_001091"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1367944.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4756..5499
                     /gene="zapD"
                     /locus_tag="OQL90_001092"
     CDS             4756..5499
                     /gene="zapD"
                     /locus_tag="OQL90_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1367945.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5509..5706
                     /gene="yacG"
                     /locus_tag="OQL90_001093"
     CDS             5509..5706
                     /gene="yacG"
                     /locus_tag="OQL90_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1367946.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="OQL90_001094"
     CDS             complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="OQL90_001094"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1367947.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6262..8967)
                     /gene="secA"
                     /locus_tag="OQL90_001095"
     CDS             complement(6262..8967)
                     /gene="secA"
                     /locus_tag="OQL90_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1367948.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9029..9541)
                     /gene="secM"
                     /locus_tag="OQL90_001096"
     CDS             complement(9029..9541)
                     /gene="secM"
                     /locus_tag="OQL90_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1367949.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="OQL90_001097"
     CDS             complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="OQL90_001097"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1367950.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="OQL90_001098"
     CDS             complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="OQL90_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1367951.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="OQL90_001099"
     CDS             complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="OQL90_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1367952.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="OQL90_001100"
     CDS             complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="OQL90_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1367953.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14093..15013)
                     /gene="ddlB"
                     /locus_tag="OQL90_001101"
     CDS             complement(14093..15013)
                     /gene="ddlB"
                     /locus_tag="OQL90_001101"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1367954.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(15006..16481)
                     /gene="murC"
                     /locus_tag="OQL90_001102"
     CDS             complement(15006..16481)
                     /gene="murC"
                     /locus_tag="OQL90_001102"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1367955.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16535..17602)
                     /gene="murG"
                     /locus_tag="OQL90_001103"
     CDS             complement(16535..17602)
                     /gene="murG"
                     /locus_tag="OQL90_001103"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1367956.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="OQL90_001104"
     CDS             complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="OQL90_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1367957.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18843..20159)
                     /gene="murD"
                     /locus_tag="OQL90_001105"
     CDS             complement(18843..20159)
                     /gene="murD"
                     /locus_tag="OQL90_001105"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1367958.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="OQL90_001106"
     CDS             complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="OQL90_001106"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1367959.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21238..22596)
                     /gene="murF"
                     /locus_tag="OQL90_001107"
     CDS             complement(21238..22596)
                     /gene="murF"
                     /locus_tag="OQL90_001107"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1367960.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22593..24080)
                     /gene="murE"
                     /locus_tag="OQL90_001108"
     CDS             complement(22593..24080)
                     /gene="murE"
                     /locus_tag="OQL90_001108"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1367961.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24067..25833)
                     /gene="ftsI"
                     /locus_tag="OQL90_001109"
     CDS             complement(24067..25833)
                     /gene="ftsI"
                     /locus_tag="OQL90_001109"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1367962.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="OQL90_001110"
     CDS             complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="OQL90_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1367963.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="OQL90_001111"
     CDS             complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="OQL90_001111"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1367964.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="OQL90_001112"
     CDS             complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="OQL90_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1367965.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(28214..29218)
                     /gene="cra"
                     /locus_tag="OQL90_001113"
     CDS             complement(28214..29218)
                     /gene="cra"
                     /locus_tag="OQL90_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1367966.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29300..29386)
                     /locus_tag="OQL90_001114"
     CDS             complement(29300..29386)
                     /locus_tag="OQL90_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1367967.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="OQL90_001115"
     CDS             complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="OQL90_001115"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1367968.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="OQL90_001116"
     CDS             complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="OQL90_001116"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1367969.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPISISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31735..31779
                     /locus_tag="OQL90_001117"
     CDS             31735..31779
                     /locus_tag="OQL90_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1367970.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="OQL90_001118"
     CDS             complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="OQL90_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1367971.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7908 a         9254 c         8017 g         7767 t
ORIGIN      
        1 gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc acccgctatg
       61 tttcagccat gagtgtggca aaaggcgtgg tgtcgctgac cgggcaagaa agtctcaatg
      121 gactaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc ggctgggcgc
      181 gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat gtcttccgct
      241 ttgatgacgc caactaagga gcggcaatga atattccaca gctcaccgcc ctgtgtctgc
      301 gttatcaggg agtcttgctg gatgccagcg aagaggtggt tcatgttgcg gtagtcgatg
      361 caccttcgca tgagttgctg gacgcattgc atttcgctac caccaaacgt attgagatca
      421 cctgctggac gcgccaacaa atggaaggcc acgccagtcg cacacaacag acattgcccg
      481 tagctgttca ggagaagcat cagtcgaaag cagagttgct gactcgaacg ttacaatctg
      541 cgctggaaca acgtgcgtct gatattcata tcgaaccagc ggacaatgcc taccgcatcc
      601 gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat gccggagtcg
      661 cattaaccgc cagattaaaa gtgctgggaa acctggatat tgcggaacat cgcctgccgc
      721 aggacgggca attcactgtc gaactggcag gaaacgccgt ctcatttcgt attgcgacct
      781 taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg aaccaggcac
      841 tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct catgccttgc
      901 aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa acggtcacgc
      961 tttatagtgc cctgcaaaag ctgaataccg ctgacattaa tatttgtagc gtcgaagatc
     1021 cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt gccgggctca
     1081 cctttcaggg cgttttgcgt gcgttattgc gccaggatcc tgacgtcatc atgatccgat
     1141 ggcgaaacgg cagaaattgc cattaaagcc gcgcaaaccg gtcacctggt gttgtctacc
     1201 ctacacacta attccacctg cgaaacgctg gtacgtttac agcaaatggg agtcgcccgc
     1261 tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg taaattttgc
     1321 ccacattgtc gccagcagca aggggagccc atccatattc cagacaatgt atggccgtcg
     1381 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt
     1441 accgccttgt ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat
     1501 accgacgttg aatcgctgga aacgcacgca cgacaggcgg gtatgcgtac gctttttgaa
     1561 aacggctgcc tagccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
     1621 gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
     1681 tgcgcaagat gggatgctat gggcagagag ccgtgctttg ctgctcatgg cactacagca
     1741 acagatggtt accccactta gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
     1801 agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct
     1861 ttctgaaggg ctggcactgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct
     1921 gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg
     1981 gtcagaggta tttccgccac tctatcaggc gatgatccgc acgggtgaac tgaccggtaa
     2041 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc agttgaccga
     2101 caaagtgaaa tcagcgttac gttatcccat catcatttta gcgatggcaa tcatggtggt
     2161 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac
     2221 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatggag
     2281 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
     2341 accgacctgg cttatagtgc ggcagaaatt gctgttacgc atcccgatta tgggttcact
     2401 gatgcgggga caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg
     2461 cattactttt ttgcagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt
     2521 gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct
     2581 aaaaaatacc ggggagttta gcccgctctg tttgcaatta gtgagaacag gagaggcatc
     2641 cggctcgctg gacctcatgt tagacaacct cgcccatcat catcgggata acacaatggc
     2701 gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat
     2761 tatcggtacg ctggtggtgg cgatgtatct gccaattttc catttaggcg atgcgatgag
     2821 tgggatggga taacgctggc gtgttgctcc acaccagcgt tggaagatta caggttgttg
     2881 aagatgcggt tttcttgttc ctgcacacgg ataaatgtgg tgcgcttggt cagttctttc
     2941 aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg
     3001 gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcgatat
     3061 tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc
     3121 ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc
     3181 atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg cttttgcgac atcgcccggc
     3241 gtggtgcagc caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat
     3301 tcgattaccg cagaaagctg cggataaccg acgcctgttt tgacgcgagt tgtacaaaca
     3361 gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac
     3421 atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct
     3481 ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacaaag
     3541 tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgta
     3601 ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc
     3661 cactcttcga cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga agccagcgca
     3721 gaggccatag aaaatgtacc tacggtgtcc atatttgcgg cgataatcgg cacgccggac
     3781 cagctctgat ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt
     3841 ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct
     3901 tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag
     3961 tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg
     4021 ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcgctttc
     4081 gtgcactcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatgag
     4141 gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg ccaatgcgtt
     4201 tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc
     4261 aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga ttgctgctga
     4321 tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa
     4381 ctggcttaac gccctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca
     4441 agctacttcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa
     4501 aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat
     4561 gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc aggcaacgcg
     4621 cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat
     4681 cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc
     4741 acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg
     4801 cgtacatggc tgcgcattga gtttttgatt cagcaactca ccgttaattt acccatcgtt
     4861 gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag
     4921 cgcggcgaag tccgcactga gctgttgaaa gaacttgacc ggcagcaacg taaactccag
     4981 acttggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta
     5041 aaagcggcgg ggagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat
     5101 cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat
     5161 ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa
     5221 acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt
     5281 cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt
     5341 ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt
     5401 tccggacata agagccgttt tgccattcgt tttatgccgc tggacagtga aaacggacag
     5461 gtaccggaac gtctagattt cgaactggcc tgttgctaag gagtgaaaat gtcagaaact
     5521 attacggtga attgcccaac ctgcgggaaa acggtggtat ggggtgaaat cagcccgttt
     5581 cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa
     5641 aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag
     5701 cagtgacatt ggtctgatgc cgcacatagg cctgataaga cgcggacagc gtcgcatcag
     5761 gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca gactacagac
     5821 gcttaagctt cgcaattacc ggttcattgg ctggcggaaa atcatcggca ttaagaccga
     5881 ccagcgacat ccactcaccg ggttgccctt ctttacccca cggcacccct tcccaacttt
     5941 cgaccagcca gaaccacaga gttatatgcc tgtccgggaa ttcatatgcc agtttttcaa
     6001 atagcgaaaa atgttgtggg gtaatcccga cctcttcctg aagttcacgc accaccgcct
     6061 gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta ttcgccatgt
     6121 gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt ctcgttgcga ataataccta
     6181 ccgcaatttg cagctttttc attgttttaa acctataaaa aaggcgcaga atcctgcgcc
     6241 ttttgcttca acagttagct tttattgcag gcggccatga cactgcttgt attttttacc
     6301 agaaccgcac gggcaaggat cgttacgtcc tactttgcgc tcaccggttt gcgccgccag
     6361 tgcagctgcg gctgcagagt cgtcatcctg atggctaagc tgctgcattt gcgctaaacg
     6421 ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca tacgtacctg
     6481 aactttgctc agcgtactga taacttcata tttcaacgac tccagcatcg ctgcaaacat
     6541 ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc cacgcaggtg
     6601 gataccctga cgcagatagt ccatcgctgc caggtgctct ttccacaggg agtcaagcgt
     6661 ttgcagcatg acgcctttct cgaagtgacg catcatctca gcaccaacca cttcttcttt
     6721 acgctgatac acttcgatgg actgcgccag aatgcgctca cgcagcgtct cttcatgcag
     6781 ttctggttct ttatccagcc actcggcaat tggtaaatcg aggtcgaaat cgttcttcag
     6841 acgttcctgc agccccggaa tatcccacat ttcttccagc gactgtggcg gaatgtaggc
     6901 atcaatggtg gctttgaaca catcttcacg aatgctgttg atggtttcgc tcacatcgct
     6961 gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt tagccacgtc
     7021 atcatattcc agcagttgct tacgaatgtc gaagttacga ctttcaactt tacgctgggc
     7081 gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct tcatacccag
     7141 tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg catcttccat
     7201 cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac cgcgcaactg
     7261 gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc cacctgcttc
     7321 cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt gctctgcggt
     7381 cggattttcc agcgcggcaa cttctgcctg ccagctacca ccgagcacaa tatctgtacc
     7441 acgacccgcc atattggtcg cgatagtcac cgcagccgga taacctgctt gagcaacaat
     7501 cgccgcttcg ttggcgtgga atttggcgtt caggacgttg tgcttaatac cggctttggt
     7561 cagttcgttt gacaccagct ccgatttttc gatggagata gtacccacca gcaccggctg
     7621 gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt ccgcttcagt
     7681 catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg gaacaacgac
     7741 ggtatccagc ttgtagatgg agctaaattc gaaagcttcg gtatcagcag taccggtcat
     7801 ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgaag ccagcgtttg
     7861 gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca gaccatcgga
     7921 ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca acgatgataa cttcaccatc
     7981 tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg ccgccgttac
     8041 gtggtgcatc agcatgatgt tggccggaga gtacagagac tccccttcat ccatgatgcc
     8101 ctctttcacc agcagttctt caatcagcac cagaccacgt tcggtcaggt tcacctggcg
     8161 agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt cttctttttc
     8221 ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg agctgtcttc
     8281 tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg agtccacttc
     8341 gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga acgccatatt
     8401 gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt cagctgcgta
     8461 agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg ataccgacag tcaggccaag
     8521 gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt cgttgacggt
     8581 aactacgtgc acgcctttac cggttagtgc gttcaggtaa gcaggcagcg ttgcggtcag
     8641 ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa ccataccgcc
     8701 gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct cacgtaccac
     8761 ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac gtgcacgaaa
     8821 ctctgcggtt ttccctttca gttcttcgtc ggagagtttt tccatctccg gttccatggc
     8881 attgatgatg ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac gactaccgaa
     8941 aactttagtt aacaatttga ttagcataat aaaatctcaa acgccccgcg ttgcggagtt
     9001 aataaaatga agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca gcacggatgc
     9061 cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgtggggta aaatgcgcat
     9121 aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcagcagc gcgctgagcg
     9181 tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca acgggcagtg
     9241 tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt gcgaatggca tattgatgcc
     9301 agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt cgcttccagc aaggccaatt
     9361 gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt gcgggcacgt
     9421 ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcgacc atccctaata
     9481 agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt attccactca
     9541 cgttattgcc atcccgatcg aattcaaacg tatttagcgc acaaatctta tcattaaagc
     9601 ctgaagtcca cagcatgaat gaagataagt tgtgcgtaaa agtgcttaaa aaaacagcgt
     9661 tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa acgactggtt cacctggccg
     9721 gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc gttatgccag
     9781 tacagctgaa ggcgctttga aggccaacgg cagttctgcg tcgtcctgga aggtcacata
     9841 ttcccaagct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca gtgcatgacc
     9901 ggatttataa gcgataaatg caccaataat attgtgacca cacatgaaca agtcaccgat
     9961 cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt cttcgttcag
    10021 tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc acaaaccacg
    10081 ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc tgatctggcg
    10141 cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat caatagccgg
    10201 atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa attcagccca
    10261 cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt tggcgcagtt
    10321 caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc tgccgtccat
    10381 gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca ggcccgcgag
    10441 agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga ccagacacgt
    10501 acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg gtggattcaa
    10561 gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg tcagagtgac
    10621 tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa gtgtcctttg
    10681 tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact atacattcgg
    10741 cgggccagtt tagcacaaag agcctcgaaa ccaaaattcc agtcaattct taatcagctt
    10801 gcttacgcag gaatgctggg atatccagat aatccggctc tttcgcagtt tgcggcgcat
    10861 tgtcattcac gactttagca accggcttct gctcctgggt cagcggagcc atcccatgct
    10921 gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga gtgatttcag
    10981 gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg tcattcatat
    11041 ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat gcacggatgg
    11101 tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg atgttaacca
    11161 gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag atagccattt
    11221 cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc attgcgtagc
    11281 ccatctcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa cccggacgag
    11341 taatcagttc agcgatacct tgcacagcgc ctttcagtac atcgttcgct gcgccaaacg
    11401 catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc gggatagtga
    11461 tcagagagtc cacatgcttg gacagttcag tgatcccctg ctccgcgaat gccatacgct
    11521 tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata cccaaatctt
    11581 ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc atacccgcag
    11641 caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga tcctcatcag
    11701 ccgcattgcg gccaacttct ggattagcgc cagcgcccag tcctttggtg ataccgctac
    11761 cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg gtatttaccg
    11821 cgaagaattc aacaccttca atgcgctcgc gcaccatgtg ttcaacagca ttaccgccgc
    11881 cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt ggttcaaaca
    11941 tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa tcatcgtcgg cctcataaaa
    12001 attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca actgatgctg
    12061 taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca tagtgaagca
    12121 atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg gtaatgttca
    12181 gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct gcaagacctt
    12241 cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt ttaacccctt
    12301 gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg agcagctcgg
    12361 tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgt ggcggacgac
    12421 cgcctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc agcgcacaac
    12481 cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg taagcgatat
    12541 cactggtcac gacattgcca gcataaggaa ttaccttagt gtggcgcaat gccccaccgg
    12601 tataaacggc gatatccatt gtaccacctc cgatatcgac gacgcagaca cccagttcac
    12661 gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc agttggtcaa
    12721 ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg ttgtgacatg
    12781 tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag tcctaccgga ttcttgatcc
    12841 cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga tgttcatcgc
    12901 gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc gtcacttctt
    12961 cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta ccagaaagcg
    13021 ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca atggcgcgtt
    13081 gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata ccacgtgacg
    13141 ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc cctactaaag
    13201 cggcaacctt cgcggtacca atctccagtc ctactaccag ttttctgtcc gtcgccttga
    13261 tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg gcggcaaggg
    13321 cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa tccgtttgcc
    13381 atcggtttgc gccagctgct gtaaaaccgg ataaagttct acaaagcgag ccaaacgttt
    13441 catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca actgccagga
    13501 acgccgcgcg gtcatcgccg tttccttcag agtaaatctg tcctttgcca gcatctgccc
    13561 catttcgcga tagccctgca acacttcatt ggcgctgcct tccgggccat acagcattgg
    13621 aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt ccgcgtctac
    13681 catatgttga tcattccacc gcgcaatcgg cacatattca accagatgaa tcttcaattc
    13741 atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt gttctatttg
    13801 cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca atgccaggat
    13861 cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca ccagctttga
    13921 gagcggcagg cgttgcgcat cttccatcca gcccaacacg acccagccgc tcaccaacac
    13981 tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat tattgcggcg
    14041 agaagaaacc tcttcttcgc tgtttcgcgt gttcagagca gcctgcgaca tattagtccg
    14101 ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga cgtgccgcca
    14161 tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc agataaaact
    14221 gtccatcgct gtccagcata acgtcaatac gtccccatcc tttgcaacct aacgtcgtcc
    14281 atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcatccaga cctgcggggc
    14341 agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag gttccggacg
    14401 gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg aactccggcc
    14461 cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat cttaatgcat
    14521 cttgtagagc attttctgct actacttttg acattcccac actggaacct tcgcggctcg
    14581 gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta tcgctcaggc
    14641 ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa ccggcacctt
    14701 gccacagcag tttgctgcgt agtttatcca ttgaaagcgc agatgccatc actccgctgc
    14761 cggtataagg caagcccatc agctcgagca tgccctgcag cgtaccatct tcaccgccgc
    14821 gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc gtcacgtcga
    14881 cttctttcgg gtcgacagga tacgcatcaa taccaccttc acgcagcccg gctaacaccg
    14941 ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac aggaccgcga
    15001 ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat ttcagctaaa
    15061 gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc gttaccggtt
    15121 aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat gggatcaatt
    15181 ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg aattggcgct
    15241 tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag cacattggcg
    15301 aaatcatcat acaagtcgcg cgtacgggta aaacggtgcg gctgaaacag cattaccagg
    15361 tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc cgtcgggtgg
    15421 tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg ctccagcggg
    15481 aattcaccga ggaaatcaaa acggcgacca gtccccagga agctctccag cgcacgcaaa
    15541 atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc ggcgttcagc
    15601 gcgttatgac ggcctggcgc attcagggtg acgcgcatcg gctctttgtc ctggcgcagc
    15661 agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac gtcggcatct
    15721 tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg gtaacaattc gcggatcacc
    15781 ggatcatcaa cacacatcac cgcacgaccg taaaacggca ggttgtgcag aaaattaata
    15841 aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc ggcttcgata
    15901 ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact ctcatctgct
    15961 tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc ttttaccagc
    16021 ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga aaccatcgcg
    16081 gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa acgcattaac
    16141 tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc tgcgacaatt
    16201 tccgggttat cggcagaaat ggcgctggaa acaacgacca cgctggcatc acgtacgttt
    16261 tccgggcgat ggttgaaata aatcgtcgct cccagattca ttaactgctg cgtgaccgga
    16321 tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac ttcggcaata
    16381 ccgcccatac cggcaccacc gatgccgaca aaatgtatgt gccgaacgcg acgcatttcg
    16441 ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc cattaacttc
    16501 ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc cggctcactt
    16561 catttgccac tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt tctgccatgg
    16621 ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc acgctaagct
    16681 gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca ttccagtatt
    16741 gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc gcggcgattt
    16801 cactcaccgt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca tacgccgccg
    16861 ccatatcatc aataaattcc gtcactttat gctgcggttg ccccgcttcg gcatacgcct
    16921 gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca gatagtgact gaatcaccca
    16981 gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg gaaccaccca
    17041 ctaccagcac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc aacggcagcg
    17101 ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa gcgcctggaa
    17161 acgcctgcat cactttggtg gcaatcttcg ccagccattt attggttaag cccgcaatac
    17221 cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga ccacctggac
    17281 ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc ataatcgccc
    17341 gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct tttatacctt
    17401 ttccacgcag accagagata cgaatgaaat caatttcgat gccatgtttt ggcactaagt
    17461 ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga gccattagat
    17521 ggtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc atcaccatta
    17581 atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt tctccagacg
    17641 cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca gtaagctcga
    17701 accaccgtaa ctgatcagcg gcaatgtcag acctttggtc ggtaacatcc ccgccgccgc
    17761 gcctacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg cgagaaaacc
    17821 ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc gacatcgcgc gaaaagcgac
    17881 gaagaatacc attaaaagtg ccagcaccac accgacatac cccagttctt cgccgataat
    17941 ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta ccgagttacc
    18001 taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg ttaactgata
    18061 gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc ggatacggta
    18121 cggttcggcg agtatcagca acacaaccgc tgaaatgccc ataccgataa tggcaatgaa
    18181 ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa acaccaccac
    18241 caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa tcacgcccat
    18301 cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca ccagatagtt
    18361 ggcgatataa caaaacagcg acagttttgt cagctccgca ggctggatac gcagcaaacc
    18421 gagatcgatc caacgcgatg cccctttaac cgagctcccc actaccagca cgatcatcag
    18481 caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact ccatcggcag
    18541 acgcagcgta atgatcgcca gaatgaatgc caggatcaga tagacgccat cacgcttcgc
    18601 gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg aggtcaccat
    18661 gataaagcca atcgccgcaa ggccgaaggt cagccacagt aaggtgcgat cgtacatgat
    18721 caggctgtcg gtatcttttt cccgcgagcc catcacccag ccttttagcg ccgtggagat
    18781 ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga gagataaacg
    18841 catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc aaagttcttg
    18901 aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccgg ctgaacacgc
    18961 ggagccagca agcgcatcgc ctgttccata gtttcggttt gttctgccac ttccgggcgt
    19021 agcgccgcca gctgcgcacc gtcacgacca aaacagtaca aacgcacgtt gtcgccattc
    19081 agatagcgcg ccagtggact gaagtccgcc gatttaccat cgccgcccag caacaaatgc
    19141 agcgtgccgt ctacctgcaa gccattcaac gccgcttccg tactgccgac gttagtggct
    19201 ttcgaatcgt taatccagcg tacgccattg tgctccagca ccacttcaaa gcgatgcggc
    19261 agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggcaaacc agctgcgtct
    19321 gccagtgcca gtgctgccag cgcattggtg tagttatgct gcccggaaag tttcatctct
    19381 ttcacgttca gcactttctc gcctttcact cgcagccagg tttctccctg ctgatgattc
    19441 aggtgatagt cacccatgtt gacgccaaag ctgacgcagc gttcatcagc accgcgaatc
    19501 ggcattgtta aggcatcatc agcattgacg acacaaactt tcgcattttc gtaaatacgc
    19561 aattttgccg cacggtattg ctgcaaacca aacggatagc ggtccatatg atcttcagtc
    19621 acgttcagaa tggtcgccgc caccgcctgt aagctggaag tggtttccag ctggaagctc
    19681 gacagttcca gcacgtacag ttcgcactca gcatcaagca gcatcaacgc tggcaggcca
    19741 atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc cactagcgtg
    19801 gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg tgcttcgcgg
    19861 cagaacagct cgatatcgcc aacgatttcg attccggcat cagcggcagc gcttaaggat
    19921 ggatgcgcca gtgcaatacc gggactggcg acaatcagat ctgccgccat cagccattca
    19981 tcattcagac tgcccgtgtg gcgttctacg gcttcgggta atttatccag gccaggcggt
    20041 gtcatacgcg tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa gtccacgcag
    20101 gaaagcccgg tgaggcccag gccgataatg acgacatttt taccctgata atcagccatg
    20161 attaacgtac cttcagcgtt gccagaccaa tcagaaccag catcagcgaa ataatccaga
    20221 aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga tgaatcggtg
    20281 ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg atgacagaaa
    20341 gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga cgtagcagta
    20401 cggcgataat gcctaacgca ccacctaacg ccagcgaacc tacatcgccc ataaagacct
    20461 gcgccggata ggtgttaaac cacaggaagc ccagtcctgc cccgactatc gcggtacaga
    20521 caataaccag ttccccggcg tgtcgcagat acggtatgtg taaatagctg gcaaagttca
    20581 tgttgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg gtcggcataa
    20641 ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc acaatgacga
    20701 agtaagccag cagaatgtag aacagcccca actgcggcat cacatcttta aagaatggga
    20761 ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta cagggcgaag gcgacaccca
    20821 gcgcaatgac cgacatccag aaatacttcc aacgagcgat caaccctttg gtgtctttac
    20881 gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc agcaccacca
    20941 acacgcacca gacgtacgga ttggacgggt aagcccacag cagtacggag atcacaatcg
    21001 ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg cttgctgaag tgtgattcag
    21061 gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg caaatgagca atcatacgcg
    21121 ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg cgaaacgtca
    21181 gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc agccaaacta
    21241 acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcggcac tacgtgaacc
    21301 cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac gcgtaattaa
    21361 cgcagtttta tcagcaaaat gttcgccaac gccgctggcg gtgctgatag catggctttg
    21421 tttacccacg cttaacacgc ggtcaatacc agcagctttt gccgcctcgc ccacctgtac
    21481 atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca gcacgcggta
    21541 gcccggcatt tcagccagta cctgaacagc ggcggtcatt gaaccgacat tggcgttgta
    21601 ggagtcgtcg agcagcaact ggttttctgc cagttggatg gggaacagac ggcctggaac
    21661 agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca cggacatgga
    21721 gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg gcagcagaac
    21781 atcgacgcta ccggttgggg tttgtagggt aaattccgta ccgtgcgagg tcacatggat
    21841 attggtggcg gtgaaatcgc tgttggcggc attgggtgag aaacgccaca ctttgcgtga
    21901 gccaattacg ctctgccagt tcagccagtc gttgttgtcg gcgttcataa tggcgatacc
    21961 gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg caagcgagcc
    22021 aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gcagcttccg ggcgagtcag
    22081 actcacagtc caggctattt cgccctgatg gttcgcgcca agttcaataa ctgcgtaatc
    22141 gtattccggc gttaagcgca acaacgtcat cggtacaccg atgtcgttgt tgagattgcc
    22201 tgccgtataa agcgtgttgc cgcactggct taaaatcgcc gccgtcatct ctttaacgga
    22261 ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc ggaacttgct ggcgaaccca
    22321 tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg gcaggtcgat
    22381 atccagcgga cggctgacca gtagtgcgcc cgcgccgcca gctttcgcct ggtcggcaaa
    22441 atcgtgggca tcaaaacgtt cgcctttcag ggcaacaaac aggcagcccg gcgtcagttt
    22501 tcgggtatca gtggttacag catcaagggt gatatctgca ccttgcagtt caccgttgag
    22561 aatgtcggta agttggctaa gggttacgct aatcatgcaa tcacccccag cagacgcgcc
    22621 accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta atcttcatgg
    22681 cctttgcccg cgaccagcac cacatcgttc tctttagcct gcataacggc gcaagtcacc
    22741 gcttcagcac ggccttccat cactttggca tgtccggcat ctaacattcc cgccagaata
    22801 tcgttgatga tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac caccgccacg
    22861 tcagcaaact cttcggcaat tgcgcccatc agtggacgct tacctttatc gcgatcgcca
    22921 ccacagccaa agacacacca cagcttgccc gcacagtgca gacgcgccgc ctgtaaggct
    22981 ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt gcctggcaca
    23041 gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt caacagatca
    23101 gccaatggat agccgagtgc caacagtgtc gccagcgcga gcagcaggtt gctgacgtta
    23161 aaagcgccta tcagatggct ttcaatttcg ccatcgcccc aacttgagct aaagcgaatc
    23221 gtcacaccgc tgtcgtgata gttcacgtcg atcactttta accagcgtcc gtgacagttc
    23281 ggattaatat gatcttccat tgataccgca accgcgtccg gcagctttgc cagccaacgg
    23341 cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgttc agaataaagc
    23401 agccatttcg cagcttcgta gtgttccata tcaccatgat aatcaaggtg atcgcggctt
    23461 aagttggtaa agaccgacgc cgcaaatttc aatgccgcca cacggtgctg taccagcccg
    23521 tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct ccaccagccc cgccagctca
    23581 tgctgaacat cgactgccga accggtggta ttttctgtcg ggatcacttt ccccagcaga
    23641 ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct ccactgcgcc
    23701 agcagctggg tagtcgtggt tttgccgttg gtgcccgtta cgcctacgag acgtaaattg
    23761 tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcgtt gagctggctg
    23821 agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc ctcatctttc
    23881 gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata tcgacgcccg
    23941 tccgcctgat gacctactac agctacaaag agatcgcccg ccgcagccac acggctgtcg
    24001 agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc aagaaggtcg
    24061 cgcaaattac gatctgccac ctgtcccctc gccttgatta atcacaaatt catttttatc
    24121 gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc ccatgatggc
    24181 accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg gatcgttgat
    24241 aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg tataagcaat
    24301 atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg ttttaatggc
    24361 gatacgatag cctttaatcg ccgccttcac gccgccgccg cccggtagcg ccacgctttc
    24421 catcatatgc accacggtgc ggacaatgga ttccgggaag acacgttcac cgggaaccgg
    24481 ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga tagttgcgta
    24541 gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga aggtggccct
    24601 ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc cgaccaaccc
    24661 caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg ctgaggacgg
    24721 catcgctaac gccagcttgg aaacaccgac gttactcgac ttctgtaata ccccggtcag
    24781 ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc gataaggaat
    24841 ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca tcaccaccat
    24901 cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac gcatcgcctc
    24961 tttcggcgtg ccgctcagat tgttagggtt gtatgacggg ctgttagcca tcgccagcac
    25021 ttcacctgtg ttgacatcca ccagcacggc gctaccagat tcagccttgt taaaggccac
    25081 cgcgttgttc agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac tcagcgccag
    25141 gttgtgcgct gcctggctgt cagtagaaga aatatcttca attacgcgac catagcggtc
    25201 tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac tcttctcaac
    25261 gccctcaatc ccttgactat cgacgttagt aaagccaatg aggtgagcag tcacttcgcc
    25321 ggatggataa taacggcgag attcttcacg cagatgaata cccggcagtt tcagtttttt
    25381 gatgtagtcc gccatgtcag ggtttacctg acgcgccaga taaataaagc gccctttcgg
    25441 gttggcgtta atgcgggcag aaagctgatc cagcggaata ttgagcgcgt tagccagcgc
    25501 cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg ggtcagccca
    25561 aatcgctttt accggcacgc tcaccgctaa cgggcgacca gaacggtcag taatcatgcc
    25621 gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt tcaccagcat
    25681 atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca gcgccaggag
    25741 aatacagccg cataacaacg caaaacgcca actaataaag ttggcatgtt cttcctgacg
    25801 ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt atttttgcac
    25861 tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg tggcgatcct
    25921 ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc gccattcaat
    25981 gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta aacgggtatg
    26041 gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg caaatgaaca ggcagagtgg
    26101 cagcttccca aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat ggcgctcgtg
    26161 gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga tcatgcgttc
    26221 gtcctctctg cgatacgcag aactgaacta cgggcacgag gattctcagc cacctcttct
    26281 tcgcccggca ttaacttgcc taatgctcgc agctgacggc cacccagttt tttgagctgc
    26341 tcttcagtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc acgcataaaa
    26401 cgtttcacaa tacggtcttc cagcgagtgg aagctgatga tcgaaagccg cccacccggg
    26461 gccagcacgt tgagcgagct ttttagcgcc tgctctatct cctccagttc actgtttacc
    26521 caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc tttcaccggc
    26581 gttgcagcag ccacgacttc cgccagttct ttggtgcggg tcatcggctg ttcgcggtta
    26641 cgctcgacaa tggcgcgggc aatgcgtttg gcaaaacgct cttcaccata ggttttcaat
    26701 acccaggcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga ctgcccacgg
    26761 gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc acgttcagca
    26821 tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat cttgccgata
    26881 agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat gatggagaag
    26941 cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc gatcgccagc
    27001 aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtgaccacc gcgaccaaaa
    27061 gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc ttcatccagc
    27121 agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct gcagtcgctc
    27181 cgataagtct ccagtagcca actgctctgc gtcgatatct tccttgacct gttgatgcca
    27241 ggttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca cttcttttgt
    27301 cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc catccatctg
    27361 acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaactg ggttcatgct
    27421 cgacagacgc gataattttt gctcgataat ttcccattca ggcagggggt aaagcagcag
    27481 gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct caagcagctg
    27541 ttcccgataa cgggtaggca ctgataagcg ccctttgctg tcgagattga ctaacgttgc
    27601 tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt tcccactgaa
    27661 aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata ccgaggtaaa
    27721 aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg cgcgaagcaa
    27781 attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcactgt tttaaaatga
    27841 ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg cattttagga
    27901 catatcttcc ccggttaaca gtccttgtta cgtctgtgtg gcgcttgatg ccagcgggtt
    27961 tgcgcaaaag caaagaaaag tgtttgttaa atcggcaatt catcgccgca tgtaacaaaa
    28021 taatgcaaaa acgcgctgtc gatatcactt aattattgtg aatttcagct ggtgagttaa
    28081 ttaacgggcg ggtttaaaac catttatcaa taatagtcag gaatagtctt atttacttta
    28141 agcatattga tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat
    28201 ttttgttcgc ggcttagcta cggctgagca caccgcggcg atagagatta cgtttaatgc
    28261 gcgttaaacc aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca
    28321 cacgctctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac tgtaagaagt
    28381 cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttaccatcgc
    28441 gacgtagcgt gacatccatc accccttgca acaacgcaaa cgacgttgtg aacagcgcct
    28501 gcggcatcag atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat
    28561 agctgttggc atacaggaaa tgcacttcgc gcggatcatc tttccaggca gtacggaaac
    28621 cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct
    28681 cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa
    28741 ccacgctggt gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggg
    28801 cgttagccca gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg
    28861 catcaacctg acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt
    28921 ctgagcaggc aatcagcagt tgataacccc gttgccgcgc ctggcgctca agatagttag
    28981 cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc
    29041 gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag
    29101 ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa
    29161 catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa
    29221 ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt
    29281 aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt
    29341 tcgcaaaaaa agcccagcgt gacatgttgg gctgcaaatt gcacactgag atcattctca
    29401 acgcattatt ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat
    29461 tttcgccacc tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa
    29521 ttgaacggta taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt
    29581 acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg
    29641 ctcaacatgc gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa
    29701 ttgcttttcg atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga
    29761 taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga
    29821 aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat
    29881 ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg
    29941 cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg
    30001 cgattattgc gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga
    30061 gagatctgga tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat
    30121 tgcatataag attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc
    30181 cccagatagc ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta
    30241 gacagttcct gaatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct
    30301 tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgagg
    30361 ccaccggaat tgatccagcg acgcggtttg tcgaatggat aataaagtgc agcaaacatc
    30421 tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg ccaaagagtc
    30481 tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgacga
    30541 gcgcgccact gttcaatttg ctgccaccag tcgcggatct catccagtgg ttgatgggcg
    30601 gattcttgcg acaagagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc
    30661 gggatatccg cagtcacggt tttagaaatg gaaataggat caatatcgat atgcagaaca
    30721 gtggcatttg ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg
    30781 acggcgaaaa tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt
    30841 cccagcatgc ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag
    30901 gaaacaacgg gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc
    30961 atgattgccc caccgcctac gtagacaacc ggtttttttg ccactaccag cgtttgcaga
    31021 gcacgcttaa tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc
    31081 gactccggcc agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca
    31141 acgactactg gtccaggacg accacttgcc gccagccaga aagccttttt cagcacctgc
    31201 ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa ccactggtcg cgaaatcccc
    31261 accatgtcgc actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa
    31321 aggacaacta atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc
    31381 gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg cgccagacca
    31441 tcggccatat gcactgccgc ctgctcatga cgaactaaaa catgatcgat accacccacg
    31501 gtatgcaatg catcgtaaat atcaaggact gcgcctccag gataaccgaa tacttgttta
    31561 acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc
    31621 tgcctcactg tttgatggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag
    31681 caattggcag ccagagacat aaacgcaggg taataagaat aaacaggagg aaagatgaaa
    31741 agaaacgata aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca
    31801 caacttacag caatttctct atttttttaa ttaatcctaa aatagagagc ccacacattc
    31861 agaataaaat tctacattca ataaaaaatg cagaataaac cagacattca tgtctgacct
    31921 attctgcatt cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc
    31981 ctttatcgcg cccggcagct tcatgccatg agagataaca ggttctgctg ttttgtttta
    32041 acggcagcgg taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg
    32101 gcgcaatagc gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc
    32161 cctgatacgc gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg
    32221 cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg
    32281 ggcctttaat tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg
    32341 gcacgctggt aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct
    32401 gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat
    32461 ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc
    32521 tgcaaacaca aagatgaaat acacgttcac tactcgcggg ttcaaaacct gaaccaggca
    32581 attcattttg tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag
    32641 cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca
    32701 ggcgtgcaac agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg
    32761 taatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc
    32821 gtagctgtgg cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac
    32881 ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg
    32941 aagaat
//
LOCUS       DAJVOY010000027        32379 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVOY010000027 DAJVOY010000000
VERSION     DAJVOY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.027"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            197..991
                     /gene="panB"
                     /locus_tag="OQL90_001119"
     CDS             197..991
                     /gene="panB"
                     /locus_tag="OQL90_001119"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1367972.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1003..1854
                     /gene="panC"
                     /locus_tag="OQL90_001120"
     CDS             1003..1854
                     /gene="panC"
                     /locus_tag="OQL90_001120"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1367973.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OQL90_001121"
     CDS             complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OQL90_001121"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1367974.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3316..3696
                     /gene="panD"
                     /locus_tag="OQL90_001122"
     CDS             3316..3696
                     /gene="panD"
                     /locus_tag="OQL90_001122"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1367975.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3700..4900)
                     /locus_tag="OQL90_001123"
                     /pseudo
     CDS             complement(3700..4900)
                     /locus_tag="OQL90_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(4995..5435)
                     /locus_tag="OQL90_001124"
     CDS             complement(4995..5435)
                     /locus_tag="OQL90_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1367976.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5540..6310)
                     /locus_tag="OQL90_001125"
     CDS             complement(5540..6310)
                     /locus_tag="OQL90_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1367977.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6307..7233)
                     /locus_tag="OQL90_001126"
     CDS             complement(6307..7233)
                     /locus_tag="OQL90_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1367978.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7342..8004
                     /gene="can"
                     /locus_tag="OQL90_001127"
     CDS             7342..8004
                     /gene="can"
                     /locus_tag="OQL90_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1367979.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="OQL90_001128"
     CDS             complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="OQL90_001128"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1367980.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8787..11177
                     /gene="gcd"
                     /locus_tag="OQL90_001129"
     CDS             8787..11177
                     /gene="gcd"
                     /locus_tag="OQL90_001129"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1367981.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="OQL90_001130"
     CDS             complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="OQL90_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1367982.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12940..13287
                     /locus_tag="OQL90_001131"
     CDS             12940..13287
                     /locus_tag="OQL90_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1367983.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13393..14259
                     /gene="speE"
                     /locus_tag="OQL90_001132"
     CDS             13393..14259
                     /gene="speE"
                     /locus_tag="OQL90_001132"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1367984.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDRQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14275..15069
                     /gene="speD"
                     /locus_tag="OQL90_001133"
     CDS             14275..15069
                     /gene="speD"
                     /locus_tag="OQL90_001133"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1367985.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15106..15468)
                     /locus_tag="OQL90_001134"
     CDS             complement(15106..15468)
                     /locus_tag="OQL90_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1367986.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15643..18240)
                     /gene="acnB"
                     /locus_tag="OQL90_001135"
     CDS             complement(15643..18240)
                     /gene="acnB"
                     /locus_tag="OQL90_001135"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1367987.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGLLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFMNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18595..20448
                     /locus_tag="OQL90_001136"
     CDS             18595..20448
                     /locus_tag="OQL90_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCS1367988.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(20519..21943)
                     /gene="lpdA"
                     /locus_tag="OQL90_001137"
     CDS             complement(20519..21943)
                     /gene="lpdA"
                     /locus_tag="OQL90_001137"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1367989.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22268..24160)
                     /gene="aceF"
                     /locus_tag="OQL90_001138"
     CDS             complement(22268..24160)
                     /gene="aceF"
                     /locus_tag="OQL90_001138"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1367990.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24175..26838)
                     /gene="aceE"
                     /locus_tag="OQL90_001139"
     CDS             complement(24175..26838)
                     /gene="aceE"
                     /locus_tag="OQL90_001139"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1367991.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26999..27763)
                     /gene="pdhR"
                     /locus_tag="OQL90_001140"
     CDS             complement(26999..27763)
                     /gene="pdhR"
                     /locus_tag="OQL90_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1367992.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28304..29677
                     /gene="aroP"
                     /locus_tag="OQL90_001141"
     CDS             28304..29677
                     /gene="aroP"
                     /locus_tag="OQL90_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1367993.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29720..30574)
                     /gene="ampE"
                     /locus_tag="OQL90_001142"
     CDS             complement(29720..30574)
                     /gene="ampE"
                     /locus_tag="OQL90_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1367994.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30571..31122)
                     /gene="ampD"
                     /locus_tag="OQL90_001143"
     CDS             complement(30571..31122)
                     /gene="ampD"
                     /locus_tag="OQL90_001143"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1367995.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31210..32103
                     /gene="nadC"
                     /locus_tag="OQL90_001144"
     CDS             31210..32103
                     /gene="nadC"
                     /locus_tag="OQL90_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1367996.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32306..>32379
                     /locus_tag="OQL90_001145"
     CDS             32306..>32379
                     /locus_tag="OQL90_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1367997.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSA"
BASE COUNT         7805 a         8800 c         7981 g         7793 t
ORIGIN      
        1 gccacgctac aacaggatgg aactatacca atagaagctg gtgagtttaa agccaccagt
       61 actttccagg taacctaccc ttaataagtc caccgcaccg ccatcatctg gcggtgcggt
      121 aattgataaa tcattgcccc caccaatgtg actgataaaa tgccaggcaa cacgacatca
      181 tttatcagga cacgttatga aaccgaccac cattgcttca cttcagaaat gtaagcagga
      241 taaaaagcgt ttcgcgacca tcaccgctta cgactacagc ttcgcgaaac tatttgctga
      301 tgaaggactt aacgtcatgc tggtaggcga ctcgctgggc atgacggttc agggtcacga
      361 ctccaccctg ccggtgaccg ttgccgatat cgcctaccac actgcctccg tacgtcgtgg
      421 tgcgccaaac tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
      481 agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc aatatggtca aaattgaggg
      541 cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg
      601 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
      661 cggcgatgaa gcgggcgatc aactgctcag cgatgcatta gccttagaag ccgctggggc
      721 acagctgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcact
      781 ggcgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
      841 gcacgacgcc ttcggcatta ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc
      901 cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
      961 ttatccgggc gaagaacaca gtttccatta aggagtcacg ttgtgttaat tatcgaaacc
     1021 ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
     1081 gtgcctacta tgggtaatct gcacgatggc catatgaagc tggtcgacga agccaaagcc
     1141 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggaa
     1201 gatctggcac gttatccacg gaccttgcag gaggactgcg agaagctaaa caaacgtaaa
     1261 gtggatttag ttttcgcccc ttcggtaaaa gagatctacc cgaacggtac tgaaacccat
     1321 acttacgttg acgttcccgg gctttcgacc atgctggaag gcgcgagccg tccggggcat
     1381 tttcgcggcg tttcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc
     1441 tgcttcggtg aaaaggactt tcagcaactg gcgctaatcc gcaaaatggt tgccgatatg
     1501 ggcttcgata ttgaaattgt cggcgtcccg attatgcgcg ccaaagatgg ccttgcgctc
     1561 agttcccgta atggttatct gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa
     1621 gttttaagtt caattgccga caaattgcag gctggcgagc gggatcttga tgaaattatt
     1681 gccattgcag ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc
     1741 gatgccgaca cgttgctgga agtctctgaa accagcaaac gagctgtaat tctggtagcc
     1801 gcctggcttg gcgatgcccg cctgatcgac aacaaaatcg tcgaactggc gtaatgcttt
     1861 tactctaaat aattcaagtt gcaggaaggc ggcaagcgag tgacaaattc gtcaggaacg
     1921 aatttgccca gccgcaggct ggccttcggt gagagacatg gatgtctctc attaatcccc
     1981 aggagcttac ataagtaagt gactggggtt agcgaacgca gacacagcac atgcaattta
     2041 aagtatgacg agtataactg gcgctacggc tgatggcgcc aattattaat ttgccccacg
     2101 tcatggcgat ggcgtctgta agcaattatt cataacgcag acaacttagt taatcagcct
     2161 atcaatctca gcaagagata aatttgtcat ctctgcaacg tcttcccgag acatcccttt
     2221 actcagaaaa cgctgggcga attcttgcct ttcctcctgt cttccctgct gaatcccctt
     2281 ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagactccc ctcccgtttc
     2341 cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat
     2401 atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa tcagcgtgac
     2461 cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag
     2521 aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag
     2581 cgggaaagga ctgttataga cgcgtcgtgc cagctccggc gagtaaaata tatcaaacca
     2641 gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg gcaccaccag
     2701 cggcagctta tcgtgatccg cctccagatg ccggtgcatg gcagcaatag aataacgcat
     2761 catgcgaaag gccattttct tatccggctt gccttggtgt tcaatcacaa catgcagata
     2821 accgggattg ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc ctttcaggct
     2881 ctcttcaatg aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttctcgtaa
     2941 ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcgtgca ttaaaaattg
     3001 tttaaacact gcatcatgcg gtgtggtact cggtgcatcc atcttccctg ccttccgtgt
     3061 tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc gtcagccacc tctttagtgt
     3121 ttaccgagca gcgttcagag aaatttcaat tggttacagc cgttacatgg cacgcttcgg
     3181 cacggtacgc aaactgctga tgtaaatgcc gtcagatgag tagacactaa acaaaaatcg
     3241 ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc tgcttcgtta acgacagggt
     3301 agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg
     3361 actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca
     3421 gccggtattc tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc
     3481 tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg
     3541 gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat
     3601 gaagaagctc gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa
     3661 cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact
     3721 caccagccgc gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa
     3781 caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc
     3841 ggcatcgttt gctgccttca cggctttgtc attaaatccg ccaaacggat acgaaagata
     3901 ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat caaacagaat
     3961 attgtgttca ctacggctcg gtaatatggg gcggcgataa ccatccaccc gatgcaaaaa
     4021 atgggtatgt gactggaaat caaaaacatc gcgaatttcg ttaagttcag aaacgctcat
     4081 aaattgcagc gattttgggt cccacttctg cgggtgacgt ttgatgcgcg aggtaacaat
     4141 aaacgccgtc gccttcatgc catattgttt caacacggga tacgcatagc ggctcaccga
     4201 tttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga tcttattctt
     4261 cacgtaacct tccagctgca ccatgctcag tgtcgcgtat cccctgtcgc gcagccaggc
     4321 catctggtta ttgaaagcgc gtactgaggt ggtcgtcgaa gtatggcgaa aacgggtgtt
     4381 ttcttcatcg cgcagaatat ggtgataggt tagcaccggc aggccattat cgggttgggc
     4441 atccagcgcg ctgatatagg ccagtcgatc gccaatacgg atctgatacc aggtttgatt
     4501 taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccata
     4561 atggcgcact gcccgcactc ggcgcgttat agaccggcgt atctttccag gtaattaagt
     4621 tctgattact cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct
     4681 gaactggctc gagataccct ttatcgataa accctttacc aaagccaaaa ttaaatgcgt
     4741 aataacttgc ggcagtgggc tccacggcaa tgatttgccc ggcccgaata ttccccacgg
     4801 tcaccatctt gtcgccaatt tgcgcccaga tcgcagcatt ttcgatggtt tgcatataac
     4861 gggcaggtaa cgcggcacta acgcttgcgg taaacagcat cagcaggatg aataacagct
     4921 tgtttataca tgaaaaaata accgagggtg aaacaacgct tgtattgtag caaaagcgcc
     4981 atcagaatcg cgggttaata ctcatacaaa ttatgtttga ggacaaaagg cggatttttt
     5041 tgttgttggt gccaaagact actcacctcc ggcgcaccca gttcgacaat acgctcgcga
     5101 aactctgaac tggtcatggt ttcacggcaa gccatcatct gttcaattaa cggtaacgtg
     5161 acaccagaaa tcacttcaca acgggagtgt ttatggctta ataatgaagc cacgcggtac
     5221 ggtggcgctc cggctatatc ggttaagaag atgacaccct caccagagtc cgcttcatgc
     5281 agagcatcgc acatcatgcg gctgagcata ttagagctta atccgcgcca gaaattcacg
     5341 gcccggcact gaagaagtgc cccatgtttt ttctccagcg catccagtat ctcttgcgcc
     5401 cggtcatcgt gacaggtaat tacccaacct aacatcgcct tcctccttag ccgaaaatca
     5461 gtctatcctg accattgttg ataaagggtg atagccgtca aaactcctct cccccgaatc
     5521 cagaggagag gaaatagcct tagctacgca aaccacgtcc acgttggatc agcgaccaac
     5581 agatcaaata aaacaccaca ataaagacca ccagtacgcc aaaggtagtg gccagcggca
     5641 catcatttat accgaggaag ccgtagcgga aaccactgat catataaacg attgggttca
     5701 ggtgcgacag cccttgccag aacggcggca acaaagtcag ggagtaaaac accccgccca
     5761 aatacgtgag tggcgttaac acaaaggttg gcaccaggct gatgtcatcg aacgttttgg
     5821 caaacacgcc gttcagcaaa cccgcaaggg agaatagcac cgccgtgagc accagggtta
     5881 aggcaacgaa tacccacgaa tgcacctgaa acggcacaaa aaacagtgaa attgccgtca
     5941 ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc gacatatccg gcaataatga
     6001 cgtgagtcgg aaccggcgct accagcagct cttcaatatt acgctggaac ttggcaccaa
     6061 aaaatgatga cgcgacgtta gcgtaggcat tggtgatcac cgacatcatg atcagccccg
     6121 gtacgatgaa ctgcatatag ctgaagccat gcatatcgcc aatacgcgaa cctatcaggt
     6181 taccgaagat aataaagtaa agggtcatgg tgatgactgg cggcaccagc gtctgcaccc
     6241 agatacgcat aaagcgatgg atctctttcg cccagatgct ttttagcgcc acccagtaaa
     6301 gatgcatcat gcgcgatctc cttgtttttc attaaccagt gaaacaaaca gctcttccag
     6361 acggttagct ttgttacgca tacttaatac ctgaatgccc tgctcactta actgcgtaaa
     6421 tacgctgttg atcccctgct cacgcagcac ttcaacttcc agcgtcgcgg tatcgaccag
     6481 tcgatactga tagccatcga gcttcggtaa cgggcttttc ggtgcgagat cgagaataaa
     6541 ggtttccaat ttcagcttcg ccagcagcgc cttcatcgag gtattttcca ccagctcacc
     6601 gtgttgaata atgccgatat tgcggcacag catttctgct tcttccaggt agtgtgtagt
     6661 gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa aagccccaca ttgagcggcg
     6721 aagttcaata tccacgcctg cggtcggttc gtcgagaatc agtagtttag gttcatgcat
     6781 taacgcacgg gcaatcatta aacggcgctt catcccgcca gataacatac gcgcacgttc
     6841 gttgcgtttt ccccataaat cgagttgttt gagatatttt tcgctgcgga tgtacgcttc
     6901 tttgcgctcc acgccgtagt accctgcctg attcaccaca atttgctgca cggtttcaaa
     6961 cgggttgaag ttaaattcct gcggcaccag tcccaactga cgtttagcgt tcacgacatc
     7021 cttctcgaga tcgtaaccaa atacgctgac ccgcccggag gttttattta ccagagagct
     7081 gataataccg atagtggtcg atttcccggc cccgttcggc ccgagaagcg cataaaaatc
     7141 acccgcttcg acctgcaaat ctattccacg aagcgcctga acgccgcctg gataggtttt
     7201 tttaagctgt tgaagttcca gtgcaatggt cataaatttt tacttacctt acgttcttac
     7261 actttatctg tggtttaaat cgtcccggag ttgccctata ttagccaaac gtaattattt
     7321 ggttacaggt cgttaacctc catgaaagac atagatacac tcatcagcaa caatgcacta
     7381 tggtcaaaaa tgctggtgga agaggatccc gggttttttg agaaactggc acaagcgcaa
     7441 aaaccgcact ttctatggat tggatgttcc gacagtcgcg ttcctgcaga acgtttaacc
     7501 ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg ctaacctggt cattcacacc
     7561 gacctgaact gcctttccgt ggttcagtat gcagtggatg tactagaagt tgaacacatt
     7621 attatctgtg gccactacgg ttgcggcggc gtacaagccg cagttgaaaa cccggaactg
     7681 gggcttatca acaactggct gctgcatatc cgcgatatct ggttcaaaca tagctcattg
     7741 ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt gtgaactgaa cgtcatggaa
     7801 caggtgtata acctaggcca ctccaccatt atgcaatcag cgtggaaacg cgggcagaaa
     7861 gttaccattc acggctgggc ctatggcatt cacgacggct tgctgcgtga tctggatgtt
     7921 actgccacca accgcgaaac ccttgagcaa cgttaccgtc acgggatttc caacctcaag
     7981 ctgaaacacg ccaaccacaa ataaaaatgc catgccggat gcaacacatc cggcaacttc
     8041 acacttactc gtccagcaga atcactttgc cgatatacgg cagatgacgg taacgctgtg
     8101 cgtaatcaat gccgtaaccc accacaaact catccgggat cgagaaaccg ataaattcta
     8161 ccgggacgtt cacttcacga cgggacggtt tatccagcag cgtacaaatc gccagcgact
     8221 tcggttcgcg caggcttaag atctcacgca ctttcgacag tgtattcccc gagtcgatga
     8281 tatcttcaac aatcagcacg tccttgccac ggatatcttc atccagatct ttgaggattt
     8341 tcacatcacg ggtggtggac atgccgctac cgtagctgga ggcggtcata aagtcgactt
     8401 catgagatac ctgaacttca cggcacaggt ccgccataaa cataaatgag ccacgcagca
     8461 gacccaccag caccatatcg ctgccgctgt ctttgtaacg ctcagtaatc tgacgaccca
     8521 gttcggcgat acgcgcttta atctccgctt cggggatcat tacttctaca gtatgtttca
     8581 tatctctaac catatgattt aaaaacaaat cacttcgcac gggcaggttt tggcaccgca
     8641 acaaagcgca agacacgcag tataccagta aaactattca ttcgcggaat ctgttaataa
     8701 agctcgaatt gtgatgacga tcacacatgt taaaacctat aattaattgc tactaaaata
     8761 ttaatgaatt gaaatggtgt ctctttatgg caattaacaa tacaggctcg cgacgattac
     8821 tcgtcacgct aacagtcctt tttgcagcgc tttgcgggct gtatctactc attggcggag
     8881 gctggctggt cgcgattggc ggctcctggt actaccctat cgctggcctt gtgatgctcg
     8941 gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct ttggctatac gccgccctgc
     9001 tgctcggcac catgatttgg ggcgtctggg aagttggttt cgacttctgg gcgctgactc
     9061 cgcgcagcga tattctggtc ttcttcggca tctggctgat cctgccgttt gtctggcgtc
     9121 gcctggtcat tcctgccagc ggcgcagttg ccgcactggt ggtggcactg ctgattagcg
     9181 gtagtatcct gacctgggcc ggatttaacg atccgcagga aatcaacggc accttaagcg
     9241 ccgatgccac acctacggaa gctatctcac ccgttgccga tcaggactgg cctgcctatg
     9301 gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca aattaacgcc gataacgtcc
     9361 ataatctgaa agaagcctgg gtgttccgta ctggcgatgt gaagcagccg aacgatccgg
     9421 gcgaaatcac caatgaagtg acgccgatta aagtgggcga caccctttac ctgtgtaccg
     9481 ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa agagaaatgg cattacgatc
     9541 ctaagctgaa aactaacgag tctttccagc acgtaacctg ccgtggcgtc tcttatcatg
     9601 aagccaaagc agaaacggct tcgccggaag tgatggcgga ttgcccgcgt cgtatcattc
     9661 ttccggtcaa tgatggtcga ctgattgcga ttaacgctga aaacggcaaa ctgtgcgaaa
     9721 ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat gccagacacc aaaccgggtc
     9781 tgtatgaacc gacttcgcca ccgattatca ccgataaaac catcgtgatg gcaggttcag
     9841 ttaccgataa cttctcaacc cgcgaaacgt ctggcgtgat ccgtggtttt gatgtcaaca
     9901 ccggggagct gctgtgggct tttgatcccg gcgcgaaaga tccgaacgca atcccgtctg
     9961 acgaacacac ctttaccttt aactcgccaa actcttgggc accagcggcc tatgacgcga
    10021 agctggatct ggtctatctg ccgatgggcg tgaccacgcc ggatatctgg ggcggtaacc
    10081 gcacaccgga acaggaacgt tatgccagct cgattctggc gctgaatgcc actaccggga
    10141 aactggcgtg gagctaccag accgttcacc acgacctgtg ggacatggat cttccggcac
    10201 agccgacgct ggcggacatc accgttaatg gtcagaaagt gccagttatt tacgctccgg
    10261 cgaaaaccgg caacattttt gtgctcgatc gtcgcaatgg cgaactggtg gttccggccc
    10321 cggaaaaacc ggtgccgcaa ggtgctgcga aaggcgatta cgttactccg acccagccgt
    10381 tctctgaact gagcttccgt ccgaagaaag atttgagcgg tgcggatatg tggggagcca
    10441 ccatgtttga ccaactggtg tgccgcgtga tgttccacca gatgcgctat gaaggcattt
    10501 tcacgccgcc atctgaacag ggcacgctgg tcttcccggg taacctggga atgtttgaat
    10561 ggggcgggat ttccgttgat ccaaatcgtg aagtggcaat tgccaaccca atggcactgc
    10621 cgtttgtttc gaaactgatc ccacgcggtc cgggtaaccc gatggagcag ccgaaagatg
    10681 ccaaaggcac gggtacggaa tccggcattc agccacagta cggtgtaccg tatggtgtca
    10741 cgctcaaccc gttcctctca ccgtttggtc tgccgtgtaa acagccagca tggggttata
    10801 tctcggcgct ggatctgaaa accaatgaag tggtgtggaa gaaacgtatt ggtacgccgc
    10861 aggacagtat gccgttcccg atgcctgtac cggtgccgtt caatatgggt atgccgatgc
    10921 tgggcgggcc aatctccacg gcgggtaacg tgctgtttat cgccgctacg gcagataact
    10981 acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg gcagggtcgt ttaccagcgg
    11041 gtggtcaggc gacgccaatg acctatgaag tgaatggtaa gcagtatgtg gtgatctccg
    11101 caggcggtca cggttcattt ggtacgaaga tgggcgacta tattgtggct tatgccctgc
    11161 cggatgatgt gaaataacca taatgcccgg agagatccgg gcatatttcc gaatacggtc
    11221 tttttatacc gtaaacccta acatcatccc cgtatcttca tgctccagca gatggcagtg
    11281 cgccatataa gcatgttctt tcggtgcgtc gtgattaaac ttcaccagca cttcactgac
    11341 attgccttct accttaacgg tatctttcca gcccgcgcga tgagccgctg gcggtttgcc
    11401 attttctgac aagatacgga actgcgtgcc gtgaatatgg aacggatgca gcatcatgtc
    11461 gcccacgcca gagataaccc aacgttcgta ctgccctttc gccgccgcaa acatcggctt
    11521 attcatatca aacgcctgac cgttgatttt attggcatgg tggaaatcga acttcccgcc
    11581 gtggttcata tgattcatat taccgtgccc catatggccc atcatctggc tgtgatccat
    11641 tccggccatc gcctgatcgc catatttctc cattagcatc tgcataccca tcatatcgag
    11701 catcgggtcc atagaaagtt gcagcttgcg taccgtcagc ccttccagcg aaggtaacgc
    11761 aggcaggcta cttaatgtgt ctggcaaagc accggaggca ctaatagcaa tcggctgaat
    11821 ccgcattacc ggatgcggct tgtcaaacgg cgcaatcgcc atccccatct ggctgaccgg
    11881 tagcgtcacc aggtcaaagg gtttgttgtc gttaacctcc accagcactt caaaacgctc
    11941 gcccatcagc accggcagct cgctcacctt caccggttca ggtagcagac caccgtcgct
    12001 ggcaatcaca tacagcgggc gattgtcgct ggtggcgaaa ttgagcgagc gggcattaca
    12061 gccattgagc aaacgcaggc gcagccaacc acgcggggca gcgtgttgtg ggtagattgc
    12121 accgttggtc agcagcgtat cgccaaacca gcccacggcg gcggtcatca catccagttg
    12181 ataatcaatc tgcccatcag cgttaaattt tttatcctga acaatcaccg gaacatcatc
    12241 gataccccac tgttttggca gcattaattt caggatctcg tcatcttcaa ttaccaccag
    12301 cccagccaac cccatcgcca cctgtcgccc agtcttgcca tgttgatgtg gatggaacca
    12361 gcaagtagcg gcaggttgat caacgttcaa cgtcaccgag cgcttgccac ctggcggaat
    12421 aattccctgc gggccgccgt cgacttcacc cggtacttcc agcccgtgcc agtgcaacgt
    12481 cgtctcttcc gtcagttggt tatagatatc aaccgttacc gctttgccgc gctgtaattt
    12541 caccgccggc cccagcagat tgccgttata gccccaggta gttgcagttt tctcgccaaa
    12601 ggtagactga cctgcgccaa tagttaactg aatgcgatta cgggcatcgg tcgtgagcaa
    12661 atcagggatc ggtaacgttg ggcgttccgc cgcaaagact gcgcggctcc acagcggcaa
    12721 ggctgaagcc acacccagcg cgacggaata ttttaagaaa tcacgacgtt gcatagttat
    12781 ttccttattc ttaagcaggc gaaacaaaat caaacgttga gcatagtcct tccccttacg
    12841 ggaaggtcaa gccgcagaca caatttaatc gacaataatg gtcgtcgcct cgctcgcagt
    12901 gtgctaacgt ttatcttctt taagcccctg gtagaagcaa tgaagacgtt tttcagaaca
    12961 gtgttattcg gcagcctgat ggccgtttgc gcaaacagtt acgcgctcag cgagtctgaa
    13021 gccgaagata tggccgattt aacggcagtt tttgtctttc tgaagaacga ttgtggttac
    13081 cagaacttac ctaacgggca aattcgtcgc gcactggtct ttttcgccca gcaaaaccag
    13141 tgggatctca gtaattacga caccttcgac atgaaagccc tcggtgaaga cagctaccgc
    13201 gatctcagcg gcattggcat tcccgtcgct aaaaagtgca aagccttggc ccgcgattcc
    13261 ttaagcctgc ttgcctacgt caaataatcc ctgatacctt tttgtagaaa tattgaccgt
    13321 gcatccgcgg tcaatgttag ctattatgtt gcgccctttt tttacgggtg ttaacaaagg
    13381 aggtatcaac ccatggccga aaaaaaacag tggcatgaaa cgctacacga ccagtttggg
    13441 cagtactttg cggtagataa cgttctgtat catgaaaaga ccgatcgcca agatctgatc
    13501 atttttgaga acgctgcatt tggtcgcgta atggcgctgg atggcgtagt acaaaccacc
    13561 gagcgcgacg agtttatcta tcatgagatg atgacccatg ttccgctact ggcccacggt
    13621 cacgcgaaac atgtgctgat tatcggcggc ggcgacggtg ccatgctgcg tgaagtaacc
    13681 cgacataaaa acgttgagtc aatcacgatg gtggaaatcg atgcgggtgt cgtgtcgttc
    13741 tgccgtcagt atctgcccaa ccataacgcc ggtagctacg acgatccgcg ctttaagctg
    13801 gtgatcgacg atggcgtcaa tttcgttaat caaaccagcc agacctttga tgtcattatc
    13861 tccgactgca ccgatcctat cggtcccggc gaaagtctgt tcacctcagc attttatgaa
    13921 ggctgcaaac gttgcctgaa tcctagcggt atcttcgtcg cacaaaacgg cgtctgcttt
    13981 ttacagcagg aagaagccat cgacagccat cgcaaactca gccattactt cagcgacgtt
    14041 ggcttttatc aggcggcgat cccgacctat tacggcggta tcatgacttt tgcatgggcg
    14101 acagataacg acgccttacg ccatctctca accgaaatta ttcaggcgcg ttttctcgct
    14161 tctggcctga aatgccgtta ttacaatccg gcaatccata cggcagcttt tgccttacct
    14221 cagtatctgc aagatgcact ggcttcacag ccgtcctaag gagaagataa gaaattgaaa
    14281 aaactgaaac tgcatggctt taataatctg accaaaagtc tgagtttttg tatttacgat
    14341 atctgctacg ccaaaactgc cgaagagcgc gacggttata ttgcttatat cgatgaactc
    14401 tataatgcca accgtctgac cgaaatcctg tcagaaacct gttccattat cggggccaat
    14461 attcttaaca tcgcccgcca ggattacgaa ccacagggtg ccagcgtcac tattctggtg
    14521 agtgaagaac cggttgaccc gaaactcatc gacaaaacag aacaccccgg cccactgcca
    14581 gaaacggtcg ttgcccatct cgataaaagt catatttgcg tacataccta cccggaaagc
    14641 catcctgaag gtggtttatg taccttccgc gccgatattg aagtctctac ctgcggcgtg
    14701 atttctccgc tgaaggcgct gaattacctg atccaccagc ttgagtccga tatcgtaacc
    14761 attgattatc gcgtgcgcgg ttttacccgc gacattaacg gtatgaagca ctttatcgac
    14821 catgagatta attcgattca gaactttatg tctgacgata tgaaggcgct gtatgacatg
    14881 gtggatgtaa acgtctatca ggaaaatatc ttccatacca agatgttgct taaagagttc
    14941 gaccttaagc actacatgtt ccacaccaaa ccggaagatt taaccgacag cgagcgccag
    15001 gaaattaccg ctgcgctgtg gaaagaaatg cgcgagattt attacgggcg caatatgcca
    15061 gctgtttaac ggctctggcg gagctccagg ctccgccaga tttatttact tctgctgcac
    15121 gaaattgcgg taagccgcca cgacctgcag aaaatcctca acgccgcata gcgacaggct
    15181 ttcttcgtcg tagtagttca tcccctcttc catttcatcg ccagcgaatt ccagttgatt
    15241 ggcgcgaacc atcacctctt caccgtccat ccacagggtg tattcatgcc ctgcccgttg
    15301 ccaggaccgt tcgctacctt tcagtgcgtg cgctgcttgt tccacttcat caagcaaggc
    15361 caggttttct ttcacctctt cattaaacca gtgcccgacc acttcatgcc ccatggacat
    15421 acgcaccttt accactccgg taatatcgcg cagaaattcg taatccatag tgtgttcctc
    15481 tgctcccggc aatgcgccga aatcgcattg cgccattgat gtaattatcg cagccttacc
    15541 ggagaaaaac agcggaacgg cgatggttta gaaataaaaa atgcccggga atctcattca
    15601 ccgggcattg tgtcgtttat gcgcagcgcg tgcgcttact tattacaccg cagtctggaa
    15661 aatcacccca tcggcttttt cggtgtactg agaaagttgg ttgaagttca gataacgata
    15721 agtatcaacg gcagttttat ctacttgcgc aacgtaggtc tggtactctt ccggcgtcgg
    15781 cagtttgcca atcagcgccg caacagccgc cagttctgca gaagccagga agacattcgc
    15841 gccagtcccc agacggttcg ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc
    15901 tgctacacgc gcctggttac ccatacacag agaacagccc gggatctcga tacgagcccc
    15961 gctcttaccg aagacgctgt agtagccttc ttcagtcaac tgtgcggcgt ccatacgggt
    16021 tggcggtgcc acccacaggc gggtcggcaa ctggccttta tgcgcatcca gcagtttacc
    16081 cgcagcacgg aagtgaccga tgttagtcat gcaagaaccg ataaatactt cgtcgatctt
    16141 ctcgccctgt accgcagaca gcggacgcgc gtcatccggg tcgttcggag cacacaggat
    16201 tggctcttta atatccgcca gatcgatgtc aatcactgcc gcgtattccg catctgcatc
    16261 ggcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac gacgttccag
    16321 ggtacgacga tcgccgtaac cttccgcgat catccacttc agcaggacga tattagagtt
    16381 aaggtattcg atgattggtt ctttgttcag cttgatagta caaccagcgg cagaacgctc
    16441 agcggacgca tcggtaagct caaaggcctg ctcaactttc agatccggca gaccttcaat
    16501 ttccagaatg cggccagaga agatgttttt cttgcctttc ttctcaacgg tcagcagacc
    16561 ttgtttgatg gcatacagcg gaatggcgtg taccagatcg cgcagggtga tgcccggctg
    16621 cattttgcct ttgaagcgca ccagaacgga ttccggcata tccagcggca ttacgcctgt
    16681 tgcggctgca aatgccacca gaccagaacc cgccgggaaa gagataccga tcgggaaacg
    16741 ggtatgggag tcacctccgg taccgacggt atccggcagc agcatacggt tcagccagga
    16801 gtggattacg ccgtcacccg gacgcagcga cacaccgcca cggttcataa tgaagtccgg
    16861 cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt ggcagaaaga
    16921 ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt catcacgggt
    16981 catcgggccg gtagtgtcct gtgaacctac agaagtcatt ttcggttcgc agtacgcgcc
    17041 cggacgaatg cctttcacgc cacaggcacg gcctaccatt ttctgcgcca gcgagaagcc
    17101 gcgatcgctc tcagcgacat ctttcgcctg acggaacaca tcactgtgcg gcagaccaag
    17161 tgcttcacgc gctttggtgg tcaggccacg cccgataatc agcgggatac ggccgccagc
    17221 acgcacttca tcaatcagca cgtcggtttt cagttcgaag gtcgccagca gttcgcctgt
    17281 ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca atcacgtcgc ccatgttcag
    17341 gttagagacg tcgacttcga ttggcagtgc acccgcgtct tccatcgtgt taaagaagat
    17401 gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca catgtggaat
    17461 atcatcgccc ataaaccaca gaacggagtt cgtggcggat ttacgcgatg aacccgtacc
    17521 cacaacgtca ccgacgtacg ccagcgggaa acctttctgt tgcagagctt caatctgttt
    17581 gatcgggcca acaacacctg gctggtctgg ctcaatacct tcacgggcgt ttttcagcat
    17641 cgccagcgcg tgcagtggga tatccgggcg cgaccacgca tccggtgccg gagaaaggtc
    17701 atcggtgttg gtttcgccag tgactttgaa gacggtaacg gtcagttttt cagccagcgc
    17761 cgggcgattc ataaaccatt cggcatccgc ccaggactgc attacctgct tcgcatattc
    17821 gttgcctgct ttcgctttct cttctacgtc atagaagtta tcgaacatca gcagtgtgtg
    17881 agaaagtgct ttggcagcga tcggtgccag tttggcatca tccagcgcgt cgatcagcgg
    17941 atgaatgttg taaccaccct gcatggtgcc cagcagttcg atggcttttt ccggagtcag
    18001 cagaggggat ttggcttcgc ctttcgcgat agcagccagt aagcctgctt tgacataggc
    18061 ggcttcatcg acgccagggg gaacacggtt ggttaacaga tctaacagga attcttcttc
    18121 gcccgcgggc gggtttttca gcagctctac aagtgcggcc atttggtttg catccagggg
    18181 tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt cttctagcac
    18241 gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca cgctcctgtg
    18301 agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc aggattttga
    18361 cgacgtgtta atctgtttac aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg
    18421 gtctagagat tgtttccctc cgtaaaaact tcgttaattc gcatggtgat aatcaccttt
    18481 ctcaacgaat cccaagatta gtcaaaattt aaactaccgc ctctttatac tcggattaac
    18541 agcacctgcg ggtgacagtt cgccgaccat tgcgatttcc ttgagatccg aattatgaaa
    18601 atgactttgc cgtttaaacc ccatgtgctg gcactaattt gcagtgccgg gctttgtgcc
    18661 gcctctgccg ggctatatat aaaaagccgc acagtggaag cggctgtgga aacgcaatcg
    18721 acacaactgg ctgtgtctga cgctgccgcg gttacgcttc ctgcaacggt ttccgcgcct
    18781 cccgtaacac ccgccgtcgt taaatccgca ttcagcactg cacaaataga tcaatgggtc
    18841 gcgcccgtcg cgctgtatcc cgacgcccta ctttcgcagg tgctgatggc atcaacctat
    18901 ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata atccacttaa acaaggcgat
    18961 gctgctattc aggcggtatc tgaccagccg tgggacgcca gcgttaaatc actggtggcc
    19021 tttccacaat tgatggcatt gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat
    19081 gcttttctgg cgcagccgca ggacgtgatg gactcggtac agcgattgcg gcaactggcg
    19141 caacaaaccg gttcgctgaa gtcatcaaac gaacaaaaag ttattaccac aacgaagaaa
    19201 gctgtaccgg taaaacagac agtcacggca cccgtcatac catccaatac cgttttaact
    19261 gccagccccg tcattacaga gcctgcaaca accgtcattt ccattgagcc cgccaatcct
    19321 gatgtggtct atattcccaa ctacaaccca accgtggttt acgggaactg ggccaatact
    19381 gcgtatccgc cggtttatct gccaccacca gccggagaac cgtttattga cagctttgtg
    19441 cgcggattcg gctatagcat gggtgttgct accacgtacg cactattcag cagcatcgac
    19501 tgggatgacg acgatcatga ccatcatcat catgacgatg atgattatca tcaccacgat
    19561 ggcggtcatc gtgacggtaa tggctggcaa cacaacggcg acaacatcaa tatcgacgtc
    19621 aacaatttca accgtatcac cggtgagcat cttactgata agaatatggc atggcggcac
    19681 aatccaaact accgtaatgg tgtgccctat catgatcagg atatggcaaa gcggtttcat
    19741 caaaccgatg tcaacggcgg aatgagtgcc acgcagctac ctgctccaac acgcgacagc
    19801 cagcgtcagg cggcagcaag tcagtttcag caacgaacac acgccgcccc cgtcattaca
    19861 cgagataccc aacgtcaggc agcggcacag cggtttaatg aagctgaaca ctatgggagc
    19921 tatgacgact tccgcgactt cagccgtcgc caacccctga cccagcaaca aaaggacgcc
    19981 gctcgtcagc gttatcagtc agcttctcct gagcagcgcc aggcagttcg cgagagaatg
    20041 cagactaacc cgcagatcca gcagcgaaga gaggcagcgc gtgagcgcat tcagcccgcc
    20101 tcgcctgagc agcgccaggc agtccgcgag aaaatgcaga ctaacccaca gatccagcag
    20161 cgaagagacg cagcgcgtga gcgtattcag tcagcctcgc ctgagcagcg ccaggtgttt
    20221 aaggaaaaag tacagcagcg cccactgaac caacagcaac gtgataacgc ccgccagcgt
    20281 gttcaatcag catcacctga acaacgtcag gtttttcggg agagagttca ggagagccgc
    20341 ccacaacgtc taaacgacag taaccgtact gccagattga ataacgatca acggtcagca
    20401 gtacgcgaac gtctctctga gcgcggagca aggcgactgg aaaggtaaat tacagacgta
    20461 aaaaaagcgg cgtggttagc cgctttttta attgccggat gttccggcaa acgaaaaatt
    20521 acttcttctt cgctttcggg ttcggcaggt cggtaatgct accttcgaac acttctgccg
    20581 ccaggcccac agactcgtgc agagtcgggt gcgcatggat ggtcagcgcg atgtcttcag
    20641 cgtcacaacc catttcgatt gccaggccga tttcacccag cagctcgccg ccgttggtac
    20701 cgacaatcgc accaccgatc acacggtgag attctttgtc gaaaatcagc ttggtcatac
    20761 cgtctgcgca gtcggaagcg atagcacgac cagaagcagc ccacgggaag gtggcggttt
    20821 catagctgat gcctttctct ttcgcttctt tctcagtcag acctacccat gcaacttctg
    20881 gttcggtata ggcgatagac gggataactt tcggatcgaa gtagtgtttt ttaccggcga
    20941 taacttcagc ggcaacgtga ccttcgtgaa cacctttgtg tgccagcatc ggctgaccaa
    21001 cgatatcgcc gatagcaaag atgtgcggta cgttggtacg cagctgtttg tcaacgcgga
    21061 tgaaaccacg gtcgtccact tccacgccag ctttgcctgc gtcgaggttt ttaccgttcg
    21121 gcacacgacc aatcgctacc agcacggcgt cgtaacgctg cggttcagcg ggtgcttttt
    21181 tgccttccat cgtcacataa ataccgtctt ctttcgcttc aacggcggta actttggttt
    21241 ccagcatcag gttgaatttc ttgctgatac gcttggtgaa gactttaacg atgtctttgt
    21301 cagctgccgg gataacctgg tcgaacattt caaccacgtc aatctgtgaa cccagcgcgt
    21361 ggtatacggt gcccatttcc agaccgatga taccgccacc cattaccagc aggcgttctg
    21421 gtacttcttt cagttccagc gcgtcagtgg agtcccagat acgcggatct tcatgcggaa
    21481 taaacggcag ttggatcggg cgagaacccg ctgcaatgat cgcgttgtcg aagttgatca
    21541 cggttttacc gttctcacct tcaacttcca gggtgttagc cccggtaaat ttacccagac
    21601 cgttgaccac tttgactttg cggcctttcg ccataccagc cagaccaccg gtcagctgat
    21661 tgatcacttt ctctttccag gtacgaatct tgtcaatatc ggttttcggt tcgccgaaga
    21721 cgataccgtg ttcagccagc gctttggctt cttcgataac ttttgctacg tgcagcagtg
    21781 ctttagaagg gatacagccg acgttcaggc aaacaccgcc aagggtgttg taacgttcta
    21841 cgattacggt ttccagacct aaatcagcgc aacggaaggc agcggagtaa cctgcggggc
    21901 ctgccccaag taccacgacc tgagttttga tttcagtact catcatgacc tctatatatt
    21961 tatctccggc ggtcataccc gtcgtctttc aggccgcagc ggcgttagct tcacgccctc
    22021 accccagtca cttacttatg taagcccctg gggattctgg cgcttgctac cttgctgcac
    22081 ccagaaatcc atagggtatg tgaagtaata ccctaaccac caccgggtcg ttctatccgt
    22141 cggtatttta caaaattgtt aacaattttt aaacaacaaa cggcaaccga tttgtctatt
    22201 cgctaataac ctcaatacat tcatgagatt accagaaaaa agccggccgt tgggccggct
    22261 cttttactta catcaccaga cggcgaatgt cagacagcgt gttgttaatg atggtaatga
    22321 aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa ggagagagaa atcggcagca
    22381 tcagacgcgg cacgaactct ttaccattcc acaccggctc catcgcggac ttggaaacgc
    22441 cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa gtgggtagta cccaggccgc
    22501 cgatgctgga gatggtgaag caaccgccct gcatttcgcc cgcagtcagc ttaccgtcac
    22561 gcgctttctt agaaatagtc atcagctcgc gagacagctc gatgatgcct ttcttgttga
    22621 cgtctttgaa taccggaaca accagaccgt tcggggtatc caccgccaca ccgatgttga
    22681 tgtatttctt cagggtcaga cgctgaccgt cttccgacag cgaactattg aagcgaggca
    22741 tctgctcaag agctgcagca acggctttca tgatgaagac aaccggggtg atcttcacat
    22801 ccagcttacg tttcgccgct tcttcgttct gctgtttacg gaacgcttcc agctcggtga
    22861 tatcggtttt gtcgaagtgg gtaacatgcg ggatcatcac ccagttacgg ctcaggttag
    22921 caccagaaat tttctggata cggcccagtt ccacttcttc gatttcacca aacttgctga
    22981 agtccacctt cggccacggc agcatgcctg ggataccgcc gccagtcgcc gccggagctg
    23041 cttctgcacg tttgatagct tctttcacgt aagcctgaac gtcttcgcgc aggatacgac
    23101 ctttacggcc agtgcccttc actttcgcca ggttaacacc aaactcgcgt gccagacggc
    23161 ggatcagcgg agtcgcgtga acataagcgt cgttttcagc aaattcagat ttgccttccg
    23221 ctttcgcagc tggtgctgct gccggggctt cagcttttgc tgccggtgcc ggcgctgccg
    23281 cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc aacttcgaag atcataatca
    23341 gcgagccagt tttcacttta tcgccaacgt tgactttcag ttccttcacg acgcctgcaa
    23401 acggcgccgg aacttccata gaagctttgt cgccttctac ggtgatcagt gactgttcag
    23461 cggcaacttt gtcgcccact ttcaccatca cttcagtcac ttcaacttcg tcaccgccga
    23521 tatccggaac gttaacttct ttcacgccag ctgctggtgc aggggccgct gccggagccg
    23581 cttcctgttt agccgccgga gctgccgcgc ctgcttcacc cgcgacttcg aagaccataa
    23641 tcagcgagcc ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtgccag
    23701 caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtgatc agcgactgtt
    23761 cagcttcaac tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc
    23821 cgatatccgg aacgttaacg tcttttgccg ccgcagccgc gggtgctgct gccggagctg
    23881 cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga
    23941 aaatcataat cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa
    24001 cgatacccgc ctgcggagac ggaacttcca tagaggcttt gtcgccttct acggtgatca
    24061 gcgactgttc ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt
    24121 catcagcccc gatgtccggt actttgattt cgatagccat tattctttta cctcttacgc
    24181 cagacgcggg ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac
    24241 tttcttatcg atttcgccac gtttagccag ttcgcccagc gccgcaacca cgacataaga
    24301 agcatcaact tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc
    24361 agtacccagt acgcggtagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag
    24421 tttcatatag tcggtagatg ccactgccgg agcgtcgttc atcacctgag cgatatacgg
    24481 aacgcgcgga gtttccagcg gatgaagcat gttccagcgt tcacaatcct gaccatcacg
    24541 cgccagctcg gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc
    24601 caggatctct gccgcttcac ggacgtgacg caggatagaa ccggagccca gcagctgaac
    24661 tttacctttg ctaccttcaa tggtttcgag tttgtagata cctttacgga taccttcctc
    24721 agcaccttcc ggcattgccg gcatgtggta gttttcgttc agcgtggtga tgtagtagta
    24781 aacgttctct tgtttttcac cgtacatacg ctccagaccg tcatgcataa tgacagcaac
    24841 ttcgtaagcg taagccgggt cgtaagagat acagttcgga atagtcagcg actgaatgtg
    24901 gctgtgacca tcttcatgct gcagaccttc tccgttcagg gtggtacgac cggaagtacc
    24961 gccgatcagg aagccacgcg cttgctggtc gccagccgcc cagcacagat cgccaatacg
    25021 ctggaagccg aacatcgagt aatagatgta gaacgggatc atcggcagat tgttggtgct
    25081 gtaagaggtc gccgctgcca gccaggaaca acctgcgccc agctcgttga tcccttcctg
    25141 cagaatctga cctttctcgt cttctttata gtaagcaacc tgctcgcggt cctgcggggt
    25201 gtactgctga ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa
    25261 agtacgcgct tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag
    25321 cattacgttc agggcacgaa cgaaagcgat agtggtagag atctctttgc tctgctcttc
    25381 cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg
    25441 acggcttggc agataaccgt gcagtttctg acgctgtgcg tgcagatagg tatgctcttc
    25501 agaaccttcc gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac
    25561 attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc attttcttaa cctggtgcgc
    25621 gatgttttta ccttcagccg cgtcgcccat gccgtaacct ttaatggaat gagcaaggat
    25681 tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg
    25741 atcgtgacca ccacggttca gtgcccagat ctgctcgtca gtccagtctg caaccagtgc
    25801 tgcggtttca ggatatttac cgaaaaagtg ttcacgaacg tacgcaccat ctttcgattt
    25861 gaaggtctgg tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt
    25921 atctttacgc agcagttcat cccaacggct accccacatc actttgatca cgttccagcc
    25981 agcaccttcg aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc
    26041 gtcaagacgc tgcaggttac agttgataac gaagaccagg ttatccagtt tttcacgggt
    26101 agcgatggtg atcgcacctt tggattccgg ttcgtccatt tcaccgtcgc cgaggaacgc
    26161 gtaaacggtc tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt
    26221 agcctggtaa atagcaccaa tcggccccag ccccatagat acggtcggga actgccagaa
    26281 ttccggcatc agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg
    26341 gaagttatcc agctgctcct gagtcagacg accttccagg aaagcacgag cgtacacgcc
    26401 cggggagatg tggccctgga agtaaaccag gtcgccgcca tcctgctcgt tgcgtgcacg
    26461 gaagaagtgg ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatatg
    26521 gccgcccagt tcgaggtctt ttttcgacgc acgcagcacc gtcatgatgg cgttccagcg
    26581 gatagctgaa cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc
    26641 aacggggatg gtgttgatgt agttgctgat acctgtgcct gcggctacgt ttacaccgcc
    26701 tttgcgggct tcagcaagca gttggtcgat cagatactga gcacgctcaa caccttcttc
    26761 acggatgacc gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt
    26821 tgggaaacgt tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa
    26881 cctgtcccat tgaactctcg ccggaaagct caataagaca ggttctacgt ttagttgccg
    26941 cgcttttata tgcgcttgat ttacaacatc ttctggataa tttttaccag taaaatcact
    27001 aattctttcg ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacgacttc
    27061 tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg cgatgcttcg cgcgcttctt
    27121 ccggcttacc ggccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca
    27181 gcatctcgcg acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg
    27241 gctccataca ccttagcaga tgaagcagaa ccacattgtg ggccgcttca gtgacagcaa
    27301 tctgatactg gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagtt
    27361 ctatggcgtg atggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg
    27421 cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgact
    27481 caggatggtc ggagagcagc tccaccagcg gatcgctgaa gctttgccat aggctgctct
    27541 ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa
    27601 tcgcctcacg caaggaggga cgggagacgt caaactgttt tgccagttcg cgttccggtg
    27661 ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa
    27721 tcacatcgga gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc
    27781 cacttgccga agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt
    27841 attcacctta tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa
    27901 cttgacatat ataacgtttc aaagttgtaa ctatgcacaa atgtagactt tacgtaggaa
    27961 agggattttt taacgattat taaactataa aaatccgaat tgaaccgatt cacttaccaa
    28021 ttttgtgatt tttaattcaa ttaaaacgaa tttaaattca ttctacatat tgagaggggt
    28081 tgaggctgag ctttacaaac ggtttctttt taagcaactc atcttcaacc atgcataaag
    28141 cgggtgcatt cgctgccgca taccattatt cttgatctga cggaagtctt tttgtaacaa
    28201 ttcaaacttc tttgatgtaa acaaattaat acaacaaacg gaattgcaaa cttacacacg
    28261 catcactgcg tagatcaaaa aaacaaccac cgcacgaggt ttcatgatgg aaggtcaaca
    28321 gcacggcgag cagctaaagc gcggccttaa aaaccgccat attcagctta tcgcgctggg
    28381 tggcgcgata gggaccgggt tattcctggg tagcgcctcc gtaatacagt ccgcagggcc
    28441 agggattatc ctgggttacg ccattgctgg ttttatcgcc tttctgatca tgcgtcagct
    28501 gggtgaaatg gtggtcgaag aacctgtcgc aggctccttt agccactttg cttataaata
    28561 ctggggcagt tttgccggtt tcgcctctgg ctggaactac tgggtactgt acgttttagt
    28621 tgccatggct gagctgactg ccgtgggtaa atacattcag ttctggtatc cggaaatccc
    28681 cacctgggtt tctgccgccg tattctttgt ggtgattaac gccatcaacc tgaccaacgt
    28741 taaagtgttt ggcgagatgg agttctggtt tgccattatc aaagttatcg ctgtggtagc
    28801 gatgatcatc ttcggcggct ggctgctatt cagtggcaac agcggcccgc aggcgaccgt
    28861 tagcaacctg tgggatcagg gtggtttcct gccgcacggc ttcaccgggc tggtgatgat
    28921 gatggcgatt atcatgttct cgttcggtgg tctggaactg gtggggatca ccgcagcaga
    28981 agctgataac ccggagcaaa gtataccgaa agcaactaac caggttatct accgcatcct
    29041 gattttctat attggttcgt tagccgttct gctctcactg atgccgtgga cccgcgttac
    29101 cgccgatacc agtccgtttg tgctgatctt ccacgagtta ggcgatacct ttgtggcgaa
    29161 tgcgctgaac atcgtggtac tgactgcggc gctctccgtg tacaacagct gcgtatattg
    29221 caacagccgt atgctgtttg gtctggcaca acagggtaat gcgccaaaag cgctggcgtc
    29281 tgtcgataaa cgtggtgtac cagtaaatac cattctggtg tctgcactgg taacggcgtt
    29341 gtgcgtactg attaactacc ttgccccaga gtccgctttc ggactgttaa tggcgctggt
    29401 ggtatctgca ctggtaatca actgggcgat gattagcctg gcgcatatga aattccgtcg
    29461 cgccaagcag gaacaaggcg tggtaactcg cttccctgct ctgctttatc cgctgggtaa
    29521 ctggatctgc ctgctgttta tggcggcggt actggtgatt atgctgatga ccccaggaat
    29581 ggcgatttcg gtatacctga tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt
    29641 taaagagaaa accgccaaag ccgtaaaagc gcattaatct ctctacgccc tcacccgtac
    29701 agggtgaggg caataatctt tacaccaacg ccccgtaaat cgtcagtagt gcaatcacca
    29761 ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc tgccttcggc gtctccacct
    29821 tatcgacatg cggttcacgc gccagagaga actgcgccag acgcgttaac acctgatact
    29881 gcgaagtatg gaagtcaccc agcgaagcaa accaggccgg taacgctttc tccccatgac
    29941 cgatcaaggc atataccaca cccgcaagac gaaccggcac ccaatccagt acatgaagca
    30001 cggcatcaat gccggactgt aaacgatgat gcggcgtctg gtatcgcgcc agccagtatt
    30061 gccatgcacg caaaaacgca taccccatca gcgtaacggg tccccagatt ccccccacaa
    30121 tcaaccagaa cagcggagca agataaaaac gaaagttaat ccacagcaat gcattttgca
    30181 gctcacgcaa atactcacgt tcatcgcagc ctgccgggac gccgtgaatc atggtgagtt
    30241 cgccagccat cgtgacacgg gcatggctat cattacgtga agcagctgtc agataagcat
    30301 gataatgaag acgaacttta cctgcgccaa tacacagcaa accaatcagc agccacacca
    30361 gcagcgtggg aacgttgaac aatactccct gcaatgcgcg taacagtaaa aaagtcacgc
    30421 ccatcgcaat aatggtcatg cctaacgtgc gcccgagaga aaaatgtttc acccgccgaa
    30481 agaacgcttc aagacgatga tcaagctgcc agtgctcgcc caacttaaac aggcgctcga
    30541 aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc tccttgctga ccagcgcacg
    30601 aaaccgcgcc caatcaaatg cagggccggg atcggttttc cgatccggcg caatatcaca
    30661 atggcccgtc atgttattag cgatatctgg atagcgatca atcagtgccc gcgtaaccgc
    30721 cgcaagctgt tgatactgcg catcggtata cgccagcgta tcggtgcctt caagctcaat
    30781 cccaatagaa aaatcattgc agcgttcgcg cccctgatac tgagagactc ccgcatgcca
    30841 tgcacgttta tcgaaaggaa catactggac tatttcacca tcacggcgaa tcaaacagtg
    30901 agcggagacg cgcaaatggg cgatctcagc aaagaaagga tgtgcctgcg gatcaatagt
    30961 tccagtgaat aatgcgtcga tccacggacc gccaaactcg cctggcggca ggctaatatt
    31021 gtgcaccacc agcagggtgg gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg
    31081 aacgcggcgc gcgccaacca gccacccctg ttctaacaac atgcaggatc tccttatatg
    31141 tggtgctaat acccggttca gagtagcatg tttctacctt atgattcgtt agctatctgg
    31201 agttttaaca tgccgcctcg ccgctataac cctgacaccc gacgtgacga gctgctggaa
    31261 cgcattaatc tcgatatccc cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga
    31321 acagtcgatg ccaacaatga cattacggca aaacttttac cggaaaactc tcgctctcat
    31381 gccacggtga tcacccgcga gaatggcgta ttttgcggca aacgctgggt tgaagaggtg
    31441 tttattcaac tggcaggcga cgatgtcacc ataatctggc atgtggatga cggcgatgtc
    31501 atcaacgcca atcaaccctt gttcgaactt gaaggcccat cccgcgtgct gttaacgggc
    31561 gaacgcactg cgcttaattt tgtgcaaacc ctttcaggag ttgccagtaa ggtacgccac
    31621 tatgtcgaat tactggaagg caccaacacg cagttgttgg atacgcgcaa aaccttacct
    31681 ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg gcggagcgaa tcaccgtctg
    31741 gggctttctg atgccttcct gatcaaagaa aatcatatta ttgcctccgg ctcagtgcgc
    31801 caggccgtcg aaaaagcgtc ctggctgcac ccggatgcgc cagtagaagt cgaagtagag
    31861 aatctggaag aacttgatga agccctgaaa gcaggagccg atatcatcat gctggataac
    31921 ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca acggcaaggc gctactggaa
    31981 gtgtctggca acgtcactga caaaacactg cgcgaatttg ccgaaacggg cgtggacttt
    32041 atctccgtcg gtgcgctaac taaacacgta caagcactcg acctttcaat gcgttttcgc
    32101 taattgctga cagatttatc cgctccatcg cgggcggata atctcccctt ccccaacttt
    32161 cttcgtaacg cctcgcaaat ttcttgtctt tcgctgcaac gaaataaaaa acctcggaag
    32221 ctgctttccc gtttcgtttt ttgcccctcg cttgtgctga tggcgtttgc caaagtagcg
    32281 ccaaccaaat caaggagcga aacagatgga caagcaacgc ggttttacac ttatcgaact
    32341 gatggtggtt attggcatca ttgccatttt aagcgccat
//
LOCUS       DAJVOY010000028        31338 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVOY010000028 DAJVOY010000000
VERSION     DAJVOY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.028"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..3268)
                     /locus_tag="OQL90_001146"
     CDS             complement(<1..3268)
                     /locus_tag="OQL90_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCS1367998.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLVIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     F"
     gene            3386..4579
                     /locus_tag="OQL90_001147"
     CDS             3386..4579
                     /locus_tag="OQL90_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCS1367999.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4643..5896)
                     /gene="glyA"
                     /locus_tag="OQL90_001148"
     CDS             complement(4643..5896)
                     /gene="glyA"
                     /locus_tag="OQL90_001148"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1368000.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6224..7414
                     /gene="hmpA"
                     /locus_tag="OQL90_001149"
     CDS             6224..7414
                     /gene="hmpA"
                     /locus_tag="OQL90_001149"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1368001.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7459..7797)
                     /gene="glnB"
                     /locus_tag="OQL90_001150"
     CDS             complement(7459..7797)
                     /gene="glnB"
                     /locus_tag="OQL90_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1368002.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7858..9192)
                     /gene="glrR"
                     /locus_tag="OQL90_001151"
     CDS             complement(7858..9192)
                     /gene="glrR"
                     /locus_tag="OQL90_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1368003.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9182..9895)
                     /gene="qseG"
                     /locus_tag="OQL90_001152"
     CDS             complement(9182..9895)
                     /gene="qseG"
                     /locus_tag="OQL90_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1368004.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10060..11487)
                     /gene="qseE"
                     /locus_tag="OQL90_001153"
     CDS             complement(10060..11487)
                     /gene="qseE"
                     /locus_tag="OQL90_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1368005.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12045..15932)
                     /gene="purL"
                     /locus_tag="OQL90_001154"
                     /gene_synonym="purI"
     CDS             complement(12045..15932)
                     /gene="purL"
                     /locus_tag="OQL90_001154"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1368006.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16193..17749
                     /gene="mltF"
                     /locus_tag="OQL90_001155"
     CDS             16193..17749
                     /gene="mltF"
                     /locus_tag="OQL90_001155"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1368007.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17746..18282)
                     /gene="tadA"
                     /locus_tag="OQL90_001156"
     CDS             complement(17746..18282)
                     /gene="tadA"
                     /locus_tag="OQL90_001156"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1368008.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18307..18942)
                     /gene="yfhb"
                     /locus_tag="OQL90_001157"
     CDS             complement(18307..18942)
                     /gene="yfhb"
                     /locus_tag="OQL90_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1368009.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19151..19999
                     /locus_tag="OQL90_001158"
     CDS             19151..19999
                     /locus_tag="OQL90_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1368010.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20055..20315
                     /gene="yfhL"
                     /locus_tag="OQL90_001159"
     CDS             20055..20315
                     /gene="yfhL"
                     /locus_tag="OQL90_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1368011.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20509..20589)
                     /gene="shoB"
                     /locus_tag="OQL90_001160"
     CDS             complement(20509..20589)
                     /gene="shoB"
                     /locus_tag="OQL90_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1368012.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21009..21389)
                     /locus_tag="OQL90_001161"
     CDS             complement(21009..21389)
                     /locus_tag="OQL90_001161"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1368013.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21389..22120)
                     /gene="pdxJ"
                     /locus_tag="OQL90_001162"
     CDS             complement(21389..22120)
                     /gene="pdxJ"
                     /locus_tag="OQL90_001162"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1368014.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22132..22860)
                     /gene="recO"
                     /locus_tag="OQL90_001163"
     CDS             complement(22132..22860)
                     /gene="recO"
                     /locus_tag="OQL90_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1368015.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22872..23777)
                     /gene="era"
                     /locus_tag="OQL90_001164"
     CDS             complement(22872..23777)
                     /gene="era"
                     /locus_tag="OQL90_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1368016.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23774..24454)
                     /gene="rnc"
                     /locus_tag="OQL90_001165"
     CDS             complement(23774..24454)
                     /gene="rnc"
                     /locus_tag="OQL90_001165"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1368017.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24727..25701)
                     /gene="lepB"
                     /locus_tag="OQL90_001166"
     CDS             complement(24727..25701)
                     /gene="lepB"
                     /locus_tag="OQL90_001166"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1368018.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25717..27516)
                     /gene="lepA"
                     /locus_tag="OQL90_001167"
     CDS             complement(25717..27516)
                     /gene="lepA"
                     /locus_tag="OQL90_001167"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1368019.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27714..28193)
                     /gene="rseC"
                     /locus_tag="OQL90_001168"
     CDS             complement(27714..28193)
                     /gene="rseC"
                     /locus_tag="OQL90_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1368020.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28190..29146)
                     /gene="rseB"
                     /locus_tag="OQL90_001169"
     CDS             complement(28190..29146)
                     /gene="rseB"
                     /locus_tag="OQL90_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1368021.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29146..29796)
                     /gene="rseA"
                     /locus_tag="OQL90_001170"
     CDS             complement(29146..29796)
                     /gene="rseA"
                     /locus_tag="OQL90_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1368022.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29829..30404)
                     /gene="rpoE"
                     /locus_tag="OQL90_001171"
     CDS             complement(29829..30404)
                     /gene="rpoE"
                     /locus_tag="OQL90_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1368023.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30401..30556)
                     /gene="rseD"
                     /locus_tag="OQL90_001172"
     CDS             complement(30401..30556)
                     /gene="rseD"
                     /locus_tag="OQL90_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1368024.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30812..>31338
                     /locus_tag="OQL90_001173"
     CDS             30812..>31338
                     /locus_tag="OQL90_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1368025.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGT"
BASE COUNT         7492 a         8398 c         7908 g         7540 t
ORIGIN      
        1 taaaaataaa aggcatcaca gtggtgaata atgccgcttt cggccaggcc ggattaatcg
       61 ccgtcaattc agcgcgagca gactcatatt gcgcaacctc cccgagtccc gcggacgcca
      121 gcatcgccac catcaggcat ttttctttat gctgctgttg taaatcgcca tacagcgata
      181 acaggtcagg ttgtgaaaca gcaaagaaat ccgcctcaat actggttttc gccatctctt
      241 gcgcccactg tttcatttca ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc
      301 gcagcgccat tccttgccag aagagataat caaccggctg atcgttgtaa taactgtgga
      361 tgttaatggt gcgatcgccg gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat
      421 cgccctgcgc gttggcgcat atcgcctgcc agaaccagat gtcgttatca gtttgccccg
      481 gtaaacggcc ttcgcttaaa ttctccggat aatgcagcgc ggcatgaagc agttcgctgg
      541 cctgctgcgg ctctctggca tcaagatgct gccaggcgcg taataactgg ttgaggataa
      601 actgactggt gaccttccct tccccgcctt cccacgggtg gaatttacgc gtggcgagaa
      661 tgtccgccgc tttgtctgcc tgacccgtga gatgccacaa attgagcagt tctgcggtca
      721 tgtcgtcgcg tttcagcgca atttccagat tattttccag acgcgccagt cgtttctccg
      781 gtgtggctcc acttaactta tcaagcaaat cccgttcgaa aagcagacgt gcatcctgcg
      841 gcgcaagctg ataagcatta tcaagataac gcgcggccag ctcataatcg tgttgcttat
      901 tccacgcatg gatcgctaac ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc
      961 aacgttgcca aaaggcaatg gctttgttgt agctacgttt gttgtagtag aagcaggcca
     1021 gtaaatggcg agcaaaccag cactcttcaa tactctccag cgccgccact tcttccagcg
     1081 tattcgggaa acggacaaac tgcgggaaga catcaatggc ttttgcgacc agttcgccac
     1141 gttcggcttt cggcagcaag ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca
     1201 gtgcgttcag catctctgcc gccagggtgg gcattcccca gttaatcagt tgcccggcgg
     1261 tcatcagggc gttaacgtcg cgtccctgac acagaccgcg ccactgcgcg agagctgatt
     1321 cgctacgacc atcgaaccag ttcagccacc acagagtggc gttcagcgga taatcgcgca
     1381 gcagtttctc gcgctgcaca cgcgcgttgt cctgacgacc acttaacacc agcagcagat
     1441 tatgcaggca aagcacttcc tgattggttg ggcaggcgcg aagactttgt tggcaaaaat
     1501 ccagaccagc gtcgaagtta ccattacgcg ccgccagtcg tgccaggcca taatagccac
     1561 cggctttgct gttgccgctc cagaccgcac gccagaaatc ctcttcggct tgttgatatt
     1621 gtccctgacg ttcgtaagca ctggcgcgaa tcaaactcgc ctgtccgcac tgcggatttt
     1681 tgttcagcgc atgtgcgcgt ttcagagcct gagtggcata cgccaccgct tgcgggaaat
     1741 cggcgcggtt atattccagc atcgccagcg ccaggttaca gcggtaatcc agcggatcca
     1801 gcgccacgcc gcgcaggtag taatcgaacg gtgaacggct ggcgtgatga tattgctcca
     1861 gatgctgacc gataaaccag gcttcatctg tactggtaat gtcttgtgct gccagtggcg
     1921 ctttggcgac gtccagcaac ggcaactctt gcggctgatg ttcctgataa ctcagtacaa
     1981 tattgccgtc ggcatcggag agctcaatgg tcagcctttc aggattgata ccgtgcaaca
     2041 cgccctggat ggcggtcgca ggcatcagtg ccacggcatc atcaagtaac gcgttgcatt
     2101 tgccgatttc gcggatcacc aggcggtatc cgttcaacgg agagatggca tacagccccc
     2161 actcaatccc ccgcttacta cgctggagtt ttatcaccgc atcgcgggag gcattttgca
     2221 ccatgcccaa agaatgataa ggcaggaaat actgctcaaa acgcttctct tcgtaagcat
     2281 caagccaggt aaaatcgggc tggttatcgg caaaaatacc ggtcatcagt tcgatatacg
     2341 ggccgttatt gtcggtcaga ctcttatccc acgcctggcc aaattcactg tgtccccaac
     2401 tccactgttt tttacctggc gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt
     2461 catcgtgaca ccacgcgccg acaaaatcgt actgcgattt ttcggccata tatgaggttg
     2521 taacaggcac atttttatag caagaaatgt ccactccggc ggagtagtcc actttgtagt
     2581 aagtgccggt ggcgatgggg aaagcggaga cggcccgttt gccgtgatca aacaccgcag
     2641 tcacatccgg tgggaaaacg ctctgatgcc cttccccccc tttcactgcc gggttggccc
     2701 accacaagaa atgacgcggc gtggcgttac cgttatagac gcggctggcg atttccagcg
     2761 ccgcccggtc agggcgcagg gtgaaacctg tcatcacctg taaaccatgc atcggctccg
     2821 tttcgcctac ccacaccgtc tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg
     2881 gcataaaggt ggtcgggcga tgatgttgcg gccagttaaa ctcaatccca ccggaaatcc
     2941 acggccccag cagccccacc agcgcaggtt taatgacttc attgtgataa acaaaatcgc
     3001 gctgtttcac tttatcccac gcgcgatgca cccgaccgcc cagttccggc aggatcatca
     3061 ctttgatgta gtcgttttcc agccacaccg cctgccagga tttcagggtt ttctgctcgc
     3121 tcagcgtatc ggtcacgccg tagggataaa ccgcgccgga cgatccctga taaacgcgat
     3181 tttccaggaa catgggatgt atatcctgcg gcccggtttc ataggtcggg atctcaacgc
     3241 gctcttgcca cacttttact ggagtcatgg tgccctcaat attaacaaga catactgaat
     3301 taaaagattt gtggcagtgt attgaacaat ctggcaatgt tttcgcggaa taatcacgca
     3361 attaactaaa caaggtttag tgaagatgag agcctgcatt aacaatcaac agattcgcca
     3421 ccataacaaa tgcgtgattc tggaactgct gtaccggcaa aagcgcgcca ataaatcaac
     3481 gctggcccgg ctggcgcaaa tttcgattcc ggcagtcagt aatattttgc aggaactgga
     3541 aagcgaaaaa cgggtggtga atattgacga tgaaagccag acgcgcgggc atagtagcgg
     3601 tacatggctg attgcgccgg aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag
     3661 tattgagtgt caggttgcta atgcttgttt aagtccgaaa ggtgaatttg agtatttaca
     3721 gattgatgca ccgacgccgc aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca
     3781 ccgtaaattg tggccggacc gcaccatcaa tctggcgctg gcaatccacg gtcaggttga
     3841 tccggtgacc ggcgtgtcgc aaaccatgcc gcaagcgccg tggacaacgc cggttgaggt
     3901 gaagtatctg ctggaagaga agctgggcat tcgggtgatg gtcgataatg actgcgtgat
     3961 gctggcgctg gcggagaaat ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa
     4021 cgttgattac ggcattggct cgtcgttcgt gattaacgag caaatttatc gcggcagttt
     4081 gtatggtagc ggacaaattg gtcacaccat cgttaatccg gatggcgtcg tctgcgactg
     4141 tggacgttac ggctgcctgg aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg
     4201 ggtatggcta aaatcacaac cggttaatac tcagcttgat cctgaaaaac tgacaaccgc
     4261 gcagttaatc gctgcctggc agagtggaga accgtggatc accagctggg ttgatcgctc
     4321 tgccaatgcc attggtttga gtctgtataa cttcctcaac atcctcaata ttaatcagat
     4381 ttggttgtac ggtcgcagtt gtgcctttgg tgagaactgg cttaatacta ttattcgcca
     4441 gacaggattt aacccgttcg accgcgacga aggaccgagc gtgaaagcga cgcaaattgg
     4501 cttcgggcaa ttaagccgcg cacaacaggt gctgggaatt ggctatttgt atgttgaggc
     4561 gcagttacga cagatttgat ggcgcgataa cgtagaaagg cttcccgaag gaagcctttt
     4621 ttcaacagat taaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa
     4681 ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca
     4741 tccagccagc cagttctttc gcttctgctt ctttgaagcc gcgacgcgta attgccggag
     4801 tacccacgcg aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt
     4861 tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt
     4921 tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc
     4981 cgcgctcgag gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag
     5041 ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca
     5101 tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt
     5161 cgctaccacc tttcgccagg atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg
     5221 tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc
     5281 ccgcaacgtg cgccatatca acgaacaggt aagcaccgat gctgtcagcg atttcacgca
     5341 ttttcgccca gtccaccacg ccggaatatg cagagaaacc accgataatc attttcggct
     5401 tgtgttcttt ggcttgtttt tccagatcgg cgtagtcgat atgaccggta gcatcgatac
     5461 cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt
     5521 gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt
     5581 agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga
     5641 acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc
     5701 cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg
     5761 cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct
     5821 gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca
     5881 tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat
     5941 aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct
     6001 tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag
     6061 agcttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca
     6121 cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg
     6181 catttgaaat acatcaatta agatgcaaaa aaaggaagac catatgcttg acgctcaaac
     6241 catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa agttaaccgc
     6301 ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt ttaacatgag
     6361 taaccagcgt aatggcgatc aacgtgaagc cctgtttaac gctattgccg cctacgccag
     6421 taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac
     6481 cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt tggcaacgct
     6541 ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag cctatggtgt
     6601 actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc
     6661 cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc gcagcgcgct
     6721 tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat accgtccggg
     6781 gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa ttcgtcagta
     6841 ctctttgact cgcaaaccgg atggcaaagg ctatcgtatt gcggtgaaac gcgaagaggg
     6901 tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt
     6961 cgctccggca ggtgatttct ttatggctgt cgcagatgac acaccagtga cgttaatctc
     7021 ttccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa aagcaggcca
     7081 cacagcacaa gtgaactggt tccatgcggc agaaaatggc gatgttcacg cctttgccga
     7141 tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct ggtatcgtca
     7201 gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga tggatttgag
     7261 caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctctgcg gcccggttgg
     7321 cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg aaaacattta
     7381 ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat ggaaacatcc
     7441 ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat
     7501 gacccgtgcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg tttgcgccgt
     7561 gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt
     7621 cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg tatggccttt
     7681 ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga cttcggccag
     7741 tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct ttttcatgct
     7801 attccttgaa aaggtcgcct gtcttttgat ctgctaaacg taacacataa cgccaattca
     7861 ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa attctgtccg
     7921 gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtaattt gcagcagctt
     7981 acgcaaatag ttgagttcaa actgattacg tgcctcaaca aaggttggca gcgccgtgtt
     8041 ttcaccctcc agcgcctgct ctactaatgc atcgctaata accggagatg aggtcagcgc
     8101 cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccagc tagcggtcat
     8161 caggcgtttc atcgcatcag tggagaacgc gcggacaaac ggtttatgtc gctctgccgc
     8221 ctggcgcaac aggtgatttg ccagaagcgg aatgtcttct gtgcgctctg ccagagccgg
     8281 aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt ccccgcgcgc
     8341 catcgctttt ggcagatcac ggtgagtggc agaaataatc cgcacattga tatcaatatc
     8401 gcggttactg cccagcgggc gcactttacg ctcctgcaac acgcgcagca gtttgacctg
     8461 taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt ccgccgcctg
     8521 gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat gaccaaacag
     8581 ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa atggtttgct
     8641 gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg
     8701 accgttaatc aaaacgctga cgtctgattg cgccaccagc cgcgcctgtt ccagcaaacg
     8761 cagcatcagc gggctgcggg tgacaattgc ctcgcgccag cgttcatcgg tggctggcgc
     8821 ggattgctcc agcgcatcgt caattgcctg atatagcgcg tctttgtcga caggcttggt
     8881 gaggaaacta aaaacgccct gctgtgttgc agcaacggca tcggggatag aaccatgcgc
     8941 agtaagaata attactggca ttcccggctg cactttctgg atttcagcaa atagctgcat
     9001 accgtccatt tcatccatcc gcaggtcgct gataactaaa tctacttttt cgcgattcag
     9061 tacccgtaat ccttcagcgc cactttccgc cgtgaccaca ctgtagcctt cgctggtcag
     9121 gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata aatgcgcagg
     9181 tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca ctctcgtgcg
     9241 gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga gagctggcgt tcaatatcgg
     9301 tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg tgatgttgct
     9361 ggcgcaatgt atccagctcg ctgtcgctgg actgctggag tttactgtaa cgttggcgct
     9421 cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac agtgggcgaa
     9481 cctgtgccgg gatctcggta cttaacgctt caatacgcgc gaccagctgg cggcgctcat
     9541 acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt tgccagcagc
     9601 cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg cattaaacga tcagcacaat
     9661 ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc agcgcccaga
     9721 tgtcgctaca ttcagtagaa agatagtcag ccagttgata aaccggaatt ttttcttctg
     9781 ctggcgtatc aatggctggc ttattgtgat tctgcacgca acccagcaat gccagacatg
     9841 gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt cgcatattca
     9901 ccagacttaa agcctatccc agtgggcgta attgttgcag acagtctgga catggacagc
     9961 gcggagaaac cggagcgtac atatcgtacg tgaagatttc gagcactgcc cggagccgaa
    10021 ctgacaaata aaatagcctg ataggatagg ctgttagcat tatttcgtgt ttttcgacga
    10081 cggtaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat acagttcccc
    10141 ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc ccttcaccgc
    10201 cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag atcatggcgc gttcctcttg
    10261 cggaatgggc gtgcctgtat tgatgacatc aatataaacc cgcgcaccat gtgaactgct
    10321 gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt aaagattatc
    10381 cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga gatcgacgtc
    10441 ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaaccactg tctccaccag
    10501 cggtgctaat tcaacattct ccagttccac cgcactgtcc gcctgtttac ggttgtaatc
    10561 aagcagttgt tcgatcagtt tttgcaaatt gcggctgctg ctatcaagaa tgctcaccac
    10621 ctctttttgc tctggcgtaa gcggcccgac aacctggtca gccagtaatt cagtgccctc
    10681 gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa attgatggcg
    10741 ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc caaccgagcg
    10801 taactcgctc ggtccactga acgaaacgct attgcccaga gaacgccctt cccccagacg
    10861 gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga aaagtagcac
    10921 catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt gcccacgttc
    10981 ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg tggcctgcac
    11041 catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag catcgggacc
    11101 actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg cctggtaaag
    11161 tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg
    11221 gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac ggtagctacg
    11281 ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa taagcgtagt
    11341 gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg cttgccatgc
    11401 cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt gtcgtaatga
    11461 gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga atagcctgat
    11521 gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt tgttatggaa
    11581 taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc tgatatcaga
    11641 agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg
    11701 ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact gtgccaataa
    11761 agaaaatagt ttggtaacgt actgattata cgttgctttg agggatttat ggcttcttcg
    11821 ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag acagcttaag ataaacttat
    11881 tagattacaa taaaatcaat cacttaagtg tcgccagaaa gcgacacggt aaaccaccca
    11941 ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg gagcttatga tagtggttgg
    12001 tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga tgaattaccc caactgctta
    12061 cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc cccagttttc cggatgccag
    12121 gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt gactcgacca
    12181 ctttcagtcg tgactgccgt aataccgttc ggtgaaccgt tcgggttggc cgggtaggtt
    12241 tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt gctttccagt
    12301 gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga gacagcaatc
    12361 ggcatttgcg agcccaccat cccctgcaac aacagagacg ggctttgggt tacttcaacc
    12421 aggctgaaac gcgcttcaaa gcgatcggag gtattgcgca caaaacgtgg ccacaactca
    12481 ctacctggga tcagttcgcg caggttagac atcatctggc aaccgttaca tacccccagc
    12541 gccagcgttt gcggacggtg gaagaaggtt gcaaactcat cgcgtacacg gtcattgaac
    12601 aggattgact tcgcccaacc ttcaccggca cccagcacat caccgtagga gaaaccaccg
    12661 cacgcgacca gggcgtggaa atcttccagg cccgtgcgtc cggtcagcag gtcactcata
    12721 tgcacgtcga tagcatcaaa gcctgcacgg tggaaagctg ccgccatttc aacatgcgag
    12781 ttcacgccct gctcacgcag tacggcaact ttcggacgtg cgccagtggc aatatacggt
    12841 gctgccacat cttcgttgat atcgaacgac agttttacat tcaggcccgg atcggcgtcg
    12901 ttagatttcg cctgatgctc ctgatcggca cactccgggt tgtcacgcag gcgctgcatc
    12961 tgccaggtag tttctgccca ccagacacgc aacgtggtgc ggctttcgct gaatacagtc
    13021 tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgccctac ataatggaca
    13081 caatcagcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc agcggcacga
    13141 acctgaatca ccgcacccag ttcttcgtta aacaacgccg ccaggcgatc gtcgcccaga
    13201 gaggcaatat ccgcgttaat gccgcaatga ccagcaaagg ccatttccgc cagcgttacc
    13261 agcaggccgc catcagagcg gtcgtggtat gcgagcagct tacgctgtgc aaccagcgcc
    13321 tgaatcgcgt catagaagcc tttcagttgc gcgacgtcgc gtacatctgc cggtttgtcg
    13381 ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt gcctttgccc
    13441 aaatcaatca gcaatagtgc gttatcttcg gtagaaagct gtggcgtgat ggtgtgacgc
    13501 acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt catttcgcgc
    13561 tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc caccgggatc
    13621 gtcaggccca gcgccggaca aagctcttcg cccacggctt taacggcttc atacaggccc
    13681 gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt aatgcgtttg
    13741 atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc cagacgggca
    13801 gaggcggcga aatccagcag cgcaaccggc gcacgctcgc caatcgccat cgcttcaccg
    13861 tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac ctgccacgga
    13921 cccaccatct gatcgcgcgc taccatgcca gttacgctgc ggtcaccgat ggtcaccagg
    13981 aaagttttct ccgccacggt cggcagatgc agtatgcgtt tcaccgcgtc tgcaatggtg
    14041 atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc gcgggtcatc
    14101 ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt atcaaaatga
    14161 cgatcgtgta gagaaagatg cagttcttcg gtcgcttcac caatcaccgc gtagggtgcg
    14221 cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac cgccagcacg
    14281 tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcctgg ctcgtcgctc
    14341 agaatgtcgc gcagttcaaa tttaccgcca cgcccgccgt cgctcaccag ttccggcata
    14401 gcgttagaaa gaccgcctgc gccaacgtcg tggataaaca gtattgggtt ggcatcacca
    14461 agctgccagc aacggtcaat cacttcctgg cagcgacgct ccatctccgg gttgtcgcgc
    14521 tgtacggaag caaagtcgag gtcggcatca gactgaccag acgccatgga agacgccgcg
    14581 ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt cgcgccaacg
    14641 ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc cgccagcatg
    14701 atcggtttgt gataaccgcg cagctcttcg ccgttgtggc tgttcacttt ttcttcataa
    14761 gtacggaagt agccgttcag tgccggacga ccaaattcgt tgttaaacgc cgcaccgccc
    14821 agcgggcctt cggtcatgat gtccagcgcg gtgacaatgc gctcaggctt accgaaatct
    14881 tcttcccacg gctgttcgaa gccaagaatt cgcaggttgg aaacggagaa accaaccaaa
    14941 cccgctttcg gcttcgcgcc gcgcccggtg gcaccttcat cgcggatttc accgccggaa
    15001 ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt ttcgactttc
    15061 atcagaatat gcgccggttc ctgatggaaa tcatagcggc ccgtttcgtg gtcagcaaag
    15121 tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc agagagaacg
    15181 tgatctggcg tggtttcgaa agtatttttg atcatcctga acagcgattt cggctgttgt
    15241 tcaccgtcga taatccagtc ggcgttaaaa attttgtggc ggcagtgctc agagttcgcc
    15301 tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt gaaagcatcc
    15361 tgcagatagt caatttcatc ttccgccaga gccaggccaa gacgcaggtt agcgtcgatc
    15421 agcgcctgac ggccctgccc cagcaaatca acgcttgtga ccggagtcgg ttgatggtgg
    15481 gcaaacaact gctctgcatc atctaaatca aaaaagacag tttccatcat gcggtcgtgc
    15541 agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc tatatagtaa
    15601 gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc aatatcggtc
    15661 gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag gagtttgcct
    15721 tgcggggcgt ggctggcgag tgccggacca tatttcagca ggcgttcaag ttgtgcgtgc
    15781 tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc gtaaatattg
    15841 tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg gaatgccgac
    15901 agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa gctttcggtg
    15961 tacccaaggg gggggggaaa cgggcgtcat tataaagaat ctgatgcgct gacgaaaccg
    16021 tttgcgtgga aataaaatca ccatcgtgaa ttagcaacgc gtgccgccaa tggctgtaat
    16081 aagttgccat ctggcgcagg tttacgcaaa atgccgctca tttatgagta aacctttcac
    16141 tattattacg ttttttcaag ctgggacgcg cacgacacag agaattaact aattgaaaaa
    16201 attaaagatt aattatctgt tcatcggcat tctggcactg ctgctcgcgg tcgctctctg
    16261 gccatccatt ccctggtttg gtaaagccga caaccgtatc gccgccattc aagcgcgggg
    16321 agagttgcgt gtgagcacca ttcatactcc cctgacttat aacgaaatca acgggaaacc
    16381 ttttggcctg gattacgaac tggcgaaaca gtttgccgat tacctcggcg taaaactgaa
    16441 agtgaccgtg cggcagaata tcagccagct gtttgacgac cttgataatg gtaacgccga
    16501 cctgctggcg gcaggactgg tctataacag tgagcgggta aaaaattatc agcctggccc
    16561 tacctattat tccgtgtcac aacaactggt ttataaagtg ggtcagtatc gcccacgtac
    16621 gctgtgcaac ctgacggcgg agcaactcac cgttgcaccg ggtcatgtgg tggttaacga
    16681 tctccagacc ctgaaagaaa caaaattccc ggaattaagc tggaaggtag acgacaaaaa
    16741 aggctctgcg gaattaatgg aagatgtcat cgaaggaaaa ctcgattaca ccattgctga
    16801 ttctgtcgcc atcagcctgt ttcagcgcgt tcacccggag ctcgccgtag cgctcgatat
    16861 caccgatgaa caaccggtga cttggtttag cccgttagat ggcgataata ccctttccgc
    16921 cgccctgctc gacttcttca acgaaatgaa tgaagacggt acgctggcac gcattgaaga
    16981 gaaatacctg gggcatggcg atgattttga ttacgtcgat acgcgcacat ttttacgcgc
    17041 cgtcgatgcg gtactgccgc agttaaagcc cctgtttgag aaatacgccg aagaaattga
    17101 ctggcgtttg ctggccgcta ttgcttatca ggaatcgcac tgggatgcac aggccacttc
    17161 accgacgggt gtgcgcggca tgatgatgtt aaccaaaaat accgcgcaaa gcctcggcat
    17221 tacggatcgt accgatgccg aacagagcat cagcggtggc gtgcgttatt tgcaggatat
    17281 gatgagtaaa gtgccggaaa gtgtgccgga gaacgagcgg atctggtttg ccctcgctgc
    17341 gtacaatatg ggctatgcgc atatgctgga tgcccgcgcc ctgacggcaa aaaccaaagg
    17401 gaatcctgac agttgggctg acgtaaaaca gcgtctgcct ttacttagcc agaaacccta
    17461 ttacagcaag ctgacttacg gctatgctcg tggacatgaa gcctacgctt atgtcgaaaa
    17521 tattcgtaag tatcaaatta gcctggtggg ttatctgcaa gagaaagaga agcaggctac
    17581 agaagcggcg atgcaactgg cgcaggatta tccggcggta tcgcctacgg agttgggcaa
    17641 agagaaattt ccttttctct cgtttctttc ccagtcgtca tcaaactatt tgacccattc
    17701 tccctctctg ctgttttcca ggaaagggag tgaagagaaa caaaattaat ccgtcgagga
    17761 ttgcgctttt ttctgcgctt taatttcctg gcggcgcatg cgaaagaagt cactgagcaa
    17821 cgccgcgcac tcatccgcca gtattccttc cgtaatttcc actcggtgat tcatacccgg
    17881 atgatgcagc acatccatta aagatcccgc agcgccagtt ttcgcgtcac gcgcaccaaa
    17941 gaccacgcga ccaatgcgac tgtggatcat cgctccggca cacattacac atggttcaag
    18001 cgtgacatac aacgtggcgt cgatcagacg ataattttgc atcaccagac caccctgccg
    18061 cagggccatg atttctgcat gtgcggtggg atcatggcga ccaatcgggc ggttccaacc
    18121 ttcgccgatt acccgattgt tatgcactaa taccgcgccg accggcactt cccgctcatc
    18181 ccaggcacgt ttcgccagcg tcagcgcgtg acgcatccag tattcgtggc taaattcgac
    18241 ttcagacaaa aagaaaactc cggttataaa agcgcggcgc attatacacg gacgctatgc
    18301 tttactctat tccagttgct ggagttcacc gcgcggggtt actcgccaac gatgctgaca
    18361 gaaataaagc agcggattgt cctgattact gtcgctatag ccactgtaca gccgcagcgg
    18421 agtgccgatt ttgcgctcca gttgtgcgac cttttcatgt cccagacaac gcatcgtcaa
    18481 tacccaacca ccatagccac gctgaatttg gctggcgata agattaaccc gcggcagcca
    18541 gggcttatcg aaataaaccg cttcaaccag cggctgcgga gagccggtaa tcaaccagat
    18601 atcagcatcg gaacttaaca ggtaggtggt taatcgctcc tgaaccagcg gaaaggcggt
    18661 aacattgtcg cgaaaccagc gcacgaaatc ggcctgcaac gtctgtaaac gtgcttcgct
    18721 gtgaccaaaa gtgcaccccc acagaagcag actcatcggc cagcgtgccg cacgaccttt
    18781 tatcaataac gcaatggcta taatcggtaa caacggcagg acaagtaacg cattcagcgg
    18841 ttggcgacgt agtaaatagc gcagaaaact gccgaacata tcctgctgat gcaatgttcc
    18901 atctaagtca aaaaacacca cacgacgctc gtgagttgcc aaaccatact cctctggact
    18961 gaaacatcct gattaatttt cgctactaca tagcctaaca gatagatcat cacttttccg
    19021 gcaacaatcg accgcatcat ggctaactgg aatttttaat tcatggcaat tagcggcaat
    19081 ggaatataaa attcactcgc gtgtgtctca tattgcctgt tgtcgccact atttcatcgc
    19141 gaggaaagat atgaacggct tacttcgtat ccgtcagcgt taccaggggc ttgcccagag
    19201 tgataaaaaa ctggcggatt atctgctgct acaacctgat acggcgcgcc atttaagctc
    19261 tcagcaactg gccaacgaag ccggagtcag tcagtccagc gtcgtgaagt tcgcgcaaaa
    19321 actcggctat aaaggttttc cggcgcttaa gttggcgttg agtgaagcgc tggcaagcca
    19381 gccggaatcg ccctccgtgc ccattcataa ccaaatccgc ggtgatgatc cgttacggct
    19441 ggtcggcgaa aaactgatta aagaaaatac tgccgctatg tacgcaacgc taaacgttaa
    19501 tagtgaagag aaactgcatg aatgcgtaac aatgttgcgc tctgcgcggc ggataattct
    19561 gaccggtatt ggcgcttcgg gtctggtggc gcaaaacttt gcctggaagc tgatgaagat
    19621 tggcttcaat gctgccgcag tgcgcgatat gcatgcgcta ctcgcaacag tacaggcgtc
    19681 gtcccctgac gatctgttat tagccatttc ctacaccggt gtacgacgcg agttaaacct
    19741 ggcggcagat gagatgctgc gagtgggcgg aaaagtgctg gcgattaccg gctttactcc
    19801 gaatgccctg caacagcgtg cttctcattg cctgtatacc attgccgaag aacaagcgac
    19861 aaacagtgct tcaatctctg cttgtcacgc tcagggaatg ttaacggatt tgctgttcat
    19921 tgcgctgatt cagcaggatc tggaactggc accagaacgt attcgtcata gtgaagcgct
    19981 ggtgaaaaaa ctggtctgag taaagaatgc gcgtataatg cccgcccggt ttgtgttgtt
    20041 ttgagagttt ccttatggcg ttgttaatca ctaaaaaatg catcaattgt gatatgtgtg
    20101 aacccgaatg cccgaatgag gcgatttcaa tgggagatca tatctacgag attaacagcg
    20161 ataagtgtac cgaatgcgta gggcactacg agacaccaac ctgccagaag gtgtgcccga
    20221 tccccaatac tattgtgaaa gatccggcgc atgtcgagac agaagaacag ttgtgggata
    20281 aatttgtgct gatgcaccac gcggataaaa tttaatattc tactctggaa gtagagtatt
    20341 aattatatta ctgggaagcc ttaacgccat tatatttatt taattgatga cattagcata
    20401 atcattcact aagttaattt atatagtatc tgccaagaca cttatttata gttattaaag
    20461 gcgcgtccga ttggttcacc ggacgcacct taagtacgtt tccttgtgtt ataagaacag
    20521 aaggatcagc tgtaaaacag caatgatgat tttgatgacc cgtttaatca ggtatcggca
    20581 atcagtcatt cgtttttcct taaacaagga atgcagccgt tcagattagc ccttacatct
    20641 ccccaaaact gaacgtgcga gttattgagg gtgcatgctg cactccacac cagagctttg
    20701 acgacaccac tcgtttcaat gggggaattc tgtggcatgg tgtaaagcac agcaaaatct
    20761 tcaataacga agccaatttt aatgtactta cgaattggcg gtcacgttaa tctttccatc
    20821 aatattgctt tcttcgtaaa ggctcgagtt tttatgctaa agattgcaag ttgcttgtaa
    20881 aagataagta cactgatcca taatcgctgt tgttgagggt gcatgctgca caaaattaaa
    20941 gttaaaaagt aaaacccccg ttccttacca gttcgggggt tttacttttt aaagagaacg
    21001 gtattatttt aactttcaat aattaccgtg gcacaagcat agtggcgttc atctgccagc
    21061 gttacatgca tatttacaac gccgagcttt tccgccagtt ttaatgcctc accccatagc
    21121 cgtagccgtg gtttgccgag ctcatcattg aatacttcaa attgattaaa cgccagacca
    21181 ttgcggatcc cggtgccaaa cgcttttgct gcggcttctt tcacagcaaa acgcttcgcc
    21241 agaaaacgca ccggctggtg gtgcgttttc cagattgccc attcgttatc gcttaatacg
    21301 cggcgtgcta ggcgctcacc ggatcgggcg atcactgctt ctatgcgagc gatctctacg
    21361 atatccgtgc ctaaacctaa tattgccatt agccacgcgc ttccagcatc agacgcttca
    21421 tttctgccac cgcatctttc agtccggtca tcactgcacg gccaataatg gcatgaccga
    21481 tattcagttc atgcatctca gggatggcgg caatggcttt cacgttgtga taggtcagac
    21541 cgtgtccggc gttaactttc agaccgagac tagcggcaaa ggtcgcggct ttggcgatac
    21601 gcaccagctc ttgcgcctgt tcggcgtcag ttttggcatc agcatagcaa ccggtgtgga
    21661 tctcgataaa cggtgcgcca acctctgccg cagctttgat ctgctcttca tcggcgtcga
    21721 taaagagaga aacctgaatc ccggcatctg tcagacgttt gcaggcatcg cgcatttttt
    21781 cacgctgccc tgcaacgtcc aggccgcctt cggttgttac ttcctgacgc ttttccggta
    21841 ccaggcagca aaaatgtggc ttcgtctcaa cggcgatcgc cagcatctct tcggtcactg
    21901 ccatctccag attcattcgg gtatccagcg tctgccgcag gatgcgcaca tcgcggtcag
    21961 taatgtggcg gcgatcttca cgtaaatgca cggtaatgcc gtccgctccc gcctgctcgg
    22021 caataaacgc cgcctgcacc ggatccgggt aagcagtacc gcgtgcgtta cgcagcgttg
    22081 caatatggtc aatgttgacg cctaacagta attcagccat gacaatcctc atcattcata
    22141 atgtgttttc accgttcgct taggcataaa ctgccggaac agttccctgc tctttaatgg
    22201 tttaccgcca agatacggct taagcgccat gcgggtaaag cgtttcgcgg cgcgtagtgt
    22261 gtctgcgtca ggaaattccc gcgcgtttaa cgcttttaac tgccttccgg tgaacgtttt
    22321 attgtcgata acgacgcttg cgataaaccc tttttcttcg cgataacgat acgtcatggt
    22381 gtcatctacc ggctcgccgc tacccgcgca ataggtaaaa ttgacgccat agcccagatg
    22441 cccgagcaat gccagttcaa agcggcgcag cgcgggttct ggcgtaccag tggcacctgc
    22501 aagagactga atgcagtgca aataatcgaa aaagagttca gagaagcgcg tctcgtattc
    22561 cagtacgcgg gagagaagtt cgttgatgta cagaccgctg taaagcgtga taccgcttaa
    22621 tggcagcgcc agcgagacag cttcagcact gcgcagcgtt ttgacttcgc cacgcccgcc
    22681 gaaacgtagc aagagagggg tgaaaggctg taatgcacct ttcagagtag agcgtttaga
    22741 gcgtgcgcct ttggcaacca gacgcacgcg acccgattcc tccgtgaaga cgtccagcat
    22801 caggctggtt tcgctccacg ggcgactatg caggacaaat gcgcgctgcc agccttccat
    22861 cggagtgact cttaaagatc gtcaacgtaa ccgagactgc gcagtgcgcg ttcgtcgtcg
    22921 gcccaaccgg atttcacttt tacccacagt tcaaggtgga caggcgcttc gaacatttcc
    22981 tgcatgtctt tacgcgcttc aatcccgatg gttttgatct tggccccttt gttgccaata
    23041 accatcttct tctgcccttc acgctcaacg agaatcaaac cattgatgtc ataaccaccg
    23101 cgttcgttag agacgaaacg ttcgatctcc acggtcacgg agtacggcag ttcagcgccg
    23161 aggaaacgca tcagtttttc gcggatgatt tcagacgcca taaaacgctg tgagcgatcg
    23221 gtgatgtaat cttccgggaa gtgatgggtc gcttcaggca gatgcttacg cacgattgct
    23281 gcgatagtgt cgacattcag cccggtttca gcagagatcg gcacgatatc gaggaagttc
    23341 atctgacttg ccaggaactg taagtgcggc agcagatcgg ctttttcctg cacgttatcc
    23401 actttgttca ccgcgaggat caccggcgct ttgccatcgc gcagtttgtt gagcaccatt
    23461 tcgtcgtccg gcgtccagcg ggtgccttca acgacaaaaa tcaccagctc aacgtcgcca
    23521 atagagctgc tcgccgcttt gttcatcagg cggttaatag cgcgtttttc ttccatatgc
    23581 aggccagggg tatcaacgta gatcgcctga tacgcgcctt cagtatggat ccccacaatg
    23641 cggtgacgag ttgtttgcgc tttgcgggag gtgatggaga tcttctgccc cagcagtttg
    23701 ttcaacaatg tggatttgcc aacgttcgga cgtccgacga tggcaataaa tccgcagtaa
    23761 cttttatcga tgctcattcc agctccagtt ttttcaacgc ctgttcggcg gcagcctgct
    23821 cagccttacg acggcttgaa cctgtgccaa ccaccggttc actcaggccg ctgacctggc
    23881 agtggatagt aaattcctga tcgtgcgctt cgccacgtac ctggactacc agataagtcg
    23941 gcagcggcag atggcgaccc tgcaaatatt cttgcaagcg cgttttcgga tctttttgtt
    24001 tatcgcctgg gctaatttcg tccaaacgag tttgatacca gttgaggatt aatttctcga
    24061 cggtttgaat atcactgtcg aggaatacgc caccaattaa tgcttcgacg gtatcggcga
    24121 gaattgactc acgacgaaat ccaccgcttt taagttcacc aggccctaaa cgtaagcact
    24181 cgcctaactc aaattcgcgc gccagttccg ccagcgtatt gccacggacc agcgtggcgc
    24241 gcatccggct catatcgcct tcatccacac gagggaaacg gtgataaagc gcattggcga
    24301 taacgtagct cagaatagag tcgcctaaaa attctaaacg ctcgttatgt ttgctgctgg
    24361 cactacgatg agttaatgcc tgctgcaaca gttcctgatg attaaaagtg tagcccagct
    24421 tccgttgaag ccgattaatt acgatggggt tcatgcgata ccaataaata aatgcgtcaa
    24481 caattcagca cacgaaacag acctgatata catggctctg ctaactgctt cgctgcagtt
    24541 tcagtttacc ggtatatggg gaccaacgct gtttcgtgtg ccgtggcaac ctggaggtgc
    24601 caaccttaaa cttcggggga atattctata cacaacgacg ggggatgtcg ttagccacgg
    24661 gagatttatc tcataaataa ttcacgttgt cgccataacg gcgacaacgt gaacgaagat
    24721 gggctattaa tggatgccgc caatgcgact taagcgcaca cctgtcggcc attcgccttc
    24781 ttgcttatcg aaactcatcc agatagccgt tgcccgaccg accagattcg cttcaggcac
    24841 aaaaccccag taacggctgt ccgcgctgtt gtcgcggttg tcgcccatca tgaagtattg
    24901 tcccggcgga acaatccagg ttgccagttg ttgccctggc tgctggtaat acatccccac
    24961 ctggtcctgc gcaatcggca ctgtcagaat tcggtgcgtc acatcaccca gtgtctcttt
    25021 acgctcggaa agacgaattc cattttcttt ggtttcgttt ttcggcactt caaagaatcc
    25081 gctggtcgct tccccaccat tacggcgtga gaaggtctga acgaaatcgc tcggttccac
    25141 gttggagtag gtgaccggta gcgcgttttc acacgcctgg ccggaactgc atcccggttg
    25201 aatcgtcagc tcttttgaga ccggatcgta agtgacttta tcgcccggta aacccaccgc
    25261 gcgcttgatg taatcaagct ttggatcttc cggatattta aagaccacga tatcgccgcg
    25321 tttcggatga ccggtttcga tcagcgtttt ctggtagata ggatctttaa tgccataagc
    25381 aaacttctct accagaataa aatcaccgat taacagagtc ggcatcatcg aacctgacgg
    25441 gatctggaac ggttcataaa taaacgaacg tacaatcaat acgatagcca gcaccggaaa
    25501 aacagaagca ccggtttcca gccagccagg cttcggcgca acctttttca gggttgcttt
    25561 atccagtgag tcaccggcag ccgcctgcgc cgctgcctga cgttcccgcc gtttaggtgc
    25621 gaaaaagaat ttatccacgc accataaaat gcccgtcacc agtgtggcaa tcaccagaat
    25681 cagggcaaac atattcgcca tgccaactcc taagggttat ttgttgtctt tgccgacgtg
    25741 cagaatggcg aggaacgctt cctgcggcag ctcgacgtta ccgatctgct tcatgcgttt
    25801 cttaccttct ttctgcttct gcagcagctt tttcttacgg ctgatatcgc cgccataaca
    25861 tttagccagt acgtttttac gcagctgttt cacggtggat cgcgcaataa tgtgcgtacc
    25921 aatcgctgcc tgaatggcga tatcaaactg ctggcgtggg atcagatctt tcatcttctc
    25981 caccaactcg cgaccgcggt tttgcgaatt atcacggtgg gtgatcagcg ccaacgcatc
    26041 aacacgttca ccgttgatta atacgtctac acgtaccatg tcggacgcct ggaagcgttt
    26101 gaagttgtaa tccagagacg cataaccacg cgaggtagat ttcaggcgat cgaagaagtc
    26161 gagcaccact tccgccatcg ggatctcgta cgtcagcgcc acctgattac cgtggtaaac
    26221 catattggtc tgcacgccgc gtttttctac gcacagcgta ataacgttgc cgagatatgc
    26281 ctgcggcagc agcatgtgac actctgcaat cggctcgcgc agttcgtaaa tgttatttac
    26341 cgcaggcagc ttggatgggc tgtcgacgta gataacttcc cttgacgtgg tttcaacttc
    26401 atacactacg gtcggcgcag tggtgatcag atccagatcg tattcacgtt ccagacgttc
    26461 ctggatgatc tccatgtgca gcaggccaag gaagccgcag cggaaaccaa agcccagcgc
    26521 gctggagctt tccggctcat agaacagtga ggcatcattc aggctgagtt tacccagcgc
    26581 gtcacggaag gcttcatagt cgtcggaact taccgggaac agaccggcgt atacctgcgg
    26641 tttgactttc ttaaagccag gcagcgcctt ttctgccgga ttacgcgcca gcgttaaggt
    26701 atcgccgact ggagcgccgt ggatatcttt aatcgcacat accagccagc ctacttcgcc
    26761 acatttcagt tcagtgcggt caacctgttt cggcgtgaag atgcccagac ggtcggcgtt
    26821 ataggtctgc ccggtactca tgactttcac tttgtcgccc ttacgcaggg tgccgttttt
    26881 aatacggata agtgaaacaa cgcccaggta gttgtcgaac catgagtcga taattagtgc
    26941 ctgcaacggg ccttccggat cgccttccgg cggcggaatg tcgcgcacca gacgttcgag
    27001 aacgtcctgc acgccaacgc cggttttcgc tgaacagcgc accgcgtcag tggcgtcgat
    27061 gccgacgata tcttcaattt cttccgccac gcgttcagga tcggctgccg gcaggtcaat
    27121 cttgttcagt actggcacca cttcgagatc catttccatg gcggtgtagc agtttgccag
    27181 ggtttgcgct tccacgccct gcccggcgtc gaccaccagc aatgcacctt cacaggcagc
    27241 cagcgaacgg gaaacttcat aggagaagtc tacgtggccc ggggtgtcga taaagttaag
    27301 ctgataggtt tcgccgtcag acgctttgta gtccagcgtc acgctttgcg ctttgatggt
    27361 aatgccacgc tcacgctcaa gatccatgga atcgagaacc tgcgcctcca tttcacggtc
    27421 agacaggcca ccgcagatct ggataatacg gtcagacagc gtcgatttac cgtggtcaat
    27481 gtgagctatg atcgaaaagt tacgtatatt cttcataaag tatgattatt gtgccttaat
    27541 gcccggttaa ccaggctttt agaagtcgct gttctgagct taacgtctgt attaatagaa
    27601 acgccgcatt ctacactaca acattgaggc gaggaaatgt tcataccgta tggattgtgg
    27661 tatctggaaa cgtcctcgca tttgttatgc aaaatgcaac aaagccagtg aaatcactgg
    27721 ctcgcgtctt ccgaagatgt ttcaaatcgc acaaggccag gcggcaatgc cacgcttaag
    27781 atgatcggtt gccattctgc ccgggcagca aacttacgcg agtagccgcg cgcaatcagg
    27841 aacccgccaa taccgccgag gaccgcacca cataatgctg cgacgtcaga agcaaagagt
    27901 agctgaaata gcgaagcgat gaggaataat cccaccagcg gcgacatata aaccagtaat
    27961 gcggagctaa gcaggctgcc ttcggcgatc cccaattcca ctttttgccc cggcactaac
    28021 ggttcatcac agggtacgac aatggtatgc gtggtttgcg ggccaagttt attcaacacg
    28081 cggctaccgc aaccggcgcg tgaagcgcag ctgctgcatg aagctttaac atcacaactg
    28141 accagcgcct gcccgttttg ccaggagacg acggtagccc actctttgat cattgcgctg
    28201 ccccgaactt aatattctcg gcaatgcgtt tcgccgtttg cggcggcagt tcaccgacaa
    28261 tggtgatttc ggcgttatca cgtacgcttg tactgacggt tctgcgtccg gtgcgcaaca
    28321 tctgatcggt gctcgatggc gtagcgcggt taacgtttac cgagaagctg aataatccgt
    28381 cagaatagag acgtgattcg ataggcatgt tgtccatcgt cggtagcgga cgtcgactac
    28441 tggaaacttc gctaaaaccc tgtggcaacc aggttggcgt ccagctgaat ttagcttttt
    28501 cacctacagg aacagaaagc aacggcggca aatttgcctt cgccagcgtc tgcatactgc
    28561 tgctgatatc ctgattgacg ttaaaagcaa tcacgcgaaa ttgttccagc gtttcaccat
    28621 cgcgatcaag gagatcaacc cgcatcggta atttcgattc ggtgtccatc cacacgatgt
    28681 agctgtagcg tgtaccatct cgggcaacca cgcgaatgac ttcgcaaaga cgatcagcaa
    28741 tacgcgtgcg cccgacggag ataaagtcgt agtaaggaga aaggcgtttg aaatcggtat
    28801 agatgagcga tggcagagaa tcaacaatgt aatcgccatt aagcgtgaac ggttcaagtc
    28861 ccggttcaaa atagctgatt tcatcgccgc gctgtaccac ttcccggcgc gggccatcca
    28921 tttgcaacaa ttgtgcaaga ggacggttat cgaggcgtgc atgtcgataa cgcagagact
    28981 caacaccctg tttattgatg ctgatgaatg acagctcgta attcagtgac tgactggcca
    29041 ggttcatctg ctgtaataac gccccggacg cgggagtggc cgaggcgtta gcagagaata
    29101 acaggctacc tgtcactaat gacatggcaa accaaagttg cttcattact gcgattgcgt
    29161 tcctaaagtt tgaattcctg gcacctgtac agcggcttgc tgggtttgcg cctgctcaaa
    29221 ctgaagctgt tcagagtgga gtcggcgttg cagttcgtaa tcctgcaaca ttgcattaat
    29281 gcgacgacgc tgctcctgta cctgctgctg ttgaccattg tttgcggtcg cttcagaagg
    29341 tactcccagg cttaccgggc tggctttacc catcatcggc agtgtattaa ataccggcgt
    29401 ttcgggctgc tgggacgttt cagattgtcc attatagtgc tggacgccaa cgataactgc
    29461 aagcgatacg catgctgcta cgcccatttg ggtaagctgt gccgcccacg gacgtacttt
    29521 ctgccagaat ggcattttct gccattgatg cggcgcaggc tgggcttccg ggatcaatgt
    29581 cgccggttga cgtactggct cttcttcaat ggcggccatc acgcgtgaag agatatcgaa
    29641 atggagcacc tcgggagtat caccccgcat tgagtcacgg attaagtgat agctttccca
    29701 ggttttctgc atttctgggt tatgagccag ttcgttaagc agctcactat ccagcgtttc
    29761 gccatccatt aaagcggaaa gttgttcttt ctgcatgcct aataccctta tccagtatcc
    29821 cgctatcgtc aacgcctgat aagcggttga actttgttat caatagcttc cctcgctcgg
    29881 aagatacgtg aacgcaccgt acctaccgga caatccatga tagcggctat ctcttcatag
    29941 ctcaggccat ccagctcccg caaggttatt gccatgcgca aatcttccgg gagggactca
    30001 atagttcgga aaactatctg tctcagttct tctgacaaca ttaagttctc agggttcgaa
    30061 atttctttca acgcgccgcc actttcgaag ttttcagctt caatggcatc cacatcactg
    30121 gaaggtggac gacgcccctg agcaaccagg taatttttcg ctgtatttac agcaatccga
    30181 tacagccatg tataaaaagc gctatctccc cggaacgaat ccagcgcacg ataggcttta
    30241 ataaaagctt cttgtaccac atcgggaaca tcacccgacg gcacatagcg ggaaaccaga
    30301 ctcgccactt tatgctgata gcgcactacc agtaagttaa aggctttctg atctcccttc
    30361 tggacccgtt caaccaggac ctggtccgtt aactgctcgc tcatccgagg taaagtctcc
    30421 ccaaaccaaa tttccacgcg ctatcgaaac gccactccat tagctgcaat ttgagcaagc
    30481 aaagggttag agtgtctcgt ttttgtaaag ttccgtaacg catctgtttt tgtttgtcat
    30541 gctgtagacg gatcattatc tatcattata agtctacaga atctgaacat cgcattatct
    30601 gtgtagaaat gcccatttaa ctgcctgaag agtaacccaa cggccttttt atttcaccac
    30661 ctaatcctcc accagccagt aacttctctt tttctcgccg ccctgcatca gcgtgtttag
    30721 caactgtaac aaatattaaa atagcaggtg tttattcgca caacatgatg ctatactgac
    30781 caaaccgtgt ttagtaaatt aaacaaagaa aatgaatact ctccctgaac attcatgtga
    30841 cgtgttgatt attggtagcg gcgcagccgg actttcactg gcgctacgcc tggctgacca
    30901 gcatcaggtc atcgttctaa gtaaaggccc ggtaacggaa ggttcaacat tttatgccca
    30961 gggcggtatt gccgccgtgt ttgatgaaac tgacagcatt gactcgcatg tggaagacac
    31021 attgattgcc ggggctggta tttgcgatcg ccatgcagtt gaatttgtcg ccagcaatgc
    31081 tcgttcctgt gtgcaatggt taattgacca gggggttttg tttgataccc acgttcaacc
    31141 gaatggcgaa gaaagctacc atctgacccg tgaaggagga catagtcacc gtcgtattct
    31201 tcatgccgcc gacgccaccg gtagagaagt acaatccacg ctggtgagca aggcgcagaa
    31261 ccatccgaat attcgtgtgc tggagcgcag caacgcagtt gatctgattg tctctgacaa
    31321 aattggcctg ccgggcac
//
LOCUS       DAJVOY010000029        30944 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVOY010000029 DAJVOY010000000
VERSION     DAJVOY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.029"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OQL90_001174"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OQL90_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1368026.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OQL90_001175"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OQL90_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1368027.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1338..1766
                     /gene="rplK"
                     /locus_tag="OQL90_001176"
     CDS             1338..1766
                     /gene="rplK"
                     /locus_tag="OQL90_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1368028.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1770..2474
                     /gene="rplA"
                     /locus_tag="OQL90_001177"
     CDS             1770..2474
                     /gene="rplA"
                     /locus_tag="OQL90_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1368029.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2887..3384
                     /gene="rplJ"
                     /locus_tag="OQL90_001178"
     CDS             2887..3384
                     /gene="rplJ"
                     /locus_tag="OQL90_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1368030.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3451..3816
                     /gene="rplL"
                     /locus_tag="OQL90_001179"
     CDS             3451..3816
                     /gene="rplL"
                     /locus_tag="OQL90_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1368031.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4136..8164
                     /gene="rpoB"
                     /locus_tag="OQL90_001180"
     CDS             4136..8164
                     /gene="rpoB"
                     /locus_tag="OQL90_001180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1368032.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8241..12464
                     /gene="rpoC"
                     /locus_tag="OQL90_001181"
     CDS             8241..12464
                     /gene="rpoC"
                     /locus_tag="OQL90_001181"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1368033.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12677..13216
                     /locus_tag="OQL90_001182"
     CDS             12677..13216
                     /locus_tag="OQL90_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCS1368034.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OQL90_001183"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OQL90_001183"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1368035.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OQL90_001184"
     CDS             complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OQL90_001184"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1368036.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OQL90_001185"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OQL90_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1368037.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OQL90_001186"
                     /pseudo
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OQL90_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OQL90_001187"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OQL90_001187"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1368038.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OQL90_001188"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OQL90_001188"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1368039.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OQL90_001189"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OQL90_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1368040.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="OQL90_001190"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="OQL90_001190"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1368041.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="OQL90_001191"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="OQL90_001191"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1368042.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21682..22353
                     /gene="nfi"
                     /locus_tag="OQL90_001192"
     CDS             21682..22353
                     /gene="nfi"
                     /locus_tag="OQL90_001192"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1368043.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22396..22986
                     /locus_tag="OQL90_001193"
     CDS             22396..22986
                     /locus_tag="OQL90_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1368044.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23173..23445
                     /gene="hupA"
                     /locus_tag="OQL90_001194"
     CDS             23173..23445
                     /gene="hupA"
                     /locus_tag="OQL90_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1368045.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23458..24153
                     /locus_tag="OQL90_001195"
     CDS             23458..24153
                     /locus_tag="OQL90_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1368046.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OQL90_001196"
     CDS             complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OQL90_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1368047.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24818..26194
                     /gene="zraS"
                     /locus_tag="OQL90_001197"
     CDS             24818..26194
                     /gene="zraS"
                     /locus_tag="OQL90_001197"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1368048.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26191..27515
                     /gene="zraR"
                     /locus_tag="OQL90_001198"
                     /pseudo
     CDS             26191..27515
                     /gene="zraR"
                     /locus_tag="OQL90_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OQL90_001199"
     CDS             complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OQL90_001199"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1368049.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OQL90_001200"
     CDS             complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OQL90_001200"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1368050.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7337 a         7987 c         8433 g         7187 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgactcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcttggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgagtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt atttttatgg
     1261 gccttgcccg taaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1321 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1381 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1441 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1501 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1561 cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc tggtgccggt aagccgaaca
     1621 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1681 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1741 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1801 tccgcgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1861 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1921 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     1981 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2041 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2101 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2161 tgggtccgcg cggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2221 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2281 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2341 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2401 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2461 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2521 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2581 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2641 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2701 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2761 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2821 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2881 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     2941 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3001 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3061 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3121 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3181 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3241 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3301 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3361 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3421 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3481 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3541 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3601 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3661 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3721 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3781 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3841 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3901 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     3961 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4021 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4081 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4141 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4201 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4261 tcctgaaggg cagtatggtc tggaagcagc tttccgttcc gtattcccga ttcagagcta
     4321 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4381 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4441 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4501 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4561 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4621 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4681 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4741 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4801 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4861 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4921 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     4981 caaacttatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5041 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5101 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5161 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5221 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5281 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5341 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta tcctgagcaa
     5401 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5461 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5521 ccagttccgc gttggcttgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5581 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5641 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5701 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5761 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5821 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5881 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     5941 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6001 cctggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca aaggcgaatc
     6061 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6121 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6181 tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6241 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6301 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6361 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6421 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6481 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6541 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6601 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6661 caccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6721 ctccaaacta gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6781 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6841 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6901 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     6961 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7021 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7081 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7141 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7201 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7261 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7321 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7381 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7441 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7501 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7561 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7621 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7681 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7741 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7801 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7861 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7921 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     7981 cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga acggtcgtac
     8041 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8101 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8161 gtaattctcg ctcaaacagg tcactgctgt cgggttaaaa cccggcagcg gattgtgcta
     8221 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8281 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8341 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8401 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8461 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8521 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8581 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8641 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8701 tggaacgtca gcagatcctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8761 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8821 tggagcaaga gtgcgaacag ctgcgcgaag agctgaacga aaccaactcc gaaaccaagc
     8881 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     8941 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9001 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9061 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9121 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9181 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9241 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9301 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9361 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9421 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9481 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9541 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9601 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9661 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9721 cgaacggcga accaatcatc gttccgtctc aggacgttgt tctgggtctg tactatatga
     9781 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9841 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9901 agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
     9961 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10021 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10081 tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc
    10141 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10201 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10261 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10321 ccaaagcgat gatggataac ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag
    10381 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10441 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg gatggctcca
    10501 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10561 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcaaactccg
    10621 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10681 gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgat gttaaagagc
    10741 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10801 ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10861 agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10921 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aagggtgaag
    10981 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11041 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11101 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11161 ttatcacttc ccgtaatact gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11221 gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggcg
    11281 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11341 ttgtacgctt tactgacatg atcgacggtc agaccattac tcgtcagaca gacgaattga
    11401 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11461 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatt ccgggtaccg
    11521 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11581 agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt accaaggaca
    11641 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg
    11701 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11761 gtcttgttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11821 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11881 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg
    11941 ttaacgaagt acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg
    12001 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12061 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12121 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12181 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12241 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12301 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12361 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12421 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12481 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
    12541 tcagaatatt taaggaattt ctgaatactc ataatcaatg tagagatgac taatatcctg
    12601 aaactgactg aactaattga gtcaaactcg gcaaggattc gatactattc ctgtgtaact
    12661 ttcttaagga acgagaatga aacaggaagt ggaaaagtgg cgaccttttg gacatccgga
    12721 tggtgatatt cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12781 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12841 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12901 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12961 tattttggcg ctgccagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    13021 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13081 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttat cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctt cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttgccgttg
    14701 atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgctgca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggtg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga gaatttgtcg
    16261 ttacagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatca tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgat gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggt gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcaac agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    18301 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcgatct gcggatcgcc atatgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcaaact gcggctgttc tttgctgccg ccgattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgaacgcc agagtgtgtg ccagtgaggt aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctttt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgtgctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagt cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataag
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggtgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc gatggcagaa accggttgtg atgcgttggg cctcgactgg
    21421 acaacggata tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacaa
    21661 tatcaccgct aaggagtgat tatggatctc gcgtcattac gtgctcaaca aatcgaactg
    21721 gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct gatcgccgga
    21781 gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa
    21841 tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac
    21901 attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg
    21961 tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt
    22021 ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt ggcgaaaaaa
    22081 cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg
    22141 gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt
    22201 atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg
    22261 aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg
    22321 gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt
    22381 atttgagaac ttaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc
    22441 tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg
    22501 ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc
    22561 tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt
    22621 gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat
    22681 gcctgcgtgg tgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac
    22741 gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggtt
    22801 ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac
    22861 attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata
    22921 aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag
    22981 caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacctggagt cttccctttc
    23041 gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca
    23101 ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg
    23161 taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg
    23221 tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg
    23281 aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct
    23341 gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta
    23401 ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg
    23461 aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg
    23521 ctggctggcg ctctgcttct cactgcctgt agtcataact cttcacttcc tccctttacc
    23581 gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat
    23641 aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag
    23701 tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg
    23761 gtgaatattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca acgtgaatcc
    23821 gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat
    23881 cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc
    23941 tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta
    24001 gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc
    24061 gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc
    24121 tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac
    24181 cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt
    24241 cagccatcgc gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca
    24301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
    24361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
    24421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
    24481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
    24541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
    24601 tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc
    24661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
    24721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
    24781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
    24841 agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg
    24901 gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
    24961 gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat
    25021 ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc
    25081 tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
    25141 cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac atccgggaga
    25201 tgaagaagcg tggcggcgga tcgatagcgc agacggtgag cctgttctgg aaatttatcg
    25261 ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc agcagtatgc
    25321 cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga
    25381 tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    25441 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    25501 aatgaagcgc aaagagaagc tggtggcact ggggcatctg gcggcaggcg ttgcccacga
    25561 aatccgtaat ccactttcct caattaaagg gctggcgaaa tactttgccg aacgcgcgcc
    25621 agcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaagccg accgtttaaa
    25681 ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt
    25741 ggatctcaac acgctgatta accactcatt acagctggta agccaggatg caaacagccg
    25801 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga
    25861 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttgttcagca
    25921 tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    25981 cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac
    26041 caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    26101 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    26161 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    26221 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    26281 aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt
    26341 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    26401 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    26461 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    26521 gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa
    26581 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    26641 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cagtgctgat ccacggcgat
    26701 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    26761 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    26821 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    26881 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    26941 gtgcgtctgc tgcgtgcgat tcaggagctc gaagttcagc gtgtcggtag caaccagact
    27001 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    27061 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    27121 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    27181 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    27241 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    27301 ctgctgccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc agtacgccga
    27361 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtggaaaaa gaggtgattc
    27421 tggcggcact ggagaaaacg ggcggcaaca aaactgaagc cgcccgtcag ttagggatca
    27481 cgcgtaaaac gttattggca aaactgtcgc gttagttctg ctcgcgttcg atagcacgcc
    27541 agccgatatc tttccggcag aagcagtcgt cccagtggat atcggtcatt aaggcatagg
    27601 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27661 cgttggtcac tacctgctcg tcatccgcca gttttgtgcc cgcgtggaac actttgccgc
    27721 ctgccacttc ttccagcagc aggccgtgga tcacgtcacc ggtgcggtaa tcacccggat
    27781 atccacccgc agccatcacc acgccgagag aagcgcgttc atcccactcg gacgttttct
    27841 cgtccagttt gctttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27901 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27961 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    28021 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacgtcat
    28081 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc ggtgtttggt ccggtatctt
    28141 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28201 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28261 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28321 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28381 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28441 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28501 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcggcgcgg aaggtatcga
    28561 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28621 gtgcgaaatc caacagcgcc gggatatcgg tcacgccaat agcaacgttt tgcagcgcgg
    28681 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28741 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28801 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28861 ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa ccgccaggct ggattacgca
    28921 ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28981 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    29041 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29101 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcgcg
    29161 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29221 ccccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat caagacccgg
    29281 aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat
    29341 tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact gacgagaaat
    29401 gatggcctgt gcggtttccg catctagctc gcggttaaag gcaatgatgc cgccgaatgc
    29461 ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29521 tacaccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29581 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29641 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29701 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29761 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg tacgccggaa ccatgctgcc
    29821 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29881 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29941 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag cggcggagcg
    30001 caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcagccttc
    30061 acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30121 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30181 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30241 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30301 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30361 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30421 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30481 gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30541 ttctctttca atggtgatca caattttgac agtggctacc gtgggaaaaa tacagaaatt
    30601 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30661 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30721 taatgaacct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30781 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30841 cactcacgac agtgcgctaa agctcgtcgc aactgctctt taacaattta tcagacaatc
    30901 tgtgtgggta ctcgaagata cggattctta acgtcgcaag acga
//
LOCUS       DAJVOY010000030        29256 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVOY010000030 DAJVOY010000000
VERSION     DAJVOY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.030"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..154
                     /locus_tag="OQL90_001201"
     CDS             <1..154
                     /locus_tag="OQL90_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HCS1368051.1"
                     /translation="NYYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTL
                     VKSFDW"
     gene            429..3278
                     /gene="rcsC"
                     /locus_tag="OQL90_001202"
     CDS             429..3278
                     /gene="rcsC"
                     /locus_tag="OQL90_001202"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1368052.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGGEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="OQL90_001203"
     CDS             complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="OQL90_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1368053.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="OQL90_001204"
     CDS             complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="OQL90_001204"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1368054.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7556..8686
                     /gene="ompC"
                     /locus_tag="OQL90_001205"
     CDS             7556..8686
                     /gene="ompC"
                     /locus_tag="OQL90_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1368055.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8798..9853
                     /gene="apbE"
                     /locus_tag="OQL90_001206"
     CDS             8798..9853
                     /gene="apbE"
                     /locus_tag="OQL90_001206"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1368056.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9927..10979
                     /gene="ada"
                     /locus_tag="OQL90_001207"
     CDS             9927..10979
                     /gene="ada"
                     /locus_tag="OQL90_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1368057.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCPVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10979..11629
                     /gene="alkB"
                     /locus_tag="OQL90_001208"
     CDS             10979..11629
                     /gene="alkB"
                     /locus_tag="OQL90_001208"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1368058.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11705..13348
                     /gene="yojI"
                     /locus_tag="OQL90_001209"
     CDS             11705..13348
                     /gene="yojI"
                     /locus_tag="OQL90_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1368059.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13566..15212
                     /gene="mqo"
                     /locus_tag="OQL90_001210"
     CDS             13566..15212
                     /gene="mqo"
                     /locus_tag="OQL90_001210"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCS1368060.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15362..15850)
                     /gene="eco"
                     /locus_tag="OQL90_001211"
     CDS             complement(15362..15850)
                     /gene="eco"
                     /locus_tag="OQL90_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1368061.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15985..16149)
                     /locus_tag="OQL90_001212"
     CDS             complement(15985..16149)
                     /locus_tag="OQL90_001212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368062.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            16258..16752
                     /gene="napF"
                     /locus_tag="OQL90_001213"
     CDS             16258..16752
                     /gene="napF"
                     /locus_tag="OQL90_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1368063.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16742..17005
                     /gene="napD"
                     /locus_tag="OQL90_001214"
     CDS             16742..17005
                     /gene="napD"
                     /locus_tag="OQL90_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1368064.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            17002..19488
                     /gene="napA"
                     /locus_tag="OQL90_001215"
     CDS             17002..19488
                     /gene="napA"
                     /locus_tag="OQL90_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1368065.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            19495..20190
                     /gene="napG"
                     /locus_tag="OQL90_001216"
     CDS             19495..20190
                     /gene="napG"
                     /locus_tag="OQL90_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1368066.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20177..21040
                     /gene="napH"
                     /locus_tag="OQL90_001217"
     CDS             20177..21040
                     /gene="napH"
                     /locus_tag="OQL90_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1368067.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21037..21486
                     /gene="napB"
                     /locus_tag="OQL90_001218"
     CDS             21037..21486
                     /gene="napB"
                     /locus_tag="OQL90_001218"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1368068.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21496..22098
                     /gene="napC"
                     /locus_tag="OQL90_001219"
     CDS             21496..22098
                     /gene="napC"
                     /locus_tag="OQL90_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1368069.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22117..22734
                     /gene="ccmA"
                     /locus_tag="OQL90_001220"
     CDS             22117..22734
                     /gene="ccmA"
                     /locus_tag="OQL90_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1368070.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22731..23393
                     /gene="ccmB"
                     /locus_tag="OQL90_001221"
     CDS             22731..23393
                     /gene="ccmB"
                     /locus_tag="OQL90_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1368071.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23435..24172
                     /gene="ccmC"
                     /locus_tag="OQL90_001222"
     CDS             23435..24172
                     /gene="ccmC"
                     /locus_tag="OQL90_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1368072.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24169..24378
                     /gene="ccmD"
                     /locus_tag="OQL90_001223"
     CDS             24169..24378
                     /gene="ccmD"
                     /locus_tag="OQL90_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1368073.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24375..24854
                     /gene="ccmE"
                     /locus_tag="OQL90_001224"
     CDS             24375..24854
                     /gene="ccmE"
                     /locus_tag="OQL90_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1368074.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24851..26800
                     /gene="ccmF"
                     /locus_tag="OQL90_001225"
     CDS             24851..26800
                     /gene="ccmF"
                     /locus_tag="OQL90_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1368075.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     MLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIAIASLLSGRLDSTYARFTRPWTLAAW
                     IFLTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKA
                     WTLLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAA
                     RGHKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPF
                     FNTMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKV
                     VAMTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQN
                     YSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEK
                     RYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMAL
                     GGLLCLFDPRYRKRVSPQKTAPEAV"
     gene            26797..27354
                     /gene="dsbE"
                     /locus_tag="OQL90_001226"
     CDS             26797..27354
                     /gene="dsbE"
                     /locus_tag="OQL90_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1368076.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27351..28403
                     /gene="ccmH"
                     /locus_tag="OQL90_001227"
     CDS             27351..28403
                     /gene="ccmH"
                     /locus_tag="OQL90_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1368077.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28438..29085)
                     /gene="narP"
                     /locus_tag="OQL90_001228"
     CDS             complement(28438..29085)
                     /gene="narP"
                     /locus_tag="OQL90_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1368078.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6682 a         7219 c         8105 g         7250 t
ORIGIN      
        1 caactactat ggtgggcgca gcgaaacgga attaaccacc gcccaactga cgctgatcac
       61 cgatgaagga tcggtcaatg agaaacagga aacatttatt gtaccgatgc gtaatgctgg
      121 cgagctgacg ctggtgaaaa gttttgactg gtgatccatc ccttcacaca acctggctct
      181 ctcttttttc agataagact gctgggcgat ctgccgacgc caggatataa ccattaacaa
      241 gcgtcggcag attggtcatt tcttattttt cgtactgaga actgtccatc tactccggct
      301 ttcctggtta aggcaatggt ttctaagcga tacgcattat tgcaataatg acagaacatt
      361 gcggtaacac gcttttaccg ctaccttaac cacactccat cggtcacctg aggcggagct
      421 tcgccccttt gaaatacctt gcttcttttc gtacaaccct gaaagcctcg cgctacatgt
      481 tcagagcatt ggcgttagtg ctctggctgt tgattgcttt ttcatccgtt ttttacatcg
      541 ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga atttaatctg agttccgatc
      601 aggctcagcg ctttattcaa cgcacctctg atgtgatgaa agagctgaag tacatcgccg
      661 aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg
      721 atgtgcctgc gtttgaaccg ctgtttgccg actccgattg ttccgcaatg agtaacacct
      781 ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg
      841 cggcttacga tctaaaccgg gtatttttaa tcggcagcga taacctctgc atggccaatt
      901 tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt gaaagctttg catgaacgca
      961 tcaataaata tcgaaatgca ccacaagatg atagcggcag taacctctac tggatcagcg
     1021 aaggtccgcg ccctggcgtc gggtattttt acgcgttgac gccagtttat ctggcgaacc
     1081 ggttgcaggc gcttttgggg ggcgagcaga ccatccggat ggagaacttt ttcttaccgg
     1141 gtacgttgcc gatgggggtt accattcttg atgaaaatgg tcataccctg atttcgctta
     1201 ccggaccaga aagtaaaatt aagggcgagc ctcgctggat gcaggaacgc tcctggtttg
     1261 gctatacgga agggttccgg gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa
     1321 gcatcgtgta ttcggtgccg gttgataagg tgctggaacg cattcgcatg ttgatcctta
     1381 acgcaatttt gctgaatgtg cttgccggag ctgcattgtt tactctcgca cggatgtacg
     1441 agcgacgtat tttcattccg gcggaaagcg acgccctgcg actggaagaa catgagcagt
     1501 tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg cattttgcgt accgctgatg
     1561 gcgtcaatat tttaagtaac gaactggcgc atacctatct caatatgctt acgcatgagg
     1621 accgccaacg actgacgcaa attatctgtg ggcagcaggt caattttgtt gatgtcctga
     1681 ccagcaacaa taccaatctg caaatcagct tcgtccattc gcgctatcgt aatgaaaacg
     1741 tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg
     1801 agatggcaca agcagcggaa caggcgagtc agtcaaaatc gatgttcctt gccaccgtca
     1861 gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa cctggatctg ttgcaaacca
     1921 aagagttacc gaaaggcgtc gatcggctgg tgacggcaat gaacaactct tccagcctgt
     1981 tgttgaaaat tatcagcgat attctcgatt tctcgaagat tgaatcggaa cagttgaaga
     2041 tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca catcaccgcc aactatttac
     2101 cgctggtggt acgcaagcag ttaggcttgt actgctttat tgaaccggat gtgccagtgg
     2161 ccttaaatgg cgacccgatg cgtttacagc aggtcatctc caacctgttg agtaacgcca
     2221 taaaattcac cgataccggc tgtatagttt tgcatgtccg cgctgatggc gattatctct
     2281 ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa agaagtggtg cgcttgtttg
     2341 atcccttctt ccaggtcgga acgggcgtac agcgaaactt ccaggggacc ggtctgggtc
     2401 tggcgatttg tgaaaaactg atcagcatga tggacggcga tatctcggta gattcagaac
     2461 cgggaatggg cagccagttt accgtgcgta ttccgttgta cggcgctcag tacccgcaga
     2521 aaaaaggcgt ggaagggttg agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc
     2581 tctgtcagtt cctagaaact agtttgcagc gcagcggcat cgtcgttaca acatacgaag
     2641 ggcaggaacc gactcccgaa gatgtgttaa ttactgacga ggtagtgagt aaaaaatggc
     2701 agggtagagc ggtagtgacc ttctgtcgtc gccatattgg tattccgctg gagaaagcgc
     2761 caggggagtg ggtacacagt gtggctgccc cgcatgagct accggcattg ttggcgcgta
     2821 tttatttgat cgagatggag agcgacgatc ctgctaacgc tctgccgtcg acggacaaag
     2881 cggtcagcga taatgacgat atgatgattc tggtcgtgga tgatcatccg attaaccgac
     2941 gtctgctggc agatcagttg ggatcgttgg gctatcaatg taaaaccgcg aatgatggtg
     3001 tcgatgcgct taatgtactt agcaagaatc atattgatat cgtgcttagc gacgtcaaca
     3061 tgccaaatat ggatggttac cgcttgacgc aacgcattcg tcagttggga ctgacgttgc
     3121 cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg
     3181 gtatggacag ctgcctgtcg aagccggtaa cgctggatgt gataaaacag acgctgacgg
     3241 tatatgccga gagggtcagg aaatcgcggg aatcgtagga cggataaggt gtttatgtcg
     3301 catctggcat tcagtgcaaa tgccagatgc gacgctgacg cgtcttatct ggcctacttt
     3361 gatgtaatct gttgaaataa tgggaatcgt aggacgcata aggcgtttac gccgcatccg
     3421 gcaatcggtg caaatgctag ataagacact aacgcgtctt atctggccta caggtgatta
     3481 gtctttatct gccggactta acgtcactga agagagataa ttcagcaggg cgatatcgtt
     3541 ctcgacaccc agcttcatca tcgcagattt cttctggcta ctgatggttt taatactgcg
     3601 gttcagcttt ttagcgatct cggtcaccaa gaagccttcc gcaaacaggc gcagaacttc
     3661 actctctttt ggcgagagac gcttgtcacc gtaaccacca gcactgattt tttccaacag
     3721 gcgagaaacg ctttccgggg taaatttctt ccctttctgc agcgcggcga gagctttcgg
     3781 cagatcggtc ggtgcacctt gtttcagcac gatcccttcg atatccagat ccaataccgc
     3841 actaagaatc gccgggttgt tgttcatagt cagaacaatg atcgacaggc ttgggaaatg
     3901 gcgcttgatg tacttgatta aggtaatgcc atcgccgtac ttatcgccag gcatggagag
     3961 atcggtaatc aacacatgcg catccagttt cggcaggttg ttgatcagtg ctgtagagtc
     4021 ttcaaattcg ccgacaacat tcacccactc aatttgctca agtgatttgc gaataccgaa
     4081 caagactatc ggatggtcat cggcaataat tacgttcata ttgttcatgt aataggctac
     4141 cttgctacag caagctcttg acataactgt caatgtcgct gatgtatttt tctattcctg
     4201 gaacatcctt ctcacgaatc agatgttcca gcgtttcaca taactgcttg ccgggtacca
     4261 gatttagcat ggcaaatacg cctttaagac gatgagccgt ttgggctaac gcagcaaagt
     4321 cactggttgc tgcttcagta tacagcctct taacatcatc cggtactgtg tctacaaaga
     4381 gcgcataata gccgctggca tggagttgcg cattttcatc tccgcccaga ggcgattctg
     4441 tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt
     4501 tgctcatatt gaagttgacg cacaattgac caggcccaat ttcccgtacg ccagactcat
     4561 catcgcttaa aagcaagcca gaggcagtaa gattagacgg attatccgtt aaaaagatat
     4621 cataatcttg actaattaat ctttcatcgg gtgtgataca ggttgcaccc caattttcta
     4681 actggcgagt gacaatattc cgaatttctg ccgaagtaac atccaccatt acgcagacat
     4741 catccagtaa acgctcttct tcctcttcaa cttccgggtc agcggcgagc attttgatat
     4801 gcacagagta gcgtgtacca agcccatccc gcgttttgat gtttaaatga ccgcccagtt
     4861 tacgcgccag ttgatcgctc agccagaatg ccagcgggtc cgccttgcca tagcgatcgt
     4921 tttggggctg gttgataaac gggaagtgca aattatccat ttcatgaata cttacgcctt
     4981 ctcccgtgtc cagaatgcgg aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct
     5041 caagggtgat ttttcccaat tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa
     5101 tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa
     5161 tcagcagttg cagaccttta cgcttgatgg caggcaacac tgaaggcaca acttcatcaa
     5221 ttaaatcctg cacggagaac agcaccgtct cacttttcca gctatcgtcc gcaagcatgt
     5281 tcgctaactg tatttcatcg accagccgca ccagcacatc tgcctgattc gccagttgtt
     5341 tgctttccgg ggcgttgagt ttagccgcgc tctccgccag ggactgtgcg ggttctttca
     5401 gcgcatcgcc aatgtttttc ataaaggtca tccgcccctg ctggtttttc tcatacagac
     5461 gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg atcctgatcg cgaataatga
     5521 aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg gatctcatac agctcgttat
     5581 tgatcgtcgc ctgaataatc ccctgatgct gttccgccat ggtggtgatg ttttgcagat
     5641 tcaaatgcgg cagcaaatga tcggcaattt tgttacttat gacagtgcgg ttcgattcct
     5701 gatcgtgaac cagcaggccg agcggcagca gtgagactat ctcttcatta attgcccgta
     5761 aaatgcgcaa ttcgttattg accgccgtgc tgggtagact ttctgtactg cggctggaga
     5821 aatggcggaa tgtggtatag ccaaataacg ccagcgccag caaaccgatg ttcagcagca
     5881 gtggcagcag aatgttttgc aacgtatcca gcaataaggt gccataagga acctgccaga
     5941 ccaggcgcat atcggtagag ttgagtgccg aggagatttc aatcttcgta ctgttaaagt
     6001 ggatactgac gctatccgtc ccttctttct cattatcatt gtctcccgtt gccgtcgcgt
     6061 ctggctcaag gcggaaactg tccagcggca tacccggtgg gatcaaatca ttaatcggta
     6121 gatcaaaagc tacgaccgtt gccagatgtc ccggctggtt gaacgtggta cgcaaggtaa
     6181 agtaatgacc gttctgccag gccaggcggc gcatgttaga aaaactttcg cgttcatcga
     6241 gggcgttggc ctgttgcaac atctctgcac gacgtgaatc aacaatgtcg ctgatggtcg
     6301 attctttaaa tccggaggtg agatctttga ggggcagggt tgagatcagc accagactgt
     6361 tatcctgacc attcaggtaa tacatcgacc acggtacatt ttctgcgccc cacaatgtgt
     6421 ccagataggt ggacatccgc tgagtcatct caagcgttga gttgtcgtga gagccaaaga
     6481 ttaacgcttc cgttttgcgg cgcggtttct ccagatagta gacatcctgt ttcaggcgcg
     6541 tctcttgtaa accttcgccg gaggaggggg aggttgtcgc ggcaatgttg tcgtagatct
     6601 gccaggtcac gtaacgccag gtattgacgc gcttttggat agcgtgggta atgtcgacaa
     6661 tctggtaact tttatctttc agccatgcgt taacggcgct ttgtaccatt acacccatcg
     6721 tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg
     6781 aaaagcgggt cgtggccgtt gtctctttct gacgcattcg tgtttatgac ctgttaaaac
     6841 ttcgcgaagt gatagggcag tataaagggt acagtgtgaa attccagact cttacctgat
     6901 tgatgattct ctattctccc gtgatattta tcatcctgca ttgggggaaa tagatgtaca
     6961 agcgccattt tgctgtacaa ataacctaca aaaagcccaa cgaaattaat tgcaaataaa
     7021 cgatgattgc tggaaattat gctgatgtta attatttgtg aaatagttaa caagcgttat
     7081 agtttttctg tggtagcaca gaataatgaa aagtgtgtaa agaagggtaa aaaaaaccgg
     7141 atgcgaggca tccggttgaa ataggggtaa acagacattc agaaatgaat gacggtaata
     7201 aataaagtta atgatgatag cgggagttat tctagttgcg agtgaaggtt ttgttttgac
     7261 attcagtgct gtcaaatact taagaataag ttattgattt taaccttgaa ttattattgc
     7321 ttgatgttag gtgcttattt cgccattcca caataatctt aaaaagttcc cttgtattta
     7381 cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt
     7441 gttggattat tctgcatttt tggggagaat ggacttgccg actgattaat gagggttaat
     7501 cagtatgcag tggcataaaa aagcaaataa aggcatataa cagagggtta ataacatgaa
     7561 agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag caaacgctgc
     7621 tgaagtttac aacaaagacg gcaacaaatt agatctgtac ggtaaagtag acggcttgca
     7681 ctatttctct gacaacaagt cagaagacgg cgaccagacc tatgtacgtc ttggtttcaa
     7741 aggcgaaact caggttactg accagctgac cggttacggc cagtgggaat atcagatcca
     7801 gggcaatacc tctgaagaca acaaagaaaa ctcctggacc cgtgtggcat tcgcaggtct
     7861 gaaattccag gatgtaggtt ctttcgacta cggtcgtaac tacggcgttg tttacgacgt
     7921 aacttcctgg accgacgtac tgccagaatt cggtggcgac acctacggtt ctgacaactt
     7981 catgcagcag cgtggtaacg gcttcgcgac ctaccgtaac accgacttct tcggtctggt
     8041 tgacggtctg aactttgctg ttcagtacca gggcaaaaac ggtagcgtaa gcggcgaagg
     8101 catgaccaac aacggtcgtg gcgctctgcg tcagaacggc gacggtgtcg gcggttctat
     8161 cacttatgat tacgaaggct tcggtatcgg tgctgcagtt tccagctcca aacgtactga
     8221 tgatcaaaat ggtagctaca tcagcaatgg tgtagttcgt aactacatcg gtactggcga
     8281 ccgtgctgaa acctacactg gtggtctgaa atacgacgct aacaacatct acctggctgc
     8341 tcagtacacc cagacctaca acgcaactcg cgtaggttcc ctgggttggg cgaacaaagc
     8401 acagaacttc gaagctgttg ctcagtacca gttcgacttc ggtctgcgtc cgtctgtagc
     8461 atacctgcag tctaaaggta aaaacctggg tgtcatcaat agtcgtaact acgacgacga
     8521 agatatcctg aaatatgttg atgttggcgc gacctactac ttcaacaaaa acatgtccac
     8581 ctatgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg acgctggcat
     8641 caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc gattgatatc
     8701 gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaactc agttttttgg
     8761 tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct ttacccgcgt
     8821 ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac aacccgccaa
     8881 aacccacgct actgaagtta ccgttcttga aggcaaaacc atgggtacct tctggcgtgc
     8941 cagcatcccg ggcattgatg ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca
     9001 gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt
     9061 taacgactcg caaagtttgt cgccgtggcc agtaagtgaa gcgatggccg aaatcgtcac
     9121 tacctcgctg cgcattggcg cgaagaccga tggtgcgatg gatattaccg tcgggccgct
     9181 ggtaaatctg tggggctttg gcccggaaca acagccggtt caaattccga gccaggaaca
     9241 gatcgacgag atgaaagcca aaactggttt acagcacctt acggtcatta atcagtcgca
     9301 tcagcaatat ctgcaaaaag acctgccgga tttatatgtc gatctctcta ccgtcggcga
     9361 aggttatgcg gcggatcact tggcacgctt gatggagcag gaagggattt cccgctatct
     9421 ggtgtcggtg ggcggcgcgc tgaacagccg tggtatcaac ggtgaaggcc tgccgtggcg
     9481 ggtagcgatt caaaaaccaa ccgataaaga aaacgcagtt caggctgtgg tggatatcaa
     9541 cggtcatggg attagcacct ctggcagcta ccgtaactat tacgaactgg acggcaaacg
     9601 tctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt
     9661 gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct tgatggtact
     9721 cgggccggag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt atatgatcac
     9781 caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct tccttgtcag
     9841 cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg gcagactaaa
     9901 ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact gacgatcaac
     9961 gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt ttcgccgtgc
    10021 gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgccttg cgggaaaatg
    10081 tctccttcta cgcaaatgcc agcgaggcac tcgccgccgg atttcgcccc tgcaaacgct
    10141 gccaacctga taaagccaat gcccagcaac atcggttgga taaaatcacc cacgcgtgtc
    10201 gactgctgga acaggaaacg cctgtaacgt tggaagcctt agccgaccag gtggcgatga
    10261 gtccatttca tctacatcgg ttgtttaaag cgactaccgg aatgacgcct aaagcctggc
    10321 aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga
    10381 cgtctattct taacgccgga ttccccgaca gcagcagtta ctaccgcaaa gccgatgaaa
    10441 cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg gtgcgttacg
    10501 cgctggctga ttgtgcgctg ggtcgttgcc cggtggcaga aagcgagcgg gggatttgcg
    10561 cgatattgct gggcgatgat gacgcgacat taatcagcga gttgcagcag atgtttcccg
    10621 ctgccgacaa cgcgcctgcc gatctgacgt ttcagcaaca tgtgcgtgaa gtgatcgcca
    10681 gcctcaatca acgcgatacg ccgctgacgt taccgctgga tattcgcggc actgcttttc
    10741 agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgta ctggctaacg
    10801 ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg
    10861 ctatcgtaat accctgtcat cgagtggtcg gtggtgatgg cacactttcc ggttaccgct
    10921 ggggcgtgtc tcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag gagaggtaat
    10981 gttggatctg tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat
    11041 tttacggcgt tttgctttta acgctgcgga gcaactgatc cgcgatatta atgacgttgc
    11101 cagccagtcg ccgtttcgcc agatggtcac ccccggggga tataccatgt cggtggcgat
    11161 gaccaactgt gggcatctgg gctggacgat ccatcggcaa ggttatctct attcgcccat
    11221 tgatccgcaa acaaataaac cgtggcccgc catgccacaa agctttcatg atttatgtca
    11281 acgtgcggct acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg
    11341 ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc
    11401 gccaattgtt tctgtttctc tgggcttacc cgcgattttt caatttggtg gcctgaaacg
    11461 taatgatccg ctcaaacgtt tgttgttgga acatggcgat gtggtggtat ggggcggtga
    11521 atcgcggctg ttttatcacg gtattcaacc gttgaaagcg gggttttatc cactcaccac
    11581 cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt
    11641 attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt
    11701 ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat
    11761 ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta tcaatcagcg
    11821 ccttatcgaa atggcggata ccagtctgct ggtattgccg gagtttctgg gattattgct
    11881 gctgttgatg gcagtcactc tcggatcgca actggcgctc accactttgg ggcatcactt
    11941 cgtttaccga ctgcgtagtg aatttatcaa gcggattctg gatacccacg tcgagcgcat
    12001 tgaacaactc ggtagcgcct cgttgttggc gggattaacc agcgatgtgc gcaatatcac
    12061 cattgctttt gtgcgtcttc cggaactggt gcaggggatc attctcacta tcggttccgc
    12121 ggcgtatctg tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcgat
    12181 caccatctgg ggtggttttg tactggtggc gcgggtgtac aaacatatgg cgaccctacg
    12241 tgaaaccgaa gacaagctgt acacggattt tcaaactgta cttgaagggc gcaaagagct
    12301 gactctgaac cgggaacgcg ccgagtatgt gtttaacaac ctctacatcc ccgatgcgca
    12361 agagtatcgc catcatatta ttcgcgcaga caccttccat cttagtgccg tgaactggtc
    12421 aaacatcatg atgctgggcg ctatcggcct ggtgttctgg atggcgaaca gtctcggttg
    12481 ggctgatacc aacgttgctg cgacctattc gttgacgctt ttattcctgc gtacgccgct
    12541 gctttcggcg gttggcgcat tgccgacgct gctgatggcg caggtggcgt ttaacaagct
    12601 gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg cgtttcccaa
    12661 ctggcaaacg ctggagctgc gtaatgtgac gtttagctat caggataacg cgttttccgt
    12721 tggtccgatt aatctcacca tcaaacgtgg cgagctactg tttctgattg gtggtaacgg
    12781 tagtggaaaa tcgacgctgg cgatgttgtt gacgggcttg tatcagccac aaagcggcga
    12841 aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt
    12901 ttcggcagtg tttaccgatg tctggctttt tgatcaactg ctggggccgg agggtaaacc
    12961 cgctaacccg caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga
    13021 gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc
    13081 gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga
    13141 tcaggatcca cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat
    13201 gggtaaaact attttcgcta tcagtcatga tgatcattac tttatccacg ccgaccgcct
    13261 gctggaaatg cgcaatgggc aacttagcga gctgacgggc gaagagcgcg atgccgcttc
    13321 gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg
    13381 tcaggtcaat atgctaaaaa tcccttcatt tctaataacc ctataattaa tcaacgaaat
    13441 tataatgttt ctaaaattag aatataattt ataaacatta tttaaatgtt gttacttaag
    13501 tgttaccttt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta
    13561 acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt
    13621 cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg
    13681 gcggcattat gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga
    13741 tgaccatgct ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaataacg
    13801 ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca
    13861 gtatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct
    13921 gggcgcacca ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc
    13981 cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg
    14041 cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca
    14101 aagagtgggc accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc
    14161 gtacggaaat tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct
    14221 tgcagaagaa atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta
    14281 atgacgataa cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca
    14341 ttcgtgccaa atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat
    14401 cggggattcc ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt
    14461 cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg
    14521 gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag
    14581 tgctgtttgg gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg
    14641 atctgatgag ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata
    14701 atttcgatct ggtgaaatat ctggtgagtc aggtgatgtt gagtgaagag gatcgttttg
    14761 aagcgttgaa agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg
    14821 ggcagcgtgt gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta
    14881 ctgaagtggt cagtgaccag caagggacca ttgccgcgct cctgggagca tcgccagggg
    14941 cgtcaaccgc cgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt
    15001 ccagcccgca atggcaggct acgttgaaag cgatcgttcc gtcttatgga cgcaagctga
    15061 acggtgatgt agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctggggctga
    15121 aatacgacaa gccgcaggca gcagatagta cgccaaaacc gcagttgaaa ccgcaacccg
    15181 ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc
    15241 ggatgtggcg tatactgata agacgcgcca gcgtcgcatc aggcaaccgg ctcgggcgtt
    15301 agatgttgga tgcggcgtaa ccgccttatc caaccaacgg ggtgccgtac ttcacaacag
    15361 tttagcgaac ttctgcgttg tcaattttct cttccgcctt ccagacgcgg tatttcacat
    15421 ctacattgtc tggcgtatac accacgatcg gcagcttgct gttgtaacgc agcattccag
    15481 catcgcccag atacgcggtg acaaatttct tctctttctt gccatccggg caggccatca
    15541 tcgtggaaac cggcgagcta actttatcaa agacataata atcatagccc cagccttcca
    15601 gcgttttgct ttccagcttc ccgccgagac gatgcaaatt gcaatcgact tccagcgtct
    15661 gaccgattaa cagttctact ttcagggtag attcatcttc ttgcggggtt aactgaatca
    15721 cctgacgctt catccctttt tcagcttgtg gataaggcgc gattttttcc agtggctgga
    15781 cgctttctgc cgcccaggcg gaagtggtag cgaaagcggc aaacaatact gcaggtagaa
    15841 tggtcttcat ttttttgctt tccttcttca tttataggtt ttcaccgatg atgtcgctga
    15901 aggttaacat attcataaga tttattaatt tgctttatga ataacttaaa gaatattcag
    15961 acaattttac ctgctctttt agtgttaaat tctgatgaga gaacaaacca gcagaaatat
    16021 ctctggtgaa ttaatcgcta tataaatatg tttataacca tttgaaatgt gagcaaaagc
    16081 ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta ccccgatcgg
    16141 ggtatgcatc tttgacacat cctttaatat cttagcggtt ataaaaatgg cttattaatt
    16201 atgcggttta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa ggtcaatgtg
    16261 aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc cagtaacggt
    16321 atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac ccgttgcgac
    16381 gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg ctatccgagc
    16441 gttaatttca aaaataacga gtgcagcttc tgttacgctt gcgcgcaggc ttgccccgaa
    16501 tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat tggggacgcg
    16561 tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga gccaatggca
    16621 attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag ccaactttgc
    16681 aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc ggagtatctc
    16741 catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg aacgaatttc
    16801 agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca gcgacgcgcc
    16861 gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga tccaaaccat
    16921 tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc accagcagga
    16981 agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa agctaacgcc
    17041 gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg cgccgttgtt
    17101 ggtcagcagg aagccatcaa atgggataaa gcgccgtgcc gtttctgcgg tactggttgc
    17161 ggcgttctgg tcggaacgca gcagggacgt gtggtggcct gtcagggcga cccagacgca
    17221 ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat catgtacggt
    17281 aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga caaagaaggc
    17341 gaatttaccc taatcacctg ggatcaggcc ttcgatgtga tggaagagaa attcaaaacc
    17401 gccctgaaag aaaaagggcc ggaatcgatc ggtatgttcg gttctggtca gtggactatc
    17461 tgggaaggtt atgccgcgtc caagctgttt aaagcgggct tccgttcgaa caacatcgac
    17521 ccgaacgcgc gtcactgtat ggcgtcggca gtagttggct ttatgcgtac ctttggtatg
    17581 gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt gctgtggggc
    17641 gcaaacatgg cggagatgca cccgatcctc tggtcgcgca tcactaaccg tcgtctctct
    17701 aatcagaacg tcaccgtggc ggtgctttct acctaccagc atcgtagctt cgagctggcg
    17761 gataacggca tcatctttac gccacagtct gacctggtga tcctgaacta catcgccaac
    17821 tatatcattc aaaacaatgc gataaatcag gacttcttca gcaaacacgt taacctgcgc
    17881 aaaggggcga cggacattgg ctacggttta cgtccgaccc atccgctgga aaaagcagcg
    17941 aagaatccag gttctgacgc ctccgaaccg atgagctttg aagattacaa agccttcgtt
    18001 gccgagtata cgctggaaaa aactgccgaa atgaccggcg tgccgaaaga ccagttagaa
    18061 caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg gacgatgggc
    18121 ttcaaccagc atactcgtgg cgtgtgggct aacaacctgg tctacaacct gcacctgctg
    18181 accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca accttctgcg
    18241 tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga catggtggtg
    18301 accaacgaga aacaccgtga tatctgcgag aagaagtgga atatcccgag cggcaccatt
    18361 ccggcgaaaa tcggtctgca cgcggtggca caagaccgtg cgctgaaaga cggcaaactg
    18421 aatgtttact ggaccatgtg taccaacaac atgcaggccg ggccgaacat caatgaagag
    18481 cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc gtatccgaca
    18541 gtcagtgcgc tggccgccga cttgatcctg ccgaccgcaa tgtgggtaga gaaagagggc
    18601 gcttacggta acgccgaacg ccgtactcag ttctggcgtc aacaggtaca ggcaccgggc
    18661 gaagcgaaat cggatctctg gcagttagtc cagttctccc gccgcttcaa aactgaagaa
    18721 gtatggccgg aagatctgct ggcgaagaaa ccggaactgc gtggcaaaac gctgtacgaa
    18781 gtgctgtatg ccacaccgga agtgagcaaa ttcccggtat ccgaactggc ggaagatcag
    18841 ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt cgaagagtac
    18901 gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt tcgatgacta ccacaaagcg
    18961 cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc agtggcgtta cagcgaaggt
    19021 aacgacccgt acgtgaaagc gggcgaaggc tataagttct acggcaaacc ggatggcaaa
    19081 gcggtgatct tcgcactgcc attcgaaccg gcggcggaag caccggatga agagtacgac
    19141 ctgtggctct ctaccggacg cgttctggag cactggcaca ccggcagtat gactcgccgt
    19201 gtaccagaac tgcaccgcgc cttcccggaa gcggtcctgt ttattcaccc gctggatgcg
    19261 aaagcgcgcg atctgcgtcg tggcgacaaa gtgaaagtgg tttctcgccg tggcgaagtg
    19321 atctcgattg tcgaaacgcg cggtcgtaac cgtccgccgc agggcctggt gtacatgccg
    19381 ttcttcgacg ccgcacagct ggttaacaaa ctaacgcagg acgcgaccga tccgctctcg
    19441 aaagagacgg acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc cattatgtcc
    19501 cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgttgt tcgcacagca
    19561 ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc acgcgcatct
    19621 ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag tgcctgtgtg
    19681 cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc gacgctggcc
    19741 tctggtctgt cggcgggcac gccatatttt gtcgcacggg atattccttg cgaaatgtgt
    19801 gaggacattc cgtgcgccaa agtgtgcccg agcggtgcgc tggatcgtga gatcgaatcg
    19861 atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg tctcaacttt
    19921 caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga ggccatcacc
    19981 ctggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc gacggttcac
    20041 agagacgcct gtaccggttg cggtaagtgc gaaaaagtgt gcgtgctgga acaaccggca
    20101 atcaaggtgt taccgctgtc actggctaaa ggggagttag gtcaccatta ccgcttcggc
    20161 tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg cgctggagaa
    20221 aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc agttcttcgt
    20281 gctggggatg tttttgagcg gtccgtggtt tggtgtgtgg atcttacacg gtaactacag
    20341 cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc tgcaaagtct
    20401 tgccagcggt catctgcctg ccacggtggc gttgaccggt gcggtgatta tcaccgtgct
    20461 ctacgccctg gcaggaaagc gattattttg cagctgggtc tgcccgctga atccgattac
    20521 cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga cgatccctcg
    20581 ccacattcgc tacgtgctgt tggtggtcat tctgctggga tcggccttaa ctggcacgct
    20641 catctgggaa tggatcaacc ccgtatctct gatggggcgc agcctggtaa tgggcttcgg
    20701 cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg ttgaacacgg
    20761 ctggtgcggg cacatttgtc cggttggcgc gctgtatggc gtgctgggta gcaaaggtgt
    20821 gattaccgtt gcggccaccg atcggcagaa atgtaaccgc tgtatggatt gttttcatgt
    20881 ttgcccggaa ccgcgtgtgc tacgtgcccc ggtgctggat gaacaaagcc cggtgcaggt
    20941 caccagccgc gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg aggatgtatt
    21001 tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga cctgaagaaa
    21061 gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt ttgggcggct
    21121 aatggcgtcg attttagcca gtcgccggaa gtatccggga cacaggaagg ggccattcgg
    21181 atgccaaaag agcaggaacg gatgccgctg aactatgtga atcagccgcc aatgatcccg
    21241 catagcgttg aaggttatca ggtaacgacc aataccaacc gctgcttgca gtgccacggt
    21301 gtcgaaagtt atcgcaccac tggcgcgccg cgcattagtc ctactcactt tatggacagc
    21361 gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca atgtcacgta
    21421 ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa aggttacggg
    21481 aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa gcgcctgtgg
    21541 aaatggtggc gtacccccag ccgtctggcg ctggggacgc tgctgttgat cggttttgtt
    21601 ggcggcatcg tcttctgggg tggctttaac accgggatgg aaaaagccaa taccgaagag
    21661 ttctgcatta gctgccacga aatgcgcaac acggtgtatc aggaatacat ggattccgtg
    21721 cactacaaca accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt tccgcacgag
    21781 tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg taaaattttt
    21841 ggcgttattg acacgccgca gaaatttgaa gctcatcgtc tgacgatggc acagaatgag
    21901 tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa cttcgagtat
    21961 atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc ggtgaaagat
    22021 gggcaaacct gtattgattg ccataaaggg atagcgcaca agctgcccga tatgcgtgaa
    22081 gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagccag agagttactt
    22141 tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa cgcaggagag
    22201 tgggtacaaa tcaccggtag caacggcgcg gggaagacaa cgcttctccg tttgctgacg
    22261 gggttgtctc gccctgacgc aggcgaggtt ctctggcaag ggcagccctt gcatcaggta
    22321 cgcgacagct accatcaaaa cctgttatgg ataggccatc agccggggat caaaacccgg
    22381 ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgataccgc acaatgtctg
    22441 gaagccctgg cgcaggccgg gcttgccgga ttcgaagata ttcctgtaaa tcagctctcg
    22501 gccgggcaac aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc cacgttatgg
    22561 atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct gacccagcgt
    22621 atggcgcagc atacggagca gggggggatt gtgattctga ctacccacca gccgctcaac
    22681 gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgagggccgc atgatgttct
    22741 ggcgcatttt ccgtcttgag ctgcgtgtag cgtttcgcca tagcgccgaa atcgccaacc
    22801 cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt ccggagccgc
    22861 aactgctggc gcgtattgca ccgggcatta tctgggttgc tgcgctgctt tcatccttgc
    22921 tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa caattgatgt
    22981 tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac tggatggtaa
    23041 ccggtctgcc gttactcatc ctttcgccac tggtagcaat gctactggga atggatgttt
    23101 atggctggca agtgatggcg ctgacgctgc tgctgggaac gcctacgctt ggctttctcg
    23161 gtgcaccggg tgtggcgttg acagtgggac ttaagcgcgg tggtgtgctg ctcagcatac
    23221 tggtgttacc gctgactatc ccattactca tctttgccac cgccgcgatg gacgcggctt
    23281 ccatgcattt gcccgttgac gggtatctgg caattttagg cgcgttgctg gcaggcaccg
    23341 cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa taacgctgga
    23401 ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc atcaactggc
    23461 gatcccacca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg caattgccag
    23521 tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg ctgattatca
    23581 gcagggaaat agctaccgca ttatctacct gcatgtgcct gcggcgatct ggtcgatggg
    23641 tatttatgca tcaatggcgg tggcggcgtt tattggcctt gtctggcaga tgaaaatggc
    23701 caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta ttgccctggt
    23761 taccggctct gcatggggaa aaccgatgtg gggcacctgg tgggtatggg atgcacgtct
    23821 gacttctgaa ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt ggcacgcctt
    23881 cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg gcgtggtgaa
    23941 tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg gatcaacgcg
    24001 gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga tttttggctt
    24061 cctgctcctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt tgctgatgga
    24121 aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat gacccttgca
    24181 tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt ctggctggcg
    24241 gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat gcagcatcgc
    24301 gcaattctgt gtggtgtgga gcaacagcgg gcgcgtgagg cgcgtttacg tgctgcgcaa
    24361 cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc tgtgctgtgt
    24421 tggcaggttt ggcgctgacc atcggtctgg tgctgtatgc gctgcgctcg aatatcgatc
    24481 tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcaa atgccggaag
    24541 tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg tagtgtgcag cgcgatccca
    24601 attcgctgaa agtgaccttc accatttacg acgctgaagg ctcagtggat gtctcttacg
    24661 aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag ggcgagctgg
    24721 aaaaaggcaa tcatatcctc gcgaaagaag tgctggcgaa acacgacgaa aactacacgc
    24781 cgccagaagt tgagaaagcg atggaagcta accaccgtcg cccggcgagt gtttataagg
    24841 acccagcatc atgatgccgg aaattggaaa cgggctgctg tgcctggcgc tgggaattgc
    24901 gctgctgctg tccgtgtatc cgctatgggg cgtggcgcgc ggagatgcgc gcatgatggc
    24961 gtcttcccgc ttgtttgcct ggatgctgtt tatgtctgtg gctggcgcat ttctggtgct
    25021 ggtcaatgct ttcgtggtca acgactttac cgtcacctat gttgccagca actccaatac
    25081 ccagcttccg gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag gttcgcttct
    25141 gctgtgggtg ctgctgatga gcggctggac ctttgcggtg gcgattttta gtcagcgtat
    25201 tccgctggat attgtggccc gtgtactggc gataatgggg atggtcagcg tcggcttttt
    25261 gctgttcatt ctctttacct ctaacccgtt ctcacgcacg ctgccgaact tcccaattga
    25321 agggcgcgat cttaacccgc tgttacagga tccggggctg atcttccatc cgcctctgct
    25381 ttatatgggg tacgtgggtt tctcggtggc gtttgctttt gccattgcca ttgcttcttt
    25441 gctgagcggg cgtctggaca gcacttatgc gcgttttact cgcccgtgga cgctggcggc
    25501 gtggattttc ctgacgctcg gcattgtgct cggttccgca tgggcctatt acgaactcgg
    25561 ctgggggggc tggtggttct gggatccggt agaaaacgcc tcgtttatgc cgtggctggt
    25621 ggggactgcg ctgatgcact cactggcggt cactgaacaa cgcgccagct tcaaagcgtg
    25681 gacattactg ctggcaatca gtgccttctc gttgtgtctg ctggggactt tcctggtgcg
    25741 ttccggcgtg ctggtatcgg tacacgcgtt tgcctctgat ccggcacgcg gtatgtttat
    25801 cctcgccttt atggtactgg tgattggtgg ttcgctgctg ctgtttgccg cgcgtggaca
    25861 caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg gaatctctgc tgttagcgaa
    25921 caacgttttg ttggtcgccg cgatgctggt ggtattactg gggacgctgc tgccgctggt
    25981 gcacaagcaa ctgggactgg gcagtatttc gattggcgaa ccgttcttca acaccatgtt
    26041 tacctggctg atggtgccgt ttgcgctgct gcttggtgtc ggtcctctgg tgcgctgggg
    26101 gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc gccttcatct caacgctggt
    26161 gctgtcgctg ctgctgccgt ggctgttcga aagcaaagtt gtggcgatga cggtgctcgg
    26221 cctggcaatg gcctgctgga ttgctgtgct ggcaattgcg gaagctgcgc tgcgtatttc
    26281 acgtggcacg aaaaccacct tcagttattg gggaatggtg gcggctcacc tggggctggc
    26341 agtgacaatt gttggtattg cctttagcca gaactatagc gttgagcgtg atgtgcgcat
    26401 gaagtccggc gatagcgtcg atattcatga atatcgcttc accttccgtg atgtcaaaga
    26461 ggtgactggc ccgaactggc gcggcggtgt ggcgactatt ggcgtaacgc gagagggtaa
    26521 accggaaacg gtgctgtatg cggaaaaacg ttattacaac actgccgggt cgatgatgac
    26581 cgaagcggcg attgacggcg gcatcacgcg tgacctgtac gccgcgctcg gtgaagagct
    26641 ggaaaacggc gcgtgggccg tgcgtcttta ctacaaaccg tttgttcgct ggatttgggc
    26701 gggcgggctg atgatggcgt tgggcggact gctgtgtctg tttgatcctc gctatcgtaa
    26761 gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga agcgcaaagt attgttaatt
    26821 ccgttgatta tcttcctggc gattgccgcg gcgctgctgt ggcagctggc gcgtaatgcc
    26881 gaaggggatg atccgaccaa tctggaatcg gcgctcattg gcaagccagt gccgaagttt
    26941 cgcctcgaat cactggacaa tccggggcag ttttatcagg cggatgtgct gactcagggc
    27001 aaacctgtgc tgcttaacgt ttgggctacc tggtgtccga cctgccgtgc ggaacatcaa
    27061 tatctgaatc agctttctgc gcagggcatt cgcgtggtcg gcatgaacta taaagacgat
    27121 cgccagaagg caataagctg gctgaaagag ctgggcaatc cttacgcgct aagcctgttt
    27181 gatggcgatg gcatgttagg gctggatctc ggtgtctatg gcgcgccaga aacgttcctc
    27241 atcgacggca acggcatcat tcgctatcgc catgcgggcg atctcaatcc tcgcgtctgg
    27301 gaagaagaga tcaagccgct gtgggagaaa tacagtaagg aggccgcaca atgaggtttt
    27361 tattgggcgt gctgatgctg atgatctccg gctcagcgct ggcgaccatc gacgtgttgc
    27421 agtttaagga tgaagcgcag gagcagcagt tccgccaact cactgaagaa ctacgctgcc
    27481 cgaaatgcca gaacaacagc attgccgatt ccaactcgat gattgccacc gacctgcgcc
    27541 agaaagtgta tgaactgatg caggaaggta aaagtaaaaa agagattgtc gattatatgg
    27601 tggcgcgtta cggcaacttc gtcacttacg atccgccgtt aacgccgctg accgtgctgc
    27661 tgtgggtgct tccggtagtg gctattggca ttggcggttg ggtcatttac gcccgttcgc
    27721 ggcgtcgggt acgcgtggtg ccggacgcgt ttcctgaaca aagcgtgccg gaaggtaagc
    27781 gtgccggata tattgtttat ctgccgggta ttgtggtggc gttaattgtg gctggcgtca
    27841 gctactacca gactggcaat tatcagcagg tgaaaatctg gcagcaggcc acggcacagg
    27901 ctccggcgtt gctggacagg gcgctggatc cgaaagccga tccgctcaac gaagaagaga
    27961 tgtcgcgtct tgcgctgggg atgcgtactc aactgcaaaa aaatccggga gatatagaag
    28021 gctggattat gttgggccgc gttggcatgg cgctgggtaa cgccagtatc gccaccgatg
    28081 catacgctac tgcgtatcgc ctcgatccga aaaacagtga tgccgcactg ggttatgctg
    28141 aagcgttgac acgttcatct gatcccaacg acaaccgcct cggtggtgaa ctgctgcgcc
    28201 agttggtgag aacggaccat agcaatatcc gtgtgttaag catgtatgcg tttaatgcct
    28261 ttgagcagca gcgatttggc gaagccgttg ccgcgtggga gatgatgttg aaactcttat
    28321 ctgccaacga tactcgccgt gcggtgattg aacgtagtat cgcgcaggcg atgcaacatt
    28381 tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg atgccatcgg gctattttta
    28441 ttgtgccccg cgttgttgca ggaacagaat ggtggctgcc acgcgtgagc ggacattgag
    28501 tttgcgcagc agattgcgaa tatgtacttt tactgtctgc tctgaaatat tcaacaccga
    28561 ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc agaacatcca gctcgcgctc
    28621 cgtcagcacg ctgaagggat cttcttccgc gccaaacatt tcacgttcac gtaagtactg
    28681 attgacgcgt tcgctaaaga ctttgctgcc tttcgctcca gcacgaattg cttccagcaa
    28741 tacttccggg tcgctgtctt tcaacagata accgtctgcg cctgcgtcta tcagcgcaaa
    28801 gacatcgctg gaggcatctg atacggtcag gataataatt tgcgcggtaa cgccatccct
    28861 gcgcaaggca ttgagggtat ccaggccact catacctttc atattgagat ccagcaagat
    28921 cacgtcgata tccagtctat ttgccagatc gatcgcgctc gcgccgtcgc ccgcttcggc
    28981 gaccacttca aagctaggat caagctccag taactgacga acaccgcgtc gcataagtgg
    29041 atgatcatcc acaatcatca cctgaaaagg cgttgcttca ggcatagtag tctcctgggg
    29101 ttttattaga atcattatgt ttgagcgtag cgttggtaac gctaccaggc gcgtgaatgt
    29161 taccccattt tatgtatgga catgtagtga aaactacaac cttgtcgttc gctttggcat
    29221 cattttcatt ataaagacgg aattttgcat cagctt
//
LOCUS       DAJVOY010000031        28716 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVOY010000031 DAJVOY010000000
VERSION     DAJVOY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.031"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1226
                     /gene="potE"
                     /locus_tag="OQL90_001229"
     CDS             <1..1226
                     /gene="potE"
                     /locus_tag="OQL90_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS1368079.1"
                     /translation="KLAEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKSGGMGGYA
                     EYAFGKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLSPVQIGLATIGVLWI
                     CTVANFGGARITGQISSITVWGVIIPVVGLCIIGWFWFSPTLYANSWNPHHVPFFSAV
                     GSSIAMTLWAFLGLESACANAEVVENPERNVPIAVLGGTLGAAVIYIVSTNVIAGIVP
                     NMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGY
                     FPKIFSRVTKVDAPVQGMLTIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYIL
                     SMAALVIIQKVANVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFLGWTL
                     YGLVSPCFELKNKHG"
     gene            1302..1451
                     /locus_tag="OQL90_001230"
                     /pseudo
     CDS             1302..1451
                     /locus_tag="OQL90_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            1575..2225
                     /locus_tag="OQL90_001231"
     CDS             1575..2225
                     /locus_tag="OQL90_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCS1368080.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(2266..2760)
                     /locus_tag="OQL90_001232"
     CDS             complement(2266..2760)
                     /locus_tag="OQL90_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368081.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(2975..4615)
                     /gene="pgm"
                     /locus_tag="OQL90_001233"
     CDS             complement(2975..4615)
                     /gene="pgm"
                     /locus_tag="OQL90_001233"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1368082.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMEAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(4641..5186)
                     /gene="seqA"
                     /locus_tag="OQL90_001234"
     CDS             complement(4641..5186)
                     /gene="seqA"
                     /locus_tag="OQL90_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1368083.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5371..6135
                     /gene="ybfF"
                     /locus_tag="OQL90_001235"
     CDS             5371..6135
                     /gene="ybfF"
                     /locus_tag="OQL90_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1368084.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6275..6568
                     /gene="ybfE"
                     /locus_tag="OQL90_001236"
     CDS             6275..6568
                     /gene="ybfE"
                     /locus_tag="OQL90_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1368085.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            6708..7238
                     /gene="fldA"
                     /locus_tag="OQL90_001237"
     CDS             6708..7238
                     /gene="fldA"
                     /locus_tag="OQL90_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1368086.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7448..7534
                     /gene="uof"
                     /locus_tag="OQL90_001238"
     CDS             7448..7534
                     /gene="uof"
                     /locus_tag="OQL90_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1368087.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7527..7973
                     /gene="fur"
                     /locus_tag="OQL90_001239"
     CDS             7527..7973
                     /gene="fur"
                     /locus_tag="OQL90_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1368088.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8058..8384)
                     /locus_tag="OQL90_001240"
     CDS             complement(8058..8384)
                     /locus_tag="OQL90_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368089.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFPDQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8434..9840)
                     /gene="chiP"
                     /locus_tag="OQL90_001241"
     CDS             complement(8434..9840)
                     /gene="chiP"
                     /locus_tag="OQL90_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCS1368090.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10287..11951)
                     /gene="glnS"
                     /locus_tag="OQL90_001242"
     CDS             complement(10287..11951)
                     /gene="glnS"
                     /locus_tag="OQL90_001242"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1368091.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12155..14101)
                     /gene="nagE"
                     /locus_tag="OQL90_001243"
     CDS             complement(12155..14101)
                     /gene="nagE"
                     /locus_tag="OQL90_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1368092.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14434..15234
                     /gene="nagB"
                     /locus_tag="OQL90_001244"
     CDS             14434..15234
                     /gene="nagB"
                     /locus_tag="OQL90_001244"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1368093.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15294..16442
                     /gene="nagA"
                     /locus_tag="OQL90_001245"
     CDS             15294..16442
                     /gene="nagA"
                     /locus_tag="OQL90_001245"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1368094.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16451..17671
                     /gene="nagC"
                     /locus_tag="OQL90_001246"
                     /gene_synonym="nagR"
     CDS             16451..17671
                     /gene="nagC"
                     /locus_tag="OQL90_001246"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1368095.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYIGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            17719..18471
                     /gene="nagD"
                     /locus_tag="OQL90_001247"
     CDS             17719..18471
                     /gene="nagD"
                     /locus_tag="OQL90_001247"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS1368096.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            18868..20532
                     /gene="asnB"
                     /locus_tag="OQL90_001248"
     CDS             18868..20532
                     /gene="asnB"
                     /locus_tag="OQL90_001248"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1368097.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            20913..20989
                     /locus_tag="OQL90_001249"
     tRNA            20913..20989
                     /locus_tag="OQL90_001249"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20947..20949,aa:Met,seq:cat)
     gene            20998..21082
                     /locus_tag="OQL90_001250"
     tRNA            20998..21082
                     /locus_tag="OQL90_001250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21032..21034,aa:Leu,seq:tag)
     gene            21106..21180
                     /locus_tag="OQL90_001251"
     tRNA            21106..21180
                     /locus_tag="OQL90_001251"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21138..21140,aa:Gln,seq:ttg)
     gene            21215..21289
                     /locus_tag="OQL90_001252"
     tRNA            21215..21289
                     /locus_tag="OQL90_001252"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21247..21249,aa:Gln,seq:ttg)
     gene            21305..21381
                     /locus_tag="OQL90_001253"
     tRNA            21305..21381
                     /locus_tag="OQL90_001253"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21339..21341,aa:Met,seq:cat)
     gene            21430..21504
                     /locus_tag="OQL90_001254"
     tRNA            21430..21504
                     /locus_tag="OQL90_001254"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21462..21464,aa:Gln,seq:ctg)
     gene            21542..21616
                     /locus_tag="OQL90_001255"
     tRNA            21542..21616
                     /locus_tag="OQL90_001255"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21574..21576,aa:Gln,seq:ctg)
     gene            complement(21770..22945)
                     /gene="ubiF"
                     /locus_tag="OQL90_001256"
     CDS             complement(21770..22945)
                     /gene="ubiF"
                     /locus_tag="OQL90_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1368098.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23091..24515
                     /gene="miaB"
                     /locus_tag="OQL90_001257"
     CDS             23091..24515
                     /gene="miaB"
                     /locus_tag="OQL90_001257"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1368099.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPSETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            24668..25708
                     /locus_tag="OQL90_001258"
     CDS             24668..25708
                     /locus_tag="OQL90_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1368100.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            25705..26172
                     /gene="ybeY"
                     /locus_tag="OQL90_001259"
     CDS             25705..26172
                     /gene="ybeY"
                     /locus_tag="OQL90_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1368101.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26262..27140
                     /gene="corC"
                     /locus_tag="OQL90_001260"
     CDS             26262..27140
                     /gene="corC"
                     /locus_tag="OQL90_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1368102.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27165..28703
                     /gene="lnt"
                     /locus_tag="OQL90_001261"
     CDS             27165..28703
                     /gene="lnt"
                     /locus_tag="OQL90_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1368103.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTHEVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
BASE COUNT         7006 a         7366 c         7323 g         7021 t
ORIGIN      
        1 caaagcttgc cgaagttggg acaatctcaa ttatctcctg gctggtgaca gccgtaggct
       61 caatggcact ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa tcaggcggta
      121 tgggcggtta tgcggagtat gctttcggta agtccggcaa ttttatggcg aactacacct
      181 acggtgtctc gctgctgatt gctaacgtcg cgattgctat ttcggcggtt ggttacggca
      241 ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc atcggagtgc
      301 tgtggatttg taccgtggct aactttggtg gtgcgcgcat taccgggcaa atcagtagca
      361 ttactgtgtg gggagtcatt attccggtcg tcggtctgtg cattattggc tggttctggt
      421 ttagtccgac gctgtacgct aactcatgga acccgcacca tgtgccgttc tttagcgctg
      481 taggttcttc catcgccatg acgttgtggg ctttcctcgg tctggagtca gcctgtgcca
      541 acgccgaagt agtggaaaac ccggaacgta acgtgccgat tgcagttctt ggcggtactt
      601 taggtgcggc ggtgatttat atcgtctcca ccaacgtgat tgccgggatt gtgcctaata
      661 tggagctggc aaattcaacg gcaccatttg gcctggcttt cgcacagatg ttcacaccag
      721 aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg ctgcggttca ctgcttggct
      781 ggcagttcac cattgcccag gtgtttaaat cttcatctga tgaaggctac ttccctaaaa
      841 ttttctcccg tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc attgtgatta
      901 ttcagagtgg attggcactg atgaccatta gcccgtcact gaacagccag ttcaacgtgc
      961 tggttaacct ggccgtggtg accaatatca ttccgtatat tctgtcaatg gcggcattag
     1021 tcattattca gaaggtcgct aatgtgcccc cctcaaaagc gaaagtagca aactttgttg
     1081 cctttgttgg tgcgatgtac agtttctatg cgctgtactc atccggggaa gaagccatgc
     1141 tgtacggttc tatcgtgacc ttccttggtt ggacactgta tggtctggta tcaccatgct
     1201 ttgaactgaa aaataaacac ggttaataaa acaaagtaaa aaagggcgat tgaatgatcg
     1261 ccctttctgt ttgttggcaa atagttcgtt atttatgccg catccggcag atgtagggtc
     1321 taaacgcgat cgaacacagc aactatttgc gtaattatat ggggcatctg cgacaaaaac
     1381 tggaacagga tccggcccgg cctcgccatt ttattactga aaccggtatt gggtatcggt
     1441 ttatgctttg aaaaaagtgg caatgtatgg ataaagtcat tttttattca aatataagga
     1501 acttaatatt taaaaaatgt tccatacaat tcctcatcat aaatgacacc gtagcacaca
     1561 aggagcaagt agcgatgcgt gaaatgaatc agttagtaaa tactgaagat tcagcctggc
     1621 caataatcca gaactggtta aaagacgcca caaaccatac cgaactctta ccggttaata
     1681 aaaatttagc cgaaaccgca ctttatcaat tacaggtgac aacaaaatca ccgatgggtg
     1741 cgcttgtcta tggctccgga ggtctgctga ttgataatgg ttggttacgc atagcaggat
     1801 ctggtcaccc ccggttgcca cgagatccag taagttggac acaacgccct gaattcgcag
     1861 gcgtgcgggc tttacccatc gctgacgatg tcgcaggggg aatttttgct cttaatggtg
     1921 gcgatcttgg ggaagatact ggctgcgttt attattttgc gccagatacg ctgaattggg
     1981 aaagtctgga agttggatat tctgagtttc ttcagtgggc attatccggt gatttagata
     2041 ccttctatga aaatgtacga tggcagcaat ggcgagaaga cgtaataaaa ttatcggcaa
     2101 cggaagcctt cacgttctac cctttcttat gggtacaaag tgaagaagca agaacaagaa
     2161 aagttatttc gcttactgaa ctctgggaaa tgcagtatca aatgaaagaa acattcacgc
     2221 aataagacat gttcaggtcc gggtttcatt cccggactca ctattttaat gtgtatttat
     2281 cgctttcaat tcttgctggc aattttctga ctggcagcgt cgtaactcaa taatcccgac
     2341 atgcatcatc gagccaaaaa atccgctttc gctgacataa tcctcattga ctatcggtcg
     2401 agtgccgggc ttgccctggc aaacccgcat aaaaaatgag gtgtagcatt gccaaccggg
     2461 atttaaaccg accttttctt gtgctgacat attatgaaga taagtaagtt ctttttcgtt
     2521 cccgcctgac aactgccagc ggcgggtatt atcaggtaag ttatcaccac ggcaatttgt
     2581 cttacaccgg gaaggtgcaa tatttaccgc aatgctaccc gttaatcttc tggttttatc
     2641 tttaggggtc agtaaccagt cgccagctaa tgatgattgt tcaggttggg cgcatgcagt
     2701 cagcagtaat gtggcggtaa ttagtgacac tatgacgacc agactcctgt tggtcttcat
     2761 tgggatttcc ttgagctact cagtttgttg acaaagtgtt cgttgttttt gccggatgcg
     2821 gcgtgaacgc cttatccggc ctacaaaatc tcgtaaattc aatatattgc accatcctcg
     2881 taggcctgat aagcgtagcg catcaggcaa ttctgtgttt gtcatacgta aaaaagggcg
     2941 atcttccgac cgcccttttt cttatttaat gtgtttacgc gtttttcaga acttcgctaa
     3001 caatctcaac cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc
     3061 agtagatctt atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag
     3121 tcatcacttt cagaccgccg atagaagcac cattgcccgg tgccgccgtc aggcgcgcgg
     3181 tgatcgggtc gcctgccagg gtgctggcgc tcaccatttc cggagacagc ttagacagcg
     3241 ccgctttttg tgcggaagtc gcagctgcct gcaaacggtt gtagctcggc gcaccaaagc
     3301 gttttgccag ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg
     3361 ccgccagcag acacatgatg atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac
     3421 gcaggaagga agcccctgcg ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac
     3481 catcaacaaa ccatttgaaa cctaccggga cttctaccag cttgcggccc aagtcattga
     3541 ccacacggtc gatcatcgca gaagaaacca gcgttttacc gacagcaaca tctttgcccc
     3601 actgcggacg atgctggaac aggtagttga tcgccaccgc caggtagtgg ttcggattca
     3661 tcaaacctgc cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca
     3721 gatcgaactt atcacgcagt gccagcaggc ccgccatcgc acactcggag gagcagtcca
     3781 tacggatcgc gccgtcttta tcaaggtgca taaagcggaa ggtttgatcg acctgatcgt
     3841 taacgatggt caggttgagg ttgtaatact cgccaatacg cttccagtat tcgataccgg
     3901 aaccgcccag cggatcaacg cccagcgtca ggcccgcttt ctgaatcgcc tccatatcaa
     3961 cgatatcggc cagaccttcc acaaacggct gcaccagatc ctgctctttc acatgaccgg
     4021 atgccatcgc ttcgtcgagg gagatacgct tcacgccttt caggccatcg gccagcagtg
     4081 cgttggccct gtcttccacc actttggtga cgttggtatc agccgggcca ccatttggcg
     4141 gattgtactt gataccacca tcttccggcg ggttatggga cggtgtaatc acgataccgt
     4201 ctgccagcgg accacctttt ttattgtgaa ccaggatggc attggaaacg gcaggcgttg
     4261 gagtgaagcc attgttttcc tgcacaatga catcaacgcc gttcgctgcc agcacttcca
     4321 gtacggaaat gaacgcaggc tcggacaggg cgtgagtatc tttacccaca tagcaagggc
     4381 cagtgatgcc gtttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg
     4441 gctcgttaaa gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg
     4501 cgtgctccgc attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga
     4561 cgttaatcaa atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt
     4621 tgtcctttgt ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg
     4681 aattgcatcg actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg
     4741 gtgatcaccc aatacggcgt gcctggcaca tgtttcggct tggtctgatt accatttttc
     4801 agcagcgttt gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt
     4861 gcttcggcaa acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga
     4921 ttgaccgctc gcttttgctc tgcgtattcg tcagaaagca gaagttcacg cattgcgcga
     4981 accttgtctt taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga
     5041 acctctttcg tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa catacgccgt
     5101 aaaatgtcgg atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt
     5161 tcatcatcaa cttcaatcgt tttcatctta atccagtgca gtgtcttagc tgaatacaag
     5221 tcgccaagag tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa
     5281 aactcggaat aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta
     5341 acccgacaac gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc gcaaactgca
     5401 caaaaccagc acaataattc tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac
     5461 aaccttggcg tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg
     5521 cgtaaccacg gtctttcacc gagagatccg gtaatgaatt acccggcgat ggcgcaggat
     5581 cttgttgata ctctggatgc acagcagatc gacaaagcaa catttatcgg tcactccatg
     5641 ggcggtaaag cggtaatggc acttacggca ctggcccgcg atcgcatcga taaactggtg
     5701 gcgatcgata tcgcaccggt cgactatcac gtacgccgtc atgatgagat tttcgcggct
     5761 atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc aagcagcagc aataatgcgc
     5821 cagcatctta atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag
     5881 tggcgcttta acgtgccggt attgtgggat cagtatccgc atattgtggg ttgggagaaa
     5941 atcccggcat gggatcaccc tgccctgttt atccctagcg gcaattctcc gtatgttagc
     6001 gagcagtacc gtgatgattt actggcacaa tttccacagg cacgggcgca tgtgattgca
     6061 ggcgcgggtc actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc
     6121 tatctcaatg attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg
     6181 ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcagaggc
     6241 ctggctgatt gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac
     6301 gacattagat ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta atccgctttc
     6361 gcgcgatgaa cagctgcgta ttaataaacg caaccagcta aaacgcgaca aagtacgtgg
     6421 ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc gaggcgctga acgagctggc
     6481 ggagtcgcgc aatatgagcc gtagcgaact gatcgaagag atgttaatgc agcaactggc
     6541 ggcgctgcgt agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc
     6601 ctgctcgttt gctgattccg aaagactgcc tgttctgcta tgattgcctt tatccgtggg
     6661 caattttcca cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg
     6721 gcatcttttt cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac
     6781 agcttggtaa agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg
     6841 aagcttatga cattctgctg ctgggcatcc caacctggta ttatggcgaa gcgcagtgtg
     6901 actgggatga cttcttcccg actctcgaag agattgattt caacggcaaa ctggttgcgc
     6961 tgtttggttg tggtgaccag gaagattacg ccgaatattt ctgcgacgca ttgggcacca
     7021 tccgcgacat cattgaaccg cgcggtgcaa cgatcgttgg tcactggcca actgcgggct
     7081 atcatttcga agcatcaaaa ggtctggctg atgacgacca ctttgtcggt ctggctatcg
     7141 acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt
     7201 ctgaagagtt gcatctcgac gaaattctca atgcctgatg tgatgcggcg tagactcatg
     7261 tctacgccgt attaatagat aatgccaatc aaaataattg ctacaaattt gtaacttttg
     7321 ctgttgtacc tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa atgtaagctg
     7381 tgccacgttt ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa
     7441 ttctataatg atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa
     7501 ccgcttagta acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg
     7561 gcctgaaagt aacgcttcct cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc
     7621 atcacgtcag tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc
     7681 tggctacggt atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca
     7741 attttgaagg cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga
     7801 tctgcctcga ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc
     7861 gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc
     7921 actgtgccga aggcgattgc cgcgaagatg aacatgcgca cgaaggcaaa taagccagcc
     7981 tgaatgagaa aaagccaacc tgcgggttgg cttttttatg caagggaaat aagaaaagcg
     8041 agagttacag ctctcactta tttgttatta ctgcgaattt cctgccaaac tttatcacaa
     8101 tcggctttca ccgcctgatc atttccggtt tgcgttgcac gcagacactg ctgataatcc
     8161 agtacgcgga cactttcctg gtcgggaaaa tgctgatgtt gtttctcttt cttcagcacg
     8221 tttaacacgc tctggcaggc ttcaattttt tctggcgaac cttgtgcggt gttgatacag
     8281 gcgctgtatg cctctttcag acgactgtct tcctgtggcg cggttgattg cgcacaagct
     8341 accagccccg atgccattat ggcgatgaga atcagttttt tcatagcaaa ttcctcaaac
     8401 gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac
     8461 cataaatttc acgtcacgct catcctggaa gatgttgccg taaccaccgc cccagcttgg
     8521 gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg aacatcgtgc ctttggcgcg
     8581 accgtcctga atggtgtaga ccgcatccag gctgtatgca gactcttcaa tagtccggtt
     8641 tttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat cccatgcgta
     8701 aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt catacatcgc
     8761 accgaagaag accgcttttt cgccgttagc gttgaagtcg gaacggttat cccaccagat
     8821 atccaggcgg ccgttggagg aagcgtaggc tggagtcata cgttgcagga agtatccctg
     8881 ctgaccgtca gccttaaccc aggtgccttc gaggcgcaaa tccactacgt cagccgcccg
     8941 gtaaccaaag gtcaacgctt gcagccaggc ggtgccgtca taaaggtcgt tgacgctgcg
     9001 atcgtcaact ttatcgcgcg taccatagaa ctggtagctg gtggttaacg ggctaccggc
     9061 gatatcaaat ttgtagctgg ctttggcaaa atattgatcg atataccctt ccgcctgacc
     9121 aaatgccgct tccagtacga agttattttt gaagtcgtat ttcgccccaa gggagtgcag
     9181 ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca gatgccacgg
     9241 cgctttgtat tcgttggtcc acatgtagga gaaactcaac gcaccagcat cgccgtaatc
     9301 aaaattcgcc ccggcttccg ccccctgata agtacctggc ataaagctcc agtgcggcgc
     9361 taacagcgtt tgaccagttg gctgaatgta acctgccctc gcccaaaccg gaccgtattt
     9421 aaatttggcc gcagctttat acaggcttat accgcttttg tcgccagacc agtcttcgtc
     9481 ataggcttta ttactttttg aaaacgcgat ttcgttcggg tgggagctgt cgccgttttc
     9541 cgccatttca atcgccgtaa acacagcaat atcaaggccg aacatatcag cagcataacc
     9601 ggactgaaaa tcgaggttgg cattccaggt ggagtgagaa aggttggttt tgtatttgtc
     9661 gccgtcggta acatctttac ggtcgcgttc acgctgccag taatatatac cgccggttaa
     9721 ggttgaatcg tcgatgaatc cttcggcgcg agcctccggc attgccataa agcccgacat
     9781 tgctgtaaca ccggcgatag ccagcgccag cgtactacgt ttgccactaa acgtacgcat
     9841 gggttaatcc tctttgacgt ataaattgct gcaccaaagg tgaacagcga aaaaaataaa
     9901 aattaaaaaa gtagcattga gaaaagtctc gctgactaca ggaatagact atttttcgcg
     9961 acgcgaatta tcaatctctt tctgtaacca gaatgtaaga acatgagcaa ccgcacatat
    10021 tttgatgatt tttgttacaa ggcttaagtt tgcggttgaa aggcttgcag aagaataaaa
    10081 gcgtaaattt ccagcaagat gaaagcggtt acattttatt taattatggt ttgtgatttt
    10141 aaatttattt tatggataaa ttgcgaagcg gaattaattc gcgaagaaaa gcagaaaaaa
    10201 cgccgcagag tgcggcgttt gggattgttt gctatttctt gccggatgcg acgtaaatgc
    10261 ctcacccggc ctggtagaac ttaaaattac tcgcctactt tcgcccaggt atcacgcagc
    10321 ccaacggtgc ggttaaatac cggtttttcc gctgtggaat ggcggctgtc gaggcagaag
    10381 taaccttcac gctcaaactg gaatgcttta cccgcaaccg catctttcag cgacggttca
    10441 gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa atcatccgca
    10501 gcacctgggt taggcacgct gaacagacgg tcgtacagac ggatttcaac cggcagcgca
    10561 tgtgccgcgc tcacccagtg aataacacct ttgactttac gaccatctgc cggatctttg
    10621 cttaaggtgt cggcgtcata agtacagaag atggtggtga tattgccttc ggcatctttc
    10681 tccacgcgtt ctgccttaat gacgtaagca ttacgcagac gcacttcttt acccagcacc
    10741 agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat ccaaatctca
    10801 ctgctaaacg gcacctggcg gctacccatt tccggtttgt tcggatggtt cggcatggta
    10861 accatttcgc cttcgccctg atagttttcg ataaccagtt tcaccggatc gataaccgcc
    10921 attgcgcgcg gggcgttttc gttgagatct tcacggatgc aggattccag cgacgccatc
    10981 tcaatggtgt tgtcctgcct ggtcacgccg atgcgtttgc agaactcacg aatagaagca
    11041 gcagtgtaac cacgacgacg cagaccggaa atggtcggca tacgcgggtc atcccagcct
    11101 tcaacgtgct tgtcggtcac cagcaggttc agcttacgct tggacatcac agtgtattcc
    11161 agattcaggc gcgagaattc gtactggcgc gggtgaacag gaatggtgat gttgtccagc
    11221 acccagtcgt acaggcgacg gttgtcctgg aactcaagcg tacacagaga gtgcgtaata
    11281 ccttccaggg catcgctgat gcagtgggtg aagtcgtaca tcgggtagat gcaccacttg
    11341 ttgccagtct ggtggtgttc agcaaattta atgcggtaca gcaccggatc gcgcatcacg
    11401 ataaacggtg aagccatgtc gattttcgca cgcaggcagg ctttaccttc ttcaaaacca
    11461 ccggcacgca ttttttcgaa cagcgccagg ttctcttcaa cgctgcggtc gcggtacggg
    11521 ctgtttttac ccggttgcgt cagggtgccg cggtattcgc ggatctgttc cggcgtcagt
    11581 tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg gagctgatca
    11641 aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca ctctacgtcg
    11701 tttttgatcg actcaacata ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc
    11761 aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat agatttcgca
    11821 tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc
    11881 ttaccactgg ccagatcttc atcgatgatc tgacggataa agttagtcgg gcgggcttct
    11941 gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta tgatcttata
    12001 agcggaacag gctgacaact gttagttttt taaaattgat aatctaacaa ggattatggg
    12061 gatgattgat gaaaaaaaaa agcggcagag aagatctcca ccgcttgtca ttgttggatg
    12121 cgacgctcaa gcgtcgcatc aggcataaag cagattactt tttgatttca tacagcggtg
    12181 tttgacccgc cacaacatgg ccctgagctt taatgatcaa gccactgaaa tcgtcgatat
    12241 tgctgcaaac caccgggcta atcatcgagc gggcgttagc gttcaggtaa tccagatcca
    12301 tttccagaat cggttgccct gcgcttacct gcgcaccctc ttccaccaga cgtttaaagc
    12361 ctttaccttc cagcgctacg gtgtcgatac ccatatggac gacgatctcc gcgccttttt
    12421 cggtttccag gcagaacgcg tggttggtgt tgaagatttt cacgattgtc cctgcggctg
    12481 gtgatacgac gattttatct gtcggtttca ccgccacacc gtcacccacc gctttgctgg
    12541 cgaatgcttc gtcaggaacc tgatccagtg ccacgacatc accggtaatc ggcgatacca
    12601 gctccgcgat agataccgcg tttggtacag cctgcggttt tgctacaggc gcggcagttg
    12661 acggagttgc ttcagctgac gcagcggcta ccggaccacg ggcaacgact ttcttcatcg
    12721 catcgccgat ggattctgct ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt
    12781 tcactacacc agaagcaccc agacgtttac acatcgtatc gttgacgcgg gcagagtcag
    12841 ccacggtcag gcgcagacgg gtgatacagg cgtcaattgc tttcaggttg tcagtgccgc
    12901 caaccgcagc aatatagttg gtcgccagtt gattgaggcc ttcttcagtg ttgctgttgg
    12961 cttcttcagt aacgatctca tcttctttat cttcacgacc cggcgttttc aggttgaaca
    13021 tgcggataac caaactgaac accacgaagt agatagcgaa gaagacaaca cccatcacca
    13081 gcagcatcca gacgttctgg ctggcggccg gcaggttata catcaacgcg tagtcgatag
    13141 cccccgcaga gaaagagaag ccagcatgga tacccagcag tgttgccaca aacaggctga
    13201 taccggtcag cagtgcgtgc aggaggtaca gcagcggtgc aaggaacatg aacaggaatt
    13261 ccagcggctc agtcacaccg gtcaggaacg cagtaacagc aacagaaagc agcatcccgc
    13321 caaccatcgg acgacgctct ttcggtgctg cgaagtacat cgccagcgcc gcacccggca
    13381 gaccgaacat cataatcggg aagaagccgg acatgaacat ccccgcggtg ccgtcaccgg
    13441 catagaagcg gttgatatca ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa
    13501 tctggaacca ggcgatggtg ttcagcacct gatgcagacc ggttgggatc agcagacggt
    13561 tgatgaaacc aaagataccg gaacccagcg cgcccgcaga aacgatccac tcgcctcctg
    13621 catggatagc gtgctgtacc ggcggccaga cgtaaccaaa aatggccgcc agcaccaggc
    13681 agaagaagcc ggtggcaatc ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg
    13741 gcagtttaat atcggaccaa cggttatagg ctgcgccacc aaccagaccg gtaatgatac
    13801 ccgccagtac acccatgttt atttctgggt tgatggtcac catcgctttg gttaacacaa
    13861 agtaacctac cgcacccgcc agcgccgccg cacctgcgct gtctttcgac cagctggatg
    13921 ccacaccgat ggcgaagatt aatgcgaggt tatcaaaaat cgcaccgccc gcctgggcaa
    13981 taaacgcaac gttaagtaaa tctggctgac cgaatcgcag caacagtgcc gccaccggca
    14041 gcaccgcgat agggagctgt aacgccctac cgagtcgctg gaaaaaacct aaaatattca
    14101 tcttattccc cctacgagaa ccctatttgg ctcgtttaaa gccgtatttt tattttgctg
    14161 caaattgtac tgccgatgtt ctgtaatcag attgttagat catctgctac agagtgtgtg
    14221 aaaatttaat tcgtatcgca aattaaacgc gtgtcttttg tgagttttgt caccaaatat
    14281 cgttatcatc actccctttt actggctaaa ccagaaaact tattttatca ttcaaaaaat
    14341 caggtcggat tgacgcctgt ctgcgcaaat ccaagttacg cttaaagatg cctaatccgc
    14401 caacggctta cattttactt attgaggtga ataatgagac tgatccccct gactaccgct
    14461 gaacaggtcg gcaaatgggc tgctcgccat atcgtcaatc gtatcaatgc gttcaaaccg
    14521 actgccgatc gtccgtttgt tctgggcctg ccaactggcg gcacgccgat gaccacctat
    14581 aaagcgttag tcgaaatgca taaagcaggc caggtcagct ttaagcacgt tgtcaccttc
    14641 aacatggacg aatatgtcgg tctgccgaaa gagcatccgg aaagctacta cagctttatg
    14701 caccgtaatt tcttcgatca cgttgatatt ccagcagaaa acatcaacct tctcaacggc
    14761 aacgccccgg atatcgacgc cgaatgccgc cagtatgaag aaaaaatccg ttcttacgga
    14821 aaaattcatc tgtttatggg cggtgtaggt aacgacggcc atattgcatt taacgaaccg
    14881 gcgtcttctc tggcttctcg tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg
    14941 aactctcgtt tctttgataa cgatgtcaat caggtgccaa aatatgccct gactgtcggt
    15001 gttggtacat tgctggatgc cgaagaagtg atgattctgg tgctgggtag ccagaaagca
    15061 ctggcactgc aggctgcagt tgaaggttgc gtgaaccaca tgtggaccat cagctgtctg
    15121 caactgcatc caaaagcgat catggtgtgc gatgaacctt ccaccatgga gctgaaagtt
    15181 aagactttaa gatatttcaa tgaattagaa gcagaaaata tcaaaggtct gtaattgtta
    15241 tccctgccct ggctccttgc tcagggcaat attttttaaa atcgggggtc agaatgtatg
    15301 cattaaccca gggccggatc tttaccggcc acgaatttct tgatgaccac gcggttgtta
    15361 tcgctgatgg cctgattaaa agcgtctgtc cggtagcgga actgccgcca gagatcgaac
    15421 aacgttcact gaacggggcc attctctccc ccggttttat cgatgtgcag ttaaacggct
    15481 gcggcggcgt acagtttaac gacaccgctg aagcggtcag cgtggaaacg ctggaaatca
    15541 tgcagaaagc caatgagaaa tcaggctgta ctaactatct gccgacgctt atcaccacca
    15601 gcgatgagct gatgaaacag ggcgtgcgcg ttatgcgcga gtacctggca agacatccga
    15661 atcaggcgtt aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc
    15721 ataatccgaa ttttgtgcgt aagcctgatg ccgcgctggt cgatttcctg tgtgaaaacg
    15781 ccgacgtcat taccaaagtg accctggcac cggaaatggt tcctgcggaa gtcatcagca
    15841 aactggcaaa tgccgggatt gtggtttctg ccggtcactc caacgcgacg ttgaaagaag
    15901 cgaaagccgg tttccgcgcg gggattacct ttgccaccca tctgtacaac gcgatgccgt
    15961 atattaccgg tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct gacatttatt
    16021 gcggtattat cgctgatggc ctgcatgttg attacgccaa cattcgtaac gctaaacgcc
    16081 ttaaaggcga caaactgtgt ctggttaccg acgccaccgc gccagcaggt gccaacattg
    16141 aacagttcat ttttgcgggt aaaacaatat actaccgtaa cggactttgt gtggatgaga
    16201 acggtacgtt aagcggttca tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac
    16261 attgcggtat cgcactggat gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga
    16321 ttggcgttga gaaacgtctc ggcacactcg ccgcaggtaa agtagccaac ctgaccgcat
    16381 tcacacctga ttttaaaatc accaagacca tcgttaacgg taacgaggtc gtaactcaat
    16441 aagagaaagt atgacaccag gcggacaagc tcagataggt aatgttgatc tcgtaaaaca
    16501 gcttaacagc gcggcggttt atcgcctgat tgaccagtac gggccaatct cgcggattca
    16561 gattgccgag caaagccagc ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat
    16621 cgaacgcggg ctgatcaaag aagttgatca gcaggcctcc accgggggcc gccgcgctat
    16681 ctccatcgtc accgaaaccc gcaatttcca cgcaatcggc gtacggcttg gtcgtcacga
    16741 cgccaccatc accctgtttg atctcagcag caaagtgctg gcagaagaac attacccgct
    16801 gccggaacgt acccagcaaa cgctggaaca tgccctgttg aatgccattg ctcagtttat
    16861 tgatagctac cagcgcaaac tgcgcgagct gatcgcgatt tcggtgatcc tgccagggct
    16921 tgttgacccg gacagcggca aaattcatta catgccgcat attcaggtag aaaactgggg
    16981 gctggtagaa gctctggaag aacgttttaa agtgacctgt ttcgttggtc acgatatccg
    17041 tagtctggcg ctggcggagc actacttcgg tgcaagtcag gattgcgaag actccattct
    17101 ggtgcgtgtc catcgcggaa ccggggccgg gattatctct aacgggcgca tttttattgg
    17161 ccgcaacggt aacgtcggtg aaattggcca tattcaggtc gaaccgctgg gtgaacgctg
    17221 ccactgcggc aactttggct gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg
    17281 ggtgttgaat ctgttaaagc agggctacca gagccgcgtg ccgctggacg actgcaccat
    17341 caaaactatc tgcaaagccg cgaacaaagg cgatagtctg gcgtcggaag taattgagta
    17401 tatcggtcgt catctgggta aaaccatcgc cattgctatc aacttattta atccgcaaaa
    17461 aattgttatt gccggtgaaa tcaccgaagc cgataaagtg ctgctccctg ctattgaaag
    17521 ctgcattaat acccaggcgc tgaaggcgtt tcgcactaat ctgccggtgg tacgttctga
    17581 gctggatcac cgctcggcaa tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg
    17641 tattttgctc cagcatttgc tggaaaatta atgtgctttt atagtggcgc ttattgttgt
    17701 caatattctg ggtagtccat gaccattaaa aatgtaattt gcgatatcga cggcgtgctg
    17761 atgcacgata acgtcgccgt accgggtgca gcggaatttt tgcacgggat tatggataaa
    17821 ggcctgccgc tggtgttgct gaccaactat ccttcgcaga ctgggcaaga tctggcgaac
    17881 cgctttgcca ccgcaggtgt cgatgtacct gacagcgtgt tttatacctc tgcgatggcg
    17941 actgccgatt tcctgcgtcg ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca
    18001 ctgattcatg aactgtacaa agccggtttc actattaccg atgtgaaccc tgatttcgtg
    18061 attgttggcg aaacgagttc ctacaactgg gacatgatgc ataaagcagc ctatttcgtc
    18121 gctaacggtg cacgttttat cgccaccaat ccggacaccc acgggcgcgg tttttatccc
    18181 gcgtgtggcg cgttgtgtgc aggcatcgaa aaaatctccg ggcgtaaacc gttctatgtt
    18241 ggtaaaccga gtccgtggat catccgcgcg gcattaaaca aaatgcaggc gcattcggaa
    18301 gaaacggtga ttgtcggcga taacctgcgt accgatattc tggcgggctt ccaggcaggt
    18361 ctggaaacga ttctggtgct ttctggtgtt tcatcgctcg acgatatcga cagtatgccg
    18421 ttccgcccaa gctggattta cccgtcggtc gctgaaatcg acgttatctg aatagcaacc
    18481 acgtctgcgg acgtggttgg ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt
    18541 atcaggccta cgcagctcct gcaatatatt gaattttcat gcttttgtag gccggataag
    18601 gcgttcacgc cgcatccggc atgaacaaat tgcactttgt cagcaatttg gcccccgtaa
    18661 aatacaaaca ttcaataaaa cccgctgaaa attatcgatt catcaataaa tatatcctca
    18721 caccagacct tttttcacca atcgacaatc accattacgt tttttatttt ttcaccgcca
    18781 aatcgcttgc gcacagtgct gatttgcggc atttttttaa acaagcaaac acaacaagca
    18841 acaaatacca gattaacgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa
    18901 acagacgcag ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt
    18961 ggcccggact ggtccggtat ttatgccagc gataacgcca ttctcgctca cgaacgtctg
    19021 tcaattgttg acgttaacgc gggggcgcaa cctctctaca accaacaaaa aacccacgta
    19081 ctggcggtaa acggtgaaat ctacaaccac caggcattgc gcgctgaata tggcgatcgt
    19141 tatcagttcc agacagggtc tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg
    19201 ccggaatttc tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa
    19261 gatgcctacc tgattggtcg cgaccatctg gggatcatcc cactgtatat gggctatgac
    19321 gaacacggtc agctgtatgt ggcctcagaa atgaaatcgc tggtgccagt ttgccgcacg
    19381 attaaagagt tcccggcggg gagctatttg tggagccagg acggcgaaat ccgttcttat
    19441 tatcatcgcg actggttcga ctttgatgca gtgaaagata acgtgaccga caaaaacgag
    19501 ctgcgtcagg ctctggaaga ttccgttaaa agccatctga tgtctgatgt gccttacggt
    19561 gtgctgcttt ctggtggtct ggattcctca attatttccg ctatcaccaa gaaatacgca
    19621 gcccgtcgcg tggaagatca ggaacgctct gaagcctggt ggccgcagtt acactccttt
    19681 gctgtaggtc tgccgggttc accggatctt aaagcagccc aggaagtggc aaaccatctg
    19741 ggcacggtgc atcacgaaat tcacttcact gtacaggaag gtctggatgc catccgcgac
    19801 gtgatttacc acatcgaaac ttatgatgtg accactattc gcgcttcaac accgatgtat
    19861 ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct
    19921 gatgaagtgt ttggcggtta tctttacttc cataaagcgc ccaacgctaa agaactgcat
    19981 gaagagacgg tgcgtaaact gctggccctg catatgtatg actgcgcgcg cgccaacaaa
    20041 gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat
    20101 gtggcgatgc gcattaaccc gcaggataaa atgtgcggta acggcaaaat ggaaaaacac
    20161 atcctgcgtg aatgttttga gtcatacctg cccgcaagcg tggcctggcg gcagaaagag
    20221 cagttctccg atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag
    20281 caggtttcta atcagcaact ggaaactgcc cgcttccgct tcccgtacaa cacgccaacc
    20341 tcaaaagaag cgtatctgta ccgggagatc tttgaagaac tgttcccgct tccgagcgcc
    20401 gctgagtgcg tgcctggcgg tccttccgtc gcgtgttctt ccgctaaagc gatcgaatgg
    20461 gatgaagcgt tcaagaaaat ggacgatcca tctggtcgtg cggttggtgt tcaccagtcg
    20521 gcatataagt aagaaatgcg aaacagcccc ggtgaaatcc ggggctgttt cagttattga
    20581 ggtgcttgtt gattctgata tgcagtaagg aaatgttccg ggtttgccac atcactgttt
    20641 gacaactcat cactgtaggc cagtatttca tagattaatt tcccaccact attgttgaat
    20701 tccgaatttc atcctgtacg acaaaagaat ggctgtaggg tggggaaaaa acacaaagta
    20761 aacaataatt gacgaatata gcgccacgct gttcgcaacc taaccaaata gtcactttcg
    20821 agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata atgcgccccg
    20881 caacgccgat aaggtatcgc gaaaaaaaag atggctacgt agctcagttg gttagagcac
    20941 atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccat ctttttggcg
    21001 ggagtggcga aattggtaga cgcaccagat ttaggttctg gcgccgcaag gtgtgcgagt
    21061 tcaagtctcg cctcccgcac cattcaccag aaagtgttgt acggatgggg tatcgccaag
    21121 cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt accccagcca
    21181 tcttcttcga gtaagcggtt caccgcccgg ttattggggt atcgccaagc ggtaaggcac
    21241 cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat cgaagaaaca
    21301 atctggctac gtagctcagt tggttagagc acatcactca taatgatggg gtcacaggtt
    21361 cgaatcccgt cgtagccacc aaattctgaa tgtgtcgaat acgttcggca aattcaaaaa
    21421 ccaatttgtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag
    21481 gttcgaaccc tcgtacccca gccaatttat tcaagacgct tacatcgtaa gtgcacccag
    21541 ttggggtatc gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat
    21601 cctcgtaccc cagccacatt aaaaaagctc gcttcggcga gctttttgct tttctgcgtt
    21661 cattcaatgt cgaatgcgat gttgacacgt cttatccttc aatgtcggat gcgacgctgc
    21721 cgcgtcttat ccgacctacg gttggcacgc atccggcaat gttgtaaggc tacaacccta
    21781 acgcatattt cagcgcctga cgtttcaaca cgccagcacg ctctgccgcc attaacccga
    21841 gattacgcac aaaacgcagc ggcggcagat tattactgaa tccggcataa aacagatcca
    21901 taccactttg cataatgaag ttatccgcca tgcgccgcat ctggtaacgc ttgaggacag
    21961 gataactggc ccacgcttca ccgtaactgc gggcattcac cagaacatca atcagggcat
    22021 cgacatcacg ataaccaaga ttcaccccct gccccgccag cggatggatg gtatgcgcgg
    22081 catcgcccac cagcgcaagc cccggctgca catactgcaa cgcatgtcgg cgcgtcagcg
    22141 gaaacgcacc agcggcaagc ggtgtaacgt aacccagacg cgacgggaaa tgcttcgcga
    22201 tttccgcctg gagttgcgcc atattcatat tctgcaactg gcgaatacgc gccggagagt
    22261 cataccacac cagcgatgcc cagttatcaa acaacggcag aaacgcacgc ggtccgtccg
    22321 gggtaaattg ctgccaggtg ctgtcgccgg gatcgttctc acactgtacg ctaatcaaca
    22381 tgcacgactg cgcatactgc caggcatgaa cgccaattcc cgccatctgc cgcacctgag
    22441 aatttgcgcc gtcggcacca atcaccagtt tcgcgcggat cgtttcaccg cccttcagtt
    22501 ccagctcctg aagatcatta tctcgatgta atgcaatcag cgagcctggc acacgtaacg
    22561 ttactttcgg atgcgcttcc agcgcctgcc acagcgcctg ttgcaggacg gtgttttcca
    22621 ccatatagcc aagcaacggt agcttaagtt cagcggcgtc aaacaccaca tgcgccgttt
    22681 cccactccca cgtttccagt ctgcggtaag gatggcaacg catagcctgt actgcatccc
    22741 agacccctaa ccctttaagc aatgataccg atgcggcgct gatcgccgag atccgcacgt
    22801 ccggttggct atcagcgaca aacggtgcag gttctgcgtg ctcgatcacc gttaccgaaa
    22861 atccgtgctg tgccagcccc agcgccagcg cgccgccgac cattcctccg ccgacaatgg
    22921 caatttccgt tggttgattt gtcatggtca atcatcctgt tagcaatatg ctttaagttt
    22981 acaggatttt tacccccaag ggctgataac gctcatactg gccacaacgg cagcaaagca
    23041 ttacactatg cgccctgcat tcctggctac tatttcgcaa gagcaagtcg atgaccaaaa
    23101 aactccatat taaaacctgg ggctgtcaga tgaacgagta cgattcatcg aagatggccg
    23161 atctgctgga tgccacccac ggctatcaac tgaccgacgt ggcggaagaa gcggatgtgc
    23221 tgctgctgaa cacctgctca atccgcgaga aggctcagga aaaagtcttc catcagttgg
    23281 gtcgctggaa actgttaaaa gagaagaatc cagacctgat tatcggcgtc ggtggctgcg
    23341 tggcatcgca agaaggcgag cacattcgcc agcgcgccca ctatgtcgat attatttttg
    23401 ggccgcaaac gctgcaccgt ctgccggaga tgatcaactc cgtgcgcggc gaccgcagcc
    23461 cggttgtaga tatcagcttc ccggaaatcg agaagtttga ccgtctaccg gaaccgcgcg
    23521 ccgaagggcc gaccgcgttt gtctccatca tggaaggctg caataaatat tgcacctact
    23581 gcgtggtgcc ttacacccgt ggtgaagagg taagccgtcc gtctgacgat attctgtttg
    23641 agattgccca gcttgcggct cagggcgtgc gtgaagtcaa cctgctcggt cagaacgtga
    23701 acgcctggcg tggtgaaaac tacgacggct ccaccggatc gtttgccgat ctgctgcgtc
    23761 tggttgctgc gatcgacggg atcgatcgta ttcgctttac caccagccat ccgatcgaat
    23821 tcaccgacga tatcatcgaa gtgtatcgcg acacgccgga gctggtgagc ttcctgcatc
    23881 tgccggtaca gagcggttcc gatcgcattc tgaacctgat gggccgtact catacggcac
    23941 tggagtacaa agcgatcatc cgtaaactgc gtgcggcgcg tccagatatt cagatcagtt
    24001 ctgatttcat cgttggcttc cctagcgaaa ccaccgaaga cttcgagaaa acgatgaagc
    24061 tgattgccga cgtcaatttc gacatgagct acagctttat cttctctgca cgtccgggta
    24121 caccagccgc cgatatggtt gatgatgttc cggaagaaga gaagaagcag cgtctgtata
    24181 ttctgcaaga acgtatcaat cagcaagcga tggcatggag ccgccggatg ctcggcacca
    24241 cccagcgtat tctggtagaa gggacttcgc gtaagagcat catggagctt tccgggcgta
    24301 ccgaaaataa ccgcgtggtc aacttcgagg gcacaccgga tatgatcggt aaattcgtcg
    24361 atgtagaaat taccgacgtc tacccgaact ctctgcgcgg taaagtggtg cgtactgaag
    24421 atgaaatggg cctgcgcgtg gcagaaacac cggaatcggt gattgcccgt acccgcaaag
    24481 aaaacgacct tggcgtgggt tattatcagc cgtaattctc aggcccgccg ttccggtggg
    24541 ccttgttttt tctctcttta tccccatctt tatcgcaatg cttgcgcctt tttcctgtgg
    24601 cgtgaataat ttccttatag gccggtgaat gttcagcttc cggcggcata cagataagag
    24661 gaacggtttg aacatagaca ctcgcgaaat caccctggag ccagcagaca acgcgcgtct
    24721 gttgagcctg tgcggcccgt ttgatgacaa catcaagcag ctcgaacgcc gtctcggcat
    24781 cgagatcaat cgccgcgaca accactttaa actgaccggc cgtccgattt gcgtcaccgc
    24841 tgcggcagac attctgcgta gcctgtatgt cgatacagcc ccgatgcgcg gtcagattca
    24901 ggatatcgaa ccggaacaga tccaccttac gattaaagaa gcgcgagtac tggagcaaag
    24961 tgcggagagc gtgccggagt acggcaaagc ggtcaatatc aaaaccaaac gcggcgtaat
    25021 taagccgcgc acgccaaacc aggcgcagta catcgccaat attctcgacc atgacattac
    25081 cttcggcgtt ggcccggcgg gtacgggtaa aacctacctg gcagtggctg cggcagttga
    25141 tgccctggag cgtcaggaga ttcgccgtat tctgctgact cgtccggcgg tcgaagccgg
    25201 tgagaaactg ggcttcctgc ctggcgattt aagccagaaa gtagacccgt atctgcgccc
    25261 actgtacgac gcgctgtttg aaatgctggg ctttgagaaa gttgagaaac tgattgagcg
    25321 caacgttatt gaagtcgcgc cgctggccta tatgcgtggt cgtacgctga acgacgcgtt
    25381 tatcattctc gatgagagcc agaacaccac catcgaacag atgaagatgt tcctgacccg
    25441 tatcggtttt aactcaaaag cggttatcac cggcgacgtc acacagatcg acctgccgcg
    25501 taatactaaa tcaggcttac gtcacgccat cgaagtactg gccgatgtcg aagagatcag
    25561 ctttaacttc ttccacagcg aagacgtggt tcgtcaccct gtggtggcgc gtatcgttaa
    25621 cgcctatgaa gcctgggaag aagccgaaca aaaacgaaaa gcggcgctgg cggcagaacg
    25681 caagcgcgaa gaacaggaac aaaaatgagt caggtgatcc tcgatttaca actggcatgt
    25741 gaagataatt ccgggttacc ggaagagagc cagtttcaaa catggctgaa tgcggtgatc
    25801 ccgcagtttc aggaagaatc ggaagtgact attcgcgtgg tcgataccgc cgaaagccac
    25861 agtctgaatc tgacctatcg cggtaaggat aagccgacca acgtgctctc cttcccgttt
    25921 gaagtgccgc ctggcatgga aatgtcgcta ctgggcgatc tggttatctg ccgtcaggtg
    25981 gttgagaagg aagctcagga gcaaggcaaa ccactggagg cgcactgggc gcatatggtg
    26041 gtgcacggca gtctgcattt gttaggttac gatcacatcg aagatgacga agcagaagaa
    26101 atggaagccc tcgaaacaga gattatgctt gctctgggct atgaggatcc gtacattgcc
    26161 gagaaagaat aatttgccag gcatttgact ggcagacgtc cccgccgttg gtgattaacg
    26221 acggcgtttt gattaactaa catgagaacc catacgacgc catgagcgac gacaattcac
    26281 acagtagtga cactataagc aacaagaagg gatttttctc cctgttactc agccaacttt
    26341 tccacggtga accgaaaaac cgtgacgaac tgctggcgct gatccgtgat tccgggcaga
    26401 acgaccttat cgacgaagat acgcgcgata tgctcgaagg ggtgatggac atcgcagacc
    26461 aacgcgttcg cgacatcatg atcccccgct cccagatgat taccctgaaa cgcaaccaga
    26521 cgctggacga atgccttgat gtcatcatcg agtccgccca ctcacgtttc ccggtgatta
    26581 gcgaagacaa agatcacatt gaagggattc tgatggcgaa agacttgctg ccgtttatgc
    26641 gcagcgatgc tgaagccttc agcatggaca aagtgttacg tcaggcggtt gtcgttcctg
    26701 aaagtaagcg cgtagaccgg atgctgaaag agtttcgctc tcagcgttac cacatggcga
    26761 tcgtcattga cgaattcggt ggggtttccg gtctggtgac cattgaagac atcctggaac
    26821 tgattgttgg tgagattgaa gacgaatatg acgaagaaga tgatatcgac ttccgtcagc
    26881 tgagtcgtca tacctggacc gtgcgcgcac tggcttccat tgaagacttc aacgaagcgt
    26941 tcggcaccca ctttagcgat gaagaagtcg acactatcgg tggtctggtg atgcaggcgt
    27001 ttgggcatct tccggcgcgt ggcgaaacta tcgacatcga cggttaccag ttcaaagtgg
    27061 cgatggccga cagtcgacgt attattcagg ttcatgtcaa aatcccggat gactcacccc
    27121 agccgaagct ggatgaataa aaccgaaact ggatagataa ctacatggct tttgcctcat
    27181 taattgaacg ccagcgcatt cgcctgctgc tggcgttatt attcggtgcc tgcggaacgc
    27241 tggccttctc tccttacgac gtctggcctg cggcgattat ttcgttgatg gggcttcagg
    27301 cgttgacctt taaccgccgt ccactccagt ctgccgctat tggcttttgc tggggatttg
    27361 gcctctttgg cagcggtatt aactgggtct atgtcagcat cgcgaccttt ggcggaatgc
    27421 ctggcccggt taacatcttc ctggtggtgc tgctggcggc gtatttgtcg ctgtataccg
    27481 gactgtttgc tggcgtgctg tcgcgtctgt ggccgaaaac cacctggctg cgcgtggcga
    27541 ttgccgcccc tgctctctgg caagtgaccg agtttctgcg cggttgggta ctgacaggct
    27601 tcccgtggtt acagttcggc tatagccaga ttgatggccc gttaaaaggg ctggcaccga
    27661 taatgggcgt ggaagccatt aacttcctgc tgatgatggt tagtggcctg ctggcactgg
    27721 cgttggtcaa acgcaactgg cgtccgctgg tggtggccgt cgtgctgttt gccctaccct
    27781 tcccgctgcg ttacatccaa tggtttaccc cgcaaccgga gaaaaccatt caggtttcga
    27841 tggttcaggg cgatattccg caatcactga aatgggatgg agatcagctt cttaatacgc
    27901 tgaagattta ctacaacgca acggctccgc tgatgggcaa atcatcgttg attatctggc
    27961 cggagtcggc gataaccgat ctggaaatta atcagcaacc gttcctcaaa gcactggacg
    28021 gtgagttgcg cgataaaggt agctcgctgg taaccgggat tgtcgacgcg cgtctcaata
    28081 agcagaaccg ctacgatacc tacaacacca tcatcacact gggtaaaggt gcaccgtaca
    28141 gctacgaatc agccgatcgc tataacaaaa accatctggt gccgtttggc gagtttgtcc
    28201 cgctggagtc gattctgcgt ccgttagcac cgttctttga tctgccgatg tcgtcgttca
    28261 gccgtgggcc atatatccag ccgccgctgt cagtaaatgg tattgagctt actgcggcta
    28321 tttgctacga gatcattctc ggcgagcaag tgcgcgataa cttccgcccg gataccgact
    28381 atctgctgac tatctccaac gatgcgtggt ttggtaaatc tattggtcca tggcaacact
    28441 tccagatggc gcgaatgcgt gcgctggagc tggcgcgccc actgttgcgc agcaccaaca
    28501 acggcattac ggcggtgatt ggcccgcagg gtgagattca ggcgatgatc ccgcagttca
    28561 cccacgaggt gttaaccact aatgtgacac cgaccaccgg actcacaccg tatgcacgta
    28621 ccggcaactg gccactgtgg gtgctgacgg cactgtttgg ttttgccgct gtgttgatga
    28681 gtctgcgagc acgtaaacgt tgatgtgcct gatgta
//
LOCUS       DAJVOY010000032        28457 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVOY010000032 DAJVOY010000000
VERSION     DAJVOY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.032"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..193)
                     /locus_tag="OQL90_001262"
     CDS             complement(<1..193)
                     /locus_tag="OQL90_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCS1368104.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSSEGI"
     gene            complement(329..3448)
                     /locus_tag="OQL90_001263"
     CDS             complement(329..3448)
                     /locus_tag="OQL90_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1368105.1"
                     /translation="MIGQFLSATEILAKNYVRNKMVKNPFYSNLKWNFIEKNIIRLTS
                     SPVKSVLCISAFSFVLLYVGYLNELFIKNNLLHYFPFRHSLTEWQTTILSGQLTIIGI
                     VYPLVIGLVSVLFQKKADRKIAQTAYQRYSGFMLAGLSGLFLSGFILLSVLIKTVFGS
                     YLYGIACLISILWLLINIVLSIWFFIVSLEILDDVKRQIIIKRYIAFEIVMPHICNKI
                     SAKLRLYPIYQKHNYSNLEITQADYKGEYISVASSYSKEDELSLYHRPFQLTLNLINY
                     QLKKKNHFASFVIGDNRTKETESTGKILFSVKNIKPDSLLIKILKQCFYRAPIKGGDF
                     SVSLTMQAITADTYMYLRDSDLISFDNAISALINNFNNLCDLYFFQDDNTNNNFLLIT
                     TELFERSFQYEFSDEVYKISNNSMDKINLSERFFELCLWSGVRIINNRKHLISNELCI
                     YMGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQYQESINFRFCNTEN
                     SDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSMNIDSHSVEKYSYK
                     GQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSRINTSDKLMTHYIK
                     LILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRD
                     YDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSNKNDSVSKEIKEAIENNYITYRE
                     KQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDA
                     AIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYA
                     EGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADL
                     AGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQP
                     FSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKI
                     AHSQRVTSE"
     gene            complement(3697..4806)
                     /locus_tag="OQL90_001264"
                     /pseudo
     CDS             complement(3697..4806)
                     /locus_tag="OQL90_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            4964..6148
                     /locus_tag="OQL90_001265"
     CDS             4964..6148
                     /locus_tag="OQL90_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCS1368106.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6148..6658
                     /locus_tag="OQL90_001266"
                     /pseudo
     CDS             6148..6658
                     /locus_tag="OQL90_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            6714..7088
                     /locus_tag="OQL90_001267"
     CDS             6714..7088
                     /locus_tag="OQL90_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1368107.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            7097..7201
                     /locus_tag="OQL90_001268"
     CDS             7097..7201
                     /locus_tag="OQL90_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCS1368108.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            7239..10046
                     /locus_tag="OQL90_001269"
     CDS             7239..10046
                     /locus_tag="OQL90_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1368109.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            10059..10547
                     /locus_tag="OQL90_001270"
     CDS             10059..10547
                     /locus_tag="OQL90_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1368110.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(10576..11174)
                     /locus_tag="OQL90_001271"
                     /pseudo
     CDS             complement(10576..11174)
                     /locus_tag="OQL90_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            11953..12486
                     /locus_tag="OQL90_001272"
     CDS             11953..12486
                     /locus_tag="OQL90_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1368111.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(12515..13078)
                     /locus_tag="OQL90_001273"
     CDS             complement(12515..13078)
                     /locus_tag="OQL90_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1368112.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(13047..15197)
                     /locus_tag="OQL90_001274"
     CDS             complement(13047..15197)
                     /locus_tag="OQL90_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCS1368113.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAVQKNGDTLSGGL
                     TFENDSILAWIRNTDWAKIGFKNDADGDTDSYMWFETGDNGNEYFKWRSRQSTTTKDL
                     MTLKWDALNILVNAVINGSLGVGSTNALGGSSIVLGDNDTGFKQNGDGILDVYANSQR
                     VFRFQNGVAIAFKNIQAGDGKKFTLSSSNNSTKNATFNLWGASTRPVVAELGNEAGWH
                     FYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYVKDVRLGTRVVQLMARGGRYEKAGHAI
                     TGLRIIGEVDGDDEAIFRPIQKYINGTWYNVAQV"
     gene            complement(15200..15730)
                     /locus_tag="OQL90_001275"
     CDS             complement(15200..15730)
                     /locus_tag="OQL90_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCS1368114.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(15723..16619)
                     /locus_tag="OQL90_001276"
     CDS             complement(15723..16619)
                     /locus_tag="OQL90_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1368115.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCASFTISEGGRNE"
     gene            complement(16623..16952)
                     /locus_tag="OQL90_001277"
     CDS             complement(16623..16952)
                     /locus_tag="OQL90_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCS1368116.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            complement(16970..17536)
                     /locus_tag="OQL90_001278"
     CDS             complement(16970..17536)
                     /locus_tag="OQL90_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCS1368117.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTSRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(17548..18183)
                     /locus_tag="OQL90_001279"
     CDS             complement(17548..18183)
                     /locus_tag="OQL90_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCS1368118.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(18176..18643)
                     /locus_tag="OQL90_001280"
     CDS             complement(18176..18643)
                     /locus_tag="OQL90_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1368119.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(18781..19188)
                     /locus_tag="OQL90_001281"
     CDS             complement(18781..19188)
                     /locus_tag="OQL90_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCS1368120.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(19185..19577)
                     /locus_tag="OQL90_001282"
     CDS             complement(19185..19577)
                     /locus_tag="OQL90_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCS1368121.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(19574..19897)
                     /locus_tag="OQL90_001283"
     CDS             complement(19574..19897)
                     /locus_tag="OQL90_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1368122.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(19900..20100)
                     /locus_tag="OQL90_001284"
     CDS             complement(19900..20100)
                     /locus_tag="OQL90_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCS1368123.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(20100..20594)
                     /locus_tag="OQL90_001285"
     CDS             complement(20100..20594)
                     /locus_tag="OQL90_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCS1368124.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(20762..21508)
                     /locus_tag="OQL90_001286"
     CDS             complement(20762..21508)
                     /locus_tag="OQL90_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCS1368125.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKKQQK"
     gene            complement(21554..22426)
                     /locus_tag="OQL90_001287"
     CDS             complement(21554..22426)
                     /locus_tag="OQL90_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCS1368126.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(22423..22605)
                     /locus_tag="OQL90_001288"
     CDS             complement(22423..22605)
                     /locus_tag="OQL90_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCS1368127.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(22629..23465)
                     /locus_tag="OQL90_001289"
                     /pseudo
     CDS             complement(22629..23465)
                     /locus_tag="OQL90_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
     gene            23620..25371
                     /locus_tag="OQL90_001290"
     CDS             23620..25371
                     /locus_tag="OQL90_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCS1368128.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            25371..26407
                     /locus_tag="OQL90_001291"
                     /pseudo
     CDS             25371..26407
                     /locus_tag="OQL90_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(26886..27476)
                     /locus_tag="OQL90_001292"
     CDS             complement(26886..27476)
                     /locus_tag="OQL90_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCS1368129.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADKHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(27540..27851)
                     /locus_tag="OQL90_001293"
     CDS             complement(27540..27851)
                     /locus_tag="OQL90_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCS1368130.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(27856..>28457)
                     /locus_tag="OQL90_001294"
     CDS             complement(27856..>28457)
                     /locus_tag="OQL90_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCS1368131.1"
                     /translation="KKANVMRPVEYQNGEAFTIRNVQVMPESIPAGFKALADMSPFES
                     LLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSHHIAN
                     TIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVMVVGG
                     GAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
BASE COUNT         6529 a         7161 c         7398 g         7369 t
ORIGIN      
        1 ggatgccctc tgatgagtga atgggggagg cgtgctttga agtgaatttg tggaaggctt
       61 ccagtgttga gaaacatacg ccgcattcta gattgttaca ctgataatac ttttgtctca
      121 cggtgtttga atcattttcc ggacgactgg tgcggatacg ggcagatgcg ccacaaagcg
      181 gacaacggaa catagcgacc tctcttattg tagtgcttac actattctag gttgtattct
      241 ctaagattta aacatgactt tctctatata atgattttta gcattagtta ataaacgaac
      301 ggcactctaa ggagcgccgt caaagatatt attctgatgt cactctttgc gagtgagcta
      361 tttttaaatc agcgtttcct tcaaatacta tattcaactc atagttaaga gttagcttga
      421 atttatctga gtcacttttg atattctctg aagtgacatt tactaactta ttattatcat
      481 attctctaat aattaattta gtgaagtagt tatcctttac cacaagagaa aatggttgat
      541 taacaaatgg taagaagtaa accttacggg taccggattc agtagttatg ttcatagagc
      601 catcatcatt ttttctcatt agttctctga ggttgggatt atcgaaaagc atacgattat
      661 attgctgaaa gatctcttta ctcatcacaa gaaccagttt gccagccaag tcagcagatg
      721 attttatgtg atcaatcaac atattgaaat cttctattac ggtatgttgc gaacagccag
      781 tgctaaataa atttgaaagt attattctct ggatgtcctt gagaattaag tctgcttgaa
      841 tatcaccttc attgatataa gggatggatt ctattccttc ggcatacggt tctttatcta
      901 tcttaataga tatcttatgt tttgtgaaac aatcgctagc ctgcttaaaa tcaatagatt
      961 tgaaaagtga aagagggagc gttttcttga tgcgtttacg aagatgttct gatgttttac
     1021 gtgcagtgct atctagtcga tgtacatcta tggcagcatc ctgaagtctc atatcaagat
     1081 ctttctttag catgttaata tgctcttgaa gcaactttct taagttttct ttttttgttt
     1141 ggagatcgtc caatgttaaa gtcagcttga ttttagtgta ttcgatggag ttacaaattt
     1201 ttgataatac tccaatgagt cgttgcttct cacgatatgt tatatagttg ttttctattg
     1261 cttcttttat ttcttttgat acgctatcgt tcttgttaga aaaaattaat aatagctgaa
     1321 cccatgattg ttccatatca ataaatccac agtcaaaaac attactgtaa actctgccca
     1381 tcaccatgtc tgttttatca tagtcacgta aacgccttgc taaactattc atccacccat
     1441 tgtgctccat attttcagac catgtccaca ggcagattct aattaatatc ttgattagtt
     1501 ggcttgcatt atcaatttct tcagtgggag tttcatatcc accggtttgg tcaataagtt
     1561 taccctctaa aataagttta atgtaatgtg tcattagttt gtcagaagta ttgattcttg
     1621 atgtcagata aagacatgtg catatacgca tgtcagtcaa cgcatttatg atggcaatat
     1681 tgaaccactc tctgtctgaa ttgaagttta gttttttgga tatgaaaaaa cccgggttaa
     1741 aaagctggcc tttgtaactg tatttttcta ccgaatggct gtcaatattc attgagtgct
     1801 gccatcggtt aataagatcg acggctgtat caatagtaga aggatcgcga gtctgtgttg
     1861 catcaataat catcgatgga agttcttgta gttgcgtttt gcaaaataat tcaaataaat
     1921 ctgaattctc tgtattgcag aaacgaaagt ttatgctttc ttgatattgc tcccaaacag
     1981 tgatataggt tcttaatata cgattataac gtgaacgaag cgaggcattt aagagtgatt
     2041 ggttgtttcg gaaccattct gttaatattg accattgtga acgagtaatt cccatataga
     2101 tacaaagttc attgctgata agatgttttc tattattgat aatacgcact ccgctccaaa
     2161 gacataattc aaaaaatctt tcagaaagat taattttatc catggaatta tttgatattt
     2221 tataaacttc atctgaaaac tcatattgaa aactccgttc aaataattct gtagtgatca
     2281 gtaagaagtt attgttggta ttatcatctt gaaaaaaata caaatcgcag agattattaa
     2341 agttattaat taatgcagag atagcattgt caaaactaat gagatcagaa tcccgcagat
     2401 acatatacgt atctgctgtt atagcctgca ttgtaagact tacggaaaaa tctcctcctt
     2461 taatcggagc cctataaaaa cattgcttaa gtatttttat taataagctg tctggtttga
     2521 tatttttaac gctaaaaagt attttacccg tggattctgt ttcttttgta cgattatcac
     2581 caatgacaaa tgacgcaaaa tgattcttct tttttagttg gtaattaata agattaagag
     2641 taagttgaaa aggacggtga tataaactta actcatcttc cttagaataa ctgctagcca
     2701 cagatatgta ttctcctttg taatcggctt gcgtgatttc taaatttgaa tagttatgtt
     2761 tttgatagat agggtataac ctaagcttgg ctgatatttt gttacaaata tggggcatta
     2821 ctatttcgaa ggcaatgtat cgctttataa ttatttgccg ttttacatca tcaagaattt
     2881 caagactcac gataaagaac caaatagaaa gaactatgtt tatcaaaagc cataatatgc
     2941 tgattagaca agctatacca taaagataac ttccaaagac agttttaatt agtacactga
     3001 gaagtataaa tcctgataag aaaagcccgc tgagtccagc aagcatgaat cctgagtaac
     3061 gctgataagc agtttgggct atttttctat ctgccttttt ttggaataag acacttacta
     3121 aacctataac aagggggtat acaataccaa taattgtcaa ttggccactt aaaatggttg
     3181 tttgccattc ggttaatgaa tgcctaaacg ggaaataatg taaaagatta ttttttataa
     3241 ataattcgtt taaatatcca acataaagta gtacaaatga aaaagctgat atgcaaagta
     3301 ctgatttaac cggtgaggat gttaatctta tgatgttttt ttcaataaaa ttccatttta
     3361 aattcgaata aaatggattt ttaaccattt tattacgaac atagttcttt gctaaaatct
     3421 ctgttgcact taaaaattgt ccaatcatga attactcttc tataaagtgt gggtctacta
     3481 actaaactgg aacttttact attgagtcat attcccttgg acgggatgga ctgtgaagat
     3541 tatacttgac ttcctctgtc atgtgggaaa aacatccgta ttattgttag catctggttg
     3601 atgccccaac ctctgacatc tagtgggctg atacattatc ttttgttctt actgtcagtg
     3661 tagaagcaca tctcaaaaat aatattttta agagatttat tctgtttctg ctatccattc
     3721 cgggattttt gcttcaagtt caagctgcgt ggtaaagccg ctgttatcaa tggtgtgctc
     3781 ggcttttgca atagtccagt cctgataatc aatctcgctt ttaaagcctg tcactgtgcc
     3841 atgcatttcg ggatagagtt ctgcacgacc acgcgccagc gtgatggaga atgatgcggc
     3901 tccgcgttgt agctgctgcc actttgccgc cgctgcgcgt cttgctgcct gctcgttctg
     3961 ataagtcttg cgtaacacaa acacgttgcc ttccgcacct tccatatagt cgccttcccg
     4021 actgctgctt ttctcctttt tgggttttgg cggtttgcgg cgtttcacgc tgactttttt
     4081 ctttttcccg taattaagat caagccagta ggcgcgtacc cccgtatacg cctcgcggtc
     4141 agcaatgcgg aactgatggc gatcgccgct gctgcgtgta atggcgaacg agggcaacgg
     4201 ctgcccctgt gcgttcacgc caccacctgg catgatgaat aacagattgc cgctttttac
     4261 cgtggcgatt gctcccagca tttccgccat gcgcgtaaga aaggacatgt cgctttcttc
     4321 ggtctggtcg gcgtggtcga tttcgatatc catcagcatt tcgctgattt gcggtttcag
     4381 accgtaccga tgagtgatgg cggataccac acgctcaacg gtcacatcat gccaggacac
     4441 ctcacgttta acgttaaatt catcccgaaa atctgcgctt ctggctgaaa cagtcagcct
     4501 gtccggcggt ccttcgtgag cgatttcatc aacaatgtaa gtgccttttt ctgtcagcgg
     4561 ttcccctttc cagccaatga gaaccgtcag acgcgcgccc cgtggcggta gctgcaactg
     4621 accatccgca tcatccagcg tgatggtgag ctggtccgcc tcaaatcccc ggttgtcggt
     4681 cagttacagg ctcatcaggc gctccgccac gcctgacagc gttttaccct ccgagagaat
     4741 atcaaaatcc ggcattttca cgggggccgt gccctgactg agcaattgca tggtggtgtc
     4801 ggtcatctgc tccctccctg tgcggcatgg tcgcatgtgc gtgcggaggg ggttactgct
     4861 ttttgttgtc gccgtggcgg gagaacggcg caggggtgag attacgcgcg tggtgggtga
     4921 tgattgttgc tgaatcattt aacggataca aggggctgaa gctatgagtg aaactcgttt
     4981 tcatggtgcc cgtgttacgg agaataccga cctggtaaca gcgattaacg atgttgattc
     5041 cagcgttatc ggtatcgtgg caacggcgga tgatgcggac gcgaagctgt tcccgctgaa
     5101 caagcccaca ctgctgaccc gcgtcaatga cgtgctggga aaatgcggga caacggggac
     5161 gctttatcgt gcgcttaagg ccatcgcaga ccaggtgagc acaaaggtga tcgtcgttcg
     5221 cgtggctgaa cacaaagaag aagacggaaa gacgcaggat caactggtta tcggtggttc
     5281 tgaggatgac ggcagctata cggggatgta tgcgctgctt gttgcagagc aggatgaaag
     5341 catcggatac cgtccgcgta ttctggccgc gccggagctg gacacggagg cggtaacaaa
     5401 atccctgtgc gtgattgcag ataaactgcg cgcgtttgtg tatgcctcat gtcatggctg
     5461 taacacgatg gccgaggcaa ttacctaccg ccagaaattc aacgaacgtg aggtgatgct
     5521 cttatggccg gacttcatcg cctacaaccc gaaaaatggc aaaaacgaaa cgttccccgc
     5581 gcctgcctat gcgtgcggcc ttcgtgcgta cattgaccat gaacagggat ggcacaaatc
     5641 gctgtccaac gttccggtta aaaatgtgct ggggatgtcg aggcatgtgt tctggtcgtt
     5701 gcaggccgaa gacagcgatg ccaacagcct caacaacaaa gaaatcacga ccattattcg
     5761 tcgcaacggg ttccgcttct ggggtaaccg cacaccggaa acgaacgcct acatctttga
     5821 ggtgtatacc cgaaccgcac aggtgctggc tgattcaatt gcggaagcgc agtttgaaac
     5881 catcgacagt ccactgacgc ctgcgaatgt gaaggatgtt atcagtgcca tcagggcaaa
     5941 actggattca ctggtgactg ccgggaaact gattggcgcg gagtgctggt atgacgtggt
     6001 ggataacagc accacggatt tacgtcaggg acgtgtgcgt attcgctaca aatatacgcc
     6061 cgttccgcca ctggaagaca tggagcttta ccagacgttt actgatgaat actttgaacc
     6121 cgcatttgcg gtgctgggag gtgcctgatg gctgtgccaa aacatcttcg cttttttacg
     6181 ctgtttgtgg atggtgaaaa cgaagtgggt aatgtgacgt ccgtcactct gcctaagctg
     6241 acgcgcaaaa ccgacagcta ccggggtggt ggcatgatgg gtgcggtaag tattgatctc
     6301 ggtctggacg actccgcgct tgatgcgagc tttgtcatgg gggcgcagtt cgtgagctgt
     6361 tccttaagta tggcggcacg attgacggca cgctgctgcg ttttgcgggt gaatactaca
     6421 ccgatgcaga aagcgacctg tatgaagtcg aaatgcgcgg acgtgtgacg gaaattgata
     6481 tgggggaagc caaacagggc gaagccacat cacacactta cgccattaaa acacctacta
     6541 caagctgagt gttaacgatc gcccgttgtg ggagattgac ctgctgaact tcatttaccg
     6601 gaaggacggc aaggacattg tgcccgatcg catccgttcc gcgcttgggc ttggctgata
     6661 agtaatatgc tggcggcgca gtgcgtcgcc tctgactgaa aaggagacaa cagatgaaag
     6721 acatcgatac tgaaacccgg aataacaccg tggcggataa tgtgacggca gttgaggata
     6781 tggctgtcga acgtggcgta aaactgaccc gaccaattga gcgtggtggc gaaaaaatca
     6841 cgtatgtgga gatcaccggg gctattgagc aggctggatc cctgcgtggt ctgtcgctgt
     6901 ctgatgtgct gaatctgaaa gcggatacca tgttcacgct gttgcctcgc gtgacctcgc
     6961 cacgactgga tgaagtgatg attaaaaaaa tgtcgtcacg cgattttatt cagttgtgcg
     7021 ctgtggctgt aaattttatg agcgagccag actctggcgc gaagagcgtg caggagacgg
     7081 cagcgtaatc accctggtgt gctttgagca catcgaagat ctggtggcgg atattgccgc
     7141 catttttaac tggtcgcccg ccgaaatctt catgatgacg cccggcgaag tggttagctg
     7201 acgtgagcgg gcggcacttc gtagcgggaa tgcagacaat gaagactctt gatatccggg
     7261 tcgctttcag cgccgttgac aggctgaccc ggcctgccga aaacgcccgc cgcctgatgg
     7321 ggcagtttgg tgactccatc cagcgaacgc agggggcgat caaaaatctc gagcgtcagg
     7381 cgcgatcatt tgagcgcgcc cgtgacgctg tcagtaaagc ggatgctggc atcgtgaaag
     7441 cacgacgcca gcttaacgcc cttaatcagt tacaacgcac gggtacagtg ctcagcgaaa
     7501 aacaacaaaa gctgatgcag cagttaagca cccggcttga acgcctgaat gaatcgcgca
     7561 cacgggaaat tcagaaaatg cgggagcttg gcggagagct gaaacgccac ggcatttccc
     7621 tgacaggcag cgataacacc atccagcagg ccatcagacg caccgaacag tacaacaacc
     7681 agcttgaacg cgaacggcag gcgcttgcgc gtgtaacacg ggcgcgtgag cggtattcgc
     7741 gcgcgcagga aacagcggga aaactgaaaa caggtggtgc gctggcaatt ggtgcggcag
     7801 cggcgggcgg ctatgctgcc gggcgttttt tgcagcctgc gatcgggttc ggcaaagaga
     7861 tgtcccgcgt tcaggcactg acgcgaatcg accagaacag cccgcagttt aaggcgctgc
     7921 gtgagcaggc gttaaaactt ggctctgaaa cgcagttcac cgcaggcgat gccgccagtg
     7981 gacaggcatt tcttgcaatg gctggcttta caccacaggc cattcaggct gcgctccctg
     8041 gcgtactgag catggcaacg gctggcggta tggacctcgg tgagacggcg gatattggct
     8101 caaatattct gacgcagttc ggtctttctg ctgaccagat ggaccgggtc ggcgacacac
     8161 tcaccgcagc gtttacccgt accaacactg accttcgcgc actgggtgaa accatgaaat
     8221 atgcaggtcc ggtggcgggt aagctgggaa tatctctgga gcaggccgca gcgatggcgg
     8281 gcgtgctggc gaatatgggt atcaggggga gtgatgctgg tacggcaatg cgtgccagcc
     8341 tggctcgtct ggcatcaccg ccaaaggcgg cagcagaggc gctgaaagag cttggcgtgt
     8401 ctgtctcgga tgccgggggc aaaatgcgcc cgatggagga tgtgctggct gacctttata
     8461 aagctacccg caaatacggg gaagttgacc gggtatcgtt ctttaaggac attgccggag
     8521 aagaggcttt cacatcattt atggccctcg ttgatgcggc aggtgacggc tccttaccca
     8581 aactgagaaa agaacttgaa ggcgcgcgcg gtgaggctga acgcacggca aaggttatgg
     8641 ccaacaacct tgacggcgat ctgaaatcac tcagcagtgc atgggaaggg ttgcgcatcc
     8701 gcattgcaga tctgattgac ggtccgctgc gttctgtcac gcagtggctc acgcgtgtgg
     8761 tatcaaaggt gacggcgctg gcgcaggccc atcccgcact gacgcgccag ctactgattg
     8821 caggcggtgc attgctggca atgactgcaa cggttggctc gttgtcgctg gctattggtg
     8881 tgcttgctgg cccgctggca aaactgcgtc ttggtttttc cctcctgacc ggatcaatga
     8941 atgctgtcag ggttctgcca gcactatggg gaatggtgac gggttccgtt tctttactgg
     9001 gaggcgctat cggggcgttg ttcagtccgg ttggtcttat cgtggctgcg cttgccggag
     9061 ctgccgttct tatctggaaa tactgggatc ccatcagggc attttttgcc ggggtgttca
     9121 gcgggattat ggaaaggctg accccgttgc gcgaaacctt tgaacggttt ggtcctgttt
     9181 ttgacgcaat cgggagtggg atcagccagg tgtttaactg gtttaaatcg ctgctgtcac
     9241 cgatggagtc cagcaaggaa actctggata aatgtaccag tgctggcgag atattcggta
     9301 acgttcttgg cggtgcgtta caacttgttc tgacacctgc aaaaatgttg ctggatacgc
     9361 tggcgtggat acttgaaaag cttggtgtgc ttccggatga agcggaaagg gcgcgcaaga
     9421 aaatcgaaga cgcacagcgt gcggccattc ttcaggacaa ggttgccttg cttcaggggg
     9481 atctggcgaa aatcaatccg ccgaagcctg tggaaaatgg caatggcacc ggaggtgata
     9541 accccaaaga caataaaccg ctcacagaca gcaataccgg tacgctgcgc agactcagca
     9601 aaattgctga taacacaggt aagctggttg aggagacgaa aaaacgcatt ggccccggcg
     9661 atattgtctt taaaaacctg ccccgagcac ttgctgttcg tggggagtgg caggagcgga
     9721 agattgcgca ggtcagtaag cctgcccccg caattaatat cacacccgtg gttccggctc
     9781 cgctgcctcc ggcgctggtc cctgttgttg cggccagctc ccgcccggtg gcggaggcta
     9841 tacgatctcc agtggcatca gttcctgtaa cttcccgtaa ccgggagcct gttgccttcg
     9901 gatttggtgg tgaaattcat gttcatctgc ataacgttgt tacgcagaat ccccgcgaac
     9961 tggcgaaact ggtcggtgaa atggtcaggg cagaaatgga acggcgcgcc cgtgccgggc
    10021 gtggctgttt ttacgataaa gattgaggag tcatggccat gatgatgatc tacggcatgt
    10081 ttgtttttga gctgcgcacg ctgccgcatc agcagttaca gcaaaacaaa agctggcggc
    10141 atgtgaaaaa tgaacgcgtt aaccgttcag caagctggca gtatatcggt gcaggtgatg
    10201 atcgcatcgt tctttctggt gtgctttatc ctgaaattac aggtggcgaa gtgtcgctgt
    10261 cgctgctgac tacgcaggcg tatacaggac gaccctggcc tttgattgat ggcgtcgggc
    10321 agatttacgg catgtatgtc ctgactgaaa cgaatacgac ccgctccgag tttgatcgct
    10381 acggtaaggc gaaaaagata gaattttcac tgactcttga acgctgtgat gaggatttgc
    10441 gggagcgcct gcaatcctca tcgttcagtg atatgctgtc cggcttcaaa gataaggtga
    10501 catcatctct taacagcgcg gccagttcag ttaaagggct gttctgattt aacacaaaaa
    10561 ccgctaatgg tcagattagc ggttttcatt ttcctgagtc tgcccggttg tttcttcagt
    10621 ctgtatatcg cctacagggt gataacgata aatcgtcgat gtgccaatgc cgtaaattat
    10681 tgccagttgt tttctgtcgt gcccgttttt gattagcctt gctatttgct catgctgttc
    10741 ttttgtcagc ttcggtcgac gtccgcctgt gcgccccctt gcgcgcgctg ccgccagccc
    10801 ggccagtgta cgttcaacaa ttaattcacg ttccatttca gccagggcac ccatcacgtg
    10861 gaagaaaaaa cgccccattg gagaagatgt gtctatgctg tcggtcagac tgcgaaaatt
    10921 aatccctcgc tcccgtagtt ccccgacgag agaaatcaga tgtttcatgc ttcgcccgag
    10981 gcgatccagt ttccagacaa ccagcgtgtc accttttgaa ggcgctttaa agcgcgtttt
    11041 aatcccggtc ggtctgtctt tgtcccgctt aatttatctt caaatatttg ttcacatcct
    11101 gcacaaacaa gagcgtttcg ttgcaggtct gtattctggt catttgttga tacccttaca
    11161 tagccaatca gcacactgaa tctcccgtcc aaaagcgcaa atcatgccat gcaggccaga
    11221 aacggccatt atttaaaacc tcggtttacg agaactcggc acaagcgggg agaaaatacc
    11281 gttactcagt acagcgaata cctggaccaa tcgacaaaca ttcagcggtg gcctttctgg
    11341 ggaactgtcc ggcaatgccg ctactgcaac aaagctgaaa acagcacgaa aaattgctgg
    11401 agttggtttt gatggttcta gcgatatttc aattagtgcc aaaaatgtca atgcatttgc
    11461 actccgacaa acaggtaata ctgttaatgg tgatacatcc gttggatgga attgggatag
    11521 tggtgcatat aacgccctga ttggtggtgc atctgcatta attcttcact ttaatataaa
    11581 tgctggtagc tgtcctgccg tacaattccg tgtgaattat aaaaatggtg gcatttccta
    11641 caggtcggct cgtgatggtt atggggttga attaggttgg tcagatttct ataccacgac
    11701 acgaaaacct tcagcgggag atgttggtgc atatacgcgg gcagaatgta actcaaggtt
    11761 tattacaggt attcgccttg gcggtctgtc atctgttcag acatggaatg gtcccggctg
    11821 gtctgacagg tcaggttatg tcgttacggg ttcagttaac ggaaaccgtg atgaattaat
    11881 tgatacaaca caggcaaggc caattcagta ttgcattaat gggacgtggt ataacgcggg
    11941 gagtatttaa cgatgatgca cttaaaaaac attactgctg gcaaccctaa aacaaaagag
    12001 caataccagc taacaaagca atttaacatc aaatggcttt attcagatga tgggaaaaac
    12061 tggtatgagg aacaaaagaa tttccagcca gacactttga aaatggtcta ttaccataac
    12121 ggcgttatta tttgtattga aaaggatgtt tcagcaatta atccggaagg cgcaagcgtc
    12181 gttgaattac ctgatattac agcaaatcgc cgggctgata tttcggggaa atggttgttc
    12241 aaagatggcg tagtgataaa gcgaacttat accgaggaag agcagaggca acaagcggaa
    12301 aatgaaaagc aaagcctgtt gcaacttgtc agggataaaa cccagctatg ggactcacag
    12361 ctacggctgg gcatcatttc cgacgagaat aaacaaaaat taaccgagtg gatgctctat
    12421 gcgcagaaag tcgaatctac agacacctcc agcctgccag taacgtttcc cgaacaacca
    12481 gaatgagaga aggcccgata ttgggcctta atttttactc aggcttttgt ggccattcgg
    12541 gatttgctgt atccacacgg ctgaccagaa cgctgtagcg ttcccatgac tccagtcgtg
    12601 tgcgttcctc atccatcgcc atattcagcc tgacagcgcg ttccagtggc tgaataacgc
    12661 tttcagcttc agaaagcaac gcgacctttt gtgattcggc ctgttgttgc tgttcgtctg
    12721 ctgtataaat ccgcttaacc acagttccgt ccttaaacat ccacttccct gaatcatcgg
    12781 cacgacgatt ggctgttata tctggaatct caacgacact aaagccttcg ggattaagcg
    12841 ttggggcatc tttggttatg gcaacaataa tattattttc gtcgtataca atctttatgg
    12901 tgtcgtcctg aaagttattt acttcctcat accagttttt accgtcttcg gaccataacc
    12961 agataacatc aaaattcttt gttagctgat attgttcttt tgtttttgga tttccggact
    13021 taatattttt taatatgctg cataatttac acctgtgcga cgttatacca tgtgccattg
    13081 atgtattttt gtattggtct gaagatggct tcatcatcgc catctacttc accaatgatt
    13141 cttaatccgg taattgcgtg tccggctttt tcataacgac caccacgcgc catcaattga
    13201 acaacacgcg tacccaggcg aacatctttt acatagcgtg aatcaaaatt cccccagttg
    13261 ctgggctgta tctgaccgtt aacagaaaat attaccgagt tatctgtatt tcgctggcta
    13321 tagaaatgcc atcctgcctc attgcctaac tctgcaacta ctggacgggt tgaagcaccc
    13381 cataaattaa acgttgcatt ctttgtggag ttgttggagc tggatagcgt gaatttttta
    13441 ccatccccgg cctgaatatt tttaaaagca atagccactc cattctgaaa gcggaataca
    13501 cgctgactat tagcataaac atccagaata ccgtctccat tctgtttaaa tccggtgtca
    13561 ttatcaccaa gaacaataga gctaccacct aacgcattcg tcgaaccaac tccaagactg
    13621 ccattaatga cagcattaac aagaatattt agtgcatccc atttcagcgt catcaggtct
    13681 tttgttgtgg tgctctggcg gcttctccat ttgaaatatt cattgccgtt gtcgcctgtt
    13741 tcaaaccaca tgtatgaatc agtgtcacca tcggcatcat ttttaaatcc aatcttcgcc
    13801 cagtcagtat ttcgaatcca ggcaaggatt gagtcatttt caaaagtaag cccaccggac
    13861 aaggtatcgc catttttttg cacggcgtta ccagccctgt ttaccgtttc ctgtaaaccg
    13921 aggtattcga taacggcagc aacggtcgat ttcgcaagaa tatcccgccc gacttttgtc
    13981 agggttgcca ggctggcgac atcattcccc gtaaaatacg gaaacctgtc tgccgcagta
    14041 gcaagcccgg ccagcgccgt cagggtggca tctttcggtt gcttacccgc aagcgcgtta
    14101 gtcatggtgg tcgcaaaatt cggggcgttt cccagcgccg ccgctaactc gttcagcgta
    14161 ttcagtgcgt caggtgacga atctacaagg gcggcaatcg cagccataac gaaagccgtg
    14221 cttgcgattt gggtattatt cgttccctgt cgcgcagctg gcgttgttgg cgttccggtc
    14281 agcgcaggac tgtttaaggg cgctttcttg ttcgtttcat ccattaccgc cttaaccgct
    14341 ttcgatgtcg cggccagtgt ttcagacgtg ctgttggtgg cactactgag ctggactatc
    14401 cctttttgtg ccgtcgtggc gtcctgagcg gtatattttg cgttagcaag gtcatacgcg
    14461 gcctttactg ctttcggcgt tgcagcctgc gtttcagacg tgctgttggt ggcgctactg
    14521 agctggacaa ggccttttcg cgtcgtgctg gcatcctgcg cagtatattt accgttagca
    14581 agatcatacg cggcctttac tgctttcggc gttgcagcct gcgtttcaga cacgctgtta
    14641 gtggcgctac tgagttgaac aaagcctttt gcggtcagcg aggcgtccgg gtgacgtcgt
    14701 gactgttcat gctctttcag tttgtcatcc acgtaatcca ctgtggccat caccatggtg
    14761 ttgtccacgg taagcgccac ggtggcagtg ctggatacgg tcagaatggt gcgaaatgtt
    14821 tgtgcacgac cggacccttc ggcaacggct ggcttgtaac tttcggcagt atttcccacc
    14881 gcgattaaat cgccgtgctc atcaaataca ccaatttccc ggatccagaa tccgcccgtt
    14941 tcaggaggaa taaccagctc cgcaataatg cggttctgat gtgttgcgtc caggatgacg
    15001 cgattaacag tatgtcgcca cacctcatgc accagacggg tctgcttact gtctggtgtg
    15061 ggcaacgtgc cgccaccgtc gcccacggcc atatgagtca ggcggacagg cttaccatct
    15121 ggcgcggcta cctgagctaa tttttttgca cccgtatcgg tgataatggt tttaaatttt
    15181 cgtgttgtgg tactcatgct taatcgcctg gataaatggt aataacttca ccgtcataag
    15241 ttgccgccgc tgcgaaaata tcccccggaa tttcctgaat gatattcagc cctgtcatgt
    15301 ggcggctgac cgggcgggca tcagcaatca accgctccat ttccagatac atttcctccg
    15361 tcacgccact gtccagcgtg ccgacttcaa cggtaaatgt tcccggttct ccgccgaact
    15421 cccaccactc agacacgcga atgaggtatc ccagcggctc aatggcccgg cgcagtgcgc
    15481 tgatggtccc tttgtgtcgg tgtatcagcc atgcatcacg aataacctgt cgctttgtct
    15541 cttccggcca gttgcgatcc cagcggtcaa cggaaaacgc ccaggcgaga taaggcagca
    15601 gatgcaccgg gcaggtgtcc ggcgaccaca gcgtgttgag gtctaccgga atgtctgtaa
    15661 tgcgtgttcc gacggcttcg gcacaacgca tgaaattgct ggctgatggt ggtaacaacg
    15721 aattactcat tgcgtccacc ttcgctgatg gtgaatgacg cacagcgcgc cgcctgtatg
    15781 tcgctgatgg ccatattctg tgtgggttcg attatctcca cgcgttgcac accgtgcaca
    15841 tgcagtgcgg cagcaatggc ggacaacgcc acgtcctgac cgataagccc ctgctcagcc
    15901 agccacttcc tgaacgacga ttcagccgcg gccagaatag gttcggattc cgggccgggg
    15961 taaaagtaca gttttgcatt cagccgccat gtcacgattc tggcactctg tacggtcagg
    16021 cggtcggcca ccgggcgggt atcctctgca ttcagaacgg cgcgaacggt attaagcaac
    16081 gcctccgttg ctgtgccgtc gccctcagtg gacaggatgg aaaccgtcac gttggccgga
    16141 gacgggctga tggcccgcgc atcgcgtacc agaccgctgg cgctgcgtgc aaaatactcg
    16201 tatgcacctg acgggccagc aacactcaga ccgtcatatg cccgttgcgc ccgtagtctc
    16261 agcgaggtgt cactttccat cactgcatcg gtggtatccg ttgccggagt gataaccagg
    16321 cgctttgtgt tcatattgcc cgcgaggttg tccaggtctg tcccggcgct gtggcttagc
    16381 atgcaggcgc gtgcaccctc attgacccgc tggcgtaaca gcatttcacg aaacgacatg
    16441 gtttgagcga taacgttaag gggttccgat tccagctcca gcgcggcgga aacggcttca
    16501 cgctgttcgg cgggatagga cgcaatcatc atggcctttg tgtcagccag aattgcctca
    16561 aagtcaggct ccgcgatgat ggcgggttcc ggtaactgtg aaaggtcaac ggcaggcatg
    16621 atttactccc tcagcgtgat ggttaattca acattctgca tggtctgcat gacagtgccc
    16681 gacagcgtca ccccggcgcg gcctcctgcc ttccagacaa cgtcgatagc gtccagggca
    16741 atgcggggtt cccatcgtgt cagcgcaatc acggcagcac tcatgcattg cagacgcgtg
    16801 gtgttattca tgggttcgtc aatcaaatca ggcacaaggc tgccatattc ccgtcgcata
    16861 acccggcttg ccagcggggt ggtcaggatg tccctgactg actgtttcag gtgctccata
    16921 tcgttcaggt ttcccgttcc gtccgggttc attcctgtgt agcgggttgt cactgcgggc
    16981 ctcctgtcga atcgctgcca cctttaacgc caccgtgttt atgcgtatgc actgtgatgc
    17041 cgtttgaggt gaaattaccg ccgctgtgcg tgatattgcc gctcatcttt cccccttttg
    17101 tgacgtcaag cgtcgccgtt ctcagaaggt ctgtgcattc cacgacgggc gtatccagtg
    17161 tcacgctgac ggatgcctgc agggtggctg ttttcatgcc gctggcgctc agtgcgcctg
    17221 cgtccgcgtc gtagcggaac accgcgccat ccggcgcgct gaccacgatt tctttcaggc
    17281 ttttgccggg ggccggaatg gcatcactcc acaggctgcc aattatcatg gcggtttccg
    17341 ggttgccgcc aatgcaggca attaccacct gttcgcctgg tgatggcggc agccacacat
    17401 tgaaggctcc cgcgcgagag gtgttccagc gcagccaacc tgtttccagt tcgccgctgc
    17461 gaacgcgcac gcgccaggac ttctcatcaa cttcagagat gatcccggtg cggatgatat
    17521 tgctcagtag ccgcatgagt tctgcgctca ccgtacagcc tccgcaatcc ggcccagcac
    17581 cgtgttataa atcaggcgct catctgcctg gctgataccc agcagctcac gtaccgggta
    17641 atcggtgaaa atgcccggcg caacctgatc gcgctcaccg aactgatgaa cgcgtgcaat
    17701 acgtgcggcc acgccgctgt aacccaccgt cacaccggaa gcatctgcac gggctttcag
    17761 gtagcgggcg gtgcgcagtt ttacgaacat ggggacgcgc tttgtgctgt cctggttgat
    17821 gcgccgggtg cgtatttcca gaaagcggtc gatgtcatcc cggtaaaacg tgcggatgct
    17881 gtttttatcc tcatcccacc cggtgatggt tcgcccgtat ttccccgtgt cgtgatgcca
    17941 gtttttcagc gtgcgtgctt cgttattcca gataaagcga atgcgctcct gtatccgggt
    18001 tacgtggcgt ctgcgtggtg tccatgcggt cccgtccggc gctttctgtg gccggatacg
    18061 tgcctgctgg gcgcggcgta aatcctgtgc cagctttctg gcgatgttat tgatggcctg
    18121 ctgattcagg ctgtcgcgga tggcctcaaa ggtttcatcc acgcgagtga atgccttatc
    18181 catcgctttc accccacgtc acatcctgga atacatgcga ccagtcgcct tcggaagatg
    18241 gcaggcgggg ttttggctcc agcaggtgtt ctgcctgcgg tgtgccctga ctgctgcgcg
    18301 tgatgcgaac gcgttcccgc agggggagcg taaacaggag atcggcgctg tcatcgtcat
    18361 tgataacggc ggaaaatttg atgtcctgat tacgctcagg gttgagcaac aactgtggct
    18421 gattttcgga taaccacgcc agcagcggca gcgtgaggtc gtccagctcc ccggcgtaat
    18481 ccatgacaaa catcaccatc tgatagcggt aaacaaacga tggggtttct ccggtcgttt
    18541 caatgttgcc gctctccacg aaaatggtga atttttccgg gttagcctga caccatcggc
    18601 atgaacgggt catggcttca cgcagggaat cagttttcag catggttgtt atcctcgttg
    18661 ttcagtcgtt gcagcctgcg ctgttccagt aattcaatgg cccgtttatc cgcgttacag
    18721 gtttccagtg catccagaag gcggtcgccc catataccga gatttcccca tgtgggagtg
    18781 tcagggaagg ggggaggcgt taccggtatg gtcagcgtct gcggtataag ccggactgac
    18841 ggcgctggca gtggcgcgtt ctgcgtgcct gcgcagcctg tcagtaaaac gagcgtcagg
    18901 caaagcgtgg gcgcattcat cttttgcaat atcgttgcgt agctgttcac gtcttacctc
    18961 tccgtcctga ttgcgttgct gattttccac gcggagttgc gccagcacct gctgcatatc
    19021 ctgtaccccg gcgctgatga tattcagggt gtcggcggta cttttcaggg tgctggcctg
    19081 cgcttcgttt ctggcgttct cccggcccag cgaccacgac agacgcatgg atgttcccca
    19141 tccggcagtc agaaggaaag cgacgccaag cgtgggccag agcttcatgc cggataggct
    19201 ccgtgtggta actgaaaatg cggtccgtct ttcagggtct tccagtcgcc gccccattcc
    19261 accggaatat tcagttcccg gctggcctgt ctgaatgctg ctgcgatttt ttcgtacagc
    19321 ggccattccc atgacatctg gctgccgata taagccacaa catccacggc atgccccgta
    19381 aggtggcggc tgttcatggt ctggctctta cccgtggcca ccagttgctt ctggcggtaa
    19441 cggctgcgca acccttcggt gataccaaaa tccacttccg agatttccag tgcccgtcgg
    19501 gtcactttca ccagatcagg atttacaccc tgcaaattct tttcgctccg gctgctgaat
    19561 ttaaatgtgt tgctcattcg tccttctcct tcaccctgcg attaaaggcc gcaataacct
    19621 tgtcgcgtgc tttctctgca cccataaaac cgattgatgc gccgataaac gtcacggcat
    19681 cttcaggaaa cccgaagaag cgcaacgacc cggccacggc catggcaaga acgccgcacg
    19741 ccagcgatcc cgttacggtc tgaaccagtg ttcgtccgtc ataaaggctc atcagcgcgg
    19801 aaatgctgac cgccgcgcct actgcataca ccgttggcag gtggtcaaag agccacgcaa
    19861 taacctgctc tgtgatccct gtttgaatgg tgctcactgc tactcccccc acaactgaat
    19921 catttctcgt ttcttcttct ccggctccgg catctccact tcctgcccgg cgtccagaaa
    19981 tacctgctga cagagtccgg ggttggcatc cagcaccttt tcggtgacgc cctgcgtcgt
    20041 gccgtagtac cggaaacaga gcgaatccac ggtgtcgcct tccagtgcct tcactttcat
    20101 cagcacaact ccgcaaagat tcgcgggcgg cacagaatgt cagagatggc ccagctcaca
    20161 tcacgccaca aatccgatgt ctgtatatcc agagcgtctg cccggcggtc gcccttgtcc
    20221 gtagtgtctg catcacgata acgctccaga atcagggcgc gcgtggcggt ataaacagca
    20281 ttgcgccagt gccagagatt gacgctttct ccgttaatta cgggtgccgg aacatcggcc
    20341 agcgtctgat ggccagctgc ctgctgttcc tgctgccatg cttccagctc gcgggtaacg
    20401 tgtgccacag ccccggtggc agtatgcagc aggcgggagg tggtcacgcg ccccggcagt
    20461 cgtaccgcca gacgcagctc gcgcagcaca atatccggcc agaatgcacc cgctgaaatg
    20521 cgggtatcgc catcatcggt atcggtgatg tcgtcctctg cgggtccggg ttcagttctg
    20581 gcaactatag tcatggggtt cactcctgaa aaaaaatcgg gcggtgggtg cgcggtgtaa
    20641 acggtcacgg agtcaaactg gaacaccgcg cacgccgccc gctgacgggg tcagtcgtta
    20701 accgcgcttc gccttctgcg tcgcggtggt ttttcgtgtt gcaggcttcc gcgttgtctt
    20761 tttacttttg ctgctttttg ctgctttcgt cctgcgcctg ctgtgcgctg gcgtcttctg
    20821 gttcggctgc ggaatcggct tttttcaggg cgcgggaaag ggttgcaatc tcgcgtttca
    20881 cacctgcgtt cgggttcagg tgcattgctt cgcgcagcag cttcagtgac agggccatgc
    20941 tgtccgcatc actcaggcca cggcgggcaa aggcgcacgc tttgcataat ttggcgcgca
    21001 cttcgtccgg catgtcctgg tcggtgacaa tctcccggag ggtgtccagt ggttcgataa
    21061 aggcggacaa atccgcgtcg gcatccgtcc cggcctgcgt cagtaccgga ttacagattt
    21121 cttcggtcag taccgtggca gcagtacggc caaagttatc cggcatgatg aggttgtgac
    21181 ggactacata cgcgccgata cgcagcgcaa gcggaagatc gccacagtca atcgcccaca
    21241 ccatcagcgt ggcaatcact tcatcctgct gcccgccgtc agcctccagc gttccctcaa
    21301 tccagccgga aaaatccggc aacaactctt ttttgatggc ggctttcgcg cttctggcct
    21361 gtacgccctt aagccgggcc tgtgccagac gcagacgata cagcacctct tcatgcgcgg
    21421 tacgcgcggc gtggtccacg ccttcattcg cccggcctgc gcgctgtgcc atcacgttct
    21481 gccagtgttg ttgtgcagga gtaatcattt ttgctctccg ttacaggcgg gcatgatgcc
    21541 cgccgtgagt tgattagctg tcggcgaact tcaggccagt aaccatcgcg cacttgccat
    21601 agtcttcaac gacataagcg tcattgatgg actggtaggt ggcgatgcgg ttgtattccg
    21661 gctcgtcttt catcaggcga cgcattgaac ctttctgcca gtaaatcgac aggttgttga
    21721 acgaggtgat cagcatcgtt gcatccggga agaacggcgc aaggaacacg cccagcccgc
    21781 caatggtgcg cgatgacagg atgagctgcc cggcgagtaa ttccgcattg ggattctggc
    21841 cgctgatgct gttcagcacg ggcagacgca gcgagttaaa caggttgcgc cccataatca
    21901 ccacgaggtc gtcagcttcc ttgtgccatt catctagcag ggatgaacgc gcgtcctgta
    21961 ccagtgcatc agcgttcgca tacttacccg cgtgcgccac ggtgttgtcc atgttgcggg
    22021 aggtcagcgt cacgtcattc ataacgcgtt cgctggcgtc ggttctgatg tgctccagcc
    22081 agcccacgtt aacgtcctga agcagcttgt tggtgctgaa gttggactca tccgcgtgag
    22141 acgtgccgtt gaaaccgatc atgatgcggt caagcgccac ctgtcgggca atctgtgcgc
    22201 tgatgcggga ctgaaaatca gggtgtgccg accaggcatc aagctgcgga tacgaaataa
    22261 acgtgtcgta gttcacctgt tcacactgat atttgcggtt tttcagatca accacgttat
    22321 tcgggttacg gcgttttgtg ccgtcataac tggtattcgt gcgcgcaatc ggtcctgtgg
    22381 tatccaggag gattttttcg cctttctggt cggtcacgcc gatcacgtta tttttttcgt
    22441 aaattcggta ctttcctttg aggcgttttc aaaacgctgc tgcaccgcgg gttccacggt
    22501 aaatcgcgac accagtgcag ataccgggat attattaagc gacacctgct gcgccatata
    22561 gcaacccagc ttgttgcggg taatatctga catcaccaga ttcataaaaa atttgctcct
    22621 ttgtctaatc agaagtcggc cagctggtcg gaggctgcgc ccgttgcggt gaagcggttc
    22681 tgcggatcgc cgtcctgcgt gcgcagtttt tccttcagtg ctgccagctc tgtggtcagt
    22741 gacgtaattt tctggcggtc ctgctgatgg cgggtttcca gcacattaaa acggtcgata
    22801 atgtcggcct gtgacgttgc gacgccttcc accgcttcct gaatacggga aaaactggcg
    22861 tcatccgctt tgcggccacg gccaataatc cccattacgc ggttaaacca ctgggtgcct
    22921 tcttcctggc gttgttctgc catttcgatg atttcagact cgatggcttc ggagataagc
    22981 ggcgcttcac cctggatact gttaaacgtc atcaccgcct aacgttgctg tgccgtgaat
    23041 ttcaggcgct cagtgcccag gcttgccggg gtgtcggtca tcgccagccc gaccagatag
    23101 gcgcgcccgt taacggagaa ctgcgggtgc agttcgatac tggaatagat tttcttgccg
    23161 tccgcaacaa gctgcttcat gcgctcggtc ggttcgattt ctgcatacag cgcagtacgt
    23221 ccggccagcg gaccttccgt aatgtcttcc gtactcagtg cggtgacatc gcccattgcg
    23281 gaaaattcgc ttgacgggca tggcgagaga tagtgctcaa cgttcacgcg ggcagcgtaa
    23341 acatccgggt tgaagttctc ggcggcttca cgcagatgca ccggactgat ttcacggcca
    23401 tcaacagttg atccggagac agccacgcga aactttttgc gggatgtctt tttttcatta
    23461 gccatagttt ttgcccctct gactggttct tcagtcatga tggcaaagcg taacaggctg
    23521 atacaaaggg cttttgttgt aagaaaacgg ccagaacaga gggttaagga gaacagtttc
    23581 gcgcgcgggt aatcttcctg taattactca gggggagcaa tgattcagga cgcttttgtg
    23641 cgccagcgtg cgcggcaact ttactggcag ggttatccgc ccgcagaaat atcacgtctg
    23701 atgggaataa acccgaacac gatttatgcg tggaaaaaac gtgaccagtg ggatgaaacg
    23761 ccacccgggc agcgtgtcac gcagtccatc gatgcgcgcc tcatccagct tactgaaaaa
    23821 cagaataaaa caggcggtga ctttaaggaa atagacctgc tgacccggca gcttaaaaag
    23881 ctgcatgatg gccagccgga tgtgatggcc gcaggaaaga aaggccgggc gaaaaaactc
    23941 aaaaatcatt tcacgccgga acagattgcc gcactgcggg aaaaaatcat cagcaggctg
    24001 gagtggcatc agcggggctg gtttgactcc ctgacccttt gcagggaagc cgggatacgt
    24061 aacaggatga tcctgaaatc ccgacagatt ggggcgacct ggtattttgc acaggaagca
    24121 ctgctgatgg cgctgcgtga cgatgtggcg caaccttacc agcgtaacca gatttttttg
    24181 tctgcgtcgc gtcgtcaggc gttccagttt aaaagcatta ttcagaaggc cgcggctgaa
    24241 gttgatgtgg agctgaaagg gggcgataaa atcatcctct ccaacggcgc agagctgcat
    24301 tttctcggca cttctgctgc gtcggcacag tcctatacgg gcaattttta ttttgatgaa
    24361 tttttctggg tcagtcgctt tgctgaactg cgcaaggtgg ctggcgctat ggcaaccctc
    24421 agcggactgc ggcgcaccta cttctccacg ccatccaccg aaacgcacga ggcatacgcc
    24481 tactggaacg gcgaccgctg gaacgagaaa aaggcctcgc ataaacgcca gcgtttttct
    24541 gtggactgga aaacgctgca taacgggctt atctgccctg accggacgtg gcggcaaatt
    24601 gtcacgctgg aagatgtggt taatcacggc tggaaacaca ccgatatcga cgaaattcgt
    24661 gatgaaaaca ccgaagacga gttcctcaat ctctatatgt gtgagtttgt ccgcgaaggg
    24721 gaatcggcat ttaacctgaa tatcctgatt ggctgcggcg ttgacggata cgacgactgg
    24781 aaagactgga aaccttttgc tccccgcccg atggggaatc gtccggtatg gattgggtat
    24841 gacgcaaacg gcagcagtgg caacggcgac agcggcgctg tgtccgtggt ggttcctccg
    24901 tctgttcctg gtggccgttt tcgaacggtg gagacgcgac gcgttcaggg gctggagttt
    24961 gaagaacagg ccagagtcat tgaagagttc acgtgtcgct acaacgtgga acacatcggc
    25021 attgatgtga cgggcgggaa cggggaggct gtttatcaga tagtgaaacg gtttttccct
    25081 gccgctattc cgtacacctt cacgctgtca tcaaaacggt cgctggtact gaaaatgctg
    25141 caaataatgc gtgccgggcg gtgggaatac gatcgcgccg aacgcgagct ggtcgcggcc
    25201 tttaacgccg tgcgtaaggt gaaaacaccg ggcggcttta tcacttacga aacggaccga
    25261 gcgaggggga tcagccacgg cgaccttgcg tgggcaacca tgcttgctgt cattaacgaa
    25321 ccgattggcg gcgaaggaga aaacgagcgt ttcacggtta tggagttctg atgagcagaa
    25381 aaaataaaaa agtgcgcatg agttcacgca ttgatctcgc tgatgcgctc aggaaagaat
    25441 catcgctcag tgcattcaca tttgatggtc cttatcgcct gacctgctgg acaatatgta
    25501 ctgtgctgat aacgggcggt ggtatgaaac cccggtggac tggtacggtc tggcaagagc
    25561 tgcccggcaa acgtcctggc atcagtctgc gctttatttt aagcgcaatg tattgctcgg
    25621 ttgctacatc ccgcacccgc tgctttcccg gcaggatttc tcggcgctgg cgctggactg
    25681 gtttgtgttc ggtaacgcat tccttgagct tcgaagcaat atgctcggcg aaccgcttaa
    25741 attacggcac gccctggcga aatacatgcg acgcggaagc gatcttgaat catggaggta
    25801 tgtgcaggat ggcaaggatg cgtttcagtt tcgcccaggc aaagtgtgtc acctgatgaa
    25861 tcctgacatt aaccaggaaa tctacggcat gccggaatat ctcggcgcat tactctcggc
    25921 cagcctgtct cattcggcgg acatgttcag aaaactgtat tacgacaacg gatcccacgc
    25981 cgggtgcatc atctacatcg gtgccgcgca ggtaaaccgc gaaagcatgg actccctgaa
    26041 agaaacgcta cagggtgcgc gtggtggtgg tgcgtttaaa aacgtgctca ttcatgcgcc
    26101 caacgggggc aaagaggggg tgcaaatttt gccgttccag cagatcaccg caaaggatga
    26161 gttcatgaat gttaaggcgg catcccgtga tgatgtgctg gctgcgcacc gcgttccgcc
    26221 gcaactgatg ggggcgatgc cgggtgaaaa aagtgcgttt ggtgatgtgg agaaggccgc
    26281 gcgggtttac gcaattaacg agctgatgcc cgtcatggag gccatgaagc acatcaatga
    26341 ctggcttggc gaagaggtga tccgctttaa cccttacgca ctgttagaca tccagcccac
    26401 atcctgacgc gcttcgcttg tctgctgctt cgccggggca taaaaaattt atgccccgac
    26461 tctccagctc ctgtatcagt cagataattt cacgacgcct tccagtttat cgccaccatc
    26521 gacggtcaga ctcttacgca atcccaccgc gttgactgca tgttctcgcc gcctcagtgc
    26581 gattttgacg gccttacctt tcaccccatc aaatcagaat ccctcacgta tttttcacgc
    26641 tcagcgtgag aaatacggcc attctgtcgt gtcgctgcga catcgttaag ggaacgctat
    26701 ttaccccctg aaacgcgggc tgttcccccg tcacctgcgc gcagaaaaag cgcgtttttt
    26761 tgtgcacgca cggatccttg acggatccag ccgccacgcg ggctggaagg gcaaaaattc
    26821 gttcaaaaaa attgtgcaaa tgtgcagcag ggcgatgtgc gattatcgcc ctggtttgag
    26881 ggggatcagg tgttattttt atctgtcgtc agtgggctaa accatccaca atttacatcg
    26941 catgactgat gcgttctgtc ctcgtgtttc tcctgccaca atgcttttcc gccagccttt
    27001 atgatggctc tttcaacatc ccgctcgtat cgcaagggct gaaatacgtc attaatgaaa
    27061 tattcgctta tgtccggaag cgatactgca aaattatttg tttgttgcgc cgtcctgcta
    27121 tgcagcaaag cggcatgctt taatgcgtcc tgtgctttct ggaatttaag tgcatagtca
    27181 gtggcgattt tttccagttc agcgattcgg ttgtctttgg tttccagcca atcaagtagc
    27241 gccaatactt caggatcccc gacatccaac actgttacgc gtgatttttc ataatgtttg
    27301 tcagcaagag ttcggccaat tttgaaatct ccatcatcac cataaccgga gcaggcataa
    27361 acaacctgtg ctccagatat tcgctgaatt gacatttcct cgccgcaaat agggcatttc
    27421 ggtactggca caggtgaata acgttcacgt aatgcctgat agttgatttc gctcattaag
    27481 tcgttctcct tgtttatgtt ctgaggtgga gagcagggcg aactcgccct gatgttgtgt
    27541 tattcaggaa ataacgcccg gatatttccg gccatctgac tggttatctg tgcggttgat
    27601 actggctgtg atgcggggcg ttctgtcctg gtttgtgtca ctgataacgc ttcatcacca
    27661 gcccatgcag ccagtcgata agcctctgcc ggattcattt tcagaagtgc cagcccggcc
    27721 agaaaagcca cgcgttggcc gcttttgcgg gcttctggtg taaggctgtc cagccaggcg
    27781 catgcttctc cttcgttctt gacggcggtg ggcttcagat agaaacttat ccgtctggtt
    27841 ggagtcgtca ttggtttact ccttgtccat tgcgtacagc ccattaacca gagcaaactg
    27901 tggcaccccg tccgcgatga aagtcgcatt aactccgcag gcttcgcgga tagcgggtgc
    27961 cacaatctcc gcccctccac cgacaaccat cacccgcccg taacccgaaa aaaccgccag
    28021 cgcgcggatc acgcgttgtt tcagtgtttc ttccttttca cgaattaccg ccatcaggct
    28081 ggcgtaatgc gcgtcattgt ggatgtgctg gcgcagccag gcttcatcat ggcgatgttc
    28141 gataatggta ttggcgatgt ggtgactggt gcgcataccg ttagtggcca tcaccgacag
    28201 tacggcatcg gccatcagag aaacgcccac gtgtggatcg caaaacacct ggctgatacc
    28261 tgccagttgc ccctgaacct ttgccacatc cagcgtggtt ccgcccaaat ccacaatcag
    28321 cagggattca aacggactca tgtcagccag tgccttaaag ccagccggaa tggattcagg
    28381 cataacctgt acgttacgga tagtgaatgc ttcgccgttc tggtactcca ccgggcgcat
    28441 gacgttcgct tttttgc
//
LOCUS       DAJVOY010000033        28244 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVOY010000033 DAJVOY010000000
VERSION     DAJVOY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.033"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(212..1312)
                     /gene="chaA"
                     /locus_tag="OQL90_001295"
     CDS             complement(212..1312)
                     /gene="chaA"
                     /locus_tag="OQL90_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1368132.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1582..1812
                     /gene="chaB"
                     /locus_tag="OQL90_001296"
     CDS             1582..1812
                     /gene="chaB"
                     /locus_tag="OQL90_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1368133.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1970..2665
                     /gene="chaC"
                     /locus_tag="OQL90_001297"
     CDS             1970..2665
                     /gene="chaC"
                     /locus_tag="OQL90_001297"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1368134.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2709..3062)
                     /gene="ychN"
                     /locus_tag="OQL90_001298"
     CDS             complement(2709..3062)
                     /gene="ychN"
                     /locus_tag="OQL90_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1368135.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3246..4641
                     /gene="ychO"
                     /locus_tag="OQL90_001299"
                     /pseudo
     CDS             3246..4641
                     /gene="ychO"
                     /locus_tag="OQL90_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
     gene            complement(4642..5292)
                     /gene="narL"
                     /locus_tag="OQL90_001300"
     CDS             complement(4642..5292)
                     /gene="narL"
                     /locus_tag="OQL90_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1368136.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5285..7081)
                     /gene="narX"
                     /locus_tag="OQL90_001301"
     CDS             complement(5285..7081)
                     /gene="narX"
                     /locus_tag="OQL90_001301"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1368137.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRVRLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7420..8811
                     /gene="narK"
                     /locus_tag="OQL90_001302"
     CDS             7420..8811
                     /gene="narK"
                     /locus_tag="OQL90_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1368138.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9327..13049
                     /locus_tag="OQL90_001303"
     CDS             9327..13049
                     /locus_tag="OQL90_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1368139.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13067..14605
                     /gene="narH"
                     /locus_tag="OQL90_001304"
     CDS             13067..14605
                     /gene="narH"
                     /locus_tag="OQL90_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1368140.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14602..15312
                     /gene="narJ"
                     /locus_tag="OQL90_001305"
     CDS             14602..15312
                     /gene="narJ"
                     /locus_tag="OQL90_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1368141.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15312..15989
                     /gene="narI"
                     /locus_tag="OQL90_001306"
     CDS             15312..15989
                     /gene="narI"
                     /locus_tag="OQL90_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1368142.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16172..16303)
                     /locus_tag="OQL90_001307"
     ncRNA           complement(16172..16303)
                     /ncRNA_class="other"
                     /locus_tag="OQL90_001307"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(16351..16435)
                     /locus_tag="OQL90_001308"
     tRNA            complement(16351..16435)
                     /locus_tag="OQL90_001308"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16399..16401),aa:Tyr,seq:gta)
     gene            complement(16470..16554)
                     /locus_tag="OQL90_001309"
     tRNA            complement(16470..16554)
                     /locus_tag="OQL90_001309"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16518..16520),aa:Tyr,seq:gta)
     gene            complement(16714..17556)
                     /gene="purU"
                     /locus_tag="OQL90_001310"
     CDS             complement(16714..17556)
                     /gene="purU"
                     /locus_tag="OQL90_001310"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1368143.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(17606..18064)
                     /locus_tag="OQL90_001311"
     CDS             complement(17606..18064)
                     /locus_tag="OQL90_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1368144.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            18177..19082
                     /gene="rssA"
                     /locus_tag="OQL90_001312"
     CDS             18177..19082
                     /gene="rssA"
                     /locus_tag="OQL90_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1368145.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRVLGADIVIAVDLQHDAHLIQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            19174..20187
                     /gene="rssB"
                     /locus_tag="OQL90_001313"
     CDS             19174..20187
                     /gene="rssB"
                     /locus_tag="OQL90_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1368146.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            20389..21297
                     /gene="galU"
                     /locus_tag="OQL90_001314"
     CDS             20389..21297
                     /gene="galU"
                     /locus_tag="OQL90_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1368147.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21441..21854)
                     /gene="hns"
                     /locus_tag="OQL90_001315"
     CDS             complement(21441..21854)
                     /gene="hns"
                     /locus_tag="OQL90_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1368148.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            22459..23076
                     /gene="tdk"
                     /locus_tag="OQL90_001316"
     CDS             22459..23076
                     /gene="tdk"
                     /locus_tag="OQL90_001316"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1368149.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(23358..24254)
                     /locus_tag="OQL90_001317"
                     /pseudo
     CDS             complement(23358..24254)
                     /locus_tag="OQL90_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24378..27053)
                     /gene="adhE"
                     /locus_tag="OQL90_001318"
                     /gene_synonym="adhC"
     CDS             complement(24378..27053)
                     /gene="adhE"
                     /locus_tag="OQL90_001318"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1368150.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            27531..28178
                     /gene="ychE"
                     /locus_tag="OQL90_001319"
     CDS             27531..28178
                     /gene="ychE"
                     /locus_tag="OQL90_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1368151.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         6935 a         6786 c         7365 g         7158 t
ORIGIN      
        1 ctacgtctca attcatcttc acttcatccc tgaaatgttt gcaataaaga gtacattccg
       61 gcttttcaac agctgttgca gtggtttcgt gcaggttgtg gtacaggctc gcaattctga
      121 ttaacgagct gtccaggtag tatgaagaaa tacaataagc aggaatttat cttatctccc
      181 ccgatttccg tcatcggagg agatataact gtcaggcaaa tatcgtcatc aaataggcgg
      241 caaacagtgc cagatgcgct gcgccattga gcacgttagt acgtccggtg gagaaggaga
      301 tatggcacag cactaaagag gccaccatca ccaccatttc tggcgcacca agtgcaaact
      361 gtaattcgtt acccgtcata aaggcaatta gcgtgacgac aggtacggta agtgaaatgg
      421 ttgctaacac tgaaccaaag aacagattca tcgcgcgctg gacctggttg ttcaacactg
      481 cttttaacgc acctaaacct tccggcgaca gaatcaacag agccaccagg aagccagtaa
      541 aggcgacagg ggcattcatg ctgtcgagca atgtctccag cgggctggca ttcattttgg
      601 tgactgcaat aacggcaatc agatggataa tcaaccagat agcatgccac acgctgctat
      661 gggcagacgg tttaccgtga tgcgggtcgt cgtcatcact gtcatcttcg tgctcgtaga
      721 caaacaaact ttgatgcgtt ttggtctgga tcagcaaaaa tacgccatac attgccgcag
      781 aaattaatgc taccagtaac gcctgaccgg ttgaaaaatt cgccgcaggc agagccattg
      841 gaaataccag tacgattatc gccaggggga acagcgcaat taaatactgc ttgataccaa
      901 acagattcat atattgggtg gcaaacttac gaccgcccaa caataatgaa aagccaacca
      961 gcccaccggt aacaatcata atgattgaat agagcgtatc acgcattagc gttggcgcgg
     1021 cgtcgccggt tgccattaaa gctgaaatca aactgacttc aagaataacc actgaaaggc
     1081 taagaataag cgaaccgtaa ggttctccca ggcgatgggc taatacgtcc gcatgacgga
     1141 caacactaaa ggcgctactt aaaataccaa taagcgcaag aagattgatg gcaatgacca
     1201 ctggtagtgt ctggctgctt ccccacagga acagcactac cagcgccaga accgggaaaa
     1261 taagcgaaat ctccttgtgg cgggttttta ccgcctcttg agcatttgac attatggtta
     1321 tccctttgca gatgaattta tcgaaaatgt aaaaaatagg taggaaaaat aacagaaagt
     1381 gtctggatat cggtaacatt ttacgaattt ttaccccact gtcgattttt tactcattgg
     1441 gacataaaaa taagtattac gtttagacaa tgtttgtttt agcgtttctt aagaacagtc
     1501 tgacatgaaa attcttatgt tttggcaaga gtagatattg ttgaccacac ttaatgttca
     1561 actttgtaaa aggagtcaac gatgccgtat aaaacgaaaa gcgatctgcc ggaaagcgta
     1621 aagcacgttc taccgtctca tgcccaggat atctataaag aagcgttcaa cagcgcatgg
     1681 gatcaatata aagataaaga cgatcggcgt gatgacgcca gtcgcgaaga aaccgcgcat
     1741 aaagtggcct gggctgctgt gaagcatgaa tatgccaaag gggatgatga taaatggcat
     1801 aaaaaatcgt aaaaccggta gcttagttaa agctattcgt gcggtgttgc cttgcaagtg
     1861 gtccgtggat tgcatattgt cccgttagtg gtttcaaaat gagcagtaaa aatgtccgga
     1921 agacaccaaa aagttgtcgc agggaagtat gcagtggcgg aggtgtaagg tgataacgcg
     1981 tgatttcttg atgaatgccg attgtaaaac ggcatttggt gccattgaag aatcactctt
     2041 atggtcagca gagcaacgag cggcttcgct ggcggcgacg ctggcttgtc gacctgatga
     2101 gggaccggtg tggatcttcg gttatggatc gttgatgtgg aatccggcac tggagtttac
     2161 cgagtcgtgc accggtacac tggttggatg gcatcgcgcg ttttgcctgc gcctgaccgc
     2221 cgggcgcggg actgcgcacc agccgggacg gatgcttgca ctgaaagagg gcggacgcac
     2281 tacaggcgtc gcctatcgac tgccagaaga gacgctggag caggaactaa ccctgttgtg
     2341 gaagcgcgag atgattaccg gctgttatct gccaacctgg tgtcagcttg atcttgatga
     2401 tggatgcaca gtaaacgcca ttgtgtttat tatggacccg cgacatccag aatatgaatc
     2461 tgacactcgc gctcaggtca tcgcgccgtt gattgcggcg gcgagcggtc cgctgggaac
     2521 caacgcacaa tacctgtttt cactggaaca ggagcttatc aaactgggaa tgcaggacga
     2581 tgggctgaat gatttgctgg tatcggtaaa aaaactgctg gcggagaatt atccggatgg
     2641 tgtgttacgg ccgggattcg cctgagtaaa cttcccgcat agtggggcgt cagacgcccc
     2701 ctcaaacatt aaaatgtgag cactttatcg gctgacagcg tccattgcgc cagttccaca
     2761 agagtaccga tttccacccc atcaatcaga ggaagtgtac taatcccgcg cccgtcggta
     2821 caggttttgc acaatttcac cggtacattc tgagcggtaa ggatctccag catttgctga
     2881 atgttgtagc cttcccctgg tttttgcccg cgcaacccgg ctgtgaccgc atcagacatg
     2941 aggaacagac gcagatccag attgctctcc tgctctcgta acgcaatggc cagccgcaag
     3001 ctgttaaaca aggattcgct cccgtaaggt gcgccattgg caacgatcac gattttttgc
     3061 attatttact cctgtattca gggaattaga cactcatctt ctatcttact gcttctgcag
     3121 cgtctgacca atcggtcaca tttttaagga ttttcctgaa agcgcgagaa aatacgacaa
     3181 aagttgccag taatcgttat tctttaaggc tatggttttc attattaccg gaagttaccg
     3241 acgttttgag ccgtttcgtt cctcgcatta tcccgtttta tttactcttg cttgtagcag
     3301 gcggtacagc taacgcacaa tctaccttcg agcaaaaagc ggcaaatccc tttgataata
     3361 acaatgatgg tctgccggat ttaggcatgg ctcccgaaaa tcatgatggg gaaaaacact
     3421 ttgctgaaat tgtgaaagat ttcggcgaaa ccagtatgaa tgataacggg ctggatactg
     3481 gcgagcaggc aaaagctttc gcattgggaa aagtccgcga cgcgcttagt caacaggtta
     3541 atcagcacgt agagtcctgg ctatcaccgt ggggaaatgc cagtgttgac gtcaaagtgg
     3601 ataacgaagg acatttcacc ggcagtcgtg gaagctggtt tgtgccgtta caagataatg
     3661 atcgttattt cacctggagc cagcttggtc ttactcagca ggataatggg ttggtgagca
     3721 atgtgggcgt tgggcaacgc tgggcgcgcg gcaactggct ggtgggttat aacacttttt
     3781 atgacaactt gctggacgaa aatcttcagc gagcgggctt tggtgccgaa gcgtggggcg
     3841 aatatttgcg attattggca aacttttatc agccgtttgc tgcatggcat gaacagacag
     3901 ccacgcagga acaacggatg gcgcgcgggt acgacctgac agctcggatg cgcatgccgt
     3961 tctatcaaca cctcaatacc agtgtcagcc tagaaacagt attttggtga tcgtgttgat
     4021 ttgtttaact ctggtacggg ttatcacaat cccgtcgcgt tgagtctggg attaaattac
     4081 acccctgtgc cattagtcac tgtgacggcc cagcataaac agggtgaaag tggcgagaat
     4141 caaaataacc tcgggctgaa tcttaattac cgctttggtg taccgctcaa aaaacaactt
     4201 tctgcgggcg aggttgccga aagtcagtcg ttacgtggta gtcgctatga caatccgcag
     4261 cgaaataatc taccgactct tgagtaccga cagcgaaaaa cgttaacggt gtttctggcg
     4321 acaccgccgt gggatctaaa acctggcgaa acagtgccgc tgaaattaca aatccgcagt
     4381 cgttacggta ttcggcaact gatttggcag ggcgatacgc agatattaag tttgacgccg
     4441 ggcgcacaag ccaacagcgc ggagggctgg acgctgatca tgcctgactg gcagaacggg
     4501 gaaggggcaa gcaatcactg gcgattgtcg gtggtggtgg aatataacca ggggcagcgt
     4561 gtctcctcca atgagatcac gctaacgctt gtcgaaccgt tcgatgcatt gtcaaacgac
     4621 gaactgcgct gggaaccgta atcagaaaat gcgctcctga tgcacccata ccgctgcttc
     4681 cacgcgagac ttgagcttca ttttcttcag catgtgcttg acgtgcactt ttactgtgct
     4741 ttcggtgata tccaggcggc gggcaatcat cttgttcggc aaaccctggg caatcagctt
     4801 gagaatatcg cgctcgcgtg gggttaactg gttaacatcg cgctcagtag tggcacggtt
     4861 agcgcgcaag ctggcggcca gaacaggcgt taatgcttcg cttaatacca tttcgccagc
     4921 agctgcctga tgcaatgctt tcagcagatc ttccggttcc atatctttta acagataacc
     4981 atccgcgccg cgtttcagtg cggtgaccac atcttcttca tggttagaga cgctgaatac
     5041 cacaatgcgc cctgagaggg acttttcgcg cagtttatcc agcgtttcca gaccgttcat
     5101 gccgggcata ttgagatcta acaggatcag atcgggatca agagactccg ccagttcaat
     5161 accctgttcg ccattactcg cttcgccaac cacggtgata tctggtgcca tactgataag
     5221 ctgttttacg ccagttcgca gcatcgggtg atcgtcgatc agcaggatag tagccggttc
     5281 ctgattactc atgggtatct ccttggacgt ctgtgaaagt tttttcggga ataaaggtga
     5341 ccaccacttc ggtgccacct gattcacgac ggcggacgcg gcaatcgcct cgtaaacttt
     5401 gcgcacggtc gcgcattatt atcatgccgt agtgattgct gcggatggca ttttcaggca
     5461 cgccgcagcc gttatcttgg acggtcagtt tgacttgatt atcgttttgc gccaccgtca
     5521 ccacgacctc actcgcttgc gaatgtttga gggcgttact taatgcctca cgggcaattt
     5581 gcaacaagtg gattgcctga tgtgaaggca ccagacgagg cggcaattga taatccagct
     5641 tcaccgggaa gccaaatttg gcgctgtact cttcgcaact cgcctccagc gccggacgta
     5701 atccaggctc ggtgagctgc aagcggaatg tggtgagcaa ttcacgcaac tgcgcccagg
     5761 atgcattcag ttcgttacgg atctgactta acagttcgcg gctgcttgct ggcagcgcat
     5821 cgccctgcat ctgtaaacaa ctcacctgca tcttcatgca agagagagat tgggcaatag
     5881 aatcatgcag ttcgcgcgca atggtggcac gctcttccat cacgatcaac tgttgctgac
     5941 gttcctgatg gcgatccagc gccagcgtgg cggtgagttg ttcaaccagg gtatccacca
     6001 gttgttgttg atcatggcta agatgacgcc cctgcggcag ggtcgccagc aaaataccgt
     6061 actgcgtatg agagtcagcc agccgccact tcagggttgt gccgcgatca ccaacgggta
     6121 atacgccgcg cgggcagagc tggcagcctt tatcatcaca agtcatatct ggctggcagg
     6181 taaactcctg atgattctct tcatcatcag tgtcatacac ccgcagttcg atatcacgta
     6241 gcagggttaa attctgtaag ccgttgagta caggtgacag gcgttcacac agcggggcgc
     6301 gggaatgcaa acggcggtta gcctgccata aaaaagagag gatctgattt ttatgctcca
     6361 gcccggcggt tttctcctga acccgctgct caagtacggc ataactttcg gccagttctg
     6421 cagacatatt gttcaatgca gttccaagca tcgccatttc gttgcgcccg ctgatgttcg
     6481 cgcgttgggt aaaatcgcga tgactgacgg cactcgccat tgccagcagt tgccgccacg
     6541 gttgtagcag tcgcacccgc aaccagataa tagtgaacac cagtaaaagt gccataaata
     6601 ccgccattac ccgatggacc agtaccactg tctcaatgcg catttccgtg gtgcggtcaa
     6661 aaccagatac cagctgatca agcccggcaa caaactggct gacatccgct gacaccgttt
     6721 cgcggttttg tgcacgcatc agtgcaggga tcagttcatt acgccagtaa tcttgtaaac
     6781 cctgtaattg cgccagttgt tcgtctcgtt ctgctgctcg agttaactcg gcgctaaatg
     6841 ccgtttgttc catctcttta attaagggct tgtctttctc acttaatggc actgccgcca
     6901 acagacggta actttgcatg cgcagcgatc ccgctttgtt gatcgcatgg gcgctgccct
     6961 gaacgccttg caccagccag ccagaaaccg ccatccctgc cagtccaata gcagtagaaa
     7021 gcaacacaat aagcgcaacc tgattaacca gggtgagcgg agagagacaa cgtttaagca
     7081 tgtaaacctc ttccttcagg ctttaaatga gcaataacct taatgaatgt gacgatacat
     7141 tctggaatgg cagtattctc ggctattggc tgaagtatac ccatacccgg aaagagttac
     7201 tccttatttg ccgtgtggtt agtcgcttta catcggtaag ggtagggatt ttacagcacc
     7261 gtgaaaaatc tcataatttt tatgaaatca ctgtactcac tatgggtaat gataaatatc
     7321 aatgatagat aaagttatct tatcgtttga tttacatcaa attgccttta gctacagaca
     7381 ctaaggtggc agacatcgaa acgagtatca gaggtgtcta tgagtcactc atccgccccc
     7441 gaaagggcta ctggagctgt cattacagat tggcgaccgg aagatcctgc gttctggcaa
     7501 caacgcggtc aacgtattgc cagccgtaac ctgtggattt ccgttccctg tctgctgctg
     7561 gcgttttgcg tatggatgtt gttcagcgct gttgcggtga acttaccgaa agtcggtttt
     7621 aattttacga ccgatcagct atttatgttg actgcgctgc cttcggtttc tggcgcgtta
     7681 ttacgtgttc catactcctt tatggttcct atcttcggtg gtcgtcgctg gacggcgttc
     7741 agcaccggta ttctgattat tccttgcgtc tggctgggtt ttgccgtgca ggatacctcc
     7801 acgccttata gcgtcttcat catcatctct ctgctatgcg gctttgctgg cgcgaacttc
     7861 gcatccagta tggcaaacat cagcttcttc tttccgaaac agaagcaggg tggcgcgctg
     7921 ggtctgaatg gtggtctggg aaacatgggc gtcagcgtca tgcagttggt tgctccgctg
     7981 gtggtatcac tgtcgatttt cgcagtattt ggtagccagg gcgtcaaaca gccggatggg
     8041 actgagctgt atctggcgaa tgcgtcctgg atatgggtgc cgttccttgc catcttcacc
     8101 attgcggcgt ggtttggcat gaacgatctt gctacctcga aagcctccat caaggagcag
     8161 ttgccggtac tcaaacgggg tcatctgtgg attatgagcc tgctgtatct ggcaaccttc
     8221 ggctccttca tcggcttctc cgcgggcttt gcaatgctgt caaaaacgca gttcccggat
     8281 gttcagattc tgcaatacgc tttcttcggg ccgtttattg gtgcgctggc gcgttctgca
     8341 ggtggtgcat tatctgaccg tctgggcgga actcgtgtca cgctggtgaa ctttattctg
     8401 atggcgattt tcagcggcct gctgttcctg accttaccga ctgacgggca gggcggaagc
     8461 ttcatggcgt tcttcgcggt cttcctggcg ctgttcctga cagctgggct gggtagtggt
     8521 tccactttcc agatgatttc agtgatcttc cgtaaactga caatggatcg cgtgaaagca
     8581 gaagggggtt ctgacgaacg tgcgatgcgt gaagcggcaa ccgacacggc ggcggcgctg
     8641 ggtttcatct ctgcgattgg cgcgattggt ggcttcttta tcccgaaagc gtttggtagc
     8701 tcgctggcat taacgggttc gccagtcggc gcaatgaagg tatttttgat tttctatatc
     8761 gcctgcgtag tgattacctg ggcggtatat ggtcggcatt ctaaaaaata aaccgttact
     8821 cgtcatactc cgggttacat gtgctgcggc tgcgttcatt caccccagtc acttacttta
     8881 gtaagctcct gggattcatt cacttgccgc cttcctgtaa accgaattat atagagtaaa
     8941 atatttgatt atcctttgcg cggcatgatg tcgcgctttt tttaagcgtt atttagttac
     9001 aacatactaa tgttatatgg tttatttcgc cggatttcat taagagccat taatatgtta
     9061 cccatgggga atactcctta atacccatct gcataaaaat cttaatagtt taaataacta
     9121 caggtataaa acgtcttaat ttacagtctg ttatgtggtg gctgttgatt atcctaaagg
     9181 ggtatcttag gaatttactt tatttttcat ccccatcact cttgatcgtt atcaattccc
     9241 acgctgtttc agagcgttac cttgccctta aacattagca atgtcgattt atcagagggc
     9301 cgacaggctc ccacaggaga aaaccgatga gtaaattcct ggaccggttt cgctacttca
     9361 aacagaaggg tgaaaccttt gccgatgggc atggccagct tctcaatacc aaccgtgact
     9421 gggaggatgg atatcgccag cgttggcagc atgacaaaat cgtccgctct acccacgggg
     9481 taaactgcac cggctcctgc agctggaaaa tctacgtcaa aaacggtctg gtcacctggg
     9541 aaacccagca gactgactat ccgcgtaccc gtccggatct gccaaaccat gaacctcgcg
     9601 gctgcccgcg cggtgccagc tactcctggt atctttacag tgccaaccgc ctgaaatacc
     9661 cgatgatgcg caaacgcctg atgaaaatgt ggcgtgaagc gaaggcgctg catagcgatc
     9721 cggttgaggc atgggcttct atcattgaag acgccgataa agcgaaaagc tttaagcagg
     9781 cgcgtggacg cggtggattt gttcgttctt cctggcagga ggtgaacgaa ctgatcgccg
     9841 catctaacgt ttacaccatc aaaaactacg gcccggaccg tgttgctggt ttctcgccaa
     9901 ttccggcaat gtcgatggtt tcttacgcat cgggtgcacg ctatctctcg ctgattggcg
     9961 gtacttgctt aagcttctac gactggtact gcgacctgcc tcctgcgtct ccgcaaacct
    10021 ggggcgagca aactgacgta ccggaatctg ctgactggta taactccagc tacatcatcg
    10081 cctggggttc aaacgtgccg cagacgcgta ccccggatgc tcacttcttt actgaagtgc
    10141 gttacaaagg caccaaaact gttgccgtca caccagacta cgctgaaatc gccaaactgt
    10201 gcgatctgtg gctggcaccg aaacagggca ccgatgcggc aatggcgctg gcgatgggcc
    10261 acgtaatgct gcgtgaattc cacctcgaca acccaagcca gtatttcacc gactatgtgc
    10321 gtcgctacac cgacatgccg atgctggtga tgctggaaga acgcgacggt tactacgctg
    10381 caggtcgtat gctgcgcgct gctgatctgg ttgatgcgct gggccaggaa aacaatccgg
    10441 aatggaaaac tgtcgccttt aataccaatg gcgaaatggt tgcgccgaac ggttctattg
    10501 gcttccgctg gggcgagaag ggcaaatgga atcttgagca gcgcgacggc aaaactggcg
    10561 aagaaaccga gctgcaactg agcctgctgg gtagccaaga tgagatcgct gaggtaggct
    10621 tcccgtactt tggtggcgac ggcacggaac acttcaacaa agtggaactg gaaaacgtgc
    10681 tgctgcacaa actgccggtg aaacgcctgc aactggctga tggcagcacc gccctggtga
    10741 ccaccgttta tgatctgacg ctggcaaact acggtctgga acgtggcctg aacgacgtta
    10801 actgtgcaac cagctatgac gatgtgaaag cttatacccc ggcctgggcc gagcagatta
    10861 ccggcgtttc tcgcagccag attattcgca tcgcccgtga atttgccgat aacgctgata
    10921 aaacgcacgg tcgttcgatg attatcgtcg gtgcggggct gaaccactgg tatcacctcg
    10981 atatgaacta tcgtggtctg atcaacatgc tgattttctg cggctgtgtc ggtcagagcg
    11041 ggggcggctg ggcgcactat gtaggtcagg aaaaactgcg tccgcaaacc ggctggcagc
    11101 cgctggcgtt tgcccttgac tggcagcgtc cggcgcgtca catgaacagc acttcttatt
    11161 tctataacca ctccagccag tggcgttatg aaaccgtcac ggcggaagag ttgctgtcac
    11221 cgatggcgga caaatcccgc tataccggac acttgatcga ctttaacgtc cgtgcggaac
    11281 gcatgggctg gctgccgtct gcaccgcagt taggcactaa cccgctgact atcgctggcg
    11341 aagcggaaaa agccgggatg aatccggtgg actatacggt gaaatccctg aaagagggtt
    11401 ccatccgttt tgcggcagaa caaccagaaa acggtaaaaa ccacccgcgc aacctgttca
    11461 tctggcgttc taacctgctc ggttcttccg gtaaaggtca tgagtttatg ctcaagtacc
    11521 tgctggggac ggagcacggt atccagggta aagatctggg gcaacagggc ggcgtgaagc
    11581 cggaagaagt ggactggcag gacaatggtc tggaaggcaa gctggatctg gtggttacgc
    11641 tggacttccg tctgtcgagc acctgtctct attccgacat cattttgccg acggcgacct
    11701 ggtacgaaaa agacgacatg aatacttcgg atatgcatcc gtttattcac ccgctgtctg
    11761 cggcggtcga tccggcctgg gaagcgaaaa gcgactggga aatctacaaa gccatcgcga
    11821 agaaattctc cgaagtgtgc gtcggccatc tgggtaaaga aaccgacatc gtcacgctgc
    11881 ctatccagca tgactctgcc gctgaactgg cgcagccgct ggatgtgaaa gactggaaaa
    11941 aaggcgagtg cgacctgatc ccaggtaaaa ccgcgccaca cattatggtc gtagagcgcg
    12001 cttatccggc gacttacgaa cgctttacct ctatcggccc gctgatggag aaaatcggta
    12061 atggcggtaa agggattgcc tggaacaccc agagcgagat ggatctgctg cgtaagctca
    12121 actacaccaa agcggaaggt ccggcgaaag gccagccgat gctgaacacc gcaattgatg
    12181 cggcagagat gatcctgaca ctggcaccgg aaaccaacgg tcaggtagcc gtgaaagcct
    12241 gggctgccct gagcgaattt accggtcgtg accatacgca tctggcgctg aataaagaag
    12301 acgagaagat ccgcttccgc gatattcagg cacagccgcg caaaattatc tccagcccga
    12361 cctggtctgg tctggaagat gaacacgttt cttacaacgc cggttacacc aacgttcacg
    12421 agctgatccc atggcgtacg ctctctggtc gtcagcaact gtatcaggat caccagtgga
    12481 tgcgtgattt cggtgaaagc ctgctggttt atcgtccgcc gatcgacacc cgttcggtga
    12541 aagaagtgat aggccagaaa tccaacggca acccggaaaa agcgctcaac ttcctgacgc
    12601 cgcaccagaa gtgggggatc cactccacct acagcgacaa ccagttgatg ctgaccttag
    12661 gtcgcggtgg cccggtggtc tggctgagcg aagccgatgc caaagagctg ggcattgccg
    12721 ataacgactg gattgaagtt tacaacagca acggtgcgct gactgcccgt gcggttgtca
    12781 gccagcgtgt tccggcaggg atgaccatga tgtaccacgc gcaggaacgt atcgttaacc
    12841 tgccgggttc ggaaattact ggccagcgtg gggggatcca caactcggtc acccgtatca
    12901 cgccgaaacc gacgcatatg attggtggct atgcccatct ggcatacggc tttaactact
    12961 acggcaccgt aggttctaac cgcgatgagt ttgttgtagt gcgtaagatg aagaacattg
    13021 actggttaga tggcgaaggc aatgactagg tacaggagag cgtaaaatga aaattcgttc
    13081 acaagtcggc atggtgctga atctcgataa gtgcatcggc tgccacacct gttcagttac
    13141 ctgtaaaaac gtctggacca gccgtgaagg cgtggagtac gcgtggttca acaacgtgga
    13201 aaccaagccg ggtcagggct tcccgactga ctgggaaaac caggaaaaat acaaaggcgg
    13261 ctggatccgt aaaatcaacg gcaaactgca gccgcgcatg ggtaaccgtg ccatgttgct
    13321 gggtaaaatc ttcgctaacc cgcatctgcc ggggatcgac gattattacg agccgttcga
    13381 ttttgactat cagaacctgc ataccgcgcc ggaaggcagc aaatcgcagc cgattgcccg
    13441 tccccgttcg ctgattaccg gggaacggat ggcgaaaatc gaaaaagggc cgaactggga
    13501 agatgacctg ggtggtgagt ttgacaaact ggcgaaagac aagaacttcg acaacatcca
    13561 gaaggcgatg tatagccagt tcgaaaacac ctttatgatg tatttgccgc gcctgtgcga
    13621 acactgcctg aacccggcat gtgtggcgac ctgcccgagc ggtgcgattt acaagcgtga
    13681 agaagatggc atcgtcctga tcgaccagga taaatgccgt ggctggcgta tgtgcatcac
    13741 cggatgcccg tacaaaaaaa tctacttcaa ctggaagagc ggtaagtctg agaagtgcat
    13801 cttctgctat ccgcgtattg aagcgggtca gccgaccgtg tgctcagaaa cctgtgtcgg
    13861 tcgtatccgt tatcttggcg tgctgctgta cgatgccgac gcgattgaac gtgcagccag
    13921 caccgagaac gagaaagatc tttaccagcg tcagctggag gtgttcctcg atccgaacga
    13981 tccgaaagtc atcgagcagg cgattaaaga cggtattccg ctgagcgtta ttgaagccgc
    14041 acagcagtcg ccggtttata aaatggcaat ggaatggaaa ctggcgctgc cgctgcatcc
    14101 agaatatcgc acactgccga tggtctggta cgtgccgcct ctgtctccga ttcagtctgc
    14161 agcagacgcg ggtgagctgg gtagcaacgg cattctgcca gacgtcgaaa gcttgcgtat
    14221 tccggtacag tatctggcga atctgctgac cgccggtgat accaaaccgg tactgcgcgc
    14281 actgaaacgt atgctggcga tgcgtcatta caaacgtgct gaaaccgttg acggtaaagt
    14341 tgatacccgt gcgctggaag aggtcggtct gaccgaagcc caggcacagg agatgtaccg
    14401 ttatctggct attgctaact acgaagatcg ctttgtggtg ccgagtagtc atcgtgaact
    14461 ggcacgggaa gccttcccgg agaaaaatgg ctgcggcttt acctttggtg atggctgcca
    14521 cggctcagat accaaattca atctgttcaa cagccgtcgt atcgatgcca tcgatgtgac
    14581 cagcaaaacg gagccgcatc catgatcgaa ctcgtgattg tatcgcgtct ccttgaatat
    14641 ccggatgctg ccttatggca gcatcaacaa gagatgtttg aggtgattgc tgcgtcgaaa
    14701 aatctgtcga aagaagatgc ccatgcgctg ggcattttcc tgcgcgattt aacggcgatg
    14761 gatccgctcg atgcccaggc ccagtacagc gaactgttcg accgtggccg cgccacgtca
    14821 ctgttgctat ttgaacatgt tcacggtgaa tcccgtgacc gcggccaggc gatggtggac
    14881 ctgctggcgc agtacgagca gcacggcttg cagttaaaca gccgcgaatt accggaccat
    14941 ctgccgctgt atctggagta cctgtcgcag ctgccgcaaa gcgaagccgt ggaaggtttg
    15001 aaagatatcg cgccgattct ggcattgctg agcgcgcgtc tgcaacagcg tgaaagccgt
    15061 tatgccgtga tgtttgatct gctgctgaaa ttggccaata ccgctatcga cagcgacaaa
    15121 gtggcggaaa aaattgccga cgaagcgcgc gatgatacgc cgcaggcgct ggatgctgtc
    15181 tgggaagaag agcaggttaa attctttgct gacaaaggct gcggtgattc agcaatcact
    15241 gctcatcagc gtcgctttgc cggtgccgtc gcgccgcaat atctgaatat caccaccgga
    15301 ggacagcact aatgcaattc ctgaatatgt tcttctttga tatctacccg tacatagccg
    15361 gggcggtctt cctgattggt agctggctgc gttatgacta cgggcagtac acctggcgcg
    15421 cggcgtccag ccagatgctg gatcgcaaag ggatgaacct ggcgtcgaac ctgttccata
    15481 tcggtattct ggggattttt gctggtcact tcttcggtat gctgacgccg cactggatgt
    15541 atgaagcctg gctgccgatt gaagtgaaac agaaaatggc gatgtttgct ggtggtgcca
    15601 gcggcgtgct gtgtctgatt ggcggcgtgc tgttgctgaa gcgtcgtctg ttcagcccac
    15661 gcgtgcgtgc aaccactacc ggagcggata tcctgatcct gtcgctgctc gttatccagt
    15721 gcgcgctggg cctgttgacc attccgttct ccgctcagca tatggacggt agcgagatga
    15781 tgaaactggt tggctgggcg cagtcggtgg tgaccttcca cggtggcgct tctcaacacc
    15841 tcgatggtgt ggcgtttatc ttccgtctgc acctggtgct ggggatgacg ttattcctgc
    15901 tgttcccgtt ctcgcgtctg gtacacatct ggagcgtacc ggtggagtat ctgacacgta
    15961 agtaccagct ggtgcgcgct cgtcactaaa cgaattttag ttcacataga ccctgcttcg
    16021 gcggggtttt ttatgggcac ggtgcggggt gagttgtcgg atgcgcttcg cttatccgac
    16081 ctacagggga ggatattgta ggcccggtaa acgtggtgcc atcgggcaaa attagctcgg
    16141 agtaacaggt tttgattatt caaggaggcg atggtggtgg gggaaggatt actcagcgct
    16201 gcgcgcttcg cccttcgggt cgttgcctgc ggcaacgctc tctcgctggc gctcaagtcg
    16261 aaccttggtc gaagcttctc atccttcccc gcttgggcag aatatttaat tgcggattcg
    16321 tttgagagtt tacggacttt tgaaaagtga tggtggtggg ggaaggattc gaaccttcga
    16381 agtcgatgac ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccagggg
    16441 taattcaaat tttgaggtaa tgcttgagat ggtggtgggg gaaggattcg aaccttcgaa
    16501 gtcgatgacg gcagatttac agtctgctcc ctttggccgc tcgggaaccc caccacgggg
    16561 taatgctttt tactggcctg ctcccttatc gggaagcggg gcgcatcata tcaaatgacg
    16621 cgccgctgta aagtgttacg ttgagaaaaa tgaactggtt gcgtaatttt catccgtaac
    16681 ggattaaagg taaccagtta tttttgctgg cgattaaaga ataatcgtcc gattaccgta
    16741 aacaaagacg cgctgtgcca gtactttgta tagcgcacga cttaagacgt ttttctcgac
    16801 gtcacgacct gcgcgcatca tatcttcagc tgtgtaggta tgatcgacat gaataacgtc
    16861 ctgcatgatg attgggcctt cgtccagatt gtcattcaca tagtgagcgg ttgcgccaat
    16921 aatcttcaca ccacgttcat aggcctggtg ataaggacgt gcgccgataa acgcaggcag
    16981 gaatgaatgg tgaatattga tgattttatt cgggaagcgt gccacaaatt ccggcgttaa
    17041 tacccgcata tacttcgcca gcaccacata gtcaggttta taagcatcaa tggcgtctgc
    17101 catcttttga tcgtgctcgt tgcggcttaa cccttcatgg tttaccagct caaacggaat
    17161 atcaaaacgc tcaaccagag aacgtaaagt atcgtggtta ccaataactg ccgcgatttc
    17221 gacatccagg ccgccgtaat tggctttcat caacaaatcg ccaaggcaat gcgcttcttt
    17281 agtgaccaga atcactatcc ggcgacgacc ggcaggattc agctcacgca cggagccttc
    17341 tggcaatgcg ctatcgagat ccgccagcag ggtggaatca ttaaaaatcc cttccagttc
    17401 cgtgcgcata aaaaagcgcc cggtacggtg atcaacaaat tcattgttct gtacgatatt
    17461 taactcgtgc ttgtagcaaa tattggtaat acgtgcgatc agaccttttt ggtccggaca
    17521 aatagtacgc agaactttac gttggagtga atgcattgct ggaaaaacct tgttgagagt
    17581 gtttgctaaa ccgtaccgtc aaccattatt ggccgcagca ctttttaaat tttttacctg
    17641 aaccacaagg gcagggatcg ttgcgaccaa actgcggacg tgtaccgtca atatagtacc
    17701 attgaccgtt ttctttcaaa aatcgcgaac gttcaattat tgcacctgtt ttgccacctt
    17761 cggtaaatcg agcaacaaaa ctgacaaatc cgatattgtc ggcgtcctgc caacaatgtt
    17821 caaatacggt caatcccaac cattcagtat gcgcaaatcc ggccatcaat tcggcacgta
    17881 acgccgctgc tccacaagag ggatgccagg tctttattaa ataatctgcg tcttgcatca
    17941 caaaagcgca gtaacgcgaa cgcatgagat gttctggatc aggtgcaacc ttttcaccag
    18001 acacataagg gtggcaacat aggctatact cgacagcact accacaggga caaagctgag
    18061 acacaaataa tctccctgga aacaataacg gcgtattaac cgcctgagta gcactatgtt
    18121 aaccgagcag tagcgatgtg gctacgattg cattccaggg gaatcttgcg ggaataatga
    18181 gaaagataaa aatagggctg gcgctgggat ctggcgcggc gagaggttgg tcgcatattg
    18241 gcgttattaa tgcgctaaaa aaagtgggta ttgaaattga tatcgttgca ggatgttcaa
    18301 ttggttcgct ggtgggcgct gcctatgcat gcgatcgatt atctgcgctg gaagattggg
    18361 tgacctcttt cagttattgg gatgttttac gcctgatgga tctctcctgg cagcgcggtg
    18421 ggttactgcg cggcgagcgt gtcttcaatc aatgccgcga aataatgccg gaaacagaga
    18481 tcgaaaattg ttcccgtcgc tttgcggctg ttgccaccaa tttaagtacg ggacgtgaat
    18541 tatggtttac tgaaggcgat ctccatcttg ctattcgcgc atcatgcagt attccaggac
    18601 tcatggcacc tgttgcacat aacggctact ggctggttga tggagcagta gttaacccaa
    18661 ttcctatttc cctcacgcgt gtattggggg ctgatattgt gatagcggtc gacctgcagc
    18721 acgatgctca tttgatacaa caagatttgc tctcctttaa tgtcagtgaa gaaaatagcg
    18781 agaatggtga atctttgccg tggcatgcgc gtctgaaaga aaggttaggc agtataacaa
    18841 cacgtcgggc ggtgacagcg ccaacggcaa cagagattat gaccacttct atccaggtgc
    18901 tggagaaccg ccttaaaagg aaccgcatgg caggtgatcc gcccgatatt ctgattcaac
    18961 ctgtttgccc gcaaatatct acgcttgatt tccatcgcgc gcacgctgcc attgcggccg
    19021 gacagctggc agtggaaagg aaaatggacg aacttttgcc gttggtacgc accaacattt
    19081 gaccagaatt tttatctaca cttaagttaa ttctgacagg cgcaggtggc aatagcatgc
    19141 cactattgag taaagccagt caggggagag aacatgacgc agccattggt cggaaaacag
    19201 attctcattg ttgaagatga gcaggtattt cgctcgcttc tggattcatg gttttcctca
    19261 ttgggagcga caacggtact ggcggctgat ggggtggatg cccttgagtt gctgggaggt
    19321 ttcactccag acctgatgat atgtgatatc gcgatgccac gaatgaacgg gcttaaactg
    19381 ctggagcata tacgtaacag aggcgaccag accccagttc tggtgatatc tgccactgaa
    19441 aatatggcag atattgccaa agcgttacgt ctgggcgttg aagatgtttt gctgaaacca
    19501 gttaaagatc tgaatcgctt gcgcgagatg gtttttgcct gtctctatcc cagcatgttt
    19561 aattcgcgcg ttgaggaaga ggaaaggctt tttcgcgatt gggatgcaac ggttgataac
    19621 cctgccgcag cggcgaaatt attacaggaa ctacaaccgc cggttcagca ggtgatttcc
    19681 cattgccggg ttaattatcg tcaattggtt gccgcggaca aacccggcct ggtgcttgat
    19741 attgccgcac tttcggaaaa cgatctggca ttttattgcc ttgatgtcac ccgagctgga
    19801 cataatggcg tacttgctgc cttgttatta cgcgcattgt ttaacggatt attacaggaa
    19861 cagcttgcac accaaaatca acggttgcca gagctgggcg cgttattgaa gcaggtaaac
    19921 catttactcc gtcaggccaa tctgccgggg cagtttccgc tattagttgg ctattatcat
    19981 cgcgaactga aaaatctcat tctggtttct gcgggtctga atgcgacgtt aaataccggc
    20041 gaacaccagg tgcaaatcag taatggtgtt ccgttaggca ctttaggtaa cgcttatttg
    20101 aatcaattga gccagcgatg cgatgcctgg caatgccaaa tatggggaac cggtggtcga
    20161 ctgcgcttga tgttgtctgc agaatgagca aacgataacg cggactaaat ttgcattacc
    20221 tgctaatgtc ggctggtggt actatcgtcg ccattcgtat aagtaattgt cttaattatg
    20281 ctaactcgcc tccttttcag aacttagccc cttcggggtg ctgatatact gggatgcgat
    20341 acagaaatat gaacacgttc aaaacacgaa cagtccagga gaatttaaat ggctgccatt
    20401 aatacgaaag tcaaaaaagc cgttatcccc gttgcgggat taggaaccag gatgttgccg
    20461 gcgacgaaag ccatcccgaa agagatgctg ccacttgtcg ataagccatt aattcaatac
    20521 gtcgtgaatg aatgtattgc ggctggcatt actgaaattg tgctggttac gcactcatct
    20581 aaaaactcga ttgaaaacca ctttgatacc agttttgaac tggaagcaat gctggaaaaa
    20641 cgtgtaaaac gtcaactgct tgatgaagtg cagtctattt gtccgccgca cgtgactatt
    20701 atgcaagttc gtcaggggct ggcgaaaggc ctgggacacg cggtattgtg tgctcacccg
    20761 gtagtgggtg atgaaccggt agctgttatt ttgcctgatg ttattctgga tgaatatgaa
    20821 tccgatttgt cacaggataa cctggcagag atgatccgcc gctttgatga aacgggtcat
    20881 agccagatca tggttgaaca ggttgctgat gtgaccgcat atggcgttgt ggattgcaaa
    20941 ggcgttgaat tagcgccggg tgaaagtgta ccgatggttg gtgtggtaga aaaaccgaaa
    21001 gcggatgttg cgccgtctaa tctcgctatt gtgggtcgtt acgtacttag cgcggatatt
    21061 tggccgttgc tggcaaaaac ccctccggga gctggtgatg aaattcagct caccgacgca
    21121 attgatatgc tgatcgaaaa agaaacggtg gaagcctatc atatgaaagg gaagagccat
    21181 gactgcggta ataaattagg ttacatgcag gccttcgttg aatacggtat tcgtcataac
    21241 acccttggca cggaatttaa agcctggctt gaagaagaga tgggcattaa gaagtaacat
    21301 ccatatcggt gttatccacg aaacggcgtt gaacaatcga cgccgttttt ttatagctta
    21361 ttcttattaa attgtcttaa accggacaat aaaaaatccc gccgatggcg ggatttttag
    21421 caattgcaat ctacaaaaga ttattgcttg atcaggaaat cgtcgaggga tttaccttgc
    21481 tcatccattg cttttttgat tacagctgga gtacggccct ggccagtcca ggttttagtt
    21541 tcgccgtttt cgtcaacata gctatatttt gccggacgct gagcacgttt agctttggtg
    21601 ccggatttaa cggcagcaag gctattcagc agttcgttcg ggtcaatacc gtcagcgatc
    21661 agcatttcgc gatattgctg cagtttacga gtgcgctctt caacttcagc agcagccgcg
    21721 ctttcttctt cgcgacgttc gttaacaaca acttctaatt tttccagcat ttcttccagc
    21781 gtttcaagtg tacattctct tgcctgcgca cgaagagtac ggatgttgtt cagaatttta
    21841 agtgcttcgc tcattgtagt aatctcaaac ttatattggg gtggtttgtt gaggtaataa
    21901 tagagcctta aattcagttg tgcaatagcc aggaatgtaa ggaattcaaa attgttcttt
    21961 attttgtgcc gccaataaat atcttttcat aaaattagcc agaaaagacg cggcatatag
    22021 ccctatttac accgatgatt tcgcagcacg tgaggttaaa acttcctgat tcatgtcaca
    22081 ttttatgggg agattatcgt aggctgacgg cctttcagtc ttctgtatta gttgtgttta
    22141 cgagaattcc ctattaagcg aatgatgaaa agtagaacag tcgcaataag agcatggact
    22201 tagtattgca ctatctcctg gaggtcaaca gagggctatt acttgcgcaa caggttaaag
    22261 attgtgaata gttaccagca gtcatttacc cgcttataac aagcgaggca gttgtaatga
    22321 tagctcagaa ggattatgca aggcttcgta agggagaacg catataccca cttctgtgca
    22381 tactgttgag ctgaaaaact gacgaattat gataaactcc agccaacttt atttcatatc
    22441 attgagggcc tgtggctgat ggcacagcta tatttctact attccgcaat gaatgcgggt
    22501 aagtctacag cattgttgca atcttcatac aattaccaag aacgcggcat gcgcactgtc
    22561 gtatatacgg cagaaattga tgatcgcttt ggtgccggga aagtcagttc gcgtataggt
    22621 ttgtcatcgc ctgcaaaatt atttaaccaa aattcatcat tatttgatga gattcgtgcg
    22681 gaacatgaac agcaggcaat tcattgcgta ctggttgatg aatgccagtt tttaaccaga
    22741 caacaagtat atgaattatc ggaggttgtc gatcaactcg atatacccgt actttgttat
    22801 ggtttacgta ccgattttcg aggtgaatta tttattggca gccaatactt actggcatgg
    22861 tccgacaaac tggttgaatt aaaaaccatc tgtttttgtg gccgtaaagc aagcatggtg
    22921 ctgcgtcttg atcaagcagg cagaccttat aacgaaggtg agcaggtggt aattggtggt
    22981 aatgaacgat acgtttctgt atgccgtaaa cactataaag aggcgttaca agtcggctca
    23041 ttaacggcta ttcaggaaag gcatcgccac gattaataag aatttcttta ctgacagggt
    23101 gagcagggca cttttatcct gtcagttcgt tttacgcact tcttccgggc tatataccct
    23161 tctcggcagt tttttaacgc cgctatacgc ctcacagggc tcttaagcac cgacgttgac
    23221 ttgtgacctg taaagtacaa tatccctgtg tttaggcgtt atacatcgtc gcaaatatga
    23281 tgaaggctaa tgctgtcggt ttatggaaaa gttgctttgg gtaaacaaaa aatacggccc
    23341 cagaagggca atgccgttca cttaagagga gcggcactat gtttcacagg ataacgggtt
    23401 tttgatatct taaccgacct cggccttgat ggtcgggggc gttttgttat gaacaccact
    23461 tccagagcac cccgaagatg ctccagtcgt ttcgggatgg tccctggtga cgccgtgttt
    23521 cccagctcta tcatttctga tgcgatattc ttccacgcag gcagtagcca gtggcggtta
    23581 caacccttct ggttcagcgt tagcagcagg tcttcgctgt aaaaaagctt atcaaacaac
    23641 gtaatagagt tatccgggat ggtggcgagc atggagtggg ccagcacagt ttcgctctgc
    23701 cggtaaggtg cggtcacggc attcagcaga atgtgacttc ccaggttcat taaggccacc
    23761 agacgcataa ccgggtaggc gttctgccgc ttagtggatg tgttggcaga cccataatat
    23821 tcacgcagct cgggtttatc aggtgtcctg aactgtgcgc catcaatggc aaaaagttgc
    23881 aggccgtgcc agtcatcctt caggtaacgt tccgcgcccc tgtctgtgcg gtctggcgga
    23941 agagccattc cactggggcg gcccccacgc gctgacgcgc ttgggtgaca gcgctgcggg
    24001 ccagcaggtt catccccgct tcgccatccg cgctcaggtt cagacggcga acaacatcgg
    24061 taattggctc attgcaccac catccagata accatgtccc ccggtaaacg acggcggcga
    24121 acggtcgcat gagcagaaag cgtcaggcag tgttgtatcc actcggtggg aaggtgttct
    24181 gcaaatagtt gtgcagaggg cggaggcata agcggatggt cactgaaatc gagcagatca
    24241 ttgagaagtg gcataagaaa acggctccct gttgtggaag ccgttatagt gcctcagttt
    24301 aaggatcggt caactaatcc ttaactgatc ggcattgccc agaaggggcc gtttatgttg
    24361 ccagacagcg ctactgatta agcggatttt ttcgcttttt tctcagcttt agccggagca
    24421 gcttctttct tcgctgcagt ttcaccttct acataatcac gaccgtagta ggtatccagc
    24481 agaatctgtt tcagctcgga gatcagcggg taacgcgggt tagcgccggt gcactggtca
    24541 tcgaatgcat cttcagacag tttatccacg ttcgccagga agtctgcttc ctgaacgcca
    24601 gcttcacgga tagatttcgg aatacccagt tcagctttca gcgtttccag ccatgccagc
    24661 agtttctcga tcttagcagc agtacggtcg cccggtgcgc tcagacccag gtggtcagca
    24721 atttcagcat aacgacggcg agcctgcgga cggtcatact ggctgaatgc agtctgcttg
    24781 gtcgggttgt cgttcgcgtt gtagcgaata acgttacaaa tcagcagggc gtttgccaga
    24841 ccgtgcggaa tatggaactg ggaacccagt ttgtgcgcca ttgagtgaca tacacccagg
    24901 aaggcgttcg caaacgcgat acccgcgata gtcgctgcac tgtgaacacg ttcacgcgct
    24961 accggatttt tagacccttc gtggtaggac gctggcagat attctttcag cagtttcagt
    25021 gcctgcagag cctgaccatc agagaactca gatgccagta cagaaacata agcttccatg
    25081 gcgtgagtta ctgcgtccag accaccgaaa gcacacaggg acttcggcat gtccataacc
    25141 aggttggcgt cgacaatcgc catatccgga gtcagcgcat agtctgccag cggatatttc
    25201 tgaccagtag cgtcgtcagt tacaaccgca aacggagtga cttcagaacc tgtaccagaa
    25261 gtggtggtga cagcgatcat tttcgctttc acgcccattt tcgggaactt gtagatacgt
    25321 ttacggatat ccataaagcg cagcgccagc tcttcgaagt gagtttccgg atgttcgtac
    25381 ataacccaca tgatcttcgc ggcgtccatc ggggaaccac cacccagcgc gataatcacg
    25441 tctggtttga aggagtttgc cagttctgca cctttacgaa cgatgctcag ggtcgggtcc
    25501 gcttctactt cgaagaagac ttcagtttca acgcctgctg ctttcagtac ggaagtgatc
    25561 tgatcagcat aaccattgtt gaacaggaag cggtcagtca cgatgagcgc acgtttgtgg
    25621 ccatcagtaa tcacttcatc cagcgcgatt ggcagggagc cacggcggaa gtagatagat
    25681 ttcggaagtt tgtgccacaa catgttttca gctcgcttag caacggtttt cttgttgatc
    25741 aggtgtttcg gaccaacgtt ttcagagatg gagttaccac cccaagaacc acaacccaga
    25801 gtcagggaag gtgcgagttt gaagttatac aggtcaccga taccaccctg agacgctggg
    25861 gtgttaatca ggatacgagc cgttttcatt ttctgaccga agtaagaaac gcgagccggt
    25921 tggttatcct ggtcagtgta caggcaagag gtatgaccga taccgcccat agcaaccagt
    25981 ttctctgctt tttctaccgc gtcttcgaaa tctttagcgc ggtacattgc cagagtcggg
    26041 gacagttttt catgtgcgaa cggttcgctt tcatcaacaa cggtcacttc accgatcaga
    26101 atcttggtgt tttctggtac agagaagcct gccagttcag caattttata ggctggctga
    26161 ccaacgatag ccgcgttcag cgcaccgttt ttcaggataa catcctgaac agctttcagc
    26221 tctttaccct gcaacagata gccgccgtgg gttgcaaaac gttcacgtac agcgtcataa
    26281 acagagtcaa caacaacaac agactgttca gaagcacaga ttacgccgtt gtcgaaggtt
    26341 ttggacatca gtacagatgc aactgcacgt ttgatatcag cagtttcatc gataacaact
    26401 ggagtgttgc ccgcgcctac accgatagct ggtttaccgg agctgtatgc ggctttaacc
    26461 atgcccggac caccagtcgc gaggatcagg ttgatgtctg ggtggtgcat cagtgcgtta
    26521 gacagttcaa cagaaggttg atcgatccag ccgatcagat ctttcggagc accggcagcg
    26581 atagcagcct gcagaacgat atcagccgct ttgttggtgg catcttttgc acgcgggtgc
    26641 ggggagaaga taatggcgtt acgggtcttc agactgatca gcgatttgaa gatagcagtt
    26701 gaagtcgggt tagtggtcgg aacgataccg caaataatac cgattggttc agcgatagtg
    26761 atggtaccaa aagtgtcgtc ttcagacaga ataccacagg ttttttcatc tttataggcg
    26821 ttgtagatat attcagaagc aaagtggttt ttgatcactt tatcttcgac gatacccatg
    26881 ccggattcgg caacggccat tttcgcgagt gggattcgag catctgcagc agccagagcg
    26941 gcggcgcgga agattttgtc tacttgctct tgagtgaaac tggcatattc acgctgggct
    27001 ttctttacac gctctacgag tgcgttaagt tcagcgacat tagtaacagc cataatgctc
    27061 tcctgataat gttaaacttt tttagtaaat catctgctcg aatacgagag tatagtcagt
    27121 gcggtgatga tttgcttaac ctatgaaaat caaaagctta ctcgcgctca cactcactgt
    27181 gatttactaa aagagtttaa acattagagt tattatctct aatgcgtcac ttccaggtgg
    27241 cgtaagcaag attactcact tctgggtact gattacgtga tccaaatcaa attttttgca
    27301 aagctgacac ctttcagcat cgcttttcgc cattatagct aacagttaat aaattgtagt
    27361 atgatttggt ggctacatta gcatgttttg cacaactaga taacaataac gaatgatagc
    27421 aattttaagt agttaggagg tgaaaaatgc tgtcaaaagg cgtattgtca gcgcgtcttt
    27481 tcaaccttat ttatggctaa cattatccgg cttttgcttc ggagctaacc gtgattcaga
    27541 ccttttttga ttttcccgtt tacttcaaat ttttcatcgg gttatttgcg ctggtcaacc
    27601 cggtagggat tattcccgtc tttatcagca tgaccagtta tcagacagcg gcagcgcgaa
    27661 acaaaactaa ccttacagcc aacctgtctg tggccattat cttgtggatc tcgctttttc
    27721 tcggcgacac gattctgcaa ctttttggta tatcaattga ttcgttccgt atcgccgggg
    27781 gcatcctggt ggtgacgata gcgatgtcga tgatcagcgg caagcttggc gaggataaac
    27841 agaacaagca agaaaaatca gaaaccgcgg tacgtgaaag cattggtgtg gtgccattgg
    27901 cgttgccgtt gatggcgggg ccaggggcga tcagttctac catcgtctgg ggtacgcgtt
    27961 atcacagcat tagctatctg tttggtttct ttgtggctat tgcattgttc gctttatgtt
    28021 gctggggatt gttccgcatg gcaccttggc tggtacgggt tttacgccag accggcatca
    28081 acgtgattac gcgtattatg gggctattgc tgatggcatt ggggattgaa tttatcgtta
    28141 ctggtattaa ggggattttc cccggcctgc ttaattaatt cctttcaaat gaaacggagc
    28201 tgccatgctc cgtttacttc gtcattattt ttactttgtt cccg
//
LOCUS       DAJVOY010000034        28224 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVOY010000034 DAJVOY010000000
VERSION     DAJVOY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.034"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..778)
                     /locus_tag="OQL90_001320"
                     /pseudo
     CDS             complement(1..778)
                     /locus_tag="OQL90_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
     gene            complement(956..1867)
                     /gene="gsiD"
                     /locus_tag="OQL90_001321"
     CDS             complement(956..1867)
                     /gene="gsiD"
                     /locus_tag="OQL90_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1368152.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1870..2790)
                     /gene="gsiC"
                     /locus_tag="OQL90_001322"
     CDS             complement(1870..2790)
                     /gene="gsiC"
                     /locus_tag="OQL90_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1368153.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2808..4346)
                     /gene="gsiB"
                     /locus_tag="OQL90_001323"
     CDS             complement(2808..4346)
                     /gene="gsiB"
                     /locus_tag="OQL90_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1368154.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4366..6237)
                     /gene="gsiA"
                     /locus_tag="OQL90_001324"
     CDS             complement(4366..6237)
                     /gene="gsiA"
                     /locus_tag="OQL90_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1368155.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6224..7189)
                     /gene="iaaA"
                     /locus_tag="OQL90_001325"
     CDS             complement(6224..7189)
                     /gene="iaaA"
                     /locus_tag="OQL90_001325"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1368156.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7393..8628
                     /gene="moeA"
                     /locus_tag="OQL90_001326"
     CDS             7393..8628
                     /gene="moeA"
                     /locus_tag="OQL90_001326"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1368157.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8628..9377
                     /gene="moeB"
                     /locus_tag="OQL90_001327"
     CDS             8628..9377
                     /gene="moeB"
                     /locus_tag="OQL90_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1368158.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9453..10115)
                     /gene="fsa"
                     /locus_tag="OQL90_001328"
     CDS             complement(9453..10115)
                     /gene="fsa"
                     /locus_tag="OQL90_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1368159.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10246..11145
                     /locus_tag="OQL90_001329"
     CDS             10246..11145
                     /locus_tag="OQL90_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1368160.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            11151..13583
                     /locus_tag="OQL90_001330"
     CDS             11151..13583
                     /locus_tag="OQL90_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1368161.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13729..14544
                     /gene="ybiV"
                     /locus_tag="OQL90_001331"
     CDS             13729..14544
                     /gene="ybiV"
                     /locus_tag="OQL90_001331"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1368162.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14696..15961
                     /locus_tag="OQL90_001332"
     CDS             14696..15961
                     /locus_tag="OQL90_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1368163.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFSREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16202..17794)
                     /locus_tag="OQL90_001333"
     CDS             complement(16202..17794)
                     /locus_tag="OQL90_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1368164.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18013..18933
                     /gene="ldtB"
                     /locus_tag="OQL90_001334"
     CDS             18013..18933
                     /gene="ldtB"
                     /locus_tag="OQL90_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1368165.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18992..20110)
                     /locus_tag="OQL90_001335"
     CDS             complement(18992..20110)
                     /locus_tag="OQL90_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1368166.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(20107..20574)
                     /gene="mntR"
                     /locus_tag="OQL90_001336"
     CDS             complement(20107..20574)
                     /gene="mntR"
                     /locus_tag="OQL90_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1368167.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20760..20888
                     /gene="mntS"
                     /locus_tag="OQL90_001337"
     CDS             20760..20888
                     /gene="mntS"
                     /locus_tag="OQL90_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1368168.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            21160..22743
                     /gene="opgE"
                     /locus_tag="OQL90_001338"
     CDS             21160..22743
                     /gene="opgE"
                     /locus_tag="OQL90_001338"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1368169.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22792..23307)
                     /gene="ompX"
                     /locus_tag="OQL90_001339"
     CDS             complement(22792..23307)
                     /gene="ompX"
                     /locus_tag="OQL90_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1368170.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23360..23425)
                     /locus_tag="OQL90_001340"
     CDS             complement(23360..23425)
                     /locus_tag="OQL90_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368171.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23660..24547
                     /gene="rhtA"
                     /locus_tag="OQL90_001341"
     CDS             23660..24547
                     /gene="rhtA"
                     /locus_tag="OQL90_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1368172.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24846..25349
                     /gene="dps"
                     /locus_tag="OQL90_001342"
                     /gene_synonym="pexB"
     CDS             24846..25349
                     /gene="dps"
                     /locus_tag="OQL90_001342"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1368173.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25753..26499
                     /gene="glnH"
                     /locus_tag="OQL90_001343"
     CDS             25753..26499
                     /gene="glnH"
                     /locus_tag="OQL90_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1368174.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26638..27297
                     /gene="glnP"
                     /locus_tag="OQL90_001344"
     CDS             26638..27297
                     /gene="glnP"
                     /locus_tag="OQL90_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1368175.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27294..28016
                     /gene="glnQ"
                     /locus_tag="OQL90_001345"
     CDS             27294..28016
                     /gene="glnQ"
                     /locus_tag="OQL90_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1368176.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
BASE COUNT         6981 a         7540 c         6970 g         6733 t
ORIGIN      
        1 atgtctttgc aatatactcc gtatcgacag aatcaataat attataatta ccttcgctat
       61 tataaataat cagtttttca cgagcgagtt gattcttggc aatgatctca tgaccttgtt
      121 tcaaaaatag aacttcatca ttagcgctga tttgatcaag atactcgttc aaataccgcc
      181 agtttaaatc gacatgaatt ttatctaccg ctaacaaatc gtgatctttg ccgttgatac
      241 gtagcgtaaa agagatacat tttttacccg gacgatcaaa gcaattgtat gccggattcc
      301 agctgttgtt agagtcatct gaagccaccg accacggtcg tttcgatggg ttaaaatcgg
      361 caaaaataac ccgccctgac tcgtcgacaa gcggtccatt tacttattcc tgatgataaa
      421 caacgatttt atcattcgcc ctccgtgaaa agagataata tttattatca tcaagtacca
      481 gtcccaccga gtctaaaaat ggcatcattt ccattttcag aatcacggca cgttgaatct
      541 cctctgtatt ttttaataaa tacagtgagt cctttaatgt cagggccgaa cgttcaatat
      601 ctttaaagaa atagccaacg tttttctgaa taagttcaaa gcggctatcg gcacgtttta
      661 ccgcgcgttc cgataccagt tgatagttaa tgatgaaaaa aagaccaata aataacagtg
      721 ctgccaataa aaataaaatt atcagcctta tctttatctt ttcgtataaa ctcagcatgt
      781 taatacattt aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct
      841 gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac aacacttgct
      901 tagggcaata atttcctgtg aggtgattac cctttcaagc aatattcaaa cgtaactatc
      961 ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg aacgccagta
     1021 cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata accatatccg
     1081 ctcgtgcctc attgagcatt gctccccact ctggtgtcgg cggctgcgca ccgaggccga
     1141 gaaatgacag gctggcggca gagataatcg aggtgccaat gcgcatggtg aaaaacacca
     1201 cgatagacga gacggtccca ggcaagatat gacgcaataa aatggtcata tcgctggcac
     1261 caatactgcg tgctgactca ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca
     1321 ccagtcgggc aaacgcgggg atggaaaaaa tggcgactgc gataatcacg ttagcaatgc
     1381 cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag gcaaacagca
     1441 catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca gccaacaagc
     1501 ccagcaacgt cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc gccagcgaga
     1561 tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc
     1621 agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt tcggcgtcat
     1681 agggagcgat ccagcgtgca aaaatggcca ccacaatcaa taaaataacg aataatgcgg
     1741 cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgca
     1801 cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata
     1861 gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca catccaccac
     1921 taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta
     1981 atcgcgcatt tctacggagt caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt
     2041 ctccaccacg atagaaccac cgagcaaaaa gccaaactgt aagcccatca tggtcactac
     2101 cgggatcatc gcgttacgta gcccgtgttt gaggacaacc caggtttcgc tcaccccttt
     2161 cgccctcgcg gtacgcatat aatcttcgct taaaacatcg acaaacgacg cgcgggtaaa
     2221 gcgcgccatc acggctgcca ctgccgcgcc gagcgtcagg gaggataaaa tgtagtgctg
     2281 ccaactgtct gctcccacgg taggcagcca gcccagttca acggagaata cctgaattaa
     2341 aagcatcccc agcgcaaatg ccggaaacga gatccccgac accgcaatgg tcatactcaa
     2401 tcgatccggc caacgattac gccagacggc ggcgataatt cccgccgcca taccaaatat
     2461 caccgcccag accatactgg ttatggtcag ccacagcgtt ggcataaagc ggctggcaat
     2521 ctcatcggca accggacgac gcgacaccat cgacaggcca aaatccccct gcacagcatt
     2581 gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct gctgacgcac
     2641 cagttctata acctgcgcat cagcttcggg cccggcaatc aatcgcgccg gatcgccggg
     2701 cagcatatgc acaaataaaa acaccagcac cgagacgata aacagcgtcg gaatcaaccc
     2761 cagtaagcgt ttgataacgt aattaagcat tccactccct gcgttgctta ttgcaaatcc
     2821 gcgtcttcaa agctgaagcc ggtgtctggc atgatccaaa aaccggtcag gtttttactg
     2881 tgtgccgaca ccagtttttc taccaccagc gggatccacg gcgactcttg ccagatgata
     2941 tcctgcgccg ccttatacaa gcgggtcttt tccgccggat cattagtttt cagtgcctga
     3001 gccaggaagt cgtccacctg tttattgctg taaaatgcgg tattaaacag cgtcggtggc
     3061 cagttttgcg aggcgaacag tggcgatagc gcccagtccg cttcgccggt tgaagccgac
     3121 cagccagtgt agaacatccg cacgccgctc tctttttgcc ctttaccttc aacttctgcc
     3181 gcccgctgtc cggcatccat cgcggtcacc tgggctttaa tcccgacctg cgctaactgc
     3241 tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg
     3301 gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg
     3361 taaggccacg gtttataact ttgcgcgtag gcgatacttg gcggtaccac accagtagct
     3421 ggcgttgcat agcccgcaaa ggcaactttc accagcgccg gacggttaat ggcgtaattc
     3481 agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat actgatataa
     3541 cgctgcataa ttgacggact ggccatcaac tcgatatttt tgtttttctc cagcagtgcg
     3601 gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag cattgccgcg
     3661 cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg caatcctggc
     3721 tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca ggtatccagt
     3781 tcatacggtc cggttcccac cggatgaaaa ccaatctcct tgccatattt ttccagcgct
     3841 gccggtgaaa tcatcgcggt cgccggatgg acaagaatat taataaacgc tgagaacggc
     3901 tgtttgaggg taatctttac cgttgtcgga tcgatcgcct ccgttttggc aatattctta
     3961 tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag attcgctttc
     4021 accgccgcgg cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg caatttcacg
     4081 gtgtaagtaa tgccgtcatc ggaaacggta taactctccg ccagcacgtt tttcagtttc
     4141 atctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc ctgagataac
     4201 gtgtcattcg cgtcatacgg atcgagcgtg gtgaaattcg atcctaccgc caccaccaca
     4261 tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag cgccactaac
     4321 ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc tacgcatgaa
     4381 tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt gcaacgagac
     4441 ggctgccacc tcttcgccac gcagatgaat attgctggga agatcgtccg acagcagtac
     4501 acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta atttacgcgt
     4561 ataaggatgc tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa caatttgccc
     4621 gagatacatc accgccacac gatgactaat ccgctctacc acggccatat cgtgggagat
     4681 aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga taatctgccc
     4741 gcgaatagaa acatccagcg ccgaaacggc ttcgtcggca atgatcactt ttggattcaa
     4801 tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact catgcgggta
     4861 acgccaggca tgttcaggta acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc
     4921 cgcgtcttta cctggcaata aaccgtgtac acacagcggt tcgataatcg aatcaccgat
     4981 ggtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg
     5041 caatgcctga agtttgccag gtgacaaggt atcgattcgc tgaccgttaa agataatttc
     5101 gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc
     5161 agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga ctttctcaac
     5221 ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac
     5281 aagattacgc actcgtaaaa caggttcgcc atccaccacc gttttctgct cgatgggggg
     5341 ttcctgtttc gctggatgtt caagcgatat caatgggaaa cgtcggggat aatctaaccc
     5401 tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtgtaag gatgttgcgg
     5461 tgcatgaaaa atctgttcga cggtacccgt ttccaccgcc tcgccctgat acatcaccag
     5521 tacccgatcg gctatctctg ccaccacgcc catatcgtgc gtgataaaga taacgcccat
     5581 cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc
     5641 cagcgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca gcgccatcgc
     5701 aatcatcact cgctggcgca tcccgccaga gagttgatgc ggataacgtg aaagaatggt
     5761 ttgtgcctca ggaatgcgta cctgatccag catccgcttc gcctcgacca tcgcttcttc
     5821 acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca cagtaaatac
     5881 cgggttcagc gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac cgcgaacatg
     5941 gcgcatttgt gcagcgctct gctcgctaag ttcaatcact tcgcgactgc gccgctgcaa
     6001 cagcatttta tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca tcaatgccaa
     6061 cgcagtcact gacttaccgg agccggattc gccaacaatt gccagcgtct caccgcgttg
     6121 cagactaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca taaaggcaat
     6181 attcagattt tcaaccgcca gcacattacc ggcatcaagt tcatcactgt gtggcaacgg
     6241 tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc
     6301 gatacattcc ttcggtgtta aacggtagcg cgacattccc ttcatggccg atagcgatta
     6361 agccaccgct accgccaagc gcagggagtt tttccattac tacccgctcg caggcttccg
     6421 cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc agcgcgcgga
     6481 tgaagacttc gcccgtgccg gtacaagaaa ccgccacact ggcgttattg gcgtagcatc
     6541 cggcacccac taagggacta tcgccaactc gtccgggtaa tttattggtc attccgcctg
     6601 tggacgtggc tgccgccaaa ttgccgtcta aatccaacgc cacggccccc acggtgccca
     6661 ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc ccttccttgc
     6721 gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatcgccggc gagacgcgct
     6781 ccatgccacg agcaaacgca aaattttctg ccccttcgcc aatcatcatc acatgcgggc
     6841 tttgctccat caccagccgg gcggcaagaa ccggattacg cagatgacta acgcccgcca
     6901 ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca tgggtttcat
     6961 cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc agcagacgca
     7021 ccgcttccgt caccacatcc agcgcacttt cgcccgcttc cagcattttc tgcccggttt
     7081 caacaatggc agacaacgcc tcaatgtagc gtaattcctg ttgcagactc atctgcgcgc
     7141 ggctaattgc acctgcgcca ccatgaattg caatgactgc tttgcccata gtattcgtcc
     7201 attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat tactcattca
     7261 atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg ctacgcccga
     7321 ggtggcatac agtctgtggc gtttttctga cataataggc aaattcgatt ttgcctccgc
     7381 aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag
     7441 atgctttctc gcgtcacccc actgaccgcc caggaaacgc tgccactggt acagtgtttt
     7501 ggtcgtattc tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt tgataactcc
     7561 gcaatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt
     7621 gccggtaaat cctttgccgg tcagccgtat catggtgaat ggcctgcggg cacctgcatt
     7681 cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag
     7741 actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt
     7801 cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt tcccggcggg aactcgcctg
     7861 actaccgcag agctgccagt aattgcttca ctggggattg ccgaagttcc ggtgattcgt
     7921 aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg tcagccgctg
     7981 ggtgacggcc aaatctacga caccaaccgt ctcgccgtac acctgatgtt agaacagctg
     8041 ggttgcgagg taatcaactt agggattatc cgcgacgatc cccatgccct gcgcgccgca
     8101 tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt
     8161 gaagcggatt acaccaaaac gattcttgaa gagctggggg agatcgcctt ctggaagctg
     8221 gcgattaaac caggtaaacc gttcgcgttc ggtaaactca gcaatagctg gttctgcggc
     8281 ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca gcctttgctg
     8341 gcaaaactaa gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg
     8401 tcccgcctga aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct gcaacgcaac
     8461 gccgacggtg aacttgaagt gacgaccacc ggacatcagg gttcacatat atttagctcc
     8521 tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgggc
     8581 gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg gcggaactca
     8641 gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt gattttgacg
     8701 gtcaggaggc gctaaaagat tctcgtgtgc tgatcgtcgg cctcggtgga ctcggctgcg
     8761 cagcttcgca gtatctggca agcgccggtg tcggcaacct gacgctgctc gacttcgaca
     8821 cggtttcgct ctcgaatctg caacgccaga cactgcacaa tgatgccacg gtcgggcaac
     8881 cgaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc gcgattacgc
     8941 cagtcaatgc actgctagat gacgcagaac ttgcagcatt gattgctgaa tacgatctgg
     9001 tgctcgactg tacggataac gttgcggtac gtaatcaact gaacgcaggc tgttttgccg
     9061 cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca
     9121 cttatcagga cggcgaaccg tgctatcgct gccttagccg tttgtttggc gagaacgcgt
     9181 taacctgcgt agaagcaggc gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg
     9241 caatggaagc gatcaaactg ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca
     9301 tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgtaat ccggggtgtg
     9361 aggtgtgcgg gcagtaattg ccacctccag gccagataag acgcggcact ccgccacgtc
     9421 atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc
     9481 agccagtcct gttcaaactt cgccacagcg gcatcaaccg ccggatagct aatcatctgt
     9541 tgtgccacat ccagtggcag cgtaattgat tcacatcctg ccagtaagca gtccagcgcc
     9601 tgacgcgggt ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat
     9661 aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta
     9721 acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcagcccttg tgctgcgcca
     9781 tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat agctgccagc
     9841 ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg caatttacgt
     9901 gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga
     9961 ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg
    10021 ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcact
    10081 gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg
    10141 tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatccaactt
    10201 tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt
    10261 cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt
    10321 tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca ggatctgctg
    10381 tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggcagc gccggaagtg
    10441 attgagcgcg cattgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg
    10501 acggcgctaa ccgactgctg cccgacacag gcgttaaccg tgtgtgggga agtgaaaagc
    10561 gttgaggaga tcatggcgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc
    10621 ggcttaacgc tttcgggtgg tgaacccttt atgcagccgg aaatagcgac ggcgctactg
    10681 caagcctgcc acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg
    10741 aaatatatca ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacatt
    10801 gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccaaggtgct ggataacctg
    10861 aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt
    10921 aatgccgacg aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt
    10981 ggcgaaattc attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat
    11041 ctgccctatg acgccccgga aaaaccgctt gatgcgccag acctgctcga ctttgcccag
    11101 cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac
    11161 tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg
    11221 tgaaaccgcc ggtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc
    11281 tcgataagcc gatcccggta cgtcgcgcgc tggctctggc gcatcacctg gcgaatcgca
    11341 ccatctggat caagcacgat gagttgatca tcggcaacca ggcaagcgag gttcgcgctg
    11401 cgccgatctt cccggaatat accgtctcgt ggatcgaaaa agagattgat gatctggcag
    11461 atcgtcccgg tgctggtttt gcggtgagcg aagagaacaa acgcgttctg catgaagtgt
    11521 gcccgtggtg gcgcggtcag acggtacagg atcgctgcta cggcatgttt accgatgagc
    11581 aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc tccggcgatg
    11641 cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt ctgcgcgaga
    11701 aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg cacggcgaac
    11761 agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt gaacgttttg
    11821 cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc
    11881 tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg
    11941 cgctgcaact gtgttacttc atccagttga tattgcagat cgaatctaac ggtcactcgg
    12001 tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgagac gttgaactca
    12061 accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg ctgaaactgc
    12121 tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga agtcctctgt
    12181 atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg gacgcggtga
    12241 atccactctc ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc
    12301 tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc gtacaggtga
    12361 tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc ccggaattta
    12421 ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt tgtatcgaaa
    12481 ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc
    12541 gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac aagcggcaac gtgttcctgc
    12601 cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta atggacgcgt
    12661 gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca
    12721 ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg
    12781 agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg
    12841 ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta tttgaacagg
    12901 gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc
    12961 atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata
    13021 ctgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc
    13081 ataatccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcatcta
    13141 tctccgctaa cgtaccgttt ggtgcgcaaa ctatggcaac gccggacggg cgtaaagccc
    13201 acaccccgct ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg
    13261 cggtcattgg ctcggtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca
    13321 accagaaact gaatccggca acgctggaga acgaatctga caagcagaag ctgatgatcc
    13381 tgctgcgcac cttcttcgag gtgcataaag gctggcatat tcagtacaac atcgtttccc
    13441 gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg
    13501 tcgcgggtta ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg
    13561 ctcgtactga acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc
    13621 attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac
    13681 attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa
    13741 gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca
    13801 cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt tgtcgccagc
    13861 ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt
    13921 gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg
    13981 acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt
    14041 gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca
    14101 ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga gattgacgac
    14161 gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt gatcgacaaa
    14221 ctgcacatag cgttcgatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac
    14281 ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat
    14341 ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa
    14401 atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt
    14461 tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc ggtgctggat
    14521 aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg gttttccctt
    14581 ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt
    14641 atattgtcac atgcaaatca taatggtttc gaatgaaagt taatgagagg tggttatggc
    14701 ttctactttt accagcgaca cattgccagc cgatcacaaa gcagctatcc gtcagatgaa
    14761 gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc taagcgatga
    14821 cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc
    14881 ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat
    14941 taaacgtcgc ggttgtgcgg tgataaaagg ccatttctcc cgcgaacaag cgctaggctg
    15001 ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc
    15061 cggcgataat ttcttcggga cgctcagcac ttcacgtccc gagatttacc ccatctactg
    15121 gtcgcagacg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct
    15181 caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt
    15241 catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag gtcttggagc
    15301 gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc gcgttttcgc
    15361 caacgtcttt aatggcaatc tggcgcaata tgatccctgg catgcggcac atcgtacgga
    15421 agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg
    15481 ctggacagcg ctctctgata tgctgcctgg tcaggggttg ctgcacgttg tgcccattcc
    15541 tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact
    15601 gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat
    15661 tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga
    15721 cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat
    15781 tcctgcggca ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact
    15841 ggaaaaaggc gcatcgccgg gcgacttccc gcacgaggac tatgaaacaa actgggaagg
    15901 acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg
    15961 agagttaatg gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt
    16021 gctgacaagt gcgctttctt aatgcctgat gcggcgttaa cgccttatct ggcctacaaa
    16081 gtcctgcaga ttcaatatat tacaggtact gcgtaggcct gataagcgta gcgcatcagg
    16141 ctattttgca tttgtcatca gccgccgtca ccccgacagt atcggggtga cggataatca
    16201 cttactcgat ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc
    16261 gttccggggt gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg
    16321 aaacaaagat cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt
    16381 ccatatccag gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta
    16441 acttaccaaa cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga
    16501 tgtcgtcctg gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc
    16561 cttcctgctt ccactgactc atccattcga acacggtcag atcattttca aactcatatt
    16621 cgtggtcctg ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt
    16681 ccggttgcag atcgcccacc agcgttttca tcagcgttga tttaccgacg ccgttggtac
    16741 ccagtaccgc aagtttttca ccgacctcca gcagcaggtt gagattttta aacagtggac
    16801 cgttatcaaa ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat
    16861 cctgttcaaa acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga
    16921 ttttatcaat ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa
    16981 agcggctaac gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca
    17041 gcagacgttc acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa
    17101 cgcgcagctc gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac
    17161 ggtcgtgcga gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc
    17221 gaatggtgtc gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggatcag
    17281 caaacagcgc ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg
    17341 ggccgtagtg ctgttccact ggaattccca cgccaagcaa caattcaccg gcgcgagctt
    17401 ccgcagagta accgtccatt tcgccgtatt taacttccag atcggccact ttatagccgt
    17461 cttcctcact catttccggc aaagcataga tgcggtcacg ctcctgcttc acttcccaca
    17521 actctttatg ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat
    17581 cctgacgcag cttaccaatg cgctcgttgg gatcgaggga aacgttaccc aaagtcggct
    17641 ctaagtcgcc gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca
    17701 ggccgtaacg gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc
    17761 cgaactgcat ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac
    17821 aaaccgcgca ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac
    17881 tcaagccaaa ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg
    17941 aacttataat gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa
    18001 atctcgctta acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc
    18061 ggcttttgca gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg
    18121 gttggtcaga atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt
    18181 gccgcggagt accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc
    18241 ttcctgccga aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc
    18301 gttcatgaag gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg
    18361 accaacaccg ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc
    18421 aactggacca ccaaagttga gcgtaagaaa gcaggcccga cctggacgcc gaccgcaaaa
    18481 atgcacgcag agtaccgcgc tgcgggcgaa ccgcttccgg ctgtcgttcc ggcaggtccg
    18541 gataacccga tgggtctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc
    18601 aacgccaact tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa
    18661 gacatcaaat tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag
    18721 ccggtaaaag cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg
    18781 tctaccaccg aagcccagtt tgaaggtcag gaaattgtgc caattaccct gacgaagagc
    18841 gtgcagacag tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgattaaa
    18901 aaccgctccg ggatgccggt tcgtctgaat taatcttcaa aacttaaagc aaaaggcgga
    18961 ctcataatcc gcctttttta tttgccagac cctagttggc cgggagtata actaacaaaa
    19021 catatcccac caacgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc
    19081 agatgcggcg atcgttcgcc atacgtagcg caatcaaact tgccagcgat ccgggtaata
    19141 acccaaagcc acctacgttt accgcccatg ccagtaataa agacggcggc acatagttca
    19201 gcaacaatat ggtactcggc acgttactga tcacctgcga taaaccgatt gccgttaacc
    19261 ataacccggg ttcagataga tgactcacgt tacccaacac gccttgcaac gctggaagct
    19321 gggtcagtaa atggacgtcg ataaacatcg ccataaacac cagcagcagc gtccagtcca
    19381 cactgagcac cacgcgacgt gccagcagcg caaagcctgc cgccacaatc accagtcccc
    19441 acagctcttg tttgaactcc agcgccgtta gaaagacgat atacagcccc agacaactcc
    19501 acaccagccg cggtttccac tccggtgttt gcacccccgt atggtattgc agcacctttc
    19561 cagggaaaca acaccagcac aggagcagga gcgtcagcat cattgcgcca gccagcggtg
    19621 ccatttgggc aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt
    19681 gcgggttgcc aattggcgtc agtagcgaac cagcattgac tgccagcgcc tcaaaaataa
    19741 tcagacgatt aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa
    19801 taaacagcgc gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc agcaccataa
    19861 acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa
    19921 aataaccgct taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg
    19981 tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc
    20041 cgataccaac aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag
    20101 gcaggctcat ttggcaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc
    20161 actaacatgg tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa
    20221 caccaacaag aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc
    20281 tccttctgcc gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc
    20341 cagccgctta agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc
    20401 cacctgacga gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc
    20461 aataagctcg cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac
    20521 gttcactaac tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct
    20581 tcctcattat ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag
    20641 ttgcgaaggt acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata
    20701 tctgtatgta atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta
    20761 tgaatgagtt caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa
    20821 tactgctctc tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttccg
    20881 ataaatagcg gcgtcgcggt acgccgcttc actcctgctt tcatgcaggc ataacgcgtt
    20941 ttggcctgaa aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc
    21001 gtcagtagtt gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg
    21061 gctcgcggat gcggacccct ttccactctt cacgcactct tgcaggtatt gacccttgac
    21121 gccagggtaa gcacatggcg tttgttacga tagtggcata tgaatttaac cctcaaagaa
    21181 tcgcttgtta cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg
    21241 ctattaatta acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg
    21301 gctattttgc ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc
    21361 agttcgctgg tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc
    21421 aatacattgc tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt
    21481 tttccgtggt acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc
    21541 aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta
    21601 ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt
    21661 aaactgaaag atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat
    21721 aacgaggtga ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg
    21781 acggtcactg ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg tgaaagcgcg
    21841 cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc
    21901 gttaacggtt tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt
    21961 ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc
    22021 ctggcaaatc gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa
    22081 tacgatactg ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa
    22141 ggtaattttg aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa
    22201 gtgctggcgc aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat
    22261 ccgcaggcct gcgacaggac acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg
    22321 tcgtgctatc tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag
    22381 ttacgcaaca gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt
    22441 aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc
    22501 caggtgcctt ttatggtcat ttccagcgac gataaagcgc atcgggtgat taaagcccgc
    22561 cgctcagcca atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa
    22621 ataaatatca aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac
    22681 ttccagttac agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct
    22741 taaaaacaaa aatccgcccc gagaggcgga ttttttatat caccaaagtg attagaagcg
    22801 gtaaccaaca ccggcaatcc aggtgcctac gtcaacgcta cgaatacggc tctgctcgta
    22861 agagaagtcc agagcaacgt tttccatcgg gttgaactgc agaccagcac cgtaggagaa
    22921 accgtagtcg ctggtgtcgt gtttgtaggt cgggtattca gtggtctgga atttaccata
    22981 acccacaccc actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc
    23041 agtgatgccg tagtactggt ttttgttgta gtcaccagag cttgcagtac gacttttctc
    23101 ggtgtaagtg aaagaaccga tcacacccag cgggctgttg tcttcttcat agcggtattt
    23161 caggttgaaa ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc
    23221 agttacggta gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag
    23281 tgctgaaaga catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa
    23341 gttttaaata tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc
    23401 atttcatgta acagaacgtt tccatacacc gctatccatc taaatttaaa tcactttttc
    23461 agagaactgc gtaagtatta cgcatgtttt ccctgtcatt catccagatt attcctaatc
    23521 accagactaa tgattccatc aatcctggcg cattttagtc aaaacggggg aaaatttttt
    23581 caacaaatgc tcaaccagca ttgggtatat ccagtacact ccacgcttta cttaagtcta
    23641 gatatttgtg ggagaaagga tgcctggttc attacgtaaa atgccggtct ggttaccaat
    23701 agtcatattg ctcgttgcca tggcgtctat tcagggtgga gcctcgttag ctaagtcact
    23761 ttttcctctg gtgggcgcac cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat
    23821 cctcatcgcg ttctttaagc catggcgact gcgctttgcc aaagagcaac ggttaccgct
    23881 gttgttttac ggcgtttcgc tgggtgggat gaattatctt ttttatcttt ctattcagac
    23941 agtaccgctg ggtattgcgg tggcgctgga gttcaccgga ccactggcgg tggcgctgtt
    24001 ctcttctcgt cgcccggtag atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt
    24061 cctgctaccg ctggggcaag acgtttccca tgtcgattta accggctgtg cgctggcact
    24121 gggggccggg gcttgttggg ctatttacat tttaagtggg caacgcgcag gagcggaaca
    24181 tggccctgcg acggtggcaa ttggttcgtt gattgcagcg ttaattttcg tgccaattgg
    24241 agcgcttcag gctggtgaag cactctggca ctggtcggtt attccattgg gtctggctgt
    24301 cgctattctc tcgaccgctc tgccttattc gctggaaatg attgccctca cccgtttgcc
    24361 aacacggaca tttggtacgc tgatgagcat ggaaccggcg ctggctgccg tttccgggat
    24421 gattttcctc ggagaaacac tgacacccat acagctgctg gcgctcggcg ctatcatcgc
    24481 cgcttcaatg gggtctacgc tgacagtacg caaagagagc aaaataaaag aattagacat
    24541 taattaaatt tacatttctg catggttatg cataaccatg cagaatttct cgctactttt
    24601 cctctacacc gtctttatat atcgaattat gcaaaagcat atttattccg aaaattcctg
    24661 gcgagcagat aaataagaat tgttcttatc aatatatcta actcattgaa tctttattag
    24721 ttttgttttt cacgcttgtt accactatta gtgtgatagg aacagccaga atagcggaac
    24781 acatagccgg tgctatactt agtctcgtta attactggga cataacatca agaggatatg
    24841 aaattatgag taccgctaaa ttagttaaat caaaagcgac caatctgctt tatacccgca
    24901 acgatgtctc cgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc
    24961 agtttattga tctttctttg attaccaaac aagcgcactg gaacatgcgc ggcgctaact
    25021 tcattgccgt acatgaaatg ctggatggct tccgcaccgc actgatcgat catctggata
    25081 ccatggcaga acgtgcagtg cagctgggcg gtgtagctct ggggaccact caagttatca
    25141 acagcaaaac cccgctgaaa agttacccgc tggacatcca caacgttcag gatcacctga
    25201 aagaactggc tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag
    25261 cgaaagatga cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg gataaattcc
    25321 tgtggtttat cgagtctaac atcgaataaa tccatcgctg atggtgcaga actttagtac
    25381 ccgataaaag cggcttcctg acaggaggcc gttttgtttt gcagcccacc tcaacgcact
    25441 tatttagtgc atccatctgc tatctccagc tgattaagta aattttttgt atccacatca
    25501 tcacacaatc gttacataaa gattgttttt tcatcaggtt ttacgctaaa taatcactgt
    25561 gttgagtgca caattttagc gcaccagatt ggtgccccag aatggtgcat cttcagggta
    25621 ttgccctata aatcgtgcat cacgtttttg ccgcatctct aaaaatcaat gagttgcaaa
    25681 actggcacga ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa
    25741 aaaggaaatg ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac cctggttttt
    25801 gcggtttctt ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt
    25861 ccgtttgaat ttaaacaggg cgataaatat gtgggctttg acgttgatct gtgggctgcc
    25921 atcgctaaag agctgaagct ggattacgaa ctgaagccga tggatttcag tgggatcatt
    25981 ccggcactgc aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat caccgacgag
    26041 cgtaaaaaag cgatcgattt ctctgacggc tactacaaaa gcggcctgtt agtgatggtg
    26101 aaagctaaca ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg
    26161 aagagcggta ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt
    26221 cagttcccga acatcgataa cgcctatatg gaactgggca ccaaccgcgc agacgccgtt
    26281 ctgcacgata cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa
    26341 gcggtaggtg actctctgga agcgcagcaa tacggtattg cgttcccgaa aggtagcgac
    26401 gagctgcgtg acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac
    26461 gaaatctaca aaaaatggtt cggtactgaa ccgaaataat aacgctacac ctgtaaagcg
    26521 cactggcagt tccctctccc ctctggggag aggattaggg tgaggggcgc aaacccgctc
    26581 cggggccatt aattaccctg aatttgatta tttacaccac ggtaacagga acaacatatg
    26641 cagtttgact ggagtgccat ctggcctgcc attccgcttc tgattgaagg tgccaaaatg
    26701 accctgtgga tttcggtcct cggtctggct ggcggtctgg taatcggatt gctggcaggt
    26761 tttgcacgca ccttcggtgg ttggatagcc aaccacgtcg cgctggtctt tattgaagtg
    26821 atccgcggca cacctatcgt cgtccaggtg atgtttatct atttcgccct gccgatggcg
    26881 tttaacgact tacgcatcga cccatttact gcggcggtgg tcaccatcat gatcaactcc
    26941 ggcgcgtaca ttgcggaaat cacgcgtggt gcggtgctgt ctattcacaa aggttttcgt
    27001 gaagcaggac tggcgctcgg tctttcacgt tgggaaacca ttcgctacgt cattttaccg
    27061 ctggcactgc gtcgtatgct gccgccgctg ggtaaccagt ggatcatcag cattaaagac
    27121 acctcgctgt ttattgttat cggcgtggcg gaactgaccc gtcaggggca agaaattatt
    27181 gccggtaact tccgcgccct tgagatctgg agcgccgtgg cggtgttcta tctgattatt
    27241 accctggtgc tgagctttat tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg
    27301 aatttaaaaa cgtctccaag cactttggcc caacccaggt gctgcacaat atcgatttga
    27361 acattgccca gggcgaagtc gtggtgatta tcgggccgtc cggttccggt aaatcgaccc
    27421 tgctgcgctg catcaacaaa ctggaagaaa tcacctccgg cgatctgatt gtcgatggcc
    27481 tgaaggttaa cgatccgaaa gttgacgagc gcctgattcg ccaggaagca ggtatggtgt
    27541 tccagcagtt ttacctcttc ccgcatctga cggcgctgga aaacgtcatg tttggtccgc
    27601 tacgcgtgcg tggcgcgaac aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga
    27661 aagtcggtct ggcagaacgt gcacatcact acccttccga actttctggt ggtcaacagc
    27721 agcgtgtggc gattgcccgc gcgctggcgg tgaagccgaa aatgatgctg tttgatgaac
    27781 cgacttccgc tcttgacccg gaactgcgcc atgaagtgct gaaggttatg caggatctgg
    27841 ctgaagaagg gatgacgatg gtgatcgtga cccacgaaat cggttttgcc gagaaagtag
    27901 cttcgcggct gatctttatc gacaaaggcc ggattgcgga agatggcaat ccgcaggtgt
    27961 tgatcaagaa cccgccgagc cagcgcttgc aggaattttt gcagcacgtc tcttaataag
    28021 acacattgcc tgatgcgcta cgcttatcag gcctacagga tatctggcaa cttattaaaa
    28081 ttgcatgaac ttgtaggacg gataaggcgt tcacgccgca tccggcaaaa aagcccgcac
    28141 gttgtcagca atctgcttaa tatcccttcc tccctttcac ccgaaaggga ggcacaccag
    28201 attcctctca tttaaaatcg cccc
//
LOCUS       DAJVOY010000035        27842 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVOY010000035 DAJVOY010000000
VERSION     DAJVOY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.035"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..517
                     /locus_tag="OQL90_001346"
     CDS             <1..517
                     /locus_tag="OQL90_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1368177.1"
                     /translation="IMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEIINK
                     KHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGND
                     GRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLM
                     PLIRRRTMLQL"
     gene            883..1950
                     /locus_tag="OQL90_001347"
     CDS             883..1950
                     /locus_tag="OQL90_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1368178.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1947..4682
                     /gene="rbbA"
                     /locus_tag="OQL90_001348"
     CDS             1947..4682
                     /gene="rbbA"
                     /locus_tag="OQL90_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1368179.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4682..5806
                     /locus_tag="OQL90_001349"
     CDS             4682..5806
                     /locus_tag="OQL90_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1368180.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5879..6153
                     /locus_tag="OQL90_001350"
                     /pseudo
     CDS             5879..6153
                     /locus_tag="OQL90_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6150..6509
                     /locus_tag="OQL90_001351"
     CDS             6150..6509
                     /locus_tag="OQL90_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1368181.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6629..7030)
                     /gene="nikR"
                     /locus_tag="OQL90_001352"
     CDS             complement(6629..7030)
                     /gene="nikR"
                     /locus_tag="OQL90_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1368182.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7036..7842)
                     /gene="nikE"
                     /locus_tag="OQL90_001353"
     CDS             complement(7036..7842)
                     /gene="nikE"
                     /locus_tag="OQL90_001353"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1368183.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7839..8603)
                     /gene="nikD"
                     /locus_tag="OQL90_001354"
     CDS             complement(7839..8603)
                     /gene="nikD"
                     /locus_tag="OQL90_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1368184.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8603..9436)
                     /gene="nikC"
                     /locus_tag="OQL90_001355"
     CDS             complement(8603..9436)
                     /gene="nikC"
                     /locus_tag="OQL90_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1368185.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9433..10377)
                     /gene="nikB"
                     /locus_tag="OQL90_001356"
     CDS             complement(9433..10377)
                     /gene="nikB"
                     /locus_tag="OQL90_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1368186.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQFFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10377..11951)
                     /gene="nikA"
                     /locus_tag="OQL90_001357"
     CDS             complement(10377..11951)
                     /gene="nikA"
                     /locus_tag="OQL90_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1368187.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12062..12649)
                     /gene="acpT"
                     /locus_tag="OQL90_001358"
     CDS             complement(12062..12649)
                     /gene="acpT"
                     /locus_tag="OQL90_001358"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1368188.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12704..13753)
                     /locus_tag="OQL90_001359"
     CDS             complement(12704..13753)
                     /locus_tag="OQL90_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1368189.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVTITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMIGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYACILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13885..15102
                     /locus_tag="OQL90_001360"
     CDS             13885..15102
                     /locus_tag="OQL90_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1368190.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15106..15663)
                     /gene="dcrB"
                     /locus_tag="OQL90_001361"
     CDS             complement(15106..15663)
                     /gene="dcrB"
                     /locus_tag="OQL90_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1368191.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15736..16401)
                     /locus_tag="OQL90_001362"
     CDS             complement(15736..16401)
                     /locus_tag="OQL90_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1368192.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16622..16867
                     /gene="tusA"
                     /locus_tag="OQL90_001363"
     CDS             16622..16867
                     /gene="tusA"
                     /locus_tag="OQL90_001363"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1368193.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16969..19167)
                     /gene="zntA"
                     /locus_tag="OQL90_001364"
     CDS             complement(16969..19167)
                     /gene="zntA"
                     /locus_tag="OQL90_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1368194.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19241..19867)
                     /locus_tag="OQL90_001365"
     CDS             complement(19241..19867)
                     /locus_tag="OQL90_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1368195.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20008..20367
                     /locus_tag="OQL90_001366"
     CDS             20008..20367
                     /locus_tag="OQL90_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1368196.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20370..20639)
                     /locus_tag="OQL90_001367"
     CDS             complement(20370..20639)
                     /locus_tag="OQL90_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1368197.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20629..21225)
                     /gene="rsmD"
                     /locus_tag="OQL90_001368"
     CDS             complement(20629..21225)
                     /gene="rsmD"
                     /locus_tag="OQL90_001368"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1368198.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21375..22868
                     /gene="ftsY"
                     /locus_tag="OQL90_001369"
     CDS             21375..22868
                     /gene="ftsY"
                     /locus_tag="OQL90_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1368199.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22871..23539
                     /gene="ftsE"
                     /locus_tag="OQL90_001370"
     CDS             22871..23539
                     /gene="ftsE"
                     /locus_tag="OQL90_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1368200.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23532..24590
                     /gene="ftsX"
                     /locus_tag="OQL90_001371"
     CDS             23532..24590
                     /gene="ftsX"
                     /locus_tag="OQL90_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1368201.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24835..25689
                     /gene="rpoH"
                     /locus_tag="OQL90_001372"
     CDS             24835..25689
                     /gene="rpoH"
                     /locus_tag="OQL90_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1368202.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25960..27063
                     /gene="livJ"
                     /locus_tag="OQL90_001373"
     CDS             25960..27063
                     /gene="livJ"
                     /locus_tag="OQL90_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1368203.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27262..27645)
                     /gene="panM"
                     /locus_tag="OQL90_001374"
     CDS             complement(27262..27645)
                     /gene="panM"
                     /locus_tag="OQL90_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1368204.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6587 a         7225 c         7741 g         6289 t
ORIGIN      
        1 aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt acattatagg
       61 atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg aattagcgga
      121 aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa atagccgggc
      181 tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca gacctaaact
      241 tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg cacataacgg
      301 taatgatgga agagaatgta taggtgaaac ggggacagtt ttaggtctta acgcccgtga
      361 cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc ctggtgcagg
      421 gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa atggacttat
      481 gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca tgaatattaa
      541 tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg ttcctgcaac
      601 tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc agttttatag
      661 atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga tgacaacatt
      721 actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg ctggtcgtat
      781 ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg gcggcagttg
      841 taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa gagtaagcgc
      901 catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc gtggtggctg
      961 ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat tgaagcgacg
     1021 gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt gaaagaaggc
     1081 cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgtgtgtt gcaggaacag
     1141 cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc cgcgcaggct
     1201 ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa tcaacgccag
     1261 gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc ccaacgaggg
     1321 gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc ccgagctgcg
     1381 ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc ggcacgtacc
     1441 aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg gcgcattgcc
     1501 gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca gtatcgggtt
     1561 gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt cgatctcagc
     1621 gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa actgggcggt
     1681 gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac catcagtttt
     1741 gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga acggctgaaa
     1801 ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca tctggaatat
     1861 gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact tccgtggcct
     1921 gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc gtcccgcctg
     1981 tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg aacaatatca
     2041 ctctcgatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc gtcgggaagt
     2101 cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat gtgatggtgc
     2161 tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc atcgcctgga
     2221 tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa aacgtcgatt
     2281 ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt aatgagctgc
     2341 tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc tccggcggga
     2401 tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg ttgatccttg
     2461 atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat ctgatcgaca
     2521 gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat atggaagagg
     2581 ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca accggcagcg
     2641 ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt attaatctgt
     2701 taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat caacctgaaa
     2761 acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc ttcgttgccg
     2821 ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt ggttcgaacg
     2881 gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc agcgaaggtg
     2941 aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc cgtcgtgtgg
     3001 gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa aacctggagt
     3061 tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg gctgaaatga
     3121 gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg ccgctcggca
     3181 ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg ttaatcctcg
     3241 atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag ttgatggtcg
     3301 atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg aacgaagcgg
     3361 aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc ggtacaccgc
     3421 aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc gcctatttgc
     3481 aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac gacaccaccc
     3541 acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc cgcgaagcgc
     3601 tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg gtgatcctga
     3661 tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt gcggtgctcg
     3721 accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt tcccgttact
     3781 ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg cgtgcgggcg
     3841 atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg cgtggtacgc
     3901 ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa acggtaaaag
     3961 gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga caatcgacac
     4021 ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac ccggacgtaa
     4081 aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg atcccgtcaa
     4141 tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc aacctttacg
     4201 tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac atcgcgctgg
     4261 ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc gtaccgcata
     4321 aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt gccaccggaa
     4381 tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc ggaacggcga
     4441 ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta gcttcgctgg
     4501 aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg actatcgccc
     4561 gcgggacgtt ctcgaaagcg ctggatctga ctgatatgtg gcaacttttt atcccgttgc
     4621 tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa caggagggat
     4681 gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca gtctgctcgg
     4741 tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt attcgtcagc
     4801 gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata tggatcaatc
     4861 gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc caccggagat
     4921 gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct tcgcgataaa
     4981 tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata ttcaggtgaa
     5041 cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc agaatattat
     5101 caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac cgttggtatc
     5161 gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg gcggggtgat
     5221 ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg cgctgatccg
     5281 cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc cgtttgagat
     5341 catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg gattatcgct
     5401 ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc cgctgtttat
     5461 gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta tggggacgat
     5521 agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc cgctgcaaat
     5581 gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg acattatgct
     5641 gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc ggggtgccgg
     5701 attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg cgtttttcac
     5761 cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa gggaaacttg
     5821 acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag gacatgccat
     5881 gagaaccaag gtacaggctt tacggaagaa acaaaaaata cattggatca aatatttaaa
     5941 actcctgttc ctcaagggat caagtggtct gatatagagt cactggttaa agcattaggc
     6001 ggagaaatta aggaaggaag aggttcgcgt tgtaagttca tactaaatat gagcgttgcg
     6061 tgtttccatc ggcctcatcc gtcgccagat accgataaag gcgctgtaga aagcgtgcgt
     6121 gactggttac taagtatagg ggtaaaacca tgatcaaact gaagacgcca aactctatgg
     6181 agattgccgg gcaaccagct gttattacct acgtgccaga actgaatgca tttcgtggca
     6241 aattcctggg cttgtcaggt tattgtgatt ttgtctctga cagcattcag gggctgcaaa
     6301 aagaagggga actttccctg ctagagtatc ttgaggattg taaagctgcg ggtattgagc
     6361 cgtatgcgcg tacagaaaag attaagacct ttacgttacg ctatccagaa tcgctgagtg
     6421 aacgccttaa taatgcggct gcgcagcaac aagtctctgt taatacctat attattgaaa
     6481 cactgaatga gcgtttgaat catttataaa ttttagcggc gtacattacg cagaatatct
     6541 gcctttacag gatctatcta tgcaactgaa cttaatttta taaattaagt tcagttaata
     6601 tcttttcttt cctcgtttca gaaataactc aatcttcctt cggcaagcac tgcaaatgcc
     6661 catgccgcac gccgcgctgg gcgataacgt catcggcaaa atgctgcacg tcgcccatat
     6721 cacctttcag cacggcgatt tccagacagt cgtcgtgatt gatatgcaca tgcagcgtgg
     6781 cgacggagag atcgtggtga tggtgctggg tggagacaat gcggctggct aagtcgcgtt
     6841 tttcgtgttc atacacatag gagagcaccg cgaaaccttg cgtgccgtgc tgctgagtgg
     6901 cctcctgggc cagggcgcta cgcagaatat cgcggatagc ttcggaacgg ttgttataac
     6961 cacgacgctg gctcaggctg tccagcgtct ccagtaaatc gtcatcaagc gtgatggtga
     7021 ctcgttgcat ttgcgttaaa ccttttctgt ggcgcgacgg cgcacgggga atgcgggtaa
     7081 taccgcgttt tgtagcacac gtccggcgtc agaggaaaag gttaatttat ctcccaccgc
     7141 ctgggtttcg acgatttgtc cgttgtccat caccatcacc cgctggcaaa agcgttctac
     7201 aagacgtaag tcgtgggtga tgaacaggca ggcggtgcca aactgttgtt gcagcttttt
     7261 cagcaggcga atgacgcccg cctgtaacac gagatcaagg ttagaaacgg tttcatccag
     7321 aatcagcaat ttcggttcga ccgccagcgc gcgtgccagg cagacgcgct ggagctggcc
     7381 gccgcttaac tgcggtgggc gtttgtcgag aacgctgtca tcgagatcga ccgccttcag
     7441 catttcgctg gcgcgcgcca gttgttcgga ttttttcagt gatagcaggt ggcgcatcgg
     7501 ttcacgcagg atctcgcgca cggttttgcg cggatttacg gcactgatgg agtcctgaaa
     7561 caccatctga atatcacggc ggaaggcttt acgttgggcg cgattaagtt tcgccagcga
     7621 ttcgccgcgc cagctaatat tcccttgtga gggagactct aagcctacca gcagccgcgc
     7681 gagggtgctt tttccgcagc cgctgcgtcc cagcagggcg acggtttcgc cgcttttcag
     7741 ggtcagggaa acgttattca gcaccgcctg atgttgatgt tttccgctaa atccaccgtg
     7801 ggcatagtga tgggaaaggc cgctgacgtt aagtaaggtc atgatgccag ctccataccg
     7861 tagagggcga gatgagcgga aaccaggctg cgcgtcaccg tatgtttggg ggcgttaaac
     7921 agcgtttcta catcgccctg ttcgacaatt ttaccttgtg acatcaccgc cacgtcatcc
     7981 gccagacgcg ccacaacgcc catatcatgg gtgaccagca gcattcccgg cgcttgtttt
     8041 tgcataatgc tttccagcag atcgaggatg cgcgcctgtg ctaccacgtc gaggtcggtg
     8101 gtcggttcat cggcgatgat aaacggtgat tcacacagca ccgccatcgc aatcatcatg
     8161 cgctgcaaca tgccgccgct catctcgaac gggtacagct tcagcacgcg cgcggcgttt
     8221 tccagcccca ccgcttctat ggcagcggta agcgtagcgt catcggcggg tttccctaac
     8281 gccaggcagg tttcccgcgc gtgggtgtgc atggtgtgta gcggattaaa ggcgctgcgc
     8341 gggttctgca tgatggtggc aattttgatg ccgcgcaggg cgcagggata aaccgctttg
     8401 ccatcggcta aaatttcccc cgccgtctgg cgaacgccag cgggcaaaat gcccagcgtc
     8461 gcggcgcagg ttaatgattt cccgctgccg ctaccgccga ctaacgccag cacgcgcccg
     8521 cgttgcaggg ttaacgatac accgtgtacc agcggctgcg cggcctgtag cgcgatatta
     8581 cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt caccagatga gggtccagat
     8641 gatcgcgcag tgcgtcaccc accaggttaa aggccatcac gctgataaac agcgccagcc
     8701 ccggccagaa catttgcagc ggctgggtcc agatatactg gcgcgcgtcg ttaatcatca
     8761 cgccccattc ggcggtcggc gcggtcacac cgaggccaag gaaagacatc cccgagacgt
     8821 gcagcatcat atggccgata tccagcgttg ccagcaccag cagcgaaggg atcaccgcgc
     8881 ctgccagatg atcgacaaac actcgcacat ggcccgcacc ggaaagccgt gacgccagca
     8941 caaactcgcg ttggcgtagt gaaatcacca ggctgcgcac catgcgtgca taccacgccc
     9001 agtgcgacag ggcgatggcg ataattacgt tggtcagccc ggtgccgagc acgccaacca
     9061 taaagaacga cagaatcgag gtcgggaagg tcataaacat atcggcgacg cgcatggtgg
     9121 cctgatcaac gcgcccgcca atcaacccgg cgctgccgcc aataaccagc cctaatgtca
     9181 gcaccagcag caggcaggcc attaccgaac cgagcgacac gcgggtcgct gccatcagcc
     9241 gcgagaaaat atcgcgacct aagtgatcgg tgcccagcca gtgctgcgca tccggcgaaa
     9301 gcaggtgcga cggcaaatca atcgcctggg ggtcatacgg cagccaccac tggctggtga
     9361 gcgcaatcag cgccagcagg gcgataatga tcagcgccag gcgtaccgac cagcgggaag
     9421 agaggaaaaa gttcacgcgt gcgctccttc atgacgacga atgcgcgggt ccagcgcggc
     9481 gttgagcaaa tcgacaatca aattacagac cacaaaaacc accaccatca tcagcgtaaa
     9541 gaactgaatc accggatagt cacggttaaa aatcgccgac accgcatagc gcccgacgcc
     9601 aggccaggca aagatgtttt cgataatcat cgtcccgcca atcaattcgc caatgtgcat
     9661 ccccacggcg gtgatcatcg gcagcgaggc attgcgcaga atatggcggc gttcggtctg
     9721 tttgtcattc aggccgcgta gccgcgccca ggtgacgtga cgctgaccgg cgacgtccag
     9781 catactggcg cgcagcagtc gcgcgttaat cgccagcgac ataaaggcga tggaaaccgc
     9841 aggcaaaatg atgtgctgcc agccgccgta gcccatcgcg ggtagccatt gcagatacac
     9901 cgaaaacgcc atcactagca aaaatgccag ccagaagttg ggcatcgaca cgccaagaaa
     9961 cgcgataaag cgcacggcga aatccggcaa acgatcgcgg tggcgcgccg cccagatacc
    10021 gagcggtacg gaagtgagca gaattaatac cagcgccgca cctgcaagtt ccagcgtggc
    10081 gggcaggaag ttcagcatat cgtccagcac cgggcgttgg ctggcgaatg agataccaaa
    10141 gtcaagatgc agcgccttcc acaaccaggt gccgtactgg acgtacagcg gctgatccag
    10201 tccgagcatg gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt tagacagacg
    10261 caaataatcg agcgccgggt cgccggtacc gaggcgcagc atcagaaaaa tgatcaccga
    10321 ggcggcgagc accatcggga tcagcagcag aaagcggcgt aatacgtaac gcaacattaa
    10381 ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg cgatcggcgc gtaggggatg
    10441 ttacccagct ccggttttga taccaccatc attgagatgt aactgatagg cagataaacc
    10501 gcctcgtcat gcagacgggt cagaatgtcg cgatacagcg cctgacgttg cgtttcgtca
    10561 tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg gtttgtcggc taatccttgc
    10621 tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc gtgtggatcg
    10681 tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg ctgacgagca
    10741 tagatactgc tctcttcttc gccaatcagc gagacatctg cgccaatctg gcgcatatca
    10801 gcctgaatga tttccgccat cgatttgctt aacgcatcgg tgccgatgaa cgacagttca
    10861 atgcgcagcg gctgaccatt tttctcgcgg atgtctttgc ccgcaggcag cgtccaaccg
    10921 gctttttcca gcaacgcttt cgctttctgc ggatcgtact ggcgcggttt caggccgagg
    10981 ttggcgtagg gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt gccatacaac
    11041 gcgttatcaa tcagcgattt tttgtttacc gcgtaattaa gagcttcacg tactgccagc
    11101 tcattggtgg gggttttggc ggtattaagc gccagcatca cggtttcgat cggctgtgac
    11161 agttgggtat ggtaagccgg gttctggcta aagcgggcga aggtatcgag cggtaataac
    11221 ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc gcgggtagtc
    11281 gggtccggga tgacgttaaa ggtgatcttt ttaatcgctg gcttttcgcc ccagtagttt
    11341 tcgttacgga cgaagacatc gtactgattc agtttcgatt cctgcaaaat ccacggtccg
    11401 gtgccaatcg gcgctttaat tccgttcatg gtttcatggt ttttaaactg cgagggagcg
    11461 ataaaacgga aaggacgggg cagggccagt tcttgcagga aaggatagta ggcgcttttc
    11521 agggtaattt gcagctctgt tttactgagt gctttaacat caacaatctg gtttgccagc
    11581 tccagccagg cgtgacgttg acggttatcg agcactgcgc ggaagttttc tgccgccgcc
    11641 tcggcatcga acggttcacc gttggataat ttcacgtcat cacgcagggt gaaggtccag
    11701 gttttaccat cttctgaatg agtccagctt tttgccagcc acgggatcac cgaaccgtct
    11761 gcctgatatt tcaccaatgg ttcataaacc atgctctggg cgaacatctg gttaggcgtg
    11821 taaaggtgcg ggtttagtgg cccgacattc accggccagg cggtagtgat ttcatctggt
    11881 gcagcggcat ggacgataaa agacgcacaa gccagcagcg caaatagagt gcggcggagt
    11941 gtggagagca tgataacccc aatggattaa aatagatggc ggaaataagt atgacgattt
    12001 taagtattcg tcatactgat tacctgttct ggatcaatag atgggcagtc gggtgggcgg
    12061 atcagttaac tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag ggggtggggg
    12121 tgcagatcgc aaggcttaaa ttttctagct gacaatggct gactgacagc gaggagtgat
    12181 aggtgctatc tacgctgacg atttgccagg cgctgccgcc acgctgttta acgatggctt
    12241 ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg atcaggatgc acggcgtcca
    12301 tttcagcgtg ttccccgagg ctgaacacgg cgttcgccag ccagcgccag ttggcgcgcg
    12361 ggcgaatcac ttcgatatcg cagccgactt cgccttcatc gctcaacagc agagcgatat
    12421 cgtcaccgct atggcttaag ttgaaccata gcggcgtttc cggcgcgaat gcaggtttgc
    12481 cttgttcgcc atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc aatgcacgcc
    12541 ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa cccggtggca
    12601 gtggagctgc gcttaaggtc gaaactttcc ccagaactat ccgatacata tcagggccaa
    12661 cgtttaatgg aaaatgaaag tgcgtatcgt atcacttgtc gcctcatccc ggtaaccgac
    12721 ttttcggtct gcctggcccc agtaaaatcg ccagtttgct accgcctttg gtggtttcca
    12781 tccagatttt acacacgctg gttaatggca ccgaaagtag catccctacc gggccgagca
    12841 gccatcccca aattaacaat gaaagaaata ccaccatggt ggacatcccc aggcgatggc
    12901 ccatcatccg tggttctaaa atattgccga tgaccatatg gaccactaaa aacaatgcgc
    12961 cgaccagaat acatgcgtaa acaccattaa acagcagcac ctgaatcatt ggcggtacgg
    13021 cggaaattac cgcgccgata ttgggcacgt agttgagcaa aaacgccagt actgcccaca
    13081 tcagcgcaaa ctgtacgccc attagcgcca gccccagcca gacgatgaca cctgtccata
    13141 aactgagtag cgtctttaac gccagataat gcgaaacgcc tttaagtgcg cggtgtaatc
    13201 ctgcgatgtg aatctgtggg ttattcagcg caaaacgcat tttgtaaggg acgtggcgca
    13261 cttcaaacag cataaaaact acggtcatca ccagcaaaag cacgctcgcc attgccccgg
    13321 aaagcccgat cattagcgct gtggtgaagg taaccacttt ttccgagtcc atccgctgca
    13381 gcattcgctc cggcgacata tgcaaattaa gaaaaggcaa catctcctgc aatttaaaaa
    13441 gtttgcgcgt cagctcctta ttaaacttcg gcagcataga gataaattcg ttaaacgatg
    13501 ccgccagtac gccgactagc gcggttagtg cgatcagcat caccaccact acaatcgtaa
    13561 tggtaacggg gcgttgtact ccccgacgaa taaaccaggt aacgagcggg ttgaggacga
    13621 tggcaaaaaa cagcgccagt aacagctgca caatgatatc tgccgctgcg tgaatgcccg
    13681 cgaggatcac taccagcgag gccaacttga gcagaatgtg catgcccgtt ttatcgggtt
    13741 gaggggtttc cattggggct tccttgtgac tttttgtgtt aagtgtagcg ggagtcacgt
    13801 cagcaatatt ctgattctta ctgctgattt tcacgtcagc acgtggtaaa aatgaaacac
    13861 tgttgtaaaa atgtggtgat cctcatgccc gaacccgtag ccgaacccgc gctaaacgga
    13921 ttgcgcctga atttgcgcat tgtctccatt gtcatgttta acttcgccag ctacctcacc
    13981 atcgggttgc cgctcgctgt attaccgggc tatgtccatg atgtgatggg ctttagcgct
    14041 ttctgggcag gattggttat cagcctgcaa tatttcgcca ccttgctgag ccgtcctcat
    14101 gccggacgtt acgccgattt gctgggcccc aaaaagattg tcgtcttcgg tttatgcggc
    14161 tgctttttga gcggtctggg atatctgacg gcaggattaa ccgccagtct gcccgtcatc
    14221 agcctgttat tactttgcct gggacgcgtg atccttggga ttgggcaaag ttttgccgga
    14281 acgggatcga ccctgtgggg cgttggcgtg gttggctcgc tgcatatcgg gcgagtgatt
    14341 tcgtggaacg gcattgtcac ttacggggcg atggcgatgg gtgcgccgtt aggcgtcgtg
    14401 ttttatcact ggggcggctt gcaggcgtta gcgttaatca ttatgggcgt ggcgctggtg
    14461 gccattttgt tggcgatccc gcgtccgacg gtaaaagcca gtaaaggcaa accgctgccg
    14521 tttcgcgcgg tgcttgggcg cgtctggctg tacggtatgg cactggcact ggcttctgcc
    14581 ggatttggcg tcatcgccac ctttatcacg ctgttttatg acgctaaaag ttgggacggt
    14641 gcggctttcg cgctgacgct gtttagctgt gcgtttgtcg gtacgcgttt gttattccct
    14701 aacggcatta accgtatcgg cggcttaaac gtagcgatga tttgctttag cgttgagata
    14761 atcggcctgc tactggttgg cgtggcgact atgccgtgga tggcgaaaat cggcgtctta
    14821 ctggcggggg cagggttttc gctggtgttc ccggcattag gtgtagtggc ggtaaaagcg
    14881 gttccgcagc aaaatcaggg ggcggcgctg gcaacttaca ccgtatttat ggatttatcg
    14941 cttggcgtga ccggaccact ggctgggctg gtgatgagct gggcgggcgt accggtgatt
    15001 tatctggcgg cggcgggact ggtcgcaatc gcgttattac tgacgtggcg attaaaaaaa
    15061 cggcctccgg aacacgtccc tgaggccgcc tcatcatctt aaaacttact gaataaccag
    15121 cgtattaatg atgttttctg cggtggtctg cgctttttgc tgatcgtcag cgggcagcgt
    15181 aatttgcatg gtcagcagtt gattacccac gttacccaga ataacggaag agtacgccgt
    15241 ctggcctttc gcggagataa tactgtctaa ctgctgcatt ttgtgacctt tcagctcaat
    15301 ggctttattg gttaccactt gcagctgcgg atcgcggcta cgttgctgat cttccagacg
    15361 cttcgccagc accgccagat cttctttcgg atcatcgccc atgatgacga tgactgcttt
    15421 ctgcccggtg gcgtcggacc agacatgcat gttattggcc tgcgttccca gcttaccgct
    15481 ctggtcggtc atatccgctg gcagcgagaa acttaacttg ccatcaagca ggttgacggg
    15541 attcccggta gcgttatttt ccgcgaccga accctgcgcc gtagcgttag tgtctttatc
    15601 atcacaggcc gcaagcccca taaccagcag gccaattccg acatatttaa ccagattgcg
    15661 cattgacttc ttcctttcga taaacggcca taacggctca ttcatccatc ttatcacaac
    15721 tctgataacg aacctttaac tcgcctgcaa agcgttgatt tcggatttat ctgccagtct
    15781 tttcaacagc atattgagta atacgccata cattggcagg aagaaaacga tactgattaa
    15841 cactttgaaa cagtaatcga ccagcgcgat ttccatccag tgttcagcca taaaggcatc
    15901 cgggctacgc cagaaggcaa tgaagaaaaa ggccagcgtg tcgctgacgt taccgaacag
    15961 tgtggacgct gtcggtgcca gccaccagcg gcgactctga cgcaggcggt taaaaacgtg
    16021 cacgtcgagg atctgtccca gcgcgtaggc catgaaactg gcggtggcga tacgggcgac
    16081 aaacaggttg aagtgggcga gtgcgccgaa tccctgccag gaacccatat agaatagcga
    16141 cgagatgacg taggagatca gcaacgccgg gatcatcacc gcaaagatga tacgacgggc
    16201 cagcggtgcg ccaaaaatac gcacggtcag gtcggtagca aggaaaataa acgggaagct
    16261 aaacgcgccc caggtggtat ggaaacccaa aatggagacg ggaagctgaa ccagatagtt
    16321 actggaggtg atcaccagca gatgaaataa cgataaccag aacaacgcct tatagcgttg
    16381 agtttgcgag aaaacgttca tattgtacct ttttgattaa ccattggggt gagggaaccc
    16441 aatacgtacg acacgtctgt taccttaaca taataacgtg cttacccttt tttcgagccg
    16501 ccgcatgata ctgctttacg ttgacaatgc aatggttaat tttcacgcaa tcgttaacct
    16561 ggtttgctta cggactcaca gggcgtaaac tagcgccgtt tttttatgtg atgagaagaa
    16621 aatgaccgat ctcttttcca gccctgacca cacactcgac gcacttggcc tgcgctgccc
    16681 ggaacccgta atgatggtgc gcaaaactgt gcgcaatatg cagcccggcg aaacgttgct
    16741 gattatcgcc gacgatccgg ccactacccg cgatattcct gggttttgta cctttatgga
    16801 acacgaactg gttgcgaaag agacggatgg actgccttat cgttatttga ttcgtaaagg
    16861 cggttgatgg gggctgattg gcttcgatgc cgccttttcc cctcacccta accctctccc
    16921 cagaggggcg aggggaccga tcgcgctcaa tgttgcgatc ggtttgcctt atctcctgcg
    16981 caacaatctt aacgcattcg ctgtcaccag caccgtcgca cccgtatctg ccagcaccgc
    17041 cagccatagt ccggtcatcc ctaaaagcgt ggtgacgagg aagatccctt tcagtcccag
    17101 cgcaatggtg atgttctggc ggatattggc gtgagtggcg cgtgccagtt caatcatttg
    17161 caccaggccg cgcaggtggt tatgggttaa tgctgcatca gcggtttcca gcgccacgtc
    17221 tgtgccactg cccattgcaa tcccaatggc ggcagctttc atcgctggcg cgtcgttaat
    17281 accgtcaccg accatcgcca gcggcgcatg ttgattcagc tcggtcaccg ctttgacttt
    17341 atattccggc aacaggcccg ctttaaattc cagccccagc tccccggcaa ttgccgccgc
    17401 tgcgcgtgga ttatcgccgg tgaggatcac ccctttgacg cccagcgcgt tcagttcact
    17461 gatggcagtt gcagcatcgg cgcgcagggt atcctgcaat gcaatgatac ccagcacgtc
    17521 atcattacgt actaccagca caaccgtttg cccggcgctt tccagttcat taatcaaacc
    17581 agcaaatgca tcagcgggat gtttcccggc agcgcatatc aacacgcgct caccgttaac
    17641 ctgcgcttca atgccagacc cgaccagcgc ccgctgtgat tcggcggcgg gaatggcgag
    17701 tgcagcaacc tgtgcttcgc gcacgatggc ctgcgccagt ggatgcgtcg cgctttgctc
    17761 gaccgccgcc gccagtgtca gcagttcaga ttcactaata cccgttgccg gatgaatcgc
    17821 ggtaacgcgc ggtttaccga cggtcagcgt accggtttta tcaaacgcca cctgagtaac
    17881 acgacctagt tgttccagcg ccgctccgcc tttaatcaac gccccacgac gcgctgctgc
    17941 cgccagcccg gaggtaatcg ccgcgggcgt ggagataact aacgcacacg ggcagccaat
    18001 cagcagcagc gtcaggcctt tataaatcca ctcctgccag ctggcggcaa acagcagcgg
    18061 tggcaccagc gtcaccagca gggcgacggc cataatcgct ggcgtataaa tacggctgaa
    18121 acggtcgata aaccgctcaa tgggggcgcg gcgctcttcg gcttcttcga tcagtttcag
    18181 aatccggtca atggcgctgg cacccggttc tgacagcact tccagcgtta ccagacggtc
    18241 tacgctggtg gctcctgcag gaactttatc gcccgtcgcg cgctccaccg gaatggattc
    18301 tccggtcagg gcgctttcat caaaactggc aaacggtgag agcagtttac cgtcggcagg
    18361 caaacgccca cctgcggcga cttcaatcac atcgccaggc cgcaggctgt taatcgccac
    18421 ctcttcccgc tcaccgttac gcaggcgcgt ggcggtttct ggtttcagcg ccattaacgc
    18481 gctaacgccc tgacgcgcgc ggctggcggc ccagccttcc agtcgttcac caatcaaaaa
    18541 cagcagcaac accatcgcag cttcagccgt tgcgccaata aacagtgcac caatagcggc
    18601 tacgctcatt aaggtttcaa tggcgaagta gctgccggat ttgatcagcc gtaatgcctg
    18661 atgagcaatc gggtacagcc caaccagcgt ggtcgcgata aacgccagtt gcccgaacgg
    18721 atgattgaac tgctccagac cccagctgat tgccatcatc acgattagtg taatcagcgg
    18781 cagattctct ttcaggcgcg atgcttgtgg ctcgtcggcg gcctgctcat cgcgcaggga
    18841 atagcccgct ttttgcaccg cagattcaac ttgtgcacga atgtcattgt cggcatcaac
    18901 caccagtttt tcggtggcga acaacacctg cacctgattc acacctgcaa gctggcgcac
    18961 ggcattttct accttgcgcg cacaggcggc gcagtccatg ccactgactt tccagctata
    19021 gcgggtgccg gagacgtttt cagagagcgt tggcgagctg gagcatgcgc cgtcgcagca
    19081 acagtcgttg gcgttctgta ccgtggttag cggtttgaac gcagcaaatt gaggggcttt
    19141 cttgccgtga ttgtcaggtg tcgacatggc atactccggt taagtttttt ctcattaacc
    19201 gaaggataca ctctggagtc gactccagag tcaagtttta tcagaaatac agtgagcgga
    19261 cgatgaggaa gtgaccggca aagtagcagg ccgcagcaat cgcgttatcc gcacggaagc
    19321 gtcggcgata gtggctcccc agccagacaa agttactgat aaacagcaac gaagcgccaa
    19381 caaacgcaga gagcgccgga gcggtcggac ggaagaacca cagttcacct gccagccaca
    19441 ccatcaccag cgtcatgccg ataaacgtgc agataggcca acgatactct tccagccgcg
    19501 tccagataat cgccagtaac agcgcaccca gaaccagcaa caccagtggc agaggccaga
    19561 agaaagagag cgtcatctga ctggcgaaat agatggtgta cagcaggtgc gagaggaaaa
    19621 acgcgccgat ggcgtacatc agacgttgac gtggcaacag ggttagcgca tcgcccagca
    19681 gtgaggcgca cagccctgcc agcaccagat agctaatggc gtcgaacatc ggcgcttgcc
    19741 aggccagtaa cagcaggaga agaagggtta acggtttaaa cacccagcgt tgccaggttg
    19801 gcccacgata cgatgcatcc acagatagcc atgcggaaag acagacagcg ataaacgacc
    19861 aaagcatctt agttccttga attcgttatt ttttcgtaag cggaatgcct acggtcttgc
    19921 ttccagttta gtggcgaggg tggacggatg acaacaccat aactagcagg gtatgcttat
    19981 ttccgcattt tccgatgagg gataaagatg agcaaaccac ctcttttctt tattgttatc
    20041 attggcttaa ttgtcgtcgc cgcatcgttt cgttttatgc aacagcgacg ggaaaaagct
    20101 gataatgata tggctccgct ccagcaaaag ctggtggtgg tgagcaacaa gcgggaaaaa
    20161 ccgattaacg atcgccgttc gcgccagcag gaagtgactc cggcaggcac cagtatgcgc
    20221 tatgaggcaa gcttcaaacc gcaaagcgga ggaatggagc agacgtttcg cctcgacgcc
    20281 cagcagtacc atgccctgac agtgggcgat aaaggtacgc tgagctataa aggaacgcgc
    20341 tttgttagct ttgttggcga acaataaggt tattttttaa ctttaaattt cttctgccag
    20401 accagcagtt caaacacgcc gaaaaggaaa atccgcacct tttcggcggt ggtcatctgc
    20461 gggccatctt tcggtaaagt ggatttcaat agcgccagct gcataccatg catcagcacg
    20521 gtaaaaatca gcgccacgtt aacgaaaata ttcagcgggc gcgggaaggg atgcaccaga
    20581 ttgaggatta aaaatcccca aacgcagagc attaacaaac gaccaatatt aatcagcatc
    20641 actttctcct tgtgcttcgc gttgatacag ccgataagcc acctgacccg ccactttttc
    20701 ccgatgtaat gaccagtttg ccggaacagt gggcagaccg ttttcgactt cgctttcgac
    20761 ataaatcagg gcttcgtcag ccagccagcc gttatcttcc agtaaattta tcgtctcttc
    20821 taacaagcca cggcggaacg gtggatcgac aaacacgata ttatgcggtg tacctttttg
    20881 cgccaggaat gacatcgcgt tgctgttcac cacgcgtgca ttgcctgctt ttagtgtcgc
    20941 cagattctta attaactgct gagaaaccgc gcgatccatc tcaatcaacg tggcccccgc
    21001 agcgtagcgc gataacgctt ccagtcccag cgcgccgctc ccggcgaagc aatccagaca
    21061 ttgggcgtca acaatgaccg gagccagcca gttaaacaat gtttcgcgta cgcggtcggt
    21121 ggtggggcgc agacctgggc tatcaggaac cgggagttta cggcctcgcc actgcccgcc
    21181 aataatgcgg atttggccgc tgccggaatg attcggtttt ttcatgataa attgctcaat
    21241 ccgccagata acacatactt gcaggcggtg atgtgaatta agttaagtga tagactattt
    21301 catcattttt ttagctgcta tgtacatagc gttaacgctg tgccatgaag caacagcgag
    21361 gagtgtagtc gcaaatggcg aaagaaaaaa aacgtggctt tttttcctgg ctgggctttg
    21421 gtcaaaaaga gcagaccccg gaaaaagaga cagaagttca gaatgaacaa ccggttgtag
    21481 aagaaatcgt tcaggcgcaa gagcctgcga aggcttctga acaagccgtc gaagagcaac
    21541 cgcaggcgca tactgaagcc gaggcggaaa cttttgctgc cgacgttgtg gaagtcactg
    21601 aacaggttgc tgaaagtgaa aaagcgcagc ctgaagcgga agtcgttgca cagccggaac
    21661 cggtcgtaga agaaacgccg gagccagtgg ctatcgaacg tgaagagctg ccgttgccgg
    21721 aagacgtcaa cgccgaagag gtttcgccag aagagtggca ggctgaagcg gaaaccgtag
    21781 agattgtcga agcggcggaa gaagaagcgg ctaaagaaga aatcaccgac gaagagctgg
    21841 aagcacaggc gctggctgcc gaagcggcag aagaggcggt gatggtggtt cctccggtag
    21901 aagagcagcc ggtggaagaa atcgctcagg agcaggaaaa accgaccaaa gaaggtttct
    21961 tcgcgcgcct gaaacgcagc ctgttaaaaa ccaaagaaaa tctcggttcc ggatttatca
    22021 gcctgttccg cggtaaaaaa atcgacgatg atctgtttga ggagctggaa gaacagctgt
    22081 tgatcgccga tgtgggcgtg gaaaccacac gtaaaattat caccaatctg acggaaggcg
    22141 catcccgcaa gcagcttcgt gatgccgagg cgctctatgg cctgctgaaa gaagagatgg
    22201 gcgagattct ggcgaaagtc gatgagccgc tgaatgttga aggtaaaacg ccgttcgtga
    22261 tcctgatggt gggcgtcaac ggtgtgggta aaactacgac gattggtaag ctggcacgtc
    22321 agtttgagct gcagggtaaa tcggtgatgc tggcggcggg cgatactttc cgtgcagcag
    22381 cggttgaaca gcttcaggtc tggggtcagc gcaacaatat tccggtgatt gcccagcata
    22441 ccggtgcgga ttccgcctct gttatcttcg acgccattca ggcagctaaa gcgcgtaata
    22501 tcgacgtcct gattgccgat accgccggac gcctgcagaa caaatcgcac ctgatggaag
    22561 agttgaagaa aatcgtccgc gtgatgaaga aactcgacgt tgaagcgccg catgaagtta
    22621 tgctgactat tgatgccagt accgggcaga atgcggtaag ccaggccaaa ctgttccacg
    22681 aagccgttgg cttaaccggc atcacgctaa cgaaactgga cggcacggcg aaaggcgggg
    22741 taattttctc ggtggctgac cagtttggta tccctatccg ctacattggt gtcggcgaac
    22801 gtattgagga tttgcgtccg tttaaggcgg acgactttat agaggcactt tttgcccgag
    22861 aggattaaca atgattcgct ttgaacatgt cagcaaggct tatctcggtg ggagacaggc
    22921 gctgcagggc gttacgttcc atatgcagcc gggtgagatg gcgtttctga ccggtcattc
    22981 cggcgcaggg aaaagtaccc tcctgaagct gatctgtggg attgagcggc ccagcgccgg
    23041 gaaaatctgg tttagcggcc atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct
    23101 gcgccgccag attggcatga ttttccagga tcaccatcta ctgatggacc gtactgtcta
    23161 cgataacgtg gcgatcccgc tgattatcgc tggtgccagc ggtgacgata ttcgtcgccg
    23221 ggtgtcggcg gcactggata aagtcgggct actggacaaa gcgaagaact tccctattca
    23281 gctttccggc ggtgaacaac agcgtgttgg cattgcccgc gcggtggtga ataagcccgc
    23341 ggtactgctg gcggacgaac cgaccggtaa cctggacgac gcgttgtcgg aagggattct
    23401 acgtctgttt gaagagttta accgcgttgg ggtaaccgta ttgatggcaa cgcacgacat
    23461 caacctgatt tcacggcgtt cctatcgcat gctcaccctg agcgatggtc acttgcatgg
    23521 aggcgtgggc catgaataag cgcgatgcaa tcaatcatat tcggcagttt ggtgggcgtc
    23581 tggatcgctt ccgtaaatcg gtcggcggct caggcgacgg cggtcgtaac gcaccgaaac
    23641 gcgcgaaatc ctcgccaaaa ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc
    23701 gctatgcctt ccacggcgca ttgcaggatc tgaaaagcaa accgttcgcc acgtttttaa
    23761 cggtgatggt tatcgccatc tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa
    23821 acgttaatca ggcggcgacg cagtattatc cgtcaccgca aatcactgtt tacttgcaaa
    23881 aaacgctgga cgatgacgcc gctgcgggcg tggtggcaca gttgcaggcc gagcaaggcg
    23941 tggagaaagt gaactatctt tctcgtgaag acgcactggg cgagttccgt aactggtctg
    24001 gttttggtgg tgcgctggat atgctggaag aaaacccgct tccggcagtg gcggtggtga
    24061 tcccgaaact cgattttcag gggacggaat cactcaatac gctgcgtgat cgtatcacgc
    24121 agattaacgg cattgacgaa gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt
    24181 tgaccgggct ggtcgggcgc gtttcggcga tgatcggcgt gttgatggtg gcggcagtgt
    24241 tcctcgtcat cggcaacagc gtacgtctga gcatctttgc tcgccgtgac tccatcaacg
    24301 tgcagaaact gattggtgcg acggatggtt tcatccttcg cccgttcctg tatggtggcg
    24361 cactgctggg attttctggc gcattgttgt cattaatttt gtcagaaatt ctggtgctgc
    24421 gattgtcatc ggcggttgcg gaagtggcac aggttttcgg aacgaatttt gatatcaatg
    24481 gtttatcgtt cgatgaatgc ctgctattgc tgctggtatg ctcgatgatt ggctgggtgg
    24541 cagcgtggct tgccacggta caacatttac gccactttac gcctgaataa taaaagcgtg
    24601 ttatactctt tccctgcaat gggttccgta gcagggaaag agatcccgtt gtctcttccc
    24661 ggtatttcat ctctatgtca cattttgtgc gtaatttatt cacaagcttg cattgaactt
    24721 gtggataaaa tcacggtctg ataaaacagt gaatgataac ctcgttgctc ttaagctctg
    24781 gcacagttgt tgctaccact gaagcgccag aagatatcga ttgagaggat ttgaatgact
    24841 gacaaaatgc aaagtttagc tttagcccca gttggcaacc tggattccta catccgggca
    24901 gctaacgcgt ggccgatgtt gtcggctgac gaggagcggg cgctggctga aaagctgcat
    24961 taccatggcg atctggaagc agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt
    25021 catattgctc gtaattatgc gggctatggc ctgccacagg cggatttgat tcaggaaggt
    25081 aacatcggcc tgatgaaagc tgtgcgccgt ttcaacccgg aagtgggtgt gcgcctggtc
    25141 tccttcgccg ttcactggat caaagcagag atccacgaat acgttctgcg taactggcgt
    25201 atcgtcaaag ttgcgaccac caaagcgcag cgcaaactgt tcttcaacct gcgtaaaact
    25261 aagcagcgtc tgggctggtt taaccaggat gaagtcgaaa tggtggcccg tgaactgggc
    25321 gtaaccagca aagacgttcg tgagatggaa tcacgtatgg cggcacagga catgaccttt
    25381 gatctgtctt ccgacgacga ttccgacagc cagccgatgg caccggtgct ctatctgcag
    25441 gataaatcat ctaactttgc cgacggcatc gaagatgata actgggaaga gcaggcggca
    25501 aaccgtctga ccgacgcgat gcaaggtctg gacgaacgca gccaggacat catccgcgcg
    25561 cgctggctgg acgaagacaa caagtccacg ttgcaggaac tggctgaccg ttacggtgtt
    25621 tccgctgaac gtgtacgcca gctggaaaag aacgcgatga aaaaactgcg cgctgctata
    25681 gaagcgtaat ttccgctatt aagcagagaa ccctggatga gagtccgggg tttttgtttt
    25741 ttgggcctct acaataatca attccccctc cggcaaaacg tcaatcccca cgcagattgt
    25801 taataaactg tcaaaatagc tattccaata tcataaaaat ggggtatgtt ttagcagagt
    25861 atgctgctaa agcacgggta gtcatgcata aaacgaaata aagtgctaaa tcacaacacc
    25921 acaacacacg taataaccag aagaatgggg attctcagga tgaatataaa gggtaaagcg
    25981 ttactggcag gatgtatcgc gctggcattc agcaatatgg ctctggcaga agatattaaa
    26041 gtcgcggtcg tgggcgcaat gtccggtccg gttgcgcagt acggtgacca ggagtttacc
    26101 ggcgcagagc aggcggttgc ggatatcaac gctaaaggcg gcattaaagg caacaaactg
    26161 caaatcgtaa aatatgacga tgcctgtgac ccgaaacagg cggttgcggt ggcgaacaaa
    26221 gtcgttaacg acggcattaa atatgtgatt ggtcacctct gttcttcatc aacgcagcct
    26281 gcgtctgata tctacgaaga cgaaggcatt ttaatgatca ccccagcggc aaccgcgccg
    26341 gagctgaccg cccgtggcta tcagctgatc ctgcgcacca ccggcctgga ctccgaccag
    26401 gggccgacgg cggcgaaata tattcttgag aaagtgaaac cgcagcgtat tgctatcgtt
    26461 cacgacaaac agcaatacgg cgaaggtctg gcgcgagcgg tgcaggacgg cctgaagaaa
    26521 ggcaatgcta acgtggtgtt ctttgatggt atcaccgccg gggaaaaaga tttctcaacg
    26581 ctggtggcgc gtctgaaaaa agagaatatc gacttcgttt actacggcgg ttatcacccg
    26641 gaaatggggc aaatcctgcg tcaggcacgc gcggcagggc tgaaaactca gtttatgggg
    26701 ccggaaggtg tggctaacgt ttcgctgtct aacattgcgg gcgaatcagc ggaagggctg
    26761 ctggtgacca agccgaagaa ctacgatcag gttccggcga acaaacccat tgttgacgcc
    26821 atcaaagcga aaaaacagga cccaagcggc gcattcgttt ggaccaccta cgccgcgctg
    26881 caatctttgc aggcgggcct gaatcagtct gacgatccgg ctgagatcgc caaatacctg
    26941 aaagcgaact ccgtggatac cgtgatgggt ccgctgacgt gggatgagaa aggtgatctg
    27001 aaaggctttg agttcggcgt atttgactgg cacgccaacg gcacggcgac cgatgcgaag
    27061 taatcattaa tcggcaactt tgggttgccg ccaaattgct aatatcgagt acgttgcttc
    27121 atgccggatg cggcgtaaac gccttatccg gcctacaaga tccaaagaaa tcagtaaatt
    27181 gcaacacaca ttgtaggcct gataagcgta gcgcatcagg caatttctcg ttggtcatca
    27241 cttttgaaat caacttgacg attaacgctt ctcccagccg ccctgttgcg ccgtaaatcc
    27301 cagtgcctgc ataaacgccg tcatcacacc gcgatcttcc acgcctgcat ccgccatcca
    27361 ccagcatgaa acgccaggat tgttacgcaa aacctcttcc agcagatatt gccccacacc
    27421 gcgacgtcgg gtgacttccc gcacacgcag ggaatccagt gctccctcgg tgccgcttaa
    27481 ggttacccgt acggcagcga gcaggcgctc gttaaaacgc gcggcgtaga tacggtggtt
    27541 atcgtcaacc tgtaaagagg aaggggaata ctccggccag atcttttgca ggtcaatccg
    27601 gtcctggtcg ctaaagtttt ctaatcgaat gatggtcagc ttcatgggta acccgtgtaa
    27661 atcacaaaag tggaaccagt gtagcgaaat aatttaatcg gaggctttct cttttttatt
    27721 tcttttggca ggtgattaat tttttaacag caataattac aaagttaaaa cattagagaa
    27781 tgaaaaatac ccagcataat cccctgaatg gtagtgaatt attccgccct ttgtgccgtt
    27841 at
//
LOCUS       DAJVOY010000036        27611 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVOY010000036 DAJVOY010000000
VERSION     DAJVOY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.036"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(146..355)
                     /gene="fumD"
                     /locus_tag="OQL90_001375"
     CDS             complement(146..355)
                     /gene="fumD"
                     /locus_tag="OQL90_001375"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS1368205.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELDKQAMETVINALVK"
     gene            912..2324
                     /gene="pykF"
                     /locus_tag="OQL90_001376"
     CDS             912..2324
                     /gene="pykF"
                     /locus_tag="OQL90_001376"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1368206.1"
                     /translation="MKKTKIVCTIGPKTESEEILAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2635..2871
                     /gene="lpp"
                     /locus_tag="OQL90_001377"
     CDS             2635..2871
                     /gene="lpp"
                     /locus_tag="OQL90_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1368207.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(2935..3939)
                     /gene="ldtE"
                     /locus_tag="OQL90_001378"
     CDS             complement(2935..3939)
                     /gene="ldtE"
                     /locus_tag="OQL90_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1368208.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(4088..4504)
                     /gene="sufE"
                     /locus_tag="OQL90_001379"
     CDS             complement(4088..4504)
                     /gene="sufE"
                     /locus_tag="OQL90_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1368209.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(4517..5737)
                     /gene="sufS"
                     /locus_tag="OQL90_001380"
     CDS             complement(4517..5737)
                     /gene="sufS"
                     /locus_tag="OQL90_001380"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1368210.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(5734..7005)
                     /gene="sufD"
                     /locus_tag="OQL90_001381"
     CDS             complement(5734..7005)
                     /gene="sufD"
                     /locus_tag="OQL90_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1368211.1"
                     /translation="MAGLPNSSNALQQWHHLFDAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(6980..7726)
                     /gene="sufC"
                     /locus_tag="OQL90_001382"
     CDS             complement(6980..7726)
                     /gene="sufC"
                     /locus_tag="OQL90_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1368212.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(7736..9223)
                     /gene="sufB"
                     /locus_tag="OQL90_001383"
     CDS             complement(7736..9223)
                     /gene="sufB"
                     /locus_tag="OQL90_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1368213.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(9232..9600)
                     /gene="sufA"
                     /locus_tag="OQL90_001384"
     CDS             complement(9232..9600)
                     /gene="sufA"
                     /locus_tag="OQL90_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1368214.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(10147..10335)
                     /locus_tag="OQL90_001385"
     CDS             complement(10147..10335)
                     /locus_tag="OQL90_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1368215.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(10435..10845)
                     /gene="menI"
                     /locus_tag="OQL90_001386"
     CDS             complement(10435..10845)
                     /gene="menI"
                     /locus_tag="OQL90_001386"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1368216.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(10842..13898)
                     /locus_tag="OQL90_001387"
     CDS             complement(10842..13898)
                     /locus_tag="OQL90_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1368217.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            14287..15399
                     /gene="ydiK"
                     /locus_tag="OQL90_001388"
     CDS             14287..15399
                     /gene="ydiK"
                     /locus_tag="OQL90_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1368218.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            15585..15692
                     /gene="rprA"
                     /locus_tag="OQL90_001389"
     ncRNA           15585..15692
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQL90_001389"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            15828..16207
                     /locus_tag="OQL90_001390"
                     /pseudo
     CDS             15828..16207
                     /locus_tag="OQL90_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            16152..16874
                     /locus_tag="OQL90_001391"
                     /pseudo
     CDS             16152..16874
                     /locus_tag="OQL90_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16886..17752
                     /gene="ydiB"
                     /locus_tag="OQL90_001392"
                     /pseudo
     CDS             16886..17752
                     /gene="ydiB"
                     /locus_tag="OQL90_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            17784..18542
                     /gene="aroD"
                     /locus_tag="OQL90_001393"
     CDS             17784..18542
                     /gene="aroD"
                     /locus_tag="OQL90_001393"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1368219.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18688..20283
                     /locus_tag="OQL90_001394"
     CDS             18688..20283
                     /locus_tag="OQL90_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCS1368220.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            20297..21448
                     /locus_tag="OQL90_001395"
     CDS             20297..21448
                     /locus_tag="OQL90_001395"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCS1368221.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21491..22402)
                     /locus_tag="OQL90_001396"
     CDS             complement(21491..22402)
                     /locus_tag="OQL90_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1368222.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            22716..23487
                     /locus_tag="OQL90_001397"
                     /pseudo
     CDS             22716..23487
                     /locus_tag="OQL90_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            23507..24445
                     /locus_tag="OQL90_001398"
     CDS             23507..24445
                     /locus_tag="OQL90_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCS1368223.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLVATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24501..25790
                     /locus_tag="OQL90_001399"
     CDS             24501..25790
                     /locus_tag="OQL90_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1368224.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25787..26080
                     /locus_tag="OQL90_001400"
     CDS             25787..26080
                     /locus_tag="OQL90_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1368225.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            26137..>27611
                     /gene="fadK"
                     /locus_tag="OQL90_001401"
     CDS             26137..>27611
                     /gene="fadK"
                     /locus_tag="OQL90_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCS1368226.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSL"
BASE COUNT         6884 a         6993 c         6795 g         6939 t
ORIGIN      
        1 cagaaaataa aaataatatt atcgttataa attaaaatac ataccgcaat tcgtataatt
       61 tcagcagttg ttatttagaa tgattaactg gaccgcaact gaagcaagaa tatcaggatg
      121 aagcttaccg cctcatcctg aatggtcatt ttaccagtgc gttaatgacg gtttccatcg
      181 cctgcttatc cagctcactg cgctgaatgc gtttattggc taatgctgta cgtaatacgc
      241 ctgcgataac aatatgcttt ggttcctgcc ccagatcgcg catttccagc acaactttac
      301 cgacaactct gcacatttct cggtacagtt catcctcttt tgtccgattt cccattcttt
      361 atacctattt atcatgccaa ctatcagcat atatcaatct aaccaattaa acaaaaatca
      421 aacaaaatca gacaaataac gcgataaatt attttaactg tagcaattga gcgatgatat
      481 atttatacac cggatgaact ttcacttatc ctcacactga caactttggc accagacgtt
      541 gcgcaaacag cgaagttttt gcgtaacctt ttccctggaa cgttaaatct ttgataacaa
      601 tttattgtct aacaagttgt atattttttg aaacgctgtt tttgttttcc ttttggatta
      661 atttcagcgt ataatgcgcg ccaattgact cttgaatggt ttcagcactt tggactgtag
      721 aactcaacga ctcaaaaaca ggcactcacg ttgggctgag acacaagcac acattcctct
      781 gcacgctttt tcgatgtcac ctatccttag agcgaggcac caccactttc gtaataccgg
      841 attcgctttc cggcagtgcg cccagaaagc aagtttctcc catccttctc aacttaaaga
      901 ctaagactgt catgaaaaag accaaaattg tttgcaccat cggaccgaaa accgaatctg
      961 aagagatatt agctaaaatg ctggacgctg gcatgaacgt tatgcgtctg aacttctctc
     1021 atggtgacta tgcagaacac ggtcagcgca ttcagaatct gcgcaacgtg atgagcaaaa
     1081 ctggtaaaac cgccgctatc ctgcttgata ccaaaggtcc ggaaatccgc accatgaaac
     1141 tggaaggcgg taacgacgtt tctctgaaag ctggtcagac ctttactttc accactgata
     1201 aatctgttat cggcaacagc gaaatggttg cggtaacgta tgaaggtttc actactgacc
     1261 tgtcagttgg caacaccgta ctggttgacg atggtctgat cggtatggaa gttaccgcca
     1321 ttgaaggtaa caaagttatc tgtaaagtgc tgaacaacgg tgacctgggc gaaaacaaag
     1381 gtgtgaacct gcctggcgtt tccattgctc tgccagcact ggctgaaaaa gacaaacagg
     1441 acctgatctt tggttgcgaa caaggcgtag actttgttgc tgcttccttt attcgtaagc
     1501 gttctgacgt tatcgaaatc cgtgagcacc tgaaagcgca cggcggcgaa aacatccaca
     1561 tcatctccaa aatcgaaaac caggaaggcc tcaacaactt cgacgaaatc ctcgaagcct
     1621 ctgacggcat catggttgcg cgtggcgacc tgggtgtaga aatcccggta gaagaagtta
     1681 tcttcgccca gaagatgatg atcgaaaaat gtatccgtgc acgtaaagtc gttatcactg
     1741 cgacccagat gctggattcc atgatcaaaa acccacgccc gactcgcgca gaagccggtg
     1801 acgttgcaaa cgccatcctc gacggtactg atgcagtgat gctgtctggt gaatccgcaa
     1861 aaggtaaata cccgctggaa gcggtttcta tcatggcgac catctgcgaa cgtaccgacc
     1921 gcgtgatgaa cagccgtctc gagttcaaca atgacaaccg taaactgcgc attaccgaag
     1981 cggtatgccg tggtgccgtt gaaactgctg aaaaactgga tgctccgctg atcgtggttg
     2041 ctactcaggg cggtaaatct gctcgcgcag tacgtaaata cttcccggat gccaccatcc
     2101 tggcactgac caccaacgaa aaaacggctc atcagttggt actgagcaaa ggcgttgtgc
     2161 cgcagctggt taaagagatc acttctactg atgatttcta ccgtctgggt aaagaactgg
     2221 ctctgcagag cggtctggca cacaaaggtg acgttgtagt tatggtttct ggtgcactgg
     2281 taccgagcgg cactactaac accgcatctg ttcacgtcct gtaatattgc ttttgtgaat
     2341 taatttgtat atcgaagcgc cctgatgggc gcttttttta tttaatcgat aaccagaagc
     2401 aataaaaaat caaatcggat ttcactatat aatctcactt tatctaagat gaatccgatg
     2461 gaagcatcct gttttctctc aattttttta tctaaaaccc agcgttcgat gcttctttga
     2521 gcgaacgatc aaaaataagt gccttcccat caaaaaaata ttctcaacat aaaaaacttt
     2581 gtgtaatact tgtaacgcta catggagatt aactcaatct agagggtatt aataatgaaa
     2641 gctactaaac tggtactggg cgcggtaatc ctgggttcta ctctgctggc aggttgctcc
     2701 agcaacgcta aaatcgatca gctgtcttct gacgttcaga ctctgaacgc taaagttgac
     2761 cagctgagca acgacgtgaa cgcaatgcgt tccgacgttc aggctgctaa agatgacgca
     2821 gctcgtgcta accagcgtct ggacaacatg gctactaaat accgcaagta atagtacctg
     2881 tgaagtgaaa aatggcgcac attgtgcgcc attttttttg tctgctattt accactactg
     2941 cctcgcgcgt aacatattcc cttgctctgg ttcaccattc tgcgctgact ctactgaagg
     3001 cgcattgctg gctgcgggag ttgctccact gctcaccgaa accggatacc ctgcccgacg
     3061 atacaacgct ttatcgacta acttctgatc tacagcctta ttgtctttaa attgcgtaaa
     3121 gcctgctggc agtgtgtatg gcattgtctg aacgttctgc tgttcttctg ccgatagtgg
     3181 tcgatgtact tcaacataac gcatcccgtt aggctccacg gaatatttca ccggttcgtt
     3241 gatcactttc accggcgttc ccgtccgcac gctggagaat aaggctttaa tatccggtgc
     3301 attcatgcga atacagcccg aactgacgcg taaaccgacg ctgtccggcg cactggtacc
     3361 atgaatgagg tattcgccat taccatgcgc gaagcgcagt gcgtaacgtc ctagcgggtt
     3421 atttggtccg gcaggaacga ctggcagtaa tttaatgcca cgctccagcg aacgttgacg
     3481 aatgcctgcc gtaggcgtcc aggttggatt agggattttc tgccccacac gcgtttccat
     3541 caccggcgtt tccagcccct gcaagccaat acctatcgga taaacctgca cgatattttc
     3601 tcccggcgga taataataaa ggcgcagctc tgcaaggtta acgataatcc cctgacgcgg
     3661 tgcatcaggt aataacagtt gtgaaggaat agttatcgtc gtaccaggtt ttggcaccgg
     3721 ggcgatagtg ttattggctt caaggatcaa cattgccgca gtatcaaaac gtcgggcaat
     3781 agcctgaagg tttttatccc cttcttgcac cgtatacgtt tgattttgcc caaccagtcg
     3841 gcttccggtt ggtggtagcg gataatcaac cgcccaggca gcctggatgg cgctaaaagc
     3901 gccgataagc gtgagtgtaa gcaaagacgc gcgtttcatt gtaaacctcc tgtatttgcc
     3961 ggagactcac gctgaaacgt cggatggcgc ttatgttcac ctgaaaccaa aacactcctg
     4021 tgcaggtcag tgtaaacatt gaccatccgg caatgtgagc caaccggatg aaagctgtcc
     4081 ctttagttta gctaagtgca gcggctttgg cgcgaattgc gcgaatcatc gcttccagac
     4141 cttgtgaacg agatggggtg agatgttggg tgagcgccat tttttcaaac cacggacgca
     4201 catcgaaatt gacaatatcc tgcggcgtca tctgatcgta gagaataaat acgaccgcaa
     4261 taagcccttt cacaatcgcc gcatcgctgt cgccctgcaa ttcaataatt ccctgcgcat
     4321 tctggcgcat gacaatccac acctgattct ggcagccctg aatgctattt tgtgggcttc
     4381 ggtcttcgtc gcgtaattct ggcagacgct ggcccagctc aataatgtag agatatttct
     4441 cttcccagtt ggcgcagcgt aaaaaattac gcagcaattt ttctttatcc ggcaataaag
     4501 ccatagtgcc tccctgttat cccagcagac ggtgaatacg ttgcaggccg gtcaccagac
     4561 gatccacttc ttcatgagtg ttatacatgg ccagcgacgc ccgacacatc gcagggacgt
     4621 tgtaataggc cattaatggc atagcgcagt gatgtccggt acgcacagca atgccgtaat
     4681 tatcgagaaa actgccaaca tcataggcgt ggtgtttacc gagattaaaa gcaataacgc
     4741 caagcctgtt ttgtgggcca tagagagtga gattcggtac agattccagc tgtgatagcg
     4801 cgtaatgcat cagattcagt tcatactcgg ctatgttatt aagccccagc accgaaacat
     4861 attccagcgc cgcgccaaga ccaatgatgc ccccggtatt gggtgtaccg gcttcaaacc
     4921 gccatggtgc tttggtccag gtagtgcctt cactcaggct gacggtggcg atcatagaac
     4981 cgcccccttc ccacggcggc atctcctgca acaaggcttc tttgacataa agaatgccaa
     5041 tcccggtggg gccatacagt ttatgcccgg aaaacacgta aaaatcgcaa tccagcgcct
     5101 gaacatccac cggatgatgc atcaccgcct gagcgccatc caccagcact tttgcgccat
     5161 gctggtgcgc aagcgtgatc attcccgcca gtggattttc tgtgccaagc acgttggaga
     5221 catgagtaat tgccagcagg cgagttttct catcaaacag cgtatgcagc gtctccagtt
     5281 gcagcgtacc gtcggggttg agagggatca cacgcagctc tgcgccaacg cgtgcgcaaa
     5341 gcatctgcca ggggacaatg ttagcgtggt gctccatctg actgatgatg atgttatcgc
     5401 ccgcccgcac attgctgttg ccccagctat tggcgaccag attgatccct tccgtcgtgc
     5461 cgcggacgaa caccagctct tccgccgaac gggcattaat aaacagcgat gcccgcttgc
     5521 gcacgttctc cattttctcg gtcgcttggg cgcttaaggt atgaataccg cgatgcaccg
     5581 ccgcgtagcc atgacgataa aactcggcct cggcgtcaat cacctggctc ggtttctgtg
     5641 cactggcggc gctgtcgaga taagccagcg gcaaaccgtt tacctcacgc gaaagcaccg
     5701 gaaagtcggc ccgcactttg tcgacggaaa aagtcatctt gcacctcctg gcagccgttg
     5761 accgattcgg gccagcacct gctgtttaag cccctcatca cgcagtgctt ccgtcagttc
     5821 ggcagcaaag gcgtaaatga tcatctgctg ggcatcctgc tgattgatcc cgcgcgagcg
     5881 cagatagaac atctgttcat catcaatacg ccccaccgtc gcgccgtggc tgcatttcac
     5941 atcatctgca tagatttcca gctgcggttt cgtatccact tccgccagtt tgcccatcag
     6001 cagattattg ttggtcatct gaccatccgt tttgatggcg tgctgcgcga cgttgatcaa
     6061 accgttaaat accgcgcggc ctttgtcgct gacgatagtt ttgtgcaact gtcggctgtt
     6121 acaaaaacct ttattgtgtt ccagccaggt acgggtatca cacacctcgt ttttcaccgg
     6181 catcgccagg ctattgatcc gcagcgtgct gttttcgcca ttgagttgcg tactggtgtt
     6241 gtgtcgtaac actgcgccac ccagcaggaa actatggcta aatgcggtgg catcctcagc
     6301 cagcaacaaa tcgttatgag caaagtggtg actgagcggg ttttcaaacg ccagcttgat
     6361 atgctgcaag tgggcgttcg ctgcgacatt gatagtgaac cgtgccccgg taaaatgacg
     6421 agcatcatta aggctgacaa aatgttcgat caccgttgct tcggcacctt ccgccagatc
     6481 cagatgatgc cggtaatggg cagtgttcac ctcttcacct gccacgccct gggtgatata
     6541 cattaacagc aatggctttg ccggccgttg accgcgcttc acggcgatat gcgtcacgct
     6601 ttgtgccagg ctttccgtca aatgcagaaa cacttccgcc tgaatagcgt cgggtaaccc
     6661 ctgacggtcg tcgttaatgc tcacttcata tccgctgcct tcaattgcat cgctcagcgc
     6721 gggcacgtaa cgtccatcga caaacaccag ccgcacggcg tctaacgtta acgctaaggc
     6781 atcacgctgc tgtggggata tctcccccgc aatgctgaca aactggctat tggtcagccc
     6841 ttccagcgac gtatatttcc agttttcatg tttacgtgtc ggcagtccgg tacgcagcaa
     6901 ttgttgtaaa tgctgctgtg cttgcgggga gcgttttgtc ccttcagcgt caaacaagtg
     6961 atgccactgt tgcagcgcgt tactgctgtt cggtaagcca gccataaccc tgctcctcca
     7021 gttgtttgac caacgtgaaa tcgccggatt tcacaattcg tccctgatat agcacatgga
     7081 cgtaatcagg cttgatgtag tcgagaatgc gttggtagtg cgtaacaatg atgaatgagc
     7141 gcttgccatc acgcagggag ttcacgccat cggcgaccac ttttaatgcg tcaatatcca
     7201 gcccggagtc cgactcatca agaatgcata actccggttc cagcaccgcc atttgcaaaa
     7261 tatcgttgcg ctttttctcg ccgccggaaa aaccaacgtt taccgaacgg gttaataaat
     7321 cttccggcat cttcaggaga gcgattttct cttccatcaa atcctgaaaa tcaaagcggt
     7381 cgagcgtttc ctggccgcga tagctgcgca ctgcattaag tgccgtttgc aggaaaaact
     7441 ggttactgac gcctggaatc tccaccggat actggaaggc cataaagatg ccttcgcctg
     7501 cacgatcttc cggcgacagt gcaagcaaat ctttgccttt gaactcaacc gtgccgcccg
     7561 tcacttcata atcttcgcgc ccggcaagcg ttgccgataa ggtacttttg cccgaaccgt
     7621 ttggccccat aatggcgtga acttcgccgg gacgaacggc gaggcttaat ccacgcagga
     7681 tagctttatc ttccacgctg acgtgtaaat ctttaatact taacatgttt attccttatc
     7741 cgacgctgtg ttcaagactg atcgcgagga gtttttgtgc ttcaacggca aattccagcg
     7801 gcagttctga gaacacgtct ttgcagaaac cgttaacaat catcgagatg gcgtcttctt
     7861 cgctgatccc gcgttgcagg cagtaaaaca gttgatcttc accaatacgt gatgtcgttg
     7921 cctcgtgctc cagttgtgcg ctgttattgc gacattcgac atacgggaag gtatgcgccc
     7981 cacaattagc gccaatcagc attgagtcgc actgagtgaa attgcgcgca ttggttgccg
     8041 tcggcatgat tttcactaag ccgcgataac tgttctgact atgtccggca gagatccctt
     8101 tcgagataat ggtcgatttg gtgtttttac cgatgtggat catcttggtg ccggtatccg
     8161 cttgctgatg accgctggtc agcgccactg agtaaaactc accaatggag ttatcgccgc
     8221 gcaaaatgca gctgggatat ttccacgtaa tcgcggaccc ggtttctgat tgcgtccatg
     8281 acattttgct gttttcgcct tcgcacaaag cacgcttggt gacgaagttg agaataccgc
     8341 cggtgttgtt atcgccaggg aaccagtttt gtaccgtgga atatttcacc tcggcgtttt
     8401 tatggatgat gacttccacc actgccgcgt gtaactgata gctgtcacgc accggagcgg
     8461 aacagccttc aatgtagctg acgtagctgt cttcgtcggc caccagaatg gtgcgctcaa
     8521 actgcccggt tttttccgcg ttaatgcgaa aataggtgga aagttccatc gggcagcgca
     8581 cgcctttagg cacataaata aacgtaccgt cagaggctac cgccgcatta agcgcggcaa
     8641 agaagttgtc attccccggc accacggtgc cgagatattt acgcaccagt tccgggtgat
     8701 cgtggatcgc ctcaccaaag gaacagaaaa taattccctg ctccgccagt ttttcgcgat
     8761 aagtagtggc aaccgaaaca gagtcgaaaa tggcatccac cgccacctct ttgccttccc
     8821 gcacgggaac gcccaactgc tcaaacgccg cctccacctc tttacttaaa aaggcgttcg
     8881 cgccagtttg ctgcaccgcg ccaggttcag acgcgcaagt gtcgtcacaa ttaccgcacg
     8941 atggtgctga gtagtagctg taatcctgat aattcagctt gtcgtagtgc gctttcaacc
     9001 agtgcggttc ttccatctcc agccatgcgc gataggcgtt tagacgaaac tccagcatcc
     9061 actccggctc attacgcttc gccgaaattg cgcgcaccac ctcttcgttt atcccctttg
     9121 ccagctcatc ggtggctaac tgggtgaaga atccttcttt ataattcagc gggccgccgg
     9181 tccaggtttt gacatcgtca gttgcttcag tattacgaga catagtaccg cctatacccc
     9241 aaagctttcg ccacagccac attcattctg ggctttaggg ttgtgaaatt tgaatatctg
     9301 attaagtcct tcacgaacga aatcgacttc cgtgccatca ataaacggca tcgcttgcag
     9361 cgggacaaac agcttcgcgc cgtcgtgttc aaacagcaga tcgtctttgt ccggctcgct
     9421 aacactgtcg agcacatagc caaagcccgc gcagcccgtt tgcttcacgc ctaaacgcac
     9481 gccgaccata cccggctgct ttgccaccag ctcacggatg tgtatcgccg ctgcgggtgt
     9541 cagcgttaag ccttgccagg cgaaatcttg tgggttaaag gttcctgaat gcatgtccat
     9601 cgatttacct cacttcatcg ctttcagcgt ataacagcat gttggtgata atgattatca
     9661 gttcaaccca gcaaacgcag gggctttacc gtaaaacatg ctttttgcct gcttttaata
     9721 agcatagacc ctgatgtgtg ggttaacagg cacgctaaga attaggtatc tcattgttag
     9781 ataatggtta ttatctaagg agcattaagg ctgtgatgaa aaagaaaaag ttgtattaaa
     9841 atgactattt aagagatagg taaaaaagtg cagcgttcag aaataagaaa acccttaagt
     9901 ctgtgcgaca caggcttaag ggtttctacc ccatccggcg cttatctccg gcactctcag
     9961 tggcttagct cttgaagggg cgataagaat aatctcataa agctaacccg ccgttttaac
    10021 acaaactgcg attagtatta tttttgaaca atatcaggcg gtagataagc agtattaaga
    10081 aggtcatcga acctggacgg aggttaatcc aggtcgattt ggcgaacttg cggcattaag
    10141 tcaggatcaa tgcacgccca gacgccaggc aaagtagatt tcttctttta attcagcaga
    10201 agagagagta agcaggtcag caaattcaag ttctagttgt ttcagacgtt tgagatattg
    10261 ggcaggtgaa agattgctct ggtcacggcg taaaaattca atggccagct gggtgggatc
    10321 aagttgagta gacatagcat cctcgctttt agacaagacg tgtacagtat accaccgttt
    10381 actgtgcaga taatgaccaa aagcaatatg cgtcacactt ttctggtgac aacgtcacaa
    10441 aatggcggtc gtcaatcgtg acgaacagca caaacgccct ttctcatcga agatttcaat
    10501 ctgccagacc tggtgacgcg aaccgagatg caacggttta catatgccgc gcacccgccc
    10561 ttctcgtgcc gagcggacgt gattagcatt gatttccaga ccaaccactt tttgctcacc
    10621 ttcggtacat aaataaccgg caacggaacc gatactttcg gccagtacca cggatgctcc
    10681 tccatgcagc aacccgaaag gctgctttgt ccgcgagtct actggcattg tcgcttcaag
    10741 ggtgtcatca ccaatatgtt caaagcgaat atccaggaac cccaccatgt ttccttcacc
    10801 catagcattc agtgcttcca gggtgatttt ccgtttccat atcatttaat aatctccagt
    10861 aaagcctgca caggatggcg tacccccgtg ccttcaaccc gttttacctg gctacggcag
    10921 gaatatccgg tcgccagaca gcggttacgc ggcagtcgct gcatagcctg atgccaggat
    10981 aactcataga tcccgagcga attttcatgg tttttcgctt catgtccgta agtccctgcc
    11041 atgccgcagc aacccacgct gacattttcc agtttcgcgc caaaacgggc aaatatcgcg
    11101 gcccattgtg ctggcgcacc cggcaaggcg gtaacttcgg tacagtgacc aaagaaatac
    11161 catgattcac cgctgactgt agccacaggc tgtgactcaa gtgcgcttgc cagccattca
    11221 ttcgccagta agacgttaaa ctcgccacgc tcctcgccca gcgccagttt atattcatcg
    11281 cgataacaaa gtaccagcgc cggatcgacg cccaccattg gcatacccag cttcgccata
    11341 cggttgagga aatccgccgt ctttttcgcc gtcttcgcaa aacgattaag aaaaccttta
    11401 atatgctggg ctttgccatt tggcgaaaat ggcagtaaca caggctggaa acctaatttt
    11461 tcgaccagac ggacaaaatc cgccaccact tgcgcatcgt aatagctggt aaaggggtcc
    11521 tgcaccacca acactgtgcg cgctttctgc tctgcattga gagattcaag ctgttccagc
    11581 gtcatgtttg ccgagcgatg ccccaccatt tgttgttgta gcgaggggac cgacagcagc
    11641 ggcaaatcaa ccatgccgat atgtttttcc gagagtttgc gcaccagcgg ctggttaatg
    11701 aagaagttaa aggttttcgg cgcgcgtgcc atcagcggcg cgtagctctc gaccgtagcg
    11761 acgaggtggt cgcgcagcgg gcgtaaataa cgggtgtgat agagctgcag aaaacgagag
    11821 cgaaactccg gcacatcaat tttgatgggg cacagggtcg aacacgcttt acaggccaga
    11881 cagcccgaca tcgcctcttt gacttcgtgt gagaagtcat attcgccttt attcgcatgc
    11941 cagctattgc gcgtgcgggc aattaacgtc cgcaaactga cgccggattc aggcagttct
    12001 tgttccagtt tgagtggatc aacgccgcga tccgccaaca aacgcagcca ttcacgcacc
    12061 agcgttgcgc gccctttcgg tgaatgaatc cggttctggg tgatcttcat cgacggacac
    12121 atcggactac gggcatcaaa gttgaagcat aaaccgttgc cgttacactc catcgcaccg
    12181 cgccactgct ggcgtaccgc aatggggatc tgccgatcga atgtaccgcg cttcaccgcg
    12241 tccactttca tcatcggcgc atcgagacct tctggcgggc aaatcttccc tgggttgagt
    12301 cggttatgcg ggtcaaatgc cgctttcact ttgcgcagtt ctgcaaaaag ttcctcaccg
    12361 aaaaacgccg ggctgtattc agcgcgaaaa cctttgccgt gctcgcccca caacaaacca
    12421 ccgtatttcg cagtcagcgc caccacgtca tcagagattt gcttcatcaa aatctcttgt
    12481 tgaggatcgc acatatccag ctctggacgg acgtgcaaga cacctgcgtc gacgtgaccg
    12541 aacataccgt agcttaagcc gtggctgtcg agcagcgcgc gaaattcagc aatataatcc
    12601 gccaggtgtt ccggcggtac gcaggtatcc tcagcaaacg gaattggctt agcggcacct
    12661 ttggcattgc caagcagacc aacggctttt ttgcgcattg catagatacg ttcaaccccc
    12721 gccagctcac ggcacacctg ccagccgatg acacctgctt gatgactggc gatcagctca
    12781 tccaaccgcg cacagagtgc atttacccgc tcatcaatca gcgcctcatc atcaccagca
    12841 aattccacaa tgttcagccc gagcatctct tggtcaggca catcggtaat caactcgctg
    12901 acggaatgcc agacaatatc ttcccgcgcc agattcagca cttttgagtc caccgtctct
    12961 accgaaagcg cccgcgcctc aaccataaac ggcgcgttac gcagcgcaga gtcaaaagag
    13021 tcatatttga cgttcaccag acggcgcact ttaggcaagc gcgtaatatc cagccgcgct
    13081 tcggtaataa aggccagcgt cccttctgaa cccgtcagaa tgcgcgtcag gtcgaactcg
    13141 gtcatctcat cgttaaagac atgacgcaga tcgtaaccgg taagaaagcg gttaagtttg
    13201 gggaagttgt cgataattaa ctggcgttgc tgacggcaac gttgataaac cgtgttataa
    13261 attcgcccga ttgtggtatt ggatttaccc agcgtttccg ccaattcgac gggtaaaggt
    13321 tgcgtatcga gaacatcgcc ccccaacaac accgcgcgta cgccaagtac gtgatctgac
    13381 gttttgccat agaccagcga tccctgaccg gatgcatcgg tattgatcat cccaccgagc
    13441 gttgcccggt tgctggtcga aagttccggc gcaaaaaagt agccgaacgg tttcaggtac
    13501 tgattgagtt gatcttttat caccccggcc tcaacgcgca cccagccctc ttcagggtta
    13561 atttcgatga tgcggttcat atggcgggac atatcaacaa taatcccctg gttgagcgcc
    13621 tgaccgttag tgccggtgcc gccgccgcgg ggggtaaaga tcagcgatga atagcgttcc
    13681 tgcgcggcaa gacgggcgat cagcgccaca tctgcggttg aacgcggaaa taccaccgca
    13741 tcggggagaa gttggtaaat actgttgtcg gtcgacattg tcagacgatc ggcataactt
    13801 gtcgccgtat cgccggtaaa accttgttgc tccagctctt gcaaaaaatt aagcaccagt
    13861 tgaacgacgc cgggtgcctg agaaatctgt ggaatcatta tattgcccct ttcctgcggt
    13921 ctgtgatgta ggtcgataca ctattctttc aggcggctgc aatagcgcac tgaaaggtga
    13981 tgtttattta ctctatggat ttcgagttgc aggaaggcgg caagcgagtg aactccagga
    14041 gcttacaata gtaagtgact ggggtgaacg aacgtagccg cagcacatgc aacttgaaat
    14101 acgacgagta aatcgtttgc gtgttgcctg agttgttgta ccacattttt ttctaacacg
    14161 cccatcagaa ttaagggcag aatcggcctg ttaaaaaccg ctgaaattgc tcatcattat
    14221 gcaggtgagt ttcgcgtgtt cacgtcgcgt cgacgatttg acgcacaaaa aaggtgaaaa
    14281 gtagttatgg taaatgttcg tcagcccagg gatgtcgcac aaattctgct ttcggtgctg
    14341 tttttagcca tcatgattgt ggcatgtctg tggattgttc aaccctttat tctcggcttt
    14401 gcatgggccg gtacggtggt tatcgccacc tggccggtat tgttacgttt gcaaaagatc
    14461 atgtttggcc gccgctctct cgccgttctg gtgatgacgc tgttattagt gatggtgttt
    14521 atcatcccta ttgctttgct ggttaacagt atcgtcgacg gcagcggccc gctaattaaa
    14581 gccatttcca gcggcgacat gacgttaccc gatctggcgt ggcttaatac cattccggtg
    14641 attggcgcga agctgtatgc aggctggcac aacttgctgg atatgggggg gacggcgatc
    14701 atggcgaaag tccgccctta tattggcacc accaccacct ggttcgttgg gcaggcggcg
    14761 catatcgggc gctttatggt gcattgtgcg ctgatgcttc tcttcagtgc cctgctgtac
    14821 tggcgcggtg aacaggtggc acaaggcatt cgccattttg caacccgtct ggcaggcgtt
    14881 cgcggagatg ccgccgtact gctggcggca caggctatcc gcgcggtggc gctgggtgtg
    14941 gtggtgacgg cgttagtaca ggcagtgctt ggcggtatcg gcctcgccgt atccggcgta
    15001 ccttatgcaa cgttgctaac ggtgttaatg atcctctcct gccttgtcca gcttggcccg
    15061 ttgccggtac tgattccggc gattatctgg ctctactgga ctggcgatac cacctgggga
    15121 acggtattgt tagtgtggag cggtgtggtt ggcacgctgg ataacgtcat ccgcccaatg
    15181 ttaattcgca tgggtgccga tttaccgctg atcctgatcc tctctggtgt tattggtggt
    15241 ttgattgctt tcgggatgat cggtctgttt attggtccgg ttctgttagc cgtttcctgg
    15301 cgtctgtttg ccgcgtgggt ggaagaagtc ccgccgccga cggaccaacc ggaagaaatt
    15361 ctcgaagaac ttggcgaaat cgagaaaccg aataagtaat ttctcatcag gcggctctgc
    15421 cgcctgattg ttaacccccg ctaattgata tttgcttgct cttccccata ttttcctgct
    15481 taccgccatt cagctggtag tacctgtcgc aaattcttta cagtttttaa actaatgaga
    15541 cgaatctgat cgacgcaaaa agtccgtatg cctactatta gctcacggtt ataaatcaac
    15601 atattgattt ataagcatgg aaatcccctg agtgaaacaa cgaattgctg tgtgtagtct
    15661 ttgcccatct cccacgatgg gctttttttt aacatttttc cacttcgcta cctcgcccct
    15721 cactctttcc aataatgttg cacaagatac tgtgactctc ttcaagccgt cagcaggctg
    15781 ataatgttat ttatatttta ttgtttattt catgcagcca ttacacgatg aacgcttatg
    15841 aactccaggc actccgacat atttttgcca tgactattga tgaatgcgca cctggattgc
    15901 ccagaccggt gacagtgaaa gctggcgtca gtgggaaaat ggcaagtgtg ccattcctga
    15961 tcgtgtagtc gagcaactgt tggctatgcg tcagcaaaga aaaaaacatc ttcatgccat
    16021 catagagaaa ataaataacc gcatcggcaa caacaccatg cgttttttcc ccgacttaac
    16081 cgcatttcaa cgagtctacc ctgatggcaa ttttattgac tggaagatct atcaatcggt
    16141 agccgctgaa ggtaatgctg ttgaaaagca aattccccag ccagttggtg gacgccagcg
    16201 tagctaatga attaccgcaa atgaacagca aaccgttagt ctggctggaa ggtgtttcat
    16261 cggtactgtt cggtgtagcc gcattctcga ccttttatgt gattgtggtg tggatgccca
    16321 aatatgcgat ggcttttgct ggtatgtcag aagctgaggc attaaaaacc atctcttatt
    16381 acagtatggg ctcgttggtc tgtgtcttta tttttgccgc actactgaaa aaaatggtcc
    16441 ggcccatctg ggctaatgta tttaactctg cactggcaac aataacagca gccattatct
    16501 atctgtaccc ttctccactg gtgtgtaatg ccggcgcctt tgttatcggt ttctcagcag
    16561 ctggcggcat tttacagctc ggtgtttcgg tcatgtcaga gtttttccca aaaagcaaag
    16621 ccaaagtcac cagtatttat atgatgatgg gtggactggc taactttgtt attccgctga
    16681 ttaccggtta tctgtcgaac atcggccttc aatatatcat tgttctcgat tttactttcg
    16741 cgctgctggc cctgattacc gcaattattg tttttatccg ctattaccgc gtttttatta
    16801 ttcctgagaa tgatgtgcgg tttggcgagc gtaaatttag cacccggtta aacacaatta
    16861 agcatagagg ttaaaggagt taattatgga tgttaccgca aaatacgaat tgattgggct
    16921 gatggcctat cctatccgcc acagtttatc gcctgaaatg cagaataaag ccttagaaaa
    16981 agcgggattg ccatttacct atatggcctt tgaagtggat aacgatagtt ttcccggagc
    17041 aattgaagga ttaaaagctc taaaaatgcg cggaactggt gtatcaatgc cgaacaaaca
    17101 actggcttgt taatatgttg atgaattaac gccagcagcc aaactggtgg gtgccatcaa
    17161 caccatcgtt aatgatgatg gctacctgcg cggctataac accgacggca cgggccatat
    17221 tcgcgccatt aaagagagcg gttttgatat caaaggcaaa acaatggtgc tgttaggggc
    17281 cggaggtgcc tcaacggcta ttggcgcgca gggggcaatt gaaggtttaa aagaaattaa
    17341 actctttaac cgtcgggatg agttcttcga taaagccctc gccttcgcgc agcgggttaa
    17401 cgaaaacacc gattgtgtcg tcacggttac cgatctcgcc gatcagcaag cctttgctga
    17461 agccctggct tccgccgaca ttttaaccaa tggcacaaaa gtgggtatga aaccccttga
    17521 gaatgaatca ttagttaatg atatcagtct gttacatccg ggacttctgg tcactgaatg
    17581 cgtgtataac ccgcatatga cgaagttatt gcagcaggcg caacaagctg gttgcaaaac
    17641 gattgatgga tacggcatgt tgttgtggca aggggctgaa cagttcacgt tatggactgg
    17701 caaagatttc cctctggaat atgttaaaca ggtcatgggg ttcggtgcct gacaggctga
    17761 ccgcgtgcag aaagggtaaa aaaatgaaaa ccgtaactgt aaaagatctc gtcattggta
    17821 cgggcgcacc taaaatcatc gtctcgctga tggcgaaaga tatcgcccgc gtgaaatccg
    17881 aagctctcgc ctatcgtgaa gcggactttg atattctgga atggcgtgtg gaccactttg
    17941 ccgacctctc caatgtggag tctgtcatgg cggcggcaaa aattctccgc gaaaccatgc
    18001 cagaaaaacc gctgctgttt accttccgca gtgccaaaga aggcggcgag caggcgattt
    18061 ccaccgaggc ttatattgct ctcaatcgtg cagccatcga cagcggcctg gttgatatga
    18121 tagatctgga gttatttacc ggcgatgatc aggttaaaga aaccgtcgcc tgcgcccacg
    18181 cgcatgatgt gaaagttgtc atgtccaacc atgacttcca taaaacgccg gaagccgaag
    18241 aaatcattgc ccgtctgcgc aaaatgcagt ccttcgacgc cgatattcct aagattgcgc
    18301 tgatgccgca aagtaccagc gatgtgctga cgttgcttgc cgcgaccctg gagatgcagg
    18361 agcagtatgc cgatcgtcca atcatcacaa tgtcaatggc aaaaactggc gtaatttctc
    18421 gtctggctgg tgaagtattt ggctcggcgg caacttttgg tgcggtaaaa aaagcctctg
    18481 cgccagggca aatctcggta aatgatttac gcacagtatt aactatttta catcaggcat
    18541 aagcaataat atttcggcgg gaatatcctc ccccgccgaa ctaaaaaaaa tatattcaac
    18601 cgtatttaat aaaaatattt cgtgagtctc tgtgcgctaa atatccatct ggcgtaggga
    18661 aaatcacacc tgaatcagaa attaacaatg aaacctgtaa aaccacctcg tattaatgga
    18721 cgagtgccgg tcctgtcggc acaggaagcg gtgaattata ttcccgacga agcaacactt
    18781 tgtgtgttag gcgctggcgg cggtattctg gaagccacga cgttaattac tgctcttgct
    18841 gataaatata aacagactca aacgccacgt aatttatcga ttattagtcc aacagggctt
    18901 ggcgatcgcg ccgaccgtgg tattagtccg ctggcgcaag aaggtctggt gaaatgggca
    18961 ttatgtggtc actggggaca atcgccgcgt atttctgaac tcgcagaaca aaataaaatt
    19021 attgcttata actatccaca aggtgtactt acacaaacct tacgcgccgc cgcagcccac
    19081 cagcctggta ttattagtga tattggcatc gggacatttg tcgatccacg ccagcaaggc
    19141 ggcaaactga atgaagtcac gaaagaagac ctgattaaac tggtcgagtt tgataacaaa
    19201 gaatatctct attacaaagc gattgcgcca gatatcgcct tcattcgcgc taccacctgc
    19261 gacagtgaag gctacgccac ttttgaagat gaggtgatgt atctcgacgc attggttatt
    19321 gcccaggcgg tgcacaataa cggcggtatt gtgatgatgc aggtgcagaa aatggttaag
    19381 aaagccacgc tgcatcctaa atctgtccgt attccgggtt atctggtgga tattgtggtg
    19441 gtcgatccgg atcaaagcca actgtatggc ggtgcgccgg ttaaccgctt tatttctggt
    19501 gacttcaccc ttgatgacag caccaaactt agcctgcccc taaaccaacg taaattagtt
    19561 gcgaggcgtg cattattcga aatgcgtaaa ggcgcggtgg ggaatgtcgg cgtcggtatt
    19621 gctgacggta ttggcctggt cgcccgggaa gaaggttgtg ctgatgactt tattctgacg
    19681 gtagaaacag gtccgattgg cggaattact tcacagggga tcgcctttgg cgcgaacgtg
    19741 aatacccgtg ccattctgga tatgacgtcc cagtttaatt tttatcacgg tggtggtctg
    19801 gatgtttgtt atttgagttt tgctgaagtc gaccagcacg gtaacgtcgg cgtgcataaa
    19861 ttcaatggca aaatcatggg taccggtgga tttattgata tcagtgccac ctcgaagaaa
    19921 attattttct gcggcacgtt aaccgcgggc agtttaaaaa cagaaattgc cgatggcaaa
    19981 ttaaatatcg tccaggaagg acgggtgaag aaatttattc aggaactacc ggaaattact
    20041 tttagcggaa aaatcgcgct cgaacgggaa ctggatgttc gttacatcac cgaacgtgct
    20101 gtattcacgc tgaaagaaga cggcctgcat ttaatcgaaa tcgcccctgg cgtcgattta
    20161 caaaaagata ttctcgacaa aatggatttc accccagtga tttcgccaga actcaaactg
    20221 atggacgaaa gattatttat cgatgcggcg atgggttttg tcctgcctga agcggctcat
    20281 taacaggagt atcacgatgg atttttcttt aactgaagaa caagaactgc tgctggccag
    20341 tattcgcgaa ctgattacga ctaactttgc ggaagagtat ttccgcacct gcgatcaaaa
    20401 cgggacatat ccgcgtgagt ttatgcgggc gctggcggat aacggcattt ctatgcttgg
    20461 cgtgccggaa gagttcggtg gcatccctgc ggattacgtc acccagatgc tggcgctgat
    20521 ggaagtgtca aaatgcggtg ctccggcatt tttgattacc aacggtcaat gtattcacag
    20581 tatgcgccgt ttcggttctg cagagcagct acgtaaaacg gcagagagca ccctggaaac
    20641 gggtgatccc gcctatgccc tggcgttgac ggagccaggc gcaggctcag acaacaacag
    20701 tgccactacc acttacacgc gtaaaaacgg caaggtttac atcaacggac agaaaacctt
    20761 tattaccggt gcgaaagagt acccgtatat gctggtgttg gcgcgcgatc cgcaaccgaa
    20821 agatcccaaa aaagccttca ccctgtggtg ggtcgactcc agtaagcccg gcattaagat
    20881 taacccgctg cataaaatcg gctggcatat gctcagcacc tgcgaagtct atctcgacaa
    20941 cgtggaggtt gaagagagcg acatggtggg cgaagaagga atgggtttcc tcaatgtgat
    21001 gtacaacttt gagatggagc gcctgatcaa cgccgcgcgc agcaccggct ttgccgaatg
    21061 cgcctttgaa gatgccgccc gctatgccaa ccaacgtatc gcttttggta agcccattgg
    21121 tcataaccag atgatccagg aaaaactggc gctgatggcg attaagatcg acaacatgcg
    21181 caacatggtg ctgaaagtgg catggcaagc cgatcagcat cagtcactgc gcaccagcgc
    21241 ggcgctggca aaactgtatt gcgcacgtac cgcaatggaa gtcattgatg atgcgattca
    21301 aatcatgggc ggtctgggct ataccgatga agcgcgcgtc tcccgcttct ggcgtgatgt
    21361 ccgttgtgaa cgtatcggcg gcggtacaga cgaaattatg atttacgtag caggtcggca
    21421 gatcctgaaa gactatcaga acaaataatc tgcaggcggc gcagcttctt aacaaactgc
    21481 gccgccagat ttatccaaca agacttaccg gttgaggaaa ttcagatagg atttcttgtt
    21541 ccgcaaaaca gtttttaaat tgtctacggt aatcgctggg cgaacaaccg acatggcgca
    21601 aaaacagttt ggcaaagtga tcgacatttt cataacccac acgccaggag atctctgcct
    21661 gtgataattc agtattagtg agtgaccatt ttgcttccgt catacgccgt tgaataacat
    21721 agttaatagg ggaaatacga tactctttgg taaattcatg gcaaatataa ctgacgctgg
    21781 cacgaaattt tttcgataac tgttctaaag tgattttttc gcgataatta ttactcagat
    21841 agaaaagaat atcttttatc agaacatctt ttttaatata cccctgctcc gaacgataag
    21901 catttttaaa attttcgtag taaagaatgg ctaacgtata ggcaaatgca tcccataacg
    21961 aagatgtttg gctattttta ctttgcggca aaatcacact tagctcatta aataaggttt
    22021 ttatgacttc ttttccctgc cccgcggcaa ttaccggaca agaatgcggt tgcagtagct
    22081 gattttcctc agccccctga aactgaaagc cgtacagcgc acaggtacac gtcgttgccg
    22141 gatcgttaac gtcagaggcc accgcatgca gcctgccgcg ttctatcacc acaatgtcat
    22201 ccgcatgtgc gacatataac gaagaatcga tggtaaacct tgcaacccct ttcttgacgt
    22261 aaatcaactc ggtttcattg tcatgaacgt gatgtccgga ctcccatttt ggatcatcgc
    22321 taaatgcaaa acgtgaaagc cgtggcgttt taccggcgac aaacagcgtt tcactggcgt
    22381 tatcaaaaca gcgttgatac atgacaacct ccctattcca tgagcaagca aaaacaatat
    22441 atgccggata aaatacggcg tgtattgcgg ttatacaacc gcgtttagcg agagctaaac
    22501 aagattattt acactgaatg caagattgta cggtcatgga ataactatat tgccgcaaat
    22561 tgcacttttg ttttagcgat cgcatttttt tgcaagattg ttggcaagga aaacagcttg
    22621 ctccgtcgaa aacccccacc gctatcgcac actattttca ggccattttt accttccatc
    22681 ggagatggtt ccgtatgcga ctcacaggag aaatcatgaa aataataacc tgctttaagc
    22741 tggtgcctga agaacaggac attgttgtca ctccagaata caccctgaat ttcgacaatg
    22801 ccgacgccaa aatcagccag ttcgatctca atgccattga agctgcaagc cagctcgcaa
    22861 cagatgacga tgagatagcc gcgctgaccg ttggcggctc attgttgcag aactcgaaag
    22921 tgcgcagtgc gcaaagatgt gctatcccgc gggccgcaca gcctgtattt ggtgcaggat
    22981 gcgcaacttg aacatgcact gcctctcgat accgcaaaag cgctggcggc agcaattgaa
    23041 aagatcggct tcgatttact gatctttggt gaaggttccg gcgaccttta tgcccagcag
    23101 gttggcttgc tggtcggaga aattctgcaa cttccggtga ttaatgcagt gagtgctatt
    23161 cagcgtcagg gtaatacact ggtgattgaa cgcacgcttg aagatgatgt tgaagttatt
    23221 gaactctctg ttccagccgt gctctgcgtc acctccgata ttaacgtgcc acgcattcct
    23281 tcgatgaaag ccattctcgg cgcgggtaaa aaaccggtaa atcagtggca ggcaagtgat
    23341 attgactgga gccagagcgc gccacttgct gaactggtcg gcattcgcgt accgccgcaa
    23401 acagaacgta agcacatcat tatcgataac gattcgccgg aggccattgc cgagctggcg
    23461 gaacatctga agaaagccct gaactgaagc ctacggagaa gaaacgatga gtcaattaaa
    23521 cagcgtctgg gtctttagcg ataatcctga acgttatgct gaactgtttg gcggcgctca
    23581 gcaatggggc caacaggtgt atgccattgt acaaaatacc gaccaggcgc aggcagttat
    23641 gccttatggt ccaaaatgta tttatgttct tgagcaaaac gacgcgctgc aacgcactga
    23701 aaattacgcc gaaagcattg ctgccccgct gaaagataaa caccccgcta tgttgctgtt
    23761 ggtcgcgacg aaacgtggta aagctctggc agcacggtta agtgtgcaac tgaatgcggc
    23821 gctggtgaac gatgccacgg cggtggatat tgtcgatggt cacatttgcg ccgaacaccg
    23881 gatgtatggc gggttagcgt tcgctcagga aaagatcaac agcccgctgg cgatcattac
    23941 ccttgcaccc ggtgttcagg aaccgtgcac cagtgatacc tctcatcagt gcccgacaga
    24001 aacggtacct tatgttgctc cgcgtcatga aattctctgt cgcgaacgcc gtgcgaaagc
    24061 cgcaagcagc gtggacctga gcaaagcaaa acgtgtggtt ggcgtcggtc gtggtctggc
    24121 ggcgcaggat gacctgaaaa tggtccacga actggcggcg gtgctgaatg ctgaagtcgg
    24181 ctgttcacgt ccaattgccg aaggcgagaa ctggatggag cgtgaacgtt atatcggtgt
    24241 ctccggcgtg ttgctgaaat ccgatctcta cctgacgctg gggatctccg ggcagatcca
    24301 gcatatggtt ggcggcaacg gcgcaaaagt gattgtcgcc atcaataaag ataaaaatgc
    24361 gccaatcttc aattatgccg actacggtct ggtgggcgat atctacaaag tcgtccctgc
    24421 cctgattagc cagttgagcc gctaattccc ctttcaacca catctcccgc tgtaaacgca
    24481 gcgggaaggg agcataacgc atgtcggatg acaaatttga tgccattgtg gtcggtgcgg
    24541 acgttgctag tagcgttgcc gcactggtca tggcgcgagc cggactggat gtcctggtga
    24601 tagaacgcgg cgacagtgcc ggatgtaaaa acatgaccgg cgggcgtctt tatgcccaca
    24661 cacttgaagc aatcattcca ggctttgcag tatcagcgcc ggtagaacgc aaggtcacac
    24721 gcgagaaaat ctccttctta accgaagaaa gcgccgttac cctcgatttt caccgcgagc
    24781 aaccagatgt tccgcaacac gcatcttata ccgtattgcg taatcgtctg gacccgtggt
    24841 tgatggaaca agccgagcag gcgggcgcac agtttatccc gggcgttcgc gtcgatgcgt
    24901 tggttcgtga aggaaacaag gtcactggcg tgcaggccgg ggatgatatt ctccaagcga
    24961 atgtggtggt tctagctgat ggcgtaaact cgatgcttgg ccgctcgctg ggaatggttc
    25021 ccgcttccga tccgcatcat tacgctgttg gtgttaaaga ggttattggc ctcacaccag
    25081 aacagatcaa cgatcgcttt aatattacgg gcgaggaagg tgccgcctgg ctgtttgccg
    25141 gctccccttc tgacggcctg atgggcgggg gatttctcta taccaacaag gattccatat
    25201 ccttggggct ggtttgtgga ttgggtgata tcgcccatgc gcaaaaaagc gtgccgcaaa
    25261 tgctggaaga ttttaaacaa caccccgcca ttcgcccact aattagcggc ggcaaactgc
    25321 ttgaatattc cgcgcatatg gtgccagaag gcggtctggc aatggtgccg cagatggtta
    25381 acgatggcgt gatgatcgtt ggtgacgccg caggcttctg cctgaatttg ggttttacag
    25441 ttcgtggcat ggatttagcc attgcatcgg ctcaggctgc cgccacaacg gtgatcgccg
    25501 ccaaagaacg cgaggatttc tccgccagca gtctggcgca atacaaacgt gagctggaac
    25561 aaagctgcgt catgcgcgat atgcagcatt tccgcaagat cccggcgctg atggaaaacc
    25621 cgcgcctgtt tagccaatac tcacgaatgg tcgccgacat catgaacgag atgttcacca
    25681 ttgacggcaa accaaaccag ccggtacgca aaatgatcat gggacacgcg aagaaaattg
    25741 ggctgatcaa cttgctgaaa gatggcatta agggagcaac cgcgctatga gccagaacgc
    25801 tacggttaac gtcgacatca aattaggcgt caataaattc catgttgatg agggccaccc
    25861 gcatatcatt ctggcggcaa atcccgatat caatgaattc cgtaaattaa tgaaagcttg
    25921 ccctgccgga ctttataagc aggatgacgc cggcaacatt catttcgatt ccgccggttg
    25981 tctggagtgc ggcacctgtc gggtgctgtg cggcaacact attctcgaac agtggcaata
    26041 tcccgcaggc accttcggta ttgattttcg ctacggctaa tcatgcatcc cacaggcccg
    26101 catctcgggc ctgatgttct gtttcgagag tccaaaatga aagtgacatt aacgttcaac
    26161 gaacaacgtc gtgcggcgta tcgtcagcaa gggttatggg gcgatgcttc actggccgat
    26221 tactggcagc agaccgctcg tgcgatgcca gacaaaattg ccgtggtcga caatcatggt
    26281 gcaacgtaca cctatagcgc gctcgatcac gccgcgagct gtctggcaaa ctggatgtta
    26341 gcgaagggta ttgaatcagg cgatcgcatc gcatttcaac tgcctggctg gtgtgaattt
    26401 accgttatct atcttgcctg cctgaaaatc ggtgcggttt ccgtgccgct gttgccttcc
    26461 tggcgggaag cagaactggt gtcggtgctc aataagtgtc aggcaaaaat gttctttgca
    26521 ccgacgttgt ttaaacaaac acgtccggta gatttaatcc tgccgctgca aaatcagctt
    26581 ccacaactac aacaaattgt cggcgtggac aaactggctc ccgccacctc ttccctctca
    26641 ttaagtccga ttatcgccga caatacctca ctgaccacgg cgataacgac ccacggcgat
    26701 gaattagctg cggtgctgtt tacctccgga accgagggtc tgccaaaggg cgtgatgcta
    26761 acgcataaca atattctcgc cagtgagcgg gcttattgcg cgcgactgaa tctgacctgg
    26821 caggatgtct ttatgatgcc tgcgccactt ggtcacgcaa cgggctttct gcatggcgta
    26881 acggcaccat tcttaattgg cgctcgcagc gtgttgttag atattttcac tcctgatgcg
    26941 tgtctcgcgc tgcttgagca gcagcgttgc acctgtatgc taggcgcaac gccgtttgtc
    27001 tatgatcttt tgaatgtact agagaaacaa cccgcggacc tttcagcgct gcgtttcttt
    27061 ctttgcggcg gtaccacaat ccccaaaaaa gtggcacgtg aatgccagca gcgcggcatt
    27121 aaattattaa gtgtttatgg ttccacagaa agttcgccgc atgcggtggt gaatctcgat
    27181 gatcctttgt cgcgctttat gcacaccgat ggttacgctg ccgcaggtgt agagattaaa
    27241 gtggtcgatg acgcacgcaa gaccttaccg ccaggttgcg aaggtgaaga agcctcgcgt
    27301 ggccccaatg tgtttatggg gtattttgat gaacctgaat taaccgcccg tgcgctggac
    27361 gaagaaggct ggtattgcag cggcgatctc tgccgtatgg atgaggctgg ctatataaaa
    27421 attaccggac gcaaaaaaga tattattgtc cgcggcggcg aaaatattag cagccgtgaa
    27481 gtggaagata ttttattgca gcatcctaaa attcacgatg cctgtgtggt tgcaatgccc
    27541 gatgaacgtt taggtgaacg atcatgcgct tatgtcgtgc tgaaagcgcc gcatcattca
    27601 ttatcgctgg a
//
LOCUS       DAJVOY010000037        27527 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVOY010000037 DAJVOY010000000
VERSION     DAJVOY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.037"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(174..704)
                     /gene="ppa"
                     /locus_tag="OQL90_001402"
     CDS             complement(174..704)
                     /gene="ppa"
                     /locus_tag="OQL90_001402"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1368227.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1014..1970
                     /gene="ytfQ"
                     /locus_tag="OQL90_001403"
     CDS             1014..1970
                     /gene="ytfQ"
                     /locus_tag="OQL90_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCS1368228.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2110..3612
                     /locus_tag="OQL90_001404"
                     /pseudo
     CDS             2110..3612
                     /locus_tag="OQL90_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            3626..4648
                     /locus_tag="OQL90_001405"
     CDS             3626..4648
                     /locus_tag="OQL90_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1368229.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4635..5630
                     /gene="yjfF"
                     /locus_tag="OQL90_001406"
     CDS             4635..5630
                     /gene="yjfF"
                     /locus_tag="OQL90_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS1368230.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OQL90_001407"
     CDS             complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OQL90_001407"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1368231.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6837..8210
                     /gene="mpl"
                     /locus_tag="OQL90_001408"
     CDS             6837..8210
                     /gene="mpl"
                     /locus_tag="OQL90_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1368232.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8293..8844)
                     /locus_tag="OQL90_001409"
     CDS             complement(8293..8844)
                     /locus_tag="OQL90_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1368233.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8938..10290
                     /gene="pmbA"
                     /locus_tag="OQL90_001410"
     CDS             8938..10290
                     /gene="pmbA"
                     /locus_tag="OQL90_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1368234.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10474..10860
                     /gene="cybC"
                     /locus_tag="OQL90_001411"
     CDS             10474..10860
                     /gene="cybC"
                     /locus_tag="OQL90_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS1368235.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OQL90_001412"
     CDS             complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OQL90_001412"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1368236.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OQL90_001413"
     CDS             complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OQL90_001413"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1368237.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OQL90_001414"
     CDS             complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OQL90_001414"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1368238.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OQL90_001415"
     CDS             complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OQL90_001415"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1368239.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OQL90_001416"
     CDS             complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OQL90_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1368240.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18377..18430
                     /gene="mgtL"
                     /locus_tag="OQL90_001417"
     CDS             18377..18430
                     /gene="mgtL"
                     /locus_tag="OQL90_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1368241.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18571..21267
                     /gene="mgtA"
                     /locus_tag="OQL90_001418"
     CDS             18571..21267
                     /gene="mgtA"
                     /locus_tag="OQL90_001418"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1368242.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OQL90_001419"
     CDS             complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OQL90_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1368243.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYETFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OQL90_001420"
     CDS             complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OQL90_001420"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1368244.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OQL90_001421"
     CDS             complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OQL90_001421"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1368245.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OQL90_001422"
     CDS             complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OQL90_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1368246.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23759..24154)
                     /locus_tag="OQL90_001423"
     CDS             complement(23759..24154)
                     /locus_tag="OQL90_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1368247.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OQL90_001424"
     CDS             complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OQL90_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1368248.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            25069..25662
                     /locus_tag="OQL90_001425"
     CDS             25069..25662
                     /locus_tag="OQL90_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1368249.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25807..26259
                     /gene="tabA"
                     /locus_tag="OQL90_001426"
     CDS             25807..26259
                     /gene="tabA"
                     /locus_tag="OQL90_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1368250.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26382..27527
                     /locus_tag="OQL90_001427"
                     /pseudo
     CDS             26382..27527
                     /locus_tag="OQL90_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         6604 a         6815 c         7277 g         6831 t
ORIGIN      
        1 tccagcaagt ggctatgaac agcaaaatgg tggtcgggtt tgcttgttct taccgttcaa
       61 gccttgcaac cgctgagaat aatgatggtg gaatagagag tttatttatc gcgggcataa
      121 aaaaaccctt actaaccgaa gcccggcgtt cagggttatt gcgctagaag aacttattta
      181 ttctttgcgc gctcgaagga agcaacgatt tcagctttag cggcttctgc gttttcccaa
      241 ccttcaactt tcacccactt gcctttttcg aggtctttgt agtgctcgaa gaagtgagcg
      301 atttgcgctt tcagcagttc aggcagatcg tttacgtctt taatgtgatc gtattctttg
      361 ctcagcttgc tgtgcggaac cgcaaccagt ttcgcatctt caccggcttc gtcggtcatt
      421 ttcagaacgc caaccggacg gcaacggatc acagaaccag gctgcagcgg gtacggagtc
      481 gggaccagta cgtcaaccgg gtcaccgtcc agagacaggg tgtggttgat gtaaccgtag
      541 ttgcacggat agaacatcgc ggtggacatg aagcggtcaa cgaacagtgc gccgctctct
      601 ttgtcgattt cgtatttgat cggatctgcg ttagccggga tctcaataac aacgtagatg
      661 tcttccggca gatctttacc cgcagggacg ttgagtaagc tcatgtctgt ttcctttaaa
      721 aatatgtggc aaacaagtgc cgagtattat agccaactcg cgccgaatgt ctttgcttgt
      781 tttcgtcttc attttccttt ttcgccagtt ttaagacggt atacagagca ggaaaatcca
      841 taaccgtagc cgcatttttc atagtgagat gaaagcgatt acaaacttgt gattaacgtt
      901 ttatttactt ttttgaagtg tgatgtaacg caatccgtta cataacgaat tgtctatagt
      961 tttttcgcga acatcttttc accaataata attaccccga ctaggacaac cctatgtgga
     1021 aacgcttact tgtagtctct gcagtctcgg cagccatgtc gtctatggcg ttggccgctc
     1081 cattaaccgt aggattttcg caggtcggat cggaatccgg ctggcgcgcc gcagaaacca
     1141 atgtggcgaa aagtgaggcc gaaaaacgcg gaattacgtt gaaaattgcc gatggtcagc
     1201 aaaagcagga aaaccggatt aaagcggtac gttccttcgt tgcacaaggg gtggatgcga
     1261 tctttatcgc tccagtggtt gcgacgggtt gggaaccggt attaaaagag gcgaaagatg
     1321 ccgaaatccc ggtcttcttg ctcgaccgtt ccatcgatgt gaaagacaaa tctctctata
     1381 tgaccaccgt tactgccgac aacatcctcg aaggtaagtt gatcggtgac tggctggtaa
     1441 aagaagtgaa tggcaaacca tgcaacgtgg tggagctgca gggcaccgtt ggggccagcg
     1501 tcgccattga ccgtaagaaa ggctttgccg aagccattaa gaatgcgcca aatatcaaaa
     1561 ttatccgctc gcagtcaggt gacttcaccc gcagtaaagg caaagaagtc atggagagct
     1621 ttatcaaagc ggaaaacaac ggcaaaaaca tctgcatggt ttacgcccat aacgacgaca
     1681 tggtgattgg tgcaattcag gcaattaaag aagcgggcct gaaaccgggc aaagatattc
     1741 tgacaggttc tatcgatggc gtaccggata tctacaaagc gatgattgat ggcgaagcga
     1801 acgccagtgt tgaactgacg ccgaatatgg cgggacccgc ttttgacgcg ctggagaaat
     1861 acaaaaaaga cggcaccatg cctgaaaagc tgacgttaac caaatccacc ctttacctgc
     1921 ctgataccgc aaaagaagag ttagagaaga agaaaaatat ggggtattga gggttgctat
     1981 gcctgatgcc gattcgtagg ccggataagg cgctcgcgcc gcatccggcg atggtgcact
     2041 gaagcctgat gcgacgctta ccgcgtctta tcatgcctac tgggagcacg ctttacaccg
     2101 ggggaaacca tgacgaccga ccaacaccag gaaatcctcc gcaccgaagg attaagtaaa
     2161 tttttccccg gcgtcaaagc gttagacaac gttgatttca gcctgcgccg tggcgaaatc
     2221 atggcgctgc tcggtgaaaa cggggcggga aaatcaacgc taatcaaagc attaactggt
     2281 gtataccacg ccgatcgcgg caccatctgg ctggaaggcc aggttatctc accgaaaaat
     2341 accgcccacg cgcaacaact cggcattggc accgtctatc aggaagtcaa cctgctaccc
     2401 aatatgtcgg tcgctgataa tctatttata ggccgcgaac ccaaacgctt cggccttcta
     2461 cgccgcaaag agatggaaaa gcgcgccacc gaactgatgg catcttacgg tttctccctc
     2521 gacgtgcgcg aaccgctcaa ccgcttttca gtcgcgatgc agcaaatcgt cgccatttgc
     2581 cgggctatcg atctctccgc caaagtgctg atcctcgatg aacccaccgc cagcctcgac
     2641 acctaggaag tggagctatt atttggcctg atgcgtcagt tgcgcgatcg cggcgtcagc
     2701 ctgatctttg tcactcactt tctcgatcag agctatcagg tcagcgatcg gatcaccgtt
     2761 ttacgcaacg gcagtttcgt aggctgtcgg gaaacgcgcg agctaccgca gatcgaactg
     2821 gtaaaaatga tgctggggcg cgagctggat acccacgcgc tacagcgtgc cgggcgaaca
     2881 ttgttgagcg acaaacccgt tgccgcgttc aaaaattacg gcaaaaaagg aacgatagca
     2941 ccgtttgatc tcgaagtgcg ccccggcgag atcgtcggcc tggcaggctt gctgggatcc
     3001 ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac ctgctgacag cggcacggcg
     3061 ttgatcaaag gcaaaccgca aaacctgcga tcgccacatc aggcttcggt actgggcatc
     3121 ggcttctgcc cggaagacag gaaaaccgat ggcatcatcg ctgccgcctc ggtgcgggaa
     3181 aatatcatcc tcgctctcca ggcccagcgc ggctggctac gacccatttc ccgcaaagag
     3241 cagcaagaga ttgccgaacg ctttatccgc cagcttggca ttcgcacacc ttcaactgaa
     3301 caaccgattg aatttctctc cggcggcaat cagcaaaaag tgttgctttc acgttggcta
     3361 ctgacccgac cgcaatttct gatcctcgat gagccaaccc gtggcattga tgttggtgcc
     3421 cacgccgaga tcatccgcct gattgaaacg ctatgcgccg atggtctggc gctgctggtg
     3481 atctcctccg aactggaaga actggtgggc tatgccgacc gggtgatcat catgcgcgat
     3541 cgcaaacagg tggcggagat ccggctggca gagctttccg ttccggctat catgaacgcc
     3601 attgcggcgt aaggagaaca gtgtgatgcc tcaatctctt ccggacacca ctacgccaaa
     3661 aaggcgcttt cgctggccga cggggatgcc gcagctggta gcactattgc tggtgctgct
     3721 ggtcgacagc ctggtagccc cgcatttctg gcaggtagtg cttcaggatg ggcgtttgtt
     3781 cggtagcccc atagacattc ttaaccgtgc agcccccgtt gcgttactgg cgattggtat
     3841 gacgctggtg atcgccaccg gtgggattga tctctccgta ggggcggtga tggctatcgc
     3901 cggagccaca acggctgcga tgacggtcgc ggaagtcagc ctgccgattg ttttgttaag
     3961 cgccctaggc accggcatcc tggcgggatt gtggaacggc atactggtag cgatcctcaa
     4021 aattcagcca tttgtcgcca ccctgatcct gatggtcgcc gggcgcggcg tggcgcaact
     4081 gatcacctcc ggacagatcg tcacgtttaa ctcgccggat ctctcatggt ttggcagtgg
     4141 atcgctgttg ttcctgccaa cgccagtcat tattgcggta ctgacgcttc tcctgttctg
     4201 gctgttgacc cgcaaaacgg cactggggat gtttatcgaa gccgttggta tcaacattcg
     4261 ggcggcaaaa aatgccgggg taaacacgcg gatcatcgtc atgctcactt atgtgttgag
     4321 cgggctgtgt gcggcgattg cgggcattat cgtggcggcg gatattcgcg gtgccgatgc
     4381 caacaacgcc ggattatggc tggagctgga cgccattctc gcggtggtga ttggcggcgg
     4441 atcgctgatg ggcgggcgct ttaacctgct gctctcggta gtgggggcgc tgattattca
     4501 ggggatgaac accggaattt tgctttcggg ctttccaccg gagatgaacc aggtggtgaa
     4561 agcggtggtg gtgctttgcg tgctgattgt ccagtcgcaa cgctttatca gtctgattaa
     4621 aggagtacgt agccgtgata aaacgtaatt tgccactgat gatcaccatc ggcgtctttg
     4681 tgctgggcta tttctactgc ctgacgcagt ttcccggttt tgcttccaca agagtgatct
     4741 gcaatatcct gaccgataac gcttttctgg ggattattgc cgttggtatg acctttgtga
     4801 tcctctctgg cggtatcgat ctctccgtcg gttcggtgat cgcctttact ggcgtgtttc
     4861 tggcaaaagt ggttggcgat ttcggcctct cgccgctgct ggcgtttccg ctagtgctgg
     4921 tgatgggctg tgccttcggc gcatttatgg ggcttctgat cgacgccctg aagatcccgg
     4981 catttatcat tacgcttgcg gggatgttct ttttgcgcgg cgtcagctat ctcgtttcgg
     5041 aagagtcgat tccgataaac catcccattt atgacacgct ctcaagcctt gcgtggaaaa
     5101 tccccggcgg cggtcgcctt agtgcgatgg ggctgttgat gctggcggtg gtggttatcg
     5161 gtattttcct tgcgcatcgt acccgttttg gcaatcaggt gtacgctatt ggcggcaacg
     5221 cgacgtcggc gaacctgatg gggatttcta ctcgcagcac tactattcgc atttatatgc
     5281 tctccaccgg actggcaaca ctggcgggga ttgtcttctc gatttatacc caggccggat
     5341 atgcactggc gggcgtaggt gtggaactgg acgccatcgc gtcggtggtg attggcggta
     5401 cgttgttaag tggtggcgtc gggacggtgc ttgggacgct ttttggcgtg gcaattcagg
     5461 gactgatcca gacttacatc aactttgacg gcacgctgag ttcctggtgg acgaaaatcg
     5521 ccatcggtat tttgttgttt atttttatag cattacagcg tgggttaacg gtgctgtggg
     5581 agaatcgtca gagttcgcca gtgacaagag tcaacattgc gcagcgataa aaatgcctct
     5641 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
     5701 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
     5761 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
     5821 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
     5881 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
     5941 cgatataacg tgaggtatac gggcggttgg tggatttatc ttcttcctgg cagaatttaa
     6001 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
     6061 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
     6121 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
     6181 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
     6241 ccggcgtgcc gacgggcgta actcggcggt agatggagaa aatggtaccg acagagacgt
     6301 taacatcgat gttggacgag ccatccagtg ggtccatcag caccacgtat tttgcatgtt
     6361 cacagccttc aaagacaaca atctcatctt cttcttcaga ggcaatgcct gcaacgatat
     6421 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
     6481 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
     6541 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
     6601 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     6661 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     6721 tcaatgttgc gctcaggtga atcgcgccag caaattacgg attatcctga aatgcgtttc
     6781 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     6841 gcattcatat tttaggaatt tgtggcacgt ttatgggcgg tctggcgatg ctggcgcgcc
     6901 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     6961 ttgagaagca aggcattgaa ctgattcagg gttacgatgc cagccagctc gatccgcagc
     7021 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     7081 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     7141 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     7201 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     7261 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     7321 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     7381 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     7441 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     7501 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     7561 gtgagcaggg gcactggcag gcgaaaaagc tgaccactga tgcttccgaa tgggaagtct
     7621 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     7681 ataatggcct gatggcgatt gcggcggccc gccatgttgg cgtagcgccg gcagataccg
     7741 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     7801 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     7861 cggcgctgcg tggcaaagtt ggtggtacag cgcgcattat tgctgtgctg gaaccgcgct
     7921 cgaataccat gaaaatgggg atctgcaaag acgatttggc accttcatta ggtcgtgccg
     7981 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     8041 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     8101 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     8161 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcctca
     8221 gcctgagata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     8281 caacaacgaa aattactctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     8341 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagagtacg
     8401 cagttgctga cgatcggcat cgggccacag atttaatacc tcagcgatgg cgtcatcacc
     8461 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     8521 gtgacggttt ttcagcttat ccagcgcctg acggatcggt tctacatcgc gctggcgcag
     8581 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     8641 ctcaatagcc gcgcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     8701 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     8761 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     8821 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     8881 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     8941 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     9001 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     9061 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     9121 agcgatggcg cgctggggat cactgtttat caccagaacc gcaaaggtag cgcatcatcc
     9181 accgatttaa gcccgcaggc cattgcccgt actgtacagg cggcgctgga tattgcccgt
     9241 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     9301 ccagatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     9361 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     9421 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     9481 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     9541 gagcgtgatt acgcctacac cattggtcgt gcgatgagcg atctgcaaac gccagagtgg
     9601 gttggggtcg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     9661 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     9721 ggggcgatag cgggtggatc ggtttaccgt aaatctacct tcctgctgga ttcgctgggt
     9781 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     9841 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     9901 ggcatcctga ctcagtggct gctgaccagc tattcggcgc gaaaactggg gctgaaaagc
     9961 accggacatg cgggcggtat tcacaactgg cggattgccg gacaaggtct aagcttcgag
    10021 cagatgctca aagagatggg caccgggctg gtggtgacgg aattgatggg ccagggcgtg
    10081 agtgcaatta ccggtgatta ttcccgtggt gcggcgggct tctgggtaga aaacggcgaa
    10141 attcagtatc cggttagcga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
    10201 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
    10261 ttgccggaga tgaaaatcgc cggacagtaa aaaaactggc gcgacctgtc gcgctataaa
    10321 ctaaaaaatt acacaaaatc attcgcactg catcgagacg gaaagtgaat gagggccggg
    10381 gagcgtactc gcagtacgtg acccggtcga atgagcgtag tcgtcgatga ggcagtgtga
    10441 atgatgaagt gtaaataaaa aaggaagtga gcaatgcgaa aaagcctgtt agctattctt
    10501 gcagtctcct cgttggtatt cagttcggcg tcgtttgccg ctgatcttga agacaatatg
    10561 gaaaccctca acgacaattt aaaagtggtc gaaaaagccg ataacgcggc gcaagtcaaa
    10621 gacgcgttaa cgaagatgcg cgccgcagcg ctggatgcgc aaaaagcaac gccgccgaag
    10681 ctcgaaggca aatcaccgga cagcccggag atgaaagact tccgtcacgg tttcgatatt
    10741 ctggtcggtc agattgacga cgcgctgaag ctggcaaatg aaggtaaagt aaaagaagcg
    10801 caggctgccg cagagcaact gaaaacgacc cgcaacgcct atcaccagaa gtatcgttaa
    10861 ttcctcattt ccctgttgcc tgcactcagg taacagggaa cttatcatcg caaatgatgc
    10921 accacctgat tgctgctgcc gcgccagatc agggaagggt cttttaaatc ctgcacaaat
    10981 ttgccgtcga ccagcacgtt aatcaaatca acaacctgca tttgcgctgc gttgagttcg
    11041 tcgagtttat agcctgtcca cacccagatg tctttacccg gacactcggc gcggatgcgt
    11101 tgtaccagtt tcagaatatc cggcacgttt tgcggatgca gcggatcgcc gccggagagg
    11161 gaaataccct ggcgtttgat acgagtgtca ttcagatcgt taatgatctg gtcttccatt
    11221 gctttagtaa atggctgacc ggaatttacc cgccatgtgc ttttgttata gcaaccgggg
    11281 cattcatgaa cacacccgga gacaaacaga gtgcaacgag tgccggggcc gttgacgatg
    11341 tcgacaggat agtactgatg ataattcatt atttcgcctg gcttatcagt ccgcgtgtag
    11401 actgcatccg gtaatcaatg cctgatgcga cgctttcaca tcacatccgg catttgctta
    11461 gtgcagatca acctatctgc ccatttccca aatgtttaac gcggcgctta acttcttcct
    11521 gcttgccggc gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag
    11581 ttaccgacac acgggaggcg tcatggttac cacatttcgg gcaggtgaag cctttgctgg
    11641 tgcactcgaa ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc
    11701 cgtaatacgg tacatgctga tagctgtaat cccagacatc ttccagcgcc ttcaggttgt
    11761 gctgaatgtt tgaatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag
    11821 gcgcttcaaa gtcgatcttg tcgtacgggt tcaccttctt ctccacatcg aggtggaaac
    11881 tgttggtgta gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga
    11941 gacggcagaa gcggtcgcac aggttttcac tcggcgtgct gtagagactg aaaccataac
    12001 ccgtttcttc cttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt
    12061 tcgcgcgaag ctgctcgttg tcgtaaacat gctcgccgcc gaacagcgcg ttaatggttt
    12121 cgtggatgcc gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa
    12181 catcatcgtc agcattgaga cgcacgccac aagcaccttc catatagagg atcggggcca
    12241 cgcgcgcttt cacgccttcg agacgagcga tacgggtcat cagcgcctta cgtgccagca
    12301 ccagacgttc atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa
    12361 tacgcggcag gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc
    12421 cgttttcatt ttcccacacg ccgaggaagc tgcggcagcc catcggagtt ttaaacgaac
    12481 cggtgacttt cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc
    12541 actccagcgc cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat
    12601 cgcgaatcgc aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg
    12661 cgatacggtt acgcaggata gattcctgaa tcaggcgcga ctcccagctg gtgcccagac
    12721 caaaaccaaa ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt
    12781 actccagcga ctggaaggca tcgtagcact ctttaatggt acgagagtta gcgtagcctt
    12841 cggcgtccgg gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtga
    12901 caaacggtgc cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg
    12961 cgacctgagc aataatctgc gcagttacag cggttgccgt agagatcgac ttcggcggtt
    13021 caatctcggc gttccccatc ttaaagccct gggtcagcat gcctttcagg tcgatcaaca
    13081 tgcagttgaa catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac
    13141 gctcatgtgc ctgcaccacg tcacgcggca gcaggtgctg acgtgcatag tgtttagcca
    13201 cgatcccggc cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt
    13261 cgttgagtaa cgaggcgttg gtctgctcga ccagaccacg gatctcctga ttcaggcgac
    13321 cgcgtttttc acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt
    13381 gtttgtatgg acctgacatc agctggtttt caacagcggt ctggatctca ttgatatcca
    13441 cctggttgcg gccctgcatc tgctcgctga caactgcagc aacagtggcg caataatcgg
    13501 catcatcgac ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt
    13561 taaacggcac tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt
    13621 tttaagaaca ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt
    13681 ttccggtgct ttccgcaatc gccatccgct tttccacaac gccgcacctt tttcgtgggg
    13741 agctgtagta gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag
    13801 tctatatata gtgctttgca tcaaggatgt ttgaactttt tttgatgtag ctcaaagtaa
    13861 aaagcagagc gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc
    13921 tggttgaaaa ataaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt
    13981 accggggctt agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat
    14041 tcatggcaca gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc
    14101 cgcgcatttg ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca
    14161 aggtttgccc cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat
    14221 cctggtaatt gccccatgtc aggatggctt cctgcttacg cagtgcgatt aacttttggt
    14281 aggtgtaaaa caccgaggaa tcatcggcca gcgcggcttc tacgttgatt tgttgatagt
    14341 tatcacccag gccaatccac ggttcgccag ccgtaaatcc ggcattatcg ccgttgctcc
    14401 attgcatggg cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt
    14461 cggcatcacg accatcgttg cgcagctcgg caaacatatt gaggctctct acgtcgcgat
    14521 agtcagtaat gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat
    14581 acggcgttcc ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt
    14641 attcaccttc atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca
    14701 aggcattcca tgctacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg
    14761 ccacaaagtc aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt
    14821 gatgaaaatt aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat
    14881 gctcaaggct ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat
    14941 cgcggttcat ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc
    15001 cgtcgccatc caggtcatca gggaaacgcg ggtctttgga gatcagattc accacatcca
    15061 ggcgcaaccc gtcgacccca cgatcggccc agaactcaca gacttttttc agcactgcgc
    15121 gtaccgctgg attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt
    15181 actgttcgct ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt
    15241 tcggtggcgt ttctggttct ccatcgcgcc agatataaaa ctggcggtaa gggctttctt
    15301 tgttcagcgc ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga
    15361 gaatgatacg aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg
    15421 tgccgtaggt gggatcaatc gccgtatagt tcgctacgtc gtaaccgtta tcgacctggg
    15481 gagagacata aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca
    15541 ggcgttggat aacgccacgt aaatcgccgg taccgctacc cgtggtgtcc tgaaaactct
    15601 ttggataaat ctggtagata acgccgtttt gccaccagtg gggaggatta gtcattacgt
    15661 tattcctgca aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc
    15721 gtgcccaggc ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg
    15781 gcgatagcca ttgccagcgc aaacacctgc cagtagttcg gttgaatcga gagaattccc
    15841 ggcaggccgc ctacgccgat gccattcgcc ataacgccgt tcaggccgca tagcaatcct
    15901 gccagaccag aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg
    15961 tacattgcag gctcagtgac cccaagccag gcggagatcg ccgcaggcac ggagatctcg
    16021 cgttcattgt gcttgcggct ggaaatgata atgccgatca cggcggagcc ctgagcgata
    16081 ttcgacagcg caatcagtgg ccacactggc gtaccaccca tgctttgaat catctgcaaa
    16141 tcaatagcaa gcgtggtctg gtgtaccccg gtgatcacca gcggggcgta caggaagcca
    16201 aacaatgccg cgccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca
    16261 acgccatcgc caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg
    16321 aggatcagcg aacagacggg caccaccacc agatagaggt aatctggcac gatgcgttta
    16381 aggcgagttt caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc
    16441 tgatagccca ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc
    16501 ccgagcagat aagcgttcat cagttggggg gaaaccagtg tcacgccaag cacgatacca
    16561 aggatcggtg tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag
    16621 aagaagatcg cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac
    16681 atttgcgcca gcgtttgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg
    16741 aggatcaaac cgccgctaat caacgcgggc agcaacggga agaaggtctc tgcgaaatga
    16801 gagatcaact gctcatgcca tttcatattc tgccgggcgg ctttttttac ctgctcttta
    16861 tcaacctgcg cctgtccggt tgacgctatc agtgcttgat agtagtcacc cacgttggtg
    16921 ccaatcacca cctgaaattg cctggcattg gtgaaacagc ctttcaccat ggggagttgc
    16981 tcaatttctt tcggtctggc attggccggt tggttgagga caaagcgtag gcgagtaata
    17041 cagtggctca ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata
    17101 tccgtttggt ttattttgct catcataaag ccccatggca gatgacattt ttggttggct
    17161 gcagaatata ttgcgcggaa aaatttaaaa acgggaacgt tccctaaacg cagcgaagat
    17221 cacaatttat cgttcaggaa acgatctgga cagggtggcg gggatgatga tttgttgcgg
    17281 ttcgctgcgc ccggttacct gtgcgatcaa ctggcaggcc gcctggcgtc cagcttcggc
    17341 gtaaccggga tctacggtga cgatctccgg atggaggaat ttcattaacg gcgtattacc
    17401 gacgctcgcc agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag
    17461 tgccagcgtg tcggttgcgc acagtaaggc ggtagtttca ggcgtaatca cttttgctac
    17521 gttctcatag ccttgcttca tagcaaggcc tggcagagcg gcaacgggat gcagtttatg
    17581 cgctttgcag aacgccaggt aggcttcgtg acgtcgctta ccggtcgtca cgtcactgtg
    17641 cggcacgccg agataactga tattacgatg cccctggtca tacagccgtt gcatcaggat
    17701 cttgattgcc ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag
    17761 cagaaccagc gatgactgcc agtgggctaa catttcttct gttatgccgg taaaaccgaa
    17821 cagcactacg ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg
    17881 cggggaaaac tgactttcca tcatgattgg gtcgtaacct tgttcataga actctggcag
    17941 catggtttga acggcgagat tttctgacaa cgaatccagg cgggtaacaa tgatggcgac
    18001 cactttatcg ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg
    18061 attcatcact gcttcaacac gctcgcgggt gcgctggctc acgccgcttt cgttattcag
    18121 cacccgggaa actgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag
    18181 ccgattttgc atcctgttgt cctgtaacgt gttgtttaat tatttgagcc taactttacc
    18241 cgtgcattca gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta
    18301 tatcgccata gatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc
    18361 acttaccgga ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact
    18421 tttccggtaa gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaac
    18481 gtccctgctc agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt
    18541 actgtttcag acagtgcgga gggactcctt atgtttaaag aaatttttac ccggctcatt
    18601 cgccatttac cttcccgtct ggttcatcgt gatccattgc ctggcgcgca gcagacagtg
    18661 aatacggtgg tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa
    18721 gaagaattgt ggaaaacgtt cgacacccat ccggaagggt taaatcaggc ggaagtggaa
    18781 tctgcccgcg aacaacatgg tgaaaataaa ttacccgcac aacaactgtc gccgtggtgg
    18841 gtacatttat gggtctgcta tcgcaacccc tttaatattt tactcaccat tctcggcgct
    18901 atttcttacg ccacggaaga tttatttgct gcgggcgtta tcgcgctaat ggtcgctatt
    18961 tctacgttgc tgaactttat tcaggaagca cgttccacta aagcggcaga tgccctgaaa
    19021 gcgatggtca gcaataccgc gacggtgctg cgcgtaatta acgacaaagg cgaaaatggc
    19081 tggctggaga tcccgatcga ccagctggtg cccggcgata ttataaaact ggcggcgggg
    19141 gatatgatcc cggcagattt acgtatcttg caagcgcggg atctgttcgt cgctcaggcg
    19201 tcgttaaccg gtgagtctct gcccgtggaa aaagccgcta ccactcgcca gccggagcac
    19261 agcaatccgc tggagtgcga cacgctgtgt tttatgggca ccaccgtggt gagcggcacg
    19321 gcacaagcaa tggtgattgc tacaggtgcc aatacctggt ttggtcaact ggcggggcgt
    19381 gttagtgagc aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgtcagtatg
    19441 ctgctgattc gctttatgct ggtgatggca ccggtggtgc tgttaatcaa tggctacacc
    19501 aaaggcgact ggtgggaagc ggcgctgttt gcgctttcgg tcgcggtagg cctaacgccg
    19561 gaaatgttgc cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa
    19621 cagaaagtga tcgtcaaaca tctggatgct attcagaact ttggcgcaat ggatattctg
    19681 tgcactgata aaaccggcac cctgacgcag gataaaattg tgctggagaa tcataccgat
    19741 atctccggta aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattatcag
    19801 accggactta aaaacctgct cgatacagcg gtgctcgaag gtacggatga agagtcagcg
    19861 cgctcgctgg ccagtcgttg gcaaaaaatc gatgagattc cgtttgattt cgagcgtcgc
    19921 cggatgtcgg tggtagtggc agaaaatacc gagcaccatc agctggtttg caaaggtgca
    19981 ttgcaggaaa tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtgccgctc
    20041 gatgacatca tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg
    20101 cgcgtggttg cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg
    20161 gatgaatccg acctgatcct cgaaggatat attgcttttc ttgatccgcc aaaagagaca
    20221 accgctccgg cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc
    20281 gacagtgagt tagttgcagc gaaagtgtgc catgaagtgg ggctggacgc gggagaggtg
    20341 gtcattggta gtgatattga aacgctatct gacgacgaac tggcaaatct tgcacagcgc
    20401 accacgctgt ttgcccgcct gacgccgatg cataaagaac gcattgtgac cttactgaag
    20461 cgcgaagggc atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc
    20521 gcggcggata tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat
    20581 atcatcctgc tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc
    20641 actttcgcca acatgctgaa atacatcaaa atgacggcga gttctaactt tggtaatgtg
    20701 ttcagcgtgc tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg
    20761 cttattcaga acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac
    20821 gacgagcaaa ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatgatc
    20881 ttcttcggac cgatcagctc gatcttcgat attctgacgt tttgcctgat gtggtgggta
    20941 ttccatgcca acacgccgga aacgcaaacg ctgttccagt cgggatggtt tgtggtgggc
    21001 ttactgtcgc aaacgctgat tgtgcatatg atccgcaccc gccgtgtgcc gtttattcag
    21061 agctgtgcat cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca
    21121 ttgccgtttt caccgctggc cagttatctg caattacagg cgttgccgtt aagctatttc
    21181 ccgtggctgg ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaaggattt
    21241 tatagccgtc gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga
    21301 ttgcagacaa agtgagtttt gttcatgccg gatgcggcgt aaacgcctaa tccggcctac
    21361 aaattcaatc aattgcagga atcacgtagg cctgataagc gtagcgcatc aggcgatttt
    21421 gcgtttgtca tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac
    21481 gaatagcgat cgcttcgatc tcaatcttta cgtctttcgg cagacgggca acttcaacgc
    21541 aagaacgtgc cgggaaggtg gcgttgtgtt cggtgaagaa ggtttcgtaa gtggcgttta
    21601 cggttgcgaa gtcgttcaga tcttttacaa acacggtagt tttaacgatg tcgcccactt
    21661 tcagaccagc ggcttcgacg atcgctttta cgttatccag cgactgacgt gcctgtgcag
    21721 cgacgtctgc cggtacttcg cccgttttcg gatttaccgg gatctgaccg gaggtgatga
    21781 tcatatttcc cagatcaacg ccctgtacgt aaggaccgat agctgctggt gcattttccg
    21841 tcgcgatagt tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta
    21901 tagggagcca gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa
    21961 actctttttc acagtatttg catttgagcg cgatgtcatt ggcgcgtttt cgcacggcaa
    22021 agctggatga aaccggttcg gcatggctga tacagttgct gttcgggcag accaacacat
    22081 tgtcgatgcg ctctggcaga ctggggcgcg atttacccac cacttcatag ttgtcgatac
    22141 ggttaaccgt ggcttgcggc gcatacaatg ccagttgatc tacctgatct tcactcaaaa
    22201 aggtattttc gattttgatc agatctttgc ggcccatctc gccagaaggc aggttcagac
    22261 caatggtgat gcgctgatcc gtttcggtca gcttgaacag actcaacagc ttaaaaccga
    22321 tctgggcggg gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat
    22381 tatcgtgtgt catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca
    22441 gtaacgcctg gcgagcgaaa atcccgttgc ctgcctgctg gaagtaccag gcgtgtggcg
    22501 ttttatcaac atccgtcgca atttcatcaa cacgcggcag cggatgcagt actttcatat
    22561 tggctttggc gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt
    22621 attcggatgg gtccaggcgc tctttttgca cgcgggtcat atacaggatg tctacttccg
    22681 ccatcacttc ttcaatagag ctgtgcagac tccatgcgat ccctttttca tcgagcatat
    22741 ccagaatgta ttgcggcatt gccagcgcgt ccggcgcgat gaagtaaaaa cggttgccgt
    22801 cgaacttcgc taacgcctga gtcagggagt gaacggtgcg gccatatttc aggtcaccaa
    22861 ccattgcgac gtggagattg tccagacgcc cctgggtttc ctgaatagtg aataagtcca
    22921 gcaaggtttg cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc
    22981 cggaaaactc ggtggccagg cgcgccgcac cttcctgcgg atgacgcatc actatcgcat
    23041 cgacgtaagt gctgataacc gaaatggtat cggccagcgt ttcgcccttt ttacccagtg
    23101 atgtattggc gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt
    23161 cgaaagagag gcgggtacgg gtagaggctt cgaagaagca gctggcaatg actttgtgct
    23221 tcaacagctc tggttgcggg tttgctttca gttttgccgc tgttgccagc accagattaa
    23281 ggtcatcgcg actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca
    23341 tcttttatct cctgacgcct gggcaaaaaa aagcccctcg attgaggggc tgggaatggg
    23401 tgatcaacgg gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat
    23461 gtcgaacaca ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatcgtca
    23521 gaagcggtgc aagcaattga tgcaatattt tgtcagcgca aacggtttat ttgaatttaa
    23581 agttaaaata tatgcatttt tatttactgt gattctgggg ggaactgtcg ttcggtatca
    23641 ataattgcag acatttcctg cttttttctt tttcactttc acgcaatcag atatgcattt
    23701 tattccattc atccgactta tagggcggag tgtgaaaacg aacggctagc actattgctt
    23761 agagctcagg gatgcgcgct atcactttaa tttcaaaatc aaagcctgcc agccatgtaa
    23821 cgcccaccgc cgtccaggtt ggataagatg gcgcgctaaa tatttcattt ttcaccgtca
    23881 tgatgtcttc aaattgtttt tctggatcgg tatgaaagct cgtaacatca atgatatcgt
    23941 caaaagtgca tcccgcagct gccagggtcg catgcaaatt atcaaaggcc agtctgactt
    24001 gttgctgaaa atcgggttct ggtgttccgt cctctcgact tcctacttgc ccggaaacaa
    24061 acagcaaatc gccggaacga atagccgcag aataacgatg ctcagcatac agtgaatgtc
    24121 gtccagcagg gaaaacagcg gttctttcta ccatttggtt atcctcaaga tttacgacat
    24181 gaacagaaga tttatattta ccaggagccg cttttagcgg acgacgtgag taaacaaaac
    24241 ccagacatca tgggaaatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
    24301 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
    24361 accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa actatgcgac
    24421 atatcgcgca ttggcccgtt ggcgggatta gcgtcggtat caattggccc tggctggacg
    24481 acgttgatgg tgatcccacg cggtccgaaa tcacgggcca gcccgcgcgc catgccttgc
    24541 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
    24601 acggagccga tgattaagat gcgtccgcct tcgggcatct gccgggcggc ttcaacagag
    24661 gcatggtaag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat
    24721 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
    24781 ctcttacgaa cgacatcaat aacagcgtct ttgtcagcac tatctgtgaa tactgctgtc
    24841 gctccagtct cttgtgccag gcgttcagcg gcatctttcg accccgcata ggtgaatcgt
    24901 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc acggctacca
    24961 ccgaggatga gaactgtctt acctgtaaaa gcgcccataa ggactccttg atttattatg
    25021 taacatgcat tacaaaactg ttttaacttt ctgtcaacaa gttttgtaat ggtcactaaa
    25081 aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
    25141 gcaaaagtgc tttttcacca aaaaggtttc gatgctgtca gtgttgctga agttactgat
    25201 tatcttggta ttaacccccc gagcctctac gcggcttttg gcaataaggc tgggttattt
    25261 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
    25321 gatgatcgtc cggtagggga gtgcctggct gaggtattaa aagaagcggc gcgccgatat
    25381 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gcattcatag tcatgatcca
    25441 caagcgcgtg atattgccgt tcaatattat cacgccgcag aaacgaccat ttatgactat
    25501 atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
    25561 tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg tgcaacagct
    25621 gcactggcgg gggaagcgat aaaaactatt ctcaaggagt gatgcgggcc ttgacccgaa
    25681 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctag
    25741 cggaattgat catcttttcg tataacgatg gaaatgaaac gttgttttaa ttaaggagtg
    25801 gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc
    25861 caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcacgat
    25921 atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gcacgaagct
    25981 cgccgtgcgg agtaccatgc ccgctatctc gacattcaga ttgtgttaaa aggtcaggaa
    26041 ggcatgacct tcagcacgca acctgcaggc accccggata ccgactggtt agctgataaa
    26101 gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatccttaa tgaaggtgat
    26161 tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc
    26221 cgggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac
    26281 tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat
    26341 ttgtaatgct tagacgttct tattaactca aggagttcgt catgagcaaa atatcagatt
    26401 tgaattattc tcaacacatt acattagccg acaattttaa acaaaaaagt gaagttttaa
    26461 atacctggcg tgttggaatg aataattttg cccgtaatgc cgaggggcag gataacacaa
    26521 gaaatatcct tgatcctaag acatttttgg aatttttggt aaaaatattt actctaggtt
    26581 atgtggattt tagcaaacgc tccaacgaag cgggaagaaa tatgatggct catattgagt
    26641 cctcatctta tatcaaaaat aatgatggca gtgagataat aaagtttgtt atgaataatc
    26701 ctgaagggga acgagcggat ttatctaagg tggaaataga aatcacactt tccactttca
    26761 ctactatggg gactcgtcaa ggacatacgg ctattatatt tcaacaacct gatggttcga
    26821 ctaatcgtta tgaaggaaag tcctttgaaa gaaaagatga gagttcatta cacctgatta
    26881 ctaacaagat tctggcatgt taccaacgcg aagctaacaa ggaaatagct cgtctattaa
    26941 atatttcaca ggagttaaat aattctcagg acttgaataa ctcgcaggtg agttgtaaag
    27001 attcagttga ttctacgatt acggatttat tagaaaaacc attgaataat gcattattag
    27061 caataaggaa agaacatctg ctattaatgc cttatgtatg taatgaatcg atttcatact
    27121 tgcttggtga aaaagggata ctgaaagaaa tagatgacct taacgcagta aataattatc
    27181 ttcttaataa taagaaagct actgacaatg aaattaatga catcaaagtt aacctgtctc
    27241 atattcttat tgattcctta gatgatgcaa aagttaacct tacaccggtc atcgattctg
    27301 gagacttttt taaaatcccc atatattaat gatgtaagaa tactggattg gtgttttaat
    27361 aaaagaatgc aatattttgg tgattctgaa aagataaagt atgcatgctc cgtgataaat
    27421 catattgatt ttagccgcga tcaatctaaa gattttagct gcgatcaatc taaaattaaa
    27481 atagctgaga ccttattttt caatctcgat aaagaaccct ataaaaa
//
LOCUS       DAJVOY010000038        27355 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVOY010000038 DAJVOY010000000
VERSION     DAJVOY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.038"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            435..3620
                     /gene="rne"
                     /locus_tag="OQL90_001428"
     CDS             435..3620
                     /gene="rne"
                     /locus_tag="OQL90_001428"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1368251.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(3817..4770)
                     /gene="flgL"
                     /locus_tag="OQL90_001429"
     CDS             complement(3817..4770)
                     /gene="flgL"
                     /locus_tag="OQL90_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS1368252.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(4782..6425)
                     /gene="flgK"
                     /locus_tag="OQL90_001430"
     CDS             complement(4782..6425)
                     /gene="flgK"
                     /locus_tag="OQL90_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCS1368253.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(6491..7432)
                     /gene="flgJ"
                     /locus_tag="OQL90_001431"
                     /pseudo
     CDS             complement(6491..7432)
                     /gene="flgJ"
                     /locus_tag="OQL90_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(7432..8529)
                     /gene="flgI"
                     /locus_tag="OQL90_001432"
     CDS             complement(7432..8529)
                     /gene="flgI"
                     /locus_tag="OQL90_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1368254.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVSGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(8541..9239)
                     /gene="flgH"
                     /locus_tag="OQL90_001433"
     CDS             complement(8541..9239)
                     /gene="flgH"
                     /locus_tag="OQL90_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1368255.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(9292..10074)
                     /gene="flgG"
                     /locus_tag="OQL90_001434"
     CDS             complement(9292..10074)
                     /gene="flgG"
                     /locus_tag="OQL90_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCS1368256.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(10212..10967)
                     /gene="flgF"
                     /locus_tag="OQL90_001435"
     CDS             complement(10212..10967)
                     /gene="flgF"
                     /locus_tag="OQL90_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCS1368257.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(10987..12195)
                     /gene="flgE"
                     /locus_tag="OQL90_001436"
     CDS             complement(10987..12195)
                     /gene="flgE"
                     /locus_tag="OQL90_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1368258.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(12220..12915)
                     /gene="flgD"
                     /locus_tag="OQL90_001437"
     CDS             complement(12220..12915)
                     /gene="flgD"
                     /locus_tag="OQL90_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1368259.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(12927..13331)
                     /gene="flgC"
                     /locus_tag="OQL90_001438"
     CDS             complement(12927..13331)
                     /gene="flgC"
                     /locus_tag="OQL90_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCS1368260.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(13335..13664)
                     /gene="flgB"
                     /locus_tag="OQL90_001439"
     CDS             complement(13335..13664)
                     /gene="flgB"
                     /locus_tag="OQL90_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1368261.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            13820..14479
                     /gene="flgA"
                     /locus_tag="OQL90_001440"
     CDS             13820..14479
                     /gene="flgA"
                     /locus_tag="OQL90_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1368262.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            14555..14896
                     /gene="flgM"
                     /locus_tag="OQL90_001441"
     CDS             14555..14896
                     /gene="flgM"
                     /locus_tag="OQL90_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1368263.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSTS
                     TSTSTSTSTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADAL
                     INEAQQDLQSN"
     gene            14901..15317
                     /gene="flgN"
                     /locus_tag="OQL90_001442"
     CDS             14901..15317
                     /gene="flgN"
                     /locus_tag="OQL90_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1368264.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(15357..16892)
                     /gene="murJ"
                     /locus_tag="OQL90_001443"
     CDS             complement(15357..16892)
                     /gene="murJ"
                     /locus_tag="OQL90_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1368265.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(17059..17925)
                     /locus_tag="OQL90_001444"
     CDS             complement(17059..17925)
                     /locus_tag="OQL90_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1368266.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(17927..18574)
                     /locus_tag="OQL90_001445"
     CDS             complement(17927..18574)
                     /locus_tag="OQL90_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS1368267.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(18585..19169)
                     /gene="rimJ"
                     /locus_tag="OQL90_001446"
     CDS             complement(18585..19169)
                     /gene="rimJ"
                     /locus_tag="OQL90_001446"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1368268.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            19405..20613
                     /gene="mdtH"
                     /locus_tag="OQL90_001447"
     CDS             19405..20613
                     /gene="mdtH"
                     /locus_tag="OQL90_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1368269.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            20677..21324
                     /gene="grxB"
                     /locus_tag="OQL90_001448"
     CDS             20677..21324
                     /gene="grxB"
                     /locus_tag="OQL90_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1368270.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            21458..22018
                     /locus_tag="OQL90_001449"
     CDS             21458..22018
                     /locus_tag="OQL90_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1368271.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            22124..23170
                     /gene="pyrC"
                     /locus_tag="OQL90_001450"
     CDS             22124..23170
                     /gene="pyrC"
                     /locus_tag="OQL90_001450"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1368272.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            23244..23489
                     /gene="dinI"
                     /locus_tag="OQL90_001451"
     CDS             23244..23489
                     /gene="dinI"
                     /locus_tag="OQL90_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1368273.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            23779..24033
                     /gene="bssS"
                     /locus_tag="OQL90_001452"
     CDS             23779..24033
                     /gene="bssS"
                     /locus_tag="OQL90_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1368274.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            24148..25266
                     /gene="solA"
                     /locus_tag="OQL90_001453"
     CDS             24148..25266
                     /gene="solA"
                     /locus_tag="OQL90_001453"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1368275.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCTGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            25314..25427
                     /locus_tag="OQL90_001454"
     CDS             25314..25427
                     /locus_tag="OQL90_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCS1368276.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            25688..26254
                     /locus_tag="OQL90_001455"
     CDS             25688..26254
                     /locus_tag="OQL90_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1368277.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            26258..26833
                     /locus_tag="OQL90_001456"
     CDS             26258..26833
                     /locus_tag="OQL90_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1368278.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGNDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(26875..>27355)
                     /locus_tag="OQL90_001457"
     CDS             complement(26875..>27355)
                     /locus_tag="OQL90_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1368279.1"
                     /translation="KDKKIVMYCTGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKA
                     REQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCP
                     VCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
BASE COUNT         6569 a         7288 c         6889 g         6609 t
ORIGIN      
        1 actgtgagta agcgggtgat aaatggtaaa agtcatcttg ctataacaag gcttgcagtg
       61 gaataatgaa gccgtttccg tgtccatcct tgttaaaaca agaaatttta cggaataacc
      121 cattttgccc gaccgatcat ccacgcagca atggcgtaag acgtattgat ctttcaggca
      181 gttagcgggc tgcgggttgc agtccttacc ggtagatgga aatatttctg gagagtaata
      241 cccagtctgt ttctttgata attgcgctgt ttttccgcat gaaaaacggg caaccgacac
      301 tctgcgcctc tttgagctga cgataaccgt gaggttggcg acgcgactag acacgaggcc
      361 atcggttcac acccggaaag gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata
      421 atgagtaagt tacgatgaaa agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg
      481 ttgcccttgt agatgggcag cgtctgtatg acctggatat cgaaagtcca gggcacgagc
      541 agaaaaaggc aaacatctac aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg
      601 cttttgttga ttacggcgct gaacgtcacg gtttcctccc actaaaagaa attgcccgcg
      661 aatatttccc tgctaactac agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg
      721 aaggtcagga agtcattgtt cagatcgata aagaagagcg cggcaacaaa ggcgcggcat
      781 taaccacctt tatcagtctg gcgggtagct atctggttct gatgccgaac aacccgcgcg
      841 cgggtggcat ttctcgccgt atcgaaggcg acgaccgtac cgaattaaaa gaagcactgg
      901 caagccttga actgccggaa ggcatggggc ttatcgtgcg caccgctggc gtcggcaaat
      961 ctgctgaggc gctgcaatgg gatttaagct tccgtctgaa acactgggaa gccatcaaaa
     1021 aagccgctga aagccgcccg gccccgttcc tgattcatca ggagagcaac gtaatcgttc
     1081 gcgctttccg cgattactta cgccaggaca tcggcgaaat ccttatcgat aacccgaaag
     1141 tgctcgaact ggcacgtcag catatcgctg cattaggtcg cccggatttc agcagcaaaa
     1201 tcaaactgta caccggcgag atcccgctgt tcagccacta ccagatcgag tcgcagatcg
     1261 agtccgcctt ccagcgtgaa gtccgtctgc cgtccggtgg ttcgattgtt atcgacagta
     1321 ccgaagcgtt aactgccatc gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg
     1381 aagaaaccgc gtttaacacc aacctcgaag ctgccgatga gattgctcgt cagctgcgcc
     1441 tgcgtgacct cggcggcctg attgttatcg acttcatcga catgacgcct gtacgccacc
     1501 agcgtgcggt agaaaaccgt ctgcgtgaag cggtgcgtca ggaccgtgcg cgtattcaaa
     1561 tcagccatat ttctcgcttt ggcctgctgg aaatgtcccg tcagcgcctg agcccatcac
     1621 tgggtgaatc cagtcatcac gtttgtccgc gttgttctgg tactggcacc gtgcgtgaca
     1681 acgaatcgct gtcgctctct attctgcgtc tgatcgaaga agaagcgctg aaagagaaca
     1741 cccaggaagt tcacgccatt gttcctgtac caatcgcttc ttacctgctg aatgaaaaac
     1801 gttctgcggt aaatgccatt gaaactcgtc aggacggtgt gcgctgcgtg attgtgccaa
     1861 acgatcagat ggaaaccccg cactaccacg tgctgcgcgt gcgtaaaggg gaagaaaccc
     1921 caaccttaag ctacatgctg ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag
     1981 aagagttcgc tgaacgtaag cgtccggaac aacctgcgct ggcaaccttt gccatgccgg
     2041 atgtgccgcc agcgccaacg ccagctgaac ctgccgcgcc tgttgtagct ccagcaccga
     2101 aagctgcacc ggcaacacca gcagctcctg cacaacctgg gctgttgagc cgcttcttcg
     2161 gcgcactgaa agcgctgttc agcggtagtg aagaaaccaa accgaccgag caaccagcac
     2221 cgaaagcaga agcgaaaccg gaacgtcaac aggatcgtcg caagcctcgt cagaacaacc
     2281 gccgtgaccg taatgagcgc cgcgacaccc gtagtgaacg tactgaaggc agcgataatc
     2341 gcgaagaaaa ccgtcgtaat cgtcgccagg cacagcagca gactgccgag acgcgtgaga
     2401 gccgtcagca agctgaggta acggaaaaag cacgtaccac cgacgagcag caagcgccgc
     2461 gtcgtgaacg tagccgccgc cgtaacgatg ataaacgtca ggcgcaacaa gaagcgaagg
     2521 cgctgaatgt tgaagagcaa tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg
     2581 ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt gcgttacgag caaagcgtag
     2641 ccgaagaagc ggtagtcgca ccggtggttg aagaaactgc cgctgccgaa ccaattgttc
     2701 aggaagctcc agctccacgc acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa
     2761 ctgcaccaga acagcaagaa gagaacaatg ctgataaccg tgacaacggt ggcatgccgc
     2821 gtcgttctcg ccgctcgcct cgtcacctgc gcgtgagtgg tcagcgtcgt cgtcgctatc
     2881 gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg
     2941 aactggcctc tggcaaagtc tggatccgct atccaattgt acgtccgcaa gatgtacaag
     3001 ttgaagagca gcgcgaacag gaagaagtac aagtgcagcc gatggtgact gaggtccctg
     3061 tcgccgccgc tgtcgaaccg gttgttagcg cgccagttgt tgaagaaatg gctgaagtcg
     3121 tagaagcccc cgtaccggtt gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg
     3181 aagtgattgc cgccgcggta actgaacagc cgcaggtgat taccgagtct gatgttgccg
     3241 tagcccagga agttgcagaa cacgcagaac cggtggttga accgcaggaa gagacggcag
     3301 atattgaaga agttgccgaa actgctgagg ttgtggttgc agaacctgaa gttgttgctc
     3361 aacctgccgc gccagtcgtc gctgaagtcg cagcagaagt tgaaacggta gccgcggttg
     3421 aaccagagat caccgttgag cataaccacg ctaccgcgcc aatgacgcgc gctccggcac
     3481 cggaatatgt tccggaggca ccgcatcaca gtgactggca gcgccctact tttgccttcg
     3541 aaggtaaagg tgccgcaggt ggtcatacgg caacgcatca tgcctctgcc gctcctgcgc
     3601 gtccgcaacc tgttgagtaa taattagctc aaagtaatca agccctggta actgccaggg
     3661 cttttttatt tcatgtttga atcaacatat taaaattcat caaatggcat ccttgctaac
     3721 caacaatgca aaataggcaa aaaaaatgcc cgccccggcg atccctgagc gggcagacaa
     3781 aacataccca gtttcatgat atgttttaaa gcgagcttat ttgttgagct ggaagagcga
     3841 caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt gctgcatgat
     3901 gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct gctgcgtttg
     3961 ccctaaagcg cgatcgctac ctaaggaatc cagcgactcc agttcgttca gctgcgtgcc
     4021 taattccgcg cgaacggcaa ggacattatt cagcgagttt ttcagtccac ggttggtttt
     4081 atccaatgcc gcagcggcga tttctttatc cgcttcgcta tccgcgaccg gcgttttcag
     4141 ggctgcgatg gcgctatcca gcatggcaaa aagattggtt tcagaagcgc taccgtctgg
     4201 ctccgcaact gcgttgctgg taatactgtc gaaaattttg tcacccgtgt gccctatcac
     4261 catcgaacgc gaagcatcga cctgttgctt aatactttct gctccaccga cgtatttccc
     4321 tttctcttcg ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt agcgcccgtt
     4381 accgtcagtg gtgttcgcca gattcagcaa ctggtcacga agcccctgaa tatccgtagc
     4441 cagcgaggcc cggtcatcgt cactcaaggt gccattgctg gcgtagacaa ttttttcctg
     4501 agcattctgg atagcagtgg tgacctggct aagtacactc tcttccagtg acactttttg
     4561 aatggcgaaa gtacgcgcca gcgtgtactg gctgttttgc gcctgtgcct gggagagaac
     4621 tacggcttgt gatgctgcaa tgggatcgtc agaagggtta acgactcgct tacctgtcga
     4681 catctgctcg ccgtacttca tccattccgc ctgagaattg gtgatgccac gcatgttttg
     4741 ctggtacatc atctgggtac tgaaacgcat cttatctccc cttagcgaat gttaatcagc
     4801 gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta atactgctga
     4861 aaacgttgca gatttccgta ctcttcgtcg agattgacgc cagagataga ctgctgctga
     4921 ttggaaagct gcgtcaccac attaccttgc gtggcgctac tggttttcaa cgtcgcggtt
     4981 ttattaccga tatcactcac taacgaggca taagcgtcgt taaaggattt cgcaccgccc
     5041 accgttttac tgttgctttg cagatccagc agggcctgac cgttgcggtt atcgctatca
     5101 cccgcatctt tttcgctcgc catcgctatt ttcgcttcgt cggtgattaa tacatccatg
     5161 ttgacgatgg cgtcacttac tggtttcagc gtgaagctgt cgttaacggc aggcgttcct
     5221 gtaaacgtca actccagacc atcaaatgcc actttaccgt tggcatccgg cgtcaccgta
     5281 aaagtggtat tgctggcaag gcgggtgacc tgccactgat tattatcgaa cgagattttg
     5341 taatctgtcg ccagtaccgc ggaggcatca gttaccgtgg caccgatcgc aacgtcacct
     5401 ttgtttttcg tgttttgcaa aaccgcgggc ttaccgatag caaagaaatc ttcaccagca
     5461 tcgccgttgg catcaaatcc ggctttgtgt tgggtgttga aagcctcggc aaatgccagc
     5521 gccagttgtc caagcgtatt acgcgtctgg tccagatcct gagaacggaa tgtcagaatg
     5581 ccgcccagcg acccggtatt cagtaatttc tccgggatct caatattgcc tgccgtccca
     5641 tcaacgtaag cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc cgccagttgc
     5701 cgcgccgtac ttccctgaac cagtgagtaa ccattggcca tcgtgatgtt ataagtgccg
     5761 ccatcctgaa cgctgacttc tacaccaaca atctggttta attcgctcac cagttgatcg
     5821 cgttgatcca gcagattgtt aggtgacgcc cctgccccca cgcctgtcag gcgggagatt
     5881 tggtcgttca ggctggcgat ttgtttagcg taattgttga tctgatcaac gctggcacct
     5941 atcgcgatat tgacctgttt gtcctggtca cgaagatact gatcggtggt tttaaactga
     6001 ttcaccaacc cttctgattt cccaatcagc gcctggcgcg ctgccgggtc ttccgcgtta
     6061 ctcaccagcg tttgcaggct ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta
     6121 ctggtggaga gcatattgtc gattttcgac atctgctcat agcgggcagt caggccgcta
     6181 ctttgcgtct gcgccgcacg taactggttg gtgataaacg catcatactc acgctgcaca
     6241 ccagaaacgt agacaccatt gccaacccag ccgccagcgc ccaacgtgct attggcctgc
     6301 gccataatag tggtttggcg ggtatatccg gcaacgttat agctggagat attattactt
     6361 gccgtattta acgccgcctg ggccgcgttc agtccgctca tggcgttatt aatcaagctg
     6421 gacatggagg ttccttataa gccttcaatt acagagtatt atcggcagcg acccgccgga
     6481 cttgagttat tcaaaacaga ttatcaatgt tcatactgta ggttttgctc accttgtcgc
     6541 ttatcgattt catctgctga atcatgttgg tgagtttgcg ggcatagtga ggatcggtgg
     6601 catagcccgc gtcctgtagg gcctgcgccc cctgctccgc actcgtggcg gtggtcacgg
     6661 cggcgtagcg cgggttacgc gttaacagcc caacgtaatc cgactatgct tccagatacg
     6721 agctgtagac ccgaaacttc gcttttactt tcttcgcttc gccgttttca tattcagtcg
     6781 tggtgatttc agtaactggc cctttccagt tgccagaggc tttgacacca aacaggttat
     6841 agctcggctc gccgttttcg cggcggattt gccgttgccc ccaaccagat tccagcgccg
     6901 cctgagcgag gatcaaatga tgtggcacac cgctttgctg gcttgccagt tgggcgggca
     6961 gcgagagttg cgcgaggaat gctttactgt cacccggcag cgaatcatcg tagttacgtg
     7021 gcacggcctt ttgcaccagc tgcgaaagcg cctgattttg ataacgcacc acagtttcga
     7081 gcgggaattt catcggtgct gctggcgtgg actcctctgg caatggttgt tctggcgtca
     7141 tctgtttaac catcatctct gcaagcccca gacctttgcc cgccgtcatc tgttgggcaa
     7201 tctgctggtc atacatactg gtatacaggc gagtgtgctc gctgctgaac aggccatctt
     7261 ttggtaaagc gtcgcgcatg cttttcaaca tcatctgcac gaacatccct tccacctgac
     7321 gggccaccgg acggatattt gccgccggat cttcgcccgc tttcgccttt agttcgttga
     7381 gtgattgcgc atcccaggcc gcacttgcca gtagtttgct atcgctgatc atcagatgat
     7441 ttccagtttt gcccgcagac atcccgcact ttgcattgat tgcagtatgg acatcagatc
     7501 catcggcgta gcgcccagcg cattgagcgc gcgcaccacg ttattgagcc tggcgctgga
     7561 acgtacgctt tgcagcgaac cgccgctctg gcgtaaatcg atctgcgttt gtggagtaac
     7621 cacagtctgt ccaccaccaa acggtgtatc tggctggctg acattggcct gacgattaac
     7681 tgttactgag agattcccct gcgctaccgc gcagctgtcg agggtcactt cgcgattcat
     7741 caccaccgaa ccggtgcgcg agttaatcac tactttagcg tcctgcgggg tgacattaac
     7801 ctgcatattc tggatatcgg caaggaagcg gacctgggaa ctgttgccac tcggtacgcg
     7861 cacctgaata gtccgcgcat ctaacgcggt ggcgctgcca tatccacgca cgcggttgat
     7921 ggtgtcagcg atttgctgcg ccatgctgaa atcttcgtcg ttaagttgca aattaagggt
     7981 attcccgacg ccaaactggc tgggcaattc acgttcaata accgcaccat tggtgatccg
     8041 tccaccgttc agttggttaa cctgaacact gctaccgcca gcggaggctc ctgcgccgct
     8101 aaccagaata ttgccctgcg ccagcgcata cacctgactg tcaacgccct taagcggtgt
     8161 catcaacaac gtacctccac gcaagctttt ggcattaccc atggaagaaa ccaccacatc
     8221 gatggtttgc ccctgacgtc caaacggagg aagtgacgct gtcaccatta ccgcagcgac
     8281 gttttttagc tgcatattgg tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt
     8341 attaagcgtt tgtgtggtaa acggcgtctg ggttgtctgg tcaccggtgc catccagccc
     8401 caccaccaga ccatagccaa tcagtgagtt ttgccttacc ccctgaacac tggtgagatc
     8461 gcgaatacgc tcagcctgag ccgccgtcgt gaccagtaga agaattaatg cagagagaaa
     8521 tttaatcacc acagcctcac ttacattggc gacaggttaa ggaagaaacg ctgcaaccag
     8581 cccatatttt gcgcttcgtt aatgtagcca ttgcctacgt attcaatgcg cgcatccacc
     8641 acctgagtag acggtacggt attgctgccg ctgatagtgc gtggattaac cacgccagag
     8701 aagcgaataa attcggtacc ctgattaatg gcaatctgtt tttcacccac cacatgcagg
     8761 ttgccgttga ccagtacctg gtcaaccgtc accgtcaacg tgccgctaaa ggtattgctg
     8821 gcattggccc cgccctttcc gttgaacgtg ttaccaccgg aggcttcgac atcggcacga
     8881 gcgttaccaa acagcccctg caaatagcgc ggcacagtat caaagccaaa attagtttta
     8941 ccgtcacggc tggcattcgc agaggagctt ttgctggcgc tgacgttctc ctgcaacacg
     9001 atggtcagcg tatcgccaat attgcgtggt cgacgatctt caaacagcgg ttgatagcca
     9061 tagttaatcg gctgagcaga ctggaaaata gaaccgttgg cgacgggcgt cggaccggga
     9121 accggttgtg cactggtcgc cccctgcacc agcggcgtgg agggtatcca ggcgcagccg
     9181 gttagtgaaa gcaccaacaa gctggaaatg gcataagtat gcgcagcgtt tttttgcatg
     9241 gctatcatct tcaaaaatca gtaaaccggt gaacctgcca ccggttaagc cttagagttg
     9301 cgtcagtttt tgctgcatct gatcggtggt ggacaccgct ttactgttga tttcgtaagc
     9361 gcgttgcacc tgaatcatat tgaccagttc ttccgccacg ttgacgttag acgtttcaac
     9421 atacccttga tacagcaatc ccgcgccgtt caggcccggc gtgctttcgt tcggtgcacc
     9481 agaggattgc gtttcggtgt agaggttttc gccaatgctc tccagcccgg tgtcattcat
     9541 aaaggtggtg agattgagct gcccaacctg aaccggagct gcctggcctt gttgggttac
     9601 gctgaccacg ccatcacgac cgatggtgat acttaacgca ttcgccggaa tggtgatcgc
     9661 tggctgcacc tgaaaaccac cagccgtcac cagctgcccg ttctgatcca cctggaaaga
     9721 gccgtcacgg gtataggctg atgaaccatc tggcaacatc acctggaaaa agccctgccc
     9781 tttaatcgcg acatctttgc tgttgttggt ctgcgacaga tttccctggc tgtgtaagcg
     9841 ttcagtggcg accgggcgta cccccgtgcc gatttgtaat ccggagggta aggtggtttg
     9901 ttcggaagac tgtgcccccg gctggcgaat ggtttgataa agcagatctt caaacaccgc
     9961 gcgctgacgc ttaaaaccgt tagtactgac gtttgccagg ttgttggcaa tgacgtccat
    10021 attggtttgc tgggcgtcaa ggcccgtttt ggcgatccat aatgaactga tcataaaatg
    10081 tcctgtagtg gattagtgac aagcagagca gaagcacgcg acagtgtgaa ttccccctca
    10141 ctctaaccct ctccccaaag gggcgaggga atagtccgcg cgcttttatt gcaatgtcat
    10201 caatcgttca attaactcat cgacagcagt tggttggcac ggcctgcgtt atcatcgacg
    10261 ctgctgatca ccttcatctg catttcaaaa cgccgcgcgc tggcaatcat gtcgctcatt
    10321 gccgcaacgg cattgacgtt actgccttcc agaacccccg acatcacacg caaggttgga
    10381 tctgcctgca gtaccggccc tcgcgtggcc tgggtttctg cgcttaaacg aaaaatgccg
    10441 tcgtcaccgc gctgcacttc gctgcccgtg gctttcacca gtttcagacg ccctactggc
    10501 gcaactgtat ttgccggatc gcccggattg agcgccgaga ttgtgccatc ggcagcaata
    10561 gtgatttccg ctccttccgg cacagcaatt ggccccgcct cgcctatcac ctgatgcccc
    10621 tgaattgtca gttgcccggt gggatcaacc tgaatgctgc cattacgcgt atacccttcg
    10681 ctgccgtcag cggtctgcac ggccagccag ccatcctgct gcaacgcaac gtccagcggg
    10741 cgcgaggtgt aatctatctt gccgggcgtc atgtctgcac ccggtgttga cgccgttacc
    10801 agcgtgcgcg tgggcagtga aagcccttcc accggcactg cgcgcaaggc gtttaactgc
    10861 gcgcgaaaac cgggcgtaga ggcattggcc agattactgg cggttaccgc ctgttgattc
    10921 agcgtctggc tggctgctcc catcgcagta taaattgcgt gatccattga gctatcccgt
    10981 cagcgattag cgtaagttaa ccagcgtgtt gaggatctgg tcctgggttt tgatggtctg
    11041 ggcgttagac tgatagttac gctgggcaac gatcatattg accagttctt tactgagatc
    11101 gacgttggac gcttccagcg caccgttggt cagggtgcca aagtttcccg tcccggctgt
    11161 ccccaacagc gccacgccag aagattgcgt cgcagaccag acgttgtcgc cttcggatgc
    11221 cagaccttcg ttgttggcaa agttcgccag tacaatctgc cccagcagtt gagtttgttc
    11281 gttggaatag ttgccgacaa ccgtaccgtc atcattgatt tgataactca ccagatcgcc
    11341 cggtttgtag ccgttctggg gggttgccac aatattgtta gcgccggtat tttgctgcat
    11401 ggagttgagg aagctcagac taaacgtggc gggttctgca ccgttaattg cgccggttgc
    11461 gatattattc gccatcgcac catccactaa tgtgccatta gcattaaatt ccagtgttgt
    11521 cgctgtcttc gcaatgctgt ttggatcact gctatcctgg gtgtagacct gccagttatt
    11581 atccccggtc ttcacaaagt agacgctcat gtcatgagca ttaccctgac tgtcgaaaac
    11641 agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcgctgaatg gcgtaacagt
    11701 aggaagcgga tcactggaat tcaggttgat ctgcatcgac gccgtggtgg tagttttcgc
    11761 tgccatcagg gtattcggga tcgaaatatt ggtcggattc gccccttgct gaatagtcgg
    11821 cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct tgcatattca ccaggttacg
    11881 gttttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctgtctac
    11941 cagacggaaa aaaccgttct ggctgatagc aacgtccaga cctcgcccgg tgttggtggt
    12001 cgtgccatct gtaaagtcct gagtgatacc ggcaactttt acccccagtc ccactttcga
    12061 accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt
    12121 ggcaatattg ttgccaataa catcgaggtt agtggcggca gcgtttaatc cgctaaccgc
    12181 ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc
    12241 gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgctgt tgccgcggat
    12301 cacaccctgc accagagcaa actgcagcgg ctgggcaacc agttgtgtac caccgttact
    12361 ggcggtaatc gctacattgt aagaaccgtt cggcgcagtg ctgccatcag tcaacgtacc
    12421 gtcccaggtg aaactgtgaa ctccggcggt cagttcacca atatcaatgg tgcgcacaac
    12481 cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac
    12541 accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg caagaacagt
    12601 tgtgccgggg atcatcacgc cgtgaccgat caggttactg gcctgtaacg actggctgtt
    12661 atcaatctgt ccggaaatag atccgagcat ggtattgagt ttttcaatcc cgctgaccgt
    12721 gctgatttgt gccaattgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg
    12781 gtttttcagc tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct
    12841 gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat cggtggtggt
    12901 taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcatg
    12961 cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg
    13021 ttaaccatct ctccgacaac atcaacgttc ggcattttta cgtagccctt tgcatccgcc
    13081 agcggattac ccggttcata aaccagtttg tccggggcct gactttctat aacatcggca
    13141 acctttacgc cgcctgtcgc agcacctggt gcagcgttaa cctggaatac cacctgtttt
    13201 gcccgatatg gctgtccatc gggaccggtc acgctatcag cattcgccag attactggcc
    13261 gccacgttca ggcgctggga ctgggcagtt aacgccgacc cggcgatatc aaaaatattc
    13321 agcagtgcca tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg
    13381 ctcaacgcac taaggctcat ctggtattgc agttctgcgg taggaggcgt cagcgcctgc
    13441 gccggaatgt gttgcgttga ggtcatcgtc agagcaacca cactggttgc atcccgtcca
    13501 cgttgcatga cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaagg
    13561 gtatcggcat tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc
    13621 gcctcttgtt gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta
    13681 tcaaaattct gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca
    13741 aaatgggtcg ctatttatgc cgttgatgac cattggggac gggtacaatt cacgttgtag
    13801 aaatagctgg ggggcgaaaa tgctggcaat aaaacgtagc gtggcgatca tcgcgatact
    13861 gttcagtccg ttaagtgcgg cgagcaatct cacatcgcaa ttgcacacct tttttagcgc
    13921 ccagctcgtg ggggtaagtg atgaggttcg tgtttctatt cgtacagcac ccaatctact
    13981 accgccgtgc gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt
    14041 gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac
    14101 aggaaattat gtggttgccg cgatgcccat tgtgcgggga ggaaagctgg aagctggcaa
    14161 tgtcaaacta aaacgcggac gactggatac cctgccaccg cgtacggtgc tggatatcaa
    14221 tcaacttgtt gatgccatta gcctacgcga tctatcaccc gatcaaccaa tccaattaac
    14281 ccattttcgc caggcatggc gggtaaaagc gggacaacgc gtcaacgtga tcgccagcgg
    14341 tgacgggttt agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa
    14401 tgcgcggatg cgcatggtat cgggactggt ggtcagcggc gttgttgatg cagatgggaa
    14461 tattcttata aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac
    14521 acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct
    14581 gaatcccgta agcaccgttc aaccgcgcga aaccactgac gcgccggtaa cgaacacccg
    14641 ggcggcaaaa acaaccgact ccaccagcac cagcaccagc accagcacca gcaccagcac
    14701 cagcaccagc accagcacca gtgtgacgtt aagcgacgcg caagcaaaac tgatgcaacc
    14761 cggcagcagt gatatcaatc ttgaacgtgt cgaagcgtta aaactggcga ttcgtaacgg
    14821 tgaactaaaa atggacatcg gcaaaattgc cgatgcgctg atcaacgaag ctcagcaaga
    14881 cttgcagagt aactgatcgt atgacacgtc ttgcagagat ccttgaccag atgtccgctg
    14941 tgcttaacga tctcaaaacg gtaatggatc aagagcagca acatctctct atggggcaga
    15001 tcaacggcag ccagttgcaa tggattacag aacaaaaaag ctcactgctg gcgacgctgg
    15061 attacctcga acagttacgc aagaaagaac ccaatacagc aaatagcgtt gatattagtc
    15121 aacgctggca ggaaattact gtgaaaacgc agcaactacg ccaaatgaat caacataacg
    15181 gttggttact ggaaggacag attgagcgca atcaacaggc gctggaaatg ttgaaaccgc
    15241 atcaggaacc gacgctatat ggggcgaacg gtcagacctc aacaacccat cgcggcggta
    15301 aaaagatttc gatctgaaga ttatctccgg cctgcactgc aggccggaat gcattgttac
    15361 accgtccggc gggcaaattc tttaactttg aagcccagta ccgccagtgc agcgaagtac
    15421 gcggcaatcc ccgccagcac gaccgccatt aaacgcagta agcgccaggg catggtaccc
    15481 aatgaccact ccggcatgat atgtaacata cctaaaagca cgccagacat caccagtacc
    15541 gccaccacca gacgcaacag aaacgccatc cagccgggtt gcggagtaaa gattttctgc
    15601 ttacgcaact gccagtaaag cagcgaagca ttcagacacg ccgccagacc aatagaaagt
    15661 gacagcccgg catgtttcaa cggaccaata aacgccaggt tcatcaattg cgttaaaatc
    15721 agcgtaacga tggcaatttt cactggcgtt ttaatgtcct ggcgggaata aaagccagga
    15781 gccaacactt tcactacaat caggccaatc aaacccaccg agtaggcaat taacgcccgc
    15841 tgggtcatca gcgcatcaaa cgcggtaaat ttaccgtact ggaacagcga aacggttaac
    15901 ggacctgaaa gaatgcccaa cgcaaccgca ctcggcagcg ccaacaggaa acaaagacgc
    15961 aacccccagt ccatcaaacg gttgtattca tcatgattgc cactggcaaa acttttcgac
    16021 agcgacggca gcaaaatggt gccaagcgcc acgcccagca caccggacgg aaactccatt
    16081 aagcggtcgg cgtaatacat ccaagacacc gaaccggaag caagaaacga ggcaaaaatg
    16141 gtgttgatga ttaaggagat ctggctcaca gagacgccaa ggatcgccgg tcccatctgt
    16201 ttcaccacgc gcattgctcc ggcatcgtgg aagttaatgc gcggcaggac cagcatgccg
    16261 atcttcttca ggtgcggtag ctgatacacc agctgcagga tgccgccgac cgtaacagcc
    16321 cacgccagcg ccagcaccgg tgggttaaag tacggtgcgg caaacagcgc gaaaccaatc
    16381 atgctgatgt taagcagtgt tggcgcaaac gccggaatcg agaagcggtt ccacgtattc
    16441 agaatcgctc ccaccagcga cgccagggag atcagcaaga tatagggaaa ggtaatcttt
    16501 agtagctggc tggtcagggc aaatttgtca gctgtatcag cgaagcctgg cgcggtcacc
    16561 atgatcaccc acggtgcggc gagcatgcca gcgaccgtca caaccgccag cgcaagtgtc
    16621 agcaggccag aaacataaga gacaaagacc cgcgtggcgt cttcaccctg cttactttta
    16681 tattccgcca gaatcggtac aaatgcctgg gaaaatgccc cttcggcaaa gatacggcgt
    16741 aacaagttag gaagtttaaa agcgacgaaa aaggcgtcgg ttgccatccc tgcgccaaag
    16801 attctggcga caattgcgtc tcgtgcgaag ccaagcacac gcgaaaacat ggtcatcgag
    16861 ctgacggcgg ccagcgattt taataaattc atcggtgttc taatccagac caacgggcaa
    16921 aacgcctgca agacaggcgc tgaaagaggc gaatagtcta cctggattat ggtgaattgc
    16981 taccgccaga tgttacaggg tttattcact catcgcatcg cgccagatct tgtcaacgat
    17041 acgttgcgcc agcacggcct attcgccggc ggtttgcgga actgtctgat tttgcacaca
    17101 ttcaatgaag tgccgcgcac agccgacaaa tccacgttgt tcaagagtgc tctgccaacc
    17161 aggaatcggt ttatgcacta cgccctgccc gcgctcctca cgccattcgc gcatatccgt
    17221 aatgtcgatg agcgcaccgt cagtcacggc ctgcacggtt tcacgctgac ttccggcacg
    17281 gcgatgcata caggtggtga tctgcaaagg accggccgaa aaatggtgct cggcaaacag
    17341 catttcgcca gcgtcgttag tcagtagcgt accgccatcc agagaggctt tgccgcccga
    17401 caaccacagc gcggtatcca ccacatgcag ataatcatcc agcaacgtga aataaagatc
    17461 gtgtggcccg acactattgc tacgatgttt atccattctt agcgaagctg cggtagcgag
    17521 ttgcgttttt aactcaccgt agagtggtgc gaaacgacgg ttaaaaccga ccatcaacgt
    17581 cagttttttc cgcgccgcca gttccaccag ccgttcagca tcgcgcagat tttctgccag
    17641 cggtttatcg acacagacat gtacacccgc attgagtaac gtactgacca cgtcaaagtg
    17701 gctggcggtg ctggaatgca caaaaaccgc atcgcaactg gcggcaaggc tggataacga
    17761 atcggcataa ggaatgcgcc agctttcaca aattggcagg gctttcgcgc gcgtaggcga
    17821 ccaggctcct tgtaacatcc agtcagacgc tgccgccagc accggtaacc acgctttttg
    17881 cgcaatgccg cctaatccca caacgccgat acgtaatttt ttcactttaa tctcccagat
    17941 gggccagcaa cgaatcaaga cgctgtttca gttctgccac ttcgatttcc agggcttcga
    18001 cgcgggcctg taaatcacca tcaaccgcgt tcgacatatc cgttaccgcc ggctgatcct
    18061 ctacctcacc actgaaaaga tgcatgtagc ggctttcgcg tttacccggt tcgcgggcca
    18121 gacgcaccac aaaaggacca tcttcgcgat ttgccagttg ttccagcgtc gactccaatt
    18181 ccgccatatc gctgaattca tacattcgcg cggcgcggct gcgcagttca cccggcgtct
    18241 gggcaccacg caataacaac gtggtgatta acgccacttc cgctgcgctc agtttcagat
    18301 cgccaaattc tgaattacaa aaacgttgtt catatttggt gacccgatta ccaaagccac
    18361 tcaccgtgcg cagataatgg cgtttgacca gattatccag ttgctcctgc acttcagatt
    18421 cactcagatt catgaccggt tcacggttcg ttttctgatt acaggccgtg actacaccat
    18481 tgactgagag cgggtattgc tccggcgtgg tcacctgctt ttccagcaag caaccaatca
    18541 cgcgagcttc aagggcagta agttgatatt tcatgagatt ctccttagcg gccgggcgtc
    18601 cagtctgggg tagttaatgc cgtcagtacg tgatcgcgcc attgtccatc aatcaacaga
    18661 tagtctttcg catagccttc tttttcaaaa cccagtcgcg ccagtaaatc accgctgcgt
    18721 ttattgtgcg gcatataatt agccataatg cgatgaatat gttgggtgcg ctgcatataa
    18781 cgaatggctg cggtcagggc ttcaaacatg agtcctttgc cctgccattt ttgcccaatc
    18841 gaataaccga gatagcaggc atgaaaagag ccacgaacaa cattggaaaa attggcaacg
    18901 ccaataatct ctttttcgtc cgggtcgaat aagccaaagt agaaagctga accttgttta
    18961 tgaaattcgt taatcatccc cagcctggcc tgccagcctg atggataaca gtggctttcg
    19021 tcgcgcactg gctcccaggg cttgaggaaa tggcgattct ctgcgtaata atccgcaaga
    19081 cgccaggcat cacgatcatg caccagacgc acgaccagtc ggtctgtggt taagcgcact
    19141 tttggcacgt tactgcgata gccaaacatt ctatacctac tccttcccgt aacgtcttta
    19201 atacgctgta agtattacta tacctgcgca accagaccct gtgaaaagtc caaaatgcca
    19261 ttttctgcat tattcacata tttgataaaa actctctatc aaagcgacgt tttgataaaa
    19321 aaatattgtc cccgaagtga tgggaaaagt cttagcggca atgcagaata gaaagtgctc
    19381 atcttttatt tcccgggagg ggaaatgtcc cgcgtatcgc aggcgaggaa cctgggtaaa
    19441 tatttcctgc tcatcgataa tatgctggtc gtgctggggt tctttgttgt cttcccgctg
    19501 atctctatcc gcttcgttga tcaaatgggc tgggccgccg tcatggtcgg tattgctctc
    19561 ggtctacgcc agtttattca gcaaggtctg ggtattttcg gcggcgcaat tgccgaccgc
    19621 tttggtgcca aaccgatgat tgttaccggt atgctgatgc gcgccgccgg attcgccaca
    19681 atgggtatcg cccacgaacc gtggctattg tggttttcat gcctgctctc gggactcggc
    19741 ggcacgttgt ttgatccgcc gcgttcggcg ctggtggtga aactaatccg tccacaacaa
    19801 cgtggtcgtt ttttctcgct gttgatgatg caggacagtg ccggtgcggt cattggcgca
    19861 ttgttgggga gctggctgtt gcaatacgac tttcgcctgg tctgcgccac aggggccgtt
    19921 ttgtttgtgc tgtgtgcggc gttcaatgcg tggttgttac cggcatggaa actctccacc
    19981 gtacgcacgc ccgttcgcga aggcatgacc cgcgtgatgc gtgacaagcg ttttgtcacc
    20041 tatgtcctga cgctggcggg ttactacatg ctggctgtac aagtgatgct gatgctgcca
    20101 attatggtca acgacgtggc tggcgcgccc tctgccgtta aatggatgta tgccattgaa
    20161 gcgtgtctgt cgttaacgtt gctctaccct atcgcccgct ggagtgaaaa gcattttcgt
    20221 ctggaacacc ggttgatggc tgggctgttg ataatgtcat taagcatgat gccggtgggc
    20281 atggtcagcg gcctgcaaca acttttcacc ctgatttgtc tgttttatat cgggtcgatc
    20341 attgccgagc ctgcgcgtga aaccttaagt gcttcgctgg cggacgcaag agctcgcggc
    20401 agctatatgg ggtttagccg tctgggtctg gcgattggcg gcgctattgg ttatatcggt
    20461 ggtggctggc tgtttgacct gggcaaatcg gcgcaccagc cagagcttcc gtggatgatg
    20521 ctgggaatta ttggcatctt cactttcctt gcgctgggtt ggcagtttag tcagaaacgc
    20581 gccgcgcgtc gtttgctgga acgcgacgcc tgatttgata ctcccccctg actcctccat
    20641 actgaaagtg aactgacagc caaatggagg agtcacgtga agctatacat ttacgatcac
    20701 tgcccttact gcctcaaagc ccgcatgatt ttcggcctga aaaatatccc cgtcgaatta
    20761 catgttctgc tcaacgacga cgcagaaaca cccacccgga tggtcggtca aaaacaggtt
    20821 cccattctgc aaaaagatga cagccgctat atgccagaaa gcatggatat cgttcactat
    20881 gtcgataaac tcgacggcaa accgttactg accggcaaac gttcccctgc cattgaagag
    20941 tggctgcgca aggtcaatgg ctacgccaac aaactgctgt tgccgcgttt tgccaaatcg
    21001 gcatttgatg agttttctac tcccgccgcg cgcaaatatt tcgtcgacaa gaaagaggcc
    21061 agcgcgggta attttgccga cctgctggcc cactctgacg gtctgattaa gaatatcagc
    21121 gatgatttgc gtgcgctgga caaactgatc gtcaaaccga acgccgtgaa tggagaactt
    21181 tcggaagatg atattcagct atttccgcta ctgcgtaatc tgacgctggt agccggaatc
    21241 aactggccaa gccgcgttgc tgattaccgc gataatatgg cgaaacagac acaaatcaat
    21301 ttgttatcat caatggcgat ttaatcctga cccgccgcgg cagcctgtat tcgcgggttg
    21361 ccgccgtttt cagttttttc tgctggcgag tttatcgctg gtgacacatg ctgcgttgtg
    21421 tcaggatatt gacgcagatt tcatgtcgag gactaccatg aacaagtttt tatttgccgc
    21481 tgcattgatc gtcagtggcc tgctcgttgg ctgtaatcaa ctcacccaat acaccatcac
    21541 cgaacaagaa attaaccagt cgcttgcgaa acataataat ttctcaaaag atatcggttt
    21601 acccggcgtg gctgacgccc atattgttct gacaaacttg accagccaaa ttggtcgcga
    21661 agagccgaat aaggttaccc taaccggaga cgccaatctg gacatgaact ccctgttcgg
    21721 tagtcagaaa gcgaccatga aactgaagct gaaagcgctg cccgtgtttg ataaagaaaa
    21781 aggtgcgatc ttcctgaaag agatggaagt ggtcgatgcg acggtacaac cggaaaaaat
    21841 gcaaacggtg atgcaaacgt tgcttcccta tttgaaccag gcattacgca attactttaa
    21901 ccagcaacct gcttacgtcc tgcgcgaaga tggcagtcag ggcgaagcaa tggcgaaaaa
    21961 actggcgaaa ggcattgaag tgaagccagg cgaaattgtc attccgttta cggattaatg
    22021 acgagggcgc attcgcgccc tttatttttc gtgcaaagga aaacgtttcc gcttatcctt
    22081 tgtgtccggc aaaaacatcc cttcagccgg agcatagaga ttaatgactg caccatccca
    22141 ggtattaaag atccgccgcc cagacgactg gcaccttcac ctccgcgatg gcgacatgtt
    22201 aaaaactgtc gtgccgtata ccagcgaaat ttatggacgg gctattgtaa tgcccaatct
    22261 ggctccgccc gtgaccactg ttgaggctgc cgtggcgtat cgccagcgta ttcttgatgc
    22321 cgtacctgcc gggcacaatt tcaccccatt gatgacctgt tatttaacag attcgctgga
    22381 tcctaatgag ctggagcgcg gatttaacga aggcgtgttc accgctgcaa aactttatcc
    22441 ggcaaacgca accactaact ccagccacgg cgtgacgtca attgacgcaa tcatgccggt
    22501 acttgagcgc atggaaaaaa tcggtatgcc gctactggtg catggtgaag tgacacatgc
    22561 agatatcgac atttttgatc gtgaagcgca ctttatagaa agcgtgatgg aacctctgcg
    22621 ccagcgcctg actgcgctga aagtcgtttt tgagcacatc accaccaaag atgctgccga
    22681 ctatgtccgt gacggaaatg aacggctggc tgccaccatc actccgcagc atctgatgtt
    22741 taaccgcaac catatgctgg ttggtggcgt gcgtccgcac ctgtattgtt tacccatcct
    22801 caaacgcaat attcaccaac aggcattgcg tgaactggtc gccagcggtt ttaatcgagt
    22861 attcctcggt acggattctg cgccacatgc acgtcatcgc aaagagagca gttgcggctg
    22921 cgcgggctgc ttcaacgccc caaccgcgct gggcagttac gctaccgtct ttgaagagat
    22981 gaatgctctg cagcactttg aagcattctg ttctgtaaac ggcccgcagt tctatggctt
    23041 gccggtcaac gacacattca tcgaactggt acgtgaagag caacaggttg ctgaaagcat
    23101 cgcactgact gatgacaccc tggtgccatt cctcgccggg gaaacggtac gctggtccgt
    23161 taaacaataa aaatcgtagc ttcctgttgt cattaggtta ttttacctgt ataaataacc
    23221 agtatattca acagggggct attatgcgaa ttgaagtcac catagcgaaa acttctccat
    23281 tgccagctgg ggctattgac gccctggctg gcgaactttc ccgccgtatt cagtatgcgt
    23341 ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcgaataat ttatcggtta
    23401 ttggcgtaac aaaagaagat aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa
    23461 gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt gccgggtcgc
    23521 cccggctttt ttattttact tacaaatttt gagtacaaaa tttctacact tcctcaaaat
    23581 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
    23641 taaaagcaga aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
    23701 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
    23761 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
    23821 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
    23881 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
    23941 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
    24001 gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
    24061 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
    24121 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
    24181 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
    24241 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
    24301 catgcttatg gtgaaggcga aaagtatgtc ccgttggtcc tccgcgcgca aacgctgtgg
    24361 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
    24421 ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa
    24481 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc
    24541 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
    24601 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg
    24661 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaaccgttga cggtgagtat
    24721 caggcgaaaa aagcgattgt ctgcacggga acatgggtaa aagacctgct cccggagctg
    24781 cctgtccagc ctgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
    24841 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
    24901 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
    24961 gcggatgaac gtgttccgtt tgcggaagtg gtcagcgatg gttcggaagc cttcccgttc
    25021 ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
    25081 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
    25141 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
    25201 gattttgcgc aagacaaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
    25261 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
    25321 ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
    25381 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
    25441 cataaactga caaatattta atctcccttt catttagcgt gaaaaattga atgaaattat
    25501 tcctgcttct acagcactca ccagacatct ctgatgctga acccactaaa cttatgaaga
    25561 ttctttcatt attaattcaa aattattgaa ataaaaataa atatcattca atttgttatt
    25621 tttgtgttgc aattcattct ccgacacgcc attttctttc tctcgcaaat tcgtattgga
    25681 tgcacttatg tcattcacaa atacccctga acgctatggt gttatatctg cggccttcca
    25741 ttggctaagc gcaattattg tctacggcat gtttacttta ggtctgtgga tggtcacgct
    25801 cagttattac gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt
    25861 gttaatgatg gggctggtta ttcgcgttct gtggcgtgtc atatctccac cgccgggtcc
    25921 gctgccgagt tattcgccca tgactcgtct tgccgcaaag gctggacatc tcgcattgta
    25981 cctattgctg ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc
    26041 gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc
    26101 agactttgcc ggtgccctgc atttttggct cgcgtggagc gtcgtagtac tgtccgttat
    26161 gcacggattt atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat
    26221 gctgggaaag tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt
    26281 aaccttcgcg tccctgatgt tctctgccgg ttcagcggtt gccgccgatt acaaaattga
    26341 caaagaaggt cagcacgcct ttgttaattt ccgcatccag caccttggct atagctggtt
    26401 atacggcacc tttaaagatt tcgacggtac ttttaccttt gacgaaaaaa atccggctgc
    26461 cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat actaatcacg ccgaacgcga
    26521 taaacatctt cgcagtgcag atttcctcaa taccgcaaaa tatccacagg caacattcac
    26581 ctccaccagc gtgaagaaag acggtgacga actggatatt accggcgatc tgacgctgaa
    26641 tggcgtaacc aaacccgtca cgctggaagc gaaattaatt ggtcagggca acgacccatg
    26701 gggtggtaaa cgtgcaggct tcgaggccga aggcaaaatt aagctcaagg acttcaatat
    26761 caagacagat ttaggtccag cttctcagga agtggatctg attatttcag tggaaggcgt
    26821 acagcagaag taagcgtgaa atgttgccgg atggcacgca tccggcaatg atatttattc
    26881 tgttggatca ggaatgccca gtgttgtatt cagacgtcca cgagacttat taaagatctt
    26941 attgccattt tcacgtcctg cccgacggcg tcgctgttct tctggcggta acgcgctttc
    27001 ttcgcagcaa atctcactac aacaaccttt gtatttttcc gcgcatactg gacactgaat
    27061 aaacagcagg tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcgccgca
    27121 ctggtggcaa tgcgcgataa tctcatccga tatacgttcg cccatccgct cgtcaaaaac
    27181 aaaatttttg ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc
    27241 aataattccg ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaagcact
    27301 ggccttttca caacgaatgc cgccggtgca gtacatgacg atttttttat cttta
//
LOCUS       DAJVOY010000039        26833 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVOY010000039 DAJVOY010000000
VERSION     DAJVOY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.039"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..975)
                     /gene="eno"
                     /locus_tag="OQL90_001458"
     CDS             complement(<1..975)
                     /gene="eno"
                     /locus_tag="OQL90_001458"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012005243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1368280.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTK"
     gene            complement(1063..2700)
                     /gene="pyrG"
                     /locus_tag="OQL90_001459"
     CDS             complement(1063..2700)
                     /gene="pyrG"
                     /locus_tag="OQL90_001459"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1368281.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(2928..3719)
                     /gene="mazG"
                     /locus_tag="OQL90_001460"
     CDS             complement(2928..3719)
                     /gene="mazG"
                     /locus_tag="OQL90_001460"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1368282.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEESYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(3790..4125)
                     /gene="mazF"
                     /locus_tag="OQL90_001461"
     CDS             complement(3790..4125)
                     /gene="mazF"
                     /locus_tag="OQL90_001461"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCS1368283.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4125..4373)
                     /gene="mazE"
                     /locus_tag="OQL90_001462"
     CDS             complement(4125..4373)
                     /gene="mazE"
                     /locus_tag="OQL90_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCS1368284.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4451..6685)
                     /locus_tag="OQL90_001463"
     CDS             complement(4451..6685)
                     /locus_tag="OQL90_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1368285.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(6733..8034)
                     /gene="rlmD"
                     /locus_tag="OQL90_001464"
     CDS             complement(6733..8034)
                     /gene="rlmD"
                     /locus_tag="OQL90_001464"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1368286.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8091..10847
                     /gene="barA"
                     /locus_tag="OQL90_001465"
     CDS             8091..10847
                     /gene="barA"
                     /locus_tag="OQL90_001465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1368287.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11079..12419)
                     /gene="gudD"
                     /locus_tag="OQL90_001466"
     CDS             complement(11079..12419)
                     /gene="gudD"
                     /locus_tag="OQL90_001466"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1368288.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12440..13780)
                     /locus_tag="OQL90_001467"
     CDS             complement(12440..13780)
                     /locus_tag="OQL90_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1368289.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(13782..15152)
                     /gene="gudP"
                     /locus_tag="OQL90_001468"
     CDS             complement(13782..15152)
                     /gene="gudP"
                     /locus_tag="OQL90_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1368290.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15569..16018)
                     /locus_tag="OQL90_001469"
     CDS             complement(15569..16018)
                     /locus_tag="OQL90_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1368291.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16036..16818)
                     /gene="truC"
                     /locus_tag="OQL90_001470"
     CDS             complement(16036..16818)
                     /gene="truC"
                     /locus_tag="OQL90_001470"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1368292.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(16818..17147)
                     /locus_tag="OQL90_001471"
     CDS             complement(16818..17147)
                     /locus_tag="OQL90_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1368293.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(17769..18314)
                     /gene="syd"
                     /locus_tag="OQL90_001472"
     CDS             complement(17769..18314)
                     /gene="syd"
                     /locus_tag="OQL90_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1368294.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18382..19230
                     /gene="queF"
                     /locus_tag="OQL90_001473"
     CDS             18382..19230
                     /gene="queF"
                     /locus_tag="OQL90_001473"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1368295.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPKVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19342..20706
                     /gene="ppnN"
                     /locus_tag="OQL90_001474"
                     /gene_synonym="ygdH"
     CDS             19342..20706
                     /gene="ppnN"
                     /locus_tag="OQL90_001474"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1368296.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKAVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21263..22552
                     /gene="sdaC"
                     /locus_tag="OQL90_001475"
     CDS             21263..22552
                     /gene="sdaC"
                     /locus_tag="OQL90_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1368297.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22610..23977
                     /gene="sdaB"
                     /locus_tag="OQL90_001476"
     CDS             22610..23977
                     /gene="sdaB"
                     /locus_tag="OQL90_001476"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1368298.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24089..24844
                     /gene="xni"
                     /locus_tag="OQL90_001477"
     CDS             24089..24844
                     /gene="xni"
                     /locus_tag="OQL90_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1368299.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(24949..26086)
                     /gene="fucO"
                     /locus_tag="OQL90_001478"
                     /pseudo
     CDS             complement(24949..26086)
                     /gene="fucO"
                     /locus_tag="OQL90_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26114..26761)
                     /gene="fucA"
                     /locus_tag="OQL90_001479"
     CDS             complement(26114..26761)
                     /gene="fucA"
                     /locus_tag="OQL90_001479"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1368300.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6492 a         7209 c         6465 g         6667 t
ORIGIN      
        1 cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc ccagaacttt
       61 ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga tagaaacgat
      121 cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg tgaacgcttt
      181 gttgccttcg ccagccagaa cgtatttacc atctttgtag aattcagaag ctgcgcagtc
      241 catcgccaga gtgatgtctt tgcccagttc ataaccagca gctttaacag cttcagcgat
      301 aacagccaga gcttcagcgt tggaacccag gttcggcgca tagccacctt cgtcaccaac
      361 agcagtgttc atgcctttcg ctttcagaac ttttgccagg tgatggaaaa cttcagaacc
      421 catgcggatg gcttctttca cagttttcgc gccaaccggc tgaatcatga attcctggat
      481 atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg gaaccggcat
      541 agagtatttg cccggagtac cgttcagttc agcgatgtgc tcgtacagcg gcataccttt
      601 agcagctgca gcagctttgg cgttagccag agatacagcc aggattgcgt tcgcgccgaa
      661 tttggatttg ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc cagcctgatc
      721 tttagcatct ttgccaatca gcgcctgagc gatcgggccg tttaccgcag caacagcttt
      781 ggttacgcct ttacccagga aacgggattt gtcgccatcg cgcagttcca gagcttcacg
      841 ggaaccagta gaagcacctg acggagcagc tgccataccg acgaaaccac cctccagatg
      901 tacttcggct tcaacagtcg ggttaccacg ggagtcgatg atttcacgac cgatgatttt
      961 tacgattttg gacattaggt tttcctcaag tcactagtta aactgaaact ccagacaaac
     1021 aacacgtacc cagggtacgc gttgccgttc taactttttt acttacttcg cctgacgttt
     1081 ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat cacgtggagt
     1141 agaagtaaac tccggatgga actggcaagc cacgaaccac gggtgattcg gaacttcgat
     1201 gatctcgacc aactgatcat ccccggaacg gcccgcaacg cgcagacctg catcttcaat
     1261 ctgtttcaac agcatgttgt tgacttcgta acggtgacga tgacgctcaa caattgtcgg
     1321 cgcattgtac agctggcgaa ccaggctatc gtcaaccaac tggcactgct gtgcgccgag
     1381 acgcatggta ccgccgagat cgctcttctc gctacgaact tcaacgttgc cgttttcatc
     1441 gcgccactcg gtaatcagcg ccacaaccgg gtacttacag tctggcacaa attccgtaga
     1501 gttggcgttc tccatgttgg caacatggcg agcgtaatca attaacgcca cctgcatacc
     1561 caggcaaatg cccagataag gaatattgtt ctcacgcgca aaacgcgcgg tcgtaatcat
     1621 gccttctacg ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca gacctttaag
     1681 gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt tgacgctgac
     1741 acgattcttc agcccaccgt gtttcagtgc ttcgatcact gatttataag catccggcag
     1801 ttcaatgtac ttgccgacca taccgatggt gacttcactt accgggttcg cttcttcgaa
     1861 gataacctgt tcccattcgg acagattcgc ttccgggcag tttaagctga atcgtttaca
     1921 aatataatcg tccagcccct gagatttcaa caggcccgga attttataga tggaatcgac
     1981 gtctttcaga gaaataaccg ctttttccgg aacattacag aacaatgcaa tcttcgcacg
     2041 ttcgttcgcc ggaacagcgc gatctgaacg acaaatcagg atgtcaggct ggataccgat
     2101 ggagagcagc tcttttacag agtgctgagt cggtttggtt ttgacttcac cagacgctgc
     2161 catgtacggc accagcgtca ggtgcataaa cagagtgtgc tcacggccaa tttcaacagc
     2221 catctggcga atcgcttcga ggaacggcaa ggattcgata tcacctactg taccgccgat
     2281 ttctaccagt actacgtcat gaccttcgcc accttccagc acgcgctctt tgattgcgtt
     2341 agtgatgtgc ggaataacct gcacggttgc gccgaggtag tcaccgcggc gttctttacg
     2401 cagaacgtca gagtagatac gacccgtggt gaagttgttg cggcggctca ttttggtacg
     2461 aatgaaacgc tcgtagtgcc ccaggtccag gtcggtttca gcgccgtctt cagtaacgaa
     2521 cacttccccg tgttggattg ggctcatagt acctggatcg acgttgatgt acggatccag
     2581 tttcatgatg gtcacattga ggccacgggc ttcaagaatg gctgcgaggg aggctgcggc
     2641 aatgccttta cccagagagg atacgacccc gccggtcaca aaaatatagt tcgttgtcat
     2701 gctgaacctg agaagttagg ttgaaaagac gatggaataa ccaggacggg aaagcagtat
     2761 acccgaacat gacctgtgcc acaaactttc attatccctc ctcttcgcca gcgcactatt
     2821 gaaatcagga gtgagaaaat agcccctttg gggtaaatgt ttttgacgca aatcaagcgc
     2881 ttgtcattta aaaaatgaca caaatggcac ttgaccgcgt aattccctta gagatcaatt
     2941 tcctgccgtt ttacctgttg ccagacttct tccattgttt cgaggtcaac acctgtcatt
     3001 tccagtccac gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc gaatttttcg
     3061 ttcgcttttt gcaatgcgat ttctgctttc gtccctaaat ggcgagccag attaaccgtg
     3121 gcaaacagca ggtcccccat ttcctcctcc agtttagcct ggtcgacaac agcctgccgc
     3181 gcttcgtaca tcacctcgtc gatctcttcg tagactttat caaccaccgg gccaagcgtc
     3241 gtccagtcga agccaacgtt ggcgcaacgt ttctggattt tttgcgcccg cattaaagcc
     3301 ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc gcgctcttcg
     3361 gttttgattt gctcccaacg ggcaagcact tcactactgt tttcggcaga actatcagca
     3421 aaaacatgcg gatggcgacg ctctaattta tcgctaatag cagcgcaaat atcattaaag
     3481 tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca ccacctggaa tagcagatcg
     3541 cccagttcac cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag cacttcgtag
     3601 ctttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc ccacgggcag
     3661 ccgttttccg gatcgcgcag gcgctgcata atagtgagca aacgatcgat ttgattcatt
     3721 gaattgtcct gaaaattgcg ggtctgtcag gtggaaacct gtgaccagaa tagaagtgag
     3781 ttagtaacac tacccaatca gtacgttaat tttggcttta atgagttgta attcctctgg
     3841 ggcaactgtt cctttcttcg ttgctcctct tgcccgccag gcgattcttt ttacctgatc
     3901 agctaacgct acgccatcac gttcctgacc ggataaaaca acttcgaacg gatatccttt
     3961 tgattgcgtt gtacaaggaa cacacagaca catacctgtt ttgttgttgt acatgaaagg
     4021 actcaggaca acagctggac gatgtccagc ttgctcgcta ccttttgtcg ggtcaaaatc
     4081 aacccaaatc agatcgccca tatcgggtac gtatcggctt accattacca gacttcctta
     4141 tctttcggct ctccccagtc gatattctcg tggaggtttt ccggcgtgat gtcgttgacc
     4201 agttcagcaa gcgtaaatac gggctcttta cgcactggct caataattaa tttgccatcc
     4261 accaggtcaa tcttcacttc atcatcaata ttcagattga gcgcctgcat taacgtagcc
     4321 gggatccgca ccgccggtga atttccccaa cgctttacgc tactgtggat catcaccctt
     4381 tcctcaaacc gctatcatat gtagatacag tatatatcaa tctacattgt agatacgagc
     4441 aaatttctgc ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     4501 gtttacccag cacgcggccc agcacctgca ggttgtaaat ctcaatggtc atgtcgatgg
     4561 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagtacgttc accttctcgt
     4621 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     4681 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     4741 gcgcatgaga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     4801 gcccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     4861 tgtggtgcat caggttgcca acaccttcga ctaccacgcg accgttatct ttactgcggt
     4921 tttgcggcgt gtagcttttt tgctgaagtt gcttcagcgc ggcggcgtcc tgctcttcgg
     4981 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5041 cgccaccaat cgccgccagc aactcgtcga catcattaaa gttgtaacgc ggcagcagat
     5101 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5161 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5221 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5281 gctgtttctg ggtgataatt tcaatctggt cgcccatctg cagctggtag gtgaacggca
     5341 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5401 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     5461 agacatacac ccggtcgtca aagacctggc tgcgtacttc gtcgagcatt tcgccggaat
     5521 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     5581 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     5641 actctgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt ttgccacctg
     5701 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacataat
     5761 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     5821 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     5881 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cggccataca
     5941 cttcagcttt aacgccttca gctttcatct cagcgcgcag atgaccgaca aactcttcga
     6001 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6061 tcgggtgcag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6121 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acgcgttcat
     6181 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacca
     6241 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6301 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6361 cgtgaataag gttaacgaca gacttaccga cgctctcacg cagcacatct tcgctgacta
     6421 cgttggcatc ggccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     6481 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     6541 gcccctgcgt ctgctgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     6601 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     6661 tatgtgcact tcttaccgca accattgtcc tctcctttag ggaccagacc tgccgaaatc
     6721 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     6781 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     6841 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     6901 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     6961 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7021 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7081 cggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7141 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7201 cgcactcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7261 aaaagttaag cgcaacccgt tagagtcata ccagggcatc tcaccagaga cggtttcgag
     7321 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7381 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7441 ggttgcctgt accagttcaa catgaccaag atggcgcatt gcttgtaagc tgcccaggca
     7501 tgccctgact ttgggcagca atgcttcaag ttggggcact aaaatgggac attgtttaac
     7561 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     7621 taagtagttc agacttaaac gcgcgcgacg gcgatagccc cagggaacat cggcgatcac
     7681 ttcagagact tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     7741 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     7801 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     7861 tttatcttca gtaacagtaa cttccgcgtt ttcctgcggc aataatccgg ggataaatag
     7921 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     7981 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8041 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8101 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8161 tgctgagtat atttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8221 caggtgccag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8281 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8341 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8401 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     8461 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     8521 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     8581 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc agcgtgatga
     8641 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     8701 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     8761 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     8821 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     8881 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     8941 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9001 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9061 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9121 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9181 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9241 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9301 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9361 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9421 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
     9481 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
     9541 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
     9601 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
     9661 ttattatcga agggccatcc acccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
     9721 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
     9781 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
     9841 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
     9901 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac
     9961 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctaca cgcctgttgc
    10021 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10081 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10141 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcgg
    10201 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10261 tggatggcat tcgggcctgc gaactcatcc accagctccc ccatcagcaa caaacgccgg
    10321 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10381 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10441 acaagcctgg cagcggtatt tcctctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    10501 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    10561 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    10621 ttgaggaaca gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    10681 atggcagttg cggctatagc ggtgtgccgc gtatgaagaa tctctgccaa ctgatcgaac
    10741 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    10801 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    10861 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    10921 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    10981 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11041 aagccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11101 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatt gtcacgcgcg
    11161 caaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11221 acacccagcc ccggtttttc cggcacctga accagaccgc ctttgatctc aaacggttct
    11281 ttggtcaagc gctgattgcc ttcctgccaa atccagtgcg tatcaatcgc ggtgatttta
    11341 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11401 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    11461 tgcatcgtcc agaaatgcgg atccgccagc gggatatcaa cggattgcag ggagagcgta
    11521 tggcccatct gccgccagtc ggtggcgatc atattggttg cggtcggcag gccagttgcg
    11581 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    11641 tctgcataag ccagcgaccc tttcaggtat ttaccaattt taatcgcttc gttcagcgac
    11701 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    11761 atagactcgg cctcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    11821 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    11881 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagcggt
    11941 gtggctttgc gatcaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12001 tgaccatcgc ccagcaggga tgccacgttt acccccagat gctgccccag cagatccagc
    12061 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12121 ccgcgcccac cagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12181 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12241 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccag aattatcttt gataatcata
    12301 atattacgcg taaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12361 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12421 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    12481 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcca ggcagacgtt
    12541 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    12601 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    12661 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    12721 ccacatgggt aaacatcgcc agagagatat cgaaatggtt attagaatgg cagccccagg
    12781 tcagccccca gtcgtcgcaa agctgcgcca cacggactgc accggaaagc gtccagaagt
    12841 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    12901 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgccga aactccgcca
    12961 taacttcacg accggagaaa ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13021 catcattcag ccctttgcat agagaaatgg cttcatcaag tagccaggca ccgttgggat
    13081 caacggtaat ccgcgcatca gggaagcgtt tcttcaatgc acgaacagtg tcgatttctt
    13141 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13201 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13261 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13321 cgatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13381 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13441 ccgccacggc attaacgtgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    13501 cggctgcctg attaccttta tgcacctgct gaaccacttt attcaggcgc gcaacttcct
    13561 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    13621 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacaatattg cgggtgaaat
    13681 atgcgttatg tgccccacca atattaagca acatgctatc gtgcccggcg accggaatga
    13741 ctttcatatc agtaataaca ggactggatt gtgtcgtcat cttattgccc cgcgacaggt
    13801 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattaag
    13861 gcatgaacac caacataaat cagcgcccca ttaaaggagc cagtcgtgcc aacgatataa
    13921 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    13981 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14041 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14101 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14161 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataatccc acccagcaca
    14221 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaattgac
    14281 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14341 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagttgtttg
    14401 atctgccccc acttcacgct gaacggcact ttaactttgg tgttttgctg atccatattg
    14461 atcagcgcgc cacccgcggc gatgtactcc agctctttct gatttacccc cggatgttga
    14521 tttggctcgt ggatgacttt caaccagata aagctgatga caatccccag accgcccata
    14581 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    14641 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    14701 gccggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    14761 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    14821 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    14881 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    14941 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15001 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15061 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15121 cttaaagaac tcgttgttgc actcctgaaa attcgcgtta gccacgctaa ctctggactg
    15181 cgacatagcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15241 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15301 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15361 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggttaaaac
    15421 gaagaaataa agaagagtat gaaatggatc gcttgactcc aggcaaacgc cagtaaaaat
    15481 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    15541 aaacgacatt taccgctcgc tgaacatatc aggacaacag cgtgccccac tgttcgaccc
    15601 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    15661 caactcgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaga
    15721 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    15781 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    15841 cggtcgtgga cgtaaccacc agaacatact tatcctgata aggcagccag tcgcttaatt
    15901 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    15961 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16021 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16081 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16141 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16201 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16261 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16321 tcggcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16381 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16441 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    16501 ttccaccagt ggataatcca gcaccgcttc ttccatcaac cagccgcgca caatcgcatg
    16561 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    16621 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    16681 atgctgtcct atctggttac gcacggtttg catgaccact actttctcgt cgcgatccag
    16741 ccagctacgg tgaaccagcc agccggaggg tttatttacc gaaaccagcc attcatcctg
    16801 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    16861 tcagccagaa taagcgcgtg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    16921 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    16981 atcagcaccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17041 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17101 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17161 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagcg
    17221 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17281 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17341 ccccgatgtg atacttcatc ctgaagtgtc cttggccttc ctgaccagcc gaatcatcct
    17401 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    17461 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    17521 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    17581 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    17641 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    17701 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    17761 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    17821 tgcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    17881 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    17941 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18001 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18061 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18121 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18181 gtagttgaaa taatgcacgg tgaaggaaca ccgtacagtt cctcacttaa cggccagctt
    18241 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18301 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18361 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18421 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    18481 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    18541 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgaaggtc
    18601 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    18661 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    18721 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    18781 cttgatgaac tggaaggcca gccgataggt cattttaatg gcacttgcat tgatgaccag
    18841 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc cacctgtggt
    18901 gaaaaagtag tggaagagac gcttgtcagc cacctgctga aatcaaactg cctgatcacc
    18961 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19021 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19081 gaacgcatct ttaatgacct gttacgcttc tgccagccag aaaaattgag cgtttacgca
    19141 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19201 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19261 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19321 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19381 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19441 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    19501 ttgctgtctc gttttgaaaa tttcgatatt aacgtcttgc gccgtgaacg cggcgtaaag
    19561 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    19621 caggccaact tgttcgcggt cctgcgtgac attctcttcg tttacgggca aatccataac
    19681 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    19741 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    19801 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cggaaaccag
    19861 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc cgggagcgat ggaagcgccg
    19921 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    19981 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cgctggtcaa cgaattgatc
    20041 atcatgccag atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggtatcatt
    20101 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20161 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20221 gactacttcc gcgtactgga cgagtttgtc gtgcatacgc tgggtgaaaa cgcgcgccgc
    20281 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20341 ccgctggtga aagaaaatcg ccgcgacaca ggcgatgcct acagctttaa ctggtcaatg
    20401 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    20461 aagctttacc cggatcaacc cgttgaagtg ctagctgccg acctgcgccg tgcgttctcc
    20521 ggtattgtgg cgggtaacgt aaaagcagtc ggtattcgcg ccattgaaga gtttggtcct
    20581 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    20641 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    20701 acgtaatctc ccctcccccg gtgacgtttg ttgccgggtt ttctcgtttt tggtcactta
    20761 ctcatcaact catttcattt gttatatgaa tgtttcttac caccctcacg cgacaaatat
    20821 catcacagtt aatatgtcat acaatttatg ttgcaacgca aacgtttctc tattttcata
    20881 aaccgtattt tatcgctatg aaaaagaaat tatcgccatg attaactaaa agtattgatt
    20941 ttttcagttc aacctacata tattgcgcgc cctggaagaa gtcagatgtc gtttaatggg
    21001 caaatattgc ccttaaattc tcttttactt ttgatttaca gagtaaagcg ttgggataat
    21061 ctatcttcca agtagattat tgtatttgag atcaagatca ctgatagata cataacttgt
    21121 gtgtatcttt ccgccctcaa attattacgg cggtaaatga ttaagccatc gccgatagac
    21181 agatttcatt tttacggtca ggcaccttcc cgggctgaac tggctaaaag ctgaattatt
    21241 tgcattcctc caggagaaat agatggaaac gactcaaacc agcacgattg cgtcgaaaga
    21301 ctctcgtagt gcctggcgca agacagacac catgtggatg ctgggccttt acggcacggc
    21361 aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt gttggcggta tgatcccgct
    21421 gatcatcatg gctatccttg cgttcccgat gacgtttttt gctcaccgcg gcctgactcg
    21481 cttcgtactg tctggtaaaa acccgggcga agacatcacc gaggttgtag aagaacactt
    21541 tggtattggc gcaggtaaac tgattaccct gctctacttc ttcgctatct acccgatcct
    21601 gctggtttat agcgtggcaa tcaccaatac agttgaaagc ttcatgtctc accagctggg
    21661 tatgacgcca ccgccgcgtg cgattctgtc gctgatcctg atcgtgggta tgatgaccat
    21721 cgttcgcttc ggtgagcaga tgatcgttaa agcgatgagt attctggtgt tcccgtttgt
    21781 tggcgtattg atgctgctgg ctctgtacct gatcccgcag tggaacggcg cagcactgga
    21841 aacgctgtct ctggacactg catctgcaac cggaaacggt ctgtggatga ccctgtggct
    21901 ggcaattccg gtaatggtgt tctcgttcaa ccactctccg atcatctctt ctttcgccgt
    21961 tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa tgctcgaaga tcctggcatt
    22021 cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc ttcagctgtg tactgagcct
    22081 gactccggca gacctggctg cggctaaaga gcagaacatc tcgattctgt cttacctggc
    22141 taaccacttt aacgcaccgg ttatcgcgtg gatggctccg attatcgcga ttatcgctat
    22201 caccaaatcc ttcctcggcc actacctggg cgcacgtgaa ggcttcaacg gtatggtgat
    22261 taaatctctg cgtggtaaag gtaagtctat cgaaatcaac aagctgaacc gtatcactgc
    22321 gctgttcatg ctggtaacga cctggattgt tgccaccctg aacccgagca tcctgggtat
    22381 gattgaaacc ctgggcggtc caatcatcgc gatgatcctg ttcctgatgc cgatgtacgc
    22441 aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac atcagcaacg tattcgttgt
    22501 cgtgatgggt ctgattgcaa tctccgcaat cttctactct ctgttcagct aagtcctttc
    22561 gcgccgcttt cgggcggcgc ttcctccgtt ttaacgcgat gtatttccta tgattagcgt
    22621 attcgatatt ttcaaaatcg gcattggccc ttccagttct cataccgttg gaccaatgaa
    22681 agcgggtaaa caatttaccg acgatctgat tgcccgtaac ctgcttaaag acgtgacccg
    22741 cgtggtggtt gacgtgtacg gctcgctctc tctgaccggt aaaggccacc acactgatat
    22801 cgccattatt atgggcctgg cgggtaacct gccggatacc gtggatatcg attccatccc
    22861 tggttttatt caggatgtga atactcatgg tcgcctgatg ctggcaaacg gtcagcatga
    22921 agtggagttc ccggttgatc agtgcatgaa cttccacgcc gacaaccttt ctctgcatga
    22981 aaacggtatg cgcattaccg cgctggcggg cgataaagtc gtttacagcc agacttacta
    23041 ctctattggc ggtggcttta tcgttgatga agagcatttt ggccagcagg atagcgcacc
    23101 ggttgaagtt ccttatccgt acagttcagc agccgatctg caaaaacatt gtcaggaaac
    23161 cgggctgtca ctctctggcc tgatgatgaa aaacgagcta gcgctgcaca gcaaagaaga
    23221 gctggaacag cacctggcga acgtatggga agtcatgcgt ggcggtattg agcgcggtat
    23281 ttccaccgaa ggcgtgttgc ctggcaaact gcgcgttcca cgccgtgctg cggcactacg
    23341 ccggatgctg gtcagccagg ataaaaccac cactgacccg atggcggttg ttgactggat
    23401 caacatgttt gcactggcag tgaacgaaga gaacgctgct ggcggacgcg tggtgactgc
    23461 gccgactaac ggtgcgtgcg ggattatccc ggcagtgctg gcgtactacg acaagtttat
    23521 ccgcgaagtg aacgctaact cactggctcg ttacctgctg gtagccagtg ccattggttc
    23581 tctttataag atgaacgcgt caatttctgg tgctgaagtc ggctgccagg gtgaagttgg
    23641 tgtggcgtgc tcaatggcgg cggctggtct ggcagaactg ttaggtgcaa gcccggcgca
    23701 ggtgtgcatc gcggcggaaa tcgccatgga gcacaacctc ggtctgacgt gtgacccggt
    23761 cgccggacag gtccaggtgc catgcatcga gcgtaacgcc attgcggcag taaaagcggt
    23821 gaacgccgca cgtatggcgc tacgccgtac cagcgagccg cgcgtctgcc tcgataaagt
    23881 tatcgaaacc atgtacgaaa caggtaaaga tatgaacgcc aagtaccgcg aaacctctcg
    23941 cggcggcctg gcaatgaaga tcgttgcctg cgattaatcg ctctccagag gcctcgtttt
    24001 gcgaggcctc ttcccgattt ctcatccagc cgtagcctgt tccggcatcg aatgttaccc
    24061 ttatcgcctg atctttaagg gggttatcgt ggctgttcat ttgcttattg tcgatgcact
    24121 gaatcttatt cgtcgcattc atgccgttca gggatcgccc tgtgtcgaaa cctgccagca
    24181 tgcgctcgat cagctcatta tgcacagcca gccaacccac gcggtcgccg tttttgatga
    24241 tgaaaaccgc agtagcggct ggcgtcatca gcgtttacca gattacaaag cgggtcgacc
    24301 gccaatgccg gaagagttgc acgacgagat gcctgcatta cgcgccgcct ttgagcaacg
    24361 cggcgtcccg tgctggtcaa ccagcggcaa cgaagccgat gacttagccg ccacgctggc
    24421 ggtcaaagtg acacaggccg ggcatcaggc aacgattgtt tcgacagata aaggctactg
    24481 tcagttactt tcaccgacat tacgtattcg tgattacttc cagaaacgtt ggctggatgc
    24541 accatttatc gataaagaat ttggcgttca accgcagcag ttgcccgatt actggggact
    24601 tgcggggatc agcagttcaa aggtaccggg tgttgcggga atcggaccaa aaagcgccac
    24661 gcagctgctg gtcgagtttc agagtctgga agggatatat gagaatctgg atgcggttgc
    24721 cgaaaagtgg cgcaaaaaat tagaaaccca taaagagatg gcgtttctgt gccgcgatat
    24781 tgcccgctta caaaccgatt tgcatatcga cggcaattta cagcaattgc ggttggtacg
    24841 gtaacggcga gccggatgca aacgattatc aacacattga atttaatgca cttatatacc
    24901 ggataagacg cggctagcgt cgtatccggc attatcacat cagcgcattt accaggcggt
    24961 atggtaaagc tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac
    25021 atcatccagt gccgcctgcg ccagtgccgg aatgtcttcc ttgcgtacac caacatcacg
    25081 caaatgtggc ggaataccga catcacggtt gagagcaaac accgcttcaa cagcggcatt
    25141 acgcgcctct tccagccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
    25201 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
    25261 gtggagtgtt gtaaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
    25321 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
    25381 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
    25441 gtgcatcggt tagcgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
    25501 catcgacacc cgtcgcagct ttcagcgctg gaggcatacc atccatcatg tcagcgtcaa
    25561 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttctctt
    25621 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtaggaattg
    25681 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
    25741 caaactccgg gttgttgctg ataatgccaa tcgctttaca agtatcctgt ggagaaccac
    25801 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
    25861 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
    25921 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
    25981 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
    26041 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
    26101 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
    26161 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
    26221 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
    26281 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
    26341 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
    26401 taccacctgc cgccgcaatc atgtagtgaa tagcggggat cggtcggtta agaatggaaa
    26461 ctgctgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
    26521 aggccgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
    26581 taccatcaat aaaggcaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
    26641 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
    26701 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
    26761 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
    26821 ttagccgttt aat
//
LOCUS       DAJVOY010000040        26808 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVOY010000040 DAJVOY010000000
VERSION     DAJVOY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.040"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..328)
                     /gene="rpnB"
                     /locus_tag="OQL90_001480"
     CDS             complement(<1..328)
                     /gene="rpnB"
                     /locus_tag="OQL90_001480"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1368301.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDA"
     gene            complement(525..1298)
                     /gene="hisP"
                     /locus_tag="OQL90_001481"
     CDS             complement(525..1298)
                     /gene="hisP"
                     /locus_tag="OQL90_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1368302.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1306..2022)
                     /locus_tag="OQL90_001482"
     CDS             complement(1306..2022)
                     /locus_tag="OQL90_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1368303.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(2019..2705)
                     /gene="hisQ"
                     /locus_tag="OQL90_001483"
     CDS             complement(2019..2705)
                     /gene="hisQ"
                     /locus_tag="OQL90_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1368304.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2795..3577)
                     /gene="hisJ"
                     /locus_tag="OQL90_001484"
     CDS             complement(2795..3577)
                     /gene="hisJ"
                     /locus_tag="OQL90_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1368305.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3798..4580)
                     /gene="argT"
                     /locus_tag="OQL90_001485"
     CDS             complement(3798..4580)
                     /gene="argT"
                     /locus_tag="OQL90_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS1368306.1"
                     /translation="MKKSILALSLLVGLSVAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4846..5415)
                     /gene="ubiX"
                     /locus_tag="OQL90_001486"
     CDS             complement(4846..5415)
                     /gene="ubiX"
                     /locus_tag="OQL90_001486"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1368307.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAISLPEDLFARWQGA"
     gene            complement(5510..7027)
                     /gene="purF"
                     /locus_tag="OQL90_001487"
     CDS             complement(5510..7027)
                     /gene="purF"
                     /locus_tag="OQL90_001487"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1368308.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(7064..7552)
                     /gene="cvpA"
                     /locus_tag="OQL90_001488"
     CDS             complement(7064..7552)
                     /gene="cvpA"
                     /locus_tag="OQL90_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1368309.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7811..8473)
                     /gene="dedD"
                     /locus_tag="OQL90_001489"
     CDS             complement(7811..8473)
                     /gene="dedD"
                     /locus_tag="OQL90_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1368310.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8463..9731)
                     /gene="folC"
                     /locus_tag="OQL90_001490"
     CDS             complement(8463..9731)
                     /gene="folC"
                     /locus_tag="OQL90_001490"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1368311.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9801..10715)
                     /gene="accD"
                     /locus_tag="OQL90_001491"
     CDS             complement(9801..10715)
                     /gene="accD"
                     /locus_tag="OQL90_001491"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1368312.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGSIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10871..11530)
                     /locus_tag="OQL90_001492"
     CDS             complement(10871..11530)
                     /locus_tag="OQL90_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1368313.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11613..12425)
                     /gene="truA"
                     /locus_tag="OQL90_001493"
     CDS             complement(11613..12425)
                     /gene="truA"
                     /locus_tag="OQL90_001493"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1368314.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12425..13438)
                     /locus_tag="OQL90_001494"
     CDS             complement(12425..13438)
                     /locus_tag="OQL90_001494"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1368315.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13504..14640)
                     /gene="pdxB"
                     /locus_tag="OQL90_001495"
     CDS             complement(13504..14640)
                     /gene="pdxB"
                     /locus_tag="OQL90_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1368316.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14739..15734
                     /gene="flk"
                     /locus_tag="OQL90_001496"
     CDS             14739..15734
                     /gene="flk"
                     /locus_tag="OQL90_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1368317.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15731..16909)
                     /locus_tag="OQL90_001497"
     CDS             complement(15731..16909)
                     /locus_tag="OQL90_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1368318.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17229..18449)
                     /gene="fabB"
                     /locus_tag="OQL90_001498"
     CDS             complement(17229..18449)
                     /gene="fabB"
                     /locus_tag="OQL90_001498"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1368319.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18608..20614
                     /gene="mnmC"
                     /locus_tag="OQL90_001499"
     CDS             18608..20614
                     /gene="mnmC"
                     /locus_tag="OQL90_001499"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1368320.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQSFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20735..21013)
                     /locus_tag="OQL90_001500"
     CDS             complement(20735..21013)
                     /locus_tag="OQL90_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1368321.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(21047..21595)
                     /gene="epmC"
                     /locus_tag="OQL90_001501"
     CDS             complement(21047..21595)
                     /gene="epmC"
                     /locus_tag="OQL90_001501"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1368322.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21595..22404)
                     /locus_tag="OQL90_001502"
     CDS             complement(21595..22404)
                     /locus_tag="OQL90_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1368323.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22404..23228)
                     /gene="mepA"
                     /locus_tag="OQL90_001503"
     CDS             complement(22404..23228)
                     /gene="mepA"
                     /locus_tag="OQL90_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1368324.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23232..24317)
                     /gene="aroC"
                     /locus_tag="OQL90_001504"
     CDS             complement(23232..24317)
                     /gene="aroC"
                     /locus_tag="OQL90_001504"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1368325.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24352..25284)
                     /gene="prmB"
                     /locus_tag="OQL90_001505"
     CDS             complement(24352..25284)
                     /gene="prmB"
                     /locus_tag="OQL90_001505"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1368326.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25450..26001
                     /gene="smrB"
                     /locus_tag="OQL90_001506"
     CDS             25450..26001
                     /gene="smrB"
                     /locus_tag="OQL90_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1368327.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(26074..26787)
                     /locus_tag="OQL90_001507"
     CDS             complement(26074..26787)
                     /locus_tag="OQL90_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1368328.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         6612 a         7331 c         6757 g         6108 t
ORIGIN      
        1 ccgcatcaag atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta
       61 attcttccgg cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg
      121 ttttcacaga ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt
      181 ttggttccag tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa
      241 tatcaataaa atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat
      301 gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac
      361 gcgacaataa ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa
      421 ttcagaagta tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat
      481 tccccatgaa aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc
      541 ttaaggaact gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgccc
      601 tcttcttcta ttttcccctg atggaggaaa atgacatgag tagaaacatg gcgagcaaag
      661 cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata
      721 cgcaacactt cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa
      781 acttccggtt ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa
      841 agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc cgacttttgc cagatacttg
      901 accgcccgct cgtgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg
      961 acgttttcca gcaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg
     1021 cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg
     1081 cgcaccagat tgatcgtctg gccattgacc acgatcgacc cttcactcgg tttttcgagg
     1141 aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt
     1201 acatctccgg cattcgcttg cagtgagacc cctttcagca cttcatgttc gccgtagcgt
     1261 ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga
     1321 aggtttcaca tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata
     1381 agagatgatt aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc
     1441 ggcgttaata tcgcgggcta ttttcagcag atccggcaca gtggccgtaa acgccagcgc
     1501 agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc
     1561 agaaggcaaa ataatgcaac gatacatttt aaaagtcgag aagccatagg ctctggcggc
     1621 ttcaatttcc ccatgcagaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc
     1681 gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt
     1741 aaggaattcg gttcccttaa caatctcaag cgtgtatatg ccggaataga acaccagcaa
     1801 ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat
     1861 gtatttatta ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac
     1921 taccgacaaa ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt
     1981 ccacagcagc ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca
     2041 cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacagt ggtgaaaacc
     2101 aggtaaatca cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc
     2161 agttgggtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg
     2221 aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac
     2281 atgatccgcc gaaacacttg cccacgagta aaaccgaacg ccgtcgccgc ctctatatgt
     2341 cctttcggca ctgccataaa agcaccacgg aacgtttcgg taaaataagc accgtagata
     2401 aaaccgagag tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc
     2461 gcctccgtca ccgtgtttag cgcaatctgc aaaccgaaga aaatcagcag catcaacact
     2521 aagtccggca cgccacgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga
     2581 ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag
     2641 ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatat
     2701 aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac
     2761 ccgaagggag cgggctttct ttcaccgatg gcgattagcc accataaaca tcaaaatcga
     2821 agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt
     2881 tcagtgcttc gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa
     2941 acagtttttc atctttaaca gacgggccac cgaatttgta atctttaccg acaggttgtt
     3001 tgaggaaacc ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag
     3061 tcaggtcaga ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg
     3121 cccaatgttc attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc
     3181 ctttcagcga ctcgactgtc ggctgaatgt cagaattttt cgccaccacc aaacgagaat
     3241 cggcagcgta cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa
     3301 gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg
     3361 gattttcgac aaacgtacat tgcgtattga tgcgtttgca taattccttt gccagatcga
     3421 tatcgaagcc aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt
     3481 cggtaccgat gcggatgttt tgcggaatcg cagcaaatgc cgcagttgcg ctggagaagg
     3541 ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt
     3601 ttatagggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc
     3661 aaaatatccc gccccgttag atgtattatt tttcattaaa aatgcttaag tatgcatttc
     3721 aatgcaccac gatagtgcaa caaacctagc gtgcaccatg atgatcattc catcaggtac
     3781 agcttcccag cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc
     3841 ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt
     3901 tcagcatcat ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt
     3961 actgatgagc cagcaaaggc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg
     4021 gcagcaactt catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc
     4081 aaatcctggt tggcataggc caccacatcc acgcctttgc tacgccaggt ctcgttagcg
     4141 taagcttcct gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc
     4201 gttggctgaa tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg
     4261 tcggagaagg caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatggcg
     4321 tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta
     4381 catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa
     4441 tcacctttag catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc
     4501 gtctccggta gcgccgcata gctggaagcc gctacggaga gaccgactaa caaagacaga
     4561 gcgagaatcg acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta
     4621 ttgtgttgtg tgtttgcagg ctctttcatg caggtcttat gccatcttga cgacaacagt
     4681 aacattcaac gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag
     4741 caggtatagc ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat
     4801 ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca
     4861 gcgggcaaag agatcttcag gaaggcttat tgcaaactgg tcaagaacac gattaaccgt
     4921 ctgatttatc acatcatcaa gggattgcgg gcgatgataa aacgccggaa caggaggcat
     4981 aatcaccgca ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg
     5041 tgtttcacgc acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg
     5101 ggtcagtaag ccatcagtat agctatggac aatgccggaa agggttttga ttgaacaggg
     5161 taaaatcacc atccccagcg tctggaaaga accggaagag atgctggcgg aaatatcgcg
     5221 cgcatcgtgc gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag
     5281 ggataaggtc tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc
     5341 gcgcagaacc tgtaataagc gcacgccata aatcgcgccg ctggcaccgc tgatgcctac
     5401 aatgagtcgt ttcataaata gttgccctgt cagacttgca ggcagacttt gcagtatttc
     5461 ggcgggagtt gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt
     5521 tatgcatttc gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac
     5581 gtaacgtatc gaggaaatcg aggtagtcct gatcaacatc tttggtgacg tagacgccgt
     5641 tgaacaccga gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca
     5701 gatcgttcag atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa
     5761 cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg
     5821 ggaagcgaat ttccggggcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg
     5881 ccatctcgat aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga
     5941 cgtttttatc gcggaactcg gcgcggttgg cgttcagttt acggcgcacg gacttacgac
     6001 gcagctgctg gcccggcatg atgaaggtgc ggccaacata gcggttttta acgaagccct
     6061 ggcggtacgg cttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttccg
     6121 ggatcgggat caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcaccca
     6181 gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat tttgtcgata aacgagtccg
     6241 ggcgggcaaa gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt
     6301 gacgggtaaa caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac
     6361 gcaggaaatc aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg
     6421 tacggttctc gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc
     6481 ggaaagcaac cataccgtgg ccgataatca tcgccacaca ggcatatgcg ccgcggatta
     6541 agcggtttgt ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga
     6601 agttgtccag ctcgctagcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga
     6661 tgtggcggcg tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc
     6721 cgttgtgggc aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg
     6781 cgctggagct gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac
     6841 gctgcatatg gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga
     6901 agcagttatt ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca
     6961 ccgttaaggc atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac
     7021 cgcacatacg tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga
     7081 aacttgacga gctttgcaga taatcaaaaa accatctgat gataaaactg aactgcggga
     7141 tcagctgtga tttgctccag tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga
     7201 agagaatggc ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc
     7261 gatcggtgcc tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgatag
     7321 caccaacgat cagggtagcg ataaacagta ccgcgatggc aattccattt cgaaccagtt
     7381 cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga
     7441 aagcacaacc ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa
     7501 ccagagagga aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc
     7561 cacgatttta cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt
     7621 gcgtagggat ttccttccca cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca
     7681 tcgacttttt atgcctttgc ggcatcgtgc aatgcgtgtc ggatgcggcg taaacgcctt
     7741 atccgaccta cggttctaac actgcccaag cctgataaga cgcgccagcg tcgcatcagg
     7801 caagactgta ttaattcggc gtatagccca ttaccacgcc acttaagcca gaaagttgct
     7861 tcaactcacc cagcgaacct ttcagcttat ctttcgaggc atccggccca accagaatac
     7921 gggtaatttt accctgcact ggcgtggatg gcgacgtata aacccgataa ccggcaccgc
     7981 gcagcttacc gacaatctca ttcactttat cggcattttt cagcgcaccc agttgcacaa
     8041 cataggcttt acccgttggc gctgcttttt cttccacgac cggcttcggt tctggcttcg
     8101 gcgcaggtgg tgcttcaacc ttcggtttag gcggctccac cggtttcggc ttcggtgggg
     8161 cgaccggtgc aggttccggt tcaaactcgg tgttattggc tgcaatagtg gccggatcga
     8221 gcgacggtgc tgcggcatca cctgcccgca cctcttccgc tgcgccttcc ggcggctgcg
     8281 tcggcaacgc ctgggtggcg gctggcatca tatcaggctc atcgcgatcg cccgcttttg
     8341 gaaccagcgg gatagccgca aactcatcct gataatgttt tttctgcccg tccagcagcc
     8401 ctggaagtac aatcaccccc agcgccacca gcacgatcgt gcccactaac cgattctgaa
     8461 acttacttgc caccgcttct cctcgcgtca atcacttcca tgacatgtgc gaccgtgtgg
     8521 aaagaaccac acaccagcac ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat
     8581 gcctgcgcaa cgctatcaaa tgatttgccg ttacccaaat gctcaagcag ttgttctgcc
     8641 gtggcaccgc gcggcccttc cagtggcgca caataccagt catcaaccac gcttttcaac
     8701 caggccagag ttccggcaat atctttatca tgtagcatac cgataaccgc cagcacgcgc
     8761 ccgtttttcg gtagcgcttt catacgcccg gtgagatatt ccgccgcatg tggattatgc
     8821 gcgacatcaa aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt
     8881 gcgctggcaa tcccgtcgcg aatggcattt tcactgactt ccagcccgct ggcacgcagt
     8941 gccgccagcg ctgttgcggc attcggttgc gggacaagcg gcaacggcag attttccagc
     9001 gtgccgtgag catcgctaaa cgcccagtca tgatcggtga cggaatagtt ccactcaacg
     9061 ccccgacgtt gtaacagtgc acctttttcc tgcgccacat cagcaatggt agaaggcatt
     9121 tccggctcac cgacaattgc cggttttgcg ctgcgaaaga tgcctgcttt ctcgcgacca
     9181 atactttcgc gatctggccc cagccagtcg gtatgatcca gcgcaatact ggttactacc
     9241 gcgacatcgg catcgacaat attggttgcg tccagacgac cgcccagccc tacttccaga
     9301 atcaccacgt caagttgtgc ctgtttgaac agccacaacg ccgacagcgt accgtactcg
     9361 aaataggtca gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga ggcggtgtgg
     9421 gccgattccg gcaattcctg accctgcaca cgtacgcgct cggtataacg caccagatgc
     9481 ggcgaactgt agacgcccac tttgtaccct gccgccatca gaatcgactc cagcgtacgg
     9541 caggtggtgc ctttgccatt cgtacccgca acggtaaaca caaatggcgc tggtttcaga
     9601 acgccaagac gagccgcgac ctggctcacg cgctcaaggc cgagatcgat agttttactg
     9661 tgcaggtttt ccagataaga aagccacgaa gccagaggcg acgcggcttg aggagtgcgt
     9721 ttgataatca tggtatccgc tgattcgtta cggtgagaat agcaaaaggg cagagccagt
     9781 ggccctgccc ttatcagtta tcaggcctca ggttcctgat ccggtaccgg gggtaccact
     9841 acgccttcac gcggcgcttc aggattcggc gctggcagat tcatcaactt cgccagaatg
     9901 ctcgccagtt tcaggcgcat ttccggacga cggacgatca tgtcgatcga gcctttctcg
     9961 atcaggaatt cactgcgctg gaatcctagc ggcagttttt cgcgaacggt ctgttcgata
    10021 acacgcggac cggcaaagcc aattaacgct ttcggttcag cgatgttgag atcgcccagc
    10081 atggcgaaac ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga gatgtacggc
    10141 aagccgcgct cctgcatttt tgccagtgcc gcagaggttt tcgccatctg catcagcgac
    10201 atcagtgctt cctgcatacg tgcgccacca gaggcggaga agcagatcag cgggcagtta
    10261 tcttccagcg cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga ccccattgaa
    10321 ccgcccataa aggcgaactc gaatgccgca gcgacaaccg gcattccata cagagtgcct
    10381 ttcatcacca ccagcgcatc tttttcgccg gtttctttct gcgcagaagc cagacggtct
    10441 ttatacttct tggagtcacg aaacttcagc acatctttcg gctcaagctc gctacccatc
    10501 tccacaaggc ttccttcatc taacaggcta tgcaggcgat tacgcgctgt catacgcatg
    10561 tgatggtcac acttcggaca gacctcaaga ttacgttcca gctcagcgcg gtataaaacc
    10621 tgaccgcagc tatcacactt agtccacacc ccttcaggaa tgctcgcctt gcgggtggga
    10681 gtaatgttgc ttttaattcg ttcaatccag ctcattaggg acctttctgt ctgaacctgg
    10741 ttcgatgcca gttttatctt tggggacgca taatgccatt tttgccccca acagaccatg
    10801 aatgttgcac attaaaacat aacagcccga aactttggat aaaaaagtgg tcgaaccgcg
    10861 gagttacttt ttattttgcg gcgcgtgccg cagcgcgttt gtgacgaata aattcgaaaa
    10921 ccccaggcaa tattgaaaca aaaataatgc caacaatcat cagcttaagg ttgctctgaa
    10981 tgaaggggag catgccgaag aaataacccg cgtaggtaaa aatcagtacc cacaacagtg
    11041 cgccgatcac gttataagcg gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa
    11101 acggcgcgaa cgttctgacg atcggtacaa aacgggcgag aataatcgtt ttgccgccat
    11161 gtctctcgta aaactgatga gtcttatcaa gataactgca acgaaagatt ttcgaatccg
    11221 ggttactgaa cagtttgtca ccaaataacc gtccaatggt atagttcact gcatcaccga
    11281 caatagcggc aatcaacata agcaccacca tcatatggac attgagatcg ttagtttcca
    11341 gcgacgccaa tgcaccagca acaaacagca gcgaatcacc aggcaggaat ggcgttacca
    11401 ccagccccgt ttcgcagaac agaatcaaaa acagaatggc ataaacccag acgccgtact
    11461 ccgcgaccag ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa
    11521 tcaggtccat atttgcctat gccttgtact cgtcatcttt caggctgtaa ctacgttggc
    11581 tacagcctga aatgttccga gcattattct cgttagtccg ccagaaatag cgggcccatt
    11641 ggcggttttg gaagatcata ccggtcaggg taatccaccg cgaccagata cagcccttcc
    11701 gcttttgccg ttgctgccgc cagcgttctg tcctttgccg ccagcaactc tgctatccag
    11761 ctctccggct ggttgtgggc accgacttcc atcaggctgc cgacaatatt cctgaccata
    11821 tgatgtacaa aggcattcgc tttgatatct accaccacat aaggaccgtg acgcgtgaca
    11881 ttaatatgca taacgttgcg ccacggggtt cgggactggc actgcaccgc acggaacgag
    11941 gtgaaatcat tctcgcccag caagcattgc gcagcccgat gcatccgttc agcgtccagc
    12001 ggttcgtaaa aatgggttac ccctttactc agtaccgccg ggcgcagccg atgattgtag
    12061 atgatgtagc gataacggcg agccgtggcg ctaaatcggg catgaaaatc atcaggtaca
    12121 gctttaaccc aacgcacggc gatgtcatca ggtaaattcg catttacgcc caaggtccac
    12181 gccgcgtctt tgcgctgcgc ggttgtttcg aaatgcacca cctgcccggt accgtgtacc
    12241 cctgcgtcag tacgcccggc gcagaagacg gtgatgggtt cgttcgccac ctgggagagc
    12301 gccttttcca gcttctcctg cacactgcgg acttcattct gccgttgcca gccgtaatac
    12361 ttactgccgt cgtactcaat gcccagcgca attttataaa ctggcagttg ttgctggtcg
    12421 gacattagta cagatactcc tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc
    12481 cgccaaagcg aacgttatcg gccaccgacc agaactggac ttgctccggc ataccgtagt
    12541 cattacgcac gcagccaaca gaaagatgcg gagtacccga agcatcccct acctgagtcg
    12601 ggaattcgtt ctcttcagag agcacaatat cttcgccctg agcaaacgca tcacgcgctt
    12661 cttctgccgc cagcggacgc agtgcttcaa agttgaccat ctgggcatga ccgtagaata
    12721 ccggtgcctg gacgacgctt gccgaaatca tcagcccttc gtcctgcagg attttgcgta
    12781 cttcgtcaac gatacgacgt tcttcacgca cgctaccttc gctatccggc aacaacggca
    12841 gcatattgaa cgccagttga cgaccgaaga aatcttcttc gtcaatcgga atgccgttga
    12901 gcaatttcgc actctgcccc gctaacgcat cgaccgcttt tttgccctgg gcggaggctg
    12961 aaatcaggct ggtaacactg atacgcgaca aaccgccctg atcgattaac ggtttcagtg
    13021 ccgccagcag ctggctggtc agactgtctg gtacggcgat gacattccgg ttgcggtaat
    13081 cggtcagtac aaacgggttt acttccggca ccaccagcgg tacgtcgggt tcgagagcaa
    13141 acagtccgct gctgtcgatc accaggcaac ctgagttggt cgcttcttca acccaggcag
    13201 cggtagcttc tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag tcgaattcag
    13261 cggcatcctg cacggtgatt gttttaccac caaagcgcag ttgttcgcct gcgctttcgt
    13321 tacgtgccag tgcataaatt tccccaaccg ggaactgacg ttcagccagc gtttcaagca
    13381 gggcttcgcc cacagcgcca gttgcgccca ggacggcaat gttccagcct tcagacatgg
    13441 tggtttactc cagaaatagc aaagctccct gccaatgtta cagcagagag catgaagaag
    13501 agattaacgt gccggatgat gaacggcgtt aaaacccagt ttacacagca atgatgccgc
    13561 actggcgtca tcacaaatta catacagaga tgaccattca cggcgctcaa gatagttttt
    13621 gcgcagttta tcgaactcac ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg
    13681 gcgcacatca tacaccaaat gcaccagcct tttcagcgtc ggttgatcga gcgggccatg
    13741 cagcgtaatg cgaccaaact ctggcgcggg cagtaatgta tccagcgcaa cgtgctgttc
    13801 atgcccaata aacttgctat aagcttcaaa cacttgcgta gtaccgcgtg ctttaccttc
    13861 cagggtataa cctgcgatat gcggcgtgcc gatatccact tttgtcagca actccacgtt
    13921 aagttccggt tcgccttccc agacatccag cactacgctt aacttctggc cttcattcag
    13981 gcaggtcagc aacgcagtat tatcgacgac tgcgccacgg caggcgttaa tcagaatcgc
    14041 tcccggcttc aggctacgga tcagtttttc atccgccaga tgcagcgttt tgtacggccc
    14101 atctttgaag agtggcgtat ggaaagtcag aatatccgca tgctgaacta attcatccag
    14161 cgagcggaaa tcaccctcat ccccacggtc agcgcgaggc ggatcgcaaa gcaatgtttt
    14221 aatccctaac gcttccagtc gcgcctgcaa tcgacgtcca acgttaccaa cgcccacgat
    14281 ccccaccgta cggtcgtgca gtgaaaatcc atcgcgttcg gcaagcatca gtagtgagga
    14341 gaaaacatat tccaccaccg caatcgcatt acagccaggt gcagcggaaa aaccaattcc
    14401 cgcctgcttc aaccatgctt catcgacatg gtcggtcccc gcagtggcag tgccaacaaa
    14461 tttaatgggt tttcctgcca gcaaagattc attcactttc gtgaccgaac gcaccatcag
    14521 cgcatccgcg tctgccagtt gagcgacggc gattggacgc ccgggaaccg cggtcacctc
    14581 acccaaacgg ctaaataagt cgcgggcata aggcatattt tcatcaacaa ggattttcac
    14641 gtttgtgtta cctgtatgag acgagagtta accggacaag tgtgccataa tctcgcggcc
    14701 aggcatactt gcgaagattt caggtataag gatacgtaat gatacaacct atttccggcc
    14761 ctcctcctgg gcaaccacca ggtcagggag ataacctgcc gtctggcgcg ggcaatcagc
    14821 ctttatccag tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg acctcactga
    14881 cgcaacagca aagagcagaa ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag
    14941 attcaccgct gctttcgcgt cacttccctg ccgctgagca taatctggcg caacgtctgc
    15001 tggccgcgca aaaaagccat tctgcccgcc agcttttagc gcaattaggg gagtatttac
    15061 gtctggggaa taatcgtcag gcggtcacgg attatatccg tcataacttt ggtcagacgc
    15121 cgctgaatca gctctcaccg gagcaattaa aaaccattct caccctgttg caggaaggga
    15181 agatggttat tccgcaaccg cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg
    15241 agcacaatgc gctaaaacag ctggtgacca aacttgcggc ggcaacgggg gaacccagca
    15301 aacagatctg gcaatcgatg ctggaacttt ccggggtgaa agatggcgag ttaattccag
    15361 cgaaactgtt taaccatctg gtgacctggc tacaggcgcg tcagacgcta agccagcaaa
    15421 atacgccgac gctggaatca ctacagatgg cgctaaaaca acctttagat gccagtgaac
    15481 tggcggcgtt atcggcatat atccagcaaa aatatggcct ttctgcgcaa tcatcgcttt
    15541 cttctgccca ggccgaggat attcttaatc agctttatca acggcgggtt aaagggattg
    15601 atccgcgtga tatgcaaccg ctgcttaatc cttttccacc gatgatggac acgttgcaaa
    15661 atatggcaac gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg atgctggtct
    15721 ggctggttcg ttaaccccga cgaaatgaca gtatcgtgac aataataccc agcaccgcag
    15781 agatcgcccc ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc attcccgcca
    15841 gcggcccgga gacgccaagg gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc
    15901 cgcgaacttg tgaggggacg cgtttaacca cctccacgcc cagcgcagga aagataagcg
    15961 aacatccggc tccggttaac gccgcgcccg ctaatgcgac ccatgcacct ggggcttgcc
    16021 agagcagcaa caagcccacc gtttctacaa gcagagagac aatcgccact ttcacgccgc
    16081 caaaacggtc cggcatccag ccaaacatga cgcgcatcac gacaaatgcg ccgccaaacg
    16141 cggtaagagt aaagcccgcc atcgcccatc ctttgctggc aaagtagagc gaaacgaaag
    16201 tcccgataac cgcaaaacca acgccttgta gtgccagccc taaccctggt ttccagataa
    16261 gcccgacaac gctccacagc gatggacgtt ctcccgccag ggccggtact ttgcgcactg
    16321 tgccgttaca ggcccacgcc agtaagggta ataccattgt ggtgatcgcc agtgcggcaa
    16381 aaccgtaatg gctatgaatc aacaggccaa gcggcgcacc aacagcgagg gcaccgtaaa
    16441 tcgccattcc gttccatgac atcactttgc cagagtgttt tgcccctacg atgcctaatc
    16501 cccaggtcag agcgcctgtc agtaactggc tttcaccaaa gccaagaatc aaacgcccga
    16561 cgaccaacag ggcaaatttc aacggtgcgg agacaggcaa aatcgccgcc agcagcaacg
    16621 cgccgccagc cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt gcaccatatt
    16681 gatcggccag tcgcccggca tagccacgcg tcagcaccgt agccagaaac tgaatcccga
    16741 cggcaatccc gaccatggta ttgccatagc ccagatcatg atgaacaaac agcgggataa
    16801 ccggcaacgg cagccctacg gtcatgtagg tgagaaaaac cgcaaaagcg atgcggaaga
    16861 gcgaaaaatt ggcagaagat cgtgtttcgg tttggcttac agcagtcatg cattactcca
    16921 gaatgcagcg caaggcgagg agtatccccg tctcatctct ctggtttcag ggttacagtg
    16981 cgttggcagg atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg
    17041 cctggaactg gcggcgattg tcaatgatgt gaaaaaggga accatcaggt tcccttttgc
    17101 gttagtgccg gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc
    17161 gacctactcc gactactccg actactccga ctactccgac tactccgact actccgacct
    17221 actgcgaatt aatctttcag cttgcgcatt accagcgtgg cgttggtgcc gccgaagccg
    17281 aagctgttag acataacggt ggtcagttcg cgatcggtcg tttcggtcac gatgttcaga
    17341 cctgccgcct gctcgtccag ctcttcaatg ttgatgctcg gggcgataaa gccgtgttcc
    17401 agcatcagca gagagtagat agcttcctgt acgccagcag cgcccagaga gtgaccggtc
    17461 atggctttgg ttgcagaaat cgccgggctc ttatcgccga acacttcacg gatagctgcc
    17521 agttctttca cgtcgccaac cggagtcgaa gtaccatgag agttcaggta atcgattggg
    17581 gtatcaacgc cgtgcatcgc catcttcatg cagcgtactg cgccttcgcc agacggagca
    17641 accatgtctg caccatcaga ggttgcgccg tagccaacga tttcagcata gatgtgagca
    17701 ccacgcgcca gcgcgtgttc cagctcttca accactacca taccgccgcc gccagcgata
    17761 acgaaaccgt cacggtgagc gtcgtaagta cgggaggctt tttccggggt gtcgttgtat
    17821 ttagtagaca gcgcacccat cgcgtcgaat tcgcaggcca tttcccagca cagctcttcg
    17881 ccgccgccag caaacacgat gtcctgtttg cccagttgga tctgctctac tgcgttaccg
    17941 atacagtgtg cggaagtcgc acacgcggag ctgatggagt agttaacgcc atgaatttta
    18001 aacggcgtgg cgaggcaggc agaaacgccg gatgccatcg ctttggtgac cacatacggg
    18061 ccaaccgctt tcaggccgcg cgggccgcgc attgcgtcag cgccgaatac ctggaaacgc
    18121 ggggaaccgc cgccagaacc tgcaatcagg ccaacgcgcg ggttattctg gtaagcttcc
    18181 ggagaaaggc ccgcatctgc gattgcctgc tccatagaaa ggaatgcata aatggatgcg
    18241 tcgctcataa agcgcacaac tttgcggtca atgaggccag tggtatccag ttttacgttg
    18301 ccccagacgt ggctacgcat gccggaatcc ttaagctcct gagagaaagt gatccctgaa
    18361 cgtccttcac gcagagatgc caggacttcc tgctggttat taccgatgct ggaaacaatg
    18421 cccaggccag taatcactgc acgtttcatt caatacctct gtaagtcgca catagagtaa
    18481 gtttcgaatg cacaatagcg tacacttgta cgccgaacaa gtccgatcag ccatttaata
    18541 gagaaatttg cgcagcctta cacacatcgc taagatcgag ccaccgcctg taagacgagt
    18601 aacttacgtg aaacactact ccatacaacc tgccaacctc gaatttaatg ctgagggtac
    18661 acctgtttcc cgagattttg acgatgtcta tttttccaac gataacgggc tggaagagac
    18721 gcgttatgtt tttctgggag gcaaccaatt agaggtacgc tttcctgagc atccacatcc
    18781 tctgtttgtg gtagcagaga gcggcttcgg caccggatta aacttcctga cgctatggca
    18841 gtcatttgat cagtttcgcg aagcgcatcc gcaagcgcaa ttacaacgct tacatttcat
    18901 tagttttgag aaatttcccc tcacccgtgc ggatttagcc ttagcgcatc aacactggcc
    18961 ggaactggct ccgtgggcag aacaacttca ggcgcagtgg ccaatgccct tgcccggttg
    19021 ccatcgttta ttgctcgatg aaggccgcgt gacgctggat ttatggtttg gcgatattaa
    19081 cgaactgacc agccaacagg acgattcgct aaatcaaaaa gtagatgcct ggtttctgga
    19141 cggctttgcg ccagcgaaaa acccggatat gtggacgcaa aatctgttta acgccatggc
    19201 aaggttggcg cgtccgggcg gcacgctggc gacatttacg tctgccggtt ttgtccgccg
    19261 cggtttgcag gacgccggat tcacgatgca aaaacgtaag ggctttgggc gcaaacggga
    19321 aatgctttgc ggggtgatgg aacagacatt accgctcccc tgctccgcgc cgtggtttaa
    19381 ccgcacgggc agcagcaaac gggaagcggc gattatcggc ggtggtattg cctgcgcgtt
    19441 gttgtcgctg gcgctattac ggcgcggctg gcaggtaacg ctttattgcg cggatgaggc
    19501 ccccgcactg ggtgcttccg gcaatcgcca gggggcgctg tatccgttat taagcaaaca
    19561 cgatgaggcg ctaaaccgct ttttctctaa tgcgtttact tttgctcgtc ggttttacga
    19621 ccaattaccc gttaaatttg atcatgactg gtgcggcgtc acgcagttag gctgggatga
    19681 gaaaagccag cataaaatcg cacagatgtt gtcaatggat ttacccgcag aactggctgt
    19741 agccgttgag gcaaatgcgg ttgaacaaat tacgggcgtt gcgacaaatt gcagcggcat
    19801 tacttatccg caaggtggtt ggctgtgccc agcagaactg acccgtaatg tgctggaact
    19861 ggcgcaacag cagggtttgc agatttatta tcaatatcag ttacagaatt tatcccgtaa
    19921 ggatgactgt tggttgttga attttgcagg agatcagcaa gcaacacaca gcgtagtggt
    19981 actggcgaac gggcatcaaa tcagccgatt cagccaaacg tcgactctcc cggtgtattc
    20041 ggttgccggg caggtcagcc atattccgac aacgccggaa ttggcagagc tgaagcaggt
    20101 gctgtgctat gacggttatc tcacgccaca aaatccggcg aatcaacatc attgtattgg
    20161 tgccagttat catcgcggca gcgaagatac ggcgtacagt gaggacgatc agcagcagaa
    20221 tcgccagcgg ttgattgatt gtttcccgca ggcacagtgg gcaaaagagg ttgatgtcag
    20281 tgataaagag gcgcgctgcg gtgtgcgttg tgccacccgc gatcatctgc caatggtagg
    20341 caatgttccc gattatgagg caacactcgt ggaatatgcg tcgttggcgg agcagaaaga
    20401 tgaggcggta agcgcgccgg tttttgacga tctctttatg tttgcggctt taggttctcg
    20461 cggtttgtgt tctgccccgc tgtgtgccga gattctggcg gcgcagatga gcgacgaacc
    20521 gattccgatg gatgccagta cgctggcggc gttaaacccg aatcggttat gggtgcggaa
    20581 attgttgaag ggtaaagcgg ttaaggcggg gtaatctgct ctcgcagtgt ttgccggatg
    20641 cggcgtgaac gccttatccg acctacgtgt gacgtgtagg cctgataaga cgcgataagc
    20701 gtcgcatcag gcatggtgct ccagacgccg caacttactg ctgtgacgcc tgctgaaaca
    20761 ggttttccca catatcggtc accagcgcct ggtcacgcgg cgacaattca ccggcaccaa
    20821 tggctttttc cagactctgg ctaacggtcg tatgcaccgc ctgagcggag tggtcatcac
    20881 cactttccag ttctgcgatg gctaatgtca ggtggccacg caaataccca ctggcaaaca
    20941 actcatcatc actggcatgg tcaatcatgc cgtcgattaa tgccagaatg cgtgattcaa
    21001 actccgcgat catcttcttt cctcattgta aaacgtggca ccggcttcag ttgagcgctt
    21061 ccggccacgg gaactgttcc gccgtaagtt ccggcgtgtg ataataattt tgtagtgctt
    21121 taatgaagcg tgccggacgc tcgggaatgc catttgtcag ataatccatc acctgcgcat
    21181 gtacccgccg ttgaaacacc acacgatccg gttcgaagtc gccttccaga ttgtcgcaac
    21241 tgacgttaaa cggatatccc gccgccacac agaacaacca gtcgagtgcc tgcggcttca
    21301 cttcaacatc ttcaaactgg ctttgcgttt gggcatcacg cccatccggg caataccagt
    21361 agccgaaatc aaccagctca cggcgcgctt ttccggcaat acaccagtgc gaaatctcat
    21421 gaattgcgct ggcataaaag ccgtgggcaa agacaattcg gttatacggt acttccgcat
    21481 cagcaggaag atagatcggt tcgtcgtcgc ctttaatcag acgggtatta aattcatcgg
    21541 caaagcagct attaaaaatt tcaatcaact gctcatagtg gtgtgtactg ttcattagtt
    21601 catccccaac cagtggagga tctcctgtcc gtggctatcg taaagtaatt tggcactcat
    21661 caccgccgag acgataacga tcatcgggcg gatcagtttt tgtcctttgc tcagcaccag
    21721 tcttgaaccc atgcgcgcgc cgaggaactg ccccaccagc atgacaaagc ccgtcgccca
    21781 aatcactttg ccgccgagaa taaacagcag cagaccgcca atgtttgacg ttgcgttgag
    21841 taatttggcg tgagccgtgg ctttggcgag gttgaatccg cacagcgtaa cgaaggccag
    21901 cgcgtaaaac gatccggcag ccgggccaaa gaatccgtca taaaaaccga cgcagccacc
    21961 ggcaattaac gcgaagggta aaccgtacat ccggcgctgg cgatcttctt cacccagctt
    22021 tggcatcagc aagaaataga ggccgataca aattaccaga atgggcaaaa tctgccgtaa
    22081 gacatcagcc tgaacgtatt gcaccagcag tgcgccgctc attgagccgg caaaggtcat
    22141 ggcgatattg agtttctgat cgcttaaact aaccactttg cggcgaataa agtagatagt
    22201 ggcggaaata gagccgccgc aggcttgcag tttattggtt gccagcgcgt tagcgggaga
    22261 cattcccgca gccatcaatg ccggaatggt gagtaaccca ccgccaccgg caatcgagtc
    22321 gataaatccc gccagcatgg cgacaaaaaa gagaactccc agcaacagcg gggaaaccat
    22381 aaacaggcta ttaaacgttt ccattagatc acgtgctcat ccagtagcgc ctggcaggaa
    22441 ggcggcaacg gaggcggtgt cttcttctca ggctttgttg ttcctggttt tggaggtgca
    22501 aaccagcttt gcagttctgc cccgcaacca tcgcctggtg gcggtaaagg ttgatcttca
    22561 cactccagac tatcggcagg gcaacgtaat cgtacatgca tatgcgcgcg atgctggaac
    22621 cagggtcgca ctttgcgcaa ccagtcgcga tcggtgcccg catcaaggca aagttgttgt
    22681 ttaatcgccg gattaacaaa aatgcgcgtg acgtctttgt cctgggcggc gagtttgatc
    22741 aagctgaaaa tttctggctt ccacagcgtg gagacaacgt gtttaccgtc gcgggaaact
    22801 aagtccagtg cttgcgggcg caagagctgc gcggaggtcc agcgagtttt cggcagttgc
    22861 agaaagatat cgacatccag tccggtctgg tggctggcat gaccgccgtt gaaacgccca
    22921 ccagcgggca tccccatatc gccaatcagc accgtaccca tgcccagatt gctcacctgg
    22981 ctactcagac gctggataaa catcaccaga tccgggtgac cgaaatagcg acgctgatcg
    23041 gtacgcatga cctgataatg ttcggactgt atcggcaacg tgtcagcgcc gacaatacag
    23101 ccattagaaa aactgcctat cgattgtgcg ctacccggca caggttgggt tattttttgc
    23161 cacggcgtcg ctgccaagct agcgctactg gcaagcagag ccagcagcgc aatcgcggtt
    23221 ttgttcattt tttaccagcg tggaatatca gtcttcacat cggcattttg cgcccgctgc
    23281 cgtaacaggt gatccattaa aacgatcgcc agcatcgctt cagcgatcgg cactgcacgg
    23341 atcccgacac agggatcgtg acgacctttg gtgatcatct caacttcttc gccaaagcgg
    23401 ttaatagtgc gccccggcac ggtaatgctg gaggtcggtt tcagcgccat atgagcaatg
    23461 atttgctgcc cgctgctgat accgccgaga atgccgcccg catggttgct ctggaaaccg
    23521 tctttggtga tttcgtcgcg gttctggctg ccgcgcagcg ccactacgtc aaagccgtcg
    23581 ccaatttcca cgcctttcac cgcgttgatg ctcatcagcg catgggcgat gtcggcatcc
    23641 aggcggtcaa ataccggctc gccaagtccg gcggggacgc cactggcaac aacggtgact
    23701 ttcgcgccga tggagtcgcc ctcttttttc agcgcgcgca tcagttcatc taacgcgtcg
    23761 attttgtccg gatccgggca gaagaacgga ttttgttcaa ccagcgacca gtctttgatt
    23821 tccagcggaa tgtcgcccat ctgggtcaag cagccgcgga tttcaatacc aaatttctcg
    23881 gcgagatatt ttttggcaat cgcccctgcc gccacgcgca tggcggtttc gcgggcggaa
    23941 gaacgtccac cgccgcgata atcgcgcaga ccgtattttt gttcgtaggt gtaatcagca
    24001 tggcctgggc ggaaaacgtc tttaattgcg ctgtaatcct gagagcgctg gtcagtgttt
    24061 tcgatcaaca agccaatgct ggtgccagta gtaacgcctt caaaaacacc agagagaatt
    24121 ttgacctgat ccggctcgcg gcgctgggtg gtatagcgcg atgtcccagg gcgacgacgg
    24181 tcgaggtcat gttgcaggtc cgcttccgtc agcagaatgc ctggcggaac accatcgacg
    24241 atgcagccga gcgccagccc atgcgattcg ccgaaggtgg ttacgcgaaa gagttgtcca
    24301 attgtgtttc cagccatcac ggctccgtta ttgttgtgtt tgcgtgttta cttaatcttt
    24361 ataaatcgcg aaatattcac gggccgccag cagctgctct ttggtgagca taaacacacc
    24421 atcgccgccg ttatcaaact ccagccaggt gaacggaaca tccggatatt gttccataag
    24481 atgtaccatg ctgttgccga cttcacaaat caacacgcca tcatcagcaa ggtaatctgc
    24541 cgcgtttcca agaatgcgac gcgtcagttt aaggccgtcg gtgccagatg ccaggcccag
    24601 ttccggctcg tgacggtatt cgtttggcag gtcggacata tcttccgcgt cgacatacgg
    24661 cgggttagtg acaatcagat cgtactgcac tttcggcaag tcgcggaaca gatcggaacg
    24721 aatcggaatg acgttgtgga tcagaccgtg ttcttcgatg ttctgttcag caaccgccag
    24781 cgcgtcggga gagatatcca ccgcgtcgac ttctgcatcc gggaaggcat aagcacaggc
    24841 aatggcgatg cagccgctac cggtacacat atctaaaata tgctgcggtt gtttgctgat
    24901 gagtccggca aatttattgt tgatcagttc accaatcggc gagcgcggca ccagcacgcg
    24961 ttcatcgacg taaaattcat gaccgcagaa ccaggctttg ttggtcaggt aagccaccgg
    25021 aatgcgctcg ttgacgcggc ggatcacgcg ttcaacaata cgatgttttt cgctggaggt
    25081 cagacgcgcg gtgcgcatat cttccgggat atccagcggc aggtagagcg atggtaacac
    25141 cagctgtacg gcttcatccc acgggttatc ggtgccgtga ccgtaccaga tatttgccgc
    25201 gctgaagcgg ctcactgacc agcgcaacat gtcctgaatg gtttgcagct catttactgc
    25261 ttcatcaacg aaaattttat ccacgtattc ctccagggca tgatcgcaat aatttcggcg
    25321 gctagtttgc catgaagatg acgataaatc agcattcacg cgcggtgagt gaggaaaaat
    25381 acgtttaaaa cgatcgattg tgctacgagt cgggtaaact gtaggaaaat tagaaataga
    25441 gacagataaa tgaaaaagaa aacaacactt agcgaggagg accaggctct gtttcgccag
    25501 ttgatggcgg ggactcgcaa gattaagcag gacacgattg tccaccgacc gcagcgtaaa
    25561 aaaatcagcg aagtgccggt gaaacgcttg atccaggagc aggctgatgc cagccattat
    25621 ttctccgatg agtttcagcc gttattaaat accgaaggtc cggtgaaata tgttcgcccg
    25681 gatgtcagcc atttcgaggc gaagaaactg cgccgcggcg attattcacc ggaattgttt
    25741 ttggatttac acggtctgac gcagctgcag gccaagcagg aactgggcgc gttgattgcc
    25801 gcctgtcgcc gcgaacatgt gttttgtgcc tgcgtgatgc atgggcacgg gaagcatatt
    25861 ttgaagcaac aaacaccgct gtggctggca caacatccgc atgtgatggc atttcaccag
    25921 gcaccgaaag agtacggcgg tgatgcggct ttgttggtgt tgattgaagt ggaagagtgg
    25981 ttgccgccgg agttgccctg aggagttgag cgtgttgtgc caggagcgcg gaatggcagg
    26041 ggcgaatgac cctgccaggt gagaaaaggt gcgctaatac atattcagta tgtaagtgaa
    26101 tcggcaggta gaatctatgg gctgtcgaga tgtcgtgtcc acgcgtagct tcatatcatg
    26161 cagttgcctg gacgtaatcg gttgcggctc aggcgtcacc gtcactttgg cattatcaca
    26221 gccggaagtc gagaaggtca tctgctgtgc gataaaacgg cagtagacac cacgctcatc
    26281 aaaattattt ccgttacaca gtttgccatt ttccatcagg aaagtgggca gtgtttgctc
    26341 aataacacca cctgttgact gcaacctgac gactttgtta tgtacaacat aattttcatt
    26401 ccaggaaaca tcatacggtg ccaggggcac cgccggacat gaagaactca ccttcgttgg
    26461 gccaatatag tcaggaaaat catcatcata cgttttagcg aagggatctc tactcttaac
    26521 aaacgacgac acaatccacg gacaaccatg taaattccca tcgtaataaa caaacatctt
    26581 taattcatta catgtccatc ccgttttggt ctggctatca tactgctcta atccaggaca
    26641 ggctgacatg gataccctgc tgtccgaaat tttcgacata gatggttgag tagaactatc
    26701 gccagtccac gaaactaaat ccggctgtga attgggcatg aatgtagcct taaatatagc
    26761 gtaattaact gaatctggcg tcgctataat cagatgaaac tcaaactc
//
LOCUS       DAJVOY010000041        25131 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVOY010000041 DAJVOY010000000
VERSION     DAJVOY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.041"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..393)
                     /locus_tag="OQL90_001508"
     CDS             complement(<1..393)
                     /locus_tag="OQL90_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1368329.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKN"
     gene            772..1083
                     /gene="rpsJ"
                     /locus_tag="OQL90_001509"
     CDS             772..1083
                     /gene="rpsJ"
                     /locus_tag="OQL90_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1368330.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1116..1745
                     /gene="rplC"
                     /locus_tag="OQL90_001510"
     CDS             1116..1745
                     /gene="rplC"
                     /locus_tag="OQL90_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1368331.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1756..2361
                     /gene="rplD"
                     /locus_tag="OQL90_001511"
     CDS             1756..2361
                     /gene="rplD"
                     /locus_tag="OQL90_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1368332.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2358..2660
                     /gene="rplW"
                     /locus_tag="OQL90_001512"
     CDS             2358..2660
                     /gene="rplW"
                     /locus_tag="OQL90_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1368333.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2678..3499
                     /gene="rplB"
                     /locus_tag="OQL90_001513"
     CDS             2678..3499
                     /gene="rplB"
                     /locus_tag="OQL90_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1368334.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3516..3794
                     /gene="rpsS"
                     /locus_tag="OQL90_001514"
     CDS             3516..3794
                     /gene="rpsS"
                     /locus_tag="OQL90_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1368335.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3809..4141
                     /gene="rplV"
                     /locus_tag="OQL90_001515"
     CDS             3809..4141
                     /gene="rplV"
                     /locus_tag="OQL90_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1368336.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4159..4860
                     /gene="rpsC"
                     /locus_tag="OQL90_001516"
     CDS             4159..4860
                     /gene="rpsC"
                     /locus_tag="OQL90_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1368337.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4873..5283
                     /gene="rplP"
                     /locus_tag="OQL90_001517"
     CDS             4873..5283
                     /gene="rplP"
                     /locus_tag="OQL90_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1368338.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5283..5474
                     /gene="rpmC"
                     /locus_tag="OQL90_001518"
     CDS             5283..5474
                     /gene="rpmC"
                     /locus_tag="OQL90_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1368339.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5474..5728
                     /gene="rpsQ"
                     /locus_tag="OQL90_001519"
     CDS             5474..5728
                     /gene="rpsQ"
                     /locus_tag="OQL90_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1368340.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5893..6264
                     /gene="rplN"
                     /locus_tag="OQL90_001520"
     CDS             5893..6264
                     /gene="rplN"
                     /locus_tag="OQL90_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1368341.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6275..6589
                     /gene="rplX"
                     /locus_tag="OQL90_001521"
     CDS             6275..6589
                     /gene="rplX"
                     /locus_tag="OQL90_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1368342.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6604..7143
                     /gene="rplE"
                     /locus_tag="OQL90_001522"
     CDS             6604..7143
                     /gene="rplE"
                     /locus_tag="OQL90_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1368343.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7158..7463
                     /gene="rpsN"
                     /locus_tag="OQL90_001523"
     CDS             7158..7463
                     /gene="rpsN"
                     /locus_tag="OQL90_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1368344.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7497..7889
                     /gene="rpsH"
                     /locus_tag="OQL90_001524"
     CDS             7497..7889
                     /gene="rpsH"
                     /locus_tag="OQL90_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1368345.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7902..8435
                     /gene="rplF"
                     /locus_tag="OQL90_001525"
     CDS             7902..8435
                     /gene="rplF"
                     /locus_tag="OQL90_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1368346.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8445..8798
                     /gene="rplR"
                     /locus_tag="OQL90_001526"
     CDS             8445..8798
                     /gene="rplR"
                     /locus_tag="OQL90_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1368347.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8813..9316
                     /gene="rpsE"
                     /locus_tag="OQL90_001527"
     CDS             8813..9316
                     /gene="rpsE"
                     /locus_tag="OQL90_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1368348.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9320..9499
                     /gene="rpmD"
                     /locus_tag="OQL90_001528"
     CDS             9320..9499
                     /gene="rpmD"
                     /locus_tag="OQL90_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1368349.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9503..9937
                     /gene="rplO"
                     /locus_tag="OQL90_001529"
     CDS             9503..9937
                     /gene="rplO"
                     /locus_tag="OQL90_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1368350.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9945..11276
                     /gene="secY"
                     /locus_tag="OQL90_001530"
     CDS             9945..11276
                     /gene="secY"
                     /locus_tag="OQL90_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1368351.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11308..11424
                     /gene="rpmJ"
                     /locus_tag="OQL90_001531"
     CDS             11308..11424
                     /gene="rpmJ"
                     /locus_tag="OQL90_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1368352.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11571..11927
                     /gene="rpsM"
                     /locus_tag="OQL90_001532"
     CDS             11571..11927
                     /gene="rpsM"
                     /locus_tag="OQL90_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1368353.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11944..12333
                     /gene="rpsK"
                     /locus_tag="OQL90_001533"
     CDS             11944..12333
                     /gene="rpsK"
                     /locus_tag="OQL90_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1368354.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12367..12987
                     /gene="rpsD"
                     /locus_tag="OQL90_001534"
     CDS             12367..12987
                     /gene="rpsD"
                     /locus_tag="OQL90_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1368355.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13013..14002
                     /gene="rpoA"
                     /locus_tag="OQL90_001535"
     CDS             13013..14002
                     /gene="rpoA"
                     /locus_tag="OQL90_001535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1368356.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14043..14426
                     /gene="rplQ"
                     /locus_tag="OQL90_001536"
     CDS             14043..14426
                     /gene="rplQ"
                     /locus_tag="OQL90_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1368357.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14533..14901
                     /locus_tag="OQL90_001537"
     CDS             14533..14901
                     /locus_tag="OQL90_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCS1368358.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14912..15337
                     /gene="zntR"
                     /locus_tag="OQL90_001538"
     CDS             14912..15337
                     /gene="zntR"
                     /locus_tag="OQL90_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCS1368359.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15393..15611
                     /gene="arfA"
                     /locus_tag="OQL90_001539"
     CDS             15393..15611
                     /gene="arfA"
                     /locus_tag="OQL90_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCS1368360.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15608..16018)
                     /gene="mscL"
                     /locus_tag="OQL90_001540"
     CDS             complement(15608..16018)
                     /gene="mscL"
                     /locus_tag="OQL90_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCS1368361.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16148..17524)
                     /gene="trkA"
                     /locus_tag="OQL90_001541"
     CDS             complement(16148..17524)
                     /gene="trkA"
                     /locus_tag="OQL90_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1368362.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17546..18835)
                     /gene="rsmB"
                     /locus_tag="OQL90_001542"
     CDS             complement(17546..18835)
                     /gene="rsmB"
                     /locus_tag="OQL90_001542"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1368363.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRHH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18881..19828)
                     /gene="fmt"
                     /locus_tag="OQL90_001543"
     CDS             complement(18881..19828)
                     /gene="fmt"
                     /locus_tag="OQL90_001543"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1368364.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19843..20352)
                     /gene="def"
                     /locus_tag="OQL90_001544"
     CDS             complement(19843..20352)
                     /gene="def"
                     /locus_tag="OQL90_001544"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1368365.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20482..21606
                     /gene="dprA"
                     /locus_tag="OQL90_001545"
     CDS             20482..21606
                     /gene="dprA"
                     /locus_tag="OQL90_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1368366.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21578..22051
                     /locus_tag="OQL90_001546"
     CDS             21578..22051
                     /locus_tag="OQL90_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1368367.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22080..22622
                     /locus_tag="OQL90_001547"
     CDS             22080..22622
                     /locus_tag="OQL90_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCS1368368.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22627..23199
                     /gene="tsaC"
                     /locus_tag="OQL90_001548"
     CDS             22627..23199
                     /gene="tsaC"
                     /locus_tag="OQL90_001548"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1368369.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23204..24022
                     /gene="aroE"
                     /locus_tag="OQL90_001549"
     CDS             23204..24022
                     /gene="aroE"
                     /locus_tag="OQL90_001549"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1368370.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24019..24276
                     /locus_tag="OQL90_001550"
     CDS             24019..24276
                     /locus_tag="OQL90_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1368371.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24252..24806)
                     /locus_tag="OQL90_001551"
     CDS             complement(24252..24806)
                     /locus_tag="OQL90_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1368372.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6418 a         5974 c         6544 g         6195 t
ORIGIN      
        1 gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc cgcaagcgaa
       61 tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg cgccaagggc
      121 ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta tgcgataccc
      181 ccagtaaatg acagcgaatg tgccgtaacc aacaattgtc ccccataacg catcggctaa
      241 accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg ggcacgtaaa
      301 tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg ataaacaagc
      361 gtacaaaatc agaaagggaa gggtagtgtc cataaaacat cctctgtaat tggagtatca
      421 ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta acctgattcc
      481 ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc gcaatctgcg
      541 cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta taatgcgcgg
      601 gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt tggctaccta
      661 acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc aatcgtaatg
      721 ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct catgcagaac
      781 caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca agcaaccgcg
      841 gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat cccgctgccg
      901 acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga cgcgcgcgat
      961 cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac cgagaaaacc
     1021 gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat cagcctgggt
     1081 taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc ggtaaaaaag
     1141 tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc gtaatcgaag
     1201 ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac cgtgctattc
     1261 aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct ggccacttcg
     1321 ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct gaaggcgaag
     1381 agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa aaagttgacg
     1441 taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg aacttccgta
     1501 cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct atcggtcaga
     1561 accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg ggtaacgaac
     1621 gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac ctgctgctgg
     1681 ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca gctgtgaagg
     1741 cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct gactgtttcc
     1801 gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt tgttgcttat
     1861 gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt aactggttcc
     1921 ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc tatcaagagc
     1981 ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca cagtcaaaaa
     2041 gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact ggtacgtcag
     2101 gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa gctgctggca
     2161 cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga gctggacgaa
     2221 aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc aactggtatc
     2281 gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc tgttaagcaa
     2341 gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg cgtgcaccgc
     2401 acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta ctcaaagttg
     2461 ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt gaagtcgaag
     2521 tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga cagcgtatcg
     2581 gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag aatctggact
     2641 tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta aatgtaaacc
     2701 gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc acaagggcaa
     2761 accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca acaatggccg
     2821 tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg ttgacttcaa
     2881 acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc cgaaccgttc
     2941 cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc tggcccctaa
     3001 aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca aaccaggtaa
     3061 caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag aaatgaaacc
     3121 aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg ttgctcgtga
     3181 tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag aagcagactg
     3241 ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc tgggtaaagc
     3301 aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga tgaacccggt
     3361 agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg taactccgtg
     3421 gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata aattcatcgt
     3481 acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc aagaaaggtc
     3541 cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc ggagacaaga
     3601 agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc ggtttgacca
     3661 tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa atggtcggtc
     3721 acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct gataaaaaag
     3781 cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc gccatgctcg
     3841 ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga aagtgtcgca
     3901 ggctctggat attttgacct acaccaacaa gaaagcggct gtactggtca agaaagttct
     3961 ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg atctgaaagt
     4021 tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc gtgcaaaagg
     4081 tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt ccgatcgctg
     4141 agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg cctgggtatt
     4201 gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga caacctggac
     4261 agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc cgtatctcgt
     4321 atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc tcgcccgggt
     4381 atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt agcggacatc
     4441 gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact ggacgcaaaa
     4501 ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg tcgtgctatg
     4561 aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt tgaagttagc
     4621 ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg tcgcgtaccg
     4681 ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac cacttacggt
     4741 gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat ggctgctgtt
     4801 gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg ccgtaaataa
     4861 ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca caaaggccgt
     4921 aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct gaaagctgtt
     4981 ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat gacccgtgca
     5041 gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat cactgaaaag
     5101 ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt tgccttgatt
     5161 cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc ccgtgaagca
     5221 ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa gacggtgatg
     5281 taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc gagctgctga
     5341 acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag ctgcaacagt
     5401 ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta ctgaacgaga
     5461 aggctggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg ttagcgacaa
     5521 aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga tctacggtaa
     5581 attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat gcggtatcgg
     5641 tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct ggacgctggt
     5701 tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcgatacg aataaacggc
     5761 tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta taatgccgcg
     5821 ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag ttgacattag
     5881 cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa ctccggtgca
     5941 cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc aggcgtaggc
     6001 gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa aaaaggtgat
     6061 gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga cggttctgtc
     6121 attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca gcctatcggt
     6181 acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat gaaaattatc
     6241 tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc gtcgtgatga
     6301 cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta agaatgtcct
     6361 gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc agaagccggt
     6421 tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc aggtttccaa
     6481 cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta gattcgaaga
     6541 cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat ttggagtagt
     6601 acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact catgactgag
     6661 tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct gaacatgggt
     6721 gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga cctggcagca
     6781 atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg cttcaaaatc
     6841 cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat gtgggagttc
     6901 tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg cctgtccgct
     6961 aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat catcttccca
     7021 gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat taccactact
     7081 gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc gttccgcaag
     7141 taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta aaacgcgtag
     7201 ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc tctgatgtga
     7261 acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg ccgcgtgatt
     7321 ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat ggtttcctgc
     7381 ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt gaaatcccgg
     7441 gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa agacagatga
     7501 gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag gccgcgaaca
     7561 aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac gtgctgaagg
     7621 aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa ctggaactta
     7681 ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc agccgcccag
     7741 gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt ctgggtatcg
     7801 cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag gctggtcttg
     7861 gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt gttgctaaag
     7921 caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt attacgatca
     7981 aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt aaacatgcag
     8041 ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca caggctggta
     8101 ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc actaagaagc
     8161 tgcagctggt tggtgtaggt taccgtgcag cggttaaagg taatgtgatt aacctgtctc
     8221 tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct gaatgtccga
     8281 ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag gttgcagcgg
     8341 atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt tacgccgacg
     8401 aagtcgtgcg tatcaaagag gctaagaaga agtaaggtaa cactatggat aagaaatctg
     8461 ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc gcaactcgcc
     8521 tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg aacggttctg
     8581 aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg aagtacaccg
     8641 gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct ctggaaaaag
     8701 gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt gtccaggcac
     8761 tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta agatggctca
     8821 catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc gcgtatctaa
     8881 aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg gcgatggtaa
     8941 cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga tccagaaagc
     9001 gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca ctctgcaaca
     9061 ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt ccgaaggtac
     9121 cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg ttcataacgt
     9181 tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa ctattgatgg
     9241 cctggaaaat atgaactctc cagaaatggt cgctgccaag cgtggtaaat ccgttgaaga
     9301 aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc gcagtgcaat
     9361 cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc gtattggtca
     9421 caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg tttccttcat
     9481 ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg aaggctccaa
     9541 aaaggcgggt aaacgcttgg gtcgtggtat cggttctggc ctcggtaaaa ccggtggtcg
     9601 tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt tcgagggtgg
     9661 tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta aagcagcgat
     9721 tacagccgaa gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg tagacctgaa
     9781 cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag tgatcctggc
     9841 tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag gcgctcgtgc
     9901 tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct aaacaaccgg
     9961 gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga ctgctgtttg
    10021 ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct ggtattgatg
    10081 ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag atgtttaaca
    10141 tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc atgccgtata
    10201 tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg gcggaaatta
    10261 agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgctac ggtactctgg
    10321 tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg cctggtatgc
    10381 aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta agtctggtca
    10441 caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt atcggcaacg
    10501 gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc attgcccata
    10561 ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg gttgcagtat
    10621 tagtatttgc agtgacgttc tttgttgtat ttgttgagcg tggtcaacgc cgcattgtgg
    10681 taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc acacatttac
    10741 cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt attattctgt
    10801 tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg ctgacaacaa
    10861 tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg tctgcaatca
    10921 tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca gcagataacc
    10981 tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg gcgaagtata
    11041 tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct gtatattacc tttatctgcc
    11101 tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt gggacctcac
    11161 tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact ctgatgatgt
    11221 ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc cgataattgg
    11281 tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcaa gaaattatgc
    11341 cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag tgccgagccg
    11401 aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag ttgggttgag
    11461 ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg agtatcctga
    11521 aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata gtggcccgta
    11581 tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact tcgatttatg
    11641 gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct gaagatgtta
    11701 agatcagtga gctgtctgaa ggacaaatcg acacgctgcg tgacgaagtt gccaaatttg
    11761 tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg atggatcttg
    11821 gttgctatcg cggcttgcgt catcgtcgtg gtctcccggt tcgcggtcag cgtaccaaga
    11881 ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg gggtgattga
    11941 ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt ctctgacggc
    12001 gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga tcgtcagggt
    12061 aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg caaatccact
    12121 ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga atacggcatc
    12181 aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac tattcgtgct
    12241 ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat ccctcataac
    12301 ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt ttgttggaga
    12361 aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga gggcaccgac
    12421 ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga acaagctcct
    12481 ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt gcgtgaaaag
    12541 caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta ctacaaagaa
    12601 gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga aggtcgtctg
    12661 gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg tcagctggtt
    12721 agccataaag caattatggt aaacggtcgt gttgttaata tcgcttctta tcaggttagt
    12781 ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt gaaagccgct
    12841 ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc tggcaagatg
    12901 gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat taacgaacac
    12961 ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca caatgcaggg
    13021 ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga gttcgacgca
    13081 cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg gtaacgcact
    13141 gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg agattgatgg
    13201 tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg aaatcctgct
    13261 caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc ttaccttgaa
    13321 taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg atgtcgaaat
    13381 cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta ttagcatgcg
    13441 tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc attcggaaga
    13501 agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg tggagcgtat
    13561 tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca agctggtcat
    13621 cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg cggcaaccat
    13681 tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc ctgaagtgaa
    13741 agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc tggaattgac
    13801 tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg atctggtaca
    13861 acgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc ttactgagat
    13921 taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcctggaaa actggccacc
    13981 ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag aaggataagg
    14041 tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat cgccaggcta
    14101 tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg actctgccta
    14161 aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag actgatagcg
    14221 ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg gcaaaactgt
    14281 ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt attctgaagt
    14341 gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt gatcgttcag
    14401 agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg ccccggcggg
    14461 tttttttata cccgtagcat ccccacttat ctacaatagc tgtactcttt ttgttcatcc
    14521 cctggagtat ttatgtggtt acttgaccag tgggcagagc gtcatatagc agaagcgcaa
    14581 gcgaaaggtg agtttgataa tctggcaggt agcggcgaac cattgatact ggatgatgat
    14641 tctcatgtgc caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc tggttgctta
    14701 ccgccagaac ttgagcagcg gagggaagca attcagcttc tggatatcct gagaggtatc
    14761 cgtcacgatg atccgcaata tcaagaggtt agccgtcgat tgtcattact ggaattgaag
    14821 ctgcgacaag ctggatttag taccgatttt ttacgcggcg attatgctga caagttgttg
    14881 gacaaaatca acgataacta gtggagtatg tatgtatcgc attggtgagc tggcaaaaat
    14941 ggcggaagta acacctgaca cgattcgtta ttacgaaaaa cagcagatga tggagcatga
    15001 agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc gatctccagc gattgaaatt
    15061 tatccgccat gccagacaac taggtttcag tctggagtcg atccgcgagt tgctgtcgat
    15121 ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc aggaaagatt
    15181 gcaggaagtc gaagcacgga tagccgagtt gcagagtatg cagcgttcct tgcaacgcct
    15241 taacgatgcc tgttgtggga ccgcccatag cagtgtttat tgttcgattc ttgaagctct
    15301 tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt tttgcactgg caggattaca
    15361 ctcgcgccgt aaaataacca attggagttt ttatgagccg atatcagcat actaaagggc
    15421 agataaggga taatgcgata gaagcattac tacatgatcc cttattccga cagcgcgtag
    15481 agaaaaataa gaaggggaaa ggcagttaca tgcgaaaagg aaaacatggc aatcggggga
    15541 actgggaggc cagtggcaag aaagtaaatc acttttttac cactggtctt ctgctttcag
    15601 gcgcttgtta agagcggtta ttctgctctt tcagcaaatc acgaatttct gccagtaata
    15661 cttcttcttt agttggtgca ggtgcggcag ctggttcttc ttttttccga ttcagtttgt
    15721 tgattagctt aatcgccata aagatggcaa aggccacaat cagaaaatca aagacgtttt
    15781 gaatgaagac accgtaatgc atcacaacag cagggatatc cccctgcgca tcgcgtagcg
    15841 tgacagcaaa ctgtttaaag tcgatcccgc caattaataa gcccagaggt ggcatgatga
    15901 tatcggcaac cagtgaagat acaatcttcc cgaatgccgc accgataatg acacccaccg
    15961 ccaaatccac cacgttcccg cgcatcgcaa attcgcgaaa ttctttaata atgctcatgt
    16021 tattctccct atgaagccga caaccataag tctaacaaat gttaagccat tttcctgcca
    16081 ggaatgaatt taattaatcc ttgataatca aagggaaata ataaggcgtc attagacgcc
    16141 ttattaatta caagaagaaa gggcttggct ggaagagtct ttcgacgtcg gtaataaact
    16201 ttttatctgt gaggaacata attacgtgat cgccttgctc aatgcgcaga ttgtcattgg
    16261 caatcatcac gtcgtttcca cgtaccaccg ctccaataat cgttcctggc ggtagcttga
    16321 tttcgtcaat gactctgccg acaacgcgtg aggtgctttc atcaccgtga gcaacggctt
    16381 caatagcttc tgctacgccg cggcgcaatg aggaaacacc aacaatatct gcttttcgca
    16441 catggctaag caacgcagaa atagttgctt gttgtggtga aattgcaata tcgatcacgc
    16501 tcccctgaac cagatccaca taagcgcgac gctggatcaa taccatcacc tttttcgcac
    16561 ccatacgttt ggcaagcatg gcggacatga tattggcctc gtcatcgttg gtgacggcaa
    16621 taaacagatc aacttgatcg atatgttctt cggccagcag ttcttgatcc gacgcatcac
    16681 caaaaaagac gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc tgctgattac
    16741 gttcgatgag tttaacgctg taatcttttt ccagacgacg cgccagccct gcaccgatat
    16801 taccgccacc aaccagcatg atccgcttat acggtttttc cagtcgctgt aattcactca
    16861 tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac ttcatcacca gcttcaacaa
    16921 tggtcgaacc ttgtggacga atggggcgat cgtggcggaa aattgctgcc acacgagtat
    16981 cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt accaatcagc gggccgccat
    17041 aataggcttt aactacagcg aggctgactt taccctcagc gaagttcacc acctgcaatg
    17101 cgcctggata ctcaatcagt cggtaaatat tatcgataac caactgctct ggtgcgatca
    17161 gatgatcaat cggtacagca tctgaatgaa atagcttatc ggcatcgcgt acgtagtctg
    17221 gtgagcggat gcgagcgatg cgattagggg tgttgaaaag tgagtaggct acctggcagg
    17281 caaccatatt ggtttcatct gaactggtta cagcaaccag catatcggcg tcgtcggcac
    17341 ctgcctcccg caatacgcgt ggatgagagc catgcccctg cacgactcgc aggtcaaatt
    17401 tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac aacagtaata tcgttgttct
    17461 cgccaaccag gttttccgcc agtgtgccgc caacctggcc ggcacccaga atgataattt
    17521 tcatcagtct cgacccgtta tctcatcact ttttgattag cttagcgtaa aagaagccgt
    17581 cgccctcttc ggcaccaggc agattttgtt tacccggttg ctctggtgtt cctgtttcgc
    17641 aaagttcggc atcagcggta cgttgcaaaa aagctttaat ctgcaggcta ttctcttccg
    17701 gtaacaccga acaggtggca tagaccaggg ttccaccgga ttttaaatgc ggccaaatgg
    17761 cgtcgagaat ttcagactgc aattgcgcga gttccgggat atcgcgatcg cgacgtaacc
    17821 atttaatatc cggatggtga cgaatcaccc ccgttgctga acaaggcgca tctaataaaa
    17881 tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta acggccatca ccttgtttca
    17941 cggttgcctt cataccaagg cgttttaaat tgtcgtaaac gcgagagagg cgctgttcgt
    18001 caatatcaac cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc gttgttttac
    18061 cgccgggggc ggcacaaaga tccagaatgt gttcaccgtt ttgtggcgca agccaggtca
    18121 tgcaaccttg tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa ccaggtagcg
    18181 catgaacagg tgcaggtgtt tccagacgta cagcatcagg gtaatccgca tgcgggaaac
    18241 ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga tgcgtacgat
    18301 taacgcgcag ccacattggc ggacgctggt tattggcttc gacgatggat tgccactgct
    18361 ctggatacgc tttttgcaga cgcttcagca accaggaagg atgcagataa cgtgcatcac
    18421 tggcattaaa cttggctaat aactcttctt gctgacgctg gaactggcgt aatacaccgt
    18481 taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc gtttcagcca
    18541 gcgcagcatg aggtggaatg cgggtataaa gcagttgata caaaccaacc ataatcaggt
    18601 aatgcacagt gcgctgtttg ccggtcatcg gacgggccat taacttatta atcagccagt
    18661 ctaactgcga aagcgtacgc agtacgccaa agcacaactc ttgaagaagt gctttgtctt
    18721 tatcagaaac tttttgctgg agcggtggca gaatgttgct taatgattgc ccttgctcga
    18781 cgacttgttc aacggcctgg gccgccatgc tacgtaaatt acgttgtttt ttcataagta
    18841 taaaaatgcc cggtaagacc gggcttagaa gagtggacta tcaggccaga cggttgcccg
    18901 gaacaaacca ttcccgacga gaattcagga ggtcttgcgc gctcatcgct ttcttacccg
    18961 caggttgtaa cgagagcaga ttcaggattc catcaccagt tgcaacctga atgccttgtt
    19021 tgttggcttc aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg accgacgctt
    19081 tccagacttt aaccggctgt ctttcaattt ccagccagct cattggccat ggattgaaag
    19141 cgcgaatgta gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc gcttcttctt
    19201 tactcaactt ttcggcgtaa gtgacaagag tttcgtcctg aacttctggt ttcgccgtgc
    19261 cgtctgccag ttgtttcaac gtggtgataa gcccttgtgg gccaagctct gccagcttgt
    19321 cgtacagcgt accactggta tcttctgcag taatcgggca ggagagctta tagagcatgt
    19381 caccggtgtc taaaccgaca tccatttgca taatggtcac accagtttct gcatcacccg
    19441 cccatagtga gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt gaaccatgaa
    19501 cgttgataca gccaagacgc ggcatctcca gcactgcttt aggcagaatt aagccatagg
    19561 cgacgacgac cataacatca gcctgcagat cggcgaccag ttgctggttt tcttgtggac
    19621 gcagggaaac aggttgaaaa acgggcagac ctttttcctc agccagaact ttaaccgggc
    19681 tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac acgccaacga
    19741 cgttatgacc agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca ggtgtacccg
    19801 caaaaataat acgtagtgat tctgacacgt tagttcttat ccttaagccc gggctttcag
    19861 acgatccagt ttttcaactt tctgacgaat acgttgttgt ttcaacggtg acagataatc
    19921 cataaacagt ttgccgacca ggtgatccat ctcatgctga atacagatgg ctaacagacc
    19981 gtctgcttcc aattcaaatg gtttaccgtc gcggtccagg gcgcgaattt taactttctc
    20041 tgcgcgtggc actaaagcac gttgttcagg gatcgacagg caaccttctt caatgcctgt
    20101 ttcgccgctt ttttctaaaa gctccggatt gattaacacc agccgttcgt cacggttttc
    20161 cgaaacatca ataacaatga tacgttgatg gatatcaacc tgggttgccg ccaggccaat
    20221 accttcttct gcgtacatcg tctcgaacat atcatcgacg atacgctgaa tttctgcatt
    20281 cacttcttct accggtttag caactttgcg aagccgctcg tccggaatat gtaacacttg
    20341 caaaactgac ataaatctcc agagatgtgt tcaggagtta gaaagattat ttcttctatt
    20401 ctagacaaat ccccctctga ttgacagcat cactgaccaa tcgcaaagat tgctaaggct
    20461 gcttatggca gggagataag gatggtcgat acagatattt ggctgcgttt aatgagcatc
    20521 agcagcttgt acggcgatga tatggtccgt atagctcact ggctggcaaa acagtcgcat
    20581 attgatgcgg ttgtattgca gcaaacaggg cttacattgc ggcaggcaca acgctttctt
    20641 tcatttccgc gaaagagtat cgaaagctca ctttgttggt tggagcaacc caaccatcat
    20701 ttaatccctg cggacagcga attttatcct cctcaacttc tggcgacgac agattacccc
    20761 ggcgcactgt ttgttgaagg agaactgtac gcgctgcatt catttcagct tgccgtagtg
    20821 gggagtcggg cgcattcatg gtatggcgag cgatggggac gattattttg cgaaactctg
    20881 gccacgcgtg gagtgacaat tacgagtgga ctggcgcgtg gaatcgatgg tgtggcgcat
    20941 aaagcggcct tacaggtaaa tggcgtcagc attgctgtat tggggaacgg acttaatacc
    21001 attcatccgc gccgccatgc ccgactggct accagtttgc ttgaacatgg tggggcactt
    21061 gtctcggaat ttcccctcga tgtttcaccc cttgcttaca atttcccacg aagaaatcgc
    21121 attatcagtg gtctaagtaa aggtgtactg gtggtggaag cggctttgcg cagtggttcg
    21181 ttggtgacag cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt gccaggacca
    21241 atagggaatc cgggaagcga agggcctcac tggttaataa aacaaggtgc gattcttgtg
    21301 acggaaccgg aagaaattct ggaaaacttg caatttggat tgcactggtt gccagacgcc
    21361 cctgaaaatt cattttattc accagatcag caagacgtgg cattgccatt tcctgagctc
    21421 ctggctaacg taggagatga ggtaacacct gttgacgtcg tcgctgaacg tgccggccaa
    21481 cctgtgccag aggtagttac tcaactactc gaactggagt tagcaggatg gatcgcagct
    21541 gtacccggcg gctatgtccg attgaggagg gcatgccatg ttcgacgtac taatgtattt
    21601 gtttgaaacc tatattcaca cagaagctga gttgcgtgtg gatcaagaca aacttgaaca
    21661 ggatcttacc gacgcaggat ttgatcgaga agatatctac aatgccctgc tttggctgga
    21721 aaagcttgcg gattatcagg aaggactgac agaaccgatg caactggcct ctgatccact
    21781 ctccatgcgt atttataccc ctgaggagtg tgaaagactg gatgccagct gtcgtggttt
    21841 tctgcttttc cttgagcaaa ttcaggtgct taaccttgaa atccgtgaaa tggtgataga
    21901 gcgagtgctc gcgctggata ccgcagagtt cgacctggaa gacctgaaat gggtaatcct
    21961 gatggtgttg ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg aagaattact
    22021 ctttgaagtg aatgaaggta tgctgcatta atcatttctt taattcagca taagttgtta
    22081 tggcgaaatc agcactgttc acggtgcgta ataatgagtc ctgcccaaag tgcggggctg
    22141 aactggttat tcgatccggg aaacacggtc cgtttcttgg atgctcacag tatccggcgt
    22201 gtgactacgt ccgtcctctg aaatcttcag cggatggaca tattgtcaaa gttctggagg
    22261 ggcaggtttg ccctgcatgt ggcgcaaatc tggtattacg ccagggacgc tttggtatgt
    22321 ttattggttg cagtaactac cctgaatgcg aacataccga acttatcgat aaaccggacg
    22381 aaacagcaat tacatgcccc caatgtcgga cgggccatct ggtccagcgc cgctcccgtt
    22441 atggcaaaac atttcactcc tgtgatcgct acccggagtg tcaatttgcc attaacttca
    22501 aacccatagc tggagaatgc cctgagtgtc attatccact actcatcgaa aagaaaaccg
    22561 cgcagggtgt aaaacacttt tgtgccagta aacaatgtgg aaagccggtt tcggcggaat
    22621 aataacgtga ataataacct gcaaggagac gctatcgcag ctgcgataga tgtcctcaat
    22681 gaagaacgtg tcatcgccta tccaacggaa gccgttttcg gtgttgggtg cgatcctgat
    22741 agcgaaacag cagtgatgag actgttggag ttaaaacagc gtccggttga taaggggctg
    22801 attttaatcg cggcaaatta cgagcagctt aaaccctata ttgatgacac catgctgact
    22861 gatgcgcagc gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt tgtctttccc
    22921 gcgcctgcga caacaccgcg ctggttgacg ggccgctttg attcgcttgc tgtacgagtc
    22981 accgaccatc cgttggtggt tgctttgtgc caggcttatg gtaaaccgct ggtttctacc
    23041 agtgccaact tgagtggatt gccaccttgt cgaacagtag acgaagttcg cgcacaattt
    23101 ggcgcggcgt tcccggttgt gcttggtgaa acgggggggc gtttaaatcc ttcagaaatc
    23161 cgcgatgccc tgacgggtga actgtttcga caggggtaac ataatggaaa cctatgctgt
    23221 ttttggtaat ccgatagccc acagcaaatc gccattcatt catcagcaat ttgctcagca
    23281 actgaatatt gaacatccct atgggcgcgt gttggcaccc atcaatgatt tcatcaacac
    23341 actgaacgct ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac cttttaaaga
    23401 agaggctttt gccagagcgg atgagcttac tgaacgggca gcgttggctg gtgctgttaa
    23461 taccctcaag cggttagaag atggacgctt gcagggtgac aataccgatg gtataggttt
    23521 gttaagcgat ctggaacgtc tgtcttttat ccgtccgggt ttacgtattc tgcttatcgg
    23581 cgctggtgga gcatctcgcg gcgtactact accactcctt tccctggact gtgcggtgac
    23641 aatcactaat cggacggtat cccgtgcgga agagttggct aaattgtttg cgcacactgg
    23701 cagtattcag gcgttgggta tggacgaact ggaaggtcat gagtttgatc tcattattaa
    23761 tgcaacatcc agtggcatca gtggtgatat tccggcgatc ccgtcatcgc tcattcatct
    23821 aggcatttat tgctatgaca tgttctatca gaaaggaaaa actccttttc tggcatggtg
    23881 tgagcagcga ggctcaaagc gtaatgctga tggtttagga atgctggtgg cacaggcggc
    23941 tcatgccttt cttctctggc acggtgttct gcctgacgta gaaccagtta taaagcaatt
    24001 gcaggaggaa ttgtccgcgt gaatcaggcc atccagtttc cggacaggga agagtgggac
    24061 gagaataaaa aatgtgtatg ttttcccgct ctcgtgaatg gtatgcaact gacatgcgcg
    24121 atctctggcg agagtctggc gtatcgcttt actggagata cgccagaaca gtggttagcg
    24181 agttttcgtc agcatcgctg ggacctggaa gaagaagcgg aaaacttaat tcaggaacaa
    24241 agtgaagatg atcaaggctg ggtctggtta ccctgatcca gatattcgtc cttccatttc
    24301 acgtaattat tcgcggaata gcgtaaccca gccttctctt catcacttaa cgggcggatc
    24361 tgtttgacgg ggctaccgag atacagatat ccgctctcca gccgtttatt ttgtgggacc
    24421 agactacccg caccaatcat cacatcatct tctactattg cgccatcaag taaaattgag
    24481 cccatcccaa ccaaaactcg attgccaatg gtgcagccgt ggagcatcac cttgtgacca
    24541 acagtgacat cttcgccaat ggttaatggg ttgccatctg ggttgtacga ggatttatga
    24601 gtgacatgca acatactgcc atcctggata ttggtgcgtg ctccgatctg tacataatgt
    24661 acatctccac gaatcacaac gagcggccag atccccacat catcagccag acgaacgcca
    24721 ccaatcacga cactgctatc gtcgatcatt acgcgctgac cgatttgtgg aaaaagatcg
    24781 cggtatgggc gtaaaacatc agacatactt acctcagcaa taaatgattt actaatgact
    24841 ttgggggcat tattggcctt gtgcaagtct tttagtatgc aaaaaagcac cgttttgtgt
    24901 gcgattgcag caaaaagggt gaaaaaacaa caaacagaaa aaaagatcaa aaaaatactt
    24961 gtgcaaaata ttgggatccc tataatgcgc ctccgttaag acgacaacgt gaaacacttc
    25021 acaggatggt cggaacaacg aagagaaaaa atcctgaaat tcagggttga ctctgaaaga
    25081 ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa agccgcgtcg c
//
LOCUS       DAJVOY010000042        25128 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVOY010000042 DAJVOY010000000
VERSION     DAJVOY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.042"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..648
                     /gene="yaaA"
                     /locus_tag="OQL90_001552"
     CDS             <1..648
                     /gene="yaaA"
                     /locus_tag="OQL90_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1368373.1"
                     /translation="PQISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDV
                     YTGLQAETFSEDDFDFAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQ
                     FWGDIITNKLNEALAAQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKI
                     ISFYAKKARGLMSRFIIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(801..1097)
                     /locus_tag="OQL90_001553"
     CDS             complement(801..1097)
                     /locus_tag="OQL90_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1368374.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1311..2597)
                     /gene="thrC"
                     /locus_tag="OQL90_001554"
     CDS             complement(1311..2597)
                     /gene="thrC"
                     /locus_tag="OQL90_001554"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1368375.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLCLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2598..3530)
                     /gene="thrB"
                     /locus_tag="OQL90_001555"
     CDS             complement(2598..3530)
                     /gene="thrB"
                     /locus_tag="OQL90_001555"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1368376.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3532..5994)
                     /gene="thrA"
                     /locus_tag="OQL90_001556"
     CDS             complement(3532..5994)
                     /gene="thrA"
                     /locus_tag="OQL90_001556"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1368377.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFVQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6019..6138)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6074..6139)
                     /gene="thrL"
                     /locus_tag="OQL90_001557"
     CDS             complement(6074..6139)
                     /gene="thrL"
                     /locus_tag="OQL90_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1368378.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6353..7039)
                     /locus_tag="OQL90_001558"
     CDS             complement(6353..7039)
                     /locus_tag="OQL90_001558"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1368379.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7439..7579)
                     /locus_tag="OQL90_001559"
     CDS             complement(7439..7579)
                     /locus_tag="OQL90_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368380.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            7675..8391
                     /gene="arcA"
                     /locus_tag="OQL90_001560"
     CDS             7675..8391
                     /gene="arcA"
                     /locus_tag="OQL90_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1368381.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8451..9803)
                     /gene="creD"
                     /locus_tag="OQL90_001561"
     CDS             complement(8451..9803)
                     /gene="creD"
                     /locus_tag="OQL90_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1368382.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9861..11285)
                     /gene="creC"
                     /locus_tag="OQL90_001562"
     CDS             complement(9861..11285)
                     /gene="creC"
                     /locus_tag="OQL90_001562"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1368383.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11285..11974)
                     /gene="creB"
                     /locus_tag="OQL90_001563"
     CDS             complement(11285..11974)
                     /gene="creB"
                     /locus_tag="OQL90_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1368384.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11987..12460)
                     /gene="creA"
                     /locus_tag="OQL90_001564"
     CDS             complement(11987..12460)
                     /gene="creA"
                     /locus_tag="OQL90_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1368385.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            12671..13540
                     /gene="robA"
                     /locus_tag="OQL90_001565"
     CDS             12671..13540
                     /gene="robA"
                     /locus_tag="OQL90_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1368386.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13537..14184)
                     /gene="gpmB"
                     /locus_tag="OQL90_001566"
     CDS             complement(13537..14184)
                     /gene="gpmB"
                     /locus_tag="OQL90_001566"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1368387.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14236..14757
                     /gene="yjjX"
                     /locus_tag="OQL90_001567"
     CDS             14236..14757
                     /gene="yjjX"
                     /locus_tag="OQL90_001567"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCS1368388.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14842..15168)
                     /gene="trpR"
                     /locus_tag="OQL90_001568"
     CDS             complement(14842..15168)
                     /gene="trpR"
                     /locus_tag="OQL90_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1368389.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15258..17195)
                     /gene="sltY"
                     /locus_tag="OQL90_001569"
     CDS             complement(15258..17195)
                     /gene="sltY"
                     /locus_tag="OQL90_001569"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1368390.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17406..19073
                     /gene="ettA"
                     /locus_tag="OQL90_001570"
     CDS             17406..19073
                     /gene="ettA"
                     /locus_tag="OQL90_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1368391.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19380..20612)
                     /gene="nadR"
                     /locus_tag="OQL90_001571"
     CDS             complement(19380..20612)
                     /gene="nadR"
                     /locus_tag="OQL90_001571"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1368392.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(20633..22015)
                     /gene="radA"
                     /locus_tag="OQL90_001572"
     CDS             complement(20633..22015)
                     /gene="radA"
                     /locus_tag="OQL90_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1368393.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22064..23032)
                     /gene="serB"
                     /locus_tag="OQL90_001573"
     CDS             complement(22064..23032)
                     /gene="serB"
                     /locus_tag="OQL90_001573"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1368394.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23138..23782
                     /locus_tag="OQL90_001574"
     CDS             23138..23782
                     /locus_tag="OQL90_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1368395.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23810..24826
                     /gene="lplA"
                     /locus_tag="OQL90_001575"
     CDS             23810..24826
                     /gene="lplA"
                     /locus_tag="OQL90_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCS1368396.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            24858..25007
                     /locus_tag="OQL90_001576"
     CDS             24858..25007
                     /locus_tag="OQL90_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368397.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
BASE COUNT         6051 a         6925 c         6247 g         5905 t
ORIGIN      
        1 ccgcagatta gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc
       61 tttcatgact ggcagccaga tttcacgccg gcgaatgccc gccaggcgat tctggcgttt
      121 aaaggtgatg tctacaccgg cttgcaggcc gaaaccttca gcgaagacga tttcgatttt
      181 gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc gctcgattta
      241 atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg gaaagatctg
      301 tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc agcacaaggc
      361 gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa
      421 ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa atttaagatc
      481 atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg
      541 ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt tgatgaagct
      601 tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt ttgcagtcaa
      661 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt caatatattg
      721 cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg aatgccggat
      781 tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc gccgtgatga
      841 tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg gtgccagcga
      901 ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg acctccatcc
      961 cagtaatagc cacgattatc acgattgcct atctgtaatt ttactgacgg gactaacgta
     1021 atttccgcag cctgtgctgc catgggagcg accagaacca gggaaagtgc gagtacgata
     1081 gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg
     1141 agattacgcg atgagaacga cggttgttat tctccgcaat cctaatctct aagttaccgc
     1201 atttattgag aatatctcct ttttccctgt cgtttttctc cataatttct tcataaaaaa
     1261 gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatt ttactgatga
     1321 ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgtga aagcaagggt
     1381 aaatcagcac gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc
     1441 acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga
     1501 ttcaactggt cacgcagcgc acgatacgct acggcagcgt gcggctccga ggtgtagccc
     1561 agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc tgcataaccc
     1621 agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttc
     1681 ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg tgaccactga
     1741 ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc
     1801 accggcagac agagtgactt cgccagcaga cccgccgtca aatcgccgaa gtttccgctt
     1861 ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac tgcttcaaag
     1921 tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagctga gtttaacccc
     1981 agcgccactt tcagctcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg
     2041 aaatcgccgt cgatggcaac agtttcgata ttgccgccca atgtacagaa cagtttttct
     2101 tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt cggtaaaccg
     2161 tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag aatggtcact
     2221 ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta
     2281 aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc aacattggcg
     2341 accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc ctgcgggatt
     2401 tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc
     2461 atctcatcaa tttcagtcag gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga
     2521 tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcatt gtgatctttc
     2581 agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa
     2641 atatgaacaa aaccttcctg attttgcaga tagttcttac ccaaccagtc ggcaacgcgc
     2701 tgggcggtat ccggcttgtc acacagagcg aacaaggtcg ggccggagcc ggagataccg
     2761 ctcgctaccg cgccgatttc cgcaaccgcc tgccgcgcct gccggaagcc aggcagtaac
     2821 cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga
     2881 cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg
     2941 cggcgatact gcgccggtaa aatagccctg gcttctgccg ttgagacttt aatccccgga
     3001 tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat gatgtcgttt
     3061 tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc gtaatgaatg
     3121 ctgccggaga ttcgtccttc cagctcgccc atcaaagcca gcaaacgagt gtcattaagc
     3181 ggcttgccac agtgttcatt catcgccatc agcgccgcga ccaccgaaca ggcgctggag
     3241 cctaagcccg aaccgattgg catattcttt tccagagtca tcgccactgg aatttgcttg
     3301 ccaagctcct ggcaaaaacg ctcccagcac tgataaacga tattttcccg tggttctgac
     3361 ggcagcttat cggcaaagcg tccgaggttg ttgagactga atgtctctgc cgcctcaacc
     3421 gtgactacat ctccgagcaa tgcaccatca acaggtgtca ccgccgcccc gagcacatca
     3481 aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc
     3541 taacttccat gagagggtac gtagcagatc ggcaaagaca ccggcagctg taacgtcatt
     3601 gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc tataaaaggc
     3661 cagggcgttt tcgccatttt tcactttgaa cagcggatca ttaccatcca cttcggcaat
     3721 cttcacgcgg caggcaccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc
     3781 atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat
     3841 aaaagcggca acatcaccct cagcgttaaa ctctgcgggc agcacaggtt caatttcaat
     3901 atccgccagc tccagttcac gtcccgtttc acgagcgaga atcaatagct tacgcgctac
     3961 atccatacca gaaagatcat ctcgcggatc cggttcggta taacccattt cccgcgccag
     4021 agtagtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga
     4081 acctgaaaga atgccggaga acttcatcaa ttcatcacca gcattgagca gattttgcag
     4141 gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga
     4201 tttttccgcc gcatgacgca acagatggta gtaatccatc gacgaggtgt tggccttttt
     4261 gttcggcgtg acaacgtgga aaccttcgcg caagaagtcg gcatattgat ccgccactgc
     4321 ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg
     4381 aattaagcgc ccgagattaa acggctcttt ggcttgcgcc agttcttcct gccagttttc
     4441 cagatttagg ccatgcacat tggtgagcag tgccttcgag ttggcaacac cgcagacacg
     4501 taagtcgata tgtttattct tcagccagct ttgttgacgc ttcagttgct ccagcagcgc
     4561 accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag
     4621 catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat
     4681 tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagcgc
     4741 ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat
     4801 aatggccagc cgttccgtca ccgccagcgg ctccagtaag ccttctttca gttccaggta
     4861 gaactcttcc tgcattgccc gttcagctcg cacacagtcg ctttgtggaa cgcagaaact
     4921 gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat
     4981 tgcagcaaag acgcgcgccg ccatgccgac cattcctttc atccccggac cagaaacgct
     5041 gaacattgcc atattattca gattggaaat gcctttgacc ggtaattcgt cttcatcacg
     5101 gctggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg
     5161 gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag cgccgaagta
     5221 ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg
     5281 acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg
     5341 taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttacgtccaa gtaccaccag
     5401 ttcgcctttt tcattaccgg aggtgaaacc tgccatcaac accatgtgat cagccggaat
     5461 gcggcttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt cgaggtaatg
     5521 ccccactgcc agcagttttt cgaccggatc gataacggta acgttgtgac cacgagcttc
     5581 taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt
     5641 gatgctatcc gggcactgcc ccaacaaact aatgccatgc aggacatgtt ttatttggac
     5701 aaattcctga tcgacgaaag ttttcaattg cgccagcggg aaccccggct gggcggcggc
     5761 gagtcccgtc aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc
     5821 ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt tggcgggggc
     5881 agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacacg
     5941 cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt
     6001 tacctcgtta cctttggtcg aaaaaaagcc cgcactgtca ggtgcgggct tttttctgtg
     6061 tttcctgtac gcgtcagccc gcaccgttac ctgtggtaat ggtgatggtg gtggtaatgg
     6121 tggtgctaat gcgtttcatg gatgttgtgt actctgtaat ttttatctgt ctgtgcgcta
     6181 tgcctatatt ggttaaagta tttagtgacc taagtcaata aaattttaat ttactcacgg
     6241 caggtaacca gttcagaaac tgctatcaga cactcttttt ttaatccaca cagagacata
     6301 ttgcccgttg cagtcagaat gaaaagctga aaaatactta ctaaggcgtt ttttatttgg
     6361 tgatattttt ttcaatatca tgcagcaaac ggtgcaacat tgccgtgtct cgttgctcta
     6421 aaagccccag gcgttgttgt aaccagtcga ccagttttat gtcatctgcc actgccagag
     6481 tcgtcagcaa tgccatgact cgttcgcgta aagcttgcag ttgatgttgg tctgccgttg
     6541 tgtcactttt cgtcggttgt tgtattaatg ttgctaattg atagcaatag accatcaccg
     6601 cctgccccag attgagcgaa ggataatccg ccaccatcgg cacaccagta agaacgtcag
     6661 ccaacgctaa ctcttcgtta gtcagcccgg aatcttcgcg accaaacacc agcgcggcat
     6721 ggctcatcca tgaagatttt tcctctaaca gcagcaccag ttctactggc gtggcgtagt
     6781 aatgatattt cgcccgactg cgcgcagtgg tggcgacagt gaaatcgaca tcgtgtaacg
     6841 attcaaccag tgtcgggaaa actttaatat tatcaataat atcaccagat ccatgtgcga
     6901 cccagcgagt ggctggctcc agatgtgcct gactatcgac aatccgcagt tcgctaaaac
     6961 ccatcgtttt cattgcccgc gccgctgccc caatattttc tgctctggcg ggtgcgacca
     7021 gaataatcgt tatacgcata ttgccactct tcttgaccaa atgaccgcga acagggtgat
     7081 cactgtcaac ttattacacg gtgcgaattt acaaatcctt aacgtaagtc gcagaaaaag
     7141 ccctttactt atcttaaaaa aggctaaact atttcctgac tgtactaacg gttgaattgt
     7201 taaaaaatgc tacatatcct tctgtttact taggataatt ttataaaaaa taaatctcga
     7261 caattggatt caccacgttt attagttgta tgatgcaact agttggatta ttaaaataat
     7321 gtgacgaaag ctagcattta gatacgatga tttcatcaaa ctgttaacgt gctacaattg
     7381 aacttgatat atgtcaacga agcgtagttt tattgggtgt ccggcccctc ttagcctgtt
     7441 atgttgctgt taaaatggtt aggatgacag ccgtttttga cactgtcggg tcctgaggga
     7501 aagtacccac gaccaagcta atgatgttgt tgacgttgat ggaaagtgca tcaagaacgc
     7561 aattacgtac tttagtcatg ttacgccgat catgttaatt tgcagcatgc atcaggcagg
     7621 tcagggactt ttgtacttcc tgtttcgatt tagttggcaa tttaggtagc aaacatgcag
     7681 accccgcaca ttcttatcgt tgaagacgag ttggtaacac gcaacacgtt gaaaagtatt
     7741 ttcgaagcgg aaggctatga tgttttcgaa gcgacagatg gcgcggaaat gcatcagatc
     7801 ctctctgaat atgacatcaa cctggtgatc atggatatca atctgccggg taagaacggt
     7861 cttctgttag cgcgtgaact gcgcgagcag gcgaatgttg cgttgatgtt cctgactggc
     7921 cgtgacaacg aagtcgataa aattctcggc ctcgaaatcg gtgcagatga ctacatcacc
     7981 aaaccgttca acccgcgtga actgacgatt cgtgcacgca acctgctgtc ccgtaccatg
     8041 aatctgggta ctgtcagcga agaacgtcgt agcgttgaaa gctacaagtt caatggttgg
     8101 gaactggaca tcaacagccg ttcgttgatc ggccctgatg gcgagcagta caagctgccg
     8161 cgcagcgagt tccgcgccat gcttcacttc tgtgaaaacc caggcaaaat tcagtctcgt
     8221 gctgaactgc tgaagaaaat gaccggccgt gagctgaaac cacacgaccg tactgtagac
     8281 gtgacgatcc gccgtattcg taaacatttc gaatctacgc cggatacgcc ggaaatcatc
     8341 gccaccatcc acggtgaagg ttatcgcttc tgtggtgatc tggaagatta atcggcttta
     8401 ccaccgtcaa aaaaaacggc gctttttagc gccgttttta tttttcaacc ttatttccag
     8461 atacgtaatt gatcgtccat tgtaacttct ttactggctt tcattttcgg cagtgaaaac
     8521 gcataccagt cgatattacg agtcacaaac atcatgccgg ccagcgccac caccagcaca
     8581 ctggttccca acaacagcgc gctatcggca gagttgagca gtccccacat cacaccatcc
     8641 agcaacaaca gcgcgagggt aaacaacatg ctgttgcgcc agcctttcaa taccgcctgc
     8701 aaataaatac cgttcattaa cgccccaatc agactggcga ttatccatgc cacggtaaaa
     8761 ccgatatgtt cagaaagcgc cagcaagagc aaataaaaca tcaccaatga aagccccacc
     8821 agcaaatatt gcattgggtg taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc
     8881 ataaaagtca gtgcaatcag cagaatggcg tacttagtcg cccagtccgt taattggtat
     8941 tgatcggctg gcgtcgtcac tgcgacgcta aacgcgggga agttttccca gccggtatca
     9001 ttgcctgaag caaaacgctc accgagatta ttagcaaacc agctgctttg ccattgcgcc
     9061 tgaaaacctg actcgctaac ttcccgtttg gctggtagaa aatcacctaa aaaactggga
     9121 tgcggccagt tgctggttaa gggcatttcg ctattacgcc cggcaggcac cacagaaaga
     9181 tcgccggtac cgcttaaatt cagtgccata ttcagctgca ggttctgctt ccgccagtcc
     9241 ccttcgggta aagggatatg cacgccctgc ccgccttgct ctaacccggt accgggttca
     9301 atggtcagtg ccgttccgtt aacttcaggc gctttcacca caccaatacc acgcgcatcc
     9361 ccgacgctaa tcacaataaa gggcttgcct aagatgatat ttggcgcgtt gagctcgcta
     9421 agacgcgaaa catcgaaatc ggcttttagc gttaaatcac tgtgccagac ctgaccggta
     9481 taaatcccta tcttgcgttc ttccacgttc tgattgccat caaccatcaa tgactcaggt
     9541 aaccaaaaat ggataaaact tcgtttccgc tccacggttt tatcctcttc ctgcaccgta
     9601 taaagctcgg tcacaggaat agcgataagc ggcccaacga gtttttgcgg cccgctggta
     9661 ctttggcgaa tcgcatcttc cacatcgcta cggtaatcgg cacgttcgac aatcacttgc
     9721 cgaatcagca ttatcggaat caacaacagc aatactgcac caaacaaggt ggtcattttc
     9781 cagaacaggg gggatttcaa catagtcttc tcctttgcaa tggcagcagg atacgaaggc
     9841 tatgtgggaa gaatttgaag ctatgtgaag tgacggtgaa gtcgaagcga ggccagcacg
     9901 ccaccttcct gcacgttgcg cagcgtgact tcgccgttaa acaaacgggc gacctcactg
     9961 acgaacgcca accccagacc gctgcttttt tgcccatttg cacgcggcaa agagtaaaag
    10021 cgttcaaaaa tacgtgacag cgcgtagtca ggaataccac taccggtatc cagcacctta
    10081 agggcgacgt gttcctgatc cacttcggcg cttagcgtta tgcgaccgct ctcgggggta
    10141 aaatcgatgg cgttatccag taaattcccc agcgcctgct ccagtaacgc cggttcagca
    10201 gcaacattaa cctcagtggg cataacatgt agagtgatgt ttttttctgc caactgcacg
    10261 gtgcgcgctt cgctgacgcg gcgaaataat gccgccacat caacaacagt cagaacgact
    10321 tcctgacgat tctccagtct tgcctggcgt agtaacgttt ctaccaatgc ctgcatacgc
    10381 gcattttgcg tcagaatgtt gtcggtaaaa cgagccacca cttccggtgg cggaccttcg
    10441 cgtaaaattt ccgccgcgcc acgaatcgcc gccagtgggc tttttagctc atgggttaac
    10501 gcataaacat actgctcaat atagtttttc ccttccagct tcacgcgcat actttccagc
    10561 gcctgcgcaa gcttacgcaa ctcgctactg ccgagttcag gaagaggaac gggcttattg
    10621 tcagtgacgg aatcggcata gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc
    10681 atgcctgcgc caatcaccag tgcaatcccc agcaaaatgg cgctggccca taaaattcgc
    10741 cgctcgctac gcttaatgac cggagccatc gccgcgttcg gtttgcctac gctcaaaacg
    10801 ccaataagcc gcgagccgtc cataatcggc gcggcaacat acatcaccga actttcggga
    10861 tcggcaggat tttgcaacgt gctgcgcgca ccatactgac cacgcaacgt tagccagacg
    10921 tcattccagc gcgaataatc ctgcccaacg gctttatttg ccgaatcgaa caatacttta
    10981 ccctgcgaat cggtcatata gacatgatat tcattgcgca ctttgttaat gccaccgata
    11041 ttggcgcgaa acggacgatg ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt
    11101 gggtccccag agagcaaatc gggacgcgcc agctccgcca gcaacgttgc ggtgtcgatc
    11161 aacgtcccct ccgttgctct tcgcacgccc ggtttaactt ctttgacaaa aatagccagt
    11221 acgaaccagg ccgccaccgc caccagtaaa aaatagccca gcagcaaccg catgccgata
    11281 cgcattacag gcccctcagg ctatatccca tgccgcgatg agtattaatc ggtgaaagat
    11341 cggggttgat ggcacgcagc ttggcatgca acgttttaat gtgggtatca acggtgcgat
    11401 cgtaggtgtc ctgcgcatct tcccacacgc tatccatcag ttgctggcgg gaccagacgc
    11461 ggcctggcga cttgagtaac gtcttcagca ataaaaactc ataccgcgtc agcgttaatg
    11521 gcgtgtcaaa ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga
    11581 tgacgggaga cggcgacgag aacttcttca cccgacgcag taaggtgcgc accctggcgc
    11641 acacttcgcg gggtgaaaac ggtttagcca catagtcgtc agcgccaatt tccagcccaa
    11701 gcaggcgatc gacctcttca cttcgggccg tcaggaacag tacaggtaac gccggatgga
    11761 gcgccagtaa ctggctgcac aattcaaagc cgctgatatc aggcaaacca acgtcgagaa
    11821 tcatgacgtc gggaaccagc tggcgagctt tatccagcac cggcaagcct cgctcaaaga
    11881 cctcgacggc aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct atcccttgct
    11941 catcttccac taaccagacc gtttcccgtt gtataaatcg cctctgttat tgccgccacg
    12001 gcatgacagg aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt
    12061 aagccagata agcgagcgcg ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact
    12121 taaagaccag ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg ccgtttttaa
    12181 tacgatccga cagttcaatc ggcccgactt gctgacaaga aatagccgca tcggaggtat
    12241 cttccgccag acccaatccc cctttaatac cacctgtttt cgcccggctc acataacagg
    12301 tgacattttt cacatcggga tcgtcaaagg cttccacaac aattttgtga tccgggccga
    12361 tcattttaaa tacggtgtcg accgaaccaa tctcttctgc atgagccaat ggccccagca
    12421 ttatcaggct taaagacagg atcaaatgct tgtatttcat attgttacca tttttatcta
    12481 ttacacgagc aattagttcg tcacggtaaa taacatattg attaagaaca aaatctcaat
    12541 tcttttattt ccgtcaagcc ctacaacata ctctactaag aaaaaaacac tgaatgctaa
    12601 aacagcaaaa aatgctatta tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg
    12661 aggatatttt atggatcagg ccggcattat tcgcgacctt ttaatctggc tggaaggtca
    12721 tctggatcag cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca
    12781 cttacagaga atgtttaaag atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg
    12841 tcgtttgtcg aaatcggcgg tcgcactacg cctgactgcg cgtccgattc tggacatcgc
    12901 gctgcaatac cgcttcgact ctcaacagac atttacccgc gcattcaaga agcagtttgc
    12961 ccagactcct gcactttacc gccgttctcc tgaatggagc gcctttggta ttcgcccgcc
    13021 gctacgtctg ggtgaattca caatgccaga gcacaaattt gtcaccctgg aagatacgcc
    13081 gctgattggt gttacccaga gctactcctg ttcgctggag caaatctctg atttccgcca
    13141 tgaaatgcgt tatcagttct ggcacgattt tctcggcaac gcgccgacca ttccgccagt
    13201 gctctacggc ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac aagaggtatt
    13261 ctataccacc gcgttagccc aggatcaggc agatggctat gtactgacgg ggcatccggt
    13321 gatgctgcag ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca
    13381 ggagtttatc ctgacggtat acggaacgtg catgccaatg ctcaacctga cgcgccgtaa
    13441 aggtcaggat attgagcgat actacccggc agaagacgcc aaagcgggag atcgcccaat
    13501 taatctgcgc tgtgaactgc tgattcctat ccgccgttaa cgctgcagct catctaatgc
    13561 aggggcgtcc agatgcgaga tgtcccctgc ggtttcaacg acccagccag acgccagcca
    13621 caggctttcc tgataatcca cgcgcgaaat agaacagtta cgcagacgta agcggcgcgc
    13681 tgcccatgct ggtaatccga gaatcgtact caccagacat cccagtgcaa taccatggct
    13741 caccagcagc ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg ccgcattgac
    13801 gcgatcgctg agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt
    13861 gaccagctgc cgacgccagt tctcttcttc ttcggtcaga gaatcgatat gtcttttttc
    13921 cagcacaccc atgtttaatt cacgcaggcg agaatcaaag atgatgtcac agccgcaggc
    13981 ctgggcgatg atttccgccg tacgccgggt gcgtcctaaa tcgctactga taatatgcgt
    14041 aatgccaagc tctttggcac gggttgccac ctgcatcgcc tgttgctcac ctttggcggt
    14101 cagcgggctg tcagactggc cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg
    14161 gcggactagg tatacctgta acatgctttt tttccgtata ctgctgtaac gttaattttc
    14221 tgagtaatgc tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag
    14281 gccattctgc aggcatttca cgagatcttc ggcgaaggat cctgccatat tgcatccgtt
    14341 gccgtcgaga gcggtgtacc ggaacagccc tttggcagtg aggaaacgcg cgctggcgca
    14401 cgaaatcggg tagcaaatgc ccgccgttta cttccagagg ctgatttttg ggtggcgatt
    14461 gaagctggca tcgatggtga cagcactttc agttgggtgg tgattgaaaa caccagccag
    14521 cgcggcgagg cgcgttcggc tactttgccg ttacctgcgg tgattctgca gaaagtgcgt
    14581 gaaggtgagg cgcttggtcc cgtgatgtcg cgttataccg gtattgatga gattggtcgt
    14641 aaggaagggg cgatcggcgt gtttaccgcc gggaaactca ctcgcgccag cgtctatcac
    14701 caggcggtga tcctggcgct gagtccgttt cacaatgccg tgtatcaggc attgtaggac
    14761 ggataaggcg ttcacgccgc atccggcatt cggtgcacga tgcctgatgc gacgctgccg
    14821 cgtcttatca ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactggcg
    14881 cagctccacg ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcccgc
    14941 gccgagttca ttttttaact cacgctgact catttcgcca cgcaacagct cttcgacaat
    15001 acgcacgcga gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg
    15061 taaatggaga tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg
    15121 gtgacgctgt tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca
    15181 ttgttagcgg gggaagcaaa atgcctcccc cggttgtact cgctaaagag tacgatagca
    15241 tatcataaac gtgcggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt
    15301 ttatccccca tgaaataacg gtagtaagcg tcataagcca gcacgttctt cacataaccg
    15361 cgcgtctcgg agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg
    15421 ttgccaagcc aggttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata
    15481 cggttattgc caaactgctg ataaacatat tgcaggtaac tggtgccaat gttgatattc
    15541 gtttccggat ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc
    15601 gtatgggtcg ctgtaccagg cataatctgc atcaggccgc tggcccctac cggtgatttc
    15661 actttcggat tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc
    15721 tctttcccac tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc
    15781 agatgatccc aaagcttccc ggcgatcgtt gcctgaacgc taagatccca ccattggtta
    15841 ttgaaagcat accgagccag ttgagcctgc tctgtttttg acttgctctt caccagattg
    15901 gcccactcgc tacgcgcggt gttatcgaga ttccagtaca tcaactcgcg cacgcgcgcc
    15961 atctccggcc cctgagtcag ggcgctgtca acattctgcg gcgctttatc aatcttcagc
    16021 tcatactctt ctccgatgcg ttgtgctgca accatcgggt agaaaccacg ctgttgcatg
    16081 agttgatgca aaatctcttt cgcttcagct tcacgtccgc gttccagcaa taaatccgcc
    16141 tgccagtaac gccattcatc tttctctttt gcttccatcg gcagacgcgc cagccaggta
    16201 ttcaggccgc gacgatcgcc ggtgccaagt gccattcgca cacggcgttc aatcaacgac
    16261 gtggattgcg agcgcataat ggcgtcatcg cgccatttcg cctgctcgtc ggtgacatcg
    16321 ttgcccatca aacgccaggc gacgatatcg cgcagctcct gaatctgatc ttcattaagc
    16381 tgctgcgcct gggcaagcga cgggatcatc agccgtgcat tttcagcatc ctgccgcgcc
    16441 acactggcaa acgccaccgc tgccatttga cgggtaaaat cggtcgctcc ggttgtacgc
    16501 gcgaaggtca gtaccgtatt agggttgttc gccagtgaaa tgattgccga ggcgatagtc
    16561 tggtaatcgg caggcatctg ccctgccagc actgttacca ggccagtgtt acccgctttc
    16621 atcgccagac ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag
    16681 acgctaaata acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc
    16741 gccccttgcc aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat
    16801 tgcgcttcgg tagttccggg cttttccggg ctaaaggcta acaagccacg ccagtcttca
    16861 cgccgcgcca gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg
    16921 ttagcgcgaa caaagttagt gaccgtcacc gccggttgat tcatcagatc atcggtgatc
    16981 tggcggtatt ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg
    17041 accacatcca tttgtcgatt atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc
    17101 tcatccagtg agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc
    17161 aacagccgcc aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac
    17221 atcaatgcag cgtcatgccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc
    17281 acgttacgga cactttttta cttttactgc gaaggtgatc gaccaactgg gattaggcgg
    17341 ggaaaaaggc tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt
    17401 ccaacgtggc tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac
    17461 gtcatatttt gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg
    17521 gtctgaacgg cgcgggtaag tctaccctgc tgcgcattat ggcaggcatt gataaagaca
    17581 tcgaaggtga agcgcgtccg cagccagaca tcaagattgg ttacctgccg caggaaccgc
    17641 agctgaaccc ggaacacacc gtgcgtgagt ccattgaaga agcggtttct gaagtggtta
    17701 acgccctgaa acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg
    17761 acaagctggc cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata
    17821 acctgaacgt acagctggag cgtgcggcgg atgcgctacg tctgccggac tgggacgcga
    17881 aaatcgctaa cctctccggt ggtgagcgtc gtcgcgtagc gttgtgccgc ctgctgctgg
    17941 aaaaaccaga catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg
    18001 cctggctgga acgcttcctg cacgacttcg agggcaccgt ggtggcgatt acccacgacc
    18061 gttacttcct cgataacgtt gcaggctgga tcctcgaact tgaccgcggt gaaggtattc
    18121 cgtgggaagg caactactcc tcctggctgg agcagaaaga tcagcgcctg gcgcaggaag
    18181 cttcacaaga agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg
    18241 gaactaaagg ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca
    18301 gcaccgaata tcagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc
    18361 tgggcgataa agtgctggaa gtcagcaacc tgcgtaaatc ctacggtgat cgcctgctga
    18421 ttgatgacct gagcttctcg atcccgaaag gggcaatcgt cgggatcatc ggtccgaacg
    18481 gcgcgggtaa atcgaccctg ttccgtatga tctctggtca ggaacagccg gacagcggca
    18541 ccatcacttt aggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg
    18601 ataacagcaa aaccgtttgg gaagaagttt ccggcgggct ggatattatg aagatcggca
    18661 acaccgagat gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg
    18721 gtaaacgcgt tggtgaactt tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc
    18781 tgcaggttgg cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa
    18841 ccctgcgcgc gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc
    18901 acgaccgttg gttcctcgac cgtatcgcca cgcacatcct ggactaccag gatgaaggta
    18961 aagttgagtt cttcgaaggt aactttactg agtacgaaga gtacaagaaa cgcacgctgg
    19021 gcgcagacgc actggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa
    19081 aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa ccaagaaatt tcaatacgtt
    19141 gtgaatctcc aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg
    19201 ccggatacgg cgtgaacgcc ttttccggcc tacaatagcc ttagtatccg caatttcgta
    19261 gcgggtcacc cgtgctgatt cttttaaacc ttgtttaatc tcgacagcct cttccagaga
    19321 ggttttcaac tcatcaaaaa acttatttta tacctcgcct ttgagccgtt tcatcgcggt
    19381 tatctctgct cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtca
    19441 taatcgtcct cttcaacccg cacgaattcg atattgttct cttccagcat ctccaccagc
    19501 aagttctgga actctttgcg atccaccgaa ctgccgaggc tgcgtaaacc atccgccacc
    19561 cacggcgtgt tgttctccag caggatcacc agatcgaaac ggtattcatc aatcaacgcc
    19621 tgtacgaacg gatgctcacg cccttcgtac tttttgcaga acgcctgggt agtgacaaaa
    19681 tcagtgtcaa taaacgccac tttattggca tatttcactg caaaatcaat gtattgtgcg
    19741 tggcccagcg cgattttatc gtaatcggaa tactgcaatg cgatctcatc accgccgagg
    19801 tgtgaaaaga cataatcgcg accatattcc cacgcactgg tggtattgaa gatattggca
    19861 agtttgttta ccagggtgga tttaccgctc gactcgccac caaggatcgc cacggtacgt
    19921 acaaagaacg gcttcacttc cgtaggaata tattcccagt agcggaacgg gttttcgcgg
    19981 atctgcgcac cgctgatact cataaaggta cgtttcggat cgaccagcac cgtatcgatc
    20041 cccagatgtt ccatatactg tggcgcatcg gcttcttccg aggtgtagat caggtccggc
    20101 tgaatcccct tttcagccat aaactttttg atgccgttgc tccacacatc ccagccgtgc
    20161 ggatacggct ccatgccctc ctcgttgaaa gcatgaatgc gaatattttt ctgatattta
    20221 aaagtttgta ataaccagcg cagacgatcc ggcaccgtgg gctgctgcga catggcgctg
    20281 tcttcaaaca acgcgcggtc gcgggtatcg tcaaaaccca taatgatatg cagctcgtca
    20341 acctggctac aggcgcgctg gataaggtag atatgtccgg tatgcagcgg gtagaactta
    20401 ccgaatacga caccaatggt tttcttctgt cggggaaatt caagccccaa aaaacggtgc
    20461 aacgcctcca gcttttgcgc gctggggctt ttaattttgg cattcagtaa ctggcttaaa
    20521 taccctttgg tcataccgct ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg
    20581 atggcagttt tcaggtaatc aaatgacgac atatctccct ccgtatctct cattataagt
    20641 cgtcgaacac gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg
    20701 gcgctttttt cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg
    20761 cttctgaaat tcgttcctga ccgctgggca ccgggcggat ctcccctgcc agcccgactt
    20821 caccaaacac caccagatcc tgtggcagcg gtctgtcacg caggctggaa accatcgcca
    20881 gcagtaacgc taaatcggca ctggtttcgg ttaccttcac gccgccgacc acgttcacaa
    20941 atacatcctg atcggccatt tgcagaccac cgtgacggtg caagacagcc agcaggattg
    21001 ccagacggtt ttgttccagc cccactgcca cgcggcgcgg attcgccatc atcgagtgat
    21061 cgaccagcgc ctgaatctcc accagcaacg gacgcgttcc ttcccacacc accatcacgg
    21121 agctgccgga ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt
    21181 cacgcagccc ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt
    21241 ttttatggct gcgcaaggtg cgaaaacggg agtcggcatc accatccaga agcaccgaac
    21301 agtcgataca gtgttccagc actttagggc cagccagcga gccatctttg gttacgtgcc
    21361 caaccatgac aatcgccaca ccgcgcgttt tggcgaagcg cgtcagataa gccgccgttt
    21421 cacgcacctg cgccacgcta ccaggcgatg attgtacatc cgccatatgc atcacctgga
    21481 tcgagtcaat taccatcagt ttcggttgct cttcttcggc aatcaggcag atctgttcga
    21541 tgctggtttc cgacaacata ttgagattgt cagtcggcag gccaaggcga tgagcgcgca
    21601 ttgccacctg ttgcagcgac tcttcgccag tgacatacag cgttttcatc tgctgggcca
    21661 gtttgcacag cgtttgcagt agcagcgtgg atttccccgc acctgggtta ccgccaatca
    21721 gaatggcgct tcccggcacc acgccgccgc ctagtacgcg gtcgaactct ttaaatccgg
    21781 tggaaaaacg cggcagctct tcaaggctga tatcggagag tttctggact tttgccaccc
    21841 cggcgctacc ggcatagccg ctgagacgct cgttacgcgc caccgttggc gacgcagcaa
    21901 gacgcacctc ggtgatggtg ttccaggcat gacaggcact gcactgcccc tgccagcgcg
    21961 gataatcggc cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt
    22021 acctcgttaa tgctgtgccg cccgcaggat ggcgggcgag caattacttc tgattcaggc
    22081 tgccagagag gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgcct
    22141 tttcattcac ttttggcttg gcatggtagg caatccccag ccctgctgct ttgatcatcg
    22201 gcaggtcatt agctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct
    22261 gcgcgaggcg agtcaaagtt ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat
    22321 tgccggtaaa tttaccgtcc atgatctcca gttcattggc taccacggcg gtcaggcgca
    22381 gcttgtcgcg cagatattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc
    22441 agcccagcgt ttccagcttg agcaccagtt gcgttaagcc tggcatcagc ggcagatttt
    22501 cacgcacctg ttgcagaata ttggcgtcag cgcctttcag cgtcgccaca cggctgcgca
    22561 ggctggcggt aaaatcgagt tcgccgcgca tcgcccgttc ggttacttcc gccaccatct
    22621 cgcccgttcc ggccagtttg gcaatttcat caatacattc aatctggatg gcggtggaat
    22681 ccatgtccat caccagcaaa cccggcgtgc gcaggtgcgg gattttcccc agcggggcga
    22741 catccagttg cgcttcatgg gccaggcgcg tagcccgtgc ggtgagtgaa cctgccagac
    22801 gaatcacctg ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcacccagtt
    22861 tgctctggta ttgggtcaga cgctgtttat ccagcccacg accatacagc agccagccgc
    22921 tacgacctgc gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac
    22981 caggccataa agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggatc
    23041 ctgtaaaatc gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc
    23101 tggcaacatt aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt
    23161 ccggctgcat cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cgctgatgca
    23221 gggagcgtca tggtttagtc aaaaccacca gcgacaacgt aatccacagc tggaagaact
    23281 ggcccgcacc ctggcgcgtc aggtaacgct gaacgttgca ccgctgatgc gtaccgactc
    23341 accggatgaa aaacgcattc aggcgatcct cgatcagtta acggatgaaa gccgtatcct
    23401 cgacgcgggt gtgtatgacg aacaaggcga tcttatcgca cgttctggcg aaagcgtcga
    23461 agtgcgcgac cggctggcgc tcgacggtaa aaaagcgggc ggctatttta accagcagat
    23521 tgtcgaacca attgccggga aaaacggacc gctcggctat ctgcgcctga cgctcgacac
    23581 ccatacgctc gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat
    23641 gttgctgctt tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg
    23701 tacccgctgg cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc cggaagagga
    23761 agaaagcgag aaaaaagagt gacccattac tacaagaaag gaaatcgtta tgtccacatt
    23821 acgcctgctc atctctgact cttacgaccc gtggtttaac ctggcggtgg aggagtgtat
    23881 ttttcgccag atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt
    23941 agtgattggt cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga
    24001 taacgtccgc ctggcacggc gcagtagcgg tggcggcgcg gtgttccacg atctcggcaa
    24061 tacctgcttt acctttatgg ctggcaagcc ggagtacgat aaaaccatct ccacgtcgat
    24121 tgtgctcaat gcgctgaacg cgcttggcgt cagcgccgaa gcgtccgggc gtaacgatct
    24181 ggtggtgaaa accgccgaag gcgaccgcaa agtctcagga tcggcctatc gcgaaaccaa
    24241 agatcgtggc ttccaccacg gcaccttgct gctcaatgcc gaccttagcc gcctggcaaa
    24301 ctatctcaat ccggataaaa agaaactggc ggcgaaaggc attacctcag tgcgttcccg
    24361 cgtgaccaac ctcaccgagc tgctgccggg gatcacccat gagcaggttt gcgaggccat
    24421 aaccaaggcc tttttcgccc attatggcga gcgtgtagaa gcggaaatca tctccccgga
    24481 caaaacgcca gacttgccaa acttcgccga aatctttgcc cgtcagagta gctgggaatg
    24541 gaacttcggt caggctccgg cattctcgca tctgctggat gaacgcttta gctggggcgg
    24601 cgtggaactg catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tgtttaccga
    24661 cagcctcaac cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gcctgtaccg
    24721 cgcggatatg ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg accaggaaaa
    24781 agagctacgg gagttgtcga cgtggatagc gggggcggta aggtaactcg ctgattaaaa
    24841 taccaaagga agggaatatg atcgatctgg aaaaccagga gcgggagatt atcaatttaa
    24901 tgttaagcca gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg
    24961 ctgaagtaag taaaatgtta ggtatctcga ttaattctct aaaatagatc gaaaagacgg
    25021 aacggtttag ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt
    25081 tgctgatatg cccttcctgg atgacggctg aacataaatg aatagatt
//
LOCUS       DAJVOY010000043        25115 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVOY010000043 DAJVOY010000000
VERSION     DAJVOY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.043"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..500)
                     /locus_tag="OQL90_001577"
     CDS             complement(<1..500)
                     /locus_tag="OQL90_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1368398.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQG"
     gene            complement(675..1100)
                     /locus_tag="OQL90_001578"
     CDS             complement(675..1100)
                     /locus_tag="OQL90_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1368399.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(1116..1886)
                     /locus_tag="OQL90_001579"
     CDS             complement(1116..1886)
                     /locus_tag="OQL90_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCS1368400.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(1908..2650)
                     /locus_tag="OQL90_001580"
                     /pseudo
     CDS             complement(1908..2650)
                     /locus_tag="OQL90_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2657..3619)
                     /locus_tag="OQL90_001581"
     CDS             complement(2657..3619)
                     /locus_tag="OQL90_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1368401.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(3642..4067)
                     /locus_tag="OQL90_001582"
     CDS             complement(3642..4067)
                     /locus_tag="OQL90_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1368402.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDPPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(4051..4374)
                     /locus_tag="OQL90_001583"
     CDS             complement(4051..4374)
                     /locus_tag="OQL90_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1368403.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            4499..4975
                     /locus_tag="OQL90_001584"
     CDS             4499..4975
                     /locus_tag="OQL90_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368404.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKKAIVAFILSGNATEPSWVDYDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            5294..5446
                     /locus_tag="OQL90_001585"
     CDS             5294..5446
                     /locus_tag="OQL90_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1368405.1"
                     /translation="MDTIELGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            5458..5778
                     /locus_tag="OQL90_001586"
     CDS             5458..5778
                     /locus_tag="OQL90_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368406.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKLLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENYFATKSINS
                     FDSK"
     gene            complement(5756..6031)
                     /locus_tag="OQL90_001587"
     CDS             complement(5756..6031)
                     /locus_tag="OQL90_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368407.1"
                     /translation="MSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVM
                     DKNEVVRQSLVDYIDSLQPGTFNHESFNHEKEHVKSVINSLILNQKN"
     gene            6593..6781
                     /locus_tag="OQL90_001588"
     CDS             6593..6781
                     /locus_tag="OQL90_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCS1368408.1"
                     /translation="METLLPNINTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            6778..6969
                     /locus_tag="OQL90_001589"
     CDS             6778..6969
                     /locus_tag="OQL90_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCS1368409.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            7062..9533
                     /locus_tag="OQL90_001590"
     CDS             7062..9533
                     /locus_tag="OQL90_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCS1368410.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQHSENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQSGGGNCPDCG
                     AVMGDATYQETFDEENQVEVQENDPKEMEGAEHPHKENAGSAQDHASDSETGETADPL
                     IAMNGHHVITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYISVIWQKLIPNQADF"
     gene            9598..9846
                     /locus_tag="OQL90_001591"
     CDS             9598..9846
                     /locus_tag="OQL90_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCS1368411.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            9824..10954
                     /locus_tag="OQL90_001592"
     CDS             9824..10954
                     /locus_tag="OQL90_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1368412.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRRLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(11000..11638)
                     /gene="ompW"
                     /locus_tag="OQL90_001593"
     CDS             complement(11000..11638)
                     /gene="ompW"
                     /locus_tag="OQL90_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1368413.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            11878..11967
                     /locus_tag="OQL90_001594"
                     /pseudo
     CDS             11878..11967
                     /locus_tag="OQL90_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            11995..12738
                     /locus_tag="OQL90_001595"
     CDS             11995..12738
                     /locus_tag="OQL90_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1368414.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDSVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            12768..13307
                     /locus_tag="OQL90_001596"
     CDS             12768..13307
                     /locus_tag="OQL90_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1368415.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            13412..13810
                     /gene="yciA"
                     /locus_tag="OQL90_001597"
     CDS             13412..13810
                     /gene="yciA"
                     /locus_tag="OQL90_001597"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1368416.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(13850..14578)
                     /gene="tonB"
                     /locus_tag="OQL90_001598"
     CDS             complement(13850..14578)
                     /gene="tonB"
                     /locus_tag="OQL90_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1368417.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            14793..15089
                     /locus_tag="OQL90_001599"
     CDS             14793..15089
                     /locus_tag="OQL90_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1368418.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            15389..16642
                     /gene="kch"
                     /locus_tag="OQL90_001600"
     CDS             15389..16642
                     /gene="kch"
                     /locus_tag="OQL90_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1368419.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(16697..16870)
                     /locus_tag="OQL90_001601"
     CDS             complement(16697..16870)
                     /locus_tag="OQL90_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1368420.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            17013..18473
                     /gene="cls"
                     /locus_tag="OQL90_001602"
     CDS             17013..18473
                     /gene="cls"
                     /locus_tag="OQL90_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1368421.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            18508..18837
                     /locus_tag="OQL90_001603"
     CDS             18508..18837
                     /locus_tag="OQL90_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1368422.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(18890..19894)
                     /gene="oppF"
                     /locus_tag="OQL90_001604"
     CDS             complement(18890..19894)
                     /gene="oppF"
                     /locus_tag="OQL90_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1368423.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(19891..20904)
                     /gene="oppD"
                     /locus_tag="OQL90_001605"
     CDS             complement(19891..20904)
                     /gene="oppD"
                     /locus_tag="OQL90_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1368424.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTTVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            complement(20916..21824)
                     /gene="oppC"
                     /locus_tag="OQL90_001606"
     CDS             complement(20916..21824)
                     /gene="oppC"
                     /locus_tag="OQL90_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1368425.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(21839..22759)
                     /gene="oppB"
                     /locus_tag="OQL90_001607"
     CDS             complement(21839..22759)
                     /gene="oppB"
                     /locus_tag="OQL90_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1368426.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(22845..24476)
                     /gene="oppA"
                     /locus_tag="OQL90_001608"
     CDS             complement(22845..24476)
                     /gene="oppA"
                     /locus_tag="OQL90_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1368427.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            24767..25063
                     /locus_tag="OQL90_001609"
     CDS             24767..25063
                     /locus_tag="OQL90_001609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368428.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
BASE COUNT         6557 a         6211 c         5843 g         6504 t
ORIGIN      
        1 ccctgcttgc gccagttata atcccagtac accatacccg gataatgcgc aacagctcgc
       61 cgcccacact cgttaacctg ctgcatattt ttccagcgtg aaatgcccag tgaactgctg
      121 atcacactga aaccattttc agcagcgtac aacgctgtcc gctcaaaacg catgtcaaaa
      181 cacatggtac aacggatccc cctctcaggc tcccattcca ttcctttggc acgttcaaac
      241 cagttgtcgg tgtcgtaatc agcatcgata aacggcacgc cgtgttgttc agcaaagcga
      301 atattttcat ccttacgaat taaatactct ttctgaggat gaatgttcgg gttgtagaaa
      361 aagatggtgt agtcgattcc cgaggcctga agcgcctcca tcacttcacc ggaacatgga
      421 gcacagcaag agtgcagtag tagtttgttt gccccgtttg ggagctccaa tttaggccgt
      481 ttgaaatcag caatagtcat aaatattttt attggggtca tgaaaatagc acagagtgta
      541 gcatcagagc agggctatcg ggaatatatg tctaaatctg gtaatatctg gttttgacgc
      601 aaagcggaca accacgctgg ctctaccctg cgccatgaaa atgtcaattc acatctgaac
      661 taatgctctt taatctagta acgtctaaaa tacctaacat ttccttgata aaatgccagt
      721 acacgctgca tagcttcgct cttccggcac tcgcgacaga ttatattcag gcgcctgtcg
      781 tagcggcgta tttcgccgtc tggtaacgac cagataaggt ccggatcaac cactgcaggt
      841 ttcttcacct ttgcccttga gagttttttg cgagcatttt gccagtcctt acgcgcctgt
      901 tcagacggga ataacccgta accagagttg tatacatcgc cactggcaac cagctctctg
      961 gcgagaacac tcatcagata tcttgtcgca cctgtcttgg cttccagttg ccgcaacgtc
     1021 tcgcgaccgc tcagacgtac aagttcaaca acctgccctt taattttttc ccgctcttct
     1081 tgtgtaaata cttttgccat aagcgcctcc ggcaatcact tttccgatac aacacggcgg
     1141 gaagaatcag taatctgtcg aacaatatcc cggtgcttgt tcagctcccg cagcgcggcg
     1201 cagactcgct cccacttctg aacatcactt ttcgccctgc gcagcgccag gtttgccctg
     1261 cgaagggacg gaaaaatcag ctcatctgct tgcgtttcgg taaacgatgg caacggctgc
     1321 acaatgtccg ccacagtttc tgttttaatt tcttcctgtg ttgcggcttc ccggactggt
     1381 aacgcagcac ctgctggctg aggaaaggcc ttaccatcac tttccgttac cagcgcggct
     1441 ttcggctctg ctggtaaatt atcgcccggc atgcagtaac gaaatttacc gttctgatta
     1501 acgcgtgcca gccgccccgt tgcggttacc accgccagcg tggaagcaac cttgcgagta
     1561 ctgacaccga acttacccgc cagttcctca cacgttttag ccccatcctg accgataaac
     1621 tcaatcatca tgtctgcggt aactttttgt tcgacctccc cggtcagcat atcctgtgct
     1681 tcagatttta ctggccgctc ttcggttacc cgggattcac cttcgccagc cagaaaccag
     1741 gtgtgaccag ttttatcaac gacgcctttt cttttgagtt cccacagctc gttgacagcc
     1801 tcttcacgac tgattccaag gcgagctgcc accacatgtg aagaggcttt tttcagtgct
     1861 ttcagtgcgt cagatacggt ttccattaaa atttcctccg gacaaaatta cttcacaacc
     1921 ctcatattgc tgacatttgg acgccagcta tcccagttaa acgtcaccca tcgaccaccg
     1981 ttcatggtca tgcggtccat aatcctctca ccaagaagcg tactcattgc ggcatgattc
     2041 aggtttgtta acatcccgac actgcacagt gatgctgtcc ggcgatcaat tatctggtgc
     2101 aataccacct gctcgttttt cgtctcccgc tgaacgccta tttcatccag gaccagcaaa
     2161 tcaaccccgc aaagctcctg tatttttccc cggatttgcc gttgtcgtag ctgtcatgca
     2221 acacgctcat gacgtcagac acggtgacga taatcacgct gcgccccttc accatcagcc
     2281 ggttgcccat cgccgctgca aggtgatttt tcccggtgcc ggttttaccg ctgaacacaa
     2341 aattcgtgca cccggtcatc agttcgtcag ctatggattt ggcctggctc agcgcgtatt
     2401 tttgcccgtc gttctgcacc tgataatttg caaacgagca tttgctgtgc agaggctgga
     2461 tgcccgaacg attcaggatt ttttccaccc gcaactggcg attctggcgg ttaatctcct
     2521 cgctgcgttt tcgtccttca gcaagttgcc attcccgcca ctcctccacc gtccggtacg
     2581 gtggaaccga cccctgtggt gcaagtctgc gaatacgttc aagaacccca actgccgcaa
     2641 tgtttttcat gacacgtcac cccctgaatc ccggcggtat ttcagtgtcc ggttcagaaa
     2701 tgtgattcac acaacgctgc gcaggcgaac gccccaggcg gataaccagt tcatcccatt
     2761 tttcccggag ttttgccgga ctcatgatgt tttttaccca gaacgaatcc cgctggagac
     2821 gcccaaacat ttcacaaatt tgtctgtgag ttctgccatc cagcatccgc attgtgcgaa
     2881 cgtcattggc ccatgctgtc cagttgggtt ctttcggtct agtgatctcg ccatcatagc
     2941 tggccgcctg ctcgtaaaga ctcacgattc gtccccagat ccactgtgcg cacaccaaat
     3001 cttcctgact tccccactgg cgttttttcg cactgaacac aaccgcgtca gggtgtcggg
     3061 ttaaaaaatc ctgttcagcc gtctgcgggt ccggttgcga agcgtccgga caagaagatc
     3121 ttttatctga cggatcaggt tttaatactg acggatcggg gtcaatcatc gcccccctaa
     3181 tcggcagttt tttatcaaca gttgatccat caaaatttga cgggtcaacc gttgaggggt
     3241 caatatttga cgggtcaact gttaacgggt cattttttgc cgggctaatt tttcttttcg
     3301 gtttatatga ctcacgcgcc gccgccgcag ctgcttcgag tttttccaca ttaagccgat
     3361 agatattgct tacattacgc ccaccgacct tacgctcttc cttcgtcagc cagccctctt
     3421 tcgccagttc tgcaatagcc gatttcactg tggattcact tcttgcaccg atctgacgcc
     3481 ggatagtttc aatggcaggc catgacacgc cctcgtcatt gctgtagtct gcaagacggg
     3541 ccataaccgc caccctggat aagatcatgc cggtgaaggc gcacccttcc cagacaagac
     3601 catgaagctt gctgctcata aaacccccga acaccgtgct tttagtgcat caccacagca
     3661 ttccctgccg ggccgccgcg attcatctgg tcatacaaaa caaccgctga cgcaacaaaa
     3721 tcatcgacat ccttcaccag ccgatccctc cgttcgacga tctcacggta atattcagaa
     3781 ctgtggctgc gcatacgggc caccagcaaa ggcggcatcg ccttttcgat cgccggtaac
     3841 agagcctgca ttttttcaac agcatcaggg gggtctttat ccagccaacg gaaaattttc
     3901 tgggtattac gggccagggc ttccggatgg ctgtcgtcgt acagttcagg gaacgtcatc
     3961 cccagttcga aataagtccg ggctatttca gctgcaggaa ctttctcacc atcagggtat
     4021 gcccaggcat tcatcgccat gcggatgtgc tcatgtttga ttttcatgaa tcatttgcct
     4081 cttgatgctt cgggtatgat cgttttcgtc atttggttgc ttcatcgaca tattctgcga
     4141 ataacatgac gagcgtcgta agtatgtcca atcaacatca ggacgaagtt cttcacacag
     4201 gacaccacct tttgttgctc gttcaatcgc aggacatctc tcagcaggta actgacgtac
     4261 acctttgatc cattgattta cgcttggagg agatacacct aaaagcctag ccattgctga
     4321 ttgcccaccg acaacagcac aagctcgttt gaatgaatag ttatcttttt tcatcgaatg
     4381 aactccaaaa aacacgcaac aatattaggc ttagcctaat gaaattgtca ataggctatg
     4441 cctaatacat cgagagtagg gattgcctaa cgcgatgcgc ataggagact attaagcaat
     4501 gcttagtggt aaagacttag gccgagcgat agagcaggcc attaacaaaa aaattgcatc
     4561 aggagccgtc aaatcaaagg cggaaatcgc acgtcatttc aaagtccaac caccatcaat
     4621 ccatgactgg attaagaaag gttcgataag taaagacaaa cttccagaac tatggcgttt
     4681 cttttctgat gtggttggtc cagagcattg ggggcttaac gaatacccca taccaacccc
     4741 atccacttca gatacaaaaa gtgaactttt agacataaac agcctttatc aagccgcctc
     4801 tgatgaaaaa aaagcaattg tggctttcat cttatctgga aatgctacgg agcctagttg
     4861 ggttgattat gacgttcgcg cctacattgc cgcaatggaa atgaaggtag ctaactatct
     4921 gaaaaatcaa gaatcaaaac ggaaaagcca gaacatcacc aagacaggaa cttaaactta
     4981 tatggtccga cgggaaattc ctagttcccg ttagttaact cctactacct ctcccacaaa
     5041 ccatcaccta ttaggttgcg cccaaattat taggcatagc ctattgacaa gtaattaggc
     5101 atttcctata gttttcccat accaacccat cccgtcccac acaatacagg gcaatacctc
     5161 gagttaccag gcagtggtca ggggttaagt agccagcccg aggcgtaaga acatgacggc
     5221 agggttcaac tttaataact atgcagcagg tttttgttcc gctaccccgg cgttaagggg
     5281 aaatgaggtc agcatggata ctatcgagct tggcaacagc gaatctctgg tatgtggcgt
     5341 gttccccaac caggacggta cgttcaccgc gatgacgtat accagaagca aaacgtttaa
     5401 aactgaagct ggcgcgcgtc gctggttaac cagaaacact gactgatgag gttgacgatg
     5461 gaatttaaag atttaccagt accattccag gaaatggcat cgaatgtggt tcgctctcaa
     5521 ctggcgactc ttgacctgag taccgtagaa aaagaaacca tcgacaatat atccggtaac
     5581 gtacgccgag cctttatcgg gctgtacgaa gagaagctgc tctctgataa ccaggattta
     5641 catgaaaaat acttcctgga tctaatggac atcattgata aggggtttgg cttgttaatg
     5701 aaaaagaaag ggattcgaat agaacccctt gaaaattact ttgcaacaaa aagcattaat
     5761 tcttttgatt caaaataaga gaattaatta cagacttaac atgctctttc tcatgattga
     5821 agctctcatg attgaaagtg ccgggttgaa gcgagtcgat ataatcaaca agactctgtc
     5881 gtacgacttc atttttatcc ataacagatg caagaaatga aattgctaaa agagttatat
     5941 cactacgcgc cgcagcatgt tgcaatgctt tatcaaaatt attaatctgg cgtatcaggg
     6001 agttaatgat ttcatcattt tcagtcgaca tttcaccctc ctgagggttg gtgattaagg
     6061 agttctccac gggtgaggtg gagtgcgtgc gccggacacg ggtgagcatc cggcactgac
     6121 agtttactga aaggatattt ccctgaaaag tcagaccata acgcgaaagc gcccggcgag
     6181 gtagctggtt catagatagc ctgtcgttaa attttcgtcg accgtgcgtt tccggttgtg
     6241 gcaatccgcg aaatggcgcg gcggtaagta tggcggggtt attccttccc cgttgaggac
     6301 accgagttgt caggttgacc atacgcttaa gtgacaaccc cgctgcaacg ccctctgtta
     6361 tcaattttct ggtgacgttt ggcggtatca gttttactcc gtgactgctc tgccgccctt
     6421 tttaaagtga attttgtgat gtggtgaatg cggctgagcg cacgcggaac agttaaaacc
     6481 aaaaacagtg ttatgggtgg attctctgta tccggcgtta attgttaact ggttaacgtc
     6541 acctggaggc accaggcacc gcatcacaaa attcattgtt gaggacgcga taatggaaac
     6601 gttattacca aacattaata cgtctgaagg ttgttttgaa attggtgtca ctatcagtaa
     6661 ccctgtattt actgaagatg ccattaacaa gagaaaacac gaacgggagc tattaaataa
     6721 aatatgcatt ctttcaatgc tggcccgttt acgtccgata caaaaaggat gctggcaatg
     6781 aatacagcat ttgcacttgt tctgacagtt tttcttgttt ccggagagcc agttgatatt
     6841 gcagtcagtg ttcacaggac aatgcaggag tgtgtgactg cagcaaccga acagaaaatt
     6901 cccggtaact gttacccggt cgataaagtt attcaccagg ataataacga aatcccggca
     6961 ggtctttaaa acagttccgt aataaatatc cggtttcatt cttatatgcc agcaatggca
     7021 gggatttgtt cacccttaaa tctgtaatga ggtaaaacaa aatgagtaaa gtctttattt
     7081 gcgccgccat tccggacgaa caggcaataa aggaagaagg tgccgtcgct gtagccactg
     7141 ccattgaagc cggtgatgaa cgtcgcgccc gcgcaaaatt tcactggcaa ttcctggaac
     7201 attatccggc tgctcaggac tgcgcttata aatttcttgt ctgcgaggat aaacccggta
     7261 taccccgccc tgccctcgat tcctgggatg ctgaatatat gcaggaaaac cgctgggatg
     7321 aggagtctgc ttcctttgtc ccggttgaga ctgaatcaga tccgatgaac gtcacttttg
     7381 acaagctggc ccctgaagta cagaacgctg tcatggttaa gttcgacaca tgtgaaaaca
     7441 tcaccgttga tatggttatt agcgcacagg aattgttgca ggaagacatg gcaacattcg
     7501 acggacatat cgttgaagcg ttgatgaaaa tgccagaagt taacgccatg tatccggagc
     7561 ttaagctgca tgccatcggg tgggttaagc ataaatgtaa gcctggtgcc aaatggcccg
     7621 aaattcaggc agagatgcgc atctggaaaa aacgtcgcga aggtgaacgc aaggaaaccg
     7681 gaaaatacac gtctgttgtt gatctcgccc gcgccagaac caatcaacag cacagtgaaa
     7741 attcaacagg aaaaatcagc ccggtcattg ctgccattca tcgcgaatac aagcagacat
     7801 ggaaaacact ggatgacgaa ctggcctacg ctctctggcc tggtgatgtg gatgccggaa
     7861 acattgacgg cagcatccat cgctgggcaa aaaatgaagt tatcgacaac gaccgcgaag
     7921 actggaagcg tatctcggca tcaatgcgca aacagcctga tgcccttcgc tacgaccgcc
     7981 agactatttt tggccttgtc cgtgaacgtc cgatcgacat tcacaaagac cctgtggcac
     8041 tgaacaaata cattactgaa tacctgacta caaagggcgt gtttgaagat gaaggaagaa
     8101 atcagagcgc aactgatact ctctcgtcgc cagtaccaga aactgatgca gtggaaacgg
     8161 caattccggg caacgaaaaa accgaatgca aagtggaagt cgaaccatct gtagagcgtg
     8221 aggggccgtt ctacttcctc ttcaccgaca aggatggcga aaaatacggt cgcgcaaaca
     8281 aactttctgg tctggaaaaa gcactagcct tgggagctac ggaaatcaca aaagaggaat
     8341 acttcgcacg taaaaacggc acgtactcag gttcacaaca aaatactggt gcatctgaca
     8401 cgaccgcaca accagagccg gtaaaagtta ccgctgacga agtaaacaaa attatgcagg
     8461 cagccaatat cagccagcct gacgccgata agttgcttgc tgcctctcgc ggagaatttg
     8521 ttgcagggat tagcgacccg aatgatccga aatgggtaaa ggggattgaa acccgcgatt
     8581 ctgtaaacca gaaccagcaa gaatcggaac agaacgacca gaaagcggaa caaaacagcc
     8641 caaatacgca acaaaacgag ccagaaacga aacaacctga gccagtagcg caacaggaac
     8701 cggaaaaagt ctgcaccgcc tgcggtcaaa gcggtggcgg caactgccct gattgtggcg
     8761 cggtgatggg tgacgcaaca taccaggaaa cattcgatga agagaatcag gttgaagttc
     8821 aggaaaatga tccgaaggaa atggaaggcg ctgaacatcc acacaaggag aatgctggta
     8881 gcgctcagga tcacgccagc gatagtgaaa ctggcgagac ggcagatccc ttaattgcga
     8941 tgaacggtca tcacgttatc acatccacca gcaggacgtg tgaccatcta atgatcgacc
     9001 ttgaaaccat gggaaaaaat cctgatgccc cgattatctc aataggtgca atatttttcg
     9061 atccgcaaac cggagatatg ggaccggaat ttagtaagac tatcgatctg gaaactgctg
     9121 gcggagtcat tgatcgtgac gtcattaaaa ggtggctgaa gcaatcacgt gaagcgcagt
     9181 ctgccattat gaccgatgaa atcccgttag atgatgcact gttacaattg cgggaattta
     9241 tcgacgaaaa ctccggtgaa ttttttgttc aggtctgggg aaatggagcc aacttcgaca
     9301 acacgatttt gcgccgttca tacgaacggc aggggatccc ctgcccgtgg cgttactaca
     9361 acgatcgcga tgtacgcaca atcgttgagc tggggaaagc catagacttc gatgccagaa
     9421 cggctattcc attcgaaggt gagcgccata atgcacttga tgacgcccgt taccaggcaa
     9481 aatacatttc agttatctgg caaaaactga tcccgaatca ggctgatttt taatgttcaa
     9541 ccccggtcgt tgcccaccag ctatagtggc ggcgaccatg attagcgaac gacgctcatg
     9601 gcaagactta ttctgctcac tgagtgggca aaagaggaat tcagtgaacc ggtccctact
     9661 ccgagtacgt taagtaaata cgctaaagcc ggaatgatat ttcctctccc caaaaaagtt
     9721 ggaagacgct ggcgagtgga tccgcaagct cgctttgtcg gaatggtaaa caagccggag
     9781 gtgatcgcca cagatcaccc tgctttgaag aggatactgg aagatggcgc gcccgcgaaa
     9841 atataaaacc gatgttccgg gattatctcc gtattttgac aaaagaaata acaaagttta
     9901 ctggcgttac aggcatccca taacaggcaa aaatcacggt ctcggcagta ttgaccagaa
     9961 actggcagaa actattgcag cagaagcgaa cagccgtctt gcccggcagc aaatggaaca
    10021 aatgctcagt ctgcaggaga aaattattag tgataccggc ggttcatcaa ctgttaccat
    10081 ttttctgaat aattacagaa aaattcaaca ggaaagatat gaaaacggcg agatcaaact
    10141 caacacgctg aaacagaaag cggcccctct cagggtattt gatgaacgtt ttggcaccag
    10201 acggttagat gccataaccg taaaagatgt ggtatcagta ctggaagagt acaaggccag
    10261 aggacataac agaatgggac aaattttcag gaaagtactg atcgatgttt tccgggaagc
    10321 tcagcaaacg ggcgatgtcc cgccaggctt taaccctgca gaatcggcaa aaaaaccgca
    10381 ggtgcggata tcaagacagc gactgacttt tgatgagtgg atgatgattt ataacgcagc
    10441 ggaaaaggat ggttactttt tacagcgcgg tatgctgctg gcactgatga caggccagcg
    10501 cctttcagat atttgcaaaa tgcaattttc ggatatccgg gatggttatc ttcatgtcga
    10561 acagcaaaaa acaggaaccc ggattgccat ccctctggct ctgcgttgcg ataaattaaa
    10621 tctcaccctg gatgatgtgg tgtcatcctg ccgcgattgc gttcttagtc cgtggctatt
    10681 gcaccaccat cacgcgaaag ggacagctaa gcgcggcggg atggttaagc cagcaacatt
    10741 aaccgttgca tttaaaaaag cccgggattc tgtggattac aactggcgtg ctaatggcac
    10801 ccctccctct ttccatgagc agagatcttt atcagagcga ttgttcagag agcagggggt
    10861 tgataccaaa attttgctag gccattcgaa tcaaaaaatg accgatattt acaacgacgc
    10921 acgcggtaag gaatggaaaa aactggtcat ttgatgacca gttttgcaga ggggttttgc
    10981 agaggttttg cagagaaaat taaaaacgat atcctgcgga gaacataaac acccacggat
    11041 ccagtcgtac cgagtctttt acggtagtaa caccagattt gtacttagcc gtggtatcga
    11101 tatccatgta ccagactgac atgttaacca accagtcacg gttaatcaga taatcaaccc
    11161 ccacctgccc ggcagctccc caggaatctt tcagactgag atcggaaagc cctgcctctt
    11221 tgccatgatc gttaaatcca ttatcaaaga aggtggtgta gttaatacct gccccaacgt
    11281 aaggacggaa tttgctgctg gcatcaccaa aataccactg cgccatcagt gttggcggca
    11341 gatggtgaac ggttgcaata tcgccggtcg cccgggtgcc gattttatgg cggaacggcg
    11401 tcgctgccag taattccaca ccaatgttgt cggtcgccat ataagtaaac gtcaagccca
    11461 gttgagtgtt attggtcacg ctgaatccac ccagacttcc taacgtacca ccagcacctt
    11521 ctgttggacg tacggttgca gaacctgcac gcataaaaaa ttcgcctgct tcatgcgcaa
    11581 aggcactgcc agagagaaga gttgttactg ccaaagccgc cactgttaac tttttcatat
    11641 ccgctccgtc gttatggtta taaaaacgtg acgaatatac ctatattgga gtaacaaatg
    11701 atcctacata gatcacatta catatgataa ttcacccttt attgatctgg attaatttag
    11761 aagttgcatt gagaatcctg gttaacgtga tttaaatcaa tttttaaata ttttcgctac
    11821 gcaaatatta actcgatcgc cacttgccgc ggtttgccca cgcctgaagg ccagcaatta
    11881 cctccgcttt acaaagatat gcttttccat acaagacctg atgctgccac ccgaggctta
    11941 accagggtac aattgcccgc taattaacac ctgcataaaa ctcaaggaga gtgcatgtct
    12001 atcacggcgc agtccgtata ccgtgacacc ggaaatttct tccgtaatca atttatgacc
    12061 attctgttgg tatcgttgct atgtgcgttt atcacagtgg tgttagggca tgttttctca
    12121 cccagtgatg cacagcttgc gcagctcaat gacagcgtgc ccgttagcgg cagtagtggg
    12181 ttgttcgacc tggttcagaa tatgtcaccg gaacagcaac aaattttgct gcaggcttca
    12241 gcggcgtcca ctttttcagg attaatcggt aacgccattc tcgccggagg cgtaatatta
    12301 attatccagc tggtgtctgc gggtcagaga gtcagtgcgc tgcgagctat tggtgccagt
    12361 gcaccgatat tgccaaagtt atttattctg atttttctga ctaccctttt agtacagatt
    12421 ggcatcatgc tagtggtcgt tccgggaatt atcatggcca ttttactggc cctggcaccg
    12481 gtgatgctgg tacaggacaa aatgggcatt tttgcctcga tgcgtagcag tatgcggctg
    12541 acttgggcga atatgcgtct ggtggcaccc gcagtactga gctggttgct ggcaaaaaca
    12601 ctgttgctgc tttttgcctc ttcttttgcc gcattaaccc cggaaattgg tgccgtactg
    12661 gcgaacacct tgagcaacct gatttcagcc gtattgctca tctatctgtt ccgtctgtat
    12721 atgttgattc gccaataacc ttaagcattt gattacggaa tcgtaaaatg aagcagtttc
    12781 ttgatttttt accgctggtt gtctttttcg cgttttacaa gatttatgac atctatgcgg
    12841 ctactgcggc gctgatcgtc gccacggcga ttgtgcttat ctatagctgg gttcgctttc
    12901 gtaaggttga gaagatggcc ctgatcactt ttgttctggt ggtcgtcttc ggtggcttga
    12961 cgctgttctt ccacaatgat gagtttatta aatggaaggt tacagtcatt tatgctctgt
    13021 ttgcgggagc cctgttagtc agccaatggg tgatgaaaaa gccgctaatt cagcggatgc
    13081 tgggtaaaga actcacgctg ccgcaaccgg tatggtcgaa gctgaatctg gcctgggctg
    13141 ttttctttat cctttgcggt ctggcaaaca tctacatcgc attctggctg ccgcaaaata
    13201 tttgggtcaa ctttaaagtc tttggactga ccgcccttac cttaatcttc acattgttaa
    13261 gcggtatcta tatctaccgc cacatgccgc aggaagataa atcctaacca gactgccagt
    13321 ccttcggggc tggcgctgta tctcctctgt tctcctcagg aaaatcatag tagcatcgcg
    13381 cctgtgattt tccttttaag tcggttttac catgtctaca acacataacg tccctcaggg
    13441 cgatcttgtt ttacgtactt tagccatgcc cgccgatacc aatgccaatg gtgacatctt
    13501 tggtggttgg ttaatgtcac aaatggatat tggcggcgct attctggcga aagaaattgc
    13561 ccacggtcgc gtagtgaccg tgcgggttga aggaatgact ttcttacggc cggttgcggt
    13621 cggcgatgtg gtgtgctgct atgcacgctg tgtccagaaa gggacgacat cggtcagcat
    13681 taatattgaa gtgtgggtga aaaaagtagc gtctgaacca atcgggcaac gctataaagc
    13741 gacagaagca ttatttaagt atgtcgcggt tgatcctgaa ggaaaacctc gcgccttacc
    13801 tgttgagtaa tagtcaaaag cctccggtcg gaggcttttg actttctgct tactgaattt
    13861 cggtggtgcc gttaatttta aacaggatat tcaccacaat cccactgcct ggcttacccg
    13921 gctcataacg ccatctgcgc atcgcatttt tcacctcacg ctcaaacatg ttcgcaggct
    13981 tggctgagag gatttgtacg ttatccacgc gaccatccgg cgtgacgtca aatttaactt
    14041 taacctgccc ttcaatgcgc aatgcctgtg ctcgtgccgg atactgcggc tgattacggc
    14101 ttaatgcgcg tggtcctgaa gccacactgg taaccggctt gctggttgca gccgttgctg
    14161 tacttgatgt caggcgtgcc agtgccgtat tttcaaacgg tgatgccgga cgcgactcta
    14221 cgggtttgac atcgcgtttt ggctgctcct gtaccttttt caccggcttc ggttttggct
    14281 taggtttcgg cttcggcttt tcaatgacca ccggtgcttc ttttggcggt tcggggatcg
    14341 gctcaggttc cggttctggc tctaccaccg gctccggtgg cggctgaacg gcttgtggcg
    14401 gttcgagatc agcaggcgca accatcgtga cagaaattgg ctgcgcaggc gcaggtagtt
    14461 caataacctg atgtaccgag gtatagagca gacccgccac aacagcacca tgaatgcaga
    14521 ccgaaagtaa cgtcggccag gggaagcggc gaggtaaatc aagggtcatt gaagtcataa
    14581 tcatttcagt agaaaaacca agcctcgatt ttaaatgcaa atagcaatca tattcaataa
    14641 ggcaagacga tcaggatcag tgaaaatgtc atttttgccg taattaacct tactttatgc
    14701 atggtcttaa caaagacatc atctttccgt tgcagatctt cgccctttca aataacgtac
    14761 tttacaactt tcccgaacaa ggagttgtgc ccgtgttgta tgttatttac gctcaagaca
    14821 aagctgatag cctcgaaaaa cgcctttccg ttcgtccggc acatttagca cgtttacagt
    14881 tactgcatga tgaaggtcgt ttgttgacag ctggtccaat gccagcggta gacagtaacg
    14941 atccaggggc tgcgggattt actggctcaa ccgttattgc tgaatttgaa tcgctggaag
    15001 cagctcaggc ctgggccgat gctgacccgt acgtagcggc aggagtctat gaacacgttt
    15061 cagtgaaacc atttaagaaa gtgttctgaa tagagattca atatttaagg gcacggtttt
    15121 tgcaaccgtg ttcttgtttg gcctttcctt atagccggtc agtgcattca ccaccacaag
    15181 cagataataa ctttatctgt atttctttat atgatgtgag catattcagg aaaaatgacc
    15241 tttcctgagg tacagttgct cgctcaaaac ctttgattac tgcggataaa gtcatctact
    15301 attgtttaac acattcacgc gctgcatgta tgttcagaaa atgtgcacgc taagataatt
    15361 aaacaaaatt gtggttacag aggtatcagt gagtcactgg gctacattca aacaaacagc
    15421 aacaaattta tgggtgaccc tacggcacga cattctcgcg ctggccgtct ttttaaatgg
    15481 attgcttatt tttaaaacaa tctatggtat gtcggtcaat ttgctagata ttttccatat
    15541 caaagcattt tcagagttgg atctttcctt gctggcaaac gccccactat ttatgctcgg
    15601 cgtctttctt gtcctgaact ccattggctt actgttccgg gcaaagctcg catgggcaat
    15661 cagtatcatt ttgttgttga tagcgctaat ttataccctg catttttatc cctggttgaa
    15721 atttagtatt ggattttgca tttttacgct ggtgtttttg ctgatactgc gcaaagactt
    15781 ctcccacagt agcgccgcag ccgggacaat ttttgcattt attagtttca cgacattact
    15841 gttttactca acctacggtg cgctttattt aagcgaaggt tttaatccgc gaatagaaag
    15901 tttgatgacc gcgttctatt tttcgataga aaccatgtca accgtcggct atggcgatat
    15961 tgtccctgtt tctgaatcag cacgattgtt cactatttcg gtcattattt ccggtattac
    16021 cgtttttgcc acatctatga cctcaatttt tggtccgctt atccgcgggg gattcaacaa
    16081 acttgtaaaa ggaaacaatc atacaatgca tcgtaaagat cattttattg tttgcggaca
    16141 ttcgattctc gccatcaata cgattctgca actgaatcaa cgcggacaaa acgtaacggt
    16201 tatcagcaac ttgcctgaag atgatatcaa gcaacttgag caacgcttag gcgataacgc
    16261 tgatgttatc cccggtgaca gtaatgacag ttcagtatta aagaaagcgg gaatcgatcg
    16321 ttgcctggcc attctggcgc tgagtgataa cgatgcagat aacgcgtttg ttgtactctc
    16381 ggcaaaagat atgagcagtg atgtcaaaac agttctcgcc gtcagtgata gcaaaaacct
    16441 gaataagatt aagatggtac atccggatat cattctctca ccgcaactgt ttggcagcga
    16501 aattctggcg cgagttttaa atggtgaaga gattaataat gatatgctcg tttcaatgtt
    16561 gttgaactcc ggtcatggca ttttcagcga taacgatgaa caagaaacga aagctgacag
    16621 taaagaatcg gcgcaaaaat agagtttaaa gatggaggag taaacttatt gggcaccgca
    16681 atggtgccca gtacatttag atattgtaga tcgcaaacaa taacgagtta cgctgtttgt
    16741 ttagaatgca cttcctgatg ctgtggatac gcatatttcg gcgcgattgc ccctcaagcc
    16801 aacgcgattt acgtcggcta gcctgacgct gcatgcgcca gcgccccact tccgttctac
    16861 tccgcttcat gttaactact ctatgcaata acagaatggt cattataccc ccgcccccga
    16921 cgctgaccag tgcttttccc gcgtttttat ttgccagatg aatccatatg cgtaaactca
    16981 taacaatgcg ctttcaaaag gatttctaat ctatgacaac cgtttatacg ttggtgagtt
    17041 ggttggccat tctgggatac tggttgctca ttgcaggcgt aactttacgc attctaatga
    17101 aacgacgcgc agttccctcc gcgatggcct ggctgttgat tatttacatt ctgccgttag
    17161 tcggaattat tgcctatctt gccgttggcg agctccattt aggcaaacgc cgcgctgagc
    17221 gcgccagagc gatgtggcct tccaccgcaa aatggcttaa cgaccttaaa gcctgtaagc
    17281 atatcttcgc cgaagaaaat agcagtgtcg ctgcgccatt attcaagctt tgcgagcgtc
    17341 gtcaggggat cgctggggtc aaagggaatc agctacaact gatgaccgag tcagatgatg
    17401 tgatgcaggc gttaatccgc gacatccagc tcgcgcgcca taatattgag attgtgtttt
    17461 atatctggca gcccggcggc atggcggacc aggtggctga atcattaatg gccgctgcgc
    17521 gacgcggcat tcattgccga ttgatgctcg actccgccgg gagtgtggct tttttccgca
    17581 gcccgtggcc cgagctaatg cgtaatgccg gtattgaagt ggtcgaagcc ttaaaggtca
    17641 atctgatgcg tgtgttttta cgccgtatgg acctgcgcca acatcgcaag atgatcatga
    17701 tcgataatta catcgcgtac accggcagca tgaatatggt cgatcctcgc tacttcaaac
    17761 aagatgcggg cgtagggcaa tggattgatc tgatggcgcg tatggaaggc cccatcgcca
    17821 ccgcgatggg gattatttat tcctgcgact gggagattga aaccggaaaa cgtattctgc
    17881 cgccaccacc agatgtcaat attatgccgt ttgaacaggc cagcggtcac accattcaca
    17941 caattgcttc tggccccggc tttccggaag atctcattca ccaggcatta ttgactgcgg
    18001 cttattcggc gcgtgaatat ttgatcatga ccacgcccta ctttgtgcca agcgatgatt
    18061 tacttcatgc gatttgcacg gcggcacagc gcggggtgga tgtcagtatt atccttccgc
    18121 gaaaaaatga ctcgatgctg gtcggctggg ccagtcgcgc attctttacg gaacttcttg
    18181 ctgctggggt taaaatttat cagtttgaag gcgggttact gcataccaag agcgtgctgg
    18241 tcgatggcga actaagtctg gttggcaccg ttaaccttga tatgcgtagt ctgtggctga
    18301 atttcgagat taccctggca atcgacgata aaggttttgg tgcagacctt gccgccgttc
    18361 aggacgatta tatttcgcgt tcacgtctgc tcgatgcccg tttatggcta aaacgtccat
    18421 tatggcaacg tgtcgccgag cgactgtttt acttcttcag cccgttgctg taaaacgtct
    18481 ccaacagagg ttaaacaggt agtgactatg gatatggatc taaacaatcg cctgactgaa
    18541 gatgaaacgc ttgagcaggc ctacgatatt tttctcgaac tggctgccga caatctcgac
    18601 ccagctgatg ttctgctgtt caatcttcag tttgaagagc gcggcggcgc ggagttattc
    18661 gatccggtgg aagactggca ggaacatgtt gatttcgatt tgaacccgga cttttttgcc
    18721 gaagtggtga ttggtctggc ggacagtgag gatggtgaaa tcaacgatgt tttcgcccgc
    18781 attctgttat gccgcgaaaa agatcacaaa ctttgccata tcatctggcg ggaataaata
    18841 atcgctttcg gcgtgaaaaa gggctgacaa ctgtcagccc ttattgtgtt taaagcggat
    18901 cgactttcag gcaagaaacg gcgtgtctga aactcccttc cagcacagga cgtgttttgg
    18961 cgcactccgg accggcaatc gggcaacggg tacggaaaac acaaccggaa ggcgggttga
    19021 tcggcgacgg taattcccct tccagtaact ggatggtttt gttcttctcc agatccggat
    19081 caggtatggg gactgccgac atcaatgccc tggtgtaagg atgtagtgga ttgtggtaga
    19141 cctcatcata ggtccccagt tctaccgcat ggccgagata catcaccaac acacgatcgg
    19201 aaatgtgttt taccacggcc aggtcatgag caataaaaat taatgacaat cccatctcac
    19261 gttgcagctg ctggagcagg ttgaccacct gcgcctgaat tgacacgtcc agcgccgaca
    19321 ccggctcatc acagataatc agcttcggtt caagaataag agcacgagca atcccgatac
    19381 gctggcactg cccaccagag aactcatgcg gatagcggtt aatcaggtta ggcaataacc
    19441 cgactttcag catcatcgcc ttcacgcgct cgcgaacttc ctgacgtgac attttcggat
    19501 gataagtacg cagtggttca gcgatgatct cgccgatggt catgcgcggg tttagcgatg
    19561 ccaacggatc ctggaaaatc atctgaatat cactgcgaac ggcacgccat tcatcgggct
    19621 taatgcccag caactcttta cctaaccagg caacatgacc gtcggtcgct ttaaccaggc
    19681 caatgatggc gcgagcaaag gtggacttac cgcatcccga ttcccctacc acacctaatg
    19741 tttccccttc atacaggcga agcgtgacac catcgacggc tttgagcgtt ttcggcggtt
    19801 gccagaacca ctgtttgcca tctttgattt caaagtgcac tttcagatcg gcgatttcaa
    19861 ggaggacttt tcttccttcg gttacagcat tcataacagt tcctccaccg gtttaaagca
    19921 agcacacaga cggccaagcg taaactcttc cagcggcggt gcgctactac aaatttccat
    19981 cgcatgcgga caacgtggct ggaacgggca acctttcggt aatcgcagca ggtttggcgg
    20041 attacccggg atggtcaaca ttgtttcacc ttccgcatcg agacgcggca ccgcgttgag
    20101 caaaccgata gaataaggat gaacgggttg ataaaagaca tcgcgcgcgt tgccatattc
    20161 catcgtgcgc ccggcgtaca ttaccagcac tttgtcacag atccccgcca ccacgccaag
    20221 atcgtgggta atcataatga tggcggtatt aaattcccgt ttcagttcat ttaatagcgt
    20281 catgatctgc gcctgtacgg tgacgtccag cgctgtagtg ggttcatccg caatcagcag
    20341 cttaggtcga catagcaatg ccatcgcaat cataactcgc tgacgcatgc cgccagaaaa
    20401 ctcgtgcggg tacattttca tgcgtttacg tgcttccggc atttttaccg catcgagcat
    20461 ccgcaccgac tcttcaaacg cctcagcttt gctcatgttc ttatgcagca tcagcacttc
    20521 catcaactgc tcaccgacgc gcatataggg attcaacgaa gtcattgggt cctgaaaaat
    20581 cattgagatt tgttcagcgc gcagtttatt gagttcatgc tctggcaaat tgaggatttc
    20641 acgcccattg aaggttgccg atccgccaat acgtccgttg gcagccaaca ggcccatcaa
    20701 cgcaaatgca gtttgcgatt tacccgaacc agactcacct acaataccca gcgtttctcc
    20761 ggcacgtagg gaaaaattca aatcattgac cgtcgtgacg tcgccgtccg gggtactaaa
    20821 ggtgacacgc aaatctttca cgttcagcag tgcgtcagcc tgttgttgtg cgagcggcac
    20881 agttgcagtt tcaattacgc tcatggctgc actccttaac gatctttcgg gtcgagggca
    20941 tcacgcaagc catcgccgat aaagttgaaa caaaacagcg tcaccacgag gaatcccgct
    21001 gggaacaaca ataaccatgg agagacttcc atcgagttcg cgccatcact cagcaatgca
    21061 ccccagctgc ttaacggctc ttgcgtaccc aaccccagga agctaaggaa agattcaaag
    21121 aggatcatgc tgggcaccag aagtgatgcg tagaccacca ccacaccgag tacattcggc
    21181 acaatgtggc gaataacaat gcccgatgtc gatacaccgc caacttgtgc cgcctcaata
    21241 aactctttgc gcttcagact cagggtttgc ccacgcacaa tacgagccat atccagccag
    21301 gaaaccatgc ctatcgccac gaaaatcagc aggatgtttt gaccgaaaaa ggtcaccagc
    21361 aaaatgacga agaacatgaa tgggaaggag ttgaggattt ccagcagacg catcattacc
    21421 gaatccactt taccgcccag ataaccggaa agcgaaccat aaagtgtccc gacgaccaca
    21481 gccaccagtg ccgcagcaac accgaccatg agtgagatac gcccaccaat cgcaacgcgc
    21541 acaagcaggt cgcgaccgga tgagtcagta ccaaagtagt gaccggactc catatccggg
    21601 gcgctggaca tcatcgccca gtcagtatcg tcataggcaa actgggaaag catcggtgcc
    21661 aggattacaa ataacgcgat cagcaccagc accatcagac tggcaaccgc cgcacggtta
    21721 tgaataaaac gtcgacgtgc gtcctgccac aagctgcgcc cttcgacctc cagcttttca
    21781 ctgaaatttt ccagcgtctc gctgtttttc ttacttaaca tcatcgcgag ctccagtatc
    21841 agtaacggat tttcgggtcg ataaccgcat atagcacatc gacaatggca ttaaacaaaa
    21901 tggttaaagc accaaccagg atggtcaggc ttaacactaa ggaatagtca cggttcaatg
    21961 cgccattaac gaacagttgt ccaatccccg gcaaaccata aatggtttcg ataaccatag
    22021 aaccggtaat aatgccgaca aatgcggggc ccatataaga gagcacgggt aacagagcag
    22081 gttttaatgc gtgacgtaaa atgatccgcc gcataggtaa ccctttcgcc cgggcagtac
    22141 gaataaaatt ggagtgtaat acttcaatca tagagccacg ggtaatacgc gcaatactgg
    22201 cgatataagc gagtgacaac gccaccatcg gcaatatcat gaatttaagc gccccaccat
    22261 tccagccacc gcccggcagc caatgcaaaa tgatcgcaaa tatcatgact aataatggtg
    22321 caaccacaaa actggggata acaaccccgg tcattgccag ccccatcacg gtatagtccc
    22381 atttggtgtt ttgttttaat gcggcaataa cgccagcact aacacccagt attaccgcaa
    22441 ggaaaaatgc tgcggctccc agtttggcag aaacgggaaa actggatgca accaagtcat
    22501 tgaccgaata atctttatat ttaaacgatg gaccgaaatc accgtgcgcc agttgtttca
    22561 ggtagctgaa atactgtgtc atgatcggat cattaagatg atatttcgct tcgatattgg
    22621 ccatcacttc tggcggtaaa gtacgttcgc cggtaaaagg gcttcccggc gcgaggcgca
    22681 tcataaagaa cgaaatagta ataagaataa atagcgtcgg aatcgcttcc agacagcgac
    22741 gtagaataaa ttttaacatt gccctacctt ctggcctgtg ccttctgtaa tgcgataaaa
    22801 atcagacacc gtggagcagg acactcctgc cccacgtatt gccattagtg cttcacaatg
    22861 tacatattcc gggtataggt attatccagc ggatctttgc cggtatagcc accaacccac
    22921 ggtttcacca gacgcgcatt cacgtagtaa taaacaggaa caatggccga atccttatcc
    22981 agctgttgtt ctgctttagt gtacagagct gtgcgctgcg cctcgtcagt cactttcagc
    23041 gtttccgcca taatgctgtc aaaggccggg ctcttataat gcgcggtatt catcgagctg
    23101 ttcgaaagca tggtgttcag gaaggaagtt ggttcgttgt agtcagcaca ccagcctgca
    23161 cgggccacat caaaagtacc ctggtgacgg gtgtcgagga acgttttcca ctcctggtta
    23221 accagtttga cgtttacacc aatgtttttc ttccacaatg aagaggcagc aatcgccagc
    23281 tttttatgca gatcggaggt gttatacaac aggttgattg tcaacggttt gtctgcggta
    23341 taacccgctt cagccagcag ttttttcgct tcttcgttac gtttttcctg gctccagcca
    23401 aaccattccg gctgagtcaa ttttgcgcca tcagtatacg gtggagtgta accataggcg
    23461 ggcatgtcgc cctgcgcttt cactttatta acaatgatat cgcggtccat acctagtttc
    23521 agtgcggtac gcacacgcac atcgttgaat ggcggtttct ggttgttaat ttcgtaatag
    23581 taagtgcaca ggtatggatc aacgtgaact tcgtccggga tctctttttt cagcttctgg
    23641 aacaattcga tcggcatgtt gttataagtc atgtcgattt caccactacg gtagcggttg
    23701 acatcggtaa cttcagaagc aataggcaaa taggttacct ggttaataac ggttttcgcg
    23761 ttgttccagt aggtcgggct gcgttcaaga acgattcgtt cgtttacgac ccaatctttt
    23821 aaggtatagg caccgttggt gacgatatta ccaggctggg tccatttttc gccgaatttc
    23881 tcgatagcgg cttttggcac cggtgaagtt gatgggtgaa caagtaattt atagaagtac
    23941 ggaacgggtt cacttaaggt gacttctaat gtgtgatcat caatagcttt cacaccgaga
    24001 tcggtaatcg gttttttccc ttcaagaatt tcatcaatac cggcgatatg cccatattgc
    24061 agataactgg catacggaga agcagtgttc ggatcaacag aacgttgcca gctatacaca
    24121 aagtcttgtg ctgtgactgg cgtaccatca gaccatttcg catctttacg caaatggaag
    24181 gtccagactt tcgcgtcttt attatcccag gattcagcga cgccaggtgc cggatgaccg
    24241 tcaagatcgc tgaccagtaa gccttcaaac aggtctcggc tgatattaga ctccggaaca
    24301 ccttcaattt tgtgcggatc taatgactga acttctgaac cattgttacg taccagcgtt
    24361 tgtttttccg ccagtgtgac gcctgcgggt acatcagctg ccagcgcgac attccctgcc
    24421 attagcgcag ccagaacgcc agctgctact aaacttctct tggtgatgtt ggtcattgtt
    24481 ttttggactc cctcattata attactggta ttaaccagca tgtgtaatcc cggattggga
    24541 ccctgcacag gctcggagac tttctgctgt caggttactt tatcgttacg gattgctatc
    24601 accgacttta tttattctgg tgattcagac gatcacctta tgtcgattcg tactgtgtct
    24661 cccctgtcga gacacattat tctttcgcga cgctctttaa ttgttaattg ttaattattc
    24721 tcattaccaa taaagaatcg tctggcggtt gcccggaaag tactaaatgc ggttcttact
    24781 cgccaataca ttttgcaaaa ttgttaagca attctctttt attgaccgta tcagaatcta
    24841 tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag attggctaag
    24901 catgtctatc ttttctgatt acagtttcag tattgaaatg cacaataatt taacaattga
    24961 ttattattta gcactttcct ctaccaaagg cagtggaatt actaatatca tttcaattat
    25021 tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatggtgag gagaagcagt
    25081 taagaaaatc cttataacta actgtaattt gagaa
//
LOCUS       DAJVOY010000044        24844 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVOY010000044 DAJVOY010000000
VERSION     DAJVOY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.044"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..337)
                     /locus_tag="OQL90_001610"
     CDS             complement(<1..337)
                     /locus_tag="OQL90_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368429.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAA"
     gene            complement(453..1004)
                     /gene="maa"
                     /locus_tag="OQL90_001611"
     CDS             complement(453..1004)
                     /gene="maa"
                     /locus_tag="OQL90_001611"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS1368430.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1176..1394)
                     /gene="hha"
                     /locus_tag="OQL90_001612"
     CDS             complement(1176..1394)
                     /gene="hha"
                     /locus_tag="OQL90_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1368431.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1420..1794)
                     /gene="tomB"
                     /locus_tag="OQL90_001613"
     CDS             complement(1420..1794)
                     /gene="tomB"
                     /locus_tag="OQL90_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1368432.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2340..5489)
                     /locus_tag="OQL90_001614"
     CDS             complement(2340..5489)
                     /locus_tag="OQL90_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1368433.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5512..6705)
                     /gene="acrA"
                     /locus_tag="OQL90_001615"
     CDS             complement(5512..6705)
                     /gene="acrA"
                     /locus_tag="OQL90_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1368434.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6847..7494
                     /gene="acrR"
                     /locus_tag="OQL90_001616"
     CDS             6847..7494
                     /gene="acrR"
                     /locus_tag="OQL90_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1368435.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7622..10984
                     /gene="mscK"
                     /locus_tag="OQL90_001617"
     CDS             7622..10984
                     /gene="mscK"
                     /locus_tag="OQL90_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1368436.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11196..11357)
                     /gene="rsmS"
                     /locus_tag="OQL90_001618"
     CDS             complement(11196..11357)
                     /gene="rsmS"
                     /locus_tag="OQL90_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1368437.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11371..11898)
                     /gene="priC"
                     /locus_tag="OQL90_001619"
     CDS             complement(11371..11898)
                     /gene="priC"
                     /locus_tag="OQL90_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1368438.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            11968..12345
                     /locus_tag="OQL90_001620"
     CDS             11968..12345
                     /locus_tag="OQL90_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1368439.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12498..13049
                     /gene="apt"
                     /locus_tag="OQL90_001621"
     CDS             12498..13049
                     /gene="apt"
                     /locus_tag="OQL90_001621"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1368440.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13178..15049
                     /gene="dnaX"
                     /locus_tag="OQL90_001622"
     CDS             13178..15049
                     /gene="dnaX"
                     /locus_tag="OQL90_001622"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1368441.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     misc_feature    14442..14506
                     /gene="dnaX"
                     /locus_tag="OQL90_001622"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15102..15431
                     /locus_tag="OQL90_001623"
     CDS             15102..15431
                     /locus_tag="OQL90_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1368442.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15431..16036
                     /gene="recR"
                     /locus_tag="OQL90_001624"
     CDS             15431..16036
                     /gene="recR"
                     /locus_tag="OQL90_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1368443.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16146..18020
                     /gene="htpG"
                     /locus_tag="OQL90_001625"
     CDS             16146..18020
                     /gene="htpG"
                     /locus_tag="OQL90_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1368444.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18201..18845
                     /gene="adk"
                     /locus_tag="OQL90_001626"
     CDS             18201..18845
                     /gene="adk"
                     /locus_tag="OQL90_001626"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1368445.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            18977..19939
                     /gene="hemH"
                     /locus_tag="OQL90_001627"
     CDS             18977..19939
                     /gene="hemH"
                     /locus_tag="OQL90_001627"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1368446.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19936..20895)
                     /gene="aes"
                     /locus_tag="OQL90_001628"
     CDS             complement(19936..20895)
                     /gene="aes"
                     /locus_tag="OQL90_001628"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS1368447.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21047..22351
                     /gene="gsk"
                     /locus_tag="OQL90_001629"
     CDS             21047..22351
                     /gene="gsk"
                     /locus_tag="OQL90_001629"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1368448.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22484..24160)
                     /locus_tag="OQL90_001630"
     CDS             complement(22484..24160)
                     /locus_tag="OQL90_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1368449.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24398..>24844)
                     /gene="fsr"
                     /locus_tag="OQL90_001631"
     CDS             complement(24398..>24844)
                     /gene="fsr"
                     /locus_tag="OQL90_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1368450.1"
                     /translation="GLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGRKYVIWGSILG
                     VAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIGMVSGLFFGFAF
                     GMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6203 a         6481 c         6383 g         5777 t
ORIGIN      
        1 gcgcggcagg tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg cggaaatcca
       61 gcacgtcgat atcttcatag cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa
      121 gcaggataaa taatacaacc agtagccaga cagagcctga cagcgtttcg gactgcaaca
      181 tcaccgccag ggtggcaaaa aaagcaacgt acataccgat aaacagcccc ggatgtttac
      241 ggataaaact gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta ttcagagact
      301 caatcgtttc ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca
      361 actttccagg gaacacccta ttttaacgtg ggaatagtag aggggaagta acagatctac
      421 cacaattttg cataacagtt gcgaaaaacc gattacaatt ttttaattat tctggctgga
      481 ttaccgccca cgacaacgtt gtccgggaca ccttttgtga caactgcacc tgaagctacc
      541 acgacgttat caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc aatccagacg
      601 ttattaccga tggtgacggg tttccccagt tcagcaccgc tattacgtgc tacagggtcg
      661 atgggatgtg ttgccgtgta gatatgaacg cctggtgcca acatacagtt atcaccgatg
      721 cgaatagggc agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt attgccgaga
      781 aaaatgttat agccataatc acagcgaaac gttggctcaa tataagcctc tgtcacctga
      841 ccgaataaat cagcgagaat ttgctggcgt aatgtgtgct cttccgccag ggaatgattg
      901 tatcggtgaa taagctgacg agcgcgcagg cgatcgcgag ataacgtctc atctgccgag
      961 cgatacaact caccagcaat catcttttct ttttctgtgc tcattactga cctccggttg
     1021 ctgaaaatgc ggcaacacag taagacgaaa agtcagtgaa cagaacgttc ccattacatc
     1081 tgtgtgataa agatcacata gggtatgtct tcatggtgaa aaagtataaa attcttaata
     1141 aacagccggt tatagctccg aaagcgaatt atttattagc gaataaattt ccatactgag
     1201 gaagggatct tgtcgtatag tttattcatg gtcaattcgg cgaggcggtg atctgcggct
     1261 gagtaaaata ccgccagttc attatctgat aattcgtatt tatttttctc gataacacgc
     1321 tccagcgtgt caattgtctg gcaacgacgt aaacgcatta aataatcggt tttcgttaaa
     1381 ggtttttcgg acatacttct acccatggtt gtaataattc taacaagata aactcgcagg
     1441 attctctttc gtcgcattga caaaacaacg gaatagtcga ttacctgact tccgccattt
     1501 ctgaagatcc tgcatattaa taccataact actgaacaac ataaaggtgt catccagata
     1561 ttcgtcgatc tgctcaatga gcttattgtc ttcattatac ttaattttgt aattaagtgc
     1621 gaaggtcgca atatgctcaa tcagttcatt caactggagg ttgatcgccg aggttgggtc
     1681 gttaacccag ccataattgc tttcttcaag gtttgcaagg cagtcatgat acagggtttc
     1741 acagagaaac ttaagctgtg cgatatcatg tcttttgggt gagtattcat ccataacgcg
     1801 tccccttctt agcggttgaa ctaacggaca cctttcggga tggaaaaaac ttactgacct
     1861 ggacttgccc tctcgtttgt tagcttaact ataagccact ctttgcaggt attcatcgca
     1921 ttattacgaa aaattacaat taggaaggaa tcgggacagg actcactttt tcatactagc
     1981 actaacccgc agcaggtaaa agcagtttgc tcgaactgtt cataacattt caggattatc
     2041 gccagatgaa attggtgaca ctaataccag gattgctctg aatatgacgt aataaccgag
     2101 gaatgaataa agaattaccg caaataatta agaatagcct ctaaatgatt atggattatt
     2161 aaatctatta gcgctactta atataaatta actaacaatc agtatttttt atgaatttta
     2221 tccgtggtta atactggttt tcgtatgaga tcctgagttg gtggttcaat tactccttaa
     2281 tgttcgtagg ttatgcataa aaaaggccgc ttgcgcggcc ttagtgatta cacgttgtat
     2341 caatgatgat cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg
     2401 cgaaccacca caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc
     2461 ccgcccatta caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata
     2521 accagcggca taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc
     2581 atccgcactg catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct
     2641 ttggcgaatt cgacgataag tatcgcgttc ttcgccgaca acccaatggt tgtgagcagg
     2701 cctacttgga agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg
     2761 ataaccccca gcggaacgac cagcataacg gagaacggaa tcgaccagct ctcgtacagc
     2821 gccgctagac acaggaatac gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg
     2881 ccggagaggc gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggcagt
     2941 ttgctcgcca gttgttccat tagctccatt gcttcaccgg tacttttacc cggtgccgcc
     3001 tggcctaaga tttccatgga tggcaggcca ttgtaacgtt ccagacgcgg cgaaccgtac
     3061 tcccaacgag aagaggagaa tgccgagaac ggcaccatct gaccatcagc agcacgaaca
     3121 taccagtcgc cgatatcatc cggcagcata cggtatttcg cttctgacat gacgtaaact
     3181 ttcttcacac gaccgcggtc gataaagtcg ttcacatagc taccgcccca tgcagcgccc
     3241 agagtggtgt taatgtcgtt gatagaaaca cccagcgcct gcgctttttc ctggtcgata
     3301 tcaatcttaa actgcggggt atcttccaga ccgtttggac gtacgctggt caacatatca
     3361 ggatgctttg ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt ttcgtgacca
     3421 aggccagcct ggtcaatcag ctcaaagtca aagccggttg cagtacccag ttccacgatt
     3481 gcgggcaggt taaaggcgaa aaccatcgca tctttgattt gcgagaaagc gcgtgttgca
     3541 cgcatggtaa tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag
     3601 gaaacgaacg caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg
     3661 aacaccgact caacgttgtt cttttctttg gtcagatagt aatgcgttac ctcattgagc
     3721 actttctgcg tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaatacg
     3781 ccctggtcct catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg
     3841 accacgatga tcagatacag caccaggtaa cgccccgtac tgcgcagaat accgcctacg
     3901 ctgtcggtgt agtggtgcgt gctcttctcg aacatgcggt taaaccagcc gaagaagcct
     3961 tttttacctt ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagcc
     4021 ggagtcagga tcaacgctac cagtaccgac agcgccattg ctgaaacaat ggtaatagag
     4081 aactgacgat agatagcacc agtagaaccg ccaaagaagg ccatcggtac gaataccgct
     4141 gacagtacca tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta
     4201 gcttcttttg gcggcaaacc ttcttccgcc ataacacgct ccacgttttc taccacaacg
     4261 atagcgtcat ccaccaacaa gccgatggcg agcaccatcc cgaacattgt tagcgtgttt
     4321 atcgagaagc caaaggcggc aaggacggca aaggtcccga gcaataccac cggtacggca
     4381 atggtcggaa tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg
     4441 aggatgatcg cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggc
     4501 gtggtgtcgt acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc
     4561 agttcagcac ggattgccgc agcggtatcc agcgcatttg caccggtcgc cagcttgatc
     4621 cccagaccgg aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc
     4681 agctcaatct tcgccacatc acgcagcagg acgcgggaac catcctgatt cactttcagc
     4741 aggattttgc cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta
     4801 agctgttggc ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc
     4861 tgcgctttga tggcggtaat gacatcaacc ggcgttagct ggaatttgtt cagctcattc
     4921 gggttcatcc agatacgcat cgcgtactgt gaaccgaaca actgaacatc acccacgccc
     4981 gacgtacggc tgatggcatc tttcatattc gccgctacgt agtcagagat atcctcctgc
     5041 gtcatggtgc catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc
     5101 tcaacgctca ccccttgctg ctgaacttct tgcggcagca acggcatcgc cagctgcagt
     5161 ttgttctgca cctgaacctg cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg
     5221 atctgcacgg tccccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg
     5281 ttcatattct gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg
     5341 gggtaggagg cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc
     5401 agtttgagga tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa
     5461 atcgggcgat cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt
     5521 ggactgttca ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg
     5581 tgcttttacc tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc
     5641 tgctttcaga ccttctgtca ccagccactt atcgccaata gcctggcttg caacgatcgg
     5701 acgggtttcc actttgtcat ccgcgccaac taccagtacg gtggcatcac cacgcggcgt
     5761 acgggttacg ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg
     5821 tgcgcgcacg aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag
     5881 ggtgatagac ccagtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg
     5941 cgggaactta atgccgtcac tggtgatcag cgacactttg gctttgccgt tctcttgttt
     6001 cagcgtgcca ttcgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac
     6061 atcaacgtag atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg
     6121 taccaatgcg ccttccgtca cgttcgactt accaatgcga ccgctaatcg gagcggtgac
     6181 tttggtgtaa gccagattga tccgcgcagt ttcaacggca gctttcgccg cagttaccgc
     6241 agcattcgcc tgttgcgcat cagccagagc ctgatcgtac tcttgcttac tgatgtactg
     6301 agtaccgagc agtttctgat aacgattcac cgtcaattgc gcgatattgg ctgcagcctg
     6361 ggctttcgcc agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat
     6421 ctgatagaga gagacaccag cttcgatgtc gctaccttct ttgaaattac gcttcaggat
     6481 aatcccgcta acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag
     6541 ctcggttgtg atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg
     6601 ctggccacct tgttgggcct gtttgttgtc acatcctgtt agggctaagc tgcctgagag
     6661 catcagaacg accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt
     6721 gtccgatttc aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta
     6781 tcgtcgtgct atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac
     6841 gaacatatgg cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat
     6901 gtggctctac gtcttttctc acagcagggg gtatcatcca cctcgctggg tgagattgca
     6961 aaagcagctg gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg
     7021 ttcagtgaga tctgggaact gtcagaatcc aatattggtg aactagagct tgagtatcag
     7081 gcaaaattcc ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa
     7141 tccacggtga cagaagaacg acgtcgatta ttgatggaga ttatattcca caaatgcgaa
     7201 tttgtcggag aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat
     7261 gaccgtatag aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta
     7321 atgacgcgtc gggcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg
     7381 ctctttgccc cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt cgccatctta
     7441 ctggagatgt atctcctgtg ccccacgctt cgtaatcctg ccactaacga ataaccctga
     7501 atctgactcc aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg
     7561 tgatattctt gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat
     7621 catgactatg ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc
     7681 ttttgttttt gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga
     7741 tctgccgaca aaagcggacc tgcaggcgca acttgactca ctaaataaac aaaaagatct
     7801 ttctgctcag gacaaactgg agctgcagga tctgacagat acattagcca ccctcgataa
     7861 aatcgatcgc ataaaagaag agacagttca gctacggcaa aaagtcgctg aagcgccgga
     7921 aaaaatgcgc caggcgaccg cggcgttaac agcacttagc gatgtcgata acgacgaaga
     7981 aacgcgcaaa attctgagta cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc
     8041 gctggacgat ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc
     8101 gttacagacg cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca
     8161 acaaattcgc agtcgtctgg atgggactga tgtcggcgag actgccttac gtcccagcca
     8221 gaaagtgtta atgcaggctc agcaggcgtt gctgaatgcg gagattgacc agcagcgtaa
     8281 aagcctggaa gggaacaccg tcttgcagga taccttgcaa aagcaacgtg attacgtgac
     8341 ggcgaacagc gcccgtctgg agcaccagtt gcagctgttg caagaagcgg taaacagcaa
     8401 gcgcctgact ttaactgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg
     8461 tattcaggct aatccgctgg tgaaacagga actggaaatt aaccagcagt taagtcagcg
     8521 tctgattacc gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa
     8581 ctggctggag cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa
     8641 gggcagcctg ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga
     8701 tgaactggaa aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa
     8761 ccagcagcgt gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca
     8821 caccaacgaa gtcaacagcg aagttcacga tgcgttattg caagtggttg atatgcgtcg
     8881 cgaattgctg gatcaactca acaaacagtt gggtaaccag ctgatgatgg ccattaacct
     8941 gcaaatcaac cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca tcctgactca
     9001 gcaaatcttt tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc
     9061 gcaaagcctg aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg aaaaagcctg
     9121 gcctgccgtt tttatcgctt tcctcgccgg tttgccgctg ctgttgattg tcgggctgat
     9181 ccactggcgt ctgggctggc tgaaagcgta tcaacaaaaa ctggcttccg ctgtgggttc
     9241 cctgcgtaac gatagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgcgc
     9301 gctgccggtg tgcctgatta ttctcgcggt tggcttgatt ctgttgacca tgcagctcaa
     9361 catcagcgaa ctgctatggt cgttcagcaa aaaactggcg attttctggc tggtgtttgg
     9421 cctgtgctgg aaggtactgg agaagaacgg cgttgccgtg cgtcacttcg gcatgccgga
     9481 acagcagacc agccactggc gtcggcaaat tgtccgcatc agtctcgcat tgctgcctat
     9541 ccatttctgg tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg
     9601 gcaagcgatg attttcttca acctgctgct gattgccttc ctggtatggc caatgtgccg
     9661 cgaaagctgg cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtgctgtc
     9721 gattatcccg attgcattga tggtgctgac tgccacgggt tatttctaca ccacgctgcg
     9781 cctctccggg cgttggattg aaaccgttta tctggtgatc atctggaact tgctctacca
     9841 gacggtattg cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc
     9901 gcgtcggcag aatctggtga aagagggcgc agaaggtgcc gaaccgccgg aagaacccac
     9961 cattgcactg gaacaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc
    10021 gctgttcggt gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatat
    10081 cgacagcatt acgctctggc attacaacgg tactgaagct ggcgctgcgg tggtgaaaaa
    10141 cgtcaccatg ggcagtctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat
    10201 tcgcaacctg cctggtttgc tggaagtgct ggtgctctcg cgactgaata tgcgccaggg
    10261 cgcgtcgtat gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac
    10321 ggtgttcgga tcgctgggcg tctcttggga taaactccag tggctggcgg cagcattatc
    10381 cgtaggtctt ggttttggtt tacaagaaat tttcggtaac ttcgtctccg gtttgatcat
    10441 tctgttcgaa cgtccggtgc gtattggcga tacggtaacc attggtagct tctcggggac
    10501 ggtaagtaag atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat
    10561 catcccgaac aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacactac
    10621 tacgcgtctg gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa
    10681 agtgttactg aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt
    10741 cttctttacg gcgtttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga
    10801 actgcgtgac cgtagtcgta ctgtcgatga gctgaaccgt actatcgatc agctgtgccg
    10861 tgaaaacgac atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg
    10921 cgatgaggtg acggaagtaa aacgcgacta caaaggcgat gacccgacgc cagcggtagg
    10981 gtaataacca aagggggcga catttagttg ccccgagatt actgacaaag tgcgcgttgt
    11041 tcatgccgga tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatgta
    11101 ttgcaggagc ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccac
    11161 aatcttaggg gacatttagc ggccccattt atttctcact tttccgcctc atcatcgcgc
    11221 gttaatttct tttcataatc gcgttttaca atatccagcg cgcgcagcac ggtgcgggct
    11281 gggatctgat tttcttccag cagcacaatc aaatcgacag ccagtttgac atcgtcaggg
    11341 gcattttcca gtgacatact ctctccattg ctagcgggtt aagcgcgcta gcctgttttc
    11401 gattttttcc agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg
    11461 atgcagcgtt tgctgttcca cgagatcggt cacccgcgcc aggcgggctc tgcgttcggc
    11521 aaccatctca cgtagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg
    11581 ggcaattttc ggtggcgcac tgtcccactc gcgcaatgac caggcggagg cttcacgcgc
    11641 gatggcttcc agttgtgccg ccagatgttc cgccagccag gccacttgcg gcagctgttg
    11701 ctgctcaact gcatgacgaa gcgcagccag attatcgcca gcctcatcga gacaagcctg
    11761 cagtgttgtc gtacgggttt gaaaaaggtg tctgtcaaaa cgggcactta gcgtggcaaa
    11821 ttgcgctacc ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag
    11881 cagcagggcg gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag
    11941 ccactctgta agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg
    12001 ctggcggtag tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt
    12061 atcctgctgg cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg
    12121 taccgctcat ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc
    12181 ggcgtcaaac cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc
    12241 gtccagatgc catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttctat
    12301 atgtggcgaa ttccggtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca
    12361 gttgcaatta ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt
    12421 caaaggttaa acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa
    12481 caagcaggca tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca
    12541 tcaaaagcat tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac
    12601 tggaagaccc gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg
    12661 cgggcattac caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc gctccggtag
    12721 ctctgggtct gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa
    12781 ccatcagtga aacttacgac ctggaatacg gcaccgatca gctggagatc cacgttgatg
    12841 ccatcaaacc gggcgacaaa gttctggtgg tggacgatct gctggcaacc ggcggcacta
    12901 tcgaagcgac cgttaaactg atccgtcgtc tgggtggtga agtggctgac gctgcgttca
    12961 ttatcaacct gttcgatctc ggcggcgaac agcgtctcga aaaacagggc attaccagct
    13021 acagccttgt cccgttcccg ggccattaat tatcgccagt cttgtgctgc ccacgctacg
    13081 gacagcacaa gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc
    13141 ttcgtgaatc caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg
    13201 aaaatggcgc ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact
    13261 ggcgaacggc ttgtcgttag ggcgtattca tcatgcttat cttttttccg gtacccgtgg
    13321 cgtcggaaaa acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat
    13381 taccgcgacg ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt
    13441 cgatctgatt gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc gcgacctgct
    13501 ggataacgtc cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga tcgacgaagt
    13561 gcatatgctg tcgcgccaca gctttaacgc actgttaaaa acccttgaag agccgccgga
    13621 gcacgttaag tttctgctgg cgacgaccga tccacagaaa ttgccggtga cgattttgtc
    13681 gcgctgtttg caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcaacttga
    13741 gcacatcctc aacgaagaac atatcgctca cgagccgcgg gcgttgcaat tgctggcgcg
    13801 cgccgctgaa ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagcgg
    13861 tgacggccag gtttcaaccc aggcagtcag tgcgatgctg ggtacgcttg atgacgatca
    13921 ggcgctgtcg ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat
    13981 taatgaagcc gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggcct
    14041 gttgcatcgt attgcgatgg tacaactttc gcctgctgca cttggcaacg acatggccgc
    14101 cattgagctg cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta
    14161 ctatcagacg ctgttgattg gtcgcaaaga attaccgtat gcgccggacc gccgcatggg
    14221 cgttgagatg acgctgctgc gcgcgctggc gttccatcca cgtatgcctc tgcctgagcc
    14281 agaagtgccg cgccagtcct ttgcacccgt cgcgccaacg gcagtaatga cgccaaccca
    14341 ggtgccgccg caaccgcaat cagcgccgca gcaggcaccg actgtaccgc tcccggaaac
    14401 caccagccag gtgctggcgg cgcgccagca gttgcagcgc gtgcagggag caaccaaagc
    14461 aaaaaagagt gaaccggcag ccgctacccg cgcgcgtccg gtgccatcgg cgctggaaaa
    14521 agcgccagcc aaaaaagaag cgtatcgctg gaaggcgacc actccggtga tgcagcaaaa
    14581 agaagtggtc gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa aaacgccgga
    14641 actggcggcg aagctagcgg cagaagccat tgagcgcgac ccgtgggcgg cacgggtgag
    14701 tcaactttcg ctaccaaaac tggtcgaaca ggtggcctta aatgcctgga aagaggagag
    14761 cgacaacgca gtatgtctgc atttgcgctc ctctcagcgg catttgaaca accgcggtgc
    14821 acagcaaaaa ctggctgaag cgttgagcac gttaaaaggt tcaacggttg aactgactat
    14881 cgttgaagat gataatcccg cggtgcgtac gccgctggaa tggcgtcagg cgatatacga
    14941 agaaaaactt gcgcaggcgc gcgagtccat tattgcggat aataatattc agaccctgcg
    15001 tcggttcttc gatgcggagc tggatgaaga aagtatccgc cccatttgat cgtaagcaca
    15061 gcttacgttc gtcatcctta acgtgattga gagagaaacc tatgtttggt aaaggcggtc
    15121 tgggtaacct gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa atgcaggaag
    15181 agatcgcgca gctggaagtc accggcgaat ctggcgcagg tctggtaaaa gtgaccatca
    15241 acggtgcaca caactgccgt cgcgtagaga tcgacccgag cctgctggaa gacgacaaag
    15301 agatgctgga agacctggtg gctgcagcat tcaacgacgc agcacgtcgt attgaagaaa
    15361 cgcagaaaga aaaaatggcc tctgtatcct ccggaatgca gctgccgcct ggctttaaga
    15421 tgccgttctg atgcaaacca gcccgctgtt aacacagctt atggaagcac tgcgctgtct
    15481 gtcgggcgtt ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc agcgcgatcg
    15541 tagcggcggg atgcgtctgg cgcaggcgct cacccgggcg atgtcggaaa tcggccactg
    15601 cgccgattgc cgcactttca ccgaacagga agtctgtaac atctgttcga atccgcgtcg
    15661 tcaggaaaac ggtcaaatct gtgtggtgga gagtccggcg gacatctacg ccattgagca
    15721 gacggggcag ttttctggtc gttattttgt gttgatgggg catctgtcac cgctggacgg
    15781 catcggtccg gatgatatcg ggcttgatcg tctggaacag cgtctggcag aggaaaaaat
    15841 cactgaagtg atcctcgcca ccaatcccac ggttgaaggt gaagctaccg ctaactacat
    15901 tgccgagctt tgcgcgcaat atgacgtgga agctagccga atcgctcatg gcgtaccggt
    15961 tggcggcgaa ctggaaatgg tcgatggcac cacgctgtca cactcccttg ccgggcgtca
    16021 taagattcgt ttttaagcaa acgagagcag gatcacctgc tctcgcttga aattattctc
    16081 ccttgtcccc atctctccct catcctgttt ttaaccttaa aatggcatta ttgaggtaga
    16141 cctacatgaa aggacaagaa actcgtggtt ttcagtcaga agtgaaacag cttctgcacc
    16201 tgatgatcca ttctctctat tccaataaag aaatcttcct gcgtgagctt atctctaacg
    16261 cctccgatgc ggcggacaag ctgcgtttcc gtgcgctatc taacccggac ctgtacgaag
    16321 gtgatggcga actgcgcgtt cgtgtctctt tcgataaaga caagcgcacg ctgaccatct
    16381 ccgataacgg cgtggggatg acccgcgacg aagtgattga ccatctgggg actatcgcta
    16441 aatccggtac caaatcattc ctcgaatccc tgggttctga ccaggcgaaa gacagccagc
    16501 tgatcggtca gtttggtgtt ggtttctact ctgcgtttat cgtggccgac aaagtgactg
    16561 tgcgtactcg cgcggcaggc gaaaaaccag aaaacggcgt cttctgggaa tcggctggcg
    16621 aaggtgaata taccgttgcc gacatcacca aagaagatcg tggtactgaa atcaccctgc
    16681 atctgcgtga aggcgaagac gagttcctcg atgactggcg cgtgcgttcc atcatcagca
    16741 aatactccga ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag aaagacggcg
    16801 aaaccgttat ctcctgggag aaaatcaaca aagcgcaggc gctgtggact cgtaacaagt
    16861 cggaaatcac cgatgaagag tacaaagagt tctacaaaca catcgcccac gactttaatg
    16921 atccgctgac ctggagccac aaccgcgttg aaggtaagca ggagtacacc agcctgctgt
    16981 acatcccgtc ccaggctccg tgggatatgt ggaaccgtga tcataaacac ggcctgaaac
    17041 tgtacgtcca gcgtgtgttc atcatggacg acgcagaaca gttcatgccg aactatctgc
    17101 gcttcgtgcg tggtctgatt gactccagcg atctgccgct gaacgtttcc cgtgaaatcc
    17161 tccaggacag cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt gtgctgcaaa
    17221 tgctggaaaa actggcgaaa gacgacgcgg aaaaatacca gaccttctgg caacagtttg
    17281 gcctggtact gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg atcgccaaac
    17341 tgctgcgttt tgcttctacc cataccgatt cttctgcgca gaccgtatct ctggaagact
    17401 acgtttcccg catgaaagaa gggcaggaga aaatctacta catcaccgca gacagctatg
    17461 cggcagcgaa gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc gaagttctgc
    17521 tgctttccga ccgcatcgat gagtggatga tgaactatct gactgagttc gacggtaaac
    17581 cgttccagtc ggtgtctaaa gttgacgagt cgctggaaaa actggctgac gaagttgatg
    17641 agagcgcgaa agaagcggag aaagcactga ctccgttcat cgaccgtgtg aaagccttgc
    17701 tcggcgagcg cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg ccagcgatcg
    17761 tttcgaccga cgcggacgaa atgagcactc agatggcgaa actgttcgct gcggcgggcc
    17821 agaaagtgcc agaagtgaaa tacatcttcg aactgaaccc ggatcacgta ctggtgaaac
    17881 gtgcggcaga tactgaagat gaagcgaagt tcagcgagtg ggtagaactg ctgctggatc
    17941 aggcgctgct ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt cgtcgtatga
    18001 accagctgct ggtttcctga tgtcatgccg gatgaccttt gtgtcatccg gcatttttct
    18061 tttcatcatc tgcactttcc gcaaattatc tcgccattaa ccgtttcagc ctcaggtgcc
    18121 tttcttgagg caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aattcgacac
    18181 attttaaggg gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag
    18241 ggactcaggc tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata
    18301 tgctgcgtgc tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa gacattatgg
    18361 atgctggcaa actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc
    18421 aggaagactg ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag
    18481 acgcgatgaa agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg
    18541 aactgattgt tgatcgtatc gtaggccgcc gcgttcatgc gccgtctggt cgtgtttatc
    18601 acgttaaatt caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagaactga
    18661 ctacccgtaa agacgatcag gaagaaaccg tacgtaaacg tctggttgaa taccatcaga
    18721 tgactgcacc gctgatcggc tactactcca aagaagcgga agcgggtaac accaaatacg
    18781 cgaaagttga cggcaccaag ccggttgctg aagttcgcgc tgatctggaa aaaatcctcg
    18841 gctaatcaag ccgcagcaga tctgctttcg ggcaggtctg ctcatccacc tcaggcaata
    18901 cctctcacag caattagttc ttcttcctca cttttccgct acaattatca acaagttgaa
    18961 tcgataagag gcggtaatgc gtcagactaa aaccggtatc ctgctggcaa acctgggtac
    19021 gcccgatgcc cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag
    19081 acgcgtggtt gatacctcac ggttgttatg gtggccattg ctgcgcggcg ttattttgcc
    19141 gctgcgctcg ccgcgtgtgg cgaagctgta tgcctctgtc tggatggaag gtggctcgcc
    19201 gctgatggtt tacagccgcc agcaacagca ggcgctggca caacgtttac cggagacgcc
    19261 cgtagcgctg ggaatgagct acggctcgcc atcactggaa agcgccgtcg atgaactcct
    19321 ggcagagcat gtagatcata ttgtggtgct gccgctttat ccgcaattct cctgttctac
    19381 ggtcggtgcg gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg
    19441 gatatcgttt attcgtgatt acgccgataa ccacgattac attaatgcac tggcgaacag
    19501 cgtacgcgct tcttttgcca aacatggcga accggatctg ctgctgctct cttatcatgg
    19561 cattccccag cgttatgcag atgaaggcga tgattacccg caacgttgcc gcacaacgac
    19621 tcgcgaactg gcttccgcac tggggatggc accggaaaaa gtgatgatga cctttcagtc
    19681 gcgctttggc cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg
    19741 agaaaaaggc gtaggtcata tacaggtgat gtgcccgggc tttgctgcgg attgtctgga
    19801 gacgctggaa gagattgccg agcaaaaccg tgaggtcttc ctcggtgccg gcgggaaaaa
    19861 atatgaatat attccagcgc ttaatgccac gccagaatat atcgaaatga tggctaatct
    19921 tgttgccgcg tatcgctaaa gctgagcggt aaagaactga gcgccgtcgc gaagagcctc
    19981 gtcggcggtt ttcatcattc gtgaataatg caaaaaggca tgcagcgtac ctgggtagag
    20041 tttgaactca cagggctgct gatgcgccgc taacgtctgg taaagcagac ggctgtcatc
    20101 cagcagcgga tcgaactccg ccccggcaat aaaacagggc ggaacttcgc gagtgagatc
    20161 attattaaac agacagtaat acggtgactc gcggtccgcg tcgttgctta aatacgcctc
    20221 ttcatacatc tgcaaatcct gttgcgttaa gccatcccag acaccgccca acagacgacg
    20281 agtcacggaa tcccgtaatc cgtaaagccc ataccacagc aaaacacccg caattttacc
    20341 gcaatcgatc tgtttatcac gcaaccacaa cgcactggcg agcgccagca tggcacctgc
    20401 ggaatcaccg gcaaagccaa tgcgggacat attgatttga taatcctccg cctgctggtg
    20461 gaaataacaa caagcagcca caatttcctc tatcgcttgc ggaaaacgcg cttcaggtga
    20521 aagggggtaa tcaataccaa tcaccgtaca ttggctgtag cttgccagca ggcgcatgat
    20581 gcgatcgtgg gtatcgagat tgccgagaat aaaaccgcct ccatgcaaat aaaatagcgt
    20641 cgccgggcta tctggctgcg gacaaaagag acgtgtttcc acctgcccat attttgttgg
    20701 aaccatgtaa gctctggttg ccatttctgg agcgcccgca ttccagaatc ggcgctcaag
    20761 cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg ggccaggacg gtaaatccgg
    20821 ctgaagagta ttcacaacgg tcttcatttc agcagaaata aggtccagaa caggtagttt
    20881 gttttccggc ttcatacaaa aactcctttc aaattacgtc attgtaagga accactgcca
    20941 tgaaaatgcg atcccgcctg ctgatattga aactggctgc gtctcgcgcg ctcccgtcag
    21001 attgtgttaa cattcgccgc tcagttaacc acccgtaaaa acaaccatga aatttcccgg
    21061 taaacgtaaa tccaaacatt acttccctgt aaatgcacgc gatccgctgc ttcagcaatt
    21121 ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt attgaccaga cgctggtcga
    21181 tattgaagcg aaagtggatg atgaattcat tgagcgttat ggattaagcg ccgggcattc
    21241 actggtgatt gaggatgacg tagccgaagc gctttatcag gagctaaaac agaaaaacct
    21301 gattacccat cagtttgcgg gtggcaccat tggtaacacc atgcacaact actcggtgct
    21361 cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa ttggcagtta
    21421 tgcctatcgt tacctgtgta acacttccag ccgtaccgat cttaactatc tacaaggcgt
    21481 ggatggcccg attggtcgtt gctttacgct gattggcgag tccggggaac gtacctttgc
    21541 tatcagtcca ggccacatga accagctgcg ggctgaaagc attccggaag atgtgattgc
    21601 cggagcctcg gcactggttc tcacctctta tctggtgcgt tgcaaaccgg gtgaacccat
    21661 gccggaagca accatgaaag ccattgagta cgcgaagaaa tataacgtac cggtggtgct
    21721 gacgctgggc accaagtttg tcattgccga gaatccgcag tggtggcagc aattcctcaa
    21781 agatcacgtc tctatccttg cgatgaacga agatgaagcc gaagcgttga ccggagaaag
    21841 cgatccgttg ttggcatctg acaaggcgct agactgggta gatctggtgc tgtgcaccgc
    21901 cgggccaatc ggcttgtata tggcgggctt taccgaagac gaagcgaaac gtaaaaccca
    21961 gcatccgctg ctgccgggcg ctatagcgga attcaaccag tatgagttta gccgcgccat
    22021 gcgccacaag gattgccaga atccgctgcg tgtatattcg cacattgcgc cgtacatggg
    22081 cgggccggaa aaaatcatga acactaatgg agcgggggat ggcgcattgg cagcgttgct
    22141 gcatgacatt accgccaaca gctaccatcg tagcaacgta ccaaactcca gcaaacataa
    22201 attcacctgg ttaacttatt catcgttagc gcaggtgtgt aaatatgcta accgtgtgag
    22261 ctatcaggta ctgaaccagc attcacctcg tttaacgcgc ggcttgccgg agcgtgaaga
    22321 cagcctggaa gagtcttact gggatcgtta agttatcgtc ggttcgtagg ccagataagg
    22381 cgttcacgcc gcatctggca tttggctctc gatgcctgat gcgacgctgg cgcgtcttat
    22441 catgcctaca tatttttcat attttacatc cggcaaccac cgtttacccc gtcaccacct
    22501 cacccgccgg tggcgtttcc agcagttcca gcatggtacg ggcgatttca cgctcgccca
    22561 tcactacctg attcgcacca cgttcggtga tatacgccac ttcatcgtca taatgggcgc
    22621 gggcaataat ctcaatatcc ggatttttcg cgcgggcaga tgccacaatc tcacccgctt
    22681 cataaccgtt gggaatcgtc aggatcagcc attttgcaca ttccagatgc gccagttgca
    22741 taatttcttc gttcgccgca ttgcccaata ctgcgcggac cccgcgctct cgcagctcat
    22801 caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg agcaatttct
    22861 cccccagcag gctgcctaca cgaccgtaac ccaccagtag cgcatggttg caaatatcca
    22921 ctgggatctg cttctcttct tcgattgcct cttccagcgt ctgctcttcc agcgtttcgg
    22981 tcttcgccag atatttctcc agtagtgcga acagtaccgg gttgagcata atcgacagga
    23041 tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag taaattcaat gccattccca
    23101 gtcccgccag gataaacgcg aactcaccaa tctgcgccag gctggcggcg atggttaatg
    23161 ccgtacgttg ggagtgacca aacagtcgca ccaggaaaaa tgcggctaac gacttaccaa
    23221 acagaataat cgccagcgtc gccagcactg ccagcggttg ctgaatcaga attaacggat
    23281 caaacaacat cccgacggag acaaaaaaca gcaccgcaaa cgcgtcgcgc aatggcagcg
    23341 tatcgtgggc ggcacggtga ctcagttcag actcgttcag taccatcccg gcaaagaacg
    23401 caccgagtgc aaaggagaca tcaaacagct ctaccgcacc aaaggcaatc cctaacgcca
    23461 gcgccagcac cgacagggta aacagctcgc gagaaccggt tgccgcgctg cgtgccataa
    23521 tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg atcactttgc
    23581 cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc tgttccatca
    23641 ttcctgcaac tgcgggcagc aacaccagcg tcagaaccat taccaggtct tccacaatca
    23701 accaaccgat ggcgatttgc ccacgctgac tgtcaattaa ttgccgttct tcaagtgcgc
    23761 gcagtaacac cacggtactg gcggtggaaa gacataaacc gaacacgata ccggtcatta
    23821 acgaccagcc cagcacggca gagagcgcca tacccagcag cgtcgccacg gctatctggg
    23881 cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc gaaaagtgca
    23941 aaccgacgcc aaacatcaac agaatgacgc ccagttcagc cagttccggc gcaagcttgg
    24001 tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac agatatccca
    24061 ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg agcacaaggc
    24121 cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct tttcctggtg
    24181 gttattgtcc atttttggcc gggaaaacca aaattacagg taatagttta tgacaatttc
    24241 attgatgatg ttcatgaata attattgaat tttgcagaaa aatggaatta gctgcaaaaa
    24301 aagcacggat cggaaaacag aaggcggttt aacagaggaa aggcttatgg cgactgtaaa
    24361 acgatgcagc caaagtttgc ctttggctgc aatgaaatca gtctttatgc cggttatcag
    24421 gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc acagatttta tagactaact
    24481 cgatgctggt gtgatcggcg ataagcccca gaactgccgc tcccagacct cccatgccaa
    24541 aagcaaaacc gaaaaagagt ccagaaacca taccgatacg tcccggaagt agctcctgag
    24601 cgtagaccag aatggcagag aatgccgaag cgaggataaa tccaataatc accgttaaaa
    24661 cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt aaacggcgca acgccgagga
    24721 tagagcccca aatcacatat ttccgtccaa ttttatcccc tacaggcccg ccgatcaccg
    24781 tacctgccgc aacggcaaac aggaaggcaa acagatgaag ctgggcattc tggatagata
    24841 atcc
//
LOCUS       DAJVOY010000045        24814 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVOY010000045 DAJVOY010000000
VERSION     DAJVOY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.045"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(83..2302)
                     /locus_tag="OQL90_001632"
     CDS             complement(83..2302)
                     /locus_tag="OQL90_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1368451.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(2413..3825)
                     /gene="ftsP"
                     /locus_tag="OQL90_001633"
     CDS             complement(2413..3825)
                     /gene="ftsP"
                     /locus_tag="OQL90_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1368452.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3900..4637)
                     /gene="plsC"
                     /locus_tag="OQL90_001634"
                     /gene_synonym="parF"
     CDS             complement(3900..4637)
                     /gene="plsC"
                     /locus_tag="OQL90_001634"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1368453.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4870..7128)
                     /gene="parC"
                     /locus_tag="OQL90_001635"
     CDS             complement(4870..7128)
                     /gene="parC"
                     /locus_tag="OQL90_001635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1368454.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSLMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7674..8156)
                     /locus_tag="OQL90_001636"
     CDS             complement(7674..8156)
                     /locus_tag="OQL90_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCS1368455.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8209..8601)
                     /gene="ygiW"
                     /locus_tag="OQL90_001637"
     CDS             complement(8209..8601)
                     /gene="ygiW"
                     /locus_tag="OQL90_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1368456.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8753..9412
                     /gene="qseB"
                     /locus_tag="OQL90_001638"
     CDS             8753..9412
                     /gene="qseB"
                     /locus_tag="OQL90_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1368457.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9409..10758
                     /gene="qseC"
                     /locus_tag="OQL90_001639"
     CDS             9409..10758
                     /gene="qseC"
                     /locus_tag="OQL90_001639"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1368458.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10868..11449
                     /gene="mdaB"
                     /locus_tag="OQL90_001640"
     CDS             10868..11449
                     /gene="mdaB"
                     /locus_tag="OQL90_001640"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1368459.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11480..11794
                     /locus_tag="OQL90_001641"
     CDS             11480..11794
                     /locus_tag="OQL90_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1368460.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11842..13734)
                     /gene="parE"
                     /locus_tag="OQL90_001642"
     CDS             complement(11842..13734)
                     /gene="parE"
                     /locus_tag="OQL90_001642"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1368461.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13763..14344)
                     /gene="yqiA"
                     /locus_tag="OQL90_001643"
     CDS             complement(13763..14344)
                     /gene="yqiA"
                     /locus_tag="OQL90_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1368462.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14344..15171)
                     /gene="cpdA"
                     /locus_tag="OQL90_001644"
     CDS             complement(14344..15171)
                     /gene="cpdA"
                     /locus_tag="OQL90_001644"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1368463.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15196..15618)
                     /locus_tag="OQL90_001645"
     CDS             complement(15196..15618)
                     /locus_tag="OQL90_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1368464.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15619..16248)
                     /gene="nudF"
                     /locus_tag="OQL90_001646"
     CDS             complement(15619..16248)
                     /gene="nudF"
                     /locus_tag="OQL90_001646"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1368465.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16453..17934
                     /gene="tolC"
                     /locus_tag="OQL90_001647"
     CDS             16453..17934
                     /gene="tolC"
                     /locus_tag="OQL90_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1368466.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            18082..18753
                     /locus_tag="OQL90_001648"
     CDS             18082..18753
                     /locus_tag="OQL90_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1368467.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18759..19919
                     /locus_tag="OQL90_001649"
     CDS             18759..19919
                     /locus_tag="OQL90_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1368468.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19957..20745)
                     /gene="ygiD"
                     /locus_tag="OQL90_001650"
     CDS             complement(19957..20745)
                     /gene="ygiD"
                     /locus_tag="OQL90_001650"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1368469.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20888..21649
                     /gene="zupT"
                     /locus_tag="OQL90_001651"
     CDS             20888..21649
                     /gene="zupT"
                     /locus_tag="OQL90_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1368470.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21707..21877)
                     /locus_tag="OQL90_001652"
     CDS             complement(21707..21877)
                     /locus_tag="OQL90_001652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368471.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(22137..22790)
                     /gene="ribB"
                     /locus_tag="OQL90_001653"
     CDS             complement(22137..22790)
                     /gene="ribB"
                     /locus_tag="OQL90_001653"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1368472.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22894..23042)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23164..23454
                     /gene="ubiK"
                     /locus_tag="OQL90_001654"
     CDS             23164..23454
                     /gene="ubiK"
                     /locus_tag="OQL90_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS1368473.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            23738..24289
                     /locus_tag="OQL90_001655"
     CDS             23738..24289
                     /locus_tag="OQL90_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCS1368474.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            24349..>24814
                     /locus_tag="OQL90_001656"
     CDS             24349..>24814
                     /locus_tag="OQL90_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1368475.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNAIINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDA"
BASE COUNT         6190 a         6577 c         6166 g         5881 t
ORIGIN      
        1 ttcggcatat aaataaatgc ctaatgagat agattctgat gtaatatccc cggtagcaat
       61 tctgctaccg ggaacagcga atttaacgtc caaccgccgc tttaggccgt ttcttcgcac
      121 cagcattcac cggacgagat tgcgtagacg acgctttttt tgccgtagca ggcgtctgac
      181 gctgggtcgc catcggcgta tgtttcgtca acgccggacg ggtattgcgg ttctggcgac
      241 gagcttcacg catctcttca atggttggcg caggcactaa gcaatcgcga cggctaccaa
      301 tcagatgctt tttacccatc gcttccagcg cctggcggat taacggccag tttgccggat
      361 cgtggtaacg caacaacgct ttatgcaaac gacgctgctt gtcgcccttc ggtacgaaga
      421 cgtcttcact cttataacca atcttcgcca gcggtttttt cccggtgtaa tacatggtgg
      481 ttgagttcgc cagcggcgat gggtagaagt tctgtacctg gtcgagacgg aaacgatgct
      541 ttttcagcca cagcgccaga ttcaccatat cttcatcacg cgtaccgggg tgcgcggaga
      601 tgaaataggg gatcagatac tgctctttac ctgcctgttt tgagtaagta tcgaacagct
      661 ctttaaagcg gtcatagctg cccatgcccg gcttcatcat cttcgataac ggcccttctt
      721 cggtatgttc cggggcaatc ttcagataac cgccgacgtg atgggtcgcc agttctttga
      781 tatagcgcgg atcttccacg gcgatgtcat aacgcacacc agaggcaatc aggatctttt
      841 taatgccttt cagatcacgc gcacggcgat agaggttgat cgttggttcg tggttcgtgt
      901 ccatgtgcgg acaaatatcc ggataaacgc acgacaaacg gcggcaggtt tgctcagcgc
      961 gtggcgattt gcagcgcaac atatacatgt tggcagttgg cccaccgaga tcggaaatta
     1021 cgcccgtaaa acctggaacg gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt
     1081 cggaacggct ctgaataatg cgcccttcgt gctcggtgat agaacagaaa gagcagccgc
     1141 caaagcagcc acgcataatg ttgaccgaaa aacggatcat ttcgtaagcc ggaatacgga
     1201 cattgccata ggccggatgt agcacgcgct tgtatggcag cgcaaaaacg ctgtccatct
     1261 cttcggtaga aagcggaata gcaggcgggt tgatccacac atagcggtcg ccgtgttttt
     1321 gcatcaatgc gcgggcacag cctgggttgg tttcgtggtg cagaatacgc gaagcatggg
     1381 cgtacagcac tttatcgccc ttcactttct cgaaagaagg cagcaacacg taggtttttt
     1441 cccacggttt cgggcgcggt ggctgcacgg ttacggcttt ggcttcctgc tttttcggtg
     1501 ccaccggttt gttatccgcg cacggcaaat cttcaccata cggatgcggg attgggtcga
     1561 tttttccagg ggtatcaaga cgggtggaat ccacgccgct ccagccaggc agggcctctt
     1621 tcacgataat cgcggtatta cgcacatcgc ggatttcact aatcggctcg cccatcgcca
     1681 gacggtgcgc cacctccacc agcggacgct caccgttacc aaacatcagc atgtcggctt
     1741 tcgaatccac cagcacggaa cggcgcacgg tatcggacca gtaatcataa tgtgcggtac
     1801 gacgcagact agcctcaata ccgccgagga tcaccggtac atctttccac gcctctttac
     1861 aacgctgggt ataaaccagt gtggcgcgat ccgggcgctt acccgcgacg ttatccggcg
     1921 tgtaggcatc gtcatgacgt aaacggcgat cggcggtata acggttgatc atcgaatcca
     1981 tgttgccagc agtaacaccg aaaaacagat tcggtttacc cagacgcata aagtcgtctt
     2041 tgctgctcca gtccggctgg gcgatgatcc cgacgcgaaa gccctgcgct tccagcatac
     2101 gaccgcaaat cgccatcccg aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa
     2161 tgatgtcgca gctatcccag ccaagttgat ccatctcttc actagacatc ggcaaaaacg
     2221 gtgccggtcc aaaacaggcg gcccagtact gcggccagga gaacaggtcg cgatccggtt
     2281 ggatcaggga gatagagctc attttgcttc cagaaatgat aaaaaaataa tcaaaggccg
     2341 gggattataa gccggaacga aagagaaatc gaaaagtatt ccatactcgc cctcctcggg
     2401 cgagtatgaa gattacggta ccggattgac taacagttgc ccaatcgagc cacggtccgc
     2461 catttccagc gtctgactgt tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa
     2521 ataaacaagc agctccactt gtccgtcaac ccaaacggta tctttccagc ctctgtcttc
     2581 cgggaacggc atcgcaccat tcacgttacg gatctggaac atcacgcctt caatatggaa
     2641 cgcttgcggc tcgtccgcgc gtaccgtcca gcgttcccac gttccttgct gcgcggtgac
     2701 atcaatacgg ttgacgtccc acagttgtcc attaataccc gggtcatcac ccagactgat
     2761 atcgcgactg cgaatcggcg aaccggccat gatttcagtt ggcagcaagc gcatcggaag
     2821 actgtctgtg accagcggca gaaggccggt tgggcgcagc gttagcacca gggtagaaac
     2881 cagaatgctg gatggctcaa agaagccacg aatacgatca acaatgctcg ccgcttcgcc
     2941 acaggtgatc gacacttcat caccgttgct catatccacc agaatctcgc ggcgctcgcc
     3001 cggtgccaac gaaagttgct tcaccgatac aggagcaggc aggaatccct gatcgccaga
     3061 aatcacatgt aacgggcgac tatcgctcat ctgtagttga tagcgacgag agttcgacgc
     3121 gttcagcagt cgcaagcgca cccagccacg cgagacttca acgtacgggc tttgtacacc
     3181 gttaaccagc agcgtatcac caacaaagcc gccgcttccc ggttcgttgt attctggcgt
     3241 accaaagtta tccagccgtt tatcctggat aatgaccgga aaatcatcca caccataatg
     3301 gttggggata ggcagcgact tgctgacttc atcttccacc agccacattc cggcaaggcc
     3361 gttatagacc tgctgagccg tgcggttggg agtattggcg tgataccaca gagtagctgc
     3421 gttctggcga atgggcagta cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg
     3481 accgcccatc agcgggcctg gtacctgtag cccggccacc gtcattgaga cattttctgt
     3541 caggcggttg ctgtaaataa gcttaacatc gtcgcccttc cagacgcgga tagtcggccc
     3601 caggtaacga ccattgattc cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg
     3661 cgcacgttgt acagtcataa acagcggttg cccacggcga gattcaagta gcggcggaac
     3721 gggtagcggt tgctgttgcc cggctgcgct ggccttcagg ggaacagcgc cggcacaaag
     3781 tgcaatcccc gatgcctgaa tgaactgacg ccgactgagt gacatatttg ctccatgtaa
     3841 aactggctaa agtaataccg tgcaggaaag tgttccccgc aaaaatacag attgccgact
     3901 taaacttttc cggcggcttc gcgttctgcg acttctttat cgagctcggc gattttttgt
     3961 tccattatcg aacgacaatg ggcagccagc tcacgaacct gatctttgcc atactgactg
     4021 acgtcaattg gcggcagcat ttcgacaatc accagaccgt tgtgcagtcg attaagatta
     4081 atcttattcg aagttgtaga gacgcacacg ggaataatcg ggacgcccgc cgcaattgcc
     4141 gcgtgaaatg ctccagtctt gaacggtagc aggccgcgac cacggctgcg ggttccttcc
     4201 gggaacatcc agatggaaat acggcgtttt ttgaaatgat tcacgacttc cgcaatggtg
     4261 ccgtgagctt tagtgcgatt gtttctgtcg atcaataagt tgccggttaa ccagtacaac
     4321 tgcccgaaga aggggatcca cagcaagctc tttttaccta ccgtcaccgt cggcggttgc
     4381 acaatgttcg atgctgtcac catgtcatag ttgttctggt ggttagcgat atagatagca
     4441 ttgccgtagc tttcagcatc tgcaggtttg cggcactcaa ctttcaggcc gaacagcggc
     4501 gcaagacggc caaacatatg cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa
     4561 aggcagtaaa tggagccgaa tacacagact aagatgctgt aaatcacggt aataataaga
     4621 cgaaagatat atagcataac aacctctgaa ggtctgagag actggcattg tacgtcacct
     4681 gaaatcaggt tagggcattg ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa
     4741 cgcatgaata aacaacggtt tcaagggaat ttttatcgtt tccgggggtt gaatacgctg
     4801 ccggatgacg acttaacgtt tcatccggcg ttccttgcaa gcgggaggaa acaggccctc
     4861 cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt cgatctcaac
     4921 acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt cgccagtgac
     4981 tttctgtaac tcttccgggc gcagtttaat tttgcgtttc ccaacatgaa tggtcagcgt
     5041 gctttgcggc ggcagaacgt acaattgcgc cagaccatct tctccacgcg cggcttctgc
     5101 cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca gatcgcttac
     5161 cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg aagcatcttc
     5221 aatcaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag ccttacctgc
     5281 acggttacgc gccaccagat cgttaaaggt gcagacgaaa ccgtaacccg catcggaagc
     5341 catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg ccccaggcgg
     5401 caacgttaat ttgccggtga gtggttcgcc ctgaccacgc gccgacggca gcgtaatcgg
     5461 gtcgatggca tagctacgac cggtggaatc aacaaacact accggttggt tgctcttacc
     5521 tttcaccgcc gctttgaagc tatcacccgc tttataattc aggcccggcg cgtcgatatc
     5581 atggccttta gcgctgcgta cccagcccat cagcgacagc acaatggtga caggttcaga
     5641 cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcctgca acggcgaacg
     5701 acgatcgtca ccgtaggctt gcgcgtctgc ctgcagttct ttcttcagca ggttattcat
     5761 tttacgctcg gaaaccaaaa tgccctgcaa ctggtcgcgc tctttttcca gctcactctg
     5821 ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca gttcgaggat
     5881 cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt tcggttcatc
     5941 ttcgttacga atgatctcaa tcacttcgtc gatgttgaga aacgccacca gcaaaccttc
     6001 caggatatgc aggcgcttga ggactttctc cagacgatag ttcagtcggc ggcgcacggt
     6061 atcgcgacgg aacaccagcc attcggagag gatttccagc aggtttttca ccgccggacg
     6121 accatccaga ccgatcatat tgaggttaat gcgatagctc ttttccagat cggtggtagc
     6181 gaagaggtgg ttcatcacct gatccatatc cacgcggttg gaacgcggca caatcaccag
     6241 acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg gcagcttttt
     6301 gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct gatgcggcaa
     6361 tgcgctgata accaccgcgc catcttcttt cttccacacc gcgcgcatac gcactgaacc
     6421 acgtccgttc tcgtagattt tacggatctc ggcgcgcgaa gtgataattt ccgcttcagt
     6481 cggataatcc ggcccctgca cgatatccag cagctgatcg agcgtggttt tcggctggtc
     6541 gattaatgcg attgccgcct gagctacttc acgcaggtta tgcggtggaa tatcggtcgc
     6601 catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac gggcaggcag
     6661 cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg tcccctgccc
     6721 cagctcgctc aatagcagtt cggaatattt cgacaaccgg gattcggtgt aacgcattgc
     6781 cgcgaacgat ttcggatcgt ccggcgcgcc ccagttcccc tgaccatcaa ccagcggata
     6841 acggtaagag aacggctgcg ccatcaggac catcgcttca taacaggcac tatcgccgtg
     6901 cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa atttggcgct
     6961 ggcattcagg cccagttcag acatcgcata cacaatgcgg cgctgaacag gtttaagacc
     7021 atcgccaata aacggcaacg cacggtccat gatcacgtac atggagtagt ttaagtaggc
     7081 gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta attctgattc
     7141 ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac attatctgcc
     7201 gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg acggcgataa
     7261 aggcatcaat gtgccttgat atacaactct ttgctgtaga cttgatcggc atagagggca
     7321 gcacaaggtt gcgcccatgc cacaccactc ggcatggggg tccgctccgg gcgattcgag
     7381 aagttgaggt tatgcgagtc cagggaagcc taaagcatca aagtagtcga ctgtaagtat
     7441 acggttcagc ccagtttagc tgtgtctcca ccagtgcccc gcgcctcctg ccataagtgc
     7501 ggcttgctca tggatacccc cgagacggct tacggcgcac cggataataa atttcgcgta
     7561 tacatggtat tccttaccgg agaaattata agcccaacaa caatacgcct gtggcattgt
     7621 tcgctcaaac ttatcgcgag tgatttggct cacactccgg taaagattcc ccatcacgct
     7681 aacggcacat aaacatccgt ttccagtcgc tgttctgtca taccttcggc aagattggtg
     7741 tagtgaaaca gaatcggtgc tttacgcatt ttctcgccgc ttgcaggcag ccagtggcga
     7801 atgatgcccc ataccgtgtg tgaaatatcg tccagctcgc caacgtggcg ggccacggca
     7861 taacgtccac cggtaagttc accattgctc acaccataac gattatcggg aatcggttcg
     7921 ctaacgctgc cgcagatatc aaagcgaaac gctttcggtg cggtggtggc agggtcgtcc
     7981 caggcgacgc caaaagtctg gctttggtta acaggagaaa gccccgtctc tttacgccac
     8041 atgataaatt tcgccacact gtagttgagc aactcagggc tacagcggtg cggcaacatc
     8101 gccacaggga ttgatgggaa atcgataatg tttacatcca gtgtcaggtt tgtcattttg
     8161 cctccctgcg aaattattcc cgggaacgcg tcccgggagc ggtaacaatt acggatttac
     8221 tttgcggatc tgtttgacgt caatttcaac agaattccag tctttatcga cttccccctg
     8281 aatctcaacc gtatctttcg gcgtcaccgt cacaccgttc cagcgtttgt ggtcgatatc
     8341 aacattgata gtaccgctgg catctttaaa cacgtagaga tcgtcagaga tgcgttcaac
     8401 gatattgccg cgcagggtta cccaggtgtc gtcacgcagg gattttgcgc tttctacagt
     8461 cgttacgctg ccgttcggcc cctggaatcc tccggcctga ctttgcgttg ccgatgggcc
     8521 agaaaaaccg ccctgctctg ctgccatcac cggtgcactg cacagggcca ttactgcgat
     8581 tactgctgcg aattttttca tgtttattac tccctttaat gtctgtttcc gagcatttaa
     8641 caagatagtc cttaacaact tcttaaggga aaaaaataaa atttaatgct gtacagagcg
     8701 cgttacaaca cggtttactg gcagcaaata cggttatcgc agggatgaaa aaatgcgaat
     8761 tttactgata gaagatgaca tgctgattgg cgacggcatc aaaactggcc ttagtaaaat
     8821 gggttttagt gtcgactggt ttacacaagg tcgtcaggga aaagaggcgc tttatagcgc
     8881 accttatgat gcggtgatcc tggatttaac cttaccaggc atggatggtc gcgatatttt
     8941 gcgcgaatgg cgagagaaag gtcagcgtga gccggtactg atcctgaccg cgcgtgatgc
     9001 gttagcggaa cgtgtagaag ggctgcgtct gggagctgac gattatctgt gtaaaccttt
     9061 tgcgttgata gaagtcgccg ccaggctgga ggctctgatg cgccgaacca acggccaggc
     9121 cagcaacgag ctgcgccacg gtaacgtcat gctcgacccc ggcaaacgta tcgccacgct
     9181 ggctagcgaa cccttaacac tgaaaccaaa agaatttgcc ctgctggaat tactgatgcg
     9241 taacgctggt cgggtactgc cgcgcaaact gattgaagag aaactgtata cctgggacga
     9301 agaggtcacc agtaatgccg ttgaagtgca tgtgcatcat ctgcgacgca aactcggtag
     9361 tgattttatt cgtaccgtgc atggtattgg ctacacatta ggtgagaaat gaaatttacc
     9421 caacgtctta gtctgcgcgt caggctgacg ctaatctttt taattctggc ctcggtgacc
     9481 tggctgcttt ccagctttgt cgcctggaaa caaacaacgg ataacgtcga tgaattgttc
     9541 gacacccaac tgatgctgtt tgccaagcgg ttaagtacgc tcgatctcaa cgaaatcaac
     9601 gcggcggatc gcatggcaca gacgccaaat aaattaaaac acggtcatgt tgatgacgat
     9661 gcgctgacct ttgccatctt tacccacgac ggcagaatgg tccttaatga tggcgataac
     9721 ggagaagata ttccctatag ctatcaacgg gaaggttttg ctgacgggca actggtcggt
     9781 gaagacgatc cttggcgttt tgtctggatg acctcacctg atggcaaata tcgcatcgtt
     9841 gttggccagg aatgggaata ccgtgaagac atggcgctgg cgattgttgc cgggcaattg
     9901 atcccgtggc tggtcgcact gccgattatg ttaatcatca tgatggtact actgggtcgt
     9961 gaactcgcgc cgctgaacaa actggcgctg gcactacgta tgcgtgaccc tgactcggaa
    10021 aaaccactaa acgcgactgg cgtacccagc gaagtgcgac cactggttga gtcgctaaat
    10081 caactgttcg cccgcacaca tgcgatgatg gttcgagaac gacgctttac ctccgatgca
    10141 gctcacgaac tacgtagccc gttaacagcg ctgaaagtgc aaaccgaagt tgcgcagctc
    10201 tctgacgatg atccgcaggc acggaaaaaa gcactgctcc aattacattc cgggatcgat
    10261 cgcgctactc gtctggttga tcaactgctc acgctatcgc ggctggactc actggataac
    10321 cttcaggacg tcgcggagat cccgcttgaa gatctcctgc aatcgtcggt gatggatatt
    10381 taccacacgg cgcagcaggc gaaaattgac gtgcgactga cactcaatgt ccagggcatc
    10441 aaacgcaccg gacaaccgct attgctaagt ttgttggtgc gaaatttgct ggataacgcc
    10501 gtgcgttaca gtccacaggg cagcgtggtg gacgtcacgc tgaatgccga taatttcatc
    10561 gtgagggata acggccccgg tgtgacacca gaggcactgg cgcgaattgg cgaacgcttc
    10621 tatcgcccct ccggacaaac cgctaccggc agtggacttg ggctatcgat tgtccagcga
    10681 atcgccaagc tgcatggcat gaatgttgag tttgggaatg cggaacaagg tggatttgag
    10741 gcgaaagtaa gctggtaagg gtaagacttt tgctaaattc gcaaaagact ttgcacattt
    10801 tgctaatttc accgtaccgc tctgtgacgt actatagtcg gcaaacgtct caccttgagg
    10861 ttaaaaaatg agcaacattc tgattatcaa cggcgcgaaa aaattcgccc actccaatgg
    10921 tcaactgaat gacaccctga ccgaagtcgc ggatggcaca ctgcgcgacc tcgggcatga
    10981 tgttcgcatc gttcgcgccg acagcgacta cgatgtcaaa gcggaagtac aaaactttct
    11041 ctgggctgat gtggtgatct ggcagatgcc aggctggtgg atgggcgcgc cgtggacagt
    11101 gaaaaaatac attgatgatg tattcaccga aggtcacggg acgctgtatg ccagcgatgg
    11161 ccgtacccgc aaagatccgt caaaaaaata cggttccggc ggcctggtac agggcaaaaa
    11221 atatatgctt tctctgacct ggaacgcacc aatggaagcc ttcaccgaaa aagatcaatt
    11281 cttccacggc gttggcgtcg acggtgtgta tctgccgttc cataaagcaa accaattcct
    11341 tggcatggaa ccgctgccga catttatcgc taatgacgtg ataaaaatgc ctgatgttcc
    11401 ccgctatact gaagaatatc gcaagcatct tgtggaaatt tttggttaac tagagctcag
    11461 gctttagaag gagttaacca tgcttaccgt aatcgcagaa atccgtactc gtcctggtca
    11521 acatcaccgt caggcggtat tggatcagtt tgctaaaatc gttccaaccg tactgaaaga
    11581 agaaggttgc cacggctatg cgccaatggt ggattgcgct gctggcgtga gtttccagtc
    11641 tatggcaccg gattctatcg tgatgattga gcagtgggaa agcatcgcgc accttgaagc
    11701 gcatctgcaa accccgcaca tgaaggcgta cagcgaagcc gtaaaaggtg acgtgctgga
    11761 gatgaatatt cgtattctgc agccagggat ttaatcctgc cttgtttgcc cggccatcct
    11821 gaccgggcaa tgttctttcc tttaaacctc aatctccgcc atgtcgcctt tctcttgcaa
    11881 ccagttgcgg cgatcttccg agcgtttctt cgccagtagc atgtccatca tcgcgtcagt
    11941 acgctgatcg tcttcatcat cgatagtcaa ctgcaccaga cggcgagtgt tcggatcaag
    12001 cgtggtttcg cgcaattgca tcgggttcat ttcccccaga cctttaaaac gctggacgtt
    12061 cggcttgcct ttcttgcgtt ttaattgctc aagtacgccc tctttctctt cttccgtcag
    12121 cgcgtaataa acctctttcc cgagatcaat acggtagagc ggtggcagtg cgacgtaaac
    12181 gtgaccgtgt ttcaccaacg cgcggaagtg ttttacaaac aaagcgcaga gcagcgtggc
    12241 aatgtgcaga ccatcggagt cagcatccgc caggatacag atcttgccgt aacgaagctg
    12301 gctcaaatcg tcgctgtcag gatcgatacc gatcgctacc gaaatatcgt gcacttcctg
    12361 cgaagccagc acttcgtcgg aagagacttc ccaggtgtta aggatcttac ctttcagtgg
    12421 catgatcgcc tgatattcgc gatcgcgcgc ctgcttggca gatccgcctg cggagtcacc
    12481 ttccacaaga aacagttcgg tgcggttaag gtcctgcgcg gtacagtcag ccagtttgcc
    12541 aggcagcgcc ggaccgctgg tcagcttttt acgcaccact tttttagccg cacgcatacg
    12601 acgctgggcg ctggaaatcg ccatctctgc cagcaactcc gccgcctgaa cgttctggtt
    12661 cagccacagg ataaaggcat ctttcaccac gccagaaacg aatgccgcgc actgacgcga
    12721 agagagacgc tctttggtct gcccggcaaa ctgcggatcc tgcattttta ctgacagcac
    12781 ataggcgcag cgatcccaga tatcttccgc cgacagcttt acaccgcgcg gcagaatgtt
    12841 gcggtattca cagaactcac gcatcgcgtc cagcaggccc tgacgcagac cattaacatg
    12901 ggtaccgccc tgcatcgttg ggatcaggtt gacgtagctt tcggtcagca gttcaccgcc
    12961 ttccggcagc cacagtagcg cccagtccac cgcttcagta tcgccagcga aattaccgat
    13021 aaacggtttt tctggcagcg tcggtaaacc gtttaccgct tccgccaggt aatcattcag
    13081 accgtcctga tagcaccagc gctgttcggt gttgttgatc tcatctttaa aggtaatctc
    13141 aacgccgggg cacagtactg ctttggcttt cagcacatgc gtcaggcgtg aaacagaaaa
    13201 acgcgggcta tcgaagaagg tttcatccgg ccagaaatgg acgctggttc cggtattgcg
    13261 tttaccgcaa gtgccgacaa cctgtaaatc ctgcaccttt tcgccatttt caaaggcgat
    13321 gttataaatc tgaccatcgc ggcgcacgtt aacttctacg cgcttcgaca gggcgttaac
    13381 caccgaaatc cccacgccat gcaggccgcc agagaactgg taatttttgt tagagaattt
    13441 accgcctgca tgcagacggc aaagaatcag ttcaaccgcc ggtacaccct cttccgggtg
    13501 aatatccacc ggcatcccgc gcccatcgtc aataacttct aacgactggt cagcatgtaa
    13561 aataacgtcc acgcgttttg cgtgacccgc cagtgcttca tccacactgt tatcaatgac
    13621 ttcttgcccc aaatggttag ggcgagtggt atcggtatac atccccggac ggcggcgaac
    13681 cggctcaagc ccggtgagta cctcaatggc atcagcgtta taagtttgcg tcatggttta
    13741 agttagtaat tcgagttgat cgtcagagat ggtgcagacc aagaaaatcg acgatcgggt
    13801 tgaaataatc ttcgaagccc gtgaatgcgt ggttgccgcc ttctatgaca gtctggcggc
    13861 aggaagcgta gtacgccacc gcctggcggt aatccagcac ttcatctccc gtctgttgca
    13921 gcagccagat caaatccggc gcttccagcg ggtcaatctg catgacttta agatcgtaaa
    13981 tatggcgtga ctctagcaca tattgctgcc cggtgtaggg gttctcgttc tgaccgagat
    14041 agtccgtcag cagttcaaac gggcgcaccg ccgggtttac caccactgcg ggcagcataa
    14101 aacattgtga caaccaggtg gcgtaatatc cccccagtga cgaaccgaca atacccagcg
    14161 aatcgccgcc atgttccagg acaatggatt ccagcagctc tgccgcgtcg gaaggatacg
    14221 gcggcaactg cggaatgatc atctcaacgt cagggtgatg ttccgccagc cagtttttta
    14281 acaagctcgc ttttgcagag cgcggcgagc tgttgaaacc gtgtaaataa agaagcgtag
    14341 acatcagtag ccttctgaag cggtatcagg ttggaaacgt gtgtccgcca ggcgatgcac
    14401 ctcggtggtc agcgtgccat cagcatgtaa ctcgagagta cgccagccgg gcgcgatggt
    14461 atccagcgta aagttggaac agtgcggctt aaactgcaca caggtcgacg gcgttgccag
    14521 caggcggcga ccgttccagt cgagatccag ctcctgatga atatgaccgc acagcaagta
    14581 tttgacgtgc ggaaacttcg ccagcacggt atccagttcg cccgcgttac gcagactgtg
    14641 ttgatcgagc caactacaac ccgcaggtag cggatgatga tgcagcagca gcaacgtatg
    14701 gcgttctggc gcatcggcca gtttacgttc cagccactca agctgaaact cgctcagctc
    14761 accgtgcggc acgccaaaca cctggctatc cagcaacagg atttgccatt gctcaccaat
    14821 aaacacgcgc ttcgccgggg agatacccgc atcctgtaac gcgctgtaca tcgcgggctg
    14881 gaaatcgtgg ttgcccggca gccagacgca gggcgcacga aaacttgcga tgccttcagc
    14941 gaaatgctga taggccgcag aggattgatc ctgcgctaaa tcacctgtcg cgacaatcag
    15001 gtcgaattcg tgctgatgtg gacgaatcgc ctccagcacc gcctggtagc tttcccaggt
    15061 gtttacccct aacagggctt cgtgcttttg tgcaaacagg tgagtatcgg taatttgtaa
    15121 aatcctgact ctggcctcac cagccagagg aagggttaac aggctttcca aatggtgtcc
    15181 ttaggtttca cgacgctagt aaaccggaat cgccatcgct ccatgtgcta aacagtatcg
    15241 caaccagtcc gctaaaaact ggttaatttg atgcttttcg tcgcgttgat gcaacttttt
    15301 attaggataa tcataccgcg ctttgaagcg aaaaatctgc tggcttgaac acacttcagc
    15361 caccatcgcg tcatgataca gacgcaccgt cattgacgga aggctccagt aactgatcgc
    15421 gggcgcagtc tgttctattg tcaccagggt agtgtatcgg gtcgattcca caatcgtcag
    15481 ccgatattgt gcgtttgcca cctgatagct tacagtttcg ccgggtgcgt cattgcgcgg
    15541 taacaaacgg cgcaattgtg aaaagttcat ctcgcacagg cgcatcattt caggaaagtc
    15601 aggtgtgtaa cgcttcattt atgcccactc attttttaac gcttggtggt gcagctgcag
    15661 ccattgcaaa gcgatgaccg acgctgcgtt gtcgattttc ccctcttcta cccactggta
    15721 tgcctgttcc cggcttacca catgaacgcg aatatcttcg ttttcatcag ccagaccatg
    15781 aataccgctt gcggtcgtgg cgtccacttc gcccaccata attgacgaac gctcactggt
    15841 gccccccggg cttgccagga aacttaacac cggtttggtc cgtttgacta tcagccccgc
    15901 ctcttcaatc gcttcgcgac gggcaacatc ttccacactt tcaccctctt caatcatccc
    15961 ggcaaccatc tccagtagcc agggggtttc gctagtgtcg tacgcggcaa ttcgaatctg
    16021 ctcaatcagc acaacttcat cacgcactgg gtcaaagggt agcaagactg cggcgtgacc
    16081 gcgctcaaaa atttcccgcc gcacctcatg actcatttgc ccgttgaata gactatgacg
    16141 aaatctataa agatctaatg aaaaaaagcc gcgataaagt gtttctcgtg caataatttc
    16201 tacatcgttt ttgccaaatg taacgggcag gttgtctggc ttaagcattg ttaatgtcct
    16261 ggcactaata gtgaattaaa tgtgaatttc agcgacgttt gactgccgtt tgagcagtca
    16321 tgtgttaaat tgaggcacat taacgcccta tggcacgtaa cgccaacctt ttgcggtagc
    16381 ggcttctgct agaatccgca ataattttac agtttgatcg cgctaaatac tgcttcacca
    16441 caaggaatgc aaatgaagaa attgctcccc attcttatcg gcctgagcct ttctgggttc
    16501 agttcgttga gccaggccga gaacctgatg caagtttatc agcaagcacg ccttagtaac
    16561 ccggaattgc gtaagtctgc cgccgatcgt gatgctgcct ttgaaaaaat taatgaagcg
    16621 cgcagtccat tactgccaca gctaggttta ggtgcagatt acacctatag caacggctac
    16681 cgcgacgcga acggcatcaa ctcgaacgcg accagtgcgt ccctgcagtt aactcaatcc
    16741 atttttgata tgtcgaaatg gcgtgcgtta acgctgcagg aaaaagcagc agggattcag
    16801 gacgtcacat atcagaccga tcagcaaacc ttgatcctca acaccgcgac cgcttatttc
    16861 aacgtgttga atgctattga cgttctttcc tatacacagg cacaaaaaga agcgatctac
    16921 cgtcaattag atcaaaccac ccaacgtttt aacgtgggcc tggtagcgat caccgacgtg
    16981 cagaacgccc gcgcgcagta cgataccgtg ctggcgaacg aagtgaccgc acgtaataac
    17041 cttgataacg cggtagagca gctgcgccag atcaccggta actactatcc ggaactggcg
    17101 gcgctgaatg tcgaaaactt taaaaccgac aaaccacagc cggttaacgc gctgctgaaa
    17161 gaagccgaaa aacgcaacct gtcgctgtta caggcacgct tgagccagga cctggcgcgc
    17221 gagcaaattc gccaggcgca ggatggtcac ttaccgacgc tggatttaac ggcttctacc
    17281 gggatttctg acacctctta tagcggttcg aaaactcgtg gtgccgctgg tacccagtat
    17341 gacgacagca atatgggcca gaacaaagtg ggcctgagct tctcgctgcc gatttatcag
    17401 ggcggaatgg ttaactcgca ggtgaaacag gcccagtaca actttgttgg tgccagcgag
    17461 caactggaaa gcgcgcatcg tagcatcgtg caaaccgtac gttcctcctt caacaacatt
    17521 aatgcatcta tcagtagcat taacgcctac aaacaagccg tagtttccgc tcaaagctca
    17581 ttagacgcga tggaagcggg ctactcggtc ggtacgcgta ccattgttga tgtgttggat
    17641 gcaaccacca cgctgtacaa cgctaagcaa gagctggcaa atgcgcgtta taactacctg
    17701 attaatcagc tgaatattaa gtcagccctg ggtacgttga acgagcagga tctgctggca
    17761 ctgaacaatg cgctgagcaa accggtttcc actaatccgg aaaacgttgc cccgcaaacg
    17821 ccggaacaga atgctattgc tgatggttat gcgcctgata gcccggcacc cgtcgttcag
    17881 caaacatccg cacgcactac caccagtaac ggtcataacc ctttccgtaa ctgatgacga
    17941 cgacggggct tcggccccgt ctgaacgtaa gacaacgtaa agatacgggt tatctgccgc
    18001 attcttcccc cttctcgctt caatttcgac cagccatcct ctattctgat gggtatttac
    18061 cactggtccc ggaagacaaa aatgaaacgg acaaaatcca tacgccacgc atcgttccgc
    18121 aaaaactgga gcgcacgcca tctgacacca gtcgctctcg cggttgccac tgtttttatg
    18181 ctggctagct gtgaaaagag tgatgaaaca gtgtctctct atcaaaatgc tgacgactgt
    18241 tcagctgcaa acccaggcaa aagcgccgaa tgtaccaccg cgtacaacaa tgcgctgaaa
    18301 gaagccgaac gtactgcgcc gaaatacgcc acccgtgaag actgtgttgc tgaatttggt
    18361 gaaggtcagt gccagcaggc accagcccag gctggcatgg caccagaaaa ccaggcgcag
    18421 gcccagcaat ccagcgggag tttctggatg ccgctgatgg ccggttacat gatggggcgt
    18481 ctgatgggcg gcggcgcggg atttgcacag cagccgctgt tctcctcgaa aaacccagcc
    18541 agcccggctt acggtaaata taccgacgcg acgggtaaaa actatggcgc agcccagcca
    18601 ggccgcacca tgaccgtacc gaagacagcc atggcaccaa aaccggcgac cactactacc
    18661 gttacccgtg gcggttttgg tgaatctgtt gccaaacaaa gcactatgca gcgtagcgca
    18721 accggcacct cttctcgttc aatgggtggc tgataccgat ggaaagagtc agtattaccg
    18781 agcgcccgga ctggcgtgaa aaagcccacg aatacggttt caattttcac accatgtacg
    18841 gcgagccgta ctggtgtgaa gatgcttact acaagttgcc cctcgcccag gttgaaaagc
    18901 tggaagaagt caccgccgaa ctgcaccaga tgtgcctgaa agtggtggaa aaagtgatcg
    18961 ccagcgatga gctgatgacc aaattccgca ttccaaaaca cacctggagt tttgtgcgtc
    19021 agtcatggct gactcaccag ccatcgcttt attcgcgtct tgatctggca tgggatggca
    19081 ctggcgaacc taaactgctg gaaaataacg ccgatacacc aacgtcacta tacgaggcgg
    19141 cgttctttca gtggatctgg ctggaagatc agcttaacgc cggtaacttg ccggagggca
    19201 gcgaccagtt taacagtctg caagaaaagc taatcgatcg ctttgtcgag ttgcgtgaac
    19261 agtatggctt ccagttgctg catctcacct gctgtcgcga cacggtggaa gatcgcggaa
    19321 ccattcagta tttgcaggac tgcgcgacgg aagctgaaat cgctaccgag ttcctctaca
    19381 ttgacgatat cgggttaggt gaaaaaggtc agttcacgga tttacaggat caggtaattt
    19441 ccaacctgtt caagctgtat ccatgggaat ttatgttgcg tgagatgttc tccaccaagc
    19501 tggaggatgc aggcgtacgc tggctggaac cggcgtggaa gagcattatc tccaacaaag
    19561 cgcttctacc gctactgtgg gagatgttcc cgaatcaccc gaacctgctg cccgcttatt
    19621 ttgcggaaga tgatcatccg caaatggaaa aatatgtggt taaaccgatc ttctcccgtg
    19681 aaggcgcaaa cgtgtcgatc attgagaacg gcaaaaccat tgaagcagcg gaaggtccgt
    19741 atggcgaaga agggatgatt gttcagcaat tccatccgtt accgaaattc ggcgacagct
    19801 atatgctgat tggtagctgg ctggtgaacg atcaacccgc cggaattggc attcgtgaag
    19861 accgtgcatt gatcacccag gatatgtctc ggttttatcc acatattttt gttgagtaat
    19921 caatgatgcc ggatggcatt cgccatccgg taattgttaa cctatctgca ccgacagcat
    19981 actcaggctg cccatttcta taccatcgac cggaatggta attggctcct gcccatccca
    20041 cgcacctaag acatacaaca acggcagata gtgctccggc gttgggttcg ataacgcgcc
    20101 accttcatgg tcgaggtaat tcaccagagg atgctgctct accgcccctt gccacgtcag
    20161 attcgctttc acatactcat taaacgacat cgcccaagga tatggtgaac tatcaccgtg
    20221 ccacttcact gtacgcaggt tatgcaccac gttgccactg gcgaccaaca ttattccttc
    20281 atctcgcagc gctgccagtt tgcgccccat ttcgaaatgc caggcggcag gtttgctact
    20341 gtcgatactc aactgcacca tcgggatatc ggcgtcagga tacatcttaa tcagcacgcc
    20401 ccaggaaccg tggtcaaagc ccctggcttc tttatccagc gtcaccggga ccggcgctaa
    20461 cagctcaacc agatgctgtg ccagcacagg cgaacccgga gcaggataat gcgtatcgta
    20521 cagcgcctgc gggaagccac caaagtcatg aatcgtgggc ggcgtctcca tcgcggtcac
    20581 tcctgttcca cgggtaaacc agtgagccga aaccaccaca atcgcttgcg ggcgtggcaa
    20641 tgtcatcccc aacttctgcc agctgcgggt atacaaatta tcttccagca cgttcatcgg
    20701 actaccgtga cctaaaaaca atgctggcat acgtgttgaa gacatgatga tatccttaac
    20761 taagggtgtc attttgatat cctcacaata cgcttgttcg gcggagtaag aacccggata
    20821 acaatgatga tgatcatcag ttattttgac gatctgcctg aaggtgaaga tttataagga
    20881 gttgtcgatg tcagtacctc tcattctgac catactggcg ggggcagcca cgtttattgg
    20941 cgcgtttctc ggcgttctcg gacaaaaacc ctcgaaccgc ttactggcgt tttcgctagg
    21001 ttttgcggcg gggatcatgt tgctcatctc attaatggaa atgcttcctg ccgcactagc
    21061 agctgaagga atatcgcctg tgttgggtta tggaatgttt atcttcggtc tgcttggcta
    21121 ctttggcctg gaccgcatgt tgccgcatgc gcatccgcag gatttaatgc aaaaatcggt
    21181 gcagccgttg ccaaaatcga tcaagcgcac agccattctg ctcactctcg gcatcagtct
    21241 gcataacttc ccggaaggga ttgccacctt tgtcacggcg agcagcaacc tggagctggg
    21301 atttggcatc gcactggccg tcgcgttgca caatatccct gaaggtctgg cagtggcagg
    21361 cccggtttat gcggcaacgg ggtctaaacg caccgcgatt ctgtgggcgg ggatttctgg
    21421 actagcagaa attcttggtg gtgtgctggc gtggttaatc ctcggtagca tgatttcccc
    21481 ggtggtcatg gcggcggttg caggaattat ggtggcgctc tcggttgatg aattaatgcc
    21541 gctcgccaaa gagattgacc ctaataataa ccccagctat ggcgtactat gtggaatgtc
    21601 agtgatggga ttcagtttag tgctgctaca aacggcggga attggttaaa aacaacatca
    21661 gaagttgtcc agcacatgga caacttctgc cagcttcacg aaagccttat tctacctttt
    21721 cggctgtctc ttctctcgta ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc
    21781 tgtcctggcg aaacgcccga caataacgtt tcaaatgcag gaaataaccc accacaacca
    21841 gagcaatcaa tacaatccag taccaggcga taaacatcct ttacccttaa tatttaacaa
    21901 tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc atatggtcgc
    21961 ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct gatattaatg
    22021 aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc aaaataaaaa
    22081 ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag cggttttcag
    22141 ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt cacgagcgcc
    22201 atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt gccatcgtca
    22261 ttagtcagct cacacagcac accagccggt ttaaagcctg ccagcgtcat caaatcaata
    22321 gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg aagtgggaaa
    22381 acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc tgcgcgaacg
    22441 gtcgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc ttcaatggtc
    22501 acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg cagatcaagt
    22561 tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg gcgaatggtc
    22621 agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc ttcgttttca
    22681 cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc cagtgcattt
    22741 tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat ggtaaaaaaa
    22801 actcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg catatagcca
    22861 aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc cggactctaa
    22921 ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg
    22981 cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc ctgagaataa
    23041 gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac ataattctgg
    23101 tttatgactt acccttatcg cactacaatg gtactcaaca cctatcagta cagggaaact
    23161 acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aagttcacga atcaatgcct
    23221 aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct
    23281 cagctgacgc gcctcgacct tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg
    23341 ttacggactc gtgagaaact ggcgctgctt gaacaaagaa tgagcgaact ggaaaatcgc
    23401 agtactgaga ttaaaaagca gccagatcca gagactctcc ccccaacgct gtaaacattt
    23461 gtctttaatg cactgtaaat tatgaattaa tcttctaatt tacagtgctt tctattacct
    23521 tatcgcttaa ttccattcag aatatcacca caaaaatgaa tgagtgcaat tgaaaatata
    23581 attgttatgt atttttttaa gttttactta atccatattt agataaacta atttcataaa
    23641 acccgaaaag taaaatataa tatttgaatg attagcaatc cttaaataac agttactaat
    23701 cattcatttt ctacttatat ttatataaag gaatattatg tctgctttta aaaagtcact
    23761 tcttgttgct ggcgtagcaa tgattctttc caataacgta tttgctgacg aaggtcacgg
    23821 cattgttaaa tttaaaggtg aagttatctc tgctccatgt tcaattaaac caggtgacga
    23881 agacttaact gtaaatctgg gtgaagtagc agataccgtt ctgaaaagcg accagaaatc
    23941 tttggctgag cctttcacta ttcacctgca agactgcatg ttgagccagg gtggaaccac
    24001 ttattcaaaa gcgaaggtta ctttcaccac agcgaatact atgacgggtc agactgacct
    24061 gctgaaaaac accaaagaaa ctgaaatcgg tggtgccaca ggcgttggcg tacgtattct
    24121 gaatagccag agcggcgaag tgactttagg tactccggta gtaattacat tcaacaatac
    24181 taacagctat caggaactga acttcaaagt tcgtatggaa tccccgggca aagatgctac
    24241 cccgggcaac gtttacgctc aggctgacta taagattgct tacgagtaat cacattaggc
    24301 cgggttcgcc cggccacatc attaacggat taatgataag tggaacagat gtataaaaaa
    24361 ttaaaattaa ccacaataag cgaattgatt aaaaatattt attgttcatt atccgttctc
    24421 atcattggtt gtgcgtcagc ttatgccgtt gaattcaaca aagatttaat cgaagccgaa
    24481 gatcgtgaaa acgttaacct ttcccaattt gaaactgatg gccaattacc cgtaggtaaa
    24541 tactcattaa acgctattat taataataaa aggacgccaa tccatctgga cctgcaatgg
    24601 gtattaattg ataaccaaac tgcagtttgt ctgactccag agcaattaac attattaggc
    24661 tttactgatg agattattga agaggctcag caaaacctga tcgatggttg ttaccctatc
    24721 gaaaaagaaa aacaaattac aacttatctc gataaaggga aaatgcaatt atccatatct
    24781 gcacctcagg catggttaaa atacaaagat gcaa
//
LOCUS       DAJVOY010000046        24658 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVOY010000046 DAJVOY010000000
VERSION     DAJVOY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.046"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..421
                     /gene="speG"
                     /locus_tag="OQL90_001657"
     CDS             <1..421
                     /gene="speG"
                     /locus_tag="OQL90_001657"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCS1368476.1"
                     /translation="TTSHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIIS
                     PEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHE
                     FFINGQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(424..1134)
                     /locus_tag="OQL90_001658"
     CDS             complement(424..1134)
                     /locus_tag="OQL90_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCS1368477.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            1242..1547
                     /locus_tag="OQL90_001659"
     CDS             1242..1547
                     /locus_tag="OQL90_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCS1368478.1"
                     /translation="MKLSTCCAALLLALASPAVLATPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQSTQDGAPAEPQ"
     gene            1746..2312
                     /locus_tag="OQL90_001660"
                     /pseudo
     CDS             1746..2312
                     /locus_tag="OQL90_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            2294..2422
                     /locus_tag="OQL90_001661"
                     /pseudo
     CDS             2294..2422
                     /locus_tag="OQL90_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
     gene            2465..3079
                     /gene="dmsD"
                     /locus_tag="OQL90_001662"
     CDS             2465..3079
                     /gene="dmsD"
                     /locus_tag="OQL90_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1368479.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            3238..4530
                     /gene="clcB"
                     /locus_tag="OQL90_001663"
     CDS             3238..4530
                     /gene="clcB"
                     /locus_tag="OQL90_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1368480.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4483..5178)
                     /gene="bioD"
                     /locus_tag="OQL90_001664"
     CDS             complement(4483..5178)
                     /gene="bioD"
                     /locus_tag="OQL90_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1368481.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(5303..6523)
                     /gene="mlc"
                     /locus_tag="OQL90_001665"
                     /gene_synonym="dgsA"
     CDS             complement(5303..6523)
                     /gene="mlc"
                     /locus_tag="OQL90_001665"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1368482.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPIISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            complement(6658..7551)
                     /locus_tag="OQL90_001666"
     CDS             complement(6658..7551)
                     /locus_tag="OQL90_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1368483.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            7658..8910
                     /locus_tag="OQL90_001667"
                     /pseudo
     CDS             7658..8910
                     /locus_tag="OQL90_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9307..9621
                     /locus_tag="OQL90_001668"
     CDS             9307..9621
                     /locus_tag="OQL90_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS1368484.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            9714..9797
                     /locus_tag="OQL90_001669"
     CDS             9714..9797
                     /locus_tag="OQL90_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368485.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            9897..10718
                     /locus_tag="OQL90_001670"
     CDS             9897..10718
                     /locus_tag="OQL90_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1368486.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(10757..11086)
                     /gene="mdtI"
                     /locus_tag="OQL90_001671"
     CDS             complement(10757..11086)
                     /gene="mdtI"
                     /locus_tag="OQL90_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1368487.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(11073..11438)
                     /gene="mdtJ"
                     /locus_tag="OQL90_001672"
     CDS             complement(11073..11438)
                     /gene="mdtJ"
                     /locus_tag="OQL90_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1368488.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            11850..12884
                     /gene="tqsA"
                     /locus_tag="OQL90_001673"
     CDS             11850..12884
                     /gene="tqsA"
                     /locus_tag="OQL90_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS1368489.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(12909..14297)
                     /gene="pntB"
                     /locus_tag="OQL90_001674"
     CDS             complement(12909..14297)
                     /gene="pntB"
                     /locus_tag="OQL90_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1368490.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(14308..15876)
                     /gene="pntA"
                     /locus_tag="OQL90_001675"
     CDS             complement(14308..15876)
                     /gene="pntA"
                     /locus_tag="OQL90_001675"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1368491.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAPPIQVSAQPQ
                     AAQKAAPEVKTEEKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVG
                     YYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGG
                     FTVTQRMLKMFRKN"
     gene            16400..17344
                     /locus_tag="OQL90_001676"
     CDS             16400..17344
                     /locus_tag="OQL90_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1368492.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            17530..18912
                     /locus_tag="OQL90_001677"
     CDS             17530..18912
                     /locus_tag="OQL90_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1368493.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWFVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            18949..19671
                     /gene="folM"
                     /locus_tag="OQL90_001678"
     CDS             18949..19671
                     /gene="folM"
                     /locus_tag="OQL90_001678"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1368494.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(19668..19980)
                     /locus_tag="OQL90_001679"
                     /pseudo
     CDS             complement(19668..19980)
                     /locus_tag="OQL90_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
     gene            20106..20825
                     /gene="rstA"
                     /locus_tag="OQL90_001680"
     CDS             20106..20825
                     /gene="rstA"
                     /locus_tag="OQL90_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1368495.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            20829..22130
                     /gene="rstB"
                     /locus_tag="OQL90_001681"
     CDS             20829..22130
                     /gene="rstB"
                     /locus_tag="OQL90_001681"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1368496.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            22206..23135
                     /gene="tus"
                     /locus_tag="OQL90_001682"
     CDS             22206..23135
                     /gene="tus"
                     /locus_tag="OQL90_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1368497.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHCDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(23132..24535)
                     /gene="fumC"
                     /locus_tag="OQL90_001683"
     CDS             complement(23132..24535)
                     /gene="fumC"
                     /locus_tag="OQL90_001683"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1368498.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         5950 a         6350 c         6028 g         6330 t
ORIGIN      
        1 gaccacttca catattcacg atcagagcga acgacgcttt gtggtggaat gtgacggcga
       61 aaaagccggt ctggtggagc tggtggaaat taaccatgta catcgccggg cagaatttca
      121 gataattatc tccccggagt atcaggggaa aggtctggca acccgtgccg ccaaattagc
      181 aatggactat ggcttcaccg ttctcaatct ctataagctg tatctgatcg tcgataaaga
      241 gaatgaaaaa gcgattcaca tttaccgcaa gcttggcttt acggttgaag gtgaattgat
      301 gcatgagttt tttattaatg gtcaatatcg taatgccatt cgcatgtgta tattccagca
      361 tcagtatctg gcagagcaca aaacaccggg tcagactctc ctgaagccga ccgcacaata
      421 gcattaataa taatcgatcg tatttttgat ggtgtaaacc cgttcgacgg cgggttttac
      481 tccttcatca acaatgatca gctgacagtc caccggattg gcgtgactgt catattcaca
      541 gctctgtttt acattaccaa ctcgttgatt attcagtaaa gtaaccgctg tgtaatctaa
      601 ttttttgatc gggtccgttg atggcgtggc gctgacagat aatgttttgt cgttactttt
      661 cgtggtttta cccagtggat aaccctgatc atcatagcga tattccatct gcatttgttt
      721 gctgctggct ttaatcacga agccattatc atcggtttcc caactcaccc cggcagaagg
      781 taattctgct agctggcatt ttccctgtaa acgtactctc ttctccagcg tctcggcatc
      841 acggtaataa ttggcgtcca gtaccagagc gaccacggta ttattttcca gatccagtaa
      901 ttcgagtgaa tcaaaacagc cttcttccga caaagtccca gaaacacgtt tcgtcacttc
      961 accttgctca tccattaatg tctgagtgaa atcttttacc ggaccacgca gcggatcaaa
     1021 atcgaattca ttagagaaac tggccatctc aggggtaaat gaaagtgtta cctctgtgcg
     1081 gtcacatcct gtgaggaata tcgcgagtaa gcatggtagt aatttgtatt tcacaacagt
     1141 caccagagag tagagatgat tctcaatcat agtagcaaat acagtacttt acacgttaaa
     1201 tgctatgctt aaagaagtta tcttcgcgta aggagcttat gatgaaactc tcaacctgct
     1261 gtgccgcgct tctgctcgcc ctcgcctcac ccgcggtact cgccacgccg ggatcctgtg
     1321 agcgcataca gagcgatata tcacagcgca ttatcaataa tggcgtaccc gaaagcagct
     1381 tcacattaag tattgtaccc aatgaccagg ttgatcagcc tgattcccag gtcgtcgggc
     1441 attgtgctaa tgatacgcat aaaattctct acacccgcac caccagcgct aacgtctctg
     1501 ctcccgcgca gtctacccag gatggcgcgc ctgccgaacc gcagtaatac attatcgact
     1561 gaacgccgga tatgacacat ccggcgattt gaacatacaa cataatccca ccttattact
     1621 catacccttc tattgatgtg gattaataat tcttaaccca aaatgggtag accccctcta
     1681 ttgttagcgc gctaaatatt caatacataa acatttatat aacgataaag aacagggagt
     1741 gagttatgtc caaaaatgac cgaatggtgg gcatcagccg cagaacactc gttaaatcaa
     1801 ccgcgatagg ttctctggcg ctggctgcag gcggtttttc tttaccgttc accctgcgca
     1861 gtgcagcggc aacggtacaa caggccagcg aaaaagtgat ctggggtgcc tgttccgtca
     1921 actgtggtag ccgctgtgca cttcgtctac atgttaaaga taatgaagtg acctgggtgg
     1981 aaactgacaa taccggcagc gatgagtacg gcaaccatca ggtacgcgcc tgtttgcgcg
     2041 gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaattaccca atgaaacgcg
     2101 tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc ctggatacta
     2161 tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaggctgta tatattcagt
     2221 actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc tcggcggtca
     2281 aacgcctgat gaactgctac ggcggttccc tcacgttgcc ggacttattc tggggctaat
     2341 cctcatactt ggcggtgaga tgatcgggcg tgtgctcttt tatggcctgc acatgacagt
     2401 aggaatggct attgcaggtt aacaaattgg tgcggggcaa cccgcacatt tcaggatgtt
     2461 aggaatgacc catttttcac agcaagataa tttttctgtc gcggcgcgcg tgttgggcgc
     2521 gctgttttat tacgctccag agagtgcgga agccgcacct ctggttgcgg tactcaccag
     2581 tgacggctgg gaaactcagt ggcctctacc agaagcgtca ttagcgccgc tggtgacggc
     2641 ttttcagaca cagtgtgaag agactcacgc ccaggcctgg cagcgtttgt ttgtcggccc
     2701 gtgggcactg ccgtctccgc cgtgggggtc ggtctggctg gatcgcgaat ctgtgctgtt
     2761 tggcgattca acattggcac ttcgtcagtg gatgcgcgag aaaggcattc agtttgaaat
     2821 gaagcaatac gaaccggaag atcattttgg ttcactgctg ttgatggctg cgtggttggc
     2881 ggagaatggt cgccagacag aatgcgaaga attgctggca tggcaccttt ttccgtggtc
     2941 aacacgtttt cttgatgttt ttatcgaaaa agcagaacac cctttctacc gtgcactggg
     3001 tgaactgtct cgcttaacgc tggcgcaatg gcaatcacaa ctgttaattc ctgtcgcggt
     3061 taaaccgctg tttcgatagg atcaccgtaa tattgccgga tggtgacgat tacgcgcaat
     3121 ccggcaataa taggttacag tgtcacgttt ttttatctct taaagcacgc actgcttttg
     3181 cggctggcct cttttgccgc aaaatagtcg cccgtgtttc attgcccatt tctgctcatg
     3241 catcatctac acatctatcc ggatctgcgc actatgttcc gccgtctgct tatcgcaaca
     3301 gtcgtcggta ttctcgcggc ctttgccgtt gccgggtttc gtcatgcgat gctgctactg
     3361 gagtggttgt tcctcaataa tgactccggc agtctggtca atgcagcgac aaacctttcc
     3421 ccctggcgac ggttgctaac tccggcgctc ggcggactgg cggcgggttt gttgctgatg
     3481 ggctggcaga aatttaccca acaacgccct catgcgccga ccgattacat ggaagcgttg
     3541 caaaccgatg gacagttcga ttacgcagca agcctggtta aatcgcttgc ctcgctgctg
     3601 gtagtaacca gcggcagtgc aattggtcgc gaaggtgcga tgattctttt agctgccctt
     3661 gccgcctcct gttttgccca acgttttacg ccacgccagg agtggaaatt atggatcgcc
     3721 tgtggcgccg cggcgggaat ggctgcggcc tatcgtgccc cgcttgctgg cagtttattt
     3781 atagccgaag tgctgtttgg cactatgatg ttggcctctc tcggcccggt gattatttcc
     3841 gccgtcgtgg cattgctggt tagcaatctg attaatcata gcgacgcgtt actctacaac
     3901 gtacaactct cagtgacggt tcaggctcgt gactatgcgc tgattatcag tacaggtgtg
     3961 ctggcaggtc tgtgcggacc actgttgtta acgttaatga acgcctgtca tcgtggattt
     4021 gtgagtctca aacttgcgcc gccctggcaa ctggcactag gcgggttgat cgtgggtctg
     4081 ctttccctgt tcacacctgc agtgtggggc aacggctata gcaccgtaca atccttttta
     4141 accgccccac cactgttaat gatcattgcc gggatcttcc tttgtaaact gtgtgccgtg
     4201 ctggcgagta gcggttccgg cgcacccggt ggtgtcttta caccgacgct atttatcggt
     4261 cttgccattg gcatgttgta tggtcgtagc ctgggattat ggttccctga tggcgaagaa
     4321 attacgcttt tactcggatt gaccgagatg gcgacactgt tggcggcaac cacgcacgcg
     4381 ccgattatgt cgacgttgat gatatgtgaa atgaccgggg agtatcagct actccccggt
     4441 ttattgattg cctgcgtcat tgcgtcggta atttcgcgga cgttacaccg tgactctatc
     4501 taccgccagc acactgcgca gcatagctaa gcggatgtat tgccccagtt ctcgctgttc
     4561 ggcgcgcggc agataaggca gttcaccaat gagcggtgcc ggaagttttt tacccagcac
     4621 atcaatgatt tccgcataat gcgccagtcc tgggttgatt cggttagcca cccagccaat
     4681 gagcggcagc ccgtcgttgg caatcgcctg agctgttagc agtgcatggt taatgcaacc
     4741 ttcctgaata ccgacaacca tcaacaccgg caactgttcc tgcaccaccc attcagagag
     4801 tggacgcaaa tcattcatca gactgcgcca gccgccagtc ccttctacca cgacatgatc
     4861 gactttatcg gtcaggtttg ccaggccgtt tgaaatgagg gtgtaattga ttgggcaact
     4921 gtgcgccacg ctactttctt cttcgcttaa cgcgatagga ttaactgctt cataaggtag
     4981 ttcgatggtt gaaacactct gcaacaccag ggcatcttta ttacgcagcc cttcgggtgt
     5041 ctctttgctc cccttcgcta cgggtttata tcccgcaact gtttttccct gggaggctaa
     5101 cgcttgtagc aatgcgcggg aaaccaccgt tttccctaca gaagtgtctg taccggtaat
     5161 aaagaaacgc ttcagcatca ctaactccac cgttatgctt cacaaatata aactaggaaa
     5221 ataattaacc ttgaaagtct aagttatgct ttcctggccc aaattgagat agcgcaaatt
     5281 ttggtagaac agttaaaaaa tgttaaccat gcaacagacg aatcaacaaa gaaccgttat
     5341 acatcgcgtc ttttaccagc gcagcgcctg ccatcgtgcc ctggttagaa aactgagtac
     5401 tctcaacgct aatgtgctga ctatacgtag gaagggcctg ctgacgtatg ctgtccgaga
     5461 tgatagggaa gaggatatct gccgctttac ttaacggtga gccaatcagt attttttgtg
     5521 ggttaaataa attcaccatg atggcaagaa tgcgcccgac atgcgcgccc accccggtaa
     5581 tgatgtcttt tgccagtaga tcgccgcgca atgccgcctg acacaatgag tccacggtta
     5641 acggttgccc atgtaacatc gagctcatgg attgattgag acgcagctgt gccagctcaa
     5701 gaatactgtc cacactggcg atggtttcga ggcagccgag gttcccgcaa taacagcgtt
     5761 taccatatgg gtcgacctgt gtgtggccta tttccacgag actactgctg cctgcgtgta
     5821 gcagatgacc atcggtaatg acgcccgccc ccacgttgtg atcgataacc acctgaatca
     5881 catcgcgcgc cccgcgtgag gcaccaaaca aggcctctgc catcgtccat gcgctgatat
     5941 catgctgaat ataaaccggt acgccggtat gctgctccag cgcctcgccg agcggcatct
     6001 cttttacatc ctcgtagaac ggcatgcgat gtacaatacc attttccgta tcaataattc
     6061 ccggcagggt tatggcaatc gaagttagac gctcaagttt tttctggtgg cggataaaaa
     6121 actgatcgat atgggaaatg atacgatcca gcaatggcaa gtcatctttt aacgccagtt
     6181 cctgcgactc ttccaccacc agtttgctgc tcagatcgcg cagagcaagg aaaatctcac
     6241 cgcgactaat gcgcagagaa agatagtgcc aggcttcagt ttcaaccacc agtcccaccg
     6301 ccggacggcc acggttcccc gcttctttga tttccagctc ttgcaccaag tgtgcttcga
     6361 gcatctcacg gacaatttta gtgatactgg caggagccag ctgcgccaga cgggaaagat
     6421 cgatacgcga gactggacca agctgatcaa tcaggcgata aaccgcgccc gcgttggtct
     6481 gctttatttg atcaatgtgc ccaggctggt tttcagcaac caccgcatac tccctatatt
     6541 ttcgcgctcc gaaataatct gtaggctatg gtgaagcact tcaatacgtg tcgtcaaatt
     6601 tttacttaga catgtgatta acagcacatt tttcgggctt tttcgctgaa atttccctca
     6661 cctgagagca ttcagcagat gaatacgaaa gttacgcgaa gctggacttt gttcatgatg
     6721 tttcggccag accaaccaca tttcagaaac cgcatcctct tcagcaatcg gcacccagcg
     6781 catttcgttg agctgagcac gtttaaatga agcaggcaaa attgaaacac ccagaccggc
     6841 ggaaaccaga ccgatgatgg tcattgcctc gcccacctcc tgagtgatga cgggcgtcaa
     6901 atggtaacgt cgcatcagcc cgagaatatc gtcatacagc cctgtcccga cgtgcggatc
     6961 aaaaaagacg aagggttctt tcgccagttc agccaacgtt acattcgggt tatttgccag
     7021 gggatgatcg tgcgggatca tcgccataag cggttcatgg acgatgactg cgtgttcaag
     7081 cgtctccggt aacgctgtgt tacgcagcaa tcccatatcc agcgttcctt caatgagcgg
     7141 agcgatttgc tcacgagtgt tcatttcgcg ggtttgtaaa tggacaccgg gataatcacg
     7201 gcgaaacagc gataacgtat cggacaccgc ccgaataaaa ggagcggacg aagtaaaacc
     7261 aatgcgcaac tcccccgctt caccctgatg cagtctttca gcgcgagcgg cagcgtcatc
     7321 taccatagac aggatttgcc gactatctgc aagaaactgt tttcctgctg ccgtcagcaa
     7381 tacactgcga ttagttcgtg ccagcagtcg ggcaccaatt tgttgctcca gtgcctgaat
     7441 ctgctgactt agcggtggtt gcgaaatatt caggcgggca gcggcgcgcc cgaaatgcag
     7501 ctcttccgca acagcaacaa agtaacgcag atgacgaagt tcaatattca tatttaaaac
     7561 atcttatttg agattattaa tatattagac agaacaattc gattttccta acctatgtat
     7621 aagcctgatc cacaggcata tttagcaagg atttcaagtg agccgtacta caactgttga
     7681 tggcgctccg gcaagcgaca ctgacaagca aagcatttct cagccaaatc aatttattaa
     7741 acgcggtacg ccgcaattta tgcgcgtcac cctggcgctg ttctctgccg gactggcaac
     7801 ctttgcactc ctctattgtg tgcagcctat ccttccggtg ctttcgcagg agtttggctt
     7861 aacccccgcg aacagtagta tttcactgtc catttccacg gcgatgttgg ctattggttt
     7921 gctgtttact ggccccctat ccgatgccat tggtcgcaaa ccagtgatgg tcacggcgct
     7981 actgttggcc tcaatttgta cgttatttcg acaatgatga ccagctggca cggcattttg
     8041 attatgcgcg ccttgattgg gctttcgtta agtggcgtgg cagctgttgg catgacttat
     8101 cttagcgagg aaatccaccc cagtttcgtg gccttttcga tggggttgta tatcagcggc
     8161 aactcaattg gcggcatgag cggacgctta attagtggtg tcttcacgga ctttttcaac
     8221 tggcgaattg ctctggcggc aatcggttgt ttcgcgctag cctcggcgtt gatgttctgg
     8281 aaaatccttc cggaatcacg ccattttcgc ccgacttcgc tgcgacctaa gacgttgttt
     8341 atcaactttc gtctgcactg gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt
     8401 ttgctgatgg ggtcgttcgt cacgctgttt aattacatcg gctatcggtt gatgctttcc
     8461 ccctggcatg tcagtcaggc cgtggttggc ttattatcgc tggcttattt gaccggtaca
     8521 cggagctcac ccaaagccgg aaccatgacc acccgctacg ggcgcggtcc ggtgatgttg
     8581 ttttcgacgg gggttatgct gtttggttta ctgatgacct tattcagctc gctgtggctg
     8641 atctttgccg gaatgttact cttctcagca ggattcttcg cagcccactc agttgccagc
     8701 agctggatcg gcccccgcgc aaaacgcgct aaaggccagg cctcctcgct gtatctgttc
     8761 agttactatc tgggatcgag tattgccggg acgctgggtg atgttttctg gcataactat
     8821 ggctggaacg gcgtcggcgc atttattgct ctgatgctgg tcattgctct gctggtcggg
     8881 acgcgtttgc atcgtcgtct gcacgcctga aaaataagcc cggactgcgg taactacacg
     8941 tccggactta ttgcttgctc aaaccaacgt taatagccat cctaaaatag acgaagcgcc
     9001 agccaattcc cgcagtgcgt ctagcgtcat caggattata agtacccaaa taaacggatt
     9061 cattttgctg tgtgtcattt attactgatg cgcagttatt ctaccgcttt gtaagtagta
     9121 aaatagttaa cccgatcaag actactatta ttggtagcta aatttccctt aagtcacaat
     9181 acgttattat caatgctgta atttattcag cgtttgtaca tatcgttaca cgctgaaacc
     9241 aaccactcac ggaagtctgc cattcccagg gatatagtta tttcaacggc cccgcagtgg
     9301 ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa aagtattagc tctggttgtt
     9361 gccgctgcta tgggtctgtc ttctgccgcc tttgctgcag agactgcgac gaccaccaaa
     9421 gcagcgccgg cgaaaacgac acatcataaa aaacagcata aagcagcacc tgcccagaaa
     9481 gcgcaggcag ctaaaaagca tcataaaaat acgaaagctg aacagaaagc ccctgaacaa
     9541 aaagcgcagg cagcgaagaa acacgccggg aaacacagtc atcagcaacc ggcaaaacct
     9601 gctgcacaac ccgcagcgta agttttcaac agtaatgctg gcgcgccccc tcgcgcctga
     9661 aaattacggt gctaagcggg taacgtttag caccgccttt agcggagggt agtatggtgg
     9721 gccgttatcg ctttgagttc attcttatca tccttatttt atgcgcactg attgccgccc
     9781 gtttttatct ttcctgactg tagttatctg attttactcc cactttcatc ccgtcccgtc
     9841 tatagtattt atgagggttt gcttttaata atcataatta cccaccagag tgtgatatgc
     9901 gtacaaccat tgctgtagtg ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag
     9961 ataaaccaga cgttgccaaa tcggcaaacg atgaggtcag caccctgttt tttggtcatg
    10021 atgatcgtgt gccagtgaat gacacggccc aatcaccgtg ggatgcggtt gggcaactgg
    10081 aaacggccag cggcaattta tgtacggcga cgctgattgc acccaatctg gcattaacgg
    10141 caggacactg tttattgaca cctccaaagg gtaaagccga taaagcagtg gtgctgcgtt
    10201 ttgtgtcaaa taaaggtctt tggcgctatg agatccacga catagaaggc cgcgttgatc
    10261 cgacactggg aaagcggtta aaagcagatg gggatggttg gattgtacct cccgcagccg
    10321 cgccgtggga cttcggattg attgtgctac gtaatccccc ttctggcatt acaccgttgc
    10381 cgttatttga gggagataaa gccgcgctta ctgccgcatt aaaatcggct ggccgtaaag
    10441 taactcaggc gggataccct gaagatcatc tcgatacgtt gtacagtcat caaaactgtg
    10501 aagtgactgg ctgggcgcaa acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg
    10561 acagcggttc gcctctgatg ttgcatacca atgacggctg gcaattaatt ggggtgcaaa
    10621 gttcggctcc tgccgcgaaa gatcgctggc gcgccgataa ccgggccatt tctgttaccg
    10681 gttttcgcga caagctggat caactgtcgc aaaaataatg ttcaaacgct gcccgactgt
    10741 gcgggcagcg tgttcatcag gcaagtttca ccatgatcat tccagccaac agcaagacca
    10801 ggccaatcca gcctttacga tttaaccgtt gaccaaacaa gatccaacct gcggctaacg
    10861 tggcggcaat accaaacccg ccccacaatg cataagcgac agacaagtcg atccctttaa
    10921 cggcctgaga aagcgcacta aaggcagcca gcaccgccgc cagggagagc aagccaaata
    10981 ttttgcgacg aaagccgtca gaaaatttca aaaagacgtt agcaacgatt tccagcacga
    11041 ttgccaatgc cagccaggcg gcgtgaaccc attcaaactg cgccatggtt tacctccagt
    11101 tcaggtttac gcgctttacg ggtacctgat tttatcaaca caatcccggc gaccagggtg
    11161 gttaacccgg caattttcat cagcgataaa ctttcgtcga ataacaaaac gctaaacaag
    11221 gtaataaata aaataccgat accttcccac aacgcataag ctacgcctaa ggcgatcttt
    11281 ttaacggcga aagagagaaa tatatacgac agagaaatca tcaccagcat taaaataaag
    11341 ccgccatttc cctcactgac gctcgcccat ttcattgaca gcgtaccggt aatttctgta
    11401 gcaatagcca ggcctaataa aatccaataa atatacattg tccttctcct gcaagagaat
    11461 tattttaatt ttcgcttaat tcagcgaaat caaagtaaag tggtcaagcc ccgcgcatag
    11521 ggcaagctta ggcagaagaa aggactaaag cgcgttgcgc cagtgctgct cacctacgag
    11581 caagatagtg gatgaggtac aaagatgggt aaatgttgaa aataaggtcc tgaacaaatt
    11641 gtccataata ttacaattat ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc
    11701 tcggtagttg aacacgcctg atttgtatca tagcttaaga attaactcaa aatattttca
    11761 cttctttacc tgagcggttt gattttcgtt atgatgacgg agcgaaaaag acattattat
    11821 tagcaaagga agaaaaaacg gggacaagca tggcaaagcc gatcatcacg ctcaatggcc
    11881 taaaaatcgt cattatgttg ggaatgctgg tcattattct ctgcggtatc cgttttgccg
    11941 ccgagatcat cgtgccgttt attctcgcat tatttattgc tgttattctt aacccgctgg
    12001 tgcaacacat ggtccgctgg cgagtgccgc gtgtactggc ggtgtcgatt ttgatgacca
    12061 tcatcgtgat ggcgatggtg ttgctattag cttatctggg ttccgcgctc aacgagttga
    12121 cgcggacgtt accgcaatat cgcaactcta ttatgacgcc gctgcaagct cttgagccgt
    12181 tgttgcaacg cgtagggatt gacgtctcag ttgaccagct ggcgcattac attgatccga
    12241 acgcagcgat gacgttgctc accaacttat tgacgcagtt atctaatgcc atgtcatcaa
    12301 tatttttatt gctgctgacg gtgctgttta tgctgctcga agtgccacaa ttgcccggaa
    12361 aatttcagca aatgatggcg cgtccggttg aagggatggc ggcgattcaa cgtgcaattg
    12421 acagtgtttc tcattatctg gtgctgaaaa cagccatcag catcatcacc ggcctggtcg
    12481 cctgggcgat gctcgccgca ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct
    12541 ttgcgcttaa ttacatccct aatattggtt cagttctcgc ggcaatcccc cctatcgctc
    12601 aggtactggt gtttaatggc ttctacgaag cgttgctggt gctggcggga tatctgctga
    12661 ttaatctggt cttcggcaat attctggagc cgcgcatcat gggacgtggg ctggggcttt
    12721 ccacattggt ggtatttttg tcgttgattt tttggggatg gttgttagga ccggtgggta
    12781 tgctgctttc cgtgccgtta acaattattg tcaaaattgc gcttgaacaa acagcgggag
    12841 gtcaaagcat cgccgttctg ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc
    12901 cgtcagggtt acagagcttt caggattgca tccacgctgg ctttggcgtc accaaacagc
    12961 atgtgggtgt tttccttgaa gaacagcggg ttttgcacac cagcatagcc agtgttcatc
    13021 gaacgtttaa agacaatcac gtcctgcgct ttccacactt ccagcacagg cataccagca
    13081 ataggactct tcggatcatc ctgcgccgcc gggttaaccg tatcgttagc accaatcacc
    13141 agtacggtat cggtatcagc aaagtcatcg ttgatctcgt ccatttccag cacgatgtca
    13201 tacggtactt ttgcttcagc cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc
    13261 gggtggatac cgaaacgcac gttgatacca cgagcgcgca gtttctcggt aatttcagcg
    13321 acaggatatt gcgcctgcgc gactgccatg ccgtaccccg gagtaatgat cactgaatgg
    13381 gagtttttca gcagttccgc tgtctcttct gcggtgattt cgcggtgctc gcccacttcc
    13441 tgatcatcgc cagtagaaga gccgtcggtg ccgaaaccac ccgcaataac gctgataaag
    13501 gaacggttca tcgccttaca cataatgtaa gaaaggatag cccccgaaga accgaccagc
    13561 gcaccggtca caatcagcag gtcgttgctg agcataaagc ccgcagccgc agccgcccag
    13621 ccggagtacg agttcagcat cgacaccacc actggcatat ctgcaccacc gatggaggcg
    13681 actaaatgcc agccgaatac cagcgcaatg gcggtcatta ttagcaatgc cagcacttgc
    13741 aggccgacgc tgtccgtgcg aacaaataca atcagcagca ggaaggaaac gaccagagcc
    13801 gccaggttca ttttgtgacg gtttggcagc atcaacggtt tagacgaaat cttgccacac
    13861 agtttgccga acgccaccac cgaacccgtg aacgttaccg ccccgatgaa gataccgagg
    13921 aacacttccg tcaggtgaat attgaccaga atcggtgcca ttcccgcgtc atgatgcaga
    13981 tagctgttaa agccaaccag cactgccgcc agacccacga agctgtgcag gatcgccacc
    14041 agttctggca tttcggtcat ttcaactttc ttcgccagac ggataccaat tgccccacca
    14101 atgaccatcg ccagcaagat ccagccaaca ttacccgtat ccggtccaaa aatggttgcg
    14161 attaacgcaa tcgccatccc tgcgataccg aagttgttac cctggcgaga cgtttcatgt
    14221 ttcgaaagac cggccagact gaagataaac aggatcgcgg caacaatgta tgcagctgta
    14281 actaatcctc cagacatatg ttacccctta atttttgcgg aacattttca gcatgcgctg
    14341 agtcacggtg aagccaccga aaatattaat gctggctata agcaccgcga taaaactaag
    14401 gaagctaacc cagccgccct ggccaatctg caacagtgct ccgacaacaa taatccctga
    14461 aatcgcgttg gtgaccgaca tcaacggtgt atgcagcgcg tgcgatacat tccacaccac
    14521 gtaataaccg acaacgcagg ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg
    14581 cgcaacgctt gccagccaac cgaaaaggat gattgccagc gccatcaacg cgtatttacg
    14641 ccacggtgag caggcacatt tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc
    14701 ctgcggctga gctgatacct gaatcggcgg tgccgcctgc ggctgagctg atacctgaat
    14761 cggcggtgcc ggccaggtaa tttcgcccgc acggatcacg gtcacgcggc gaatcaccac
    14821 atcatcaaaa tcaacagtga tattgccgtc tttctctttg cacaacagtt tcagcagatt
    14881 aacgaggtta gtaccgtaaa gctgcgagga ttgcgtcggc agacggcccg gaagatcggt
    14941 ataaccaatc actttgacac cattttccgt agtgaagatt tcacccggca cggtgtattc
    15001 acagttgcca ccgttttggg ctgccaggtc gacaatcaca ctgcccgtct tcatggagtc
    15061 aaccatttca cgggtaatta gcttcggcgc tggtttgcct ggaataagcg cggtggtgac
    15121 aatgatatcg acctcttttg cctgggcggc aaagagttcc atttctgctt taatgaacgc
    15181 gtccgacatc actttggcat agccatcgcc gctgcccgct tcctctttaa aatccagctc
    15241 gaggaattcc gcgcccatac tttgaacttg ttctttcact tccggacggg tgtcgaatgc
    15301 acgcacaatc gcgccgagac tgtttgctgc gccaatggcg gccagacctg caacacccgc
    15361 accaatcacc atcacttttg ccggtggcac tttcccggcc gcagtaattt gcccggtaaa
    15421 gaagcgccca aattcatgtg ccgcttcaac aatggcgcga taaccggcga tgttcgccat
    15481 cgagcttagt gcgtccagcg attgtgcgcg tgagatacgc ggcacagaat ccatcgccat
    15541 cacggtcacg ttacgttccg caagtttttg cattaattcc ggattctgcg caggccagat
    15601 aaaactcacc agcgttgtcc caggattcag taacgcaatt tcatcatcta acggcgcatt
    15661 gaccttcaga atgatctctg actgccagac gctattccct tctacaattt cagcgcccgc
    15721 ttgcacaaac gctttatcgt caaaacttgc cagttgaccc gcgccgctct ctaccgcgac
    15781 ggtaaaaccc agtttcagca gctgttccac tgtttttggc gttgctgcaa cacgggtttc
    15841 attggttaac cgttctcttg gtatgccaat tcgcatgata ttcccttcca tcggttttat
    15901 tgatgatggt ttgcctgtgt caggagccac acaagccgct catgtacgag ctaaatgtta
    15961 ctccgttaaa ataaattagt aacaaacgcc ctataacgta ctgaaaatta tgcctgtgat
    16021 ctagcgccaa aaatcagtat ttcggcgtga actcgcaaaa tattaacgat tcagccgtga
    16081 tagtgggata aacaccttag aacgccggat aaagactgat aattgtcttc gacggtcggg
    16141 taaaacgaga catcgccccg gcacgattca ctacttaaca ttaaattaac ttatacaatt
    16201 cagttgcttc agtagtaatg atgctgatac ggctgttttt taagcataga cggtcatttg
    16261 agcaggatta aaattggctt aaggaatgtg atatgaaaaa tgacgcagac agttacaccg
    16321 tttaaatgca ataatcagcc acgtttctcg ttaataacaa taccagtacc tggtttgcgc
    16381 aaggcgaagg attattttta tgaagcttaa gaacaccctc ctggcgtcgg cactgctttc
    16441 tgctacggca ttctccgtta acgcagcaac agaactgaca ccggagcaag cggcagcggt
    16501 taaacctttt gaccgtgtag tggttaccgg tcgttttaat gctattggcg aagcggtgaa
    16561 agccgtttct cgtcgcgcag ataaagaagg tgccgcctct ttttatgttg tcgacacttc
    16621 tgattttggt aacagcggta actggcgtgt ggtcgctgac ctctataaag ccgatgctga
    16681 aaaagcagaa gaaacaagta atcgcgtaat taacggtgtt gtcgaactgc cgaaagatca
    16741 ggctgttctg attgaaccgt ttgacacggt caccgtccag ggcttctatc gtagccagcc
    16801 agaagtcaat gatgccatca ccaaagcggc aaaagcgaaa ggtgcctact ctttctacat
    16861 cgttcgtcaa atcgatgcca accagggcgg caaccagcgt attactgcat tcatctataa
    16921 aaaagatgct aagaaacgta tcgtccagag cccggatgtg atcccggcag attccgaagc
    16981 aggacgtgca gctctggctg ccggtggcga agccgcgaag aaagttgaga tcccaggtgt
    17041 tgcgactacc gcatcaccaa gttctgaagt cggtcgtttc tttgaaaccc agtcatcaaa
    17101 aggcgggcgt tacaccgtca cgctcccgga tggcactaaa gtcgaagaac tgaacaaagc
    17161 gaccgcagcg atgatggtcc cgttcgatag catcaaattc tctggcaact acggcaacat
    17221 gaccgaagtc tcctatcagg ttgcgaaacg tgccgcgaag aaaggtgcta agtattacca
    17281 catcacccgc cagtggcagg aacgtggtaa taacctgacc gtcagcgcag atctgtataa
    17341 ataactattc agttactcct ataaggcggc ttgatgccgc ctttcgcatt tttagtcaca
    17401 aatttccccg cgaccattgc atgcagacac atctcttcgt aaaatcccgc gcttcagtgg
    17461 caaactccat ttttatgcgt tttgccctaa tatttattct tcttatcacg ttttaatcac
    17521 tggatatcga tggaaaagaa actgggactg agcgcactca ccgcgctggt attaagctca
    17581 atgctgggcg cgggtgtttt cagtctgccg caaaatatgg cggcagttgc cagcccggca
    17641 gcactgctca tcggctgggg tattactggc gctggcattt tattgctggc ctttgccatg
    17701 ctgatcctca cgcgcattcg tcccgaactt gacggcggta tctttaccta tgcccgcgaa
    17761 gggtttggcg agctaatcgg cttttgttcc gcatggggat actggctgtg cgcagtcatc
    17821 gccaacgtct cctatctggt gatcgttttt tccgcgttaa gcttttttac ggacacgccg
    17881 gaattgcgcc tgttcggcga tggtaatacc tggcaatcga tcgtcggtgc atcggcttta
    17941 ttgtggtttg ttcacttttt gatcctgcgc ggggtgcaaa ccgctgccag cattaacctg
    18001 gtggcgacac tggcaaaatt gttgccgctg ggtctgtttg ttgtgctggc gatgatgatg
    18061 ttcaaactgg ataccttcaa gctcgacttc accggacttg tacttggcgt acccgtttgg
    18121 gaacaggtga aaaacaccat gctgatcacc ctgtgggttt tcattggtgt ggaaggtgcc
    18181 gtggttgttt ctgcgcgtgc gcgtaataaa cgtgatgttg gcaaagcgac actgctggcg
    18241 gttctctccg ctctgggcgt ttacttgctg gtaacgctgc tttcgctggg tgtggtggca
    18301 cgtcctgaac tggctgaaat tcgtaacccg tcaatggccg gtctgatggt ggaaatgatg
    18361 ggaccatggg gcgaaatcat catcgctgcc agtttgatcg tttccgtttg cggcgcgtac
    18421 ttaagctgga ccatcatggc ggcagaagtt ccgttcctgg ctgccactca taaagcattc
    18481 ccacgcattt ttgcgcgcca gaacgcacaa gctgcgccat ccgcctcact gtggttgacc
    18541 aacatctgcg tgcaaatttg tctggtactc atctggctga ccggttccga ttacaacacg
    18601 ttgctgacca tcgcctcaga aatgattctg gtgccctatt tcctggtcgg cgcgttcttg
    18661 ctgaaaatcg caacccgtcc actgcataaa gcggtaggtg tcggtgcctg catttatggc
    18721 ttatggttat tgtacgcctc tggtccaatg cacttgctgt tatccgttgt tctgtatgcg
    18781 ccggggctgc tggttttcct gtatgcgcgc aaaacgcata cacatgataa tgtactgaac
    18841 cgtcaggaaa tggtgctgat tggcatgctg ttaattgcct cagttccggc gacctggatg
    18901 ctggtgggat aacgttcatc ccatcgttgg atagcaaagg agataacgat gggtaaagcc
    18961 cagcccttgc caatattaat tactggcgga ggtcgtcgca tcggcctcgc cctcgcctgg
    19021 catttcatta atcaaaagca accggtgatt gtcagctatc ggacgcacta tccagccatt
    19081 gatggactga ttaaggcagg tgcgcagtgt attcaggctg atttttcgac caacgacggt
    19141 gtgatggcgt ttgccgatga agtactaaaa agcacccatg gtctgcgtgc tattttgcat
    19201 aacgccagtg cgtggatggc ggaaaaactg ggtgcgccac tggccgacgt actggcttgc
    19261 atgatgcaga tccacgttaa taccccatac ctgctcaacc atgcgctgga aagattactg
    19321 cgtgggcacg gacacgccgc cagcgatatc attcacttta ccgattatgt ggtggagcgc
    19381 ggtagcgaca agcatattgc gtatgctgca agcaaagcgg cactggataa tatgacccgc
    19441 tcgtttgccc gcaagctggc accggaagtg aaagtgaatt ctattgctcc atcgctgatc
    19501 ctgtttaatg aacatgatga tgccgaatat cgacaacagg cgctgaataa atcactgatg
    19561 aaaaccgcgc ctggcgagaa agaagtgatc gacctggtcg attacttact taccagttgc
    19621 tttgtcaccg gacgcagttt cccacttgat ggcggtcgtc atctgcgtta atgcagcttt
    19681 atccagcata taatcaacac ccatgcaaca cgcaacagaa ccaacaaaag cggcgggcaa
    19741 gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg gaataatcga
    19801 ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc tggcaacaat
    19861 ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc agcccggcga tataataatt
    19921 tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg cggctttgat
    19981 tttaccttaa cacttccata acaagtcatc agtagaatac ctgatgaaaa cttgtttaga
    20041 aacgattgat agtaagtaaa aacagcgcgg tgtattgtga cgtttttata tctaccgtga
    20101 atgttatgaa cactatcgta tttgtggaag atgatgcgga agtcggttca ctgattgccg
    20161 cgtacctggc aaaacatgat atgcaggtta ccgtagagcc gcgcggcgac caggccgaag
    20221 aaaccatttt gcgagaaaat ccggatttgg tgttactcga catcatgcta ccaggcaagg
    20281 acggcattac catttgtcgt gatttacgcg caaagtggtc tggaccgatt gttcttctaa
    20341 cctctctcga tagcgatatg aaccacatcc tggcactgga aatgggtgcc tgcgactata
    20401 ttctcaaaac gatgccccct gctgttttgc tagcgcgttt acgtttgcat ttgcgtcaga
    20461 atgagcaagc cacactgacc aaaggtcttc aggaaacgtc tctgactccc tacaaagccc
    20521 tgcatttcgg cacgttgacc atcgatccca tcaaccgcgt agtcaccctg gctaacactg
    20581 aaatctcgct ctcgacagct gatttcgaat tattgtggga attagctacc catgccgggc
    20641 aaatcatgga ccgcgatgca ttgctgaaaa atttacgcgg cgtcagttat gacggactgg
    20701 atcgtaacgt ggacgtggct atttcgcggt taagaaaaaa actgctcgat aacgccgcag
    20761 aaccttatcg cattaaaact gtgcgtaaca aaggctatct ttttgcgcct catgcatggg
    20821 aataagcgat gaaaaaactg tttatccagt tttacctgtt attgtttgtc tgcttccttg
    20881 tgatgtctct gctggttggg ttggtgtaca aatttaccgc cgaacgcgcg ggcaaacagt
    20941 cgctggatga tttgatgaac agttcgctgt atctgatgcg cagcgaattg cgtgagatcc
    21001 ccccacacga ctggggtaag acgctgaaag agatggattt aaatctctct ttcgatctgc
    21061 gtgtcgagcc actgagtaaa taccatcttg atgatatttc catgcaccga ctgcgtggcg
    21121 gcgaaattgt cgccctggac gatcagtaca cgtttttgca gcgtatcccg cgcagccact
    21181 acgtgctggc agttggtcct gttccttatc tttattatct ccatcagatg cgattgctgg
    21241 atatcgccct gatcgctttt attgctattt ccctcgcctt tccggtgttt atctggatgc
    21301 gtccgcactg gcaggatatg ttaaaactgg aagcagcggc gcaacgattt ggcgatgggc
    21361 atctctatga gcgtatccac tttgatgagg gttcgagctt tgaacgactt ggtatcgcat
    21421 ttaaccagat ggcggacaat atcaacgcct tgattgccag caaaaaacag cttattgacg
    21481 gtatcgctca cgaactgcga acaccgttag tgcgcctgcg ttatcgactg gagatgagcg
    21541 ataacctgag cgccgccgag tcccaggcgt tgaatcgtga tatcagtcaa cttgaagctt
    21601 taattgaaga gctgctgact tatgcccgac tcgatcgccc acaaaacgag cttcatctta
    21661 gcgaaccaga cctgccgttg tggctgtcaa cgcatctggc agatattcag gcagtaacgc
    21721 ccgataaaac ggtacggata aaaacgctcg tgcaaggcca ttatgcggcg ttggatatgc
    21781 gcttaatgga gcgcgtgctg gataatttgc tcaataacgc cctgcggtac tgccattcaa
    21841 cggttgaaac cagcctgcta ctgtcgggga atagagcgac attaattgtt gaggatgatg
    21901 gcccagggat tgccccagaa aaccgcgaac atatctttga accttttgtt cgcctcgatc
    21961 ccagccggga tcgctcaacc ggcggctgcg ggctggggct ggcaattgtc cactctatag
    22021 cactggcaat gggcggtacg gttaattgtg acaccagcga actgggtggt gctcgcttct
    22081 cgtttagctg gccgttatgg cataacatcc cgcaatttac ctctgcctga cactacgcgc
    22141 acgatggtca agtcaccacc attgtgctat aaattaagga tgttgtaact aaagtggtta
    22201 atattatggc gcgttacgat ctcgtagacc gactcaacac tacctttcgc cagatggaac
    22261 aagagctggc tgcatttgcc gctcatcttg agcaacacaa gctattggtt gcccgcgtgt
    22321 tctctttgcc ggaggtaaaa aaagaggatg agcataatcc gcttaatcgt attgaggtga
    22381 aacaacatct cggcaacgac gcgcagtcgc tggcgttgcg tcatttccgc catttattta
    22441 ttcaacaaca gtccgaaaat cgcagcagca aagccgctgt ccgtctgcct ggcgtgttgt
    22501 gttaccaggt cgataacctt tcgcaagcag cgttggtcag tcatattcag cacatcaata
    22561 aactcaagac cacgttcgag catatcgtca cggttgagtc agaactcccc accgcggcac
    22621 gttttgaatg ggtgcatcgt catttgccgg ggctgatcac ccttaatgct taccgctcgc
    22681 tcaccgttct gcacgacccc gccactttac gttttggctg ggctaataaa catatcatta
    22741 aaaatttgca ttgtgatgaa gtcctggcac agctggaaaa aagcctgaaa tcaccacgca
    22801 gtgtcgcacc gtggacgcgc gaggagtggc aaagaaaact ggagcgagag tatcaggata
    22861 tcgctgccct gccacagaac gcgaagttaa aaatcaaacg tccggtgaag gtgcagccga
    22921 ttgcccgcgt ctggtacaaa ggagatcaaa aacaagtgca acacgcctgc cctacaccgc
    22981 tgattgcact gattaatcgg gataatggtg cgggcgtgcc ggacgttggt gagttgttaa
    23041 attacgatgc cgacaatgtg cagcaccgtt ataaacctca ggcgcagccg cttcgtttga
    23101 tcattccacg gctgcacctg tatgttgcag attaacgccc ggctttcata ctgccgacca
    23161 tctgttctgg ccgtacccag ctgtcaaact cggcttcgct aagatacccc agcgcaaggg
    23221 ccgcagcttt taaggtcagc ccttctttat gcgctttttt ggcgatctcc gcggctttgt
    23281 cataaccaat gtgggtgtta agcgcagtca ccagcatcag cgattcattg agtaattgat
    23341 tgattcgctc acgattcggt tcaataccca ctgcgcagtg tttgttaaaa ctttccatgc
    23401 catctgccag caagcgcacc gattgcagga aattatggat caccatcgga cggaagacgt
    23461 tcagttcaaa gttaccggaa gcgccaccca tgttgatcgc cacgtcgttc cccattacct
    23521 gacagcagag catggttaat gcttcacact gggttgggtt cactttcccc ggcatgattg
    23581 agctgcccgg ctcattttcc gggattgaga tttcaccaat tccgcagcgc gggccagagg
    23641 ccagccagcg gacatcattg gcgattttca tcagtgacgc agccaaccct ttcaacgcgc
    23701 cgtgcgcctg aaccagggca tcacaggtcg caagcgcttc aaatttgttc ggcgcggtaa
    23761 caaacggtgc acaggtaatg actgccagtt catctgctac gcgacgcgca tactccggat
    23821 gggtatttag tccagtaccc accgctgtac cgccaagagc cagttccgct acgtgaggca
    23881 ggctgtattc gatatgtttg agattatgct cgagcatcgc tacccagccg gaaatctcct
    23941 gccctagtgt tagcggcgtg gcgtcctgca agtgggttcg accgattttg acgatatcgg
    24001 caaatgcacg cgatttttca ctcagtgtct gcgtcagggt tttaagctgc ggaatgagtt
    24061 gcttgcgcag cgccagcagc gccgcaacgt gcatcgccgt cggaaagaca tcgttggaac
    24121 tttggctttt gttcacgtcg tcgttagggt gaactttacg ttccatcccg cgcacgccgc
    24181 cgagtaattc actggcccgg ttagccagca cttcgttcat gttcatgtta ctttgcgtgc
    24241 cggagccggt ctgccagata gccagcggga attcgtcgtc atgctgtcct gccagtactt
    24301 catccgccgc ctgccgaatg gcgctcgctt tctcttcaga caacaagcct aaatcttcat
    24361 taacttttgc cgctgcgcgc ttggttagcg ccagcgcatg aatcagtgag gtgggcattt
    24421 tctccgtcga aatgcggaaa tgctccagcg agcgttgagt ttgtgcgccc cacagcttat
    24481 ctgccgggac atcaatcgcc cccatcgaat ctttttcgct gcgtactgta ttcatggcct
    24541 gctcctcacc tgtttaattt tttctttctg ttttgctttc gctaagcaac ttttagccca
    24601 cttattattt actatttgat aacaagtgtt tggtctttcg tgccatgtaa aaaaaccg
//
LOCUS       DAJVOY010000047        24538 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVOY010000047 DAJVOY010000000
VERSION     DAJVOY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.047"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(395..1276)
                     /gene="yddG"
                     /locus_tag="OQL90_001684"
     CDS             complement(395..1276)
                     /gene="yddG"
                     /locus_tag="OQL90_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1368499.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1508..4555
                     /gene="fdnG"
                     /locus_tag="OQL90_001685"
     CDS             1508..4555
                     /gene="fdnG"
                     /locus_tag="OQL90_001685"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2093..2095,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1368500.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4568..5452
                     /gene="fdnH"
                     /locus_tag="OQL90_001686"
     CDS             4568..5452
                     /gene="fdnH"
                     /locus_tag="OQL90_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1368501.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEAPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5445..6098
                     /gene="fdnI"
                     /locus_tag="OQL90_001687"
     CDS             5445..6098
                     /gene="fdnI"
                     /locus_tag="OQL90_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1368502.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(6149..6433)
                     /locus_tag="OQL90_001688"
     CDS             complement(6149..6433)
                     /locus_tag="OQL90_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1368503.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(6579..7589)
                     /gene="adhP"
                     /locus_tag="OQL90_001689"
                     /pseudo
     CDS             complement(6579..7589)
                     /gene="adhP"
                     /locus_tag="OQL90_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7723..9420)
                     /gene="maeA"
                     /locus_tag="OQL90_001690"
     CDS             complement(7723..9420)
                     /gene="maeA"
                     /locus_tag="OQL90_001690"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1368504.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9577..9714)
                     /gene="sra"
                     /locus_tag="OQL90_001691"
     CDS             complement(9577..9714)
                     /gene="sra"
                     /locus_tag="OQL90_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCS1368505.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(9816..10031)
                     /gene="bdm"
                     /locus_tag="OQL90_001692"
     CDS             complement(9816..10031)
                     /gene="bdm"
                     /locus_tag="OQL90_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCS1368506.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10377..10808
                     /gene="osmC"
                     /locus_tag="OQL90_001693"
     CDS             10377..10808
                     /gene="osmC"
                     /locus_tag="OQL90_001693"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1368507.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(10864..11790)
                     /locus_tag="OQL90_001694"
     CDS             complement(10864..11790)
                     /locus_tag="OQL90_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1368508.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11783..12769)
                     /locus_tag="OQL90_001695"
     CDS             complement(11783..12769)
                     /locus_tag="OQL90_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1368509.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12766..13662)
                     /locus_tag="OQL90_001696"
     CDS             complement(12766..13662)
                     /locus_tag="OQL90_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1368510.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPRAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13659..14681)
                     /locus_tag="OQL90_001697"
     CDS             complement(13659..14681)
                     /locus_tag="OQL90_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1368511.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(14683..16233)
                     /locus_tag="OQL90_001698"
     CDS             complement(14683..16233)
                     /locus_tag="OQL90_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1368512.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(16247..16828)
                     /gene="ddpX"
                     /locus_tag="OQL90_001699"
     CDS             complement(16247..16828)
                     /gene="ddpX"
                     /locus_tag="OQL90_001699"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCS1368513.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(17086..19485)
                     /gene="dosP"
                     /locus_tag="OQL90_001700"
                     /gene_synonym="pdeO"
     CDS             complement(17086..19485)
                     /gene="dosP"
                     /locus_tag="OQL90_001700"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCS1368514.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTDLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(19510..20891)
                     /gene="dosC"
                     /locus_tag="OQL90_001701"
                     /pseudo
     CDS             complement(19510..20891)
                     /gene="dosC"
                     /locus_tag="OQL90_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(21263..22582)
                     /locus_tag="OQL90_001702"
     CDS             complement(21263..22582)
                     /locus_tag="OQL90_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1368515.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22713..24248)
                     /gene="gadC"
                     /locus_tag="OQL90_001703"
     CDS             complement(22713..24248)
                     /gene="gadC"
                     /locus_tag="OQL90_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1368516.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         6125 a         6183 c         6189 g         6041 t
ORIGIN      
        1 tagtaaaata acgtagagcg gtaaccttgc cataaaggtc gagtttatta ccatccttgt
       61 tatagacttc ggcagcgtgt gctacgttcg cagctaacaa accagttaca accaccgcaa
      121 ctatttttaa tttcatttaa cagaatcctt ttaattatcg tcaaacgtat tttctaaacg
      181 aattttaaac ggcgtcattt ataaatgaca tactgttttt aaaatagaat ggtgattaaa
      241 aacaatgaga aaaatagatg agataatatt atttatcgat atgtgatcga agtcgaaatg
      301 agatataagg tgaattactg gtatttgaaa tttatttttt aatattgtcg gaatttatct
      361 gattaactgc cgggccgtag acccggcaat tattttaacc acgacgtgtc gccagccagc
      421 agagcaggga accgccgcag accattagcg cgccttgcca gaatgagaac gacagcgggg
      481 cgctgagcag cacggctgca agcgctgagg aaagtacggg cgtaaaatac gagcctaccg
      541 ccattatggt gacattgcca tgcaatatac cgacattcca tgcagcataa gcaaatccta
      601 aggtaaatgc cgcagagatg agtttaatca tgacgggcgt gctaaatacc atttctggtt
      661 gttgcgtaag aaaatagtaa acccacagac ttgctcccgt tagcaggaca aaaacggtaa
      721 ttccgttaaa tccgcgtgcg tatttattcg ttactgtgca ataggctgcc cagataaacg
      781 caccaataaa cgccaggaaa taactcaatg ggctggtggt gatattattg atgatttcat
      841 cataatgtaa cccattctca ccgcctaaca cccagcagac gccgaccagg gctaataata
      901 atcctggtac aatcagccag ttggttttct gaccattaaa cagaatggca aagagaattg
      961 tcaggctggg ccacagatag ttcaccatac ccgcttcaat cgcctgatga cgggtcgccg
     1021 cataccctaa ggaaagcgcc agacagattt catagctgac gaataacaga ctcccggcga
     1081 gtaaatagcc tttcggaatc tgccgaatac gcggaaatcc aaccgtgaag attaacagca
     1141 gcccgcttaa tgaatagata gcagctgcgc cgccgaccgg gccgagcccc tcgctgacac
     1201 cgcgaatcaa tcctaccatc gtgctccaca ggacgatcgc tattagccct atgagcgttg
     1261 ctttttgtcg tgtcatgctc gctgttttgt ctctcttgcc gttaaaaatt aagctgaatt
     1321 ttatagcatt tttttaactg gcctgtcagg cagtggtgcg tttttctacc gctattgagg
     1381 taggtcaatt tgcgcaggcg gattattttg tggcaaacac gtgttctttt tgatttcgcg
     1441 caaaaagatt cagaatttta ctgttagttt cctcgcgcag taatatccct gaaaaaagag
     1501 gaaagcaatg gacgtcagtc gcagacaatt ttttaaaatc tgcgcgggcg gtatggctgg
     1561 aacaacggta gcggcattgg gctttgcccc taagcaagca ctggctcagg cgcgaaacta
     1621 caaattatta cgcgctaaag agatccgtaa cacctgcaca tactgttccg taggttgcgg
     1681 gctattgatg tatagcctgg gtgatggcgc gaaaaacgcc agagaagcga tttatcacat
     1741 tgaaggtgac ccggatcatc cggtaagccg tggtgcgctg tgcccaaaag gggccggttt
     1801 gctggattac gtcaacagcg aaaaccgtct gcgctacccg gaatatcgtg cgccaggttc
     1861 tgacaaatgg cagcgcatta gctgggaaga agcattctcc cgtattgcaa agctgatgaa
     1921 agctgaccgt gacgctaact ttattgaaaa gaacgagcag ggcgtaacgg taaaccgttg
     1981 gctttctacc ggtatgctgt gtgcctccgg tgccagcaac gaaaccggga tgctgacaca
     2041 gaaatttgcc cgctccctcg ggatgctggc ggtagacaac caggcgcgcg tctgacacgg
     2101 accaacggta gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca accactgggt
     2161 ggatatcaaa aacgctaacg tcgtaatggt aatgggcggt aacgctgctg aagcgcatcc
     2221 cgtcggtttc cgctgggcga tggaagcgaa aaacaacaac gatgcaacct tgatcgctgt
     2281 cgatcctcgt tttacgcgta ccgcttctgt ggcggatatt tacgcaccta ttcgttccgg
     2341 tacggacatt acgttcctgt ctggcgtttt gcgctacctg atcgaaaaca acaaaatcaa
     2401 cgccgaatac gttaaacatt acaccaacgc cagcctgctg gtgcgtgatg attttgcttt
     2461 cgaagacggc ctgttcagcg gctacgacgc tgaaaaacgc cagtatgata agtcgtcctg
     2521 gaactatcag ttcgatgaaa acggctatgc aaaacgcgat gaaacactga ctcatccgcg
     2581 ctgtgtgtgg aacctgctga aagcgcacgt ttcccgctac acgccggacg tcgttgaaaa
     2641 catctgcggt acgccaaaag ccgacttcct gaaagtgtgt gaagtgctgg cctccaccag
     2701 cgcaccggat cgcacaacca ccttcctgta cgcgctgggc tggacgcagc acaccgtggg
     2761 tgcgcagaac atccgtacta tggcgatgat ccagttgctg ctcggtaaca tgggtatggc
     2821 cggtggcggc gtgaacgcat tgcgtggtca ctccaacatt caggggctga ctgacttagg
     2881 tctgctctct accagcctgc caggttatct gacgcttccg tcagaaaaac aggttgattt
     2941 gcagtcgtat ctggaagcga acacgccgaa agcgacgctg gctggtcagg tgaactactg
     3001 gagcaactat ccgaagttct tcgttagcct gatgaaatct ttctatggcg atgccgcgca
     3061 gaaagagaac aactggggct atgactggct gccgaagtgg gaccagacct acgacgtcat
     3121 caagtatttc aacatgatgg acgaaggcaa agtcaccggt tatttctgcc agggctttaa
     3181 cccggttgcg tccttcccgg acaaaaacaa agtggtgagc tgcctgagca agctgaagta
     3241 catggtggtt atcgatccgc tggtgactga aacctctacc ttctggcaga accacggcga
     3301 gtcgaacgat gtcgatccgg cgtctattca gactgaagta ttccgtctgc cttcgacctg
     3361 ctttgccgaa gaagatggtt ctatcgctaa ctccggtcgc tggttgcagt ggcactggaa
     3421 aggtcaggac gcgccgggcg aagcgcgtaa cgacggcgaa attctggcgg gtatctacca
     3481 tcatctgcgc gagctgtacc aggccgaagg tggtaaaggc gtagaaccgc tgatgaagat
     3541 gagctggaac tacaagcagc cgcacgaacc gcaatctgac gaagtggcta aagagaacaa
     3601 cggctacgcg ctggaagatc tctatgacgc taatggcgtg cttattgcga agaaaggtca
     3661 gttgctgagt agctttgcgc atctgcgtga tgacggtaca accgcatctt cttgctggat
     3721 ctacaccggt agctggacag agcagggcaa ccagatggct aaccgcgata actccgaccc
     3781 gtccggtctg gggaatacgc tgggatgggc ctgggcgtgg ccgctcaacc gtcgcgtgct
     3841 gtacaaccgt gcttcggcgg atatcaacgg taaaccgtgg gatccgaaac ggatgctgat
     3901 ccagtggaac ggcagcaagt ggacgggtaa cgatattcct gacttcggca atgccgcacc
     3961 gggtacgcca accgggccgt ttatcatgca gccggaaggg atgggacgcc tgtttgccat
     4021 caacaaaatg gcggaaggtc cgttcccgga acactacgag ccgattgaaa cgccgctggg
     4081 cactaacccg ctgcatccga acgtggtgtc taacccggtt gttcgtctgt atgaacaaga
     4141 cgcgctgcgg atgggtaaaa aagagcagtt cccgtatgtg ggtacgacct atcgtctgac
     4201 cgagcacttc cacacctgga ccaagcacgc attgctcaac gcaattgctc agccggaaca
     4261 gtttgtggaa atcagcgaaa cgctggcggc ggcgaaaggc attaataatg gcgagcgtgt
     4321 cactgtctcc agcaagcgtg gctttatccg cgcggtggct gtggtaacgc gtcgtctgaa
     4381 accgctgaat gtaaatggtc agcaggttga aacggtgggt attccaatcc actggggctt
     4441 tgagggtgtc gcgcgtaaag gttatatcgc taacactctg acgccgaatg tcggtgatgc
     4501 aaactcgcaa acgccggaat ataaagcgtt cttagtcaac atcgagaagg cgtaaggggg
     4561 cgaacagatg gctatggaaa cgcaggacat tatcaaaagg tccgcaacta actccatcac
     4621 gccgccttct caggtgcgtg attacaaagc agaagtcgca aaacttatcg acgtttccac
     4681 ctgtatcggc tgtaaagcct gtcaggtggc gtgttcggag tggaacgaca tccgtgatga
     4741 agtggggcac tgcgtcgggg tttacgataa ccccgccgat ctgagcgcca agtcctggac
     4801 ggtgatgcgc tttagcgaaa ccgaacagaa cggcaagctg gagtggctga tccgtaaaga
     4861 cggctgtatg cactgtgaag ctcccggctg cctgaaggcg tgcccgtctg ctggtgcaat
     4921 cattcagtac gctaacggga ttgtcgattt ccagtcggaa aactgcatcg gctgtggtta
     4981 ctgcattgcc gggtgtccgt ttaatattcc gcgcctcaac aaagaggata accgggtata
     5041 taaatgcacg ctctgcgtcg atcgcgtcag cgtcggccag gaaccggctt gtgtgaaaac
     5101 ctgtccgacc ggggctatcc acttcggcac caagaaggag atgctggagc tggcggaaca
     5161 gcgcgtggcg aaactgaaag cgcgtggtta cgaacatgct ggcgtctaca acccggaagg
     5221 ggtcggtggt acgcacgtta tgtacgtgct gcatcacgcc gatcagccgg agctgtatca
     5281 cggtctgccg aaagatccga agatcgacac ctcggtaagc ctgtggaaag gcgcgttgaa
     5341 accgctggca gcggctggct ttattgccac ttttgccggg ttgattttcc actacatcgg
     5401 tattggcccg aataaggaag tggacgatga cgaggaggat catcatgagt aagtcgaaaa
     5461 tgattgtgcg caccaaattt attgatcgcg tctgtcactg gaccgtggtg atttgcttct
     5521 tcctggtggc gctgtccggg atttcgttct tcttcccgac gctgcaatgg ctgacgcaaa
     5581 ccttcggtac gccgcagatg ggacgcattt tgcacccgtt cttcggcatt gcgattttcg
     5641 tcgcgctgat gtttatgttt gtgcgttttg tgcatcacaa catcccggat aagaaagata
     5701 ttccgtggct attgaacatt gtcgaagtat taaaaggcaa tgagcacaaa gtggcggatg
     5761 ttggtaagta caacgccggg caaaagatga tgttctggtc gatcatgagc atgattttcg
     5821 tgctgttggt gaccggggtg attatctggc gtccgtactt tgcgcagtac ttcccgatgc
     5881 aggttgttcg ctacagcctg ctgatccacg cggctgccgg tatcatcctg atccacgcca
     5941 tcctgatcca tatgtatatg gcattctggg tgaaaggatc gattaaaggg atgatcgaag
     6001 ggaaggtaag ccgtcgctgg gcgaagaaac accatccgcg ctggtatcgt gaaatcgaga
     6061 aggcagaagc gaaaaaagag agtgaagaag ggatataatc tcttttgaac tttaagctga
     6121 aaatggcgct ttaagaagcg ccattttttt attgcgtaac cagacggcgc agtcgcgaca
     6181 catccaccgt tttttcagcc tctgccagac tccaggcgtt ttgcagattc agccacattt
     6241 gcggcgaact gccgatcacc acggaaagtt taattgccat ttctggcgtc aaagctgctt
     6301 ttccggtcag caatcggctt gccgttgagg gcgcaatttc cattgctctg gcaaactcgc
     6361 gcaggctgac attaagttcg tccagtgatt cctgaataat gtcccccggg cggggatgat
     6421 tggccatttt cattagtgtc tttactctca gtagcgttcc ttctctggga acactatcat
     6481 tattgttcct tcctacgcaa caataatcca tttagccatt tcatgttgga gagtgtgcga
     6541 tgctgcgacc cgaacatggg agtcgcagca aagacctctt agtgacggaa atcaatcacc
     6601 atacggccac ggattttgcc ttcttccatc tcggtaaaga tggtgttgat gtccgctaac
     6661 ggacgcaggg cgactttcgg caccactttg ccttcggcgg caaactggaa ggcttcggtt
     6721 agatcctggc gcgtgccgac cagcgaaccg accacttcaa tgccatccag cacaagacgt
     6781 gggatatcca ggctcataga ctccggcggc agaccgacag ccacaacacg accgcctgca
     6841 cggacagcat caactgccga gttaaacgca gctttagcta ctgctgttac caccgcagcg
     6901 tgagcgccac cggttttctc ctgcacaatt taggcggcgt cttcggtgcg tgagttaatc
     6961 gctaaatctg cacccatttc ggttgccagt tttaactgct catcattgac atcaatggcg
     7021 atcactttgg cgttaaagac attcttcgcg tattgcaggg cgaggttacc cagaccgcca
     7081 agaccgtaga tagcaatcca ctgaccggga cgaatttttg acagtttaac ggctttgtag
     7141 gtggtgaccc ccgcacaggt aatgctgctg gccgccgccg agtccagacc atctggcact
     7201 tttaccgcgt aatcggcgac cacgatgcac tcttccgcca tcccgccatc aacgctgtat
     7261 ccggcatttt taactgaacg gcagagcgtt tcgttaccac tgttacagta ttcgcaatga
     7321 ccgcatcctt cgtaaaacca cgccacgctg gcacgatcgc ctggttttaa tgaggtgaca
     7381 cctgggccaa cttctgccac cacaccgatg ccttcatggc ccagaattac cccagttttg
     7441 tcaccaaaat cgccattctt aacatgaaga tcggtatgac atacaccaca acactccatt
     7501 ttcagcaggg cttcgccatg tttcagtgag cgcagtgttt tatccgtaac gtcaacatga
     7561 tgatccttcg taacaactgc agccttcata gttcctcctt ttcggatgat gttctgcata
     7621 gcaggtgagg caaatgagat ttattcgcca ctacccagta tggatgagat ctgaaaaagg
     7681 gagagggaaa tagcccggta gccttcacta ccgggcgcag gcttagatgg aggtacggcg
     7741 gtagtcgcgg tattcggctt gccagaaatt atcgtcaatg gcttgttgca aagcttcagc
     7801 agacgttttc accgccacgc cttgctgctg cgccattttg ccaaccgcaa acgcaattgc
     7861 gcgggagact ttctgaatat ctttcagttc cggtagtacc agaccttcgc cgttcagcac
     7921 cagcggcgaa tactgagcaa gcgtttcact tgctgacatc agcatctcat cggtgatacg
     7981 tgacgcgccg gaagcaataa cacccagccc gatgccaggg aaaataaagg cgttgttaca
     8041 ctgggcgata gggtagattt tatctttcca taccactgga ttaaacgggc tgccagtggc
     8101 gaccagcgcg ttaccttcgg tccaggcgat aatgtcctgc ggtgtggctt ccacgcgtga
     8161 cgtcgggtta gacagcggca tcacgatcgg acgcggacag tgtttatgca tctcacggat
     8221 gatctcttcc gtaaacagcc cggtctgtcc tgagacgcca atcagaatat ctggttttac
     8281 attgcgcacc acatccagca gtgacagcac atcgctgtcg gtatcccagt cactgaggtt
     8341 ttcgcgtttc tgcaccagtt tggtctggaa aggcagcagg ttcggcatct tgtccgtcag
     8401 caggccaaag cgatcgacca taaagacttt ctgccgcgcc gcttcctcgc ttaatccttc
     8461 gcgctgggtc tgggcgatga tcatttcggc aatgccgcat cccgctgaac ctgcaccaag
     8521 gaagacgatt tttttctcgc ttaactgacc acctgccgct cggcttgctg cgatcagtgt
     8581 gccgactgtt accgccgcag tgccctgaat gtcatcgtta aaagaacaaa tttcattgcg
     8641 atagcggtta agtaacggca tcgcattttt ttgagcaaag tcttcaaact gcaacagcac
     8701 gtctggccag cgttgtttca cagcctggat aaattcatca acgaattcat agtattcgtc
     8761 gtcaatgata cgcggattac gccagcccat atacagcgga tcgttaagca gctgttggtt
     8821 gttcgttccg acatccagca ccaccggaag ggtatacgcc gggctgatgc cgccacaggc
     8881 ggtatagagc gacagtttac cgatcggaat gcccatcccg ccgatgccct ggtcaccaag
     8941 ccccagaata cgttcaccgt cagtcaccac aatcactttg atattatggt tcggtacgtt
     9001 ttgcagaata tcgtccatat tgtggcggtt ctggtaagag ataaacacgc cgcgtgaacg
     9061 gcggtagatc tcagaaaaac gctcacaggc tgcgccgacg gttggggtat aaataacagg
     9121 catcatctca tcaagatgat tgtttaccag acggtagaag agggtttcgt tggtgtcctg
     9181 gatgttacgc aggtagatgt gtttgtcgat ttcggttttg aatccctgat actggatcca
     9241 tgctcgttcc gcttgttctt cgatggtttc gaccacttcc ggcagtaacc ccagcaggtt
     9301 gaagttacgg cgttcttcca tgctgaaggc actgccttta ttcaacaacg gaaattccag
     9361 cagtacaggg ccagcgtaag ggatataaag cgaacgctgt ttttttgttt ttggttccat
     9421 gtcactcact cttttttgaa tatccatccc tggcacgctt ttatcgtctt tgctttaccg
     9481 ccagggcgtc ggccccaagt ataaagcaga taaaaacaaa aacaccattg cgcaggcaat
     9541 ggtgtttaat cgtcattgag gactgatggt tatgaattac ttttcagcgg ggcgttttct
     9601 gccggttggg ttatttacca cgctggattt gtcaccttcg gtaactattt tgcgctggtt
     9661 agaaatttta tggtccagtc caagaatatg acgtgcctga cggttcgatt tcattttaac
     9721 tcctcaatcc tgttgctagt tttaaggaca acatcgccgt agcgaagaaa cacgtgctaa
     9781 acccctaaat taggttgccg atcaagcata gcaccttaaa gcgtagggtg ctggccactg
     9841 accacataat tgatcgtttg ctggtagata tcactgagga tgtcgttatc agaagcttcc
     9901 acccatttgg tcagctccat gagaatgtca tcttcagtga caacaccgtg ctctgcccga
     9961 aggccttgct caatggcatt aaccagagcg ggttctgctg ttgaattttc tgcctgataa
    10021 taagtaaaca tagtgattct ccgtgtctgt gtatttatgg tgtctgctac ggatcgcaga
    10081 tttataaagc acattcagca tggcaaatat ttgccgcttt gttgttaaga ttagtcctgg
    10141 ttgatgattt ttatatttta acctcatgat attgataggt attattattg tggtgagttg
    10201 attaatatta atgcaatatc tcttgggtta tatattcccg gttatctatt gtggaaattt
    10261 aatttaagtg cagaagtaat atttctgccg gatttttatt cggaatatcc tgcttatcct
    10321 cgtgctgttt ctcacgtagt ctataatttc ctttttaagc ccacatgaga gcaacaatga
    10381 caatccataa gaaaggtcag gcacactggg aaggcgatat caaacgcggg aagggaacag
    10441 tatccaccga gagtggcgtg ctgaaccaac agccgtatgg atttaacacg cgttttgaag
    10501 gcgaaaaagg aactaaccct gaagaactga ttggcgcagc gcatgccgca tgtttctcaa
    10561 tggcgctttc attcatgctg ggggaagcgg gatttacgcc aacatcgatt gataccatcg
    10621 ccgatgtgtc gctggataaa gtggatgccg gttttgcgat tacgaaaatc gcactgaaga
    10681 gtgaagttgc ggtgccgggt attgatgcct ctacctttga cggcatcatc cagaaagcaa
    10741 aagcaggatg cccggtctct caggtactga aagcggaaat tacgctggat taccagttga
    10801 aatcgtaaag cattgccgga tgacgtgtca ggcgcgtgaa tgcctgatgc gttgttagca
    10861 tctctacagc gcccgccagc accggagctc acgtccgtcc tcccttattg ctaatgattg
    10921 ccggacttca catccgctgg ttgcaagggg gcaacgttcg tagaaaaaac agccttgcgg
    10981 caacgtgcgg tttcccggca gatccgtttt acgtaatgcc cattcttcct ccagcggttt
    11041 atcaatggcg gggagggaat ccagcaataa tcgggtgtat ggatgtgccg gagccgttag
    11101 cacctgttgc gcgtcgccca gttctacaat ctgcccgaga tacatcaccg ccacccgatc
    11161 gctcatatga cgtatcaccg agacattgtg tgaaatcagc acataggtca gcccgtgatt
    11221 ttcctgtagc gttaccagta aattgaggat ctgcgcctgc acggagatat ccagcgcaga
    11281 ggttggctca tcaagcacaa tcacgtcagg ctgcgaagag agtgctctgg caatggcgat
    11341 acgttgccgc tgcccgccgg agaacgcatg aggcaggcgg tcgagatact ccggacgaat
    11401 acccacctgc acagccagct cctctaccaa cgctcgccgc tgttgttcac tactacgctt
    11461 agctatccag agcggttctg tgatgatgcg ccacaccggt aagcgcggat tgagcgaaga
    11521 gagcgggtcc tgaaacacca tctgcataat gcgttgtgag ccagaacgga tgtactgccc
    11581 gtggctcggt tgcagcatac ccattaaaag ctgtgcgagg gtgcttttgc cgcagcctga
    11641 ctcgccgacg atccctaagg tttcaccacg gcggatctgt aaatcaatac cattaatcgc
    11701 atgaacatgt tccgtagttt taccaagcca gtttttacgg gccgggaaat tgatatggac
    11761 gccgcgtaac gttaataacg tgtcagacac ttatgacctc ctgctgcgga taccaacagg
    11821 cgcagcgctg gttgttgtca ccacacgctg tcagcgccgg gacgttttca cactgtgcac
    11881 ctgccgcata gcaacgatcg cgaaaagcgc agccgtcagg caaatgggtg aggtttggta
    11941 ccgtccctgg aatggcgggt aatagctggc gtggtactcc atgttctggt gcacattgga
    12001 gcaaaccaat ggtatacgga tgccggggat gatggataac gtctgccctc acgccgcttt
    12061 caattacgct tccggcatac atcacgtaaa cgctatcgca cagttgcgac accacggcca
    12121 tatcatggct gatgaacaga accgcagttc cactggcgcg ggctttatgt ttaagcaaac
    12181 gcagtacctg caactgcacc gtgacgtcca gcgccgtagt cggttcgtcg gcaataatca
    12241 attgcggctc gcaggagaat gccagcgcaa tcattacccg ctggcgcata ccacctgaaa
    12301 gctcaaacgg atagcgcgac ataacttcca cggcatccgg gatttgcatc tcttccagca
    12361 ggacaatcgc tttagctctg gcttcccgac gacttattgg ttgatgatgg cggatcacgt
    12421 ccatcatctg aagacctatt cgacgtgtcg gattgagggc ggtcatcggt tcctgaaaga
    12481 tcatcgccac tcgtgcgccg cgccactgac gaagctgttt ttcccgagcg ttaagaacat
    12541 cgtctcccag cagtgaaatc tgcccccgat gtacgcaata actgcccgtc ggtagcagac
    12601 gcataatcag cattgcggtg actgatttac ctgagccgga ttctcccacc agaccgacaa
    12661 tttcaccgcg gttaatctgc aaggacacat tgttgagcgc gtgaacatca ccgttaaaac
    12721 cggggaaact caaatgcagc tgttgaatgt ccagaacggg ctgggtcatg actgctttcc
    12781 tcctgctttc gggtcaagca gatcgcgaat accatcacca aagagattaa acccaacggc
    12841 ggtaagcaaa atcgctgccc ttggaaatgc gcaataccac cactgatcga gtacatagtt
    12901 gcgaccattc gctaccatcg cgccccattc agcactcggt tgttgagcac ccaggccaat
    12961 aaatcccaac gtggcggcca ttaaaatcgc gctaccgata tccagcgatg cctgcacgat
    13021 cagcggcggc agggagttac gtaaaatatg ccagctgatc agatgccaac gcgacgcacc
    13081 aaaggttttc gccgcctgaa cataggtata ctggcgtaca actaatgctt gcccccgcgc
    13141 caggcgcaca taaaagggaa ttcgcacaat agcaatcgcc agcatggcgt taaataaact
    13201 cggcccgaga gcggcagcca gtgccattgt cagtaccaac gaaggaatcg acagcataat
    13261 gtccatgatg cgcatgatga tggcgtctgc gcgtccgcca agcacaccgg atagacatcc
    13321 gagtagcgaa ccaatcatac ccgcaatggc aaccacgact aatccggcga gaattgattg
    13381 ctgactgccg accagtacgc ggctgaacag atcgcgtccc acttcatcgg tgccaaacca
    13441 gtgcgccgca gaaggcggca aaaggcgggc ggttaaatca atggcgttgg gatcatgcgt
    13501 cgcaagccac ggtgaaaaaa acatcatcaa tagcattaat acaatgatga ctgcgccggt
    13561 cacggtgagc ggactgcctt tcagcatcca taccagtttt gcaccgttaa atcgcgtttg
    13621 tttttgtggg cgtacggcgg acgtttcctc gcttagcatc attcaccacc tccacgtccg
    13681 atacgcggat caatccacaa atagagcaaa tccaccacca ggttgaccag cacataagca
    13741 aatgaaacca cgacggcaaa gcccatcact gccgggaagt cgagcgcctg tattgatgtt
    13801 actacccatg cacccattcc gggccaggca aaaacggttt cggtgagcac tgcgccatac
    13861 aacaaatcgc ccagcgccag acccaatacg gtaatcgatg ggatcaacgc attgggtagc
    13921 gcataacata aaacgatata ccagccgggc aagccgctgg cccgggcggt acgaatgtag
    13981 tcttcactca attgttccag catcgctgag cggatctggc gagcgacaat tcccaggtga
    14041 acgaacgcca gcgttaatgc cggtaagatg agatgttgca acgcattgaa gaagacttca
    14101 ccgttgcctt caagcagcgc atcgagcaga taaaagccgg taacgtgcgt tggtggatcc
    14161 agccagtcat caagccttcc gccgccggga agaatttgca gatgaccata aaacagcaca
    14221 atgacgccca gtccaagcca aaacgcaggt gtggagattc cggtaatggc cattattcgc
    14281 accagatgat ccagccagcg atttcgccag actgcagaga gtatgcccag cgggatgcca
    14341 atcaggagtg ccagcagcag ggcacaaaaa gccagttcca gcgtcgccgg gaaaaatata
    14401 cgcaactctt ccaacaccgg acgcccggta cgaatggatg ttcccaggtc accatgaaac
    14461 aggtcgctga cgtagcggta aaactgtaca tacagtggct ggtccagtcc cagttgctgg
    14521 cgaatatttt ccacgatagc atcgctggcg cggtcacccg ccagtaaccg cgccggatcg
    14581 ccagggatca gatgcgagat aataaacgta atcacgcaga cgcccgccat cacgagcacc
    14641 agcccccagc agcgttggcg taaaatactc cagaaggtca cgttatttac tcatggtatt
    14701 gatattgaag acctgttcca gcatgggatt gaacacaaag cctttcacct ctttgttcat
    14761 cgccagttgg tagtttttct ggaacaggta cacataagca gcgtcatcaa tgacgatttt
    14821 ctgtgcctgc tggtagtccc gggtacgctg cgtctggtcg gtggtcgcaa gcgcattgcg
    14881 cagtaactta tcgacctcac tgttttcata gaacgagcgg ttacccggca gacctttttt
    14941 gtctgactca aaccagtaat tcataaacat atacgggtcg gcaaaatccg gactccagtt
    15001 gccaatcgca atgtcgtaat cacctttacc cactctgtcg cgcatggtgg cgttcgccag
    15061 cttttccagc ttcacattga tgcccagctt gttgagactg gattgtgtcg ccagagcaat
    15121 aggctcccag ttcggatcct tatcggagta gagaaacgtc aggctggtgg gtttgctcgt
    15181 cactttatcc cattcggctt tggctttcgt ttcgtcatgg ttgtattgca ttgccgtcgc
    15241 atcgtagccc cacatgcctt ccggaatcgg gccgcgcatc tgttttccgt taccactcag
    15301 aatgccgtta accatgccct gataatcggt agaccaggaa atggcccgac gcagatccgc
    15361 ctgattaaga ggcgctttgc tgttattcag atacaggtag gtgacgcgca gtgacggata
    15421 ctctgccaca ttgactttgt tttcctgctt cagggcgttg agttgatcca ccggcagcgc
    15481 atcggcaatg tcaatatcac cacgggagag ctgcaggcga cgggaggcac tttcaccaat
    15541 aatttttacc gatactcgct taaagttcgg tttattgcca gggtaatgcg gatttggcac
    15601 cagaactaat tgctgacctt tttgccagct tttcagcata aatggtccgg aaccggcggt
    15661 attttgcgcg aggaagccgc gggcatcatc cgctgcatgt tcctttaaca ccgccggatt
    15721 gataatggat gcaccgtcat tcgccagcgt gtagaggaac ggtgcgaatg gctggctaag
    15781 ggtaaacttc accgtatgtt cgtcgggagc atcaatcttt aaatctttgg gaaatgcttc
    15841 tgctggcccc tggccgattt ttagtagccg ctcaaaagag agttttactg cttcggcagt
    15901 gaccggtgtg ccatcggcaa atttagcatt gtccttcagg gtgaacgtcc actctttttg
    15961 atcgtcagac gctttccagc tacttgccag atcgccttca acgtcggttg agcctttatc
    16021 accgtccgtt ttgtactgaa ccagccgctg ataagacggg taggtcaccg tccagtcgtt
    16081 attatctatt gttaccgccg gggcgagggt ttgtggatcg gcggccttac caatcaccag
    16141 catatctttc ggtacggcgg cgtgcgcaac cgggaaagtt gtggcaagga cgagcgcgag
    16201 caatgtagga cgaaacggta tcgatctctt catgacagtt tcctgattaa ctgacgtgct
    16261 gtgtgccagg ggatataaaa caggtgaatt gatcagcgag cagtgggtaa ctcgctgcct
    16321 ggggtaattc gaagtgccac cattcgctgg agataccgac aaaaccgcca ccagtcataa
    16381 tcgcattcag caacagccga ttgcgctgag cggcgggcgg gacggaaggg tgataggcat
    16441 gggagcgctc gtgcatttca tcgaaccctg cgcccatatc gaggatgttc ccgtgctcat
    16501 cacgaagcgt caggtcgatc gccgtgccac ggctgtgatt agaaccgacc gtcacatcaa
    16561 caacatattg cgggtctggg caggcttgcc acaacatcgc ctgtgcttgt tgtgggcgat
    16621 acgcatcgta aatcaccagt tgtaagcctg acagctgggc gatactgata cttttcgcca
    16681 gcgcggtaat cgcatccttg tgtagcagac aacgcgcttg ctgataaata gctttacctg
    16741 tgatgttatc agcgcaggcg tatttcaatt cgatctccag atcagggaag attacggcta
    16801 aatcaaccag ttcggtggta tccgacataa acggttcctg tataagacaa aaattgctgc
    16861 gctttccgct tatgcagatc tcatgccatg ccgggataag cgccagaatg ctggcttaaa
    16921 gttatatttt ttaacttttg atcaacattt gtgcagcgta gtgcagtttt ggtgcaagag
    16981 gggaagttaa ggaaggaatc tcccggaatc gtagatgaaa tcacagtatt taagtgacgg
    17041 tgtcacgtta aatgaaaacc cgcgagtgcg ggcgagagga atttgtcaga ttttcagcgg
    17101 taacacgctg ctcatccagc ctggaatttc ttcggcgggt agggggcggg aaaagaaata
    17161 tccctgaata acgcgacagt ggatcttgcg tagcatctca aattgctctt tggtttcgac
    17221 gccttccgcc acgacggtta aattgaggct ttgcccaatg ctggtaatgg cttcaagtaa
    17281 ggcaaggatg cgtttttcgg tcagacaacg atcgacaaaa cttttgtcaa ttttgatttc
    17341 cgttaccgga agactgacta agcgggataa tccggaaaag cccgtaccaa aatcatctac
    17401 cgataagccc acgcccatat cacgcaggat ctgaatgcgc ttaaagattt cggtatcgtg
    17461 ttccatcatc atgctttccg tgatttctac cgtcagctgg tggccgtcaa taccccaggc
    17521 gtgcattgca tcagacacct gattaggcag ttgattactg cgaaagtgca gtgccgacaa
    17581 gttaacggaa agcgccggga tatgaatatt ctggctacgc cattctgcta actgacggca
    17641 agcttccgcg atgacccagc gcccaatatt ttcgatttca ccaatctctt ctgcgagagg
    17701 aataaaccgt gaagggggca catgaccatg ctggggatcg tgccagcgcg caagggcttc
    17761 gatgccgtac agttcacccg tttctgcgaa gatttgcggc tggtaaacaa gtttcagttg
    17821 gttattgcta atcgcttctt tcagcgctgc gcctaaaacc aaacgctctt ttaccatttc
    17881 gttcatcgcc gggctgaaga actgccagcc gttaccgcca ttcttgcgaa tataatctat
    17941 tgcattgtga gcagtggaga gtaggtaatc gtggttttta cccacgtcgt agctgatgcc
    18001 aatactcaag gtaagcggga atggtttatc gtcaatcatt atcggcttgc tgaccacatt
    18061 ccgtagctca tcggcgatct gggtaatgtt actgacgtcg ttttccaggc tcacgaggac
    18121 aaactgcgta ccttcgatac gacagagata ctgatccggt ttgagttttc cacgaaagcg
    18181 attgaccact tccagcaatg cctgatcggc ccacgcatag ccaaggctat caatcacatc
    18241 ctgaatatgg tcaacaccga tgagatacac caccggagag acggctttgt cgaccaggtc
    18301 atcgaggtaa ttgtgcaggt tattgcgatt tggcagatcg gtcatcggat caaattggat
    18361 gagttgttca atatgctggc ggcttttttc ctgttccagc gccagcgcgg ccatatgctg
    18421 actgatatct gccacgcgtt cgataaaggc gctggtttct gctcctgacg aggttttaat
    18481 ttgcaggatc cccgcaggcg cgccatcacg ctgacgaatg gtcgctgacc agctttgcgc
    18541 attttgaact tctgcaccgt gcgaagatga cgcccagtgt atcggcatcc cgttgcgcag
    18601 tgcgaacagc gaaacatgcg attcgttgag aacagattcg atgttacgac aaatgatttc
    18661 ccccatttca tgaaatggcg ggctgctgca catggcggcg agaatattgc cttcaagctg
    18721 gcggatttgc cgttcttcgg tgatatccga gaaagtcatt accaggttct gcagatgcgc
    18781 gagcacgtca taaaccgggc tgatagaggc tttaatccag attttttcac cggtgcgcgt
    18841 caacagcaga aattcgtcct gatcgcgggc ggttttccat agcaactgtt gtaaacgaat
    18901 gcggttatcg gcagggaatt caggaatgtt caggagtgta tcgggctgca taccgctggc
    18961 ttcgctaatg cagtaaccaa acatttcggt aaatgcgcga ttgcactgca caatatggcg
    19021 ttccggatcg aggacaatca ccggtcggtc gagatggtca acggcaataa tcaattgtcg
    19081 ggtctgttct ttttgcgcca tttctacgct ggcatcccgt accagcgcca ggtaataaac
    19141 tttcccctcg gcgctcactt tcgatagcgc aaaacgggtc cagattttac tgccgtcttt
    19201 tttctccagc tgcagctccc gactcatccc ctcgacgcgc gctttaccac cttcacggtt
    19261 atgacgaatg tattcaggat gcgcaggacg caaatcccgc ggaatcagca tatcaatgtt
    19321 attgccaatg acttcttcac gtttgtaccc ccagagcttc tctgcggcgg ggttgaaaaa
    19381 catcacttca tcattttcgt taattaacac cgcgcccatc atattttgtt caagggcggg
    19441 gaaaaaaatg ccatcggcgg cattttccgc atcggttagc ttcatgatta cctctgcatc
    19501 ctggcgcatc taaagactgg ctttccagag ttcaacacgg tttctacctc gtcttttggc
    19561 gatatacaga gcttcatcgg ctatttgaat gaggcgctca tagtcaggat gaccattaaa
    19621 catggcggca ccgatggaaa gtgagagggc aatatcctcg ccgtttgcgg ctttcagttt
    19681 ggttttctcc acccgactgc gaatacgttc tgcggtacgt aacgtttcgt tttcagaagc
    19741 ttcagtcaaa acaatgataa attcatcgcc cccgtagcga aaaacataat cactactgcg
    19801 gacgttgtca taaaaggcct gagagacttt acgcagaatt tcatcgccag tgttatggcc
    19861 ccacgtatcg ttgatctctt tgaatttatc aacgtcaata atcagcactg acagcggtgt
    19921 accggtccgg ttggcatggg caatttcgcg tttgaagata gtcggcagga agcggcggtt
    19981 aagtaatttc gtcagtacat ccataccgac ttcgtggcgc gatacttctt caaacaattc
    20041 acgcagcaag gtaataattt gcgatacagt atttcttatc tgtaataaaa atttcacccg
    20101 cagacttctg ttattcaaat tcctggtgtt acgcatggtt tgattgaata taccgtcgaa
    20161 atcctgaatc agacgggaga tatgcctact tcggcaatgc cactaaaata atgtcgacct
    20221 ttatggttaa accacaggcc aaaatcagcc tggcttaaag gcaaactact gcctaaatca
    20281 gaatccagca ggattttata gataatatct atttcccatg aaagtattga ggctatttgc
    20341 cgttcttttt cttcttcggc gttttctagt aacgagaaga tacgatagtt ttcatcttcc
    20401 tttgaggcac tactgtcact aaaggtaaac gcgcgggtca tcacttccat agcgatatca
    20461 atactgttaa tcgagaaatg gtagacctga agtttttctg cggcagaata atccgaagag
    20521 aagattaccg gatagaggat ttttttcagc acccgaaacc ccatctcgac aatttctacc
    20581 ggaattccta tgcgggcatg cacttccgcg acggtatgct ggatttgtat tagcctttcg
    20641 acatcgtcaa cctgggcaga aagcacgtta ataatccagc gttccatcgc acttttcaac
    20701 tgccgctcaa cttgttcatt actcaagaat tcttcggcat gcgggtcgat gcggacaatt
    20761 cgataaaact cgatactcag ataatgagca tgcgcaacgg caatttccgc ggctttagca
    20821 cgaatgagtg gatctgcccg ttcgacaagt ccggtccact catctttcat tcttttaaaa
    20881 tacatctcca taattcacac ccttataagg ctgggaaatc agacggaatc aaaacgcaac
    20941 gtgcgagatc gactaactgc accatattct cctgaaatat gaagatatac tgaaaagaaa
    21001 taagcgactt aggacagttt caatctacgc tactgttctt cagaagagta tagcccatcg
    21061 taattatttt tcggtgacag cgaatatcgt atggtttttc atattcatac atttttatta
    21121 gggatttata gctgtttaac taagtgtggt taattgcgtg gttaatttga cttatgtaag
    21181 catgattact agtgggatat ttcacaaggt gtaacaagcc ggtgggtaaa gcaccggctt
    21241 gttacaaagt aagcatggga gtttaactgc cccaacgact ttgcagatag ctgaccgctt
    21301 gttgagtctg cggtttattc agatagtcct cacggaacaa gatggtgccg ctaatttctg
    21361 gtaccgcatc gttaagatcg agctgctttt tcagttccgg tacgccgccg ttaatcatcc
    21421 agtctggctc tatctttgaa ggttcaccca ctttatagaa ggcgataccg atatacaggc
    21481 gggtcctggt cggtttaacg acatccgccc accattttgc caacacgtca taacgcgcgg
    21541 cactccgtga gaacggccag taaatttggg gagcaatgta atccagcaat ccttgttcca
    21601 cccatcgacg ggtgtcagcg taggattcgt cataggctgc cgcgcctcgg gtatcggaac
    21661 cgagcggatc gtgtgatcgg ttacgccaca cgcctgccgg gctaacgcca aattcgactc
    21721 ccggcttaat gcttttaatg gtgtgcgata cttttgtaat taactgctga gtattgttgc
    21781 gccgccagtc tgcttttgac gcaaatgcgc ctccgtattt acggtacgtt tcgttatcat
    21841 ttagccgtga acccggtgac tccgtataga aatagtcgtc aaactgcacg ccatctaccg
    21901 gatagcggga aaccacttcg gcgactattg atgtgatcca gtcctgaacc tcagggatgc
    21961 ccgggtcgag gacaaagcga tcgccagacg ttctgatcca gtcacggtgt tgcacataga
    22021 cgctcgccgg ttgttgagac agagtgctat tcagttccct gatagtaccg ggcttcgtat
    22081 taaccgatac gcgatagggg ttaaaccagg cgtgtacttt catcccacgc ttgtgggctt
    22141 cgtcgagcat aaattgcagc ggatcgtaac ccggattttc accaatctta ccggtcataa
    22201 gatcggacca cggcaaaatt tttgatggcc acagggcggt accgtccggc ttgacctgga
    22261 aaaagaccgt gtttataccg agacgttgca gatgatccag tttgtcgatc atcgcctgtt
    22321 gttgtacacg ggcccggctg gtggggttac taatgttaac cgaggaaacc ggtggccagt
    22381 cgaggcgaga caccgtggcc agccagatgc cacgcatcgg ttgtgacgat tgttgcgtcg
    22441 tggctggtgg ctttgaacct gctggtggtg tcaccatgga ctctggaggc gtgcttttac
    22501 aactacacag taaaagtgcc agtgcaacca gtatcgctgg tcttctaatc gttaatttct
    22561 tgtttcggga gcagatatcc atatgtgctg gtttccggta aacagatgtg cgctcattct
    22621 cgtacttatc ccggttaagt caatacgaca gcaagcacga aaaagggagc gatgaattat
    22681 cgctcccttg tcttataacc attcagacat ggttagtgtt tcttgtcatt catcacaata
    22741 tagtgtggtg aacgtgcacg cgggtgcagg aagaagtgac cttttggtgc gttctgactg
    22801 ttgattggct ccagagtgac gccggtattc gctttgcctt tacgatcatg aacagcatag
    22861 agaataaagg gcagggcaag taccaccagg aaactaacga ccagtaattc aacatacata
    22921 tcggtagagt caccctggat gttatccggc ggcaggaagg aaacaataaa cgccataatt
    22981 gaagtcagca gaccgacaat tgccacgacc agttttaccc ctttaccacc agggatatta
    23041 aatgtgcgtt ttaagtcagg atgtttaaga actaacacaa tgtagccaat aaacagcatg
    23101 aaataagcac acagataaat caccaccgtc agcgccagtg cgatcaggaa ggacatgttg
    23161 ttaccgccac cggtattggt gaggatgatc aacgcgatag aagtaatcac cagctgcgaa
    23221 atgaccagcg ttaccggtac gccatttttg ttcattttag cgaatgccgc tggcagcagg
    23281 tttttctgcg ctgtgacata catcccgcga gaaggaccaa caatccagga ggcgatttcc
    23341 gccagaacac ccagcaacag cagtgcggag atcacgcgaa ccgtccactc aatttccggt
    23401 gccacatggg acatcagaac ggtaaaggtt tgcattaccc ctgcggagag gttgatttca
    23461 ttacccggaa tgaccatcgc aatagataaa ccgccaacag agcttaagca gattgccgcc
    23521 accatcagca gtaacatagc cagtggatag tcgcgcccgg ggttgctcat ttcattgacg
    23581 tgagttgcgg aagcttctac gcccatataa ctcaaaatga aggcaacaaa tacaaccagt
    23641 gtgcccactt tagagaagtc agggaagaag gtcttcgaat ccatttcgat agcaacgggg
    23701 gcaccggagt gcagataaat agccgctaat gcgatcaaaa taaatgcagg taacaggata
    23761 ccggcgaaga agccaacttt agcaattcgc gccgtgtatt tcgtgccacc aaactgcgtt
    23821 aatgccagcg cccaaagaat gatgagtgct gcaatagttt tggtaatggg gtcttcattc
    23881 agcgctggcc atttcaggat gtaggagagt gcccctaaca cgaaatagag catcggaata
    23941 aaaccaatgg cgatttgcag atagccaaat gagatcgctg caaatcccca tctcggcccc
    24001 agagtatttg atacccaggc gaagacacca ccttcttccc agccgtcgac ggtggccatt
    24061 tccgcagcac aaagtcccac gggaataaac cataaaatcc cgcctaatag caggaagaag
    24121 actaatgaaa agcccgatgt tgcgaaggta gggtattcat aaacagccat taccatcgat
    24181 gccgttatgg caaagaatcc gagtaatgtg agctgcttag ctttacctgt ctgtactgat
    24241 gtagccatat tatcccccta aaacggtatt cctgtcggaa ccgcacctgt tttgttctga
    24301 agcgtattca gaacaatatt ttccgttgct aatgccagtg aacagacttt ggaaattgtc
    24361 ccgaaacggg ttcgtttcgg gacaccgtta ccgttaaacg ttatcaggta tgtttaaagc
    24421 tgttctgctg ggcaataccc tgcagtttcg ggtgatcgct gagatatttc agggaggctt
    24481 tgtagtcttc cagcaacagt tcagcaaagt ccatttcgaa gccgcgacga cacataat
//
LOCUS       DAJVOY010000048        24534 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVOY010000048 DAJVOY010000000
VERSION     DAJVOY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.048"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(134..2989)
                     /gene="valS"
                     /locus_tag="OQL90_001704"
     CDS             complement(134..2989)
                     /gene="valS"
                     /locus_tag="OQL90_001704"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1368517.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVSL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(2989..3432)
                     /gene="holC"
                     /locus_tag="OQL90_001705"
     CDS             complement(2989..3432)
                     /gene="holC"
                     /locus_tag="OQL90_001705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1368518.1"
                     /translation="MKNATFYLLDNDTTVDGLSTVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3592..5103)
                     /gene="pepA"
                     /locus_tag="OQL90_001706"
     CDS             complement(3592..5103)
                     /gene="pepA"
                     /locus_tag="OQL90_001706"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1368519.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5370..6470
                     /gene="lptF"
                     /locus_tag="OQL90_001707"
     CDS             5370..6470
                     /gene="lptF"
                     /locus_tag="OQL90_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1368520.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6470..7552
                     /gene="lptG"
                     /locus_tag="OQL90_001708"
     CDS             6470..7552
                     /gene="lptG"
                     /locus_tag="OQL90_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1368521.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7713..9215)
                     /locus_tag="OQL90_001709"
     CDS             complement(7713..9215)
                     /locus_tag="OQL90_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1368522.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9345..10364)
                     /gene="ahr"
                     /locus_tag="OQL90_001710"
     CDS             complement(9345..10364)
                     /gene="ahr"
                     /locus_tag="OQL90_001710"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1368523.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            10560..10644
                     /locus_tag="OQL90_001711"
     tRNA            10560..10644
                     /locus_tag="OQL90_001711"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10594..10596,aa:Leu,seq:caa)
     gene            10808..12073
                     /locus_tag="OQL90_001712"
     CDS             10808..12073
                     /locus_tag="OQL90_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1368524.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(12128..13138)
                     /locus_tag="OQL90_001713"
     CDS             complement(12128..13138)
                     /locus_tag="OQL90_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368525.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(13089..13625)
                     /locus_tag="OQL90_001714"
     CDS             complement(13089..13625)
                     /locus_tag="OQL90_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368526.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            complement(13626..14705)
                     /locus_tag="OQL90_001715"
     CDS             complement(13626..14705)
                     /locus_tag="OQL90_001715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368527.1"
                     /translation="MNVGHLNFFKVNKCGLYKVNDDNTYGLELSETFDLIQDWVGTKS
                     LALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWKSDTDSTGSLLGASEDGEIGS
                     SSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFDHSVCDSELFQDYVHSSITNR
                     VKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLRSLETTHTELGKLIPKITHIV
                     RRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQIEIKAEAKPSLNEVQEIIEKY
                     SAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSDDYSTYSAAYIYEQISHKRKE
                     FISPILKELKALQSQPRIKKASGED"
     gene            complement(15103..15675)
                     /locus_tag="OQL90_001716"
     CDS             complement(15103..15675)
                     /locus_tag="OQL90_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HCS1368528.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAT
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(15749..16249)
                     /locus_tag="OQL90_001717"
     CDS             complement(15749..16249)
                     /locus_tag="OQL90_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCS1368529.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(16246..16980)
                     /locus_tag="OQL90_001718"
     CDS             complement(16246..16980)
                     /locus_tag="OQL90_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HCS1368530.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            17532..17798
                     /locus_tag="OQL90_001719"
     CDS             17532..17798
                     /locus_tag="OQL90_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1368531.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            17795..18394
                     /locus_tag="OQL90_001720"
     CDS             17795..18394
                     /locus_tag="OQL90_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1368532.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            18387..18674
                     /locus_tag="OQL90_001721"
     CDS             18387..18674
                     /locus_tag="OQL90_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HCS1368533.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            18667..19122
                     /locus_tag="OQL90_001722"
     CDS             18667..19122
                     /locus_tag="OQL90_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368534.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYQDIYPQDTVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            19227..19580
                     /locus_tag="OQL90_001723"
     CDS             19227..19580
                     /locus_tag="OQL90_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HCS1368535.1"
                     /translation="MQSSVTGRKANENTLLPPVLRAALYRRAVACAWLTVCERQHRYP
                     HLTLESLEAAIAAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVV
                     MDELCARHIKAPVLH"
     gene            19595..21928
                     /locus_tag="OQL90_001724"
     CDS             19595..21928
                     /locus_tag="OQL90_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCS1368536.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDVAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEVTRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPLDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            22094..22288
                     /locus_tag="OQL90_001725"
     CDS             22094..22288
                     /locus_tag="OQL90_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1368537.1"
                     /translation="MWIKDALVWIRATDPNLLIKNYLTFRFIDGLKIDWSEKQWGHKR
                     GHISLLCFIICFLSLTYFDI"
     gene            22506..23774
                     /locus_tag="OQL90_001726"
     CDS             22506..23774
                     /locus_tag="OQL90_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1368538.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            24165..>24534
                     /locus_tag="OQL90_001727"
     CDS             24165..>24534
                     /locus_tag="OQL90_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368539.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQSP
                     LKLSISKYIENAHKNIIDNDE"
BASE COUNT         5823 a         6362 c         6099 g         6250 t
ORIGIN      
        1 aataaagaag aacaaccaga taaaggcttc ttagccagta tttcacgggg aattatgcct
       61 tctttaccat ttctgtaaga gataaaaaag gccggagcat gctccggcct tcgttttcat
      121 cactgtgttt tgattacagc gcggcgataa cagcctgctg ttcaatcagc ttcgctttcg
      181 cttctgcata gccttccagc ttctcacgct ctttcgcgat gaccgcttcc ggtgcgcggg
      241 cgacaaagcc ttcattcgcc agcttgttct cgatacggct gatttcgcct tcgatcttcg
      301 ccacttcttt cgccagacgc gccagctcat cttctttgtt gatgaggcca gccatcggga
      361 tcagcagctc tgcgccgtcg atgatcttcg taacggaaac cggacctttg tcatcggcag
      421 gcagcacggt gatgctttcc agacgcgcca gggtttgcag gaagccacgg ttttcattta
      481 cgcgacgttc tgcatccgcg ctgcaaccac gcagcagcag ctccagcggt ttgcccggcg
      541 cgatgttcat ttctgcacgg atgttacgta ccgcaacgat cgcctgtttc agccactcgg
      601 tgtcagccag tgcggcttca tcaacctgag atgcatcgta ctgcgggaac ggctgcagca
      661 tgatggtgtc ggcagtgata ccgcaaagta ctttcacacg ctgccagatg gtttcggtga
      721 tgaacggaat gatcggatgc gcgaggcgca gcagaccttc cagtacagtc accagcgtat
      781 ggcgagtacc gcgcagttcg gcttcggttc caccgttcat taccggctta gtcagctcca
      841 gataccagtc gcagaactgg ttccaggtga actcatacag aatgcctgca gcgatatcga
      901 agcggaagct gtccagcgct tcgcggtacg ctttgatggt ctggttgaac tctgccagaa
      961 tccagcggtc cgccagcgac agcgtcattt caccaccgtt gaagccgcaa tcctgacctt
     1021 ccgtgttcat cagcacaaag cggctggcgt tccacagctt gttacagaag ttacggtagc
     1081 cttccaggcg tttcatatcc cagttgatgt cacgaccggt agaagccagc gccgccaggg
     1141 tgaagcgcag ggcatcggtg ccgtgcggct caatgccgtt cgggaactgt ttctcggtgc
     1201 gcttacggat tttgtccgcc agctgcggct gcatcatatt gccggtacgt ttctccagca
     1261 gttccggcag cgaaataccg tcaaccatat ccagcgggtc aataacgtta cccttggatt
     1321 tggacatctt ctggccttcg tcgtcacgaa tcaggccggt catgtaaacg gtgtggaacg
     1381 gcacctgcgg tttgccattt tcatctttga tgaagtgcat ggtcatcatg atcatgcggg
     1441 caatccagaa gaaaatgatg tcgaaaccag ataccatcac gctggttggg tggaactggc
     1501 gcagtgcgtc ggtgttttcc ggccagccta gggtagagaa ggtccacagc gcagaagaga
     1561 accaggtgtc gagaacgtct tcgtcctgac gcagggcaac gtcggcaccg aggttatttt
     1621 ccttgcgcac ttcttcttcg ttgcggccaa cataaacgtt acctgcttcg tcgtaccatg
     1681 cagggatacg gtgaccccac cacagctgac gagagataca ccagtcctga atatcgcgca
     1741 tccaggagaa gtacatgttt tcgtactgct tcggtacgaa ctgaatgtcg ccgttctcaa
     1801 ccgcctcaac cgccggtttc gccagtacat cggcacgcac gtaccactgg tcggtgagca
     1861 tcggttcgat aactacgccg ccacggtcgc cgtaaggcac ggtcaggtcg tgcggtttaa
     1921 tttcttccag caggccgagc gcgtcaaccg cagcaacgac tgctttacgt gcagcaaaac
     1981 gctccagttt ctggaactct gccgggattt cgctggaata aacgtcagat tcgttacctt
     2041 tggtatcgaa cacctgggcg ctttcacgga tatcgccgtc aaaggtcagg atgttgatca
     2101 tcggcagcgc gtgacgttta cccacttcat agtcgttaaa gtcgtgcgcc ggagtgattt
     2161 tcacgcagcc ggtgcctttt tccatgtcgg cgtgttcgtc gccaacgatc ggaatacgac
     2221 ggttaaccag cggtagaatg acatatttgc caatcaggtc tttgtaacgc ggatcttccg
     2281 ggttaacggc cacgccggta tcgcccaaca gggtttccgg acgggtagtc gcaaccacca
     2341 gataatcttt accgtctgcg gttttcgcgc cgtcagccag cggatagcgg atgtgccaca
     2401 tcgaaccttt cgattcgcgg ttttccactt ccaggtcaga gatagcggtg cgcagtttcg
     2461 gatcccagtt taccaggcgt ttgccacggt aaatcaggtc ttctttatac agactaacga
     2521 aaacttcttt caccgcattg gacaggcctt cgtccatcgt gaagcgttca cgctcccagt
     2581 cgacggagtt gccgagacgg cgcatctgac gggtaatcgt gccgccggat tccgctttcc
     2641 attcccaaat tttgtcgatg aaagcttcgc ggccgtagtc gtgacgggtt ttaccttctt
     2701 ctgcggcaat cttgcgctca acgaccatct gggtagcgat cccggcgtgg tcagtaccga
     2761 cctgccacag ggtgtttttg ccctgcatgc gctgatagcg gatcatggta tccatgatgg
     2821 tttgctggaa ggcgtgaccc atatgcaaac tgccggtgac gttcggcggc gggatcatga
     2881 tgcagaaact ttcctggctt tcatcgccat taggcttaaa gtagccctgc ttttcccagt
     2941 gctcgtaaag cggctgttcg atatcttgtg ggttatatgt cttttccatt atttccaggt
     3001 tgccgtattc aggttgaaac cagccacgcg gtaggcttta tagcgttcgc gcgccagttg
     3061 tttcagagaa tcttcataag gaacgaagtc taccacttct gtgaaagcgg tggcaaaatc
     3121 tgcaaagctt gttcgcagac taatcaatat atcgcgcggg ctgctgctgc gtttttgcgg
     3181 ccaggcgatc tccaccggcg caccgccgcg cggtccttct cccgctaaat tatgcggaac
     3241 aaagctttct gccggacgcg cccacagggc ttcatccagc cggtaagcct gcttttcatc
     3301 ttcacaggcg atgagcacgc gcttaccgct gcgccaacgt tctgcggcaa tttcacacac
     3361 cagttgctca acggtgctta agccatcgac ggtggtgtca ttgtccagaa ggtagaacgt
     3421 cgcgtttttc atatatgggg cttcttgtgg tggatttaaa tacgcatcag gtatacaccg
     3481 cagcgcctga tgcgacgctg ttgtgtctta tcaggcctac gagttcagtg ctgtgtaggt
     3541 cggataaggc gttcacgccg catccggcaa taacagcctt gcctgacgca attactcttc
     3601 gccgttaaac ccagcgcggt ttaacaggaa ctgtgccagc aacgccaccg gacgaccggt
     3661 ggcgcctttt gctttaccag aacgccaggc ggttccggcg atatccaggt gcgcccagtt
     3721 gtacttacgg gtaaagcgcg acaggaagca acctgcggta atcgccccac caggacgacc
     3781 gccaatgttc gccatatcgg caaaattgga ctccagctgt tcctgatact cgtcacccag
     3841 cggtaagcgc catgcgcggt caccggattg ttcagacgcg gcaatcagtt catgggccag
     3901 cggattatgg ttcgccatca gaccggtaat gtgatgaccc agcgcgatca cgcaggcacc
     3961 ggtcagcgtc gccacgtcaa tcaccgcttc cggctcaaaa cgctcaacgt aagttaacac
     4021 gtcgcacagt accaggcggc cttcagcgtc ggtattcagc acttcaacag tttgaccgga
     4081 catggtggtt aacacatcgc ccggacgata ggctcgtccg ccaggcatgt tttcgcagcc
     4141 tgccaacacg ccgataacgt taatcggcag ttgtagctcc gcgaccatcc gcatcacgcc
     4201 gtaaaccgct gccgcaccgc acatatcgta cttcatctca tccatgcctt ctgaaggctt
     4261 gatcgagata ccgccggagt cgaaggttaa acctttaccc accagcacga ttgggcgtgc
     4321 atcttccgac gcgttgcctt tgtactcaat caccgacatc agcgattcgt tttgcgaacc
     4381 ctgaccgacc gccagatagg aatgcatccc cagctctttc atctgctgtt cgccgataac
     4441 gcgggtgatg acattcttgc tgtagctgtc agccagctgg cgcgcttgtg aagcgaggta
     4501 agcggcgtta cagatattcg gcggcatatt gccgagatct tttgctgctt taatcccggc
     4561 ggcaatcgcc agaccgtgca ggatcgcgcg ctcaccgctg gtcagttcac ggcgggtcgg
     4621 cacgttgaac accattttac gcagcggacg acgcggttcg ctcttgttcg ttttcagctg
     4681 atcgaaactg tagagcgtct cttttgccgt ctcgacagcc tgacgcactt tccagtagtt
     4741 gttacggcct ttaacgtgca gttcagtcag aaagcagacc gcttccattg agccagtatc
     4801 attcagcgta ttaatggttt tctgaataac ctgcttgtac tgacgctcat ccagctcacg
     4861 ttctttgccg caaccaataa ggagaattcg ctcggaaagt acattcggaa catggtgcag
     4921 caacaatgtc tgccccggtt ttccttccag ttcgccccga cgtagcaggg cgctgatgta
     4981 cccatcgctg attttatcga gctgttctgc aatcggagaa aggcgacgtg gttcgaagac
     5041 gcccacgacg atgcaggcac tccgctgttt ctccgggcta ccgcttttta cactaaactc
     5101 catgcactac gctcctgaat cttaaagaca acggcggtgg ctacagatag aattgcaagc
     5161 tttcgtaact catgtccgct gttgcgatga cttcgtgtta atcttaacgt tattacggca
     5221 ttggcacgtc agaacaaaat ctgagaggtg aatccgttga gtataattat cttagcgacg
     5281 atttcgacga ctcaagagaa taaatgacgt ttaagccatg aaacaagcta aaatcctgca
     5341 aaagacgagt ttttacgggc gtatttaaag tgataatcat aagatatctg gtgcgggaga
     5401 cgctcaaaag ccagctggcg atactcttca tcttgctttt gatcttcttc tgtcaaaagt
     5461 tagtgaggat cctcggcgca gcggttgacg gcgatattcc ggcgaatctg gtgctctccc
     5521 ttctcgggtt gggcgtgccg gaaatggcgc agcttatcct gccattaagc ctgttcctcg
     5581 ggctgctgat gacgctgggc aaactgtata ccgaaagtga aattacggta atgcatgcct
     5641 gcggcctgag caaagcggtt ctggtgaaag cggcaatgat ccttgcggta ttcactgcaa
     5701 tcgtcgcggc ggttaacgtg atgtgggcgg gaccgtggtc atcgcgtcat caggatgaag
     5761 tgttagcaga agcgaaagcg aaccctggca tggcggcgct ggcgcaaggg caattccagc
     5821 aagcgactaa tggcagctcg gtgctgttca tcgaaagcgt tgacggcagc gatttcaaag
     5881 atgtgttcct cgcgcaaatt cgaccaaaag gtaatgcacg tccttctgtg gtggtggccg
     5941 attccggaca tttaacccag ctgcgcgacg gctcccaggt cgtcactctc aaccagggaa
     6001 cgcgcttcga aggcactgca ctgttacgtg atttccgcat tacggacttc caggattatc
     6061 aggcgatcat tggtcaccag gcggtagcgc tcgacccgaa cgataccgac cagatggaca
     6121 tgcgtacatt gtggaacact gacaccgatc gtgcccgcgc agaactgaac tggcgtatca
     6181 cgttggtatt caccgtgttt atgatggcac ttatggtcgt accgctgagc gtggttaacc
     6241 cgcgtcaggg acgcgtacta tcgatgctgc cagccatgct gctgtatcta cttttcttcc
     6301 tgatccagac ctccctgaaa tcgaacggcg gtaaaggtaa gctggacccg acgctgtgga
     6361 tgtggaccgt taacctgatt tatctggctt tggcgattgt tctcaacctt tgggacaccg
     6421 tgccggtccg ccgcctgcgc gccagttttt cgcgtaaagg agcggtgtga tgcaaccttt
     6481 tggcgtactt gaccgctata tcggtaaaac tattttcacc accatcatga tgacgctgtt
     6541 catgctggtg tcgctgtcgg gcattatcaa atttgtcgat cagctgaaaa aagccgggca
     6601 ggggagttac gacgcgttag gcgcaggaat gtataccttg ctgagcgtgc cgaaagatgt
     6661 gcaaacattc ttcccgatgg cggctctgct tggggcgttg cttggtcttg ggatgctggc
     6721 gcagcgcagc gaactggtgg tgatgcaggc ttctggtttc acccgtatgc aggttgcgct
     6781 gtcggtgatg aaaaccgcca ttccgctggt cttgctgacg atggcgattg gtgaatgggt
     6841 cgcgccgcag ggcgagcaga tggcgcgtaa ctaccgtgcg caggcgatgt acggcggctc
     6901 gttgctctct acccagcaag gcttatgggc gaaagatggc aacaacttcg tttacattga
     6961 gcgggttaaa ggtgacgaag agttaggtgg catcagtatt tatgccttta acgagaatcg
     7021 tcgtctgcaa tccgtacgct atgccgctac tgcgaagttt gacccggaac ataaagtctg
     7081 gcgtctgtcg caggttgatg aatctgatct gaccaatccg aaacagatca ccggttcgca
     7141 gacggtgagc ggcacctgga aaaccaacct cacgccggac aaactgggcg tggtggcgct
     7201 ggacccggat gcactctcaa tcagcggttt gcacaactac gtgaagtatc tgaagtcgag
     7261 cggtcaggat gccggacgtt atcagctcaa tatgtggagc aaaatcttcc agccgctatc
     7321 cgtggcggtg atgatgctga tggcgctgtc gttcatcttt ggcccactgc gtagcgtacc
     7381 gatgggcgtg cgtgtggtca ccggtatcag cttcggtttt gtcttctacg tactggacca
     7441 gatcttcggc ccgctgacgt tggtttatgg catcccgccg atcatcggcg cactgttgcc
     7501 aagcgccagc ttcttcttaa tcagcctgtg gctgttaatg agaaaatcgt aaccgaatga
     7561 acgaataaag ggagcgaaag ctccctttat tattgttagc aaagtgtgct tcgttcatgc
     7621 ctgaaaaatc atgaaaatcc aatatattgc agtggtgtag gcatgataag acgcgccagc
     7681 gtcgcatcag gcaaagacaa atctcgcctg acttaccttc ttcttccccc cagcaaactc
     7741 cccaacatcc cacgtacaat ctgattcgtc acctggcggg cggcgctttt cgccattgtt
     7801 tgcaccacac catctttctt cccgccgcgt ggtccggtag tgccaaacaa aatatccttc
     7861 aatccaccaa gaataccgtc atccaccgcc accgctttcc ctttcgcggg aggattattt
     7921 tgctgcttgg tactggcctg aaagcctttt tgcaacatct cataggcgga ttcccggtcc
     7981 acgtcatcct cgtacttgcc atacaccggg gagtggttaa tcaagccatt gcgctcatct
     8041 tccgtcaccg gtcccatccg cgaacaaggc gcaatcacca tcgcccgttc caccacagaa
     8101 ggacttcctt ttgcatcgag aaacgagatt aacgcctcgc cggtccccag ttcctggatt
     8161 gccttttcgg tatcaaacgc cggattggcc cgcatggttt gcgccgcagc ttttaccgct
     8221 ttctgatctt tgggcgtaaa agcccgcaaa gcgtgttgaa cgcgattacc gagctgcccg
     8281 agcacattat ccggaatatc agacgggttt tgcgaaacga accagacgcc cacgcctttt
     8341 gagcgaataa gccttattac ctgctcaatc ttatccagca gtacctgcgg tgcatcgtta
     8401 aacagcagat gtgcctcgtc gaagaaaaac accagttttg gtttctccag atcgcccgct
     8461 tccggcaatt gttcatacaa ctctgaaagc atccacagca ggctggcggc gtacagtttc
     8521 ggcatctgat aaagcttctc ggcgctgagg atattgataa cgcctttacc gttggtatcg
     8581 gtgcgcatcc agtctttgat atccagcatc ggctcgccaa agaaatgcgc cgcaccttgc
     8641 ttttccagcg acagtaaacc gcgctggatg gcaccaaccg atgcgctact gatattaccg
     8701 tactgattct ggaaagattt ggcgttatcg ccgatgtact gggtaatcgc ccgcagatct
     8761 ttaaagtcga gcagcaacaa tccctgatcg tcagcaatac ggaagatgat attcagcacg
     8821 ccagattgca catcgttgag attcaacagc cgcgccagca acagcggccc caggtctgaa
     8881 accgtcgccc gcaccggatg gcctttctcg ccaaagatat cccacaccac caccggatta
     8941 gcatgcggtt gccagtcatt gacgccgata tttttaagcc ttgtgagcag tttttccgac
     9001 gccgttcctg cctgcgcgat accggtcaga tcgcctttca catcagccat aaacaccggc
     9061 acgccgattt ccgacaatga ctctgccagt ttttgcagcg taacggtttt acccgtcccc
     9121 gttgcgccag taatcagccc gtgacggtta gccattcccg gcagtaaaaa aagttctgtg
     9181 tccggcgtgc gggcaattaa taggggttcg ctcatgagat ttcctccagt ttcctgcctg
     9241 gagtataggc aacggctgcg cactcttcac cgctaaattc agcagattgc gagacgcctt
     9301 ccaaagagat ttaagtgccg ggcgacaggc acccggcaaa gggatcagaa gtcggctttc
     9361 agaaccacgc ggtaacgcgc cttaccgtcg cgcacatgct tgatggcatc gttaattttc
     9421 gacatcggga acagttcggt ggtcggcgca accttgctac gggcggcaaa gcgcatcagc
     9481 tttcgcagct cataaggcgt gccggtagca gaaccagaga tgctgcggtc gcccgcaatt
     9541 aatgtaaagg ccggaacaga cagcggcgtg agaaccgcac cgaccgtatg gaaattaccg
     9601 ccataggtca gcgcctcaaa ataaggctgc cagtcgaggc tgacgttcac agtattgata
     9661 atgagatcaa actgccccga cagggctttc agtgcctgcg gatcgcggct attcaccact
     9721 ttatcggcac ccatcgccag tacttcctgc tctttcgccg gattagaact aaaggccgtc
     9781 acctcgcatc ccattgcgtg cagaagtttt atagcgatat gccccagccc gccaatacca
     9841 attaccccaa cgcggctggt agcagtgata tggtgcatca acagtggttt aaagaccgtg
     9901 ataccgccgc acaacagcgg cccggcggac tcgatatcaa tattttcagg cagtggaatc
     9961 acccattgcc agtccgcacg caacttctcg gcaaagccac cgcgattcat aatcgtcggc
    10021 accgcacctt gctcgcagtt gatctgatta ccgctaatac aggcgtcgca gtgaccacag
    10081 ctacgcgccg tccagccaat cccgacacgc tgaccgacct gcaaaccttt atcctgcgcg
    10141 gcgctcccga gtgccaccac gcgcccaatc acctcatgcc cggcaaccag cggatattgt
    10201 gaaaatcccc attcattgtc gatcatcgat agatcggaat ggcagatccc gcaataatcc
    10261 acctgcactt caacatcttg tggcctcagc tcaccgggat cgtactcata aacttccagt
    10321 tcgccgcccg cttcttttgc ggcatagctt tttatcatcg acattttttg gtccttctct
    10381 ggtgttgttt gggaagtgta gagcatggca gggcggggtg cctggagtgt gacaaaggtt
    10441 acacatcgct gtgtgcaatg ctgaaaattt cagcacttag cgaggtgcga gcaagctggc
    10501 gcttgcatgg tggcgtgcga caggtataat ccacaacgtt ttccgcatac ctcttcagtg
    10561 ccgaagtggc gaaatcggta gacgcagttg attcaaaatc aaccgtagaa atacgtgccg
    10621 gttcgagtcc ggccttcggc accattctta cttccaagac catccgagaa agtccaaata
    10681 tccctttaaa atcaatgctt gcagcgattt tttcgtcctg cgtcttccga ggttgtccgt
    10741 tgaaatccgg atatcattgg gggcataatt gggggcatct taacttcgat tagaaatgtg
    10801 cccccaaatg aagctcaacg ccagacaggt cgagaccgca aagccaaaag acaaaaccta
    10861 caaaatggcc gatggtggtg gtttgtatct tgaggtttcg gccaaaggtt ccaaatactg
    10921 gcgcatgaaa tacagacgtc cctctgacaa aaaagaggat cgtctcgcgt ttggtgtttg
    10981 gcctactgtg acgcttgctc aggcaagagc aaagcgcgat caagctaaaa agctgttagc
    11041 gcagggcatt gatccaaaag ccgaacagaa agaagctcag gccgagaatt cgggggcata
    11101 ttctttcgaa actatcgctc gtgaatggca tgctagtaat aagcgctgga gtgaagatca
    11161 ccgatcacgc gttctacgtt atcttgagct ttatatcttt cctcatattg gttcatctga
    11221 catccgtcag ctcaaaacta gccacctatt agccccgatt aaaaaagttg atgccagtgg
    11281 taagcatgac gttgcacagc gccttcagca gcgtgtcacg gctattatgc gttatgcagt
    11341 acagaacgat tacatcgact caaatccagc cagtgatatg gccggtgcgc tatcgacaac
    11401 caaagcgcga cattaccccg ctttgccctc cagccggttc cctgagtttc ttgctcgtct
    11461 tgctgcatat cgtggccgtg taatgacgcg gatcgcggtt gagctttcct tactaacttt
    11521 tgtgcgttcc agtgagttac gtttcgcgcg ttgggatgag ttcgacttcg ataaatctct
    11581 ctggcgagta cctgcaaaac gagaagaaat taaaggtgtg cgttactcgt accgtggcat
    11641 gaagatgaaa gaggagcata tcgttcagct tagtcggcag gcgatgattt tgctaaacca
    11701 gttaaagcag attagtggag ataaagagct gctttttccg ggtgatcatg acgcaactaa
    11761 ggttatgagc gaaaacacgg taaatagcgc attgcgtgcg atgggctatg acacgaaaac
    11821 cgaagtttgc gggcacgggt ttagaacgat ggcccgtggt gcgttgggcg agtccggatt
    11881 atggagtgaa gatgctatag aacgacagtt gagccattca gagcgtaata atgtacgtgc
    11941 tgcttatatt cacacctctg aacatttgga tgaacgtcga ttgatggttc aatggtgggc
    12001 tgattatctc caagcaaata agggcaaaac aataacacct tatgattttg ctaaaataaa
    12061 caaaagtcga tagtgaaaat aaacgcctat aatatatagg cgtttatttc tgtattaaac
    12121 actttgatca taatcttcca gcagcaactt tttttcgtac ttcatctttc caattgccac
    12181 tatggattat tctatcgaaa ttacttcctg ctcttcctga taataagtca attcgagata
    12241 gcttgctgat atcatcccag ttatcataga ggatatatat agcattacca tattctatgt
    12301 tttcaaatac aaccaaatcg tcctccagca tagctccaat ataattactg aacgtgctgt
    12361 ttcctgtgac gattttttta ggttcaagac tcatgaagaa gtcatatcta ttttcagcga
    12421 catttttctt gtcggaagtg atatttttac ctctaaaaat tcttgctaaa gtttcttctt
    12481 tactacctgg tggaaatatt tcccatgaga ctataagtgt atcagcataa gtcgatatgg
    12541 gtttatcagc atcctcaatt ccacatttac caagattttc ttgaagaaga tttaaatcaa
    12601 ataataattc atcatcaaac ttatttgatt gtttgtctaa aattctagtt actctgaagt
    12661 taaatatgaa atggctgtcc gagggctgtt tgtgattaat ttcaatggct atgagtgttg
    12721 gtggtataat gtctctggga tatttctcgt aaggtaatct aacagtatgt gtaccattag
    12781 agctatcacc ccagttaggt gactcaattt cagtgtaatg agtttctttt gggaggtctt
    12841 ttctgacaat ttcttcacca aaaacatttt taagtgaata tttaccctgc aatggatctg
    12901 gtatgaccga cgtctcataa gcattaacac cattatcaaa atatattcca aggtgctcta
    12961 atgctccact ttttaattgg ttttccgtaa atgcaataga agtgccagca acaactgtat
    13021 taccaagcct tcttaacttg ctatgaatat ttttaggtat aacacgtata ttctttttca
    13081 tttttccctc atacttgatc atcaatgtct ctttgctctc gcttgttatg taaatcattg
    13141 ataaacataa catttgcaag tattacgcta attactgcct caatctgcat gtacgaaata
    13201 gcaataatta atgctaaaga tgtgtttttt attacatctt ggctctctaa ataaagatga
    13261 cttttgaaaa acactagttt aactaagtat acaacaagta aagtaatcat aacgaaagat
    13321 gattttagaa cacttccaac tattgcttct aatcttcttg tatcttccag ttgttcggtg
    13381 aaatcaacgg tttctatagt tttggggttc acaatacgct gcaaagcatt tgggtaaaga
    13441 aacgctaacc aaattcccat aatagtaaaa accattccag aaactgatac gagaagagtg
    13501 caatagttgg caaaatcgtt atacacatag ttaatatcgg atgacgaaat aatcatcagc
    13561 gatataatga atatcgccat gtatatccat gtgtatttgg ctatgcctgc tatagctttc
    13621 aatattcaat cctctccaga tgctttcttt attcttggtt gagattgtaa tgcttttaat
    13681 tcttttaata taggggaaat aaattctttt cttttgtgtg atatttgttc ataaatatat
    13741 gcggcggaat atgttgaata gtcgtcagaa gggacattga gaatatcacg cattctatat
    13801 ttatccaccc aagttatacc cttgtctgta gcaaatccaa cattgtccca aagacgtttt
    13861 tctctatcct cagccgaata cttttcaatg atttcttgaa cttcattcaa ggatggtttt
    13921 gcttctgctt ttatttcaat ttgtcttgtt ttggcatttt tctttccgga cacaaatgga
    13981 acgagagtgc taaaggtctt taaccaatca gctcgtgcat catttggatt tataataata
    14041 gtttccctgc gaactatgtg tgttattttt ggtattagtt tacctaactc agtatgagtg
    14101 gtttctaagc ttctgagctt catatcaaac ctgtacatat atttatataa gtcttcgtca
    14161 tcggtattag atagccttat ataacctttc tcggttttat ttttaaccct tttgctatgt
    14221 ttaactctat tagtaataga tgagtgtaca tagtcttgaa atagttcaga atcacaaaca
    14281 gaatggtcga atttaatgga tactatagtt tcaagttcag gtataaccca ataaaagcat
    14341 ggccgccccc agataacttt tttccctctg tagctattag tatatttaac caccgatgag
    14401 cttccgattt caccgtcttc actagctcct aataaagagc ccgtactatc agtatcagat
    14461 ttccataaca tgactaagaa atcaccagta ttttcatcct tataaatatc cttgcaataa
    14521 cactttgaac tgtttggttt ttcttttgga tcccatggta tcgtgagtgc aagagattta
    14581 gttcccaccc aatcctgtat gaggtcaaat gtttcgctaa gttctaaacc ataggtattg
    14641 tcatcattta ccttgtataa accgcactta ttaaccttga agaaattgag atgccctaca
    14701 ttcatgctct ttccttagct tcttatcttg ggatatgtat catgatcaaa aattaagcat
    14761 aaccccattg acttcgtgcg ggcaatgttt tcaccctaga atttgatcgg tttaacgtta
    14821 aatattcata agttacatga gcatttttca cttcttccca attacagacg tcatgagctg
    14881 aatattaata aaataattat cctcacggca tggcgcgcaa tgctctcccc gccacgcccg
    14941 cccgctttgc agggcggttt taatgcagtt gcacagtcac gctcaggctg cgccgggaat
    15001 ggcgcggtct gcagaaaatg aggcaggaaa acgcatgcaa agccatgcac cttatcgatg
    15061 catggctttt ttcagtaaaa acgggcggat tttcggggaa ttttacacag actgacgtga
    15121 tgccagttgc gcacttttac gcgaaaaaat catgttctgc gcaggggtga atttttcacg
    15181 gctgtcatcc accgaagtcg cgtcaggcct gaatccgatg gtcgttaaaa tgtcactatc
    15241 ctgtgcggaa taattaattt tttccccctt cgcaagccag gacagaaggg cttcacgcag
    15301 ggcatctgtg gcacgctgta tggcacagtt tcgggcaatg gccgtcagct cactgtagcc
    15361 catcagctcc ggtgccagtg ccgccgccag tgctgtgccg tgctgctgca taaaatcatt
    15421 cagccggtcg cggatgctga tgtgctgaac ggcttcatgc gaacgaatat aacgaccggc
    15481 agcctgattc acctgccatt ttctgacttc gataatattg cgtaattcgt ccaggcgact
    15541 gacgtttctg ccttccccgg acagaagccg cagatattcc tgttcggccg cagccagctc
    15601 atttttgcgt tgcagccatg ctgctttgtt attctgacag gtgtcaaagg cctgctgtaa
    15661 ggctgtgctt tccatcgtta tctctttctc atcatgctga agaataaaaa tacggtgtgc
    15721 ggcgacggcc ggtgttaacc ggcagccctc attccagacg cagcgaatat gattgtgttt
    15781 ttaagcgtac tggcggcagt tcctgttttt catgcaggct ttctgccagt tcgtccggcg
    15841 tgaccgggcg gacaataaaa cggttgatgg tctgaagcgt tttaaacacc agaccacagc
    15901 ccggatccgt gcacacataa aaacgctcgg tgacttcctg agacagacgc cgcgatgttc
    15961 ttgacagtgc aaggccttta catctccgac aacaatatcc ggtaacaagc attcttttcg
    16021 ggcgtttcat actgccggag gctgacgtca gtgaatcgcg gtatctctgt ttgcctgaaa
    16081 tgtattccat tcctgaatct ttacagtcag agaaaaagct ttcactcgct tcaaatgtcg
    16141 cagagcaata aatattccgg cactgtgcaa tcattatctt ggtgccatcg tccatgaaat
    16201 gtgcgcgacg ggtgtgagca acatgtccac acgacgggca gtaaatcatg acagcagtcc
    16261 tctggcctta agctctgctc cctgctggtc tattttgtcc tgccacacct tgcgctgtgc
    16321 cggtgtgcct gccacctcat aatccatgtg cgggagtgtt gccgctgaca gtccggtcag
    16381 ccggagaacc ggctcgccgg tgaggctgat ttgcatctgt ttaattttct gttccagcga
    16441 tgatttcacc tgctgcatga cagccttttc cggtgcgacg tagccctgat ggccggtggt
    16501 gttggcgagc ggattttcct gtaccaggat actcagatgc attgcccgga caagcgcctc
    16561 acaggtttca ttcagggcgt gttccagctc atgctctgca tacagactca gcaggtgatg
    16621 atgtgccttc cggtaggcgg tggccgtgct gtcacacgcc cctttcaggc gttcacgttc
    16681 aaaattcagc accacggcca gattgtcata ttcctgtacc agctcccggc gtgccacgcg
    16741 ctcaatgtgg cgctgtttca gctcgtcgct caggacacca ccggctgcac gaaaggccgt
    16801 gcgccagtcg tcagcgtcat ttccgtcggc ctgcgccagc gcattttttt cctgctctgc
    16861 ccgttcaatg gccgtgacgg tctcatccat caggcgggcg ttctcaaggt gggcggctct
    16921 ggccttttcc agttgtgcca gtgcgggttg cagatattca gggatggtgt tgtcagacat
    16981 tttccggctc ctcgtcactt caggttaaga aaattgtgac gtacaccgga caacaacacg
    17041 acgcattgca gatgtgccag ccctgacaca ggagactcat cctcagaccg gcaagccagg
    17101 aaaaggtcgc aggaaaaacc ggcttactgt ttgttttttt atattttact gttcacctct
    17161 gttcaccata agaaaaaaga taagtaatac agtaagttaa agggtgaaca atcgcagtaa
    17221 tgactgttca ccgtctgttc accactgttc acccgctcat agactttttg tgctgtttat
    17281 tactttttat ttttattaat ccactgaaat aaataagaaa aaacaatttg tatttcacta
    17341 taattttttc cagctccttc cagagcgttt taaagccatc tggcgctgat gtataaaaaa
    17401 tacacagcca ttgtaagact gccggaacaa atcccccctg ttgcatctgc caaaaatatt
    17461 cacaaaataa agcgctaccc gaagccggac ggacttatcc ggtgctgtat ggacattaac
    17521 gaggtagccc gatgcaagct gttttttctt ccccgtctcc cgcccctgtg acgccactga
    17581 tgccgctgcc ggacatcacg caggagcgtt ttttacgtct gccggaagtg atgcacctgt
    17641 gcggcctgtc acgctcgacc atctatgaac tcatccgtaa gggggaattt ccgccgcagg
    17701 tgagtcttgg cggtaaaaat gtggcctggc tgcactctga agtcaccgca tggatggccg
    17761 gacgcattgc cggacgcaaa cgggggtacg acgcatgatg atgcccgctc tgcaaaaact
    17821 ccctttttct ggcttgcctt tttccggcat ttgcggatat agtttttccg ctgccgcaaa
    17881 atcggcagcc gggcgtgaga acccgagcaa ttcacaggcg acatatgacg cgccacgcgt
    17941 ctttttttgt gtcgcaatca atgccacaga gcgccagatt atggtgtggt gtgtggtcag
    18001 tcgtgcagat ggcattccgt gcatcctgcc cgcgtccgct cattatgccg cagagtcaat
    18061 ggtagctcag gcggggcagc ctccgggctg gccggtatcc tgtgaagccg gtattctcac
    18121 ccccgtctgg gctatcgcca tcgagcgtga gaactccggc gatagcgtta tttgctattc
    18181 acaggaggct gccatcatgg ctacaaccct taccccctca caccctgaat ttgtctttgt
    18241 gtttgcggct gtccgtcgcg cagaccgtca tccccgtatc tgtatgcttc gcaccgtggc
    18301 cggtgatgaa cgcagtgccc gccgttccct tgtccgtgac tatgtgctct cccttgctgc
    18361 ccgtctgccg gtggtggagg tgtcccgtgc gtaataaaaa agcccctcag accgtctcag
    18421 cacgtcatga cgcccgtgaa cacctcagca ttgaggctta ccataagctc aaccgcgcca
    18481 gcgccgtatc ccagtttgtt gggggtgatt tgattcaccg tgaactctcc ggcctgcatc
    18541 agctctacat tccgcatatt ttcagctacc tgaatgaaga tattgatttt gtgctgaatg
    18601 agctgaaagc caaaggcctg tgccgcgatt ttctcgccca gcagaaagac cggggagaca
    18661 ggacgcatgt ttgattttcc ccagcccggt gagatttacc gttctgccgg ttttcccgat
    18721 gtggccgtgg tcggcattct ggaagacggt attccgtggg aaatgccgta ccgctgcccg
    18781 gacattgtct ggaacccgta ccgccgtaaa ttcagtatcc ttgtgcgtat cctcgccgac
    18841 gggcgcacca cagacatccc gctggggcgt tttctgcggg aatttacctg tgaccgtcct
    18901 gacctgttta aacgcagccc cgtaaaccgg catgcggtac tgaaagaaat ggccggagac
    18961 ccggaattac agaaatggcg ggagaaatat caggatattt acccgcagga cactgttccg
    19021 gtcagccggg cagcaccggt agcgcgggaa tggcgggaaa ttccccgcac ggagcctgac
    19081 ccggaaatca ccccggataa cagttaccgc aattatctgt aattaaaaaa cgacacccga
    19141 aaaattaaat gtgcgtattc gcgcagggat acgcacgtct tcaggagacg cagatatgcc
    19201 ttatcagtta atgcaaccgg cacggaatgc agtcatctgt cacagggagg aaagcaaatg
    19261 aaaacaccct actaccgccc gtcttacgcg cagcccttta ccgtcgtgct gtcgcctgtg
    19321 cctggctgac cgtgtgcgaa cgtcagcacc gctacccgca tctcaccctt gagtcactgg
    19381 aggcggccat cgccgctgag ctggaaggct tttatctgcg ccagcacggt gaggaaaaag
    19441 ggcgtcagat agcctgtgcc ctgctggaag atttaatgga atccggcccc ctgaaggccg
    19501 caccgtcgct gtcctttctc gggctggttg tgatggatga actctgtgcc cgtcacataa
    19561 aagcgccggt actgcactga aggagaacaa caccatgaaa atgaacgtaa ccgccaccgt
    19621 cagccatgcg ctcggccact ggccgcgtat tctcccggcg ctggggattc aggtgctgaa
    19681 gaaccatcat cagccctgtc cggtctgtgg cgggagtgac cgcttccgtt ttgatgacag
    19741 ggaggggcgc ggcacctggt actgcaatca gtgtggtgcc ggtgacggcc tgaaactggt
    19801 tgaaaaggtg tttggtgtct ccccgtccga cgtggccgca aaggtggctg ccgtgaccgg
    19861 cagtctgccc ccggctgacc cggcagtgac ggccgccgcc gttgctgaaa cagacgctgc
    19921 ccggaagaac gccgccgcac tggcacaaac cctgatggca aaaacccgtc ccggaaccgg
    19981 taacgcctac ctgacccgca agggctttcc cggccgggaa tgccggatgc tgaccggcac
    20041 acacagagcc ggtggcgtga gctggcgcgc cggtgacctt gtggtgccac tgtatgacga
    20101 cagcggcgaa ctggttaacc ttcagttaat cagtgctgac ggccgtaagc gcaccctgaa
    20161 aggcggacag gtcaggggca cctgtcacac ccttgaagga cagaatcagg ccggaaaacg
    20221 tctgtggata gcggagggat acgcgaccgc acttaccgta catcacctga ccggtgaaac
    20281 ggtgatggtg gcgctttctt ccgtgaacct cctttctctg gccagccttg cccggcagaa
    20341 gcatccggcc tgtcagattg tccttgccgc agaccgtgac ctcagcggtg acggccagaa
    20401 aaaagccgcc gcagccgcag atgcgtgtga aggtgttgtt gccctgccgc cggtcttcgg
    20461 tgactggaat gatgccttca cgcagtacgg cggggaggtc acccgtaagg ccatttacga
    20521 tgccatccgg ccaccggctg aaagcccgtt cgacaccatg agcgaagcag agttttccgc
    20581 catgagtacc agcgaaaagg ccatgcgtat ctatgagcat tacggcgagg cgctcgcggt
    20641 cgatgccaac ggccagcttc tgtcccgcta tgaaaatggt gtctggaagg tgctgccgcc
    20701 actggacttt gcccgggatg tggccgggct gtttcagcgt ctgcgcgcgc cgttctcctc
    20761 cgggaaggtg gcctccgtgg tggacaccct gaagctgatt attccgcagc aggaagcccc
    20821 ctcccgccgc ctgattggct ttcgtaacgg tgtgctcgac acacagaacg gcacgttcca
    20881 cccgcacagt ccgtcacact ggatgcgtac cctgtgcgat gtggatttca ccccaccggt
    20941 ggaaggggaa acgctggaaa cccacgcccc cgcgttctgg cgctggcttg accgtgccgc
    21001 cggtggccgt gcggaaaaac gcgacgtgat tctggccgca ctgtttatgg tgctggcaaa
    21061 ccgctacgac tggcagctct ttctggaggt gaccggtccc ggcggcagcg gcaaaagtat
    21121 catggccgaa atcgccaccc tgctggccgg ggaggataac gccacgtcgg ccaccattga
    21181 gacgctggaa tccccgcgtg aacgtgccgc gttaactggc ttctcactga tacgcctgcc
    21241 ggaccaggaa aaatggagcg gcgacggtgc cggactcaag gccatcaccg gcggcgatgc
    21301 ggtgtccgtg gacccgaaat accgggatgc gtactccacg catatcccgg cggtgattct
    21361 ggccgtgaac aataacccga tgcgcttcac cgaccgcagc ggcggcgtgt cacgccggcg
    21421 ggtgattatt cacttcccgg aacagatagc cccgcaggag cgcgacccgc agcttaagga
    21481 caaaatcacc cgcgagctgg cggtcatcgt gcgtcacctg atgcagaagt tcagcgaccc
    21541 gatgctcgcc cggtcactgc ttcagtccca gcagaactca gacgaggcgc tgaacatcaa
    21601 acgggatgcc gacccgacgt ttgattttat cggctatctg gaaaccctgc cgcagaccag
    21661 cggcatgtat atggggaacg ccagtatcat cccgcgtaat taccgtaaat acctctatca
    21721 cgcctatctg gcctacatgg aggcaaacgg ctaccggaat gtactcagtc tgaaaatgtt
    21781 cgggctgggg ctgccggtga tgctgaagga atacggactg aattacgaga agcgccatac
    21841 caaacagggg atacagacca acctgacact gaaagaggaa agctacggcg actggctgcc
    21901 aaaatgtgac gaccctgcaa caacctgacc cacctgaccg gcatctgccg gtcttttttt
    21961 atcccgacct cccccgaagg tgaacaatcc actgttcacc cttcaccgta tattcacccg
    22021 ttatcacact gaaattaaaa gagaaaaatg aaaggtgaac agtgtgaaca gttctttcga
    22081 aaaaaaattt tttatgtgga taaaagatgc actggtttgg atccgggcta cggatccaaa
    22141 cttactgatt aaaaattatt taactttccg atttattgat gggctgaaaa tagattggtc
    22201 agaaaaacag tgggggcaca aaagggggca tatttcattg ttgtgtttta ttatttgttt
    22261 tttatcatta acttattttg atatttgagt ccggccttcg caccaaaagt atgtaaatag
    22321 acctcaactg aggtcttttt ttatgcctga aatccgcgct acacaaggct tttccagctt
    22381 ttcccctcaa ctgaagtcaa cctgactcaa cccacatcaa caggcttgtg agtatactaa
    22441 tgagtatatt tgccgattcg atattgcttg tatactcacg agataccaat ggaaggaaga
    22501 ccattatggc cctaacggat actaaagttc gctctgcgaa acctgaggag aaagagtatt
    22561 cacttgttga cggcgacggc atgtccttac ttgtaaaacc tggcggttca aagtattggc
    22621 gatttcgttt ccgttttggc ggtaaacaac atttgatggc gttcggcgtt tacccggatg
    22681 tttcgctggc ggatgcgcgg aagaaaaggg aagaggccag aaagctggta gctgctggta
    22741 tcgatcctcg tgagcataag cgtgctgtga aagaagagca ggcgaaagag attattactt
    22801 tcgagaaggt tgccagagag tggctcgtaa ccaaccaaaa atggtcggaa gagcatgcta
    22861 atcgtgtaaa aaagagcctg gaggacaata tcttcccggc aattggttcc cacaatattg
    22921 ctgaactggg cacccgcgat ctgttaattc ctattaaagc cgtggagaaa tccggacgtc
    22981 ttgaagtggc ttcccgcctt caacagcgca ccacggccat catgcgttat gcagtgcaaa
    23041 gcgggttaat tgattacaat ccggcgcaag agatggcggg ggcggtagct tccagtaatc
    23101 gacagcatcg tccggcgctg gagttaaagc gtatcccgga attactccag aaaatcgatg
    23161 aatacaccgg caggccacta acccgctggg caaccgaact taccctgctt atttttattc
    23221 gatccagtga gctgcgtttt gctcgctggt cagaaatcga ttttgaaacg tcaatgtgga
    23281 ccatcccgcc tgagcgagaa cctattcccg gtgtgaaaca ttctcagcga ggagcaaaga
    23341 tgcgtacacc tcatctggtg ccgctttcaa agcaggcgct agcgatcctg aagcagataa
    23401 aacagttctg tggcgaacat gagctgattt ttattggcga tcacgatccg agtaagccca
    23461 tgagtgaaaa cacggtgaac agtgcattgc gggtgatggg ctatgacacc aaggttgagg
    23521 tttgcggcca cggcttccgc actatggcct gtagctcatt gattgagtca gggttatggt
    23581 cgaaggatgc ggtggaaagg cagatgagcc atatggaacg caattcggtg agggcggcgt
    23641 atatccataa agcggaacat ctggatgagc gcaagctgat gttgcagtgg tgggctgatt
    23701 ttctggatgc gaaccgggag aaggggatta cgccgtttga ctatgcgaag atcaatcggg
    23761 gtaatggcga gtgagttatc taaaagcgca cattcgcctc taaattgaaa gctgctgact
    23821 tattcaaacg aggaattggt cagcagtgcc aaatagccga gttttcacta aggtaagcct
    23881 ttttattgaa cctaatcggc agtaatgctc cacctaagcc taaatttaaa gtcatgatta
    23941 cttataaaag gggagtggac ccttgattga gccctacatc gtaacaaagg tacagcaatt
    24001 cggctacatc aggagtgata gtcaattagt taatctaata attaattgaa tctaaataat
    24061 taaatttctt acgtaaaatt gaccaattta ctggtggtgt aagccgcaat atcttaatgt
    24121 aatcagtata aaggggcttg atttctttta aagatagtat ccgtatgaat tttaagaaaa
    24181 aactcgaaga gcattttaag cagttcgaag catccccagt gttatttgtc ggctccggtg
    24241 tgtctcgtcg ttatcttggt gtgccatgtt ggcaagacct tttgaagcat tttgctgagg
    24301 caattgggga aaatcatata aaattaaaaa caaaatctaa tggtgactta cccgaatatg
    24361 cacaattatt ggtgtctgca tatgctgaga aatggtggga tactgaagaa ggacaattag
    24421 cattaagtga aaaagaacag gagaaaactt tcattaacga acagtctcct ttgaagttgt
    24481 caatatctaa atatattgag aatgcacata aaaatattat agataatgat gagt
//
LOCUS       DAJVOY010000049        24084 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVOY010000049 DAJVOY010000000
VERSION     DAJVOY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.049"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            322..549
                     /gene="feoA"
                     /locus_tag="OQL90_001728"
     CDS             322..549
                     /gene="feoA"
                     /locus_tag="OQL90_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1368540.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            566..2887
                     /gene="feoB"
                     /locus_tag="OQL90_001729"
     CDS             566..2887
                     /gene="feoB"
                     /locus_tag="OQL90_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1368541.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2887..3123
                     /gene="feoC"
                     /locus_tag="OQL90_001730"
     CDS             2887..3123
                     /gene="feoC"
                     /locus_tag="OQL90_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1368542.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3326..4204
                     /gene="rpnA"
                     /locus_tag="OQL90_001731"
     CDS             3326..4204
                     /gene="rpnA"
                     /locus_tag="OQL90_001731"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCS1368543.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="OQL90_001732"
     CDS             complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="OQL90_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1368544.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5041..5724
                     /gene="gntX"
                     /locus_tag="OQL90_001733"
     CDS             5041..5724
                     /gene="gntX"
                     /locus_tag="OQL90_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1368545.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5783..6358
                     /gene="nfuA"
                     /locus_tag="OQL90_001734"
     CDS             5783..6358
                     /gene="nfuA"
                     /locus_tag="OQL90_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1368546.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6719..8035
                     /gene="gntT"
                     /locus_tag="OQL90_001735"
     CDS             6719..8035
                     /gene="gntT"
                     /locus_tag="OQL90_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1368547.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="OQL90_001736"
     CDS             complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="OQL90_001736"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1368548.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10174..12567)
                     /gene="malP"
                     /locus_tag="OQL90_001737"
     CDS             complement(10174..12567)
                     /gene="malP"
                     /locus_tag="OQL90_001737"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1368549.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEISEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13179..15909
                     /gene="malT"
                     /locus_tag="OQL90_001738"
                     /pseudo
     CDS             13179..15909
                     /gene="malT"
                     /locus_tag="OQL90_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(15952..16968)
                     /gene="rtcA"
                     /locus_tag="OQL90_001739"
     CDS             complement(15952..16968)
                     /gene="rtcA"
                     /locus_tag="OQL90_001739"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1368550.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(16972..18198)
                     /gene="rtcB"
                     /locus_tag="OQL90_001740"
     CDS             complement(16972..18198)
                     /gene="rtcB"
                     /locus_tag="OQL90_001740"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1368551.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18387..19985
                     /gene="rtcR"
                     /locus_tag="OQL90_001741"
     CDS             18387..19985
                     /gene="rtcR"
                     /locus_tag="OQL90_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS1368552.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19967..20725)
                     /locus_tag="OQL90_001742"
     CDS             complement(19967..20725)
                     /locus_tag="OQL90_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1368553.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20742..21572)
                     /gene="glpG"
                     /locus_tag="OQL90_001743"
     CDS             complement(20742..21572)
                     /gene="glpG"
                     /locus_tag="OQL90_001743"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1368554.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21617..21943)
                     /gene="glpE"
                     /locus_tag="OQL90_001744"
     CDS             complement(21617..21943)
                     /gene="glpE"
                     /locus_tag="OQL90_001744"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1368555.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22133..23638
                     /gene="glpD"
                     /locus_tag="OQL90_001745"
     CDS             22133..23638
                     /gene="glpD"
                     /locus_tag="OQL90_001745"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1368556.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23690..>24084)
                     /locus_tag="OQL90_001746"
     CDS             complement(23690..>24084)
                     /locus_tag="OQL90_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368557.1"
                     /translation="VMAGIGWQFSRRTPPELKNRLHCWLVFSPVYFWLILSNFILYIS
                     PEKSALLGNIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGFALGYYCHWCRKNR
                     NQRKKWGDWVTCLSLAILAQGPLFNYLQ"
BASE COUNT         5658 a         6559 c         6095 g         5772 t
ORIGIN      
        1 caaactcata atatattccg gcaatattta tcacttcatt aacaactgaa accttaatta
       61 aacattagcc agtccgggta attcactatt cgaattatat tttcgctgcg atataacctt
      121 gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgcaca cattttaagt
      181 attgctgata gaaaccattc tcattatcat tgtgttattg attatttaat ctctcctttg
      241 ttggcaaatc atctggtctc atgtcgctgt caaacgcccc atgaggtagt tatccagtta
      301 atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa aatcactggc
      361 ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctcttgg catgttacct
      421 ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg accccattca tatcgaaacc
      481 cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt ggaagcggtt
      541 tcctgttaat acggtgataa caacaatgaa aaaattaacc ataggcttaa ttggtaatcc
      601 aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc gtgtaggtaa
      661 ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg atcatcaggt
      721 cacgctggtg gacctacccg gcacctattc tctgaccacc atctcatcgc agacctcgct
      781 cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc tgattaacgt
      841 ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctacaactgc tggaactcgg
      901 cattccctgc attgtggcac tgaacatgct cgacattgcc gagaagcaaa atattcgtat
      961 tgaaattgat gctctgtcgg cgcgtctggg ctgtccggtg atcccgctgg tttcaacccg
     1021 tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta acgagaatgt
     1081 ggaactggtg cattacgcac agccgctgct caacgaagca gattcactgg caaaagtgat
     1141 gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga
     1201 tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgccg ccctcgcccg
     1261 tctgcgtaat gagatggacg atccggcgct gcacattgcc gatgcgcgtt accagtgcat
     1321 tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc gtttcaccac
     1381 tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc tctttgtgat
     1441 gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc tgtttgacgt
     1501 cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc tccacttccc
     1561 ggactggctg actatcttcc tcgcccaggg cctgggtggc ggcattaaca ccgtgctgcc
     1621 actggtgccg cagattggca tgatgtacct gttcctctcc ttccttgagg actccgggta
     1681 tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag gcgctgggct tgccggggaa
     1741 atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa tgggtgcacg
     1801 tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt ttatgtcctg
     1861 cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc
     1921 gctggcggtc ttctcgctgt atatgctggg tattgtgatg gcggtgctga ctggcctgat
     1981 gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagt tgccggtcta
     2041 tcatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga aaggcttcgt
     2101 tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc ctgagcgctt tcaacagctt
     2161 ctcgctgagc gggaaaatcg tcgataacat caacgactcg gcgctggcgt ccgtcagccg
     2221 ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa gataactggc aggcaacggt
     2281 tggcctgttt acaggtgcca tggcgaaaga agtggtagtg ggtacgctca acaccctcta
     2341 caccgcagaa aatattcagg acgaagagtt caatccggca gagtttaacc tcggtgaaga
     2401 gctgttcagt gcgatagatg aaacctggca gagcctgaaa gacaccttca gccttagcgt
     2461 actgatgaac cccattgaag ccagcaaagg caacggcgaa atgggtaccg gggcgatggg
     2521 cgtgatggat cagaaattcg gtagcgcagc agcagcttac agctacctga ttttcgtcct
     2581 gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg
     2641 gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcgctggcaa cattgttcta
     2701 tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc tggcggttat
     2761 cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg cgtagccggg tggatatcga
     2821 actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca ccggtgattg
     2881 ccattaatgg cttcacttat tcaggtgcgc gatttgctgg cgttacgggg ccgtatggaa
     2941 gcggcccaga taagccagac attgaacact ccacagccaa tgattaacgc catgctgcaa
     3001 caactggaaa gtatgggcaa agccgtgcgg attcaggaag aacctgacgg ctgcctctct
     3061 ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc gcgagtggtg ggcgcttcgt
     3121 taaccttact ccatcgcctg ttttggaaag cccggtatgc gtctgcatcc gggctttttt
     3181 gcgtgcggct ttccataaaa atgcaactct tgcagcacgg cgtaagttcc tttgaaagca
     3241 tctcgcaggg atgaaaactc gctaatacac aggtgtggag tggcgcgtag agtcgcggca
     3301 ttcaaacaac aggtgaagga acgccatgag caaaaagcag agttccaccc cacacgatgc
     3361 gctgttcaaa ctctttttac gccaaccgga cacggctcgt gattttcttg cgtttcattt
     3421 accggcaccc attcacgcgc tttgtgatat gaaaaccctc aagctggagt cgagcagctt
     3481 tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga aaacggaaca
     3541 aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca aactgatcgc
     3601 atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg ctggatacaa
     3661 aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc cctatcccta
     3721 ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc tttatgcctc
     3781 cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg
     3841 tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgacctga tggggctagt
     3901 agagcaaatg ggctgcttat taagtagtgg atacgctaat gacagacaaa tcaaagggct
     3961 gtttaattac atactgcaaa ccggcgacgc ggtacgtttt aacgatttta tcgacggcgt
     4021 tgccgaacgt tcaccgaaac acaaggagag tttaatgaca attgcggaaa gattgcggca
     4081 ggagggggaa caatccaaag ccctgcatat agccaaaata atgcttgaat ccggagtccc
     4141 tcttacagac atcatgcgct ttaccggggt gtcagaagaa gagttggctg cggcgagcca
     4201 gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc tctgcttcaa cgccaccagc
     4261 aggtgacaaa actcgaccgg atgcgaaata aacggcgcat gggccgcttt ggcgaagata
     4321 tatgatttgc tgcgaggcca gagtttatcc agcatcggca ccactttgcg cggcaccaga
     4381 ccatcgagat agccatacaa tcgcaaaaac ggcatgggca cgttttgcag cggctgacgg
     4441 agatcaaccg ttttcaggat ttccagcccg ccattaagca cgtcaacctc cggcatcggt
     4501 aacgccagaa cggttttctt caacgcccgc gcatcctggc gcgccgtttc agtccccatg
     4561 gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat catcactgag ttgctgctga
     4621 aatcccgcca gcacgtccgg ttttatcccc ggccaatcgt cacgagcact aaaacaaggt
     4681 gacgacgcca cggtgaccag cgcctgaaca cgctcgggat gggttaacgc aatctggctt
     4741 gccaccagcc cgcccagact ccagcctaac caaatggctt tatcaggtgc ctgttgcagc
     4801 acggcttcgg ccatatcagc aagtgacagc gcaccaaatc cctggctgcg cccgaagccg
     4861 ggcagatcaa caaggtgcag cgtaaaatgc gagctaagtt cctcgtcaat gcaacgccac
     4921 acttcggcat tcagtcccca tccgtgcagc agcacaagat gaacattccc ctgacctttg
     4981 gtctgccacc agatgttatt catccgctat tgttctcttt tgacttacaa ggatgaacat
     5041 atgctaacag taccgggatt atgctggcta tgccgaatgc cgctggcgtt aggtcattgg
     5101 gggatttgtt cggtctgctc acgcgccgcc cgtacagata aaacgttatg cccacaatgt
     5161 ggattacccg ccacacactc acatcttccc tgcggtcgct gcctgcaaaa accaccgccc
     5221 tggcaaagac tggtcacggt tgccgactat gcgccgccgt taagtccgct tatccaccag
     5281 cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt cacgtctgtt actattggaa
     5341 gtcttacacg ctcgtcgcac caccgggttg caattgccgg atcgcatcat cagcgttccg
     5401 ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga gcgatttgct gtgtcagccg
     5461 ttatcacgct ggttgcactg ccgatgggat agcgaagccg tcacacgaac acgggccact
     5521 gcgacccagc attttctcag tgcccggcta cgcaagcgca acctgaaaaa tgcctttcgt
     5581 cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg atgatgtcgt taccaccgga
     5641 agtaccgtcg cagagattgc gcagttgctt ttacgcaatg gtgcggcgac tgtccaggtc
     5701 tggtgccttt gtcgaacctt gtagagcctc gatgataggc gtattataac caactaaaat
     5761 agtcaactat taggccatta ctatgatccg tatttccgat gctgcacaag cgcactttgc
     5821 caaactgctg gcaaaccagg aagaagggac acaaatccgc gtatttgtga ttaaccctgg
     5881 cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg gatgccgtgg aagccaccga
     5941 cactgccctg aaatttgacc tgctgaccgc gtatgttgat gagttaagcg ctccttacct
     6001 ggaagatgca gagatcgatt ttgttaccga ccagttgggt tctcagctaa cactgaaagc
     6061 gcctaacgcc aaaatgcgta aagtggcaga cgatgcacca ctgatggagc gcgtggagta
     6121 tatgctgcag tcgcagatca acccacagct tgctggtcac ggtggccgcg tttcactgat
     6181 ggaaatcacc gaagacggtt acgccattct gcaatttggc ggcggctgta acggttgttc
     6241 catggtcgat gtgacgctga aagaagggat cgagaagcag ctgctaaacg aattcccgga
     6301 gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc gaacactcct actactaagt
     6361 tatcccctct tttgtagatt gcccgatgcg acgctaaagc gtcatttcgg gcctacgttg
     6421 aacatgtgcc agacgtgggc gcagcattgc tttggcaaat ccctcccttc gtttctccgc
     6481 gataatatga ccaacctctc ataatttaaa tttactccgc tctggtgatt ctcaaacgcc
     6541 agatgttacc ggtatcattc acatgggtac caaacatact cctgacatct gactacaata
     6601 attagtttta gtgggtatca gtcgtggtgc cgcaatatct ctgttcccga ttgggataat
     6661 tagagtttgt cgtcagaaaa ttgacgttac ccgtaacaaa tgaaaggcca ggtaaatcat
     6721 gccattagtc attgttgcta tcggtgtaat cttgttgttg ctcctgatga tccgtttcaa
     6781 aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct gttggattaa tgcaagggat
     6841 gccgctggat aaagttattg gctccatcaa agccggtgtc ggcgggacgc tcggtagcct
     6901 tgccctgatc atgggttttg gcgcgatgct gggcaaaatg ctggcagact gcggtggcgc
     6961 acaacgtatc gccaccaccc tgattgccaa atttggtaaa aaacacatcc agtgggcggt
     7021 ggtactgacc ggttttaccg ttggttttgc cctgttctat gaagtgggct ttgtgctgat
     7081 gctgccgctg gtgtttacca tcgcggcttc tgcgaatatt ccactgctgt atgttggtgt
     7141 accaatggca gctgcactgt ctgtgaccca cggcttcctg ccaccgcatc cgggtccgac
     7201 tgcgattgcc accattttca atgccgacat gggtaaaacc ctgctgtacg gtactattct
     7261 ggcaatcccg accgtgattc tcgccggtcc ggtttacgct cgcgtgttga aaggtatcga
     7321 taagccgatc cctgaaggtc tgtacagcgc gaaaaccttc agcgaagaag agatgccgag
     7381 ctttggcgtc agcgtctgga cctctctggt gccggtagtg ctgatggcga tgcgtgcgat
     7441 tgccgaaatg atcctgccga aaggtcacgc tttcctgccg gtagcggagt tcctcggtga
     7501 cccggtaatg gctacgctga ttgccgtgct gattgcgatg ttcacctttg gtctgaaccg
     7561 tggtcgttca atggatcaga ttaacgacac gctggtttct tccatcaaaa tcattgcgat
     7621 gatgctgttg atcatcggtg gtggcggtgc gttcaagcag gtgctggtag acagcggcgt
     7681 ggacaaatac attgcttcca tgatgcacga aaccaacatt tctccgctgc tgatggcctg
     7741 gtcgattgct gccgtactgc gtatcgcgct gggttccgca accgttgcgg caatcactgc
     7801 gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt agcccggagc tgatggttat
     7861 tgcggtgggt tccggtagtg tgattttctc tcatgtgaac gatccgggct tctggctgtt
     7921 caaagagtac tttaacctga ctatcggcga gaccatcaaa tcctggtcga tgctggaaac
     7981 cattatctcg gtgtgcggtc tggtaggctg tctgctgctg aatatggtga tttgataaca
     8041 ctgactgccg gatgcatcgc atccggcatc aaccgcactc tactgcttct tcgctgcagc
     8101 tctgcgccgt ctgtccaaat ccttcagcaa cttattcacg ccatcatcgg caaacatcgt
     8161 ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac tggtaactgg tgccaggaat
     8221 attcaccggt tcggccatat ccagccagtc ttccggctgt agtcctaaca gagcactgtt
     8281 actgtcggca atgtagcgct gcaaaccacg attaagcgtc ggcgtcatcg acatcaacga
     8341 tgccttatgc ccggcacgtt ttggcagaca accatattta tgcagtgcat ccagcagccc
     8401 ttgcttcgcc agttcgcgat cctgatacag accgcgcagt accacttcat ccggatacag
     8461 ccccagggtt ttgcccaacg ttagatcccc gctctcccag taaccgcgca gcgtaggcag
     8521 gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac gctttcggtg cacggaacgt
     8581 cttctcgtgg tcgttttcga aatagagcac tttgtaggag tacacaccgc tgctgcgcag
     8641 cttaccgaca atctctaccg gtacggtacc gagatcttca ccaatcacca tacagcgatg
     8701 acgtttactt tcgagtgcca gaatcgagag cagatcatcc accggatagt gaacatacgc
     8761 gccctgatct gccgtctcgc catacggtat ccaccacaaa cgcagcatcg acatcacatg
     8821 gtcaattcgt aatgcgccgc agttttgcat attggcacgc aacagctcga taaacggttc
     8881 ataggcacgc gcggtgatga tatgcgggtc cattggcggt aatccccagt tctgccccaa
     8941 cgggccgagg atatccggcg gcgcgccaac cgacgctttc aggcaatata gttcacggtc
     9001 acaccaggtt tccgccccac cttccgctac gccaaccgcc agatcacgat acaagccaat
     9061 cggcatttca tagccctggc ttatctccca gcaggcggca aactggctgt aagccagcca
     9121 ctgcaaccag agataaaaat cgacgtcatc acgatgttct tcgcagaact gacgcacttc
     9181 tggtgaatcc acgttctgat acatctctgg ccatgcaggc cagccccagc gcatttcgtc
     9241 ctctttcact tgctgggcat gtagcgcatc aaaggctgcc tgccagaaca ggctgtcgcc
     9301 ctgctctgca acaaactggc gaaacgcggt catctgctca tcatcacgtt gcgcgaaacc
     9361 tttccacgcc attcgtaatg ctgtcatttt tagggcggta accgtggagt aatcgaccca
     9421 gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc ggcaactgcc accaggcctg
     9481 agcctcttcg ctaagatgga aatcttcaac ggcgttaacg tcgatataaa tcacattcag
     9541 ccaacggcga gaagacggac tgtatgggct ggcactctcc ggatttgccg gatagagcgc
     9601 atgaatcggg ttcaggccga tgaacgaccc gccacgtttt gccacatcca ccagcatcgc
     9661 tttgagatcg ccaaaatccc caatacccca gtttttttcc gatcgcagcg tataaagctg
     9721 aacgcaggca ccccacagct tttgtttatt cagcaacgcc tgcggttcgt aacagcgttt
     9781 cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc tgggtgagtg tcagcgtgtg
     9841 ataaccttcc ggcagcttcg tcggtagatt gaacgctttg ccccccgtta catggccttt
     9901 gtactgtgtt ccttcttcgg tggtcagcag ccagctatat tcgccgctgc cctccaccac
     9961 catcggcatt tttttgccgc tggtataaac catgacattc gggactggcg ttaccgccac
    10021 tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc cgtttggttt cggcgctaat
    10081 cgactgcggt ttaccgtggg cattgatgta attggggcta atccccgccg ccagcgcggc
    10141 attatccaga cgtttgcttt ccatcgagct tccttagcgt tttgcctgcc agatacgagc
    10201 ctgataatcg cgaatagagc gatccgagct aaacataccg cagcgggcgg tattgaggat
    10261 cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca tccacctgct tttgtgcctc
    10321 aacataggct gcgaaatccg ccatcaccag atacggatcg ccgccttgtt tgccgatact
    10381 gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg tatttaccgc tttccagctc
    10441 tttcaatact gcgtccagca ccttatcttt cttccgccat ttcaccgggt cgtagccttt
    10501 ggccagaatt gccttcactt gttccacggt atgaccaaaa ataaagatat tttcttcacc
    10561 gactttctcg gagatttcaa cgttcgcacc atccagcgta ccgacagtaa gcgcaccatt
    10621 gagcgccagt ttcatattgc cggtaccgga agcttcttta cctgcagtcg aaatttgttc
    10681 ggagatatcc gccgccggga tcagtttttc cgccgccgaa acgcaataat cctgcaggaa
    10741 caccaccttc aacttatcgc caaccagtgg atcgttgttg atcacgtcag ccactttgtt
    10801 gatcgcaaag ataatattct tcgccaggta gtagcccggt gccgctttcg cgccgaagag
    10861 gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga atttctttgt acaacgccag
    10921 aatatgcagc agattcaggt gctggcgttt gtactcatgc agacgtttaa tctgaatatc
    10981 gaaaatcgcc tgtggattga tctcaatacc ggtacgaact ttcacaaact ccgccagacg
    11041 gactttattc gcctgcttga tctcgcgata ttgctgacgg aatttcgcat catcagcgaa
    11101 tttttccaga ttgatcagct gatcgagatc gttagcccac tctttttgca gtaatttatc
    11161 caacagagcc gccagtgcag ggttgcactg tttgatccag cgacgtgggg taataccgtt
    11221 ggtgacgtta tggaatttgt tcggccatag ctggtgatat tccgggaaca gatctttcac
    11281 caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg aaaccgccaa ccacacacag
    11341 gttcgccata tgcacttgtt tatcgtgcac caccgccagt ttggcccaca ctttttcatc
    11401 gcccggccag gttttctcca ccagcgtttt aaagcgagta ttaatttcgt taataatctg
    11461 catatggcgc ggcagtaagc ctttcaccag tttcacatcc cagcgttcca gcgcttctgg
    11521 catcagggta tggttggtgt aagcgaaagt tttgctggta atggcccagg cgtcatccca
    11581 gctcatctgg tgctcatcga tcagcacgcg cagcagttct ggaatcgcga tagttgggtg
    11641 ggtatcgttc agctgaataa cttcataatc cgccagttcg tgcagtttac gccccgccag
    11701 atgatggcga cgcaaaatat ccgctaccga acaggcacac tggaagtact gctgcatcaa
    11761 gcgcagcttt ttaccggcag tatggttgtc gtttggatag agaactttgg tcagtttttc
    11821 cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg tcgttaaatt tagtcagatc
    11881 aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc tgcgccacgc cattacgata
    11941 gccgacaacg gggagatccc acgcttgacc ggtaatggta aactccggct cccagcgtcc
    12001 gtctttcgtc actttaccgc caatccctac ctgcacatcc agtgcttcgt tgtggcggaa
    12061 ccacgggtag ttactacgat gccagtcatc cggcgcttca acctgtttgc catcgacaaa
    12121 agactggcgg aacaaaccat attgatagtt cagaccgtaa cccgtcgcag actgaccgat
    12181 agttgccatt gagtcgagga agcacgccgc cagacgtccc agaccaccgt tacccagcgc
    12241 cgggtcgatc tcttcttcca gcaggtccgt cagattgatg tcataagcct tcaacgaatc
    12301 ctgtacttcc tgataccagc cgagattcaa caggttgttg cccgtcaggc gaccaatcaa
    12361 aaactccatt gagatgtagt taacatgtcg ctgattcgcc accggcttgg cgaatggctg
    12421 agcacgcagc atttcggcca gtgcttcact cactgctagc caccactggc gaggagtcat
    12481 ttcagccgca gaatttaagc cataacgctg ccactgacgt gaaagcgctt cctgaaattg
    12541 cttatcgtta aaaataggtt gtgacatagg agttccactt ttcttagatt ttcaacacaa
    12601 cgttatcgct agtttgccag gctcgatgtt gaccttcctc atcctgcggg ggattaggca
    12661 gggaggagtt gcggggatga gcaaggaaat gtgatctcaa ccacttaaag ctagtgcaaa
    12721 ccacaggatt agcagcaaat caatgcaata cagcgcagaa aatctgtatc taagtgcaaa
    12781 aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc tataaattaa gtaaaggagt
    12841 gaaacagttt cacaagtaaa atatccagtg tgctccatct cattcttaat agatttatta
    12901 agatcatctt tttagatggc actttcatca ggaatgaaga agaaaccctt gcttaaatga
    12961 atctgatgaa cataagggaa accagtattc acgctggatc agcgtcgttt taggtgagtt
    13021 gttaataaag atttggaatt gtgacacagt gcaaattcag acacataaaa aaacgtcatc
    13081 gcttgcatta gaaaggtttc tggccgacct tataaccatt aattacgaag cgcaaaaaaa
    13141 ataatatttc ctcattttcc acagtgaagt gattaactat gctgattccg tcaaaactaa
    13201 gtcgtccggt tcgactcgac cataccgtgg ttcgtgagcg cctgctggct aaactttccg
    13261 gcgcgaacaa cttccggctg gcgctgatca cgagtcctgc gggctacgga aagaccaccc
    13321 tcatttccca gtgggcggca ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg
    13381 ataaccagca agagcgtttc gccagctatc tcattgccac cgtgcagcag gcaaccaacg
    13441 gtcactgtgc gatatgtgag acgatggcgc aaaaacggca atatgccagc ctgacgtcac
    13501 tcttcgccca gcttttcatt gagctggcgg aatggcatag cccactttat ctggtcatcg
    13561 atgactatca tctgatcact aatccagtga tccacgagtc aatgcgcttc tttattcgcc
    13621 atcaaccaga aaatctcacc ctggtggtgt tgtcacgcaa ccttccgcaa ctgggcattg
    13681 ccaatctgcg tgttcgtgac caactgctgg aaattggcag tcagcaactg gcatttaccc
    13741 atcaggaagc gaagcagttt tttgattgcc gtctgtcatc gccgattgaa gccgcagaaa
    13801 gcagtcggat ttgcgatgac gtttccggtt gggcgacggc actacagcta atcgccctct
    13861 ccgcccggca gaatacccac tcagcccata agtcggcacg ccgcctggcg ggaatcaatg
    13921 ccagccatct ttcggattat ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc
    13981 gccattttct gttgaaaagc gccattttgc gctcaatgaa cgatgcgctc atcacccgtg
    14041 tgaccggcga agaaaacggg caaatgcgtc tcgaagagat tgagcgtcag gggctgtttt
    14101 tacagcggat ggatgatacc ggcgagtggt tctgctatca cccgctgttt ggcaacttcc
    14161 tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc agtgggaact ggcggcggag
    14221 ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt tcccagcgaa
    14281 gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat tctgcttaat
    14341 cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc gcttaaggcc
    14401 ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc gtggctaatg
    14461 caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga acatgaaatc
    14521 aaggacatca gagaaggcac catgcacgca gaatttaacg ctctgcgcgc ccaggtggcg
    14581 attaacgatg gtaatccgga tgaagcggaa cggctggcaa aactggcact ggaagagctg
    14641 ccaccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga agtgctgcac
    14701 tgcaaaggcg aattgacccg ctcactggcg ctaatgcagc aaaccgaaca gatggcacgc
    14761 cagcacgacg tctggcacta cgccttatgg agtttaatcc agcaaagtga aattctgttt
    14821 gcccaagggt tcctgcaaac cgcgtgggaa acacaggaaa aagcgttcca gctgatcaac
    14881 gagcagcatc tggaacagct gccgatgcat gagtttctgg tgcgcatccg agcacaactg
    14941 ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg gattgaagtc
    15001 ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat tcaatgctcg
    15061 ctggcccgtg gtgatttaga taacgcccgt agccagctga accgtctgga aaacctgctg
    15121 gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg ggtgatttac
    15181 tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac ggctaaaccg
    15241 gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg cgcacaaatc
    15301 ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga aaatgcccgc
    15361 agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca actgtactgg
    15421 caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa actggcgaat
    15481 cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca acaactgcgc
    15541 cagctgattc aactcaatac gctgccggaa ctggaacagc atcgcgcgca gcgtattctg
    15601 cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa tttcgttgaa
    15661 cgtctgctaa atcatccgga agtgccagaa ctgatccgca ccagcccgtt gacccaacgt
    15721 gaatggcagg tcctggggct gatctactcc ggttacagca atgagcaaat tgcgggcgag
    15781 ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca aaaactcggc
    15841 gtcgcccacc gtcaggccgc ggtacaacac gcgcagaaac tgctgaagat gatgggatat
    15901 ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac atcattcaat
    15961 actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca agaaacgctc
    16021 caccacggcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa attctcccgc
    16081 gcccgccagc gccatcggta gcaccagctg gtcggcgaga tattccccca ctgccgccgg
    16141 gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct cggcactgac
    16201 gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga cttccagcga
    16261 gacggtatta cccggcccct gatcgcgcgg caggttatga atattctgtt catgcaggga
    16321 aaaactcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca caccagctaa
    16381 tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca aggtgttaaa
    16441 cgatgccacg ggcgagactt ccgttgccac cacgccgcct ccggcaggat aaaaaccgtg
    16501 acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct ccagcacccg
    16561 gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg cctccgctca cttcaacacg
    16621 cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca gcgtacagct
    16681 tccggcacta ccgatagcaa agcggtactc gccgccgcgc acggcgccag gccggaagac
    16741 cagacgctgc gaccccagct ccgcgccttc caccgttgcc ctacaaattt ccgcagccgc
    16801 ttttaccgcg gtcagatgct ggcgcaacag ccccggtttc gcccgcccgg cacgaatgct
    16861 ggtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc gcagaatctg
    16921 cccgccacct tcgccctgtg cgccatccag cgcaatcatc cttttcatca tttatccttt
    16981 tacgcacacc acctgacgca gggtatagat aacttccacc agatcgcttt gtgccgtcat
    17041 caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg catctttacg
    17101 gcattccaca tgcgcggtgg cacgaatttg atcttccacg ctgaacagtt ttttcgcttt
    17161 agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact cttcatttcc
    17221 cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc catattgacc
    17281 agcacgcgca gacaccgcgc ctttacgcgt cacgtagatc tcttcaccaa agtgctgttc
    17341 tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt gtggctgtct
    17401 gaccgttttc tgcgtaatgc tctgcaatgc cgttaccacg ttttccatca tcacatcgcg
    17461 gttaaggctg gcaaaaagct gcgcccaggc cacggctttc aggtaatcat caaagtattc
    17521 cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagcg tttcctgcat
    17581 ctctttttgt gccagatcga taaagtaagt cccgatggcg ttaccaattc cgcgtgaacc
    17641 ggagtgcagc ataatccaca cctggtccga ctcatcaagg cagattttaa taaagtggtt
    17701 accggttccc agcgttccca ggtgtttata gttattggta ttcaggaaac ggggatattt
    17761 ttgcgttaac cactgataac cggcttcaag ctcagcccat ttcgcatcga cgttgacagg
    17821 cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc cgtgcggcac
    17881 ggccgtttca atcgcctgac gcagctctgc caggttttca ggcaggtctt ccgccgttaa
    17941 cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg ccggaataat
    18001 cgcccctttg gtcgggatca cgctaccaat ggtggaatct ttacccaggt gtacatcagg
    18061 cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa gttgctgacg
    18121 cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat tttcagtggt
    18181 cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgctgtcct gataattgca
    18241 accgtcgtgc cagaaaatta aataagtaaa ttaatttttt aattcattgt ttttaaaaag
    18301 attatatctt tatgtccgta accggagatt tcccgcaaag ccgatttacc gataatgaaa
    18361 tatcgtcttt tataaggata tctaagatgc gtaaaacagt agcttttggc tttgtcggaa
    18421 cggtgctgga ttatgccggg cgcggcagtc agcgctggtc aaaatggcat ccgacactct
    18481 gtttatgcca gcaagaatcg ttggtcatcg atcgactgga attattgcat gacgcccgct
    18541 cgtgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca gaaacagaag
    18601 tggtgagcat tgagattgaa ctgcataacc cgtgggattt cgaagaggtt tacgcctgcc
    18661 tgcatgattt cgcccgtggt tacaagtttc agccagaaaa agaagactat ttaattcaca
    18721 tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa gcgcgttatc
    18781 tacccgcccg gttgatacaa acttcaccac cgcgcaaaaa agaacaaccg cgcggtgccg
    18841 gtgaagtgac gattatcgat ctcgatctaa gccgttataa cgccatcgcc agccgctttg
    18901 ccgaggaacg ccagcaaacg cttgattttc ttaagtccgg cattgccacg cgtaaccccc
    18961 acttcaaccg catgattgag cagattgaaa aagtggcgat taaatcccgc gcaccgatcc
    19021 tgcttaacgg cccaaccggt gcgggaaagt catttctggc gcgacgtata tttgaattaa
    19081 aacaggcgcg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc accctgcgcg
    19141 gcgataccgc catgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc ggggcgcggg
    19201 aatctcgtga aggtttatta cgcagcgcca acggcggaat gttgtttttt gatgagattg
    19261 gcgaactggg cgcagacgaa caggcaatgc tgctaaaagc cattgaagag aaaacctttt
    19321 acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc ggaacggtgc
    19381 gcgatttgcg ccagctggtt gccgaaggca aatttcgcga agatctgtac gcgcggatca
    19441 atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt gaaccgaacc
    19501 ttgattatga agtggagcgc cacgccacac tcactggcga cagcgtgcgt tttaacaccg
    19561 aagcgcggcg cgcctggttg gcttttgcga cctctcccca ggcaacatgg cgcggtaact
    19621 ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc ggacgcatca
    19681 ctctggacgt ggttgaagat gagataaacc gtctgcgcta taactggcag gagagccgcc
    19741 cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc ttcgaccgta
    19801 tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct gcagccggac
    19861 gacagctttt tgatgtttcg cgccagggaa aagccagcgt caatgacgcg gatcggctac
    19921 gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag cacagctcca
    19981 gttgaatatg atggtccgtc agcacctgca ttacgcttac tggcggcggg gcgtcggtgt
    20041 agacggcatc taccatgctg atgctgccca tattgaccat cgcgttacgg ccaaatttcg
    20101 agtgatcgac aaccagcata acgtggcgcg agttctcaat aatggcgcgc ttggtgcgaa
    20161 cttcgtggta atcgaactcc agcagcgagc cgtcgctatc gatgccgctt atccccagaa
    20221 tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca atgatcccgc
    20281 catcgcggct gcgtaattcg ccaccagcga gaataatgcg gaaatcttct tttaccatca
    20341 acgtgttagc aacattgaga ttgttggtga caatacgcaa attgctgtga ttaagcagcg
    20401 cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagtgtcgag ccgttgggga
    20461 tttgctccgc cactttgcgg gcaatcctct ctttttcttc ggtctgggtg gctttgcgat
    20521 cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc accgccatgg tgacgcagga
    20581 tcaggttttg ctccgccagc tcattgaggt cgcggcgaat agtctgcggg ctgacggaga
    20641 aatgctctac cagctcttca gtactgacat aaccctgctg tttaaccagt tcgataatac
    20701 cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg ttttcgcgca
    20761 ttgagcgaat caacaaaagc catcgctaaa cccacggcta acccggcgat gtgtgctccg
    20821 ttcgccatcg acatcccaaa caaatcaaac catccggcga taatccagat cagcgcaaag
    20881 ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgctcacc acgtagccag
    20941 acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg cccgctgaat
    21001 ttttgctgca catagccgct taacagggca ctaatgagag taataacaat tagcttaccg
    21061 ctaccgaggc gtttttccac cgcaccgccg agataccacc accagagcag gttaaagagg
    21121 atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa ctcaaatttc
    21181 agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc gagaatttgc
    21241 atggcgataa acaccaccac gcaggcgatc atcaccaccc aggttaccgg acctgcgcgt
    21301 tcacgcaagg cagcaaagaa aggataacgg cgataatgca gaccactgcc ggtatggccc
    21361 gcctgccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa acgcgccagc
    21421 tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct ttggttatgt
    21481 tgttgaatcg taaggataac accctgcgtc gccatgtaat caacaaacgc ctgcgccacg
    21541 cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc cacacaaaag
    21601 gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactga cgctgccagg
    21661 cttcaaaacc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc agatactgcg
    21721 ccgcgccttt gctgctattg ccgtgataac acatcaccat caccggagtg tcaaagtcgt
    21781 tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc accgcatgtc
    21841 ccattgcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt tcctgcaact
    21901 tctggtgcgc gtcggcaacg ttaatacatt cgaacagatc catgcgtctc tctttcttta
    21961 caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa acgccataat
    22021 gttatacata tcactctaaa atgttttttc aatgttacct aaagcgcgat tctttgctaa
    22081 tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca gcatggaaac
    22141 caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg cagacgccgc
    22201 tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg cgacctcttc
    22261 cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg aattccgcct
    22321 ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc atatcgcctt
    22381 cccaatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga tgattcggat
    22441 tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat caaccggttt
    22501 gcgttttggc gcaaactcag tgttaaaacc ggaaattaag cgcggattcg aatattctga
    22561 ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg tgcgtaaagg
    22621 cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg gcctgtggat
    22681 tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc gcggcctggt
    22741 taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc tgccttcgcc
    22801 ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgtgtgc atacccagaa
    22861 gcaggcctac attctgcaaa acgaagataa acgtattgtg ttcgtgatcc cgtggatgga
    22921 cgaattttcc atcatcggca ccaccgacgt agagtataaa ggcgatccga aagcggtgaa
    22981 gattgaagag agtgaaatca actatctgct gaaagtgtat aacgcgcact ttaaaaaaca
    23041 gcttagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt gtgatgatga
    23101 gtccgactcg ccgcaggcta ttacccgtga ttacaccctt gatatccatg atgaaaacgg
    23161 caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa aactggcaga
    23221 acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt ggacgaaaga
    23281 gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgcag ctcgtctgcg
    23341 ccgtcgctat ccattcctga ctgaatcgct ggcgcgtcat tacgcacgca cttacggcag
    23401 caacagcgag ctgctgctcg gcaatgcggg ggcgataagc gacctcgggg aggatttcgg
    23461 tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg tccgccgcac
    23521 cgacgatgcc ctttggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg atcaacaatc
    23581 tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg cgtcgtaagg
    23641 atgatgcccg gttgaagtgc aaccgggcta tttggattcc ttagactatt cattgtaaat
    23701 aattgaataa cggcccctga gctaaaatcg ccaaacttaa gcacgttacc caatcgcccc
    23761 acttcttcct ctggtttcta ttttttctgc accaatggca ataatatccc aatgcaaaac
    23821 caagctgtgc actaatcggg ccgacaaaat cagtccaagg ctgcggccaa aaaggggaaa
    23881 aattcagggg aggccagaca aacgtcagaa agaaatttcg gatatttccc agcaacgctg
    23941 atttctctgg agaaatataa agaatgaaat tcgagagaat taaccagaaa tagaccggcg
    24001 aaaagaccag ccagcaatgc agtctgtttt ttaattcagg aggcgttctg cgggagaatt
    24061 gccaaccgat gccagccatt acac
//
LOCUS       DAJVOY010000050        23237 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVOY010000050 DAJVOY010000000
VERSION     DAJVOY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.050"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..564)
                     /locus_tag="OQL90_001747"
     CDS             complement(<1..564)
                     /locus_tag="OQL90_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1368558.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGH"
     gene            complement(783..1760)
                     /gene="epmA"
                     /locus_tag="OQL90_001748"
     CDS             complement(783..1760)
                     /gene="epmA"
                     /locus_tag="OQL90_001748"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1368559.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2085..3893
                     /gene="frdA"
                     /locus_tag="OQL90_001749"
     CDS             2085..3893
                     /gene="frdA"
                     /locus_tag="OQL90_001749"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1368560.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3886..4620
                     /gene="frdB"
                     /locus_tag="OQL90_001750"
     CDS             3886..4620
                     /gene="frdB"
                     /locus_tag="OQL90_001750"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1368561.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4631..5026
                     /gene="frdC"
                     /locus_tag="OQL90_001751"
     CDS             4631..5026
                     /gene="frdC"
                     /locus_tag="OQL90_001751"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1368562.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5037..5396
                     /gene="frdD"
                     /locus_tag="OQL90_001752"
     CDS             5037..5396
                     /gene="frdD"
                     /locus_tag="OQL90_001752"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1368563.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5459..6592
                     /locus_tag="OQL90_001753"
     CDS             5459..6592
                     /locus_tag="OQL90_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1368564.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6681..7214
                     /gene="blc"
                     /locus_tag="OQL90_001754"
     CDS             6681..7214
                     /gene="blc"
                     /locus_tag="OQL90_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1368565.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7211..7528)
                     /gene="sugE"
                     /locus_tag="OQL90_001755"
     CDS             complement(7211..7528)
                     /gene="sugE"
                     /locus_tag="OQL90_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1368566.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7710..7856)
                     /gene="ecnB"
                     /locus_tag="OQL90_001756"
     CDS             complement(7710..7856)
                     /gene="ecnB"
                     /locus_tag="OQL90_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1368567.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7967..8092)
                     /gene="ecnA"
                     /locus_tag="OQL90_001757"
     CDS             complement(7967..8092)
                     /gene="ecnA"
                     /locus_tag="OQL90_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1368568.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8144..8710)
                     /gene="efp"
                     /locus_tag="OQL90_001758"
     CDS             complement(8144..8710)
                     /gene="efp"
                     /locus_tag="OQL90_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1368569.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8752..9801
                     /gene="epmB"
                     /locus_tag="OQL90_001759"
     CDS             8752..9801
                     /gene="epmB"
                     /locus_tag="OQL90_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1368570.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10175..11044
                     /locus_tag="OQL90_001760"
     CDS             10175..11044
                     /locus_tag="OQL90_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCS1368571.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11247..11600)
                     /locus_tag="OQL90_001761"
     CDS             complement(11247..11600)
                     /locus_tag="OQL90_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1368572.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11738..13384)
                     /gene="groL"
                     /locus_tag="OQL90_001762"
     CDS             complement(11738..13384)
                     /gene="groL"
                     /locus_tag="OQL90_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1368573.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13428..13721)
                     /locus_tag="OQL90_001763"
     CDS             complement(13428..13721)
                     /locus_tag="OQL90_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1368574.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13997..15253
                     /gene="yjeH"
                     /locus_tag="OQL90_001764"
     CDS             13997..15253
                     /gene="yjeH"
                     /locus_tag="OQL90_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1368575.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(15269..15745)
                     /gene="fxsA"
                     /locus_tag="OQL90_001765"
     CDS             complement(15269..15745)
                     /gene="fxsA"
                     /locus_tag="OQL90_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1368576.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            16082..17518
                     /gene="aspA"
                     /locus_tag="OQL90_001766"
     CDS             16082..17518
                     /gene="aspA"
                     /locus_tag="OQL90_001766"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1368577.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17636..18937
                     /gene="dcuA"
                     /locus_tag="OQL90_001767"
     CDS             17636..18937
                     /gene="dcuA"
                     /locus_tag="OQL90_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1368578.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            19053..19391
                     /gene="cutA"
                     /locus_tag="OQL90_001768"
     CDS             19053..19391
                     /gene="cutA"
                     /locus_tag="OQL90_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1368579.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19367..21064
                     /gene="dsbD"
                     /locus_tag="OQL90_001769"
     CDS             19367..21064
                     /gene="dsbD"
                     /locus_tag="OQL90_001769"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1368580.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            21101..21676
                     /locus_tag="OQL90_001770"
     CDS             21101..21676
                     /locus_tag="OQL90_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1368581.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21783..21858
                     /locus_tag="OQL90_001771"
     tRNA            21783..21858
                     /locus_tag="OQL90_001771"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21816..21818,aa:Phe,seq:gaa)
     gene            22056..23027
                     /locus_tag="OQL90_001772"
                     /pseudo
     CDS             22056..23027
                     /locus_tag="OQL90_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         5805 a         5992 c         5747 g         5693 t
ORIGIN      
        1 atgcccacca tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca
       61 cattactgca ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc
      121 aacgacggtg accagaatca tccatgctac tgccagcaga ccaaccacct gcgtaggctc
      181 cagtccggca atacgccagt gctgggtcat gtcgctacca tagaggaatg ttgagaacgg
      241 tacccaaact ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa
      301 cgtaccaatg aaggcaaaac gtggtccgac actattattc atccaggaat agataccacc
      361 ttcttctttg cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa
      421 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      481 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      541 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      601 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      661 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      721 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      781 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      841 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      901 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      961 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
     1021 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
     1081 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
     1141 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
     1201 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
     1261 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
     1321 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1381 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1441 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1501 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1561 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1621 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcagc aaagaaacga
     1681 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
     1741 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1801 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1861 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1921 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1981 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     2041 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     2101 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     2161 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     2221 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     2281 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     2341 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2401 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2461 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2521 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2581 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2641 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2701 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2761 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2821 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2881 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2941 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     3001 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     3061 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     3121 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     3181 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     3241 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgta
     3301 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagaggcaa
     3361 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3421 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctagc aatggaagaa ggttgcggta
     3481 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3541 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3601 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3661 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3721 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
     3781 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3841 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3901 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3961 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     4021 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     4081 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     4141 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     4201 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     4261 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     4321 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4381 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4441 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4501 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4561 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4621 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4681 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4741 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggacgcctg
     4801 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4861 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4921 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4981 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     5041 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     5101 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     5161 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     5221 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     5281 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     5341 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5401 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5461 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5521 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5581 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5641 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5701 cagcaaaaca tttacgggcg tgcttggtgg cgatgctatt gctcgagggg aaatcaagtt
     5761 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
     5821 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcagatgc cggatgaagt
     5881 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5941 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     6001 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     6061 ccatacgtgg attaatgtac cgcccgcaga agaaaagaat tacgcctggg gatatcgcga
     6121 aggcaaggca gttcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     6181 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     6241 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     6301 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6361 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6421 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6481 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6541 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6601 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6661 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6721 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagtg gtgaataatt
     6781 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6841 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatggcggc ggcctgaatg
     6901 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6961 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     7021 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     7081 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     7141 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     7201 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     7261 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatagccg
     7321 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctactggt aacgatttca
     7381 tcgcccaggc aagtagcgcc atactgacaa tcatcgccgt cacggtaata acactcggcg
     7441 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7501 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
     7561 tgaaaagacg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7621 gtggcagggg aatttcaact attaccttag tgactatcgc agaaaaaaca aacggcacga
     7681 cacagctgtc gtaccgttat tgcttattgc tattgctgcg ctttcgttgc tgcgccagaa
     7741 atcgcgttac cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt
     7801 aatactgttg aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg
     7861 tcttcctttt aggtaatgtt atttatgttt gcctatagca aattaataat agacaacatc
     7921 gccaaaaatg acggaaatct gatgattttt cggaagagaa gaaaaattag ctggcagcgc
     7981 gagagatgga gttgccgaga tgtttgatgt cttcgccgaa accgcgagcg gtgttacagc
     8041 ccgtgagcag cgtactggca agcaaaacaa gaacgataag gcgtttcatc atctctgacc
     8101 tgaataagtg atggtgcagc ctgcaggccg caccacaacc gcattacttc acgcgagaga
     8161 cgtattcacc agagcgggta tccactttga tgacttcgcc gatttgtacg aacagcggaa
     8221 ccttaaccac agcgccagta gacagggtag ccggtttgcc gccagtacct gcggtatcac
     8281 ctttcaggcc cggatcggta tcaacgattt ccagttcaac gaagttcggc ggagtaacgg
     8341 agatcggctg accattccac agagttacga tacactctgc ctgatccagc agccatttag
     8401 cgttatcgcc aattgctttt gcatcagcag acagctgctc gaaagtttcg ttgttcatga
     8461 agtgccagaa ctcaccgtcg ttgtacaggt aagtcaggtt catatcgaca acatcagcgc
     8521 cttcagcgga atcagtagat ttgaaggttt tttctacgcg agtaccggtc agcagacgac
     8581 gcagtttaac gcgagcaaat gcctggcctt tacccggttt tacgaattca ctcgcttcaa
     8641 ccgcgtaagg ttcgccgtct aacatgattt taagaccagc acgaaaatcg ttgctatagt
     8701 acgttgccat aaggccctct gaaatttgtt aattggtagc taagccacaa aatggcgcat
     8761 attgtaaccc taaatacccc atccagagaa gattggttaa cgcaacttgc cgatgttgtg
     8821 accgatcctg atgaacttct gcgtcttttg aatatagacg cggacgaaaa actgttggcc
     8881 ggacgcagcg ccaaaaagct gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg
     8941 gagaaaggca atccggacga tcctcttttg cgtcaggtac ttacctcgca agatgagttt
     9001 gtcgtcgcgt ccggattctc caccgaccca ctggaagaac agcacagcgt agtgcctggt
     9061 ttgttgcata aataccacaa ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat
     9121 tgccgctatt gcttccgtcg ccacttcccc tatgccgaaa atcagggcaa caagcgtaac
     9181 tggcagactg cacttgagta tgttgctgcg catccggaac tggacgagat gattttctcc
     9241 ggcggcgatc cgctgatggc gaaagatcac gagctggact ggttgctcac acaactggaa
     9301 gccatcccgc atatcaaacg tctgcggatt cacagccgtc tgccgattgt gatcccggca
     9361 cgcatcacag atgcgctggt tgaacgcttt tcacattcta cgctgcaaat cctgctggtg
     9421 aatcacatca accatgccaa tgagatagat gaaacattcc gtcaggcgat ggctaagttg
     9481 cgccgggtag gcgttacttt gctgaaccag agcgttctgt tacgtgatgt gaacgataac
     9541 gcacaaacgc tggcaaacct gagtaatgcg ttgttcgatg ccggcgtgat gccgtattac
     9601 ctgcatgtgc tcgataaagt acagggcgca gcgcatttta tggtgagtga tgacgaagca
     9661 cggcagatta tgcgtgagtt gctgacattg gtgtcgggat atctggtgcc gaaactggcg
     9721 cgagaaattg gcggcgaacc cagcaaaacg ccgctggatc tccagctacg ccagcagtta
     9781 ctgattaaac agggaagtta aacattaaaa ttgcctgatg cgctacgctt atcaggccta
     9841 catcgtgcat tgaaattgaa tggatttgca atgaactgta ggccggataa ggcgtttacg
     9901 ccgcatccgg caagagcaac aaacgcaagt acagcaacct gtcattaaac tgagaaattt
     9961 aatctttgcc cgttgcataa cctttaatta ttcatatgca ttgctgttgt tactttttat
    10021 atagctaaca ccaataatta acctttcttt tctgtttata tagagtgagc acggctattt
    10081 atgttatttt aacaactatt actaccaagc ccatttatct ggcttataaa ttaaaattaa
    10141 ttatttaatt gagaaatggt tagggagaac ctacatggcc ataagcatca agggtgttaa
    10201 taccggtgtt attcgcaaga gtaataattt cattgcactg gcgctgaaaa ttaaagaacc
    10261 tcgcaataaa gaatcgttgt tctttatgtc tgtcatggaa ctgcgcgacc tgctgattgc
    10321 gctggaaagt cgtctgcatc aaaagcataa actggatgct gctgcacgcc ttcagtatga
    10381 gcaagctcgc gataaagtca ttaagaaaat ggcagaaaac atcccagaaa ttctggttga
    10441 tgagcttaaa aatgccgata ttaatcgtcg ggttaacacg ctggaactta ccgataatca
    10501 aggcgaaaat cttaccttcg tattaacgct acatgacggt agcaaatgtg agttagtcgt
    10561 caatgagttg cagattgaaa tgctagcgcg agcaatcatt catgcaatta ataatgcgga
    10621 aatgcgtgaa ctggcattac gtattacctc actgttagat ttcctgccgt tatatgacgt
    10681 tgactgccag gaaaatggca atctggaata tgacacctat tcccaaccgg aatggaaaca
    10741 taacttgttc gaccattatc tggcggtgct ttaccgcttt aaagatgaaa gtggcaaaga
    10801 acaatttagc ggcgcagtgg tgaaaacgcg tgaagcaacg ccgggtaaag aagtagaggc
    10861 gatcactcgc cgaatgcttg atttcagccc acgactgaaa aaactcgcgg gtgtgccatg
    10921 tcaggtctat gttcgcaccg tagcggcgaa taacgcccaa ccgctaaccc aggatcaatg
    10981 cttacgcgcg ctgcatcact tacgtgttca atccaccagc aaaactgcac cacaggcgaa
    11041 ataatctcat ctttgctaat gcgaggctgg agccatccag cctgaaatga ctgataacgt
    11101 gatcgttggt catgccggat gcggcgtaaa cgccttatcc ggcctacaaa ttctcaagca
    11161 ttcaatcagt tgcaatgcct gctgtaggcc tgataagcga agcgcatcag gcaataatac
    11221 gtttgtcacc cagcctctgg atcacatcag ttcgggcact tataaacctg accgataatc
    11281 tggctatccg tcgggacaaa actggacaac attccctgcg acgggctgct gatgccataa
    11341 atcacgttac cgcccattgc agccgcctgg ttgcgcagat cgtttgctgc gccgcgcata
    11401 gaaccgccct cttctccgtg ttgcccggaa agccagttgc tttgcttacc tgtcgcagta
    11461 ccaatcagct ggcactctgc gcctggctgc tcgtccacaa tgcgtacact ctgaccggca
    11521 gcactcaatt cgttgctgga gctacaaccc gccatcagta gcgcggcacc gacaatccct
    11581 gctaagtatt ttacgtgcat gttattcccc ataatcaatg agctggacgc cactcgcgtc
    11641 gtccgtgtct gaatcttata ctagaaagat gaccaaaaga aaaaccccca gacatttctg
    11701 cccgggggtt tgtttatttc tgcgaggtgc agggcaatta catcatgccg cccatgccac
    11761 ccatgccgcc cataccgcca gcagcgccta agtcagctgc atcgtttttc ggcaggtcgg
    11821 taaccatgca ctcggtggtg atcatcaggc cagccacaga agccgcgtac tgcagagcag
    11881 aacgggttac tttggttgga tccaagatac ccatgtcgat catgttgccg tattcttcgg
    11941 ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac agtgttagca acaacagacg
    12001 gttcttcgcc gcagttcaat acgatctgac gcagcggagc ttccattgca cgcagtgcaa
    12061 ctttgatacc cacgttctgg tcttcgttct gaccacgcag gtcagccagt ttagacgcta
    12121 cgcggatcag cgcaacacca ccaccagcaa ccacgccttc ttctaccgca gcacgggtcg
    12181 cgtgcagggc atcttcaacg cgtgcttttt tctctttcat ttcaacttcg gtagcagcac
    12241 ctactttgat aactgcaacg ccgcctgcca gtttcgctac gcgctcctgc agtttttcac
    12301 ggtcgtagtc agaagttgct tcttcaatct gctgacggat ctgagcaaca cggccctgga
    12361 ttgcagcttc ttcacccacg ccatcaatga tggtggtggt gtctttgttg atcacaacac
    12421 gtttagcctg acccaggtct tccagggttg ctttttccag ctccataccg atctcttcag
    12481 agattacggt accgccagtc agggttgcga tatcctgcag catagcttta cggcgatcgc
    12541 cgaagcccgg agctttaacc gcagcaactt tcacgatgcc acgcatggtg ttaacaacca
    12601 gagttgccag cgcttcgcct tcaacatctt cagcgatgat cagcagcggt ttacctgctt
    12661 tagcaacggc ttccagaacc ggcagcattt cgcggatgtt ggagattttc ttgtcagcca
    12721 gcaggatgaa cgggctttcc agttctactg cgccagtttc cggcttgttg atgaagtaag
    12781 gagacaggta gccacggtcg aactgcatac cttcaaccac gtccagttcg tcctgcagac
    12841 cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac tttgtccatc gcttcagcga
    12901 tcagtttacc tacggtttcg tcggagttag cggagatggt accaacctga gcaatcgctt
    12961 tagagtcaga gcatggtacg gacagcgctt tcagttcttc aactgcagcg gtaacagctt
    13021 tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc agcaactgct ttcagaccct
    13081 cagtgatgat agcctgagcc agtacggttg cagtggtggt accgtcgcct gcagcgtcgt
    13141 tcgctttaga ggcaacttct ttcaccatct gcgcacccat attttcgaac ttgtcttcca
    13201 gttcgatttc acgagcaacg gaaacaccat ctttggtgat ggtcggtgca ccgaaagatt
    13261 tatccagaac tacgttacgg cctttcggac cgagggtaac tttcactgca tctgccagta
    13321 cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc gaattttacg tctttagctg
    13381 ccattatctt tattccttaa attcgtatgt tcagtgtcgt gcgaggatta cgcttcaaca
    13441 attgccagaa tgtcgctttc ggacatgatc aacacttctt cattgtcgat cttctcagat
    13501 ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa ctttcacatc cagcggcttc
    13561 acttcgccat tttcaaggat acggccattg ccgacagcca gcacttcgcc gcgggtggat
    13621 ttagccgctg cagagccggt cagaacgatg ccgccagcag atttagtttc aacttcttta
    13681 cgcttgacga tcacgcgatc atgcaatgga cgaatattca ttgataactc tcctttgaga
    13741 aagtccgtat ctgttatggg tgacgccgga gcttacgtgg tttcccggct ggtgaccaga
    13801 gaaatgggga tgaggattcg ccccttcaag ggggaaaaag aaaaaaaatt ctgatcagca
    13861 caaaatcggg tgaaaaccct gattcacctc acatttcatc gcaatttctt catcgccgtc
    13921 ataagcgaat ctgattgtgc taccatcgaa aatctacgca gttgcccaaa atttgggcgc
    13981 aatcggatat caacccatga gtggactcaa acaagaactg gggctggccc agggcattgg
    14041 cctgctatcg acgtcattat taggcaccgg cgtgtttgcc gttcctgcgt tagctgcaca
    14101 ggtagcaggc aataacagcc tgtgggcgtg gcccgttttg attatcttag tgttcccgat
    14161 tgcgattgtg tttgcgattc tgggtcgcca ctatccctgc gcaggcggtg tcgcgcactt
    14221 cgtcggtatg gcgtttggtt cgcggcttga gcgagtcacc ggctggttgt ttttatcggt
    14281 cattcccgtg ggtttgccag ccgcgctaca aattgccgcc gggttcggcc aggcaatgtt
    14341 tggctggcat agctggcaac tgttgttggc agaactcggt acgctggcgc tggtgtggta
    14401 tatcggtact cgcagtgcca gttccagtgc taatctacaa accgttattg ccggacttat
    14461 cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc aaacctgcga atatcccctt
    14521 cccggcacct ggtaatatcg aacttaccgg gttatttgct gcgttatcag tgatgttctg
    14581 gtgttttgtc ggtctggaag catttgccca tcttgcctcg gaatttaaaa atccagagcg
    14641 tgattttcct cgcgcattga tgattggtct gctgctggca ggattagtct actggggctg
    14701 tacggtagtc gtcttacact tcgacgccta tggtgaaaaa atggcggcgg cagcatcgct
    14761 tccaaaaatt gtagtgcaac tgttcggtgt aggagcgtta tggattgcct gcgtgattgg
    14821 ctatctggcc tgctttgcca gtctcaacat ttatatacag agcttcgccc gcctggtctg
    14881 gtcgcaggcg caacataatc ctgaccacta cctggcacgc ctctcttctc gccatatccc
    14941 gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg agcactttgg tgattcatgc
    15001 tttagagatc aatctggacg ctcttattat ttatgccaat ggcatcttta ttatgattta
    15061 tctgttatgc atgctggcag gctgtaaatt attgcaagga cgttatcgac tactggcggt
    15121 ggttggcggg ctgttatgcg ttctgttact ggcaatggtc ggctggaaaa gtctctatgc
    15181 gctgatcgtg ctggcggggt tatggctgtt gctgccaaaa cgaaaaacgc cggaaaatgg
    15241 cataatcaca taatccggcg tttcgacatt aatcctggcg atcgtcttta tgatcaaggc
    15301 ggtcgcgctc atcatccttt cgctggtact caccatcaaa agtattaccg ccaccggtcc
    15361 cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa atgcggcatc aacttcactg
    15421 tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc gaggaagtcg gtgaaaaagc
    15481 ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac acttttaatc atctccgccg
    15541 ctgggttttc acccgccgcc attttttgct gcatcagcac gaaattctta aagccctggt
    15601 tacgtaccag tgacatcccg ataactgacg tgaatataac cagcacgagg gtcagcaata
    15661 cccccaatac atgggcaacc tgaataaaga ttgaaatctc aatatagaca taaaggaaaa
    15721 tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa tagcagccgg taaaacccgg
    15781 ctacctgaat gggttgcgaa tcgcgttcag cttatattgt ggtcattagc aaaatttcaa
    15841 gatgtttgcg caactatttt tggtagtaat cccaaagcgg tgatctattt cacaaattaa
    15901 taattaaggg gtaaaaaccg acacttaaag tgatccagat tacggtagaa atcctcaagc
    15961 agcatatgat ctcgggtatt cggtcgatgc aggggataat cgtcggtcga aaaacattcg
    16021 aaaccacata tattctgtgt gtttaaagca aatcattggc agcttgaaaa agaaggttca
    16081 catgtcaaac aacattcgta tcgaagaaga tctgttgggt accagggaag ttccagctga
    16141 tgcctactat ggtgttcaca ctctgagagc gattgaaaac ttctatatca gcaacaacaa
    16201 aatcagtgat attcctgaat ttgttcgcgg tatggtaatg gttaaaaaag ccgcagctat
    16261 ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg aatgccatca ttgccgcatg
    16321 tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc ccggtagacg tctaccaggg
    16381 cggcgcaggt acttccgtaa acatgaacac caacgaagtg ctggccaata tcggtctgga
    16441 actgatgggt caccagaaag gtgaatatca gtacctgaac ccgaacgacc atgttaacaa
    16501 atgtcagtcc actaacgacg cctacccgac cggtttccgt atcgcagttt actcttccct
    16561 gattaagctg gtagatgcga ttaaccaact gcgtgaaggc tttgaacgta aagctgtcga
    16621 attccaggac atcctgaaaa tgggtcgtac ccagctgcag gacgcagtac cgatgaccct
    16681 cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa gaagtgaaaa atatccaacg
    16741 taccgctgaa ctgctgctgg aagttaacct tggcgcaaca gcaatcggta ctggtctgaa
    16801 cacgccgaaa gagtactctc cgctggcagt gaaaaaactg gctgaagtca ctggcttccc
    16861 atgcgtaccg gctgaagacc tgatcgaagc gacctctgac tgcggcgctt atgttatggt
    16921 tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa atctgtaacg acctgcgctt
    16981 gctctcttct ggcccacgtg ccggcctgaa cgagatcaac ctgccggaac tgcaggcggg
    17041 ctcttccatc atgccagcta aagtaaaccc ggttgttccg gaagtggtta accaggtatg
    17101 cttcaaagtc atcggtaacg acaccactgt taccatggca gcagaagcag gtcagctgca
    17161 gttgaacgtt atggagccgg tcattggcca ggccatgttc gaatccgttc acattctgac
    17221 caacgcttgc tacaacctgc tggaaaaatg cattaacggc atcactgcta acaaagaagt
    17281 gtgcgaaggt tacgtttaca actctatcgg tatcgttact tacctgaacc cgttcatcgg
    17341 tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa accggtaaga gtgtacgtga
    17401 agtcgttctg gaacgcggtc tgttgactga agcggaactt gacgatattt tctccgtaca
    17461 gaatctgatg cacccggctt acaaagcaaa acgctatact gatgaaagcg aacagtaatc
    17521 gtacagggta gtacaaataa agaaggcacg tcagatgacg tgcctttttt cttgtgaaca
    17581 gtaacttaaa aacaacaatc taatatcaac ttgttaaaaa acaaggaagg ctaatatgct
    17641 agttgtagaa ctcatcatag ttttgctggc gatcttcttg ggcgccagat tggggggaat
    17701 aggtattggt tttgcaggcg gattgggggt gctggttctt gccgctattg gcgttaaacc
    17761 cggtaacatc ccgttcgatg tcatttccat tatcatggcg gttatcgccg ctatttctgc
    17821 catgcaggtt gctggcggtc tggactatct ggttcatcag acagaaaagc tgctgcgccg
    17881 taacccgaaa tacatcacga tcctcgcacc tatcgtgacc tatttcctga caatctttgc
    17941 tggtactggc aacatctctc tggcgacact gccagttatc gctgaagttg cgaaggaaca
    18001 aggcgttaaa ccttgccgtc cgctctctac tgcagtggta tccgcgcaga ttgcgatcac
    18061 cgcatcgcca atctcagcgg cagtggttta catgtcttcc gtgatggaag gtcatggcat
    18121 cagctacctc catctgctct ccgtggtcat cccgtccacc ctgctggcgg ttctggtgat
    18181 gtcctttctg gtcactatgc tgttcaactc caaactctct gacgatccga tttatcgcaa
    18241 gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag cagattgaaa tcaaatccgg
    18301 tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt ggcgtggtta tctatgcaat
    18361 catcaacagc ccaagcatgg gtctggttga aaaaccgctg atgaacacca ccaacgcaat
    18421 cctgatcatc atgctcagcg ttgcaactct gaccaccgtt atctgtaaag tcgataccga
    18481 caacatcctc aactccagca ccttcaaagc aggtatgagc gcctgtattt gtatcctggg
    18541 tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc gactggatca aagataccgc
    18601 tggtgaagtg attcagggtc atccgtggct gctggccgtc atcttcttct ttgcttctgc
    18661 tctgctgtac tctcaggctg caaccgcaaa agcactgatg ccgatggctc tggcactgaa
    18721 cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg tctggtctgt tcattctgcc
    18781 gacctacccg acgctggttg ctgcggtaca gatggatgac acgggtacta cccgtatcgg
    18841 taaattcgtc ttcaaccatc cgttcttcat cccgggtact ctgggtgttg ccctggccgt
    18901 ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga tcaatcgcgg ggcgttcacg
    18961 ccccgctttc tttcccgccg actaacatcc ttcccccgta cgttgtatag tgacctctct
    19021 cttgcggttc catctgttct tgcgaggtgt ttatgcttga tgaaaaaagt tcgaataccg
    19081 cgtctgtcgt ggtgctatgt acggcaccag atgaagcgac agcccaggat ttagctgcca
    19141 aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat ccccggcgct acctctctct
    19201 attactggga aggtaagctg gagcaagaat acgaagtgca gatgatttta aaaactaccg
    19261 tatctcacca gcaggcactg ctggaatgcc tgaagtctca tcatccatat caaaccccgg
    19321 aacttctggt tttacctgtt acacacggag acacagatta cctctcatgg ctcaacgcat
    19381 ctttacgctg atcctgctac tttgcagcac ttccgttttt gccggattat tcgacgcgcc
    19441 gggacgttca caatttgtcc ccgcggatca agcctttact tttgattttc agcaaaacca
    19501 acatgacctt aatctgacct ggcagatcaa agacggttac tacctctacc gtaaacagat
    19561 ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag ctgccgcaag gcgtctggca
    19621 tgaagatgag ttttacggca aaagcgagat ttaccgcgat cggctgacgc ttcccgtaac
    19681 catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc taccagggct gtgctgatgc
    19741 cggtttctgt tatccgccag aaaccaaaac cgttccgtta agcgaagtgg tcgccaacaa
    19801 cgcagcgtca cagcctgtgt ctgtttcgca gcaagagcag cacaccgcgc aattgccctt
    19861 ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc tttacgccat gcgtgctgcc
    19921 aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa cagcggctct ccactgccag
    19981 agcattgttg ctgaccttta tttatgtgca ggggatggca ctgacttaca cggcgctggg
    20041 tctggtagtt gccgccgcag ggttacagtt ccaggcggcg ctacagcacc catacgtgct
    20101 cattggcctc gccatcgtct ttaccttgct ggcgatgtca atgtttggct tgtttactct
    20161 acaactcccc tcttcgctgc aaacacgtct cacgttgatg agcaatcgcc aacagggcgg
    20221 ctcacctggc ggtgtgtttg ttatgggggc gattgccgga cttatctgtt caccatgcac
    20281 caccgcaccg cttagcgcga ttctgctgta tatcgcccaa agcgggaaca tgtggctggg
    20341 cggcggcacg ctttatctct atgcgttggg catgggcctg ccgctgatgc taattaccgt
    20401 ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg gaacaagtca aaaccgcgtt
    20461 tggttttgtg atcctcgcac tgccggtctt cctgctggag cgagtgattg gtgatgtatg
    20521 gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt ggctgggcct ttatcaccag
    20581 cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata atcctgctgg cagcggcatt
    20641 ggttagcgtg cgcccacttc aggattgggc atttggtgcg acgcataccg cgcaaactca
    20701 gacgcatctc aactttacac aaatcaaaac ggtagatgag ttaaatcatg cgctcgttga
    20761 agccaaaggc aaaccggtga tgttagatct ttatgccgac tggtgcgtcg cctgtaaaga
    20821 gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa gcgttagcag acacggtctt
    20881 acttcaggcc aacatcacgg ccaacgacgc acaagatgtg gcgctgttaa agcatcttaa
    20941 tgtccttggc ctaccgacaa ttctcttttt tgacggacaa ggccaggagc atccacaagc
    21001 acgcgtcacg ggctttatgg atgctgaaac cttcagcgca catttgcgcg atcgccaacc
    21061 gtgaacgaca cttgcagcgg gaaagacgga ggaaaatagc gtgcaacgtg aagatgtact
    21121 gggagaagcc ctgaaattat tagaattaca agggattgcc aacaccacgc tggagatggt
    21181 tgctgaacgt gtggattatc cactggacga gctacgccgc ttctggccag acaaagaggc
    21241 aatcctctac gatgcgctgc gttatcttag ccaacagata gatgtctggc gtcgtcagct
    21301 gatgctggac gaaacgcaaa ccgccgaaca aaagcagctg gcacgttatc aggcgttatc
    21361 ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt atcgctgcct gtacgtttta
    21421 tcccgatcct ggccacccta ttcatcaact ggccgatcag caaaaaagcg cggcctacga
    21481 tttcacccac gaactgttaa ccacactgga agttgacgat ccggcgatgg tagcaaagca
    21541 gatggaactg gtgctggaag gctgtttaag ccgaatgctg gtgaatcgta gccaggcgga
    21601 tgtcgacact gcacatcggc tggcggaaga tatcctgcgc ttcgcccgct gccgccaggg
    21661 tggtgcattg acctgacaga aacacagaaa agaagcgatt tgccgcaatc ttaagcagtt
    21721 gaatcgcttt tactgaaatt aggttgacga gatgtgcaga ttacggttta atgcgccccg
    21781 ttgcccggat agctcagtcg gtagagcagg ggattgaaaa tccccgtgtc cttggttcga
    21841 ttccgagtcc gggcaccaaa ttcatatcaa cggacctcta cggaggttcg tttttcgttt
    21901 cagaacgcca cgatttaagc gttctgccta caaatcaatt ctaccgaact caaccagatt
    21961 ccccccacat caaccccatt gtgtgggtat aattgcgggc ataccccagt tcgacagaat
    22021 ttgtaccctc tttaacgcat tcagaaggct gaactatggc actgactgac gcaaaaatcc
    22081 gggctgcaaa gcccactgac aaggcttata aactcactga cgggactggc atgttcctgc
    22141 tggtacatcc taatggctcc cgttactggc gtctccgtta tcgtattctg ggtaaggaga
    22201 agactctggc acttggtgtg tatccagaag tttctctctc cgaagctcgt acaaaacggg
    22261 atgaggcccg aaaactgatt tcggaggggg ttgacccttg cgaacagaaa agagctaaaa
    22321 aagtagtccc tgatttacag ctctcttttg aacatattgc gcgacgctgg catgccagta
    22381 ataaacaatg ggcacaatca cacagcgata aagtactcaa aagcctcgag actcacgttt
    22441 tcccctttat cggcaaccgg gatatcacaa cactcaatac cccggatctg cttatccctg
    22501 ttcgtgctgc agaagctaaa caaatttatg aaatcgccag tcgtctgcag caaagaatat
    22561 ctgccgtaat gcgttatgcc gtacagtctg gcatcatcag atataatcct gctctggata
    22621 tggctggcgc attgactacg gtaaaacgcc agcatcgccc cgctcttgat ctttctcgcc
    22681 tgcctgaact tttgtcgcgt attggcagtt acaaagggca aactgtcacc cagcttgccg
    22741 ttatgctgaa tttactggtt tttattcgtt ccagtgaact cagatacgcc cgctggtctg
    22801 aaattgatat tgacaatgcc atgtggacta ttccagccga acgcgaaccc ctgccaggcg
    22861 taaaattctc acaccggggc tccaagatgc gaacaccaca tcttgtgcca ctcagcaaac
    22921 aggctgtagc catactgaca gaactacaga catgggctgg tgaaaatggt ctgatcttta
    22981 cgggagcaca tgacccgcgt aaaccaatca gtgaaaatgt aatgaacgca tctcacacaa
    23041 ttaaacacgg agcagtatga tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag
    23101 atttccggca tcatagtgcc caatacggga tgcataaagc tccctcagac tggagactcc
    23161 acaaatgaaa tatacaccgg ttggcgttga tatcgcaaaa catgtcattc agattcactt
    23221 catcaatgag cacacag
//
LOCUS       DAJVOY010000051        22948 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVOY010000051 DAJVOY010000000
VERSION     DAJVOY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.051"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(299..613)
                     /gene="pspE"
                     /locus_tag="OQL90_001773"
     CDS             complement(299..613)
                     /gene="pspE"
                     /locus_tag="OQL90_001773"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1368582.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(688..909)
                     /gene="pspD"
                     /locus_tag="OQL90_001774"
     CDS             complement(688..909)
                     /gene="pspD"
                     /locus_tag="OQL90_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1368583.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(918..1277)
                     /gene="pspC"
                     /locus_tag="OQL90_001775"
     CDS             complement(918..1277)
                     /gene="pspC"
                     /locus_tag="OQL90_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1368584.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1277..1501)
                     /gene="pspB"
                     /locus_tag="OQL90_001776"
     CDS             complement(1277..1501)
                     /gene="pspB"
                     /locus_tag="OQL90_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1368585.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1555..2223)
                     /gene="pspA"
                     /locus_tag="OQL90_001777"
     CDS             complement(1555..2223)
                     /gene="pspA"
                     /locus_tag="OQL90_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1368586.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2390..3367
                     /gene="pspF"
                     /locus_tag="OQL90_001778"
     CDS             2390..3367
                     /gene="pspF"
                     /locus_tag="OQL90_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1368587.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFATQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3487..4752)
                     /gene="puuE"
                     /locus_tag="OQL90_001779"
     CDS             complement(3487..4752)
                     /gene="puuE"
                     /locus_tag="OQL90_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCS1368588.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4790..6059)
                     /gene="puuB"
                     /locus_tag="OQL90_001780"
                     /pseudo
     CDS             complement(4790..6059)
                     /gene="puuB"
                     /locus_tag="OQL90_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
     gene            complement(6061..7548)
                     /gene="puuC"
                     /locus_tag="OQL90_001781"
     CDS             complement(6061..7548)
                     /gene="puuC"
                     /locus_tag="OQL90_001781"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCS1368589.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7823..8380)
                     /gene="puuR"
                     /locus_tag="OQL90_001782"
     CDS             complement(7823..8380)
                     /gene="puuR"
                     /locus_tag="OQL90_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1368590.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8407..9170)
                     /gene="puuD"
                     /locus_tag="OQL90_001783"
                     /pseudo
     CDS             complement(8407..9170)
                     /gene="puuD"
                     /locus_tag="OQL90_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9382..10800
                     /gene="puuA"
                     /locus_tag="OQL90_001784"
     CDS             9382..10800
                     /gene="puuA"
                     /locus_tag="OQL90_001784"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1368591.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10949..11114
                     /locus_tag="OQL90_001785"
                     /pseudo
     CDS             10949..11114
                     /locus_tag="OQL90_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11104..12489
                     /gene="puuP"
                     /locus_tag="OQL90_001786"
     CDS             11104..12489
                     /gene="puuP"
                     /locus_tag="OQL90_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1368592.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12623..12868
                     /locus_tag="OQL90_001787"
     CDS             12623..12868
                     /locus_tag="OQL90_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1368593.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13181..14824
                     /gene="sapA"
                     /locus_tag="OQL90_001788"
     CDS             13181..14824
                     /gene="sapA"
                     /locus_tag="OQL90_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1368594.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLKFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14821..15786
                     /gene="sapB"
                     /locus_tag="OQL90_001789"
     CDS             14821..15786
                     /gene="sapB"
                     /locus_tag="OQL90_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1368595.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15773..16663
                     /gene="sapC"
                     /locus_tag="OQL90_001790"
     CDS             15773..16663
                     /gene="sapC"
                     /locus_tag="OQL90_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1368596.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMLAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16663..17655
                     /gene="sapD"
                     /locus_tag="OQL90_001791"
     CDS             16663..17655
                     /gene="sapD"
                     /locus_tag="OQL90_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1368597.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17657..18463
                     /gene="sapF"
                     /locus_tag="OQL90_001792"
     CDS             17657..18463
                     /gene="sapF"
                     /locus_tag="OQL90_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1368598.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18531..18884
                     /locus_tag="OQL90_001793"
     CDS             18531..18884
                     /locus_tag="OQL90_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1368599.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19252..20040
                     /gene="fabI"
                     /locus_tag="OQL90_001794"
     CDS             19252..20040
                     /gene="fabI"
                     /locus_tag="OQL90_001794"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1368600.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20185..21312
                     /locus_tag="OQL90_001795"
     CDS             20185..21312
                     /locus_tag="OQL90_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCS1368601.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21380..>22948
                     /gene="rnb"
                     /locus_tag="OQL90_001796"
     CDS             21380..>22948
                     /gene="rnb"
                     /locus_tag="OQL90_001796"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1368602.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRP"
BASE COUNT         5174 a         6076 c         5988 g         5710 t
ORIGIN      
        1 aagacgcgtt acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc
       61 caggtaatca gtaattttct gtttcatgtt gcctttcagt ggtagcgatc ccatgaaacg
      121 agagactact ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga
      181 aatcattttt taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatggggat
      241 gtaaataagg tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt
      301 aacctttgac cttcggcatt gcgatgtctt tcaagccacc ggcgttctca acgtgggtat
      361 atcccatctc gctaaggatc tcttttgctt gccctgactg gcgtccggca ttgcaataca
      421 ctttcacggt gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca
      481 ggggaatatt gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat
      541 cgatccagtg ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc
      601 cttttttaaa catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga
      661 gatttaacaa tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc
      721 agcccgactt aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc
      781 aactgatttt atcgcccagc ccgctacacc cgccgggcca tagcgcagtg cggtaagaag
      841 taccagcttg cctgctaatt tgaaaccagg ctttaccttt tgcccggcct gttgccagcg
      901 agtattcata actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa
      961 gtgacataac gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact
     1021 tcatccagca attcgctgct ggagggtagc tgctcaccaa aggccatgtt gtccggcatt
     1081 ggatcaagcg caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag
     1141 aaaatcgaca gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca
     1201 atcccggcgc agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg
     1261 agattaatgc ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt
     1321 ccagcgcctg aatacgttcg cgcatccgtt ttgcttcatc agccagttgc gctaatcgct
     1381 gctgctcact ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc
     1441 agatcggtaa aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca
     1501 taagtactcc ttagacgatg agcggcggcg cgttcggacg ccgcctagat gttattattg
     1561 attgtcttgc ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc
     1621 tttcagttcg gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc
     1681 ttccgcttcc atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc
     1741 cagtttgcca ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatggcg
     1801 taacatcaat gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcaccgct
     1861 ctctttcttc atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagactctt
     1921 aatcagatcg gtcagtttct gtttttcaat taacgccgca cgtgccaaat cctctctctc
     1981 tttcagcagc gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc
     2041 aatacggcga gtcagctgtt tcttttctgc caacgcacgc gccgaagtag aacgtacttc
     2101 aaccagtgta tcttccatct cctggatcat cagacgcacc agtttctgtg gatcttccgc
     2161 tttctctaac agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc
     2221 cataatgttg tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat
     2281 ttgcgtgcca attttttatc tttttgattt ataaagatct gattgaagaa tcatcagcaa
     2341 catgccagga tgaattagcg aattacacta acaagtggcg aatttcatca tggcagaata
     2401 caaagataat ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca
     2461 tctcgcaccg ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagct
     2521 gattgccagc cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa
     2581 ctgcgcggcg ttaaatgaaa atctgctgga ttccgaactg tttggtcacg aagcgggggc
     2641 gtttaccggt gcgcaaaaac gtcatccagg gagatttgaa cgtgccgacg gcggtacgct
     2701 atttcttgat gaactcgcta cggcaccgat gatggtgcag gagaaattat tgcgcgtgat
     2761 tgagtacggt gaactggagc gcgttggcgg cagccagcca ttgcaggtga atgtgcggtt
     2821 ggtatgcgcg acgaatgccg atctcccggc gacggtcaat gaaggcactt ttcgcgctga
     2881 cctgctcgac cgactggctt ttgatgttgt acaactgcca ccactgcgcg agcgcgaaag
     2941 cgacataatg ttgatggcag aacactttgc cacccagatg tgtcgggaaa tcaagctgcc
     3001 tctgttcccg gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg
     3061 aaatattcgt gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga
     3121 ttatccgctt gatgacatca ttattgatcc ctttaaacgg cgtccgcctg aagacgctat
     3181 cgccgtttca gaaaccacct cgcttccaac actgccgctg gatttacgtg agtttcagat
     3241 gcagcaggaa aaagagttgc tgcaactcag tttgcaacaa gggaaatata accagaagcg
     3301 cgcggctgaa ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca
     3361 gatttagcga agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat
     3421 acataatcaa taagtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt
     3481 gggctattaa tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc
     3541 cggaatggtc agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag
     3601 gagcagcccc tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc
     3661 gcccgtttgc ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc
     3721 aatggccgga acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg
     3781 attcgcgcgt tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc
     3841 caccgccagc gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat
     3901 aatattcgcg ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat
     3961 cgtcattaag tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc
     4021 aaagccgctt tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg
     4081 aatggcggca accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc
     4141 gaaaataatg gccgccacct gcttcgcttc aatgtccgat ttaaacaagc gttcgatggc
     4201 gtcgagggag tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac
     4261 cgaaccaggg aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat
     4321 ggtcatatat gtacgaccgt ggaagccgcc gctaaacgca atcacgccag gtcgtccggt
     4381 atgggcgcga gcaattttca ccgcgttttc caccgcttcc gcgccggtag tgaagaatgc
     4441 ggttttagcc tgcccgctca ctggggcaag ggcgttaatt ttctccgcca gcgtgacgta
     4501 gctttcgtac ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt gctgctccac
     4561 cgccgcgacc agatcagggt ggcgatgccc ggtattcagc accgcaatgc ctgcggcgaa
     4621 atcgatgtat tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg actgggcgaa
     4681 gaagttacac atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc
     4741 attgttgctc atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca
     4801 gacgatcgcg caggctgtaa taagccgcgc ccatcgcggt aaatggcacg cgcagcgtgc
     4861 gcccgccggg gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgc tcggcgtcac
     4921 cgcgcagcag ttcagcaatc aaacgcccgg cgaggtgggt acaggtgacg ccgtggccgc
     4981 tgtagccctg catgtaatag atgttggtat cgaggcgacc gaactgcggc atccgtgaaa
     5041 gggtcagcag gaagttgccc gtccagcgat agtcgatttt gacgcccttc agctgcggga
     5101 aggttttcag cagtttcggc acaacaagac gctcaacgtc atccgggtcg cgcgcgccgt
     5161 agaccacgcc gccgccgtac agcaaacggt tgtcggcggt aagacggtag taatcaagca
     5221 gataattaca gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg
     5281 acagccgttc ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gccagttccg
     5341 gctctacttt atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct
     5401 gacctttggc agtgcgcaca acggctggcg tggtgtgctg gatctgcgtc acggcagaaa
     5461 gttcatacac gcgcccgccg ttgaggcgga tggcgtccgc ttcgccaatg gcgaggttta
     5521 gcgggtgaat atgcccaccg ctgtgatcca gcagcgcacc ggtatagcga tcgctggcta
     5581 cttcacggcg aatggcgttg gcgtccagca attccagctg tttattgccg tagcgttccc
     5641 agttctcttt ctgctcttca agtgtggcga gctgtttatc attcatcgcc acaaacaggc
     5701 tgccggggcg gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcac
     5761 caccttcgaa catcatgctg ccgagcatag gggctgtgtc catgccgtag cttttttcga
     5821 tcacgtcgat gtcgcggttg taggagttca caagctgccc gccattgcgc ccgcttgcgc
     5881 caaagccgat gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca
     5941 gcgcggagga gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact
     6001 cattcagggt gtcgaatggc acgtatttat gcgtaataac tgctggtatg ttcggtcatt
     6061 tcaggcctcc aggcttatcc agatggtttt cagttcggtg aatttttcaa gggcatgcag
     6121 ggatttgtcg cgaccgttgc cgctctgctt atagccgcca aacggcacgg tcatatcgcc
     6181 gtcgttgtag ttattgacga agacggaacc ggctttcagg cggcggctca tacggtgcgc
     6241 gcgggagagg tcgcgcatcc ataccgccgc gccaaggccg tactggctgt cgttggcaag
     6301 ctgtagcgcc tgttcttctg atgtgaaacg cgtgaccacc agcaccggac cgaaaatctc
     6361 ttcgcggctt aaggacgcat tcgggtctac atccacaaag atggtcgggc cgatggcggc
     6421 agccagcccg gcgttacggc catccagcag cagttgccct ttgccttcgc cctcacgaat
     6481 aaagctatgg accgagtcgg cgtgggcgca gtcgattaaa gtgcccatgg tggttgcggg
     6541 gtcaagcgga tggcccggct gccagttttg cgcctgctgt tttaacaggg ctaagaattc
     6601 atcggcgatg ctctcttcca gcaacaggcg tgttccggcg atgcacacct gaccctggtt
     6661 gtagaagatg cctgctgcgg tggagctcgc cgctttttgc agatccgaac aatcggagaa
     6721 aacgatgttg gcgcttttgc cgcccgcttc cagccagacg cgtttcatgt tgctgtcgcc
     6781 cgcatctttc agcagctgtt tcccggtacg ggttgaaccg gtaaaggcaa tggcgtcgat
     6841 atcgttatga cgcgacagcg cctgccccgc ttcatgacca aaacccgtca ccacgttcaa
     6901 cacaccatcc ggcaagcctg cttctttcgc cagccccgcg agacgaatcg cactgagcgg
     6961 tgatttttca gacggtttta gaatcacgct gtttcccgcc gccagcgccg ggccgagttt
     7021 ccagcaagtc agcaacagcg ggaagttcca cggcacgatg gcggcaatca cgccgaccgg
     7081 ttcacgcacg atcatcgcca gctcatggct actggtggtc gccacttcgc catacacttt
     7141 gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg ccgggaatat catcacgcag
     7201 actgtgacga atcggtttgc cggtgtcgag agtttccagc agtgccagct cttcggcgtg
     7261 ggcttccatt aaatcggcga gtttattcag taccgcttta cgtttagccg gagaagagag
     7321 tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc accgcacggt cgatatcgac
     7381 gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg actggatcaa cggtttcaaa
     7441 ggtttcattt tccgccgcag cagtatattc accgttaata aataagcggt tttcaatggc
     7501 gagacttaac gctttatcct gccagtaagc cagatgatga aaattcatta tgactcctgt
     7561 ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg cgcagagatc cccgggccgt
     7621 atgcccgttt atttctggcg acattcttta tatacacttc atccttcaag ccgcctctgc
     7681 cttggctgcg ttcgctcccc cgaatcactt acctgagtaa gctcatcggg attcgctcac
     7741 ttgccgcctt gatgcaactt gaatgatttt gtgtatagat tgccgggcgc attgcgctta
     7801 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     7861 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     7921 gacccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     7981 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8041 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     8101 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     8161 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     8221 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     8281 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     8341 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     8401 tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga taaagccctc
     8461 gaacaatata cgcgaaagag cgtactcgct actgttccat tccgggtgcc actgtacgcc
     8521 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     8581 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     8641 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     8701 atgcgacggt gcatattgct gttccaccgg cagttcggat cttcccgatg ttccagcaat
     8761 tcaggctgtt cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac cagttcttgt
     8821 aaaccccggc agatggcgaa aatggggatg cgcctttcga gtgcggcatt aattatcgcc
     8881 atgctcagaa gatcacgccc gggatcggcg tcaggctcat cgccgttttc accatatagg
     8941 tgcggctgca cattgctggg actaccagga agataaatgc catcgagttt cggcaaaagt
     9001 tgttcaagta acgacggttc cgccagcgca tgtggtagcg caattggcaa gccgcctgcg
     9061 tggatgatgg cattcaggta cttttcttgc agagtctggg tcgcatgacc cttaagcctg
     9121 ttcctgcaca ttacgacacc gataaccgga ttgttcatta tatttttcat gctcaatctc
     9181 acaaagtgga ctaaattatc gccattactg cgctttaaat gacctttatg ttcaatattt
     9241 tttcaatcta gcagtgggtt ttcatttttg caaactaaat ttaacattta gcaaacattt
     9301 agtttgcata cagattcgaa tggtggtcat tatattttac gctttgataa cgagcggaaa
     9361 acaaaccaaa ggcgaagaat catggaaacc aatatcgttg aagtagagaa ctttgttcag
     9421 cagccagaag agaggcgggg tagcgccttt acgcaggaag tgaaacgcta cctggagcgc
     9481 tacccgaata cgcaatatgt tgatgtttta cttactgatt tgaatggttg cttccgtgga
     9541 aaacgtatcc ctgtttcaag cctgaagaag ttggagaaag ggtgttattt cccggcctcg
     9601 gtgtttgcaa tggatattct gggcaacgta gtagaagaag cgggtctggg tcaggaaatg
     9661 ggcgagccgg atcgtacttg tgttcctgtt ctcggttcct taaccccttc tgccgccgat
     9721 ccagagttta tcggtcagat gctcctgacc atggtcgatg aagatggcgc tccctttgac
     9781 gttgagccgc ggaacgttct taaccgcctg tggcagcagc tacgccagcg cggattgttc
     9841 ccggttgtag cggtagagct ggagttctat ttactggatc gtcagcgcga cgctgaaggg
     9901 tatctgcaac cgccctgcgc gcccggcacc gataaccgca atacacaaag ccaggtttac
     9961 tccgttgata acctcaacca cttcgccgac gtgctcaatg atattgatga actggcgcag
    10021 ttacagttaa ttcccgccga cggtgcggtc gccgaagcct cgccgggtca gtttgaaatc
    10081 aacctttacc ataccgataa cgtgctggaa gcctgcgatg atgcgctggc gctaaaactc
    10141 ctggtgcgtc tgatggcaga aaagcataag atgcactcca cttttatggc gaagccgtat
    10201 gaagagcacg cgggcagcgg gatgcatatc catatcagta tgcaaaataa tcgtggcgag
    10261 aatgtgcttt ctgacacaga aggcgaagat tcgccgctgc tgaaaaagat gctcgccggg
    10321 atgattgacc tgatgccgtc gtcgatggcg ttgctggcac caaacgtgaa ctcgtatcgc
    10381 cgcttccagc cgggaatgta tgtgccgacg caggcgtcgt ggggccataa caaccgcacc
    10441 gtcgccctgc gtattccgtg cggcgaccgt cataatcacc gcgtggaata tcgcgtggcg
    10501 ggtgccgatg ccaacccata tctggtgatg gcagcgattt ttgccggtat tttgcatggc
    10561 cttgataacg agctgccgtt gcaggaagaa gtcgaaggca acgggctgga acaggaaggc
    10621 ttacccttcc cgattcgcca gagcgatgcc ctgggtgagt ttatcgagaa tgatcacctg
    10681 cgccgctatt taggcgaacg gttttgccat gtgtatcacg cctgtaaaaa tgatgagctg
    10741 ttgcagtttg agcgcctcat cacagaaaca gaaattgagt ggatgttgaa aaacgcatag
    10801 taccccttgc ggggttgttt catcgacctg agcggagcca ggcctgacat ttgctggtaa
    10861 tccagacatt tcccggatgt cgcgttatgg agcgcaggcg gcaacgggct tccgttaacg
    10921 attaagactg tgacgaggaa atgagatgat gccgatgatt aaatccccgc atggcgaggg
    10981 aggctgcgta tgcgcccctc ctgctactga ctggactccg tcccctttgc ttcccttgct
    11041 aaacaggttt gatttcaggt cgacacgacc gcaaacctta ttacgcaggg ggaggcagca
    11101 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    11161 aaatcactga aattgtggca ggtggtgatg atgggtctgg cctatctcac gccgatgacc
    11221 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    11281 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    11341 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    11401 tttatggtcg gctggtcatc gctgctggat tatctctttt tgccgatgat caacgtcttg
    11461 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtagta
    11521 accttcgtcg ccattttaac cgccgcgaat ctgaagagca tcaacctggt cgctaacttc
    11581 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtagtt
    11641 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    11701 gagaacgcgc acctgatccc gattattacc ggggcgacga ttgtctgttt ctccttcctc
    11761 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    11821 aaagccatct tcctgacggc ggtctacggt ggcgttatct ttatcgcggc gtcgttcttt
    11881 atgcagctgt tctttcccga tatcagccgc tttaaagatc cggatgccgc actgcctgaa
    11941 attgcgctgt acgtcggcgg caagctcttc cagtcgattt tcctctgcac cacgtttgtg
    12001 aacacgttag cctctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12061 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    12121 ccggcactca acgtcattat ggtcgggatt gtcgcgttgt cggcgctgtt cttcgattta
    12181 gttaccgcca cagcgttaat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    12241 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    12301 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaacctc
    12361 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttatgg
    12421 tatctgatcc gccgctatcg caaagtgccg ttgtatgatg gtgacagaac gccggtgagt
    12481 gaaacgtaat agtcggatgc gcggcgcatt atcctgccgg agggtaatgc accgcgccat
    12541 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    12601 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    12661 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagac gctgaacgca caccgctggc
    12721 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    12781 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    12841 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    12901 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    12961 taatgggttt ctcacgatcg taccgctctg ctcctcgctg agttgttctg tgagttatag
    13021 ttatagagga agcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13081 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    13141 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    13201 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    13261 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    13321 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    13381 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    13441 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    13501 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    13561 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    13621 cttcgacagc ctgaaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    13681 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    13741 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    13801 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    13861 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    13921 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    13981 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14041 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    14101 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    14161 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    14221 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    14281 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    14341 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    14401 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcac ggttgatgga
    14461 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    14521 cttccgtcct ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    14581 cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcggcgcg
    14641 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattgccca ttttgccgct
    14701 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    14761 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    14821 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    14881 ttcgttggct tcagcttgag ctatttcacc cctcatgcgc cgttacaagg cgcgtcactg
    14941 tggaatgcct gggtgttctg gtttaacggc ctgatccact gggattttgg tgtttccagc
    15001 attaacggtc aaccgattgc cgaacagtta aaagaggtct tcccggcgac gatggaactg
    15061 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    15121 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    15181 atcccggtgt tctggctggc gcttctgttg acgctgtttt gttcactcac gctaggttgg
    15241 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    15301 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    15361 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    15421 ctgatgcgta tcagcactat cgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    15481 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    15541 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    15601 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    15661 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    15721 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    15781 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    15841 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcggggctgg ctgtactgtg
    15901 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    15961 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16021 cggacgcgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16081 tgtggtgacg cttgccgcga cgatctgtgg cctagtactg ggcaccttcg ccggggcgac
    16141 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg caatcccttc
    16201 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgct
    16261 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    16321 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    16381 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    16441 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    16501 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    16561 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    16621 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    16681 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    16741 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    16801 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    16861 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    16921 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    16981 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17041 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    17101 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    17161 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgacc
    17221 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    17281 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    17341 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    17401 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    17461 agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg gcgctatccc actgctggaa
    17521 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    17581 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    17641 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    17701 ccgctaccgg accggctggt ttcgccgtca gaccgtagaa gcggtaaaac ccttgagctt
    17761 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    17821 gctggcaaaa atgctggcgg gaatgataga gcctaccagc ggcgagttat tgattgacga
    17881 tcatccactg cattttggcg attattcctt ccgcagtcag cgtattcgca tgatttttca
    17941 ggatccttcg acctcgttga atccccgtca acgtatctcg caaattctgg attttccact
    18001 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18061 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    18121 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    18181 tgaagcactc gcttcactgg atatgtcgat gcgttcgcag ttgattaatc tgatgctgga
    18241 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    18301 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    18361 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    18421 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    18481 ccagattatc cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    18541 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    18601 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    18661 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    18721 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    18781 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    18841 agattattct gcaggaactg aaccgccgat caccctcacc ctgaaagggc gagggggccg
    18901 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    18961 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19021 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttagtg
    19081 taaggggaaa agcgcccacc ctgccgcagc ctgctccggt cggacctagc aactatagct
    19141 actcacagcc aggttgatta taataaccat ttatctgttc gtactgttta ctaaaacgac
    19201 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    19261 ctttccggta agcgcattct ggtaaccggt gttgccagca aactatccat cgcctacggt
    19321 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    19381 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    19441 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    19501 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    19561 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    19621 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    19681 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    19741 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    19801 gttaacgcca tctctgctgg tccgatccgt actctagcgg cttccggtat caaagacttc
    19861 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt tactattgaa
    19921 gatgtgggta actccgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    19981 gtggtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga attgaaataa
    20041 tcattctgtt ggtaaatatt gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    20101 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    20161 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    20221 tatcatgaaa cgcaatccag tactgcggag tatgacgttc tgcctctggt cccggaagcc
    20281 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagaaac gctagctccc
    20341 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg aattgttccc tgaccagctt
    20401 accgtgaccc gttcacagac cttcaccgct tatgaacgct tgtctacggc cctgacggtt
    20461 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    20521 cccgggcctg attcctctag ggaaagcaat cataggttgg cacaaatcac gcaatatgcc
    20581 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    20641 gtcggtctta ctgccaggga ctgtgtaatc attaaccaaa tcattggttt tattggcttt
    20701 caggcgcgga ccattgcgac atttcaggct tatctcgggc acccggtacg ctggttaccc
    20761 ggtctggaaa tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    20821 cgaagcagct atgaggtgga aaaactacct gaagagtaca caaaaagttc aacagcagaa
    20881 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaagg
    20941 ttgttaaaca gtacacgggt taatacacag ccggataata agcttgcggc gttgttatgc
    21001 gcgcggataa atggcagtcc tgcttgtttt gccgcctgta tggattcatc aaatgaatat
    21061 aaaaaaatca gcccccttct gcgcaagggc gaaaatgaaa ttaaccaatg ggctgaccgt
    21121 cattctgttg agcacgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    21181 tttagcaccg cccagttcag ccctttactc gaacacgaac aatcatcaac gcagattatt
    21241 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    21301 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    21361 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    21421 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    21481 cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    21541 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    21601 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    21661 tgaccgtctg gccatcgttc ctgatcatcc actcttaaaa gacgccattc cttgccgcgc
    21721 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    21781 tcatccgctg aaaggcgatc gttctttcta tgctgaattg acacaataca tcacttttgg
    21841 tgacgaccac tttgtaccgt ggtgggttac acttgcgcgc cataatctgg aaaaagaagc
    21901 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgaccgc
    21961 gctggatttt gtcaccatcg acagtgccag cacagaagat atggatgacg cccttttcgc
    22021 taaggcgttg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22081 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    22141 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    22201 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcgccat
    22261 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcaaagc tggtgtatga
    22321 ccaggtttct gactggctgg aaaataccgg tgactggcag cctgaaagtg aagcaattgc
    22381 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    22441 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    22501 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    22561 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    22621 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    22681 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    22741 tgaactggac gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    22801 tgctgaaatt agcactgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    22861 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    22921 tatcaaaggc gaaactgcga cgcgccca
//
LOCUS       DAJVOY010000052        22940 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVOY010000052 DAJVOY010000000
VERSION     DAJVOY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.052"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(29..772)
                     /gene="fsr"
                     /locus_tag="OQL90_001797"
                     /pseudo
     CDS             complement(29..772)
                     /gene="fsr"
                     /locus_tag="OQL90_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
     gene            990..2642
                     /gene="ushA"
                     /locus_tag="OQL90_001798"
     CDS             990..2642
                     /gene="ushA"
                     /locus_tag="OQL90_001798"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1368603.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2679..3158)
                     /gene="ybaK"
                     /locus_tag="OQL90_001799"
     CDS             complement(2679..3158)
                     /gene="ybaK"
                     /locus_tag="OQL90_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1368604.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3362..4156)
                     /locus_tag="OQL90_001800"
     CDS             complement(3362..4156)
                     /locus_tag="OQL90_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1368605.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4294..4635
                     /locus_tag="OQL90_001801"
     CDS             4294..4635
                     /locus_tag="OQL90_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1368606.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4850..7354)
                     /gene="copA"
                     /locus_tag="OQL90_001802"
     CDS             complement(4850..7354)
                     /gene="copA"
                     /locus_tag="OQL90_001802"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1368607.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPLW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7616..8548
                     /gene="glsA"
                     /locus_tag="OQL90_001803"
     CDS             7616..8548
                     /gene="glsA"
                     /locus_tag="OQL90_001803"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1368608.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8551..9843
                     /locus_tag="OQL90_001804"
     CDS             8551..9843
                     /locus_tag="OQL90_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1368609.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9968..10375
                     /gene="cueR"
                     /locus_tag="OQL90_001805"
     CDS             9968..10375
                     /gene="cueR"
                     /locus_tag="OQL90_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1368610.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10376..10834)
                     /locus_tag="OQL90_001806"
     CDS             complement(10376..10834)
                     /locus_tag="OQL90_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1368611.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10831..11748)
                     /locus_tag="OQL90_001807"
     CDS             complement(10831..11748)
                     /locus_tag="OQL90_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1368612.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11894..12571
                     /gene="fetA"
                     /locus_tag="OQL90_001808"
     CDS             11894..12571
                     /gene="fetA"
                     /locus_tag="OQL90_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1368613.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12558..13337
                     /gene="fetB"
                     /locus_tag="OQL90_001809"
     CDS             12558..13337
                     /gene="fetB"
                     /locus_tag="OQL90_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1368614.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13400..14254)
                     /gene="cnoX"
                     /locus_tag="OQL90_001810"
     CDS             complement(13400..14254)
                     /gene="cnoX"
                     /locus_tag="OQL90_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1368615.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14315..15124)
                     /gene="ybbO"
                     /locus_tag="OQL90_001811"
     CDS             complement(14315..15124)
                     /gene="ybbO"
                     /locus_tag="OQL90_001811"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1368616.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15114..15737)
                     /gene="tesA"
                     /locus_tag="OQL90_001812"
     CDS             complement(15114..15737)
                     /gene="tesA"
                     /locus_tag="OQL90_001812"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCS1368617.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15708..16394
                     /locus_tag="OQL90_001813"
     CDS             15708..16394
                     /locus_tag="OQL90_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1368618.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16391..18802
                     /locus_tag="OQL90_001814"
                     /pseudo
     CDS             16391..18802
                     /locus_tag="OQL90_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19231..22940
                     /locus_tag="OQL90_001815"
                     /pseudo
     CDS             19231..22940
                     /locus_tag="OQL90_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5289 a         6004 c         6486 g         5161 t
ORIGIN      
        1 actatcagta agttggagtc attacctagc tgctgatgct cgccatatag aaatatttcg
       61 agaaaatgag gattaacaga atgctgactg ccagtaccac tttgttgcgc ggcagtggat
      121 tgattatcgt cgctttgggt tttcctttat tcattcggtg ctgtgccgag taccaacggc
      181 taatttgcgc caacaccacg atcgccagca gtgccgcaag cacaaaccag gcaacgttgc
      241 ctttgccata aggcgcgata atcaccgccg tcagcaaggg tcccagggaa ctgccaaagt
      301 tgccgccgac ctgaaagata gattgcgcca ggccatgccg cccgccggaa gccatacggg
      361 ctacgcgaga agattccgga tgaaagaccg atgaactggt accgaccagc gccgccgcca
      421 gcagaactgc gccaacactg cccgccagcg caagcagcac cagaccactt aaggtaaagc
      481 acatgccaat tggcaacgac cacggcatcg gatatttatc ggtccagtag ccgaccactg
      541 gttgcagtag cgaagaggcg agctggaagg tgagggttat catgccaatc tgcataaatg
      601 tcagagaaaa ttctgactga agcagaggat aaatcgccag aatcagcgat tggatcatgt
      661 cgttcagcag atgtgagagg ctgatagcac ctaaaatacc aaacgatgtt cgggccttgg
      721 tcgttgacgc agccgcgccc gccacaggct gggtttgttc actcattgcc ataggaaagt
      781 cactttttca gggttgcgat gtaaagaatg atcttatttg tgattattac ccgactaaca
      841 tacctgtaag cgtcgcctga aggaagtctc aacgccgaat acagaatttc taatctggat
      901 gcagatttat cttcaccgga cgcagacttg tctattatgt tgcgtcatac tatttttcaa
      961 cacgttgaaa tcaggtcagg gagagaagta tgaaattatt gcagcggggc gtggcgttag
     1021 cgctgttaac cacatttaca ctggcgagtg aaactgctct ggcgtatgag caggataaaa
     1081 cctacaaaat tacagttctg cataccaatg atcatcatgg gcatttttgg cgcaatgaat
     1141 atggcgaata tggtctggcg gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg
     1201 cggctgaagg cggtagcgtg ctgctacttt ccggtggcga cattaacact ggcgtgcccg
     1261 agtctgactt acaggatgcc gaacctgatt ttcgcggtat gaatctggtg ggctatgacg
     1321 cgatggcgat cggtaatcat gaatttgata atccgctcac cgtattacgc cagcaggaaa
     1381 agtgggccaa gttcccgttg ctttccgcga atatctacca gaaaagtacc ggcgagcgcc
     1441 tgtttaaacc atgggcgctg tttaagcgtc aggatctgaa aattgccgtc attgggctga
     1501 caaccgatga cacagcaaaa attggtaacc cggaatactt cactgatgtc gaatttcgta
     1561 agcccgccga tgaagcgaag ctggtgattc aggagctgca acagacagaa aagccagaca
     1621 ttattatcgc ggcgacccat atggggcatt acgataatgg tgagcacggc tctaacgcac
     1681 cgggtgatgt ggagatggca cgcgcgctgc ctgccggatc gctggcgatg atcgtcggtg
     1741 gtcactcgca agctccggtc tgcatggcgg cagaaaacaa aaaacaggtc gattacgtgc
     1801 cgggtacgcc atgcaaacca gatcaacaaa acggcatctg gattgtgcag gcgcatgagt
     1861 ggggcaaata cgtgggacgg gctgattttg agtttcgtaa tggcgaaatg aaaatggtta
     1921 actaccagct gattccggtg aacctgaaga agaaagtgac ctgggaagac gggaaaagcg
     1981 agcgcgtgct ttacactcct gaaatcgctg aaaaccagca aatgatctcg ctgttatcgc
     2041 cgttccagaa caaaggcaaa gcgcagttgg aagtgaaaat aggcgaaacc aatggtcgtc
     2101 tggaaggcga tcgtgacaaa gtgcgttttg tacagaccaa tatggggcgg ttgattctgg
     2161 cagcccaaat ggatcgcact ggtgccgact ttgcggtgat gagtggagga ggaattcgtg
     2221 attctatcga agcaggcgat atcagctata aaaacgtact gaaagtgcag ccattcggca
     2281 atgtggtggt gtatgccgac atgaccggta aagaggtgat tgattacctg accgccgtcg
     2341 cacaaatgaa gccagattca ggtgcctacc cgcaatttgc caacgttagc tttgtgacga
     2401 aagacggcaa actcaacgac ctgaaaatca aaggcgaacc ggtcgatccg gcgaaaactt
     2461 accgtatggc gacattaaac ttcaatgcca ccggcggtga tggctatccg cgccttgata
     2521 acaaaccggg ctatgtgaat accggcttta ttgatgccga agtgctgaaa gcgtatatcc
     2581 agaaaagctc gccgctggat gtgagtgttt atgaaccgaa aggtgaggtg agctggcagt
     2641 aatccgaaag tgccggatat ttgcatccgg cacaatgctt aatcgcggcg ggcgatatca
     2701 gcaaatttgg catcgaggat cttcgccaga tcgcctgccg ccagttcgat atccagtccg
     2761 cgcttgccgc cagaaacata aatagtggca aattcttgtg cgggggcgtc gataatcgtc
     2821 ggcagacgtt ttttctgccc cagtgggcta atccccccaa ccaggtatcc cgtcgaacgc
     2881 tgcgcggcca tcggatcggc catctcaact ttcttggcac ccagcgcttt tgctactttt
     2941 ttaagatcca gttgacctgc gaccggcgta acggccactg ccagatgttt catatcaccg
     3001 ttcactgcca ccagtagcgt tttgtagact tgatccggat tcaaacctaa ttttttgacg
     3061 acttcatcgc caaaattggt ttcagccgga tcgtgctcgt aggtatggat ttgaaacgaa
     3121 atcttgtttt tttcgagtaa tttaactgcg ggtgtcatgg taattttcct gccttaaaca
     3181 aaaaaacgaa gcaagcatac gcctgatggc ggtccaaaaa aaagagtcat cttgcctaag
     3241 agtattggca ggatggtgag attgagcgac aatcgagtta caccgtcgct taaagtgacg
     3301 gcataataat aaaaaaatga aattcctctt tgacgggcca atagcgatat tggccatttt
     3361 tttagcgcaa catttgcggc aaattccctt ctccatacag gtgtagtgca ccgaccgcga
     3421 ccacatatcg ccccggcggc atggcgcgta atttatcccg ccaggcgaga tttcgctgat
     3481 gcatcagcac atcgtacagc gactgactga acgtattggg cagcgttata tcattatttt
     3541 gcggaggtgc attcagccac cagctcatca tttgttgcag caaccgtgcg ttggtatgcc
     3601 agtgggtcag cgtatcgtcc agcagcgcca gtcctttgtc agggagctgg agcaacatgg
     3661 taatctggtt ttcagcccct tccagttcaa tcacgggttt atgttgttgc ttcgccgcct
     3721 gcaataactg gtaatcgata ccgtattctg cccgcagccc cagtttttgt gcctgcgtcg
     3781 cctgaagaac catcgcgatt tgccacagcg gttgagtaga aaagagcgac ggagaaatgc
     3841 ccatctcctg gctaatgtgc tgcagatttt ggagttgctc ctcgttaatg cgctcttcca
     3901 gcgcctcgca agtaggcaaa ttagcaaaag gcgtatcgct ggtggaaaca tccgcctcga
     3961 cgatcagcgc atcggcgttt ttgagctttt tgagcaaacg ggtgggcagg ggagtcatat
     4021 cgtggctacc catatgaata ctgccaatca gatgaaaatg gcgattgccg ggaagggtga
     4081 tatcgatggc aggccaggtg taatgattac cgcgcagcgc ggcccaaagc gtttttaccc
     4141 ggtacaacag atccatacga cctccctttg tgaaatatca tgctagcgcg cggtgaggga
     4201 tggcgcaaat ggagttgggg acttgtttaa tgtgtttgta tgattcagta tgttcttgca
     4261 tcgctattca cagggaagca acagttaaaa accatgaaac aggcaacaag aaaaccgacg
     4321 acacccggag atattcttct ctatgaatat ctggaaccgc tcgatttgaa aatcaatgag
     4381 ttagcagagt tgctgcatgt tcatcgtaat agcgtcagta cactgatcaa taataatcgt
     4441 aaactcacca ctgagatggc attccgtctg gcgaaagttt ttgataccac agtcgatttt
     4501 tggctaaacc tccaggcggc agttgatctt tgggaagttg aaaacaacat gcgcacccag
     4561 gaagaattgg gacggattga aacagtggct gaatatttgg cacgccgtga agagcgtgca
     4621 aaaaaggtcg cgtaaggcca aaacatcgct tcatctcgta ggccggataa ggcgttcacg
     4681 ccgcatccgg catccgagca ccatagcctg atgcgacgct tacccgtctt atcaggccta
     4741 caaacctgtg cctgaaccgt aggccggata aggcgtttac gccgcatccg gcagtcatgc
     4801 gtcgatgcca aatgcgccgc cctaaagcag cgcatccgca atgacgtact tattccttcg
     4861 gtttaaaccg cagcaaccgg ttggcgttac tcactacggt aatcgacgag agcgccattg
     4921 ccgctccggc aactaccggg ttaagcagtg ttccagtaaa cggccacaaa ataccagcgg
     4981 cgaccggaat accaatgctg ttgtagataa acgcaccgag caggttctgc ttcatattgc
     5041 gcagcgttgc gcgggaaatc gcgagcgcat ctgcaacacc catcaggcta tggcgcatca
     5101 gggtaatcgc cgcggtttca atggcaacat cactgccgcc acccatcgca ataccgacat
     5161 ccgcctgagc cagcgctggc gcgtcgttaa tgccgtcgcc caccattgcc acctgacgtc
     5221 cttcactttg cagacgtttg atcgcttcgg ctttaccgtc tggcagcacc ccggcgatta
     5281 cctcatcaat ccctgcttct ttggcgatcg cattggcggt ggttgggtta tccccggtca
     5341 acatcaccag acgatatccc gctttatgca ggcgttgcag cgccgccacg ctatcactac
     5401 gcaacggatc gcgtacagcc agcagggcta ctactttccc gtcaatcgcc agcagcacag
     5461 gcgttgctcc ttgcgatgcc tgagctgaaa tatcggcttc aatcgcttta gtatcgactt
     5521 gctggtcatt taacagcgcc ggattaccca gcaataacgc atgaccttca gcttcaccgc
     5581 ttacgcccag cccgcgcaat gtgcggaaac cgttgacctg cggcagctgc atatcgcctg
     5641 ctttatcgag gatcgctcgt gccagcggat ggctggaacc ttgctccagc gccgccgcca
     5701 gacgcaatgc ttgcgcttca tcaacatcag caaatgtttt tactgcgaca acctgcggct
     5761 tcccttcggt cagcgtcccg gttttatcga acacgacggt atcgagtgta ctggcgcgtt
     5821 gcagcgcgtc ggcgtcccgc accagcacgc caaactcagc cgcccgcccg acgccggaaa
     5881 taatcgacat tggcgtcgcc agccccagcg cacacggaca ggcaataatc agcaccgtgg
     5941 tggctatcac cagggtatag acaatctgcg gtgccggacc aaagaaatac cagattgctg
     6001 cactgatcag cgcaatcacc actactaccg gcacaaatac ggctgagatt ttatccgcca
     6061 gctgaccgat ttctggcttg ctgctctggg cctggcgcac catgcgaata atccttgaca
     6121 gcgtggtatg gctgccaacc gcactggcgc gaaatagcac gctaccgtcc tgtaccactg
     6181 tcccggcatg gacgctttcg ccttcgcctt tttgctgcgg gattggttcg cccgtcagca
     6241 tcgcttcatc cagccatgct tcgccctggg taatctcgcc atctaccggc acgcgatcgc
     6301 cggtcgtcag gcgcagtaac atccctggct gaacttccgc cagcggcacg cttttttcac
     6361 cctctgcggt gaccactcgc gccgtcggtg gggttaaatc gagtaatttc tccagcgcct
     6421 tagaagaacg ctgacgcgcg cgcgcttcca gcatatggcc gagattaatc agaccgataa
     6481 tcatcgcact ggcttcatag taaagatgcc gcgcttccat cgggaaccac agcggccaca
     6541 ggttgacgct catcgaatag agccacgcca cacccgtacc cagcgccacc agcgtatcca
     6601 tcgtcgccgc accgttcagc aggcttttcc acgcgctgcg gtaaaaatgc ccgccggcga
     6661 aaaccattac cgccagggtt atcaggccga taactaacca caggctgcgg ttgtcagcgg
     6721 tgaccatcat gttatcgccg atcatccccc agaccattac cggaataccc accgccagtg
     6781 cgacaatcgc ctgccagcgg aagcgcttca tcgtagcgac ggcggtttcc tgctggcgct
     6841 cgcggcgttt cgcgtcatct tcaatcgctt ccgcgccgta gcccgctttt tccaccgcct
     6901 gcactaaatc ttgtggggag gcactgccca tcaccagcgc agtacgctcc gccaggttta
     6961 cccgtgcctg agtgacgccc ggtacgcttt gcagcgcatt ttgtacgcgg gtgacacagc
     7021 tggcgcagct catgccgctc agcagcaact gctggctgtc atcgtcatcg gcggtcgctg
     7081 ccggaagcgc ctcagaaacc gctgtcagtg cttccgacgg gattgatgac tccgccagcg
     7141 gtttagcctt tgggtggctt acagatgcgt cataacccgc ttgtttgatg gtttcgatca
     7201 gctgttctgc actggcagtc ccggtaacgt gcgcttcagt gatagacaca tccgcttgtt
     7261 ccacatccgg gcgctgttcg agactttctt tcacgcgttt aacgcagtga ccgcaggaca
     7321 ggccgtccag ggtcaggtcg atagtttgtg acataaaaca ctcctttaag acagttttga
     7381 ctggctgtga taaaggttaa accttccagc aaggggaagg tcaagaaatt aataaaccag
     7441 gcgggtaaaa gtccgtaaag attaaaaaat cggctcgatt tgcatcagga ttagacattt
     7501 atttctttgt tttcctgtag ttaagttgct ggtgctaagt taaatcagga tgcctgaaaa
     7561 tcggcaccgg ggtgaggaat tacctcccgc atctataaaa aggagttaac aaaagatgtt
     7621 agatgcaaac aaattacagc aggcagtgga tcaggcttac acccaatttc actcacttaa
     7681 cggcggacaa aatgccgatt acattccctt tctggcgaat gtaccaggtc aactggcggt
     7741 agtggctatc gtgacctgcg atggcaacgt ctatagtgcg ggtgacagtg attaccgctt
     7801 tgcactggaa tccatctcga aagtctgtac gttagccctt gcgttagaag atgtcggccc
     7861 gcaggcggta caggacaaaa ttggcgctga cccgaccgga ttgcccttta actcagttat
     7921 cgccttagag ttgcatggcg gcaaaccgct ttcgccactg gtaaatgctg gcgctattgc
     7981 caccaccagc ctgattaacg ctgaaaatat tgaacaacgc tggcagcgaa ttttacatat
     8041 ccaacagcaa ctggctggtg agcaggtagc gctctctgac gaagtcaacc agtcggaaca
     8101 aacaaccaac ttccataacc gggccatagc ctggctgctg tactccgccg gatatctcta
     8161 ttgtgatgca atggaagcct gtgacgtgta cacccgtcag tgctccacgc tcatcaatac
     8221 tgttgaactg gcaacgcttg gcgcgacgct ggcggcgggt ggtctgaatc cgctgacgca
     8281 taaacgcgtt cttcaggccg acaacgtgcc gtacattctg gccgaaatga tgatggaagg
     8341 gctgtatggt cgctccggtg actgggcgta tcgcgttggt ttaccgggca aaagcggtgt
     8401 aggtggcggt attctggcgg tcgtccctgg cgtgatggga attgccgcgt tctcaccacc
     8461 gctggacgaa gaaggtaaca gtgttcgcgg tcaaaaaatg gtggcatcgg tcgctaagca
     8521 actcggctat aacgtgttta agggctgatc atgatgaaca cggaaggtaa taacggtaac
     8581 aaacccctcg gtctatggaa tgtcgtttcc atcggtattg gggcaatggt gggggcgggg
     8641 atcttcgcgc tgctggggca ggctgcgttg ctaatggaag cctcgacctg ggtcgccttt
     8701 gcttttggcg gtattgtggc gatgttttcc ggttatgcct atgcgcgtct gggggcgagc
     8761 tatcccagca atggcggcat tatcgacttc tttcgtcgcg gattaggcaa cggcgtcttt
     8821 tcgctggcgc tctcgttact gtacctgttg acgctggcgg tgagcatcgc catggtcgcc
     8881 catgcttttg gcgcttatgc cgtgcagttt ttgcatgaag gcagccagga ggagcacctt
     8941 attttgctct acgcgttggg gagcattgcg gtgatgacgc ttttcaactc cttaagcaac
     9001 catgcggtag ggcggctgga agtgatcctc gtcggcatta aaatgatgat cctgttattg
     9061 ctgattattg ccggtgtctg gtcgctgcaa ccagcgcata tttccgtctc tgcgcccccc
     9121 agctccggtg cgttcttctc ctgtattggg ataactttcc ttgcctatgc gggctttggc
     9181 atgatggcga acgcggcgga taaagtgaaa gatccgcagg tcattatgcc acgggcgttt
     9241 ctggtggcga ttggcgttac cacgttgctt tatatctcgc tggcactggt tttgcttagc
     9301 gatgtatcgg cattagagtt agaaaaatat gccgataccg ccgtagcgca ggctgcttct
     9361 ccgctgctcg gacatgtggg ttatgtgatc gtcgtcatcg gcgctttact ggcgacggct
     9421 tcagccatta acgcgaacct gttcgccgtg tttaacatca tggacaacat gggcagcgaa
     9481 cgcgaactgc cgaagctaat gaataaatcc ctgtggcggc agagtacctg gggcaacatc
     9541 attgtcgtgg tgttgattat gctgatgacg gcggcactga atttaggctc actcgccagc
     9601 gttgccagcg ccaccttttt gatttgctac ctggcggtgt ttgtggtggc gatccgcctg
     9661 cgtcatgata ttcacgcctc gttgccgatt cttatcgttg gtacgttggt gatgttgttg
     9721 gtgatcgttg gctttatcta cagtctgtgg tcccagggta gccgtgcgtt gatatggatt
     9781 attggctcac tcttactcag ccttattgtg gcaatggtca tgaagcgcaa taaaaccgta
     9841 taacatctct ctgtgcgcag tacttcctgt attattgtgg tggcggtcga tattcgcact
     9901 ggcaaaaaaa cgtgcttgaa tatctgttga aaccctttaa caaagcacag gaggcgttgc
     9961 gcgaacgatg aacatcagcg atgtagcaaa aattaccggc ctgactagca aagccattcg
    10021 cttctatgaa gagaaggggc tggtgacgcc gccgatgcgc agcgaaaacg gttatcgcac
    10081 ctacacgcag cagcatctca acgaactgac cttactgcgc caggcacggc aggtgggctt
    10141 taacctggaa gagagcggcg agctggtgaa tctgtttaac gacccgcagc ggcacagcgc
    10201 cgacgtcaaa cggcgcacgc tggagaaggt ggcggagatc gaacgacaca ttgaggagct
    10261 gcaatccatg cgcgaccagc tgctggcact ggcgaatgcc tgccctggcg atgacagcgc
    10321 cgactgcccg attatcgaaa atctctccgg ctgctgtcat catcgggcag ggtgattaag
    10381 acgagacggc tcggatatgt agggttatcc cttctatcgc aatgacttca acatgcgtac
    10441 ctgcaccgag atcctcgctg gcgctgacag gccatgaact gtcaccgacg cgcatatgac
    10501 cgcgcccgtt gaccagcgga gattccagca caaaacgtcg gccaatcagc tgctgcccgc
    10561 gctggtttaa atgactatcg ctgtgctttt gttcgcgcac ccgccgcgac aaccatttcc
    10621 accacagcca ggcggcgagc agcgtcagga tggcaaacat taccccttgc cactcccaac
    10681 ccagcggcac cagccagacc accaggccgg taatcaccgc tgccacgccg ctccacaaca
    10741 aataaccatt tccgcccagc atctcggctg ccagcagcaa accgccgaga ctgagccaga
    10801 aaatatgtgg atgaacgacc attaactcca tcatggctga gtccgtttgt tggcgctgtc
    10861 tttcaccagt tcggcaatcc cggcaatcga ccccatcagg ctgctggcct ctaatggcat
    10921 catcactact ttgctgttac tggaggaacc gatctgctgt aacgcttcgg tgtatttctg
    10981 cgctacgaag tagttcaccg cctgaatatc accggaggcg atagcttcag acaccatttt
    11041 ggtggcgcgg gcttctgctt cggcggaacg ttcacgcgct tcagcctgta aaaacgccga
    11101 ctgacgttcg ccttccgctt tcaggatttg cgactgtttt tcaccttcgg ctttgaggat
    11161 ttccgcctga cggatccctt ccgcttcaag aatgtaagcg cgtttggtac gttccgcttt
    11221 catctgcgcg ttcattgaag agataagctc tgccggtggg cgcacgtcgc gaatttcaat
    11281 acgggtgact ttaatccccc acgggttggt ggcttcatcg acaatacgca gcaggcgtga
    11341 gttgatgctg tcgcgctgag agagcatttc gtcaagttcc attgaaccca acacggtacg
    11401 gatgttagtc atggtcaggt tgatgatcgc cagctccaga ttgctgactt cataggcagc
    11461 gcgtggcgcg tcaatcacct gaataaaaca gactgcgtcg atggtaacgt tggcgttatc
    11521 tttcgagata acttcctggg aagggatatc gagcacttgc tccatcatat tgatcttgcg
    11581 accaatgcga tccataaacg gcaccaccag actgagcccc ggctgtaacg ttttggtata
    11641 gcgaccaaaa cgttctaccg tccattgata gccctgcggc acgattttga cgcccgcgcc
    11701 gacaatgacc agcgcgacaa aaatgagaat cgggataaag ataagcatcg gaaaaacctc
    11761 ctgttgtacc gtccataatc agcaaaattg ctgcttgatt aaacaaatta tacctgatta
    11821 ctgaaagaga gttccccctt attcctgcga aggataaact gtttttagta aaaatcagaa
    11881 aaagggaaca gcgatgcagg aaaatagtcc tttgcttcag ctacaaaacg taggatatct
    11941 ggcgggtgat gcgaagattc ttaataacat caatttttcg ctgcgtgctg gcgaatttaa
    12001 gttaattacc ggtccttctg gttgtggcaa aagtacgctg ctaaaaatag ttgcttcatt
    12061 gatcagccca accagcggaa cgttactgtt tgaaggtgag gatgtcagca cactaaagcc
    12121 agaaatctac cgccaacaag tctcttactg cgcccagaca ccgacgctgt ttggcgatac
    12181 ggtatacgat aatctgatct ttccttggca gatccgtaac cggcagcctg acccagccat
    12241 ttttctcgat tttctcgaac gcttcgcctt gccggacagc attttgacga aaaatatcgc
    12301 cgagctatct ggtggtgaaa aacaacgcat ctcactgatt cgtaacctgc aatttatgcc
    12361 gaaggtttta ttgctggatg aaatcaccag tgcgctggat gaaagtaata aacataacgt
    12421 taatgagatg atccatcgtt atgtgcgcga gcaaaatatt gccgtgctgt gggtgacaca
    12481 cgataaagac gaaattaatc atgcggataa agtgattaca ctgcaaccgc atgccggaga
    12541 aatgcaggaa gcacgctatg aactcgcata atattactaa cgaatcattg gcactggcat
    12601 taatgctggt ggtggtggca atcttaatta gccataaaga aaaactggcg ctggagaaag
    12661 atattttctg gagcgtcggg cgcgcgataa ttcagctgat tattgtcggc tatgtgctga
    12721 agtatatttt cagcgtggat gatgccagcc tgacattatt gatggtgtta tttatctgct
    12781 ttaatgcggc gtggaacgcg caaaaacgca gtaaatatat tgctaaagct tttatctcat
    12841 cgtttattgc cattacggtt ggggcgggaa ttaccctggc ggtgctgatt ctctcagggt
    12901 cgattgaatt tatcccgatg caggtgatcc ctatcgccgg gatgattgcc ggtaacgcca
    12961 tggtagcggt ggggttgtgt tacaacaatt tagggcaacg ggtcattagc gaacagcaac
    13021 agattcagga gaaactgagt cttggcgcga cgccgaagca ggcttcagcg atattgattc
    13081 gcgacagtat tcgcgcggct ttaattccga cggtcgattc agcaaaaacg gttggcttag
    13141 tgagtttacc aggaatgatg tccgggctga tatttgccgg gattgatccg gtgaaggcga
    13201 ttaaatatca gattatggtg acctttatgc tgctctcaac cgccagcttg tcgaccatta
    13261 ttgcctgcta tttaacctat cgtaagtttt ataattcgcg ccaccagttg gtggtgacgc
    13321 aattgaagaa gaaatgatgc gatgccggat gcgcagcatc gcatccggcg ttgtggttca
    13381 tgtgccgaac aacggaatat caatacaaca atgcatacag ctggcggcga tacttcgacg
    13441 ccagtgcatc acccgtaccc agcgcagcga ggatctcctg gaacgtttta cgcgtctgac
    13501 cgtctgcggc ggtgagatct ttacgcagat gcccgaacag caactccagc gcctcttcat
    13561 tgcgcccaac ctgatgcagt tgcagcgcca gttgcgtcgc cagtgcggca tcttctggat
    13621 tctccgccac ctgctgttgc aactgttgaa tttccggcgt atcagccgcc tgcttcagca
    13681 gttcgatttg cgccaccagc ccctggtagc gggtgtcctg atcctgcaac ggaatggttt
    13741 tcagcaccgc ttccgcatct tcagaacggt tcagcgcaat cagcgtttct gccagcagca
    13801 ggccgatctc cccgttctga ttcgacaact gccaggcgtc tttcagcaat ggcagggcat
    13861 cggtgtaatt gccttcctgc atcagttgca tcgcctgctg cgctttcagc tcttcttcgc
    13921 gcggcagcac tttatccagc agggcgcgga tcgcctcttc cggttgcggc ccctggaagc
    13981 catctaccgg ttgcccgttc tggaacagat acacggtcgg aatcgcgcgc agaccaaact
    14041 gggcggcaat catctgctcc gcgtcgcagt ccagcttcgc cagaataaat tgcccgttgt
    14101 actgcgccgc gaggctttcc aggatcgggg ttaactgcaa acagtgctgg ctacgttcag
    14161 accaaaaata gaacagcacc ggagtggtca tcgactgttc aagaacctgt tgcaggttag
    14221 attcgttaat gttgacaata ttttctacgg acatggagtc gctctctgtt gtcgatattt
    14281 tctttgacat gggggcttaa gcgcgcgctt caactcaccc ctgcaatatt ttgtccatca
    14341 cgcgcccagg cagcaggcgc ttgagcacca ttactgccca ggtcaccagc gttaccggat
    14401 agcgcatctt cggcttctcg ctaataaaag catggcgcac tttgtcgacc accgcttccg
    14461 gtcccaacgt aaagcgggcg gcgatgccgg gattttcgac tggtttatca ctttgcgtct
    14521 ggttgacgtt gtcggtgaag cgagtacgaa tgggaccggg ttcgatcagg ctgactttaa
    14581 ttccgctgtg gcgtagctcc atgcgcagcg cgtctgacca cgcctccagc gcatatttac
    14641 tggccgcgta agcgccacga cccggcgtgg agattaatcc catcaccgat gatgtcatca
    14701 caatacgccc ttcaccgtgc ggtaacatcg cgggtaacag gcgcatggtg agctggtgtg
    14761 cgccgaaaaa gttggcggaa aactgctgtt ccatctgcgc acggctgatg gtggaaaggg
    14821 ggccatacat gccgaatccg gcattgttaa agatcccata cagacaatta tcggtcaggg
    14881 cgatcacctc gtctgctgcg cgatcaacac tttctggtga atccagatcg atcaacacgc
    14941 cggtaaatcc catgttgttc atacgctcaa catcatccgg ttttcggcaa cctgccagca
    15001 catgaaaacc ctggcgtttt aattcgagcg cgctttccag gccaattcca ctggaacatc
    15061 cggtaattaa gaccgatttt tgcataactt tacctgtcag gatctccgtt gctttatgag
    15121 tcatgattta ctaaaggctg caactgcttc gccatccagt cggcaataaa cggctgggcg
    15181 tcgcggttgg gatgaatacc gtcatcctgc atccattgtg gcttgaggta gacctcttcc
    15241 ataaaaaagg gcagcagcgg aacatcaaac tctttggcga gtttggggta aatggcgcta
    15301 aaggcttcat tataacggcg accatagttt gcaggcagac gtatttgcat taacaatggt
    15361 tcagcgttgg cggctttgac atcctgcaaa atctggcgca gcgtttgctc ggtttgctgt
    15421 ggctgaaaac cacgcaaacc gtcattgccg cccagttcaa ccagcaccca gcgcggctga
    15481 tgctgtttca gcagagccgg aaggcgcgcc agtccttgtt gcgaggtgtc gccgctgatg
    15541 ctggcattaa ctaccgacgt tttactctgc cacttatcat tcaacaaggc aggccaggcc
    15601 gcgctggcag acattcgata cccggcgctc aggctatcac ccagaatcaa taacgtgtcc
    15661 gctgcggcgg cacggaaggt taacaggacc aggaacagga agggcaaatg ccagcggaaa
    15721 acattgttga agttcatcat cttaagaagt ccgtcggtca gggggagcat gaactctcca
    15781 tcctcaccgg agttgagctg gttgtcaaac gtggcgagac catcgcactg gtgggcgagt
    15841 cgggatcggg taagtcaacc ttgctggcga tcctcgccgg gcttgatgac ggcagcagtg
    15901 gcgaagtgag tctggtggga caaccgctac ataatatgga cgaagaagcg cgggcaaagt
    15961 tgcgcgcgaa gcacgtcggc tttgtttttc agtcatttat gttaattcct acccttaacg
    16021 cgctggaaaa cgtcgagctt ccggctctgc tgcgcggtga gagtagcgcg gaaagtcgta
    16081 acggggcgaa agcgttgctc gaacagttag ggctgggtaa acgtctggat catcttccgg
    16141 cacagctttc cggcggtgaa cagcaacgag tggcgctggc acgagccttt aatggtcgac
    16201 ctgatgtgct gtttgccgac gaacccaccg gcaaccttga ccgccagacg ggcgataaaa
    16261 ttgccgacct gctgttttcc ctcaaccgtg aacatggcac cacgttgatt atggtgaccc
    16321 acgacctgca actggcggca cgctgcgacc gctgcttacg gctggtgaac gggcagttgc
    16381 aggaggaagc atgattgcac gttggttctg gcgcgaatgg cgttcgccgt cgctattaat
    16441 tgtctggctg gcgctaagcc tggcggtggc ctgcgtgctg gcgctgggca atatcagcga
    16501 tcgcatggag aagggcttaa gccagcaaag ccgtgagttt atggcgggcg atcgggcgtt
    16561 gcgcagttca cgcgaagtgc cgcaagcgtg gctggaggaa gcgcaaaagc gcggcctgaa
    16621 agtcggcaag cagctgactt tcgccacaat gacctttgca ggcgacacac cgcagctggc
    16681 gaacgtcaaa gcggtggatg atatctaccc gatgtatggc gatctgcaaa ctaatccccc
    16741 tggcctgaaa ccgcaggcgg gcagcgtatt gctggcccca cgcctgatgg cactgcttaa
    16801 cctgaaaacg ggcgacacca ttgacgtggg cgatgccacc ttgcggattg ccggagaagt
    16861 gattcaggaa ccggattccg gttttaaccc cttccagatg gctccgcgtc tgatgatgaa
    16921 tctggcggat gtcgataaaa ccggagccgt gcagccgggg agtcgggtca cctggcgtta
    16981 taaattcggc ggcaacgaga accagctcga cggctatgag aaatggttgt tacctcagct
    17041 taaacccgaa caacgctggt acggtctgga acaggacgaa ggcgcgctgg ggcgatcgat
    17101 ggaacgctcg caacagttcc tgctgctttc ggcgcttctg accttgctgc tggcagtggc
    17161 agcggtggcg gtagcgatga atcattactg tcgcagtcgc tacgatctgg tggcgatcct
    17221 caaaacgctg ggggcagggc gaacgcaact gcgtaagcta atcgtcggtc agtggttgat
    17281 ggtgctgacg ctttcagccg ttaccggtgg ggccataggc ctgttgttcg aaaacgtgtt
    17341 gatggtgctg ctcaagcccg ttctgcctgc tgcactaccg ccagccagcc tctggccgtg
    17401 gctgtgggcg cttggcacca tgacggtcat ctcgctgctg gtggggctac gaccataccg
    17461 cttgttgctg gcaacgcagc ctttacgcgt attacgtaat gatgtggtag cgaacgtctg
    17521 gccgctgaag ttttatctgc cgattgtcag tatggtggtt gtgctgctgc tcgccggatt
    17581 aatgggtggc agcatgctgc tttgggcggt gctggcgggc gcagtagtac tggctttgct
    17641 gtgcggtgtg ctgggctgga tgcagctgaa tgtactttgc cgcatgacgc tgaaatcgct
    17701 acctctgcgc ctggcggtta gccgcctgtt acgtcagccg tggtcaacgt taagccagct
    17761 ttcggcattt tcgctctcct ttatgctgct ggcgctgttg ctggtattgc gttgcgatct
    17821 gctcgaccgc tggcagcagc taccgccaga aagcccgaac tactttttaa ttaacatcgc
    17881 cacagaacag gtagcaccgc taaaagcgtt cctcgcggaa catcatatag tcccggaatc
    17941 attttatccg gtagtgcggg cgcggctgac ggcgattaac gataagccga cagaaggtaa
    18001 tgaagatgag gcgcttaatc gcgaactcaa tcttacctgg caaaatacgc ggcccgatca
    18061 taatccgatt gtcgccggta actggccgcc aaaagccgat gaagtgtcga tggaagaggg
    18121 gctggcgaaa cgcttaaacg ttgccctcgg tggtaccgtg acttttatgg gcgataccca
    18181 ggagttccgc gctaaagtga ccagcctgcg caaagtggac tgggaaagtc tgcggcctaa
    18241 tttctatttt attttcccgg aaggggcatt agacgggcaa ccgcagagct ggcttaccag
    18301 tttccgctgg gagaatggca acggcatgtt gacgcaactc aaccgccagt tcccgaccat
    18361 tagcctgtta gatattggcg cgattttaaa acaggtcggt taggtgctgg agcaggtaag
    18421 tcgggcgctg gaggtgatgg tggtgctggt caccgcctgc ggtatgttgc tgttgctggc
    18481 acaggtgcag gtgggaatgc gccagcgtca tcaggagctg gtggtatggc gcacactcgg
    18541 tgcggggaaa aaactgctgc gcacgacatt gtggtgtgag ttcgccatgc ttgggtttgt
    18601 ttccggcctg gtggccgcaa ttggtgcgga aacggcactg gcagtgttgc aggcgaaagt
    18661 gtttgatttc ccgtgggagc cagactggcg attgtggatt gttctgccgt gcagcggtgc
    18721 gctgctgctg tcgcttttcg gcggctggct gggtgcgcga ctggttaagg gtaaggcgct
    18781 gttcaggcag tttgcggggt gatgagagta aataaggggc gttctggttg aatggaacgc
    18841 cttgtttaaa ttaattctac aatatgtttc attgatgatg ttattgaatt ggtgatttcc
    18901 tatctttcta ttgctgatat taatctgaat tggtggagtt taataatttt aaatatagcc
    18961 ctacaaaaat aactcagact aataaaatca taaatcatat gcgttgaatg gatattatcc
    19021 atatagtgaa tttgttgatg atgaattcat ctgtgctaaa aattttagtt taataaaata
    19081 ttgaaagtga cctgtaataa cagttattgt tgattgagaa caaataagtt tatgtgaaaa
    19141 atatataaat acattagctg gtcatgtgtg tcattttatt tttttgttgc taacacaggg
    19201 atatgaacaa taactaaaag ggcactttat atgagcggaa aaccagcggc gcgtcaggga
    19261 gatatgactc agtatggcgg tcccattgtc cagggttcgg caggtgtaag aattggcgcg
    19321 ccaactggcg tggcgtgctc ggtgtgtccg ggcgggatga cctcgggcaa cccggtgaac
    19381 ccgctgctcg gtgcaaaagt acagcccggc gagacggacc ttgcgctgcc cgacccgctg
    19441 ccgttcattc tctcccgcac ctacagcagc taccggacga agacgcctgc gccagtggga
    19501 actttttggc cccggctgga aagcgccttc tgatatccgc ttacagatac gcgatgatgc
    19561 actggtactc aacgacaacg gcgggcggag cattcacttt gagccgctgc tgccggggaa
    19621 ggctgtgtac agccgcagtg agtcaatgtg gctggtgcgt ggcggtaagg cagcgcagcc
    19681 ggacggccac acgctggcac gactgtgggc gtcgctgccg ccggatatcc ggttaagccc
    19741 gcatctttac ctggcgacca acagcgcaca ggggccgtgg tggatactgg ggtgatctga
    19801 gcgggtgccg ggtgctgagg acgtactgcc agcgccgctg ccgccgtacc gggtgcttac
    19861 cgggatggcg gaccgcttcg ggcggacgct gacgtaccgg cgtgaggccg caggcgacct
    19921 ggccggggaa atcaccggcg tgacggacgg tgccgggcgg gagttccgtc tggtgctgac
    19981 cacacaggcg cagcaggcgg aagaggcccg gcagcaggcg gtcgtcgccg gagcaaaacg
    20041 accggacatt cctgattcac tgccggagag cacagagtac ggccccgaca ggggtatccg
    20101 cctttcggcg gtgtggctga tgcacgaccc ggcatacccg gagagcctgc ccggtgcgcc
    20161 actggcgcgg tacacgtata cggaagccgg tgaactgctg gcggtatatg accgcagcaa
    20221 tacgcaggtg cgcgctttca cgtatgacgc gcagcacccg ggccggatgg tggcgcaccg
    20281 ttacgcggga aggccggaga tgcgctaccg ctacgacgat acggggcggg tggtggagca
    20341 actgaacccg gcagggttaa gctaccgcta tctttatgag caggaccgca tcaccgtcac
    20401 cgacagcctg aaccggcgtg aggtgctgca tacagaaggc ggggccgggc tgaaacgggt
    20461 ggtgaaaaaa gaactggcgg acggcagcgt cacgcgcagc gggtatgacg cggcaggaag
    20521 gctcacggcg cagacggacg cggcgggacg gcggacagag tacggtctga atgtggtgtc
    20581 cggcgatatc acggacatca ccacaccgga cgggcgggag acgaaatttt actataacga
    20641 cgggaaccag ctgacggcgg tggtgtaccc ggacgggctg gagagcagcc gggaatatga
    20701 tgaaccgggc aggctggtat cggagacatc gcgcagcggg gagacagtac gctaccgcta
    20761 cgatgacgcg cacagtgagt taccggcgac gacaacggat gcgacgggca gcacccggca
    20821 gatgacggcg gttcaccgcg aggaaggtat cagcctttac cgccactatg acaaccgtgg
    20881 ccggttaacc tcggtgaaag acgcacaggg ccgtgaaacg cagtatgagt acaacgccgc
    20941 aggcgacctg actgccgtta tcaccccgga cggcaaccgg agcgagacac agtacgatgc
    21001 gtggggaaag gcggtcagca ccacgcaggg cgggctgacg cgcagtatgg agtacgatgc
    21061 tgccggacgt gtcatcagtc tgactaacga gaacggcagt cacagcgact tcagctacga
    21121 tgcgctggac cggctggtac agcagggcgg ctttgacggg cggacgcaac gttatcatta
    21181 tgacctgacc ggaaaactca cgcagagtga ggatgagggg cttgtcaccc tctggcacta
    21241 cgatgcgtcg gaccgcatca cgcaccggac ggtgaacggc gacccggcag agcagtggca
    21301 gtatgacggc cacggctggc tgagagaaat cagccacctg agcgaaggcc accgtgtcgc
    21361 tgtccactac ggctacgacg ataagggccg cctgacgggc gaacggcaga cggtggagaa
    21421 cccggagacg ggggaactgc tgtggcagca tgagacgaaa cacgcctaca acgaacaggg
    21481 gctggcaaac cgcgtcacgc cggacagcct gccgccggtg gagtggctga cctacggcag
    21541 cggttacctg gcgggcatga agctgggcgg gacgccgctg gtcgagtata cgcgggacag
    21601 gctgcaccgt gagacggtgc gcagcttcgg cagcatggca ggcagtaatg ccgcatatga
    21661 actgaccagc acatacaccc ccgcaggcca gttacagagc cagcacctga acagcctggt
    21721 gtatgaccgt gactacgggt ggaatgacaa cggcgacctg gtgcgcatca gcggtccgcg
    21781 acagacgcgg gaatacggct acagcgccac gggcaggctg gagagcgtgc gcaccctcgc
    21841 accggacctg gacatccgca tcccgtatgc gaccgacccg gcgggaaacc ggctgccgga
    21901 cccggagcta cacccggaca gcacgctcac ggcgtggccg gataaccgca tcgcagagga
    21961 tgcgcactat gtttaccact atgatgaata cggcagactg acggagaaga cggaccgcat
    22021 cccggcgggt gtgatacgga cggacgacga gcggacccac cactaccact acgacagcca
    22081 gcaccgcctg gtgttccaca cgcggataca gcatggcgag ccactggtcg agagccgcta
    22141 cctctacgac ccgctgagcc gccggacggg gaaacgggtg tggcgtcgcg agcgtgacct
    22201 gacggggtgg atgtcgctgt cgcgtaaacc ggaggtgacg tggtacggat gggacggcga
    22261 ccggctgacg acggtacaga ccgacaccac gcgtatccag acggtatacc agccggggag
    22321 cttcgcgccg ctcatccgca tcgaaacaga caacggtgaa cgggagaaag cgcagtgccg
    22381 cagcctggcg gagaagttac agcaggaagg gagcgaagac ggtcacggtg tggtgttccc
    22441 ggctgaactg gtggggctgc tggacaggct ggagggggaa atccgggcaa actgcgtgag
    22501 cagtgaaagc cgccagtggc ttgcgcagtg cgggctgacg gtggagcggc tggcggcaca
    22561 gatagagccg gtttatttgc ctgagcgaaa aatacatctt tatcactgtg accaccgggg
    22621 cctgccgctg gcgcttatca gtgaagacgg caatacggcg tggagcgcgg aatatgatga
    22681 atggggcaac cagcttaatg aggagaaccc gcatcacctg caccagccgt accggctgcc
    22741 ggggcagcag tatgataagg agtcggggct gtactacaac cggaaccggt actacgatcc
    22801 gttgcagggg cggtatatca ctcaggaccc gatagggctg gaggggggat ggagtctgta
    22861 tgcgtatccg ctgaatccgg tgaatggtat tgatccatta gggttaagtc ccgcagatgt
    22921 agcgctaata agaagaaaag
//
LOCUS       DAJVOY010000053        22607 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVOY010000053 DAJVOY010000000
VERSION     DAJVOY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.053"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(50..1699)
                     /gene="actP"
                     /locus_tag="OQL90_001816"
     CDS             complement(50..1699)
                     /gene="actP"
                     /locus_tag="OQL90_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1368619.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1696..2010)
                     /locus_tag="OQL90_001817"
     CDS             complement(1696..2010)
                     /locus_tag="OQL90_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCS1368620.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2142..4101)
                     /gene="acs"
                     /locus_tag="OQL90_001818"
                     /pseudo
     CDS             complement(2142..4101)
                     /gene="acs"
                     /locus_tag="OQL90_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4494..5930
                     /gene="nrfA"
                     /locus_tag="OQL90_001819"
     CDS             4494..5930
                     /gene="nrfA"
                     /locus_tag="OQL90_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1368621.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5975..6541
                     /gene="nrfB"
                     /locus_tag="OQL90_001820"
     CDS             5975..6541
                     /gene="nrfB"
                     /locus_tag="OQL90_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1368622.1"
                     /translation="MSVLRSLLTARVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6538..7209
                     /gene="nrfC"
                     /locus_tag="OQL90_001821"
     CDS             6538..7209
                     /gene="nrfC"
                     /locus_tag="OQL90_001821"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1368623.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7206..8162
                     /gene="nrfD"
                     /locus_tag="OQL90_001822"
     CDS             7206..8162
                     /gene="nrfD"
                     /locus_tag="OQL90_001822"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1368624.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8182..9900
                     /locus_tag="OQL90_001823"
     CDS             8182..9900
                     /locus_tag="OQL90_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1368625.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9893..10276
                     /gene="nrfF"
                     /locus_tag="OQL90_001824"
     CDS             9893..10276
                     /gene="nrfF"
                     /locus_tag="OQL90_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1368626.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10273..10869
                     /gene="nrfG"
                     /locus_tag="OQL90_001825"
     CDS             10273..10869
                     /gene="nrfG"
                     /locus_tag="OQL90_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1368627.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYVALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11210..12523
                     /gene="gltP"
                     /locus_tag="OQL90_001826"
     CDS             11210..12523
                     /gene="gltP"
                     /locus_tag="OQL90_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1368628.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12714..13402)
                     /locus_tag="OQL90_001827"
                     /pseudo
     CDS             complement(12714..13402)
                     /locus_tag="OQL90_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="OQL90_001828"
     CDS             complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="OQL90_001828"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15224..15226),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS1368629.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="OQL90_001829"
     CDS             complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="OQL90_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1368630.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGIAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="OQL90_001830"
     CDS             complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="OQL90_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1368631.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="OQL90_001831"
     CDS             complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="OQL90_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1368632.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20405..20680)
                     /locus_tag="OQL90_001832"
     CDS             complement(20405..20680)
                     /locus_tag="OQL90_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368633.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20889..>22607)
                     /gene="yjcS"
                     /locus_tag="OQL90_001833"
     CDS             complement(20889..>22607)
                     /gene="yjcS"
                     /locus_tag="OQL90_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCS1368634.1"
                     /translation="YKFDINAPAPETVNPSLWRQSQLNGISGLFKVTDRMYQVRGQDI
                     SNITFIEGDTGIIVIDPLVTPPSAKAALDLYFQNRPQKPIVAVIYTHSHTDHYGGVKG
                     IISEADVKSGKVQVIAPAGFMDEAISENVLAGNIMSHRALYSYGLLLAHNPQGNIGNG
                     LGVTLASGYPSIIAPNKTITKTGEKMIIDGLEFDFLMTPGSEAPAEMHFYIPALKALC
                     TAENATHTLHNFYTLRGAKTRDTSKWTEYLNETLDMWGNDAEVLFMPHTWPVRGNKHI
                     NDYIGKYRDTIKYIHDQTLHLANQGYTMNEIGDMIKLPPALANNWASRGYYGSVSHNA
                     RAVYNFYLGYYDGNPANLHPYGQVEMGKRYVQALGGSARVISLAQEANKQGDYRWSAE
                     LLKQVIAANPGDQVAKNLQANNFEQLGYQAESATWRGFYLTGAKELREGVHKFSHGTT
                     GSPDTIRGMSVEMLFDFMSVRLDSAKAAGKNISLNFNMSNGDNLNLTLNDSVLNYRKT
                     LQPQADASFYISREDLHAVLTGQAKMADLVKAKKAKIIGNGAKLEEIIACLDNFDLWV
                     NIVTPN"
BASE COUNT         5073 a         5955 c         6193 g         5386 t
ORIGIN      
        1 ctggagagga agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc
       61 ggccttgctc aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac
      121 gctcgcgcgc accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg
      181 ccacgctgat agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga
      241 ggatctgtac ccaaatagtc gggccgagga tcatcagcac cactgcggta atcaacccca
      301 gccagccgcc cagcatcgtg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa
      361 tgatcgggaa gttacagctc gccgcgatgg caaacgcaag gcctaccata aaggcgatgt
      421 tctgattctc aaacagcatg ccgaggataa tcgcaatcac acccaaaatc aggacggtga
      481 ttttcgatac ccgcagttct tcacgttcgg tcgcaccttt tttgaagacg ttagcgtaca
      541 agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc cgcaaccacc gcgaggatag
      601 tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca
      661 ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg
      721 gattcgcacc aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt
      781 agcccataaa cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg
      841 taaagaagcg catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca
      901 gagagagcgc ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt
      961 tcgggtgtac cgccatcgct tcgctgaaca gattattgaa gctaaagccg acgtgtttca
     1021 tcaccataaa ggccataaag ctggcaccga acagcaacag cacggcttta ataatttgca
     1081 cccaggtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     1141 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta
     1201 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     1261 gaatcggccc ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt
     1321 tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg
     1381 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aaaaatgagg
     1441 cggcggacat atagtcaccg gcaatcgcca gcccgttctg gaagcctgtg atattgccgc
     1501 ctgccgtgta gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga
     1561 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct
     1621 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     1681 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     1741 gaattcgccg ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac
     1801 tccaacacca attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag
     1861 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     1921 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     1981 ttctatccgc tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt
     2041 gttttaattc ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt
     2101 aggccggata aggcgtttac gccgcatccg gcaattgtgg gttacgatgg catcgcgata
     2161 gcctgcttct cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg
     2221 cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg
     2281 gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca
     2341 atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg
     2401 tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact
     2461 acggcggctt cggcaatctt cggatgcgcc accagcgccg actcaatctc tgccgtcccc
     2521 agacggtgac cggagacgtt cagcacgtcg tccacatgcc cggtgatcca gtaatagcca
     2581 tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag
     2641 gtctgttcaa aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg
     2701 gtgatcacca ggctgccttc ggtagcgcct tccagcgggt taccttcgtt atcgaccagc
     2761 gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagccc ggtagcgcca
     2821 ggcagtggcg tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccaccgga
     2881 catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg
     2941 cccacggaac cgagaatgcg cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct
     3001 tccgccatca gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg
     3061 tccaccacct gcgccatacg ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc
     3121 gtggtcgcac cgcaggccag cgggccgtac agcaagtaac tgtgtccggt cacccagccc
     3181 acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc
     3241 agcgccgcgt acaccagata accgccggta gtatgcagca caccttttgg cttaccggta
     3301 gaaccggagg tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc
     3361 tgatcgctcg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca
     3421 attttcccgc cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt
     3481 ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc cgcccggcac gcacaccttc
     3541 gtcggaagtg atcaccagtc gtgagttgga atcaataatg cgcccggcaa cggcttccgg
     3601 cgagaagccg ccgaaaatca ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc
     3661 aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt ttttaatgcc
     3721 cagctcgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt tatagctgat
     3781 atgtttgctc tggctggcgt cgtcgccttc ccagatgatg gcggtacgat cgccgttttc
     3841 ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct cgtaccattt
     3901 aatggacaca ttaccggggg caaaggaggt gtttttcacc ttctggtaag gtttgatcca
     3961 gtcaagaatt tttccctgtt cgccccagaa ggtatcaggt acgttaatag attgttgata
     4021 cattgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg caggaatggt
     4081 gtgtttgtga atttggctca tgcttttgtt ctccttgtag aatgttaata atatgtgaca
     4141 taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac agatcacgca
     4201 aaagtagaat tgtgcaaata aacggcaggg taatttttga aggtcagcaa caaaagttga
     4261 ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag atactgacta
     4321 actctaaagt ggtattttac atgcacttac aattgattaa agacaacatt cacagtgtgg
     4381 ttatttgtta cacatagggg cgagcaatgt catgacagtg taggtgcggt tactgtcgtg
     4441 aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa
     4501 ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt
     4561 ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt aactgtggaa gcgaagaatg
     4621 aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt
     4681 cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc
     4741 ccttctcgcg cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg
     4801 aaaccctgcg taccggtgcg ccgaaaaatg ctgaagatgg tccgctaccg atggcgtgct
     4861 ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact
     4921 tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact
     4981 gccataacac cgcctcacca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc
     5041 cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg
     5101 accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca
     5161 aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg
     5221 acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc
     5281 agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta
     5341 tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa
     5401 ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag
     5461 ctgccttgca aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg
     5521 ttgaagatca actggttcac gctcacttcg aagcgaaagc agcgctggat gcaggcgcga
     5581 cggaagctga aatgaagcca attcaggacg atatccgtca tgcccagtgg cgctgggatc
     5641 tggcgatcgc ttcccacggc attcatatgc acgcaccgga agaaggttta cggatgctcg
     5701 gtacggcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca
     5761 aaggcatcac ccatgaaatc cagatcccgg atatctcaac caaagagaaa gcccagcagg
     5821 ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc aaaacggtga
     5881 tcccgcagtg ggaagaacag gcacgtaaaa acggtctgtt aagccaataa ccccgttccg
     5941 cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac
     6001 tgctagggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg ctacccctgc
     6061 ggcgcaggca tctggtgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg
     6121 tctggactgt cataaaccag ataccgaagg tatgcatggc aaacatgctt ccgttatcaa
     6181 cccgaataac aaattgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca
     6241 ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg tcggagagca
     6301 gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt tctggccgca
     6361 cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga
     6421 tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga
     6481 tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg
     6541 acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc
     6601 gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat
     6661 gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcgaga agtgaacaag
     6721 gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct
     6781 gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt
     6841 gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt cgatgttaat
     6901 cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt
     6961 atccatccgg tcacgaagac ggcggacaaa tgcgatttct gccgtaaaac caatttgcag
     7021 gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat
     7081 ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac
     7141 aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg
     7201 agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg
     7261 ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac
     7321 gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc acgctgattg
     7381 tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg acaagaccgt
     7441 ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg atgggggtga
     7501 tgctgtttca gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg
     7561 atttgcttgc ccagcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga
     7621 gcctgctgac gcccgttcat cgcggactgg aaacgctgat gctggtgttg gcggtgctgc
     7681 tgggggctta taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc
     7741 cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga
     7801 tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcac tttgttcacc
     7861 ggatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc
     7921 tggcgctggg cgatgacggt aaagttcgtg cgctggtggc ggcattaggt ggcggtttct
     7981 ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgctca
     8041 aatcgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc
     8101 tggtcggcgt gttgatgctg cgctttttca ttctctacgc cggacagttg acggtggcgt
     8161 aagccagaaa agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgttg
     8221 ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg
     8281 cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcgcagtg cgcgctcctg
     8341 ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat
     8401 gtcgcccaac atagctacag cctgctgtcg tgggaactca aactggcggc ggtgtggggc
     8461 ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt
     8521 gcctggcatt atcggcagca aaccgatccg ctatttccgc tgacgctagc cgttttatct
     8581 ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt ccgatccctt cgtgcggata
     8641 tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc
     8701 tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg
     8761 ctggcgagtc tactgcgcgg cgagtttgat gcggccagcg cccgaatttg ctggcgctgg
     8821 gcgttacctg gctggagtgc attaacggcg gggatcatcc tcggttcctg gtgggcctac
     8881 tgcgaactcg gctggggcgg ctggtggttc tgggatccgg tggaaaatgc ctctttatta
     8941 ccctggcttt ctgccactgc gctgctgcac agtttatccc tgacacgcca gcgggggatt
     9001 tttcgccact ggtcgctgtt actggcgata gttactctga tgctgtcgct gctgggcacc
     9061 ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc
     9121 gccgtgccgt tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc
     9181 tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta
     9241 atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt
     9301 tatccgatga tttacggtct gctgggctgg ggacgcctct ccgttggcgc gccgtatttt
     9361 aaccgcgcga cgttaccgtt tggtctgttg atgctggtgg tgattgtgct ggcgacgttt
     9421 gtctctggca aacgcgtgca gcttccggcg ctggtagctc atgcgggcgt gctgttattt
     9481 gccgctggga tcgtggtttc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg
     9541 ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc
     9601 aaaggcaatt acaccagcga aaaagcgata gtggcactgt ttgaccatca gcaacgcatt
     9661 ggtgaactga tgccggagcg gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg
     9721 tcaattcgct ggaacggcat ccatgactgg tatgtggtca tgggtgaaaa aaccggagcg
     9781 gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga
     9841 ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaggaagcg cgatgaataa
     9901 aggacttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg tcgtagacac
     9961 ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg
    10021 ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat
    10081 gcgccatcag gtttacagca tggtggcgga ggggaagaac gaagtcgaaa tcattggctg
    10141 gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct
    10201 ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt
    10261 gagggcgaag cgatgaaaga gcccaaaata ccggtgaaaa tgctgacaac actcacgatt
    10321 ttgatggtat ttctctgtgt cggcagttat ctgttaagtc caaaatggca ggcggtacat
    10381 gcggaatatc agcgtcagcg cgatccgcta catcagtttg ccagccagca aaccccggaa
    10441 gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg
    10501 gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac
    10561 cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgtggcgct ggcgacggtg
    10621 ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc
    10681 ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg
    10741 caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc
    10801 cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga
    10861 tcggattaat catctggtga tgaagattat gttattgagc tgcataaaaa ataatcgaat
    10921 gaacatatgc caaaaataat cactaatcag tattattgca gattaaacaa ataaaaatct
    10981 ttccataaca aatggttatt cattaatcct gacataaact cgcgttatta tgcattaatg
    11041 cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat
    11101 aagcgttaaa aaaatctaca aaccaacgca acacaattca tgcctggcag tatgtcacgt
    11161 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat
    11221 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
    11281 ctacctgcac taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc
    11341 gggtgatatc ttcatccatc tgattaaaat gattgttgtg ccgattgtga tctccacgct
    11401 ggtggtgggt atcgcgggtg tgggtgatgc caaacagctc gggcgtattg gcgcgaaaac
    11461 cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa
    11521 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
    11581 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
    11641 gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
    11701 cttcttctcg gtgctgtttg gtctggggct ttcctccctg cccgcgacgc atcgtgaacc
    11761 gctggtgacc gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
    11821 gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
    11881 ctcttctctg tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt
    11941 cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat
    12001 tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtact
    12061 gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagcttcgt
    12121 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
    12181 tattttcatc gctcagctgt atggcattga cctgtccatc tggcaggaaa tcattctggt
    12241 gctgacgctg atggtgacct cgaaggggat tgctggcgtg ccgggcgtgt cgtttgtggt
    12301 gttgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgctgg
    12361 tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
    12421 ggtgctggtg attgccaagt gggaacacaa atttgaccgc aaaaaggcgc tggcttatga
    12481 gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccggggt
    12541 tatccacccg gcaatgtgtg gatgcctgat gcgacgctgg tgcgtcttat caggcctacg
    12601 ccagacagcg caatagcctg atttagcgtg attttgtagg tcggataagg cgtttacgcc
    12661 gcatccgaca tcaacgcctg atgcgacgct taacgcgtct tatcaggcct accttaaccg
    12721 ttggttaatt tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc
    12781 aaccagtgca gcgccttttg tttgttcttc tcgataaaac cttcttcacc gtttaagaac
    12841 atcattcccg cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa
    12901 tactaggttg ccttgtcatc gtcggcctta atacccacgc cgttggcgta aatcaaacca
    12961 agcagcattt gggcatcgac ggcagagtcg ttctccagat cttccgaggc ttttctaaca
    13021 gcgaaatcgc ctttggataa tccggtttac ccgcttgagt atttaccaga atatgcgcca
    13081 gcgttacttc accttcttta ctgcccgctt gcgccgcttt ctctgcaaga acttttgctt
    13141 gtggatagtc cagactgccc ggattggtga ttttgatctg cgccaacagc gcgcaggcat
    13201 cagcatcacc gctgtcggcg gctttctgtg cccaatattc ggctttgctc aaatcgccgg
    13261 agctaaacca gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc tctgctgcct
    13321 ttaaatactg gctgccaggc tcggagtcgt tggcgtgggc aaagaatgtc aaaaacaaca
    13381 tcaatgcgat aatttttttc attttgataa tacgattggg tagggagtaa ccagtataaa
    13441 gagttcgaat ggataaaaaa acagcctccg aaaggaggct gtagaaagga ctgtattacg
    13501 ccagtgccgc ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc acgtactgct
    13561 cggcggcgcg ctgatcggcg atcggctcga cgcgcacggc gcagtatttg tattccggcg
    13621 ttttcgtaat cgggcttaag ttttcggtaa ccagctcgtt acaggcacca atccaccact
    13681 ggtaggtcat gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca cgggtgataa
    13741 ttttgccttt acgcgagtgc acccaaacca atgcctcatc ttcaataccc agacgtttgg
    13801 cgtcttcggt attgatttgt gcgtagccag gttcatcagc cagcgccgcc agtgccgcac
    13861 agttaccggt catcgaacgg caagagtagt gaccaacttc acgcaccgtt gacagtacca
    13921 tcgggtactc gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg aagaactgcg
    13981 ccagaccgtt cggggtatca aacttctctt taaacagata agaagtcccc tgatcggcat
    14041 ctgaagtatc gcggcaaggc cactgaatga agccctgttc gcccattttc tcgtaagtcg
    14101 caccgtagaa atccgggcac agatgacgca actcatccca gatctcctgg gtgttgttgt
    14161 agtgcatcgg ataacccata cgggtggcga tttcactgat gatttgccag tccgttttca
    14221 gatcccattt cggttcaact gctttgaaga aacgctggaa gccacggtca gccgcagtaa
    14281 acacgccttc atgctcgccc cacgacgttg acggcaaaat gacatccgcc gccgacgcgg
    14341 ttttggtcat aaagatgtcc tgaacgatga ccagttccag atcttcaaag gctttacgta
    14401 ctgccgacag ctccgcatca gtttgcagcg gatcttcgcc cataatgtac gcggcacgca
    14461 cttcgccatg cgctgcgcgg tgcggcagct cgctgatgcg atagccggta tgagcaggca
    14521 ggctttccac gccccaggct ttggcgaatt tctcgcggtt agccggatct ttcacgtact
    14581 ggtatcccgg atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct
    14641 gaccacgaac cgggtttaca cccgcatgcg gcttaccgag gttaccggtc agcatcgcga
    14701 ggctggtcag agaacgcacg gtttccacgc cctggtagaa ctgggttaca cccatgcccc
    14761 acaggatggc ggcgcttttc gcctgggcat acatccgtgc cgcctgacga atctcactgg
    14821 cgctgacgcc ggtgatatct tcaaccgact ccggcgtgta gccttcaacg attttacgat
    14881 actcttcaaa gccttctgta cgggaagcga cgaacgcttt gtcgtacaga ttttcttcaa
    14941 taatgacatg gcccatcgca ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt
    15001 gcatgtcagc aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata attttcgccc
    15061 cgttacgttt agcgttaatt acgtgattcg ccatgattgg gtgggaatcc gccgggttgt
    15121 acccgaaaac gaacactaaa tcggtattat caatttcgtt aatagcattg ctcattgcgc
    15181 cattaccgac cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc
    15241 agtcaacgtt attggtacca ataacggcgc gcgcaaattt ttgcattaca tagttggttt
    15301 cgttacccgt accacgcgag gagccggtcg tctggatggc atccggaccg tacttctctt
    15361 tgatggcgct caggcgctcg gcaacgtaat tcagtgcctc atcccaggaa acaggttcga
    15421 gtttgccgcc acgctggcga cggatcatgg gggttttcag gcgcggggtc aggatctggg
    15481 tatcgttaat gaagtcccag ccataataac ccttcagaca cagggtaccc tggttggttt
    15541 tcccctgcgc tgcctccgcc cggacgattt tgccgttatc gacgaccagg ttgattttgc
    15601 aacctgatgc gcaatagggg caaaccgtga cgactttttt catcggtctc gctccagtta
    15661 atcaaatcac gcatacgcgc tctcgactac agtatgcatc ttttatgcca cattttattt
    15721 ggggtcattc cctgatatta cgggcactat ttattcaaaa ctctgacgaa aaacaggctg
    15781 tcgtcagttt tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt
    15841 ttattttttc tcgacgacgg gagccgcctg atacccgccg cccagcgatt tcatcaactg
    15901 aatgctgtgg atcacccggc ggctgtccag catcagtaat gacatctctt cggcaagcac
    15961 tggcaaccgg gcttcggtgg cctgtaagcg gctggttaag ccgcgctgat aggcggcctc
    16021 ggcagcgcgc tgggtgaagc gcgttgcttc cacgcgttca gcctgcattt ctcgttcgtc
    16081 gttgagcgtt tgcagacgcg tgccgttgac ggcaacgtca cgcaccgcgt tcagtactga
    16141 ctggttgtaa cgttcaatca tcatgttgct ggcggcgcga gtgccttcga gattggcatt
    16201 caaccgtcca ccgtcaaaca gcggcaattt cagacccggg atgaagttga actggcgact
    16261 ggtttttttg aataaggtat ccaggtggat ggagtccaga ccgaaaaacg ctttgatatc
    16321 aaagctcggg tagaacagtg cccgcgcgga atccacctga tctaatgacg cctgaacata
    16381 ccagcgcatg gcttgcagat ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg
    16441 aatgccggtc tggactcgtg gtaatgccac cggtttgatc tccggcatat cgctggcccc
    16501 cgcgccaatc aatgcacgca gagattctcg cgtttcggtg atttgccctt tgacggcagc
    16561 aatttgttta tcgaccgcca gaatctgtgc ccgcgcgccg tggaaaggca cttgtgcttc
    16621 cagaccgtgc gccactttac tctggtgcgc tttcaccgcg taatcaatca catcgcgagt
    16681 ttgttctaac agatcgagca tctgatagct ggcctgcata ctgtaataaa gctgcgctat
    16741 gcccgtggtc agcgatagct ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc
    16801 aatggcggcg gcaaccgctg agcgatgcac accccacaaa tcaagatcca gtccggcaaa
    16861 caaacctact gtggcttccg tatagtacgg cccgtccata cccagtgagg gcgcatccat
    16921 cgcataaggg cttaaaaagc cgttcgccga gacgcgttgg cggttgagca tccctaacgc
    16981 tgcgacctgt aattgtgaac cggcatctaa caaatcggcc tgggactgcg ctttttcttc
    17041 ccgcagtttc gcttcggcga gggtgtgtga accacttagc gtccgttgga tcagcgcatc
    17101 cagctgcggg tcattgagtt gtttccacca ctgcgcctgc ggccagccgg agctggcaag
    17161 atgaatatcg tcggccagtt tgatttgttc cggtttgagc tgttgatgag gtgcagaatc
    17221 cttacgtacc agggcacagc cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag
    17281 cagacgtgaa agttgacgat tgatcattgc gtggctcctt gtgcctgttc cgtggcgggc
    17341 gttaatgccg gggctgtcca gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc
    17401 tgttttaata aggtgggcag gatggcctgc gaggcgggtg tctcttcaag cgttatttga
    17461 ggagaacagc tgagtgcggt tgccagaccg gcagcgtatt tttccagggc gtcggcaaac
    17521 tgacctgctc tgtccggctg tagtgcgtta tccagcgcct gcgccgagtt ccaggtggca
    17581 tcccaggcgt gaagaagatc gcggccctga cgaataaccg tttgcgaacg ttcaatcagt
    17641 aaggcgcgtt cttcgctgtc cagttgacgc tccagcgcca cgcgttggca catctcttca
    17701 caggcattaa acgccgcatg cagacctata cgaatttgca gataagtgcg cagagccgtg
    17761 acttcctgct ggcgtgggat ccgcattact ttacttagca tacccagcgc accggctagt
    17821 ttttgcggca gagtgcgcgc ttcactttca ggccagacaa aggtgtaaat caccgcggac
    17881 accacggtac caatgatgat acccagggcg cgatcgcgaa tttccaccag gtcgtacact
    17941 gggccaaaaa cgttttcgag cgtggcgagc gcgaaggtga cgaccatctg tgtgccgata
    18001 taagaagagc gttcagagct ggtggcaatc catgcgccca acaggaaaat cggtgccagc
    18061 acaaacagca attcgacaat attgtccagc cagggcatga ccagtagcgt gaataacagc
    18121 gccagaatcg cgccgcaaaa ggccccgcca aaacgcagca ccatcttctg gtacgacgaa
    18181 ccgacatttg gattagcgac gatcacgcag gtcagcatac aggtgtgaat gccttcccag
    18241 tccacgccgc tgtaaaaggt gtaacagatc aaacaggcga gcaatgtttt taccgcgtag
    18301 cgtatatagt ctggattggt aaaagcatcg gcgaccattg atggcggttt ggcggcgggc
    18361 gttggcggcg tattcgggtt catctggccc agttgtaaca acgtctggca gatattctcc
    18421 aggttacatt cccgtgccgc caccgcttca ctttcactga tccgccagtc gctttgccag
    18481 cactgacctt cagcaacggc atgctgcagc ttgttgattt ctgaggctaa tttttgccga
    18541 aattcaataa ttgcctgaga atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag
    18601 taaatgtagg ttaccgttgc cacgcagctt tgccaccatg cgctttgagt tcgccagttg
    18661 gcatcgtccg cgagacaaaa gacattcagt ttttgcagcg ccagcgcctc tctttcaatc
    18721 cgcgtttcgg gtagcggtgc gaggctgtct gtcaggtgac taatggcatc atcaagccga
    18781 tcattaagcg cctgatgcat ttgcgaaatg gcacgactgg gaaaccacag cacgccgatt
    18841 aacgtcatca gcaaggttgg atagaggcca acaacgatac accacagcgt taagcgcacg
    18901 accacttccg gatagtcgag catggcgggg aaggtttgcc cgtaaatggc gacaatggcg
    18961 acagcgaaaa agaccagtcc cagccgatgg gtgcgcatca aaaacatgca gcccatcagg
    19021 atcggcccgg cgatgatcaa tcggatcaac ggttcgccgt atgaccattt gtagatcaaa
    19081 aacaggctgc cgatctccag caccgtggca accacaaaca agatcgccac aaatttggtg
    19141 taaaacgcgt tcgactgaat accgtaaaac agcactgcca gcgataacgc cacaaaaggg
    19201 atctcaaaag tcatcgagat cagaatcacc agcaggcagc ctacccagag ttgcacgatc
    19261 tgcggcacgc ggccgggtcg ccgctcgctt aactcttcat gaaagaacgc cagcagcctg
    19321 accaccggta atggcaggga gttgagcgcg ctcattattg cggctcaaga ttagcgactg
    19381 ccgaagcgcc gatgcggaac atttccgggt caggtttatc gaccatgatt ttgaccggaa
    19441 aacgctgggc aacgcggacc cagttaatag agcgagaaac tttcggcagg ccgcccagca
    19501 ccaggccgcc gtcattcggt agcacaccgt agccaatcga atccacttta ccctcgaagg
    19561 ttttgccgct gtcactcatc aggcgaatcg ttgcgggtgt acctgagcga atatttttca
    19621 gatcggtttc gcggaagttg gcgatcacgt accagtgacg agtgtcgatt agggtaaaaa
    19681 tagggcgcat ggcagaagca aattgcccga cggaggtttt gagggaaatg acccggccat
    19741 caaacggcgc gcgaacggtc gccatttcca gatgcagttt ggtcagggca atatccgctt
    19801 cgaccgccgc acgctgggca actaatacat ccacgccgct gacggcgctg gcggctgact
    19861 gcgcctgtaa caatacggca ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac
    19921 ggtcaacatc ttccgctgag acaaaacctt ctttcagtaa tggctcggtg cggcgtaatg
    19981 tatctgtggc ctgtttcgcg gcggcacgtg ctttttctac cgtggcatta accgagtcgg
    20041 caccaaactg ttgggcgtca acgctacgct gggtgagcat aatttgctta tccagcgccg
    20101 cgagggaggc ttcagctttc gccagattgg cttcgtacgg gcgcgggtca atgcggaaca
    20161 gcaaatcgcc ctgtttgact gcctggttgt cggtgaccgc cagttctaca atgcggccgc
    20221 tgacttccgg caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg
    20281 cactgtctac gcgccagata acgaaaacaa gggcaaccag cgccaacgca accactaaca
    20341 gagcagggaa tttactgcga ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa
    20401 tagattagaa aaatgccagc cagaacagca tggcgtagac agcaaaaagg gcggtataaa
    20461 taattccgac aaatgccaga ttgatatttg cccgctgaat aattcgcctc gtgattagcg
    20521 tcaaaataag gctggcaatg gcgcagaaaa accagctggg ataataagcg ccgatcaccg
    20581 ggattgccgg agataatgag catcccgaaa ccatgaagac gggaattatc caggctgttt
    20641 ttttgagttg catcgccatg cgagagagaa ctgttggcat attaaataca tcctgtttgc
    20701 tgcgaacaat atctgtagtg aatatcaata tccattgaag gcgtcagtat ggctattttc
    20761 ttgatgaata aaaatagctt tattcagcca ccgcattcat aacttatata ttttatgtgt
    20821 taattgattg tatgtatttt tataataaat gcttttgggg gcttaagcgc ccccaaaagt
    20881 gagtggcatt aatttggggt tacgatattc acccacaaat cgaaattatc cagacaggca
    20941 ataatttctt ccagtttcgc gccattgcca ataattttgg ctttcttcgc ttttaccaga
    21001 tccgccattt tggcttgtcc tgtcagcacg gcgtgcagat cttcacggct gatgtagaaa
    21061 gaggcgtcgg cttgcggttg cagtgtttta cggtagttaa gcacgctatc gttcagcgtc
    21121 aggttgaggt tatcgccgtt gctcatattg aagttcaggc tgatattttt acccgcagct
    21181 ttcgcgctat cgaggcgaac ggacataaag tcgaacagca tttcgaccga catcccgcga
    21241 atggtgtccg gggaaccggt ggtgccgtgg ctgaacttat gcaccccttc gcgcagctct
    21301 ttcgcgccgg tcaggtagaa accgcgccag gtggcggact cggcctgata gcccagctgt
    21361 tcaaagttat tcgcttgcag attcttcgcg acctggtcac ccgggttggc ggcaatcacc
    21421 tgtttcagca gttctgccga ccagcggtaa tcaccttgct tgttcgcttc ttgcgccagg
    21481 ctgatgacac gggcagaacc gcccagcgcc tgcacgtaac gtttacccat ctccacctga
    21541 ccatacggat gcaggttagc cgggttaccg tcgtaatagc caagatagaa gttatacacc
    21601 gcgcgggcgt tgtggctgac agaaccgtaa tagccacggc tggcccagtt attggcaagt
    21661 gcaggcggca gtttaatcat gtcgccgatt tcattcatgg tgtagccctg gttcgccagg
    21721 tgcagggtct ggtcgtgaat gtacttgata gtatcgcgat atttaccaat ataatcattg
    21781 atatgcttat tgccccggac cggccaggtg tgcggcataa acaggacttc cgcgtcgtta
    21841 ccccacatat ccagcgtttc gttcagatac tcggtccact tgctggtgtc gcgggttttc
    21901 gcgccgcgca gagtgtagaa gttgtgcagg gtatgcgtgg cgttctcggc ggtacacagc
    21961 gctttcaggg ccggaatata gaagtgcatt tcggctggtg cttcgctacc tggggtcatc
    22021 aggaagtcaa actccaggcc gtcgataatc atcttctcac cggttttggt gatggttttg
    22081 ttcggtgcga tgatgctcgg gtagcccgat gccagcgtta cgccaagacc attgccgatg
    22141 ttaccctgag ggttatgcgc cagcagcagg ccgtaggagt aaagtgcacg gtggctcata
    22201 atattacccg ccagtacgtt ttcgctgatg gcttcgtcca taaagccagc aggggcgata
    22261 acctgcactt taccggattt cacatcagct tcagagataa tgcctttcac gccgccatag
    22321 tggtcggtat ggctgtgggt ataaataacc gcaacaatcg gtttttgcgg gcgattttgg
    22381 aagtaaaggt caagggcggc ttttgcgctc ggtggcgtca ccagcgggtc gatgacaata
    22441 atcccggtat caccttcgat aaaggtgatg ttggagatat cctgaccgcg aacctggtac
    22501 attctgtcgg tgactttaaa caggccggaa ataccgttta actgcgactg acgccacagg
    22561 ctagggttaa cggtttcggg cgcgggcgca ttgatatcaa atttata
//
LOCUS       DAJVOY010000054        22445 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVOY010000054 DAJVOY010000000
VERSION     DAJVOY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.054"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            31..222
                     /locus_tag="OQL90_001834"
     CDS             31..222
                     /locus_tag="OQL90_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1368635.1"
                     /translation="MKYGKLGYIVPRPQKIRGRWLIDRRAVFVGPGETGIAPEIHTGD
                     DDALKEILTHVTEATKKQH"
     gene            191..1297
                     /locus_tag="OQL90_001835"
     CDS             191..1297
                     /locus_tag="OQL90_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1368636.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1477..2271
                     /gene="potC"
                     /locus_tag="OQL90_001836"
     CDS             1477..2271
                     /gene="potC"
                     /locus_tag="OQL90_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1368637.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDETKGNTGDVK"
     gene            2268..3314
                     /gene="potD"
                     /locus_tag="OQL90_001837"
     CDS             2268..3314
                     /gene="potD"
                     /locus_tag="OQL90_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1368638.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3470..4291)
                     /gene="cobB"
                     /locus_tag="OQL90_001838"
     CDS             complement(3470..4291)
                     /gene="cobB"
                     /locus_tag="OQL90_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1368639.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQEALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4307..5218)
                     /gene="nagK"
                     /locus_tag="OQL90_001839"
     CDS             complement(4307..5218)
                     /gene="nagK"
                     /locus_tag="OQL90_001839"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1368640.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5247..6491)
                     /gene="lolE"
                     /locus_tag="OQL90_001840"
     CDS             complement(5247..6491)
                     /gene="lolE"
                     /locus_tag="OQL90_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1368641.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6491..7192)
                     /gene="lolD"
                     /locus_tag="OQL90_001841"
     CDS             complement(6491..7192)
                     /gene="lolD"
                     /locus_tag="OQL90_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1368642.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7185..8384)
                     /gene="lolC"
                     /locus_tag="OQL90_001842"
     CDS             complement(7185..8384)
                     /gene="lolC"
                     /locus_tag="OQL90_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1368643.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8646..9719
                     /locus_tag="OQL90_001843"
     CDS             8646..9719
                     /locus_tag="OQL90_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1368644.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9847..13293
                     /gene="mfd"
                     /locus_tag="OQL90_001844"
     CDS             9847..13293
                     /gene="mfd"
                     /locus_tag="OQL90_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1368645.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13437..14399
                     /gene="ldtC"
                     /locus_tag="OQL90_001845"
     CDS             13437..14399
                     /gene="ldtC"
                     /locus_tag="OQL90_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCS1368646.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14482..14739)
                     /gene="bhsA"
                     /locus_tag="OQL90_001846"
     CDS             complement(14482..14739)
                     /gene="bhsA"
                     /locus_tag="OQL90_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCS1368647.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14980..15612
                     /gene="comR"
                     /locus_tag="OQL90_001847"
     CDS             14980..15612
                     /gene="comR"
                     /locus_tag="OQL90_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1368648.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15674..16213)
                     /locus_tag="OQL90_001848"
     CDS             complement(15674..16213)
                     /locus_tag="OQL90_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1368649.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16440..17744)
                     /gene="ndh"
                     /locus_tag="OQL90_001849"
     CDS             complement(16440..17744)
                     /gene="ndh"
                     /locus_tag="OQL90_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1368650.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(18144..18686)
                     /locus_tag="OQL90_001850"
     CDS             complement(18144..18686)
                     /locus_tag="OQL90_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1368651.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18709..19734)
                     /gene="nagZ"
                     /locus_tag="OQL90_001851"
     CDS             complement(18709..19734)
                     /gene="nagZ"
                     /locus_tag="OQL90_001851"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1368652.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19745..20569)
                     /gene="thiK"
                     /locus_tag="OQL90_001852"
     CDS             complement(19745..20569)
                     /gene="thiK"
                     /locus_tag="OQL90_001852"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1368653.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20553..21191)
                     /gene="lpoB"
                     /locus_tag="OQL90_001853"
     CDS             complement(20553..21191)
                     /gene="lpoB"
                     /locus_tag="OQL90_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1368654.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(21205..21582)
                     /locus_tag="OQL90_001854"
     CDS             complement(21205..21582)
                     /locus_tag="OQL90_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1368655.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21585..21944)
                     /gene="hinT"
                     /locus_tag="OQL90_001855"
     CDS             complement(21585..21944)
                     /gene="hinT"
                     /locus_tag="OQL90_001855"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1368656.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
BASE COUNT         5589 a         5892 c         5572 g         5392 t
ORIGIN      
        1 aaaccaaaac ggttcagtct cactaccctg gtgaaatacg gaaaactagg atacatcgtt
       61 ccaagaccac aaaaaattcg tgggcgttgg ctgatagatc gccgagcagt atttgttggg
      121 ccaggtgaaa cgggaattgc gccggaaatt catactggcg atgatgatgc actgaaggag
      181 attttaactc atgtcaccga ggccacgaaa aaacagcact gacgtagccg gtctttacga
      241 aaagtttgat cgcagaactg gcagagttta ctaccagtat aaaaatcctg tgactggaaa
      301 atttcacgga ctcggaacag acaaaggtaa ggcagaaaaa atcgcttcca cagccaatca
      361 gcgaatagct gcagcagaag ctgaatattt catgcgcaaa attgatgaaa gtccgtcagc
      421 aacaaaacgt cggggtatca gattaaaggc atgggttgat cgatatctga aaatacagga
      481 cacgcgactg aaaaatggag atattgcagc tacaactcac aaagaaaaaa ctcgaatggc
      541 tgcatacctg gtttcccgtc tgggaaacca cccattgaaa gaactggaag taagagactt
      601 tgcattaata ctggatgagt ggctggataa agacatggtc agcacagcga gagtaaatcg
      661 tggattatgg gttgatattt ataaagaagc acagcatgca ggggaagttc ctcctggatg
      721 gaatcctccg gaggctaccc gtaaaccgat ccctaaagta accagagcca ggctcaccat
      781 ggaagactgg caaaaaattt acaatgcaac gcctgaaaaa cactttatcc gtaacgcaat
      841 gcttcttgcg attgttactg gtcagcgccg tgatgacatt tgccacatgc gtttttcaga
      901 tgtgtggaac gaacacttgc atatcaccca gggaaaaacc ggaatgcgtc tggcgttacc
      961 gcttacacta cgctgtgatg ccattgggat aacgttaaaa gaagttattg atgggtgccg
     1021 agacagaata ttaagtccat atctaatcca tagtcggcac cagaaacaac cgaagccgat
     1081 gagtaaagac aacctgagcg actactttgc caaagcacgg gatctggctg ggataattcc
     1141 accagcagga aaaactccgc caacatttca tgaacaacgc tctctatcag aacggctgta
     1201 ccgtgcacag ggtatcgata caaaaacatt actaggacat aaagtccagg caaccaccga
     1261 tcgctataac gatactcgag gtcaggaatg ggtttaagct ggttatttga caaacaccat
     1321 atggaatatc cgtcaccatc tcaggaaaaa agtgttgtaa aatgcgtggc ctagttttgg
     1381 ggaaacgttt tggagagatt ttggagaaag gaaaaaattg aataaattca aaatcctgaa
     1441 agtgctccac cttatgaata agaaggtgga actcgaatga tcggtcgact gcttcgcggc
     1501 ggttttatga ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac
     1561 tcctttaaca gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc
     1621 ctgctgatga acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg
     1681 ttttcggcga cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat
     1741 cgctttcgcg gtaagccgtt cgttagcgga atgctgtttg tggtaatgat atcaccagat
     1801 atcgtgatgg caatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc
     1861 tggtcgctgc tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat
     1921 tcgcgcctga aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc
     1981 gaatttacaa ttctgcgaaa aatcatactg ccactggcaa tgccagcggt ggcagcgggc
     2041 tgggtgttaa gctttaccct gtcgatggac gatgtggtag tttcttcgtt cgtcaccgga
     2101 ccaagttatg aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg
     2161 gaagttaacg cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagt
     2221 cagcttattg ctcgtgatga aacgaaaggt aacacagggg acgttaaatg aaaaaatggt
     2281 cacgccacct gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg
     2341 acaacaacac gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac
     2401 agttcaccaa agaaacctgt attaaggtta tctattcgac ttacgagtcg aacgaaacca
     2461 tgtacgccaa gctgaaaacg tacaaagacg gtgcgtatga cctggtggtt ccttcaacct
     2521 attacgtcga taaaatgcgt aaagaaggga tgatccagaa gatcgacaag tcgaagttaa
     2581 cgaacttcag caatctcgat ccagacatgc tcaacaagcc ttttgacccg aataacgact
     2641 actccattcc gtatatctgg ggggcgacgg cgattggtgt taacggtgat gcggtggatc
     2701 cgaaatctgt caccagctgg gccgatctgt ggaagccaga gtacaaaggc agcctgctgt
     2761 tgaccgacga tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc tattccggta
     2821 acaccaccga tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa
     2881 acgtcgcagc gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc
     2941 tcggcatgat ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg
     3001 tggtgtggcc gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg
     3061 ccaaaaacaa agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa
     3121 aacaggttgc tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa
     3181 gtccagaagt ggcgaacgat aaaacgctct acccggatgc tgaaaccatt aaaaatggcg
     3241 aatggcagaa tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga
     3301 aagcaggacg ttaattcagc aagcgggcgg tggtattacc gcccgttttt tatttagcaa
     3361 ggatgtggct aatgagtcag gacagtagag ttttatccga ctctttcgtc ggtattgtgg
     3421 tccgctcgta ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct
     3481 tcagcaactt ttcaacaaac tcaggcacca cctggcttgc cgggccgtaa tatttctcgg
     3541 caaattcatt accgacctga cttggttcga gattcagttc cacggtgtgc gcgccatgca
     3601 gtttcgcttc gtgaacaaac ccagccgccg gataaacatg cccggaagta ccaatggcaa
     3661 tgaaaatatc agccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc
     3721 caaaccaaac gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt
     3781 ctggcgtaac gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa cgaactttca
     3841 gcaattcccc atgcatatga atgacattgg tattacctgc gcgttcatgc aggttgtcta
     3901 tattctgcgt taccagcaaa aagcgatcgc cgagagcctc ttgcagttta gccagcgcaa
     3961 gatgcgcggc gttaggctga atttttggct gctgcagctg tcgacgacgg gcgttataaa
     4021 acgcttgcac cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc
     4081 gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa
     4141 ttcctgcccc tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat
     4201 ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg gcgtttattt ttacgaaaac
     4261 gacttaaccg atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat
     4321 gtaggaaggc cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg
     4381 gaacacgagc tacaggtaag agatgacgag gcagcctgtc cgccagttgc gttgtgattg
     4441 ccgggaaatt cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga
     4501 tatttcccag acaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct
     4561 catcgccttg attataaagc gcaataattt cgggagcctg caactgttga tgataatagt
     4621 gttgatacag ccacgcaaaa ccgcgaccag acagataatt ctcaatgcag ccatgctgac
     4681 cacagccgca gcggcgtaac gggaaatcca accccatcat ggttaacgca tcaaccggca
     4741 gacgcatatg gccaaactcg ccggtaatgt agcttttacc ggtaatcggt ttgccgttga
     4801 aaatcagccc accgccaacg ccggtaccaa gaatcaaccc catcaccagt ggatattgcg
     4861 taaattcatc atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta
     4921 catcgcgatc aagacgtgcg ctcaggtcgg cacgcagcgg tttaccgctg gcagcgggga
     4981 cattggcggc atacagcgta ccatcttctg tttccggcat tcccggtata ccaatgccga
     5041 cagaacctct acagccaaaa cgttgatcag cttcggctac cagctcgcac actgcatcta
     5101 aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac
     5161 cgctatcaaa cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg
     5221 ccgctccttg ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata
     5281 ttactggcgc gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc
     5341 agtacgtaga agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaatagata
     5401 tcgctggaga ggaactgatg accgatcagc ttttcaatcc gctcaataat cggggtaagt
     5461 tgcagtgaaa caactacgcc gataatcagc ccacacaggc tgccgaacag ccctgccagc
     5521 aatccatacc agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat
     5581 actgcgatat cgccactctt atctttcacc gccatcacta aggtggagac gatgttgaaa
     5641 caggccacgc caatcaccag taccatcgcc agatacataa tggcgcggat catctggata
     5701 tcgcgataca tatagccgta agtaccaatc cagcttttaa tataaacata gctgttggtc
     5761 atttcacccg catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca
     5821 atacctgaca cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg
     5881 gcaaaactgt gatcgagttg gccactcaac tgcaaaatac ccgcaacgtg caaacgcaca
     5941 cgttttggct gcatcagttt atgctcagga ttcgagttgg ggatcatgat cgacacccaa
     6001 tcgccctgct tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg
     6061 cccgctttaa aattgcgcca cgcatccccc tgaacaaacg agggtaatgc gctcagacgc
     6121 tgttcctgtt gtgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc
     6181 accagcccgg tgaaattgat atacggcgca gcggcggcaa tacccggcac tttctgcact
     6241 ttatccagtg cttcctgcca gttagtccat ggctgattta ccgcttcgat ttcaccatgc
     6301 ggcaccactg ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag
     6361 ccgacgatca ataccgccac accgagggca atgccaatgg tagaaatgac ggagatcagc
     6421 gacaccatgc cgccgcgccg ccgtccgcga ctaaaacgca ggccaattaa taacgataaa
     6481 ggcatcgcca ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca
     6541 tttctaactg gcggctcata cgtttcgcca gttgcaggtc gtgagtaacc accaggaagg
     6601 cggtgccctg caagcgattc aattccccaa gcaactggaa gatgctgtcg gcgttacgcg
     6661 catcgaggtt accggtaggc tcatccgcca gtaccagacg cggattattg accagcgcac
     6721 gggcaatcgc tacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac
     6781 gatgctccag ccccaccgct tttaacatct ccagtgcacg gctgttgatt tcagcgggct
     6841 ttttcttgcc aatcagcagc ggcatagcca cgttttccag ggcggtaaaa tccggcagca
     6901 ggtggtgaaa ctgataaata aagcccagtt tctggttgcg cagttctgct tttgctgccg
     6961 acgacagttt gctcatcggt tgaccgttaa agatcacatc gccggaggtt ggcgtatcca
     7021 gtccgcccag caggtgcagc aaggtgcttt taccggaacc agagctaccg acgatcgcca
     7081 tcatttcacc ttcgccgacg ctgaaactga cattgtgcag tacatcggtt tgcacactgc
     7141 cttcctgata gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa
     7201 gcctcagcgg gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg
     7261 atagccatcg ccaccagcgc aataacaatg acctgtaaag gttcgatagc catcggcagc
     7321 gccgcgccat caagcaggac gccgattatt ggcatcagat tatttaactg gctggcaagc
     7381 agtgcgccaa gcgccgctcc gaggatcgca ccgataatcc cggcgctggc cccttgcacc
     7441 ataaagatca tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgctacttcg
     7501 ccctgcttct ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacggcg
     7561 acaatcaggc tcagcagtaa acccatcata tttttttcca tgcgtacggc ctggaacaac
     7621 tcgcctttac gatcacgcca gtcctgccat ttgctgtctt caggcagttt ttgctgactt
     7681 aatgagtcga ctttcagcgg ctcatccagc cacaaacgcc agccggtaat attgcctgcc
     7741 ggataacgca tcagacgcga ggcatcctca atattcacca gcatttcatt gccatcgact
     7801 tcactgttgg cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc
     7861 atcggcgtga actggctggc agatggtacc atcatgcgga tttgatcacc gcgattaacg
     7921 cctagctgtg aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca
     7981 gtttgtttca cattgaccag atacggcgtt agtggatctt tttgcgccgg atcgataccc
     8041 agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata
     8101 ggtgcgacgc gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta
     8161 agagagccat gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc
     8221 agctcgcgct caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg
     8281 gtaatgccga tggtagaaag ccaggagacg aaacgaccga agcgatccgc tgcacgccca
     8341 cgcatgtaac gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg
     8401 ctgtagcaaa gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag
     8461 tgatcgtcag ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatgtttt
     8521 atcatcaatc ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca
     8581 ttacacctcg ggtcataacc cgcaggatca accgcatgta aattaaaaaa gagcgtgttg
     8641 tcctgatgaa acaaaaagag ctatggatta accagatcaa agggttatgt atttgtccgg
     8701 tggtgattta tcactcggtc attacctttt atccgcatat gaccactttt ccgcatccgt
     8761 tatcggaagt cctgagcaaa tgctggatct atttcaatct ttaccttgca ccctttcgta
     8821 tgccggtttt tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt
     8881 ggggaaattg tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg
     8941 tggtgcagtg gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa
     9001 gcaatgcctc caatgccgct tatgccgatt ccaccggtga gttcctgcac gggatgatca
     9061 ccgccagcac cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa
     9121 tttttagccg tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta
     9181 atttcgttcc cacgccgtgg tggggaatga acagtgtgat ccgcaatttg ccttattaca
     9241 gccttggcgc atggtttggc gcaacaataa tgacctgtgt taaagaggta ccgttgcgcc
     9301 gccatctgct gatggcttct ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta
     9361 tctcgctgct gttgtcgctg gtatcgattg tggtgatcat gaagctgttt tatcagtacg
     9421 agcaacgatt cggtatgcgc tccaccagcc tgctgaatgt gattggctcc aacaccattg
     9481 ctatctacac cacccatcgc attctggttg aaatattcag cttaactctg cttgcgcaaa
     9541 tgaacgcagc acgctggtcg ccgcaagtcg aactgacact cctgctggtt tatccctttg
     9601 tcagtttgtt catctgtact gttgcgggct tgctggtaag aaaactttca cagcgtgcat
     9661 tcagcgatct gttgttctcc ccgccttcac tgcccgctgc cgtcagttac tcccgctaag
     9721 ccatcgcccc agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca
     9781 gcattgctta tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca
     9841 accgttatgc ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg
     9901 ctgggcgagt taaccggcgc agcctgtgca acgctggtag cggaaattgc cgaacgtcac
     9961 gccggtccgg tggtactcat tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa
    10021 atcagtcagt tcaccgatca gatggtgatg aatctggcgg actgggaaac tcttccctac
    10081 gacagctttt cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta
    10141 ccgacgatgc agcgtggcgt actgattgtt ccggtgaata cgcttatgca gcgcgtttgc
    10201 ccacacagtt ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga
    10261 gatgcattac gaacccaact ggacagcgcc ggttatcgcc atgttgacca ggtgatggag
    10321 cacggcgaat acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagctg
    10381 ccttatcgtc ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac
    10441 agccagcgca cgctggagga agtagaagcg atcaatctgc tgcccgcgca cgaatttccg
    10501 accgataaag cggcaattga actgttccgc agccagtggc gcgatacctt cgaagtgaag
    10561 cgcgatccgg aacatattta ccagcaagtg agtaaaggca cattacctgc cgggatcgag
    10621 tactggcagc cattgttctt cagcgaacca ctgccgccgc tgttcagtta tttccctgcc
    10681 aataccttgc tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt ccaggctgac
    10741 acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa
    10801 tcgctctggc tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgggtgcag
    10861 ctaaaaacta aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg
    10921 ccagacctag ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc
    10981 gagactttcg acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg
    11041 ggtgaactgc tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc
    11101 agcgaccgtg ggcgttatct gatgattggc gctgccgaac atggttttgt cgataaggtg
    11161 cgtaatctgg cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt
    11221 caggattctc gccgcaccat caaccccgat acactgatcc gtaaccttgc ggagctgcat
    11281 attggtcagc cggtggtcca tctggagcac ggtgtcgggc gctatgccgg aatgaccacg
    11341 ctcgaagcgg gcggcattac tggcgagtat ttgatgctca cctatgccaa cgacgccaaa
    11401 ctgtatgttc cggtgtcgtc actgcatctg attagccgtt acgcaggtgg cgcggaagaa
    11461 aacgccccgc tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg
    11521 gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa
    11581 gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt tgttctgcga cagcttcccg
    11641 tttgaaacca cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag
    11701 ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg
    11761 atgcgcgccg ctttcctggc agtagataac cacaagcagg tggcggtgtt ggtgcctacc
    11821 acccttctcg cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta
    11881 cgtatcgaaa tgatctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa
    11941 gtggcggaag ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc
    12001 aagtttaaag atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat
    12061 aaagagcgca ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg
    12121 atcccacgca cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg
    12181 ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc
    12241 cgggaggcga tcctgcgtga aattttgcgc ggggggcagg tttattatct ctacaatgat
    12301 gtggaaaaca tccagaaagc tgccgaacgg ctggcagaac tggtgccaga agcgcggatc
    12361 gccatcggtc acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat
    12421 catcaacgtt tcaacgtgct ggtttgtaca accattatcg aaaccgggat cgacatcccg
    12481 acagccaaca ccattatcat tgaacgcgcg gatcacttcg gtctggcgca gctgcaccag
    12541 ttacgcggtc gcgtcggacg ttcacatcat caggcatatg catggctgct gacgccgcat
    12601 ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat
    12661 ctcggtgcag gctttgcgct ggcaacgcac gatctggaga tccgcggcgc gggtgaactg
    12721 cttggcgaag aacaaagtgg ctcaatggaa accatcggtt tctcgctgta tatggagttg
    12781 ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc
    12841 agccagcaaa cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcattcct
    12901 gacgtgaata cgcgtttgtc gttctataaa cgtattgcca gcgccaaaac ggaaaacgaa
    12961 ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc tgctgccgga tccggcgcgt
    13021 accctgctgg atgttgcccg tctgcgccag caagcgcaga aactggggat caggaagctg
    13081 gaaggtaatg agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt taatccggcc
    13141 tggttgattg gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc
    13201 ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt
    13261 atgcgtgaac tggaagagaa cgcgatcgct taatggtaaa tcgggggcgt ttctgcgccc
    13321 ccatcggcaa catttacaaa ttctttgcac ttccctgcac tatccgacac tgtcaccatc
    13381 cataattcag gcttatctgt ttattacaat aaccttatat ttattatgga tttttggtga
    13441 tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct gccgtggcgc
    13501 tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc agcgggcagt cgtctggttg
    13561 gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc atcgccaaaa
    13621 aatataacgt cggctttctc gctctgttac aggctaaccc cggcgttgat ccttacgtac
    13681 cgcgcgcggg cagcgtgtta acgatcccgt tgcaaaccct acttccagac gcgccgcgcg
    13741 aaggcattgt gatcaacatt gcggagctgc gtctctatta ctacccgccg ggtaaaaatt
    13801 cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg acaccgacaa
    13861 tggtgaccac cgtttcagac aaacgtgcaa acccaacctg gacgccaacg gcaaacaccc
    13921 gcgcccgtta taaagcacag ggaattgagt tgcctgcggt agtgccggct ggaccggata
    13981 acccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat ttgcttcatg
    14041 gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt cgtctgcggg
    14101 atgacgatat caaaacactc tttagccagg tcaccccagg caccaaagtg aatatcatca
    14161 acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc
    14221 cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg aatagcgcaa
    14281 tgcaatcatt taaagatgca gcacaaactg acgctgaagt gatgcaacat gtgatggatg
    14341 tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa
    14401 tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca
    14461 ggcattaatg agggttaatg cttatttata aattactgct gttccatgga gggtattcgg
    14521 accggttaca gaagtaatac ggaaagattt tgcacccatc tcatccgctt tttgcgccag
    14581 ctgctcttcc agcgatccca gatttgtccc cgctttagca ctgattgtac cgactttttg
    14641 ttggccttct ggcgttgact gaacttcgac agccgcaaag ctggcaaatg acatggagct
    14701 taaaatcgcc gcagcgatga gggtttttac gtttttcata atagtggcct tatgcagatg
    14761 aatgacgtcg caagcaatta cttaacgatc gataactaaa tgacagatgt gatctggatc
    14821 acatacaaga tattttttat aacaatcatt aattaattta aaaatcttta aatttcagtt
    14881 aaatagaaag caattatttt tatgggatct ggactggtga acggaggtga ttatttttat
    14941 aataatcttt cattcacagt caccaggtac aacggcatca tggcaactga ctcaacacaa
    15001 tgtgtaaaaa aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag
    15061 gccatgaaat tgttctggca acacggttat gaagcgactt ctcttgcgga cctcgtcgaa
    15121 gcgaccggag ccaaagcgcc cacgctatac gcggaattta ccaacaaaga ggggttattt
    15181 cgtgccgttc tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc acagctgttt
    15241 tgtgaagaga aaagcgtgga gtctgcgctg gctgactatt ttgctgccat cgccaactgc
    15301 tttaccagca aagacacccc ggcaggctgc ttcatgatca acaactgcac caccctctcc
    15361 ccagattcag gagatatcgc caatacgttg aaatcacgcc atgcgatgca agagcgcact
    15421 ttgcagcagt ttttatgtca acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg
    15481 acacatctgg cagaattcct taattgtatt attcagggga tgtcgatcag cgcacgcgaa
    15541 ggtgcatcgc tggaaaaact aatgcagatt gccggaacga ctttgcgttt atggcccgaa
    15601 ctggtgaaat aagtcgcaaa aaaagcccct cagcctgagc gaatgagggg ccaaattgca
    15661 gagtacagcc ttgttatact ttgttattca aaatcagttg cccattacta tctagcggga
    15721 tctgcgtacc cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca atcttatagg
    15781 tcacatcata accgagcatt ttttctgact tgtcatacac cgttttacaa cgctgttgcg
    15841 tagtcgtgta agtatcgctt tcctggagag agccctggat ctggttaccg gcatatccac
    15901 cacccagcgc ccccacaaca gtggcgacat ctttaccgcg accaccgcca aactgatgcc
    15961 ctatcacgcc gccagcaaca gcgccgagca ccgacccggt aatgcgattt tcatcctgca
    16021 ccggtcgacg atgggtcact gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt
    16081 ccttgattgg ggttgcagaa acaacctgag cgtattgcgg gccacgttca aacacgttca
    16141 gactggccac tgccgctacg cccagcgcag ctgcgacacc aatcccgata cccgccaaca
    16201 ttgatttatt cacgggaaca cctccttctt tgcagttatg ccaatcatgc aagcgaagga
    16261 gaaaagcagc tatcagacag aagatgaaaa gcgcgggaat ggctggaaat acttaatgat
    16321 tcggaggagt ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc
    16381 tcgtaccatt tgtccagggc ggatgaggcg tttatgccac atccgccagt gtacgtcgat
    16441 taatgcaact tcaaacgcgg acggataacg cggttaatac tccccaccag catcattaat
    16501 ccggttttaa agtaaccatg cagcgcaatc tgatgcattc ggtatagcga gatatataca
    16561 aagcgcgcaa ttcgtccttc aatcatcatt gagccgcgcg tcaagttacc catcaggcta
    16621 ccaacggtgg agaagttcga cagcgatacc agtgaaccat gatctttata ctgataattt
    16681 ttcagcggct taccgtttat ctgcgccaga atattgttca ttgcgcaagt cgccatctgg
    16741 tgtgcagcct gagcacgcgg tggaacaaag cccccttccg gacgcgtgca tgacgcgcag
    16801 tcgccaatag cgtaaatgtc tggatcgtgg gtggtttgca gcgtcggttc caccaccagc
    16861 tggttgatac ggttagtttc aagaccaccg atatctttca ggaagtctgg cgctttgatc
    16921 ccggctgccc acaccatcag atcagcctca atatattcac catctttagt gtgcaggccg
    16981 ccttcatcag cactggtgac catggtttgc gtcagcacgc gaacgccaag tttcgttaac
    17041 tcactatggg ccgcagcaga gatgcgtggc ggtaatgcag gcaaaatacg ttctcccgct
    17101 tctactagcg ttacgttcag ggcttcgttg gtcagacctt tataaccgta gctgtgcagt
    17161 tgcttgaccg cgttatgcaa ctcagcggag agttctacac ccgtcgcgcc gccaccgaca
    17221 atcgcaatgt tcactttgcc attcgcgccc aggttggcgg agtatttcag gaacagattc
    17281 agcatctcct ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat gcagttctct
    17341 ttgacgcctg gcgtattgaa atcgttagag gtgctaccca gcgccatgac cagggtgtca
    17401 taggcgattt tacgttccgg aaccagcagt tcacctttct cgtcgcgtag ttctgcaata
    17461 gtgattgttt tcgcttcacg atcaatatca atgacggaac ccaactggaa ctggaaacca
    17521 tgattgcgcg catgggccag atagctcaac gcatcgacgc cttcatcaag cgagccagtc
    17581 gccacttcgt gcagcagcgg tttccacagg tggctgtggt tacgatcgac cagcgtaatt
    17641 ttggcttttt tcttacgtcc cagcttatgc cccagctgtg ttgccatttc cagcccacca
    17701 gcaccgccgc cgacaatcac aatctttttc aatggcgtag tcaacgtgac ccccttgaat
    17761 ttattaacca attgttaatt aatggttatt aacatagctc ttaattaaca acaggttaca
    17821 gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg gtgcattggt cataccaaaa
    17881 ttgatgttaa tcaagttttg ttgctgaaaa gataggcgac tttaccgggt gagatcaaaa
    17941 aatagagttt attgtttatt atttgagcga atttgtgaga gtgatataga gtgaggggtg
    18001 tatgcagggc gttcggtagg cctgataaga ggcgcatcag gcattgatgc tcgactgtcg
    18061 gatgcggcgt aaacgcctta tccgacttac agaattcgga gcgcatcagg taccggcaag
    18121 gttacctgat gcgacgaggg catttacccg agggttttga acgctttaat gcgctgcaaa
    18181 tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa
    18241 tgatgcaact cttcagatgt ccgctggctg ttaagcgctt catcattacg cgacaaaatc
    18301 accaggcaac gatcgcgatt cttctcgcgg aagttggtca cacacttagt ggcaatatcg
    18361 gcatactctt ccgggcgatc aatcttgcct tccatgttct cataagggaa caaattaggg
    18421 ttgaagatca cctggcggat atcgcaaaga aaaccaatcc gttccgccca gtatccgccc
    18481 agaccaacgc cgcaaatcag cggacgctcg tcgacgttca gttgcaacat tttgtccact
    18541 tctttaagca gatgctgcat atcatgtttc ggatgccgcg tactgtagct tatcaagcgt
    18601 acatccgggt caataaactg caattgtaag actttctcgt ggttacccgg actgttagag
    18661 tcaaaaccgt gtaaatagat aatcatcgct tcctcacata agccagggtt agtgacctgc
    18721 tttctcttcc tgccagcgtt catgtaactg attcagacgg gtgctgctcg ccttccagcg
    18781 agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg
    18841 ttctgccttg atcggtgaca gattatctaa cacgctgacg gcccctttac gattattgca
    18901 gaccaggatc atatcgcaac ctgcgtccag tgatgcctgc ccgcgttcgg cataactgcc
    18961 cataatcgcg gcaccttcca tcgataaatc gtcagagaaa attacaccgt caaaacacag
    19021 ttcctgacgc aaaacggttt tcagccagta agaagaaccg ctcgccggac gcggatcaac
    19081 atcactgtag atcacatgcg caggcataat ggcgtcgagt ttattttcgc ggattaacgt
    19141 gctgaaaacc gacatatctt tggcacgaat ttccgcttgc gggcgtgggt cgcacggcgt
    19201 ctctttgtgt gaatctgcag ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt
    19261 cattccggct tcatgcatcc catcaataaa ccgactagcg attgccaggg ctttttctgg
    19321 gtcggcatga taagaacgct cgccaatcgc cgcgctgata tgtcctacat ccagcactgg
    19381 cgcaaagctg atatcaatat ccatagcgat catttcgctg gccatcagcc aacccgcctc
    19441 ttgcgccagt ttgccgccct cttccattcc caacagcgca gcaaaggatt gtgctgccgg
    19501 taagcgggta aaaccttcgc gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac
    19561 caccagatga ttgcgcgatg ctgcgcggat ctggcgcacc agttcacgta actgggcagg
    19621 atcatgatag ttacgcgtaa agagaatcag ccctcccacc agcggatgcg ccagtatttc
    19681 acgctcttcc gcgtccagtt cataaccttc gacatccaac attactggac ccacactgct
    19741 ctccttattg ttttattaat agctgccgcc aggtgtcatc ggccagcctg ataaattgtt
    19801 gatcgccggt ttgtcgccag cggtactcaa accaccctgc tttgagcatc agcagccagg
    19861 gaaaccatcg cctgacctga cgccataatt gcgccggata aatcttcgcg cgagtggcat
    19921 agtcattgac caattgccgg tgctgttcag tattttccac ccacaccgcc gccagttcca
    19981 gcgcggtatc accatctccg gcatactccc agtcgatgag ttttaaccct gatgcgctat
    20041 gcactaaatt tccggcgtgg acatccatat gcaatggact taagcgtaaa ggtcgtggtt
    20101 cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cacagtccgc cgcgccggat
    20161 cgctttgctg ccagtacagt tccagtaacg gcaacagcgt tattcgccag ccaaaacgtg
    20221 gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc aaatacgttt
    20281 ttaccgcgcc gggcagatag tcgactacca tccagtcacg gagatataaa tgcggcttcg
    20341 gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac gcggactgcg
    20401 gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt tcaatgagaa
    20461 aactaccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc
    20521 gcgacagcaa ttcgtcgcgc gtgatgggat tattactgcg aaacggcacc tttacctgac
    20581 cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg agcgttaacg
    20641 ttgccagagg cgctggagta cagcacgtaa tgagcgccga cattgcgggc aatgcctatg
    20701 gctttactac gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg cttagccatc
    20761 gacagttgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc atttcgcagc
    20821 gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac gctatcaacc
    20881 agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac tatcggctgc
    20941 attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt tcgatcttcg
    21001 tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt ggggacaggc
    21061 tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg tgcaggttca
    21121 cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc gtagcgactc
    21181 atttttgtca tcaagattca ccccttacaa atatagataa agtctgactt tgtgcgcccc
    21241 cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg
    21301 tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg
    21361 aacggtaacg ggtttttgcc tttcgttata aagcgttgag gatgctgaag gttgaatatc
    21421 agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat
    21481 caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag
    21541 taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc
    21601 gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct
    21661 tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct
    21721 tgctcagcaa tttttgccac tacggtgatc atgcgtccca gcgcctgctc atgctcagct
    21781 gagacgtcgt tcacagtcgg aatgaggata ttcggaatga tcagaatatg cgttggcgct
    21841 tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg
    21901 atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc
    21961 aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg
    22021 agttaaacct gctctttcaa ccttaactca cgatttctta agcaaaaaat cgttttgctg
    22081 ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg
    22141 tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttac aaacaaaatt
    22201 attcgcacac taaatataat cgctcatcac cctcctccgg catgagcctg acgacaacat
    22261 aaaccatgag atttcaaatg ctttcaacac aatttaacag ggataatcaa tatcaagcca
    22321 tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct tctgccgctt
    22381 ttgctgcatc agccactgaa gaaacggtga ttgttgaggg ttcagccaca gctccagatg
    22441 atggc
//
LOCUS       DAJVOY010000055        22160 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVOY010000055 DAJVOY010000000
VERSION     DAJVOY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.055"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            76..658
                     /locus_tag="OQL90_001856"
                     /pseudo
     CDS             76..658
                     /locus_tag="OQL90_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
     gene            complement(753..2330)
                     /gene="guaA"
                     /locus_tag="OQL90_001857"
     CDS             complement(753..2330)
                     /gene="guaA"
                     /locus_tag="OQL90_001857"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1368657.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2399..3865)
                     /gene="guaB"
                     /locus_tag="OQL90_001858"
     CDS             complement(2399..3865)
                     /gene="guaB"
                     /locus_tag="OQL90_001858"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1368658.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4027..5397
                     /gene="xseA"
                     /locus_tag="OQL90_001859"
     CDS             4027..5397
                     /gene="xseA"
                     /locus_tag="OQL90_001859"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1368659.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5394..5609)
                     /locus_tag="OQL90_001860"
     CDS             complement(5394..5609)
                     /locus_tag="OQL90_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1368660.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5678..7150)
                     /gene="der"
                     /locus_tag="OQL90_001861"
     CDS             complement(5678..7150)
                     /gene="der"
                     /locus_tag="OQL90_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1368661.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7268..8446)
                     /gene="bamB"
                     /locus_tag="OQL90_001862"
     CDS             complement(7268..8446)
                     /gene="bamB"
                     /locus_tag="OQL90_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1368662.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8457..9077)
                     /gene="yfgM"
                     /locus_tag="OQL90_001863"
     CDS             complement(8457..9077)
                     /gene="yfgM"
                     /locus_tag="OQL90_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1368663.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9095..10369)
                     /gene="hisS"
                     /locus_tag="OQL90_001864"
     CDS             complement(9095..10369)
                     /gene="hisS"
                     /locus_tag="OQL90_001864"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1368664.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10480..11598)
                     /gene="ispG"
                     /locus_tag="OQL90_001865"
                     /gene_synonym="gcpE"
     CDS             complement(10480..11598)
                     /gene="ispG"
                     /locus_tag="OQL90_001865"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1368665.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11625..12638)
                     /gene="rodZ"
                     /locus_tag="OQL90_001866"
     CDS             complement(11625..12638)
                     /gene="rodZ"
                     /locus_tag="OQL90_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1368666.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12923..14077)
                     /gene="trmG/rlmN"
                     /locus_tag="OQL90_001867"
     CDS             complement(12923..14077)
                     /gene="trmG/rlmN"
                     /locus_tag="OQL90_001867"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1368667.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14227..14658)
                     /gene="ndk"
                     /locus_tag="OQL90_001868"
     CDS             complement(14227..14658)
                     /gene="ndk"
                     /locus_tag="OQL90_001868"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1368668.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14807..17119)
                     /gene="pbpC"
                     /locus_tag="OQL90_001869"
     CDS             complement(14807..17119)
                     /gene="pbpC"
                     /locus_tag="OQL90_001869"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCS1368669.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17120..22081)
                     /gene="yfhM"
                     /locus_tag="OQL90_001870"
     CDS             complement(17120..22081)
                     /gene="yfhM"
                     /locus_tag="OQL90_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCS1368670.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPANALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPSDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPLVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGDEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
BASE COUNT         5276 a         6217 c         5492 g         5175 t
ORIGIN      
        1 accacaacgt ataacgaaac gcgtattcag ccaaatacta aatgtcctga aaagagctaa
       61 taatcaggag gagtcatgaa gaaagttttt ctttgcgcca tcttggcctc cttaagctat
      121 ccggctatcg cctcatcatt gcaggatcaa ctctctgctg tagcagaagc ggaacagcaa
      181 ggtaaaaatg aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc
      241 cagcaagaga agcaacgtcg cgcaaatgcc caggtcgccg ctaacaaaag agcggcaacg
      301 tcgcgcaaat gcccaggtcg ccgctaacaa aagagcggca acggcagcag caaataagaa
      361 agctcgtcag gataaactgg acgccgaagc ctctgcggac aaaaaacgcg atcaaagtta
      421 tgaagatgag ctacgcagct tagagattca gaaacaaaaa ctggcgctgg caaaagaaga
      481 agcccgcgtt aagcgagaaa acgaatttat cgatcaggaa ctgaagcaca aagctgcgca
      541 aaccgatgtg gtgcaatctg aagctgacgc caacagaaat atgactgaag gcggtcgcga
      601 tctgatgaaa agcgtgggca aagcagaaga gaacaaatcg gacagctggt ttaattaatc
      661 gatgttagta acttcaagcc tatgattctt gaagataaaa aaccctctgt agtaacagag
      721 ggttttgttc attcatagtg cagggattaa aatcattccc actcaatggt agctggcggc
      781 ttgccgctga tgtcatacac cacgcgggaa ataccgttca cttcattgat aatgcggttg
      841 gaaacgcgac cgaggaaatc gtacggcaga tgcgcccagt gtgcggtcat aaagtcgatg
      901 gtttcgacag cacgcagaga gacaacccag tcatacttac gaccatcgcc cattacgcca
      961 acggaacgta ccggcaggaa cacagtgaac gcctggctga ctttgttgta caggtccgct
     1021 ttacgcagtt cttcaatgaa gatggcgtca gcacggcgca gcaggtcaca gtactctttc
     1081 ttcacttcac ccagcacacg aacgccaagg cctggtcccg ggaacgggtg acggtacagc
     1141 atgtcgtacg gcaggcccag ctccagacca atcttacgca cttcgtcttt gaacagctct
     1201 ttcagcggtt caaccaggcc catcttcatc tctttcggca ggccgcccac gttgtggtga
     1261 gatttgatga cgtgtgcttt accggttgca gaagccgcag attcgataac gtcagggtag
     1321 atggtgccct gcgccagcca cttcacgtct tccagtttca gcgcttcttc atcgaacact
     1381 tcaacgaaaa cgcgcccgat gattttacgt tttgcttccg gatcgttttc gccagccagc
     1441 gctgacagga agcgatcttc tgccggtacg tgaacaatgt tcagaccaaa gtgatcgcca
     1501 aacatatcca gaacctgctc tgcttcgttg agacgcagca ggccgttgtc gacgaatacg
     1561 caagtcagat ttttaccgat agcgcggtgc agcagcattg cggttacgga ggaatccaca
     1621 ccaccagaga ggccgaggat gactttatcg tcgcctacct gctcgcggat gcgagctaca
     1681 gcatcgtcga taattttcgc tggcgtccac agggcttcac actggcagat atcacgcaca
     1741 aaacgctcca gcatgtgcat accctggcgg gtgtgagtca cttccgggtg gaactgtacg
     1801 ccatagaagc gtttttcttc gttagccata atggcaaacg ggcagctttc ggtgctggct
     1861 acggtgacga agtcggacgg gatagcggta actttatcgc cgtggctcat ccagacatcg
     1921 agcagcggtt taccgtctgc ggtcagcgca tcttcgatac cgcgaaccag tgcgctgtcg
     1981 tttacgactt caacctgcgc gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg
     2041 cccaactgca ttgccatggt ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct
     2101 tcaaagacat actgcggcgc acgcggactg ttttcttcag tagtactttc cgggccgccg
     2161 gaaagaataa tgccgcttgg attgaagtca cgaatttgtg cttctgtcac atcccacgcc
     2221 cacagttcgc agtaaacacc cagctcacgc acgcggcgcg caaccagttg agtgtactga
     2281 gaaccgaagt ccagaatgag gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat
     2341 tccgaggcaa gtgaaacaga taatataaat cgcccgacat gaagtcgggc gaagagaatc
     2401 aggagcccag acggtagttc ggggactctt tagtaatggt cacgtcgtga acgtggcttt
     2461 cctgaatgcc cgcaccgctg atacgtacaa actccgcttt agtacgcagt tcgtcgatag
     2521 taccacagcc ggtcaggccc atacaggagc gcaggccgcc catctgctgg tgaatgatct
     2581 ctttcaggcg acctttatag gctacgcgac cttcgatacc ttccggcacc agtttgtcgg
     2641 cagcgttgtc actctggaag taacggtcag aagaaccttt ggacatcgca cccagagagc
     2701 ccataccacg gtaagatttg taagaacggc cctggtagag ttcaatttca cccggagatt
     2761 cttcagtacc cgccagcatg gaacccacca ttaccgcgct tgcgccagcg gcgatagctt
     2821 tggcgatgtc gccggagaag cgaataccac catctgcgat aaccggaata cctgtgcctt
     2881 ccagcgcttc aactgcgtca gcaacggcag tgatctgcgg aacacctacg ccagtaacga
     2941 tacgagtcgt acagatagaa ccagggccga taccgacttt aaccgcgctg caaccagctt
     3001 ctgccaaagc gcgtgcacct gcagctgttg ccacgttgcc gccgatgatt tgcagatccg
     3061 gatatttagc acgggtttca cggatacgct gcaaaacgcc ttctgagtgg ccgtgggagg
     3121 agtcgatcag cagaacgtca acgccagcgg caaccagcgc gtcaacacgc tcttcgttac
     3181 ccgcacctgc gccaactgca gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt
     3241 tcggtttacg ttccgctttc tggaagtctt tcacggtgat catgccgatc aggtggaatt
     3301 cgtcatcaac caccagcgct ttttcaacgc gtttttcgtg catttttgcc agcaccactt
     3361 cacgggcttc accttcacgc acggtgacca gacgctcttt cggcgtcatg taaacgctaa
     3421 ccggctggtt caggtcggta acaaaacgca cgtcacgacc ggtgataata cccaccagtt
     3481 cgttttcttc ggtaacaacc ggatagcccg caaaaccgtt acgctcggtc agttctttca
     3541 cttcgcgcag cgtcgtggtt ggcagaacag tctgcggatc agtcaccaca ccagattcgt
     3601 gttttttcac acggcgaact tcttctgcct ggcgttcaat ggacatgttt ttgtggataa
     3661 agccgatacc gccttcctga gccagagcaa tagccaggcg cgcttccgtt acggtatcca
     3721 ttgctgcgga aagcataggg atattcagac gaatagtttt cgtcagctgg gtgctgaggt
     3781 cagcagtatt cggcagaacg gtagaatgag caggaacgag gagaacgtcg tcaaacgtca
     3841 gagcttcttt agcgatacgt agcatgggca atatctcgac cagagtggtt aataaatatt
     3901 gccgcggcat tatacagagc gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg
     3961 ctatccccga agggcgggtt actatcgact gaataacctg ctgatttaga atttgatctc
     4021 gctcacatgt taccttctca atcccctgca atttttaccg ttagtcgcct gaatcaaacg
     4081 gttcgtctgc tgcttgagca tgagatggga caggtttgga tcagcggcga aatctctaat
     4141 ttcacacaac cggcttccgg tcactggtac tttacactca aagacgacac cgcccaggta
     4201 cgctgcgcga tgttccgcaa cagcaaccgc cgggtgacct tccgtccaca gcatggacaa
     4261 caagttttag ttcgcgccaa tattacgctc tacgagccgc gcggtgacta ccagataatc
     4321 gttgagagta tgcagccggc cggtgaaggg ctgctgcaac agaagtacga acagctcaaa
     4381 gcgaagttgc aggctgaagg tttgttcgat cagcaataca aaaaaccact tccctcccct
     4441 gcgcattgcg ttggtgtgat cacctcaaaa accggtgctg cgctacatga tattttgcat
     4501 gtgttaaaac gtcgcgatcc ttctctaccg gtgatcatct accccaccgc cgttcagggc
     4561 gatgacgcac cggggcaaat tgttcgcgcc attgaactgg cgaatcagcg caatgagtgc
     4621 gacgtgttga tcgttgggcg cggcggcggt tcgctggaag atttatggag ttttaacgac
     4681 gaacgcgtag cgcgggcgat ttttgccagc cgcattccgg tcgtcagcgc cgtcgggcat
     4741 gagacggatg tgaccattgc cgattttgtt gccgatctgc gtgcgccaac gccgtctgcc
     4801 gccgctgaag tagtgagccg taatcagcaa gagttactgc gccaggtgca atcgacccgt
     4861 caacggctgg agatggcgat ggattattat ctcgccaacc gcacgcgtcg ctttacgcag
     4921 atccatcacc gattacagca acagcatccg cagctccggc tggcacgcca gcaaaccatg
     4981 cttgagcgcc tgcaaaagcg gatgagcttt gcgctggaaa atcaacttaa gcgtaccggg
     5041 caacagcagc agcggttaac acagcggctg aatcagcaaa atccacagcc gaagattcat
     5101 cgcgcgcaaa cgcgcattca gcaactggaa tatcgtttag cagaaaccct gcgcgtacag
     5161 cttagcgcca cgcgtgaacg tttcggtaat gcagtaacgc acctcgaagc cgtaagccca
     5221 ctgtcaacgc tggcgcgtgg atacagcgtt actactgcta ctgacggcaa tgtactgaaa
     5281 aaagtgaagc aagttaaagc gggtgaaatg ctaaccacac gtctggaaga cggctggata
     5341 gaaagtgaag ttaaaaacat ccagccagta aaaaaatcgc gtaaaaaggt gcattaagcc
     5401 agcacgaatt caacgcgttt tttcgatatc aagccatgcc cgtgctgaca gaagtaatct
     5461 actgcaccgc aggcttttaa cacctgcaac ggttgatggc agtctggaca aagagctttc
     5521 atttcaataa tttccccgca ggatgggcaa cgcgcatggc cgttatcctg atcaagcaca
     5581 tgctgacatt gtggacaatg cagttccata tgaattcctc cggtagccat ccctctacat
     5641 tcatagaggg aatggcagat aaaatactta cggataatta tttatttttc ttgatgtgct
     5701 tcatcagacg cttacgttta cgcatctggg ttggcgtcag ggtgttacgc ttgttcgcat
     5761 acgggttttc cccttctttg aactgaatac gaatcggcga tcccattacg tccagcgatt
     5821 tgcggaagta gttcatcaag tagcgcttgt aggaatcagg caggtctttc acctgattac
     5881 cgtgaatcac cacaatcggc gggttataac caccagcgtg ggcatatttc agcttcacac
     5941 gacgaccgcg taccagcggc ggttggtgat cttcaacagc catcgtcatg atgcgcgtca
     6001 gcatagaggt ccccacacga cgggtggagc tgtcatacgc ttcacgtact gattcaaaca
     6061 agttaccaac accactgccg tgcaaggcag agataaagtg cacacgagca aaatcgataa
     6121 agcccagacg gaagtccagc gtttctttca cctgctcttt cacttcctgg ctcaggccat
     6181 cccacttatt caccacaatg acaagtgagc gcccactatt gagaataaag cccagcagcg
     6241 agagatcctg atcggaaata ccttcgcgcg catcaatcac taacatcacc acgttggcat
     6301 cttcaatggc ctgcaacgtt ttgattacgg agaatttctc tacagcatcg gtgattttgc
     6361 cgcgtttacg tacgccagcg gtgtcaatga gcacatactc acgtccatcg cgttccattg
     6421 gaatgtagat actgtcacgc gtcgtgccag gcatgtcata aacaacaacg cgctcttcac
     6481 caagaatacg gttagtgagt gtagacttac ctacgttcgg acgacccaca atcgccagtt
     6541 tgatcggcag actttgcggg tcgaagtcgt cttcctcttc ttcttcgccg ttctcttcca
     6601 cttcaaattg cgcccagtat tcagcgtctt cgtcgacttc ctcttgcggt gcgagatctt
     6661 ccatccacgg cagcagcaca tgctccagca gacttaatac gccacgaccg tgagacgcgg
     6721 cgatcgggta gatttcacct aagccaagcg agtagaaatc aaccactgcc tgatcgggat
     6781 ccagaccgtc agttttgttt gccaccagga aggtcggttt ttcacgggag cgcagatgtt
     6841 tggcaatcgc ttcatctgcc ggcatcaggc ccgcgcgcgc atccaccata aacagtacga
     6901 cgtccgcttc ttcaatcgcc agcagcgact gttccgccat gcgggtttct acaccgtctt
     6961 ctgtgccatc aatcccgccg gtatcaatac agataaactc acggccttca atttccgcac
     7021 gaccgtactt acggtcacga gtcagacccg ggaaatccgc aaccagcgca tctcgggtgc
     7081 gagttagacg gttaaataac gtggattttc ctacgttagg gcgcccgaca agcgcgacca
     7141 caggtaccat gtttaaagcc tcatttttat aaatcatcag acaacgcacg ctatattcgc
     7201 gtcgttgtta aaaacaggaa aacggcccct gtccaggagc cgttttcaaa gtgaacgaca
     7261 gagacgatta acgtgtaata gagtacacgg ttccgtcttt tgcctggatc agcagtttgc
     7321 cgtcagcggc aaccggttca gtctggaaac cggaactatc aactttttgc tgggcaacga
     7381 aacgaccatc ttcgacatta atccagtgca gataaccttc actgtcaccg accaccaggt
     7441 tgccatcata cagcaccgga gaagtcagca ggcgatgcaa cagatcgctt tgtgtccaca
     7501 gcgtaacgcc gccatcaatg gtcaacgcca tcacccggtc attttgatcg accagataga
     7561 tgcgattgcc gtcgacgatg aaatcattca ccgaacccag ttcgcgtttc cacataatct
     7621 gaccactgcg cagatcaagc gccgtcaggt taccattata ggccagcgcg aaaacaacgc
     7681 cgttaacaac gacgggagtc gtgtcaacat cgctcagacg gtcaatttcg gtagaaccgg
     7741 tcgcctggga aatacgctgc tgccaaatca tctggccctg ttccatcagc actgcgctga
     7801 cgcgaccatt atcgcccccc acgacggccg caccaaaagc cgttgccggc gcagactcgc
     7861 cacgcaaaga gagcgaaggc atatcgaggt taactgtcca tttgacagcg ccgtcagctt
     7921 cgttcagcgc ttgtaactga ccgttactgg tgtggattaa caccagaccg tcgctgacca
     7981 ccgggcgcga aagtgcttca cccgcgactt tagtttgcca tgccacagta ccatcgctgg
     8041 tattcagcgc gtaaacctgc gccttttcgc tgccaatgta gacatgccca ccagacacgg
     8101 tcacaccgcc agaaagtaat gcaggctctt tagagaacca gccatctttc tcggccaggc
     8161 tgacagacca gatttctttg ccatcatccg cattcagcgc ttttaccaaa ccagcgcggt
     8221 ccgctgcata gacaacgttg tccgccagtg ccggatgaag attggaatag aagttgccaa
     8281 tgccgctacc aacggaagta ctccacgccg tggtcggcgt aaactggttt tcaacggttg
     8341 gcaatgggga catctttacc acatcttctt cgctgttaaa cagcgaacag ccgcttaaaa
     8401 gggtaacgga aagcagtcct ggcagcagta atttacgcaa ttgcatcggg tccctctcag
     8461 atggacaaat tattaatttt catctgcatc atttcgctca gtgccggagt aacatcgctt
     8521 ttcacgcctg cttcccatgc actacgcgca ccttgcttat cacctttgct cagcaatgct
     8581 tcaccacgca ggtcggcaac aatggcagcc cacccttcac ctttgatggt atcaagggtt
     8641 ttcagcgcgg catcagcctg cttgagctgt acctgaacgc gagcaagacg cagatttatc
     8701 acggctttga gattttcatc gctcgtgtct gccagcccct gttgtaactg ggcggcagct
     8761 ttctctagtt catttttgtc aacaaattgc tgcgcaagtt ccaaagaagc cagcgcacca
     8821 taagtatttt tattttcagc agcaaatttt tccgccgccg ggatgctatc cggtttgcct
     8881 tcgctcactg cggtaaccgc attttgatag gcaagagaag cggagcgtgc agaatcaacc
     8941 tgatggctgt tccagtagcg ccagccaatc agtgcgccaa cgcccaaaat caccccaaca
     9001 gccagtgctt tgccattttc agcaaaaaag cgtttaaccg cttctacctg gtcgttttcg
     9061 ttctcgtaaa tttccacgct gtccttctcc ttccttaacc cagtaacgtg cgcaaatgcg
     9121 cggctacgct atcctgcgca actgccgttt gctcaccaga gcgcaaatcc ttcactactg
     9181 ctgtgccgtt agtcacttca gactcaccca gcaccacagc aacgcgggca ccccatttat
     9241 cagcacgggc aaactgtttc ttaaagttgc cgccgccgtg gttggtcatc aatttcacgc
     9301 cctgtaattc atcacgcaga cgctcagcta atgccatagc cgcagattgt gtatcagcac
     9361 ctgaagccac caggtatata tcgacaatag gatcggcttt aaattccgga ttaacggcct
     9421 gtactaacaa tacaagacgt tcgaggccca tcgcaaaacc gacagccggt gttgcacgac
     9481 cgcccagttg ttccacaaga ccgtcataac gaccgcctgc acacacggtg ccctgggagc
     9541 cgagactgtt agtcacccac tcgaaaacgg tacggttata gtaatccaga ccacgcacca
     9601 gacgctggtt tacggtgtaa gcgatccccg cgctctcaag cagtttgcac agaccggcaa
     9661 aatgctcacg agattcctcg tccagataat cacctaatgc cggagcgtcg ttgagaagcg
     9721 cctgcacttc cggatttttt gaatccagca cgcgcagcgg gttagtgtac atgcggcgtt
     9781 tgcagtcttc gtccagcttt tctttatgct gctcaaggaa tgccaccagc gcatcgcggt
     9841 aattggcgcg tgcttccagc gaaccgatag aattcagctc aagagttacg tgttcggaaa
     9901 tacccagcgc gcgccaccag cgggcggtga gcataatcag ttcagcgtcg atatccggac
     9961 cttgcagacc gaaaacttcg cagcccaact gatggaactg acgataacgc cctttctgcg
    10021 gacgctcgtg acggaacatc ggcccgatat accacagacg ctgttcctga ttgtacagaa
    10081 gaccatgctc gatgccggcg cgtacacagc ccgccgtccc ttcagggcgc agagtcaggc
    10141 tgtcgccatt gagatcctca aaggtgtaca tctctttttc aaccacgtcg gtgacttcac
    10201 caatcgcacg tttgaatagc ggggtctgct ctacaatcgg caagcggatt tcactgtaac
    10261 cgtagctgcc gagcacgttt ttcagtgtgc cttcaatgcg ctgccagatg gccgtttcgc
    10321 caggcaggta atcgttcatg ccgcgaatgg cttgaatgtt ttttgccacg tttattctct
    10381 ttctgaatat aaaaatgaac cctcaacgct tccctcaatg tttggggagc catgcgggtt
    10441 caatcataca cgggaagcga ggcgcttccc atcacgttat tatttttcaa cctgctgaac
    10501 gtcaattcga cgcgcttcat ccagctgact ggctttcgca cgaatgcgtg cttccagctg
    10561 gtcgatcata tcgttgttgt ccagacggtc tttgcgcacg ccatcttcat agaggccgct
    10621 tttcttgttg ccgccggtga cgccgagtgt agaaaccagc gcctcacctg ggccattcac
    10681 cacgcagccg ataatagaaa cgtccatcgg agtgatgata tcttccaggc gttgctccag
    10741 cgcgttaacc gtaccgataa catcaaattc ctgacgcgaa caggtcgggc aggcgatgaa
    10801 gttgatccct cgcgaacgga tacgcagcga tttcaaaata tcgaaaccga ctttgatctc
    10861 ttcgaccgga tcggccgcca gcgatacgcg cagcgtgtcg ccgatgcctt cagacagcag
    10921 cagacctaaa ccaatggcgg attttactgc cccgctgcgt gcaccaccgg cttcggtgat
    10981 ccccagatgc agcggctgat cgatctgttt tgccagcaaa cgataagact caacagcgag
    11041 gaagacgtca gacgctttca cgctgacttt gaactgatcg aagttcaggc gatcgagatg
    11101 atcaacatga cgcatggcgg attccagcaa cgcctgcggc gtcggttcgc catacttttc
    11161 ttgcagatct ttttccagcg atccggcgtt aacgccaata cggatcggaa tgtttttatc
    11221 gcgcgcacag tcaaccacca tgcgaatacg ctcttcatta ccgatattgc cagggttaat
    11281 acgcagacaa tcgacgccgt attccgctac tttcagcgca atgcgatagt cgaagtggat
    11341 gtcagccacc agcggcacgt taacccgctg tttgatgagt ttgaacgctt ctgccgcgtc
    11401 catcgtcggt acggagacac ggacgatatc agcgccaacg cgttccagcg ccttgatttg
    11461 attgaccgtt gcttcgacat ctgtcgtacg cgtattggtc atggactgta cggcgatggg
    11521 agcaccatcg ccaatcggca cattcccaac gtaaatacgt gttgattttc tacgttgaat
    11581 tggagcctgg ttatgcatga aaaatctccc gcgttacccg tctgttactg cgccggtgat
    11641 tgttcggcat tgagggtcag acgcgcaacc tggttagttc tgataaaacg actcagatcg
    11701 acaggtttcc cttgatactg gatctgtact gcggctggcg caccaatttt cagtttgtac
    11761 ggtgcctggc cggttaagtt caaattaccg tctttacgct gcataccgct aaacaatttt
    11821 ttaccggtag catcagtgac ctccagccag caatcggcag taaagttcat caccagcgca
    11881 ttcggatcag ccaccggcgt ggtcacgcca gcctgatcgg ttggcaacgg cgcagcacca
    11941 tctggcgttg ttgctgccgt tggtgccggg gtcgcggcgg tatcaacatt tgcctgcgaa
    12001 ggcgaaacaa ccgcattctg ttgcggatca acagctggtg ctggcgcagt tacggcaggt
    12061 gtttgcgtgt tggttgcggt agtatccaca gacgctggcg gcgtggacgt tgtagccggg
    12121 tctgtagttg tcgacgtatt taacggaaca ctctgcccct gctcgctatt actgctcagt
    12181 tccgccgaag attgatcggc catagtggtg atctcttcct gctgagcttt gtggtcttgc
    12241 caccaccagg caccgctcag gccgataacc acaaacaaca ccagccaggt gaaggtcatc
    12301 agccagccgt cacgtttttt gcggcgttta ccgagggaaa aactctgcat cggcgcaact
    12361 ttcgcagccc gtagtggagc ctgcttttcc agccctagca gcagttcttc ttctggaata
    12421 tgtaccagac gcgcataaga gcggatatat ccgcgcagga atgttgaagc aagatcggcg
    12481 ggtgccttat cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca
    12541 acggcctgct gactaagtcc tagttgttcg cgagcattac gcaggcgagc gccggtagta
    12601 agtgcttcat tttggtcgtg cgtggcttca gtattcattc gctgcaggta cgtttaaatg
    12661 agaattgaga tgccggtgaa ccatcatgct cacccacacc gcgaaacatc cgttaagtta
    12721 accgttatca tacagtataa gaccgtctgt gtgctcgtga caaagcccac attcactaag
    12781 gctaacttac tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg caccgttaat
    12841 attgaccaga caattgcaac ttaatacttc attcataaaa gattacgcca cggtacataa
    12901 agtaaccgtg gcgtaatggc tatcagaccg ctttaatgtc gatggcttca ccctgcatcc
    12961 gtttacgcag ggtacgtttc gtacggtcga taacatcgcc cgccaactga ccacaggcag
    13021 catcgatatc atcaccacga gttttacgca caatagtggt gaaaccgtag ctcatcagca
    13081 cttttgagaa acggtcgata cggctgttcg aactgcgtcc atacggcgcg cccgggaacg
    13141 ggttccacgg gatcaggttg atcttacacg gcgtatcttt cagcagctcc gccagttggt
    13201 gcgcgtgttc agtgccgtcg ttaacgtggt caagcatcac gtattcaata gtgactcggc
    13261 cctgattggc gttggatttc tccagataac ggcgcaccgc agcaaggaac gtttcgatat
    13321 tgtacttttt gttgatcggc acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg
    13381 aaattgccag tgcaacgtcg atcatatcgc ccagtttatc cagcgccgga actacaccgg
    13441 aagtagaaag cgtgacgcga cgtttagaca ggccaaaacc gaaatcatcc agcatgattt
    13501 ccatcgccgg aacgacgttg ttcaggttga gcagcggctc gcccatgccc atcatcacta
    13561 cgttagtgat tgggcgctga ccggtgactt ttgctgcgcc gacgattttc gccgcacgcc
    13621 acacctggcc gataatttcc gacacccgca ggttgcgatt aaagccctgc tgggcggtgg
    13681 aacagaattt acactccagc gcacacccca cctgcgaaga gacgcagagc gtggcacggt
    13741 cgtcttccgg gatatacacc gtttcgacgc gctgatcgcc aacggcgatc gcccatttaa
    13801 tggtgccgtc ggatgaacgc tgttcttcaa ccacttccgg tgcgcggatt tccgccacct
    13861 ctttcagttt gccgcgcaac actttgttga tgtcggtcat ctcatcaaag ttgtcgcagc
    13921 aatagtgata catccacttc atcacctgat cggcgcggaa gggtttttca cctaaatctt
    13981 taaaaaactc ccgcatctgc tgacggttga gatccagcag gttgattttt ccatctttcg
    14041 tggtgacgtt ttcaggtgtg actaattgtt cagacatatg ctattccggc ctcgttatta
    14101 cacgttatgg cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg
    14161 ggggcgctgc attgtacaaa ttctggcgca cggatgccac gtttgcacgc ggcatttacg
    14221 aaattattaa cgggtgcgcg ggcacacttc gccttcgcca aagaaataag cgatttcgcg
    14281 agcggcagat tcgacggaat cagaaccgtg ggtaccgttt tcggtcaggc tgtcagcgta
    14341 atcagcgcgc agagtaccag ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg
    14401 gtgacgctga acggcgtttt caccttccag cacggaaacc acgatcgggc cagaggtcat
    14461 gaattcaacc agaccatcaa agaacggttt tccatcgtgt tcagcataaa agccacgtgc
    14521 ctgttcaacg gtcaggtgca gcattttggt gccgacaatt ttgaaccctg cagcttcaaa
    14581 gcgcgcaaag atattaccaa tgacgttttt tgctaccgcg ttcggtttga tgatggaaaa
    14641 agtacgttca atagccattt ttacctctgt aaattgttct gttgttgtct gtaccagcgt
    14701 acgagatggc gcggattata atgagcaaca gggccgttga ctattgatga aggtaacatt
    14761 tttttaaaat aaaatgaggt ttagcaacaa tcagggacaa aacagactat tgcatgacaa
    14821 atttcactgt cgcgatttgc cccacgtcat ccatcaccag caattgataa tcacctttat
    14881 ccgtcaaatg cagggtcacg ttgcgcccgc gttcagttaa cggttcgcca ttcagaaacc
    14941 accagcgttc acctgcccct ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg
    15001 gtaaacgttt aataatcgcg ccatcgcgaa cgcctgtcag ctgcagcggg agctgggcat
    15061 agtgaccgta tggcgggcaa cttgtcgagg ccggtggtaa gcgcacagcg cgacgttctg
    15121 atgcgggcag ccagggttcc agcggtagcg gccagacatt tatcatttct tgtcgcgctt
    15181 gcgggcaatc agcggcaaca cgtttgccat tttcatccag ccagatgggg aaacgaatgc
    15241 cattaatgcc ttcctgctcc ggcagtaata gagttggcgg ctgactcccg tccagcagcc
    15301 aggttgccag gcggcggcga cagttaccgt caccttccgg caaagactgt ccgcccggcc
    15361 agcagataac gccacgagtg actgagttcg ggcgcgggtc ttccggcaga ttcgcactgc
    15421 gcgacagtaa gatattattg acctgattca acaatggtac ggcactggca aagccaaact
    15481 gaccaacaac gggcgtgccg tccggtctgc cagtccagat cccaatgaca tagcgagcgt
    15541 taaccccaat cgcccaggcg tcacgatagc catagctggt gcccgttttc catgccagtg
    15601 gggcgacgcg cggcaaggca ctatccggca agggttgcgc ttcatcagcc ataatccggc
    15661 gaatgatcca cgccgccccc gacgacatta aaggccattc aagcagcgga tcgtcaggct
    15721 gtaagcgcaa tttgcctgcc ttgccgtggc gagcaaacgc ggtatacgct gccgccatat
    15781 cttccagttt tgcaccagcg ccgccgagaa tgagtgaaag attcggcgca gcaccgttgg
    15841 gcaaatataa cggcaatcca acattgcgta actttgccgc aaaccgtttc ggtccatagg
    15901 cttccagcac ctgcacagca ggtaagttca gcgagcgcac cagcgcctcg ctcatgctga
    15961 tcgggccatg aaaaccgcta tcaaagttac ctggtcgata atcaccggtg cgccggggga
    16021 cgtcttgcag cagtgatgcc gggtggatca agccttcatc cagcgccaga ccataaacaa
    16081 acggtttgag caccgatcct ggcgatcgga tcgcattgac catatcaaca tgaccaaagc
    16141 gtgaatcatc gttgagatca accgatccca cccagccgcg aacacgcata tcggtatgat
    16201 caaccacgat catcgccagt gagctgcgcg gtggcaaccg ccctttccag ttttgcgcca
    16261 gttcttccag acgtcgttga agaccggcat ccagcgtagt aacgattttg tcgcttttgc
    16321 ttttaccgag catcatgcgc gaaaacagcg gtgccagttg cggcatttgt cggggtgcca
    16381 gccagatggg ttcttccctt gactctttta cctgctcacg ggaccacaca ccttgcacgg
    16441 ccatccgttc gagcacttta ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc
    16501 gcagtcggct gggcgcttgc ggtaacaccg ccagcattgc cgcctcggaa tagcttaaat
    16561 tcgcaggcga ttttccgaga taagcccaac ttgccgcacc gatcccctgc aacgtaccgc
    16621 caaacggagc gcggttaaga tacaagatca gaatttcacg cttagacaaa tgccactcca
    16681 gttgcaacgc gcgccagagc tggagaattt tgccaccaaa tgttttgggg tgaggatcaa
    16741 gcagacgagc aacctgcata gtgagcgtgc tgccaccaga aataacccgt cccgaagtga
    16801 gatcttgcca tgctgcgcgc gccaccgaga atggattcac ccccggatgc ttccagaacc
    16861 agcgatcttc ataattgatc agcgcttcaa ggtaacgtgg agacacatct tcgattgtta
    16921 ccggataacg ccagatgcca tcagcatcgg cgaagcgcca gagcggcgta ccatcctgcg
    16981 ccacgaccac tcgtgcggga ttgacttcat gcagcggtag aggccagagt ttatctgctc
    17041 cccacgctgc aagaaaaagg agaaatggca cagctgccag cgttatccag cagccgcgtt
    17101 tggttaacaa gcgaagcatt tacggtcgga caatcagcag atcttcagcc gcgccggtcg
    17161 cccgccattg gggaacatac attgattcca ccatcggttg cggtacctga tacgttcccg
    17221 gcgtcaccgc ccgcgccaga tacaccagcg ttaccggttg gtattcatca acggcaaccg
    17281 ccgccacaaa gcgatcgtca cggaactcaa tgtgcttaat gctcgcctgc tgcatctggt
    17341 tcagtaagtt ttgcacttcg tcaccacttt gctccaggct ggcgctaccg ttcgccagat
    17401 tctgattttc cagttccaga cccgcaggca gcagatccac gactaacgca tccggcacgc
    17461 tgttactggc ttttacctgc aaccagacca gcaccagatc gccgctacgt aacgagtcca
    17521 gcgatttgct cttaccatca gtaccaagaa tatgacgctc gatttgcagc acattgttcg
    17581 ccggtaaagg tgcggattgc ggataaccgc tggcatccat acgcaaccat aacggctgat
    17641 cgccactgtt tctcacctgc aaggtggcaa gttgatcgct attcagattg ctgtttagcg
    17701 ttttctcacc tgtcagcggc tcagctgaga aagaggtttg cgcctgccat ttacccggta
    17761 aatcctgaat cgtacgggca gccaggaaca acgcgttact ttcctgcgtc gatagccagc
    17821 gttcaccaaa cgcctgctgc gaaagtgtgt tcagcaaagt gtactgctca tcgggtagca
    17881 gtttattttc ttccagcaag gagagcatta acgcgttgtc gcgcagtgga ctaccgtaat
    17941 cacccagcca tatccgctca tcactatttc tcggcgtttt cagcgccagc acaatcgctt
    18001 cttcaccacg cgttgcatca cccatggttt tcagcgcaat gccaagttgc agcagcggta
    18061 aaccagaagc ggcatctgca cgatgctccc agatttcacg cagcgcaccc agtggtgctt
    18121 tttgctgacg ggccagcacc agcgcagcgt aagactgaac ggcgaattta ctggctttga
    18181 gattatccgc gtacgggatc gacatcatgc ccggatcttg taaatagcgc agcagacgct
    18241 cattaccccg gttaatggcg tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca
    18301 ggaaatccat cacgtaagcc gtcagccagt actcttcgtc accgttttta tcccacagcg
    18361 caaagccgcc gttatcacgt tgcatttgca gcaaacggga aatgccgata tcgaccgatg
    18421 cacggcgttt ctcatcactg tcgcctttga tgcccaacgc ctgcagttgg gctgcgttgg
    18481 tataaagtga cgggaacagg ccgctggcgg tttgctcaag acagccgtac ggatacgctt
    18541 ttaactcttt gatataacgt gcaatgttca gcggtggttt gccgctcaac aacaattgcc
    18601 cttccagcgt aacaggcgag aagttttgca atccatccgc cggaatcgcc catgtctcac
    18661 caggctgtaa cgccgtaccg taattaaccg tttgtgccgg gaacgccgga cggacgccga
    18721 ttttccactg cttatgctga tcggcaacgg tttcacccgg taacgctaac ccgctaatgg
    18781 tggcctgaat ttctccatcg ccataacccg gcaatgctcg caccgggata aacagcgtag
    18841 tacgcacacc tggcgctaat tcaacggctg cgggtgaatc gctgaccagt tcaagcaaac
    18901 cactggcggt cagggcaacg ttcagttttt gcggtttatc ggtaagatta gtgatatcca
    18961 gcgtcagacg cgaggtatcg ccactcgcca taaagcgcgg catgttcagt tcagcaatca
    19021 ccggtgcggc aactatcact ttgctttcgt tgctaccgaa atcatctgcc gtccaggctt
    19081 gcgccatgac gcgcaattca ccgttaaaat cgccaatcgg cagtgtaacc gagccttcgc
    19141 cctgttcgtt gagcgttacc ggcagcgcct gctgcgcgac aatattgacg tgattgacca
    19201 gcggtttacc accacgtttc agctcatcac catcgccacc gaaacgcaga gctgccagac
    19261 gcccctgacc ttcaataacc tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct
    19321 gaccaaagaa cgcctgccac ggatctggcg tgacgtagtc agtaatattc agcacaccac
    19381 tatcaacggc agacaccagc acattcacct gtttaggctt ctcgccattt ttagtgctgg
    19441 ctttaatttt cacggttaat ggttgattgg gacgcatttt tgctggtgtt tccagcgcca
    19501 gatcgaggcg acggttttca tcgccaagcg gcagatgcaa cacaccaacc gcgcgttttg
    19561 gcgtcgcgga gcgagattta tcgctaggac gtaccaccag cgtacttaaa tacagatcat
    19621 gacgattcca ggttttatcg accggaatcg tcagatccag cccttgagcc ggaacatcaa
    19681 tctcttgcca ccacagcggc ccttcactgg actcgaccat cgcataacct ttacccgccg
    19741 ttggcgcggc gatatgcaac ttaatggtgt cgccagggcg ataactggct ttatccagtt
    19801 tcagcgtgac acggtcgggt cgcactgcgc cgctaccgtc gctgttgtcc tgccaactat
    19861 agccagccca gaaacgaaca ctactgaccg cttcattcgg cgctttgact tccagacgat
    19921 aagcgcccca ctctaccgga aaactgacct tgccggtttc gtccgctttc agatccagag
    19981 tttgttcatt ttcgatcaga tctttttgat caaactgtga ctgccagcct tcatcttctg
    20041 accagttcca gtagtaatcg cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg
    20101 cttttttcac gccttgtgca tcgctataaa cgatgtcaaa agcggcgtta ctgccttcat
    20161 caacaatggg ctgtttcacc gtgctgtcag tgcgataatc gtaaaccgat ttcaaggcga
    20221 actgcggacg gagccccggc aatgcattgg caggccagat agcctgctca gcgcggcgcg
    20281 tcaccgggcg accgcccgat tccagcaaac tgccctggaa aataacctgc aatggggaat
    20341 gcgtttcctt ccactggctt tctgtagaaa cttcgccgcg ccctttatca tccagcgtca
    20401 actgaacttc atccagcgtg cgggaaaggt tttcggcagc tatatcgccg aattcaaaac
    20461 caggtaaggc tgacacagcc tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat
    20521 taccattagc aggcgcaccg tacaggtagt atcccaccac ggagaatttc acttcatcat
    20581 tcggcgtaag cggggttttc tcaccggtca gattcagcgc catacgctct ggcataaaat
    20641 cttcgacgtg gaaatcccac atccggtact ggttatcgcc cgtgttggcg cgaatatgcc
    20701 acataccggt tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg ccattctccg
    20761 gctgactaac gacgctcctg agtacctgcc cgtcaggttt aatcacgtct aacttgatgg
    20821 gttgatcggg caatgcttta ccgtctgcat cgcgcagtaa accattgagg attaccgttt
    20881 cacctgggcg ataaagatcg cgtgggccaa acatgaaaaa ctgtttgcta tagcctggcg
    20941 cgccagcaat gttaaattct gctaagtcca gcgccggaag ttttaaatcg agtagcgttg
    21001 tctgaccgtc tttacgtgcc agcagtaatg ccgcattttt atcattttcc agctgcacat
    21061 gcccctgagc gtcactggtt gcctgagtca gagtctgccc tttctcattt aataaagaga
    21121 cttcaattcc ttgctgggcc gcgccgtttt ccagactttg ggtaaagata tccagacgat
    21181 tgtgataacg gtgagctgaa acgccgatat cacttaacgt aaacagcgtc gcgggattac
    21241 tgtaatcgta acgtccagcc tgattcatca cagccagata cacgcccgcc tgctgaagcg
    21301 gtttgatatc gcccagcggc agcaataatt tttcacgggt gttacgcgca ggattgagat
    21361 caaaccgtcc ggtgtagacc agatccgcca tctgcagcag tttgtctgac tgccagttcg
    21421 ccagcgaatt gcggtattcc cattggctaa tgaatgctgg cagagattct ggcttaacgc
    21481 ggaagaagtt aacatcgaca ttattaacgt tgagcgccat taccggcagc ccttcaacga
    21541 ctttgccagg cagcagcgaa ccacggctgg caaaaccgac gctgggttgg atgtcgcggg
    21601 tagttatagt tttttcgtaa tctttactga aggttgcgtt gttgagtgct ttgacctcct
    21661 tgccaatagt aacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgt agctctttaa
    21721 gattatctga cagctcccag gcaccatcca ctttgccgct ttttttatcg acgacatgaa
    21781 taacgcgtga gaaatcctga tccgggtcga gagggatgga gaacgtcagc accagcgtgg
    21841 ctgcaccatc aagttggact tcagagagat ccagcaatgt cagcacctta ccggcactct
    21901 gttgggccag tttttgtctc tccggcacgg agagttttgc tgaactcgcg ttttctgaag
    21961 aggccgcttt agtaacttca gaaggcgcat cttttttcac cgctgttggc gcgttatcgt
    22021 tgttgtcgca ccctgccagc gccagcatta gcatgcaggc ggctacgcgt aactttttca
    22081 tttttcatcc ctgccacaat ggcccgttag caacgtcgaa taattattat gcgtgagaat
    22141 ttacgttatg taattcagta
//
LOCUS       DAJVOY010000056        22106 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVOY010000056 DAJVOY010000000
VERSION     DAJVOY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.056"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..269)
                     /gene="wcaD"
                     /locus_tag="OQL90_001871"
     CDS             complement(<1..269)
                     /gene="wcaD"
                     /locus_tag="OQL90_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1368671.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFV"
     gene            complement(244..1461)
                     /gene="wcaC"
                     /locus_tag="OQL90_001872"
     CDS             complement(244..1461)
                     /gene="wcaC"
                     /locus_tag="OQL90_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1368672.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1458..1930)
                     /gene="wcaB"
                     /locus_tag="OQL90_001873"
                     /pseudo
     CDS             complement(1458..1930)
                     /gene="wcaB"
                     /locus_tag="OQL90_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
     gene            complement(1933..2772)
                     /gene="wcaA"
                     /locus_tag="OQL90_001874"
     CDS             complement(1933..2772)
                     /gene="wcaA"
                     /locus_tag="OQL90_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS1368673.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGH"
     gene            complement(2950..5112)
                     /gene="wzc"
                     /locus_tag="OQL90_001875"
                     /pseudo
     CDS             complement(2950..5112)
                     /gene="wzc"
                     /locus_tag="OQL90_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
     gene            complement(5115..5558)
                     /gene="wzb"
                     /locus_tag="OQL90_001876"
     CDS             complement(5115..5558)
                     /gene="wzb"
                     /locus_tag="OQL90_001876"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1368674.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSWETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5564..6578)
                     /locus_tag="OQL90_001877"
                     /pseudo
     CDS             complement(5564..6578)
                     /locus_tag="OQL90_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
     gene            6887..7036
                     /locus_tag="OQL90_001878"
     CDS             6887..7036
                     /locus_tag="OQL90_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368675.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            7237..8820
                     /locus_tag="OQL90_001879"
     CDS             7237..8820
                     /locus_tag="OQL90_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1368676.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(9094..10947)
                     /gene="asmA"
                     /locus_tag="OQL90_001880"
     CDS             complement(9094..10947)
                     /gene="asmA"
                     /locus_tag="OQL90_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1368677.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(10969..11550)
                     /gene="dcd"
                     /locus_tag="OQL90_001881"
     CDS             complement(10969..11550)
                     /gene="dcd"
                     /locus_tag="OQL90_001881"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1368678.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(11642..12283)
                     /gene="udk"
                     /locus_tag="OQL90_001882"
     CDS             complement(11642..12283)
                     /gene="udk"
                     /locus_tag="OQL90_001882"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1368679.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            12601..15898
                     /locus_tag="OQL90_001883"
                     /pseudo
     CDS             12601..15898
                     /locus_tag="OQL90_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(15937..16794)
                     /gene="alkA"
                     /locus_tag="OQL90_001884"
     CDS             complement(15937..16794)
                     /gene="alkA"
                     /locus_tag="OQL90_001884"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS1368680.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            16928..18280
                     /gene="yegD"
                     /locus_tag="OQL90_001885"
     CDS             16928..18280
                     /gene="yegD"
                     /locus_tag="OQL90_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1368681.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(18292..20214)
                     /gene="yegI"
                     /locus_tag="OQL90_001886"
     CDS             complement(18292..20214)
                     /gene="yegI"
                     /locus_tag="OQL90_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCS1368682.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(20211..20972)
                     /gene="pphC"
                     /locus_tag="OQL90_001887"
     CDS             complement(20211..20972)
                     /gene="pphC"
                     /locus_tag="OQL90_001887"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCS1368683.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(20969..21628)
                     /locus_tag="OQL90_001888"
     CDS             complement(20969..21628)
                     /locus_tag="OQL90_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1368684.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(21849..21908)
                     /gene="ibsA"
                     /locus_tag="OQL90_001889"
     CDS             complement(21849..21908)
                     /gene="ibsA"
                     /locus_tag="OQL90_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCS1368685.1"
                     /translation="MMKHVIILVILLVISFQAY"
BASE COUNT         5196 a         5845 c         5653 g         5412 t
ORIGIN      
        1 tagacaaaca gcatggttga agtgacgtat ttactggcat ccagcgactg accaaacagg
       61 tagttaaacg ccgtgagtcc cgcaccaatc cctaaggcaa tcatcaattt tttaacgctg
      121 atgcgttcta aaaacagcag taacaagaca ggtaaaaaag tgacgatagt gatggggaaa
      181 ctttcaccaa gctgggcgat tttgacgtta accagcaggt agatcagcgg cagcagcagg
      241 tagctacaga ttctgataga agttgacata ctcctccagc atctgttgtc cactgtaggc
      301 ggcgcggctg cgctggctga acccggccaa cgtggtacca aatatcgcct gcgcgatttc
      361 cggtttgctt aattgcacta gttgcagcac ctcttcttcg ctgacggttt taccgccgga
      421 tttttgcaac acttcccgcg ccgcgtcgct atgggtggca atcaccggca cgccaatcga
      481 tagcgcctca cacaaaatca gcgggtagtt atcgacgcga gaactgaaca ccagcgcatc
      541 catctgattg agcgcgctca tcaacttgcg cttgtcagtt tcaaagccgt gattaaccac
      601 gttgccagcg gtgaacggcg agaacttacc aaaggtatgc agttcgattt tgtcgcccag
      661 cgccatcatc tcacgcacca gttgctggtt agtcttgccg tcgtaacgca agtcatgcgc
      721 caccaccgcg attttcggct tgccctgggt ttcgcgcacc ggaggcaagt ccgccagaat
      781 cgcttcggtt gccatatcaa taccattatt gataatccgg caacgccctg gaccgtacag
      841 gctattgaaa gcgtcagcca catgctggct gggggaaata aactgacagc ccagcgccag
      901 catctcacgg aataactggc gtttgcccgc caccagctgg tgtgcgcgat caatcttcac
      961 cggcggatag ttattcaagg tcgggcattt ctggcagccc gttttccagc cttcgcaacc
     1021 gtcggtaaag gcgcagcgtc cggtaacact ccagtggtcg tgcagcgtcc agaccagagt
     1081 gacgtccggt ttatggtttt tcactttttc gcaaaagcgc accacgctct taagatttag
     1141 ccagtagctg tgcagcacat gaaaatgcag gaccaccgga cccggtgtgc gagtaatggt
     1201 gcgatataac tcattgaaat tgccaaacag atcgcgatta aacagacgaa acagggcaat
     1261 attcgccatc gcggtcatcc gcggcgtatg tttgatgacc tgcggatagt tctgatggct
     1321 gacgctctct ttgccgcctt tgccgtaacc gtagacaaaa tgtgacgcca gcccctgttg
     1381 cagcgcacgc tggtggagat ctaacgccac acctgctgcc ccgccttccg ccagtcgcac
     1441 attaaattgc agaatattca tttaattacc ttcactcgcg ctttttctcc taccaccagc
     1501 gcgttgtccg ggacagagtc gagcaccacg ctgcccgcac ccacggtgac gttgttacca
     1561 agcgtgatat caccaagaat aatgacgttg gcaccgagtt cgacgccgtt gccaatgtgt
     1621 ggacatgcca tgttatcggc accacgattg ccgatagtga cgccgtggcg aatggtgaaa
     1681 tcatcccccg ctaccacgtt tttattgatc actacggcgt aaccgtgatg gatagtaaag
     1741 cggcggcctg gatttcataa ccgaaaaagc attcggtgat aatgcgatac agcaccagca
     1801 gcggggccgc ccacagattg ttgaggacgt tctttttgcg ccacaccgaa caaaaatgag
     1861 caacacgata ggcaagaacc atgcagcacg ggcgtaaact ccagctgttg gcacgcagat
     1921 cttccagcat ctttagtgcc cccgaatacc atcagccaga cgtttgccgt tacgcaccga
     1981 cagcagcgtc agcaaagtgc gccaggtcat acgtttattg cggatctggt agagggtaaa
     2041 cagctgatat tttttgcttg cacggtcaaa tttatctttg tgctttcggt aaaagtggaa
     2101 gtacccggag aattttttcg gtgacgaggt gatctgcatt tcaccgtgat tgatatgcag
     2161 gatctgcgtc gcttcttcca ctttccacgg ttcgccgtac tccaccacca tccgcaggaa
     2221 gatgtcgtaa tcctgtgctg ctttcagttc ggtatcgaac aaacactctt tgaaacgcca
     2281 tgcccaagta aagacctgat tgccaatgat attgcgttta tagaacaggc ggcgtgaata
     2341 cggcgatttg ggatatagcg gcaaacttgc tggttgggaa tagacttcgc cctggcaaac
     2401 gtagtcgtta gcgtacaaaa aggcgtgagt gaccagttgc tgtttatggg cgaggaagac
     2461 gctcagacgg ttgggtgtcc attcgtcatc gtcatcaatc ccggtgatgt attccccttg
     2521 cgccagcata atcgcctggt tacgtaccgc gcacgccccg ctgttaatat cgttgtggat
     2581 gtaagtgata cgcggatcgt tgagggcggt gacgtactgt tgcagttgtt cccaggaggt
     2641 ggagcaatca tccacgatga tcatctccca gttgctgtag tcctggcgca ggaccgattt
     2701 tatcgcccga atcgccagtt gttggcggtt ccaggtcggc atatagattg agatcagcgg
     2761 attgtctttc ataggttgtt ctcccggagt gtcgagatgt tctgccggtt tataagcttg
     2821 tttacgggtt ctgttgccgg atgcggcgtg agcaccttat ccggcctacg gagctggttt
     2881 gcttgccgga tgcggcgtga aggtaggtcg gataagatgc gtcagcatcg catccgacag
     2941 cggatgtcgt tatttcgcat ccgacttata ttcgtattcg taatagccat aatcctgata
     3001 cgcgctggcg cggcggaaga tggagttcag aatcacccct ttcaccggaa taccgttttg
     3061 ctcaaagcgg ctcagactgg tttccacttc tttcaaggtg ttgaccgcat aacgcgccac
     3121 cattaacgtg gttccgacat gacgaccaac aattgccgca tcggtcactg ccagaatcgg
     3181 cggcgtatca atcaacacca ggtcatagtt tttactcgcc cagttcacca gttcggcaaa
     3241 gcgttcgctc atcaacagtt cagaaggatt tggcggcacc tgaccgcgcg ggatcaggtc
     3301 aaatttggca atagaggtcg gtttagcagc ggtagtaata tcgccctgac caatcagaat
     3361 ttccgacagg ccattaacgt tattagtgca cagcagctcg tgggtgtagc ctttgcgcat
     3421 atcgcagtcg atcaacaaca cccgtttatt ggtctggctg atcaccgccg ccaggttggc
     3481 gcagacaaag gttttaccga ttgacgggct aaccccggtc atcatcaaca cattgttctg
     3541 tgcctgcatc atcgcgaagt gcaaactggt acgcagactg cggatggctt caatcgccag
     3601 atcggttgga ttccccatcg ccagtagctg gctctgttta tagcgtttaa tccctttgat
     3661 ggttttgacg ctatcgcgcg ctttctgcca ttccgacagc gggatgctgg catagacgct
     3721 gataccgtgt tcttccagca cctgcgggct ttcgatgccg cgattaaaca acgagcgcag
     3781 cagcacaccc acgatagaaa gcatcaggcc aaggataatc gcgccgagga taatcagccc
     3841 tttcttcggt tttagcacgc caggctgggt gattgccggg tcaacaatgc gcacatcgcc
     3901 gacggtgctg gcttcggtga ttttcagctc ctgctcttta ttcagcagtt gcatatagac
     3961 ctgctgacca gactcgacat cgcgggtcag gcgcacaatc tcctgctggg ttttcggcat
     4021 cgccgttacg cgaccgttaa gtttggcttt ctcttcttcc agcgcctgac gtttctccag
     4081 cagcgtgcgg tacgccgggt gaactttggt gtacagcttg gagatttccg cctctttaaa
     4141 ggtcagttcg ttcaactgcg cgtcgatgtt caccatcgaa tcgagcaccg ctttcgcttc
     4201 cagcggcaga tcaacagaat ctttatcctg acggaaggca ttcagtttgt tttcggcaac
     4261 atcgaggcgg ctacgtactt ccggtaactg ttgcgcgagg aaagcgaggc ttttcgacgc
     4321 ttccgccgat ttgcgctcaa tattttgttc ctgatagtta cgggcgatgc tgttaagaat
     4381 gtcgcggatc tgttcgcgat cttcaccggt ataagtcagg ctcagtacgc ctgcgtcttt
     4441 gccgttctcc gttaccatca ggctgttttg cagttggttg atcatcccca acgtggagta
     4501 tttggtgacg gtaaactcac tgcccgggcg ggcgtgaatg gcttcaacca tcagcgtgac
     4561 gccttctttt ttcagcatct ggcccgcttg cccacgggcg ctaaagccgc catcactgct
     4621 cagggtgtag tttttattgt ccagcacatt aagcgtaaac acctagtccg ccatctcttt
     4681 cgggcggtta aaggtagtca ctttcaccgt ctcgttctgg cgtcccatca gtcgatccca
     4741 gcccgccccg aaaatcggga acgtgttttt gctcactgca atatcgaggt cgagatcgtc
     4801 caccgtttta ccaagcacca gacgcgaacg aatcaactgg atctcggcgt ccgatgcagg
     4861 cggtttgttg gctaatgccg aaccaatgtc ctgcactaac gaattgccgc tgttttgctc
     4921 gatttgtacc agtgcgtcgg cactataaat cggcgtggcg aagaaggtgt aaaccacggc
     4981 acagaggaca aatacagcgg tgatgccaat cacccaccag cgcgcttcaa tgacggtgcc
     5041 gaccaggcga ccaatatcga tttcatcact gcccgttacc ggagcggcat gttgttttac
     5101 tttttctgtc attcttatac ctgctctgcg ttcaatgcct gcgcccactg gcgggcagac
     5161 cgttcaagta atgtgtacac cgctgcaaac gtttcccagc ttttgcgata cggatcgggg
     5221 atttcacatt cgttatccca gtgaccaaac agcatcactt tgccgcgcat ctcaggtgcc
     5281 atctcgcata agcgttcgat atggcgcttt tccatggtca aaatcaggtc atagttgcga
     5341 cacaggcggc ggctgatttg tcgtgcacag tggccttcca gagacagttg atgttctgcg
     5401 gcgacgctga tagcggtagg atcagcgccc ttaccgacca gcgcgccgag tccagcggac
     5461 tccactttca gttccgggtg ataacgttgc agtaagcgtt ccgccgtcgg ggaacggcaa
     5521 atattgccga cacagacaac taatatgttg ttaaacatga cgattaccag ttatgaatgt
     5581 cgctggctgt atccgtcatg taacggacac cgctaatagt tggcaatctt gccgttacgc
     5641 ccgccctcgc ctttcagcgg acgaatgaca aagatgccgc tggcgttgga ggtggtcatg
     5701 tcgatacctt cagcattacc cagggcttca gtcagggctt cagtcagggt catgccgcta
     5761 aagtccattt tcagggtgct ctgtttcttc acttcaccca tcacaaatac tttcagatca
     5821 tcattacgcg gcacgtagag aatatcgccg ggataaagca gtcggttctg attgaggtcg
     5881 ccgttttgca tcagcacctg caaagaaatg cgttcttcac gaccattgtg tgttagcacc
     5941 acgttgcgcc agtcagcggt gtcggtcagg ccacctgcgg cgttgatggc gtcgagaatg
     6001 gtcagtggta cgttggtgat cgcctgttga ccggatttat tcacctgacc tgagatatag
     6061 gtcttttgtg agcggaaggc ggcgatatta acgtccacct gcgggtcagc gatgtacgtc
     6121 gctaagcgcc cggtaatatc actgcggatt tcagcgagcg ttttcccgac tacgtgaacc
     6181 ttgccgatat acgggtaaaa catagtgccg tcaggctgta cccagttgcc ggtgtcgctg
     6241 gagctgcggt actgaccagc tggcgtggtg agttccgggt gatcccagac ggtgacatta
     6301 agaacgtccc ccggcccgac gcgatactgg taattcgcga tctcgctttc cagcgtcata
     6361 ttggggcgcg ctacattcgg gcgtgggcgt aattggtcaa tcaggcgcgg ggtcagcgga
     6421 taaacattca ccattttgtc gagatcgaaa tcagcgtcct gctgtttgat gacgtctttg
     6481 cccatcgtcg acatattgct gcccggaagt actgtgcaac cgcttatcaa ggttactgac
     6541 accaataatg gcatcaattt cattttggat ttcatcattg tttatttatc actttggcag
     6601 agtaattatc ctgtgcacta ttaatagcaa tgtcgccatg cacatttacc ttgcagttaa
     6661 ttgaatacaa atttaactgg catcagtcct aaaaaaattg atttcatccg caggctattg
     6721 acagaataat tcagactggt ctttcaggca tccagacacg ctaccgcccc tggcttttta
     6781 gccaccaata cactgattta gcttaatttt ttaaaccctc tctgcataca atcgttgatg
     6841 agaaagggtt attgcggaaa ttaacttccg aatataaggt gacattatgg taattgaata
     6901 ttggctctcc aataatgcag gaggaagtgt tacagctaac ggaatagcag gcaaggtaac
     6961 aattcggcaa ttggctattt ttaagaatta tattaagtgt cattcaatat ggtttttagg
     7021 agtttcttta ggttgacaat atttaatatt gcgcctccgc atgcgatatt tcttaaataa
     7081 tgttttatta ttaccacttt taattcaggg ataatgtgag gttattaccc tcaaataata
     7141 tgagataaat agtgctgcaa cattgcattt ttgccccgat ttatccatga tcgaattgtg
     7201 acatttgtca tacaacgaat aggttttgta cttactatgg aatggattgc cgatccgtct
     7261 atctgggccg ggttaatcac gctgattgtg atcgaactgg tcctcggcat tgataacctg
     7321 gtctttattg ccatcctcgc cgaaaaacta ccgccgaagc agcgcgaccg cgcacgggtt
     7381 accgggctgt tgctggcgat gttaatgcgc ctgttactgc tggcgtcaat ctcctggctg
     7441 gtcaccctga cgcaaccgct gttcagcttc cgctcgttta cctttagcgc ccgcgactta
     7501 atcatgctgt ttggtggttt cttcctgttg ttcaaagcca cgatggagct gaacgaacgg
     7561 ctggaaggga aagacagcaa taatcccaca caacgcaaag gtgcgaagtt ctggggggtg
     7621 gtgacacaaa ttgtggtact ggacgccatc ttctcactcg actcagtgat taccgccgtc
     7681 gggatggtcg accatttact ggtcatgatg gccgccgtgg ttatcgctat cagcctgatg
     7741 ttgatggcca gcaagccgtt aacgcaattt gttaacagtc acccgaccat cgttattctc
     7801 tgcttaagct tcctgttgat gattggcttt agcctggtgg cagaaggttt cggcttcgtc
     7861 attccgaaag gctacctgta cgctgccatt ggtttctcgg taatgatcga ggcgctcaat
     7921 cagttggcta tctttaaccg gcgacgtttt ctttccgcta accagacgct acgccagcgg
     7981 actaccgaag cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga actggacgcc
     8041 gaaaccgcgt ccatgctggt ggatcatggt aaccagcaga tctttaatcc gcaggaacgg
     8101 cggatgattg agcgggtact taatcttaac cagcgtactg tcagcagcat tatgacgtcg
     8161 cgccacgata ttgagcatat cgatctcaac gcgccggaag acgagatccg ccagttgctg
     8221 gagcgaaatc agcatacgcg gctggtggtt accgacggtg atgacgcaga agatttgctc
     8281 ggtgttgttc acgttatcga cctgctacaa cagtcactgc gtggcgaacc gctcaacctg
     8341 cgggtgttga ttcgccagcc gctagtgttc ccggaaacct tgccgttgtt acctgccctg
     8401 gagcagttcc gtaatgcccg cacacacttt gcttttgtgg tggatgagtt tggctcggtg
     8461 gaagggattg tgacattaag tgacgtcact gaaaccattg ccggtaactt accgaacgaa
     8521 gtggaagaga tcgacgcccg ccatgatatt cagaagaatg cggacggttc ctggacggcg
     8581 aatggtcata tgccgctgga agatctggtg caatatgtgc cgctgccgct ggatgaaaaa
     8641 cgtgaatatc acaccattgc cgggctgttg atggaatatt tacagcgtat tccaaaacct
     8701 ggcgaagaag ttcaggtggg ggattatttg cttaaaacgt tgcaggtgga aagccatcgc
     8761 gtgcagaagg tgcagattat tccgctgcat aaggatggcg agttggagta cgaggtgtga
     8821 tggtatcggt cattttaccc aatagccgaa aggaataaga tgagggaacc gcgccagcgg
     8881 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagcccc cctggcacgt
     8941 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9001 cttacatatt cagaatataa tgatgccgcc acgaacgcct gatgcgacgc tttacgcgtc
     9061 ttatcaggcc tacaggacag gtcaccaggt aacctacatc ttctccagca acttcttcac
     9121 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcagac gacgtttcgc
     9181 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc agactgtaat tgagttgctg
     9241 ccagttgcca taaacccgca gcggtactgg cgtttcttta aggaaatcaa tcagtttgct
     9301 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgctccgc
     9361 cagattcaac gtaccagccc cggaaagcgc cagcacaggc gattgacctt gcatgtcgtt
     9421 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gacgcgttac
     9481 gttatcgaag ttttcagcgg ccttcacatc accaccatta cgttctaccg cttgctgaat
     9541 catctgctgg aagttcatcc cttccatgcg cgtgtcggtc atttcgacat gtgcctgtcc
     9601 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
     9661 tgacatcttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg tgccaatctc
     9721 aacgttttcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
     9781 gcccggcagt gaaacctgtc caccgttaag cttgccctgc agttgagtaa tttccagcaa
     9841 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
     9901 gttactggcc tgcaacaaaa tatcagccgt aaagccttgc agtccctgat atgccggttc
     9961 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10021 ggcaccgttt tcaccattcg ctgtttcatt aagcgggatg aggttgtcaa gattcagttg
    10081 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgccc
    10141 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10201 ctcctgccac tgggcctgga aactcccctg cccatgaata ccttgttttg gtaaatctgc
    10261 accctgcaac tgccagttaa tttgttcaat agccgccgtt aaatcatgcg gataatcaga
    10321 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    10381 ggagaactca aatgaaccac gatgttgggg atcttgttcc atttgcaggc gaatattgcg
    10441 gattgtcacc tgctcgtcat cttcatgctg gaacaccagc acgctgtccg ccaccttaag
    10501 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    10561 cggtgcaacc ggagcgtctt cactgcgcac cacttccgtc tgcggcgtca gttggatcac
    10621 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    10681 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg ctcgcgccct gggcggtgag
    10741 agacattcgc ccagagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    10801 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt tgcggaaatc
    10861 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaggagtat
    10921 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    10981 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11041 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11101 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11161 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    11221 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gcgtcaccgc
    11281 cagcgccagt tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    11341 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    11401 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    11461 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    11521 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcgc atcactccct
    11581 taacgccaat tacgtcaagg gcataccagg ttatcactga acggtacaca atttatcaag
    11641 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    11701 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    11761 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    11821 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    11881 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    11941 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12001 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcagcaggt
    12061 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12121 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12181 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    12241 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    12301 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    12361 tccgcctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    12421 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    12481 gcgcaaatta tccgccttta cgtcacttta tgagcaattc acatataaaa tgtaaaactt
    12541 ttgtactagc ataaacacag aaacgaatac tggcgacctg gtcttgcgga taaagcggta
    12601 atgagcaaac aatcacagca tgtattaatt gccctgcccc acccactgct tcacctggtc
    12661 agtttaggtt tagtctcgtt tatctttacc cttttctcgc ttgagctttc gcagtttggc
    12721 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    12781 gccgggcgca tgtggccggg aattgcgctg agctgctcgc tgggaaatat cgccgcatcc
    12841 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa
    12901 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat
    12961 ctggctgact ggctgcgtct ggcattcggc agcgccattg ttccgcctct gttagggggt
    13021 gttctggttg tcctgctgac gcccggagac gatcctctca gggcattttt gatatgggta
    13081 ctgtcagaat ccatcggcgc tctggtactg gtgccgctgg gattgttatt taagccacac
    13141 tatctgctgc gccatcgcaa cccacggtta ctttttgagt cactgctcac attagccatc
    13201 acgctgacgt taagctggct ttctatgttg tacctgccgt ggccttttac tttcattatt
    13261 gtgctgttga tgtggagcgc cgtgcgcctg ccacgaatgg aagccttttt gatcttcctt
    13321 accacggtga tgatggtgtc gctgatgatg gccgcggatc cctccctgct tgctacgccg
    13381 cgtacgtacc tgatgagcca tatgccgtgg ctactgtttt tgctgatcct gctgcccgcc
    13441 aacatcatga ccatggtgat gtatgccttt cgtgcggaac gcaaacacat ttccgaaagc
    13501 gaaacccgct ttcggaacgc gatggaatat tccgctatcg gtatggcgtt agtgggcacc
    13561 gagggacaat ggctgcaaac caacaaagcg ctctgccagt ttctcgggta cagtcaggaa
    13621 gagctgcgcg gactcacctt tcagcaactg acctggccgg aggatctcaa taaagatctc
    13681 caacaggttg aaaagctgat aagcggtgaa ataaacacct attcaatgga aaaacgctac
    13741 tacaaccgta atggcgatgt tgtctgggcg ttgcttgccg tctcactggt gcgccacacg
    13801 gatggcacgc cgctctattt tatcgctcag attgaagaca ttaacgagct aaaacgcacc
    13861 gaacaggtga atcagcaact gatggagcgc atcacgctgg ctaacgaagc gggcgggatt
    13921 ggcatctggg agtgggagct gaagccgaat atttttcgag ctgtatgaaa ttcctccgca
    13981 tatcaaaccg aactggcagg tgtggtacga gtgcgtgctg ccggaagatc gccagcatgc
    14041 cgaaaaagtg attcgtgatt cgttgcaatc acgctcgccc tttaagctgg aatttcgcat
    14101 taccgtaaaa gacggtattc gccatatccg cgccctcgcc aaccgggtac tgaataaaga
    14161 aggcgaagtc gaacgcctgc tcggcattaa tatggatatg accgaggtta aacagcttaa
    14221 cgaggcattg tttcaggaaa aagagcgcct gcacattacg ctggattcca tcggtgaagc
    14281 cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccagtgg cggagaagat
    14341 gagcggctgg acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac
    14401 ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc
    14461 gattgaacaa gatgtggtgt tgcactgccg gaacggcggc agttacgacg tgcattacag
    14521 tattacgccg ttaagtactc tggacggcag caatataggc tcggtactgg tgattcagga
    14581 cgtcaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact
    14641 gacgcatctc gccaatcgcg ccagttttga gaagcaactg cgtatcctgc tgcaaacggt
    14701 aaacagtaca catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt
    14761 gaatgacagc gccgggcatg cggcgggtga cgctttactg cgcgaactgg cgtcgttgat
    14821 gctgagtatg ctgcgctcca gcgacgtgct ggcgcgactc ggtggtgatg aatttggtct
    14881 actgttgcca gactgtaata tcgaaagtgc gcgttttatc gctacacgca ttatcagtgc
    14941 cgtgaatgat tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg
    15001 gattaccttg attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat
    15061 cgcctgttat gcctccaaaa atggtggacg gggccgggtg acggtttacg aaccacagca
    15121 agctgccgca catagcgaac gggcggcgat gtcgcttgat gaacagtggc ggatgattaa
    15181 agagaatcag ttgatgatga tcgcccacgg tgtcgcttcg ccgcggatcc cggaagcgcg
    15241 taatttgtgg ctgatttcac ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca
    15301 aaaatttcgt cgtagcttca gcgatccggc acttagccat gctcttgacc gacgggtatt
    15361 ccacgaattt ttccagcagg ccgcaaaagc ggttgccagt aaaggcataa gcatcgccct
    15421 ccccctttcc gttgccggtt tgagtagcgc cacgctggtg aatgatctgc ttgagcagct
    15481 gggaaatagc cctctaccac cacggttatt acatctgatt attccggctg aagcgatttt
    15541 agatcacgca gaaagcgtgc aaaaactgcg gctggtggga tgtcggatag tgctcagcca
    15601 ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct
    15661 acttgatggt gagttatgcg ccaacgtgca gggtaatttg atggatgaga tgctgattac
    15721 gattattcag gggcacgctc agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt
    15781 acccttagtg atggatacgc tttctggcat cggcgtcgat ctgatttatg gtgatgtgat
    15841 tgccgatgcc caaccgctgg atttgctggt gaatagcagt tatttcgcga ttaactgaga
    15901 gagcgtttgc ccattgcctg atgcgacgta tcaggcctac aattcgtccg ttccgtctgg
    15961 ttgccagcct tccgtatacc agatatgcaa cagcgcataa gaacgccagg gcttccagcg
    16021 ctcggcataa cggcggattt gtgccggtgt catccccgga aatcgctgtt taatcagata
    16081 atcatccggc agaaaaacat ctttcgcctg ccagccacgc aaagcgaaat aattcgccgt
    16141 ccagcgcccg atacccggaa aagtttgcag cgttttcatc gcctgctcca catcgcccgg
    16201 aattgtcatt ggtaagctgc cctccagcgc cgcatttgcc agatgaatca gtgcctctgc
    16261 ccgtttcaac ggcatgccta acgcttttaa tgcctgcggg tcggctgctg ccagccgctg
    16321 aggcgtgggg aagcagatat actccggaaa atcatccagc cgttcgccat aaagctgtac
    16381 aactttggca gtcaattttg ccgccatcgc cacgctcacc agttggccta aaatcgcccg
    16441 cacaccctgc tcaaaagcat caatacagcc gggtaaacgc aatccgggcc gcgccgcgcc
    16501 taacttgccc aacgcaccgt taacaatctg cgggttacat tgcagatcaa acaggcggct
    16561 cattttcgcc agacactcag cggcaacggg ttctaaacct gcacttaaat ttatgtgcag
    16621 agtatggcgg gctatatccg gaatagcagt caccacgccg cgatattcgc ccaccgccag
    16681 actacgggca taataatcgt cagcgaccgt ttccacacca ctcactgcac gggcggcgag
    16741 aaatcccaac atccacgacc agtcatacgg cggctgccag ttcagggtat acatcgcatc
    16801 tccttattca tccgctttca gcataaacgt tattcagacg ctacgctttg ctttcatatt
    16861 ccggttgtcg cgacggcaac atttcgctaa agtcacgccc cttcttcacc ggcatgggga
    16921 ttatttcgtg tttattggtt ttgattacgg tacagcaaac tgttcagtgg cggtcatgcg
    16981 tgacggtaaa ccgcacttgc taaaaatgga aaacgacagc acgctgctgc cttcaatgct
    17041 ttgcgcgcca acgcgtgaag cggtaagcga atggctgtac cgccatcatg acgttccggc
    17101 agacgacgat gaaacgcagg cgttgttacg acgggcgatt cgttataacc gcgaagaaga
    17161 tatcgatgtt acagcgaaaa gcgtgcagtt cggtctttcc tcactggcac agtacattga
    17221 tgatccagaa gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg ccagcggctt
    17281 aaaaccgcag caggtagcgc tgtttgagga tctggtctgc gcaatgatgt tgcacattcg
    17341 ccagcaggcg caggcgcagc tgccagaagc gattactcag gcggtgattg gtcgcccgat
    17401 caacttccag gggctgggcg gcgatgaagc aaacgcccag gcgcaaggga ttctggaacg
    17461 cgcggcgaag cgtgccggat tcagggacgt ggtattccag tatgagccgg tcgcggctgg
    17521 gctggattac gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg atatcggtgg
    17581 tggtacgact gactgttcat tgctgctgat gggaccgcag tggcgttcgc gtctcgatcg
    17641 tgaagccagc ctgctgggtc acagtggttg tcgtattggc ggtaacgatc tggatatcgc
    17701 cctggcgttt aaaaacctga tgccactgct gggtatgggt ggcgaaaccg aaaaaggcat
    17761 cgccctgccg atcctgccgt ggtggaatgc ggttgccatc aacgacgtac ctgcgcagag
    17821 tgatttctac agtagtgcca acggtcgtct gcttaacgat ctggtacgcg atgcccgcga
    17881 accggaaaaa gtggccctgt tacagaaagt ctggcgtcag cgtttaagct atcgcctggt
    17941 acgcagcgca gaagagagca aaatcgccct ttcaaacgct gcggaaaccc gcgcctcact
    18001 gccgtttatc agcgatgaac tggctacgct gattagccag caagggctgg aaagcgccct
    18061 cagccagcca ctggcgcgga ttctggaaca ggtgcaactg gcgctggata acgcccagga
    18121 aaaaccggac gttatatacc tgaccggcgg tagcgcccgt tcgccgctga ttaaaaaggc
    18181 tctggcagaa cagttgccgg gcattccgat tgcaggcggc gatgactttg gctccgtcac
    18241 cgccgggctg gcacgctggg cggaagtggt atttcgttaa aagaaaatgg ctcagcagcg
    18301 gcttaaatct gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg gttccatcaa
    18361 cggcatggtc cgtgctggag cataaaggcg gaatcgctgc aactctgtcg cgccgacagt
    18421 caacgccgat tccagccgat gtcgtttggc ggccattacc gcctgtctgt ctgctggcgt
    18481 taccgcttcg ttcggcctga acacaaaacg gcggtcacag ctcgctttcc agtcgataac
    18541 cgcctgggtc agatggtcgc caaacccttt aacttgctta acgccacgac gggtaacatc
    18601 cgctgctgtt tcaataccaa aagaccgtaa cgccgcttta cgcgcagggc caacgccggg
    18661 aatagaggca aaaaatccct ccagaaactt ctgcttctgc cgttcccttg cggtgtcata
    18721 aagtgctgcc agagcgcgtt tctcctcttc cggtaacccg agaatttcgt ccttcatttt
    18781 ttcgagcatc gcccgtttgg cgataaaacc ttccagcccg cccaactgtt ggatctggct
    18841 gactaaatgc tcatagtcca ttttcgcgcg attaaatgcc tcacggcgtt gctggatttc
    18901 tgctttgtac actttgcttg tcagactgcc aataatccag atagccgaca gcacaggaac
    18961 caaaataata taacgcggtt ctgcctgaag gccgcaaagc aacagcgata acgctgacag
    19021 tgcgatctca atcagaatga tgtattcgcg ccgtaacagg cctaaaggaa gcggcctgcc
    19081 agtcggttgg aaatgatcgg gtaatggcaa ttgcaatgct ggcggtgcta ctgacgccat
    19141 caccatcgcc cagactttcg ccagcacaaa atcaccgctg gtggtaatga cctcttcacc
    19201 gagatcaaca aaatagataa cgccttgatt atccagcgta caccacgggc agtcggtcaa
    19261 atgagcggga taaacatgca ttgccgaaac gggacatttc tttaactgtt ggcgcagaga
    19321 atcaagtgcc gctacccacg ctttagccgt cggacgcccg gttgccacgc cactttccgt
    19381 gaatgcctgc tgaaacatgg cttcaacatc actcggtaac atcgatagcg gaatcgatcg
    19441 tggcggcggt tttaaaccac gtcgctgatt atctgacgcg taggcataac ggaaatgggt
    19501 aatatccgtc tccagcgcat tacccgcatc agagataagc ggcacaccag aataaggatg
    19561 ccggccacca aacaagacgt gaaaaatcag caacgcaagg ccaaaattat cgtgattttc
    19621 tgtgcgttca aagccgacaa atgacgacaa ggtttgtagc tctggcggcg taaaatgcga
    19681 cacgccgact tcgcataaat gcagtgtgcc attgacgtta atctgaaagg agtcgctatc
    19741 aatcagtacc actttgctgt cgcgacctac cataaaactg ttctggttta cgtcacccac
    19801 gacgtgcccg tgctcgtgaa ccgtagcaaa agatgaagca atattgcgcg caacatagag
    19861 tagaaaatcc cacgcacaat gagggtaact ctggcgacga tgtgccgggc tatagatcat
    19921 atgaatcggt tctttaccag aaacttttgg catcataaaa ccgataactt ttccgcctcg
    19981 tccaccgtga agcgttgcct gcggccaggc gacataattc aacaactgcg cgtcagctgt
    20041 cgcagccata aaggcaagtt tgtcctgttt tgaggcgggt ggcggcgtgt gataaatctt
    20101 ggcgacgcta tcgacaaact cctcgatatc ataaaccgcg ccttcaccgc ctttgcccag
    20161 ttcacggcct ggcgtaacgc actcaccagt agcagtatat aaagtgggtt tcatggtaac
    20221 cacagcgcca gtgccaacgt tttatcatca tccgtacgct cgtttaccgc cgggctggac
    20281 aaaaactgct ttaacaattc aggtaataaa tcgagttgtt cctgcgttac tgacgccagt
    20341 ccattgaaaa acggggtaaa gaaaggcaca tgaggagaat tatccagcat atttaacgcc
    20401 agccgctgaa taccatccgt aaatgctgca actttatgcg cacgcccagt gctggtgaaa
    20461 gtttccagtc tggaaacggc atcttcgtcg gtaatgaagt gcgtcatgtt ggcatattca
    20521 cccaccatcg gcgtgagagg caattgcaga ccgtgaccca aatctaccac cacgccgcca
    20581 tcgccaatct gcataatcaa ggtgccgtca ggcgatgaaa tcaatcccag aaacgtgcag
    20641 gcaaaatcgc gaactgctaa tgctttggct tctgcctcgg caaaaagtcg ttgacgaata
    20701 gtcagtacca tatttgtggc gaggacatca ttaagcccca attcaccgtc ctgtactttt
    20761 tgcaccataa acgccatcgc ttcattgacg gcgagcgtcg cgccttctcc tccctgcgaa
    20821 acgctacctg cgccatcagc aaggaacatc accagtaatg gttgctggtc gtttaaccag
    20881 acaacctgca tctggcaggc atcctgacaa ggcaaatcag cactaatgtg cgatgttcca
    20941 acagcggatg cataaaccag acgccagctc acactgacgt ccagcctttt ggcgcttcca
    21001 gcacaacttc cgttcccggc gtggagcggg aaaccgaacg cagcgaactg gataaccagc
    21061 taaacagttc acggaattgt aatccctgca gtggcaaagg ctgacgaacg ctgatttgtg
    21121 ccagagtctt catatccgcc ccctgaacac cgatggaaaa gaaggcaaac ttcttatctt
    21181 cttccccctg aaacactttg ttggcggcag cctgccactc atcggttgga gcgccatcgg
    21241 tgatcaggaa aatccacggg cgataatacg aaataccatt tgcgcggtac tcacgtttcc
    21301 tctcttcaac catatctagg gctttggtaa tggcggcccc cataggcgta tccccctggg
    21361 caaacaggat gggcgggaaa aagttagcgg cgctggtaaa gggttgttcc acatgcaccg
    21421 ggccgaacgt cacaatgccg agttccactc tttttaaagc cagcggatca gcaagcagtt
    21481 catcacgaaa ggtaaccaat ccggcgttaa gttcattaat aggtctgccg ctcatcgagc
    21541 cagaaacatc cagcagcaaa atgcaggggc aacgtggttc cgggttactg gcaaaatcgc
    21601 tggtggcaaa cgtgatttgt tcgctcataa gaaatccttt cttagagaat tatcgaggtc
    21661 cggtatcata ccgattgcaa taatgaaata agagcaaacg aagaaacttt cagaattagg
    21721 cccgtcggcg cagaaaattt tgctattcgc ctcgcaagaa cggagagccg tcgattgaca
    21781 ctaagggcgg agtgacataa tttcaggagt gagggttagg gagaggtttc cccctccccc
    21841 tggtgttctt agtaagcctg gaagctaatc actaagagta tcaccagtat gatgacgtgc
    21901 ttcatcataa ccctttcctt attaaaagcc ctcttctccg ggagaggctt tcccgtttca
    21961 gcgtcccgct gaaatcatcg gcttacctcc tttcgccctg aatgcagtct atcgctaacg
    22021 cgtgaataaa atagtttcct ctgtgattat tgcgtaacga ctcgcaaaga atatttgctg
    22081 gcaggatcgc agactacaaa gcctac
//
LOCUS       DAJVOY010000057        21676 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVOY010000057 DAJVOY010000000
VERSION     DAJVOY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.057"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..798
                     /locus_tag="OQL90_001890"
     CDS             <1..798
                     /locus_tag="OQL90_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCS1368686.1"
                     /translation="LMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRL
                     LMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTF
                     TVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMS
                     WGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFS
                     YWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(842..1240)
                     /gene="psiE"
                     /locus_tag="OQL90_001891"
     CDS             complement(842..1240)
                     /gene="psiE"
                     /locus_tag="OQL90_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1368687.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSVAILLLVITLWLCNSKRLKRE"
     gene            1510..1788
                     /locus_tag="OQL90_001892"
     CDS             1510..1788
                     /locus_tag="OQL90_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368688.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1835..3931)
                     /locus_tag="OQL90_001893"
     CDS             complement(1835..3931)
                     /locus_tag="OQL90_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1368689.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIGGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3931..4668)
                     /locus_tag="OQL90_001894"
                     /pseudo
     CDS             complement(3931..4668)
                     /locus_tag="OQL90_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            complement(4665..5303)
                     /locus_tag="OQL90_001895"
     CDS             complement(4665..5303)
                     /locus_tag="OQL90_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1368690.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5417..5659)
                     /locus_tag="OQL90_001896"
     CDS             complement(5417..5659)
                     /locus_tag="OQL90_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368691.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6121..7770)
                     /gene="pgi"
                     /locus_tag="OQL90_001897"
     CDS             complement(6121..7770)
                     /gene="pgi"
                     /locus_tag="OQL90_001897"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1368692.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8295..9644
                     /gene="lysC"
                     /locus_tag="OQL90_001898"
     CDS             8295..9644
                     /gene="lysC"
                     /locus_tag="OQL90_001898"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1368693.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9896..10168
                     /locus_tag="OQL90_001899"
     CDS             9896..10168
                     /locus_tag="OQL90_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS1368694.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10301..11173)
                     /gene="rluF"
                     /locus_tag="OQL90_001900"
     CDS             complement(10301..11173)
                     /gene="rluF"
                     /locus_tag="OQL90_001900"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1368695.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11521..12468
                     /locus_tag="OQL90_001901"
                     /pseudo
     CDS             11521..12468
                     /locus_tag="OQL90_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            12538..13344
                     /locus_tag="OQL90_001902"
     CDS             12538..13344
                     /locus_tag="OQL90_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1368696.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTHG"
     gene            13354..13761
                     /locus_tag="OQL90_001903"
     CDS             13354..13761
                     /locus_tag="OQL90_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS1368697.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13761..14255
                     /locus_tag="OQL90_001904"
     CDS             13761..14255
                     /locus_tag="OQL90_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCS1368698.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14321..15118
                     /locus_tag="OQL90_001905"
     CDS             14321..15118
                     /locus_tag="OQL90_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1368699.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15129..15971
                     /locus_tag="OQL90_001906"
     CDS             15129..15971
                     /locus_tag="OQL90_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1368700.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            16005..17234
                     /gene="sorE"
                     /locus_tag="OQL90_001907"
     CDS             16005..17234
                     /gene="sorE"
                     /locus_tag="OQL90_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCS1368701.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17617..18306
                     /gene="pepE"
                     /locus_tag="OQL90_001908"
     CDS             17617..18306
                     /gene="pepE"
                     /locus_tag="OQL90_001908"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS1368702.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEDNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18397..20028)
                     /locus_tag="OQL90_001909"
     CDS             complement(18397..20028)
                     /locus_tag="OQL90_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1368703.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20248..21676)
                     /locus_tag="OQL90_001910"
                     /pseudo
     CDS             complement(20248..21676)
                     /locus_tag="OQL90_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog.; frameshifted; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
BASE COUNT         5145 a         5676 c         5656 g         5199 t
ORIGIN      
        1 ctgatgtcac gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat tatgggcaac
       61 acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg ccgcaaaacc
      121 ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat gctctccatc
      181 ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt gttcaaaacg
      241 ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt tatcaacctc
      301 accttcaccg ttctggcaat tatgacggtg gataaatttg gtcgtaagcc actgcaaatt
      361 atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt ttacactcag
      421 gcaccgggta ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt tgccatgtcc
      481 tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat tcgtggtaag
      541 gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc ctggaccttc
      601 ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt ctcctactgg
      661 atttacgggt gtatgggcgt tctggcagca ctatttatgt ggaaatttgc cccggaaacc
      721 aaaggtaaaa cccttgagga gctggaagcc ctctgggaac cggaaacgaa gaaaacacaa
      781 caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc gtgctgtctt
      841 tttactcccg cttcagccgt ttcgaattac acagccacag agtgatcacc agtaacagga
      901 tcgcaaccga gtagatcaac acatccagtg gcgatttatg atcgacgatg atcaagcgca
      961 caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa ccggactgaa
     1021 agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc agctcatatt
     1081 tgctggtttg ttctggcgcg aacagcacat ctgccagatg caccgtttct ttgccgagga
     1141 agacaaccaa aatcaggccg aggcacagca ggccaagatt gagtacggtc tgcaaaatgg
     1201 tggagataaa ctccacgcgc ggacgagaga gtgacgtcat agcagtacct ccttcacagt
     1261 ggcgagcttc ctgtataacg caaaaatgtg acggagatct atattttttg tgtatgtttt
     1321 gttcaacctg gatttggagc gcgcctccac caaagccgga tatacctacc cgtaggccgg
     1381 ataagccgcg cctggcggcg catccggcaa gacaaaccgc acccaagccg gtaattcaaa
     1441 aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga caatgttcct
     1501 ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta ttttgctgct
     1561 ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac tgctgataaa
     1621 gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgct caggcaacca
     1681 gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta cacaccagat
     1741 cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga atgaaggtgc
     1801 cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg ctacggtcgc
     1861 tggtcatgtc atacaactgg aacttacgcc caagttgctg accaccgtca cgcgtcagcg
     1921 gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga tccaacggta
     1981 ccgagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc aaaacgttgg
     2041 tgatcgtggc atagccaccc accaccacgc cgctgtcaaa gcgtttggcg atctccagcg
     2101 tgccgccttt atcccctgcc agatactgcc cgacgctggc tttcaccaac acgtcctggg
     2161 cgaaagatgg cgtccagtag gcggtcagat gtccggtttt cacgctgtag tcggtgaatt
     2221 tcatcatatc ttttgcacta cgccagtcgc gctgtttaac gtagttggca tccagaccaa
     2281 acgcccagtt actgtccagc ggacgataca acacttcggc ccccgcaccg ccaaacatgg
     2341 tttcgagata acctccatag acctgaccgt agaagccgtt gcccagatgc tggaagtagt
     2401 tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca tgggtacgaa
     2461 cgcgcggtaa gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg tagttgttgg
     2521 caagatttgc aaacaggctg ccggtggtca gcagatggtc cgtcagccac aaatctgcca
     2581 ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc gactggttca
     2641 gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat ataccagccc tgctcggtgg
     2701 actgcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg cccaacggct
     2761 ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc tgcggcatgt
     2821 taagacgatt ttccgtaatg cggatcgttt tgatcccatc cggcagatcg ttcatcacga
     2881 tccgattagc acggatgatc ccttcgcgcg aatcacgata tttcacctgc tcgccggtga
     2941 catacagcgt atcgcctttc gcctggatct gcggatcggc aagtccgaca ttgtatttca
     3001 acagcgttaa ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc ggctgcggtt
     3061 gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag ttggtgcgca
     3121 gcgtgacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg tcggcccaat
     3181 cggtaacgcg ataaatcgca ccgacgttaa acttactttt ttgctccagc ttcccggcaa
     3241 aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt tgccagggcg
     3301 tctggtattc cacgccgcca aacagtgagg caggaccgtg gaacatctgg ctaccgccga
     3361 tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg gctgaacaga
     3421 gcggattttt cacattaccg ctggtgccca gataacccca gcccaggccg agcgtaaaat
     3481 cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc cccgtaccgc
     3541 cgatatccct cgcaccaacc gccacttgcg gcagccagta actctcttcc cacaaacgca
     3601 gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaacgct tcgacgctgc
     3661 tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac gggaagagtt
     3721 gcactgaagc tgagtaataa cggtactgat cgttatcgcg atagttcagg ctcaactccc
     3781 cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg aaatccgact
     3841 gcgacgggcc aatgggcgca ggatatgttt cgccgtagca agccatgcta atgcccagcg
     3901 ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg tcagagtctg
     3961 aagaatgtcg gcgttaaggg catcaggcgt ctcactccag acggagtccg cgaggccaac
     4021 ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag ccaccggcgc
     4081 tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg caccgctaag
     4141 caggttctgc ccggagagat agctcgccac gtcgcgacct ggagtgaatg gctgcttgcc
     4201 aggacggctg ataagcccga acaacgtgac cgtggacggc ggtggtccga cccacagcgt
     4261 gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg cgcacgatat cgggatcaag
     4321 attgattttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta aggcgttaat
     4381 ggcggcggca tcgtcggcgc tggagtctgc ggcctgttca gccagccgtg tcagcaacgc
     4441 ctgctgctga cgcaatgctg ccgccgttgc cagctcttcg ctaatcaccg caccgggcca
     4501 ccagttgttt gccagccgcg gttagcctac cagatcgatt aaatgctctg cgcctgttag
     4561 tgttttggcc tcactgctgc cattgctgaa caccttgacg gttcctgccg cgaagaccga
     4621 tgacgctccc acactcaaaa gcaacgcgac aaaagtttgt ttaatcattg ttttgccgcc
     4681 ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc cgactggcga
     4741 atttgccctt cgctgtcgat ccagtagcgg ttatgccagc gcgcctggtc ggtggagact
     4801 tcttcgtcca gcacgcgaac caaggtttca tcgctgccga ctttgacggt atcggtgcca
     4861 tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc ggtccagccc
     4921 atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag cgggtcggcg
     4981 gcaaggttat tcacttcaat caggttgtcg ccgccaagca aagtcttcac cagacgacca
     5041 tgttgtgtga caagagtggc ttgatcctga gtgacccatt tttgttgtcc gtcttcagcg
     5101 aaggccagca ccacaaacaa ttgcggcccg ccattaagct gcatgtactg gctggcgtag
     5161 ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa cagactgtcc
     5221 cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag caggcagata
     5281 agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac cacaccggag
     5341 tgtggttatg gtaaatccct tcatacttca agccacagat gcaatgactc aaattatttt
     5401 ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc ccggtattag
     5461 aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt acggtgctga
     5521 cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct gccgcttcgc
     5581 ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg gcaaaaatgc
     5641 catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat ttacccaaaa
     5701 acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc gcggggaatt
     5761 agcgatggcg ttttaggata attggaatga atatcaaata taagcttcaa cttatattta
     5821 aataacaata aaaatcataa acttaaaata aaaacaaaag taattaatct gcgtattctc
     5881 cgaaataaac aacgattaaa aatctatttt aggattaacc tcacggtatg atttccgtta
     5941 aatcacagac aaggggattt atctgataaa acaatgccga cttattgtcg gcattgtttt
     6001 taaggttatt taactcctca tactatgcat taatgtcgat attggcatca ggctccggtt
     6061 gccggatgcg gcgtaaacgc cttatccggc ctacatatcg gcatcaggca cgcatgatga
     6121 ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat cgtggctgct
     6181 gatttctttg tcatctttca gctctggcag aatacggttc gccagttgtt tacccaactc
     6241 cacgccccac tggtcgaagg taaagatgtt caggatcacg ccctgagtaa agattttgtg
     6301 ctcatacagc gcaatcaacg cccccaggct gaacggagtg atttcgcgca gcaggatgga
     6361 gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa gcgttgccgg
     6421 atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac caaacgccag
     6481 cgcttcggtc tgggcgaaga agttagacag cagtttctgg tggtgatcag agagcgggtt
     6541 atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct ggtggatcag
     6601 ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc cagataatcg ggccagtctg
     6661 gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca tattgccctg
     6721 ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg cttcagtttc
     6781 cgcaccaaag aaattgttgt accagatgcc aatcagcgcc agcaggacag gcaggttttt
     6841 ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa gcagttcaac
     6901 gaagttatca aagccgatgg agagaacaat cgacaggcca atcgctgacc acaaagagta
     6961 acggccgccg acccagtccc agaactcgaa catgttggca gtatcaatac caaactcgcc
     7021 aacggctttg gcattggtgg aaagcgccgc aaagtgtttt gcaacgtgtt tttcatcacc
     7081 tgccgctttc aggaaccagt cacgcgcgct atgggcgttg gtcatagttt cctgagtggt
     7141 gaaggtttta gatgctacca agaacagcgt ggtttccggg tttacttttt tcagcacttc
     7201 agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat ttttgtacgg
     7261 acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccaccgatcc cgatgttcac
     7321 tacgtcagtg attgctttgc cggtataacc cttccactca ccggagataa tcgcttccga
     7381 gaaggttttc atcttctcca gcaccgcgtt gacttccggc attacgtctt tgccatcaac
     7441 cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc ggttttcagt
     7501 gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat cgcactcttt
     7561 cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg agtaatccac
     7621 cagcatctga tcgtcgaatg ttgcggagaa cttagaaaaa cgatcgccgt ctttagcaaa
     7681 aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg cctgccaggc
     7741 agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt tgagaattgt
     7801 gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt gccgttagcg
     7861 taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact actgaccgcc
     7921 aggagtggat gaaaaatccg catgaccccg tcgttgacaa ccgccccgct caccctttat
     7981 ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc aagtaacggt
     8041 gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg tgattgaacg
     8101 gctggccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca ccagaagcgt
     8161 tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt agcgaagtat
     8221 cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa tggatcccct
     8281 gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc agcgtagctg
     8341 attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac gtgcgtttag
     8401 tagtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagcg gaagggctgg
     8461 aacctggcga gcgattcgaa aaactcgacg ctattcgcaa catccagttt gccattctgg
     8521 aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg gagaacatta
     8581 ctgttctggc agaagcggcg gcgctggcaa cgtctccggc gctgacagat gagctggtca
     8641 gccacggcga gctgatgtcg accctgctgt ttgtcgaaat cctgcgcgaa cgcgatgttc
     8701 aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgaccgattt ggtcgtgcag
     8761 agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca cgcctcaatg
     8821 acggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt acaacgacgc
     8881 ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta cacgcatctc
     8941 gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc gtagtttccg
     9001 cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca acttttggtg
     9061 caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc ccggtctttg
     9121 tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa actgaaaatc
     9181 ctccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact ttgcacagcc
     9241 tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc gcgcggcata
     9301 atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc cttgatacca
     9361 ccggttcaac ctccactggc gatacgttgc tgacgcaatc tttgctgatg gagctttccg
     9421 cactgtgccg ggtggaagta gaagaaggtc tggcgctggt cgcgttgatt ggcaatgacc
     9481 tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt actggaaccg ttcaacattc
     9541 gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc ggcgaagatg
     9601 ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg tatggcctgg
     9661 aagctatatt tcgggccgta ttgattttct tgtcactatg cttatcaata aacgagcctg
     9721 tactctgtta accaacgtct ttatcgagaa taattgcctt taattttttt atctgcatct
     9781 ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata ttatcagttc
     9841 tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca ccatcatggc
     9901 actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac ttaaaacact
     9961 gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa ccaacagcat
    10021 taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga aaaaagcccg
    10081 ccaattgaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta gctggtcagc
    10141 aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac gcctgagatt ttcactcgtc
    10201 tgtgtggaca caatcttccc ctcaccctaa ccctctcccc gaaggggcga ggggactgtc
    10261 cgagctcttt ttggacgttt tcatcagtct gaggcgttaa tcagcgtccc ttctttttac
    10321 gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt tccgccgttt
    10381 tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttggcc ttcgctttag
    10441 gttttgcctc ggaagaggaa ttttcgatga gcttaaagag gtcgattaac tcatcgtcgg
    10501 ttaaatcgcg ccactccccc agcggaatgc cgcttaagct gacgttcata atgcgcgtac
    10561 gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc tgacggttca
    10621 gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact ttgcactttt
    10681 tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat tcatcggtaa
    10741 tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca cggaggatct
    10801 tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct ttatccaggc
    10861 ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta tcgcgctcgc
    10921 catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt accaaatctt
    10981 cggcttcccg aggctcaatc aactgaccgt ttactttcac aatgtcgccg ggtttcacct
    11041 gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg atatagcgat
    11101 ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa cggactgatg
    11161 agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct tagcggggaa
    11221 aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt ttatcacaaa
    11281 agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat aaggataatc aacatattac
    11341 gaatcgctgc acacctgtgc tgatgactac tacgcagaga aacaaatgtg caagagaaag
    11401 cgctatatct ccccatgcaa agagtgaaaa atcgctttga aaggccctca tgatggggtt
    11461 atatcagatc aagcggtgag aaagcatatt tgcacaaatg tgcagaacca ggaggcggca
    11521 atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta ttacgaacag
    11581 gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccac catcagccgc
    11641 ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta tgactacaac
    11701 gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga cgttgtggtg
    11761 gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt acacggcgcg
    11821 caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg gggtcgcgcg
    11881 gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat cgcggcagtt aatctgcgta
    11941 ccaattattg gcggaccgtc cggtaaactc gaaagccgct atcatgtaaa cacattaacc
    12001 tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg cggattttcc ggctctgctg
    12061 gataacccat taattcgtaa tgggatcatg cagtctcagc actttaaaac catctctgcc
    12121 tactgagata atctggatgt cgccctggtg ggaattggct caccggccat tcgcgacggc
    12181 gctaactggc atgcgtttta tggtggtgaa gagagtgacg acctgaatgc ccgccaggtt
    12241 gctgacgata tttgctcgcg cttttttgat attcacggcg caatggttga aacgaatatg
    12301 agcgaaaaaa cactctctat cgaaatgaat aaattaaagc aggcacgata ttccattggc
    12361 attgccatga gtgaagaaaa atacagcgga attgttggtg cactgcgtgg aaaatatatt
    12421 aattgtctga taacgaatag cagcacagct gaactattac tgaaataaaa atcttcccga
    12481 tgtttagcgt aataaaaatt acggcggggc cctattatct aaaatcagga gtctgttatg
    12541 caaacgtggt taaatttgca ggataaaatc attattgtca ccggcggcgc atccggtatt
    12601 ggtctggcga ttgtagagga attattagca caaggcgcaa acgtacagat ggtagatatt
    12661 cacggtggcg atggtcaata tgaaggtcat aaaggctatc agttttggcc gaccgatatt
    12721 tccagcgcca aagaggtaaa tcatacggta gcggaaatta tccagcgttt tggtcgcatc
    12781 gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga tgagaaagcg
    12841 cctgccgggc agtatgaact caacgaagct gcattcgaaa aaatggtcaa tatcaaccag
    12901 aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga tggtcaaaca acatgatggc
    12961 gtgattgtga atgtttcctc agaaagtgga ctggaaggct cagaaggcca aagctgttac
    13021 gccgctacca aagccgcgct taatagcttc acgcgatcct ggtcgaaaga attgggtaaa
    13081 cacggtatcc gtgtggtcgg tatcgcgccg gggattctgg aaaaaacagg actgcgcacg
    13141 ccggaatatg aagaagcgct ggtgtggacg cgcaatatca ccgtcgagca gctgcgtgaa
    13201 ggctatacca aaaacgccat tcctattggg cgcgccggaa gattagcaga agtcgctgat
    13261 tttgtttgtt atctgctgtc tgaacgcgcc agctatatca ccggagtaac cactaacatt
    13321 gcgggcggca aaacgcacgg ctaaggaggc tttatggtca atgccatttt ttgcgcccac
    13381 ggcaaattag cttgcgccat gctggaatcg gttcagatgg tgtacggcga cgccagggtc
    13441 gaggcagttg aatttgtgcc cggcgagaac gccggagaca tcgtagcaaa gctggaaaag
    13501 ttagtaagca ttcacaatca agatgagtgg ctgattgccg tggatttaca gtgcggcagc
    13561 ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc gtctgcgggt aatcagcggc
    13621 ctttctctgc cgctggcgct cgaactggtt gataaccagg acagcatgaa tgttgatgaa
    13681 ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg ttgtctggaa acaactggca
    13741 acggcagagg aggatttctg atgaacatta cgctcgcccg cattgatgac cgcctgatcc
    13801 acggtcaggt cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt attatctgca
    13861 atgacgaagt ttataacgat gaagttcgcc ggacattatt gcgtcaggct gcgccaccgg
    13921 gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc tgtttatcac aacccgcaat
    13981 atcaggacga aaccgttttt tatcttttca ctcgcccaca ggatgcctta gcgatggttc
    14041 gccaaggggt aaaaatagac accctaaata ttggcggcat ggcctggcgg cccggtaaaa
    14101 aacagttaac caaagcggtt tcattagatg atgatgatat taacgcattt catgagttaa
    14161 ataatcttgg cgtcatactg gatttacgcg tcgtagcgtc agatccctca atcaatatta
    14221 tcgacaaaat aaacgaacag ttaatcgcaa attaaataat agcgcttgtg tacatatgct
    14281 ttaacaggca gggatgacga cacttaaaag gtgaaaaatc atggaaataa gtaccctaca
    14341 aatcattgct atatttcttt tttcctgtat tgccggaatg ggcagcgtgc tggatgaatt
    14401 tcagactcat cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg gtgatttaaa
    14461 aaccggaata atactcggtg gtacgctgga attgatagct ctcggctgga tgaacgtcgg
    14521 cgcggcgcaa tctccggatt ctgcactcgc cagcataatc tccgccattc tggttatcgt
    14581 tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg cctgtggctg cggcaggcca
    14641 ggtgctgact gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg cggataaagc
    14701 agcggaagaa gcacggtttc gcactctcga tattctgcat gtctccgcgt ttggcgtgca
    14761 ggcgctgcgc gttgctattc cggcactgat tgtctcactg ttcgtcagcg ccgatatggt
    14821 gagcaatatg ctgagcgcga taccggaatt cgtgacgcgc ggtctgcaaa ttgctggcgg
    14881 ttttatcgtg gtggtcggtt acgccatggt gctacgtatg atgggcgtga aatatttgat
    14941 gcctttcttt ttcctcggtt tcctcgcagg tggctacctc gatctcagtc tgctggcgtt
    15001 cggtggcgtc ggcgtgatca tggccctggt ctacatccag ctaaatccac agtggcgtaa
    15061 agctgaacct caactccagg ccaccgcctc caccgccctt gaccaacttg acgattaacg
    15121 gagccatcat ggaacagaga aaaattacac gcagcgatct ggtgagcatg tttctgcgct
    15181 ccaacctgca acaggcgtcc tttaactttg aacgtattca cgggctgggt ttttgctacg
    15241 acatgatccc cgccatcaaa cgactttacc cattaaaaga ggatcaggtt gcggcgctca
    15301 ggcgacacct ggtgttcttc aataccacgc cagccgtatg tggcccggtc atcggcgtca
    15361 ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt accattaacg
    15421 gcatcaaagt cggtctgatg ggaccattgg caggagttgg cgatccactg gtctggggaa
    15481 cgctgcgccc gattaccacc gcgctcggcg catctctggc actttcgggc aacattctcg
    15541 gcccgctgct gttcttcttt attttcaatg cggtgcgtct ggcgatgaag tggtatggcc
    15601 tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg aatgtgctgc
    15661 aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg ctggtcacca
    15721 aatggacgtc aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc gatggctcca
    15781 ccgtcaccat gaccgtgcag aacattctcg accaactttg ccctggtttg ctggcgctcg
    15841 gtctgacgct gcttatggtt cgtctgctca acaaaaaaat taacccggta tggctgattt
    15901 tcgccctgtt tggcttaggg attatcggta atgcgctggg cttcctgtcc agattcttcg
    15961 ccccggcatg actgccgggg ccatcgctca acatgaggtg gtttatgaaa acaacagctc
    16021 tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt tgaccttcct gaaatgcagg
    16081 aggatgaaat cctcgcgacg gtggtcactg acagcctgtg cctctcttcc tggaaagagg
    16141 ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac cccatcatca
    16201 tcggccacga gttttgcggc gatattctgg ccgtgggtaa aaagtggcag cacaaattcc
    16261 agccgggtca gcgttatgtg attcaggcca acctgcaact ccccgaccgc ccggactgcc
    16321 ccggctactc cttcccgtgg gtgggcggcg aagccacgca tgtggttatt cccaacgagg
    16381 tcatggaaca agattgcctg ctggcatacg acggcgaaac ctattttgaa ggctcactgg
    16441 ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt caggaaggta
    16501 gttataacca cacgatgggg attcgcccgc aagggcgcat gctgatcctc ggcggcaccg
    16561 gaccaatggg actgttggcg attgattatg cgctacatgg acccgttaac ccgtcgcagc
    16621 tcgtcattac cgataccgac aacgataaat tgagttatgc gcgcaagcac tatccatcag
    16681 aaccgcaaac actgattcat tatctcaatg ccgccgatgc agcatttgat acgctaatgg
    16741 cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat gaaggactgg
    16801 tgactctcgc ctcttccttg ctggcgacag atggttgcct gaatttcttc gccggaccgc
    16861 aggataaaca tttcagcgcg ccaattaatt tctacgatgt gcattatgca tttacccact
    16921 acgtgggcac gtcaggcggc aataccgacg acatgcgcgc ggcggtcaaa ctgattgaag
    16981 agaaaaaagt gcaggccgca aaagtggtaa cacatattct tgggctgaat gccgcgggcg
    17041 aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat accgggaaat
    17101 acctgccgct gacgtcactc acgcagattc aggatcaagc actggcggcg attctggcgc
    17161 gtcatcaggg gatctggtcc ggtgaggcgg agcaatatct gctcactcat gcagaggcaa
    17221 tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg ccgcggcaac
    17281 ttcgcgacct gatgtgcggc tgattgctga agcggcccaa tgtgcacgag aaggataaaa
    17341 aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg tttatgccgg
    17401 atgcggaata aagccttatt cggcctacat aatctcgaaa attcaatata ttgcagttat
    17461 ctggtaggcc tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc agtctcagct
    17521 cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc gcagtggtag
    17581 catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt attgagtaac
    17641 tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc tgaacagttg
    17701 cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac ctgggatgat
    17761 tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac cggtattcat
    17821 agcgttgtcg atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt cggcggcggg
    17881 aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggcacc aattactgac
    17941 gtggttaaac gtggtgcgct gtatattggc tggagcgcag gcgctaacct tgcttgccca
    18001 accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga tgcgctaaat
    18061 ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg ccataaaggt
    18121 gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact gacgattatt
    18181 ggtctaccgg aagataactg gatcacagtg agtaaaggtc acgctacgct gggtggcccg
    18241 aacaccacct atgtgtttaa ggccggtgaa gaagcggttc cgctggaagc tggtcaccgt
    18301 ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct gatgcgacgc
    18361 ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc gtccttcatc
    18421 ttcatccgac tgttccagca cactgtaagc caccgaacaa aacagcgagt tcaggcgctg
    18481 catatctccc agtaagccta aatgtagcga actggtttca atgctctgca cgttttgctg
    18541 atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa agcgatgctt
    18601 actacggcgc aaacgacgag cgctggtgac atcgccagag aagaacaccg acattgccag
    18661 ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc catcaagcga
    18721 aaatgcccgc cgtgctgcca gtgatttgtc agcaatttcg ctgcccatgc gctcgacgat
    18781 atcggaggcc tgttcaaggt tgagcgacat ttcaataatc tccgcccagc ggcgcgactc
    18841 ttcctccgcc agctcttctt ttggcatccg cgccagatac agcttaatgg cggtgtagag
    18901 aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct caccgtgcat
    18961 cactttattc aacccttcca tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg
    19021 cgcggcgttc gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca gatgtttagg
    19081 ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa accgtgccat
    19141 cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga cgtggaaata
    19201 gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca gcaaatggac
    19261 aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca gcgcgacacg
    19321 gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt
    19381 agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg cggtcagagc
    19441 cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca tcgcgccaat
    19501 cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc
    19561 ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa tcagcccaag
    19621 accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat
    19681 cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga gaatacgcgc
    19741 cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg cgagggctac
    19801 cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc tctgtaccag
    19861 tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag
    19921 gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa tatgagtccc
    19981 ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga gttcaggttc
    20041 tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa acacgctgac
    20101 tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt ccagcctgat
    20161 tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg ccctgtttgt
    20221 tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat tcggtgccag
    20281 ccagcgctca acatctgaga cgctcatacc tttacggcgg gcatagtctt caacctgatc
    20341 gcgctgaatt tgcgccacgg cgtagtactt gctgtccggg tggctgaaat accaacccga
    20401 aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg tatgtttttc
    20461 cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg ccgggtagcc
    20521 cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc tgaggttctc
    20581 gttcggcgca tagccccagt agactttacg cacacgctca tggagatact ccgcaaaggc
    20641 ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat cgtgctgcgc
    20701 ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat
    20761 gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt agttagcgaa
    20821 tcccgttttt tcggtctgtt gacgcagatg gtggctgaca ttgatcacat gggtgcgcgt
    20881 ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca ggcccaccac
    20941 gccacgccac gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg
    21001 tctttaaaca gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgcggatac
    21061 ttcccggcca gcgaccaggt cataaagaac ggtgtccagt cgatgcaatt acgcagagtt
    21121 tcgatgctgg cttcaacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc
    21181 tgccagtcaa aagcgaagtc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgc
    21241 ggtttcttgc gcccgtgctg aatacgtacg gtttcgtact ccttgcgggt acgagcgaca
    21301 aaatcatcgc gctgagtatc ggaaagcagc gccgccacca caccaacggt gcgcgaggca
    21361 ttctgcacat acaccgtcgg gccgctgtag ttctgctcaa ttttcaccgc cgtgtgcgct
    21421 tttgaggtgg tcgcgccacc gatcagtaac ggaatggtga agccctgacg ctccatctct
    21481 ttcgccacgt taaccatctc gtccagcgac ggcgtgataa gccccgaaag gccaatcaaa
    21541 tcagcattca cttctttagc agtacggaga attttttccg caggcaccat cacgccgaga
    21601 tcgacaattt cgtagttgtt acattgcagc accacgccaa cgatattttt accgatatcg
    21661 tggacgtcgc ccttca
//
LOCUS       DAJVOY010000058        21457 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVOY010000058 DAJVOY010000000
VERSION     DAJVOY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.058"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..392
                     /locus_tag="OQL90_001911"
     CDS             <1..392
                     /locus_tag="OQL90_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1368704.1"
                     /translation="HYPLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEI
                     DEKFMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKPEHPLPSI
                     KLYISYNKSSLNNLVFSRFIDRLNESF"
     gene            539..2056
                     /locus_tag="OQL90_001912"
     CDS             539..2056
                     /locus_tag="OQL90_001912"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCS1368705.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEDDRIKAINEIRMAIHQ
                     VSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK
                     NAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQIT
                     AMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2057..3206)
                     /gene="ybdL"
                     /locus_tag="OQL90_001913"
                     /pseudo
     CDS             complement(2057..3206)
                     /gene="ybdL"
                     /locus_tag="OQL90_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3315..4403
                     /gene="hcxA"
                     /locus_tag="OQL90_001914"
     CDS             3315..4403
                     /gene="hcxA"
                     /locus_tag="OQL90_001914"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS1368706.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4413..4610)
                     /locus_tag="OQL90_001915"
     CDS             complement(4413..4610)
                     /locus_tag="OQL90_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1368707.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4792..6897)
                     /gene="cstA"
                     /locus_tag="OQL90_001916"
     CDS             complement(4792..6897)
                     /gene="cstA"
                     /locus_tag="OQL90_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS1368708.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7078..7491)
                     /gene="entH"
                     /locus_tag="OQL90_001917"
     CDS             complement(7078..7491)
                     /gene="entH"
                     /locus_tag="OQL90_001917"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS1368709.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7494..8240)
                     /gene="entA"
                     /locus_tag="OQL90_001918"
     CDS             complement(7494..8240)
                     /gene="entA"
                     /locus_tag="OQL90_001918"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS1368710.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8240..9097)
                     /gene="entB"
                     /locus_tag="OQL90_001919"
     CDS             complement(8240..9097)
                     /gene="entB"
                     /locus_tag="OQL90_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS1368711.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9111..10721)
                     /gene="entE"
                     /locus_tag="OQL90_001920"
     CDS             complement(9111..10721)
                     /gene="entE"
                     /locus_tag="OQL90_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS1368712.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATASPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10731..11906)
                     /gene="entC"
                     /locus_tag="OQL90_001921"
     CDS             complement(10731..11906)
                     /gene="entC"
                     /locus_tag="OQL90_001921"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCS1368713.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12281..13237
                     /gene="fepB"
                     /locus_tag="OQL90_001922"
     CDS             12281..13237
                     /gene="fepB"
                     /locus_tag="OQL90_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1368714.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13241..14491)
                     /gene="entS"
                     /locus_tag="OQL90_001923"
     CDS             complement(13241..14491)
                     /gene="entS"
                     /locus_tag="OQL90_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS1368715.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14602..15606
                     /gene="fepD"
                     /locus_tag="OQL90_001924"
     CDS             14602..15606
                     /gene="fepD"
                     /locus_tag="OQL90_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS1368716.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15603..16595
                     /gene="fepG"
                     /locus_tag="OQL90_001925"
     CDS             15603..16595
                     /gene="fepG"
                     /locus_tag="OQL90_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS1368717.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16592..17407
                     /gene="fepC"
                     /locus_tag="OQL90_001926"
     CDS             16592..17407
                     /gene="fepC"
                     /locus_tag="OQL90_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1368718.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17404..18537)
                     /gene="fepE"
                     /locus_tag="OQL90_001927"
     CDS             complement(17404..18537)
                     /gene="fepE"
                     /locus_tag="OQL90_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1368719.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18752..>21457)
                     /locus_tag="OQL90_001928"
     CDS             complement(18752..>21457)
                     /locus_tag="OQL90_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCS1368720.1"
                     /translation="EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLP
                     GEYAQLAQINATQVEIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALA
                     NLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDAR
                     PSLLITTDDQLPRFADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRP
                     KGVMVGQTAIVNRLLWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAE
                     PEAHRDPLAMQQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEAL
                     PADLCREWQQLTSVPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGL
                     RILDAMMHPVPPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVA
                     RWLDNGAVEYLGRSDDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGG
                     DARQLVGYLVSQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKAL
                     PLPELKAQAPGRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQL
                     SRQVARQVTPGQVMVASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPA
                     SGFAWQFSVLSRYIDPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPY
                     YLLGYSLGGTLAQGIAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEI
                     NREREAFLAAQQGSTSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEG
                     MSPERAWSPWIAELDIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
BASE COUNT         5075 a         6087 c         5490 g         4805 t
ORIGIN      
        1 gtcattaccc tctaatttgt agtaataagc atccgcgaat tactgacaat agcacttatg
       61 aacaaattat ggcagaagag ttcacccaat taatatccaa atcagcagga gttgatgata
      121 ttcaaatgga aatagacgag aagtttatga atcgcaaaat ttcatttcgt ggttcctctt
      181 tgctgacaat aattaatagc attgcagtta cagatttact gggaatagtc ccctatgaac
      241 tttataactc ccaccgtgac tttcttaatt taaaagagat aaaaccagaa catccgttac
      301 cttcaataaa gctttacatt tcctataata aatcttcttt aaataatttg gtgttctccc
      361 gatttattga ccgtctaaat gagagttttt gatatataac tacagacagc tactataact
      421 ttcatctatt tattcacagc gcgaatgaaa aataaaacac tctgctttac agtgtttact
      481 ttataattaa cagtcttatc tcactgttca ccctgtaacg ttcagagagc gtcttcagat
      541 gtctatttat aaaattcctc ttccgctcaa tattctggaa gctgcacgag agcgtattac
      601 ctggacactc aacaccctac cccgcgtttg tgtctcattt tctggcggca aagactctgg
      661 tttaatgctg catctgacag ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt
      721 gtttatcgac tgggaggcgc aattctcttg cactattaac tatgttcagt ccctgcgcga
      781 gttgtacacc gatgtcatcg aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa
      841 ttccctttca caataccaac ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg
      901 tcagcccccg caagatgcga taaccgatcc tgactttttc tgcttttacc agccaggcat
      961 gaccttcgaa caatttgtac gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc
     1021 gatgatgatc ggcatccgtg cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt
     1081 aaataaacaa cgttttgccg acgataaacc atggactacg gccgcaccag gcggtcatag
     1141 ctggtacatt taccccattt acgactggaa agtggctgat atctggacct ggtatgcaaa
     1201 tcatcaaagt ctctgcaacc cactgtataa cctaatgtat caggcaggcg ttcctctgcg
     1261 tcatatgcga atttgcgaac cttttggccc ggagcaacga caaggattat ggctctatca
     1321 cgttatcgaa ccggatcgct gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg
     1381 cggcatttac gccggacatg acaatcattt ctatgggcat cggaaaatcc tcaagccaga
     1441 acatttagac tggcaagaat atgcattatt gctgctcaat agcatgccgg aagatgatcg
     1501 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1561 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1621 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1681 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1741 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1801 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1861 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1921 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1981 gcgcctgaag caaattaacg gcctgcaaga gttgtttgcc gaccgtcaat attcccgcgc
     2041 ctggacggtt aaatagctaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     2101 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     2161 acagcggaat cgccgccacg ccgtgctccc gcgtcagcca ctggcaaaac tcaacatcat
     2221 ccagggtaga aacctcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2281 tctccagccg gctttcattt aaagcattca ccagaatatc gcgcttctgg cgataaaagt
     2341 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2401 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2461 gcgctggcgc aacacaataa cccactttcc agccggtcat atgatagatc ttgccaaatg
     2521 aagaaaccgc cactgcccgc tcacgcagct gcggatgcgc cagcacactg gcatggccct
     2581 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2641 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggggttat
     2701 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2761 agtcaaggtg gttgcagtgc catacgcttc actattcccc cagaaagcgc gatggcgggg
     2821 gcgtaactgt catagctggg atcaaaacaa atcacttcat cgccattgcg caccagtgcg
     2881 gtaatcgccg cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc gctatcggca
     2941 tctggttgat agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc
     3001 acgccggtca tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc
     3061 tgtaaatagc gcggaccatc aaaatcagga aagccttgcg acaggttaat cgcctggtgt
     3121 tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg aagtttgctt
     3181 tgtggaatca gagggttatt tgtcattttt attgtacctg acgaagttgc gtgttgaagg
     3241 cactataaca cgatgctagt atttggcaat caagacgttt agatgtctaa ataaaacaat
     3301 aaggacaaca caacatgcct cacaatccta tccgcgtggt cgtcggcccg gctaactact
     3361 tttcacatcc aagaagtttc aatcacctga acgatttttt cactgatgaa caactttctc
     3421 gcgcggtgtg gatctacggc aaacgcgcca ttgctgcggc gcaaaccaaa cttccgccag
     3481 cgtttggact tccaggggca aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg
     3541 tacagcaact ggcggctgag tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg
     3601 gcgcactgct cgacaccgcg aaagccctcg cccgccgtct cggtctgcca tttgttgccg
     3661 ttccgacgat cgccgctacc tgcgccgcct ggacaccgct ctccgtctgg tataacgatg
     3721 ccggacaggc gctgcattat gagattttcg acgacgccaa ttttatggtg ctggtggaac
     3781 cggagattat cctcaatgca ccgcaacaat atctgctggc ggggatcggt gacacgctgg
     3841 cgaaatggta tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc
     3901 ggctggggat caataatgcg caggccattc gcgacgtctt gttaaacagt agcgaacagg
     3961 cacttgccga tcagcaaaat cagcagttaa cgcaatcatt ttgcgatgtg gtggatgcta
     4021 ttattgctgg tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg
     4081 ctcatgccgt gcataacggt ctgactgtcc tgccgcaaac cgagaagttt cttcacggca
     4141 ccaaagtcgc ttacggaatt ctggtgcaaa gcgccttgct gggtcaggat gatgtgctgg
     4201 cgcaattaac cggggcgtat cagcgttttc atctgccgac tacgctggcg gagctggaag
     4261 tggatatcaa taatcaggcg gagatcgaca aagtgattgc ccacaccttg cgcccggtgg
     4321 agtccattca ttacctgcca gtcacgctga caccagatac gttgcgtgca gcgttcgaaa
     4381 aagtggaatc gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc gccttttcca
     4441 ccgtagcgcg cgtcctgccg ctcacggaaa aactcctcgt aggtcatcgg cgtttgatcg
     4501 ggatggttaa cccgcatatg ttcgacatag ttgtcgtaat caggcatacc aatcatcagc
     4561 ttcgccgcct gacctaaata ttttccggct tttgccagtg aatcaaacat cttggcctcc
     4621 gctaaccatg ttgctgaaag agtgccggat gcggcgtgaa cgctttatcc ggcctacaaa
     4681 tgctatcaac gcactcggtc agtcccctcg ccccatcggg gagagggtta gggtgagggg
     4741 gaaccctcgg cactatgcca acattcgcca acatcccccc tcactctgac tttagtgcgc
     4801 gcctttcgcc tgcgctacga tctcctcgac attttccggc atcggctcat acggcgtttc
     4861 tttcgccgtt ggcttcggat ctttcaatgc cgcaagtgcc gtcttaatcg agaacagtgc
     4921 cagaaccacc acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa
     4981 caccagttgc gccagctgtg actcagtata ctgcgacgga atattgccgc tgtcgatcat
     5041 tgcctggaac ttgttagcaa tggccaggaa gccgactttc gcatccgggc taaacgcttt
     5101 ctgccagcca gcggtcaggg tacaaatcag cagccaggcc gttggtacca gcgccaccca
     5161 ggcgtaacgt tgacgtttca tcttgaacaa caccacggca cagagcatca gcgccatccc
     5221 tgccagcatc tggttggcaa taccaaacag cggccacaga gtgttaatac cgcccagcgg
     5281 atcgaccacg ccctgatgga ggaagtagcc ccacgccagt acgcacagcg ccgttgccag
     5341 caggttagca ggcagtgaat cggtccgctt caggccagga gacaccacgc ccagcagatc
     5401 ctgcaacata aagcgcgcag cacgcgtacc cgcatctacc gccgtcagaa taaacagagc
     5461 ttcaaataga atggcgaagt gataccagaa cgccacatcc atcataccac ccagcgcacc
     5521 gtgcaggatg taagccatcc ccaccgccaa cgtcggcgca ccgcccgcac gggaaatgat
     5581 cgactgttca cccacttcgc tggcaatctg gttgagcgta tctggtgtaa tgctaaagcc
     5641 ccagctactc accacctgcg cggcagaagc gaccacatcc gccgtccctg ctggagccag
     5701 caccgccatt gggctgttca tggcaaagta aacgcccgga tcgatgatac aggcggaaac
     5761 cagcgccata atcgccacga aggattccat taacattcca ccgtagccga taaaacaagc
     5821 ctgcccttcg ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg catggaagcc
     5881 agacaccgcg ccacaggcga tggtgataaa caggaacggg aacaggttac cggtccatac
     5941 cgggccagtg ccatcgacaa atttggtcag cgcaggcatg gtcagcgtcg ggcgcataat
     6001 caaaatgcct accgccagac cgacgatcgt cccgattttc aggaaggtag agaggtagtc
     6061 acgcggggcc agcagcaacc acaccggcag caccgccgcc acaaaaccgt aacccaccag
     6121 catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg gactttctgc
     6181 cacccagccg ccagagataa tggcgaaaat caggaatacc agaccgatga ccgacacttc
     6241 accaatacgc cccggacgca gatagcgcag gtagatcccc ataaacagcg ccagcggaat
     6301 ggtgaacgca acggtatacg ttccccacgg gctatgagtc agggctttca ccacgatcat
     6361 cgccagtact gcaaggataa tgaccatgat cataaagcag gccaccagcg ctatcacccc
     6421 ggcggttggc cccatctctt ctttgaccag ctcacccagc gatcgaccgt cacggcgcgt
     6481 agaaacaaac agcaccatga aatcctgcac cgcaccggcg agaaccaccc cggcgagtag
     6541 ccagatcatc cccggcaggt agcccatttg cgctgccagc accggcccaa ccaacggacc
     6601 tgctccggcg atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata
     6661 gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc ttcgggtcaa ccgccagcac
     6721 atttttggcg atatacaacc cataaaaacg gtaagcgatc agatagatac agaccgacgc
     6781 caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt atcccagagc
     6841 aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt
     6901 tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgtacg
     6961 atttaacaac acggtaacta tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt
     7021 taactcgatc actccgttaa ccgagttttc ctgcaatctc atttaattct gtcgggctca
     7081 tcccaaaact gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac
     7141 gacgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga cgccgcgtac
     7201 cttgccttca gacaccgggc gatgatgggt ggcgttaagt tccgtgccca ccacacactg
     7261 cccgtcacgg gtcatcataa atccggccat cgatcccagc gtttcagcca gcgccgccga
     7321 cgcgccgcca tgcagcaggc caaacggctg atgagtacgg gtatcaaccg gcatttcggc
     7381 ttccagcaca tcatctccca gacgggtata cacaattccc agatgcgcca ccattgtgtt
     7441 atcgctggtg gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc
     7501 cccagcgttg agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgaggtca
     7561 gaggcgagga acaaaatcgt gttggcgatc tcttgtggac gggcgatttt ccccagcgga
     7621 atgccgagtt taaactgctc gccaaagccg cgaatacgct gttcttcggc gtcatcgctc
     7681 acccacagcg tgcgttgcat atcggtgtcg gtggagccag gcgaaaccac attacagcgc
     7741 acgccgctac ccgccagttc cagcccgacg ctcaacgcca ggcttttcag cgccgctttc
     7801 gatgcgccat aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga cgccacagtg
     7861 acaatcgccc cgccccgctg acggcgaaac tggttcatgg tttgctggaa caggttaaac
     7921 gcaccgccga cgttaaccgc aaaagtctgc tgccagtcct ctttactgag ctgatcggtc
     7981 gcgcccatgc gcaaaattcc cgccgcattg atcagcacgt ccagtcgctc cgtttcagct
     8041 aacagtcgct gacatacctg cgccacctgc ccggcgtcgg caacatccat tacttcggtc
     8101 gcaaagggat attgctcctg agtgaacgct tgatcaaaac ctgtaacttt cgctcccgcc
     8161 tcaacaaacg ccagcgccgt ggcgtagccg atacctttac ctgcgccggt tacccagaca
     8221 tttttaccgc tgaaatccat tatttaacct cgcgggagag tagcttccac caggcgtcga
     8281 tggtcgggtt tttcgccagc atgacaaagt cgatatcacc atgcactttg cgccagcgcg
     8341 ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga
     8401 acggttcatc ggactcgtcc agcaacggca ggataacctc acgcagcgcc gctttgctgg
     8461 cggggacagg tgctggcagt aattcttcag tcatcaccac ccggccagac cgcccggcca
     8521 catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tccgccacca
     8581 taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg
     8641 catatacccc ggtaataatc agctggttac gtccactctc tttgagcatt tgctccagcg
     8701 gagaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg
     8761 tcaggcgatc caccaccttt tgctgttccg gcgaacgggt caggcccggc ccccacatat
     8821 cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg taataaactg
     8881 ggatattgtg ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc acctgctcca
     8941 tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca tggattaaca
     9001 acgcggcacg ttgcagttca aaggcccagt caactttatt ctgcggaata tcgtgagact
     9061 ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgat
     9121 gcgcgtgacg ccagccactg acttaattgt tttttatcga ctttcccgac cgccgtcagc
     9181 ggaagtgaat ctacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca
     9241 cgcaggaaac gacgcacctg caccgcgcgc agcggctctt ttaccaccag ataagcacag
     9301 cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcatagat caccgccgga
     9361 tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcgccgcc acggttgatc
     9421 tgatctttct cgcgcccctg cacggtgatg taaccctctg gatcaataga gatcagatcg
     9481 ccggaacagt aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg acttttgtaa
     9541 taaccgcgga aggtgtacgg cccgcgcgtc atcaggcgtc cgacttcccc ttgcggcagt
     9601 ggatttcctt cggcatcggc aacccacact tcatcgtccg gacacattgg gtaaccctgg
     9661 gtatggataa ttttctccgc gctatcatca agacgggtgt agttcaccag cccttccgcc
     9721 atgccaaaca cctgctgcaa ctggcagcca atctcagcgg gaatacgcgc cgcaagggtg
     9781 gcagaaagac gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg cgcccggctt
     9841 tcgccttcag ccagcgcctg caaccacagg ctgactgccg gcggcaccag cgcggtgacg
     9901 ttcacctgat gtttttcaat caatgggaag caaagcgtag cgctgggatc ggccgccaga
     9961 acaaccgttc ctccggcaag aaagacgccc agcgatcctg gcgaactcat ggcgtagtta
    10021 tgagccgccg ggatcgcgca caggtagcgc gtctgttgtg tgaactgaca aatctcgacg
    10081 ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagctt cggtgtaccg
    10141 gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggtgatgc cgtggcagta
    10201 aaaccgtctg ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc
    10261 agttgcacca cgcgaatgga ggaatgttct gtgacgaaag tattgaggaa atcatccccg
    10321 ctaaacagcg catgttggcg atcggcaatc agcaatgcgg gttcaatctg gctggcatag
    10381 gcgttcagtt cactacgctg atggctgaac aacgccagca ccggcgcaac gcccagtttc
    10441 agcagcgcga aaaaggtaat atacaattca gcgacgttac ccagttgtac cagcgcggtt
    10501 tcaccaggtt taatgccctg acggcgtaaa gtacacgcga ggttatccgc cgcctgattc
    10561 agctcccggt aactcaactg tagctcgccg tcgataaccg cgatgctgtc actcgcagcg
    10621 tggcgagtca gaatgtcggt cagcggcaaa tcctgccagt agcctttttc ccgatagcga
    10681 cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat
    10741 ccaaaaacat tcaacatggt agaaagtttg acgcctgttt cgcgccactc acccaacggt
    10801 gacgacgcaa gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc
    10861 gcgcagcgga tggtcaccac ccattcgccg ttaccttcgc tgtcacacca acccacaatg
    10921 ccgccaaaca gttcgcggtc gaacggctcc agttcagcaa taacctgagt cgcggcctga
    10981 tgcgggaaac cgctcagcgc gggggtcgga tgcagcagac aggccagagt cagtgcgttt
    11041 tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggta
    11101 atcaattgtg gagaggaggg aacgtgtaac tcactactgc gttcgcgcag tacctctttc
    11161 atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta
    11221 cccgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc taacggaatg
    11281 gagctaaaac gctcgccgtc tttacgtagc agcagttccg ggctggcccc cagcaggacg
    11341 ccaccatcag ccagcgggac atggaagttg taactaaccg ggttttgcgc aatcaaccgt
    11401 tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa ccgtgacaac
    11461 accactttgt cgacctgcgg cgtggcggta agtgcggcag cgcgggcaac catctgttca
    11521 aacgtggttt gttccggaat tgcctggcgt tccaccacat tcagcgactg gctgcgggtg
    11581 aaacggcggg cggaagcttg tttttcctga cgggagaacg actgccagga ttcaggaata
    11641 tacaacgacg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac cggatttttg
    11701 atgccctgca ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg
    11761 ggcgaatccc cgttcacagc cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa
    11821 ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt
    11881 acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa
    11941 tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct
    12001 tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat gaaaatgaga
    12061 agcattattg atggattcgc ataagcgcaa tgtgatgtcc tgcgccgttc ttccccctct
    12121 cccttccagg gagagggctg gggtgagggt caatattcgc accagtgctg gctgctccca
    12181 tcaccctaac cctctcccca aaggggcgag ggtacggata gtgcgctttg tcgaatttgt
    12241 cattacgccc ttaaccttat taataacagg aagctgattt gtgagactcg ccccgctcta
    12301 ccgcaacgcc cttctattaa caggactttt gctttccgga atagccgcag ttcaggccgc
    12361 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    12421 gcgtattgtt tccaccagcg tcaccctgac cggctcactg ctggcgattg atgctccggt
    12481 gattgccagc ggcgcgacca cgccaaataa ccgcgtcgcg gatgaccagg gctttttacg
    12541 ccagtggagc aaggtggcga aagaacgcaa actacaacgg ctctatatcg gcgaaccgag
    12601 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    12661 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    12721 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    12781 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    12841 caaattaccg ccgcagccgg tcactgctat tgtctatacc gccgctgcac acagtgccaa
    12901 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    12961 gaaattgccc acagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    13021 gcttagtgga gaaaatctgg ctgcagggtt aaatggcgag tcactattcc tgttcgccgg
    13081 tgatcagaaa gacgccgatg cgatttatgc caatccactg ctcgcgcacc tgcccgcagt
    13141 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    13201 catgcaagtg ctggaacgac ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    13261 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    13321 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgctc
    13381 ccagaccgcc taacagcgcc gcgcctatgg catcgcccgt cacgttttgt gccgtccaca
    13441 aaccgttgat tcgccctaac attgcttccg gcgtttgcgt ttgcagcatt gtatattgca
    13501 gcaacgaact gaccgcactc agccagccga acagcgccag acaaaccacg cctaaaatcc
    13561 acatcggcat caggccaaat aaaccaatgg cgaggaacga tcccaacgtg gagagcaaca
    13621 tcaacaatcc tggtcgcgcg ctatgcgcca gtttcccact ggttaatgcg ccaatagccg
    13681 caccgagcgg gatcgccgca tagagaaaac caatctctgc cgccgacatc tgccagttgt
    13741 cagccagcgc cggatacagt acccgcaccg cgctcgccat cgtcaataaa ccacccagca
    13801 acgcaatccc gcccaccagc gggctggcga gcagaaaacg aaatcctgcc agtaatgatt
    13861 tcaacggatg ctcgcgcggc tgcggtggcg gtggcaacgc cggaaggctt aacaacggta
    13921 gcaaggtaat aaacgtgccc gccgccgcca gcccgtagtt ccaggctacg ccaccggtcg
    13981 ccagcaataa accgccaatc atgggcgaaa tcaccgaccc cagacgcacg gtcaacatgg
    14041 tgatcgcccc ggcctgcatt aagttttcac gccctaccag tgctggtgtc gccgccaata
    14101 gcgccgtaac gccaagcgat gcgaaaaaac catcccataa accaagtaaa tagattgcca
    14161 gcaatgacgg ctccggcagc agtgcattaa ggcacagtcc aatgaagcca atgccacagg
    14221 tgccgcgcgc cagcaaaatc acttttttgc gctcatagcg atccgccagc acaccgccaa
    14281 ccatcaggcc aacaaacatc gcgccgccgg tcagcgtcac cgaaagcccc accagccagg
    14341 tagaatgcgt catcatctgg atctgcaccg gcaccgcgac gccgagcaaa cccagagaca
    14401 caattgagat aaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt ttcaacaggc
    14461 tgaggttaag cagccaggat tgtttattca ttacaatgcc ttgccatcaa atattttaaa
    14521 tacctattct taggctgcac atgctaacat atccaaataa gatcgataac gataattaat
    14581 ttcattatca tggaagttcg tatgtctggt tctgttgccg tgacacgcgc cattgccgtg
    14641 cccggattgc tgttattact gattatcgcg acggcattaa gcctgctcat tggggcaaaa
    14701 tcactccccg cttccgtagt gctggaggcc ttctccggca cctgccagag cgccgactgc
    14761 accatcgtgc tcgacgcgcg actgccgcgc acccttgccg gtttactggc aggcggcgcg
    14821 cttggccttg ccggggcatt gatgcaaacc ctcacccgaa atccacttgc cgaccccggc
    14881 ttgcttggcg tgaacgccgg agccagcttt gccattgtgc tgggtgcggc gctgtttggt
    14941 tactcatccg cgcaggaaca actggcgatg gccttcgccg gggcgctggt ggcctcattg
    15001 attgttgcct ttaccggcag tcagggcggc gggcagttaa gtccggtgcg tttaaccctg
    15061 gcgggcgtgg cgctggcggc agtgctggaa gggctgacca gcggcatcgc cctgcttaat
    15121 cctgacgtct acgatcaact gcgtttctgg caagccggtt cgctggatat tcgcaatcta
    15181 cataccttaa aagtggtgct gatcccggtg ctaatcgccg gagcaaccgc gctattactg
    15241 agccgcgcgc tgaacagttt aagcctcggc agcgacaccg cgacggcgct gggcagtcgc
    15301 gtggcgcgca cacagttaat tggtctgctg gcgattaccg tgctttgtgg tagtgcgaca
    15361 gcggtagttg gcccgattgc ctttattggc ctgatgatgc cgcatatggc gcgttggctg
    15421 gtgggtgccg atcatcgctg gtcgctgccc gtaacgctgc ttgctacccc tgccctgctg
    15481 ctgtttgccg atatcatcgg gcgggtgatt gtgcccggtg aactgcgcgt ttctgtggtc
    15541 agtgccttta ttggtgcacc ggtgctgatc ttcctcgtgc gacgtaaaac gcgaggtggt
    15601 gcatgattta cgtctctcgc cgattactca tcacctgttt gctgcttatt tccgcctgtg
    15661 tggttgcagg tatctgggga ttacgcagcg gtgccgtcac gctggaaacc tcgcaggtat
    15721 tcgccgcgct gatgggcgat gcgccgcgca gtatgacgat ggtggtcacc gaatggcgtt
    15781 taccacgcgt gctgatggcg ctgttgattg gcgcggcact gggcgtcagc ggtgcgattt
    15841 ttcagtcgct gatgcgtaac ccgctcggca gccctgacgt aatgggcttt aacaccgggg
    15901 cgtggagcgg cgtgctggtg gcgatggtgc tgtttggtca ggacctgacg gctatcgcgc
    15961 tggcagcaat ggtgggcggc attgtcactt ctctgttggt ctggctgctc gcctggcgta
    16021 acggtatcga cacctttcgg ttgattatta tcggcatcgg cgttcgcgcc atgctggtgg
    16081 cctttaatac ttggctgttg ctgaaagcgt ctttagaaac ggcgctaaca gcgggtttgt
    16141 ggaatgccgg atcgctcaat ggcctgacgt gggcaaaaac ttcgccttcc gcacccatca
    16201 ttatattgat gctcattgcc gccgccttgc tggtgcggcg aatgcgcttg ctggagatgg
    16261 gcgatgatac cgcctgcgcg ctgggcgtca gcgtcgagcg ttcgcgtctg ttaatgatgc
    16321 tggttgcagt ggtgcttacc gctgcggcaa cggcgcttgc cgggccgatt tcctttattg
    16381 ctttagtcgc gccgcacatt gcccgacgca ttagcggcac cgctcgctgg gggctgaccc
    16441 aggcggcgct gtgcggagcg ctgttactgc tggcggccga tctctgcgcc caacaactgt
    16501 ttatgccgta tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt atttacctta
    16561 tcgtcttgtt aattcaggag tctcgcaaaa aatgaccgaa tcagtagccc gtttgcgcgg
    16621 cgaacagtta accctgggat atggcaaata taccgttgcg gaaaatctga ctgtagaaat
    16681 tcctgatggt cacttcacgg caattatcgg accaaatggc tgtggtaaat ccacgttact
    16741 gcgtacctta agccgcctga tgacgcctgc tcacgggcat gtctggctgg atggcgagca
    16801 cattaaacat tacgccagta aagaagttgc acgccggatt ggtctgttgg cgcaaaatgc
    16861 taccacgccg ggcgatatca ccgtgcagga gctggtggcg cgtggacgtt atccgcatca
    16921 accgctgttt acccactggc gcaaagagga tgaagaagcg gtaacgaaag cgatgcaggc
    16981 cacgggaata actcatctgg cagatcaaag cgtggatacc ctttctggcg gacaacgcca
    17041 gcgagcgtgg atcgcgatgg tgctggccca ggaaacggca attatgctgc tcgacgaacc
    17101 cacgacctgg ctggatatca gtcatcagat tgatctactg gaactgttaa gcgaactgaa
    17161 ccgcgagaaa ggctataccc tggcggcggt gctgcacgat cttaatcagg cctgtcgtta
    17221 cgccagccat ttgattgcat tgcgggaagg gaaaattgtt gctcagggag cgccgaagga
    17281 gattgtcact gctgaactga ttgagcgcat ttatggtctg cgatgcatga tcattgacga
    17341 tccggtagcc ggaacgccgc ttgtggtgcc gctcgaacga acggcacctt caaccgcaaa
    17401 tagttaaact aagtggtctg ccatcatggc atcctgtttt ctggatgcca tcgcatagcg
    17461 caataacacg ctaccacaag ccaccatccc gccgatcagc gcggaaagga tcacaataat
    17521 cgccttaccc ggaccgtctt ttttcactgg caatgacggg cttaactgat atttaaacgg
    17581 cgtaaaattc acatcgttga catttgcttt tgttaattgc tcgacaagat actgccgatt
    17641 acgtaattca ccgttcagtt ccgcaacgtc agtaaccgct ttttcaattt ccagtttgcg
    17701 ttcaataccg tctgcgccga gagaaataga gaaatcgggg tcatctttaa cggcctgacc
    17761 attactgtat acgggctttt taattcctgc cgcgttggca atgtcgagtg aataattgag
    17821 gcgctgaatg tttgcatcaa gttgattttt cgttttaatg cgatcctgag ccagtttttc
    17881 tttttcaaac tgggttttga tctccagttt attacggacg ttttctatcg actctttcac
    17941 caccaacgca gagatataat cgatataccc gctcaaaacg gtctgcgcct ctttactggt
    18001 tggcgcggta aaacttagcg tccaggaggt atacagtgac ggttcatctt ttttcttact
    18061 ggcattgtca tcaaccgctt tcattttttc gctcaatgca acaatagcgc gatgcaaatc
    18121 cagttcatcg attttcgcct cttttaattg gtccatcaca taaggtgatg aacgcaggta
    18181 ctcttccagc aagctaacag actgaaactt cttgataaac aggttaaatg caccgccacg
    18241 gtcgatatta acatcgagat caagaacgcg gagtttggta aaggttttct ccaggtcctg
    18301 ccactgaatg gcttcggcgg gagtaataac ggctgagctg gtccattttt gcggcaggat
    18361 gaaagagatc agtaaacctg cgcaggcaaa tgcaaaaacg accgccatga ccgttttttt
    18421 ggcccgccat aaaacttcga ttagattaag taaatcaatt tcattactac tgggcgacgc
    18481 cagaggatac tcggaaaaat gagcgtcact tccctgttta atattcagtg atgacatggg
    18541 tataacctga aaatgagtcc aataggggga aatctccacg gcgctcactt taccagttgg
    18601 tgagaattat ccgaagctga agtttgtaaa ttccttccac tgaatgcaac attcactcta
    18661 tttaagagaa atattcatca aacctataaa tcaaacaaat aaaaaaccca taattatttt
    18721 ctgtaattat gggtttatag ataatattaa tttacctgtt tagcgttgcg cgaataatcg
    18781 gcccaatttt tacaaacgcc cctggagaga taatatccac atgcgcacaa tcctgacgat
    18841 agatatccag ctcggcaatc cagggcgacc aagcgcgttc ggggctcata ccttcctgaa
    18901 gcgtgcgttc agcaacaaac agcgtcgctt taccatcaaa cggtacgcta tgagcagtcg
    18961 tcagcagacg cacagcatca gcgtagttgc cttcaatggt ggtaaacaac tccgttgaag
    19021 tacttccctg ctgtgccgcc aggaaggcct cgcgttcgcg gttaatctcc gccagcactt
    19081 ccgggtccag accattagcc tctttttcct gccagttttg cgtttctggc ggccaggtat
    19141 ccagcaaacc aagaaatgcc acctgttcgc cacgggcacg cagtcgcgcc gcaatgccct
    19201 gcgccagcgt accgccaagg gaatacccca gcaggtaata agggccgtgc ggttgttgtt
    19261 caagtaacgt tgccagatgc gcttcacaga cttcatccag atttgccgcg gtatgcatgg
    19321 ggccattggg gcgcggcgac tgaatgccga taatcgacca ttgtggatcg atataacgcg
    19381 agagcacgct aaactgccag gcaaaaccgg atgccggatg gaaacaaaac agcgtcgggc
    19441 cattaccttc acgcaacggc agaatggttt cgaatcccat acgccgggtg ctgtcttctt
    19501 cagcatcaat aatcgttgcc agtttagcga cggttgacgc gaccatcact tgccccggcg
    19561 tcacctggcg ggcaacctgc cgacttaact gcgctgccag tttcattgcc agtagcgaat
    19621 gaccaccaag cgcaaagaaa tcagcatcgg catcctgcac gtcacaaccc agcaacgacg
    19681 cgaatgccgc ggcaataatc gtttcactgc ccgcttttgg cgcacgcccc ggcgcttgtg
    19741 ccttcagttc aggcaacggt aaggctttgc gatccagctt gccgttggcg ctaagaggta
    19801 acagtggaag ttgcagcaga accacgggca ccatatgtgg tggcaatgtt tcgcgaagct
    19861 gcgcctgtaa tgcgctggta tccaacggca gacctgattg cgacaccaga tagcccacca
    19921 actgacgcgc atcaccaccg gtggctgccg cctggttaat cacacaggcg tgggtaacgg
    19981 cttgttcgac atccggcagc gcctgcatca cgcgatcgat ttcgcccagt tcaatacgct
    20041 gcccgcgaat ttttagctga tcgtcactgc gcccgaggta ctccaccgcg ccgttatcca
    20101 gccagcgggc aacgtctccg gtacggtaca tccgttcacc agggacaaaa ggatcggcaa
    20161 taaagcggct ggcggtcaga tcgggtcgtc caagataccc ctgcgccagt tgaatgccgg
    20221 tgagatagag atcacccgcc acacccggcg gcaccggatg catcatcgca tcaagaatac
    20281 gcagacccgt attccatacc ggataaccaa tcggcacact gctgccgcgc acctgtgcca
    20341 gttcctcgcc aaaagccgga taccagctga catctaccgc cgcttccgtc gggccatata
    20401 gattatgcaa cggcacgctg gttaactgtt gccattcgcg gcataaatca gccggtaagg
    20461 cctcaccact acagaaaacc tgtttcaacg tcgcgcaact ctggcgagcg gtttgcggcg
    20521 tcagcgatgc aacaaatgct gccagcatcg acggcacaaa gtgcgtggtc gttacgccat
    20581 attcggcaaa gaattgctgc atagcgagcg ggtcgcggtg cgcttccggt tcagccatca
    20641 ccagtttagc cccggcaata aacggccaga aaaactccca caccgagaca tcaaaactgc
    20701 acggcgtttt ttgggcaacg acatcttcgc ctgtaagcag ataatgattt tgcatccaca
    20761 acaggcggtt aacgatagcc gtctgcccga ccattacccc tttcggcctg ccggtggagc
    20821 cagaggtaaa gatgatataa gccgtgtgat gcggttgtga aagttgcagc ggcgcactgc
    20881 cctgcggttt aagcggggcg ttatagcaaa ggttggttaa atctggaaca tcggcaaagc
    20941 gcggcagttg atcgtcggtg gtgattaaca gcgacggacg cgcatcttcc agcatcattt
    21001 tcaggcgatc gtccggatag ccggtatcca gcggtagcca ggccgcgcca gcttcaacta
    21061 tcgcatgtag cgccagggtc aaaaagaccg agcgcggtag cgccaccgcc acgctgtcgc
    21121 ccggtttaac gccacgctca cgcagcagat tcgccagcgc caccacctgc tcgcgcattt
    21181 cccgatagct gaactggtaa cgcgcatctg ccagcgccgg agcatccggt gtttttgccg
    21241 cttgttctgc caccagcgcg ctcagcgtgg tttctggaat ctcaacctga gtggcgttga
    21301 tctgcgccag ctgcgcatac tcacctggca gcataatatc gacatcgccg cacaacagcg
    21361 ccggatccgc ggcgaactgg gcaatcagca ttttcaggcg ttcagcatgc tggattaacg
    21421 ttggctcatc gtaacgctgt ttattggcga ggatctc
//
LOCUS       DAJVOY010000059        20755 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVOY010000059 DAJVOY010000000
VERSION     DAJVOY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.059"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(11..556)
                     /gene="hemG"
                     /locus_tag="OQL90_001929"
     CDS             complement(11..556)
                     /gene="hemG"
                     /locus_tag="OQL90_001929"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1368721.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OQL90_001930"
     CDS             complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OQL90_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1368722.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2058..2672)
                     /locus_tag="OQL90_001931"
     CDS             complement(2058..2672)
                     /locus_tag="OQL90_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1368723.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OQL90_001932"
     CDS             complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OQL90_001932"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1368724.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4193..6382
                     /gene="fadB"
                     /locus_tag="OQL90_001933"
     CDS             4193..6382
                     /gene="fadB"
                     /locus_tag="OQL90_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1368725.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6392..7555
                     /gene="fadA"
                     /locus_tag="OQL90_001934"
     CDS             6392..7555
                     /gene="fadA"
                     /locus_tag="OQL90_001934"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1368726.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OQL90_001935"
     CDS             complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OQL90_001935"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1368727.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OQL90_001936"
     CDS             complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OQL90_001936"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1368728.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10154..10642
                     /gene="rfaH"
                     /locus_tag="OQL90_001937"
     CDS             10154..10642
                     /gene="rfaH"
                     /locus_tag="OQL90_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1368729.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OQL90_001938"
     CDS             complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OQL90_001938"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1368730.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OQL90_001939"
     CDS             complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OQL90_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1368731.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OQL90_001940"
     CDS             complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OQL90_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1368732.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OQL90_001941"
     CDS             complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OQL90_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1368733.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OQL90_001942"
     CDS             complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OQL90_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1368734.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OQL90_001943"
     CDS             complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OQL90_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1368735.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OQL90_001944"
     CDS             complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OQL90_001944"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1368736.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OQL90_001945"
     CDS             complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OQL90_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1368737.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OQL90_001946"
     CDS             complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OQL90_001946"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1368738.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18806..19618
                     /locus_tag="OQL90_001947"
     CDS             18806..19618
                     /locus_tag="OQL90_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1368739.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            20012..20672
                     /gene="hcp"
                     /locus_tag="OQL90_001948"
                     /pseudo
     CDS             20012..20672
                     /gene="hcp"
                     /locus_tag="OQL90_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5080 a         5604 c         5211 g         4860 t
ORIGIN      
        1 ttctttatgc ttatttcagc gtcggtttgt tggttaactg ggcgatttct cgggcgaaat
       61 tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg
      121 acatcttcat aatcagctta atcataaaac ggtcgtacca gcgataacgt gggtaacgca
      181 gcgccccggc aatgaccgcg cagcgatcgg gacgccattg tgaactcatc agaaacttgc
      241 gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc aggtttacgg
      301 aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg ttttttgaca aattcctgga
      361 acgcagagtg gtagtgacca tagcgaatag aagcaccaat gaccacacgg tcatagtttt
      421 cccattgtgg ttcttcaatg cggtgcacat tggcgacatc cgtctggatc cccagttctt
      481 tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa
      541 gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg taaagagcac
      601 cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga tccatttagc
      661 caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc ccaggttatt
      721 caatgtcgca acaactgagg caaaggcaga aaaatcatcc acgcccgtgg cgataatcgc
      781 cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa cggcttcgag
      841 gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt tcggatgcac
      901 cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga tcactttcag
      961 gccaccgccc gttgacccgg cgcaaccgcc gataaaagct gaacataaaa gcagtaccgg
     1021 caaaaagagc ggccagcggg caatgctgtc agtcgtaaac ccggcggttg tcgccatcga
     1081 taccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga cattatgaaa
     1141 ccacagtacg agtgtacaaa taaccaccag cgtaaactgt acgccgataa acatgcgaaa
     1201 ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa agtgcagacc
     1261 gtagttacaa ccggagatca gcaggaagat agcaatgata gtgttaatag tcggactgtc
     1321 gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag tcgcaaagct
     1381 atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg cacaggcgac
     1441 ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc gcgggcgcat
     1501 tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc cacccacgcc
     1561 gaggataggc agtatcgcaa ccgctaacac gatgatcccc atcccgccaa accattgcag
     1621 catctggcga taaaagagga tggcatgagg gagcgaatcc agccccacca gcgtagtggc
     1681 cccggtggtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg
     1741 gctttccgag aagataaaag ggagcgcacc gacactaccc agcacggtcc aaaacagcac
     1801 cactatcaga aacccctcac gggatttcag ttcgcccttc tctttgcggt tcggccacca
     1861 cagcatagag ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc
     1921 atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa ataagatgac
     1981 cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt ccttagttct
     2041 gcaaaatgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa
     2101 tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac ccgcagcaga
     2161 acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag caacgcttca
     2221 atgccggtta actgactata ttcacattgc aaagtatatt cggttaatgg cgtcttgcgt
     2281 tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt cactaaccca
     2341 ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagcggtaat ttccccgatg
     2401 ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcccgccgg ctccccgtcg
     2461 tcagagaaac ccagctgttg agaatcatcc ggcgcacccg ccacccacgc cacgcaatgg
     2521 tggcgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc cgcctcaacg
     2581 ccatcggtat gcgccaacat cgttatgaag cggctctttt tgatctcttc aacgaccgtg
     2641 accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc cgggtcatgt
     2701 tttccacgtt attttcgtgg atcaccacgt tgtcttcgat acgaatgccg ctgaacggtt
     2761 tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca cgccacggtg
     2821 ccagtagcga ttcgatgaag tagatacccg gttcgatggt taacaccatg cccggctgga
     2881 gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgt gtaccgctat
     2941 catcctgcat aaaaccggct acgtcatgca cctgcaggcc cagcggatgg ccgataccgt
     3001 gcggcataaa cggtccggtg agatcgtttt cgaccatcgc ttcttcactc atatcggtga
     3061 tgatttgatg tttacgcagc aatttggcga tgcgctgatg gaactggagg tggtaatcca
     3121 catagctgac gccagctttc atggtagcga tcagcgcaag ttgttcatca tttacgtctt
     3181 tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca ggtacgagtc aggtcagccg
     3241 catagccgtt atattcggcc ccggcatcca gcaggaagct gcgcatctct tccggtgcct
     3301 gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc gttaagcgcc acaatgttgc
     3361 tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcgatg ttgatatcga
     3421 actcgctcat gccagaacgg aacgcttctt ctgccgcacg atgaccgttg accgccattt
     3481 tctgcgcttc acgcatacag gccagttcgt actcggtttt gaaggagcgg tagtaatgca
     3541 ggtagtcgat cacccctttc gggttgatgt tgctggcctc aatacccagt tgcagcgcac
     3601 gttccggcac cggaccgata taaccgatat tgccgcgcgc agcgggcagc aggctaccaa
     3661 tgccatcggc tttcggcagt gcgatcactt ccacatcttc agtccagaag gaggtcggca
     3721 gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc ggcttgttca
     3781 cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac gctttgaatt
     3841 gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacgttgaac agctcgccgg
     3901 agtgaataag taatgcatcc agcttgaagc gcgccagcgc atcgcgagtc cgttcttgta
     3961 aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac ccttctgttt
     4021 ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga tttctgccga
     4081 gcgtgatcag atcggcattt ctttaatctt ttatttacat gtttttaaca caaaatacac
     4141 acttcgatgc atctggtacg accagatcac cttgcggatt caggagactg acatgcttta
     4201 caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac tggtatttga
     4261 tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg gcgaggccat
     4321 cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga acaaagcagc
     4381 ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca
     4441 gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag atctgccggt
     4501 gccgaccatt gctgccgtca acggctatgc gctgggtggt ggctgcgaat gcgtgctggc
     4561 gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa ccaaactggg
     4621 catcatgccg ggctttggcg gttctgtacg tatgccacgt atgctgggcg ctgacagtgc
     4681 gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga aaatcggtct
     4741 ggtggatggc gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg ttttacgcca
     4801 ggccattaac ggcgacctcg actggaaagc aaaacgtcag ccgaagctgg aaccactaaa
     4861 actgagcaag attgaagcca ccatgagctt cactatcgct aaagggatgg tcgcacaaac
     4921 ggcggggaaa cattatccgg cgcccatcac cgcagtaaaa accattgaag ctgcggcccg
     4981 ttttggtcgt gaagaagcat taaatctgga aaacaaaagc tttgtcccgc tggcgcacac
     5041 caacgaagcc cgcgcactgg tcggcatttt ccttaacgat caatatgtaa aaggcaaagc
     5101 gaagaaactc accaaagacg ttgaaacacc gaaacaggcc gctgtactgg gcgcaggcat
     5161 catgggtggc ggcattgctt accagtctgc gtggaaaggc gtaccggttg tcatgaaaga
     5221 tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca
     5281 gcttgaacgc ggcaagatcg atggtctgaa actggctggc gtgatctcca cgatccaccc
     5341 aacgctcgac tacgccgggt ttgaccgcgt ggatgttgtg gtagaagcgg ttgtcgaaaa
     5401 cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc cggataccgt
     5461 actggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc tggaacgccc
     5521 ggaaaacttc tgcggaatgc acttttttaa cccggtacac cggatgccgt tggtagaaat
     5581 tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct gggcgagcaa
     5641 gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta accgcgtgct
     5701 gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt tccgcaagat
     5761 cgacaaagtg atggaaaaac agtttggctg gcctatgggt ccggcatatc tgctggatgt
     5821 cgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct tcccgcagcg
     5881 gatgcagaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc gctttggtca
     5941 gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc cgaagaaaga
     6001 agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc gcgatttcag
     6061 cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg tccgctgtct
     6121 ggaagagggc attatcgcca ctccggcgga agcggatatg gcgctggtct acggcctggg
     6181 cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta gcgcaaaata
     6241 cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc cggaaggtct
     6301 gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag cccgtccggt
     6361 tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg tcgatgcaat
     6421 tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg cagaagatct
     6481 ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag cggcggccct
     6541 cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta atatcgcccg
     6601 taacgcggcg ctgctagcag aagtgccgca ctctgtcccg gcggttaccg tcaatcgctt
     6661 gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga ctggcgatgc
     6721 gcaggcatgt ctggttggcg gcgtggagca tatgggccat gtgccaatga gtcacggcgt
     6781 cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga tgggcttaac
     6841 ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg cctttgccgc
     6901 gcggtcacac gcccgcgcct gggccgccac gcagtcgggc gcatttaaaa atgaaatcat
     6961 cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg acgaggtgat
     7021 tcgaccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg atccagtaaa
     7081 cggtacggta acggcgggca catcttctgc actttccgat ggcgcagctg ccatgctggt
     7141 gatgagtgaa agccgcgccc atgaattagg tcttaagccg cgcgctcgtg tgcgttcgat
     7201 ggcggtcgtt ggttgtaacc catcgattat gggttacggc ccggttccgg cctcgaagct
     7261 ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaga tgaacgaagc
     7321 ctttgccgcg cagatcctgc cgtgcattaa agatctcggg ctaatggagc agattgacga
     7381 gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat ccgctgggct gttccggtgc
     7441 gcgtatcagc accacgctgc tgaatctgat ggaacgcaaa gacgttcagt ttggtctggc
     7501 gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg tttaaccact
     7561 tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc ctgcaccaat gtaggcctga
     7621 taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg acatagatct
     7681 aaatgccact gaataaagtt ttagttgccg ttcttcccgc ctgtcagggg cgggtttttt
     7741 tatatctcag ataaatgcaa acgcatcgcc aaacagacga tcttcccgcg cattacgctc
     7801 actgcaaaac aggtcacgag caattttcgc catctcaaaa cgtccggcaa tatagatatc
     7861 atgctctgcc agcgtaccat gatcctgcaa gaccgccgtt aacacggtgc cagtacgccc
     7921 acgccagccc gcttccggtt gttcaaccac cggcaccaca cgcaggccag gatgcttcag
     7981 cgaaagcgcc tcaagctcgg agagatcata aagatgttgc tcctcacgcc caccccagta
     8041 aatggtgata tcgcgattgg gattacgcgc caacgccgtc aataaaatcg agcgcgcata
     8101 ggagaacccg gtgccgcccg caatcaaaat catcggacgc tcttcatcat cgcgcagcca
     8161 cgcttctccg tagggaatat cgaccacgat ttgataatct ttgaggatgc ggtccatgac
     8221 tgctttcgcg taaaggttga tttcagaagc gccaatatgc agctctataa accctttttc
     8281 atccggcgtc gaagccattg aaaacggacg tttgtcgcgc tcatccatca ctaccatcaa
     8341 atactgacca gcacgaaaag aaaaggccgc gtctggcacg atgcggacac gatatacggt
     8401 atccgtgata gcttctaccg aggtcacttt acagcttaag gttgtcatgc gctttctctg
     8461 tcggatcgat aaatagggca aaacaaacgc gcatcaggcg cttttaccgt tgttaaaaat
     8521 agccagttca tcccagatgg cgtcaatatg cgcgacaaca tctggatctt ttttgatggg
     8581 acgtccccat tcacgctggg tttcccccgg ccatttattc gtggcatcca gccccatttt
     8641 tgaacccagc ccggagacag gcgaggcaaa atccagataa tcaataggcg tattttctac
     8701 cagaacagta tcccgcgccg ggtccatacg ggtggtaatc gcccaaatca catcgttcca
     8761 gtcgcgtgcg ttgacgtcat catcgcaaac gatcacaaat ttagtgtaca taaactggcg
     8821 taagaacgac cagacgccca tcatgacgcg cttcgcgtgt ccggcgtact gttttttgat
     8881 cgtcactacc gccagacgat aagaacagcc ttccggcggc aggtaaaaat cgacaatttc
     8941 cgggaactgt ttttgcagaa tcggtacaaa cacttcgttc aacgccacgc ccagtaccgc
     9001 gggctcatct ggcggacgcc cggtataggt ggaatggtaa atcgcatctt cacgctgggt
     9061 aatatgcgtc acggtaaata ccgggaaact atcgacttcg ttatagtaac cggtgtggtc
     9121 gccatacggc ccttccggcg cagtttcgcc ttgttcgata tacccttcca gcacaatctc
     9181 cgcactggcg ggcacttcaa ggtcattgga gatacacttc accacttcgg tcttggtacc
     9241 gcgcagcaat ccggcaaacg catactctga aagtgtatcc ggaacggggg tgactgcacc
     9301 gagaatcgtg gcgggatcgg cacccagcgc cacagaaacc gggaaacgtt cgcccggatg
     9361 cgccgcacac cactcctgat aatccagcgc gccgccgcga tgcgacagcc agcgcataat
     9421 cagtttgttt ttaccaatca gctgctggcg ataaatgccc agattctgcc gctctttatg
     9481 cgggccgcgc gtcactgtca gcccccaggt aatcagcggt gcggcatctt ccggccagca
     9541 ggtcataatg ggaatgcgat tgagatcgac gtcatcgcca gagacgattt tttgttggca
     9601 gggcacacca cgcagccgct ttgtcggcat gttcaatact tgcttaaact gcggcagttt
     9661 atcaaacagg tcgcggaaac cttttggcgg ctccggctct ttcagaaacg ccaataattt
     9721 accaacttca cgcagcgccg aaacatcttc ctgtcccatg cccatcgcca cgcgctttgg
     9781 cgtaccgaac aggttgcaca gcaccggcat tgagtagcct ttagggtttt cgaacaacag
     9841 cgcaggccca ccggcacgca aagtgcggtc agcaatttca gtgatttcca gatgcggatc
     9901 caccgggagc gtgatacgtt ttagctcacc ctgctgttca agcagcgtca agaagtcgcg
     9961 taaatcgtta tatttcatgg cgtccattgt agcctcttaa tctacgccca ttatacggcg
    10021 ttcatctttg cgatgctgta aatttgttaa attagcgtga actctgacgg tataacgcaa
    10081 accggggaat ataattaact tagcgtaaag cttttgctat ccttgcgccc cgattaaacg
    10141 gataagagtc attatgcaat cctggtattt actgtactgc aagcgcgggc aacttcaacg
    10201 tgcccaggaa cacctcgaaa gacaggctgt gaattgcctg gcaccgatga tcaccctgga
    10261 aaaaatcgtg cgtggaaaac gtactgcagt cagtgagcca ttgtttccca actacctgtt
    10321 tgtcgaattt gatccagaag tgattcatac cacgactatc aacgcgaccc gcggtgtcag
    10381 ccacttcgtg cgctttggcg cgtcgccagc gatagtccca tcggcggtga ttcatcagct
    10441 atcggtatat aaaccgaaag acattgtcga tccggcaaca ccttatccgg gtgataaggt
    10501 gattattacc gaaggcgcgt tcgaaggctt tcaggccatt ttcaccgaac ccgatggtga
    10561 ggctcgttcc atgctattgc ttaatcttat taataaagag attaagcaca gtgtgaagaa
    10621 caccgagttc cgcaaactct aaaacgcaat cccaaacagt gttttgacat tggcatccgt
    10681 ggtggcagcc agccatgcgg catcttctcc acgccagtgc gcaatacgtt gcaaaatatg
    10741 gtgcagatgg gctggctcgt tgcgccggga tgatggcttt ggcgtgaaat cgcgcgggag
    10801 cagatacggc gcatcagttt cgatcagcaa tttctccgcc ggaatcaacg gcaacaattc
    10861 ccgcagctcc agcccgcgtc gttcatcgca aacccaaccg gtaatgccga tataaattcc
    10921 acgcgccacg cacgcctgca tctcttcgcg tgtgccggta aagcaatgaa gaaccgcacc
    10981 aggcagttta tccagccacg gctccagcaa tgtcataaac cgctcgtggg catcgcgaca
    11041 gtgcataaat accggcatgt ttaattctgc ggcaatgcgt agctgagcaa caaaagcgcg
    11101 ttcctgctct tccggcgtcg aaaagttgcg gttaaagtcg agaccacatt caccaatcgc
    11161 caccacttct ggctgcgcgg ccagctcaat aatcgcttct tcagtcgcag cttgccactg
    11221 gctgctgtcg tgaggatgta cgcccgccgt tgaccaacag gacgaatact gacgcgccag
    11281 cttttgcgcc tgctggcttt cacgcaggtt agtgccggtg atgagtagcc cattaactcc
    11341 cgcgtcaaaa gcgcgcgcta caacatcatc acggtctttc gcaaattgcg aactggtcaa
    11401 attaacgccg atatcaaaca tcctgtactc catatgacaa ccgccctgac gggcggttga
    11461 atttattctt cagttttttc gctttctgct tcagcgtcgt tttcctcttc ccgatttcgc
    11521 cctttaccaa cgtaaaagcg tgagaagaag acaccgattt caaacagaca gtacatcggg
    11581 atcgccaata gcgtttgcga gaagacatcc ggcggcgtca gcaacatccc gacaacgaat
    11641 gcaccaacca gcacatacgg gcgttttttg cgtaagtctt ctggcgaggt aatccccatc
    11701 cagcacagca gcacaattgc caccggcact tcaaaggaga caccaaacgc cataaacagc
    11761 gccataacga agcttaaata gctggcgatg tcggtggata cctgcacccc ttccggcgcg
    11821 gtattggcaa ggaagccaaa tgccagcgga aagaccacaa agtaggcgaa tgccatgccg
    11881 atataaaaca gcagagagct ggaaaccagc agcggcacca ccaggcgacg ttcatgctta
    11941 tacagcgctg gggcgataaa tgcccacacc tgatagagaa tcaccggcgc tgacagaatc
    12001 agcgacacca taaaggtcag cttgatcggc gtaaagaacg gcgaggccac gtcggtggcg
    12061 atcatcgttg aaccttgcgg caactgcttg atcaatggcg cggataccag gtgatagatg
    12121 tcattggcga aatagaccag acacaggaat atcacgatca ccgcgataat gcagttcagc
    12181 agacgcttac gcagctcaat tagatgcgtg ataagcggtt gagtatcttc tacagacatg
    12241 tttacggttt atcactcgac gaaggggaag gtgcagcggt tttcggttca gcgtccgcag
    12301 caggttttac caccggctct ggcgtggttt ctggcttctg ttccggcgaa ctggcctgcg
    12361 tttgtgcagc ggcaggcgtt acgccctcat gcgcagcttc attatctttc accaccgggt
    12421 tatggatggt gtgcgcttca tcgctcgcct tttctggatc gtttgcaacg taggaacgct
    12481 tcatcgactc cgcggcctgg cgtagttcat ccatcgacgc tttcagttcg ggcgtcaggt
    12541 tagtgaggct cgccttttca acctttttca gactgtcctg aaactcctgg agttttaact
    12601 cctggatcag ttcgttctgc accgttgtcg ccagtgaacg caacgcgcga atccagcccg
    12661 ctaccgtttt taccgccaca ggcagtcgtt gcggccccag aacgacgagg ccgatgatga
    12721 acaccaatag cagttcgcta aaaccgatat caaacacgga ttacacctgc tctttatcgt
    12781 ggcgcttcgc gtcttctgtt ttagcctgtt cctgattcgt atccgcctgc ttatcggcga
    12841 tagttttcgc agtaaaatca gcatcctgac tggttttatc ctgctttggt tcatcatcgc
    12901 tcattgcttt tttaaagcct ttgatcgacg caccaagatc ggaaccgatg gagccgagct
    12961 ttttggtgcc aaaaagcagt acaacgatga cggcaataat caataactgc caaatactga
    13021 taccacccat acatgttcct ctgtggtaga tgatgattaa acaaagccgc attatacgtt
    13081 acacggcccg ccttgagcga tgaaaaaatc agcgtgtttt gcgccaaccg acaaaccagg
    13141 cgatcagacc acctgccatt aaccagccgg gcatcagccc ccactcaggt cggctgacca
    13201 acaagaatgt gccacttaat actaacgtag cgccaattcc gagaaaataa cgcgattgtc
    13261 cctgacgtac atgatttgac tgaagctcgc gggcaatctt atcaacactg tgctgtaaat
    13321 acttgccctg gcgcaaactg tcgtaaacca attcaggcag ttctggcatt ttttcgaccc
    13381 agaacggcgc tttttcttta aatgctctca ccagcgcagg aataccgacc tgatctttaa
    13441 tccacgactc caggaaaggc ttcgctgttt tccataaatc gagctgcgga taaagctggc
    13501 gtcctacccc ttcgacatag agcagggttt tctggagtaa caccagttgc ggctgcactt
    13561 ccatattgaa gcgacgcgcc gtattaaaca gatttaacag tacatgtcca aacgaaattt
    13621 cggccagcgg tttctcaaag ataggttcac agaccgtacg aatggcaaat tcgaactctt
    13681 caacgttggt atctggtggc acccagcctg aatcgacgtg tagctctgcc actttgcgat
    13741 agtcgcgatt aaagaaggcg ataaagtttt ctgccagata gcgtttatct tctttgttta
    13801 gcgagccaac aatcccgcaa tcaatgccga tatatttcgg gttttccggg tgttcatagc
    13861 ttacgaagat gttgccaggg tgcatatcgg catggaaaaa gctgtcgcga aagacctgag
    13921 tgaagaacac ctgcacgccg cgttccgcca gcaatttcat gttagtgccg tttttctcca
    13981 gcgccgcaac atcagacacc ggaatgccgt aaatgcgctc catcaccatc atcccttcac
    14041 tacaatagtc agggtaaact tccgggatgt agagcatcgg gctgtcttca aaattgcgcc
    14101 gaagctgaat ggcgttggca gattcccgca gcaaattcag ttcatcaatc aaggtctttt
    14161 cgtactcgcg caccacttcg gttgggcgca gacggcgacc atccggcagc aaacgcggca
    14221 cccagcgagc cagacggtag ataagtttca gatccgcttt aatgaccggc aaaatatccg
    14281 ggcggatgac tttaatcacc acctctttac cattcgattt caatcgcgcg gtatgaacct
    14341 gggcgataga agcagaagcc agcggcttga tttcaaaatc gtcaaaccac gcttctaccg
    14401 gcaagccgcc cattgcagct tcaatctgct gcttcgccag cttgccatca aacggagcta
    14461 ctttgtcctg caataacgcc agctgatcgg caatatgcgg tggaaaaaga tcgcggcggg
    14521 ttgataacat ttgcccgaac ttgatccaaa ccggccccag ttcttgcagg gccagtcgta
    14581 gtcgctcacc taaaagtttg tctttatgcc gatttggcat ccagaataat gagtatcgcc
    14641 atagccgtag cggcagggtg atacgcattt tggggatcag ttcatcaagt ccgtagctta
    14701 aaaaagtgcg aatgatgaaa tataggcgcc gtacttcacc tggcgtcatt tagcctccag
    14761 tttttccagc cgtttggtca gggcatcaac agcacgctcg acggcagccg tctcttccgc
    14821 aaaccaggcc acttcaagcg gaccgggtgc catacgccac tcttcagtaa tggcttccgc
    14881 cacataacgt tgctggcgct taatgccgtg atgcaggaac tttgcgcctc cgcgcatggc
    14941 tttgctgatt ccttcagcgg cgatatcacc ggtataaggg gccagcagtt ccgcagggtc
    15001 gaactctgcc agatctgcca gcgcaacgaa gttttgcacc acctgaatat cgccctgcac
    15061 ttccagctca ccactgcgaa tcagtgcggt aagctgctgg cgatcgcgaa gtttcggcaa
    15121 cacactggcg taggcgataa cggtgcagtc agcatcgcct gcccattcgc ccagtacatc
    15181 aacctggcgt tcgctgaaca ccagaatcaa tgacgtcgaa aagcctttta cctccacgcg
    15241 caatacttta cccagcagac gcgagcgggc tgttttcagc gcgggtgagc gatacaggaa
    15301 ggtgttgagc agactttcaa ttcctgccgt cactaaaggt ttaaaaggca tttccggtct
    15361 cctgtcagaa cttataacca cgatgcagcg ccacaacccc tgccgtcaga ttgtagtagt
    15421 cgacactttc gaatccggca tcctgcatca tggctttcag ggtatcctga tcgggatgca
    15481 tacggatgga ttctgccaga taacggtagc tgtcggcgtc gttcgcgacc agtgagccaa
    15541 tacgcggcag cacatggaag gagtatgcat cataggcttt gctcagcggc tcgataattg
    15601 gcttcgagaa ctcaagcacc agcaggcggc cgccgggttt cagcacgcga tacattgaac
    15661 gcagtgcttt atctttgtcg gtgacgttac gcagaccaaa cgaaatggtg atgcaatcaa
    15721 aggtgttatc cgggaacggc agcgcctcag cgttcgcctg aacatactca acgttgccaa
    15781 tcacaccgat attacgcagc ttctcgcgtc ccattttgag catggattca ttgatatcag
    15841 caaggaccac tttgccagtt tctccgacca ggcgggagaa tttcgctgtc aggtcgccgg
    15901 tgccaccagc cagatccagc acggtctgcc cacggcgtac gccgctgcaa tcaatcgtga
    15961 atcgcttcca caaacgatga ataccaaatg acatcaaatc attcatgaca tcgtattttg
    16021 atgccacgga atggaaaacg tgggcgacca tatccgcttt ttgttccttc gcgacggtct
    16081 gaaaaccaaa gtgcgtcgtt tcttgtgact tatccaccat ctcaatgcct gctcatcaaa
    16141 aaattgttcc agaagtgtaa cagattgggc gtcgatgccc tagatttcta cccggcttaa
    16201 ctacccctaa tgggctagcg cgactgctga ttatattcat catcgcgttg ataagcttca
    16261 tcattcggct gctccggaac cgaccgaagt cgatactctt catcctggct caccgcctgt
    16321 tcagccaaat ccggattaat ctcgcgttta atttctactc ctaaaccgcg aaacgcttct
    16381 gcctgcgcca gcacatttcc gcgacctgaa gagagttttt tcattgcctg gcggtagtta
    16441 tcctgcgctt tgtcgagact ttgaccaatc gcggacatgt catcgatgaa caaacgcatc
    16501 ttgtcgtaca gcttgctggc acgatcggcg atttgctggg cgttgcggct ttgatgctca
    16561 taacgccaca ggttggcaat agtacgcaac gccaccagca gcgtagtcgg gctaaccagc
    16621 atgatattgt ttttcaacgc ttcggtgatc agctccggct ggcggtcaag cgccagtaaa
    16681 aaagcgggtt caacgggaat aaacatcagc acgtaatcca gagttcgcag ccccggcagc
    16741 tgttgataat ctttgcgtcc cagcaaacgg atatggttac gcaccgacgc gatatgttcc
    16801 tgtagcgcgc tttcgcgggt gtagtcgtct tcggcgttaa aatagcgttc ataggcgacc
    16861 agcgtcattt tggcgtcgat caccacatct tttccctgcg gcagccgcac gatgacatcc
    16921 ggctgcatcc gcgagcgggc gtcattttcg atgctgacct gggtttcata ttcataccct
    16981 tcacgcagac cggaagcctc cagcacccgc gtcaatacta cctcgcccca gttgccctgg
    17041 gttttattgt cgcctttcag cgcgcgcgtc aggttgatcg cttcctgggc catttgcgcg
    17101 ttgagttgct ggagattgcg aatttcgtgg gtcagggtat ggcgttcttg tgcttcttta
    17161 ccgaagctgt cctgaacctg acggcggaaa ccgtccagtt gttcacgtag cggcgacaac
    17221 aggctgttca gactctgacg gttttgctca tcaacccggc gattgctgtg ctcaaaaata
    17281 cggttggcga ggttttcaaa ctgctcactg aggcgctgct cgctgttaat catctggcga
    17341 attttatcgt cagcatgttg ctgtgcggct tccatccgcg tggttacttc acgcagatcg
    17401 gcctccagag aggtgttaat actttgcagg ctgcgcactt cgttattgag taactcgcac
    17461 tctgcacgcc agtgctcgct ttgggtaatt tgttgttttg ccgcgcttaa ctccgcgacc
    17521 atctcttcac gttcagctaa ttgctcggct ttttgctgcg catgttgata actggcaaac
    17581 agccagccaa ttgccacacc caccaacgca ataactgcgt aaaccatgat tgagaaatcc
    17641 acaacgcctc ctgacgcaac aacttacccg cacaaaatag aattgtgctg tacaaacgtc
    17701 cagttggaaa ggcatttcct gcgaggcgct acgcaaaaat acaaaaggcc gaacgcgtcg
    17761 gccttcagac aggagaagag aattacagca gacgacgcgc cgcttccacc acgattttca
    17821 ccgcttggct ttcagtttgt ttcatcgtct cagcattcgg gatctcttgc tgggtgcggt
    17881 taacgataac acccgctacc ataccggcac gcaggccctg acttgcacac atggtcagca
    17941 gggttgcaga ttccatttca tagttcatta cgcccatcgc ctgccactct tccatagaac
    18001 ctttaaagtg acgaactacg cgaccagagt aagtatcgta acgttcctgg cctgggtaga
    18061 aggtatcaga agaagctgtc acgccaacgt gagttgtcgc gccaatggat ttcgcagctt
    18121 caaccagcgc agtcgtacat tcgaaatcag cgacagccgg gaattccagc ggtgcgaagt
    18181 gcaggctcgc gccatccaga cggacagacg ccgtggtaac caggacatca cccacattaa
    18241 tatgcggctg aatagcgccc gttgtaccga tacgcaggaa ggtgcgaatg cccagctgtg
    18301 ccagctcttc aacagcaata gaggtagacg ggccgccgat accggtagag cagacgataa
    18361 caggtttacc atccagctct gcacgccagg tggtgaattc gcggtgagat gccagcttaa
    18421 ccggcttatc catcagcgcg gcgatctttt ccacacgatc cgggtcgcca gggacgatgg
    18481 caagcgtagc cccttgtaaa tcgtttttag tgaggccgag atgaaaaaca tcagacttgg
    18541 acatatacaa ctcctctgtg aatcggttta gtcagaagaa gcaaaaagac actttaccga
    18601 agggtttaac attttttcgt gatactcatc accatgacgc aaatgcgttg cataaattta
    18661 cccaccaaaa gtgatgcaaa tcacataaat cactcttcat tctgcaggac tgcataaaag
    18721 atgagccgat tcaggcaatg tgatttgttg catgctgact atagttaaca ttgcgcaaca
    18781 tttttataag ggtacggaga ataccatggc aacaacacaa caatctggat ttgcacctgc
    18841 tgcatcgcct ctcgcttcga ccatcgttca gaccccggac gacgcgattg tggcgggctt
    18901 cacctctatc ccttcacaag gggataacat gcctgcttac catgccagac caaagcaaag
    18961 cgatggccca ctgccagtgg tcattgtagt gcaggaaatt tttggcatgc atgaacatat
    19021 ccgcgacatt tgtcgccgtc tggcgctgga ggggtatctg gctatcgcac ctgaacttta
    19081 cttccgcgaa ggcgatccga atgattttgc cgatatcccc acgttgctta gcggtctggt
    19141 agcaaaagtg cctgactcgc aggtgctggc cgatctcgat catgtcgcca gttgggcttc
    19201 ccgcaacggc ggcgatgttc atcgtttaat gatcaccgga ttctgctggg gtggacgtat
    19261 cacctggctg tatgccgcgc ataatccaca gctaaaagcc gcagtggcgt ggtacggcaa
    19321 actgacgggc gacaagtcgc taaattcacc gaaacaacct gttgatatcg caaccgatct
    19381 taacgcgccg gttctcggct tatatggtgg tcaggataac agcattccgc aggagagcgt
    19441 tgaaaccatg cgccaggcgc tgcgggctgc taatgcgaaa gcagagatta tcgtgtaccc
    19501 ggacgccggg catgcattca acgctgatta tcgcccgagc tatcatgccg aatctgcgaa
    19561 agatggctgg cagcgtatgc tggaatggtt taagcagtat ggtgggaagc ggagttagtt
    19621 tcctcttagt ctatatttga gatggtgagt gaacgagtgc gttttgctgc catctctgtc
    19681 ccccaaaaaa taatcagtat gaattgattg gagattacga ctccgctatt tgaccttaag
    19741 aaagtcggca agtcatagtc ataaatgagt ctcttcaatg gaagacacct tagagattta
    19801 ccatctactc ccattcacaa acaaacatta attattatca acacattcac attaattata
    19861 aatcatctca gaccttcctg ataaaaagat attaaagtga aacattttgt ttttcacatt
    19921 ttagttcatc acttttaggc gtaaataaag gtaaaaaaaa caacaatact atttctcttt
    19981 cacaaaagaa ttaacaaaag gttttttaag gatgagtgat attatttact tgaccataac
    20041 cggagaacaa caaggatgta tttcctctct ttgtggaacg agcgcatcaa taggtaatcg
    20101 ctggcagatt ggccatgaag atgaaatctt cgcgttctcg ctctctaata gtattacgaa
    20161 tgcaggcaaa gggtcacaat tgcatggcct tagtttctgt aaattcttat taacgctatt
    20221 aacaataacg agcagttgtt catggcgttt gacttctatc ggatcaatag atttggtcga
    20281 tgggagaaat attacaacat tcaactccgc ggcgcgctct tatcagcaat caatcacctg
    20341 tttactgaga acaatctgga tacagaagcc atcactgtca gttatgaata tatactctcc
    20401 aggcatctga ttgctaatac tgaatttagt tatttggcat ttcccgataa ctacaaccgt
    20461 ctgtttatac cacggccaaa aactaaagct ggtaatggtt caaaacactt aatagtaaag
    20521 cggttggcag actgcttgct gctggcggca tatacaatgg gaatatcgaa ggattcaggg
    20581 aaacagcaga caagttgggg ggagatgcac ctgctggtta cgatcaattt atggataaca
    20641 aagggttact catcgctggt gcttcaatag ttgctggagc agttctgggg agaatgagac
    20701 tccctgattt ggaatcactt gagcatttcg gagccagagg agcagtatca ggaag
//
LOCUS       DAJVOY010000060        20745 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVOY010000060 DAJVOY010000000
VERSION     DAJVOY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.060"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..271)
                     /locus_tag="OQL90_001949"
     CDS             complement(<1..271)
                     /locus_tag="OQL90_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368740.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAILRCTGNSGHYHLFFF"
     gene            complement(524..994)
                     /gene="bcp"
                     /locus_tag="OQL90_001950"
     CDS             complement(524..994)
                     /gene="bcp"
                     /locus_tag="OQL90_001950"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1368741.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(994..1566)
                     /locus_tag="OQL90_001951"
     CDS             complement(994..1566)
                     /locus_tag="OQL90_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1368742.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1712..2590
                     /gene="dapA"
                     /locus_tag="OQL90_001952"
     CDS             1712..2590
                     /gene="dapA"
                     /locus_tag="OQL90_001952"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1368743.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2607..3641
                     /gene="bamC"
                     /locus_tag="OQL90_001953"
     CDS             2607..3641
                     /gene="bamC"
                     /locus_tag="OQL90_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1368744.1"
                     /translation="MAYSVQKSRQAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3854..4567
                     /gene="purC"
                     /locus_tag="OQL90_001954"
     CDS             3854..4567
                     /gene="purC"
                     /locus_tag="OQL90_001954"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1368745.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4735..5598
                     /locus_tag="OQL90_001955"
     CDS             4735..5598
                     /locus_tag="OQL90_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1368746.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5613..7628
                     /gene="tmcA"
                     /locus_tag="OQL90_001956"
     CDS             5613..7628
                     /gene="tmcA"
                     /locus_tag="OQL90_001956"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1368747.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7702..8400
                     /gene="ypfH"
                     /locus_tag="OQL90_001957"
     CDS             7702..8400
                     /gene="ypfH"
                     /locus_tag="OQL90_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1368748.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8510..8710)
                     /locus_tag="OQL90_001958"
     CDS             complement(8510..8710)
                     /locus_tag="OQL90_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1368749.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8738..9865)
                     /gene="dapE"
                     /locus_tag="OQL90_001959"
     CDS             complement(8738..9865)
                     /gene="dapE"
                     /locus_tag="OQL90_001959"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1368750.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9869..10225)
                     /locus_tag="OQL90_001960"
     CDS             complement(9869..10225)
                     /locus_tag="OQL90_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1368751.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10606..10665
                     /gene="ypfM"
                     /locus_tag="OQL90_001961"
     CDS             10606..10665
                     /gene="ypfM"
                     /locus_tag="OQL90_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1368752.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(10764..13876)
                     /gene="acrD"
                     /locus_tag="OQL90_001962"
                     /pseudo
     CDS             complement(10764..13876)
                     /gene="acrD"
                     /locus_tag="OQL90_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(14039..15739)
                     /gene="narQ"
                     /locus_tag="OQL90_001963"
     CDS             complement(14039..15739)
                     /gene="narQ"
                     /locus_tag="OQL90_001963"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCS1368753.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            15945..17924
                     /gene="aegA"
                     /locus_tag="OQL90_001964"
                     /pseudo
     CDS             15945..17924
                     /gene="aegA"
                     /locus_tag="OQL90_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            17992..18567
                     /gene="nudK"
                     /locus_tag="OQL90_001965"
     CDS             17992..18567
                     /gene="nudK"
                     /locus_tag="OQL90_001965"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1368754.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            18693..19736
                     /locus_tag="OQL90_001966"
     CDS             18693..19736
                     /locus_tag="OQL90_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1368755.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(19846..20745)
                     /locus_tag="OQL90_001967"
                     /pseudo
     CDS             complement(19846..20745)
                     /locus_tag="OQL90_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         5020 a         5303 c         5550 g         4872 t
ORIGIN      
        1 aaaaaaagaa caaatggtag tgtccgctat tgccagtaca cctcaggatc gcatcatccc
       61 tctgggagat ggcattgacc gggcagacct gcaggcaagg ggcttcctca cagtgatgac
      121 acacgacagg ggcagtgatt gttgacgtct tcgccagggc caggcgcggg tgttgctgta
      181 aaccttgcgt tttatgcacg tccgaacagg cagcgagaca ggtattacat cctgtacacc
      241 acagtggttc ggccaccaca aagcggttca tgtcggctcc ttgaaaatga tatgaaacat
      301 tgcatttcat attgcataaa ggatgccagt ctgattaatt taatatattc aataaattat
      361 gtattaacag ggttctgaac ggtacaaaaa tgatggtcat taacgttcag caaaaaatgg
      421 attcgacacc cgtgtcgcag gggaaagcga gaatgtgatc ttgatcacga gaagtgaggt
      481 gtgctgaccg caagcgcagc cagcacggaa tggagcaaag taatcaggcg tgttctttca
      541 gccagttcag cacaacgtcg tggtgattgc tggttttaaa atcgtcaaag acatgttcga
      601 ttttgccatc agcgtcaatc aggaagctga tgcgatgaat accatcgtag gttttgccca
      661 tgaaggactt ttcaccccag acgccgaatt gttcgcacac ctggtggtcc tcatcagaca
      721 ggagcgtaaa gttaagcagc tctttttccg caaaacggga gagtttttcg ggtttatcgg
      781 tgctgatacc cagcacatca acgcccgctt ttttcaactc atccatgtta tcgcgtaagc
      841 cgcaggcctg tacggtacag ccgggggtca tggctttcgg gtagaaataa accagaacac
      901 gctgtccctg gaagtcggtc aaattaactt gttctccgtc ttgatccggc aagctaaatt
      961 tcggtgcgat atcaccggct ttcagtggat tcattactta actccatcct gttcatcatg
     1021 ttgggaataa ttgacgacgt taatactgcc ttgtgcattg agttctgtac atagggcttt
     1081 gaacgcttgc tcaatatttg ctgcgtccgc agatgcgggg ctgtgggcgg tgatctgaat
     1141 atgcaactgc gcagcccgtt cattttcagc aggttgcgtg cgcgacacca gctccgcaat
     1201 gttcatatga tgcgcgtcga aaagtgctgt gaagcgttca attaaatgcg gggagtctgc
     1261 aacatcgacc tgaacccaga cagatgctgg cattggcgga cgcggacgcg ccgtcgtgcg
     1321 cttcatcacg attaaaagat ccagttcggc acctttcaac ggtaacgttg attcaatcag
     1381 agtaatggca ttccatgaac cggaaagcag catgataaac gtgaactctt ctcccagcat
     1441 cgccaggcga ctgtcttcaa tattacagcc gcaactactg acatgacggg tgatggtgtt
     1501 cacaattcca gggcgatcgg cacccaacgc agtgatcacc agataatgtt gcgatgacag
     1561 tgtcaaactg gttattcctt taaggggtga gttgttctta aggaaagcat aaaaaaaaca
     1621 tgcatacaac aatcagaacg gttctgtctg cttgctttta atgccatacc aaacgtacca
     1681 ttgagacact tgtttgcaca gaggatggcc catgttcacg ggaagtattg tcgcgattgt
     1741 tactccgatg gatgaaaaag gtaatgtctg tcgggctagc ttgaaaaaac tgattgatta
     1801 tcatgtcgcc agcggtactt cggcgatcgt ttctgttggc accactggcg agtccgctac
     1861 cttaaatcat gacgaacatg ctgatgtggt gatgatgacg ctggagctgg ctgacgggcg
     1921 cattccggtg attgccggga ccggtgctaa cgctactgcg gaagccatta gcctgacgca
     1981 gcgcttcaat gacagcggta tcgtcggctg cctgacggta accccttact acaatcgtcc
     2041 gtcgcaagaa ggtttgtatc agcatttcaa agccatcgct gaacatactg acctgccgca
     2101 aattctgtat aatgtgccgt cccgtactgg ctgcgatctg ctcccggaaa cggtgggccg
     2161 tctggcgaaa gtaaaaaata ttatcggaat caaagaggca acagggaact taacgcgtgt
     2221 aaaccagatc aaagagctgg tttcagatga ttttgttctg ctgagcggcg atgatgcgag
     2281 cgcgctggac ttcatgcaat taggcggtca tggggttatt tccgttacgg ctaacgtcgc
     2341 agcgcgtgat atggcccaga tgtgcaaact ggcagcagaa gggcattttg ccgaggcacg
     2401 cgttattaat cagcgtctga tgccattaca caacaaacta tttgtcgaac ccaatccaat
     2461 cccggtgaaa tgggcatgta aggaactggg tcttgtggcg accgatacgc tgcgcctgcc
     2521 aatgacacca atcaccgaca gtggtcgtga gacggtcaga gcggcgctta agcatgccgg
     2581 tttgctgtaa agtttaggga gatttgatgg cttactctgt tcaaaagtcg cgccaggcaa
     2641 aggttgcggg tgtttcgctt attttattac tcgctgcctg tagttctgac tcacgctata
     2701 agcgtcaggt cagtggtgat gaagcctacc tggaagcggc accgcttgcg gagcttcatg
     2761 ccccggctgg aatgattttg ccggtgacct ccggtgatta tgcaatcccg gtgaccaacg
     2821 gtagtggtgc tgtcggtaag gcgctggaca ttcgtccacc agcccagccg ctggcactgg
     2881 tttctggcgt gcgtacccag ttcacgggcg ataccgcttc attgctggtg gaaaatggtc
     2941 gtggcaatac tctgtggccg caggtggtta gcgtgctgca ggcgaaaaac tacaccatca
     3001 cccaacgtga tgatgctggt cagacactga ccatcgattg ggtacaatgg aaccgtctgg
     3061 acgaagacga gcagtatcgt ggtcgttatc aaatctctgt taagccgcag ggttatcagc
     3121 aggcggttac ggttaaactg ctgaacctgg aacaggcggg caaaccggtt gcagacgcgg
     3181 cttccatgca gcgttacagc acggagatga tgaacgttat ttccgccggt ctggataaat
     3241 ctgcctctga cgccgcgaac gctgcgcaaa atcgtgcctc caccactatg gacgtacaaa
     3301 gtgcagctga tgacaccggt ttaccgatgc tggtcgtacg cgggccgttc aatgtggttt
     3361 ggcaacgtct gccagcggcg ctggaaaaag tgggcatgaa agtgaccgac agcacccgtt
     3421 cgcagggtaa catggccgta acttataagc cgctgtctga cagcgactgg caggaactgg
     3481 gcgcgagcga tccaggcctg gcatccggtg actataaact gcaggttggc gatttagata
     3541 accgcagcag cctacagttc atcgatccga aaggtcatac tctgactcag agtcagaacg
     3601 acgcgctggt agctgtcttc caggctgcgt ttagcaagta aaaatacagg gctggagtca
     3661 tccggccctt ttttctgata tgatacgcaa acgtgtgcgt cggcagaaaa atgcaatttt
     3721 agcggtaatt cgcacgaaat ttgtttgtcg gacgtaggtc ggataaggcg ttcttgccgc
     3781 atccgacaaa acatccggca caccagacag caaaagattt taaaacgtta attcacaccc
     3841 aggagtgata aagatgcaaa agcaagctga gttgtatcgt ggtaaagcga aaaccgtata
     3901 cagcacggaa aacccggacc tgttggtgct cgaattccgc aatgatacgt cagcagggga
     3961 tggcgcgcgc attgagcagt ttgatcgcaa aggtatggtg aacaacaagt tcaactactt
     4021 cattatgagc aaactggctg aagcgggtat cccgactcaa atggagcgtc tgctctccga
     4081 taccgaatgt ctggtgaaaa agctggatat ggtgccggtt gagtgtgtcg tgcgtaaccg
     4141 tgctgctggc tctctggtga aacgtcttgg aatcgaagaa ggtattgagc tgaacccgcc
     4201 gctgttcgat ctgttcctga aaaacgatgc catgcacgat ccgatggtca acgaatctta
     4261 ctgcgaaacc tttggctggg tgagcaaaga gaacctggcg cgtatgaaag agctgaccta
     4321 caaagcgaac gacgtgctga aaaaacggtt cgatgatgct ggtctgattc tggtcgactt
     4381 caagctggag tttggtctgt acaaaggtga agtggtactg ggcgatgagt tctccccgga
     4441 cggcagccgc ctgtgggaca aagaaacact ggagaaaatg gacaaagacc gtttccgcca
     4501 gagcctcggt ggcctgatcg aagcctatga agccgtcgcc cgccgcctgg gtgtacagct
     4561 ggactgattt ttctgttcat catcttgccg tgcagctggc acggcaagac aaccgctctc
     4621 ggaatatatg cattgaacgc tcattcctcc gccatttcat cccgctttcc tgtggtaatc
     4681 ttcctctgaa ccacctacga tcatttctat aatgaatata ttgttgaggt atctatgcgt
     4741 tggcaagggc gacgtgaaag tgacaatgtt gaagacaggc gcaacagctc cggtggtccg
     4801 tctatgggcg gtcccggttt tcgcctgcca agcggtaaag gcgggctgat tttactgata
     4861 gtcgtgctgg ttgcaggcta ctatggtgtt gatttaaccg ggttaatgac tggacagccg
     4921 gtttcccaac aacaatcaac gcggtccatt agccctaatg aagacgaagc tgcaaaattc
     4981 acctcggtga ttctggcaac cacggaagac acctggggac aacagttcga gaagatgggt
     5041 aagacctatc agcaaccgaa gctggtcatg taccgtggaa tgacgcgcac cggctgcggg
     5101 gcgggccagt ccattatggg gccgttctat tgcccggcgg atggcacagt ttatatcgat
     5161 ctctccttct atgatgacat gaaagacaaa cttggcgcgg atggcgattt tgcccagggg
     5221 tacgttatcg cccatgaagt cggtcatcat gtgcagaaac tgttaggcat cgagccgaaa
     5281 gttcgtcaac tgcaacaaaa cgcgacgcag gcggaagtga accgcttatc tgtgcgtatg
     5341 gaactccagg ccgactgttt tgccggtgtc tgggggcata acatgcagca gcaaggcgtt
     5401 ctggaaaccg gcgatctgga agaggcgctg aatgcggcgc aggccatcgg cgatgaccgt
     5461 ttacaacagc aaagtcaggg gcgagtagta ccagacagtt tcacacatgg cacttctcag
     5521 caacgctaca gctggtttaa acgtggtttc gacagcggcg atccggcaca atgcaatact
     5581 tttggtaaaa gcatttaatt tccggggcag ggatggctga actgactgcg ctttacacat
     5641 taacagcgca aatgaaacgt gaagggatcc gccgcttgct ggtgttgagc ggggaagagg
     5701 gttggtgttt tgatcatgcg cttaagttgc gtgatgcctt acttggcgac tggctgtgga
     5761 tttcgccgca gccagatgct gaaaaccact gttctccctc ggcgctacaa actttacttg
     5821 ggcgcgagtt ccggcatgcg gtattcgacg cccgccacgg ctttgatgcc gctgcctttg
     5881 cggcacttag cggaacgttg aaagcgggaa gctggctggt tttgttactc cctgtatggg
     5941 aagagtggga aaaccaacct gatgccgact cgctgcgctg gagtgattgc cctgacccta
     6001 ttgcgacgcc gcattttgtc cagcatctca aacgcgtact tacggcggat aacgacgcta
     6061 ttctctggcg gcaaaaccag ccgttctcgt tggcgcattt tactccccgt actgactggc
     6121 accccgcgac cggcgcacca caaccagaac aacagcaact cttacagcag ctactgacca
     6181 tgccgccggg cgtggcagcg gtaacggctg cgcgtgggcg cggtaagtcg gcgctggcag
     6241 ggcaactcat ttctcgtatt gcgggcagtg cgattgtcac cgcgcccgca aaagcggcaa
     6301 cggatgtact ggcacaattt gcgggcgaga agtttcgctt tattgcgccg gatgccttgt
     6361 tagccagcga tgagcaagcc gactggctgg tggtcgatga agccgcagcc atacctgcgc
     6421 cgttgttgca tcaactggta tcgcgttttc ctcgaacgtt gttaactact acggtgcagg
     6481 gctacgaagg caccggacgt ggttttttgc tgaaattttg cgctcgcttt ccgcatttac
     6541 accgttttga actgcaacag ccgatccgct gggcacaggg atgcccgctg gaaaaaatgg
     6601 ttagtgaggc actggttttt gacgatgaaa acttcaccca tacaccacaa ggcaatatcg
     6661 tcatttccgc atttgaacaa acgttatggc gaagcgagcc agaaacgccg ttaaaggttt
     6721 atcagttatt gtctggtgcg cactaccgga cttcgccgct ggatttacgc cggatgatgg
     6781 atgcaccagg gcaatatttt ttacaggcgg ctggcgaaaa cgagattgcc ggagcgctgt
     6841 ggctggtgga tgagggggga ttatctcaac aactcagtca ggcggtatgg gcaggttttc
     6901 gtcgcccgcg gggtaatctg gtggcccagt cgctggcggc gcacggtagc aatccactgg
     6961 cagcgacatt gcgtggacgg cgggtcagcc ggatagcagt tcatccggct cgtcagcgcg
     7021 aaggcgttgg gcaacagctc attgccagcg ctttgcaata tacgcaagac ctcgactatc
     7081 tttcggtgag ttttggttac accggggagt tatggcgttt ctggcaacgc tgcggttttg
     7141 tgctggtacg aatgggtaat catcgtgaag ccagcagtgg ttgctatacg gcgatggcgc
     7201 tgttaccgat gagtgatgcg ggtaaacagc tggctgaacg tgagcattac cgtttacgtc
     7261 gcgatgcgca agctctcgcg cagtggaatg gcgaaacact tcctgttgat ccactaaacg
     7321 atatcgtgct ttctgacgac gactggcttg aactggccgg ttttgctttt gctcatcgtc
     7381 cgctattaac gtcgttaggt tgcttattgc gtctgctaca aaccagtgaa ctggcattac
     7441 cggcgctgcg tgggcgttta cagaaaaatg ccagtgatgc gcagttatgt accacactta
     7501 aacttgcagg ccgcaagatg ttactggtcc gtcagcggga agaggccgcg catgcgctgt
     7561 tcgcacttaa tgatgttcgc actgagcgtc tgcgcgatcg cataacgcaa tggcaatttt
     7621 ttcactgact ccttcaattt tctcccatgg tcattgtgag ccagcggcgt aaaattaatg
     7681 acatcagtta aggagttcgc catgaaacat gaccattttg ttgttcaaag cccggataaa
     7741 cctgtgcaac agttgttact gcttttcctc ggtgtcgggg ataactcggt ggcaatgggg
     7801 gaaataggca gttggtttgc accgctgttt cctgatgcgc tggtggtgag tgtcggtggc
     7861 gcggagccga gcggtaatcc ggcggggcgt cagtggtttt cggtgcaggg tattacggaa
     7921 gataatcgcc aggcgcgtgt ggatgccatc atgccgacgt ttattgaaac ggtacgctac
     7981 tggcagaaac agagtggagt aggggctaac gccacggcgt taatcggttt ctctcaaggc
     8041 gcgattatgg cgctggagag tattaaagcc gaaccgggcc ttgcttcacg cgtcattgct
     8101 tttaacggac gctatgcgag cctgccggaa accgcgtcta cagcaacgac aattcacctg
     8161 attcatggcg gtgaagatcc ggttatcgat ctggcgcatg cggttgcggc gcaggaagcg
     8221 ttaatcagtg ccggtggtga cgtgacgctg gatatcgtgg aggatttagg ccatgcgatc
     8281 gacaatcgca gtatgcaatt tgccctggat cacttgcgtt ataccattcc gaaacattat
     8341 ttcgacgaag cgctaagtgg cggtaagcct ggcgatgatg atgtgattga aatgatgtaa
     8401 aagacagatt acctggcctg ataagacgcg tgagcgtcgc atcaggcatc gttcaccatt
     8461 gccggatgcg gcgttaacgc cttatccggc ctacggttga gcactcagct tacttcttcg
     8521 gttgatcctt tttcggccag tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg
     8581 ggggaagctc cggcttgttg gcgagaaatt tcttgtggtc gacgcgcttg agatctttaa
     8641 tcacgttcag caggacgcct accaaaaaga caatcaccag aatccaccaa tatttagcca
     8701 gccagtccat gcttatttcc tcttgcagaa ccactcatca ggcgacgagc tgttccatga
     8761 tacgttgata catacgggca agtagctgca ggtcggcagc gttcacacat tcattaattt
     8821 tatgaatagt ggcattgacc ggcccgagtt ccaccacctg cgcccccatg cgagcaataa
     8881 agcgcccgtc ggacgttccg cctgtggtca gtagctgcgg tttaatttca ttatagtgct
     8941 caaccgcgtt aacgaccgca tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg
     9001 aaagccacca atccaccgta tagcgcagtt gatgtttttc aagcagggca agcacctgcg
     9061 ctttgatcat ctcatcagtc agttcggtgc tgaagcggaa gttaaactgc acaaacagtt
     9121 cacccggaat aacattgtta ctgcccgttc ccgcctgaat attagcaatc tgcatactgg
     9181 tcgccgggaa aaattcattg ccctgatccc actcaatagc cactaattca ttaaggaaag
     9241 gtgctgcgcg atgtactgga ttgtcagcca gatgcgggta ggccacatgc ccctgaacgc
     9301 catgaatggt aaggttgcag gttaacgatc cgcgacgacc atttttcacc acatcaccaa
     9361 ccacttcgat actcgacggt tcgccgacca ggcagtaatc gagacgctca ttacgtgcca
     9421 tcaacgcttc gacgactttt accgtaccgt tgtgggcact ggcttcttca tcagaggtga
     9481 tcagaaatgc cagtcgcccc gtatggttgg gatgttgtgc gacaaagcgt tctgccgcta
     9541 ccaccatcgc cgccagcgag cctttcatat ctgccgcacc gcgcccgaat aacatgccat
     9601 cacgaatggt gggttcaaac ggcggattga tccaacgatc ggcgtcgcca ggcggcacca
     9661 cgtcggtatg cccggcaaag gctaacgttt caccttgccc acgccatgcc caaaaattct
     9721 gcgtatcggc aaagtccatg cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca
     9781 acaaagcctg gcatcctgcg tcatcaggac tcagggaagg gcggcgaata agctgttgtg
     9841 tcagctcaat aaccgggcac gacatagact acacctcatg gaaaaattgc tgataactgg
     9901 aatcactgaa acccagcagc ataggcttac caggcgcgca gagcaatgga cgtttgataa
     9961 ttgaaggcat ttcagtcatt aatgccgccg cagaggccgc atcggtgatt ttattgcggg
    10021 tggtttcgtc cagtttacgc caggttgtac cacgggtgtt cagtaacgct tcccagccta
    10081 attcgttgat aaaaccgttc aataattcgc tgtccagccc atcgacgcgg taatcatgaa
    10141 aacgatagtc gatgttattg gcttccagcc aacggcgagc ctttttaatg gtgtcacaat
    10201 ttttgatgcc gtaaagtgta accataatga atcctttttc gatctatgag aataaatact
    10261 catttaaggg tgaaaacatt ttgcctgcaa atgattatca ggaaatataa cattatcgaa
    10321 gtattcaaat taacaactat taaataatat taaacatttt taacgtaaac attaagaata
    10381 ttcatcttgt tatctgtctg tattttaacc agaattaaat cagtattaga tgccgtatga
    10441 atatctcgaa atgttgctgt gtatcacata caaattatca ccgcaggacc atagtattta
    10501 cataaggttt gtcccccgga ggtagtcatc gcagcaatcg agtaaattag cttcaccacg
    10561 gtatattgtt tccgtagaat acccataatt ataagagagg ttgttatgat tgaacgtgaa
    10621 ctggggaact ggaaagactt tatcgaagtt atgcttcgta agtaattcct gagacaaagt
    10681 acaaacagca agaacccgct gagcaggttc ttaatcggaa agttgaaaaa ggcgacacat
    10741 tagcatgtcg ccttttttat tgcttattcc gggcgcggct tcagcgggaa gcggcggcgc
    10801 accagtacaa agaacagcgg cacgaagtaa atagccagaa tagtggccga aatcatcccg
    10861 cccattacgc cagtacccac cgcatgctga ccaccggaac cggcacccgt gctggttgcc
    10921 attggcaata cgccgaagat aaatgccagc gaggtcatca gaatcgggcg taaacgctga
    10981 cggcaggcgt ggagcgtcgc ttcaaacagg tcgtggcctt tttggttcat ctcgttagca
    11041 aactcgacga tcaggatggc gtttttcgcc gataaaccaa tgaccgttaa caggcccacc
    11101 tggaagtaaa cgtcgttttc cagcccgcgc atccaggttg ccagcagcgc gccgataacc
    11161 cccagcggca cgaccagcat taccgagaac ggcaccgacc agctttcata caacgccgcc
    11221 agacacagga ataccaccag caaggaaatg gcgtacagcg ccggagcctg cgcgccggaa
    11281 agccgctcct gatacgacat cgccgtccac tccagaccaa agccgtttgg caactgcttc
    11341 actaacgatt ccattatatc catcgccgta ccggtactga cccccggtgc ggcttcccca
    11401 acaatctcaa ccgcagaata accgttatag cgttccagac gcggcgagcc agtctcccag
    11461 cgtgaggtcg cgaaagcaga gaagggcacc atgccgccat ctttatttcg gacataccag
    11521 agattaatgt catctggcag catgcgatac ggcgcagctg cctgcacata gactttcttc
    11581 acacgaccgc gatccataaa gtcattcaca tagctcgaac cccaggcggt ttgcagtgtg
    11641 tcgttaatat cgtcgatagc aacgcccagc gcctgagctt tacgctggtc gatatcaatc
    11701 tgcaactgcg gactgtcatc gaggccgtta tggcgcacac gggttagctc cgggttttcc
    11761 gccgccagcg ccagcaactg gttacgtgct gccatcagcg catcgtgacc tgctccagcg
    11821 tggtcctgca actccatatc aaaacccgca gaactaccaa gaccgctaat tgctggcggg
    11881 ctgctggcga taacgcgagc ttctttaatc ttgttaaacg cctttgttgc acgctcgata
    11941 atggcaaacg aggtgccggt cttactgtcg cgttcgctcc agtctttcag gcggataaac
    12001 attcgcgcca cgttttgccc gttaccccca gggccagaac caacggtggc aaacaccgac
    12061 atgatgttgt ctttttcatg ggtgaagtaa tatttctcga tttgctcaac gactttcagg
    12121 gtctgttgtt gggttgaacc gctgggcaat tgtaccgagg tagtaaacat gccacggtct
    12181 tccagcggta agaacgacgt cggcaaacgc aggaacagga acaccatgcc gccaagcagc
    12241 aggacataaa tcacaatcca gcgcaggctg cggtggagaa ttttcgccac ccctttttcg
    12301 tagcgttcgg cgttgcggtt aaacatctgg ttaaaccagg caaaaaagcc tttttgcccg
    12361 tgatgctcac cttttttcag tggcttaagt agtgtggcac acagagccgg agtgaggatc
    12421 atcgctacca gtactgacag caccatcgcc gcaacaatgg taatagagaa ctggcgatag
    12481 atggcaccgg tggtgccgcc gaagaaggcc attggtacaa ataccgccga aagcaccatc
    12541 gcaatcccga ccagtgcccc ctggatctgc cccatcgatt tacgtgtggc ttcgcgagga
    12601 gcgagtcctt cctcactcat aatacgttcg acgttttcca ccaccacgat tgcgtcatcc
    12661 accagcagac cgatcgccag taccatcgcg aacatggtta aggtgttgac gctgtaaccg
    12721 aaggcgtaaa gtacggagaa ggttcccatc aacaccaccg gcacggcgat agtgggtatc
    12781 agcgtggcgc ggaagttttg caggaacaga tacataacga ggaaaaccag ggcgatagct
    12841 tccagcagcg ttttcaccac gtcttcaatg gaggctttaa caaacgaggt tgtttcatac
    12901 gccaccttgt attccagtcc gtgcgggaag tactgcgcca gctcgtcgag acgattgagc
    12961 accagctccc tgtcgccatt tcgtttgcgc cggaggccag ttttaccccc agcccggagg
    13021 ctggcttacc attgaagcgg ctaagataat cgtatttctc cgcccccatt tcgacggtgg
    13081 cgacatcgcc cagccttacc tctgagccgt cttgattcac ccgcaaggtg atatcgcgga
    13141 actgttctgg tgtttgcagc agtgactggg cgttaatggt ggcgttgagc gcctgcttat
    13201 cgacggaagg tgtaccacca agttgcccaa ccgcaatctg cgcgttctgt gactcaatgg
    13261 catcagtgac atctttagcc gtcatctgga aactgttgag tttcgccggg gccagccaga
    13321 tacgcatgga atattgcgaa ccataggcat cgatatcccc gacgccattc acgcggctta
    13381 acgggtcctg aatattactg gcaacataat cggcaatatc ctgtttatcc atcgaaccat
    13441 cggtagagac gaaagcaatg gtcagaatat tggtatcgcc ggttttacgc accgtcacgc
    13501 cctgattttg caccgcctgc ggtaactttc gcatggctga ttgcagctgg ttttgtactt
    13561 gctgcacggc ttcgtccgga tcggtgcctg ctttaaaact taaagtgaca gatgcctgac
    13621 cggtgccact gctctgagat gacatataca tgagattatc gaggccggtc atattttgct
    13681 cgataacctg gggcacggtg ttttccagcg tctgggccga tgcgccggga tagttagcgg
    13741 tcactcgcac attcggtggc gcgagatcgg ggtattgttc aacgggcaat gaaaaaatcg
    13801 ccagggtccc tgtcagacac aacaggattg ccagcaccca ggcaaaaatg gggcgatcaa
    13861 taaagaaatt cgccattaaa agaggacctc gtgtttctgc gtatcgttgt aggcttgact
    13921 tgcttcactg tagcggcaag gtacgagcca atcgtggaga aaaaaggagt taatgtaaat
    13981 tatcatgccc ttttatactg aaccttaagt gcaagtattc tttggtcagt aggaggcatt
    14041 acatcaactg actttcctca ccctctgcag agcgaaagct aatactcact aacgtgccgc
    14101 cgccggaagg ttgtgaaaaa gtcagcgtcc cacccagccg ttccgcgcgt tcgcgcatga
    14161 tattcagacc ataatgacct tcgggttctt tcggttcacc gataccaatc ccgttatcac
    14221 ggatataaac cgtgtgattg ccgtccggcg cggtgacgca actgacggcg atttcgctgg
    14281 cgttggcgtg cttcatcgca ttcagcaccg cttcgcgaat aatttgcaac aaatgcacct
    14341 gcatttgcgc atccagcgca agggttggca gacggcagtc gagggtcagt ttggcgctgg
    14401 tttgattttg taacgtatcc agcatttccc tcaacgcgga ggggagatcc gcctgctgca
    14461 gcgtcaggcg gaaagtagtc aacagctcgc gtaactgccg ataagcatca ttcaacgcct
    14521 gggaaaaatc ggccatgata ctttgtgcgg tggcgttatc ttccggtatc gaacgcttca
    14581 gtaacgtcaa ctggatacgt aagtaagaaa gtacctgagc cagcgagtcg tgcaattcgc
    14641 gggcgatggt tgcacgttct tccatcaaca ataaatgctg aaaatgcttc tgcgcctgat
    14701 taaagtacaa accgcgtccc agcatcgacg aaacgctgtt aagcagcggt tctgatgatg
    14761 aaacgtgact attttgccag tgcagttcgc cgtaaaccgt ctcttgcatt gtcaccggta
    14821 aaatctgcat cggcaattcc gggtttggtt gcccttcgct aatccgccag ttttcaccga
    14881 catttaactc cagatattca gccgcttcat tgtcgcgaac aatctgcaaa atatggcgga
    14941 aacaatgcac atcaatctgg cttgtgttca gcgcctgcga acattgatac aacacctcca
    15001 gacgacgctt ggcctcgtgg agatcgcggg tcttttcttc tactgacgcc tccagcgaaa
    15061 ggtacaattt atgcagctcg ctcgacatct ggttaaaggt ttttgcaagc agaccaagct
    15121 cattcggcag actggtatcc agcggcggcg agtcgaactg cccgtgttca atacgttgac
    15181 tggcggtaac cagctgattc agcggggcaa ccacctgatg gcgtatacgg cgcaaagtaa
    15241 aaaagaccag cgtgaaaata ccgataccgc cagccaggga aatcgccacc accagcagca
    15301 ttttgcgttc agcgtagtgc tgtaaagcca gcacgaacag gtctatctga ttaacataat
    15361 tattaatatt ggcctgatac cacggcaaat cgcccttgct gagccgatta ttcatctcca
    15421 gccagttggc attcagatgc gcatagcgag tttttactgc ttctggcaca taccagacgt
    15481 tgagattggt taataccggt gaatgcagtg cctgctgaaa caactggcga tgtgcattga
    15541 gttgtggact gccactttgc aagtcgtagc ccaggcggta actctgcata cgcagcgatc
    15601 cggcaatatt gatagcctca gcgtcgcgca aactgctcgc cagagttagc agagcgatac
    15661 ccgtggaaag aatcgacagc agcacaatgt aaaaaaaggc ccgggccaga ctggccgaga
    15721 cgggtcgttt aacaatcaca cgcgtcttct ccacaaaaaa tcattaatgt tccagttcgc
    15781 tgaaacgcaa tttagtcagt gatgggatta tttgagctgc cacagtttat agcgaaatag
    15841 ttacttattc tgggcacatg aacattgccg attcccctgt aagtcgggta ataacaacac
    15901 tcatataaag aataaggttt ttacaaccaa aaaagaaggt cgttatgaat cgttttatta
    15961 tggccaacag tcagcaatgt ctgggttgtc atgcttgtga aatcgcctgt gtcatggctt
    16021 acaatgatga gcaacatgtc ctgagccaac accattttca tccccgaatt acggttatca
    16081 aacatcagca gcaacgtagt gcagtgacct gtcaccattg tgaagatgcg ccctgcgccc
    16141 gtagctgccc taatggcgca atcagccacg ttgatgacag cattcaggtc aatcagcaaa
    16201 agtgtattgg ctgtaaatcc tgcgtggtgg cctgtccgtt tggtacgatg caaatcgtcc
    16261 tgacacccgt cgcggcagga aaagtaaaag ccacggcgca taaatgcgac ctttgtgcag
    16321 ggcgcgaaaa cggtcctgcc tgtgtcgaga attgcccggc ggacgcgctg caactggtca
    16381 ctgacgtcgc actctccggc atggcgaaat cccgccgctt gcgcaccgca cgtcaggagc
    16441 atcaaccgtg gcatgccagt accgctgcac aagaaatgcc ggtaatgagt aaagtcgaac
    16501 aaatgcaggc aacgcccgcg cgtggcgagc cggataaact ggcgattgaa gcgcgcaaaa
    16561 ccggttttga tgaaatttat ctgccatttc gcgccgacca ggcacaacgg gaagcctcgc
    16621 gctgccttaa gtgcggcgag cacagcgttt gtgaatggac ctgcccgctg cataaccata
    16681 ttccgcagtg gattgaactg gtgaaagccg gaaacatcga cgccgccgtc gagctttctc
    16741 accagaccaa caccctgccg gaaattaccg gacgcgtttg tccgcaagac cgtttgtgtg
    16801 aaggtgcctg tactattcgc gatgagcacg gcgcggtaac tatcggcaac attgaacgct
    16861 atatttcaga tcaggcgttg gcgaaaggtt ggcgtcctga cttaagtcat gtcaccaaag
    16921 tggacaagcg ggtggcgatt atcggggcag gtccggcagg gctggcctgt gcggatgttc
    16981 tgacccgcaa tggcgtggcg gtgacggtgt acgatcgcca tccagaaatc ggtggcttgc
    17041 tcactttcgg cattccttct ttcaaactgg ataaatccct gctggcacgc cgtcgggaaa
    17101 tcttcagcgc gatggggatt cacttcgaac tcaattgtga agtgggtaaa gatgtctctt
    17161 tggattcgct tttggaacaa tacgacgcgg tcttcgttgg cgtaggcact taccgttcca
    17221 tgaaagcggg tttacccaat gaagatgcgc cgggcgttta tgacgcgctg ccgttcctca
    17281 tcgccaacac taaacaggtg atggggctcg aagagctacc ggaagagccg tttatcaata
    17341 ccgccggact taacgtcgtg gtactgggcg gcggcgacac cgcgatggac tgtgtgcgta
    17401 ctgcgctgcg ccacggcgcg agtaacgtca cctgcgctta tcgtcgtgat gaagctaaca
    17461 tgccaggctc gaagaaagag gtgaagaacg cccgcgaaga gggggccaac ttcgaattta
    17521 acgtccagcc ggtggcgctt gagctgaatg aacaaggtca cgtttgcggg attcgtttcc
    17581 tgcgcacgcg tcttggcgag ccggatgcct aggggcgtcg gcgtccagta ccggtggaag
    17641 gcagtgaatt tgtcatgcca gccgacgcgg tgattatggc gtttggcttc aatccgcacg
    17701 ggatgccgtg gctggagtcg cacggtgtaa cggtagacaa atggggccgc atcatcgcgg
    17761 atgtggaaag ccagtaccgt taccagacca ccaatccgaa aatcttcgcc ggtggcgacg
    17821 ccgtgcgtgg tgcggatctg gtggttaccg caatggcaga aggacgtcat gcggcacagg
    17881 ggattattga ctggctgggg gtaaaatcag tcaaatctca ctgatagcct gcgcagacaa
    17941 acccgacttc acagcgtaag ataattgttc atttcgcgct gtggagtcgg tatgacgcaa
    18001 caaatcaccc tcattaaaga caaaattctc tccgataact atttcaccct gcacaacatt
    18061 acctacgatc tcacgcgcaa agatggcgaa gttatccgcc ataaacgtga agtttacgat
    18121 cgcggtaatg gtgcgacgat cctcctgtac aacgcgaaga aaaagaccgt ggttctgatt
    18181 cgtcagttcc gtgtcgctac ctgggttaat ggcaatgaaa gcgggcagct gattgaaacc
    18241 tgcgccgggc tgctggataa cgacgaaccg gaagtgtgca ttcgtaaaga agcgattgaa
    18301 gagacgggct atgaagtggg cgaagtgcgc aaattatttg aactgtatat gtcgccaggc
    18361 ggtgtgactg agctaatcca cttttttatc gccgaataca gtgacaatca gcgcgctaac
    18421 gccggtggcg gtgtcgaaga tgaagatatt gaagtgctcg agctgccgtt cagccaggcg
    18481 ctggagatga tcaaaaccgg cgagatacgt gacggtaaga cggtgttatt gcttaactat
    18541 ttgcaaacgt cacatttaat ggactgaaaa ataacaataa tatttcgttg tttattattg
    18601 gctcaatccg acaaatccga ttgagccgcg ctacctgcgc aacgaagata cgacttgtgc
    18661 tgtttgtttg aacttctggg gtcgtaccgt ccatgcgcta tcgcattttc cttctctttt
    18721 ttttcgcttt gttgccgacg tctttggtgt gggcggcacc agcgcaacgg gcgttttccg
    18781 actggcaggt cacctgtaat aaccaaaact tctgcgtggc gcgtaatacg ggcgatcata
    18841 atggactggt gatgaccctg agccgcagcg ccggggcgca taccgatgcc gttttacgta
    18901 ttgagcgcgg cggattgaag tcgccggagg cgtcagaagg ggagatagcg ccacggctgc
    18961 tgttagatgg cgagccgttg gcgttaagtg gtgacaagtg gcggatttca ccatggttat
    19021 tagtaaccga tgatacggca accatcaccg cgtttttgca gatgattcag gaagggaagg
    19081 cgatcacctt acgcgacggc aatcagacca tttcgctgag tggcttaaaa gcggcgttgt
    19141 tgtttattga tgctcagcaa aagcgcgttg gcagtgaaac cgcgtggatc aagaaagggg
    19201 acgaaccgcc gctcagcgta ccgcccgcgc ctgcgctgaa agaggtcgcg gtggttaacc
    19261 caacgccgac gccactctca ctcgaagaac gcaacgattt gctggattat ggaaactggc
    19321 ggatgaatgg tctgcgctgc tcgcttgatc cattgcgtcg tgaggtgaat gtcactgcgc
    19381 tgactgatga taaagcgctg atgatgatta gctgtgaggc aggggcctat aacaccattg
    19441 atttggcatg gattgtgtcg cgtaaaaatc cgctggcctc gcgcccggtt cggttgcgtt
    19501 tgccgttcaa caacgggcag gagacgaatg aactggaact gatgaacgca acatttgatg
    19561 agaaatcgcg tgaactggtg accttagcga aagggcgcgg attaagcgat tgtggcattc
    19621 aggcgcgctg gcgctttgac ggtcagcgtt ttcgcctggt gcgttacgcc gcagaaccca
    19681 cctgcgataa ctggcatggg ccagacgcct ggcccacgtt gtggatcacc cgttaaacaa
    19741 aatggcgggg acatgtaggc cggataagac gctgtaggcc ggataagacg cgtcagagtc
    19801 gcatccggcc tacagcgtcc ggcaatcagc atccggcgat caccatcagg cacctttcac
    19861 tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa agaacgggaa
    19921 cagcttatcc gccggagcag attccccata acctgtcatc ccaacaatcg cccctttcaa
    19981 accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc gagccgcaac
    20041 gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa tatcagtcga
    20101 gggcagggaa actacgcgta cattgcgacc ttctcccgct aatttctctg ccgcttgcag
    20161 ggggatttcc atctctgaac cggtggcaat caaaataata tctggcttac cgccgctgtc
    20221 tttcagcaca taaccgccac gggcaatctc ttttacctga tccggcgtac gttccacttg
    20281 ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct caaccgccag
    20341 cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga aatttggcgt
    20401 taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt cgcccaggcc
    20461 aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg ccatccgcgc
    20521 ggcgttacgg gcgtattcaa caaacatcag gaacgtggcg gtatacggca caaagccgcc
    20581 gtggtgcgcg atgccgttgg ctatagcggt catgccaaat tcacgcaccc cgtagtgaat
    20641 gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg tcaggttgct
    20701 ggagccagat ccgccgaacc gccgagcaac tcaggcagca tcggc
//
LOCUS       DAJVOY010000061        20645 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVOY010000061 DAJVOY010000000
VERSION     DAJVOY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.061"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..866
                     /locus_tag="OQL90_001968"
     CDS             <1..866
                     /locus_tag="OQL90_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCS1368756.1"
                     /translation="WQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNV
                     YATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQ
                     DEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIING
                     AGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFT
                     LEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQPKLRG
                     NVQTAQREVAP"
     gene            863..1952
                     /locus_tag="OQL90_001969"
                     /pseudo
     CDS             863..1952
                     /locus_tag="OQL90_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(2040..3338)
                     /gene="exuT"
                     /locus_tag="OQL90_001970"
     CDS             complement(2040..3338)
                     /gene="exuT"
                     /locus_tag="OQL90_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCS1368757.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3821..5233
                     /gene="uxaC"
                     /locus_tag="OQL90_001971"
     CDS             3821..5233
                     /gene="uxaC"
                     /locus_tag="OQL90_001971"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1368758.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5248..6735
                     /gene="uxaA"
                     /locus_tag="OQL90_001972"
                     /pseudo
     CDS             5248..6735
                     /gene="uxaA"
                     /locus_tag="OQL90_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6818..7369
                     /locus_tag="OQL90_001973"
     CDS             6818..7369
                     /locus_tag="OQL90_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1368759.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7374..8618)
                     /gene="sstT"
                     /locus_tag="OQL90_001974"
     CDS             complement(7374..8618)
                     /gene="sstT"
                     /locus_tag="OQL90_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1368760.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9234..10199)
                     /gene="alx"
                     /locus_tag="OQL90_001975"
     CDS             complement(9234..10199)
                     /gene="alx"
                     /locus_tag="OQL90_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1368761.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10481..11467)
                     /locus_tag="OQL90_001976"
     CDS             complement(10481..11467)
                     /locus_tag="OQL90_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1368762.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11546..12229)
                     /locus_tag="OQL90_001977"
     CDS             complement(11546..12229)
                     /locus_tag="OQL90_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1368763.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12306..12809)
                     /locus_tag="OQL90_001978"
     CDS             complement(12306..12809)
                     /locus_tag="OQL90_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1368764.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12894..14030
                     /gene="rlmG"
                     /locus_tag="OQL90_001979"
     CDS             12894..14030
                     /gene="rlmG"
                     /locus_tag="OQL90_001979"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1368765.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14315..14629
                     /gene="higB"
                     /locus_tag="OQL90_001980"
     CDS             14315..14629
                     /gene="higB"
                     /locus_tag="OQL90_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1368766.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14626..15042
                     /gene="higA"
                     /locus_tag="OQL90_001981"
     CDS             14626..15042
                     /gene="higA"
                     /locus_tag="OQL90_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1368767.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15087..17105)
                     /gene="fadH"
                     /locus_tag="OQL90_001982"
     CDS             complement(15087..17105)
                     /gene="fadH"
                     /locus_tag="OQL90_001982"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1368768.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17531..19882)
                     /gene="ygjK"
                     /locus_tag="OQL90_001983"
                     /pseudo
     CDS             complement(17531..19882)
                     /gene="ygjK"
                     /locus_tag="OQL90_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19899..20645)
                     /gene="ygjJ"
                     /locus_tag="OQL90_001984"
                     /pseudo
     CDS             complement(19899..20645)
                     /gene="ygjJ"
                     /locus_tag="OQL90_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
BASE COUNT         5030 a         5413 c         5427 g         4775 t
ORIGIN      
        1 catggcagtt atcgcatacc ttttataatg attcattcag ccataaagaa cttggcgtaa
       61 ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt tgggatgggc
      121 aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat catgatcatc
      181 tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat ggcgttaatt
      241 tgggagccag tggtacagac gatttgctgg gtgcggtatt ggtggatgtg aaaggcttct
      301 ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca ggcagccgaa
      361 ctttgcagct tggtcaaagc gacagtgtgt tgttccctta tcctggattt cagtctggtt
      421 ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac atcattaacg
      481 gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg
      541 ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc
      601 cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga tttacacttg
      661 aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc cttaagtgtc
      721 cactgagtgt tgaaaagaaa cgcgccagca ttcgttacag cggagatgtt acttgtagtg
      781 tggtgactta ttcacaatta ccggagttga aacagcctaa attacgtgga aacgttcaga
      841 cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gcggcgatat tgggcttgtt
      901 tggcacgctc actggcgttc aggcagctcc tgacgtgacc agtgaaatta cgtatgattt
      961 ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag gaaataatgg
     1021 atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag ctaatggaaa
     1081 tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt ggccggtaaa
     1141 actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgctag gatgcaaggg
     1201 ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt acagaagatg
     1261 gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag cagacagaga
     1321 tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat tcgaacagcc
     1381 cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac catattgacg
     1441 tgttcttccc ggagttcgcc cacgccacgc cgagggtgca gttagacttg catccaacag
     1501 gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg tgcctgtacg
     1561 atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa gggcgaccag
     1621 ctgcagggcg cagagacggt gacttctcta tctatcgtca gggaggaacc tctaccgacg
     1681 agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt gggcaaattg
     1741 atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaaagt gtgcgtccgg
     1801 tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg gctctggcag
     1861 tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa ttgtcagtaa
     1921 tctttacgcc ttccttgcca accatcaatt aaccctgcaa agcggacaaa cgcttttttc
     1981 tatataaacg acgaagccgc ctgataaggc ggcttcgtcg ttataactta cgttaaacgt
     2041 taatgttgcg gtgcgggatc gttatgtgat tcctgcgcta cctcaatcgc cggtttgttc
     2101 tgcaacacgg tccagataac cagcgcacct aacaggtcga acactgccag tactgcgaac
     2161 agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgaaaa cagcgtactt
     2221 gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac
     2281 acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgagcaaa accaccgata
     2341 cacagcagca taattgcgac atacgggttg gtgaacaggc cgatcatacc cgggccaatc
     2401 atcagcactg cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac
     2461 caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc agcaaacagc
     2521 atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac tttaaacatg
     2581 aacagcggga tccacgcgtt aaaagtaccc caggctggtt ctgccaggaa acgcggcagc
     2641 gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt cttcgccgtg
     2701 ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc atcggtcaga
     2761 tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca gataaagctc
     2821 aatgcaccgg agatgataaa tgccatctgc cagctgtgca ttacgattgc ccataccacc
     2881 agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc taccgcgatg
     2941 gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg gatcatcgct
     3001 gcttccgcgg caccgactgc accacgagca atcgccaggc cacccctgct accagccagc
     3061 gcggttgcac cacagaatac ggcccacagt acagcaaaca ttgcataacc gattttcgta
     3121 cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc agcagaatag
     3181 gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat cagagttggc
     3241 gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccaag cgtcaccagt
     3301 gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat cgttttgtta
     3361 tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt tacgaccggg
     3421 taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg ctaattcggc
     3481 ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt tgcattccgt
     3541 cgacttatga tttgcgacgg cagaaagatg acttgtcata caactttaaa aggtgagagc
     3601 catcataaat gtgcgaatat ttgtagggac attacctgac gacagcgagg ccagtactgg
     3661 cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg tttattgatc
     3721 taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt cagcatcgca
     3781 ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt ttatgactga
     3841 agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg caaaagacca
     3901 gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag actatcgttt
     3961 taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg ctatgcgtac
     4021 caacggtgtg gccgagcgtc tgtgtaccgg tgatgcgtct gaccgtgaaa aatttgacgc
     4081 ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga cgcacctcga
     4141 actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg ccgatgaaat
     4201 ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg gcatcatgca
     4261 gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc tggagcatca
     4321 cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct ggcgtccgga
     4381 caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc tgggcgaagt
     4441 ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca aacgtctgga
     4501 tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg tgatgtttgc
     4561 tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg gcgaaaccct
     4621 gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg gtgccgaata
     4681 tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata acaacctgcg
     4741 tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc gtccgatggc
     4801 agaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc cgaaaaccat
     4861 tctgtactgc ctgaacccgc gcgataacga agtgttgggc accatgatcg gtaacttcca
     4921 gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta acgatcagaa
     4981 agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtttgctga gccgctttgt
     5041 cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat acttccgccg
     5101 cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg cggacatcaa
     5161 cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact acttcgccat
     5221 tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc gctggataac
     5281 gtcgcggtcg ctttagcgga tttggctgaa ggcacagaag tcagtgtcga taaccagact
     5341 gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga tatcgcaaaa
     5401 ggggccaatg tcatcaaata tggcctgccg attggttatg cattggcgga tattgcggcg
     5461 ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga tcagtatcgc
     5521 tatcaacctg attttcagga tctgcctgcg caagcggcag atcctgaagt gcagatctat
     5581 cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc aaccgtgggc
     5641 tgtgtcaacg gcatcgcgcg gcagatccag aaccgttttc tgaaagagac caacaacgct
     5701 gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca gctgggcgac
     5761 gatcacatta atacccgcac catgctgcaa aacatggtgc gccacccgaa cgcaggcgca
     5821 gtgctggtga ttggtctggg ctgtgaaaac aactaggttg ccgcattccg tgaaacgctg
     5881 ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga tgatgagatc
     5941 gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga taaacgcgag
     6001 ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga cggtctttct
     6061 ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc taacggcggt
     6121 actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct gatggaccat
     6181 tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt taaacagtac
     6241 tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa agcgggcggt
     6301 atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc cagtgtcgtg
     6361 gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag ggctgaactt gttaagtgcg
     6421 ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca tatggtgctg
     6481 ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa aatcgccacc
     6541 aacagtgaac tggcggcgaa gaaaaaacac tggctcgact ttgacgcggg tcagctgatc
     6601 cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt tgagtttgcc
     6661 aacggtaaac aaacctgcaa cgagcgtaac gacttccgcg aactggcgat cttcaaaagc
     6721 ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc ataatgtgaa
     6781 gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt ggctggccca
     6841 gggcgtgggt ttcatcgcct ttctgattgg tatcacaaca tttttcaatc gtgacgaccg
     6901 tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt tttttcttct
     6961 gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat tgattacctt
     7021 acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg gaattggcct
     7081 cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg tcagtaccag
     7141 ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt caacgtgttg
     7201 ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg agggtagttt
     7261 tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa ggggaattga
     7321 tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa caattaatta
     7381 cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc agtgaacagc
     7441 acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc gatgataaag
     7501 ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt acaggccagc
     7561 gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca cagagaagcc
     7621 accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt attaaccgca
     7681 gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt ggcacccagc
     7741 ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag tgccatattc
     7801 accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct ttcgcgcagg
     7861 cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag caatgggtta
     7921 accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg cgcgtagccc
     7981 cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa aataccaatc
     8041 ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacag atcgttgacc
     8101 aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat tgcccacacc
     8161 aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac catgctcatt
     8221 accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc cgcgctactg
     8281 gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag agcagcagag
     8341 aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg cccgtgctgg
     8401 tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac ggctttcagt
     8461 gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt tgagatccat
     8521 gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct gccatgagcc
     8581 agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt
     8641 gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa agtgtttgca
     8701 aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat tttaaagaga
     8761 ggtcatattt tgcaacttaa ttgttacatt tgtttgacat aaacagaggg aaggactggc
     8821 tactaaggct gattagataa catatgggca aaactatttg gaatatgaga acggttgcgc
     8881 tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag ggggcagcca
     8941 atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct gcgcgattcc
     9001 ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg taagatgcgc
     9061 cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg cgattttgac
     9121 gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccaattaca taattaatag
     9181 ctcacctttg tctgggatga gttatctcta ggaatggctt aatatacagc gaatcattct
     9241 ccccgctgct tatcatgccg ataattcacc ctggcgttga taataaacgt catcaccaga
     9301 atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc gacaatcagc
     9361 atcttgatac cgataaacac caggatcacc gccaggccat atttgagcat cgagaaacgc
     9421 tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggatcgc aaacaggttt
     9481 gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat gctatccacg
     9541 gcgaaaatca cgtcgctcaa ttccaccaga atcagtacca gcatcagcgg tgtggcgtac
     9601 agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt catgcgcaaa
     9661 tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc ttcatgggcg
     9721 agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag gatccagtcg
     9781 aactgcgaaa ttagccagct gccagtgaag atcatgatgg tacgcagaac aatcgccccg
     9841 agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa atagctgaac
     9901 aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag ataacccgtg
     9961 agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cggtttgcac cagataccac
    10021 cagaaagcgg cgttaaacag taacgacagc gtcacccaaa ccagcgacca ggccgcagcc
    10081 tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc gatagccagc
    10141 ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac agtattcata
    10201 gaagttcctt acacataaaa aaaacggcca acgtcggaag acgtcagccg ctgcttttta
    10261 tgcatagacc tcgccttccg gcaaggtctc acttacaaca aaaaagaata cgtctgcgta
    10321 ttcctttcgg gttgcccggt gaccggatgt ggtttttcac acatcgtaat gacgatcgac
    10381 cggcaatgaa gttactcccc tttgcaggta acaaagtatg acagccattc agccagtcaa
    10441 tggtctgtta catacctttt acactgtttt actttggcaa ttatagtttt acgctatctg
    10501 ccggaaaaat caccccagtc tggcggcgga tctcagtcag cagtttggcg gtgatgcgac
    10561 tgaccgccag ccctggatga tccaccagat gctcatccac cagggtagca aacagctctg
    10621 cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc tggctgccac
    10681 gcggcacgaa gcacactttc tggcattcag acagtttttc aatcaccagc gatcccgctt
    10741 cgccctgaat ctcgctttcc aggacagaat cactgacctt tgagtgcagc aacgtaacgc
    10801 tgaaatcacc gtaatccatc accaccacgc cgtgggcatc tacgccgctt gccaacaaac
    10861 tggcggttgc ctgcacgctt ttcggttcac cgaataatgc caccgccgac gccaggcagt
    10921 aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg ttgggattct
    10981 caccatccag gtaacgttga taccgcgagg aatactggca atagttgaaa aagactttac
    11041 gcaatttgcc gactttcggc agcgcctggc gcagcaaatg aaagttcggc aggctggcgg
    11101 ttttaaatgc ctcaaacagc accacctgat tttcccgcgc acaggcaatg gcggcatcca
    11161 cttccgccag attcgacgcc agtggtttct cgcaaatcac atgaatttta tggctaagga
    11221 aaagttgtgt ctgggaaaag tgcagggcat tcgggctggc aatatacacc gcgtcaatgg
    11281 catcgctttc cgccatcgct tccagcgagg taaacagatg ctcgacagaa aaatcattgg
    11341 cgaagtgctg ggcctgttca aggctgcggg aatatacggc ggttaacttg tatttaccgc
    11401 tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc acagcgaaac
    11461 gtatcataac gcgtggatac tccataaagg gattctgtcg tcaatgtagc acgccttttt
    11521 catcttcgac gtgcgcagat acgcattatt cttttgccgg acagccatgc tcactaaacg
    11581 gcccaatcat gaccgatggc ccgagatatt tcggctggct atggaggatt ttggcgtcaa
    11641 tcacggcgct gacacgagcg agtttctctc gcagttgagt ataaaaaccg tgcttcatat
    11701 tgaggtcagg cgcgttaaaa ccgatagcgt cgatgctgta ctgttttgcc agccagatgg
    11761 cgcgttggtt atgaaattct tgcgagataa tggtgatatg gttttcacca aaaacctttt
    11821 tggcacgcac taccgaatcc agcgttgaga atccggcata gtcgcagaag atcactttcg
    11881 ccggtacgcc tttagcgatc aaagccttct gcatacctga tgcttcatcg taatttttac
    11941 gtccgttatc gccacttacc agtagccatt tcacttttcc ggcgtggtac aacgctgctg
    12001 cggtatcaat acgtcgggta aagtagcgat tacccggcct ggcccccagc aataagccca
    12061 cgttacgcgc cggaactgcg ttgacatcgc tccaggtcag ctgcttgctg gcgttgacca
    12121 ttactcgatc ggcgataaaa atggtcgctc ctgccacaag taacagtaag caggcaatct
    12181 ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg gggctgcatg gtcgttatcc
    12241 ttaacgaaag gggggaggtc gattttaggc gctaaagaaa gaaaagataa cgcctgacat
    12301 cattttcaga ttttgtcctg cccaagcgac aactgcgtta gccacagccg ggtgtcgaac
    12361 tccagctggt ggtactgcgg ctccatatgg cagcacaact ggtaaaacgc tttgttatgc
    12421 tctttctcct tcaggtgcgc cagttcgtgc accacgatca tacgtaaaaa cggttccggc
    12481 gcgttgcgaa acacagtagc gacgcggatc tctgcttttg ctttcagctt gccgccctgc
    12541 acacgagaaa cagcggtatg taacccgagc gcgtttttta gcacatggat cttattgtca
    12601 tacatcactt tattgatcgg cggggcattg cgcagaaact gattttttag atcctgagta
    12661 tattgccaga gggctttatc ggtggcgtag tcgtgtgttc ccggatagcg ttttgccagc
    12721 acatcgccca gacgctgttc gttaatcagc gttcgcacct gggaaagtag ctgctcggga
    12781 taaccctgaa gataagtaag attgctcata accgcccacg aaaaagagaa aaagggtata
    12841 ctcacgcacc cttttcaggg gcaacgtcga aattttatca ttcaggaggg ccgatgagcc
    12901 acttagacaa cggtttccgt tcactgacac tacaacgttt tccggcgacg gatgacgtta
    12961 acccgctaca ggcgtgggaa gcggcggatg aatatttgct gcaacagttg gacgacacag
    13021 aaatccgcgg cccggtgttg atcctgaatg atgcctttgg tgcgttaagc tgcgcgctgg
    13081 cggaacataa gccgtacagc attggcgact catacatcag tgaactggcg acgcgcgaga
    13141 atttacgcct caacgggatt gatgagtcga gcgtgaagtt tctcgacagc accgccgact
    13201 acccgcaaca gccgggtgtg gtgctgatca aagtgccgaa aacactggca ttgctggaac
    13261 aacaactgcg tgcgctgcgc aaagtggtca cgtcggatac acgtattatt gccggtgcca
    13321 aagcccgtga cattcacact tccacgctgg aactgttcga aaaagtgctc ggcccgacca
    13381 ccaccacgct ggcatggaaa aaagcgcgcc tgattaattg cacgttcaat gaaccgccgc
    13441 tggccgatgc gccgcagacc gttagctgga agctggaagg tactgactgg actatccata
    13501 accatgcgaa tgtcttctcc cgcaccgggc ttgatatcgg cgcgcgcttc tttatgcaac
    13561 atctgccaga gaatctcgaa ggggagattg tcgatctcgg ttgcggtaat ggcgttattg
    13621 gtctgacgct gcttgataaa aatccgcagg cgaaagtggt gtttgtcgat gaatcgccga
    13681 tggcggttgc ttccagccgt ttgaacgttg aaaccaacat gccagaggcg ttggatcgca
    13741 gcgagtttat gatcaacaac gcgctctccg gcgtggagcc tttccgcttt aatgctgtgc
    13801 tctgcaaccc gccgtttcac caacaacatg cgctgaccga taacgtcgcc tgggagatgt
    13861 tccaccatgc ccgccgctgc ctgaaaatca acggcgagct gtatattgtt gccaaccgtc
    13921 acctggatta cttccataaa ctgaagaaga ttttcggcaa ctgcaccacc atcgccacga
    13981 ataataaatt tgtggtgctg aaagcagtga agctggggcg tcgtcggtaa gattgggagt
    14041 tacctgccgg atgcggcgtt aacgccttat ccggcctacg ttcagcacgt tactcccagg
    14101 ccggataaga cgcggcaagc gtcgcatccg gcaatgtctg cccagttctg gaatcgcgct
    14161 tccataaaac aaaaaatccc tctcaccatc ctctacattc tcttgtttag cgtttttcta
    14221 cgtttattct tccgtcacac agataaattc atacgttgcc aatctgtcaa cggatgttat
    14281 catattgcca atttgtcaac ggacgtgatg aagaatgcac ctgataactc aaaaagcatt
    14341 gaaagatgct gcggaaaaat acccgcaaca taaaacggag ttggtggctc tggggaacac
    14401 gattgctaag ggatatttca aaaaacctga gtcattaaaa gcagtattcc catctctgga
    14461 taacttcaaa tatctggata agcattatgt tttcaatgtt gggggcaatg aattacgtgt
    14521 tgtagcaatg gtcttttttg aatcgcaaaa gtgctacata cgtgaagtta tgacgcataa
    14581 agaatacgat ttctttaccg ctgttcatcg tactaagggg aaaaaatgat tgcgattgcc
    14641 gacatcttgc aagcaggaga aaagctaact gcggtggtac cttttctggc gggtattcag
    14701 aacgaggaac aatacaccca ggcgctggaa ctggtagatc atctgctgct caacgatcct
    14761 gaaaacccct tgctggatct ggtgtgtgcc aaaataaccg cgtgggaaga atcagcgccc
    14821 gaatttgcgg aatttaatgc catggctcaa gccatgcctg gcggtatagc cgtgattcgt
    14881 acccttatgg atcaatatgg tttaaccctt gccgatctgc cggaaattgg cagtaaatct
    14941 atggtgtcac gcgttttgag cgggaagagg aaattaacgc tggaacacgc taaaaaattg
    15001 gcaacgcgat tcggcatttc tcccgccttg tttattgatt aacgcgacgt gccggatgcg
    15061 ttgcatccgg cgcttcaaat aacgatttaa atctccagcg ccagccgtgt tccctgggca
    15121 attgcccgtc gtgcgttcag ctccatagcc acatcgcagc cgccaattaa atgcacagtt
    15181 ttcccgctat caatcagcgg ttgcgccagc gcgcggtttg gctcttgccc tgcgcagatc
    15241 accacattgt ccacagctaa tacctgcgtt tcgccgttga tcaccacatg cagcccgtca
    15301 tcgtcaatct tctgataact tacgcctggg atcattttca caccccgcga gagcagggtg
    15361 gtgcgatgga tccagccggt ggttttgcct aacccctgtc ccggtttgct ggctttgcgc
    15421 tggagcatca caatttgccg tgggctacgc gggatctgca ttccctgcgg gcttaagcca
    15481 ccagcctgtt gtaggctgct gtcgatcccc cactcattac agaacctggc gatattctgg
    15541 ctggttgatt cgcccggctg acttaaatac atcgccgtat caaaaccaat cccgccacaa
    15601 ccgatgatgg caaccttgtt gcctaccggc gctttgtcgc gcagtacatc gagataactc
    15661 aataccttcg gatgatcgat cccgtcgata ggcggaacgc gcggcacgat cccactggcg
    15721 aggatcgttt catcgaacgc ctgtaactga tccgccgtca cggtgtgatt gagtttcagc
    15781 gtcacgcccg tcacttcgat catccggtgg taatagcgca gcgtttcgta aaactcctct
    15841 ttgccgggga tctgtttggc gatattaaac tgcccgccgc tctcgctgtg ggcatcaaac
    15901 aatgttacct gatgcccacg cgccgccgcg ttaatggcaa acgccagccc agcaggtccc
    15961 gcaccgacca ccgccagatt ttttttctgc acggcaggaa ggattggcat tttggtttcg
    16021 tggcaggcgc gaggattcac caggcacgag gtgactttgc caacgaagat ctgatcgagg
    16081 caggcctgat tgcagccaat gcaggtgttg atctcatcgg ctcgccccga ttgcgctttt
    16141 gacagcagtt ccgcatcagc aagaaacggt cgtgccatcg ataccatatc ggcatcgccg
    16201 cgcgagagaa tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt taccagcggc
    16261 agcgagacgt ggcctttcag tttgcgcgtg acccagctaa atgcgccgcg cggcacgggc
    16321 gtggcaatgg tcggaatacg tgcttcatgc cagccaatgc cggtgttgat aatggtcgcg
    16381 cccgccgctt caatggcctg cgccagctct accgtttcgg caaaagtccc gccgtcttcg
    16441 accaggtcga gcatcgacag tcggtagata ataatgaagt cgttgccgac gcgttcgcgc
    16501 accgcacgca ctacttctac ggcaaatcgc atccggttgc ggtaatcgcc gccccactgg
    16561 tcactacgct gattggtgtg cagcgtcaga aattcgttga tcaaataccc ttcggacccc
    16621 attacctcta caccgtcgta tcctgcctcc cgtgccagtt gcgcgcagtg ggcgaaatcg
    16681 tcgatcagtt gcaggatctc ttcatgggtg agttcgtgag gaacgaaacg gttgatgggg
    16741 gcctgcaatg cggagggagc gaccagatgc ggttggtagc tgtagcgccc ggtatgcaaa
    16801 atttgcaggg ctattttgcc gccttcctga tgtaccgctt cggtaatggt gcgatggtgt
    16861 gggatctggc tggcgtcgtt gagcattgcg ccaccttcca tgccaacgcc tgttaaatct
    16921 ggtgcaatgc cgccgctgac aatcagcgcc acgccgtgac gggcgcgttc ggcataaaac
    16981 gctgccagcc gctcggcacc gtccgggtat tcctccagcc cggtgtgcat tgagcccatc
    17041 aacacgcggt tttttaacgt ggtaaaacct aaatccagcg gggcgaacag cgacgggtag
    17101 ctcataaaat tgtccagtat gtaaaataat tgttatgtgg tcggatgagt tctaatttag
    17161 ccgtcgccgg agaaaaggga aaaggggagt gcggggtttg tgatgggatt caaaaagaat
    17221 acgcccggtg gtaacgccgg atgcgacgct tgccgtgttt tatccggcct gggggattgc
    17281 acatgtaggg cggataaggc gttcacgccg catccgccag tggcgcggtg cagatgccgg
    17341 atgcgacgct tgtcgcgtct tatccgacct acgggaacgc acatgtaggg cggataaggc
    17401 gttcacgccg catccgccag tggcgcggtg cagttgccgg atgcaacgct tgccgcgttt
    17461 tatccggcca ggaggattgc acatgtaggg cggataaggc gtttacgccg catccgccag
    17521 cagttgcaaa ttattgctta cggaaaaaat cgttatacaa catatacaaa tgcgcggcac
    17581 tccaggagaa atttggtgcg ccttgctgcg cgccagtcag cgggttgtaa ttctcctgaa
    17641 ttgggccatc ggcggttaat cctttggcgt gccggaagaa cgtatccgcc agcttcaggg
    17701 catcatcacg ataaccgtaa cgctccatcc ctttcagagc aaaccagaac tgatccaccc
    17761 atacgcgccc gcgccagtag atatcagcgc caaaagccgg attggttaac gccgccgttc
    17821 ccagcgggac aaaggtgttg aactctttag gatcgagcat caccttcacc accgcgtcgg
    17881 cattggcctg cgttgccgca ccgttaaaca gcggcgacca gccttccggc cctttaccgc
    17941 gctcaacaat cggtttgccc gcgcagccgt tcgccagtgg tttatcttca atacgtacgt
    18001 catagtagaa ctgggtagtc gggtcgaaca tacaggtgtt gatgtagtcc gcgagctgct
    18061 gtgccaactg gcgatagcgt ttggcctctt ctggcttacc gaggatggtt gccatctccg
    18121 ccagataatg gttatcgctg tacatatagc tggcctgatc caccgactcc tgcaatagcg
    18181 agtagcccag caacgttccg tcctgactgc ggttttcggc gaatttcacc gtccagtcgc
    18241 tacgtttgcc accattagcg acatatttat ccaactgttc tttgtcgata aaaccaaaga
    18301 cggcggcatc atcacgcccc gattcccacg acgcagcgac ctgtgccgga atttccagac
    18361 tgtcgtactg gcctttctcc accacgcggg cgtagttgtt cagcccagac tgcgtctctt
    18421 ctttgttgcc tttcttcacc gcaaacagca tctcgccgct ctcagtgttg tgggctttgt
    18481 cgcgggtcgc gccatattcc ggcacgccgt tgccgttgtg atcgcggtta cgtaaccacc
    18541 agtcgtgata ggcgaccagt ttcgggtaca tctctgccaa ccaggcttta tcctgggtga
    18601 cgttgtacac ttctatcacc gaccaggcgg caaggctggg tttggtatta cgttcgttcc
    18661 agttgccgcc atcgccacca cgctcggggc taaggttcca cgctatcagg tcggggacaa
    18721 agcccacatc ctgcggacgc acgcggtcgc caggctggat ctgccaggag aagaccgcgc
    18781 ggatattctc tttggcgatg tccggattga aatgcgccat cgcaaacgcc tgcttctagg
    18841 tatcccacgg ccaggtctga ttgccggaga accagcgccc ggtcaccgac ggtgtaacgg
    18901 tgttaaattt caccgcaccg cccggtgagc gccagttacc gttgagcgtt tcgatcgctt
    18961 tcaccgcgac gcgcgtctgt tccggcgtcg catccggatt ggttaaccct ttcttcaggt
    19021 attcttccca gcgttgctgc gaggcggtga gataaaacgc cggacgcgcc agaatatcgc
    19081 ggatctgcat ttgctctttg ctaacttcct gagcggtcag cagatgggaa taggtggtgt
    19141 agagcgtggt cgaaccgttg atatgcgcct tactggtaaa gcgattaccg ttgatttcag
    19201 tctgcaccgg cagggattta tgcacctgat attccgattc gccggaggtc agcagatccc
    19261 aggtggcgcg cactttgcca aaggtgactt tcaggccatc acgggtggcg ctgattttgc
    19321 gctgatagtc agggtattcg ccagcaatgg ttttatcgga aagcggtttc ccttctttcg
    19381 cttccagttt ttccagcagt tcgccgtccc acaccagatc cagcggttta ttgctggtga
    19441 ttttggtttc cagtagtgac gtgcgcggcg tggcgaagcg cagagtcatt tcgacttgca
    19501 catcttttgc tgtcagtttt tgcaccagcg caccgggaat actgtatgcc tccagcgtaa
    19561 agtcgacttt cttgccgtcc tgccagacgg tcaggcggtc gaaattgctg gccataaagt
    19621 tgatgtactc ttccgtcagc agcgcaacgc ccggaaagcc gcccatggtg ttagggccgt
    19681 ctggcaacag atgaccatgc caggctccga gatcgaaaaa cggattaaaa cgctggtgat
    19741 cgtcgtaatc gtaatccttc atgtactgcg gcgcgccagt acggttaatc acgtttttat
    19801 aattgtcggc gttagcggca tgggcggaaa aacttatcag cagagcgcag gttactggcg
    19861 ttaaaatagt ttttattttc atgaaattac agtccttatt accagtagaa atattgcatc
    19921 aggaagattt ggtcgctggg atcgtgagag gcatttttca tgtagaagcg gctatcaaaa
    19981 gccgtggcga aacgtccgcc gccgtattcg taccagatac caaactgcat gaaagaggtg
    20041 gtttcatcgt cagcgtggtc agggcgatgt ttggcgtagg tgtaagcggt ggagagataa
    20101 acgcccttta acgcttcata catggcgctc accatcatcc cactttcact tcccggtacg
    20161 tccagcccgt cggcgcgtcc ggtatggtgc caggcgcgag cggcaaagtt aggagttagc
    20221 aggcccgtga gatagagctg tcctgtgccg tcggtgattt ccagaccgtt catccaggtg
    20281 atgccttcgg caaggtcata ctggatgtaa ccgttcatca ttgccggata ggtgtatttg
    20341 tcgtcatagc gatcgtattt gccaaaatgt agccaggctt tgctttcatc atgatgtccg
    20401 gcaggagtcg cggtgacgct ataacgaaag ttgccgctta agttctgtac cttcagcgca
    20461 gccataatgt cgcgggtatt gggaatgacg tagcccagat cgggggtgaa atcgccccac
    20521 cattgcaggt catccagcga ggaatcctaa cgcagactga gcatgatttc ggtgccatca
    20581 ttggtacgat aaccgccata gaaacggtta attccgccct cgaaaccacc ccagctatgg
    20641 tctgg
//
LOCUS       DAJVOY010000062        20594 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVOY010000062 DAJVOY010000000
VERSION     DAJVOY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.062"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(57..482)
                     /gene="nudI"
                     /locus_tag="OQL90_001985"
     CDS             complement(57..482)
                     /gene="nudI"
                     /locus_tag="OQL90_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1368769.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYTWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            761..1303
                     /locus_tag="OQL90_001986"
     CDS             761..1303
                     /locus_tag="OQL90_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368770.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARSTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1403..2605
                     /locus_tag="OQL90_001987"
                     /pseudo
     CDS             1403..2605
                     /locus_tag="OQL90_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2825..3607
                     /locus_tag="OQL90_001988"
     CDS             2825..3607
                     /locus_tag="OQL90_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCS1368771.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3622..4827
                     /gene="rhmD"
                     /locus_tag="OQL90_001989"
     CDS             3622..4827
                     /gene="rhmD"
                     /locus_tag="OQL90_001989"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCS1368772.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4884..6173
                     /locus_tag="OQL90_001990"
     CDS             4884..6173
                     /locus_tag="OQL90_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1368773.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6191..6994
                     /gene="yfaU"
                     /locus_tag="OQL90_001991"
     CDS             6191..6994
                     /gene="yfaU"
                     /locus_tag="OQL90_001991"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCS1368774.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7035..7937)
                     /locus_tag="OQL90_001992"
     CDS             complement(7035..7937)
                     /locus_tag="OQL90_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1368775.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8130..9320)
                     /gene="glpC"
                     /locus_tag="OQL90_001993"
     CDS             complement(8130..9320)
                     /gene="glpC"
                     /locus_tag="OQL90_001993"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1368776.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9317..10576)
                     /gene="glpB"
                     /locus_tag="OQL90_001994"
     CDS             complement(9317..10576)
                     /gene="glpB"
                     /locus_tag="OQL90_001994"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1368777.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10566..12194)
                     /gene="glpA"
                     /locus_tag="OQL90_001995"
     CDS             complement(10566..12194)
                     /gene="glpA"
                     /locus_tag="OQL90_001995"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1368778.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12467..13825
                     /gene="glpT"
                     /locus_tag="OQL90_001996"
     CDS             12467..13825
                     /gene="glpT"
                     /locus_tag="OQL90_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1368779.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13830..14906
                     /gene="glpQ"
                     /locus_tag="OQL90_001997"
     CDS             13830..14906
                     /gene="glpQ"
                     /locus_tag="OQL90_001997"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1368780.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14948..15190)
                     /locus_tag="OQL90_001998"
                     /pseudo
     CDS             complement(14948..15190)
                     /locus_tag="OQL90_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15369..16019
                     /gene="inaA"
                     /locus_tag="OQL90_001999"
                     /pseudo
     CDS             15369..16019
                     /gene="inaA"
                     /locus_tag="OQL90_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16073..16327)
                     /gene="yfaE"
                     /locus_tag="OQL90_002000"
     CDS             complement(16073..16327)
                     /gene="yfaE"
                     /locus_tag="OQL90_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1368781.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16327..17457)
                     /gene="nrdB"
                     /locus_tag="OQL90_002001"
     CDS             complement(16327..17457)
                     /gene="nrdB"
                     /locus_tag="OQL90_002001"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1368782.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17604..19889)
                     /gene="nrdA"
                     /locus_tag="OQL90_002002"
     CDS             complement(17604..19889)
                     /gene="nrdA"
                     /locus_tag="OQL90_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1368783.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4843 a         4989 c         5525 g         5237 t
ORIGIN      
        1 gaattcgtta accactaaat tttcaatctg acagccagtg atggctgtca tcattgttaa
       61 agaagacctt tcaaacgtaa cgtttttcgg gtggcaacat tcaaatcata atgcaccaga
      121 tcttcaggtt ttacccacgt gtagtcctga aactcttcgt ttattttcac ttctcggttg
      181 gcagaaacgc agtcaaaaat caggtaaatc atataaatct cttccttgcg accatctgca
      241 tacgtcttgg tgcgaatatc atcgctgaag gtccacggcg tgatttctgt caaaagcagc
      301 tgttctccca gttcttcgcg aatttcgcgg cgtagtgcct cttcaattcg ttcgccatac
      361 tccacgccgc cacccgaaag cgcccattga ccggggaaaa cgccgcgatc gtcggccatt
      421 ttgcacagca gataagcacc atcattttga attaaagggc atacaatagt ccgttgtcgc
      481 acaggggtat tccttataaa taaaaagaaa ccatgagaat acggtaactc agaaatatgt
      541 gaaagtgaca gtaatctctt ttgcgatgat aactgcactt attattattt ttgtgtgtat
      601 cggtaaagtg tgtttatttt ttcggcgttt aattatttaa gaagtattta gttacaataa
      661 gtgactagat cccttttttc gctcattgaa tcctctgtgc caggcagtac aatcccgcag
      721 ctttgggaat tttgattaat tgcttgtggg aaaggggata atgaaaaaaa ttgcgctagc
      781 aggtctggcc ggaatgctgt tggtttctgc atcggtcaat gcaatgagca tcagcggtca
      841 ggcgggtaaa gaatacacca atattggtgt cggttttggg actgaatcga cgggcctggc
      901 gttaagcggt aactggacac ataacgacga cgacggtgac gtcgctggcg tggggctggg
      961 gttgaatctg cctctagggc cgttaatggc gaccgttggc ggaaaaggcg tttacaccaa
     1021 cccgaattac ggcgatgaag gttatgccgc agcggtagga ggtggtttgc agtggaaaat
     1081 tggcaacagc ttccgtttgt ttggcgagta ttactactct ccggattcac tctccagcgg
     1141 tattcaaagt tatgaggaag cgaatgctgg cgcgcgttcc accattatgc gtccagtcag
     1201 tattgaggcg ggttatcgct acctgaattt gtcgggtaaa gatggtaacc gcgacaacgc
     1261 cgtggctgac ggcccgtatg ttggggttaa cgccagtttc tgatcccttc cggcgcggca
     1321 ttctgtcgcg ccattcagtt tcttactacg ccttatctcc ctttgcgtta tagtgattcg
     1381 ataacaaaag caggagataa ccatgttaaa agtggaaatg ttatccaccg gggatgaagt
     1441 gttacacggg caaatcgttg acactaacgc tgcctggctg gccgattttt tctttcatca
     1501 ggggctgcca ttatctcgcc tcaatacggt gggggataac cttgatgact tagtcaccat
     1561 tcttcgcgaa cgtagtcagc acgccgatgt gctgatcgtt aacggcgggc tgggaccgac
     1621 cagcgatgat ttaagcgcac tcgccgctgc gacagcaaaa ggtgaaggcc tggtgctgca
     1681 tgaagcctgg ctcaaagaga tggaacgcta tttccacgaa cgtggacgag taatggcacc
     1741 gagcaaccgt aaacaagcgg agctgcctgc cagtgctgaa tttatcaata acccggtagg
     1801 caccgcctgt ggttttgccg tgcagcttaa tcgttgcctg atgttcttta ctcccggcgt
     1861 accgtcagaa tttaaggtga tggtcgagca cgaaatcctg ccgcgcctgc gcgagcgttt
     1921 ttctttacca cagccgccgg tttgtctgcg tttaactacc tttggtcgtt cggaaagcga
     1981 tctggcacaa agcctggata ctctacaact gccgccgggc gtaacaatgg gctatcgctc
     2041 ttcaatgccg attatcgaac tgaaactcac cggaccggca agcgagcaac aggcgatgga
     2101 aaaactgtgg ctggacgtta aacgtgttgc cggacagagc gtgattttcg aaggcaccga
     2161 aggactgccc gtgcagatca gtcgcgaatt gcaaaaccgc cagttcagcc tgacgttgag
     2221 cgagtaattc accggtggtt tattggcttt gcaactttct cgcgcaggtg ctccattgct
     2281 ggcgtgtgaa gtggttcctt cacaggaaga aaacctggcg caaactgcgc actggattac
     2341 agaacggcgg gccaaccatt ttgccgggct ggcactggct gtttcgggtt tcgagaacga
     2401 acatctcaac tttgcgctag ccacaccaga cggcactttc gctctgcgtg tgcgtttcag
     2461 cactacgcgc tacagcctgg ctatccgtca ggaagtgtgc gcaatgatgg cactgaatat
     2521 gctgcgccgt tggttaaacg gccaggatat cgccagtgag catggctgga ttgaggttat
     2581 tgagtccatg accttatctg tctgattccg ccactctgtg ccggtttact gcaccggcac
     2641 attccccctc cggcaaaact tgtccaacct gttgcactca ataagaaaaa atccgtgcac
     2701 tgattcacaa atttgtgcgt aatttctcca gtccgctgga cgaggagcgg gatttgatgc
     2761 gcttcacaat tttactcccc ctgagcaacg agactgattc cagtatctaa gactggagtc
     2821 cagtatgttg gaatcaagca aagttcccgc cctgacacgg gctattgata ttctcaattt
     2881 gattgcccgc attggcccct gtagtgctgc gacgatcatt gacacattgg gaatccctaa
     2941 aagcacggcc tatttgctgc ttaatgaact caggcgtcag cgttttctta gccttgatca
     3001 ccaggaaaat ttttgtttgt ggaccaggct ggtggagctt tctggtcatg cgttaagcaa
     3061 gatggatctc cgggaactgg ctcgaccgcg tttgacgcaa ctgatggata ccaccgggct
     3121 gttatgtcat ctggggatta ttgataacgg cagcgcttat tacattttaa aagtggagtc
     3181 atcagccact atcagcgtgc gttcccatga aggaaaaagc ctttcgcttt atcgctccgg
     3241 aataggtaaa tgcctgcttt cctggcaacc tgcagcggta cagcaaagta ttatcgaagg
     3301 gctggtctgg gagcaggcga cacccacaac gattactcat ccacagcaac ttcacgaaga
     3361 actggcgcgt attcgccgtc agggctggag ctatgacaac ggcgaagatt accccgatgt
     3421 ccgttgtgtc gctgcgccgg tgtttaacgc caataacgag cttaccgctg cgatctcggt
     3481 ggtcggtacc cgcttacaaa tcaatgaaga ataccgtgat tatctggcgg gcaagtccat
     3541 tgcctgcgcc agggatattt cacgtctgtt gggatggaaa agtccctttg acttacaagc
     3601 ctcataacaa tggagaacat catgacccta ccaaaaatta aacaggttcg cgcctggttt
     3661 actggcggtg cgacagcaga aaaaggcgct ggcggcggtg attatcacga ccagggggcg
     3721 aatcactgga tagacgatca tattgccacc ccgatgagta aataccgcga ttacgagcag
     3781 tcacgccagt catttggcat taacgttctt ggcacgttgg ttgttgaagt cgaagcagaa
     3841 aacggccaga ccggattcgc tgtttcgaca gccggtgaaa tgggctgttt tatcgtcgaa
     3901 aaacacctta accgtttcat tgagggtaaa tgtgtcagtg atatcaaact gatccacgat
     3961 caaatgctca atgccaccct gtattactcc ggctctggtg gcttggtgat gaatacgatt
     4021 tcctgtgtcg atctggctct gtgggatctg ttcggcaaag tggtcgggct tccggtttat
     4081 aaacttttag gcggcgctgt tcgtgatgag attcagttct acgccacagg tgcgcgtccg
     4141 gatctggcaa aagagatggg ctttatcggt ggcaaaatgc cgacgcactg ggggccacat
     4201 gatggcgatg cggggatccg caaagatgcc gctatggtcg cggatatgcg tgaaaaatgc
     4261 ggtgaagatt tctggttaat gctcgactgc tggatgagtc aggacgtgaa ctatgcgacc
     4321 aaactagccc acgcttgcgc gccctataac ctgaaatgga tcgaagagtg cctgccgcca
     4381 cagcagtatg aaggttatcg cgaactgaaa cgcaacgcgc cagtcggaat gatggtcacc
     4441 agcggtgagc accacggcac actgcaatct tttcgtacgc tttcagaaac cggtatcgac
     4501 attatgcagc cggatgttgg ctggtgcggt ggtttaacca cgctggtgga aattgccgca
     4561 atcgccaaat ccagggggca actggtggtg ccgcacggtt cgtctgttta ctctcaccat
     4621 gcggtgatca ccttcaccaa tacgccattc agcgaattcc tgatgaccag cccggattgt
     4681 tcaacgatgc gtccgcagtt tgacccgatt ctgctcaatg agccggttcc ggtgaatggt
     4741 cgtattcata aatcagtgct tgataaaccc ggttttggcg tcgaactcaa tcgtgactgc
     4801 aatttgaaac gcccctacag ccactaatca ctccgggcgt tgctgtgaca acggtaacgc
     4861 ccacatattc taaggagctt tttatgagca ccgctttgct tgacgccgtg gtgaagaaaa
     4921 accgtgtgcg tttaattccg tttatgttgg cgctgtatgt gctggcgttt ctcgaccgtt
     4981 cgaatatcgg ttttgccaaa cagacctacc agattgatac cgggttgagt aatgaagctt
     5041 atgcgctggg agcaggcatt ttctttgtgg tatatgcgtt tctgggtgtt ccggcgaatc
     5101 ttttgatgcg caaactgggg gccagaacct ggattggtac gacaacactg ctgtggggat
     5161 ttctttcggc agccatggca tgggccgata ctgaagcgaa atttctgata gttcgcactc
     5221 tgcttggtgc tgcggaggcc ggatttttcc ctggtatgat ttatctcacc tcgcaatggt
     5281 ttccgcagcg taatcgcgcc agcattatgg ggctgttcta tatgggcgca ccgctggcgt
     5341 taacactggg atcaccgctt tccggcgcgc tgttggagat gcatggattt atggggcatc
     5401 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggtgct ggggtattca
     5461 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa caagaaaaaa
     5521 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
     5581 atgcgctgcg taatggccga gtctggcaac tagcgattat ctacctgacc attcaggtgg
     5641 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
     5701 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
     5761 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
     5821 tactggcggc gggcattggt attggtctgt ccgggctgct ttctccagta atggcgatcg
     5881 tagcgctgtg tgttgcagct atcgggttta ttgccgtgca gccggtgttc tggacgatgc
     5941 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgtttg
     6001 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
     6061 gcgatgcggc aggattactg acactggcag cggtggcggt catcggttcg ctgattattt
     6121 tcactctgcg tgtaaatcgt actgttgcgc agaccgacgt ggcacatcat taaataggtt
     6181 aaggaacacg atgaacgcat tattaaccaa tccctttaaa gaacgtttac gcaagggcga
     6241 agtgcaaatt ggtctgtggt taagctcgac tacttcttat atggcagaaa ttgccgctac
     6301 ttccggttat gactggttgc tgattgacgg ggagcacgcg ccaaacacca ttcaggatct
     6361 ttatcaccag ctacaggcgg tagcgcccta tgccagccaa cccgtgatcc gtccggtgga
     6421 aggcagtaaa tcgctgatta aacaagttct ggatattggc gcgcaaactc tattgatccc
     6481 gatggtcgat actgccgatc aggctcgtca ggtggcgtct gccacgcgct atcctcccta
     6541 cggtgagcgt ggtgttgggg ccagtgtggc acgggctgcg cgctggggac gcattgagaa
     6601 ttacatggcg caagtcaacg attcgctttg tctgttagtg caggtggaaa gtaaaacggc
     6661 actggataac ctggacgaga tcctcgacgt cgaagggatt gatggcgtgt ttattggacc
     6721 agcggatctt tctgcgtcgt tgggctaccc ggataacgcc gggcacccgg aagtgcagcg
     6781 aattattgaa accagcattc ggcggatccg tgctgcgggt aaagcggctg gttttctggc
     6841 tgtggctcct gatatggcgc agcaatgcct ggcgtgggga gcgaactttg tcgctgttgg
     6901 cgttgacacg atgctctaca gcgatgccct ggatcaacga ctggcgatgt ttaaatcagg
     6961 caaaaatggg ccacgcataa aaggtagtta ttgatatcaa aggcccatgg ggatcggctg
     7021 tgggcctgtg ttaattagtg gttattcgct gccagatcgg cttcgcttag ctgggtggcc
     7081 gcgagcacct ggtcacgatc aatgccctgt tccagcatgc gcaaagcaat tttaatggct
     7141 tgttcatgca taccttcctg ccagccaatt tgatgccctt cctgccgtag ccgttcagca
     7201 atagtcatta atctctcctt ttgtagtggt gaacgttcgg caatctcctc aataaaacgg
     7261 gtgaaacggg aggtatcgcc gcattgcagc agataattaa acagtgtttg tagctggctg
     7321 tcattagtga agcctctaac caaaagcgtg gtgatcctgt cgaccatgcc gattaaatcg
     7381 cggtcgcgaa tatgcttttg aatcagttcc agcagagcta tacgccgatg ttgcatgatc
     7441 tcatcgtcag gtacgatggt gatatccacc aacggaaaag cttcggtgta caactgccgg
     7501 gcaagttgcg gatcgtcaaa ctcatccagc cagttaagtg agtacgggta gggcgatgtt
     7561 tcgccatgat aaaacagcaa tggcaccacc agcggaactc tgtcatagcc tttatccagg
     7621 tgacgctgca tggcggcagt ggcatagcgc attagccgaa aagccatatt cttttctgca
     7681 gagctttgat gttcaatcac gcagtagata tagccgtcac cgtcgctggt ttccacggac
     7741 cacaaaacat ccgagtagta agcgcgtaaa cttttttcaa taaaactagt gggttccagg
     7801 cgtaaggttt gcaggttgca aagcttgcgc agtggttctg gtaaatgtat ttcgagaaaa
     7861 tcccgtgcgg tttcgggtgt gaacataaag gttttaaata ccgcatcatg cggggaggag
     7921 gttgttgatt cggtcatggt tgtccgtcac ctgatattta tgatgacgcg acattacggt
     7981 tatcctaggc ctttgacatc tgccagtttc agcctttgcg agacgttttc agaggaattt
     8041 tcactgcgtt gcaacgcata cattgggcac ggaatcgtgt acgcaaaaat gcccggtaac
     8101 cacatgctta ccgggcatca gaaaggagtt taagccagcg cctgggccag tagcgtaatc
     8161 ggatgttcgc agcgaagact tgtggacatc tcaatctgcc atttacaggt ttcgcagtcg
     8221 gtgaccacca gatctgcgcc gctttcttct atctggcgga acagtggtgc gccgatggct
     8281 tgtgaggtgg ggtagttctc ttttttgaaa ccgtaagtac ccgcaatacc gcagcactgg
     8341 gaatccagca ccgttaactc aagccccggg attttacgca acagctccag ggtgtagagc
     8401 gtccagccca ttttttccat atggcacgga gtgtgataaa ccactttcag cggcagcggt
     8461 ttcagcggta acgttttgcc ttcgtccagc ttgcgccaca gccagcgggt tgccagttcg
     8521 atatgatcgc gcaagccttt gttgtcgaca ttcagcactt ccgggtattc gtcgcgcagg
     8581 gcaaatgtac aggttgagga ggtggcaatc accggaatgc cttttactcc cacagcttcg
     8641 cggatcgact ctacattcgt aattgcctgt ttgcgtgctt tatcggtaaa gccgttggcg
     8701 attagcggta cgccgcagca tttttctttg ctgagcagtt gtacaccggt acccattgcg
     8761 ttgagcactt taattaaatc tttacctaac tgcggatggt tgtagttaac gaagcagccg
     8821 tgaaagaaag cgacctggtc tttatattgt gcttgctgag ccgctatgct gcgataccag
     8881 cgacggaacg tgccgaagga gtatttcggt agcgtgcgac gatgatcgat ttttaacgcc
     8941 gcatcaagca gctgccgcac cggtttcagc gaggtagcgg tgttgacgat tggtgcgaac
     9001 ggcgtggaaa cgctacccat caggtcggta tgactcaaca caaaattacg cagcgacggg
     9061 cgcgtggtgt catattttgc ccgcgcgcgc tggataatat cgccaatctt cacatcggac
     9121 gggcaggcga cttcacaacg tttgcagttg atgcaatatt tcagcgcctc gtcatacagt
     9181 gcgccatctt tcaaacgcag acgctcgcca tccggcccgg cttgttttgg ccctggataa
     9241 ccgggattca cccggctcac cgggcaggcg gtggtgcaga cggtgcactt aatgcagttt
     9301 tcgaagctgg tgtcgttcat tgttggcctc ctgcgcgttg ggcaatctgt tgagcggcat
     9361 gtaaagcact gacggcacaa acaccgccgc cgcatccctg ggcgatggga tcaaatccgc
     9421 ccagcaccga accgatggca aacaggttct caatggtttg ccctgcctgc gacgggcgta
     9481 gcgtctcatc agtggttaca ccgaactgct gccacggttg cggcgcaaaa aaatcgccct
     9541 tataccattc accccgcgtg gcggtttgta gcacatcaag gccgagaatc ggctcgcgaa
     9601 tgccgttacg ttccgctacc agtccgccac taaagaaact gccgctggcg agaaccgcga
     9661 aatgtggacg tagcggaata tcggcgtgat tgcgggtcca gatttcattc actacgccat
     9721 ttttacaggt cacttttttc acttcatcgc ccggcatcca cacgccaccc tggcgcacaa
     9781 actggcgctg taactggttt tgcagacgaa tgcccagcac ggaaggcggc agcgttggca
     9841 aaagcatcag tgaacaaagt agtttttcat tcaaccaacg ccacagtttg tcatcggcca
     9901 ggccgaagca ggcaggcatc aggatcattt cgcaggtatt ggcgacagga ataagcgcat
     9961 caagtaacag cggccagttt tcttcattat caaggaaacg ggcgatattc acagcgcgaa
    10021 attcggtggc gttattgcgc aggacatcca gttccggcag ctctatttct gcggtttcaa
    10081 ccgaaagatc cagttcgtgc aacgaagccg ccgcaagatg cgcctgaaaa tccatcaggc
    10141 cgcttatccc gaccacacat attttcttcg cgggcaacgg ccagacgggg acttctggcg
    10201 aacttagcca ggtagagcgc agagtgccga gcggcgtaat ccgctggtga gccagttcta
    10261 cgctgccctg caattgcgca ccgctttcag cgattaatgc ctgcgcctgg caagcgagat
    10321 cgagcacgcg ttgtggcccg agaagagagt aaggatgtgc aggtgcctgc tgacgcaaag
    10381 attccagtcc actgtgaatg tctgccacgg gttgaccatc tggcagatgg ctcagcaaat
    10441 ccagcgaacc ggatgagaaa tgcaacgcgc tttgaccacg cgtgacaatg gcacagcgca
    10501 ggccgtgttt ttgcagttgc aggccacaga gtaatccggc gaggccgccg cccataatga
    10561 cagtatcaaa gcgcatcttt ctgctccttc tccagaccac acagtccctg ataaacccag
    10621 cgggtaaatt cgctttcgcg cagtgcgtct ccccaggcga tgggttgcac gcctttccag
    10681 cgttcgttaa ggaaggtgga aagttgctcg atagattgcg cggacgtcgt gacgttaaac
    10741 cgttgcagca gtccggcagc gcggcaggcg cagagttcgc cctggcaggt gcccatcccc
    10801 acacgggtac gacggcgtaa atccagcagg ctattaacgt ttaaattttc taccgcgtac
    10861 tgcacttcac ccgcagttac cgcttcgcac tcacatacca ggctacggtg cagacggcct
    10921 tcgctcagcc aggcaggcgt gcgatcgcca tgacgataaa ccgcagaacc gcgcagcggg
    10981 gcaggcaggg agatgacttt acgcaaggta acttcagcgg gttcttgtga accaggcagt
    11041 gccagatcgg cagtcgtaca ggggcgcgtg ttgcccagtt tgcggcatac cgcgtcggta
    11101 gcccattcag ccatcagccg ataggtcatc agtttgccac cggtgatggt gataaatccg
    11161 tccagaccat cgcgttcagc atggtcgagc agcacgatgc cacggctgac gttacgtccg
    11221 ctcggatcgt catcgctggc aaccagcggg cgcacgccag aataggcccg caaaatgcgc
    11281 gttttcgcca tcacgggggc cagtttttcc ccttcacgca gcagaatatc aacctcttct
    11341 gccgtcactc gattatcgtc aatctcgttg taatcaatac gtaaagaggt ggtaccaatc
    11401 agcgaaatgg tatcgccagg caccagaata tcggcgtcgg aaggtttacg gcagcggttg
    11461 atcacatgct ggttaatgcg gtgatccatg atcagcagcg atcctttcgc cgggaacatg
    11521 cgaatgcgca gatcggcata ttcggcaatg tgttgccccc agatcccagc ggcattaacc
    11581 acgacaggtg catgaagggc ctgagtttcg ccggtgagat ggttacgtac acgaacaccg
    11641 cacaccgtcg cgccttcacg aatcagcccc gtgacttcat gagcggtaag gataacggca
    11701 ccgtgttctt tggcatccag catgtttgct gcggtcagac gaaatggatc aacggtgcca
    11761 tccggaactt tcaccgcgcc aatcagtgcc gggttaacgg caggttcgat aatgcgcgct
    11821 tgctgcgggt ctatagcttc tgcgctgatc cctgcttctt cgcaggcgcg aataaaagtg
    11881 gcctggaatg agaggtcatc ttccggcagg gtgataaaca ggccgttggt tggttcaacg
    11941 cagtgacgtg caatgcgttt caggatctgg ttttcactaa tgcattcgcg ggccgattcc
    12001 gcatcggtta ccgcatagcg cgcaccgctg tgcagcaggc cgtggttacg cccggtggca
    12061 ccggttgcga tgtcgtggcg ctcaaccaaa atcacgcgca gcccgcgcag ggcacagtcg
    12121 cgggcaatcc cggctcccgt tgcgccgccg ccaatgataa tcacgtcact tgattgcgag
    12181 tcgcgagttt tcattgttat ccctctgaag ttcgtttttt accatttagc catagtaaaa
    12241 acatgaattg tttgatttcg cgcatgttcg ctcataattc gaaagtgaaa cgtgatttca
    12301 tgcgtcattt tgaacatttt gtaaatctta tttaataatg tgtgcggcaa ttcacattta
    12361 atttatgaat gttttcttaa catcgcggca actcaagaaa cggcaggttc tctcactgaa
    12421 tcaggctgtt aatcataaat aagaccacgg gccacggagg ctatcaatgt tgagtatttt
    12481 taaaccagcg ccacacaaag cgcgcttacc tgctgcggag atcgatccga cttatcgtcg
    12541 attgcgctgg caaattttct tggggatatt ctttggctat gcggcttact atttggttcg
    12601 taagaacttt gcgcttgcta tgccttatct ggtcgagcag ggattctctc gcggtgattt
    12661 aggtttcgcg ctttcgggga tctcgatcgc ttatggattt tcgaaattca taatgggttc
    12721 ggtatcagat cgctcgaatc cgcgcgtttt cctccccgca ggtttgattc tggcggcggc
    12781 agtgatgttg tttatgggct ttgtgccatg ggcgacgtcg agcattgcgg tgatgtttgt
    12841 acttttgttc ctctgcggtt ggttccaggg gatggggtgg ccgccgtgtg gtcgtactat
    12901 ggtgcactgg tggtcgcaga aagaacgtgg cggcattgtg tcagtgtgga actgtgcgca
    12961 caacgttggt ggtggtattc cgccgctgct gttcctgctg gggatggcct ggttcaatga
    13021 ctggcatgcg gcgctctata tgcctgcttt ctgcgccatt ctggtggcat tattcgcctt
    13081 tgcgatgatg cgcgataccc cgcaatcctg tggcttgccg ccgatcgaag agtacaaaaa
    13141 tgattatccg gacgactata acgaaaaagc ggaacaggag ctgacggcga agcaaatctt
    13201 catgcagtac gtactgccga acaaactgct gtggtatatc gccatcgcca acgtgttcgt
    13261 ttatctgctg cgttacggca tcctcgactg gtcaccgact tatctgaaag aggttaagca
    13321 tttcgcgctg gataaatcct cctgggccta cttcctttat gaatatgcag gtattccggg
    13381 cactctgctg tgcggctgga tgtcggataa agtcttccgt ggcaaccgtg gggcaaccgg
    13441 cgttttcttt atgacactgg tgaccatcgc gactatcgtt tactggatga acccggcagg
    13501 taacccaacc gtcgatatga tttgtatgat tgttatcggc ttcctgatct acggtcctgt
    13561 gatgctgatc ggtctgcatg cgctggaact ggcaccgaaa aaagcggcag gtacggcagc
    13621 gggctttacc gggctgtttg gttacctggg cggttcggtg gcggcgagcg cgattgttgg
    13681 ctacaccgtg gacttcttcg gctgggatgg cggctttatg gtaatgattg gcggcagcat
    13741 tctggcggtt atcttgttga ttgttgtgat gattggcgaa aaacgtcgcc atgaacaatt
    13801 actacaaaaa cgcaacggag gctaatggca tgaaattgac gctgaaaaac cttagcatgg
    13861 cgatcatgat gagcactata gtcatgggaa gcagtgcaat ggcggcggac agcaacgaaa
    13921 aaatagtcat cgcccatcgc ggtgccagtg gatatttgcc ggagcatacg ctgccagcaa
    13981 aagcgatggc gtatgcgcag ggagcggatt atctggaaca ggatttggtg atgaccaaag
    14041 acgaccatct ggttgttctg catgaccatt atctcgatcg tgttactgat gttgccgatc
    14101 gtttcccgga tcgggcgcgc aaagacggtc gttactacgc gatagatttc acgctggatg
    14161 aaattaagtc gttgaaattt accgaaggtt tcgatattga aaacggtaaa aaagtgcaga
    14221 cttatccggg gcgtttccca atgggtaagt ccgacttccg ggtgcacacc tttgaagaag
    14281 agattgaatt tgttcagggg ttaaatcact ctaccgggaa aaatatcggt atttatccag
    14341 aaatcaaagc gccgtggttc catcatcagg aagggaagga tattgcggca aaaacgctgg
    14401 aagtgctgaa gaaatatggt tacaccggta aagacgataa agtttatttg caatgttttg
    14461 atgctgatga gctgaagcgt attaagaatg agctggaacc caaaatgggc atggagctca
    14521 atctggtaca gctgattgcc tataccgact ggaatgaaac gcagcagaaa cagccggatg
    14581 gaagctgggt taattacaac tacgactgga tgtttaagcc gggtgccatg aaacaggtgg
    14641 cggaatatgc agatggtatt ggtccggatt accatatgtt gattgaggag acatcgcagc
    14701 cgggtaatat caaactcact ggcatggtgc aagatgctca gcagaataaa ctggtagtgc
    14761 atccttatac cgtgcggtca gataaactgc ctgaatacac tcctgatgtg aatcagttat
    14821 atgatgctct gtataacaaa gcgggtgtaa atgggctgtt tactgatttc cctgataagg
    14881 cagtaaaatt tcttaataaa gagtaatgtt actgcaggat ggcgttcatg cgccatcctg
    14941 tctcatatca aatttttcca atcgccacta attgttccat gcacatttgt agtaaaaaca
    15001 gtggactgcc ttctaccggc ttttctttag ccagcaacga aatctgtcga tagaaagttg
    15061 gttcgagagg aacggaacac aattcacctg caatgctttt cagcgttaac tctggttgta
    15121 aggcgatgcc taatccctgt cgaataaaat tcatcgctgt atccgcctga ttaaattcac
    15181 attttatctg ctgggcgcac tattagtggt agtggctggt ggaatctatg cgctgcaaat
    15241 gaagggtaaa agcggtgtca agctagtagt ggcaaaagaa attcattaat acgacacgtt
    15301 tcattaagat tttcctcagg taaccaccta tagtcattgt cgttaataaa aacgacaggc
    15361 gatagggtat ggcagtttca gcaaagtacg acgaatttaa tcactggtgg gcaacagagg
    15421 gcgactgggt tgaagagcct aactatcgcc gtaatggtat gagcggggtg caatgcgtcg
    15481 agcgcaacgg caaaaagctg tatgtaaagc gcatgacgca tcatctgttt cattctgtac
    15541 gttatccgtt cggccgacca acgattgtcc gtgaggttgc ggtaattaaa taactggaac
    15601 gggccggtgt catcgtgccg aagatcgttt ttggtgaagc ggtgaaaatt gagggtgaat
    15661 ggcgagcgtt gctggtgact gaagatatgg cggggttcat cagcattgct gactggtatg
    15721 cccagcatgc agtatcgcct tattctgacg aagtacggca agccatgttg aaagcagtgg
    15781 cgctggcgtt taagaaaatg catagcatta atcgtcagca tggctgttgt tatgttcgcc
    15841 atatctatgt gaaaacagaa ggtaaggcag aagctggttt tcttgacctg gagaaaagtc
    15901 gtcgccgctt gcgtcgcgat aaagcgatta atcatgactt ccgccagctg gaaaaatatc
    15961 tggagccaat ccccaaagcg gactgggagc aggtgaaagc gtattactac gccatgtaat
    16021 cgttgtaggc cagataagac acataagcgt tgtatctggc actacaacca attcacatct
    16081 cgatttcaat atcgcctttt gcccggcaac aacagggcaa aatttccccc ggctgaataa
    16141 aggctaacgg ttcggcaatc cagtcaactt gacctgcaac caggcgtgtg cgacaggagc
    16201 cgcagtaacc ttcgcgacac tggtactcaa ccgccacatt gtgggattcc agcgctgcca
    16261 gaagggaagg gtgttcatcc tggcacagca gttgtgtgcc agtgatgcgc agggtaacgc
    16321 gggccatcag agctggaagt tactcaaatc gtcggtgtcc acttccgagt caatctgccc
    16381 gaccagataa gaactgactt ccacttcctg cggagcaacc tgcacgttat cagacaccag
    16441 ccaagtgttg atccacggga tcgggttgga gcgcgtctgg aacggcagat ccaaaccgac
    16501 tgcctgcata cggatattgg tgatgtattc aacgtactgg cagagaatgt ctttattcag
    16561 accaatcatc gaaccgtcgc ggaacagata atccgcccag tctttctcct gttgagctgc
    16621 ctgaacaaac aggtcatagc actcctgctt acactcttcg gcaatttccg ccatctcagg
    16681 atcgtccgcg ccgctgcgca gcagattcag catatgctgg gtgccggtca ggtgcagggc
    16741 ttcgtcgcgg gcaatcaggc gaataatttt ggcgttgcct tccatcaatt cgcgttctgc
    16801 aaaggcaaag gaacaagcaa agctgacgta gaagcggatc gcttccagcg cgttaacgct
    16861 catcaggcaa agatacagtt ttttcttcag ctcgcgcagg ctaacggtta cggttttacc
    16921 gttaacggtg tgggtgcctt cgcccagaag gtgccagtag ctggtcattt cgatcagttc
    16981 atcgtaatag ctggagatcc cttccgcacg tttctggatc tgctcgttgg tgacgatatc
    17041 gtcaaacaca acagacggat cgttaacgat attacgaatg atatgagtat aggaacggga
    17101 atgaatcgtt tctgagaacg cccaggtttc gacccaggtt tccagttccg gaatagaaat
    17161 aagcggcaat agcgccacgt tcggactacg accctgaatg gaatccagca gcgtctgata
    17221 tttcaggttg ctgataaaaa tgtgtttttc gtgctccggc agcgcctggt aatcgatacg
    17281 gtcgcgggag acgtcaactt cttccggacg ccagaagaaa gagagctgct tttcgatcag
    17341 cttttcgaag atgtcatatt tttgctgatc gtagcgggcc acgttgaccg gctgaccaaa
    17401 gaacatcggt tctttgagct gatcattttt cgtctgtgaa aaggtggtat atgccatgag
    17461 tgtgtcctgt tgggagttta atgccggata aggcatttta cgccgcatcc tgtgcctgat
    17521 gcgacgctgg cgcgtcttat caggcctaca aatccgagcc gtaggccgga taaggcgttc
    17581 acgctgcatc cggcatctca atatcagatc ttacatgcgc cgctttcgca gccatcgtcc
    17641 tggattgacg gtaccagatc gtcctgcgcg tcttcagcgc cgtcacgggt gttctgataa
    17701 tacagcgttt tgaccccgaa tttgtaggcg gtgagcaggt ctttcagcaa ctgctgcatc
    17761 ggcacttttc ctgacgggaa acgtgacgga tcgtagttgg tgttagcaga gatcgactga
    17821 tcgataaatt tctgcatgat acctaccagt tgcaggtagc catcgttacc cggcatttcc
    17881 cacagcagct cataggcgtc gtgcaggtgc tcgtagtccg gcaccacctg gcgcaaaata
    17941 ccgtctttcg acgctttgat gctgacgtaa ccgcgcggcg gttcaatacc gttagtggcg
    18001 ttagagatct gcgaagaggt ctcggacggc atcagggcag aaagcgtgga gttacgcagg
    18061 ccgtgcgttt tgattgattc acgcaatgct tcccagtcgt aatgcagcgg ctcattagcg
    18121 atggcatcca gatctttctt ataggtatcg atcggcagga tccctttcgc gtaagtggtt
    18181 tcgttaaacc acgggcactc gccttgctct ttagccagct cattagacgc tttcagcagg
    18241 taatactgaa tcgcttcgaa ggttttatgc gtcaggttgt tggcgctgcc gtcggagtag
    18301 cgtttaccgt gcttcgccag gtagtaagcg aagttgatca caccaatacc cagcgtacga
    18361 cgacccatcg ctccacgttt ggcggccggg atcgggtaat cctgataatc cagcagcgcg
    18421 tcaagtgcac gaaccgccag aattgccagc tcttccagtt catccaggtt attaattgcg
    18481 cccaggttga aagcagacag cgtacacagc gcgatttcac cgttctcgtc gttgacgtcg
    18541 ttcagcggtt tggtcggcag ggctatctcc aggcacaggt tagactgacg cactggcgcg
    18601 atggccggat caaacgggct atgggtatta cagtggtcaa cgttctgaat atagatacga
    18661 ccggtagacg cacgttcctg catcatcagc gagaacagct caacggcttt cacacgctgc
    18721 ttgcggatgc tgtcgtcttt ctcatatttg gtatacagac gttcaaactc ttcctgatcg
    18781 gcgaagaacg cgtcgtacag ccccggtacg tcggacgggc tgaacagggt gatatcttca
    18841 cctttcagca gacgggtata catcagtttg ttgatttgta ccccgtagtc catatgacgc
    18901 acgcggttgc cttccacacc acggttgttt ttcaacacca gcaggctttc cacttccaga
    18961 tgccacatcg ggtagaacag cgttgccgca ccgccgcgca caccgccctg agagcaggat
    19021 ttcaccgctg tctggaaatg tttgtagaac ggaatgcagc cggtatggaa cgcttcacca
    19081 ccgcgaatcg ggctacccag cgcacgaata cgcccggcgt tgatgccgat cccggcacgc
    19141 tgggaaacgt atttaacaat cgcgctggag gtggcgttga tggaatccag gctgtcaccg
    19201 cactcgatca gtacgcagga gctgaactga cgagtcgggg tacgcacgcc ggacatgatt
    19261 ggcgtcggca gcgaaatttt aaatgtggaa accgcgtcgt aaaaacgctt cacatattgc
    19321 aggcgcgttt cacgcgggta gttcgagaac aggcacgcgg caaccagaat ataaaggaac
    19381 tgggcgctct catagatttc gccggtcacg cggttctgta ccagatattt gccttccagc
    19441 tgcttaacgg cagcataaga gaaggtcata tcacggtcgt gatcgataaa cgtgtccatc
    19501 tgcttgaact cttcttccgt gtagtcttcc agcagatgat tatcgtattt gcccatctcg
    19561 accattttca ccacgtggtc gtacagcgca ggcggctcaa actggccgta ggctttttta
    19621 cgcaggtgga agatcgccag gcgcgcggcg agatactgat aatccggcgc atcacgggag
    19681 atcaggtctg cggcagcctt gataatggtt tcgtggatgt cagaggtctt gataccgtca
    19741 taaaactgaa tgtgggagcg cagctcgacc tgggaaatcg aaacgttatg cagtccttct
    19801 gccgcccaat ccagaacgcg atggattttg tcgagattga tgcgctctgt gctaccgtcg
    19861 cgctttgtca ccagcagatt ctgattcatg tatgtcgtac ctgtttttgg aattctttcc
    19921 gcaatagttt catgcagact acctgtagta cgggaagctg cttagaacac tatatatagg
    19981 gggtatgttt gggattcact gcaagatagt gtgaaaatgg ccctcttgca agtgcataac
    20041 tttgtggata actcaggaag gaaaaagtgg ctttcgcgca ccttaggtca gacaaggtgt
    20101 ccgggaaagt caatgggaag agaaaaattt gttaaaaata actgttcggc gatttcttga
    20161 acggtagaat aggttggata aattgatata gatggtttat taatcttttt gctaatgatt
    20221 gcaaaaattg atctaagcac gagtcatcac ataatctgat gtatcagttg tggtcaatat
    20281 ataagcgaat gtaagcgatt gttgatgtcg aaatggttgt tttctattcg gttattgtta
    20341 aatgcgaagc cattttgtta atttacctta tattataaaa aaattattca ttactttaat
    20401 ttagtttaaa tataactggt atcccttaag gttattaact tgaattatga cgcactgata
    20461 ttattcatca aataataaca aaatagccat tgcatcgggt tggacaattt acaaaagaaa
    20521 gataacccat attgtataat gcgtttaaat atgccgtctt atataaaatg atggtcagat
    20581 taatatgcgg atta
//
LOCUS       DAJVOY010000063        20580 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVOY010000063 DAJVOY010000000
VERSION     DAJVOY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.063"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..4392)
                     /gene="mukB"
                     /locus_tag="OQL90_002003"
     CDS             complement(<1..4392)
                     /gene="mukB"
                     /locus_tag="OQL90_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1368784.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGL"
     gene            complement(4392..5096)
                     /gene="mukE"
                     /locus_tag="OQL90_002004"
     CDS             complement(4392..5096)
                     /gene="mukE"
                     /locus_tag="OQL90_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1368785.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5077..6399)
                     /gene="mukF"
                     /locus_tag="OQL90_002005"
     CDS             complement(5077..6399)
                     /gene="mukF"
                     /locus_tag="OQL90_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1368786.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6396..7181)
                     /gene="cmoM"
                     /locus_tag="OQL90_002006"
     CDS             complement(6396..7181)
                     /gene="cmoM"
                     /locus_tag="OQL90_002006"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1368787.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7317..8096
                     /gene="elyC"
                     /locus_tag="OQL90_002007"
     CDS             7317..8096
                     /gene="elyC"
                     /locus_tag="OQL90_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1368788.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8073..8966)
                     /locus_tag="OQL90_002008"
     CDS             complement(8073..8966)
                     /locus_tag="OQL90_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1368789.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQEHFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9120..9866)
                     /gene="kdsB"
                     /locus_tag="OQL90_002009"
     CDS             complement(9120..9866)
                     /gene="kdsB"
                     /locus_tag="OQL90_002009"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1368790.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9863..10045)
                     /gene="ycaR"
                     /locus_tag="OQL90_002010"
     CDS             complement(9863..10045)
                     /gene="ycaR"
                     /locus_tag="OQL90_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1368791.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10097..11329)
                     /locus_tag="OQL90_002011"
     CDS             complement(10097..11329)
                     /locus_tag="OQL90_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1368792.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLALEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11366..12352)
                     /gene="lpxK"
                     /locus_tag="OQL90_002012"
     CDS             complement(11366..12352)
                     /gene="lpxK"
                     /locus_tag="OQL90_002012"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1368793.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12349..14097)
                     /gene="msbA"
                     /locus_tag="OQL90_002013"
     CDS             complement(12349..14097)
                     /gene="msbA"
                     /locus_tag="OQL90_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1368794.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14134..16406)
                     /locus_tag="OQL90_002014"
                     /pseudo
     CDS             complement(14134..16406)
                     /locus_tag="OQL90_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16613..16897)
                     /gene="ihfB"
                     /locus_tag="OQL90_002015"
     CDS             complement(16613..16897)
                     /gene="ihfB"
                     /locus_tag="OQL90_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1368795.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17057..18730)
                     /gene="rpsA"
                     /locus_tag="OQL90_002016"
     CDS             complement(17057..18730)
                     /gene="rpsA"
                     /locus_tag="OQL90_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1368796.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18841..19524)
                     /gene="cmk"
                     /locus_tag="OQL90_002017"
     CDS             complement(18841..19524)
                     /gene="cmk"
                     /locus_tag="OQL90_002017"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1368797.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19697..20460)
                     /gene="ycaL"
                     /locus_tag="OQL90_002018"
                     /pseudo
     CDS             complement(19697..20460)
                     /gene="ycaL"
                     /locus_tag="OQL90_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
BASE COUNT         4957 a         5695 c         4996 g         4932 t
ORIGIN      
        1 caggccgacg acatgaacat gttcggtatt ctggaagact ttacgcacca gtttataggt
       61 ggtgcctttc tccgggctga tattttccgg cgctgcgatg atgagctgca tttgcaaacg
      121 ctcacacaat tcaaacagcg tggcgataga acgagcatcc agtcgcgctg cttcatcgag
      181 gaacagcaga cggcaaggag agatatcttt accgcgcagg cggcgagatt catcttccca
      241 gctttgtacc accatcacca gaatcgacat accggtacca atcgcctcac cggtcgacaa
      301 tgcaccagac tctgcgcgca accaaccatc ggaaccacgg ttaacctcaa cttccatttc
      361 cagatagttg cggtaatcca gcagttcttc gccaatggtc tgcggcgtgc gctgccccat
      421 atcaatctgc ggattaagac gctgatacaa tttcgccaac gcttccgaga aggtcagacg
      481 gttgctgtta aacagatcct gatgctgctc gtgctgttca gagagcacat ccagcagcat
      541 ggcgtgcgtt tcacgcacgt tcacgttgag gcgcacgctg ttcacctgac caaacgatac
      601 gttctgcaac ccctggttga gcatacggat acggttctgc tcgcgctgaa tggttttgcg
      661 aatgatgttc gccacgctgc gggaactgat caccagtttc tgttcacggg aggttaattc
      721 ttcggtcaga cggctaagtt cgatctccat ctgttcgata gcttccaccg gatcatcggt
      781 acgaataata tcctgacgaa tacgttcacg cagatgctga taagccgcca cgaagaactg
      841 aattttacgc tccggacgtt tcggatcttc cgacatgcgc agcacgtcgc gcagatgttc
      901 gttatccgcc accgccagac gcagcgcacc caatgcctta tccgacatag agcgcaaatc
      961 atcagcggag agataagcca gctcacgacg gtgtaagcgg cgctcaacgc cgttatcttt
     1021 caccatgcgc atcaccgcac accagcccgc tttggcggtc actacctgct cgcgcatctc
     1081 aaagtaatcc cgctccagct tgcgcagttt gcgggtcagg ttgtccatct ccgcttcgca
     1141 gaaggtaagc gctttttcca gttgattgcg gcgtgaacgg ttattgctca gttgcgcatg
     1201 cagctcgtca cggcgaatac gcgcccgctc ttctgccccg ctatcagcac acacgccgat
     1261 atcctgcaat tcacgttgca gatcgttgag tagctctttt ttggtgtcgt aagaactttt
     1321 cagcgaagcc agcacctggt tgtactgact caactgcgct gcgtgaccgc gcaacgcttc
     1381 gcgagcgcgg gtacgctccg cttccgcctg ttccagacgt tcacgcagtt tttcgttgag
     1441 atcgctgtta ccgctaagca tttctgccga gtcagagtag ctaaagtgcg cacgacgctg
     1501 caccacttcc gtcagggcaa acgcctgctg acgggcatcg cgctgcatct gttgagagta
     1561 cgcgtaatct tcttttaact gttcgaactg ttccgggtcg ctctgcaata ccgaaacgat
     1621 cggttccagt ttcgccagtt gattgccaaa ctgctgaaca aaacgcgcgg cttcctgggc
     1681 ttcatccaga cgttcgcgga tttcatcgac gcgatccgcc aggctatcat cagccaacag
     1741 gttgagacgc ggcagaatgc ggttcagcgc cgtaacgccc tctttcgcct gctcaaactg
     1801 aatacgctgc tgctggttat cattttcatg attacttaac gcccgctcca gttcgacgcg
     1861 acggctgttc agttgacgga tttctgcttc cgggtcagac tcaaacacaa ccgccagatg
     1921 actgccgata aagcggctga acgcctgatg cagacgctga gttttctgta catcaaagga
     1981 gagcgtggcg aagcgttcgg aaagcacttc acgctcggca tggaggcttt caatacggct
     2041 ttcacgcgca gcacgaccaa acagcggcac ttccgggaaa cgtgaataac gccactgacg
     2101 atcggcgatt ttcaccacta ccgctttttc cagctcatca acgctgaaca cgctgtcatc
     2161 gaatgactgc ggatctcctt cgatcagata gagatcttcc gggcaatcgg tcaagccatc
     2221 taggtgttca gttacctgtg acagatctgg caccacgatg gcgtggcgtg acgggccata
     2281 cagcgctgag aagtacggcg catcttccag gctaacgtcg tcataaattt ctgacagcag
     2341 cacaccacca aaacgctccg ccagcgcgtt cagacgctga tcttcagagc cgccaggctg
     2401 gcttaaacgt tcgatctctt catcgacggc gtttttgcgc gcgcccactt catcgcgttc
     2461 aacaatcgcc tctcgctcac gctccagcaa ctgttgcaga tattcggtga cgtcctggct
     2521 ggaggtaaac tcttcgccgc actgttcgct caactggttg agactgtttt gcgctgccag
     2581 ccaaaccggc gcacgctgca tcaaactctg aatgcgagac tgcagctgtt cctgctcctg
     2641 gcgcagtgcc atgcgctctt cacgggcgtt agacacgcta tcggaaagag aggcaatgcg
     2701 tgcttccagt tcctgatgca gggcttccag ttcgtcgata tcaaaattct tgccctgacg
     2761 tttgcagaaa tctgccagca gacgctcagc ttcttgctgc tcgcgtagac gctgttccag
     2821 ttcgcttaat cgcatccgca gcggctgaac ctgctctgcc aggtgacgct gatcgacccc
     2881 ttcgcgcaat agttcgcgag cgacatccca cgcctcgtta cgcgccagtg ggccgttgat
     2941 tgccaccacc agctgataag cctgctcaaa ctggctgtgc gcggtttgcg ccatgctcat
     3001 tttctgctca agagagagca ttttttcagt cgcttccagc tctttcgcct ggaaggtttc
     3061 cagccattcg gcggcgctgt cggcggttaa gtccggcaga tggcacagtt ctttggcacg
     3121 attaagcgca gcaatcgcct ggttatactg gatcgcgcgc gtttgctgga cgtccagcgc
     3181 ctgctggtag tcagcaagct ggcttttcag ctcgtccact tccagttcgg cagcttccgc
     3241 gcgagcctcg ttctcttcct ggcgttcgat ggcttctgcc accacttcgt tttgctcttc
     3301 cagacggatc tgcagctcat cgagatccgc ttcgtagcgt tcgatcttct cctgctgacg
     3361 cagtgcggtt tgcaccaggt tcaggtgatc gctggccgcc tgataatccg cttccagatc
     3421 gccttcggca ccgttgtgct ctgccagctc acgcgccata tcgacgtgtt tgtactgctc
     3481 agccgccagt tgctgacgcg aagtatgtaa ctcgcgacga aactccaggg ctttgtcgag
     3541 atggacacgg cgctcgttgg cgtgacgcat gtagtccgcc gccacgtagt tggtagcttc
     3601 gctgatcaga tgcttaaaca ggtcacggtc cgactgggtg acacgaatcg cttccagcgt
     3661 catgcggttt tcacgcagcg ccgcttccat gtcctggaac gctttacgca cgccgctgtt
     3721 ttccggcaac agatagtcgc gcagagaacg ggtaatggca ctggagatcc cgccatacag
     3781 cgaagcttcg atcagacgat agaatttgct acggtcagat gcggagcgca gacgacgcgc
     3841 gatgatgccc agatcgaaca tcagcgagtg gtaatcggta atggagttga actgtttaaa
     3901 ctgcacgcct tccatcgcct cgagcttgtc tttcagctcg ttaagcggca gcacgcgcgc
     3961 ctggcgttcg ttcagggttt cggtcaccag ctgtgtcggc tgcaccgaca tcggcagtcc
     4021 ctgaatggca aatggtttga tatccacttt acgatcgcgt ccggcaacct gttgcagacg
     4081 cacaccgacc accacgcgct ggtggcgcga gttaatggtg tcgagcatcg aataacagac
     4141 acccgctttc agcttaccgt gcagaccttt atcgcgcgaa ccgctggtgg ccccggcttc
     4201 cgtagtgtta cggaaatgca gcagggtcag gtcggggatc agcgccgtaa cgaacgccgc
     4261 catggtggtg gatttacccg ccccgttacc gccagaaagc gtcgtgacca gctcgtcaag
     4321 gtcaaaagtt cgggcaaaaa agccgttcca gttaatcagc gtcagtgagc gaaatttacc
     4381 gcgttcaatc attattcttc ctctccgcta tctggctgat tctcttcggt ttcatcgttg
     4441 agttgcagat gattttcaat cggcattgct tcgccatcac gaatcaggcg acgctgtgct
     4501 tcacggggat cgtcgccagc acgcacatcg gctccgaagc ggaacaccga ttcggtaatg
     4561 cgaaacttgc tgctgtcgtg gcccataaac cacaccatgc ctaaacgacg cagacggttg
     4621 agcgaagaac gtactttctc ctgcaacttc tgacggtcaa cgtctgaacc ggttgaacgg
     4681 ttgttcacca gtttcagcag ttttgcttca tcggccaggg tgagcagttc gtcgtacagt
     4741 tcctgctggg tgaaaatccc ctcattcgcc agccgttccg ggctgagata gagataacag
     4801 aggattttcc cgaccatcat atccagttcc gacaagacgg aacgcgggat aagagtggtg
     4861 gaacgtgggc gtaaatagaa gaacccttcc ggtgcgcgaa taagctcaac gttataacgc
     4921 gcgtaaaact cttccagata ttcctgaaaa tccatcagga atgcatgatt atccagttcg
     4981 tcgaggccaa tatggcggcc tgaacgtaag gcgctgtcca gcgccggaaa taacggattc
     5041 gccagcgcct gcgccagctt aaccggcatc acttgttcaa tatttgtcga tgacatgcgc
     5101 ctgtaccttg gctccgtaat cattaatcgg ctgccatttc gctggcagtc cggtgaaatc
     5161 tgcttgcgct acgccaagac gtaccgcctg atcaataaca atacgcgcaa cgtcaaagtg
     5221 acgtgcacgc ggatactgtg acagatattc gcgtaccacc agaccaagat ccagcggcac
     5281 ttgtctggtt ttgtacacgg caagttgttc ttcgatgatc gccgccagct gttcgcggat
     5341 ctcgttaaac tcttcgtatt ccagatcctc aggaagttcc ccagtcactt cttcatcgcg
     5401 cagtgccatc tcttcgtcac gcatatccag cagacgatcg gcattggcat aagttagcgc
     5461 ccacggctca tcaaaatagg tttgtaccga ctgacgtaac cgctgagcaa agacgcggtt
     5521 tttatccata tcgatcgcgg tacgaataaa tttgtgtacg tggcggtcgt agccaatcca
     5581 caagtcgatg gattgctggc cccaactgat aatacgatcg agtttgctct gcagatcgaa
     5641 caccagacga tcgacgaaat gcagatcgtc atgggtcatc gtcgcatcct gaatgcgcaa
     5701 cagattagcc tgcaatttgt cgcctgccgc ttccagcgta tcctgcaatt cacgcagcgt
     5761 tccggaagtt tccgaaagca acaattcaca gctggaaatc gccgcccgcc agtctttgtt
     5821 cagcaactgg gcgatatcgt ccttcacctg ctgctgctgt tcgtccatca gacgttgcgt
     5881 caggtcgata ctgtcgaaaa tttctgctac cgaatatttc agtggcgcat agacattacg
     5941 gtgccagtga aattcatcac cgccctcttc ggcggcatct gctgcgcgtt tgagctcacc
     6001 cgccacaatc gacaactgca tagaaagacg cagcgtagaa aactcgcgct gacggatgta
     6061 gtagtcagta atgccgatgc cgagcggcgt cagacggtaa attgcgttcc cttccgcctg
     6121 ctcgctggta aagcggttca gcagacgttg acgcaccatg tcgttgatcg cgttattggc
     6181 gcgcacgccg atggtttcgc tggtttgctc aaacgcatca ctcacatggc ggaatgcatc
     6241 caccagctcg ccttcactca tctcaccatc cagacgctcg ccgttcagcg tggcaaccgc
     6301 cagcagaaaa gagagtcggt ctacctgcag cgagatggag aagtcatttt ttctggccca
     6361 ggcaaccagt tcggggactg tctgggaaaa ttcactcata ctttatcctt gctctgcggt
     6421 ttgcgcgcgg tgacatgaat ataacgcccc agggtaatat acggttcctg acggcaataa
     6481 cgcgtttcta attcaagtaa tgcttcatag cagtcgcgct gctggtgctt ctcgcgcaga
     6541 taatcatgaa acacgcgaac gcctgtctta cccataattt gccaaccagc ttcttccagc
     6601 cacagataaa cctgcgccgg gtcgcgtgga taatctggcg aaagcgtccg ttttttcttt
     6661 ttcggcattc ccgcctgcac gtaatcaaaa ttcccggcga ccatgttatg catcaacaaa
     6721 ccatgcgcat tgtagaacat taacgacaac acgccgcctg gacgcaacac tgaccacagg
     6781 gtctgcaata cactgcgggg atcggccacc cactcgagca ccgcatggaa caatatcaga
     6841 tcaacgggcg tttccaaatg cgaagcaaca tcctgagcgg cgcaatgtat aaattgcatg
     6901 ttgtcgctca caccttttgc ttctgccgcc tgttttgcgc ggtcgatcat ctgcgcagaa
     6961 agatcgcata aaatgacctg atgcccacgc tcggccattt tgattgcggt ctgcccttct
     7021 ccaccgccag catccagcac acgcagtttt tgcgggccca tttccgccag cacgcgatcg
     7081 agatcctgcc acagaatagc ctgtcgaagc tgccctttgg tggtgccgta aatgttacgg
     7141 gaaaactttt ccgcaatatc atcaaaattg cgatcctgca ttgctttctc caccggttgg
     7201 ctaagacgtt ttctgataaa gacgctattt tgtcacagcc acggcgagaa tgaacccgtc
     7261 tggtgtaata tcgctggcga tcccttgcta tatggttaaa aaaggaacca gaaaggatgc
     7321 tttttacact gaaaaaagtg attggcaata tgttgctgcc acttccgctg atgttgttaa
     7381 ttattggcgc tggcctggcg ctgctgtggt ttagccgttt tcaaaaaacc gcgaagattt
     7441 ttattagcat tgggtggctg gcactcttgt tattgagttt gcaaccggta gccgaccgcc
     7501 tgctgcgtcc catcgaaagc acctatccga catggaataa ttcgcaaaaa gtggactata
     7561 tcgtggtgct cggaggtggt tatacctgga acccgcagtg ggcaccgagc tctaatttaa
     7621 tcaataacag tctgccacgc ctgaatgaag gtattcgctt atggcgcgaa aatccgggat
     7681 caaaactgat cttcacggga ggcgtagcaa aaaccaatac ggtgagtaca gcggaagtag
     7741 gtgccagagt tgcgcaatcg ctgggcgtgc cgcgcgagca aattatcacc ctggatttgc
     7801 caaaagatac cgaagaagaa gctgcggcag tgaaacaggc gattggtgac gcccctttcc
     7861 tgctggtgac ctccgcatca catctgccgc gggcgatgat cttttttcag caggaagggt
     7921 taaatcctct accagccccg gcgaatcagt tggccatcga ctcgccgctt aatccgtggg
     7981 aacgggcaat cccttctccg gtctggctga tgcatagcga tcgtgttggc tatgaaacgc
     8041 ttggccgcat ctggcagtgg ctgaaaggtc cgtcaggcga gccacggcaa gagtgatttt
     8101 gatgccagat cgaagttgcg ccgactaaat cgtccggtat taaccagttg cgcaacttca
     8161 tcccataaca catacaacca acgccgccag atgaacgact ccgccactgg cgcgcgttgc
     8221 aggtaactcc agagcaaatc ttccgccaga gaattatcca ttagtcggaa cagttcgtat
     8281 tctcgcggtg cccaaagcat tagtcccggc ccgaccatcg ccagtaactg atcgctgcgc
     8341 gaatctttca gcatgctgcg taaacagaag ttaccgtgaa tcaacacaca attgtcgtta
     8401 aagccttcaa ataatgccgg gagacattcg cgagtgcgaa acaggatccg cttatcctgc
     8461 atcgtcagac cggtgttatt gaactgattg agcgtggtcc atagcacttc aacatgttgc
     8521 cggtaccatg agggccagaa atgttcctga gtattgtcga ccgcgccgac gcaaccgcga
     8581 ctgtcctgac ggtgccaggc cagtaaggct tcaacgattt ggtctttgag ctgttcccag
     8641 cgttctggtg ttcgggctgg tgcctccacc gaaacaccac gcatccgctc cagtagcagg
     8701 acatcagggc cggggtgttc ttcatgcgtc atcacaccat aaatagtcgg catacggaca
     8761 gtcccacttc ttgccagcat ggtggttttc catgccagtt gtcgggcttt tccgggcgta
     8821 ctaaagcttc ttgccattaa cggcattggg tttccctggc tgtcatacaa agcccacaac
     8881 gccgtatccg ctttttcatt gacgcactca atacgactga gtttttcgcc cagtaaatgg
     8941 cttaattcgg cacgcagctg ttccattcag attaccctca taaaagctac tgctttcata
     9001 atgagcgcca atataaaaga tgtcacctga atgagatcaa ttgttgaaaa gaaaaattga
     9061 ggaaaatcgt aacggtacga cactcctccc aaaattggct gaagtgtcgt gaagtgaaat
     9121 tagcgcattt cagcgcgaac gcgctcaaga tcttcagggg tatccacacc tgtgccagga
     9181 acttcctgag caacagcaac atggattttt tcaccgtacc acagaacacg aagctgctct
     9241 aacatttcga tgtgttctaa cggacttgcc tgccagttga cgtaacgacg gataaagcct
     9301 gcacggtagc cataaatgcc aagatgacgc aggaagttat cgccaacggt ttcaaggcct
     9361 tctgcaaaac gatcacgatc ccaaggaatg gtggcgcgtg agaagtacag tgcataccct
     9421 tcagcgtcga gaaccacttt caccgcattc gggttaaacg cttcttccgc attgtggatt
     9481 ggcaccgcca gagtcgccat acccacctga cgctgagcga ggttatcagc aacctgacga
     9541 atgattgtcg cagggatcat cggttcatca ccctgcacat taacgatcac cgtgtcgtcg
     9601 ctgaatgcgc atttttcgac aacttccgcc agacgttctg ttcctgactg atgatcggcg
     9661 cgcgtcatac atacttcacc gccagcggct tcaacggcgc gggcaacatc ctcatgatcg
     9721 gttgccacga tgatgcgctc ggcacctgat tcacgcgcgc gttcaagaac atgaacaatc
     9781 atgggtttgc cgttaatatc aaccaatggt ttaccgggca gacgcgtgga cgcgtagcgc
     9841 gcgggaataa tgaccacaaa actcatgatt tactctcatc agcagtcagc acgcgggctt
     9901 ccgtttccag caacactgga atgccatcgc ggagcgggaa agccaggtta tccagtttgc
     9961 aaataagctc ttgtttttcc tggttatacc aaagttttcc gttgcaaacc gggcaggcaa
    10021 tgatttcaag caaacgatga tccatagttc ctccagatgg atcgggttat gaatgcataa
    10081 atcttatcat aatcatttat gcgacggggt ctatttccca gccagtgcgg caatcagtaa
    10141 agagaccttg cgggcagcgt cctaatgtca cccgcgttgc ctgctgccag ctcgcgaaat
    10201 cagtaatcgc ctgacgtaac cctttttgca gcattgtcgt tggtttaatg ccttcctgta
    10261 accacagaga gataacttca aggatgcctg tctggcgatg cattttggca tccattcgcc
    10321 caactaattg cccacgatgt aataatggca gaacaaaata gccatactgg cgtttcggcg
    10381 ctggggtata gcactccagc cggtagctaa aatcaaaaag ctgctctgcg cgtttgcgat
    10441 cccagacaac aggatcaaaa ggcgaaagta ccgcgctgtg cgttgcagtg agctttccgg
    10501 ccagcgcacg ctcgagtagc ggcagcaaat cagcatgcag ccaaagattg cccaattttt
    10561 caacatgcac agcaatgatt tgctgctgtt cagccctcgc ttcgcgccac gccgccagtg
    10621 ccggacgttt cagccgatag taatctgcca gccactgttc gcggaatatt cccaggctac
    10681 gcgcactgtt atccagcatg atgatttctg cttctgtttg cgaaacgaga tcgcgctcat
    10741 catcccagtc aggcatgaca cggtgggtta aatcataaac gcgctggaag ttgcgccgtt
    10801 caatcaccat cacctttccg gcagtaaata aaccttccag atgccgttta tgcggtttcc
    10861 attcccacca gccgcttgca cctttacgag gatgctcaaa atcggctgaa cgtaccggcc
    10921 ccttatcatg aatatgctga attaactgtg caatttccgc ctcatgttcc tgcatccagg
    10981 cgtctttgta tttccagccc attttttcaa gtgccagcat gcggtggcga ataagacgaa
    11041 agtcgctacg cggcataaag caggcttcat gcgcccagta ttccattaat tcgccacgcg
    11101 ccagagactc atccagccac tgggcaggat aatttcccag acgactgaaa agcaccagat
    11161 atggactacg ggcaacaata ttgatggtat cgatttgcag caaggacatg cgggagatcg
    11221 ttgccggaat atcctccaac gacgctcgac ggcggggttt gtttaacagg ccttgtgcgg
    11281 caaggtgaag attacgcgca tcagcaaggg agaggtgcgg cagcgacatt catgactcca
    11341 tcaatcgaac gctgccgcgg cgtaactagt tgccagaagc cagcgaggtt agttgcgtaa
    11401 gcagtttcgc tggttcatca cctgaaagct gtgcgtctac aggcaaatac caccaatttt
    11461 cttctgcaaa ggcccggcat ttcaccgcat ctttttcagt cattaccagc gtttgcccgg
    11521 cgcttaccaa cgcactgaca tccgcatggt tcaaagactg atgatcggcc agcggtacac
    11581 atttttccgg ttgtacgcca cacatcttca gcgtggcaaa aaagcgcggc ggatgcccaa
    11641 tccccgccat cgccactaca tgttcaagct gagcaacgtc acaacgcgta ccggtacgta
    11701 aattcaccgc ctgacccggc agcagatgca tgggaatttc accgctgcga gggacaccac
    11761 cgttgacgat taccgcatca accgacttta agcgccccgc tcgctcacgc attggccccg
    11821 ccggcaacca ccagccattg ccaaagcgac gcacaccatc aataacgaca atttccacat
    11881 cacgcgccag acggtaatgc tgtaaaccgt cgtcggttac gatgatctgc acatcagggt
    11941 gttgcgccag aatggctttt accgcatcag aacgaacggg agaaaccgca acaggcgcat
    12001 cagtgcgttg ataaatcaac acaggttcat cacccgcctg tgctgttgtg gtatctgccg
    12061 acaataacag cggataagat tcagccttac taccatatcc ccgcgatacg acccccacgc
    12121 gaataccgcg ctgttgcaac tgttccacca gccagacaac gaccggggtt tttccgttgc
    12181 cgcctgcggt gagattacca accacgacaa ccggtacggg ggcacgccag gcgcgcttca
    12241 gttttagttt atagcaaaga cggatcgcgc cactcaccag gccatacaac caggagagtg
    12301 gcagcaatag ccgccacaaa ggggattcac cagaccagat tttttcgatc attggccaaa
    12361 ctgcattttg tgaagttgcg cgtaaacgcc gcggtgctca agcaaatcgt tatgcgtacc
    12421 gcgttccaca atgacaccat cctcgacgac cacgatttcg tctgcctttt caatggtaga
    12481 caagcggtgg gcaatcacca gagaggtacg gtttttctgc aactcatcca gtgccgcctg
    12541 aatcgcacgt tcggattcgg tatccagagc cgaggtagct tcatccagaa tcagaatcgg
    12601 gctatcacgc agcaaggctc gagcgatagc aatacgctga cgctgaccgc cagagagcag
    12661 cacgccgttt tcaccaatca ctgtatcgag accgttatcc atcttattga tgaagtccat
    12721 ggcgtaggcc atacgcgccg cttcttcaat ttgctcacgg ctgtactgtt cagtccgtgc
    12781 gtaagcaatg ttgttagcaa ccgtatcgtt aaacagatgg acattctgcg acaccagagc
    12841 aacctggtta cgtaacgacg ccagggtata ctcgcgcaga tcgtgaccat ccatcaggat
    12901 ttcgccttca tcaatatcgt aaaaacgagt gatcaggctg gcgatggttg atttacctga
    12961 accagagcgt ccaaccagag caaccgtctt ccctgccgga attttcaggt tgatgttacg
    13021 caatgcaggt acgtcacgtc ccggataagt aaaggtgaca ttgcggaatt ccacgtcgcc
    13081 agtcgcacgc tcgatcacgc gcttaccttc gtctttctcc tgctcactgt ccagaatggt
    13141 aaacagcgtc tgacaagccg ccataccgcg ctggaactgg gcgttaacgt tggtcagtga
    13201 tttcagcgga cgcatcagtg caatcattga agagaaaaca acggtaatcg taccagcagt
    13261 caggctatcc atgacacttg ggaagctcgc cgcatacaga acaaacgcca gcgccaaaga
    13321 ggcgatcagc tgaatgatcg gatcagagat ggaagaggct gaaaccattt tcatcccctg
    13381 aagacgcatt cggttgctga ctttatcaaa gcgtttcgtt tccacttcct gaccaccgaa
    13441 aatcaatact tctttgtggc ccttcagcat ttgttcggcg ctggtggtca cctgccccat
    13501 ggtgttctgc atgtttttac tgatgttgcg aaaacgcttc gatactacgc gaatcgcaat
    13561 cgaaacaatc ggtgccagca caatcaaaat gatcgacagt tgccaactgt aatagaacat
    13621 catgatgaac aggccgatga ttgacgcacc ttcacgcaca accgttatca gtgcgccgga
    13681 agaagaagaa gcaacctgtt cggaatcgta ggtaatacgt gacaacagcg tccccgttga
    13741 ctgtttgtca aaaaatgaaa ccggcattcc catcatgtga ccaaacaggc ggcgacgcat
    13801 ggtcattacc acctttcctg atacccagga gatacagtag ctggagacat agctggtgat
    13861 accacgtaaa atcatcagcc cgatcaccac cagcggcatc cacaccagca cggagcgatc
    13921 tgttttacca aagccatcat caagaagtgg cttaaggagc gataacatga aggtatcgct
    13981 ggctgcgttg aggattaacg ctacgcccgc cacgatcaga cccgctttaa aaggcgcaat
    14041 ggttggccac agtcggcgga acgtctgcca cgtagagaga tctttgtcgt tatgcattca
    14101 aaaaaccagc atttgttgaa atagccgcat attctacccg ttatccactg gcacgccaaa
    14161 ccactgatga taccaacgag gtaaaatttg ctctctgagg ctgctaatcc gccagccttg
    14221 cgctgaaaaa ttgaccgtca cttgaccctg atgtggagta tcaagccatt gatatccttg
    14281 ctgttgatag cgatgcttaa ctttattaga gggcaatcgc catgcgttat agcgttatag
    14341 cgcgatgccg atgcgagtgc cacttttcca ttcactcgct gaattaatgg caacgatgat
    14401 gaggtattac tgccatggtg aggtacctga agcaatgttg tctgcacttg ctgccagtaa
    14461 cggcttagca tcttttgttc agcggggact tcaatatcac cggttagaag aatgctattc
    14521 gtcccgtcat aaaccttaac cacacaggaa tggttatttc ctttatcgtt gctagcttgt
    14581 aaaggccagt gcacgctgaa acgcaatcct tgccattgcc acgcttcgcc acgcacacag
    14641 ggctgatgat gttcccagtt taacggactt ctgatccata acatcggcca tgtgtgcaat
    14701 attgagtcca gccctccccg gtgatccaga tgttcatgac tcagaataac gccttccggt
    14761 tcaagattat gccagtggag ccaggggata atcagttgtt gcccactatc cccttcgggc
    14821 caggccagtc ctgtgtcata gagaatcgct ttgccgtttc tggctatcac cattgccagc
    14881 ccttgcccga catcaagcat gtacacctgc cactcgtcag gtcgaggttt ttgccacagc
    14941 ggccagcaca tcagcaagcc tacagccaca cacattgctg gcaacgttcg ccaggcgttt
    15001 aatcgccata ccaccagtaa gaaccatggg gaaaatgata gccattgcca acgttcagca
    15061 atgttgatcc acccttctgg caaactcttt aacccccaga aaagtaaagc caaagaccgg
    15121 tcggcaagaa accataaccc ttgctccagg attaacggcc cgcttaaatg cacaaccatc
    15181 gcggcgagga tcaacggaac cgtgataaat gtcaccaagg gaattgctaa tagatttgca
    15241 ataaacgagg tcagactaat gccatgaaat atgacgattt gcacaggcat aagcaggagt
    15301 gtgattccca gttgcaaatg aatgagggaa acaactgcac gcaataccgg cggcagttgc
    15361 cactcaggac agggaaacca ctggtaccaa aaaatcagtg ccgcgaccgc agcggcagag
    15421 agccataaac tttgcgagag aatggcaaca ggatccatca gcaaaattgc cgccagacaa
    15481 catatccata catcccagcc actccactgt cgcccactta acttaagcat tccccacgta
    15541 gcaagcgcca ccacggtacg caatgcagga ggttgcattc ccgtcaacca ggcataaaaa
    15601 gcagcacagc agattccacc aattaatggc atttgccagt ggatccagcg cccaggcaga
    15661 aaaacttgcc caccgcgaat gagtccggca gccagcaacg ccgcaaaagc gatgtgcaat
    15721 cccgatatcg ccattaaatg cgccgttcca gtatcacgca tgatattttt gatttctttg
    15781 gggacggata accgttcccc catacctaaa ccaagaataa ccgcattcca catatagggt
    15841 tgcagcgttg tttgtagtga cgccagatac tgtgcacgca ggctacaatt cggttcaatg
    15901 acacttgcct gcagaaagcg gccgatgagc ggctgatgct gggcaatggc ataacgctgg
    15961 ctatcaaagc cgccatcatt aagttggcca tgaactgcac gaactttgag tttcattgac
    16021 cattgttgtc cagcacaaac cgcttgcgga agatattcgc catacagcac aagacctggc
    16081 gcagggaata tacgtcgacc ttgtagatga gtaatttgac cgtaatgagt ggtcatgccg
    16141 tcagttgcag tgatctcaac aattgcatcc tgcgtcgcgc cagttaaggt ttctcctgcc
    16201 cacaaaattt gctttgctga taatatgccc caaacgaaaa acagcaacgt cagcgcgata
    16261 taacggacgg ttttaacagg gataaatgcc agtacgcagg cgaagagagt cagaaacgca
    16321 agggttaatg tcccaggcaa ttggggcaaa atcagcaacg gaaaaattcc gcaaattatg
    16381 catacgccga ctgtcgttat tttcatgcac ccctccctgt ttatggaaag tgtgcaactt
    16441 tgttgagcgg ttgtctggtg ggaattttgc gttccccgag gcacattcag aagaatttaa
    16501 cgaaagggtg aaaactgctc aaaactctgc gaggcagctt ccagttcctg attagagaaa
    16561 tgaaaaaagc acccgacagg tgcttttctc gcgttcaagt ttgagtaaaa acttaaccgt
    16621 aaatattggc gcgatcgcgc agttctttac caggtttaaa gtgaggaacg tattttcctt
    16681 ccagttctac tttatcgcca gtcttcggat tacgtccggt acgtggtgcg cggtagtgca
    16741 aagagaaact gccgaaaccg cggatttcaa tacgctcgcc ctgcgcaaga gtcgaggcca
    16801 tatgctccag catctctttt actgcatctt caaccgtctt ggcgggaatg tgcgattgct
    16861 gggtggcaag tctttctatc aattctgact tggtcatgat tcctccggtt ccttaaaggc
    16921 aaattaagcg gctgctgttg ctgcattgat taagggcggc cgtagccgcc cttagtgctt
    16981 gattacagga cgaaacctgc aatctgtcaa gtaaactcaa caaacttcgg aataaaaatc
    17041 ccgaagagtc agagaattac tcgcctttag ctgctttgaa agcttcagcc attgcgttgt
    17101 tggagaagtt tgcatcttcc tgtttgttaa cagttgcgat tgcatctttc tcgtcagctt
    17161 cgtctttcgc acgaacagac aggctgattg cgcggttttt acgatcaacg ccggtgaatt
    17221 tagcttcaac ttcgtcgcca acgctcagaa ccagggtagc gtcttcaacg cggtcacggg
    17281 atgcttcaga agcacgcagg taaccttcaa cgccgtcagc cagttctacg gttgcgcctt
    17341 tagcgtcaac tgcagttact ttaccggtta cgatagcgcc tttcttgttc agagcaaccc
    17401 agttgttgaa cggatcttct gcgagctgtt taacgcccaa ggagatacgt tcacgttctg
    17461 cgtcaacctg cagaacaact gcagcgattt cgtcgccttt tttgtattca cgaactgctt
    17521 cttcgcctgc aacgttccag gagatgtcag acaggtgaac caggccgtcg atgccgccgt
    17581 ctaggccgat gaagataccg aagtcagtga tagacttgat tttaccttca acacggtcgc
    17641 ccttgttgtg ggtttccgcg aactgctgcc acgggttagc tttgcactgt ttcagaccca
    17701 gggagatacg acgacgttct tcgtcgatat ccagaaccat aacttccact acatcgccaa
    17761 cgttaacaac tttggacggg tggatgtttt tgttggtcca atccatttcg gaaacgtgta
    17821 ccaggccttc aacgccttct tcgatttcaa cgaagcagcc gtagtcggtc aggttggtca
    17881 cgcgaccagt cagtttggta ccttccggat aacgtttagc gatagctacc cacggatctt
    17941 cgcccaactg tttcaggccc agggatacac gggtacgttc gcggtcgaac ttcagcactt
    18001 taacagtgat ttcgtcgccc acgttgacga tttcgctcgg atgcttaacg cgtttccagg
    18061 ccatgtcagt gatgtgcagc aggccgtcaa cgccgcccag atcaacgaat gcaccgtagt
    18121 cagtgaggtt cttaacgata cctttaactt ccatgccttc ctgcaggttt tccagcagct
    18181 gatcgcgctc tgcgctgttt tcggattcga taacggcacg acgagaaaca acaacgttgt
    18241 tgcgcttctg atccagcttg attactttaa attcaagctc tttgccttcc aggtgcagag
    18301 tgtcacgcac cggacgaacg tctaccagag aacctggcag gaacgcacga ataccgttca
    18361 gctcaacagt gaagccgccc ttaactttgc cgttgataac accggtaaca gtttcagcat
    18421 cttcgtaagc tttttccagc gtgatccagg cttcgtgacg tttagctttc tcacgggaca
    18481 gcagagtttc accgaagccg tcttctactg cgtccagagc aacgtcaact tcgtcaccta
    18541 cctggatttc cagctcgccc tgggcgtttt tgaactgctc agccgggatg gcggactcag
    18601 atttcagacc agcgtcaacc agtactacgt ctttgtcgat agcaacaaca acgccacgaa
    18661 cgatagaacc cgggcgggtt tcgatttctt ttaaggactc ttcaaagagt tgagcaaaag
    18721 attcagtcat gtttaatctt caggtttata tttaacgtcc acctggctcc atgccggatg
    18781 gggttgttta acatacccgc tgtcattcca ttgcaacggg ggtactgcaa attcggtcgc
    18841 ttatgcgaga gccaatttct ggcgcgcgta ttgtagcgct ttttcaatca cttgctcaat
    18901 gcttaaggtg gtggaatcca acactaaagc atcggctgcc ggaaccagtg gcgctaccgc
    18961 tcggttacga tcgcggtcgt cgcgttcttt gatctcggcc aaaaggcgct caaagttaac
    19021 actaaagccc ttctcctgca actgtagcat gcggcgatgc gcacgttctt ccgaggaggc
    19081 gtcaaggaaa attttcactg gtgcatcagg gaataccacc gttcccatgt cgcggccatc
    19141 ggcaatcaga cctggtaatt cgcgaaacgc gcgttggcga cgcaataatg cttcacgaac
    19201 gcgtgggaat gtcgcgactt gtgaagccgc attcgccact tcctgagtac gaatttcgcc
    19261 gctgacatct tccccttcga ggatcacttc cagattgcca ttggttgaca caaaacgtac
    19321 atccagatgg gatgccagcg gtaccagcgc atcttctgac gcaacatcaa catggtgatg
    19381 taatgccgcc aatgccagta cgcgataaat tgcacccgaa tccagcagat gccattgcaa
    19441 tgcttccgcc atagccttac acaaggtgcc tttccctgca ccgcttgggc catcaatggt
    19501 aataaccggg gcaattgccg tcatctttat ctccttaaaa caggcgtacc gttaacataa
    19561 ccgcgcgcat tatacgcgcc gacgccacaa atcgttaacg ttgtgtgcaa aaaccagttt
    19621 tgtctgtatt cagatgggga tttattgcgc aattatagcg ggctggcaga agaccagccc
    19681 gaaagatgac aatgatttac ttaccagagg cgatacgatc acggatgtgt tgcgcacgct
    19741 ctgttgatgg tgggtgagag tcaaacatgg actgggtgcg accgccatcc agactagcca
    19801 gtttttcaaa gctgccaacc agcccctagg tgctgatgcc acgtttcttc aacagatcaa
    19861 aggagaaatc atctgcttcg gactctttat cacgggagta tttagcatta atagcgcctt
    19921 ctgcgatatc acccaattgt gagcgggaaa gctgggaagc cacaccgctg gtagctgaaa
    19981 tggcatcgcg tgcggcaacg atcgcataag aaactttcat ttcagccagc gagtgaccca
    20041 acgcgacgtg gcccagttca tggcccagaa caccttcaat ttcgttgtca ttcatcatgt
    20101 ccatcaggcc actgtagaca cggacacagc cgttcgccat cgcccatgcg ttgacgtcgc
    20161 tggtcatata aacctatagt tgaccggcgt gccattaata ttgttaccca atgctttggc
    20221 gatttttgcc agacgtttac cgtacttgct ggatttactt gcgacttgat tgccgctgtc
    20281 catttcggca cagccctgat tggcaatcgc ttttgcatcg gcatcgctta atgttgctgc
    20341 tttataggca tttaaaccgg agctgatagc catattggta tcaataccgt gggtattttg
    20401 acaccctgtc agtaatgctg cagaggtcgc aatcgccagc agtaatttag tattcttcat
    20461 gtgaatcatc cgttgattaa tcatatataa acattatgta tcaacaactt acattgacaa
    20521 gcgacgctac cattaatcaa aatgactcac aaggtccgaa aaaaaacgcc gacaatgtgc
//
LOCUS       DAJVOY010000064        20173 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVOY010000064 DAJVOY010000000
VERSION     DAJVOY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.064"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..1009)
                     /locus_tag="OQL90_002019"
     CDS             complement(<1..1009)
                     /locus_tag="OQL90_002019"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCS1368798.1"
                     /translation="MKEKTIIIVGGVQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPWFWSDQYSDNLQFI
                     GD"
     gene            complement(1019..1831)
                     /gene="hcaB"
                     /locus_tag="OQL90_002020"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(1019..1831)
                     /gene="hcaB"
                     /locus_tag="OQL90_002020"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1368799.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(1828..2148)
                     /locus_tag="OQL90_002021"
     CDS             complement(1828..2148)
                     /locus_tag="OQL90_002021"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1368800.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            complement(2148..2666)
                     /locus_tag="OQL90_002022"
     CDS             complement(2148..2666)
                     /locus_tag="OQL90_002022"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1368801.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRPRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(2663..4024)
                     /locus_tag="OQL90_002023"
     CDS             complement(2663..4024)
                     /locus_tag="OQL90_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1368802.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            4160..5050
                     /gene="hcaR"
                     /locus_tag="OQL90_002024"
     CDS             4160..5050
                     /gene="hcaR"
                     /locus_tag="OQL90_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1368803.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            5210..6349
                     /locus_tag="OQL90_002025"
     CDS             5210..6349
                     /locus_tag="OQL90_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1368804.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(6341..7621)
                     /gene="csiE"
                     /locus_tag="OQL90_002026"
     CDS             complement(6341..7621)
                     /gene="csiE"
                     /locus_tag="OQL90_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1368805.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(7812..8666)
                     /locus_tag="OQL90_002027"
     CDS             complement(7812..8666)
                     /locus_tag="OQL90_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1368806.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(8811..9614)
                     /gene="suhB"
                     /locus_tag="OQL90_002028"
     CDS             complement(8811..9614)
                     /gene="suhB"
                     /locus_tag="OQL90_002028"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1368807.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAVEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            9733..10473
                     /gene="trmJ"
                     /locus_tag="OQL90_002029"
     CDS             9733..10473
                     /gene="trmJ"
                     /locus_tag="OQL90_002029"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1368808.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10743..11231
                     /gene="iscR"
                     /locus_tag="OQL90_002030"
     CDS             10743..11231
                     /gene="iscR"
                     /locus_tag="OQL90_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1368809.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            11343..12557
                     /gene="iscS"
                     /locus_tag="OQL90_002031"
     CDS             11343..12557
                     /gene="iscS"
                     /locus_tag="OQL90_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1368810.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            12585..12971
                     /gene="iscU"
                     /locus_tag="OQL90_002032"
     CDS             12585..12971
                     /gene="iscU"
                     /locus_tag="OQL90_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1368811.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            12988..13311
                     /gene="iscA"
                     /locus_tag="OQL90_002033"
     CDS             12988..13311
                     /gene="iscA"
                     /locus_tag="OQL90_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1368812.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            13407..13922
                     /gene="hscB"
                     /locus_tag="OQL90_002034"
     CDS             13407..13922
                     /gene="hscB"
                     /locus_tag="OQL90_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1368813.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            13939..15789
                     /gene="hscA"
                     /locus_tag="OQL90_002035"
     CDS             13939..15789
                     /gene="hscA"
                     /locus_tag="OQL90_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1368814.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            15791..16126
                     /gene="fdx"
                     /locus_tag="OQL90_002036"
     CDS             15791..16126
                     /gene="fdx"
                     /locus_tag="OQL90_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1368815.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            16138..16338
                     /gene="iscX"
                     /locus_tag="OQL90_002037"
     CDS             16138..16338
                     /gene="iscX"
                     /locus_tag="OQL90_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1368816.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            16516..17799
                     /gene="pepB"
                     /locus_tag="OQL90_002038"
     CDS             16516..17799
                     /gene="pepB"
                     /locus_tag="OQL90_002038"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1368817.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            17941..18717
                     /gene="sseB"
                     /locus_tag="OQL90_002039"
     CDS             17941..18717
                     /gene="sseB"
                     /locus_tag="OQL90_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1368818.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(19210..20055)
                     /gene="sseA"
                     /locus_tag="OQL90_002040"
     CDS             complement(19210..20055)
                     /gene="sseA"
                     /locus_tag="OQL90_002040"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS1368819.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPKTFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
BASE COUNT         4808 a         5137 c         5493 g         4735 t
ORIGIN      
        1 tatcgccaat aaactgtaag ttatcactgt actgatcgct ccagaaccac ggcagtagcg
       61 gtagcggtag ccctaacatt gcggcagcgg caatttgcgc ctggttattg gcgttttccc
      121 agctttcaca gcggtgtagt gcaccattat caagacgagt gattgccaca tcgccaccgg
      181 caaagatcgc gggatcgcag gtgcggcaag cctcatcaat gacaatgcca ttggcagtat
      241 caaggttggc ctcgcgagcc agttgctcgt tggcgctgat accaataccg taaatcacca
      301 catcagcctg aagcgtttcc ccactttgca gcgtcagttc tactttttca ccatcgacca
      361 catgttcaat ggcattattg agcagaatgc gcacaccagc ctgctggtgg cgctgtaaaa
      421 gatagcgttg cacgggcggt ggtgcattac ggcccatgac ggttgccgcc agttcaatca
      481 ctgtcacctt acatctgcgc tgcgctgcgc tgcgctgcgt ggcgctggca gccagttcca
      541 gaccaatagt tccggcaccg acaatcacga ctgaccgttc gggctgcaga acttctcgca
      601 gtctggcggc atcaccggca tggcgtaggg taaagcagcg ttctcccagt gcatcaagca
      661 acggcagcgg tcgagctgcc gcgccggttg ctataaaaag ctgatcccag tgccagcttt
      721 cgccgttggt taacactaac tctcgtgtgt cgcgacccag cgttttgatg gttacaccgg
      781 aatgcagatg aacattgttt tcctgccacc agttagcggg taacacctgc tgtaactgcg
      841 gggaatcttc cagcaacatg gatttcgaga gtggaggtcg ttcataagga agatgtcgct
      901 catcggaaaa cagatgcagc tcaccggtga acccttgctg gcgtagcgag gccgcagcca
      961 ttgccgccgc ttgcacgcca ccgacaataa tgatcgtttt ttctttcatc gtgcttcctt
     1021 aaagatccag cccagccgct acgtggcgaa tgccgcgaat cgctaaaccc gcatcagcgt
     1081 tgatcatcac accgcttaat gcgcgattat tgcgccgcga tgccaacatc acatacggcc
     1141 ccgtaaaatc cgccggttgc gggaaaaatt gcagcggtaa aatggcggca attttctccg
     1201 gcgtcagaga ctgcattatc gaggtttcac tttgcccgag cgcctgtggg ccgcgcaggt
     1261 cgctggccat accacacggg ccgacgccat tcacccgcac tttcggtgcc agttcataag
     1321 ccagttggcg aataagtccg gttgcggcat gtttactggc ggtgtacagc gggccaccgc
     1381 cgccaggata ccaggcggca tttgacagtg tgaaaatcat gctgccttca ctggcgatta
     1441 acgccggagc gcaggctttt gcgcccagca ggtaaccgag tacgttgacg ttaaacagct
     1501 cgtggaagcc ggtttcgagc gtctctgcgg gagtattaac cagtgaggca ttgtgatccc
     1561 agatgcctgc attgccgata aaacaatcca gcttgccgga acgagtcagg atctgatcga
     1621 ccgcgcgttg ataatcggca taacaggtca cgttaccttc caccgccaga atatgttcgc
     1681 caaatcgctg acgcagactg gcgacttttg ccgccgacag ttccagcgtg gcaacctgcg
     1741 cgccttcttc gataaatcgc tcgaccagcg ccagccctaa tcccgatccg ccgccggtaa
     1801 taaaaatgga ctcgttatgc agatcgctca tggctgcgcc tccggtaagt cgatgaaaat
     1861 gtcactacct tcaacgtgta ctggataagt ggtgagcgga tcggtggcgg gcaggcataa
     1921 cgctttcccc gttttcaggc aaaaactggc ggcgtgtagc gggcactcca ccgtggcgtc
     1981 atcttccaga tacccttctg acatcgacgc attaccatgg ctgcaacgat cgttaatggc
     2041 ataaaactcg ccgccaacgt tgaacagggc gatgacgggc gaggtatcga tccggagagc
     2101 ctcaccctcc ggcacatccg caacgggaca cgcataaatt cgattcatca gaacagtaca
     2161 ctcaggttat gggaagtgat taccgcttga tccaggacga tatcccgttc cagcaagcgc
     2221 cagttgtcat cttccagacg gcgaactttg tcgaaacgcg ttccaacata gaatgtttca
     2281 tcgcgctctt tttgtgcctg ataaagcaga taatttaccc gcacagcaaa tacgtttggg
     2341 atgtcggttt cgcttatctg gcagttgctg attaagtgac gggggcgtga cggcggctct
     2401 tctgcccagg ccatgcccgt ttccagacgg gcgattcgcc gctccagctg gtctttggtg
     2461 tcattaaaaa tccaggttgt cggtggctgg acgcctttgc ggcggtcgcg tgtttgcgcg
     2521 ttaactgtgg tgcgcatggt gtaacgaatc tcttcgtcga gctgcgccag ccagtcacga
     2581 aatttccagt cgtccagtaa gctggcttcg tgaaagagaa actggctaat gcggagatgt
     2641 aactctagtg aaacttgcgc actcatttca tcacctcctg ctggtaagcg gcggttttat
     2701 cgagcacttc ctgccagctt tcgctactca gaagatcggc ccagcgttgg tacattccac
     2761 gagcggcagt ttctgaaaag atatagttag taatgccagg aatgccgtcg tcgcggcgct
     2821 tttcctgacc aagccccatt tccagacaca gtttgctgtt gcgggcgcgg tggcctttaa
     2881 gcaatttctg gatttcacac cagttctccg agtcatcctg ctcgagaaaa ccagcaggac
     2941 caaaagcacg agtggcgctg ttttcaaaag cggctttaac ttcatcggag gcggctttgt
     3001 cagtaataca gaacgcccac acttcaactt gatcagggcc gcgcggatgc cagacgcgga
     3061 gtgtggcagt gccgtttagc catgaaagcg tggggaatat attgttatga cccgccaggc
     3121 gcagggcgcg aacttcacct aaacgttgtt ctgcttcggc ataggtttcg cggtaatagc
     3181 tggaaaccgc gccatcgacc cagacattag catccggttt ttcagtaaag aagaaaccgc
     3241 taccgtgacc gtcctgacca aattgcagcg catctttggc ggtttcccac accgggcggg
     3301 cggtttgtcc atcaccgaga cgcttatcgc tgccatcatc tttcgcccct aatacctgaa
     3361 cggcagaagc atggctgaac agagcatgat attggtcact ggcgaactgc tctgccgggg
     3421 atttccagtt acagttgatc acccactttt gtacgccgcc gacaatttcg gtgccgcctt
     3481 cgcgacgatc cagcatgcca tccagatacc aggcaatgtc accaaggtaa tcacgcaggc
     3541 ccggtgcgct ggtatcccag ttgccaaaaa tcagcccttt ataactctcc acacaaggaa
     3601 cttcgtttag cccccagtgg gatttacaca acccttgtgg gtaggcgcga ggttccagcg
     3661 gtacatcgat caactcgccg ttaatgccat aagaccagcc gtgatacggg caggtaaagg
     3721 cgcgagtgtt gccgcaatct gcataactca cacgcatggc ccggtggcgg cattggttga
     3781 gaaacgcctt gatgctgccg tctttctgac gcactacgac aaccgcatct tctcccatgt
     3841 aggtgttaaa gaaatcaccg ggttttggga tctggctttc gtgggcgaga aatagccagc
     3901 aacgaccgaa aatacgctca agctccagtt ggtaaatatc cgggtcggta taaatacgcg
     3961 gagtgacccg accattttgg gtgtcaatca gttggtaaat gttcaaatct gagggtgtgg
     4021 tcatattttt taatcctgaa agataagatg tattgacctt ctatttttgt gccataagga
     4081 tgttgctggt tggttgctaa tatgtgaaat agttaatttc taccagctga gacgtttttt
     4141 cgataacaag gggaaggtga tggaactacg gcatttacgc tatttcgtcg cagtggcgca
     4201 ggcactgaac tttacccgtg cggcggaaaa actgcatacc tcacagcctt cgttaagcag
     4261 ccagatccgc gatcttgaaa actgtgtcgg tgttccgtta ctggtgaggg ataagcgcaa
     4321 agtcgcgttg acggcggcgg gagaatgttt tctccaggac gcgctggcaa tcctcgaaca
     4381 agcggaaaat gccaaattac gggcgcggaa aattgttcag gaagacagac aattaaccat
     4441 tggctttgtg ccatcggcgg aagtgaattt actgccaaaa gtattaccga tgtttcgtct
     4501 cagacagcca gacaccttga ttgagctggt gagtttaatc accacgcaac aggaggaaaa
     4561 aattcgccgt ggtgaactcg acgtcggctt aatgcgccat cctgtttata gcccggagat
     4621 tgattatctg gagctttttg acgaaccatt agtggttgtg ttaccggttg atcacccttt
     4681 agcacatgaa aaagagatca ccgccgcgca actggatggc gtgaatttcg tcagtaccga
     4741 tccggcgtat tccggttcgc ttgcgccgat cgttaaagcg tggtttgcgc aagaaaacag
     4801 ccagccaaat atcgtccagg tggcaacgaa tattctggtg accatgaatc tggtgggcat
     4861 ggggctgggc gtcactttga tacccggtta tatgaataat tttaataccg gacaggttgt
     4921 ttttcgtccc attgccggaa atgtgccttc cattgcttta ctgatggcgt ggaagaaagg
     4981 ggagatgaag ccggcattac gcgatttcat cgccattgtg caggaacgtt tggcaagcgt
     5041 aacggcataa ttacgcatta tttttgcaac atgcgtcgcg gatcgcagtt ttttagtgct
     5101 tgtgaactgg cgttacagac atcgggaagc aggctcgaat attcttattt ttcaacttgc
     5161 accaacattc gattaacccc agactaacgc ctcctgacgg gagggactca tggttttgca
     5221 atccacgcgc tggttggcgc tcggctattt cacatacttt tttagttacg gcatttttct
     5281 acctttctgg agcgtctggc ttaaagggat tggtttaacg ccagaaacca tcggcctgtt
     5341 attgggggca ggtctggttg cccgttttct cgggagtttg ctcatcgcgc cccgcgtcag
     5401 cgatccttct cgcctgattt ccgccttgcg cgtgctggca atgatgacac ttctctttgc
     5461 tgtcgccttc tgggcggggg cgcacgtagc gtggctgatg ctggtgatga ttggctttaa
     5521 cctctttttc tcaccgctgg taccgttgac cgatgcactg gcgaatacgt ggcaaaagca
     5581 gttcccgctt gattacggca aagtgcgact gtggggctcg gtggcgtttg tcattggctc
     5641 ggcgctgacg ggcaaactgg tcactatgtt tgattatcag gtgatcctcg cgctgttaac
     5701 attgggcgtg gcatccatgc tgctcggctt tctcatccgt ccgacgattc agccacaagg
     5761 ggcaagccgc cagcaggaga gcaccggttg gtcagcgtgg ttggcgctgg ttcttcagaa
     5821 ctggcgcttt ctggcctgcg tttgtttatt gcagggggca catgcggcct attacggttt
     5881 tagcgccatt tactggcagg cagctggcta ctcggcctcg gcggtggggt atttgtggtc
     5941 gctgggcgtg gtggcggaag tcattatctt tgcgctgagt aataaacttt tccgccgttg
     6001 tagtgcacgc gatatgctgt tgatctcggc gatttgcggc gtagtgcgct ggggcattat
     6061 gggagcaact acggcgttgc cgtggttgat agtggtgcaa attctgcatt gcggcacctt
     6121 cacggtctgc cacctggccg ccatgcgcta tattgccgct cgccagggta gcgaagtcat
     6181 ccgtttacag gcggtttact ctgccgtcgc gatgggcggc agtatagcta tcatgaccgt
     6241 tttcgccggt ttcctgtatc aatatctggg ccacggcgtg ttctgggtaa tggcgctggt
     6301 ggcgcttccg gcaatgtttt tgcgcccgaa agttgttccc tcatgctgat tcgagcattt
     6361 tgcggatctg ctcctgttga tgtgttgtca gtgtcaggtc cgtatagatc agcggtggtg
     6421 aaaagagcgg taacggcatg gtataaggcg caataatcag tgccgcgccg cgcggagcgc
     6481 ctgtctgcaa aaatgccttt accgacatat gtttaatgtt gagcggcagt aacgttagtt
     6541 cgcgtaactg ctgctcaatc tgcgcttctc gctcgctatc attcccggta agaagaataa
     6601 tctgtttttc atgcagatcg ttttcctgca ttagccaggc accgaaaatc accgcgacca
     6661 gaccactttc ctcatcagaa agatggacgc cgtattcact ttcaaatccg gccagcgccg
     6721 cgcgggtggt gcgcaccagg cgtgggtaca gtctggcgaa ctcttctggc agggtattat
     6781 caataccgat ggcaaacaaa ctgcgattta acgcctgggc gaggtgggta taaagctgat
     6841 cgcataaccc ctgttcatca taaaaacgaa catttcccag ctcacgaaaa tggtttacca
     6901 gacgtttgat agactgacgc aattgtctgt ctcgctgatg cgcatcacgc aatggatcgg
     6961 gaacccgcaa catggaaaaa agtaatgcca taaacagtgg ctcatcaggc ggtacaggct
     7021 ggagcgcccg acgctgccag tggcgtccaa tttcctgcgc tacctgaaat tcaaggcagg
     7081 attccgccca gcgacgttgg agcggattaa actgaggcgt aattccagcg tggtgttgca
     7141 gcaaacaata ttgcagatac agacacagga aatgaatatc gcgcgtttca aagcgttttt
     7201 gcagccgtcg ggagcagaga ttcactaacg cttgtagatt ggtatcgtcg taaaagttac
     7261 gcgcaattcc gcgccgcttc agttcactct tcagggcggg ggtgaattgg ctggtaataa
     7321 atgacggaca cagacggaaa ccacgtcgta gccagtgaaa taaacacaaa cgttggttaa
     7381 gcactgtacc ttcaacccgg tagctaccgt cataaccagt cgtcagtgtg agttgatgat
     7441 agcgcaggat ctcccgtcct gtttcgctga tatcaagact ggcaatatca tcatccacac
     7501 cattaagctc gctgaaggtt gccatggtgg cggtcaaccc cggctgaaag agcgtcagca
     7561 agatctggca gcggcgctgg ggagccgaaa ggacagatgg tggagcaagc gtaggcatca
     7621 tctgtagatc tcccataagc ataattgata agaatagcta aagtttgctc aggtaaatgg
     7681 cgaagggaat gctaatcatc agaaatgttg gcaagcgtca cagaattttt ctcatcttaa
     7741 actgttctga ccagtggatt agcatgtctt aaggtggttt tgtgaatgat tatcgtgcta
     7801 gcgatcatgg gttagttctg cggattcaac gcgctaagga taaagtccac catctgttcg
     7861 cgataaacat cgccgtgacg atcggaaaaa gcatcaatgt gttcgccatc cgggattagg
     7921 atcaaccgtt ttgtctcttt tgccaggcta tacaactttt cgctgtgctg ccatgggata
     7981 acgtgatcag ctttaccgtg aatgagtaaa agcgggatcg ggctgacgct ggcgatataa
     8041 ttttcgccgc tgtaactctc atcaagtaag tagccactgc cggggatcat ctgattggcg
     8101 atggttgcat aagaggcaaa tgtggagtcg aggatcaccg cacgtatgcc ttcacgatca
     8161 tcctgaccaa taacagcaag aatattcgcc ccgccaatac tctgcccgaa cagcaccagc
     8221 cgttgtgggt ttacatcact gcgatggcgc accacattga tggcactttg cgtatcgtcc
     8281 agcaatccgg cctgggacgg cgtgcctttt gatttaccaa acccgcgata atcaaacata
     8341 aaaacgttga aattacgctc gggtaaccaa ctgaccagcg gccagtgggc ggacatattt
     8401 ccggcattgc cgtgagcatg aatgatggtt gcgatggcat tgtcagcagg gcccgtcgaa
     8461 gaaggaataa accagccttg cagacgagta ccgtccttag ccgtaaattc gacggattcc
     8521 gccgaccagg ggtcgggacc gtaaatttta tcgtcaggat aatagaagaa gttgatggca
     8581 acgcgcggca ctaaataaac gcagaaggca acaacaaaga gaataaacag aattttgaga
     8641 acgcgtttgt tcactggcag cgccataacc tttccttgtg acttctttat catccattaa
     8701 tcggggaaag atggtgggag agtgacgaaa gaaagtaaag cggtgaggaa gtatttcgga
     8761 atagcgcctg aaaggcgagg gcgggtgagt gacatcaccc gcctgagtca ttaacgcttc
     8821 agagcgtcgc ttaactcatc acgcatgttc gccagcatgg ctttcacaac acgcgggtta
     8881 ccagcaacga tgttaccggt cagcatatag ttatgaccac cggtgaagtc gctgacgatg
     8941 ccgcccgctt cacgaaccag cagctcgcct gccgcgaaat cccacggacg cagaccgatt
     9001 tcaaagaaac cgtcaacacg acccgcagcg acgtaagcca gatccagcgc cgcagaaccg
     9061 gtacgacgga agtctgcaca ttcgttgaac agtttgccga cgatgttgat gtaggtagtg
     9121 gcgtactgtt ttgctttgaa cgggaagccg gtcgccagaa tggtaccgtc gagatcgcga
     9181 gcggtgctac cgcgcagacg gtagccgttc agctgtgcgc cctgaccgcg agtggcggtg
     9241 aacagttcgt tacgcatagg atcgtatacc acagcaactt cggtgcggcc tttgatacga
     9301 acggcgatag ataccgcgaa gtgcggcaga cgtttgataa agttggtagt accatccagt
     9361 ggatcgataa cccattgaac atcctgatca gtaccttcaa gttcaccgct ttcttcggtg
     9421 atgatggtgt gctgtgggta agatttacga atcgtgtcga taatcaccgc ttcgacagct
     9481 ttatctacgt tggtcacgaa atcgttactg cctttctggc tcgcttctac agcgtccggg
     9541 gtttcatagt ttttggcaat taaattaccc gccttgcgcg ctgcgcgcac ggcgatgttc
     9601 agcatcggat gcatcggtct ctctcactgg atgttaaaga acgggaaaac ggcgcagagt
     9661 atagcagcga gatcggcatc tgtcttcgtt ttatgataag atgcgtgaat aatctttaga
     9721 tgaagaaaga caatgctgca aaatattcga attgtgctgg tggagacgtc acacaccggc
     9781 aatatgggtt ctgttgcccg tgccatgaaa acaatgggat taaccaatct gtggctggtt
     9841 aatccactgg tgaaacccga ctcccaggcg attgccctgg cagcaggggc cagcgatgtg
     9901 attggtaatg ctcacatcgt cgatacgctc gacgaagcgt tagccggttg tagcttggtg
     9961 gtgggcacca gcgcacgttc ccgcacgctg ccatggccga tgctcgaccc gcgcgaatgc
    10021 ggcctgaaaa gcgtcgctga agcggcaaat accccggtgg cgctggtgtt tggtcgcgag
    10081 cgcgtcggct tgaccaatga agagttgcag aaatgccatt atcatgtcgc gattgcggct
    10141 aacccggaat acagctcgct gaacctggcg atggcggttc aggttattgc ctatgaagtg
    10201 cgcatggcct ggctggcaac tcaggaaaac ggcgagcagg tcgagcatga agagacgccg
    10261 tatccgctgg tcgatgatct ggagcgtttt tatggtcatc tggaacaaac gctgctggca
    10321 accggtttta tccgtgaaaa ccatccgggg caggtgatga ataaattgcg ccgtctgttt
    10381 acccgtgcgc gcccggaaag ccaggaattg aatatcctgc gcgggattct ggcttctatt
    10441 gagcagcaga ataaaggtaa caaggccgaa taacagtagt tgcctgatgc gacgcttaac
    10501 gcgtcttatc aggcctacaa tgtacagaac cgtaggtcgg ataaggtgtt cacaccgcat
    10561 ccgacaacag gtacaaacgc cacgataaaa aaatggcact gaaggttaaa tacccgacta
    10621 aatcagtcaa gtaaatagtt gaccaattta ctcgggaatg tcagacttga ccctgctatt
    10681 caataccccc acttttacaa taaaaaaccc cgggcagggg cgagtttgag gtgaagtaag
    10741 acatgagact gacatctaaa gggcgctatg ccgtgaccgc aatgcttgac gttgcgctca
    10801 actctgaagc gggcccggta ccgttggctg atatttccga acgtcagggc atttcccttt
    10861 cttatctgga acaactgttt tcccgtctgc gtaaaaatgg tctggtttcc agcgtacgtg
    10921 gaccaggcgg tggttatctg ttaggcaaag atgccagcag catcgccgtt ggcgaagtga
    10981 ttagcgccgt tgacgaatct gtagatgcca cccgttgtca gggtaaaggc ggctgccagg
    11041 gcggcgataa atgcctgacc cacgcgctgt ggcgtgattt gagcgaccgt ctcaccggtt
    11101 ttctcaacaa cattacttta ggcgaactgg ttaataacca ggaagtgctg gatgtgtctg
    11161 gtcgtcagca tactcacgac gcgccacgca cccgcacaca agacgcgatc gacgttaagt
    11221 tacgcgctta ataaaaagaa ttcagaatca ggccggagtg ctaaatactc cgtaaacacg
    11281 gtcgtacatc cagccggtag cctgattcct tgcattgagt gatgtacgga gtttatagag
    11341 caatgaaatt accgatttat ctcgactact ccgcaaccac gccggtggac ccgcgtgttg
    11401 ccgagaaaat gatgcagttc atgacgatgg acggaacctt tggtaacccg gcctctcgtt
    11461 ctcaccgttt cggctggcag gctgaagaag cggtagatat cgcccgtaat cagattgccg
    11521 atctggtcgg cgctgatccg cgtgaaatcg tctttacctc tggtgcaacc gaatctgaca
    11581 acctggcgat caaaggtgca gccaactttt atcagaaaaa aggcaagcac atcatcacta
    11641 gcaaaaccga acacaaagcg gtactggata cctgccgtca gctggagcgc gaaggttttg
    11701 aagtcaccta cctggcaccg cagcgtaacg gcattatcga cctgaaagaa cttgaagcag
    11761 cgatgcgtga cgacaccatc cttgtttcca tcatgcacgt aaataacgaa atcggcgtgg
    11821 tgcaggatat cgcggctatc ggcgaaatgt gccgtgctcg tggcattatc tatcacgttg
    11881 atgcaaccca gagcgtgggc aaactgccta tcgacctgag ccagttgaaa gttgacctga
    11941 tgtctttctc cggtcacaaa atctatggcc cgaaaggtat cggtgcgctg tatgtacgtc
    12001 gtaaaccgcg cgtacgcatc gaagcgcaaa tgcacggcgg cggtcacgag cgcggtatgc
    12061 gttccggcac tctgcctgtt caccagatcg tcggaatggg cgaggcctat cgcatcgcaa
    12121 aagaagagat ggcgaccgag atggaacgtc tgcgcggcct gcgtaaccgt ctgtggaacg
    12181 gcatcaaaga tatcgaagaa gtttacctga acggtgacct ggaacacggt gcgccgaaca
    12241 ttctcaacgt cagcttcaac tacgttgaag gtgagtcgct gattatggcg ctgaaagacc
    12301 tcgcagtttc ttcaggttcc gcctgtacgt cagcaagcct cgaaccgtcc tacgtgctgc
    12361 gcgcgctggg gctgaacgac gagctggcac atagctctat ccgtttctct ttaggtcgtt
    12421 ttactactga agaagagatc gactacacca tcgagttagt tcgtaaatcc atcggtcgtc
    12481 tgcgtgacct ttctccgctg tgggaaatgt acaagcaggg cgtggatctg aacagcatcg
    12541 aatgggctca tcattaatcg gtatcggaat caggagaatt tataatggct tacagcgaaa
    12601 aagttatcga ccattacgag aatccgcgta acgtgggttc ctttgacaac aacgacgaga
    12661 acgtcggcag cggcatggtg ggtgcaccgg cctgtggcga cgtgatgaag ttgcagatta
    12721 aagtcaacga tgaaggtatc attgaagacg cgcgttttaa aacttacggc tgcggttctg
    12781 ctatcgcttc cagctccctg gtcaccgaat gggtgaaagg gaagtctctc gtcgaagcgc
    12841 aggcgatcaa aaacaccgat attgctgaag aacttgaact gccgccggtg aaaattcact
    12901 gttctattct ggcagaagac gcgatcaaag ccgccattgc ggactataaa agcaaacgtg
    12961 aagcaaaata agagttgagg tttggttatg tcgattacac tgagcgacag tgcagcagcg
    13021 cgagtaaata cctttctggc taaccgcggc aaagggtttg gcctgcgtct gggcgtgaga
    13081 acctccggat gttcaggtat ggcttatgta ctggaatttg ttgacgaacc gacgccggaa
    13141 gacatcgtgt ttgaagacaa aggcgtgaaa gtcgtggtcg atggcaaaag cctgcaattt
    13201 ctggacggta cgcagctgga cttcgtaaaa gaaggcctga atgaagggtt taaattcacc
    13261 aacccgaacg ttaaagatga gtgtggttgc ggcgaaagct tccacgtttg atgcgcatac
    13321 cgataacccc accgtggtcg cctgcgcgtg gggtttgttt tacctgattc gccgttatcg
    13381 cggcggatcg cagccctgag aatgttatgg attacttcac cctctttggc ttgcctgccc
    13441 gttatcaact cgatacccag gcactgagcc tgcgttttca ggatctacaa cgtcagtatc
    13501 atcctgataa attcgccagc ggaagccagg cggaacaact cgccgccgta cagcaatctg
    13561 caaccattaa ccaggcctgg caaacgctgc gtcatccgtt aatgcgcgcg gaatatttgc
    13621 tttctttgca cggttttgat ctcgccagcg agcagcatac tgtgcgcgac accgcgttcc
    13681 tgatggaaca gctggagctg cgcgaagaac tggacgagat cgaacaggcg aaagatgaag
    13741 cgcggctgga aagctttatc aaacgtgtga aaaagatgtt tgatacccgc catcagttga
    13801 tggttgaaca gttagacaac gagacgtggg acgcggcggc tgataccgtg cgtaagctgc
    13861 gttttctcga taaactgcga agcagtgccg aacaactcga agaaaaactg ctcgattttt
    13921 aatttctgga agctaaacat ggccttatta caaattagtg aacctggttt gagtgctgcg
    13981 ccgcatcagc gtcgtctggc ggccggtatt gacctgggca caaccaactc gctggtagcg
    14041 acagtgcgca gcggtcaggc cgaaacgtta gccgaccatg aaggccgtca cctgctgcca
    14101 tctgttgttc actatcaaca gcaagggcat tcggtgggtt atgacgcgcg tactaacgca
    14161 gcgctcgata ccgccaacac cattagttct gttaaacgcc tgatgggacg ctcgctggct
    14221 gatatccagc aacgctatcc gcatttgcct tatcaattcc aggccagcga aaacggcctg
    14281 ccaatgattg aaacggcggc ggggctgctg aacccggtgc gcgtttctgc ggacatcctc
    14341 aaagcactgg cggcgcgggc aactgaagcc ctggcaggcg agctggatgg tgtcgttatc
    14401 accgttccgg cgtactttga cgatgcccag cgtcagggca ccaaagacgc ggcgcgtctg
    14461 gcggggttgc atgttctgcg cttacttaac gaaccgaccg ctgcggctat cgcctacggg
    14521 ctggattccg gtcaggaagg cgtgatcgcc gtttatgacc tcggtggcgg gacgtttgat
    14581 atttccattc tgcgcttaag tcgcggcgtg tttgaagtgc tggcaaccgg cggtgattcc
    14641 gcgctcggcg gcgatgattt cgaccatctg ctggcggatt acattcgcga gcaggcggac
    14701 attcctgatc gtagcgataa ccgcgttcag cgtgaactgc tggatgccgc cattgcagcc
    14761 aaaatcgcgc tgagcgatgc ggactccgtg accgttaacg ttgcgggctg gcagggcgaa
    14821 atcagccgtg aacaattcaa tgaactgatc gcgccactgg taaaacgaac cttactggct
    14881 tgtcgtcgtg cgctgaaaga cgcgggcgta gaagctgatg aagtgctgga agtggtgatg
    14941 gttggcggtt ctactcgcgt gccgctggtg cgtgaacggg taggcgaatt tttcggtcgt
    15001 ccaccgctga cttccatcga cccggataaa gtcgtcgcta ttggcgcggc gattcaggcg
    15061 gatattctgg tgggtaacaa gccggacagc gaaatgctgc tgcttgatgt gatcccactg
    15121 tcgctgggcc tcgaaacgat gggcggcctg gtggagaaag tgattccgcg taataccact
    15181 attccggtgg cccgcgctca ggatttcacc acctttaaag atggtcagac ggcgatgtct
    15241 atccatgtaa tgcagggtga gcgcgaactg gtgcaggact gccgctcact ggcgcgtttt
    15301 gcgctgcgtg gtattccggc gctaccggct ggcggtgcgc atattcgcgt gacgttccag
    15361 gtcgatgccg acggtctttt gagcgtgacg gcgatggaga aatccaccgg cgttgaggcg
    15421 tctattcagg tcaaaccttc ttacggtctg accgatagcg aaatcgcttc gatgatcaaa
    15481 gattcaatga gctatgccga gcaggacgta aaagcccgta tgctggcaga acaaaaagta
    15541 gaagcggcgc gtgtgctgga aagtctgcac ggcgcgctgg ctgctgatgc cgcgctgtta
    15601 agcgccgcag agcgtcaggt cattgacaat gctgccgctc acctgagtga agtggctcag
    15661 ggcgatgatg ttgacgccat cgaacaagcg attaaaaacg tagacaaaca aacccaggat
    15721 ttcgccgctc gccgcatgga ccagtcggtt cgtcgtgcgc tgaaaggcca ttccgtggac
    15781 gaggtttaat atgccaaaga ttgttatttt gcctcatcag gacctctgcc ctgatggcgc
    15841 tgttctggaa gctaatagcg gtgaaaccat tctcgacgca gcgctgcgta acggtatcga
    15901 gattgaacac gcctgtgaaa aatcctgtgc ttgcaccacc tgccactgca tcgttcgtga
    15961 aggttttgac tcgctgccag aaagctcaga gcaggaagac gacatgctgg acaaagcctg
    16021 gggactggag cccgaaagcc gtttaagctg ccaggcacgc gttaccgacg aagatttagt
    16081 agtcgaaatc ccgcgttaca ctatcaacca tgcgcgtgag cattaacaga ggttagtatg
    16141 ggacttaagt ggaccgatag ccgcgaaatt ggcgaagcac tgtacgatgc gtatcctgat
    16201 cttgatccga aaacggttcg attcaccgat atgcatcagt ggatttgcga tctggaagat
    16261 ttcgacgacg acccgcaggc atccaacgag aaaatcctcg aagcgatttt gttagtctgg
    16321 ctggacgagg ccgaataatt tccgcgtcat gcttcacgcc gcagatgcgt tggctgcgct
    16381 cgttcacccc agtcacatag ttatctatgc tcctggggct tcactcactt gccgccttcc
    16441 tgcaacgcga atcatttagc ggaaaaatcc tggggctgcc aactggcggc ccttttacaa
    16501 agaaggataa ctaaaatgac agaagcgatg aagattaccc tctctaccca acctgccgac
    16561 gcgcgctggg gagaaaaagc aacttacagc attaataatg acggcattac cctgcatttg
    16621 aacggggcag acgatctggg gctgatccag cgtgcggcgc gcaagattga cggtctgggc
    16681 atcaagcatg ttcagttaag cggtgaaggc tgggatgcgg atcgctgctg ggcattctgg
    16741 caaggttaca aagccccgaa aggcacgcgt aaagtggagt ggccggatct ggacgatgcc
    16801 cagcgccagg aactggataa ccgcctgatg atcatcgact gggtgcgtga caccatcaac
    16861 gcaccggcag aagaattggg accatcgcaa ctggcacagc gtgctgttga tctgatcagc
    16921 aacgtcgcgg gcgatcgtgt gacttatcgg atcaccaaag gcgaagatct gcgtgagcaa
    16981 ggttatatgg ggctgcacac cgtcggacgc gcttcagaac gttctccggt attgctggcg
    17041 ctggattaca acccaactgg cgataaagaa gcgccagtgt acgcgtgcct ggtaggtaaa
    17101 ggtatcactt ttgactccgg cggctacagc atcaaacaga ctgcgtttat ggactcgatg
    17161 aagtcggaca tgggcggcgc ggcaacggtt accggggcgc tggcatttgc cattacgcgc
    17221 ggactgaaca agcgcgtgaa gctgttcctc tgctgtgcgg ataacctgat tagcggcaat
    17281 gcgttcaagc tgggcgatat catcacctat cgcaacggta aaaaagttga agtgatgaac
    17341 actgatgccg aagggcgtct ggtgcttgcc gatggtctga ttgatgccag tgcgcagaaa
    17401 ccggaaatga tcattgatgc ggcgaccctc accggggcgg cgaaaactgc gctgggtaat
    17461 gattatcacg cgctgttcag ttttgacgat gcgcttgccg gtcgtttgct ggcgagtgcc
    17521 tcacaagaga acgaaccatt ctggcgtctg ccgctggcgg aattccaccg cagccagctg
    17581 ccgtctaact ttgccgaact gaacaatacc ggaagcgcgg cgtatccggc aggcgcgagc
    17641 acggcggcgg gcttcctgtc gcactttgtt gagaactatc agcaaggctg gctgcatatc
    17701 gactgctcgg cgacttaccg taaagcgccg gttgaacagt ggtctgcggg cgctacggga
    17761 cttggtgtgc gcacgatagc taatctgtta acggcgtaaa ctaatatgcc ggatgcggcg
    17821 taaacgcctt atccggccta caaattctgc aaattcaaca aattgcgaat cccttgtagg
    17881 cctgataagc gtagcgcatc aggcatttgc gccccgaacg ttgaatgaat tgtggttctt
    17941 atgtccgaaa caaaaaacga acttgaagac ctgctggaaa aagcagcaac cgaaccggca
    18001 caccgcccgg cctttttccg tactctactg gaatccaccg tctgggtgcc tggtacggcg
    18061 gcgcagggcg aggctgtggt tgaagatagt gcgcttgatt tacagcactg ggaaaaagaa
    18121 gacggcacca gcgtcattcc ttttttcacc tcgttagaag cgcttcaaca ggcggttgaa
    18181 gacgaacagg catttgtcgt aatgcccgtt cgcacattgt ttgagatgac ccttggcgaa
    18241 acgctcttcc ttaatgccaa actgccgacc ggtaaagaat ttatgccgcg tgaaatcagt
    18301 ttgttgattg gtgaagaggg aaatccgctg agcagccagg aaatcctgga aggcggtgaa
    18361 tcgctgatat tatcggaagt tgcagagccg ccagcacaaa tgattgattc actcaccacc
    18421 ttatttaaaa ccattaagcc ggtgaagcgt gcttttattt gttcaattaa agagaacgaa
    18481 gaggcacagc ctaatttatt tattggcatt gaagccgatg gtgatatcga agaaattatt
    18541 caggcgacgg gaagtgtagc gaccgataca ttacctggcg atgaaccaat cgatatttgt
    18601 caggtgaaaa aaggggaaaa aggaattagc cactttatta ccgaacatat tgtgccattc
    18661 tatgaacgtc gctggggagg ttttttgcgt gactttaaac aaaaccggat aatctaaaat
    18721 atgccgccct aaagggcggc attcgggctt acttgctgct ctgaaggttg ttaaccacca
    18781 gtaaattgtc gccggagaga ctccggttat tatttacttg ggaaaacagc gcaccagaaa
    18841 caataagtac gatacagaag caccgtatct tcattgtgcg gtccatgcga agaccgcatc
    18901 acgacagcac cactggcgat attgccgtta gagcaagcgc tgagacgcgc ttgctctaac
    18961 ggtcgcggaa catcctttcc ttaagccagc gcccgcttgc gagacggcgg aaagggccgc
    19021 agtggataat atactaacag aacaataata actctctttt gcttgacaaa aagagagtta
    19081 ctggcgagta ttgtctccgc tgagacgcgc ttgcattact acggtaatac aacctgctgg
    19141 aaaggccgcg aaccagacca gcaataaaaa accgccaaat tcggcggttt tttattgcct
    19201 gtaaaatact tatttcactg gctcaaccgg taaatctgcc cgcgcgcccc attcactcca
    19261 tgcgccgtcg tacagtttca cgtttggcac atccagcgtc gcgagtgcta acaaaaccac
    19321 ggctgccgtt acaccagagc cgcagctgac gataattggt ttgtcgtagc tgacgccgcg
    19381 accaaaaaat atcgcatcca gttcatcggt cgtttttagt tcgccttcgc gcaccagttc
    19441 cgtccacggc acattcagtg ctccgggaat atgtccgcga cgtaaacctg ggcgaggttc
    19501 atcaacttct gcgttaaaac gtgcagccgg gcgggcatca ataatttgcg ccgtattttc
    19561 atggcttgcc aataatacat cggttacttt caccacggct tcaggattaa acgcggcgtt
    19621 aaactctcct tccggcagct ctactgcacc ttcttctaac agcagatcat cgcgctgcca
    19681 gcctgcaagt ccacccccca gaatcgacac tttctctaca ccaaaggtgc gcagcatcca
    19741 ccatgctcgt ggggctgaga aaagattacc ttcgtcatag acaatcaggt gcttatcctg
    19801 gttaacgcct aattcacgca tcgccacggc gaacgttttc gggcgcggca gcatgtgcgg
    19861 aagcggggaa gtgtgatcag aaagcgcttc gatatcaaaa aacactgcgc cgggaatatg
    19921 tccattcaga tactcctgag caacgttacg atcttcctgt ccaggcgacg ccatgcgggc
    19981 atcgataatc tgaatttccg ggtcatcaat atgttcggcg agccagtcgg ctcctacaaa
    20041 ccatgtcgtg gacataggca tctccatttt tgccggggtt aggctaaatt gtcggatgtt
    20101 tagtgaaaag cgacaagcga aagaggctga tttggctgtc tgagccacaa ttctcactaa
    20161 aattcggatc gta
//
LOCUS       DAJVOY010000065        20043 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVOY010000065 DAJVOY010000000
VERSION     DAJVOY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.065"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..447
                     /locus_tag="OQL90_002041"
                     /pseudo
     CDS             1..447
                     /locus_tag="OQL90_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            469..1431
                     /locus_tag="OQL90_002042"
     CDS             469..1431
                     /locus_tag="OQL90_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1368820.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            1428..2475
                     /locus_tag="OQL90_002043"
                     /pseudo
     CDS             1428..2475
                     /locus_tag="OQL90_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(2567..3916)
                     /gene="accC"
                     /locus_tag="OQL90_002044"
     CDS             complement(2567..3916)
                     /gene="accC"
                     /locus_tag="OQL90_002044"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1368821.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(3927..4397)
                     /gene="accB"
                     /locus_tag="OQL90_002045"
                     /gene_synonym="fabE"
     CDS             complement(3927..4397)
                     /gene="accB"
                     /locus_tag="OQL90_002045"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1368822.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5375..6349)
                     /locus_tag="OQL90_002046"
     CDS             complement(5375..6349)
                     /locus_tag="OQL90_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1368823.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6501..8441
                     /gene="csrD"
                     /locus_tag="OQL90_002047"
     CDS             6501..8441
                     /gene="csrD"
                     /locus_tag="OQL90_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1368824.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8746..9789
                     /gene="mreB"
                     /locus_tag="OQL90_002048"
     CDS             8746..9789
                     /gene="mreB"
                     /locus_tag="OQL90_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1368825.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            9855..10940
                     /gene="mreC"
                     /locus_tag="OQL90_002049"
     CDS             9855..10940
                     /gene="mreC"
                     /locus_tag="OQL90_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1368826.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            10940..11428
                     /gene="mreD"
                     /locus_tag="OQL90_002050"
     CDS             10940..11428
                     /gene="mreD"
                     /locus_tag="OQL90_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1368827.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11437..12030
                     /gene="yhdE"
                     /locus_tag="OQL90_002051"
     CDS             11437..12030
                     /gene="yhdE"
                     /locus_tag="OQL90_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1368828.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12020..13489
                     /gene="rng"
                     /locus_tag="OQL90_002052"
     CDS             12020..13489
                     /gene="rng"
                     /locus_tag="OQL90_002052"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1368829.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13557..17357
                     /gene="yhdP"
                     /locus_tag="OQL90_002053"
     CDS             13557..17357
                     /gene="yhdP"
                     /locus_tag="OQL90_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1368830.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17513..18958
                     /gene="tldD"
                     /locus_tag="OQL90_002054"
     CDS             17513..18958
                     /gene="tldD"
                     /locus_tag="OQL90_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1368831.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19086..20015)
                     /gene="aaeR"
                     /locus_tag="OQL90_002055"
     CDS             complement(19086..20015)
                     /gene="aaeR"
                     /locus_tag="OQL90_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCS1368832.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
BASE COUNT         4756 a         4904 c         5497 g         4886 t
ORIGIN      
        1 atattaatca tcttattgct gattaaccac ttcattctta ctaaaacaac ctttggacga
       61 aaaatttata tcactggtgg taataaagaa gcggcaattt attcagggat aaatgtcacc
      121 cgcttgaaaa ttaaagtctt tatgattaca gccgtgctgg caggtatcag cgggatgatc
      181 ctcgcctcac gcctctattc cgggtaaccg aattccgcgt tgagctacga actggatgct
      241 attgctgcgg ctgtactggg aggcaccagc ctgaacggcg gctatggaac cgtggtgggg
      301 acggtgatcg gcgcattaac tatcggcgtt attaataatg gcatgaactt aatgaatgtt
      361 ccctattttt accagatggt ggttaaagga ttagtcattc ttgtagccgt ttattttgac
      421 gtaagaaata aaagaaaacg cagctaatca tttcaaggaa ttaatcatat gagtaaggtc
      481 ttcacaattg gagaaattct ggttgaaatt atggcgagta aaatcggcca gccatttgat
      541 caaccaggta tctggaacgg cccttatccc agtggtgcgc ccgctatttt tatcgaccag
      601 gtgacacgtc ttggcgttcc gtgcggcatc attagctgtg tggggaatga tggctttggt
      661 gatattaata tacatcgtct ggctgctgac ggtgtggata tcagggggat ttcagtctta
      721 cctctggaag ctaccgggag tgcctttgtt acttatcata actctggcga tcgcgatttt
      781 atttttaata ttaagaatgc cgcttgcggt aaattatcgg cgcagcatgt tgatgaaaat
      841 attctgaagg actgtaccca ttttcatatt atgggctctt ccctgttttc gtttcatatg
      901 gtagacgcag taaaaaaagc ggtaactatc gttaaagcaa atggtggtgt tatttctttc
      961 gaccctaata ttcgtaaaga gatgctcgat attccagaaa tgcgtgacgc cctccacttt
     1021 gttcttgagt taacggatat ctatatgccc agcgagggcg aagtgttgct cttgtcgcca
     1081 cattcgacgc cggagcgtgc aatcgcaggt tttcttgaag aaggtgttaa agaggtgatt
     1141 gtgaagcgcg gcaatcaagg tgccagttac tactccgcga atgagcagtt tcatgttgag
     1201 agctatccgg tggaagaagt tgacccgacc ggtgcgggag actgctttgg tggcgcgtgg
     1261 attgcctgcc gccagctagg gtttgacgtc catcgagcgc tgcaatatgc caatgcctgc
     1321 ggtgcgctgg cggtaaccag acgtggccca atggagggaa cgtctcgcct tgcggaaatc
     1381 gaagcgttca tccagcgcca tgacatgtcc attcgggaag cagcacaatg aaaaacgaaa
     1441 tttggtcgtg gattggcgat ctctcgcagg ttgccggaat taaacactat gagttacgta
     1501 gtggcagagc aaagggtaca gaagcgtttg atgtccgtac tggtgcggga ctggcattta
     1561 cggtggtaaa agatcgcgcg ctggatattg catgggccag ctacaaggac acggcgcttt
     1621 cttttatcac ccccaacggc attgtcgccc ccgctttttt tgagtctcag ggcaacggat
     1681 ttttacgtag cttttacgcg gggttactga ctacctgcgg acttagctac attggcacgc
     1741 cttgtgagga tgaaggagaa acgctggggt tacacgggcg tttggctgcg actccagcag
     1801 aagaggtggg gtatcgcaca gaacggaccg acgacggcat tgaatttgtc atcaatggca
     1861 aagtacggga aacgcgactg ttcggcgaaa atctgacgct ggaaaggacc attcgctgcc
     1921 gttatggcga aaatgtgctg cgcattgaag acaaggtgac taatcatggt ttcacccgcc
     1981 agccgctgca aattctttat cactttaatt acggctggcc tttattatcg ccacaggcca
     2041 gaaatcttgc tgtcggctaa aagcgtaacg ccacgaactc cgcatgcggc ggaagggtta
     2101 accagtcatc tggagatttg taccccacag cccggttttg acgagcaggt ttactatctc
     2161 acgttgaata gcgacagtca ggggatgagt aaagttgctc tggtgaacgc cgaactgggg
     2221 tggggaattt acgagaaatt cgacaccatg cagctaccca actttatcca gtggaaaaat
     2281 ttaggcgcgg gtgagtatgt tatgggactg gaggtcagta atagttttcc tgacggtcgt
     2341 gataaagaac gagcgcaggg gcgtctgccc tttattgagc caggggaaac aaagaaatac
     2401 tgctttgagc tgggaattgt tgatggcgat gctgaaatga gcgcgttgaa agcggaaatt
     2461 gcaggctacc gttagctaac ggcaataaaa acgccgggca gcgttaacag gcccggcgtt
     2521 gattacgttc tttgctaagc gcgatgaaaa caccgcgcgt cacaaattat ttttcctgaa
     2581 gaccgagttt tttctccaga tagtggatgt tagtgccacc atgctggaag ttctcgtcat
     2641 tcatgatgcg gatctgcaga tcaacgttgg ttttgatacc gtcgatgatc agctcctgca
     2701 gcgcattctt catgcgagca atcgccacgt cacggttttc accgtagcaa atcagcttac
     2761 cgatcattga gtcatagtac ggcggtacgg tgtagcccgc gtagatatga gactcccaac
     2821 gcactccaaa accgccaggt gcgtggaaac gggtgatttt gcctgggctt ggcaggaagg
     2881 tgttcggatc ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg tgaacttctt
     2941 cttgcttgat cgacagcggt tgaccggcag cgatacgcag ctgttctttg atcaggtcaa
     3001 cgccggtgat catttctgta accgggtgtt ctacctgaat acgggtgttc atttcgatga
     3061 aatagaactc gccgttttcg aacaggaact cgaaagtacc tgcaccgcga tagccgatat
     3121 caacacacgc tttagcgcaa cgttcgccga tgtagcgacg tagttccggg gtaatgcccg
     3181 gtgctggtgc ttcttcgacc actttctggt ggcggcgctg catggagcag tcacgttccg
     3241 ccagatagat agcgttgccc tgaccgtcag ccagtacctg aatctcgacg tggcgaggat
     3301 tttccaggta tttctccatg taaaccatat cgttgctgaa agcagctttc gcttccgcac
     3361 gggtcatgga gatggattgt gccagttcag cgtcgccgcg cactacgcgc ataccgcgac
     3421 caccaccgcc gccggaggct ttgataatca ccggataacc aatgcgttta gcaatggcac
     3481 ggtttttatc catatcgtcg cccagcgggc cgtcagaacc cggtacgcaa gggacgcccg
     3541 cttttttcat cgccgcgatt gcggatactt tgtcgcccat caggcgaatg gtttctgctt
     3601 tcgggccaat gaagataaag ccggagcgtt caacctgctc ggcaaagttg gcgttctcgg
     3661 agaggaagcc gtaacccgga tggattgcta ctgcgccggt gatttcagcg gcgctgatga
     3721 ttgccgggat gttcagataa ctttttactg acggagcagg gccaatacag accgtttcat
     3781 ctgccagtaa tacgtgtttt agatcgcgat ccgcgctgga gtgcacagcg acagtcttga
     3841 tgcccagttc tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg ttggcaataa
     3901 caattttatc cagcatgttc gcctcgttac tcgatgacga ccagcggctc gtcaaattct
     3961 accggttgtc cactttcgac cagaattgct ttcacggtac cggatttgtc cgcttcgatc
     4021 tggttcatca ttttcatggc ttcaacgatg cacagggtat cgcccacgtt gactttctga
     4081 cccacttcga tgaacgcttt tgcgtccggg cttggggtgc ggtagaaagt accaaccatc
     4141 ggggagcgta cgatgtgacc actgatttcc gctgctgctg gcgcttccat ggaaggaacg
     4201 gtcgccggag cggctgcgtt agattgagct ggctgctgca tcattggtgc agcgtaagct
     4261 tgttgcatca cagggaaact tgcggcagga gctgcacggc taatgcgtac tgactcttcg
     4321 ccttcagaaa tttccagttc ggagatgcct gattcttcaa ccagctcgat cagtttttta
     4381 atcttacgaa tatccatgag tgggttccgt actctttgtt tagtgtgatt gtgacaggcg
     4441 tttcaccgcc gtctgtaaag cgtatgcccc gacagctgta tgcatagcga taaattccag
     4501 caggccggga cgccggtcta ttttgcccca gagtatcaat gttagacttg acggacattg
     4561 tgcagcgatc tgctgctatc ctccggcaaa aaacaaaata taccttcgtt gcgccaatgt
     4621 caccttttcc gcagcgcaaa aactgcgtca gggaggacga ggtcgcacat tataacgatt
     4681 tcgtagcaat tggcagctaa atactggtct tatcagggaa gataatcaac agctaacatg
     4741 taaataacct tcaacaccgt gtaatttgca acaagccgca caattcacga aattagcgcc
     4801 atcatcgacg gaacttctta taacgtaagg tgaaaagctg taaagccagc cctgcgaaga
     4861 tgaggggctg caatgagata atcttcacag accacaaata atgtagggat gccaggatcg
     4921 cgacaagata gacgacatta cgcagcggtt gctggggatt acccaatttt cactgcatcg
     4981 tctggataga tgtgaatgct aaagcacgca aaatcaaccg actgacaatt ctcagcatta
     5041 aataggttcg agtggttaat ttgcagtcaa ataaaatcag attattcacc ccatggtacc
     5101 agaaatcagt aacaggttaa gcgtagtgtc gcccaggcaa agcaccataa acctaacagg
     5161 cgacggaggc gtatcaataa tggctgttta gaggagtatc aatgcgcatc aatttgtggt
     5221 cagtcgaaat catgactttc cagtttggat gatgttgtac aacttcctga ccaaggtgtt
     5281 cgacgaaagc tgtccggcta accgcttttc ccgttaaccg attgaataat gatgtcaaaa
     5341 tgagttccct ctcttattat tccttgctaa atggttagtt aaccttcacc agcgtgcgac
     5401 cctggatctg gttattaatg atggcctcgg caaagttcgg tgcctctgac agagatatct
     5461 cttttgccgc ctgggtatag aatgattccg gtaaatcggc gaccagtcgc tgccaggctt
     5521 gtgcgcggcg ttctggtggc gtcattactg aatccacccc ttgcaaacgg acattacgca
     5581 gaataaatgg catgaccgtg gttggcagag taaaaccacc cgccagacca caggccgcca
     5641 cgcagccgcc gtaattcatt tgcgccagca cttttgccag cactttgtcg ccaacagtgt
     5701 caattgcccc agcccagact tgtttttcca gaggacggga ttcggcaaac tcatcacgag
     5761 ggagaatacg gctggcacct aaacttttca gatattcatg ggtactttcg cgaccggaaa
     5821 cggcaacgac ctgataaccc aacttatgca gcagcgccac ggcggtactg ccgacgccac
     5881 cactggcacc cgtcacgaca atctccccgt cctgcgggcg aacaccggca tcttccagcg
     5941 ccatcacaca cagcatggcg gtaaaaccgg cagtaccgat aattattgct ttacgcgcgt
     6001 ccagcccttg cggcatggca accagccagt cacctttcac tcgcgcctgc tccgccagcc
     6061 caccccagtg gttttcacca acgccccagc cagtgagtaa cacctcctga ccggcatgaa
     6121 aacgcggatc ttcgctggtg cgtacagttc cggcaaaatc gatcccagga atcatcggaa
     6181 aattacggat gatttttccc ttaccggtaa tcgccagcgc atctttatag ttcaggctcg
     6241 accagtgaac atcgaccatg acatcgccct ccggcaggcg actttcgtcc agagtctgta
     6301 ctgatgcgag agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata agtggtcctc
     6361 atgtgcatga tggattggaa aataatttct gaagattata ctcgctaatg gaaaagcaaa
     6421 gccgatgaag cgcaataaat tgcgagataa atctgatttg ctagtatgcc cgcttcctca
     6481 ctatcggagt taacacaagg atgagattaa cgacgaaatt ttcggccttt gttacgctgc
     6541 tcaccgggtt aacaattttt gtgactttgc tgggctgttc gctaagtttc tacaacgcaa
     6601 ttcagtataa gtttagtcat cgcgttcagg cggtggcgac ggcgattgat acccaccttg
     6661 tgtcgaatga cttcagcaca ttaaggccac aaattaccga attaatgatg tcggcagata
     6721 tcgttcgtgt agacctgctc catggtgata aacaggttta taccctggcc agaaatggta
     6781 gttatcgtcc ggttggctcc agcgatctgt ttcgtgaact gagcgttccg ttgataaagt
     6841 atccggggat gtcgctgcgt ctggtttatc aggatccgat gggcaactat ttccattcgt
     6901 tgatgaccac cgcgccgctc acgggggcga ttagctttat cattgttatg ctcttcttgg
     6961 cggtacgctg gttacaacgg caacttgccg ggcaagaatt gctggaaacc cgggctactc
     7021 gtatcttaaa cggtgagcgt ggctctaatg tgttgggaac catctatgaa tggccgccca
     7081 gaaccagcag tgcgctggat acgctgcttc gtgaaattca gaacgcacgc gaacaacaca
     7141 gccgtcttga tacgctgatc cgctcttatg ccgcccagga cgtgaaaacc ggcctcaata
     7201 accgactctt tttcgataat cagttagcaa cgttactgga agatcaggag aaagtaggta
     7261 cccacgggat cgtgatgatg attcgtctgc cggatttcaa tatgttgagc gatacttggg
     7321 ggcacagcca ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg acatttatga
     7381 tgcgctaccc tggcgcactg ctggcgcgtt accaccgcag tgattttgct gcgctgttac
     7441 cgcaccggac gttaaaagag gcagagagca tcgccggtca gttaatcaaa gccgtcgata
     7501 ccttgccgaa caataaaatg ctcgatcgcg acgatatgat ccacattggt atctgcgcct
     7561 ggcgtagtgg tcaggatacc gagcaggtaa tggaacatgc agagtctgcc acgcgtaatg
     7621 cgggattgca gggcggcaat agctgggcta tttacgatga ctcgttgcct gaaaaaggac
     7681 gcggtaatgt tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc ggcccgcgcc
     7741 tttatcaaaa accggcggtt actcgcgaag gtcaggttca tcatcgcgaa ctcatgtgcc
     7801 gcatcttcga tggtaatgaa gaggttagct cggcggagta tatgccgatg gtcttgcagt
     7861 ttggcttatc ggaagagtat gaccgtctgc aaatcagccg tcttattcca ctattgcgtt
     7921 actggccaga ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt cgcccgcgtt
     7981 ttcagcgttg gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga aaacgcataa
     8041 ttattgaact tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa cctgttattc
     8101 gtttagtgaa tgctttaggg gtacgggtag ccgtcaacca ggctggtttg acgctggtaa
     8161 gcaccagttg gatcaaagaa cttaatgttg agttactcaa gctccatccg gggctggtca
     8221 gaaacattga gaagcgaacg gagaaccagc tgctggttca aagcctggtg gaagcctgct
     8281 ccgggaccag cacccaggtt tacgccaccg gtgtgcgttc gcgaagcgag tggcagaccc
     8341 tgattcagcg cggtgttaca ggcgggcaag gggatttttt cgcgtcctca cagccacttg
     8401 atactaacgt gaaaaaatat tcacaaagat actcggttta acctgccgtt taatccgttt
     8461 tcacgtagaa taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc gccagattgt
     8521 tgcagcacat atgcagatga atgaccttac gcggttgcaa acaggcgagg aatgctgctg
     8581 atgcattaag cctttctgga ctcaggcaga gatttgtaac aaaggaaacg aactgcacta
     8641 attttcaccg tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt tggtaaagta
     8701 agcggatttt cttttccgcc ccagctttca ggattatccc ttagtatgtt gaaaaaattt
     8761 cgtggcatgt tttccaatga cttgtccatt gacctgggta ctgcgaatac cctcatttat
     8821 gtaaaaggac aaggcatcgt attgaatgag ccttccgtgg tggccattcg tcaggatcgt
     8881 gccggttcac cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat gctgggccgt
     8941 acgccgggca atattgctgc cattcgccca atgaaagacg gcgttatcgc cgacttcttc
     9001 gtgactgaaa aaatgctcca gcacttcatc aaacaagtgc acagcaacaa ctttatgcgt
     9061 ccaagcccgc gcgttctggt ttgtgtaccg gttggcgcga cccaggttga acgccgcgca
     9121 attcgtgaat ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga agaaccgatg
     9181 gctgccgcaa ttggtgctgg cctgccggtt tctgaagcga ccggttctat ggtggttgat
     9241 atcggtggtg gtaccactga agttgctgtt atctccttga acggcgtggt ttactcctct
     9301 tccgtgcgca ttggtggtga tcgtttcgac gaagctatca tcaactatgt gcgtcgtaat
     9361 tacggttctc tgatcggtga agccaccgca gaacgtatca agcacgaaat cggttcggct
     9421 tacccgggcg atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc agaaggtgtt
     9481 ccacgcggtt ttaccctgaa ctccaacgaa atcctcgaag cactgcagga accgctgacc
     9541 ggtattgtga gcgcggtaat ggttgcactg gaacagtgcc cgccggaact ggcttccgac
     9601 atctccgagc gcggcatggt gctcaccggt ggtggcgcac tgctgcgtaa ccttgaccgt
     9661 ttgttaatgg aagaaaccgg cattccagtc gttgttgctg aagacccgct gacctgtgtg
     9721 gcgcgcggtg gcggcaaagc gctggaaatg atcgacatgc acggcggcga cctgttcagc
     9781 gaagagtaat cggatgcagg caggggaaat gtctgtttac cctgcctagt ctgatacgag
     9841 aatacgcata acttatgaag ccaattttta gccgtggccc gtcgctacag attcgcctta
     9901 ttctggcggt gctggtggcg ctcggcatta ttattgccga cagccgcctg gggacgttca
     9961 gtcaaatccg tacttatatg gataccgccg tcagtccttt ctactttgtt tccaatgctc
    10021 ctcgtgaatt gctggatggc gtatcgcaga cgctggcctc gcgtgaccaa ctagaacttg
    10081 aaaaccgggc gttacgtcag gaactgttgc tgaaaaacag tgaactgctg atgcttggac
    10141 aatacaaaca ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg cgtcaggatg
    10201 agcagaaaat ggtgactcag gttatctcca cggttaacga tccttatagc gatcaagttg
    10261 ttatcgataa aggtagcgtt aatggcgttt atgaaggcca gccggtcatc agcgacaaag
    10321 gtgttgttgg tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg ctgatttgtg
    10381 atgcgaccca cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta attgcagccg
    10441 gtaacggttg tacagatgat ttgcagcttg agcatctgcc agcgaatacg gatattcgtg
    10501 ttggtgatgt gctggtgacc tccggtctgg gcggtcgttt cccggaaggc tatccggtcg
    10561 cggttgtctc ttccgtaaaa ctcgataccc agcgcgctta tactgtgatt caggcgcgtc
    10621 cgactgcagg actgcaacgt ttgcgttatc tgctgctgct gtggggcgcg gatcgtaacg
    10681 gcgctaaccc gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt ctgatgcaga
    10741 tgatgccgca ggtattgcct tcgccagacg cgatggggcc aaagttacct gaaccgacaa
    10801 cggggatcgc tcagccgact ccgcagcaac cgacgacagg aaatgcagct actgcgcctg
    10861 ctgctaatcg ctctccacaa agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc
    10921 gtgcgccggg agggcaatag tggcgagcta tcgtagccag ggacgttggg taatctggct
    10981 ctctttcctc attgcgctgt tgctgcaaat catgccctgg ccggataacc tgattgtttt
    11041 ccggccaaac tgggtgttac ttatcttgtt gtattggatc ctggccttgc cccatcgcgt
    11101 aaatgtgggc acaggttttg tgatgggtgc catactggat ctgatcagcg gctcgacgct
    11161 tggcgtacgc gtattggcga tgagcatcat tgcttacctg gtggcgctga aataccagct
    11221 tttccgcaac ctcgcattat ggcagcaggc gctagtcgtc atgttgcttt cgctggtggt
    11281 ggatattatt gttttctggg cagagttttt agtgattaac gtctctttca gaccggaagt
    11341 gttctggagt agtgtagtca atggggtgct ctggccgtgg attttcttgc tgatgcgtaa
    11401 agtccgtcag cagtttgcag tgcaataaag gtttctatga cttctctgta tttagcttcc
    11461 ggttctccgc gtcgtcagga gttacttgcg caacttggcg tgacctttga acgtattgtt
    11521 acgggcattg aggagcagcg tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg
    11581 gcacacaaga aagcacaggc gggtgtcgcg caaacggcgc aggatctccc ggtgctgggt
    11641 gcggatacta tcgttatcct gaatggagaa gtgctggaga aaccgcgcga cgcagagcat
    11701 gcggcgcaga tgttgcgcaa attatcgggt cagacccatc aggtgatgac ggcagtggtg
    11761 ttggccgaca gccagcacat tctcgattgc ctggtggtca ccgatgtgac tttcagaacg
    11821 ttaacagacg aagacatcgc gggctatgtc gccagcggtg aaccgttaga taaagcaggt
    11881 gcatacggta ttcaggggct gggtggctgt tttgtcagga agataaatgg cagctatcac
    11941 gccgtagtcg gcttaccgct ggttgaaacg tatgaattat taagtaattt taacgcactg
    12001 cgtgagaaaa gggataaaca tgacggctga attgttagta aacgtaacgc cttcggaaac
    12061 gcgagtggcg tatattgatg gcggtattct gcaggaaatt catattgaac gtgaggcgcg
    12121 acgcggaata gtaggcaata tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca
    12181 ggcggctttt gtagatattg ggctggataa agccgcgttt cttcatgcat ccgatatcat
    12241 gccgcacacc gaatgtgtgg cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc
    12301 ggaactggtt cgtcaggggc aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac
    12361 taaaggtgcg cgcctgacca ccgatatcac gctgccttct cgctatctgg tgtttatgcc
    12421 aggggcttct cacgttgggg tttcccaacg tattgaaagc gaatcagaac gtgaacgcct
    12481 gaaaaaagtg gtcgcagagt attgcgacga gcagggcggg tttatcatcc gtaccgcagc
    12541 ggaaggggtt ggcgaggctg aactggcctc cgatgccgct tatctgaaac gcgtctggac
    12601 caaagttatg gagcgtaaaa aacgcccgca gacccgttat cagctgtacg gcgaactggc
    12661 gctggcgcag cgtgttctgc gtgatttcgc cgacgccgaa ctggaccgca ttcgcgttga
    12721 ctcacgcctg acttacgaag cattgctgga gtttacctcg gagtacattc ccgagatgac
    12781 cagcaagctg gagcattaca ccggacgcca gccgattttc gatctctttg atgtcgaaaa
    12841 cgaaatccag cgagcgctgg aacgcaaagt agaactgaaa tccggtggtt atctgattat
    12901 cgaccagacc gaagcgatga ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg
    12961 caatctggac gacaccattt tcaataccaa tattgaagcg acgcaggcta tcgctcgcca
    13021 gttacggttg cgtaatctgg gtgggattat cattattgat ttcatcgata tgaataatga
    13081 agatcaccgc cgccgcgtgc tgcactcgct ggagcaggcg ttgagcaaag accgggtgaa
    13141 aaccagcgtt aatggttttt cggcgctggg gctggtggag atgacgcgta aacgcacccg
    13201 cgaaagcatt gagcacgtac tgtgtaacga atgcccaacc tgccacggtc gcggaacggt
    13261 gaaaaccgtg gaaacggtat gctatgaaat catgcgcgag attgttcgtg tccaccatgc
    13321 ttacgactcc gaccgtttcc tggtctatgc ttctccggca gtagctgaag ccttgaaagg
    13381 cgaagagtca cactcgctgg cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca
    13441 aattgaaccg ctctataacc aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg
    13501 gccgccatcc ggcaaagggt ttttgagtca catttttagc agacaaggag tgacgggtga
    13561 ggcgattgcc ggggatttta ctgcttactg gagccgcgct cgttgtgatc gcagccctgc
    13621 tggttagcgg cctgcgtatt gctttaccgc atcttgacgc ctggcgtccg gaaatcctca
    13681 acaaaataga atccgcgact ggcatgccgg tagaagccag tcagctctca gccagctggc
    13741 agaattttgg cccgacgctt gaagcacacg acatccgtgc agaactaaaa gatggcggcg
    13801 aattttcggt taaacgcgtt actctggcgc tggatgtctg gcagagcttg ttacatatgc
    13861 gctggcagtt tcgcgacctc actttctggc agctgcgctt tcgcaccaac actcctatca
    13921 ccagcggtgg tggtaatgat agcctggaag ccagtcacat cagcgatctg tttcttcgtc
    13981 aatttgacca tttcgatctc cgcgacagtg aagtcagttt tctgacgcca tccggtcagc
    14041 gcgccgagct ggcgatccca caactcacct ggctgaacga tccacgtcga caccgtgcgg
    14101 aaggcctggt aagcctctcc agccttaccg gacagcacgg cgtgatgcag gtgcgcatgg
    14161 atttgcgcga tgatgagggg ttgttaagca atggtcgcgt ctggctacag gcggatgaca
    14221 tcgacctgaa gccgtggctc ggtaaatgga tgcaggacaa tattgctctg gaaacggcac
    14281 agttctccct tgaaggctgg atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct
    14341 ggctgaaaca gggcggtgcc agctggttgg gcgagaagca aacgcatacg ctgtcggtgg
    14401 ataatctgac cgcgcatatt acgcgtgaaa atccgggctg gcagttctct attcccgata
    14461 cacggatcac gatggacggc aaaccctggc cgagcggagc attgacgctg gcctggatac
    14521 cggaacagga cgttggcggc aaagacaata aacgcagtga cgaactccgg attcgcgcca
    14581 gtaatctgga gctggcaggc ctggagggca tacgcccgct ggccgcgaaa ctttcacctg
    14641 cactgggtga tgtttggcgc tccacacaac cgagcggcaa gattaacact ctggcgctgg
    14701 atatcccgct tcaggcggca gacaagaccc gttttcaggc atcgtggagc gatctggcct
    14761 ggaagcaatg gaaattatta ccgggtgcgg aacacttctc cgggacgctt tccggcagcg
    14821 ttgaaaatgg tttgcttacc gcgtcgatga agcaggcaaa gatgccttac gaaacggtat
    14881 tccgtgcgcc actggaaatc gccgacggcc aggcaactat aagctggctg aacaatgaca
    14941 aaggtttcca cctggatggg cgtaatattg acgttaaagc caaagctgtc catgcgcgcg
    15001 gcggttttcg ttacctgcaa cctgctaacg atgaaccctg gctgggtatt ctggctggca
    15061 tcagtaccga tgatggttca caagcctggc gctatttccc ggaaaacttg atgggtaaag
    15121 acctagttga ttacttaagt ggcgcgattc agggcggtga agcggataac gcgacgctgg
    15181 tttatggtgg caatccgcaa ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc
    15241 tggtgccgct gcgtaacgcg aagtttgcct tccagccgga ctggcctgca ttaactaacc
    15301 ttgatattga actggacttt attaacgacg gtttatggat gaaaaccgat gacgttaatc
    15361 tgggcggcgt gcgcgcgagt aatctcaccg cagtgatccc tgactactcg aaagaaaaac
    15421 tgctgattga cgctgacatt aaaggtccgg gtaaagccgt tggcccttac tttgatgaga
    15481 caccgctgaa agattctctg ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga
    15541 atgctcgctt acatcttgat atcccgctga acggcgaact ggtaaccgcg aaaggtgaag
    15601 tgacgctgcg taataacagt ctgtttatca aactactcga cagcaccctg aaaaatttga
    15661 gcggtaaatt cagctttatc aatggcgatc tgcaaagtga accactgaca gcaagctggt
    15721 ttaatcagcc gttgaacgtg gatttttcca ccaaagaagg ggcaaaagcc taccaggttg
    15781 cggtaaacct caacggtaac tggcaaccgg cgaaaaccgg cgttctgcct gcagcggtga
    15841 acgaagcatt gagtggcagc gtggcgtggg atggtaaagt gggcattgat ctgccttatc
    15901 atgctggcgc gacctataac gtagagctga acggcgatct gaataatgtg agcagtcact
    15961 taccttcacc gttagccaaa cctgcgggtg aaccactgcc ggtaaacgtt aaggttgatg
    16021 gcaatctcag cagctttgaa ttaaccggac aggctggtgc ggataaccat ttcaatagcc
    16081 gctggttgct tggtcaaaag ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa
    16141 cgctcccgcc gttgccggaa caaagtggcg ttgaactcaa tatgccgccg atgaatggtg
    16201 ccgagtggct ggccctgttc cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt
    16261 tcccacaaca cataacgtta cgtacgccta tgttgtcact gggaaatcag caatggaata
    16321 acctgagtat tgtttcgcaa ccgacggcaa atggcaccct ggttgaggcg caggggcgtg
    16381 aaatcaatgc cacgctggcg atgcgtaata acgcgccgtg gctggcgaat atcaaatatc
    16441 tttattacaa cccgagcgtg gcgaaaactc gtggtgattc aacaccgtca tcacctttcc
    16501 cgacaacgga gcgcattaac ttccgtggct ggccggacgc acaaatacga tgcacagagt
    16561 gctggttctg ggggcaaaaa ttcggtcgta ttgacagtga tctcaccatt tctggcgata
    16621 cgttaacgct gaccaatgga ctgattgata ctggtttctc gcggcttact gccgatggtg
    16681 aatgggttaa taatccgggg aatgaacgta cctcgctgaa aggaaaactg cgcgggcaga
    16741 aaattgatgc cgccgcagaa ttttttggtg tcacgacgcc catacgccag tcgtcattta
    16801 atgtggatta cgatttacac tggcgcaaag caccctggca accagatgag gcgacgttga
    16861 atggcatcat tcatactcaa ctgggtaaag gcgaaattac cgaaatcaat accggacatg
    16921 ccgggcaatt gctgcgctta ttgagcgttg atgccctgat gcgtaagctg cgttttgatt
    16981 tcagagacac ttttggcgaa gggttctatt ttgactccat tcgcagcacc gcgtggatta
    17041 aagacggcgt tatgcacacc gacgacacgc tggtggatgg cctggaggcg gatatcgcca
    17101 tgaaagggtc ggtaaatctg gtacgtcgcg acctgaatat ggaagcggtt gtcgcaccag
    17161 agatttctgc gacggtgggc gtggctgcgg cttttgcggt taaccccatt gttggcgcgg
    17221 cagtatttgc agccagtaaa gtgctggggc cgctgtggag caaagtctcc attttgcgct
    17281 atcacatttc gggtccgctg gacgatccgc aaatcaacga agtgttgcgc caaccgcgta
    17341 aagaaaaagc gcaatgattt gacgagggcg cgtaattgcc ccaatctcat atgataatcg
    17401 ttgccaaagg ccaacgagcc agaacataac cgtaggtcgg atagggcgtt cacgccgcat
    17461 ccggcagccg taaaaaatcc tctactgcag taactaacga gtagcaaaaa cgatgagtct
    17521 taacctagta agtgaacaat tgctagcggc gaacggcctg aaacatcagg acttgttcgc
    17581 gatcctcggt caactggccg aacgtcgcct tgattatggc gatctctatt ttcagtcgag
    17641 ctatcacgaa tcctgggttt tagaagaccg cattattaaa gatggttctt acaacatcga
    17701 tcagggcgtt ggcgtgcgtg caatcagcgg tgaaaaaacc ggatttgctt acgctgacca
    17761 aatcagcctg ctggcgctgg aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag
    17821 tggtgatggc aaagtacaga cgctgggcgc ggtagggcat agcccgttgt atacctcggt
    17881 agatccgctg caaagcatga gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa
    17941 ggttgctcgc gaagcggaca agcgcgtaca ggaagtgact gccagcctca gtggtgtcta
    18001 tgaattaatt ctggttgcgg ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt
    18061 gcgtctttcc gtgagcgttc tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg
    18121 cggtggcggt cgttttggtt atgagttctt ccttgccgat cttgacggcg aagtccgtgc
    18181 ggatgcatgg gcaaaagaag cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc
    18241 accagcgggc accatgccgg tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga
    18301 agcggtaggt cacggtctgg aaggcgactt caaccgccgc ggcacttcag tatttagtgg
    18361 gcaggtcggg gagctggtgg cttcagaact gtgtaccgtg gttgatgacg gcacgatggt
    18421 cgatcgccgt ggttcggtgg cgattgatga cgaaggtacg ccaggccagt acaacgtgct
    18481 gattgagaac ggcattctga aaggctacat gcaggataaa ctcaacgcgc gtttgatggg
    18541 gatgacgccg actggcaacg gtcgccgtga atcctacgcc catctgccca tgccgcgtat
    18601 gaccaacacc tatatgctgc cgggtaaatc gaccccgcag gaaattattg aatccgttga
    18661 gtacggtatc tatgcaccga actttggtgg cggtcaggtg gatatcacct ccggcaaatt
    18721 cgttttctcc acttcagaag catatctgat tgaaaacggt aaagtaacga agccggtgag
    18781 aggcgcaacg ttgattggtt ccggtatcga aaccatgcag cagatttcga tggttggcaa
    18841 cgacctgaaa ctggataacg gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt
    18901 tggcgtgggc cagccaacgc tgaaagttga taacctgact gttggcggta ctgcgtaata
    18961 atttcattat tttcagaagg ataattaatc tttctacgtg cacgaacggt cccctcgctc
    19021 ctctggggtt agggtgaggg gaacccgttg gcacaggttt gtagaacgta acagtacaat
    19081 atgaattact tctctttccc gcgcccgtgc atctcctgaa acaatttacc gacctcaaca
    19141 aaataatccg tcagcgagtt gatcacgacc tgtaccttca gcggcagctt atctttttcg
    19201 gtatataacg cataaaccgg gcgtggatct gactgataac gcggcagcag gatctccagc
    19261 tccccacgat tgatctcgtt gatcacccac atcagcggca cgtaggcgat cccggcaccc
    19321 gccgttagcc agcgcaccag cgtcatcgga tcattagtca caaatctccc ttgcgggatc
    19381 aggcgagtcg agatcccttc cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg
    19441 tattcaagcc atgaatggct acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg
    19501 agatagcttt tcgcggcgca caccaccatt ggcatcgcgc caagacggcg ggaaaacagg
    19561 ctggaatcct gcaacgcgcc gacgcggatc accacatcca gaccgtcggc aatcaggtcg
    19621 ggggctggaa ttccggtaac cagattgacg ctcaaacctg ggtattcttt cagcattttg
    19681 gctgtcagcc cggcgagaac attttgtgcc atagttgaag aacagccaat gcgtagcgtc
    19741 ccgatggggg tgttattgaa agcatacagt tgctcatgaa catcctgcac ttcatgaagc
    19801 atacgacggc agccctggta gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg
    19861 ctacggttta acagctttac ctgcaactca tcttccagtt ttgacaccgt ctgactgatg
    19921 gacgaaacgc tcatctgtag ctgtctggcg gcggcggtaa aagagccaaa ttcaactact
    19981 ttggcaaaca ccgacatgcg ttttagtcgt tccattattc actctgactt aaaagtgatt
    20041 tag
//
LOCUS       DAJVOY010000066        19875 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVOY010000066 DAJVOY010000000
VERSION     DAJVOY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.066"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            54..830
                     /gene="eutC"
                     /locus_tag="OQL90_002056"
     CDS             54..830
                     /gene="eutC"
                     /locus_tag="OQL90_002056"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HCS1368833.1"
                     /translation="MTSESCALDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTG
                     RAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDM
                     GRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYEELLPPLMAGLKQAGLKVG
                     TPFFVRYGRVKIEDQICEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEAD
                     RTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR"
     gene            840..1499
                     /gene="eutL"
                     /locus_tag="OQL90_002057"
     CDS             840..1499
                     /gene="eutL"
                     /locus_tag="OQL90_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1368834.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1512..2012
                     /gene="eutK"
                     /locus_tag="OQL90_002058"
     CDS             1512..2012
                     /gene="eutK"
                     /locus_tag="OQL90_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1368835.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2058..3110
                     /gene="eutR"
                     /locus_tag="OQL90_002059"
     CDS             2058..3110
                     /gene="eutR"
                     /locus_tag="OQL90_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCS1368836.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OQL90_002060"
     CDS             complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OQL90_002060"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1368837.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCNDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OQL90_002061"
     CDS             complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OQL90_002061"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1368838.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5102..5527
                     /locus_tag="OQL90_002062"
     CDS             5102..5527
                     /locus_tag="OQL90_002062"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1368839.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5514..5963
                     /locus_tag="OQL90_002063"
     CDS             5514..5963
                     /locus_tag="OQL90_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1368840.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6170..6745
                     /locus_tag="OQL90_002064"
     CDS             6170..6745
                     /locus_tag="OQL90_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1368841.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6841..7740
                     /gene="yfeX"
                     /locus_tag="OQL90_002065"
     CDS             6841..7740
                     /gene="yfeX"
                     /locus_tag="OQL90_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1368842.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OQL90_002066"
                     /pseudo
     CDS             complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OQL90_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OQL90_002067"
     CDS             complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OQL90_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCS1368843.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OQL90_002068"
     CDS             complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OQL90_002068"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1368844.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11595..12452
                     /gene="murR"
                     /locus_tag="OQL90_002069"
     CDS             11595..12452
                     /gene="murR"
                     /locus_tag="OQL90_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1368845.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12581..13372
                     /gene="ucpA"
                     /locus_tag="OQL90_002070"
     CDS             12581..13372
                     /gene="ucpA"
                     /locus_tag="OQL90_002070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1368846.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13676..14692
                     /gene="cysP"
                     /locus_tag="OQL90_002071"
     CDS             13676..14692
                     /gene="cysP"
                     /locus_tag="OQL90_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1368847.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14692..15525
                     /gene="cysT"
                     /locus_tag="OQL90_002072"
     CDS             14692..15525
                     /gene="cysT"
                     /locus_tag="OQL90_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1368848.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15525..16400
                     /gene="cysW"
                     /locus_tag="OQL90_002073"
     CDS             15525..16400
                     /gene="cysW"
                     /locus_tag="OQL90_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1368849.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16390..17487
                     /gene="cysA"
                     /locus_tag="OQL90_002074"
     CDS             16390..17487
                     /gene="cysA"
                     /locus_tag="OQL90_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1368850.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17621..18532
                     /gene="cysM"
                     /locus_tag="OQL90_002075"
     CDS             17621..18532
                     /gene="cysM"
                     /locus_tag="OQL90_002075"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1368851.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18535..18903)
                     /locus_tag="OQL90_002076"
     CDS             complement(18535..18903)
                     /locus_tag="OQL90_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1368852.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            19008..19859
                     /gene="pdxK"
                     /locus_tag="OQL90_002077"
                     /gene_synonym="thiJ"
     CDS             19008..19859
                     /gene="pdxK"
                     /locus_tag="OQL90_002077"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1368853.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4571 a         5044 c         5610 g         4650 t
ORIGIN      
        1 cggcccccgc gctgtcagaa gcaaagtgca ccaccaccac ctgtgcggca ccggtgacct
       61 cggaaagctg cgcgctggat ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa
      121 acccgcatcg cgcagacgta ttaacagaac tgcgtcgcag caccgtggcc cgcgtttgta
      181 ccggtcgtgc cggtccgcgt ccgcgtaccc aggcgttgct gcgtttcctg gccgatcact
      241 ctcgttcgaa agataccgta ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct
      301 tgctggaagt acgctccgaa attagcgaca aaaatctcta cctgactcgc ccggatatgg
      361 gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg
      421 acgtacaggt cgttatttct gatggcctgt caactgatgc catcaccgtg aactacgaag
      481 agctcctgcc gccgctgatg gcgggcctga aacaggccgg gctgaaagtc ggcacgccat
      541 tctttgttcg ttatggtcgc gtgaagattg aagatcagat ctgtgagatc ctcggcgcga
      601 aagtggtgat cctgctggtg ggggaacgtc cggggttggg tcagtcagaa agcctctcct
      661 gttacgccgt ttactcgccg cgtatggcga ccaccgttga ggccgatcgc acctgcatct
      721 ctaacattca ccagggcggc acgccgccgg ttgaagccgc agccgtcatt gtggatttgg
      781 cgaaacgtat gctggagcag aaagcatccg gcatcaacat gacccgataa ggaggcatca
      841 tgccagcttt agatttgatt cgaccgtcgg taaccgccat gcgggtgatt gcctccgtta
      901 acgctgaatt tgcgcgtgaa ctaaaattgc cgccgcatat tcgtagcctc gggctgattt
      961 ctgctgattc cgatgacgtc acctacattg cggctgacga agcgaccaag caggcgatgg
     1021 tcgaagtggt atatggccgc tcgctgtatg cgggagcagc acacggaccg tcaccgactg
     1081 ccggtgaagt gctgattatg cttggtgggc caaacccggc ggaagtgcgt gctggtctgg
     1141 atgcgatggt tgcgcatatt gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa
     1201 acaccgcatt cctggcacat gtagtttcgc gtaccggttc ttatctctca tcaaccgccg
     1261 ggatcacgct tggcgatccg atggcgtatc tggtggcacc gccgctggaa gcgacctacg
     1321 gcattgatgc agcgttgaaa tctgccgacg tacagctggt gacctatgtc ccgccaccgt
     1381 ctgaaaccaa ctactcggca gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct
     1441 gtaacgcctt taccgatgca gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac
     1501 ggaggttgcc gatgatcaat gcactgggat tgctggaagt ggacggcatg gtcgccgcga
     1561 tagatgcagc ggatgccatg ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc
     1621 ttgaccctgg tcgcttaacg ctggtggtgg aaggcgatct ggcggcgggt cgtgcggcgc
     1681 tggacgcagg ttgtgctgcc gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg
     1741 gtcggccaga cgatgacacc cagtggctgg ttactggctt taaccgccag ccgaagcaac
     1801 ccgtaaggga acccgacgcg ccagttatcg tcgcggaatc tgctgacgag ttgttggcgc
     1861 tgttaacatc agtacgtcag ggaatgacgg caggagaagt ggctgcccac tttggctggc
     1921 cgctggaaaa agccagaaat gcgctcgaac agctcttttc tgccgggacg ttacgtaaac
     1981 gcagtagtcg ttatcgtctc aagccccatt aacctgtcgg aggtgctggg tgtcctacaa
     2041 cacccggcat taacatcatg aaaaagaccc gtacagccaa tttgcaccat ctttatcatg
     2101 aacccttacc cgaaaacctg aagctcacgc cgaaggtcga agtggataat gttcatcaac
     2161 gacagacaac ggatgtctat gaacatgctt taacgattac cgcctggcag cagatttacg
     2221 atcagctgca tccgggcaag tttcatggtg aatttacgga aattctactc gatgatattc
     2281 aggtttttcg tgaatacacc ggtctggcgc tgcgtcagtc gtgcctggtc tggccgaact
     2341 cgttctggtt tggcattccg gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc
     2401 tgggaagcgc ggaaatcgcc acccgccctg gtggcactga atttgaactg agcacgccgg
     2461 atgattacac gatcctgggc gtggtgcttt ctgaagatgt catcacccgg caggctaact
     2521 ttttgcataa cccggatcgg gtattacata tgctgcgtag ccagtcggcg ctggaagtga
     2581 aagagcagca taaagccgcg ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg
     2641 agaacccgga aaatctccat cagcctgcag tgcgaaaagt gctgggggat aatttgctaa
     2701 tggcgatggg ggcgatgctg gaagacgcgc aaccaatggt gacggcggaa agcatcagtc
     2761 atcagagtta ccgtagatta ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg
     2821 agccggtgac ggtgctggac ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa
     2881 acgcgtttca cgctatttta ggcattggcc caaacgcgtg gctgaaacgc attcgcctga
     2941 acgccgtacg ccgcgaactg ataagcccgt ggtcgcaaag cacaacggta aaagacgccg
     3001 ccatgcagtg gggattctgg catctggggc aatttgtcac ggattaccag cagctgtttg
     3061 ccgagaagcc gtcgttgacg ttgcatcagc ggatgcggga atgggggtga gggagttaca
     3121 cccaatccct gaccttaata aactcactta acgccgcttc tgggctgcca tcttttggct
     3181 gataatcata ctcccagcgt accagtggcg gcattgacat caggatcgac tcggtgcgcc
     3241 cgccagtttg caggccaaac agagtgccgc gatcccagac cagattgaac tcgacataac
     3301 gaccgcgacg gtagagctga aaattgcgct cgcgctcgcc gtaggccatt gctttacgtc
     3361 gctctacaat tggtaaataa gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa
     3421 aacagtggtc gaaatctggc gtgttcagat catcaaagaa cagcccgcca ataccgcgct
     3481 gttcgttgcg atgtttgagg tagaagtaat cgttgcacca ctttttgtaa cggggataaa
     3541 cgtcttcacc aaatggcagg cacaggtcac gggcggtgcg gtgccagtga atggcgtctt
     3601 cttcaaaacc atagaaaggg gttaaatcga agccgccgcc aaaccaccag acgggctctg
     3661 cacccggttt ttcggcaata aaaaaacgca cattcgcgtg gctggtggga acatacgggt
     3721 tatgcggatg cactaccagt gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg
     3781 ggcgatgagc ggtggcggaa gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc
     3841 ctgcctgttc gaaaacacca ccattacgca acacccgact acgcccgccg ccgccagctt
     3901 cgcgctgcca actatcttcg acaaattctg cgccatcgac ggcggtcagc tgctgacaaa
     3961 tcgtatcctg aaggttgagc agaaactgtt taacctggtg tgcgtcgggt ttcataactt
     4021 atcgcttttt cgaatgtgct ttctggttgt cgaaccagtg gaaataactg atcacgcctt
     4081 cagcaatcgc tgtggcgatt ttctgacgaa acgccgccgt gcctaacagc cgctcttctt
     4141 ccgggttggt gataaacgag gtttccacca gcaccgaagg aaccgacggc gatttcaaca
     4201 ccacaaacgc ggcttgttcg gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt
     4261 tcagaatatg cgagccaagc gtcaggctgt ttttaatagt atcggtttgc accagatcaa
     4321 acagcacttg ctgcaatagg tgatccttgt cagtcgcctt tttaccggca acttcatcgg
     4381 cgcggttttc gcgttcagac aggtatttcg ccattgcgct acttgcccca cggttagaga
     4441 gggcaaatac cgaagcaccg gcagctttcg ggttggtaaa gccatcggca tgaattgaca
     4501 taaacagatc tgcgccatgt ttatgggcga tttcaacgcg atcgtaaagt gggataaacg
     4561 tatcgccaga acgcgttaaa cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt
     4621 ttttagcaat cgccagcacc acatgttttt ctttcgaacc gttgcgtccg atcgctccgg
     4681 tatcaattcc gccgtggcct ggatcgagaa caacgacacg tttgccgcca gattttttgg
     4741 ctttcggctt gctgtgtccg ttgctggttt ttaaaggttc gtctttggcg atggcttgcg
     4801 acattcctga caacgtcagg gcagccaaac cggctttcag cacctggcgg cgcgaagtga
     4861 gtgtttttag tggtttaaaa gtgctcatac ggcctgagtt gtaataataa agttccagat
     4921 gttatatcgt atcgcgtaat ccgttacgac ggtttgatgt tgagaattgt tttacttttc
     4981 atttcaatag ttgacaactc cccattatgc cactttttca accgattttc gtcagatatt
     5041 ggctaattct accgttcacg ccataatcag ccttttaacc cgcaaaaagg taacgaatac
     5101 catggagata cgcgtatttc gccaggaaga tttcgaagag gtcatcaccc tttgggagcg
     5161 ttgcgacttg ctgcgtccgt ggaacgatcc ggaaatggac atcgagcgta agatgaacca
     5221 tgacgtcagt ttgtttttgg tcgctgaggt gaacggtgaa gtggtcggaa cggtgatggg
     5281 cggttatgac gggcatcgcg ggtctgctta ttatcttggc gtgcatccag agtttcgtgg
     5341 gcgtgggatt gccaatgcgt tgcttaatcg gctggagaaa aagctgattg ctcgtggctg
     5401 cccgaaaatt cagatcaatg tgccggaaga taacgacatg gtgctcggaa tgtatgaacg
     5461 cctgggatat gaacacgccg acatgctgag tctgggtaag cgtttgattg aagatgaaga
     5521 gtactgagtt ccatcctgtc cattatgatg cgcacggacg cctgcgttta cccttgctct
     5581 tctggcttgt gctattgctt caggcgcgaa cctgggtgct gtttatcatt gccggtgcgt
     5641 cgcgcgagca gggtactgcg ctgctaaatc tgttttatcc cgatcacgat aatttctggc
     5701 tggggttgat tcccggtatt ccggcggtgc tggcgttttt gttgagcggt cggcgggctt
     5761 cgtttcctcg cacctggcat gtgctctatt ttctgttgct gttggcgcag gtggttttac
     5821 tttgctggca accgtggctg tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc
     5881 tggtagtggc ggatattgtg gcgttaatct ggctgctgac caatcgacgt ttacgcgctt
     5941 gttttaatga agaaaaagaa taacgcagag gtgcgctttg tttatgccgg atgcggcgtg
     6001 aacgccttat ccggcctaca aaacatcgaa aattcaatat attgcaggag aaagtaggcc
     6061 tgataagcgt agcgcatcag gcaattttgt gtttgtcatc aacctttaac ggcacttttt
     6121 ggtgaatttg cactccaagc aacgttattg aataaccaaa ggaagtgaca tgaaatcgct
     6181 gcgtttaatg ttatgcgcta tgccgttgat gctgaccggc tgttccacga tgtcgtcagt
     6241 taactggtcg gctgctaacc cgtggaactg gtttggatcg tccaccaaag tgagcgagca
     6301 gggcgtgggt gaattaacgg cgtccacacc actgcaagaa caagccattg ctgatgcgct
     6361 tgatggcgat tatcgcctgc gcagcggaat gaaaaccgcg aacggcaacg tggtgcgctt
     6421 ttttgaagtg atgaaaggcg acaacgtggc gatggtgatt aacggcgatc aaggcacgat
     6481 cagtcgcatt gacgtgctgg atagcgatat tcctgctgac accggtgtta aaatcggtac
     6541 accgtttagc gacctttaca gcaaagcatt tggcaattgc caaaaagctg atggtgatga
     6601 taatcgtgcc gtcgaatgta aagccgaagg cagtcaacat attagctacc agttcagagg
     6661 ggaatggcgt ggtcctgaag ggttaatgcc ttcggacgat accctgaaaa actggaaagt
     6721 cagtaaaatt atctggcggc gttaatttgc gtctgaacaa accgccgccg cgaaaaaaca
     6781 cgtaaaatag cgctcaacaa tgccacggat tgcgtggcat tcttattttc aggaggaaca
     6841 atgtctcagg ttcagagtgg cattttgcca gaacattgcc gcgcggcgat ttggatcgaa
     6901 gccaacgtga aaggggaagt tgacgccctg cgtgcggcca gtaaaacatt tgccgacaaa
     6961 ctggcaactt ttgaagcgaa attcccggac gcgcatcttg gtgcggtggt tgcctttggt
     7021 aacaacacct ggcgcgctct gagcggcggc gttggggcag aagagctgaa agattttccg
     7081 ggctacggta aaggccttgc gccgacgacc cagttcgatg tgttgatcca cattctttct
     7141 ctgcgtcacg acgtaaactt ctctgtcgct caggcggcga tggaagcctt tggtgactgc
     7201 attgaagtga aagaagagat ccacggcttc cgttgggtgg aagagcgtga cctgagcggc
     7261 tttgttgacg gtacagaaaa cccggcgggt gaagagacgc gccgcgaagt ggcggttatc
     7321 aaagacggcg tggatgccgg cggcagctat gtgtttgtgc agcgttggga gcacaacctg
     7381 aagcagctca accggatgag cgttcacgat caggagatga tgatcgggcg caccaaagag
     7441 gccaacgaag agattgacgg cgacgaacgt ccggaaacct ctcacctcac ccgcgttgat
     7501 ctgaaagaag atggcaaagg gctgaagatt gttcgccaga gcctgccgta cggcaccgcc
     7561 agtggcactc acggtctgta cttctgcgcc tactgcgcgc gtctgcataa cattgagcag
     7621 caactgctga gcatgtttgg cgataccgat ggtaagcgtg atgcgatgtt gcgcttcacc
     7681 aaaccggtaa ccggcggcta ttacttcgcg ccgtcgctgg acaagctgat ggcgctgtaa
     7741 gcgtttctga ttctatgatt aaaggccagc cagtaacaaa actgactggc ctttttatta
     7801 cttttgcttc aacgccgcat acacctgatc aactacccaa ccataagttg caatcggtaa
     7861 ctgaccgctt tcgaacatat tgggattttt ttgtggatcg gcaactggcg aatggggctt
     7921 gttgcttaac atcacaatcg ccatatgatt gaccggatcg ataacggtta ctgtcccggt
     7981 ccagccagtg tgaccgtagg tttgcgggct tgccagcgtg ccaaacgtcg gcgtcatggt
     8041 ggcattacca ttcacgcgcc agccgaggcc aaaagtggca tcttccttag agctggtggt
     8101 gaacatcttc accgtttccg cactgaacag ctgcacatcg ccatagcccc cgccgttcag
     8161 catcgtttgc attaacaccg caatatcgcc ggtattggaa aacaaacctg cgtgcccgga
     8221 aacgccgccc atcgaataga aggctttttc atcgtgcacc tgaccccaga gagtggaggt
     8281 gcggatattc gggaaatgga tcacgccatc gcgggtattg ccgttcaatt ccgtggcggc
     8341 aatttgttgc ggtttaaagc ctttcagcag cgggttaaat accgtatgtg tcaggccgag
     8401 cgggcgataa atcgactctt caacatagcg gtcaagcggc tgaccggtaa ccgactcgac
     8461 gataaatcca agcagcatat aatcgacatc gctgtagata tgtttgctgc cgggctgata
     8521 ttccagcggc gtgcgcttga tcatctccag cgtctggcct ttatcctagg aatataacgc
     8581 gcccgcgacc gctttattcg ggtattgcgg atctgccggg aaaccgccgc tgtgatgcag
     8641 caggtcagaa atccgcaggg tgtttttgcc tttaatggtg tcatttggac tatcggcaaa
     8701 ccccggaata tatttcgcaa tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt
     8761 ttgcagggcg aagttcgtgg cgtacatttt ggtgtttgag gccaggtcat acagcgtccc
     8821 ggtggtggct ttgacaggct gttccatcaa cacgctgcca tcgtactttt tcgccgctcc
     8881 ccaggctttg cgatacacaa tctgattatc tttaatgatc agcaggttta cgccgggata
     8941 accggcatca acttgctggc taatccagcg atccatctgg ttaagccgtt tgacgttaaa
     9001 ccctgctttc tctggcgagc tttctgtcag aacagggtat tttgcggcgc tgacgctaca
     9061 actaacagcc agcagagaaa gatagagcat tgtccgtttc atgatatcag tccagattga
     9121 cgttacggca gccaaagagc gtggtgaaaa taaacccgca aacccatgcc accagaatcc
     9181 cgccagcata aaccgccatg gccggaagga tgccttgtgc ggaagtcatc agcggcagcg
     9241 ctaccagacc agacggccca aaggcgctgt ttaagcccat cggcagaccc caccaggcta
     9301 tcaggccgat aaacaaaccg cccgccgcac cgcctaaaca ggcggtgaca aacggcttca
     9361 tgcggggcag ggtgacaccg taaatcagcg gttcaccaac gcccagcagg ccgggaataa
     9421 tcgccccgcg tacctgactg cgtagcgcac tgtgcggttg cgaccgccag tagagtgcca
     9481 gcgccgcgcc cacctggccc gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc
     9541 cctggctgtc cattaacgcg aggtaaacag gaataaagcc ctgatgcacg ccaaatacca
     9601 cggcgatcag gaacagcccg gctaaaaccg cacaaccgaa cggattactg ttcaggtgca
     9661 taaacagcca tgacatacct tcgaataacc agccacccag cggcataatg atcaggtacg
     9721 caagcgtagc ggtgatcagc agggtgatta acgaggtcag cagcatgtcg agatcgtccg
     9781 gcataaagcg gcgcaccatg ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc
     9841 cgataatatt gccgcgcgga tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt
     9901 aaccggtggt tgcagcgggg ttataaccga gcaaaaagag tgcggcaata attgcgccat
     9961 tgacgcccgt gccgccaaat gcctgggcgg cgttatagcc caccagaatc accaggaagg
    10021 tgaacaaacc tttgctgaac accttcataa aattcagcgc gtcgggtagc gtgccctgag
    10081 catctgccgg aacgtgcatc accgtggcaa ttaacgtcgc tatccccagc agcagaccgg
    10141 cggcaataaa accggggatc agcggcgtga agatggtggc gaatttggcg agaaattgtt
    10201 gcacgccgga ggtttgtttg gcttttaact gacgtttgtt ctgggcggcg atttctgcgg
    10261 catcctgtac cggtgcctcg cccagcagct cgctcatcgc ttttgcggca cgatgcgctt
    10321 taccaggtcc aaaaacgacc tgcacctggt cgctggtcaa aatgacgctc ttcacgcctt
    10381 ccagcgtttt gatattggga tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg
    10441 tcatacagtt accacaactg gcgatatttc ccggtccgcc gacacgggta agaatggtgt
    10501 tcagaagttc actgctgatc tctttggcca tgggttattc cttgtctaaa acctgacgaa
    10561 taaagccgcc gtgttgatcc aggcgttttt tagcttctgc ggcatcgaga tttttcagca
    10621 ccatcacaat ggccgtttta cagttgcgct cgcaggcaat taacgccgct tccgcttgtt
    10681 cggcattaca tccggtggcg tttttaacga tattgacctg gcgcacatgc agtttttcgt
    10741 tggtggcgac cacatcgacc ataaggttgc cgaacacttt gccggattta atcatcagcc
    10801 cggtggaaag catattgagc accagtttct gcgctgtacc tgctttcatc cgcgaagaac
    10861 cggtaacaac ttcggcacct acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg
    10921 cgctccccgg attacaggaa attcccactg tgcggcagcc gagctggcgt gcgtattcca
    10981 gtccggcaat cacatacggc gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg
    11041 ccgttaaatt aatatttttc agatcattaa caccgccttc ccggctatct tccgcgcctt
    11101 ccaccgcgtg ctgaatggca tattcgccgc cagcaatcaa accaaccacc agacccggtt
    11161 tcacgccgta ggtgggcgga cattcgctgg catccagaat ccccagacga ccggatgttc
    11221 ccgcaccgag gtaaatcaga cgcccgccgc cgcttacctg ggcgtggata acatcgatcg
    11281 ccgcggcgat atccggcagt acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt
    11341 tgataatccg gcacatttcc agcgttgata cgcggtcaat ttcagccgag gcggcgttcg
    11401 agccttcagt aatcatcttt tcaagttgca tcggtggtga ccttactatt tacgatcaaa
    11461 taattgcaga tgtatggaat catatatcct gaattgacag acttaaagga atcattgagt
    11521 ctacactcgc agagtgtgaa gagcgtcata agaacttaag cggaatttat gctgccagcc
    11581 caggagagtg gaagatgttg tacctgacga aaataagcaa tgcggggagt gaattcaccg
    11641 aaaacgagca aaaaatcgct gactttctgc aggccaacgt gagtgagctg caatccgttt
    11701 cttcacggca gatggcgaaa cagctcggca ttagccagtc gagcatagtg aaatttgccc
    11761 aaaaactcgg cgcgcaagga tttactgaat tgcgtatggc gttaataggc gaatacagtg
    11821 ccagccggga gaaaacgaat accacggcgc tgcacctgca cagttcgatt accagtgatg
    11881 attcgctgga agtgattgcc cgcaaattga atcgcgaaaa agagctggcg ctggagcaaa
    11941 cctgtgcgtt gttcgactac gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac
    12001 cgtttatcca gataacgggc ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca
    12061 aactgatgaa aattggctat cgcgtggcct gcgaagccga cacgcacgta caagccaccg
    12121 tttcccaggc gttgaaaaaa ggcgatgtac aaatcgctat ctcttacagc ggtagtaaaa
    12181 aagagattgt cttgtgcgta gaagcggcac gaaagcaggg ggcaacggtg attgccatta
    12241 cttctctggc tgactctccg ctgcggcggc tggcgcattt cacgctcgat accgtttccg
    12301 gtgaaaccga atggcgtagc tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg
    12361 atttactgtt tgttggtctt gtgcaactca atgatgtggc gtcattgaaa atgattcagc
    12421 gcagcagtga gctaacacaa cgcctgaaat gatgcataaa gcagcgactg gattgagatt
    12481 ttcctgaatt agtgagctga tccgcagcaa tattttgttt atcctgtatt ttcagaggga
    12541 atggagtgta acgctctgta ttaacaagga gagcattaaa atgggtaaac tcacgggcaa
    12601 gacagcactg attacgggcg cattgcaggg aattggcgaa ggaattgcca gaacttttgc
    12661 acgtcatggc gcgaacctaa tcttgctgga tatctcccct gagatcgaaa agctggcgga
    12721 cgaactgtgt ggtcgtggtc atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc
    12781 gtcggtagcc gcagctatca aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt
    12841 gaataacgca ggcgtttgtc gtctgggcag tttcctcgat atgagcgatg acgatcgcga
    12901 tttccatatt gatatcaata ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga
    12961 gatgattgcg cgcaaagatg gtcgcattgt gatgatgtct tcagtcactg gtgatatggt
    13021 ggccgatcct ggcgaaacgg cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa
    13081 atcgctggcg gtggagtacg cgcagtctgg tattcgcgtt aacgccattt gcccgggata
    13141 cgtgcgcaca ccaatggcgg aaagcattgc ccgccagtcg aacccggaag atccagagtc
    13201 ggtgctgact gaaatggcga aagcaatccc gatgcgtcgc ctcgccgatc cgctggaagt
    13261 cggcgaactg gcggccttcc tcgcatcgga tgaatccagc tatttaaccg gtacacagaa
    13321 tgtgattgat ggcggcagca cactgccgga gacggttagc gtcggtatct gattcacctc
    13381 tgtttcctcc ctgcatgtgt ggggaggatt tcgtcttgaa ctaagttcac caggctattt
    13441 tatttgtcat tttggccccg ggcagtgctc gaaatactca cgtactatgt gtacgctccg
    13501 gtttctccgc gctgtccgtg tccaaactgc ctgcaacaat tacgcctgtt gaaccaagtt
    13561 cttattccct ttttaacttc caaatcacca aacggtatat aaaaccgtta ctcctttcac
    13621 gtccgttata aatatgatgg ctattagaaa gtcattaaat ttataagggt gcgcaatggc
    13681 cgttaactta ctgaaaaaga actcactcgc gctggtcgct tctctgctgc tggcgggcca
    13741 tgtacaggca acggaactgc tgaacagttc ttatgacgtc tcccgcgagc tgtttgccgc
    13801 cctgaatccg ccgtttgaac agcaatgggc aaaagataac ggcggcgaca aactgacgat
    13861 aaaacaatct catgccgggt catcaaaaca ggcgctggca attttgcagg gcttaaaaac
    13921 cgacgttgtc acttataacc aggtgaccga cgtacaaatt ctgcacgaca aaggcaagct
    13981 gatcccggct gactggcagt cgcgcctgcc gaataatagc tcgccgttct actccaccat
    14041 gggcttcctg gtgcgtaagg gcaacccgaa gaatatccac gactggaacg acctggtgcg
    14101 ctccgacgtg aagctgattt tcccgaaccc gaaaacgtcg ggtaacgcgc gttataccta
    14161 tctggcggca tggggcgcag cggataaagc tgacggtggc gacaaagcca aaaccgaaca
    14221 gtttatgacc cagttcctga aaaacgttga agtgttcgat actggcggtc gtggcgcgac
    14281 caccactttt gccgagcgcg gcctgggcga tgtactgatc agcttcgagt cggaagtgaa
    14341 caacatccgt aaacagtatg aagcgcaagg ctttgaagtg gtgattccga aaaccaacat
    14401 tctggcggaa ttcccggtgg cgtgggtcga taaaaacgtg caggccaacg gtacggaaaa
    14461 agcagcaaaa gcctacctga actggctcta cagcccgcag gcgcagacca tcatcaccga
    14521 ctattactac cgcgtaaata acccggaagt catggacaaa ctgaaagata aattcccgca
    14581 gaccgagctg ttccgcgtgg aagacaaatt tggctcctgg ccggaagtga tgaaaaccca
    14641 cttcaccagc ggcggcgagt tagacaagct gttagcggcg gggcgtaact gatgtttgca
    14701 gtttcatcaa gacgcgtgct gccgggcttt accttaagcc tcggcaccag tctgctgttt
    14761 gtgtgcctga ttttgctgct gccgctctcc gcgctggtga tgcagctggc tcagatgagc
    14821 tgggcgcagt actgggaggt gatcaccaac ccacaagtgg tcgcggccta caaagtgacg
    14881 ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt tcggtctgct gatggcgtgg
    14941 attttaacac gttatcgctt tccgggccgc acgctgcttg atgcgctgat ggatttaccc
    15001 tttgcgctgc caacggcagt cgccggttta acgctggcct cgctcttttc cgtaaacggt
    15061 ttttacggtg aatggctggc gaagtttgat atcaaagtca cctatacctg gcttggtatt
    15121 gcggtggcga tggcctttac cagcattccg tttgtggtgc gtaccgtgca gccggtgctg
    15181 gaagagttag gcccagaata tgaagaagcg gcggaaacgc ttggtgcaac gcgctggcag
    15241 agtttctgca aagtggtgct gccggaactt tctccagcgc tggtggcggg cgtggcgttg
    15301 tcgtttaccc gtagtcttgg tgagtttggc gcggtgatat ttatcgccgg gaacatcgct
    15361 tggaagacgg aagtgacgtc gctgatgatt tttgtgcgct tgcaggagtt tgattacccg
    15421 gcggcgagcg cgattgcttc ggtgatcctc gcggcatccc tgctgctgct gttctcaatt
    15481 aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg gaagttaccc
    15541 aattgaagcg ttatgacgcg cgcccgatta actggggcaa atggtttctg attggcatcg
    15601 ggatgctggt atcggcgttc atcctgctgg tgccgatgat ttatatcttc gtacaggcgt
    15661 tcagcaaagg gctgatgccg gttttacaga atctggccga tccggacatg ctgcacgcca
    15721 tttggctgac ggtgatgatt gcgctgattg ccgtaccggt gaacctggtg ttcggcattc
    15781 tgctggcgtg gctggtaacg cgctttaact tccccggacg ccagttactg ctgacgctac
    15841 tggacattcc gtttgccgta tcgccggtgg ttgcaggtct ggtgtatctg ctgttctacg
    15901 gctctaacgg tccgctgggc ggctggctgg atgaacataa tttgcagatt atgttctcct
    15961 ggccgggaat ggtgctggtc accatctttg tgacctgtcc ttttgtggtg cgcgagctgg
    16021 tgccggtgat gttaagccag ggcagccagg aagacgaagc ggcgattttg cttggcgcgt
    16081 ctggctggca gatgttccgt cgcgtcacat tgccgaacat tcgctgggcg ctgctgtatg
    16141 gtgtggtgct aaccaacgcc cgcgcgattg gcgagtttgg tgcggtgtcg gtggtttccg
    16201 gctcgattcg cggcgaaaca ctttcgctgc cgttacagat tgaattgctg gagcaggact
    16261 acaacaccgt cggctccttt accgccgccg cgctgttgac gctgatggcg attatcaccc
    16321 tgtttttaaa gagtatgttg cagtggcgtc tggagaatca ggaaaaacgc gcgcagcagg
    16381 aggagcatca tgagcattga gattgccaat attaagaagt cgtttggtcg cacccaggtg
    16441 ctgaacgata tctcactgga tattccttct ggtcagatgg tcgcgctgct ggggccgtcc
    16501 ggttccggaa aaaccacgct gctgcgcatt attgccgggc tggagcatca aaccagcggg
    16561 catattcgct tccacggtac cgacgtgagc cgcctgcacg cacgcgatcg taaagtcggt
    16621 ttcgtgttcc agcattacgc gctgttccgt catatgacgg tgttcgacaa tatcgctttt
    16681 ggcctgacgg tgctgccacg tcgcgagcgc ccgaatgccg cagccatcaa agcgaaagtg
    16741 acaaaattgc tggagatggt ccagcttgcg catctggcgg atcgttatcc ggcacagctt
    16801 tccggcggcc agaaacagcg cgtggcgctg gcgcgcgcgc tggctgttga accgcaaatt
    16861 ctcctgcttg atgaaccgtt tggcgcgctg gatgcgcagg tgcgtaaaga gctgcgtcgc
    16921 tggctgcgtc aactgcatga agagctgaaa ttcaccagcg tgttcgtgac ccacgaccag
    16981 gaagaagcga ccgaagtagc tgatcgtgta gtggtgatga gccagggcaa tatcgagcag
    17041 gctgatgcgc cggaccaggt atggcgcgaa ccagcgaccc gatttgtgct cgaatttatg
    17101 ggcgaagtga accgcctgca gggaaccatt cgcggcgggc agttccatgt tggcgcacat
    17161 cgctggccgc tggggtatac gcctgcgtat caggggccgg tggatctctt cctgcgcccg
    17221 tgggaagtgg atatcagccg ccgtaccagt ctcgattcgc cgctgccggt acaggtactg
    17281 gaagccagcc cgaaaggtca ctacacccaa ttagtggtgc agccgctggg gtggtacaac
    17341 gaaccgctga cggtcgtgat gcatggcgac gatgccccgc agcgtggcga gcgtttattc
    17401 gttggtctgc aacatgcgcg gctgtataac ggcgacgagc gtatcgaaac ccgagatgag
    17461 gaacttgctc tcgcacaaag cgcctgatag gttgagtgaa tgttaaacgc ccggaggcgc
    17521 ttcccgcgat ccgggctttt taatggcaag gtttgtaacc tgtagacctg ataagacgcg
    17581 caagcgtcgc atcaggcaac accacgtatg gatagagatc gtgagtacat tagaacaaac
    17641 aataggcaat acgcctctgg taaagttgca gcgaatgggg ccgaataacg gcagtgaagt
    17701 ctggttgaaa ctggaaggca ataacccggc aggttcggtg aaagatcgtg cggcactttc
    17761 gatgatcgtc gaggcggaaa agcgcgggga aattaaaccg ggtgatgtgt taatcgaagc
    17821 caccagtggt aacaccggca ttgcgctggc aatgattgcc gcgcttaaag gctatcgcat
    17881 gaaattgctg atgcccgaca acatgagcca ggaacgccgt gcggcgatgc gtgcttatgg
    17941 tgcggaactg attcttgtca ccaaagagca gggcatggaa ggtgcgcgcg atctggcgct
    18001 ggagatggcg aatcgtggcg aaggaaagct gctcgatcag ttcaataatc ccgataaccc
    18061 ttacgcccat tacaccacta ccgggccgga aatctggcag caaaccggcg ggcgcattac
    18121 ccattttgtc tccagcatgg gaacaaccgg cactatcacc ggcgtttcac gctttatgcg
    18181 cgaacaatcc aaaccggtga ccattgtcgg cctgcagccg gaagaaggca gcagtattcc
    18241 cggcattcgc cgctggcctg cggaatatct gccggggatt ttcaacgctt ctctggtgga
    18301 tgaggtgctg gatattcatc agcgcgatgc ggaaaacacc atgcgcgaac tggcggtgcg
    18361 ggaaggaata ttctgtggcg tcagctcagg cggcgcggtt gctggagcac tgcgggtggc
    18421 aaaagctaac cctggtgcgg tggtggtagc gatcatctgc gatcgtggcg atcgctacct
    18481 ctccaccgga gtgtttgggg aagagcattt tagccagggg gcggggattt aagattattg
    18541 cgcaggcacc gttttatccg tctgcgcaat tagcgcatgt aaaaaaggct gtgcgttctc
    18601 atcgctctta ccggggatct tcacaatata cggcttcccg gtaatcgatg acgacgaagc
    18661 aaccttatca ataaactgct cggcagtgtc aatgcggtta cgggtattgc cgagcttcag
    18721 acgcagatga gaaaccgctt catcgcaggt atgttcatca ccgttgcgca caaatatcaa
    18781 atcctttttc tgtgataatc cctccagcat ggcgttgatg cgggcttctt catgggcagt
    18841 taacttcgcg taagcgggga gtgtcattag tagtgtaatg accagacaaa tgattttctt
    18901 cattgaatta ccttatcctt gttttgtaat agccgatttg actgccagaa tcaggtaaag
    18961 tttcggttgg aatacgatga ggatcatcgg caaggagaaa aaattaaatg agtagtttgt
    19021 tgttgtttaa cgataagagc agggcgctgc aggcggatat cgtcgccgtg cagtcgcagg
    19081 tggtttacgg cagcgtgggc aacagcattg ccgtgcctgc tatcaaacag aacggtctga
    19141 atgtttttgc cgtgccgacg gtattgctga gcaatacgcc gcattatgac actttctacg
    19201 gtggtgcgat tccggatgaa tggtttagcg gttatttgcg tgcgcttcag gagcgtgatg
    19261 cgctgcgcca gctgcgcgct gtaaccacgg gctatatggg aacggcatcg caaatcaaaa
    19321 tccttgccga gtggctgact gcgctacgca aagaccatcc tgacctattg atcatggtcg
    19381 atccggtgat tggcgatatt gatagcggaa tttatgtcaa acctgacctt cccgaggcgt
    19441 atcgacaata tttactgccg ctggcgcagg gaattacccc caatatcttt gagctggaaa
    19501 tcctgaccgg taaaaattgc cgcgatctcg acagtgccat tgctgccgca aaaagtctgc
    19561 tttcagacac attaaaatgg gtggtgatta ccagcgcctc cggtaatgaa gaaaatcagg
    19621 agatgcaggt ggtggtggtc agtgccgaca gcgtgaatgt catttcccat tcacgggtaa
    19681 aaaccgacct gaaagggact ggcgacctgt tttgtgctca gcttatcagt ggtttgctga
    19741 aagggaaggc gttaaacgat gcggtgcacc gtgcggggtt gcgcgtactg gaagtgatgc
    19801 gctacaccca gcagcatgag agcgatgaat tgattttgcc gccgctggcg gaagcataaa
    19861 aaatggcgcc gatgg
//
LOCUS       DAJVOY010000067        19479 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVOY010000067 DAJVOY010000000
VERSION     DAJVOY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.067"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1230
                     /locus_tag="OQL90_002078"
     CDS             <1..1230
                     /locus_tag="OQL90_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1368854.1"
                     /translation="FFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSS
                     LESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLML
                     LAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIIC
                     QAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPL
                     GIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALA
                     LAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQA
                     VNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTWH
                     DFLPSGTASVSEKHSER"
     gene            1220..2935
                     /gene="hyfG"
                     /locus_tag="OQL90_002079"
     CDS             1220..2935
                     /gene="hyfG"
                     /locus_tag="OQL90_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCS1368855.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            2945..3484
                     /gene="hyfH"
                     /locus_tag="OQL90_002080"
     CDS             2945..3484
                     /gene="hyfH"
                     /locus_tag="OQL90_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1368856.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            3481..4239
                     /gene="hyfI"
                     /locus_tag="OQL90_002081"
     CDS             3481..4239
                     /gene="hyfI"
                     /locus_tag="OQL90_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1368857.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            4232..4645
                     /gene="hyfJ"
                     /locus_tag="OQL90_002082"
     CDS             4232..4645
                     /gene="hyfJ"
                     /locus_tag="OQL90_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1368858.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            4675..6687
                     /gene="hyfR"
                     /locus_tag="OQL90_002083"
                     /pseudo
     CDS             4675..6687
                     /gene="hyfR"
                     /locus_tag="OQL90_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            6709..7554
                     /locus_tag="OQL90_002084"
                     /pseudo
     CDS             6709..7554
                     /locus_tag="OQL90_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(7592..8653)
                     /locus_tag="OQL90_002085"
     CDS             complement(7592..8653)
                     /locus_tag="OQL90_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1368859.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            8866..10329
                     /gene="bepA"
                     /locus_tag="OQL90_002086"
     CDS             8866..10329
                     /gene="bepA"
                     /locus_tag="OQL90_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1368860.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            10350..10709
                     /gene="arsC"
                     /locus_tag="OQL90_002087"
     CDS             10350..10709
                     /gene="arsC"
                     /locus_tag="OQL90_002087"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1368861.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLANSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(10847..11593)
                     /gene="hda"
                     /locus_tag="OQL90_002088"
     CDS             complement(10847..11593)
                     /gene="hda"
                     /locus_tag="OQL90_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1368862.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11643..12932)
                     /gene="uraA"
                     /locus_tag="OQL90_002089"
     CDS             complement(11643..12932)
                     /gene="uraA"
                     /locus_tag="OQL90_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1368863.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(13018..13644)
                     /gene="upp"
                     /locus_tag="OQL90_002090"
     CDS             complement(13018..13644)
                     /gene="upp"
                     /locus_tag="OQL90_002090"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1368864.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            13968..15005
                     /gene="purM"
                     /locus_tag="OQL90_002091"
     CDS             13968..15005
                     /gene="purM"
                     /locus_tag="OQL90_002091"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1368865.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            15005..15643
                     /gene="purN"
                     /locus_tag="OQL90_002092"
     CDS             15005..15643
                     /gene="purN"
                     /locus_tag="OQL90_002092"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1368866.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            15815..17881
                     /gene="ppk1"
                     /locus_tag="OQL90_002093"
     CDS             15815..17881
                     /gene="ppk1"
                     /locus_tag="OQL90_002093"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1368867.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            17886..19427
                     /gene="ppx"
                     /locus_tag="OQL90_002094"
     CDS             17886..19427
                     /gene="ppx"
                     /locus_tag="OQL90_002094"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1368868.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         4701 a         5093 c         5089 g         4596 t
ORIGIN      
        1 ttcttccatc tgtttttgtt caccatgctg ctggttgtta ccagcaataa cctgattgtg
       61 atgtgggcgg cgatcgaagc caccacctta agctcggcgt ttctggtagg catttacggt
      121 cagcgttcat cgctggaatc agcatggaag tacatcatta tttgtactgt tgatgtcgct
      181 tttggtctgt tcggtaccgt gctggtatac gccaacgccg ccagcgttat gccgcaggca
      241 gaaatggcga tattctggag cgaggttctt aagcaatcgt ccttgcttga cccaacatta
      301 atgctgttgg cctttgtgtt tgtgctaatt ggctttggta ccaaaaccgg gctatttccc
      361 atgcacgcct ggctgccgga tgctcacagt gaagcgccga gtccggtcag cgccctgctc
      421 tccgccgtat tgctgaactg cgcgctgttg gtgctgattc gctattacat cattatttgc
      481 caagccatcg gcagcgattt ccccaaccgg ttgttgctca tcttcggcat gttgtcggtt
      541 gccgtggcgg catttttcat tctggtacag cgggacatta agcgtctgct ggcgtactcc
      601 agcgtggaga acatggggct ggtcgcggtg gcgctaggca ttggcgggcc gctgggaatt
      661 tttgccgcgc tgctgcacac cttaaaccac agtctggcaa aaacgctgct gttctgcggt
      721 tccggcaatg tactgctcaa gtacggcacg cgcgatctca acgtcgtctg tgggatgctc
      781 aaaatcatgc catttaccgc cgtgctgttt ggcggcggtg cgctggcgct ggcagggatg
      841 ccgcccttca acatttttct tagcgaattt atgaccgtta ccgcaggact ggcacgtaat
      901 cacctgctga ttatcgtcct gctgttattg ctgttaacgc tggtgctggc gggcctagta
      961 cggatggctg cgcgggtgtt aatggcgaaa ccgccgcagg ccgttaaccg gggtgatctc
     1021 ggctggttga ccacctcgcc aatggtgatt ctgctggtca tgatgctggc gatgggaacg
     1081 catattccac aacctgtcat caggatcctg gcgggcgctt ccactatagt cctctcaggg
     1141 acgcacgacc tgcctgcaca acgtagcacc tggcatgatt ttttgccttc aggcaccgca
     1201 tctgtttcgg agaaacacag tgaacgttaa ttcatcgtca aatagtggcg aagcgattct
     1261 cgccgccctg aaaacgcagt tccccggcgc ggtactggat gaagagcgac aaacgcctga
     1321 acaggtcacc attacggtga aaatcaatct gctgcctgac gttgtacatt atctttatta
     1381 tcaacatgat ggctggcttc cagtcctgtt tggcaacgac gagcggacac ttaacggtca
     1441 ttacgcggtt tattatgccc tttcaatgga aggggccgaa aaatgctgga tcgtggtgaa
     1501 ggcactggtc gatgccgaca gtcgggagtt tccgtcagtc acaccgcgcg tccctgccgc
     1561 ggtctggggc gagcgagaaa ttcgcgatat gtacgggctg attccggttg gcctgccgga
     1621 tcagcgtcgc ctggtgttgc ccgatgactg gccggaagat atgcatccgc tgcacaaaga
     1681 tgcgatggat tatcgactgc gccctgaacc gacgacagat tccgaaacgt atgcgtttat
     1741 caatgagggc aacagcgatg cgcgggtgat ccctgtcggc ccgctgcata tcacctccga
     1801 tgaaccgggt cacttccgct tgtttgtgga tggcgagcaa attgtcgatg ctgattaccg
     1861 cctgttttat gtccatcgag gcatggagaa actggcagaa acgcggatgg gctacaacga
     1921 agtgaccttc ttatcggacc gcgtgtgtgg gatttgcggt tttgcccaca gtgtggccta
     1981 taccaactcg gttgaaaatg cactggggat tgaggtgccg caacgagcgc ataccattcg
     2041 ctcgattctg ctggaagtcg aacggctaca cagtcatttg ctcaaccttg gcctctcctg
     2101 ccattttgtt ggttttgata ccggctttat gcaatttttc cgcgtgcggg aaaagtcgat
     2161 gacgatggcg gaattgctga ccgggtcgcg taaaacctac ggtctgaatc tgattggtgg
     2221 tgttcgccgc gatattctca aagagcaacg tctgcaaacg ctgaaactgg tgcgcgagat
     2281 gcgcgccgac gtgtcggagc tggtagagat gctgcttgct acgccgaata tggaacaacg
     2341 cactcagggc attggcattc tcgaccgaca aatcgcccgt gattatagcc ctgtagggcc
     2401 gctgatccgc ggcagtggtt ttgcccgtga tttgcgcttt gatcacccct acgccgacta
     2461 cggcaatatt ccaaaaacac tgtttacctt caccggcggc gatgttttct cccgcgtgat
     2521 ggtccgtgtc aaagagacgt ttgattcgct ggcaatgctg gaatttgccc tcgacaacat
     2581 gccggatacc ccactgctga ccgaaggctt tagctataaa cctcacgcat tcgcgctggg
     2641 ctttgttgaa gcgccacgcg gtgaagacgt gcactggagc atgctcggtg ataaccaaaa
     2701 attgttccgc tggcgctgcc gtgccgccac ctacgccaac tggccggtgt tgcgttacat
     2761 gctgcgcggc aataccgttt ctgacgcacc gctgattatc ggtagccttg atccctgcta
     2821 ctcctgtacc gaccgtgtga cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgcc
     2881 gtataaagag atcgaacgct acggcattga tcgtaaccgt tcgccgctga agtaaggaca
     2941 gaagatgctg aagttactga aaactattat gcgcgccgga accgcgacgg tgaaatatcc
     3001 cttcgcgcca ctggaggtca gcccaggctt tcgcgggaaa ccggacctga tgcccagcca
     3061 atgtattgcc tgcggcgcct gtgcttgtcc ggcaaatgcg ctgactatcc agaccgacga
     3121 ccagcaaaat tcgcgcacct ggcagctcta tctggggcgt tgtatttact gcggacgttg
     3181 tgaagaagtg tgcccgacca gagccatcca gcttaccaat aactttgaac tgaccgtcac
     3241 caataaagcc gatctctata cccgcgcgac gttccatcta caacgttgca gccgttgcga
     3301 acgcccgttt gccccgcaaa aaaccgtcgc actggctgct gaattgttgg cacagcaaca
     3361 aaatgcgcca caaaaccgcg aaatgttgcg ggcgcaagcg agcgtctgcc cggagtgcaa
     3421 acaacgcgcg acgctgatca acgacgatac agatgtaccg ctggtggcta aggagcagct
     3481 atgagtccag tgcttacaca acatgtcagc cagcccatca cgctggatga gcaaacgcaa
     3541 aagatgaagc ggcatttgct acaggatatc cgtcgctcag cctacgttta tcgcgtcgat
     3601 tgcggcggct gcaacgcctg tgaaatcgaa atttttgctg ccatcacccc cgtattcgac
     3661 gcagaacgtt ttggcattaa ggttgtttca tcaccgcgtc acgccgatat tttgttattt
     3721 actggcgcag tcacccgggc gatgcgtatg cctgcacttc gggcgtatga gtctgccccc
     3781 gatcataaaa tctgtgtttc ctacggcgcg tgcggtgtcg gcggcggtat tttccacgat
     3841 ctctacagcg tctggggcgg cagcgacacc attgtcccca ttgatgtttg gatccccggc
     3901 tgcccgccaa caccggccgc caccattcac ggtttcgccg tggcgctcgg tttgctgcaa
     3961 cagaagattc acgctgtgga ttatcgcgat cccaccgggg tgactatgca accgttgtgg
     4021 ccacagatcc cgccatcaca gcgtatcgcc attgagcgag aagcgcggcg gctggcgggc
     4081 tatcgtcagg ggcgagaaat ttgcgatcgg ctcctgcgcc atttaagcga cgatcctacc
     4141 ggaaatcggg ttaacacctg gttgcgcgat gccgacgatc cacgtctcaa tagtatcgtt
     4201 cagcaactct ttcgcgtact ccgggggtta catgactgaa gagtgcgggg aaattgtttt
     4261 ctggacgctg cgaaaaaagt ttgtcgccag tagcgacgag atgccggaac accgctctca
     4321 ggtaatgtat tactcgctgg ctatcggcca tcacgttggc gtgattgatt gtctgaatgt
     4381 cgccttccgc tgcccactga cggaaaacga agattggctt gcacaggtcg aagaggagca
     4441 agcccgacgt aagatgctgg gggtgatgac ctttggtgag attgttattg acgccagcca
     4501 caccgccctg ttgacccggg cattcgcgct actggcagat gacgcgacgt ctgtgtggca
     4561 ggcgcgtagc attcaattca ttcatctgtt ggatgaaatt gtgcaggaac cggccatcta
     4621 tctgatggcc agaaaaattg cgtgagaagg atttctcatt aataaggact gttgatggct
     4681 atgtcagacg aggcgatgtt tgccccgcca caaggaataa caattgaagc ggtaaacgga
     4741 atgctcgcgg agcggttagc acagaaacac ggtaaggcgt ctttattacg cgccttcatc
     4801 ccgatgccgc cgccgttcag cccggtacaa cttattgaac tgcatgttat caaaagcaac
     4861 ttctattacc gctaccatga tgatggcagc gatgtgacgg caacaacaga gtatcagggc
     4921 gagatggtcg attattcgcg tcacgccgtc cttctcggca gtagtggaat ggcggagcta
     4981 cgctttattc gtacccacgg cagccgtttt actccctagg attgcacact gtttaactgg
     5041 ctggcacgca ttatcactcc ggttctgcaa tcatggctca atgatgaaga acaacaggtg
     5101 gcgctgcgtt tgctggagaa agatcgcgat catcatcggg tactggttga tattactaat
     5161 gcagtgctgt cacatcttga tctcgacgat ctgatcgctg acgtcgctcg tgagatccat
     5221 cattttttcg gtctggcttc agtcagtatg gtactgggcg agcatcgaaa gaacgagaag
     5281 tttagcctgt ggtgcagcga tctttctgcc tcacattgtg cgtgtctgcc acgcaatatg
     5341 cctggcgaca gtgtattgct gacacaaacg ctacaaaccc gacaaccgac cttgacgcac
     5401 cgtgcagacg atctgtttct ctggcaacgc gacccgttat tactcttact tgcatctaac
     5461 ggctgcgaat ctgcgctcct tataccgctt acctttggca accatacacc gggtgcattg
     5521 ttgctggcgc atacctcttc cactctcttt agtgaggaaa actgccagct actacaacac
     5581 atagccgatc gcatcgctat tgccgttggc aatgccgatg cctggcgtag catgaccgat
     5641 ttgcaggaaa gtttgcagca agaaaactac cagcttagcg agcagctcct ttcgaatctg
     5701 ggcatcggtg acattatcta tcaaagccag gcaatggaag acctgctcca gcaggtagat
     5761 attgtagcga agagcgacag tacggtgttg atttgtggtg aaaccggaac cggcaaagag
     5821 gtgatcgcca gagcgctcca tcaacttagc acgcgacgcg acaaaccgct ggtcaaaatc
     5881 aactgcgctg ccatccccgc cagtcttctg gaaagtgagt tattcggtca tgacaaaggg
     5941 gcgtttactg gtgcgattaa tacacatcgt ggtcgttttg aaattgccga tggcggcacg
     6001 ttgtttctcg atgaaattgg cgatctgccg ttagaacttc agcctaaact gctgcgcgta
     6061 ttgcaggaac gggagattga gcgtctcggc gggagtagaa cgatcccggt gaatgtcaga
     6121 gtcattgccg ccaccaaccg tgatttgtgg caaatggttg aagatcgcca gtttcgcagc
     6181 aatctctttt atcgcctgaa tgtcttccca ttggaattgc cgccgctacg cgaccgtccg
     6241 gaagatatcc ctcttttagc aaaacatttc acgcaaaaaa tggcgcgcca tatgaatcgc
     6301 gcaattgacg ccatcccgac cgaggcgcta cgccagttga tgtcgtggga ctggccgggc
     6361 aacgtgcacg agctggaaaa cgtgattgag cgagcggtac tgctgactcg cggtaacagt
     6421 ctgaatttac atcttaatgt ccgacaaagc cgtttactgc cgacgctaaa tgaagattca
     6481 gcgcttcgca gttcaatggc gcagttgctg cacccgacga cgccagagaa tgacgaagaa
     6541 gaacgtcagc gcattgttca ggtattgcga gaaaccaatg gcattgttgc cgggccccgt
     6601 ggcgcggcga cacgattagg gatgaagcgc accacgctgc tgtcacgaat gcagcgtctg
     6661 gggatctcgg ttcgcgaggt gttgtaatct gcttttgcag gagtatgcat gagaaacaaa
     6721 ctctctttcg acttgcagtt gagcgccaga aaagcggcaa tcgctgaacg gattgccgtc
     6781 cataaaattg cccgcagtaa agtgtcggtc tttttaatgg cgatgtccgc tggcgtgttt
     6841 atggcgatcg gatttacttt ttacctttcc gttatcgccg atgccccgtc ttcacaggca
     6901 ttaacccatc tggtgggcgg cctttgcttt acactcggct ttattttgct ggcggtttgc
     6961 ggcacagcct gttcacctcg tcggtaatga cggtgatagc aaaaagtcgg ggcgttatta
     7021 gttggcgaac ttggctgatt aacgcacttc tggtggcctg tggtaatctg gcagttattg
     7081 cctgtttcag tttgttaatc tggttttccg ggctggtgat gagtgaaaac gcgatgtggg
     7141 gagtcgcggt tttacactgc gccgagggca aaatgcatca tacatttact gaatctgtca
     7201 gcctcggcat tatgtgcaat ctgatggttt gcctggcgct gtggatgagt tattgcgggc
     7261 gttcgttatg cgacaaaatc gtcgccatga ttttgcccat caccctgttt gtcgccagtg
     7321 gctttgagca ctgtatcgcc aatttgtttg tgattccgtt cgccattgcc attcgccatt
     7381 tcgccccccc ccttctggca actggcgcac agtagcgcag acaattttcc ggcactgacg
     7441 gtcagccatt ttattaccgc caatctgctc ccggtgatgc tgggtaatat tatcggcggt
     7501 gcagtgctgg tgagtatgtg ttatcgggct atttatttac gtcaggaatc ctgaaaaatc
     7561 agcccggcga aacagttcgt cgggctgaag attattcttg cgcgatttgc ccatcgggcc
     7621 aggcgtggat cacggctttg atcagcgtcg ccaatggaat ggcgaagaat acgccccaga
     7681 atccccacaa accaccgaag atcaccaccg ataaaataat caccagcgga tgcaggttaa
     7741 ccgcttcgga gaacaacacc ggtactagca ggttgccgtc cagcgcctga ataatcagat
     7801 acaccgcgaa gcagctccag aattccgtgc ctgcgccaaa ctggaatagc gccacgccaa
     7861 ccaccggaat ggtcaccaca aatgcgccaa tgtacggaat aagaaccgag aagccgacca
     7921 gcaccgccag cagcagcgaa tagttcagcc caaagagcaa gaaccccagc caggtggcga
     7981 tccccaccac gatcatctcc agcactttgc cgcggatata gttggtgatt tgttgattca
     8041 tctccttcca cacctgtcct gccagtccac ggttgcgcgg cagcacccga cgaacggcgt
     8101 tcagcatctg ctctttgtct ttcagcagga agaagaccat caatggcacc agcaccagat
     8161 agacggcaat ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc accgaatcgc
     8221 ccatggtcag catccgactg cgcatatttt cggccattgc atcaataatg cccgcgtcca
     8281 ttaacgccgg atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc
     8341 ccggcatatc gcggattaag tagatgccct gttgccaggc gataggcagt accacgaacg
     8401 ccatcagtag caatataccg acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg
     8461 agcagccaat agattgcagg cgcacggttg gccattccag caaataggcc agcacaatag
     8521 ccaccagcaa cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta
     8581 aaataaccag caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca
     8641 acatttcgag cataaaaacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt
     8701 atcgaaatgt cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca
     8761 tttgagtggg ctaatcttcg ccgttacact caaaggcggc gcggtgggaa cgatatttca
     8821 cagtatcggt caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg
     8881 aaaaaaaacc tggttgcaac cctcattgct gctatgacca ttggtcaggt agccccggcg
     8941 tttgccgaca acgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc
     9001 attggtcagg aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg
     9061 ttaattaatg acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg
     9121 catgccaatt cggttaagac accgtttcat ttttttctaa tcaacaacga cgaaattaac
     9181 gcctttgctt tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat
     9241 aacgaaagtc aactggcttc agttatggcg cacgaaatct cccacctcac ccaacgtcac
     9301 ctggcgcgag cgatggaaga tcagcagcgc agcacgccgc tgacctgggt cggcgcgtta
     9361 ggttctattt tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca
     9421 ctggcgggaa cgcgtcaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac
     9481 cgcattggta ttcaggtgct gcaacgctcg ggattcgatc cgcaggcgat gccaaccttc
     9541 ctcgaaaaat tactcgatca ggcgcgttac tcctcgcgcc cgccggaaat tttattgact
     9601 cacccgttgc cggaaagtcg tctggcagat gcccgcaacc gtgctaatca gatgcgaccg
     9661 atggtggtgc agtcgtcgga agatttctat ctggcgaaag cgcgcacact ggggatgtat
     9721 aattccggac gtaaccagct caccagtgat ttgctggatg aatgggcgaa aggaaacgtt
     9781 cgtcagcaac gagcggcgca atatggtcgt gctttacagg cgatggaagc caataaatac
     9841 gacgaggcgc gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa cgcatggtat
     9901 ctcgatctgg ctaccgatat cgatcttggg caaaacaaag ccaatgaggc aatcaatcgc
     9961 ctgaaaaatg cccgtgattt gcgcaccaat ccggtgttgc agctcaacct ggcaaacgct
    10021 tatctgcaag gcggtcaacc acaagaagcg gccaatattc tcaatcgcta cacctttaat
    10081 aataaagatg acagcaacgg ctgggattta ctggcacagg cggaagccgc gctaaataac
    10141 cgcgatcagg agctggctgc gcgagcagaa ggttatgcgc tcgccggacg actcgatcag
    10201 gccatttcgc tgttgagtag cgccagttcg caggtgaaat taggcagcct gcaacaagcg
    10261 cgttacgatg cgcgcatcga ccagttgcgc cagctgcagg aacgctttaa gccttatacc
    10321 aagatgtaat tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc
    10381 cacgctgttc aaagagccgg gaaacgctga acctgctgaa agaaaacggc gtggagccag
    10441 aagtggtact ctaccttgag acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa
    10501 tgctggggat gaatagcgcc cgtgaattga tgcgccagaa agaggatctt tataaagaac
    10561 tgaacctggc taacagttcg cttagcgaag aggcgctgat tcaggcaatg gtggataatc
    10621 cgaagctgat ggagcgcccg attgtggtcg cgaatggcaa agcgcggatt ggtcgtccac
    10681 cggagcaggt actggaaatc gtgggttaag atgtgctcca tcgctagttg aagcacattg
    10741 ccggatgcga cgcttgccat gtcttatccg gcctacgaat actatggatt aacttcgcgt
    10801 agttcggata aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa
    10861 tttctttcac aaacggaatg gtcagcttac gttgtgcggt aatcgacgca cgatccaact
    10921 gatccaacgt cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc
    10981 ccacatcttc cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact
    11041 tatcttcatc agaaagtggc tgcaatttgt agatctgccc ccagtcgagt cgcgacgcga
    11101 gatccggtaa tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg
    11161 ttttgcccga ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat
    11221 cgcctgcaat acactcaatg ttgtcgatac aaaccagcga caaatgctcc ataccgtcga
    11281 gcacttccgg aacaaaccaa gtgcgtttat ccagcgggac atagcccacc gcatcgccac
    11341 gctgcgacaa ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg
    11401 cccagagata gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta
    11461 aagaggagtt atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg
    11521 gcaaagagag ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg
    11581 agaagtttac cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta
    11641 cattatttgt ctgttatatc cgcctcttct gcgtccagca ccacttcttc agggcgcagc
    11701 acactgatca gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata
    11761 ccttttaact ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc
    11821 acggaagtca ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca
    11881 cgaataccgg aagcaccgat gacaccataa agcagcagcg aaacgccgcc catcaccggc
    11941 aatgggatca tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa
    12001 atcgccgccc cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg
    12061 ttttctccgt aagtggtatt tggcgtggag ccaaagaagc cggagatcac ggtcgacagg
    12121 ccgttagcaa acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata
    12181 ttagccgtta ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga
    12241 atagtcagaa tggcaaacca ctcgaagcgc ggcgtataga gggttggcag cgcaaaccag
    12301 tgagcattaa taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtagccc
    12361 accagcacgc caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct
    12421 aaaaccgtga ccgccagggt ggtaatagag atgatgatgg ttttggagtc tggcgtttgc
    12481 ccttcagccg ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg
    12541 acggcaacga ttgcgcccat tgccgcaggt ggaaacagca cgtccagcca gccggtcccc
    12601 gctttcttca cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc
    12661 agcgcgactt cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg
    12721 gaaccaagat aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata
    12781 ccgttaaata acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt
    12841 gcaccaaaca tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt
    12901 ctttcactca ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata
    12961 ggcgctttac tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta
    13021 tttcgtacca aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc
    13081 gttcagtccc tgatcaatcg atgcggtata cagttcgacg tccgggtgcg ctttttccag
    13141 cgcagcgata ccttctggcg cagcgaccag caccagaact ttgatgctgc tgcagcccgc
    13201 ttttttcagc aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac
    13261 gatcagcgcc atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc
    13321 cagcgtttct tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacattttc
    13381 cagcacaccg tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt
    13441 acctttgatc tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt
    13501 ttcgaggtcg gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg
    13561 aaagcgcttg gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg
    13621 gtgtttgact tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaatc
    13681 gacggattat acctcctttc ttcaaggcgg caatattctt ttcgttgact ttagtcaaaa
    13741 tgataacgat ttgagataaa gttattttat attcaaatgg ttatgaaaga agatcattcc
    13801 atccgaaaac taacctttgc cctggcacaa gtcttctttc gccgcgcgtc tggggaaaag
    13861 acgtgcaaaa aggttgtgca aagcagcctc gcaaacgttt gctttccctg ttagaattgc
    13921 gccgaatttt atttttctac cgcaagtaac gcgtggggac ccaagcagtg accgataaaa
    13981 cctctcttag ctacaaagat gccggtgttg atattgacgc gggtaatgct ctggttggaa
    14041 gaatcaaagg cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct
    14101 tcggtgcgct gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg
    14161 acggcgtagg taccaagctg cgtctggcaa tggacttaaa acgtcacgac accattggta
    14221 ttgatctggt cgccatgtgc gttaatgacc tggtggtgca aggtgcagaa ccgctgtttt
    14281 tcctcgacta ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg
    14341 gcatcgcgga aggttgtctg caatcgggct gttcactggt gggtggcgaa acggcagaaa
    14401 tgccggggat gtatcacggt gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag
    14461 aaaaatcaga aatcatcgac ggctctaaag tcagcgacgg cgatgttctg attgcactcg
    14521 gttccagcgg tccgcactcg aacggctatt cgctggtgcg caaaattctt gaagtcagcg
    14581 gttgtgatcc gcaaaccacc gaacttgatg gtaagccatt agccgatcat ctgctggcac
    14641 cgacccgcat ttacgtgaag tcagtgctgg agttgattga aaaggtcgat gtgcatgcca
    14701 ttgcgcacct gaccggcggc ggcttctggg aaaacattcc acgcgtattg ccagataata
    14761 ctcaggcagt gattgatgaa tcttcctggc agtggccgga agtgttcaac tggctgcaaa
    14821 cggcaggtaa cgttgagcgc catgaaatgt atcgcacctt caactgcggc gtcgggatga
    14881 ttattgccct gcctgctccg gaagtggaca aagccctcgc cctgctcaat gccaacggtg
    14941 aaaacgcgtg gaaaatcggt atcatcaaag cctctgattc cgaacaacgc gtggttatcg
    15001 aataatgaat attgtggtgc ttatttccgg taacggaagt aatttacagg caattattga
    15061 cgcctgtaaa accaacaaaa ttaaaggcac cgtacgggca gttttcagca ataaggccga
    15121 cgcgttcggc cttgaacgcg cccgccaggc gggtattgca acgcatacgc tcatcgccag
    15181 cgcgtttgac agtcgtgaag cctatgaccg ggagttgatt catgaaatcg acatgtacgc
    15241 acccgatgtg gtcgtgctgg ctggttttat gcgcattctc agcccggcgt ttgtctccca
    15301 ctatgccggg cgtttgctga acattcaccc ttctctgctg ccgaaatatc ccggattaca
    15361 cacccatcgt caggcgctgg aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt
    15421 caccgatgaa ctggacggtg gcccggttat tttacaggcg aaagtcccgg tatttgctgg
    15481 tgatacggaa gatgacgtca ccgcccgcgt gcaaacccag gaacacgcta tttatccact
    15541 ggtgattagc tggtttgccg atggtcgtct gaaaatacac gaaaacgccg cgtggctgga
    15601 tggtcaacgt ctgccgccgc agggctacgc tgccgacgag taatgccccc gtagttaaag
    15661 cgccagctct gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt
    15721 tttccccctt ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc
    15781 agtgtcccgt gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga
    15841 aaaagagctc agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc
    15901 taacccgctg attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt
    15961 ctataaagtc cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc
    16021 caactctcat tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca
    16081 ggaattcgac ggcctctaca acgagctatt gctggagatg gcgcgcaacc agatcttcct
    16141 gattaatgaa cgccagctct ccgtcaatca acaaaactgg cttcgtcatt attttaagca
    16201 gtatctgcgt cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt
    16261 cctgaaggat gattacacct atctggcggt ggaaattatc cgtggagaca ccatccgtta
    16321 cgcgctgctg gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc
    16381 gccgcgtcga cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga
    16441 tattttcaaa ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg
    16501 cgatgccgaa tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc
    16561 cagtctcaag cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc
    16621 caatgcgctg gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt
    16681 ccccggcggt cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc
    16741 caatctggtg aacaaaccac tgccgcgttt acgccacatt tggtttgata aagcccagtt
    16801 ccgcaatggt tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac
    16861 ctttgagcat gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat
    16921 taaaattaac atttaccgcg tggcgaaaga ttcacgcatc atcgactcga tgatccacgc
    16981 cgcacataac ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga
    17041 agccaacatt cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc
    17101 gccggggctg aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt
    17161 ggtccgttac gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac
    17221 tgactattcg ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa
    17281 ctttattgaa aacccatacc gcccagtgac atttgattat ttaatggtgt caccgcaaaa
    17341 ctctcgccgt ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct
    17401 gcccagtggt atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct
    17461 gtatgcggcc tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct
    17521 gatccccaat ccggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta
    17581 ccttgaacat gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc
    17641 ttccgccgac tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct
    17701 cgatccgcac ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt
    17761 caaagcacgt tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg
    17821 caaagtacgg gcgcagttgg cgatttacga ctacatcaaa tcactcgaac aacctgaata
    17881 accctatgcc aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg
    17941 gttcaaacag ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg
    18001 gccgcctgaa acagcgagtg catctggcgg acggcctggg gccagataat atgttgagtg
    18061 aagaggcaat gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt
    18121 tttctcctgc cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca
    18181 ctgactttct gaaacgcgtg gaaaaggtca ttccctaccc gattgaaatt atttccggta
    18241 atgaagaagc ccgtctgatt tttatgggcg tggaacatac ccaaccggag aaaggtcgca
    18301 aactggttat tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac
    18361 cgattctcgt tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tacttccctg
    18421 gcggggtcat caataaagag aattttcagc gcgctcgtat ggcggcagca caaaaactgg
    18481 aaactttaac ctggcaattc cgtattcagg gctggaacgt ggcaatgggc gcttccggta
    18541 ccataaaagc cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc
    18601 cggaacgtct ggaaaaactg gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga
    18661 gtttaccggg tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat
    18721 gcggtgtgtt tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg
    18781 aaggcgtact gtacgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg
    18841 ccagcagcct cgccaaccag tatcacatcg acagcgaaca ggcccgacgg gtgctggata
    18901 ccactatgca aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac
    18961 tggaggcgct actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca
    19021 gcggtttgca tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc
    19081 aggaacagca gctgatgatg gcgacactgg tgcgctatca ccgcaaagcg attaaactcg
    19141 acgatctgcc gcgctttacc ttgtttaaga agaaacagtt cctgccactg attcagctat
    19201 tgcgccttgg cgtattactc aacaatcaac gtcaggcaac caccacaccg ccaacattga
    19261 cactgattac tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga
    19321 atgcgttggt actgcttgat ctggaaaagg agcaagaata ctgggaaggc gtggctggct
    19381 ggcggttgaa aattgaagaa gaaagtacac ctgaaatcgc cgcttaatgc tttgcgggcc
    19441 gacgagaaat gtcggcccgc attattcagg cactttcgc
//
LOCUS       DAJVOY010000068        19351 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVOY010000068 DAJVOY010000000
VERSION     DAJVOY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.068"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(23..1852)
                     /gene="glmS"
                     /locus_tag="OQL90_002095"
     CDS             complement(23..1852)
                     /gene="glmS"
                     /locus_tag="OQL90_002095"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1368869.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2014..3384)
                     /gene="glmU"
                     /locus_tag="OQL90_002096"
     CDS             complement(2014..3384)
                     /gene="glmU"
                     /locus_tag="OQL90_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1368870.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQESNTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3736..4155)
                     /gene="atpC"
                     /locus_tag="OQL90_002097"
     CDS             complement(3736..4155)
                     /gene="atpC"
                     /locus_tag="OQL90_002097"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1368871.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4176..5558)
                     /gene="atpD"
                     /locus_tag="OQL90_002098"
     CDS             complement(4176..5558)
                     /gene="atpD"
                     /locus_tag="OQL90_002098"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1368872.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5585..6448)
                     /gene="atpG"
                     /locus_tag="OQL90_002099"
     CDS             complement(5585..6448)
                     /gene="atpG"
                     /locus_tag="OQL90_002099"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1368873.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6499..8040)
                     /gene="atpA"
                     /locus_tag="OQL90_002100"
     CDS             complement(6499..8040)
                     /gene="atpA"
                     /locus_tag="OQL90_002100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1368874.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8053..8586)
                     /gene="atpH"
                     /locus_tag="OQL90_002101"
     CDS             complement(8053..8586)
                     /gene="atpH"
                     /locus_tag="OQL90_002101"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1368875.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8601..9071)
                     /gene="atpF"
                     /locus_tag="OQL90_002102"
     CDS             complement(8601..9071)
                     /gene="atpF"
                     /locus_tag="OQL90_002102"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1368876.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9133..9372)
                     /gene="atpE"
                     /locus_tag="OQL90_002103"
     CDS             complement(9133..9372)
                     /gene="atpE"
                     /locus_tag="OQL90_002103"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1368877.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9419..10234)
                     /gene="atpB"
                     /locus_tag="OQL90_002104"
     CDS             complement(9419..10234)
                     /gene="atpB"
                     /locus_tag="OQL90_002104"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1368878.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10243..10623)
                     /gene="atpI"
                     /locus_tag="OQL90_002105"
     CDS             complement(10243..10623)
                     /gene="atpI"
                     /locus_tag="OQL90_002105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1368879.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11240..11863)
                     /gene="rsmG"
                     /locus_tag="OQL90_002106"
     CDS             complement(11240..11863)
                     /gene="rsmG"
                     /locus_tag="OQL90_002106"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1368880.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11927..13816)
                     /gene="mnmG"
                     /locus_tag="OQL90_002107"
     CDS             complement(11927..13816)
                     /gene="mnmG"
                     /locus_tag="OQL90_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1368881.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14195..14638)
                     /gene="mioC"
                     /locus_tag="OQL90_002108"
     CDS             complement(14195..14638)
                     /gene="mioC"
                     /locus_tag="OQL90_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1368882.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14728..15186)
                     /gene="asnC"
                     /locus_tag="OQL90_002109"
     CDS             complement(14728..15186)
                     /gene="asnC"
                     /locus_tag="OQL90_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1368883.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            15338..16330
                     /gene="asnA"
                     /locus_tag="OQL90_002110"
     CDS             15338..16330
                     /gene="asnA"
                     /locus_tag="OQL90_002110"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1368884.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16335..17786)
                     /gene="viaA"
                     /locus_tag="OQL90_002111"
     CDS             complement(16335..17786)
                     /gene="viaA"
                     /locus_tag="OQL90_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1368885.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17780..19276)
                     /gene="ravA"
                     /locus_tag="OQL90_002112"
     CDS             complement(17780..19276)
                     /gene="ravA"
                     /locus_tag="OQL90_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1368886.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARALEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4623 a         5114 c         4846 g         4768 t
ORIGIN      
        1 ccttttttat ctatgatagt gattactcaa ccgtaaccga ttttgccaag ttacgcggct
       61 ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc cagcagctgc agcggaacgg
      121 tgtagaagat cggtgcaatc acctcttcca catgcggcat ctcgatgatg tgcatgttat
      181 cgctacttac aaaacccgca tcctgatcgg cgaagacata caactgaccg ccacgcgcgc
      241 gaacttcttc aatgttggat ttcagttttt ccaacagttc gttgttcggt gcaacgacga
      301 taaccggcat atccgcgtca atcagcgcca gcgggccgtg tttcagctcg ccagcagcgt
      361 aggcttcagc gtgaatgtag gagatctctt tcagcttcaa tgcgccttcc agtgcgattg
      421 ggtactgatc gccacggccc aggaacagtg cgtgatgttt gtcagagaaa tcttctgcca
      481 gagcttcaat gcgtttgtcc tgagatagca tctgctcaat acggctcggc agcgcctgca
      541 gaccatgcac aatgtcatgt tcaatggagg catccagacc tttcaggcga gacagcttcg
      601 ccaccagcat caacagcaca gttaactgag tggtgaatgc tttagtggac gccacgccga
      661 tttccgtacc cgcgttggtc atcagcgcca gatcggattc gcgcaccaga gaagaacccg
      721 gaacgttaca gattgccagc gaaccaaggt aacccagctc tttcgacaga cgcaggcccg
      781 ccagggtatc cgcggtttcg ccagactgtg acaaggtgat catcaggctg ttacgacgca
      841 cggcagattt acgatagcgg aattcagagg cgatttcgac gtcgcacgga atacctgcca
      901 gcgattcaaa ccagtagcgg gaaaccatac cggagttata agaagtacca caggcgagga
      961 tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt cggtcccagc tcgcttaaat
     1021 caacctgacc gtggctgatg cgcccagtaa gggtgttttt gatcgcgttc ggctgttcgt
     1081 agatctcttt ctgcatgtag tgacggtaaa tacctttatc gcccgcgtca tattgcagat
     1141 tggattcgat atcctgacgt tttacttccg cgccagtttt atcgaagatg tttaccgaac
     1201 ggcgagtaat ttccgcaata tcgccctctt caaggaagat aaagcgacgg gtcaccggca
     1261 gcagcgccag ctggtcagaa gcgataaagt tttcgcccat acccaggcca atcaccagcg
     1321 gactaccaga acgtgccgcc agcagggtat ccgggtgacg ggagtccatg atcactgtac
     1381 cgtacgcacc gcgcagctgc gggatagcac gcagaacagc ctcacgcaga gtcccgcctt
     1441 gttttagctc ccagttcacc agatgggcaa tcacttcggt gtcggtttca gaaacgaagg
     1501 tatagccacg cgcttttaac tcttcacgca gcggttcatg gttttcgatg atgccgttat
     1561 gcaccaccat aatgtgttca gaaacatgcg gatgcgcgtt agcttctgaa ggttcaccgt
     1621 gggtcgccca gcgagtgtga gcaataccgg tgccgccatg cagaggatgt tcttccgctg
     1681 cctgagccag catctggact ttaccgaggc gacgcaggcg ggtcatatga ccttccgcat
     1741 caacaacggc cagaccagca gagtcatatc cgcggtattc cagacgacgt aaaccttcaa
     1801 gaaggatttc tgctacatca cgttgcgcga tcgcgccaac aattccacac atagtttttg
     1861 attccgattt atatcgttgt cggtcaacct gtatgcccgt tttttcgggc gccccgagcc
     1921 ttgtagagag tggggttatt tttatagtta ctgcttgtgg gagggagatt atgttatctc
     1981 ctcatcccag atgaccggat tacccggcca gaattacttt ttctttaccg gacgacgcca
     2041 gccttctttc tgagtctgcg gcacacggct gatagctaat gcattttcgc cgacattacg
     2101 cgtcacagtt gtacccgcag caatggtcgc gcctttgcct actgttaccg gggccaccag
     2161 ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg gtcttaaatt tattcgcacc
     2221 atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg ttatcgccaa tttccgcatc
     2281 gcccaggtaa gtcagatgac cagctttcga gcctttaccc agacgcgctt ttttcatctc
     2341 aacaaagtta ccgacgtgtg caccttccag caactcagca ccaggacgca gacgggcaaa
     2401 cgggccaata gtacaggccg ctgccagatt cacgtcttcc acaacggtat acggactgat
     2461 ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa ccggtgccga ttttcacgcg
     2521 atgaccgaga gtcacgttgc cctcgatgat aacgttagta tcaatttcaa catcgcgccc
     2581 gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg cgcagcataa cgcctgctaa
     2641 cagcagtttt tcagcctgtt cggactggta aacgcgctcc agacgggaga gttgcaggcg
     2701 gttattcaca ccttctactt cgcttaaacg ttgcggatga acggcgacga tttcacgccc
     2761 ttcctgatac gccagcgcaa taatgtcggt gatgtagtat tcgccctgag cattattgtt
     2821 ggtcagcttc gccagccagc gtttcatatc tgcgccgtta gagatcagaa tgccggtgtt
     2881 gctctcctga atctgacgct gctcgtcggt ggcatctttg tgctcaacaa tgccggtaac
     2941 tttgccattt tcacgggtga tacgtccata accggtcgga tcatccagtt tcaccgtcag
     3001 cagaccaatg ccaccctgcg gtttagcatc acgcaggcgc tggagtgttt cgacagagat
     3061 cagcggcacg tcgccgtaga gcattaaaat gtcttcatca tcggcaaaga aaggtgcggc
     3121 ctgctgcatt gcatgacccg tacccagctg ctctgcctga agcacccagt taaggttgtc
     3181 gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg tacaccaggt gaacgtgcgc
     3241 tgcgcctaat tcattcgcag catcaatgac atgctgaacc atcgctttcc cggcaagggt
     3301 atgcagcact ttcggaagat cggaatacat gcgcgtgcct ttgcctgcgg caaggatcac
     3361 tacgctcata gcattattca acatacgcgt cctgactgta atttgagaac gaatttaaac
     3421 cgcttcacct tgaaaaaact acattttttt catcgtgaaa tggacagagg ataaattgtt
     3481 caataacgga ttatccccgc tgacgaaagt gccattttcg accatcgtca cgccttttgt
     3541 ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg tggatgtttt tgctcttatt
     3601 tcgatcaatg aataaacggg aaaacagggg tgttttatat cagcaggatc tatgtgaacg
     3661 ctcttcagga cggatcacgc gtaaaaaaaa aaccagcctg tttccagact ggcttttgtg
     3721 cttttcaagc cggtgttaca tcgctttttt ggtcaactcg ataacgcgca gctgcgcgat
     3781 cgctttggcc agttccgcag acgcctgagc gtaatctacg tcgccgtgag agctgctaat
     3841 gtgctcttca gccttacgtt tcgcttccat ggctcgcgct tcgtcgagat cctggccgcg
     3901 aattgcggtg tcggccagaa cggtcacgtt gccagactgc acttcaagaa tgccgccaga
     3961 cagatagata aactcttcgt gaccgtgctg tttcacgatg cgaatcatac caggcttaat
     4021 ggcggtgagc agcggtgcgt ggccagggta gatccccagt tcaccttcgc tacccgttac
     4081 ctggattttc tcgaccagac cagagaacat ttgttgctct gcgctgacga cgtccaggtg
     4141 gtaagtcatt gccatatcac cctccgatta aggcgttaaa gttttttggc tttttccaca
     4201 gcttcttcga tggaaccgac catgtagaac gcctgctccg gcaggtgatc gtattcgcct
     4261 tccatgatgc ctttaaagcc acggatggtg tctttcaggg agacgtattt acccggagaa
     4321 ccggtgaata cttctgccac gaagaacggc tgggacagga agcgctggat cttacgagca
     4381 cgcgctacca ccagtttgtc ttcttcagac agttcatcca tacccaggat ggcgatgatg
     4441 tctttcagtt cctgataacg ttgcaggatg gactgaacgc cacgcgcggt gtcgtagtgt
     4501 tcctgaccaa ccaccagcgg gtccagctga cggctggtgg agtccagcgg gtcaacggcc
     4561 gggtagatac ccagagacgc gatctgacgg ctcagtacca cggttgcgtc aaggtgcgca
     4621 aaggtggttg ccggagacgg gtcagtcaag tcatccgcag gtacgtatac cgcctgtacg
     4681 gaggtgatag aaccagtttt ggtggaggtg atacgttcct gcagaacgcc catctcttcc
     4741 gccagggtcg gctgataacc taccgctgaa ggcatacggc ccagcagtgc ggatacttcc
     4801 gtaccggcca gggtgtaacg atagatgttg tcaacgaaca gcagaacgtc acgaccttcg
     4861 tcacggaatt tctcagccat ggtcagaccg gtcagagcaa cgcgcagacg gtttcccggc
     4921 ggctcgttca tctggccata caccagggat actttgtcga taacgttgga gtcggtcatt
     4981 tcgtggtaga agtcgttacc ctcacgagta cgttcaccta cgcccgcaaa cacagagtaa
     5041 ccggagtgct cgatcgcgat gttacgaatg agctccatca tgtttacggt tttacctaca
     5101 cccgcaccac cgaacagacc aactttaccg cccttagcga acggacacat caggtcgata
     5161 actttgatac cggtttccag cagttcctga gagtttgaca gctcttcgta ggaaggtgct
     5221 gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc ctttcatgtc gaccggttca
     5281 cccagtacgt tcatgatacg gcccagagtc gctttaccta ccgggacttc gatcgggtgt
     5341 tcgaggtctt ttacatccag accgcgacgc agaccgtcgg aggaacccat tgcgatggta
     5401 cgcacgatac cgccgccgag ctgctgctga acttccagca ccagacgctc attaccattt
     5461 tgcacctcaa gagcatcgta cacgcgcggt acggcatcct gagggaattc gacgtcaact
     5521 acggcgccga ttacctggac aatctttcca gtagccatct taaatcctct acgaaataac
     5581 ctgtttaaac cgcggcggcc cccgagacga tctcggtgag ttcctgagta atgctggcct
     5641 gacgagcttt gttgtatacc aactgcagct ctttaatcag gctgccgcca ttgtcggtcg
     5701 cggctttcat cgccaccata cgggcggcct gctcgctggc caggttttca accacgccct
     5761 gataaacctg agattcgaca taacgacgca gcagggtatc cagcaacgcc ttcggatcgg
     5821 gttcgtacag gtaatcccag gatttatgtt tcagatcatc atcatctgat gccggtaacg
     5881 gcagcagctg gctgatggtc ggaacctgag acatggtgtt aataaatttg ttgctgacaa
     5941 tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa catcactttt accggaccga
     6001 tcagttcgga cagggaaggg ttatccccca tgccggtgac ctgggcaaca acattgccgc
     6061 ccacggagtt gaagaacgac acgcctttcg agccgatcat tgcgaggtcg cattgaacgc
     6121 ctttgtcggt ccaggtcttc atttccgcca gcagtttttt gaacaggtta atgttcaaac
     6181 caccgcacaa accacggtcg gtcgacacca ccaggtagcc cacgcgttta acgtcgcggt
     6241 cttccaggta aaggtgctta tattccagat taccatgtgc aaggtgacca atcactttgc
     6301 gcatggtttc tgcataagga cggctggccg ccatgcgatc ctgcgattta cgcattttgg
     6361 aagcggcgac catctccatc gctttagtga tcttttgcgt gttctggacg cttgcgatct
     6421 tactacgtat ctcttttgcg ccggccatga gcttctcctc aatgccttgc ggcctgccct
     6481 aaggcaagcc gccagacgtt accaggattg ggttgctttg aaggaatcga ggatgccttt
     6541 cagtttgcct tcgatttcgt cgttgtagcc accggtctgg ttgatctctt gcatcaacgg
     6601 agcgtgatca cggtcgacgt aagccagcag agcggcttcg aagctgccaa ttttcgacag
     6661 ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga accagagact gctgcgcaac
     6721 ggacatcggc gcatactgtt tctgtttcag cagttcggtc actttctgac cgtggtcaag
     6781 ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga gagaacgctg ccagttcacg
     6841 atactgtgcc agagcggtac ggataccacc ggacagtttt ttcatgatct tggtctgtgc
     6901 tgcaccacca acacgggata cggaaatacc cgggttaacc gcaggacgaa taccggcgtt
     6961 gaacaggttg gtttccagga agatctgacc atcggtaatg gagattacgt tggtcggaac
     7021 gaacgcagaa acgtcacccg cctgagtttc gataatcggc agtgcggtca gagaaccggt
     7081 tttccctttc acttcacctt tggtgaaggc ttcaacgtat tcggcgttaa cacgtgcagc
     7141 acgctccagc agacgagagt ggaggtagaa aacgtcgccc gggaatgctt cacgtcctgg
     7201 cggacgacgg agcagcaggg agatctgacg gtaagcaaca gcctgtttag acaggtcatc
     7261 gtaaatgatc agcgcatctt caccgcggtc acggaagtat tcgcccattg cgcaaccggc
     7321 atacggtgcc aggtattgca gtgcagcgga ttcagacgcg gttgctacca caacgatggt
     7381 gttagccagt gcgccgtgct cttccagttt acgtaccacg ttagaaatgg tggacgcttt
     7441 ctggccgata gcgacataga tacatttgat accggaatcg cgctggttga tgatggcatc
     7501 gatagccagt gcggttttac ctgtctgacg gtcaccgatg atcaattcac gctgaccacg
     7561 accgattggg atcatggagt caacggcttt ataaccggtc tgtaccggct gatctacgga
     7621 ctgacgttcg ataacgcccg gagcgattgc ttctacagca gagaagccgt cgtgatccag
     7681 cggaccttta ccgtcgattg gtgcacccag agtgttaacc acacggccca gcaggccacg
     7741 gccaaccgga acttccagga tacggccagt acacttaact ttcatgcctt cggcaaggtc
     7801 agcgtacgga cccataacaa ccgcacctac agagtcgcgc tcgaggttca gtgcgatagc
     7861 gtaacggtta cccggcaggg agatcatttc accctgcata caatcggcca ggccgtgaat
     7921 gcggataaca ccgtcactta cagaaacaat agtaccttcg ttgtgagctt cactcacaac
     7981 attgaactga gcaatgcgct gcttgatcag ttcgctgatt tcggtggaat tcagttgcat
     8041 gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc tcaagacgac cgcgtacgct
     8101 gccatcaatg accatatcac ccgctcggat gataacgcct gccattacag acttatcgat
     8161 tttgcaattc agcttaactt tgcgtgacag acgtttttcc atcgcagcag aaattttcgc
     8221 gagctgttgt tcactcagtg cggcagcgga aatgacgtct acctcagcgg tagcctcact
     8281 cacggcacga aggtgaataa actgctccag aacatccggg agcgcgttaa gacgaccatt
     8341 ttcagccatt acccgaatca ggttctgacc gttttcgtcc agttgctcac cacaaactgc
     8401 gataaacgac tcggcgagcg tttctggcgc aagcgcgcca gagagaagct ctgccatttg
     8461 ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc tgccagcgtt ctacactttg
     8521 gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg cgagctaccg taataaattc
     8581 agacatcagc ccctccctcc ttacagttca gcgacaagtt tatccacgat gtcgctgtta
     8641 gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc cagcaacagc caggatagca
     8701 acttgcttac gcagctcttc acgggcacgt ttacgctcgg cttcaatttc tgcctgcgcc
     8761 tgggccacga ttttagtacg ttcctgttct gcctcagctt tcgcttcgtc cagaatctgc
     8821 gagcggcgtt tgttcgcctg ctcgatgatt acctgggctt ccgctttcgc ttttttcagc
     8881 tggtcggtcg cgctggcctt tgcaaggtca aggtccttat gtgctcgttc tgcggaagca
     8941 aggccgtcag caatttcttt ttgacgtttt tcgatggctg ccattaatgg cggccatacg
     9001 tacttcatgc agaacagaac gaacaggaca aacgcgatgg cctggccgag gattgttgcg
     9061 ttaagattca cagcacaatg cctctattta gttaacgttc tgatattgct ctttaaataa
     9121 aagcaacgct tactacgcga cagcgaacat cacgtacaga cccagaccta cagcgatcat
     9181 cgggatagca tccaccagac ccataacgat aaagaactga gtacgcagca gaggaatcag
     9241 atcaggttga cgcgctgcgc cttccaagaa tttacccccg aggatgccga taccgatcgc
     9301 agcaccgatt gccgccagac ccatcatcac agcggcagcc atgtacagca gatccatatt
     9361 caggttttcc atgacagtct ccagtttgtt tcagttaaaa cgtagtagtg ttggtaaatt
     9421 aatgttcttc agacgccatc gacagataga cgatcgtcag aaccatgaag atgaaggctt
     9481 gcagcgtaat gatcaggatg tggaaaatgg cccacggcac attcaggatc cactgtgacc
     9541 accacggcaa cagaccagca atcagaatga aaatcagctc accggcatac atgttaccga
     9601 acagtcgcaa accgagtgaa actggtttgg acagcaggct taccccttca aggattaagt
     9661 tgacaggaat gaacgcccag tgattgaacg gctgcagcgt caactctttc gtgaagccgc
     9721 cgatgccttt cattttgatg ctgtagaaca gaatcaggat aaatacgccc aatgccatag
     9781 acagcgttac gttcacgtcc gcagacggaa ccacacgcag tgcaggcaga cccagtacat
     9841 gttcagcaat gtacggcagc aggtcgatag gcagtaaatc catcaggttc atcaggaata
     9901 cccagacgaa gatcgtcagg gccagcggag caatcagctt gcttttgcca tggtacatgt
     9961 ctttcacgct accattaaca aagccgatca ccagctcaat cgcggtctga aacttacctg
    10021 gcacaccgct ggtcgccttt ttggctacgc tacggaataa aaccaggaac aacagaccca
    10081 gcaccaccga gaagaacatg gagtcaatat tgattgtcca gaaggtggct ggggggtttt
    10141 gtggatccac cagcgagaat gtacgcaggt ccagctgaag gttattcagg tggtgtccta
    10201 tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc ttttaccctt tgttgttaat
    10261 tacagccggt gccagtatct gaaccaccag caccaaaacc cacgtaacga tcagcggcaa
    10321 gaataccgcc tttaaaaccg ccaacgccac caccagtaac accaacatcg ccagaacttt
    10381 gaaagcttcg ccaaatgcga atgtccaggc cacccggcct ttcgctggtg tatgcgcctg
    10441 gtgacgccag gcaaatatca taaacaaaac gttaggcaga aagactgcca ggcccccgct
    10501 tattgcagag acgccccaga aggggtcttt gaggctgaac agcaatccac ttgctatcac
    10561 caccagtaac tgaacgagca gaagcttccg agcaacgttt cgactcacga gcgacacaga
    10621 catcacgttt tttactcctg ctcccttcga ggtatgccgc gtgtcgtata aaactttctt
    10681 taaggcttag agtcaagcat caaaaagcgg tcaaattata cggcgcgccc ccgtgatttc
    10741 aaacaataag tagccaaaag gtgaataaat gtttaaatat ttttccagtg catacaattg
    10801 cgacttttct actaaccctg ttcgatcatg aaaaactgta aataacgcgt aaacactggt
    10861 gataaagcgt gcttcagatc acatattgcg catgttcgcg cacagcatat ttatttactt
    10921 ggcaaatgat gcctttgcaa gtttatgata tttcagtcta aaaacagata ctgttttaat
    10981 aaatgacatt tacacaacaa aaaccaccca ttgacatttt taataatgtt ttagcagcca
    11041 atgatggttc ttagcgccga tttttagcag actgatattt tcactaatga cttattttct
    11101 gcttaccaaa aaaagccacg ttatcttgtt gatgcaaaag agtgaacgtg gcgttaaatg
    11161 taaccagtta tatcagtaga aaacctggtt gttgttaaca gtctaaccgg tcaatttttt
    11221 atgatttttt tgataaaaat taaattttat ttgctttaat caccaccaga tgacgttcgc
    11281 catccagggc tggaacctga agtttaacca ctgattcgac ctgatattct tcgggcaaca
    11341 aagcgatttc atcttccggc atttgccctt tcagcgcgta gaaacggcct tgctcaccag
    11401 gaagatggtg gcaccagctc accatatcgt tcagagaggc aaaagcgcgg ctaattacgc
    11461 catcaaatgg cggctctgaa ggaaactctt ctaccctgct ctgtacaggt tcaatattct
    11521 ccagtttaag ctcatgttgc acctgacgaa ggaaacgcac gcgtttacca aggctatcca
    11581 acagagtgaa atgggcttca ggacgcacga tagagagtgg aatgcctggc agtcctggtc
    11641 cggtgccgac atcgataaac cgttcacctt gcagatacgg tgccaccaca atgctatcga
    11701 gaatatggcg taccagcatc tcattaggat cgcggaccga agtcaggttg tacgctttgt
    11761 tccatttatg cagcatattc acgtaggcaa taagctggtt tttctggtga tcggtaagcg
    11821 aaatacctgc gtctttcagc agtaaggaga gtttgttgag cacggtgatt acctgttctt
    11881 gatgcgttgc ctggtaagcg ggtgcttacc aggcattttt aatgcgttat gcgctacgac
    11941 gcagcatacc ctgttttttc agccacacca gcagaatgga gatagccgca ggcgtgacgc
    12001 cagaaatacg cgaagcttgg ccgatagagg ctggtttgtg atcgttaagt ttggcgatca
    12061 cttcgttaga aagaccggat acctggcggt aatccagtgt cgcgggtagc agggtgttct
    12121 cgttacgcag ctgcttttcg atctcatctt gctggcgcgc gatataacct tcgtatttaa
    12181 cctgaatctc aacctgttcc gccgcctgtt cgtctgtcaa cgcaggggca aacggcgtca
    12241 gcgtggttaa tttttcataa gtcatttccg gacgacgcag cagatcttca ccactggctt
    12301 cacgggaaag cggcgcagtc aggtgagcat tcacttcggc tgcagcttcc gccgacgggg
    12361 ttacccaggt cgatttcaga cgctgacgct cacgctcgat attctcaagt ttctcgttaa
    12421 agcgcgccca acgttcgtca tccaccaggc ccagttcacg accgatttca gtcaaacgca
    12481 gatccgcatt atcttcgcgt agcatcagac gatattctgc gcgcgaagta aacatacgat
    12541 acggttcttt ggttcctaaa gtgcacaggt catcaactag tacgccgaga tacgcctgag
    12601 aacgtgccgg agcccaacct tctttgtcag cagacagacg ggcagcgtta agaccggcca
    12661 gcaaaccttg cgcagcggct tcttcgtaac cggtagtgcc gttaatctga ccagcaaaga
    12721 acagcccctg gataaacttg ctctccagcg tcggtttcag gtcgcgagga tcgaagaagt
    12781 catactcaat ggcataaccc ggacgcacga tcttcgcgtt ttccatcccc tgcatagagc
    12841 ggacgatttg catctgcaca tcgaacggca ggctggtgga gataccgttc ggataaattt
    12901 cattagaggt cagtccttcc ggttcaagga agatctgatg ctgatttctg tcggcgaagc
    12961 gcatgacttt gtcttcgatc gacgggcagt agcgtgggcc gacaccttcg atcacccctg
    13021 catacattgg gctacgatcg aggttactgc ggatcacatc atgggttttc tcgttggtat
    13081 gagtgatata acacggcacc tgctggggat gctgggacgc attgcccata aacgagaata
    13141 ccggcattgg gttatcgcca tgctgttgcg ccagcacact aaagtcgata gttcgagcat
    13201 caatacgcgg tggtgtcccg gttttcagac gaccaacgcg cagcggcagt tcacgcaaac
    13261 ggcgagaaag cggaatggac ggcggatcac cagcacggcc accgctgtaa ttatccagac
    13321 cgatatgaat tttaccgtcg aggaacgtcc caacggtgag cacgactgct ttggcacgga
    13381 acttcagtcc catttgggta acagcaccga ccacgcgatc gttttcgaca ataagatctt
    13441 caaccgcctg ctggaagatc atcaggttcg gttggttctc cagcgccgta cgtaccgcct
    13501 gacggtagag cacacgatcc gcctgagctc gggtagcgcg aaccgccggt cctttgcttg
    13561 cgtttagtat cctaaactgg atacccgctt gatcgatcgc tttcgccatc agaccgccga
    13621 gtgcatccac ttcttttacc agatgtccct tcccaatacc gccgatcgcc gggttgcagc
    13681 tcatctgccc cagagtgtcg atattgtgtg tcaaaagcag agtctgttga cccatacgcg
    13741 ccgcggccat cgcggcctcg gtgcctgcat gacccccgcc aatgatgatg acgtcaaaag
    13801 gatccggata aaacatggtg attgcctcgc ataacgcggt atgaaaatgg attgaagccc
    13861 gggccgtgga ttctactcaa ctttgtcggc ttgagaaaga cctgggatcc tgggtattaa
    13921 aaaggagatc tatttattta gagatctgtt ctattgtgat ctcttattag gatcgcactg
    13981 ccctgtggat aacaaggatc cggcttttaa gatcaacaac ctggaaagga tcattaactg
    14041 tgaatgatcg gtgatcctgg accgtataag ctgggatcag aatgaggggt tatacacaac
    14101 tcaaaaactg aacaaccgtt gttccttgga taactaccgg ttgaaccaag cttcctgaca
    14161 gagttatcca cagtagaacg cacgatctgt atacttattt gagtaaatta atccacgatc
    14221 ccagccattc ttctgccgga tcttccggaa tgtcgtgatc aagaatgttg atcttcagtg
    14281 tttcgcctgt ctgttttgca ccggaatttt tgagttctgc ctcgagttta tcgatagccc
    14341 cacaaaaggt gtcatattca cgactaccaa taccgattgc gccaaagcgg accgcagaaa
    14401 gatcgggctt ctgttcctgc aatgcttcat agaaaggaga aaggttgtcc ggaatatctc
    14461 cggcaccgtg ggtggagctg ataatcagcc agatccccga ggcagataaa tcttccaaca
    14521 gcggaccgtg cagcgtttcg gtggtaaaac ccgcctcttc cagcttttca gccaggtgtt
    14581 ctgctacata ttcggcaccg ccgagggtgc tgccgctgat aagagtgata tctgccataa
    14641 accgccacct ttattaagag tggcgtattg tacgctgtga acgcgttggg atctacctgt
    14701 ggaaaagtat gggattaaaa aagccgatca gggcttgata gtacgcatga tcgggtgttg
    14761 caggacgatc aatgtctcgg tggactgaat ttcatcaatt gtttggatct tgttgataag
    14821 tacatgctgg agagcgtcga tcgaacggca cattactttt ataaagatgc tgtagtggcc
    14881 tgttgtgtaa taggcttcag tgacttcatc aaggctttcc agctttgcca gcgcggaagg
    14941 gtagtctttg gcgctcttta atataatgcc gataaagcag cctacgtcat aaccgagctg
    15001 cttcgggctg acatcaatac gcgccccggt aatgatcccc gcctgcttca ttttctctac
    15061 ccgaacgtga atcgtccccg gactgacgcc aaattgtttc gccagttcgg cgtaagcggt
    15121 gcgcgcattg cccattaatg cttccaggat gccacggtcc agattgtcga tcagataatt
    15181 ttccatagga ttttcttatg cagattgatg attcattcta ttttagcctt cttttttaat
    15241 gaatcaaaag tgtgttaggc tttttattga atgattattg catgtgtgtc ggtttttgtt
    15301 gcttaatcat aagcaacagg acgcaggagt ataaaaaatg aaaaccgctt acattgccaa
    15361 acaacgccaa attagcttcg tgaaatctca cttttctcgt caactggaag aacgtctggg
    15421 gctgatcgaa gtccaggcac cgattcttag ccgtgtgggg gatggcacgc aggataactt
    15481 gtcgggctgt gaaaaagcgg tgcaggtaaa agtgaaagct ctgcctgatg cccagttcga
    15541 agtggttcat tcactggcga agtggaaacg tcagacctta gggcaacacg acttcagcgc
    15601 gggcgaaggg ctgtacacgc acatgaaagc ccttcgcccc gatgaagacc gtctttctcc
    15661 gttgcactcg gtctatgtag accagtggga ctgggaacgc gtaatgggcg acggtgagcg
    15721 tcaattctcg actctgaaaa gcacggtaga ggcgatctgg gcgggaatta aagcaaccga
    15781 agctgcggtt agcgaagagt ttggcctggc accgttcctg ccggatcaga tccacttcgt
    15841 acacagccag gagttactgt ctcgttatct ggatctcgat gccaaagggc gtgagcgggc
    15901 gatagcgaaa gatcttggcg cggtattcct tgtcgggatt ggcggcaagc tgagcgatgg
    15961 tcatcgccac gacgtgcgcg caccggatta tgatgactgg agcaccccgt cagagctggg
    16021 tcatgcgggt ctgaacggcg atattctggt gtggaaccct gtactggaag atgcgtttga
    16081 gctttcttcc atggggatcc gcgtggatgc cgacacgctg aagcatcagc tggcgctgac
    16141 cggtgatgaa gatcgtctgc agctggaatg gcatcaggcg ctgctgcgcg gtgaaatgcc
    16201 gcagaccatc ggcggcggta tcggccagtc tcgtttgact atgctgctgc tgcaactgcc
    16261 gcatatcggc caggttcagt gtggagtatg gccagctgct gttcgcgaga gcgtcccttc
    16321 tctgctgtaa taatttatcg ccgccagcgt ctgagcaggc ggcttcgcat cccggtatca
    16381 aagcgccaga tatgatcgaa aatgcgcatg atgccgggtt tgccgtgtgc cgacatcgcc
    16441 acggcatgaa agcgatgctg atgtacccgc tgcagctctt tcactttact cgtcacgtcg
    16501 tcaggcaacc gctgagcgat aaaatcagaa atcaccaccg catcggcatc aaaccattcc
    16561 ctgctttgca agcgttccat aatggcgcga aaacaactgg caagatcggt gccgccacga
    16621 aactgctggc ttaaaaaacg gattgcttgt tcgatgcctt gtgggcctga aagctcataa
    16681 cggacgatct cggtggaaaa tagcataata tagcagcgcc ggttttctgc gagagcaatg
    16741 cgcatcaagg ccaggcagaa cgctttcgca cactgttcat taaagccgcc cattgagccg
    16801 gaagtatcca cacagacaat aaacggcccg cgcggctgtt catcgtaatc tttatgtacc
    16861 accggacgtt cgatcacttt ttcacgccac gactcaccgt gcaggcgata ggtgagcaac
    16921 tgtttttcca ccagccgacg gtaaaactca tactccagtt ccgttatccc tagtgtcgcc
    16981 agttctggcg gcaggagacg taaaatatca tcgctttgtt gcagaccatc aacctgctca
    17041 ggaaccgtcg ccggttcgcg caccatggtg cggaaggttt ccatctgcgc atcgttgcgc
    17101 ggtattgatt tggcttcccg agaacgcccc agctgctctg ccaggcgttt cagttccggc
    17161 tgttcgttaa gaaattcacc gtatttcaca atcaactgat agtcgccacg tttaagctgg
    17221 ccggcgctca tatcccacag acgaccagct gcggtattgt tatctgcgag aatcggttca
    17281 agttgtccgc tcagcgtcat gcgttcctga acttcactta acagttgttc gcgttcttct
    17341 tctaatagct gttgatttaa cgtcgttgct tgcacgatca gacttaaacg ccagcgctgg
    17401 agaaaaagcg tgtgtaacgc tgaagttatt gtgctgttag catcaaccaa ctgccgggct
    17461 tgttctgccc atggggaatt cagacgatgc agtaagtcca ggatctgtgg tagctgcaca
    17521 ataaactgcg gcgtggagag gagctggctt tgctgatagc acatcacctc ttcggtgagt
    17581 tctggcggga ctcgggcatc tttcagccga ctgcgcagcg cctcacgcca gcggggaaca
    17641 tcatcagtga ttgctgcctt cagtcgtggg aatttttcaa agaagaccgc cagctgcggt
    17701 gaggccagca gtgcgatgat catctcttcg atcaatccct cttcgctgac ggccagcatc
    17761 acattaagcg tatccagcgt tagcattgtt gtgcctggcg aatctgtgca ccgacatcct
    17821 gtaggctggc ttcaatgcga cctaaccagt cgccagggat aaacagacat ttttgctgtt
    17881 cgctgaataa agcgtgttgc ttacgccagt cgctttcaag ctcttccagt tgttgtttta
    17941 tttcacctgg cagaccttca gccgatgaac cgggaagtgc cagcgtactg ccttgtaaac
    18001 ttacatcgcg tacaacaaga tgttgggtgc tatcaacttc cagattcagt ttctgggcaa
    18061 agccgatacc gttcagtttg ccgcgaattt caccaccttt actcagccac tgttccagcg
    18121 cgctacgctc aaaggagata tgaaccactt ccatatcatg cagttttaac ggtttttgca
    18181 gcagcagagt cagagtggaa gcagtaacgt taacagggag ttgatactgc tgacgacggc
    18241 taaaaatgcc gcccagacga attaccgtta aggctgtttt atcgctttgt tgctgctgaa
    18301 gttgcaggtg acgttgcaca atcgcgccta ggcgggtcaa catcccttgc tgttgccagg
    18361 cgtgaccggt catcaataca tcaatttgtt gttgtatcaa attcaggctt tgcgcgtcat
    18421 accacaggca atctttcagc aaaatgagat ctaccggggc aacagcactg cgaccgctaa
    18481 aaaaggcgct ggcctgcaat aatcggatcg cttttttcca gcgacgatcc gagacataag
    18541 gcgcatccgg taatttatcc agttgctggc gcagcataaa aatcagctca aatacatgat
    18601 cgggcagcgt aatttcacca atctctttct gccagcgttc atattcttca tctgtgacct
    18661 gcaaggcatc aggaaccgga ttgtcgtttt catcctgttg actggtcagc atggagcgga
    18721 aattcgcttt atcctgcact ttatctaacc acagacgaat cagcatgcgg tcatataacg
    18781 cttccagact gctgtctgct tccggcagct cgttggaggc cgccaccagc agacgcatcg
    18841 ggattttttc tacgtgtgca ccgttgcgga actggcgctc gttaatggcg gtgagcaagg
    18901 tattaagaat tgccgggccc gctttccaga tctcatccag aaagacaatt tctgcttccg
    18961 gcaggtaacc gctggttaaa cgttcatagc gcccttcatc ttttagcgcc tgaatagaaa
    19021 ggggaccaaa aacttcttcc ggcgtggaga agcgggtcat cagatattca agcgcgcggg
    19081 cattctgaaa ggcgaatttt aagcgccggg cgatcaaact tttggcaata cctggcgggc
    19141 caaggaggaa cacactttca ccacttaatg ccgctaataa acacaagcgg atggcgtggc
    19201 tacgttcata aagccccttt tccaacgaac tgctcaggcg ggaaattctt tccgctaata
    19261 aatgagggtg agccataatg aagtggcgtc ctttcgtcaa aagttctgcg taaattgcga
    19321 gtatagacgt ttcttgctgg tggctaaaat a
//
LOCUS       DAJVOY010000069        19122 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVOY010000069 DAJVOY010000000
VERSION     DAJVOY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.069"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..798)
                     /locus_tag="OQL90_002113"
     CDS             complement(<1..798)
                     /locus_tag="OQL90_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCS1368887.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAA"
     gene            1141..1971
                     /locus_tag="OQL90_002114"
     CDS             1141..1971
                     /locus_tag="OQL90_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1368888.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            2003..2938
                     /locus_tag="OQL90_002115"
     CDS             2003..2938
                     /locus_tag="OQL90_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1368889.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            3031..3273
                     /locus_tag="OQL90_002116"
     CDS             3031..3273
                     /locus_tag="OQL90_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1368890.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            3263..4714
                     /gene="panF"
                     /locus_tag="OQL90_002117"
     CDS             3263..4714
                     /gene="panF"
                     /locus_tag="OQL90_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1368891.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFY
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            4726..5607
                     /gene="prmA"
                     /locus_tag="OQL90_002118"
     CDS             4726..5607
                     /gene="prmA"
                     /locus_tag="OQL90_002118"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1368892.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            5936..6901
                     /gene="dusB"
                     /locus_tag="OQL90_002119"
     CDS             5936..6901
                     /gene="dusB"
                     /locus_tag="OQL90_002119"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1368893.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            6927..7223
                     /gene="fis"
                     /locus_tag="OQL90_002120"
     CDS             6927..7223
                     /gene="fis"
                     /locus_tag="OQL90_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1368894.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            7309..8193
                     /gene="yhdJ"
                     /locus_tag="OQL90_002121"
     CDS             7309..8193
                     /gene="yhdJ"
                     /locus_tag="OQL90_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1368895.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            8277..8456
                     /locus_tag="OQL90_002122"
     CDS             8277..8456
                     /locus_tag="OQL90_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS1368896.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(8459..9121)
                     /gene="acrS"
                     /locus_tag="OQL90_002123"
     CDS             complement(8459..9121)
                     /gene="acrS"
                     /locus_tag="OQL90_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCS1368897.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            9520..10677
                     /gene="acrE"
                     /locus_tag="OQL90_002124"
     CDS             9520..10677
                     /gene="acrE"
                     /locus_tag="OQL90_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCS1368898.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10689..13793
                     /gene="acrF"
                     /locus_tag="OQL90_002125"
     CDS             10689..13793
                     /gene="acrF"
                     /locus_tag="OQL90_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCS1368899.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            14046..14267
                     /locus_tag="OQL90_002126"
     CDS             14046..14267
                     /locus_tag="OQL90_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368900.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIAMSK"
     gene            14698..15747
                     /locus_tag="OQL90_002127"
     CDS             14698..15747
                     /locus_tag="OQL90_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1368901.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTAKERFTALQ
                     SGEVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQ
                     AGTDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLS
                     NPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAAN
                     PATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQN
                     NLWNNGGIQYAPPVR"
     gene            15815..16985
                     /locus_tag="OQL90_002128"
                     /pseudo
     CDS             15815..16985
                     /locus_tag="OQL90_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            16995..18098
                     /locus_tag="OQL90_002129"
     CDS             16995..18098
                     /locus_tag="OQL90_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1368902.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            18106..18863
                     /locus_tag="OQL90_002130"
                     /pseudo
     CDS             18106..18863
                     /locus_tag="OQL90_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
BASE COUNT         4679 a         4560 c         4817 g         5066 t
ORIGIN      
        1 ggcggccttt gcgggctgca tatagtggcg tggatcattt gccgcaggat tgttgtagaa
       61 atatcctttt aacgcatcgg caaacgcaat tttcaattct gtcgcgacat tcactttaca
      121 aataccgcgg cgaatacagt gctgaacgtc ctgttctgat aagcccgaag caccatgcaa
      181 caccagtgga atctccactc gctgacgaat ctgttccagc cgcacaaaat ccagtcgtgg
      241 ttcatgcgca tataaaccat gtgctgtgcc gattgctacg gccagggagt caatttgggt
      301 acgttgaaca aactctgcag ctgcggctgg tgaggtataa gcggaatctt tttcatccac
      361 cagcagatcg tcctcctggc cgccaagacg ccctagctcg gcctcaacgc taacatcaaa
      421 gcgatggcaa aaggtggtaa cttcttgcac tcgcgcaatg ttgtcttcaa atggcaaatg
      481 ggagccatca atcatgacag aacggatccc tgcgcgaacc ttgttaaaaa tgtcatcact
      541 ctcttcatga tggtcgagat gcaggactaa cggcaggttg tactcgcgag ccagcgattg
      601 tactaacgaa attaaacttc ccacgccacc ataacgataa gtaccgggtg ttcccgccag
      661 cataacggga gagcgaagtt gtgcagcgct ctcaacaact acctgtaaag tttcaaggtt
      721 atgaatgttg aaagcgggga cggcatatcc ctctgactgg gcttttttca gcatgttttt
      781 gcttgaaatg atgtacatcg cagctcctgt tatttgtcgt tcgccttaca aaatgaagaa
      841 agagaatatt cactttcatt ttgaaagcaa actagcacag caaaagcggc aaaaagcgtg
      901 atacatgtcg aatatcatca aaataactta caaaaaaata acttaatttg tttttcactt
      961 tacaagtgaa accaataaca tctctgcata aatatgaata aattcattta tttttcaatg
     1021 aattaatcta tttttgttgc gatttttgac ccatgatttt cttttcgaat tgtcgatatt
     1081 attgaaggat aactttcaaa gaaaaataca aggggctata gtgttacgca ttaaaggcca
     1141 atgaagagtg ttaacgtgcc gagaaaatcg tctaacctac tgaaacgcag attaaaaatt
     1201 gcagaaattg ttgccgccca aggtgaaatc aaagttgatg acttatctgc gcaacttggt
     1261 gtctctggcg tgacgatccg cggcgatctc agttatctgg agcaacaggg atatttaaag
     1321 cgatcatttg gcggtgctat tgcgacacca ctctcctctg aaccagaagc gatacaaccg
     1381 atcgcgccat tgtctctggc acaacaaatg gaaatagcac gacattgcgc acggttaatt
     1441 cgcgatcgtg acacgctatt tcttggacat gggacaatct gccgtaagat cattccattg
     1501 ctcagcgaag tgaaaaaact gcgtctgatc accaacgatc ccgaacatgc gctgctggca
     1561 aatcagttta ttgatggcga aataatactg gctggcagtg aaatgctgcg ccccgacacc
     1621 gcactggccg gaaaaccgct tgaacaggca cttcagcatt tcaccattac acatagcatt
     1681 ctggaaatca accatctgga tgctgatggt actttaaata tcaacgttcc cagactggcc
     1741 gctgcctggc agctctgttt cgatcatgcc cagaataaga cggttatcgt tgccgccgat
     1801 cctgcgccat caacggctgc tatcgggcat ttgcatcagg cagaaaatgt gatcgtcagc
     1861 cgcagcatta acgaacaata tcagcaacaa ttgcagtcgg ctgatttcgt tatcctttat
     1921 accagtaatg agtgctttac ctggtccaat cgcgaaaggc ttcaggaatg agaggggttt
     1981 tgtgacaata actatctgcg ctatgggcga attgctggcc gagtttttgt cccgcaaccc
     2041 acatcaaaaa ttcactcagc ctggggagtt tatcgggcca tttcccagcg gtgcgccagc
     2101 aatttttgct gctcaggtgg caaaactgtc ccatcgggcc atcttcttcg gatgtgttgg
     2161 taatgatgat tttggccgac tcattataga gcgcctccgt catgaaggtg tcattaccga
     2221 tgggatccat gttatgaaca atgccgtcac aggtacggcg ttcgtgagtt atcaaaatcc
     2281 ccagcagcgc gatttcgtct ttaatatccc taacagcgcc tgcggcttgt ttactgctga
     2341 gcacattgat aaggatctgc taaaacagtg taaccatctc catattgtgg gctcatcatt
     2401 gttctcattt cgcatgatcg atgtcatgcg taaagcaata acgacgatca aatcggctgg
     2461 cggcaccgtt tctttcgatc ccaatattcg caaagagatg ctgagcattc ctgaaatggc
     2521 gcaggctctc gattatttga ttgaatatac ggatattttc atccccagcg aaagcgaact
     2581 ccctttcttc gcgcgtcaca aaaatctgtc agaagaacag attgttagcg atcttctcca
     2641 cggcggcgta aaacatgtgg cgataaaacg cgcccagcgt ggggccagct attacaagct
     2701 taaaaacggt acattacacg cccagcatgt tacaggccac gatatcgaaa ttatcgatcc
     2761 aacgggtgca ggcgactgct ttggcgcaac gtttatcact cttttcttat ccggtttccc
     2821 ggcacataag gcactgcaat atgcaaatgc cagcggcgcg ctcgccgtaa tgcggcaagg
     2881 tccgatggaa gggatatcct cactggctga cattgaagac tttttgcagc agcactgaac
     2941 ggcaattttc tgtcagatta ttttccctgc ctgaagagtt gccgtagaat tgcccgtttc
     3001 tcgtatcccc ccatgaggag gaacccttcc gtggacactc gttttgttca ggcccataaa
     3061 gaggcgcgct gggcgctggg gctgaccctt ttgtatctgg cagtttggtt agcagccgct
     3121 tacttacctg acgttgcccc cggttttacc ggctttccgc gctggtttga gatggcctgc
     3181 atcctgacgc cgctgctgtt tattggactg tgctgggcga tggtgaaatt tatctatcgc
     3241 gatatcccac tggaggatga cgatgcagct tgaagtgatt ctaccgctgg tcgcctatct
     3301 ggtggtggtg ttcggtatct ctgtttatgc gatgcgtaaa cggagcaccg gtaccttcct
     3361 taatgagtat ttcctcggca gccgctctat gggcggtatt gtgctggcga tgacgctcac
     3421 cgcgacctat atcagtgcaa gttcgtttat cggcgggcct ggagctgctt ataaatacgg
     3481 cctgggttgg gtattgctgg cgatgattca gcttcctgca gtctggcttt cgctcggtat
     3541 tctcggcaag aagttcgcga ttcttgcgcg ccgctacaat gcagtgacgc tgaacgacat
     3601 gctgtttgcc cgctaccaga gtcgtcttct ggtgtggctg gcgagtttga gtttgctggt
     3661 tgcgttcgtt ggggcaatga ccgtgcagtt tatcggcggt gcgcgcctgc tggaaaccgc
     3721 ggcgggtatt ccttatgaaa ccgggctgct gattttcggt atcagcattg cgttgtatac
     3781 cgcctttggc ggctttcgcg ccagcgtgct gaacgacacc atgcaagggc ttgtgatgct
     3841 gattggcacc gttgtgctgc ttattggcgt ggtacatgcc gcaggtggtt taagtaatgc
     3901 ggtagagacg ctgcaaacca tcgatccgca actggttacg ccacaaggcg ctgacgatat
     3961 tctgtcgcct gcctttatga cgtcgttctg ggtactggtg tgctttggcg tgattggcct
     4021 gccgcatact gcggtgcgct gtatctctta taaagacagc aaagcggtac accgggggat
     4081 catcatcggt acgattgtag tcgcaatcct gatgttcggt atgcacctgg ccggagcgtt
     4141 aggtcgtgca gtgatccccg atctcaccgt accggatctg gtgatcccga cgttaatggt
     4201 aaaagtgctg ccaccgtttg ctgccgggat ctttctggct gcacctatgg ctgcgatcat
     4261 gtcgacaatt aacgcccaat tgctacaaag ttccgctacg atcattaaag atctctatct
     4321 gaatatccgt ccggatcaaa tgcaaaacga gacgcgtctg aagcggatgt cggcggtaat
     4381 tacgttagtt ctcggcgcgt tgctgctgct tgccgcctgg aagccgccag aaatgatcat
     4441 ctggctgaat ttgctggcct tcggtgggtt ggaagccgtt ttcctgtggc cgctggtgct
     4501 gggtctttac tgggaacgcg ccaacgccaa aggcgcgcta agtgcgatga tcgttggcgg
     4561 cgtgctgtat gccgtactcg cgacgctgaa tattcagtac ctgggcttct accctatcgt
     4621 gccctcgtta ctactaagtt tgctggcttt cctggtcgga aaccgtttcg gtacatccgt
     4681 cccgcaagct accgttttga ctactgataa ataaagagtt ttgccatgcc ttggatccaa
     4741 ctgaaactga acaccaccgg cgcgaacgct gaagatctta gcgatgcgct gatggaagcg
     4801 ggtgccgttt ctatcacttt tcaggatacc cacgatacgc cagtatttga gccgctgccg
     4861 ggcgaaacgc gcctgtgggg cgacaccgat gtgattggtc tgttcgacgc tgaaaccgat
     4921 atgaacgacg tggtggcgat tctggaaaac catccgctgc tcggcgcagg cttcgcgcat
     4981 aaaatcgaac aactggaaga taaagactgg gagcgcgaat ggatggataa tttccacccg
     5041 atgcgctttg gtgaacgact gtggatctgc ccaagctggc gtgatgtgcc ggacgaaaac
     5101 gccgtcaacg tgatgttaga cccagggctg gcgtttggta cgggtaccca tccaactacc
     5161 tctctgtgcc tacaatggct cgacagcctc gatttaactg gtaaaacagt catcgacttt
     5221 ggctgcggtt ccggcattct ggctatcgcg gcgctgaaac tgggtgcagc aaaagccatt
     5281 ggtattgata tcgatccgca ggcgattcag gctagccgcg acaacgccga acgtaatggc
     5341 gtttctgacc gtctggaact ctacttaccg aaagatcagc cagaagaaat gaaagccgac
     5401 gtggtggtcg cgaacatcct tgcaggccca ttacgtgaac tggcaccgtt aatcagcgtc
     5461 ctgccggttt caggcggttt gctgggcctt tccggtattc tggcaagcca ggcagagagc
     5521 gtttgtgaag cttatgccga tagcttcgca ctggacccgg tcgtggaaaa agaagagtgg
     5581 tgccgtatta ccggtcgtaa gaattaacct tcgcatcgcc gtagggtgac gcggggcgaa
     5641 gtgcgagcaa gctcacaaaa ggcacgtaaa tttgccggtt atttacgcaa atttgcgtgc
     5701 caaaattttc attcataaag aaaaattgag aacttactca aatttctttg aatgtaaatt
     5761 ttagtcacta ttttctaata tgatgatttt tatgagtaat tatcgcacca cgctcatttt
     5821 aaatgcaatt ctttgatcca tctcagagga ttggtcaaag tttggccttt catctcgtgc
     5881 aaaaaatgcg taatatacgc cgccttgcag tcacagtatg gtcatttctt aactcatgcg
     5941 catcggacaa tatcagctca gaaatcgcct gatcgcagcg cccatggctg gcattacaga
     6001 cagacctttt cggacgttgt gctacgagat gggagccgga ttgacagtat ccgagatgat
     6061 gtcttctaac ccacaggttt gggaaagcga caaatctcgt ttacggatgg tgcacattga
     6121 tgaacccggt attcgcaccg tgcaaattgc tggtagcgat ccgaaagaaa tggcagatgc
     6181 agcacgtatt aacgtggaaa gcggtgccca gattattgat atcaatatgg gttgcccggc
     6241 taaaaaagtg aatcgcaagc tcgcaggttc agccctcttg cagtacccgg atgtcgttaa
     6301 atcgatcctt accgaggtcg tcaatgcagt ggacgttcct gttaccctga agattcgcac
     6361 tggctgggca ccggaacacc gtaactgcga agagattgcc caactggctg aagactgtgg
     6421 cattcaggct ctgaccattc atggccgtac acgcgcctgt ttgttcaatg gagaagctga
     6481 gtacgacagt attcgggcag ttaagcagaa agtttccatt ccggttatcg cgaatggcga
     6541 cattactgac ccgcttaaag ccagagctgt gctcgactat acaggggcgg atgccctgat
     6601 gataggccgc gcagctcagg gaagaccctg gatctttcgg gaaatccagc attatctgga
     6661 cactggggag ttgctgcccc cactgccttt ggcagaggtt aagcgcttgc tttgcgcgca
     6721 cgttcgggaa ctgcatgact tttatggtcc ggcaaaaggg taccgaattg cacgtaaaca
     6781 cgtttcctgg tatctccagg aacacgctcc aaatgaccag tttcggcgca cattcaacgc
     6841 cattgaggat gccagcgaac agctggaggc gttggaggca tacttcgaaa attttgcgta
     6901 aacagaaata aagagctgac agaactatgt tcgaacaacg cgtaaattct gacgtactga
     6961 ccgtttctac cgttaactct caggatcagg taacccaaaa acccctgcgt gactcggtta
     7021 aacaggcact gaagaactat tttgctcaac tgaatggtca ggatgtgaat gacctctatg
     7081 agctggtact ggctgaagta gaacagcccc tgttggacat ggtgatgcaa tacacccgtg
     7141 gtaaccagac ccgtgctgcg ctgatgatgg gcatcaaccg tggtacgctg cgtaaaaaat
     7201 tgaaaaaata cggcatgaac taattcaggt tagctaaatg cttgattaaa aaggcgctac
     7261 tcggcatggg gaagcgcctt ttttataggt gtcacaaagg gagtgaccat gagaacagga
     7321 tgtgaaccga cccggtttgg taatgaagct aagaccatta ttcacggtga tgcgcttgcc
     7381 gaacttaaaa agatccccgc cgaaagtgtc gatctgatct ttgccgaccc accatataac
     7441 atcggtaaaa attttgatgg tctgatcgaa gcctggaaag aagatctgtt tatcgactgg
     7501 ctgtttgaag tgattgcaga gtgccaccgc gttctgaaaa agcagggcag catgtacatc
     7561 atgaacagca cggaaaacat gccctttatc gatctccagt gccgcaagct ttttaccatc
     7621 aaaagtcgca tcgtctggtc atatgacagt tctggagtac aggcaaaaaa acactacggc
     7681 tccatgtacg accccatcct gatgatggtg aaagacgcaa agaactacac attcaacggt
     7741 gatgctattc tggtagaagc caaaaccgga tcgcagcgcg cgttgatcga ttatcgcaaa
     7801 aatcctccac agccatacaa tcatcaaaaa gtaccgggta acgtctggga ttttccgcgc
     7861 gtgcgttatt tgatggatga atatgagaac cacccgacgc aaaaaccgga agccttactg
     7921 aaacgcatta ttctcgcctc ttccaaccca ggcgatatcg ttctcgaccc gtttgccggt
     7981 agctttacta ccggtgccgt agccgtctcc agcgggcgaa aattcattgg tattgagatc
     8041 aacagcgagt acatcaaaat ggggcttcga cggctggatg tcgcgtcgca ttactctgcg
     8101 gaagaactgg cgaaagtgaa aaaaagaaag accggcaacc tgtcaaaacg aagccggtta
     8161 agcgaagttg accccgatct cattgcaaag taaaggaagt gtaagcctgg ttttcagatt
     8221 attcatttcg tgtatatttc ctgccagact tggttaaaca tgcacaggct caggtaatga
     8281 ttcgcaagta ttggtggctc gtcgttttcg ctgtcttcgt ttttctgttt gatactttac
     8341 tgatgcagtg gattgaactg ctgacaacag aaacagacaa atgccgtaat atgaactcag
     8401 ttaatccact aaaactggtt aactgtgacg aactgaattt tcaggacaga atgtgaattt
     8461 acatgacact taattcattc gtttgatgaa ttaatttcgt tatgttttca tctggcatga
     8521 acattcttaa tacgttatcg accagcgcgg gtgcttgttt ataaagatca taacccgcca
     8581 tattcattaa ccagttttga acaattccgc tgaaggcacc attaataata atcatcacaa
     8641 catctaaatc gaggttattt gctacacaac cttgttgctg acacgcctgc aatacttcgc
     8701 ggagagtctg cggattaaag cccatctttt cgcgtatcac tccctcggcc agcatctcat
     8761 cattaaattc acatttgtga tataagattt tcagcaacgc ctgctggcgg ggaattttgg
     8821 caatatattg caagccgaca atcaattttt cacgcaattg ttgaaacggg tcatgctcta
     8881 atccagccgt caagtgttcc tggattaact cccgcaatga aggctgttgc aaccacatct
     8941 cattaaacag ttgagtcttg ttttcgaagt gccagtagat agcgccacgc gtaacgttag
     9001 cggcgtcggc aatgtcgttg agcgtcgtct tgcttacgcc atgctgcgca aactgggcga
     9061 tggcagtttc aatcagttct tgccgggtct tcagagcttc ggctttggtt ctttttgcca
     9121 tgattaatta ttcaggaaat aaatatattc gacacagggt gagaaaatag cgaaggttaa
     9181 tctatcatct aatgtgtatt tatacgagag ggtaacattg agttgctata aatcgtcaaa
     9241 taaacaatgt atattattta cctaaaatac attcgccaca tgaatatata tttcaattta
     9301 cgaggtttta attctgcctc tttcaacccg cgtcaaaata aaacagtaga atattaatct
     9361 ttttttgtgt ttatgtgcct tgagacgcca gtattcataa ctattcctta catcgacgaa
     9421 tgataatttg taggatagca aactgtattt ttctttctgc gagttaacgc gttgcctttc
     9481 tgggtaaata acgcgctttt ggttttttga ggaatagtaa tgacgaaaca tgccaggttt
     9541 ttcctcctgc cctcctttat tctgatctcc gcggctttaa tcgccggttg taacgataag
     9601 ggagaagaga aagctcacgt cggtgaaccg caggttaccg ttcatattgt gaaaacggcc
     9661 ccgttagaag ttaagactga attaccaggc cgcaccaatg cttaccgtat agccgaagtt
     9721 cgcccacagg ttagcgggat cgtactgaat cgtaatttca ctgaaggcag cgatgtacaa
     9781 gcaggccagt ccctgtacca gatcgatccc gcgacctatc aggcaaatta taacagcgcg
     9841 aaaggcgaac tggcgaaaag tgaagccgcc gccgccatcg cgcatttgac ggtaaaacgt
     9901 tacgttccgc tcgtgggtac gaaatacatc agccagcagg agtacgacca ggccattgct
     9961 gatgctcgcc aggccgatgc cgccgtgatt gccgcaaaag gcacagtcga aagcgctcgc
    10021 atcaatctcg cttataccaa agtgactgcg ccaattagcg gacgtatcgg caaatcgacc
    10081 gtgaccgaag gcgctcttgt cactaatggg caaacgactg aactggcgac tgtccagcag
    10141 ctcgatccta tctacgttga tgtgacccaa tccagcaacg attttatgag gctgaagcaa
    10201 tccgtagagc aaggaaattt gcataaggaa aacgccacca gcaacgtaga gttggtcatg
    10261 gaaaacggtc aaacctatcc cctgaaaggt acgctgcaat tctctgatgt gaccgttgat
    10321 gaaagcaccg gctccataac cctacgtgct gtcttcccta acccgcaaca tacgcttttg
    10381 ccgggtatgt atgtgcgtgc acggattgat gaaggcgtcc aacctgacgc cattcttatc
    10441 ccgcaacaag gcgttagccg cacaccgcgt ggtgatgcaa ccgtgctgat tgttaacgat
    10501 aaaagtcagg tggaagcgcg ccctgtcgtt gccagtcagg cgattggcga taaatggttg
    10561 attagcgaag gtctgaaatc tggcgatcaa gtcattgtca gcggcctgca aaaagcgcgt
    10621 ccgggagagc aggttaaagc cactaccgat acccccgcag atactgcatc gaagtaaggt
    10681 aatctgacat ggcaaacttt tttattcgac gaccgatatt tgcatgggtg ctggccatta
    10741 ttctgatgat ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag tatccaacca
    10801 ttgcaccgcc tgcggtttct gtttcggcaa actatccagg tgctgatgcg cagaccgtgc
    10861 aggatacggt gacgcaggtt atcgaacaga atatgaatgg tatcgataac ctgatgtata
    10921 tgtcctccac caacgattcc gccggtagcg tgacaattac ccttaccttc cagtccggga
    10981 ccgatcctga tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc acgccgttgc
    11041 tgccgcagga ggttcagcag caggggatca gtgttgaaaa gtccagtagc agctatttga
    11101 tggtggcggg ttttgtctct gataacccag gcaccacaca ggacgatatc tcggactatg
    11161 tggcctctaa cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac gtacagcttt
    11221 tcggcgcaca gtatgcgatg cgtatctggc tggacgccga tctgctaaac aaatataaac
    11281 tgacaccggt tgatgtgatt aaccagttga aggtacagaa cgatcagatc gctgccggac
    11341 agttgggcgg aacgccagcg ttaccagggc aacagttgaa tgcctcgatt attgctcaaa
    11401 cgcggttaaa aaatccggaa gaattcggca aagtgaccct gcgcgtaaac agtgacggct
    11461 cggtggtacg cctgaaagat gtcgcacggg ttgaacttgg cggtgaaaac tataacgtta
    11521 tcgctcgtat caacggaaaa ccggcggcgg gcctggggat taagctggca accggcgcga
    11581 atgctctcga taccgcgaaa gccattaagg caaaactggc ggaattacag ccattcttcc
    11641 cgcagggaat gaaggttctc tacccttatg acaccacgcc attcgtccag ctttctattc
    11701 acgaagtggt aaaaacgctg ttcgaagcca ttatgctggt gttcctggtg atgtatctgt
    11761 tcttgcagaa tatgcgagca acgctgatcc ccaccattgc ggtacccgtg gtgttgttag
    11821 ggacgtttgc catcctcgcc gcttttggtt actccatcaa cacactaacg atgttcggga
    11881 tggtgcttgc catcgggctg ctcgtcgatg atgcgatagt ggtggtggag aacgtcgagc
    11941 gcgtgatgat ggaggataag ctcccgccaa aagaagcgac ggaaaaatcg atgtcgcaaa
    12001 ttcagggcgc actggtgggt atcgcgatgg tgctgtcagc ggtatttatt ccgatggcat
    12061 tcttcggcgg ttctactggg gcaatttatc gccagttctc tatcaccatc gtttcggcaa
    12121 tggcactttc tgttctggtg gcattgattc ttacccctgc gttatgtgca acgctgctta
    12181 aacccgtctc tgctgagcat cacgaaaata agggcggttt cttcggctgg tttaatacca
    12241 ccttcgatca tagcgttaac cactacacca acagcgtcgg caaaatcctc ggttccacag
    12301 gacgatattt actgatctat gcgctgattg ttgcaggaat ggtggtgttg tttttacgtc
    12361 ttccgtcttc cttcttacct gaagaggatc agggtgtctt tctgaccatg attcagttac
    12421 ccgctggcgc gacgcaagag cggacgcaaa aagtgttgga tcaagtgacg gattactatc
    12481 tgaagaacga gaaagcgaac gttgaaagtg tctttacggt taacggcttt agcttcagcg
    12541 gccaggcaca aaacgccggt atggccttcg tcagtctgaa accgtgggaa gagcgtaatg
    12601 gtgatgaaaa cagtgcggaa gcggtaatcc atcgtgccaa aatggaattg ggcaagatcc
    12661 gcgacggttt tgtcattcca ttcaatatgc cagccattgt tgaactgggc acggcaacgg
    12721 gtttcgactt tgagttaatt gatcaggctg ggctgggtca cgatgcccta acccaggccc
    12781 gtaaccagtt gcttggtatg gcggcgcaac atcctgccag cttagtcagc gtgcgtccta
    12841 atggcctgga agacaccgcg cagtttaaac tggaagttga ccaggaaaag gcgcaggcat
    12901 taagtgtttc actttctgac atcaatcaga ccatttcaac ggcgttgggt gggacttacg
    12961 ttaacgactt catcgaccgt ggtcgcgtga aaaaggtgta tgttcaggcg gatgccaaat
    13021 tccgtatgct gccagaagat gtcgataaac tttatgtccg cagcgccaac ggcgaaatgg
    13081 tgccattctc ggcctttacc acttcacatt gggtgtatgg ctctccgcga ctggaacgct
    13141 acaacggtct gccgtcaatg gagattcagg gggaagccgc gccaggaacc agttccggcg
    13201 atgccatggc gttgatggaa aaccttgcgt caaaattacc tgcgggcatt ggttatgact
    13261 ggacgggtat gtcgtatcag gaacgcttat cgggaaacca ggctcccgct ctagtagcaa
    13321 tttcctttgt ggttgttttc ctgtgccttg ctgcactcta tgaaagctgg tcaattcctg
    13381 tctcggttat gttggtcgtg ccgttaggga ttgtcggcgt gctgctggcg gcgacactct
    13441 ttaatcaaaa aaatgacgtc tactttatgg tgggcttgct aacgacaatt ggcttgtcgg
    13501 ccaaaaacgc tattttgatt gttgagttcg ctaaagatct catggagaaa gagggtaaag
    13561 gtgttgttga agcgacacta atggcagtac gtatgcgtct gcgtcctatc ctgatgacct
    13621 ctctcgcctt tattctcggc gtattaccgc tagctatcag taacggtgca ggcagtggcg
    13681 cgcagaacgc agtgggtatc ggggtaatgg gcggaatggt ctctgcaacg ttgctggcaa
    13741 tcttcttcct accggtgttc tttgtggtga tccgccgttg ctttaaagga taaatcagaa
    13801 acataaaggc actttcgggt gcctttatta ttttcagtga aacccataaa aattaataag
    13861 atattcttcc actcactctt taaaaacttt ctatagttcc cgctcccttc actattttta
    13921 caattcacat aatttgtcat tattcattcc gcaatattta taacctccgg tgataaaatg
    13981 gcattcagct cgttaataag agagttaact tattaagcgt tagcgtttat tactgaggta
    14041 acaccatgaa aagattaatt cctgtggcat tgctcaccgc attgctggcg ggctgtgctc
    14101 acgattcgcc ctgcgttccg gtttatgacg atcagggttg tctggttcac accaatacct
    14161 gtatgaaagg cacgactcag gataactggg aaacggctgg ggctattgcc ggcggggcag
    14221 cggctgttgc tggcctgacg atggggatca tcgctatgtc gaaataactg aacagggcgc
    14281 ggagtttcgc gctctgttta acgatacagt atctttacat aagataaaat attgctatat
    14341 tctgctggct aattctctgc tgatttagca tgttatatcg cgttatttcg ctgctttcga
    14401 aataacgcat caatttaact ttcacacttc ccaccagaaa tatactcacg ctaacaatca
    14461 ttattctgct tagtaaaaca cgcgtaaata ttttgcgcca aaatgtggcg catgtttcat
    14521 tttcgcacca ttgcggggcg ctgtttttat tatttcctgt ctacactcag cttattgcga
    14581 cgcgaaatcg tgcgcaaaag ctggcactac ttttgcttat aagaggatgg ccacagacag
    14641 gtaaaagacg tttcccaaac gtcctataac gataattttc gcctcacagg aagcattatg
    14701 aaaaagatga tgatagccac actggctgcc gccagcgtgc tgcttgccgt tgcaaatcag
    14761 gcgcatgctg gcgcgacgct tgatgccgtt cagaaaaaag gttttgtgca atgcgggatc
    14821 agtgatggat tacttgggtt ctcttatgcc gatgcagacg gtaagttttc aggtattgat
    14881 gttgatgttg atgttgatgt tgatgttgat gtttgtcgta gtgttgccgc tgctgtattt
    14941 ggtgacgaca cgaaagtgaa atatacccca ctcacagcaa aagaacgctt cacggcttta
    15001 cagtcagggg aagtggattt gctctcccgt aatacgacct ggacttcatc tcgcgacgcc
    15061 gggatgggaa tggcatttac tggcgtcact tattacgacg gcattggctt cctgacgcac
    15121 gataaagcgg ggctaaaaag cgcgaaagaa ctggatggcg ctaccgtctg tattcaggcg
    15181 ggtactgata ccgaactcaa cgttgccgac tatttcaagg caaacaatat gaagtacaca
    15241 ccggtgactt tcgatcgctc tgacgaatca gcgaaggcac tggaatctgg tcgctgcgat
    15301 acgctggcct cggatcagtc gcaactgtat gccctgcgca tcaaattaag caacccagct
    15361 gaatggattg tcttaccgga ggttatctct aaagaaccgc ttgggccggt agttcgtcgt
    15421 ggcgatgatg aatggttctc gattgtacgc tggacgcttt tcgccatgct gaatgctgaa
    15481 gagatgggca tcaattccca gaacgtcgat gaaaaagcgg ctaatccagc aacgcctgat
    15541 atggcacatc tgctgggtaa agaaggcgat tacggcaagg atctgaagtt ggataataaa
    15601 tgggcctata acatcatcaa acaggtgggt aactactcgg aaattttcga gcgtaacgta
    15661 ggttcagaaa gcccgctgaa aattaaacgt gggcaaaata atctctggaa taacggcggt
    15721 attcagtacg cgccgcccgt gcgttaagtc ggtgtgaggt aacgagcgtc gctacggcgg
    15781 cgctctcttc agcgttatgc tttccgaggt ttctatgtct catcgccgct caaccgttaa
    15841 aggctcactc tcctttgcca accctacggt tcgtgcctgg ttattccaga tccttgccgt
    15901 tgttgctgtt gtcggaattg ttggttggtt atttcacaac actgtaacga atctcagtaa
    15961 tcgtggcatt acttcaggtt ttgcctttct ggatcgcggc gctggcttcg gtattgtcca
    16021 gcatttgatc gattaccagc agggcgacac ctacggacgc gtttttattg tcggcttact
    16081 caatacgcta ctggtttctg cattgtgtat tgtgttcgct tctgttttag gcttctttat
    16141 cggtctgacg agactttcgg ataactgact gctacgaaag ctatccacaa tttatattga
    16201 gatcttccgt aatattcccc cgctgctgca aatcttcttc tggtactttg ccgtgttgcg
    16261 caatttgccc ggaccacgcg ccaggctgtg agcgcgtttg atctggcctt tttgagcaat
    16321 cgtgggcttt atattccgtc accgcagctg ggagacggat ttattgcgtt tatcctagct
    16381 gttgttatgg ctatagtcct ttctgttggg ctattccgct ttaataaaac ataccagata
    16441 aagaccggac aactgcgccg cacctggccg ttcgccgcag tgttgatcat cggtttgcct
    16501 ttactggcgc aatggctttt tggcgcagca ctacactggg atgtcccagc cctacgaggc
    16561 tttaacttcc gcggcggaat ggttttaatt cctgaactgg cagccttaac gctggcactt
    16621 tcggtttata cctctgcatt tatcgccgag attatccgcg ctgggatcca ggcagtgcct
    16681 tatggtcaac atgaagcggc tcggtcactg gggttacgct acgccaggtc attattcccc
    16741 aggcactgcg agtgattatt ccgccgttaa ccagccagta tctcaacatc gtcaaaaact
    16801 cctctcttgc cgccgctatt ggctatcccg atatggtttc gctgtttgct ggcaccgtgc
    16861 tgaaccagac ggggcaagcc atcgagacga tagccatgac catgtcggtc tatctgatta
    16921 tcagcctgac tatctcgctg ctgatgaata tctataaccg ccgcatcgcg atcgttgaac
    16981 gctaaggagt catgatgaca aaagtattgc tgtctcagcc ctcgcgccca gcgagccata
    17041 actcaagccg cgcgatggtg tgggtgcgaa aaaatctgtt ctccagctgg agcaatagcc
    17101 tgctgactat tggctgcata tggttgatgt gggaactgat cccaccgttg ctgaactggg
    17161 catttttgca ggctaactgg gttggctcaa cgcgtgccga ctgcacaaaa gccggtgcct
    17221 gttgggtctt catccacgaa cgatttggtc agtttatgta tggactttac ccacacgacc
    17281 aacgctggcg aattaaccta gtattactga ttggccttgt gtcaatcgca ccaatgttct
    17341 ggaaaatact cccgcatcgc ggtcgctata ttgcggtctg ggcggtgatt tacccactga
    17401 ttgtctggtg gctgatgtat ggcggttttt ttggtcttga gcgggttgaa acccggcaat
    17461 ggggcgggct gacgctaact ttaattattg catccgttgg gattgcgggg gcgctaccgt
    17521 gggggatctt actggcgtta ggtcgccgct cccatatgcc gattgtgcgt atcttatcgg
    17581 tcatttttat cgagttctgg cgcggcgtac cgctgattac cgtattgttt atgtcttcgg
    17641 tcatgctgcc gttgtttatg gcagaaggca ccagtatcga caaactgatc cgcgcgctgg
    17701 ttggcgtgat cctgtttcag tcagcatatg ttgcggaagt cgtgcgaggc ggattacagg
    17761 ccctacctaa agggcaatat gaagcggcag agtcgctggc gttggggtac tggaaaactc
    17821 aggggctggt tattctgcca caggcgttga agctggtaat tcctgggctg gtaaatacca
    17881 tcatcgcact cttcaaagat accagcctgg tgatcatcat cggcttgttc gatcttttca
    17941 gtagcgttca gcaggcaacc gttgatcccg cctggttggg tatgtcgacg gaagggtatg
    18001 ttttcgccgc gctgatctac tggatctttt gtttcagcat gtcacgttat agccaacatc
    18061 tggaaaaacg ttttaacacc gggcgtacac cgcattgagg acactatgag ccaaatttta
    18121 ctgcaacctg ctaacgcgat gattacgctg gaaaacgtcc ataaatggta tggacaattc
    18181 catgttttga aaaatataaa tttaaccgtg caaccgggag aacggatcgt tctgtgtggc
    18241 ccttctggtt ccggtaaatc gacaaccatt cgttgtatta atcatctgga agaacatcaa
    18301 cagggacgga ttgtcgtaga tggcatcgaa cttaatgaag atatccgcaa tattgagcgc
    18361 gtcaggcaag aagtgggaat ggtatttcag catttcaatc tcttccctca tctgaccgtt
    18421 ttacagaact gtaccctggc accgatttgg gtacgcaaga tgcctaagaa agaggctgaa
    18481 gctctggcga tgcattacct tgagcgggtg agaattgccg aacatgcgca taagtttccc
    18541 ggacagattt caggtggtca gcagcaacgc gttgccattg cgcgttcgct gtgtatgaag
    18601 ccgaaaatta tgttgtttga tgaaccaacg tcggcctcga tccggagatg gtgaaagagg
    18661 tgctggatac gatgattggg ctggcgcagt cgggtatgac aatgttgtgt gtaacacatg
    18721 agatggggtt tgcacgtacc gtcgctgacc gggtaatttt tatggatcgt ggggagatag
    18781 tggaacaagc tgcacctgat gaatttttta cgcatcctaa atcagagcgt acgagggcat
    18841 ttttatcgca ggtaatccat taattgaatg ttagttcgaa aagcaaaaag gccatccttt
    18901 cggatggcct ttttgcttga tttgatgtct ggcagtttat ggcgggcgtc ctgcccgcca
    18961 ccctccgggc cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcga
    19021 gagagtgttc accgacaaac aacagataaa acaaaaggct cagtcggaag actgggcctt
    19081 ttgttttatt tgatgtctgg cagttcccta ctctcgcatg gg
//
LOCUS       DAJVOY010000070        19076 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVOY010000070 DAJVOY010000000
VERSION     DAJVOY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.070"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OQL90_002131"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OQL90_002131"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1368903.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OQL90_002132"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OQL90_002132"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1368904.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OQL90_002133"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OQL90_002133"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1368905.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OQL90_002134"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OQL90_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1368906.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OQL90_002135"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OQL90_002135"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1368907.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OQL90_002136"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OQL90_002136"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1368908.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="OQL90_002137"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="OQL90_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1368909.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OQL90_002138"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OQL90_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1368910.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OQL90_002139"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OQL90_002139"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1368911.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OQL90_002140"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OQL90_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1368912.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OQL90_002141"
     CDS             complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OQL90_002141"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1368913.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="OQL90_002142"
     CDS             complement(15664..16701)
                     /locus_tag="OQL90_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1368914.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17033..17521
                     /gene="yhhY"
                     /locus_tag="OQL90_002143"
     CDS             17033..17521
                     /gene="yhhY"
                     /locus_tag="OQL90_002143"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1368915.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRSSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17758..18957
                     /locus_tag="OQL90_002144"
     CDS             17758..18957
                     /locus_tag="OQL90_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCS1368916.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLSKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKNVMNASH
                     TIKHGAV"
BASE COUNT         4885 a         5049 c         4641 g         4501 t
ORIGIN      
        1 cctttggccc cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat
       61 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaagcac gagaccacag tacaaaagca cgtcgaatag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gctttcagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg
     5041 atgcagttag acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg
     5701 agagcaacaa cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa
     5761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaattc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccacct tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata
     7741 gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagaatc ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat
     8281 cagcgtattc cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg
     8641 ccaggagccc aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat
     8881 gccgctctct ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacaccg ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcaca cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gaacccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 ggagtagttc agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa
    12361 attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc aacatcggcg ctggtccagg
    12481 caccagaaat gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggggcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgcag
    13261 catagcaaac gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat caccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtaggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatacgac accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat
    14101 ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt
    15061 ccatatcctg atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgtac ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcacg ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg
    16201 cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc
    16321 aaagcgacga ttctgatacg gcgtgacgct cagccctttg cttttcgcca acgcaaacag
    16381 ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag
    16501 cttaacatcg ggatcgttta gtacttcgtc gagatcgctg atgaaatgga tatgggaata
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca
    16861 atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata
    16921 ataatcattc tcattcgcgc ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca
    16981 tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga
    17041 gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca
    17101 gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga
    17161 gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt
    17221 gggccatctc accattgacg tgcaacagcg cccacgcagc agtcatgttg ccgattttgg
    17281 tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat
    17341 gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga
    17401 taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa
    17461 gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta
    17521 agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct
    17581 ttaaggtatt tatttaccca atacatatat tgagatttaa ctcataatta attcctaaag
    17641 tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat
    17701 taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg
    17761 agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt
    17821 gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga aatatttaca
    17881 ttctcactct taaataatat taataatacg gggcttggtt cacagttcca tggtataaca
    17941 ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa caataatgaa
    18001 caattattta tgggatttga cttctatcga ataaatagat ttggtagatt ggaaaagtat
    18061 tattatatac aactaagagg cgctttttta tcggctattc atcaccagat cattgaaaac
    18121 caactggata cagaaacaat aactattagt tatgaattta tcctctgtca acatcttatc
    18181 gcaaataccg agttcagcta tttggcactc cctgaaaatt ataaccgatt gtttttacca
    18241 aattcaaaaa accaaacaaa taatcgtttc aaaacgttaa acagcaaagc tattggcagg
    18301 ctacttgctg ctggtggcgt atacaatggg aacattgaag gattcagaga tactgcggaa
    18361 aaactgggtg gagatgcaat aaaaggctat gatcaaatac taaatgaaaa aacagcgggc
    18421 atagcgatag caacagcatc tattctttta acaaagcgtt ctaatgttga tacatataca
    18481 gaaataaata gttacttagg caaacttaga ggtcaacaaa aacttcttga tggtatagac
    18541 ataatagaaa taatatacat taagagacct tcaaaagact tagctaactt acgaaaggag
    18601 tttaataaaa ctgtaagaaa aaattttctt atcaaacttt caaaaacctc cgaagcatct
    18661 ggaagattca acgccgaaga ccttttaaga atgagaaagg gcaatgttcc tctaaattat
    18721 aatgttcacc ataaactatc tctagatgat ggtggtacta atgatttcga aaatttagta
    18781 ttaatcgaaa acgaaccata tcataaagtt tttactaaca tgcaatcacg aatagctaag
    18841 ggaatattag taggtgaaag caaaatcact ccctgggcca ttccatctgg ctcaatttat
    18901 cctcccatga aaaatgtaat gaacgcatcc cacacaatta aacacggagc agtatgatgg
    18961 ttaaaagggt gaaactggca ggtgtggagc ctcgcagatt tccggcatca tagtgcccaa
    19021 tacgggatgc ataaagctcc ctcagactgg aaactccaca aatgaaatat acaccg
//
LOCUS       DAJVOY010000071        18925 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVOY010000071 DAJVOY010000000
VERSION     DAJVOY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.071"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..731
                     /gene="rihB"
                     /locus_tag="OQL90_002145"
     CDS             <1..731
                     /gene="rihB"
                     /locus_tag="OQL90_002145"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS1368917.1"
                     /translation="MRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMAS
                     DGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPEA
                     ARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFENY
                     GLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTKV
                     GITIDTDWFWGLVEECVRGYIKTH"
     gene            831..2080
                     /locus_tag="OQL90_002146"
                     /pseudo
     CDS             831..2080
                     /locus_tag="OQL90_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(2136..3224)
                     /locus_tag="OQL90_002147"
     CDS             complement(2136..3224)
                     /locus_tag="OQL90_002147"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1368918.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3227..4084)
                     /gene="nfo"
                     /locus_tag="OQL90_002148"
     CDS             complement(3227..4084)
                     /gene="nfo"
                     /locus_tag="OQL90_002148"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1368919.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4158..5207)
                     /locus_tag="OQL90_002149"
     CDS             complement(4158..5207)
                     /locus_tag="OQL90_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1368920.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIIVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5307..6188
                     /locus_tag="OQL90_002150"
     CDS             5307..6188
                     /locus_tag="OQL90_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1368921.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6393..7862
                     /gene="lysP"
                     /locus_tag="OQL90_002151"
     CDS             6393..7862
                     /gene="lysP"
                     /locus_tag="OQL90_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1368922.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8155..10135
                     /gene="cirA"
                     /locus_tag="OQL90_002152"
                     /pseudo
     CDS             8155..10135
                     /gene="cirA"
                     /locus_tag="OQL90_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10167..11003)
                     /gene="yeiG"
                     /locus_tag="OQL90_002153"
     CDS             complement(10167..11003)
                     /gene="yeiG"
                     /locus_tag="OQL90_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS1368923.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11261..11929
                     /gene="folE"
                     /locus_tag="OQL90_002154"
     CDS             11261..11929
                     /gene="folE"
                     /locus_tag="OQL90_002154"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1368924.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11946..13103
                     /locus_tag="OQL90_002155"
     CDS             11946..13103
                     /locus_tag="OQL90_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1368925.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13245..14285
                     /gene="galS"
                     /locus_tag="OQL90_002156"
     CDS             13245..14285
                     /gene="galS"
                     /locus_tag="OQL90_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCS1368926.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14565..15563
                     /gene="mglB"
                     /locus_tag="OQL90_002157"
     CDS             14565..15563
                     /gene="mglB"
                     /locus_tag="OQL90_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1368927.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15624..17144
                     /gene="mglA"
                     /locus_tag="OQL90_002158"
     CDS             15624..17144
                     /gene="mglA"
                     /locus_tag="OQL90_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1368928.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17160..18170
                     /gene="mglC"
                     /locus_tag="OQL90_002159"
     CDS             17160..18170
                     /gene="mglC"
                     /locus_tag="OQL90_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1368929.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18413..>18925)
                     /locus_tag="OQL90_002160"
     CDS             complement(18413..>18925)
                     /locus_tag="OQL90_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1368930.1"
                     /translation="IQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGI
                     MQYGNRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYIVYPRINLDK
                     CVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIELGEVKFK
                     KGEKEHPVTL"
BASE COUNT         4515 a         4657 c         5034 g         4719 t
ORIGIN      
        1 ttatgcgtaa acaaatcgtt gccgataata ttcacggtga aaccggactg gatggtccgg
       61 tattcgagtc gctgacccgc caggcagaaa gtacccacgc ggtgaaatac atcattgata
      121 ccctgatggc aagcgatggc gatatcactc tggtgccggt tggtccgctt tcaaatatcg
      181 cggtggcaat gcgtatgcaa cccgcgatcc tgcccaaaat ccgtgaaatt gtgctaatgg
      241 gcggcgctta cggtacaggc aacttcacgc catctgccga gttcaacatc tttgctgatc
      301 cggaagcagc ccgcgtggtg ttcacctccg gcgttccatt agtgatgatg ggcctcgatc
      361 tcaccaacca gactgtttgc accccggacg tgattgcacg gatggaaagg gcaggcggcc
      421 ctgccggaga gctgttcagc gacatcatga acttcactct caaaacgcag ttcgaaaact
      481 acggcctggc tggcggcccg gtgcacgacg ccacctgcat cggttatctg attaaccctg
      541 atggcattaa aacccaggag atgtacgtcg aagtagacgt caacagtggc ccttgctatg
      601 ggcgtaccgt ctgcgacgag ctgggcgttc ttggcaagcc cgctaatacc aaagtcggca
      661 tcactattga taccgactgg ttctggggct tagtcgaaga gtgcgtgcgc ggctacatca
      721 aaacccatta agtgttgata acaagccggg tcagtatcgc aagatccaag cccggctatt
      781 tctgatgggc agaaagccgt cgcccaatat gacatgaaga gataataact atggatgtca
      841 tgagaagtgt tctgggaatg gtggtattgc tgacgattgc gtttttgctg tcagtaaaca
      901 agaagaagat cagcctgcgt accgttggcg cggcgttagt gttacaggtc gtgattggcg
      961 ggattatgct ttggttaccg gcagggcgtt gggtcgctga aaaagtcgct tttggcgtgc
     1021 ataaagtgat ggcgtacagc gacgcgggta gcgcatttat cttcggttcg ctggtcgggc
     1081 cgaaaatgga cacgctgttt gatggcgcag gatttatctt tggtttcagg gtattaccgg
     1141 caattatctt cgtcactgca ctggtgagta ttctctacta catcggtgtg atggggattt
     1201 taattcgcat tctcggcggt atattccaga aagcattaaa tatcagcaag attgagtcat
     1261 tcgtcgcggt caccaccatt ttcctcgggc aaaacgaaat tccggcgatc gtcaaaccct
     1321 ttatcgatca tctgaatcgc aatgaattat ttacagcgat ttgtagtggc atggcctcga
     1381 ttgctggttc gacaatgatt ggttacgccg ccctgggcgt acctgtggaa tatttgctgg
     1441 cggcatcgtt aatggcgatc ccaggcggga tcttgtttgc tcgcctgtta agcccggcta
     1501 cggaatcttc gcaggtttcc tttaataacc tctctttcac cgaaacaccc caaaaagcat
     1561 tattgaagcc gctgcgacag gggcaatgac cgggctgaaa atcgccgccg gtgtagcgac
     1621 agttgttatg gcatttgtcg ccatcattgc gttaattaat ggtattatcg gcggcgttgg
     1681 cggctggttt ggttttgcac atgcctcgct ggagtccatt ttaggttacc tgttggcccc
     1741 attggcgtgg gtgatggggg ttgactggag tgatgcaaat cttgccggga gtttgattgg
     1801 gcagaagctg gcgatcaatg aatttgtcgc ttatctcaat ttctcgccat atctgcaaac
     1861 gggtggcact ctggatgcta aaaccgtggc gattatttct ttcgcgttgt gcggtttcgc
     1921 taactttggt tctatcgggg tggtggtggg ggcgttttct gcggttgcgc cacaccgtgc
     1981 gccggaaatc gcccaacttg gtttacgcgc gctggcggcg gcgacacttt ctaacctgat
     2041 gagtgctgct attgcaggat tctttattgg tttagcgtag tgcgcaattt atgagccgga
     2101 taagatgcgg tcaagcgtcg aatccggcaa cattgttaca ccattggcac taatgaaagc
     2161 gcgttatcgg cagacagggt agggttgttt aagctgccgc tggcgcgcga aattgccgcg
     2221 caggctaccg caaaacgggc gctgtcgcgg aaactgtatc cttcaagaaa gctatatacc
     2281 aggcccgcca taaaaccatc gccagcacca aaactgtcta ctgtcgtatg cgctggcgcg
     2341 gtcagcaaaa attgttctcc atccttttcg ctgcaataaa ctgactcatc gggcaaataa
     2401 acaaacagtt gctgaacacc ttgctgatgc aatgcattca ctgcggcatt acggtcagca
     2461 tcgctggtga tcgcctgtcc ccataaaatt tccagctccg gtaaagtggg tttcagggtg
     2521 tgaatatgcg ccagccagtg tttgattttg cccgctttga attctgaaac ggtatcgaca
     2581 aacaccggga tttcatcagc gagggtaaag acccattcca gcgcctcggc tgtcaggttg
     2641 caatcagcca gtaccacgcc cgcatgacga agtaaatcgc gcgacccgtt cagtagttgc
     2701 ggtgtcaact gttccagcag atgggtatcg ttaatcgcca gcacggtttg atcgtctcga
     2761 ttggcaattg ccagatacgt cgatgtgctt tgaccatgca aacgaacgca gccggagaca
     2821 ttcacgccgg cacggcgcgt ttcttccagg agcatttcgc cataaaagtc atcgccaatc
     2881 actgaaagca aatggacgtc acggcctaac agcgccagat tgtgggcgat gttgcgtccc
     2941 acgccgcccg ccgagcaatg aattgtaccg ggatgagaag ccgcttgcgg gtaacggata
     3001 tccgcgatcc cgcgaatatc catattgatt gtccccacca ctacgcagta ttcctgctcg
     3061 gtgagaatgt aacctttgcc tttaatccgc cctttgcgca ttaaatccat aatatgcgcc
     3121 gcaacgcgcg aacgactgat ttgcagcatg tccgcaattt cgttctgctg aatcagcggg
     3181 ttacgccgta aaattgcaag gatctccttt tcccggttat tcatcttcag gctaccgctt
     3241 tttcagtttg ttgcgctttc agccaggcga tctcttctgc ccagatatcc gggttgatgg
     3301 tttcaaggat cagcggaatg ccgtcgaaac ggtcgtcctg catgatccag cggaacgcat
     3361 catgaccgat attgccttca ccgaggctat gatggcggtc aacgcggctg ccaaaggtgc
     3421 ttttcgcatc gttaaggtgc atcccgcgca gatacttaaa gccaacaata cgggcaaaat
     3481 ccgcgaacgt tttctcgcat tcggctggag tacgcaaatc atacccggca gcgaaagcat
     3541 ggcaggtatc aatgcagacg ccgacacggg atttatcttc cacgccgtcg ataatcgccg
     3601 cgagatgttc gaatttaaac cctaagttac tgccctgacc ggcggtgttt tcaatcaccg
     3661 ctgttacacc ttgagtttta tccagcgcaa tgttgatgga ttcggcaata cgcgcaaggc
     3721 aatcctcttc tgaaatctgc atcagatggc tgccagggtg gaagttgagc aaagaaagcc
     3781 ccagttgttc gcaacgctgc atttcatcta taaaggcatc gcgcgatttt tccagagctt
     3841 cagtgaccgg atgtccgagg ttaatcagat agctgtcgtg gggaagaatt tgcgccgatg
     3901 tgtagtgata tttttcacag gctgctttga attcatcgat ggtttgcgtc gtgagcggtg
     3961 cggcacgcca ctgacgttgg tttttggtga acaaggcaaa cgcggttgcg tcgatttcgg
     4021 cggcgcgaat tgcggcattt gccagaccgc cagcagcact aacgtgcgct ccaatgtatt
     4081 tcatgcgagg actcctgtta aacccgctgg aggaaaacgg taatgatagc gggttaacag
     4141 gagcaagatg tagtggttta tgcgatgacg ctttgaatca catagttaat cgcaccacca
     4201 ccaacaatca gccaggcaaa cagtaccagt gccatcagca gaggtttcgc cccggctttt
     4261 ttcagcgcgc tgacgtgagt ggtcagaccc agcgccgcca tcgccattgc cagcaggaag
     4321 gtatccagcg ttaccagcat gttcaccacg ctctgcggta acaggtggaa cgagttaaag
     4381 atggcaacga tgatgaacaa gatggcaaac cacggaatag tgattttgct tttctcgccg
     4441 ctgttcgccc cagacagctg tttaacacgc gccgccagca ggatgaggaa cggagccagc
     4501 atcatcacgc gcagcatttt ggaaataact gctgcgtttt ccgcatccgg gctgatggca
     4561 tgacctgccg ccaccacctg cgccacttcg tgcacagtag aaccaatgta gataccgaaa
     4621 gtttccggac taaaccattg agacatcagc ggatatatcg ccgggtagag gaaaatcgcg
     4681 acggtcccga agataacaac ggttgcaaca gccacggtta ctttactggc ttccgctttc
     4741 actaccggct cagtcgccag taccgcggca gcaccacaga tactgctacc ggcaccgatc
     4801 aaccagctgg tgtgcttatc cagaccaaac actttctgcc ccaggaagca agccagcagg
     4861 aaggtactgg acagcgtcaa cacgtcaatg atgatcccac tgataccgac atcggcaatt
     4921 tgcgagaacg tcagacggaa gccataaaga atgataccca gacgtaataa atattgcttg
     4981 gcaaacagca caccaccgtc acagcttttc cagatgtgcg gatagatggt gttgcctaaa
     5041 accatcccca acaagattgc gagggtgagg gcactaaacc cggcacccgc aaccgcggga
     5101 atggaaccac cccacagggc gaccccggtg ataactgcac tcagggctaa ccccggaata
     5161 aaatgccaca gtgtacgatg ttgtttctgt aaggtgatat tcgtcataac cctctcctta
     5221 taaccgggca taaggttacg actaactggt ttaaaaataa aattgattat atatttataa
     5281 ttaatcttta taagtggtaa gcgactatgc acatcaccct ccggcagttg gaagtttttg
     5341 cagaagtatt gaaaagtgga tcaaccaccc aggcgtcggt gatgctggcg ttgtcgcaat
     5401 cagcagtgag cgcagccttg accgacctgg aagggcagct tggcgtgcaa ctgtttgatc
     5461 gcgtggggaa aagactggtt gttaatgaac acgggcggct gctctatccg cgtgcgttgg
     5521 cattgcttga acaggcggtt gaaatcgaac aactgtttcg cgaagacaac ggcgcgattc
     5581 gtatctatgc cagtagtacc atcggtaact acattctgcc tgcagttatc gcccgttatc
     5641 gccatgatta tccgcagttg ccgattgaac ttagcgttgg gaacagccag gacgtgatgc
     5701 aagcggtgct ggatttccgc gttgatattg gctttattga aggaccgtgc cacagcactg
     5761 aaatcatttc tgaaccgtgg ctggaagacg agctggtggt tttcgccgcg ccgacttcgc
     5821 cgttggctcg tggtccggtc accttagaac agctggccgc tgcgccgtgg atcctgcgtg
     5881 aacgcggttc cggcacgcgg gagattgtcg attatctatt gctgtcacat ttaccgaagt
     5941 ttgagatggc gatggaatta ggtaactccg aggcaatcaa acatgcggtg cgtcatgggt
     6001 tgggaattag ttgcctgtcg cgacgggtga ttgaagatca attgcaggca ggcacattaa
     6061 gtgaagttgc ggtccctctg ccgcgcctga tgcgtacgtt gtggcgtata catcatcggc
     6121 aaaaacacct ttccaacgcg ctacggcgct ttctggacta ttgcgatccc gcgaatatac
     6181 cgcgttaagt tgctgtacaa gaacatgctg gtgctgtgtc gatttcgtga cgcagcgccc
     6241 tcagcatgca ttcgccagaa aagagattgg ctgctttact tataatccca gggcgatcat
     6301 gaaggtgtct tataaccgtg tatttctgcc ggaaggattg ccaatcgtct gctacaatcg
     6361 cgcctcattt ttaagatgga tagcattttt gtatggtttc cgaaactaaa accacagaag
     6421 cgccgggctt acgccgtgaa ttaaaggcgc gtcacctgac gatgattgcc attggcggtt
     6481 ccatcggtac aggtcttttt gttgcctctg gcgcaacgat ttctcaggca ggtccgggcg
     6541 gggcattgct ctcgtatatg ctgattggcc tgatggttta cttcctgatg accagtctcg
     6601 gtgaactggc tgcatatatg ccggtttccg gttcgtttgc cacttacggt cagaactatg
     6661 ttgaagaagg ctttggcttc gcgctgggct ggaactactg gtacaactgg gcggtgacta
     6721 tcgccgttga cctggttgca gctcagctgg tcatgagctg gtggttcccg gatacaccgg
     6781 gctggatctg gagtgcgttg ttcctcggcg ttatcttcct gctgaactac atctcagttc
     6841 gtggctttgg tgaagcggaa tactggttct cactgatcaa agtcacgaca gttattgtct
     6901 ttatcatcgt tggcgtgctg atgattatcg gtatcttcaa aggcgcgcag cctgcgggct
     6961 ggagcaactg gacaatcggc gaagcgccgt ttgctggtgg ttttgcggcg atgatcggcg
     7021 tagctatgat tgtcggcttc tctttccagg gaaccgagct gatcggtatt gctgcaggcg
     7081 agtccgaaga tccggcgaaa aacattccac gcgcggtacg tcaggtgttc tggcgaatcc
     7141 tgttgttcta tgtgttcgcg atcctgatta tcagcctgat tattccgtac accgatccga
     7201 gcctgctgcg taacgatgtt aaagacatca gcgttagtcc gttcaccctg gtgttccagc
     7261 acgcgggtct gctctctgcg gcggcggtga tgaacgcagt tattctgacg gcggtgctgt
     7321 cagccggtaa ctccggtatg tatgcgtcta ctcgtatgct gtacaccctg gcgtgtgacg
     7381 gtaaagcgcc gcgcattttc gctaaactgt cgcgtggtgg cgtgccgcgt aatgcgctgt
     7441 atgcgacgac ggtgattgcc ggtctgtgct tcctgacctc catgtttggc aaccagacgg
     7501 tatacctgtg gctgctgaac acctccggga tgacgggttt tatcgcctgg ctggggattg
     7561 ccattagcca ctatcgcttc cgtcgcggtt acgtattgca gggacacgac attaacgatc
     7621 tgccgtaccg ttcaggtttc ttcccactgg ggccgatctt cgcattcatt ctgtgtctga
     7681 ttatcacttt gggccagaac tacgaagcgt tcctgaaaga taccattgac tggggcggcg
     7741 tagcggcaac gtatattggt atcccgctgt tcctgattat ttggttcggc tacaagctga
     7801 ttaaaggaac tcacttcgta cgctacagcg aaatgaagtt cccgcagaac gataagaaat
     7861 aagtttcctc ccttccttgc taagccctct caaccgagag ggctttttca attccatttc
     7921 cctgacaaat catgcggata taaaatttaa catttggatt gataattgtt atcgtttgca
     7981 ttatcgttac gccgcaatca aaaaaggctg acaaatcaga ggctgttccg gctttctggg
     8041 atgatcacct gcatacaaat aagtccaccg cgatgctgcc gtacgcaagg ggacgtgaag
     8101 aaggtgtgag cgataaccca ttttattttc gtagttacct cattgagata tggaatgttt
     8161 aggttgaacc ctttcgtacg ggtcaggctg tgtttgtccg ctatttcttg tgcatggcct
     8221 gtgttagcgg tcgatgatga tggcgaaacg atggttgtca ctgcatcttc cgttgaacaa
     8281 aacctcaaag atgcacccgc cagtatcagc gtcattaccc aggaagacct gcagcgaaaa
     8341 ccggtacaga atctgaagga tgtcctcaaa gaagtgcctg gcgtacaact gacgaacgaa
     8401 ggggataacc gtaagggcgt tagtattcgt ggtctggaca gcagctacac cctgattctc
     8461 gtcgacggta aacgcgttaa ctcccgcaat gccgtcttcc gccacaatga tttcgatctg
     8521 aactggatcc cggtcgattc catcgaacgt attgaagtgg ttcgtggccc gatgtcgtcg
     8581 ctatacggct ccgatgcgct cggcggtgta gtgaatatca tcaccaaaaa aatcggtcag
     8641 aaatggtcgg gtaccgttac cgtcgatacc accattcagg aacatcgcga tcgcggtgat
     8701 acctataacg gtcagttctt taccagtgga ccattaattg atggtgtgct gggaatgaaa
     8761 gcttacggca gcctggcaaa acgtgaaaag gatgacccgc aaaactcaac gaccaccgat
     8821 accggagaaa tgccgcgtat tgaaggattc tccagccgcg acggcaatgt cgaatttgcc
     8881 tggacaccga atcaaaatca cgattttact gccggatacg gtttcgaccg tcaggaactc
     8941 gctggacaaa aaccgcctgg aacgccagaa ctactccgtc agccataatg ggcgttggga
     9001 ttacggcacc agcgaactga aatactacgg tgagaaagtc gagaacaaaa accctggcaa
     9061 cagcagcccg ataacttccg aaagcaatac ggtcgacggc aaatacacgt tgccgctgac
     9121 ggcgattaat cagtttctca cggttggcgg tgaatggcgt cacgacaaac ttagcgatgc
     9181 ggtgaacctg accgggggaa ccagctccaa aacgtctgcc agccagtacg cgctgtttgt
     9241 ggaagatgaa tggcggatct tcgagccgct ggcgctgacg accggcgtgc gtatggacga
     9301 tcacgaaacc tacggtgaac actggagtcc gcgtgcctac ctggtttata acgccaccga
     9361 caccgtaacg gtgaaagggg gctgggcgac ggcatttaaa gcaccttctc tgttgcaact
     9421 tagccctgac tggacgagca attcctgccg tggcgcatgt aagattgtgg gtagcccgga
     9481 tctgaaacca gaaaccagcg aaagttggga gctggggctt tactacatgg gtgaagaagg
     9541 ctggctggaa gggggggaat ccagcgttac cgttttccgt aacgatgtga aagatcgtat
     9601 cagcatcagc cgtacgtctg acgtcaacgc tgcaccgggc taccaaaact ttgttggttt
     9661 tgagacgggc gctaacggac ggcgtatacc ggtatttagc tactacaacg ttaacaaagc
     9721 tcgtattcag ggcgtggaaa ccgaactgaa aattccgttc aacgatgaat ggaaactgtc
     9781 gatcaactac acctacaacg atggtcgtga tgtcagcaac ggcgaaaaca aaccgctatc
     9841 cgatctgccg ttccatactg ctaacggtac gctggactgg aaaccgctgg cgctggaaga
     9901 ctggtcattc tatgtttctg gtcactatac cgggcagaaa cgcgccgaca gcgcgacggc
     9961 taaaacaccg ggcggttata ccatctggaa taccggcgcg gcctggcagg tgactaaaga
    10021 cgtcaaactg cgcgcaggcg tgctgaacct tggcgacaag gatctcagtc gtgacgacta
    10081 cagctataac gaagacggac gtcgttactt tatggcagtg gattatcgct tctgatgaga
    10141 agatgcccgg cgaaccgggc ggactttcac ttcagtaaat actgcgcatg gaagcgcagg
    10201 tgatcctcta taaaagaggc gatgaagtag taactgtgat catatcccgg ctgaatacgc
    10261 agcgtcatcg gccacgcttt ctggcgcgcg gcttctgcca gtaccgcagg ttgcaactgg
    10321 tcggcaagaa actgatcatt atcgccctga tcgataagcg tcgggatcgc atcctgcgcg
    10381 ttactggcat acatcagcgc gcaactgtcc cattccagcc atgcattttt gtcctcacct
    10441 aaatagctgc taaacgcttt gattccccac gggacgctgc acggattcac aattggcgca
    10501 aaggccgaaa cgctggtgta tttacccgga tttttcagcg ccataatcag cgcaccgtga
    10561 ccacccattg agtgaccgct aatggcgcag cggtcgctga cattaaattg cgactgaacc
    10621 agcgccggta attcatcgcg cagataatca tacatccggt aatgcgtcgc ccacggcggt
    10681 tgcgtggcat taagataaaa gcctgcgccc tggcctaaat cgtagccatc gtcgttggca
    10741 accttttcgc cgcgcgggct ggtgtctggc atcaccagta caatccccag ttctgccgcc
    10801 acgcgctggg cacccgcctt ggtggtgaag ttctcgtcat tgcaggttaa tccggaaagc
    10861 cagtacagca ctggtggcgg ggtgtgatca cgagggggtg ggagaaagat actgaacgtc
    10921 atcgggcagt ttaaggtact ggagtcgtgt cgccagcgtt gctgccagcc ttcaaaacag
    10981 cggtgctctt cgagcatttc catgcgtggc tccttgttgt gttgtttgca atttcttcat
    11041 cataccgttt attcaatgtg ctgtgagtaa ctttcacttc cgtatttgca taacgatgtt
    11101 ttaacatctg ctgatgaaag gcagcggcaa ttacaataat tatcgctgtg aatactggat
    11161 tatgtgcgcc gcctcacgca caataatcag gctgtaaatc agcttaataa ctttgccccc
    11221 acgcagggcg gaggcgtcac acctgcagga gaaatcataa atgccatcac tcagtaaaga
    11281 agcggccctg gttcatgaag cgttagttgc gcgaggactg gaaacaccgc tgcgcccgcc
    11341 cgtgcatgaa atggataacg aaacgcgcaa aagccttatt gctggtcata tgaccgaaat
    11401 catgcagctg ctgaatctcg acctggctga tgacagtttg atggaaacgc cgcatcgcat
    11461 cgctaaaatg tatgtcgatg aaattttctc cggtctggat tacgccaact tcccgaaaat
    11521 caccctcatt gaaaacaaaa tgaaggtcga tgaaatggtc accgtgcgcg atatcactct
    11581 gaccagtacc tgtgaacacc attttgttac catcgatggc aaagcgacgg tggcttatat
    11641 cccgaaagat tcggtgatcg gtctgtcaaa aattaaccgt atcgtgcagt tctttgccca
    11701 gcgtccgcag gtgcaggaac gtctgacgca gcaaattctt attgcgctac aaacgctgct
    11761 gggcaccaat aacgtggctg tctccattga tgcggtgcat tactgcgtga aagcgcgtgg
    11821 catccgcgat gcaaccagtg ccacaacaac gacctctctt ggtggattgt tcaaatccag
    11881 tcagaatacg cgccacgagt ttctgcgcgc tgtgcgtcat cacaactgat taaaaggcag
    11941 gaaccatgga gcgcaacgta acgctcgatt ttgttcgcgg cgtcgccatt ctggggatcc
    12001 tgctattaaa catcagcgcc tttgggctac caaaggcggc ttatctcaat cccgcctggt
    12061 acggcgctat tacgccgcag gatgcatgga cctgggcatt tctcgatctc atcggccagg
    12121 tgaaattcct cacgcttttt gcgctgctgt ttggtgcggg cctgcaaatg ttgctgcccc
    12181 gtggcagacg ctggatccag tcgcggttaa cgctgttagt cttgctgggt tttattcacg
    12241 gtttattgtt ctgggacggc gatattctgc tggcttacgg gctggtgggc ttaatctgct
    12301 ggcggctggt gcgcgatgcg ccatcggtaa aaagcctgtt taatacaggc gtcatgcttt
    12361 atctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc cagaccagcc
    12421 gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag cttcacggcg
    12481 gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg gcactgggcg
    12541 cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg atgcgcagcg
    12601 gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt gtgctggtgg
    12661 cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg gactgggcgt
    12721 atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg tttcaggcga
    12781 ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt aagctggtgc
    12841 ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa acgctgattt
    12901 gtaccatgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg gagctgctgg
    12961 cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg ctgcgttact
    13021 tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct gccggaacgg
    13081 caatatctaa aacatcaaga taacgatctg gatcacattc gttaacaaaa cggctgtaac
    13141 cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac aaaatagccg
    13201 ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc accattcgtg
    13261 atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc aataacagca
    13321 cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag ctggattatc
    13381 ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt ggcgtggtgg
    13441 tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat ctagtcgctc
    13501 agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa aaagagcgtc
    13561 acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac tcaaaagcat
    13621 tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg ttaatcaacc
    13681 gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc ggtgcccgaa
    13741 tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt tcttccagtc
    13801 acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg aaagagcagg
    13861 atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg ggcggtgagg
    13921 cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctatattt gcttataacg
    13981 acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg attccgttac
    14041 atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac ccgcaattaa
    14101 cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg gccttgcagg
    14161 gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg ttagtgcgtc
    14221 gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca aatcaggcga
    14281 tgtaaccgct ttcaatctgt gagtgatttc acagaatctt aacaatgtga tagctatgat
    14341 tgcaccgttt taacgttgta acccgtatgt aacagtgaat aatcactttc accgaggtaa
    14401 tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg gaagaacgaa
    14461 ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc cctgccgagc
    14521 atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat aagaaggtgt
    14581 taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac gctgctgata
    14641 ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta gtgcgcaagg
    14701 ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat gattctcaga
    14761 atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg gtgaaggcac
    14821 tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa gcgcgtgggc
    14881 aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg gatagctacg
    14941 acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag ggcgatttga
    15001 ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt cagattcagt
    15061 ccgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc acttacgtga
    15121 ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat accgcaatgt
    15181 gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg aacgccaaca
    15241 aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta gaagcactga
    15301 aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca gaagcgctgg
    15361 cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac aaccaggcga
    15421 aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct gatggcacca
    15481 actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat aaagacaacc
    15541 tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc gcccttattg
    15601 tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc ggggaatact
    15661 tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt gataacgtta
    15721 atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt gcaggaaaat
    15781 cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc attttattcc
    15841 agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt atttcgatgg
    15901 tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg tggctggggc
    15961 gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa accaaagcga
    16021 tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca ttatccgttt
    16081 cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa attgtgatta
    16141 tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc actattattc
    16201 gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg gaagaaatct
    16261 tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc accgaaccgc
    16321 tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct cttaatcagc
    16381 gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt aacctaacgt
    16441 cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg gagatcctcg
    16501 gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta tttggtattc
    16561 gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac cataatgcca
    16621 acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca acgggaattt
    16681 atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac tacaaaaata
    16741 aagttggttt actggataaa tcgcggatga aaagcgatac ccagtgggtg attgattcga
    16801 tgcgggtaaa aacaccgggg catcggacgc aaattggttc gctctcaggt ggtaatcagc
    16861 aaaaggtgat tattgggcgc tggctactaa cgcaaccaga aatattaatg ctcgatgaac
    16921 cgacgcgcgg tattgatgtc ggggcgaagt ttgagatcta tcagttaatt gccgaactgg
    16981 cgaagaaagg caaagggatt attattatct cctctgaaat gcctgagttg ttagggataa
    17041 cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgac acaaaaacaa
    17101 caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag gggcttccca
    17161 tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt atttacgtcg
    17221 ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta agtctgttga
    17281 acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc ggtgtggcag
    17341 ggttaattgt cacccagggg accgaccttt ctgcggggcg tcaagtaggg ctggcagcag
    17401 tggtggcagc gacattattg cagtccatgg ataacgccaa taaagtgttc ccggaaatgg
    17461 cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg gtgatcggtt
    17521 tgatcaacgg tctgattatc gcttatctca acgttacgcc gttcattacc acgctcggca
    17581 cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg gcgtcgccaa
    17641 tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg ctggggagtt
    17701 tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc tgggtgttgt
    17761 ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg gaagcggcaa
    17821 aagtatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg tctggcgtgt
    17881 tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc aacaacctcg
    17941 gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta tcgttcagcg
    18001 gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc gtcatcaact
    18061 atggcctgac gtatatcggc gtaaacccat gctggcagta catcatcaaa ggggcgatta
    18121 ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaaatga tgtgaatgtg
    18181 gtccgcattc ggtcataaac gcacataaca tcatgatctg tgtggttttg taggccggat
    18241 aaggcgttca cgccgcatcc ggcaacagga gccaaatgcc ggatgcaacg ctggcgcgtt
    18301 ttatccggcc tacaataagc tcaacatact gatttgtatg gttttgtagg ccggataagg
    18361 cgttcaagcc gcatccggcg acaggaacca aatgccggat gtgacgcggg ttctacaacg
    18421 ttaccgggtg ttctttctcg cctttcttaa acttcacttc cccgagctca atacaaccca
    18481 ccgggcagac gtgaccacaa agcagacaac ccacacattt ctcggtatta caatgcggtg
    18541 tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac
    18601 agcgtccaca gccaacacat ttgtcaagat tgatacgggg atagacaata taactgcggt
    18661 ctaaatcttc cgccgggaca atattgttat tcgccagacc taccatctcc tgcaacgaat
    18721 caaatccctg atcggcgaga taatgcgata acccgctcgc catatcttct acgatgcgat
    18781 tgccgtactg cataatgccg gtggtgacct gtaacgttgc tgcgccgagc aataaaaact
    18841 ccgccgcatc ctcccaggtt tcaatgccgc cgataccgct gattgggaaa tcgcgcagtt
    18901 ctggatgggt gcgcatttgc tgaat
//
LOCUS       DAJVOY010000072        18921 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVOY010000072 DAJVOY010000000
VERSION     DAJVOY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.072"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..911)
                     /locus_tag="OQL90_002161"
     CDS             complement(<1..911)
                     /locus_tag="OQL90_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS1368931.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQAL"
     gene            1378..2610
                     /gene="mdtM"
                     /locus_tag="OQL90_002162"
     CDS             1378..2610
                     /gene="mdtM"
                     /locus_tag="OQL90_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCS1368932.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2651..3931
                     /locus_tag="OQL90_002163"
     CDS             2651..3931
                     /locus_tag="OQL90_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCS1368933.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            4047..5199
                     /locus_tag="OQL90_002164"
                     /pseudo
     CDS             4047..5199
                     /locus_tag="OQL90_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5209..5977
                     /locus_tag="OQL90_002165"
                     /pseudo
     CDS             5209..5977
                     /locus_tag="OQL90_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5974..6231
                     /locus_tag="OQL90_002166"
     CDS             5974..6231
                     /locus_tag="OQL90_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCS1368934.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            6185..7156
                     /locus_tag="OQL90_002167"
     CDS             6185..7156
                     /locus_tag="OQL90_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCS1368935.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7224..8396
                     /locus_tag="OQL90_002168"
     CDS             7224..8396
                     /locus_tag="OQL90_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1368936.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(8409..8963)
                     /gene="kptA"
                     /locus_tag="OQL90_002169"
     CDS             complement(8409..8963)
                     /gene="kptA"
                     /locus_tag="OQL90_002169"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCS1368937.1"
                     /translation="MAKHNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            9213..9896
                     /locus_tag="OQL90_002170"
     CDS             9213..9896
                     /locus_tag="OQL90_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368938.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9893..10354
                     /locus_tag="OQL90_002171"
     CDS             9893..10354
                     /locus_tag="OQL90_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCS1368939.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10367..11539
                     /gene="iadA"
                     /locus_tag="OQL90_002172"
     CDS             10367..11539
                     /gene="iadA"
                     /locus_tag="OQL90_002172"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1368940.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11604..12515
                     /gene="hypT"
                     /locus_tag="OQL90_002173"
                     /gene_synonym="qseD"
     CDS             11604..12515
                     /gene="hypT"
                     /locus_tag="OQL90_002173"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1368941.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12508..12900)
                     /gene="iraD"
                     /locus_tag="OQL90_002174"
     CDS             complement(12508..12900)
                     /gene="iraD"
                     /locus_tag="OQL90_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCS1368942.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12897..12980)
                     /gene="idlP"
                     /locus_tag="OQL90_002175"
     CDS             complement(12897..12980)
                     /gene="idlP"
                     /locus_tag="OQL90_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCS1368943.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            13572..14402
                     /locus_tag="OQL90_002176"
     CDS             13572..14402
                     /locus_tag="OQL90_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1368944.1"
                     /translation="MSIPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14543..15316)
                     /gene="uxuR"
                     /locus_tag="OQL90_002177"
     CDS             complement(14543..15316)
                     /gene="uxuR"
                     /locus_tag="OQL90_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1368945.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15531..16991)
                     /locus_tag="OQL90_002178"
     CDS             complement(15531..16991)
                     /locus_tag="OQL90_002178"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1368946.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(17072..18256)
                     /gene="uxuA"
                     /locus_tag="OQL90_002179"
     CDS             complement(17072..18256)
                     /gene="uxuA"
                     /locus_tag="OQL90_002179"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1368947.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18596..18800
                     /locus_tag="OQL90_002180"
                     /pseudo
     CDS             18596..18800
                     /locus_tag="OQL90_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4419 a         4812 c         4759 g         4931 t
ORIGIN      
        1 tctaatgcct gcatttgctc tgccgaaagt ccggtatatc gttgtatcca ttcaggatcg
       61 gccccattct gcaacaacaa tcggaggagg ctccgttccc cttctacttt cccttctttc
      121 ctccccctct cccttcccaa cagccatcca tcattatgaa ttcgctctgc aattgtcatt
      181 attcgctccc tgtggtgtgg cattcgcctg gtaagttcgc tgataaacgc cttaaaacgc
      241 gcttcatcgc cagtcagtaa aatgtaattt aacagcgcag ttatctggct gtcattagca
      301 cactctgtaa ccagtaatac taccagttgg tcaataagcc ccatcaaatc gcgctggcga
      361 atatgctttt ggatcaactc caacagggcg actctgcgat gctgcacaat ctcgtcgtct
      421 ggcacgacag tgacatccac cagcgggaac gctgcgctat aaagtttccg tgcggtagtc
      481 ggggcggcaa attcgtccag ccagcacagg gaccaggggt aaggactacg gctaccgtga
      541 taaaacagca tcgggataac cagcggtagc ggccggcgtt tatcatgctc gatatggcgc
      601 tgcatcaccg ccatggaata tcgcatcagg cgaaatgcca tatgaatatc ctcgcggctc
      661 tgatgttcaa tcactacgta aatataacca tcaccttcac gggtctttac cgaccacaga
      721 atatcggagt gtagcgcccg caatttttca tcgacaaagc tggcggattc cagttttaag
      781 ctgtcgagat cgcacagttc acgtaaatct ttgggtaagt ggatctccat aaaatcccgc
      841 gcggtgtcag ggtgagtgag aaaggattta aataatgcgt catgtggcgt gctggtcgtg
      901 aagtttgtca tggcgatccg tcacctgtag ataagatggc ccgactttag cgactaacaa
      961 aaccattctc acccggcaat ttctcgttcc ccgaggtgct ttccaggcgt ctgcaaacga
     1021 tacatttttc cggaaacagg ctcgcgtttt acatttcctg tacccggcag atattcccct
     1081 ttccaccgcg ctcttttgat taaccgcaaa ttttaattac acttaaggtg tatattttct
     1141 atgcaaccca tcaattaaag aggtgtaatg tgctgatgac tatttgtaat cgttatacat
     1201 tctgacctgg agtcagaagg tatttctctg tctgtgtgtt cacaggcagt gtggttgatt
     1261 acatgaaatt cagtacattt gcagtctcgt tgcccttctc gcctgccttt cgtcattacc
     1321 gacggtattg aatttcgttt tccccgttgg ggttctccga acaaggagtt gtttgttatg
     1381 ccacgttttt ttgcccgcca tgccgccacg ctgtttttcc cgatggcgtt gattttgtat
     1441 gactttgccg cgtacctgtc gacggatctg atccagccgg ggatcattaa tgtggtccgt
     1501 gattttaatg ccgatgtcag tctggccccg gcagccgtca gtctctatct cgcgggcggt
     1561 atggcgttac agtggctgct ggggccgctt tccgacagga ttggccgcag gccggtgctg
     1621 attaccgggg cgctaatatt tacccttgcc tgcgctgcga caatgttcac aacgtctatg
     1681 acacagtttc ttatcgcgcg tgcaattcag ggcaccagta tctgttttat tgccaccgtt
     1741 ggttatgtca cggtgcagga ggcgttcggc cagacaaaag ggatcaagtt gatggcgatt
     1801 atcacctcca tcgtactgat tgcgccgatt attggcccgc tttccggcgc agctctgatg
     1861 cactttgtgc actggaaagt cctttttgcc atcattgcag ttatgggttt tatctcattt
     1921 gttggcttac tgctggcgat gccagagacg gtgaagcgcg gcgcggttcc atttagcgcc
     1981 aaaagcgtct ttcgcgattt tcgtaatgtc ttttgcaatc ggctgttcct ctttggcgca
     2041 gcaaccatct ctttaagcta tatcccaatg atgagttggg tggctgtctc gccggtgatc
     2101 ctgatcgacg cgggcagctt aacaacttcg cagttcgcct ggacacaggt tccggtgttc
     2161 ggcgcggtga ttgtcgcgaa tgccatcgtg gcgcgtttcg ttaaagatcc gaccgaaccg
     2221 cgctttatct ggcgtgctgt acccattcaa ctggtcggcc tcgcgctgtt gattatcggc
     2281 aatctgctgt caccgcacgt ctggctgtgg tcggtgctgg gcaccagttt gtatgctttc
     2341 gggattggtt tgatcttccc gaccttattc cgctttacgc ttttttccaa taacttaccg
     2401 aaagggaccg tctccgcatc gctgaatatg gtgatcctga tggtgatgtc tgtctcggtc
     2461 gaaatcggcc gctggctgtg gtttaacggc ggtcgcttgc cgtttcatct gttagccgtc
     2521 gtggcgggcg ttatcgtcgt tttcaccctg gcaggattgc ttaatcgcgt gcgccaacat
     2581 caggctgccg agctggcgga ggagcggtga tttttgcgcg atcctgccgt caggctctat
     2641 tcttaacgtt atgaataaac tcattgaact cagacgcgcc aaaatgttgg cgctctcttt
     2701 actgcttatc gccgctgcta cctttgtcgt tacgctgttt ttgccgccca atttttgggt
     2761 gagcggcgtg aaggcgattg ctgaagcggc gatggtcggc gcgctggcgg actggtttgc
     2821 ggtggtggcg ctgtttcgcc gcgtgccgat tccgatcatt tctcgtcata cggcgattat
     2881 cccgcgtaat aaagaccgga ttggcgaaaa tctcggtcag ttcgtgcagg aaaaatttct
     2941 cgatacccag tcgctggtgg cattgattcg acgtcacgaa ccggcgttgt tgattggcaa
     3001 ctggtttagt cagccggaga acgcccgccg cgttggtcag catctgttgc agatcatgag
     3061 cggttttctt gaactgaccg atgatgcgcg tattcagcgc ctgcttaagc gcgcggtcca
     3121 tcgggcgatt gataaggtcg atctttccgg aaccagtgcg ttgatgctgg agagtatgac
     3181 caaaaacgat cgtcatcagg tgctgctcga tacgctgatc gcacagttga tcgcccttct
     3241 ccagcgcgat aaatcgcgca agtttattgc ccagcaaatt gttcgctggc tggagagcga
     3301 gcatccgctg aaagccaaaa ttttgcctac tgaatggctg ggcgaacata gcgcggagtt
     3361 ggtttctgac gcggtgaatt ctttgcttga tgatatcagc cgcgatcgtg cgcatcagat
     3421 ccgtcatgcg tttgatcgcg ccacttttgc cctgatcgac aagctgaaaa acgatccaga
     3481 aatggcagcg cgagccgatg ccgtaaaaag ttatctgaaa gaagatgaag cttttaaccg
     3541 ctatctcagt gaattgtggg gggatttacg ggaatggctg aaagcggata tcaacagtga
     3601 agattctcgt gtgaaagaac gtatcgcgcg ggcgggtcaa tggtttggcg aaacgttaat
     3661 tgccgatgat gccttgcggg catcgttaaa tggtcacctg gaacaagccg cgcaccgcgt
     3721 cgcgcctgag ttttccgcat tcctgacgcg ccacatcagc gatacagtaa aaagctggga
     3781 tgctcgggat atgtcgcggc aaatcgagtt aaatatcggc aaagatctgc agtttatccg
     3841 tgtcaacggt acgctggttg gcggttgtat tgggctaatt ttatatttgt tgtcgcagct
     3901 cccggccttg ttccccctca gcaattttta gaaatccatg aaaaactaag tggctaaccg
     3961 cgagagagat caaataaacc acactaaaag aatggtaata atgtggcctc ttttcattat
     4021 ctcccgtggt acggggaagg aaaatcatgt cacttatcac cgatctaccc gccattttcg
     4081 atcagttctc tgaagctcgc cagaaaggct ttctcaccgt catggatctc aaggagcgcg
     4141 gcattccgct ggttggcact tactgcacct ttatgccgca agagatcccg atggcagccg
     4201 gtgcggttgt ggtttcgctc tgttctacct ctgatgaaac cattgaagaa gcggagaaag
     4261 atctgccgcg caacctctgc ccgctgatta aaagcagcta cggcttcggc aaaaccgata
     4321 aatgccccta cttctacttt tcggatctgg tggtcggtga aaccacctgc gacggcaaaa
     4381 agaaaatgta tgaatacatg gcggagttta agcccgttca tgtgatgcag ttgccgaaca
     4441 gcgttaagga cgatgcctcg cgtgcgttat ggaaagccga gatgctgcgc ttgcaaaaaa
     4501 cggtagaaga acgttttggg cacgagatta gcgaagatgc tctgcgcgat gccattgcgc
     4561 tgaaaaaccg cgaacgtcgc gcactggcca atttttatca tcttgggcag ttaaatcctc
     4621 cggcgcttag cggcagcgac attctgaaag tggtctacgg cgcaaccttc cggttcgata
     4681 aagaggcgtt gatcaatgaa ctggatgcaa tgaccgcccg cgttcgtcag cagtgggaag
     4741 aaggccagcg actggacccg cgtccacgca ttttaatcac cggctgcccg attggcggcg
     4801 cagcagaaaa agtggtgcgg gcgattgaag agaatggcgg ctgggttgtc ggttatgaaa
     4861 actgcactgg ggcgaaagcg accgagctaa tgcgtggcgg aaacgggcga tgtctacgac
     4921 gcgctggcgg ataaatatct ggcgattggc tgctcctgtg tttcgccgaa cgatcagcgc
     4981 ctgcaaatgc tcagccagat ggtggaagaa tatcaggtcg atggcgtagt tgatgtgatt
     5041 ttgcaggcgt gccataccta cgcggtggaa tcgctggcga ttaaacgtca tgtgcgtcag
     5101 cagcacaaca ttccttatat cgctattgaa acagactact ccacctcaga tgtcgggcag
     5161 ctcagtaccc gtgtcgcggc ctttattgag atgctgtaag gagtggcagt gacatattcg
     5221 attggcattg attctggttc taccgccacc aaagggatct tactggcaga cggcgtgatt
     5281 acgcgccgtt tcctcgttcc aacacccttt cgtccggcaa cagcaattac tgaagcctgg
     5341 gaaactctgc gcgaagggtt agagacaacg ccgtttctga cgctcaccgg ctatggacgg
     5401 caactggtgg attttgccga taaacaagtg acggaaatct cctgtcacgg gctgggcgca
     5461 cggtttcttg cgccagcaac gctgcgcggt aatcgacatc ggtggtcagg acagcaaagt
     5521 gattcagctt gatgatgacg gtaacctgtg cgatttcctg atgaatgaca aatgcgcggc
     5581 gggcaccggg cgtttcctgg aagtgatctc gcgcacgctt ggcaccagcg tcgagcaact
     5641 cgacagcatt accgaaaatg tcacgccgca tgccatcacg agtatgtgca cagtgtttgc
     5701 cgaatcagaa gtgatcagcc tgcgctcagc gggcgtcgcg ccagaagcga ttctcgccgg
     5761 agtgattaac gcgatggcgc ggaggagtgc caatttcatt gctcgtctct cctgtgaagc
     5821 gccgattctg tttactggtg gcgttagtca ttgccagacg tttgcccgga tgctggaatc
     5881 tcacctggga atgccggtaa atacccatcc tgatgcgcaa tttgctggcg caattggcgc
     5941 ggcggtaatt ggtcaacgag tgaggacacg ccgatgaaag agtttttatt tttatttcac
     6001 tccacagtcg gcgtcataca aactcgcaaa gcgttgcagg cagcgggcat gacttttcgc
     6061 gtcagcgata ttccccgtga tttacgcggc ggctgcgggt tatgcatttg gctgacctgt
     6121 ccccccggcg aagaaataca atgggtgatc cctgggctga ctgagtcaat ttattgccag
     6181 caggatggtg tctggcgctg cattgcacat taccgggttt ctccgcgtta aggattcatt
     6241 aaggtcgcta cgtatacttc tcgatatctt attgatttgc cttaaaggga ttaccgtgac
     6301 aaaaagtatt tcgttaagta aacgcatctc cgttattgtg attctttttg ccattgtggc
     6361 agtgtgtact ttcttcgtgc aatcttgcgc ccgtaaaagc aatcatgcag catcatttca
     6421 gaattaccac gccaccattg acggcaaaga aattgcaggc ataacgaata atatctcttc
     6481 gctgacctgg tcggcacaaa gcaatactct ttttagcact atcaacaaac ctgccgccat
     6541 tgttgaaatg accaccaatg gtgattttat ccgcacaatt cctctggatt ttgttaagga
     6601 tctcgagacg atcgaataca ttggcgataa tcagtttgtc attagcgatg agcgcgatta
     6661 cgccatttat gtgatttcac tgaccccaaa ctcggaagta aaaatcctca aaaaaataaa
     6721 aatcccgctg caagactccc caaccaattg cggttttgaa gggttagctt attctcgcca
     6781 ggatcacact ttctggtttt ttaaagagaa aaatccgatt gaggtctaca aagttaacgg
     6841 gctgttaagt agcaatgaat tacacatcag caaagacgag gcactacaac ggcaatttac
     6901 tttagatgat gtgtccggcg cagaatttaa ccagcaaaaa aatacgcttc tggtgctgtc
     6961 tcatgaatct cgcgcactcc aggaagtaac cctggttggc gaggttatcg gtgaaatatc
     7021 actaaccaaa ggtagtcgag gtttgtcgca taacatcaaa caggccgaag ggattgcgat
     7081 ggatgcctct ggtaatattt atatcgttag cgaaccaaat cgtttttatc gctttacccc
     7141 taaatcatcg cattaacagg ctggcatatt tcaccttttt gttattaact tgttaacaac
     7201 aaaaatgaca tacaaggtta aacatgcctt cgtcctcgca tcccgtagaa cgcttttctt
     7261 tcagcaccgc gcttttcggg atgctggttc tgaccctagg tatgggatta ggtcgcttcc
     7321 tttatacgcc tatgttgccc gtcattatgg cggaaggggc gttttcattt agccagctct
     7381 cgtggattgc cagcggcaac tatgcggggt atctggctgg cagtctgcta ttttcgtttg
     7441 gcgcatttca ccagccgtcg cgcctacgcc catttctgtt ggtttctgcc ctggcgagag
     7501 ggttgttgat cctcgcaatg gcatggttgc cgccatttat tctggtgtta ttgattcgct
     7561 tcctggcggg tgtcgccagc gccggtatgc tgatttttgg ttcgacgctg attatgcagc
     7621 acacgcgcca tccttttgtg ctggcagctt tgttttctgg cgttggcatt gcactgggca
     7681 acgaatatgt tctggcaggc ctgcattttg ccttctcctc gcaaacgtta tggcaaggcg
     7741 ccggggcgct ttctggcatg atgctgatcg cacttacgct tttaatgccc tcgaaaaaac
     7801 acgccatcgc accaatgcca ttggcaaaaa cggagcaaca gataatgagc tggtggttac
     7861 tggctattct gtacggcctt gcgggatttg gttatatcat cgtcgcgact tacctgccgc
     7921 tcatggcgaa agatgcaggc tcaccattgt taaccgccca tctctggacg ttagtcggct
     7981 tatcaattgt gccaggctgc tttggctggc tatgggcagc aaaacgttgg ggggctttgc
     8041 cttgcctgac ggcgaatttg ctggtgcagg ctatcagtgt gctgcttact ctcgccagcg
     8101 actctcctct cttgcttatc attagtagta tcggttttgg cggtaccttt atgggcacga
     8161 catctctggt gatgactatc gcccgccaac tgagcgttcc gggaaatctc aatcttttag
     8221 gctttgtgac gctcatttac ggcattggac aaatcctcgg cccggcgctg accagtatgc
     8281 tcggcaatgg aacatcggcg cttgccggcg ccactctctg cggcgcggcg gcgttgttta
     8341 ttgctgcgct tatcagcaca gtacaattat ttaagctgca agtggtcact tcatgaggag
     8401 tgtttccctt accactcaag aaattcgacc gcaaccgttg atgtcagcca gactccgttt
     8461 tccgcttgcc agaaaggaat gcctcgtttc gccatttcct gtgctttgac ggttaagaca
     8521 accggcgatc cgtgtcttgc tcccactttg cgtgccgttg cttcatcagc agagagatgc
     8581 acgtaatgac gatcccctgc aatcagcccc tgttttttta tctcgtcaag gaaacggctt
     8641 gccgtaccgt ggtagagaaa ctgtggcggt gttttttcgg caaaggaaat agcgacctgc
     8701 gaagtcgaat gcccctgaac cgcgcggatg cacctaccat cactggaata actaaaacgc
     8761 tttttatcgc tggttgcgac cacggtatcc agcaatgcac gggttaatcg cttgcccgct
     8821 ttttgggcac aaagtatgag cttatcgata tccgcccatc cttcacggtc cagtacgata
     8881 ccaatcgcct ccggtttgtg tcggaggaca aaacttaaaa acttgctcgt atccgccagc
     8941 tctttttcgt tgtgctttgc catctcgttt tcgtccctgg attttctgtt tttatttacc
     9001 aatgtcattg tcactgggtt tatgaatttt ctgcaactca atcctgcgca gaaccttttc
     9061 cacacagcgt aatgtttccc ctatgcaaaa aacgcatagc cagaaacata cttgcatttt
     9121 ccccataccc acatggctga taaggttttt cagttccagg aattttcctg gctcaagcaa
     9181 cacacaaaca caaaaaaaca atggggattg ttatgacaca acaaggggat gctgtagccg
     9241 gggagctggc gacagaaaaa gtgggcatta aagggtatct ggcttttttt ctcaccataa
     9301 tctttttttc cggtgttttt tccggaacag acagctggtg gcgcgttttt gatttttccg
     9361 ttctgaacgg atcgtttggg caactgcctg gtgcgaacgg agccaccacc tcatttcgcg
     9421 gcgcgggtgg tgcgggggct aaagatggct ttctctttgc gctggagctg gcaccgtcag
     9481 tcattctctc tctggggatt atttcaatta ccgatggtct tggtggatta cgcgccgcgc
     9541 agcagttaat gacacctgtg ctaaaaccgt tgttgggcat tccagggatc tgctcgctgg
     9601 cgctgatcgc taacttgcaa aacactgatg ccgccgccgg tatgaccaaa gagctggcgc
     9661 aggaaggtga aattaccgaa cgcgataaag tgattttcgc cgcatatcaa accagcggta
     9721 gcgccatcat caccaactac ttctcttccg gcgtcgcggt gttcgccttt ctgggcacgt
     9781 ccgtaatcgt acctttagcc gtcatcctgg tgtttaaatt tgtcggagcc aacattttgc
     9841 gcgtctggct taacttcgaa gaacgtcgca acccaacgca aggagcgcaa gcatgacaac
     9901 tcaggtccgc aaaaacgtca tggatatgtt tatcgatggc gcgcgccgtg gctttactat
     9961 cgccacgacc aacctgctgc caaacgtggt gatggcgttt gtcatcattc aggcgctgaa
    10021 aattaccggc ctgctcgact gggtggggca tatctgtgaa cccgtaatgg cgctgtgggg
    10081 attacccggt gaagcggcaa ccgtcctttt agccgcatta atgagtatgg ggggcgcagt
    10141 cggtgttgcc gccagtctgg caaccgctgg cgcattaacc ggtcacgatg tcaccgtttt
    10201 actgcccgcg atgtatttaa tgggcaaccc ggtgcaaaat gtcggtcgct gcctggggac
    10261 agccgaagtg aatgccaaat attacccgca tatcattacg gtctgcgtga ttaacgcatt
    10321 gctgtcgatc tgggttatgc agcttattgt ttaacaagga gttaccatga ttgattatac
    10381 cgcagccggt tttaccctgc tgcagggagc gcatttgtat gcgccggaag atcggggaat
    10441 ttgcgatgtc ctcgtcgcta acggcaaaat tatcgccgtt gccagcaata tcccttctga
    10501 cattgtaccg aactgcacgg ttgtcgatct cagtgggcag atcctctgcc caggttttat
    10561 tgatcaacac gtccatttga ttggcggtgg cggcgaagca ggtcccacga cgcgcacgcc
    10621 ggaagtggcg ctaagtcgcc tgacggaagc gggcgtcacg tcagtggttg gtctgctggg
    10681 caccgactct atctctcgcc acccggaatc cctgctcgcc aagacccgtg cgctcaatga
    10741 agaaggcatc agcgcctgga tgctgaccgg cgcttatcat gtcccttccc gcactattac
    10801 gggttccgtg gaaaaagacg tggcgattat cgatcgtgtg attggcgtga aatgcgccat
    10861 ctctgatcac cgttctgccg caccggacgt ttatcacctg gctaatatgg cggcagaatc
    10921 ccgcgttggc ggtttgctcg gcagtaaacc tggcgtcacc gtgttccaca tgggcgacag
    10981 taaaaaggcg ttacagccta tttatgacct gctggaaaac tgcgatgtgc cgatcagcaa
    11041 gctgctgccg acccacgtta accgcaacgt accgttgttt gagcaggcgc tggagttcgc
    11101 gcgcaaaggc ggcaccatcg atatcaccag cagcattgac gaaccggtcg cccctgccga
    11161 aggtattgcc cgcgccgttc aggcgggtat tccgctggca cgcgtcaccc tcagctccga
    11221 cggcaacggt agccagccgt tcttcgatga cgaagggaat ttaacccata tcggtgttgc
    11281 aggttttgaa acgttgctgg aaaccgtgca ggtgctggtc aaagactatg atttcagtat
    11341 cagcgatgcc ctgcgcccgc tcaccagtag cgtagccggt ttccttaacc tgagcgggaa
    11401 aggcgaaatt ctgcctggca atgacgcaga cttactagtc atgacgccag aattgcgcat
    11461 tgagcaggta tatgctcgcg gcaaactgat ggtcaaagac ggcaaagcct gcgtgaaagg
    11521 aacgtttgaa acggcttaaa cggttgtcag ccgccctaat ggacgtagaa tgcccgcaag
    11581 ggcggctgac agagtaaaac gtaatggatg actgttgtgc ggttttgcat aatattgaaa
    11641 ccaaatggct ttatgatttt ctgaccctgg aaaaatgccg caatttttcc caggcggcag
    11701 tcagtcgtaa cgtctcgcaa ccggcattca gccgccgcat ccgcgcgctg gaacaggcga
    11761 ttggcgttga attgtttaac cgccaggtga cgccgctgca actctcagaa caagggaaaa
    11821 tcttccattc gcagatccgc catctgttgc aacagttaga aagtaacctt gcagagctgc
    11881 gtggcggcag cgattacgcg caacgtaaaa tcaagatagc cgctgcacac tctctttccc
    11941 tcggtctgtt accgtccatt atcagccaga tgccgccgct ctttacctgg gcgattgaag
    12001 ctattgatgt cgatgaagca gtcgataaac tgcgtgaagg gcaaagtgac tgtatttttt
    12061 cctttcacga cgaagatttg ctggaagcac cgtttgacca cattcgctta tttgaatctc
    12121 aattgttccc cgtttgcgcc agtgacgaat acggagaagc aatttttaac ctcgcgcagc
    12181 cacactttcc gttactgaat tacagccgca actcctacat ggggcgattg attaatcgca
    12241 ccctgacgcg ccacagcgag ttaagtttca gcaccttttt tgtctcttcg atgagcgagc
    12301 tattaaagca ggttgccctc gacggctgtg ggattgcctg gctgccggag tatgccatcc
    12361 aacaagaaat tcgcagcggg caactcgttg tgctgaatcg ggacgaactg gtgatcccga
    12421 ttcaggccta cgcataccgt atgaataccc gcatgaatcc agttgccgaa cgcttctggc
    12481 gtgaactgcg cgagctggag attgtgctta gctgacattc tcaagcgtcg cactgcgcca
    12541 accgccatta tcccagacca gatcaaacgt tagcggttca tcgtcgtggt acagcattat
    12601 ctcaaggctg atcgccagag tgtggcagta cgcttctttc aaattaacct gcaacgccgc
    12661 aatccgtggc tcatgaaata acagtgcaga acgcagctga cggcaatacc agctctggag
    12721 atgtttgtcg ctcgggttat gcacttgcca ctcgggcatg cctgaaacaa gtaatgacgg
    12781 taatgccgaa tgaggagaat taaaaagagt gaggagatcg ctgcataccg atttacgcag
    12841 caatgacgtt ttattgcctg aaatttcagg taaaacagcc tgaagtgatt ggcgcatcat
    12901 tttgcgcact cctgacgttt agcaacatat gcggcttgcc ctgaacaccg ggcaccactg
    12961 tattgatgct cattttccat agccattccc ttttgttcat tacatccatg tcagtctgag
    13021 acaggcctga atcctgtcag caaagtataa aaccttactt gcctatgaat atctactgac
    13081 ttttcaggaa aaaagtcatg atttgataaa aatcatcaaa ctcaaaaaaa aaccgctagt
    13141 atattctgaa tattccgaat agttacgtag atgaaaatac ttgtcaccgc cgctacttaa
    13201 ttccggtgga aaattattta tttccgcata atcagccatg tattatttga aacaattagt
    13261 ttgtaaataa tataaattga taacatatca aatacatcaa aaataccatt cataggcatt
    13321 attaatatag acatcaatat agcaacatca aattccaatt agtaataaga tatgcttttt
    13381 aattgcatga aaataatttc aaatgagcgt tttttgatac tttagagtgt ttcctaccct
    13441 ttaatatcac cgtgtagagt tgatatcact acaacagaaa catgcatgtc atttttttac
    13501 ttctttttta ctacagaaaa atttatctat ttttctaaac atcggtgaaa tactcataaa
    13561 aggtaactat tatgtctata ccattaagca atgcccttca gagtcaaatt attacagata
    13621 atcacttcct tcatcatcca aaagtcgaca ataagttaac acgtgaatat gaatatacac
    13681 gcatggatac ggaaaatatc tatcttctgc ctcttactcg tggaaataac cataattacg
    13741 atggaaaaag cgttgtcgaa attagaaaac ttaatattgg caaacagtcc tggccattta
    13801 attatgcgac agatacatgc agagaattca atggtattac aacaacagga cgtatgctct
    13861 atcgaaactt gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg
    13921 cgcatgctgc tacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt
    13981 tcgatcctgt tgaggactat acagttcatg aaataacatt aggccctggt tacaatgttc
    14041 ctggttatgc cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa
    14101 gatggaccaa tgagcagcca agaattgata tctatattga tcagataata actgtctcag
    14161 gggtggcaaa ttcttccgga ttcgcccttg ccgcattact taatgctaat attggaatgg
    14221 gaaatgaccc aataattggt atagaagcct atcctggtac cgcggagatc catgcaaaaa
    14281 tgggatataa cgttatcccc ggtgatgaag atgcgccact caaacgcatg actctgcaac
    14341 cgtcatctct tccagaactg tttgaattaa aaaatggtga atggaattat attggcaaat
    14401 aaccactgag aagaatgagt actaacattc aggttatata gatatttaaa caataccctg
    14461 caatttacaa agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacgttta
    14521 cccttgcgct tatcataatc agtcaggcgt cgactttatc cagcggccat gaatcaaaca
    14581 gatagccgtc aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt
    14641 tttccagatg ctgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg
    14701 caaggatctg tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc
    14761 tgtgcaactg gatccacatt ggattgtttt cccgccactg ccaggactga cggaataact
    14821 ccaccagcat gctgttatgc gttgcttcag caatggcgag atggaactgc atgtcaccgc
    14881 tttcgctgct acccggcgcg ctggaagcca gttcacgctc ttccagttgc aatgcctgac
    14941 gcattttgac gatatcttcg cgggtagcct gcagggcggc aaattccgcg atgttgctct
    15001 caagaagctg tcgcgcctgc aacaattcaa aggggccggc atcattgcag acgttggcat
    15061 ccggactgtc tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc
    15121 gcacttccac cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtccgcg
    15181 tgacatcaag catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtacg
    15241 gcgtctttat gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag
    15301 aggtggcaga tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat
    15361 gcggcgtgaa cgccttatcc ggcctacaac gggcaatttt acggcccccg ttaatctcaa
    15421 actataccat tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc
    15481 aaccaaatat cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca
    15541 gccacacact cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca
    15601 acaaagtcgg cattctgcgg cagatcatcg gcaaaaatac cgctcaggcc cagcagcgct
    15661 ttcacgcggt ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg
    15721 tcgaccacgt caatggcatt accctgctca tccacgccct gggtgtaacg catccagcca
    15781 gccacgccca gcgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccggg
    15841 tccagcagac gctgcggtaa cttctggctg ccgtccatcg caatctgcca ggtacggtga
    15901 cgcagagacg ggttgctgaa acgctcgatc agcagcgtcg cataggcctt caggtccgta
    15961 ccctccggca tagacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg
    16021 cgataatccg ggttagtcat ggtgtcggca atagtttcat agccgccgag gtaaccgagg
    16081 tatgccagga aagagtggct gccgttcagc atacgcagct tcatcatttc gaacggcaca
    16141 acgtctgcaa cgaactgtgc gcccacttta tcccagtccg ggcgaccatt aacgaagtta
    16201 tcttcaatca cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc
    16261 agctggtcag caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc
    16321 atggtgcacg ggaaggtgac gttctcttca atccatgccg ccagctgcgg gtcacgtgcc
    16381 tgagccagcc ccagtaccgc gaccttcgcc acatgaccgt tttcacgcac gttatcgcag
    16441 gacatcaccg taaatgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg
    16501 taaccaatcg cggacttcgg cgcagtcggg ttttctagat cgtgcttgat cagcgggtta
    16561 ttgagatcca gttgaccgct tgccgcatca gcgcagtagc ctttttccgt gaccgttaga
    16621 gagacaatcg ccgtttgcgg acgcgccatc gcgttgagaa taccttcgca gccgtcgatt
    16681 tccggatgca gcgcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct
    16741 ttttccgcta cggtgtacag cagttgctgt tttttcaggt tttcgatcag cacgcggtcg
    16801 ttgcctggca tcaggttaac ttcgcagatg ccccagtcgc tgtcggtgct ttccagcaga
    16861 tgatgggtat acagcgcctg atgtgcgcgg tgaaacgccc cgcaaccgag atgcacaatg
    16921 cgtgattcca gacgagaatg atcccatgac ggacgggcaa ccggcagatt gctgtcaaca
    16981 atagtagtca tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa
    17041 ccggcacggc gcgcagccat gccggtggat attaacggct aaagaaagcg cgctggatcg
    17101 ccagttcgac accgcgaact tcggccaggc ctttcagacg accaattgcg gagtaacctg
    17161 ggttggtttt cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga
    17221 tcaggtcttc tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca
    17281 cttcgtacat atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat
    17341 cttcacgcat ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca
    17401 tatcaaccag atcgttgtca gcacgcacgc cgtaggaacc ggtgcacatg gtgaaaccgt
    17461 tcgccatgct gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc
    17521 gcggcaggcc gaggatcggg cgcggcggat cgtccgggtg aaccgccata cgcacgccaa
    17581 cttcttcagc aactggaata atcgctttca ggaagacggc aaagttttcg cgcagcttcg
    17641 ctttatcgat atctttgtac agctccaggt gcttacggaa ctggtcgagg gtataccctt
    17701 cttccgcgcc cggcagaccg gcaatgatgt tacgcgtcag acgcgctttg tcttcatcgc
    17761 tcatagtggc gaagcgttca gctgcctgag caatttcttc ttcggtgtaa tccgcttccg
    17821 cgcctggacg tttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca
    17881 gagctttgga gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg
    17941 gcatgaagtt gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct
    18001 gatagttagc gatccactgc tcatagttgc cagtgtgggt tttgatatct tcgtgaattg
    18061 gtacgctttc tacgacagac cacaccaggc ctgcgtcttc aacgatcgcc ttgcgtttga
    18121 ggatctcttc tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc
    18181 cagttgcgcc cgcctgacgg acatcagcta aagaaaccag atcgtttggg ccgtaccagc
    18241 gccaggtctg ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgc
    18301 ggaaccctgg agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca
    18361 tcggatgaga tggcgtataa gttctacctt ttggtaagat atgtcaaccc aaatcctgaa
    18421 attggttaac caaatcacaa gaatttcact ctcatggttg ttgctgcaaa ttgcagcaaa
    18481 aggtaatcga tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat
    18541 ttacataaca tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca
    18601 tgtgcttaac attctctggg tagtattcgg cattggtctg atgctggtac tgacaacgac
    18661 cgcaggttca gacacattca ctgcattagc aatgcctctt agattatttt gacctgtcag
    18721 taaaagacac gttgaaaacc tggggtctgc tggaactggt caactccgtg gttgggctga
    18781 ttattagcaa ggtagcgtaa aagaacacca gagcctgcca atggcaggct ctgactgatg
    18841 acaaagtcta aaaatgtgcc cggacagtcc cctcgcccct ccggggagag ggttagggtg
    18901 aggggaacag accagcaaag t
//
LOCUS       DAJVOY010000073        18866 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVOY010000073 DAJVOY010000000
VERSION     DAJVOY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.073"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(151..699)
                     /gene="pgsA"
                     /locus_tag="OQL90_002181"
     CDS             complement(151..699)
                     /gene="pgsA"
                     /locus_tag="OQL90_002181"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1368948.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(756..2588)
                     /gene="uvrC"
                     /locus_tag="OQL90_002182"
     CDS             complement(756..2588)
                     /gene="uvrC"
                     /locus_tag="OQL90_002182"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1368949.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(2585..3241)
                     /gene="uvrY"
                     /locus_tag="OQL90_002183"
     CDS             complement(2585..3241)
                     /gene="uvrY"
                     /locus_tag="OQL90_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1368950.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            3536..3712
                     /locus_tag="OQL90_002184"
     CDS             3536..3712
                     /locus_tag="OQL90_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1368951.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            3699..3923
                     /locus_tag="OQL90_002185"
     CDS             3699..3923
                     /locus_tag="OQL90_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1368952.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(3990..4712)
                     /gene="sdiA"
                     /locus_tag="OQL90_002186"
     CDS             complement(3990..4712)
                     /gene="sdiA"
                     /locus_tag="OQL90_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCS1368953.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(4942..5694)
                     /gene="yecC"
                     /locus_tag="OQL90_002187"
     CDS             complement(4942..5694)
                     /gene="yecC"
                     /locus_tag="OQL90_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1368954.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(5691..6359)
                     /gene="tcyL"
                     /locus_tag="OQL90_002188"
     CDS             complement(5691..6359)
                     /gene="tcyL"
                     /locus_tag="OQL90_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1368955.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(6374..7360)
                     /gene="dcyD"
                     /locus_tag="OQL90_002189"
     CDS             complement(6374..7360)
                     /gene="dcyD"
                     /locus_tag="OQL90_002189"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1368956.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(7465..8265)
                     /gene="tcyJ"
                     /locus_tag="OQL90_002190"
     CDS             complement(7465..8265)
                     /gene="tcyJ"
                     /locus_tag="OQL90_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1368957.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(8353..8904)
                     /gene="fliZ"
                     /locus_tag="OQL90_002191"
     CDS             complement(8353..8904)
                     /gene="fliZ"
                     /locus_tag="OQL90_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS1368958.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(8950..9669)
                     /gene="fliA"
                     /locus_tag="OQL90_002192"
     CDS             complement(8950..9669)
                     /gene="fliA"
                     /locus_tag="OQL90_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS1368959.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(9989..11695)
                     /locus_tag="OQL90_002193"
     CDS             complement(9989..11695)
                     /locus_tag="OQL90_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS1368960.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            11961..13367
                     /gene="fliD"
                     /locus_tag="OQL90_002194"
     CDS             11961..13367
                     /gene="fliD"
                     /locus_tag="OQL90_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1368961.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            13392..13802
                     /gene="fliS"
                     /locus_tag="OQL90_002195"
     CDS             13392..13802
                     /gene="fliS"
                     /locus_tag="OQL90_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1368962.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            13802..14168
                     /gene="fliT"
                     /locus_tag="OQL90_002196"
                     /pseudo
     CDS             13802..14168
                     /gene="fliT"
                     /locus_tag="OQL90_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            14246..15733
                     /gene="amyA"
                     /locus_tag="OQL90_002197"
     CDS             14246..15733
                     /gene="amyA"
                     /locus_tag="OQL90_002197"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1368963.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(15767..16180)
                     /locus_tag="OQL90_002198"
     CDS             complement(15767..16180)
                     /locus_tag="OQL90_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1368964.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            16367..17572
                     /gene="yedE"
                     /locus_tag="OQL90_002199"
     CDS             16367..17572
                     /gene="yedE"
                     /locus_tag="OQL90_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1368965.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            17569..17802
                     /gene="yedF"
                     /locus_tag="OQL90_002200"
     CDS             17569..17802
                     /gene="yedF"
                     /locus_tag="OQL90_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1368966.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            17909..18580
                     /locus_tag="OQL90_002201"
     CDS             17909..18580
                     /locus_tag="OQL90_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCS1368967.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(18620..>18866)
                     /locus_tag="OQL90_002202"
     CDS             complement(18620..>18866)
                     /locus_tag="OQL90_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1368968.1"
                     /translation="VFAVPPACTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVN
                     GARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         4664 a         4884 c         4611 g         4707 t
ORIGIN      
        1 ttcattaagc aatcagttaa tcacttgatt ttattatcgt ctggcaatca gtgccgccgt
       61 tcgatgcgtt gcattctact tacctggcgc gatgagtcaa cgatattttt caccactttt
      121 gatcgtttgc tgaaaattac gccgaaacga tcactgatca agcaaatctg cacgcgcagc
      181 gctcaaatat tgcaacattg accacagagt cagcaccgca gccacaaaga aaagtgcaat
      241 accggcgtac tcaacccaaa tgttcggacg ccacagcagc caggccaacg ccaccatctg
      301 ggcagtggtt ttcactttcc caatccagga gacagccacg ctactgcgtt tacccaactc
      361 cgccatccat tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg ttgccgccgg
      421 tagggtcacc caccagctgt gataatgctc ggttaccagc accatggcga tagccacgag
      481 aactttatct gctacagggt caaggaaagc accaaaccgg gtactctggt tccagcggcg
      541 tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc
      601 aaacggcgac caggtgacag gcagataaaa gaccaataca aagaatggga taaggatgac
      661 acggaacagt gtaagcaacg tagggatatt aaattgcata atgacgggta actatctgtt
      721 gtcagtaaga ttacccctat gttgctacag agacatcaat gtttcaacga ccagaagatc
      781 ttttctgcca gaccttgcga aatacccggc acttttgcaa tttcctcgac gctggcgtta
      841 cgtaaacctt gcaaaccgcc catatatttc aacaacattt gccgacgttt tggcccgacg
      901 ccttcaatgg tttccaggga actggtattt ttgaccttcg cccgtttttt acggtgcccg
      961 ccaatcgcgt gatcatgtga ttcatcgcga atatgctgga taacatgcag cgcgggtgaa
     1021 tctggcggca aactaaatcc ctcatcttcc ggctcaaaga acagcgtttc cagtccagcc
     1081 ttacgatctg ctcctttggc aacgccaagt agcagcggat gatttttatc ccatgagaca
     1141 tccagttcgg cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg
     1201 atcacatccg ggatcttact gtcgtcaatg gctttaccat aacgccgacg cagcacctga
     1261 ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa tgttatagcg ccgatactcc
     1321 gcacgcagcg ggccgttagc atcaaacacc acacaggaag cgacggtttg ttcgcccatg
     1381 gtatggctga tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa cacgctggca
     1441 agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa gtttgctggt taaggccgtc
     1501 gccgcattgg tgcgcgcgag tttcagataa cgcgccctat cgccgcgagg tttggtttga
     1561 acattaatct tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt tttatcgcta
     1621 agattaaaat cgagcaggat ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag
     1681 aactgaccta cgaaggtttc taccacctcg ctcagttccg taccgccagg cactttcggg
     1741 aaatagctgc ggctgccgag cactttgccc tgacgaatga acaatacgtg gacacaagcc
     1801 atgcccgcat cgaacgccac accaataacg tcaaggtcgt cgccggtatt ggaaacaaac
     1861 tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc gaatacgtgc cgcttcttca
     1921 aactccagat tctgactggc agtttccata cggctaatga gttgcgtaag cacctgatca
     1981 tctttgccag acaaaaacag gcgcacatac tcgacctgct gagcgtattc ttcttcactc
     2041 accagtcctt caacgcacgg tcccagacag cgccctatct gatattgcag acacggacgc
     2101 gagcgattgc gataaacact attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc
     2161 gccagagttt cacgtacggc atagccattc gggaacgggc cgaaatattc acctttggca
     2221 tgcttcgcac cacgatgcat cgccagacgc gggtgggtat cgccactcag gaagataaaa
     2281 ggataagatt tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag
     2341 ttgtgttcca gcaacagcgc ttcggtttct gtatgagtaa ccgttacatc aatttgctga
     2401 atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa atagctggaa
     2461 agccgttttt tcaggtcttt cgctttgccg acatagataa ccgtaccacc agcatcgtac
     2521 atgcgataaa cgcctggctg gctggttacg gtttttaaaa acgcttttgc gtcaaactga
     2581 tcactcactg acttgataat gtctccgcat tacacagacc atggcgaatt gccaggtgag
     2641 tcagctcaac atcgccatga atgtttagtt tactgaacat acgatagcgg tagctgttca
     2701 ccgttttcgg actgagattg agctgttctg agatctcatt gaccttctgg cccttggtga
     2761 tcatcagcat aatctgcaat tcacgttcag acaaactggc aaatgggctt tctgtttttt
     2821 ctggttcgat ctggcttaac gccatttgtt gagcgatgtc agaagcaatg taacgctgcc
     2881 ctgaatagac agaacgaatc gcactcacga cttcctgcgg agccgcgcct ttgctgaggt
     2941 agcccgcagc accggcctgc atgactttcg ctggtaaagg gttttctgta tgaacggtaa
     3001 gcatgatgat tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc
     3061 caatgcccgg catactcatg tccattagca ccacgtcaac ggcatttgtc cggcaccact
     3121 taacggcgtc ttcaccgcac gatgcctcac cgacgacttt tatacccttt atatcttcca
     3181 gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga acgttgatca
     3241 aagaaatatc tccagaaata gggataacgc tactgatagt tagtcattcg tatattagcg
     3301 gtttttattg caactttgaa acgttaaaaa tggtgggtat tcgatttttc tctcgttttt
     3361 gtcaattcga ttgtccacag ttatggtcac gcccgtcacc aacggcttac cggatcgttt
     3421 cacagtaagc cctacgatga aatgtgacaa aaattgactt tattcagcaa aaatgaaaat
     3481 cagcccgctg aatatgtaac attaattaac ccgaacaata cgggcaaaaa cattcatgat
     3541 aaaaatattt attggtcatt atattaacgt attttatagc actgccgata tcacgctcaa
     3601 aaaacaacca ctgctatttt tagcaaagct tatggtatac tccgccgcct taacattttt
     3661 caccgcaaat tttcattgca acatgacgag gaaaataaat gagtacgcct gatttttcta
     3721 ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc
     3781 tgatgctgca ggcgatggga caagctgacg cgggccgcgt gatgttaaag atggaaaaac
     3841 agcttgcgct gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa
     3901 ttaaacaagc ctaccgtcag taatataaaa ccggctgaga gcgtgccttt cagccggttt
     3961 ttgcatctgg cacgcagaaa gaaaagcgat caaattaagc cagtagcggc cgcgtaacag
     4021 gccacctggg tcttatttgg tgcattgatt tttttctgca tgtttttctg atggaaattg
     4081 accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt cccttccgcc
     4141 gtccatttca gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt catcactatt
     4201 tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtaactg catttttaat
     4261 tgcagttcat cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa ggacaaaaag
     4321 cccagcgccc gatttggcag cattaaatac tgagtgacac cgcggcgtaa accatgtgcg
     4381 tgcgcggctt cccataatgg ctgtgcttcg ctgaataagt catcattcca cattaaatgg
     4441 ccctgactaa agttttcagg gttcagcaca ggatcaattg cgagaaaatt ttttgcctga
     4501 taataactaa cccacgactc agggtaattg gtgtagaaag ccactttagg tcgcgtgaat
     4561 ggtaccgggt ggcgaacaca taacgaatag taatcgtact ccagctgctg agcctgaagc
     4621 tctatttcat ggtagacctc ttctgcggcc tccatcctct gaaaacgcaa cagcatcgtg
     4681 cgacgccaac tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag
     4741 tatcatttat aatgataatt caaactatca cataaataag atttatatat aatttatatt
     4801 attcaggcaa tgaattactt ttgcaagcca tcgcattctc ttatgttatt aatgagttat
     4861 gctgatttgt taagcagttt tatcacgctt gaaatggcgt cccgccccga caggtgaatc
     4921 gtcggggctg attttttctt attattgcag cagaaacttc tcgaggaact gtcgggtgcg
     4981 aggctgctgg ggatcggcaa ataacgcttt tgctgccccc tgctcgacta tccgccccag
     5041 gtccataaaa atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat
     5101 caccatcgtg cgcttttcct gcgccagctg gcggattgtg ttcaggactt cgcccaccag
     5161 ctctggatcc agcgctgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag
     5221 cgcacgcgca atcgctacac gctgctgttg accgccagac aaacgacgtg gatagctggt
     5281 ttctttaccg gccagcccaa ctttcgccag cagctcgcga gcgcgcgccg tggcctcttc
     5341 tttcggttcg cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg
     5401 cggaaacaaa ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag
     5461 cgatttttgc tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt
     5521 tcccgcttcc ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc
     5581 actcggaccg ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag
     5641 caccgtctga ccttggaatt ttttcaccag gttcttaact tcaatggcac tcatttgggt
     5701 tctctctcct ggcgattaag ttgattctca aaatggttct gcaacgtcga taacacagtc
     5761 gccatgatcc agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc
     5821 gaggtaatca actgcgcctg acggaacagc tccggcacct ggattgtcgc ggccagcgac
     5881 gtatctttta ccagactgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc
     5941 tgtggcaaaa tcgcgcgacg catggtctgc cacggcgtca taccaatact ggcagcggct
     6001 tcccactgac ctttatcaat agaagaaatg gcggcgcgca gcgtttcggc ggcataggcg
     6061 gcagtgttca gcgacagacc aatcattgct gacagaatgg gatctaattc gataccaaac
     6121 tgcggcaggc cgtaatagat cataaacagt tgtgcgatga gcggcgtacc acgaaaaata
     6181 gagatataaa aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg
     6241 agaataaacc cgagtagtaa gccaaaaaac atgccgccga tactgagttg cagggtatac
     6301 ccagcccctt tcaacaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg
     6361 agcgttcccg gagctaaacg tggggatgat aggcgaacag cgcaggcgcg ccgccggtat
     6421 gaataaacag aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagcc
     6481 ccgccatcgc ttttccggta tacacaggat caagcagaat gccttccaga cgcgccagca
     6541 atttcactgc ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat
     6601 cccagagtaa aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta
     6661 ggttaaccac tttcggcaat tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt
     6721 cgctttcagg catcaggtgt tccagcccaa cagccaatcc ggcgtgagtt ccggcactgc
     6781 ccgatgcgac caccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca
     6841 gcgcactctc cacataaccc agcgcgccca gagcattaga accgccaacc ggaatgacat
     6901 acgggcgaaa gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat
     6961 cggtcagtgc gtcgcacatt tcaatctggg tattgaacag attcagcaac aaacgattgc
     7021 cgttggttaa atagttttct gcggttgtgc caataggatt ttccagcagc gccacgcagt
     7081 gcaggccgag tttcgccgca actgcggcag tctgccgcac atggttagac tggatcgcgc
     7141 cggcagtaat cagcgtatcg gcaccttcgc gcagggcatc tgccgcgaga aattccagct
     7201 tacgtaattt attgccgccc attgccatgg gtgtgacgtc atcccgtttg atgaaaattt
     7261 cccgtccaag ataatcagaa aagcgcggca gatattcgag cggcgttggc gcgccgataa
     7321 actccagccg tggaaaacgg gttaaattat gcagtggcat aatagcctcc gatgtgtgtt
     7381 gttgtgattt tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag
     7441 cgcccttttt tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga
     7501 aagggcttgc agagtgccat ctttttgcat ttccgcaatt gcatcgttca ctgctttcag
     7561 caggtcttca tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc
     7621 ggttactgcc agcgtatcgt tggttttctt caccagatcc agcgccgcca gacggtcaac
     7681 gaggatcgca tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata
     7741 ggtacgcaca tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtgcccag
     7801 accgacaccc acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc
     7861 ttttttcacc agcgcctgaa taccagaaat ggtgtacggg gtagagaaat cgtatttttt
     7921 cttgcgctca tcagaaatgg tgacctgatt aatcaccaca tcaatacgtt tagagtccag
     7981 cgacgctagc ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt
     8041 tgccagctgt tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa
     8101 actgaacggc ggataagttc cttccagccc taccagtagc gtaccgcgct ctttgacttt
     8161 attaagcaga ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgccacggc
     8221 catcacaccc atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt
     8281 tgttatgtct gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca
     8341 tcttattctt atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc
     8401 ggtacacgtt tgacgctgat agtgttgata cttccgtaat gcgatgcggt aattgttggt
     8461 gctggtggtg ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc
     8521 gagggaaata ttttgctcat gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc
     8581 acgcaccgta cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat
     8641 atcgaaaaaa tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc
     8701 ccatgactgt tgttccactt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat
     8761 ctcgatttta ttcactattt tgccgtcgcg aactaaggta atgcgatcga gtaatttacg
     8821 gcaatgcgcg caatgggcct ggctgtgttt aaagtcttta agatagcggc ttaatggccg
     8881 tcttttcagg tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat
     8941 ttactgacgt tataacttac ccagtttagt gcttaaccgt ttaatggcct ggctgtgtaa
     9001 ctgactgacc cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc
     9061 ttcctggtaa tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatagcttc
     9121 catcacccgc tggcgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg
     9181 atcatcagta accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa
     9241 aagctggctg ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa
     9301 acgttccgct acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat
     9361 tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg
     9421 aagttcatcc agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc
     9481 ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag
     9541 atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac
     9601 cagcgggaca taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag
     9661 tgaattcacg ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc
     9721 agggttttta atcggacgat tagtgggtga aatgaggggt tatttggggg ttaccggtaa
     9781 attccatgca gaaaaaaccc cgccggtggc ggggaagcac gttgctgaca aattgcgctt
     9841 tatgttgccg gatgcggcgt aaacgcctta tccggcctac aaaaatgtgc aaattcaata
     9901 aattgcaatt caccttgtag gcctgataag tgcagcgcat caggcaattt ggcgttgccg
     9961 tcagtctcag ttaatcaggt tacgacgatt aaccctgcag cagagacaga acctgctgcg
    10021 gtacctggtt ggcttttgcc agcacggagt taccggcctg ctggatgatc tgcgctttcg
    10081 acatgttgga cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gcttcagaca
    10141 ggttggtagt ggtgttgttc aggttggtga ctgcagaatc cagacggttc tgaatagcac
    10201 ccagggatga acggaatttg tcgatggagc tgatagcttc atccagggca gccagaggat
    10261 ctttggttgc agtgcctgca ctggtggttt cagtggtcag tttgcctgta gagttaacat
    10321 aagcctgtgc cccaacagta gctgcaaatt taccagttgc acctgcgcca tttgaagcta
    10381 cagttactgt accggtatcc ttatcgacgt tgtagttgat agtgtaagat gcggttgtgg
    10441 tgagctcttt gtctgcatca gtataggtgt cattcgaatt accagcacca ttagtattcg
    10501 caccaccgaa ggacgccgtt gcagactgaa tacctgtatt atacttaatt gtcgcaccag
    10561 taactgctgt gttagccgtt gcagcactct gcactgtgct cagtacagta tcagcgctta
    10621 cagttttggt gtaggttacg ccagaagttg ccgctgcacc aaagttataa gttgtgccag
    10681 acaactggat agaaccagtt gtcgctaagg tactaccgct agcaaacaga tcggacaatt
    10741 tcgctgaaga tgcaatgccg gcgttgtttg tagataggtt accagtggcg tccaggtacg
    10801 cggctttacc accgatggtc acatcgccat tagcatctac atcaaacttc acatcaccag
    10861 tactacgagt ataaacacct gatgcagtag tgccagccgc tggtttcaga gtattcgcaa
    10921 agttaacaac atcgccatct gcaactgttg cttgagtggt gaagttccct ttagccgcat
    10981 cataagtata gatcgcagca ctcgagccag tagtagtaac tgtatctccg gttttcaggc
    11041 gagacagtgc atcgctagcg ctgagtgctg tattgtttgt ggtcacagca taaggacctg
    11101 ttcccgttgc accagcagcg gtcagatcgc tgactgtagc agctttgttc gcaatagtgc
    11161 ctttgccatt cacgttaaaa ccattcagcc ccagcgtatc tgagtcaatt ttcttcagat
    11221 caatcgtgat agtctggccg tcattcgcac caacctgaat tttcattgaa ccgtctttcg
    11281 ccagtacgtt cacgccgttg aactgggtct ggccagatac gcggtcaatt tcgtccagac
    11341 gggatttgat ttcgtcctga atggagtcca gatccgaatc ggagttagtc ccggtagaag
    11401 cctgaaccgt cagttcaaga atacgctgta agttgttgtt gatttcggac agcgcgcctt
    11461 cagtggtctg cgcaacggag ataccgtcgt tggcgttacg ggccgcctga gtcaggcctt
    11521 taatattaga agtaaaacgg ttagcaatcg cctgacctgc ggcgtcatcc ttcgcgctgt
    11581 taatacgcaa gccagaagac agacgctcga tagaactcga cagcgcagac tggttcttgt
    11641 tgatattatt ttgagtgatc agcgagaggc tgttggtatt aatgacttgt gccatgattc
    11701 gttatcctat attgcaagtc gttgattacg tattgggttt ccacccgtcg gctcaatcgc
    11761 cgtcaaccct gttatcgtct gtcgtaaaac aacctttaga atttttttca caaacagcca
    11821 ttttttgtta gtcgccgaaa tactcttttc tctgcccctt attcccgcta ttaaaaaaaa
    11881 caattaaacg taaactttgc gcaattcagg ccaataaccc cggtattcgt tttacgtgtc
    11941 gaaagataaa aggaaatcgc atggcaagta tttcatcgct gggagtcggg tcaggtctgg
    12001 atttaagttc aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta acccctattt
    12061 caaatcagca atcgtcgttt accgctaaac ttagcgccta cggtacgctg aaaagcgcgc
    12121 tgacgacttt ccagaccgcc aatactgcat tgtctaaagc cgatcttttt tccgccacca
    12181 gcactaccag cagcaccacc gcgttcagtg ccaccactgc gggtaacgcc atcgccggga
    12241 aatacaccat cagcgtcacc catctggcgc aggcgcaaac cctgaccacg cgcaccacca
    12301 gagatgatac gaaaacggcg atcgccacca gcgacagcaa actcaccatt caacaaggcg
    12361 gcgacaaaga tccgattacc attgatatca gcgcggctaa ctcatcgtta agcgggatcc
    12421 gtgatgctat caacaacgca aaagcgggcg taagtgcgag catcattaac gtgggtaacg
    12481 gtgaatatcg tctgtcagtc acatcaaatg acaccggcct tgataatgcg atgacactct
    12541 cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt gccagcagca
    12601 acggtatgga ggtctcggtc gccgccaaga atgcgcagct gacggtcaac aacgtcgcca
    12661 ttgagaacag cagcaacacc atcagcgacg cgctggaaaa catcaccctg aacctgaacg
    12721 atgtcaccac gggcaaccag acgctaacca tcactcagga cacctccaaa gcgcaaacgg
    12781 cgattaaaga ctgggtgaat gcctataact cgttaataga taccttcagt agcctgacca
    12841 aatacaccgc cgtagatgcg ggagctgata gccagagttc tagcaatggc gcactgctcg
    12901 gcgactccac gctgcggacg attcagacgc agttgaagtc gatgctgagt aataccgtca
    12961 gttcttccag ctataaaacg ctggcgcaga ttggtatcac gaccgatccc agcgatggca
    13021 aactggaact ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc agcggcgtag
    13081 gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac gaccaccatc ggcagcaacc
    13141 tgaccagttg gctttcgaca acgggcatta ttaaagccgc taccgatggc gttagtaaga
    13201 ccctgaataa gttaactaaa gactacaacg ccgccagcga tcgcattgat gcgcaggtcg
    13261 cgcgctacaa aaaacaattt acccaactgg acgttttaat gacctcgtta aacagcacca
    13321 gcagctactt aacgcagcag ttcgaaaaca acagtaattc caagtaatca ttattcatcg
    13381 ggagacaggt catgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa
    13441 gcgccgtaat gagcgccagc cagcagcagc tggtcaccat gctatttgat ggagtgctga
    13501 gcgcactggt tagagcgcgc ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct
    13561 ctttgtcaaa agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaagaga
    13621 gcaaagacga actaacccaa aacttgattg ctctttatag ctatatggtc aggcgcttgc
    13681 tgcaagccaa tttacgcaac gatgtctccg cagtcgaaga agtggaagca ttaatgcgca
    13741 atattgccga tgcctggaaa gagtcgttac tctccccttc tttgattcag gacccagtct
    13801 gatgaaccat gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct
    13861 catgttacgc ctggcaacgg aagaacaatg ggacgaactc atcgccagcg aaatggcgta
    13921 tgtgaatgcg gtgcaggagt atagcacacc tgactgaaga ggttgccccg tccaccacga
    13981 tgcaggagca gctccgcccg atgctgcgcc tgattctcga caacgaaagc aaggtaaagc
    14041 agttattaca gattcgtatg gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa
    14101 aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg caagataact
    14161 tctcgtgaat cttaacgagc taatggttcg cgaataatcc gattacggct acgcttctaa
    14221 tgttcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat gttttcactg
    14281 gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgccg acggttttaa
    14341 tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat cgggcgggta
    14401 ttcggtcggc tacgactcct ataatttatt tgatttaggc gagtttgatc agaaaggcag
    14461 catccctact aaatatggcg ataaagtaca attgctggcc gccattgacg ccctgaaacg
    14521 taatgacatt gcggtgctgt tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa
    14581 agaagctatt cgcgtgcagc gtgtaaatgc tgacgaccgt acgcaaattg atgaagaaat
    14641 cattgagtgc gaaggctgga cgcgttacac cttccccgcc cgtgccgggc aatactcaca
    14701 gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga
    14761 tggcatattt aaaattgtta acgactacac cggcgaaggc tggaacgatc aggttgatga
    14821 tgaattaggt aatttcgatt atctgatggg cgagaatatc gattttcgca atcatgccgt
    14881 gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat gcgacggttt
    14941 tcgtcttgat gcggtcaaac atattccggc gtggttttat aaagagtgga tcgaacacgt
    15001 acaggaagtt gcgccaaagc cgctgtttat tgtggcggag tactggtcgc atgaagtcga
    15061 taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgttcg atgcgccgct
    15121 gcagatgaaa ttccatgaag catcgcgcat ggggcgcgac tacgacatga cgcagatttt
    15181 cacgggtaca ttagtggaag ccgatccttt ccacgccgtg acgctcgttg ccaatcacga
    15241 cacccaaccg ttgcaagccc tcgaagcgcc ggtcgaaccg tggtttaaac cgctggcgta
    15301 tgccttaatt ttgttgcggg aaaatggcgt tccttcggta ttctatccgg acctctacgg
    15361 tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata tgccaataat
    15421 cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt
    15481 atttttcgac catccgaact gcattgcctt tagccgcagt ggcaccgacg aatatcccgg
    15541 ctgcgtggtg gtcatgtcga acggggatga tggcgaaaaa accattcatc tgggagagaa
    15601 ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagcg taataaccga
    15661 cgaacacggc gaagcaacct tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga
    15721 agaggtgatt taaattcatc cccggcggca agccggggag atttcattac ggcagtggcg
    15781 tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga tccacacgct
    15841 ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg tacagctcac
    15901 gttttacggt aacgttagtg agatcatcag acatcaatgc caacttaccc ggcacagcaa
    15961 tcacccgctg ccactgacgg caatccagag tatcaccctc tttagtgaca atcaggctgc
    16021 ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag tagcccgcca
    16081 gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg gcacagcccg
    16141 ccagcaacat gagcgcgcct gcaattgcga gttttttcat cattcgtcct gcatgagaag
    16201 aaaagaggat tgtggcatta aagccctgat gtcgccagcc tttccaggtt cagagagaaa
    16261 tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc atgatgtatg
    16321 attcgcgact cttgtttcaa accttctgag ttcagaggct acattcatgt catggcagca
    16381 attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca tcgcggcggg
    16441 tattctctcc acttactatt ttggcattac tggcaccttt tgggctgtca cgggtgaatt
    16501 tacccgttgg ggcggccagc tcctgcagct gtttggcgtc catgctgaag agtggggtta
    16561 ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggga tgatgatcct
    16621 cggcatgttt ggcggctgct ttgccgcagc gctgtgggcc aacaatgtca aactgcgcat
    16681 gccacgcagc cgtatccgca ttatgcaggc cattattggc ggtattatcg ctggttttgg
    16741 cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggtattc ctcagttctc
    16801 gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg gcgcacgctt
    16861 taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc
    16921 gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt
    16981 cggtatgctg ggctgggcgc tgctcacggc gatgaaccaa ccaaaactgg ggctggcaat
    17041 gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct ttacttcggc
    17101 gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca ttatcggtat
    17161 ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac ccaaaatcat
    17221 gtgggcggga ccaaacgcgg taattggtgg tttactgttt ggttttggca tcgtgctggc
    17281 aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc actactggtg
    17341 ggtcggtctg ggcaatgtga tcggctcgac gattctggcg tattactggg atgatttcgc
    17401 tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacctttg gcccgatggg
    17461 tggcctgctg gtgacatatt tgctgttgtt tactgcgctg atgttgatta tcggctggga
    17521 aaaacgcttc ttccgccgtg cggcaccgca gactgctaag gagatcgcat gaaaaatatc
    17581 gttcctgatt accgccttga tatggtgggt gaaccctgcc cttatccggc agtcgcaacc
    17641 cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg aagtggtgag cgactgtcca
    17701 cagtcgatca ataatattcc actggatgcg cgtaatcacg gctatacggt gcgggatatt
    17761 cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat tcccgctgtg
    17821 gcccacagcg ggaacatcgt tgcctctgac acatagattt tacccgcccc ctctcctatt
    17881 cttttcagta tgatctcagg aggtttctat gtgtggacgc tttgcccaat cccaaacgcg
    17941 tgaagattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct acgatcccga
    18001 acccattggc agatacaacg tcgcgccggg aaccaaagtt cttctgctca gtgaacgtga
    18061 tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt gggataaacc
    18121 gccgctgatt aacgcccgcg tagaaactgc cgcaaccagt cgcatgttta aaccgctctg
    18181 gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa aagaaggcga
    18241 caaaaaacag ccttttttta tctatcgtgc tgatggacaa cctattttta tggccgcgat
    18301 aggtagcaca ccatttgagc atggtgacga agccgaagga tttttaattg tcactgctgc
    18361 agcagaccag ggtctggtag acattcatga ccgccgcccg ctggttctgt cgccagaagc
    18421 tgcacaggaa tggatgcggc aagagattgg cggtaaagaa gcatcagaaa ttgctaccaa
    18481 cggctgtgtg ccagcaaacc agttcacctg gcacccggta tcgcgcgcgg taggtaatgt
    18541 caaaaaccag ggcgcggagt taattcaacc tgtttgttga catcctccac gccctgaagg
    18601 acgaggctcc ctgctacgtt caggctgtcg cctgaatcat ttcggtgggt tcctgcttca
    18661 acgggcggcc tgactgcacc atctctccac aggcaagaac ggcgtgcccc gccgctaaaa
    18721 tgttacgagc gccatttaca tcggcgttcg ctgtatatcc acatacctgg catctgaatt
    18781 tactttgtga caggcggttt tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg
    18841 tgcacgctgg cggaacagca aacacc
//
LOCUS       DAJVOY010000074        18732 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVOY010000074 DAJVOY010000000
VERSION     DAJVOY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.074"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..883
                     /locus_tag="OQL90_002203"
     CDS             <1..883
                     /locus_tag="OQL90_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1368969.1"
                     /translation="IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGV
                     LGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGM
                     MLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYVTDWL
                     VKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCRG
                     LIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGA
                     LAYIFLVRQPINDPRKD"
     gene            complement(932..2587)
                     /gene="tsr"
                     /locus_tag="OQL90_002204"
     CDS             complement(932..2587)
                     /gene="tsr"
                     /locus_tag="OQL90_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1368970.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2706..3152)
                     /gene="hpaR"
                     /locus_tag="OQL90_002205"
     CDS             complement(2706..3152)
                     /gene="hpaR"
                     /locus_tag="OQL90_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1368971.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3424..4713
                     /locus_tag="OQL90_002206"
     CDS             3424..4713
                     /locus_tag="OQL90_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1368972.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4710..6176
                     /gene="hpaE"
                     /locus_tag="OQL90_002207"
     CDS             4710..6176
                     /gene="hpaE"
                     /locus_tag="OQL90_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1368973.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRDHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6178..7029
                     /gene="hpaD"
                     /locus_tag="OQL90_002208"
     CDS             6178..7029
                     /gene="hpaD"
                     /locus_tag="OQL90_002208"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1368974.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7039..7419
                     /locus_tag="OQL90_002209"
     CDS             7039..7419
                     /locus_tag="OQL90_002209"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1368975.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7530..8333
                     /gene="hpaH"
                     /locus_tag="OQL90_002210"
     CDS             7530..8333
                     /gene="hpaH"
                     /locus_tag="OQL90_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1368976.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8344..9132
                     /gene="hpaI"
                     /locus_tag="OQL90_002211"
     CDS             8344..9132
                     /gene="hpaI"
                     /locus_tag="OQL90_002211"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1368977.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9307..10683
                     /gene="hpaX"
                     /locus_tag="OQL90_002212"
     CDS             9307..10683
                     /gene="hpaX"
                     /locus_tag="OQL90_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1368978.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10693..11579
                     /gene="hpaA"
                     /locus_tag="OQL90_002213"
                     /pseudo
     CDS             10693..11579
                     /gene="hpaA"
                     /locus_tag="OQL90_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11830..13392
                     /gene="hpaB"
                     /locus_tag="OQL90_002214"
     CDS             11830..13392
                     /gene="hpaB"
                     /locus_tag="OQL90_002214"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCS1368979.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13410..13922
                     /locus_tag="OQL90_002215"
     CDS             13410..13922
                     /locus_tag="OQL90_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1368980.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14315..16465
                     /gene="btsT"
                     /locus_tag="OQL90_002216"
     CDS             14315..16465
                     /gene="btsT"
                     /locus_tag="OQL90_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1368981.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16596..16799
                     /locus_tag="OQL90_002217"
     CDS             16596..16799
                     /locus_tag="OQL90_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1368982.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16810..17766
                     /gene="yjiA"
                     /locus_tag="OQL90_002218"
     CDS             16810..17766
                     /gene="yjiA"
                     /locus_tag="OQL90_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1368983.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17868..>18732)
                     /locus_tag="OQL90_002219"
     CDS             complement(17868..>18732)
                     /locus_tag="OQL90_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS1368984.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKES"
BASE COUNT         4285 a         5183 c         4991 g         4273 t
ORIGIN      
        1 catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca ttggcgttgc
       61 cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg ggatgtttat
      121 taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct atcgcaaccg
      181 cgagcacgta gaactgactg ccgttgagca agcttatctc aatgcaggta gcgtcaatgc
      241 ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc gtacaatgtg
      301 gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc tggcctggct
      361 tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt tgatgacggc
      421 tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta ccgactggct
      481 ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg ccgggatgtt
      541 ctgttctgcc gcctttacgc tgatagtacc acaagcgaca acatccatga cggcggttct
      601 gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgcc ggggcttgat
      661 ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga actttgccag
      721 cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata ccacccactc
      781 attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggggcac tggcgtacat
      841 cttcctggtt cgtcagccga tcaacgaccc acgtaaagat taatctcctt atgcccgata
      901 acattttgct tatcgggcat tttcatggcg attaaaatgt ttcccagttc tcctcgctat
      961 ctgccacggc cattttacgc ggcgcagctg gcgtcacggt ttttaccaca gccgatgttt
     1021 cacgctgctg ttgctgaatc cggaacactg ccactgcttc ggtcaggcga ctggcctgct
     1081 cttccagcgc ggcggcggca gcggcagact cttccaccag cgcggcgttc tgttgagtta
     1141 cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc tgctcatcag
     1201 aagcagaagc aatttcgccc ataatgtccg tcacgcgggt cacggcgctg acaatctccg
     1261 ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc cccaccgagt
     1321 cttcaatcag gcttttaatt tcacgagccg cctgggcgct gcgctgggcc agattacgca
     1381 cttctcccgc gaccaccgca aaaccgcgcc cttgctcacc cgcacgcgca gcctcaaccg
     1441 ccgcgttcaa agccagaata ttggtctgga aggcaatgcc gtcaattacg ctgataatat
     1501 cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg ttatctacca
     1561 ctttgccgcc gcgttgcgcc gtttcagaag cacttaacgc cagatggctg gcctggcgcg
     1621 cattttcggc gttctgcttc accgttgcgg tcagttgctc catgctggct gccgtctctt
     1681 ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc gttattgccg gtggcgattt
     1741 cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg gtacgcatca
     1801 gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta gagccatcca
     1861 cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca atcaggcgat
     1921 tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg accgccagta
     1981 cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca ttgttatcgc
     2041 tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac tgcttctcga
     2101 aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg cctgcaccta
     2161 acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt ttgatctctg
     2221 ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc gcccagtttt
     2281 tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg gttgaaccgc
     2341 taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg gtgttacgcg
     2401 tctgcaacaa cgcgacccag ctgccattca gcgtggattg ctgctggcga atggtttgta
     2461 aaacagtgaa attttctttg tcattcttta aggcattaaa gaacagaccg cctgatgtca
     2521 gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc ttgatacgtt
     2581 ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcctgga aagcaaaaac
     2641 tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta tcagatacta
     2701 aaaagttatt cattatcgcc cgggatggct tcttgtcggg aattgcccag agcaataaat
     2761 tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc ttcaatctgc
     2821 cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc ctctttggtt
     2881 agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac taaaccgtcg
     2941 cgttccatcc gcgtgagaat cccggtcagg cttgggcgca aaatgcaggc gcgatacgcc
     3001 agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg ccactgttgc
     3061 tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc ttctcgcgcc
     3121 tggagcaacg caatggttag tgagtcgtgc attatctttc ccctgaaaaa ttcaaaatca
     3181 ttaatataga aacagttaat cgtcacgtta ccgggactgc gctgcccctg caattcctga
     3241 ttttatcaaa attgatgata ttttattaac ttactgatat ttaatatttt attatcttta
     3301 aatgtaaata gtttgttaat tagatcacat ttacatcact tacttttgcc aaactacgca
     3361 atcgggaata aaagcaatca ttaacatatt aatgattaag ataatcgaat ccgaggagtg
     3421 gtaatgaaag gcactatctt cgccgtagcg ttgaaccatc gcagccaact tgatgcatgg
     3481 caggaagcgt tccagcaatc cccctacaaa gccccgccta aaactgcggt ctggtttatt
     3541 aaaccgcgca atacggtgat tggttgcggt gaaccgattc cctttccaca gggtgaaaag
     3601 gtactgagcg gtgcgactgt tgcgctgatt gtgggaaaaa cggcgacgaa agtacgtgaa
     3661 gaagatgcgg cagagtacat cgccggatat gcgctggcta acgacgtcag cctgccggaa
     3721 gagagctttt accgcccggc aatcaaagca aaatgtcgtg atggattctg ccccattggc
     3781 gaaaccgtgg ctctcagcaa tgtcgataat ctgaccatct ataccgagat caacgggcgt
     3841 cctgccgatc actggaatac cgccgattta caacgtaacg ccgcgcagtt gctgagcgcc
     3901 ctgagcgaat ttgccacgct gaatccaggc gacgccattc tgctcggcac gccacaggcg
     3961 cgcgtggaaa tacagccagg cgatcgcgtt cgtgttctcg cagaaggttt cccgccgctg
     4021 gaaaatccgg tagtggacga acgtgaagtg accacgcgca agagcttccc aacgcagcca
     4081 cacccgcacg gcacgctgtt tgccctcggc ctgaactacg ccgaccacgc cagcgaactg
     4141 gaatttaagc caccggaaga accgctggta ttcctgaaag cgccaaatac cctcactggc
     4201 gataaccaga cctccgtgcg tccaaacaat attgaataca tgcactatga agcggagctg
     4261 gtggtagtta ttggcaaaca ggcgcgtaac gtcagcgaag ccgatgccat ggattatgtc
     4321 gcgggctaca ccgtgtgtaa cgactacgcc attcgtgact atctggaaaa ctactaccgc
     4381 cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga tgctttcaac catcgtgccg
     4441 aaagaggcga tcccggaccc gcataatctg acccttcgca ccttcgtcaa cggcgagtta
     4501 cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc ccttcctgat cgcctactta
     4561 agtgaattta tgaccctgaa tccgggcgac atgatcgcca ccggcacacc caaaggctta
     4621 tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag aaggcgtggg ccgcctggtg
     4681 aaccgaattg tgagtgagga tacagcgaaa tgaaaaaagt aaatcattgg atcaacggca
     4741 aaaatgttgc aggtaacgac tacttccaga ccaccaatcc ggcaacgggt gaagtgctgg
     4801 cggatgtggc ctctggcggt gaagcggaga tcaatcaggc ggtagcggca gcgaaagagg
     4861 cgttcccgaa atgggccaat ctgccgatga aagagcgtgc gcgcctgatg cgccgtctgg
     4921 gcgatctgat cgaccagaac gtgccagaga tcgccgcgat ggaaaccgcg gacaccggcc
     4981 tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc ttcccacaac tttgaatttt
     5041 tcgcggaagt ctgccagcag atgaacggca agacctatcc ggttgacgac aagatgctca
     5101 actacacgct ggtgcagccg gtgggcgttt gtgcgctggt atcgccgtgg aacgtaccgt
     5161 ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct gggcaatacc gcggtactga
     5221 aaatgtcgga actctccccg ctgaccgctg accgcctggg tgagctggcg ctggaagccg
     5281 gtattccggc aggcgtgctg aacgtggtac agggctacgg cgcaaccgca ggggatgcgc
     5341 tggttcgtga tcatgacgta cgtgccgtgt cgttcaccgg cggtacggcc accgggcgca
     5401 acatcatgaa aaacgccggg ctgaaaaaat actccatgga actgggcggt aaatcgccgg
     5461 tgctgatttt tgaagatgcc gatattgaac gcgcgctgga cgccgccctg ttcaccatct
     5521 tctcgatcaa cggcgaacgc tgcaccgccg gttcacgcat ctttattcag caaagcatct
     5581 acccggaatt cgttaaacgc tttgccgaac gcgccaaccg tctgcgcgtg ggcgatccga
     5641 acgatccgaa tacccaggtt ggcgcgctta tcagccagca gcactgggaa aaagtctccg
     5701 gctatatccg tctcggcatt gaagaaggcg ccaccctgct ggcgggcggc ccggataaac
     5761 cgtctgacct gcctgcacac ctgaaaggcg gcaacttcct gcgcccaacg gtgctggcag
     5821 acgttgataa ccgtatgcga gtcgcccaag aagagatttt cgggccggtc gcctgcctgc
     5881 tgccgtttaa agacgaagct gaaggcttac gcctggcaaa cgacgtggag tacggcctcg
     5941 cgtcgtacat ctggacacag gatgtcagca aagtgttacg cctggcgcgt ggcattgaag
     6001 ctggcatggt gttcgtcaac acccagaacg tgcgtgacct gcgccagcca tttggcggcg
     6061 taaaagcctc cggcaccggg cgtgaaggcg gtgagtacag cttcgaagtg ttcgcggaaa
     6121 tgaagaacgt ctgcatttcc atgggcgacc atccaattcc gaaatgggga gtctgatatg
     6181 ggtaagttag cgttagccgc caagatcacg cacgtaccgt cgatgtatct ctctgaactg
     6241 ccagggaaaa accacggttg ccgccagggc gcgatcgacg ggcataaaga gatcagcaag
     6301 cgttgccggg aaatgggcgt cgataccatt atcgttttcg atacccactg gctggtcaac
     6361 agtgcttatc acatcaactg tgcagaccat tttgaaggcg tctacaccag caacgagctg
     6421 ccgcatttta tccgcgatat gacctacaac tacgagggca acccggagtt ggggcagctt
     6481 attgccgatg aagccttaaa gctcggcgtg cgggcaaaag cgcacaacat tcccagcctg
     6541 aaactggaat acggcacgct ggtgccgatg cgctacatga atgaagacaa gcacttcaaa
     6601 gtggtctcca tttcggcttt ctgcacggtt cacgattttg ccgacagccg caagctgggc
     6661 gaagcgattc tgaaagcgat cgaacagtac gacggcaccg tggcggtcct tgccagcggt
     6721 tcgttatcgc accgctttat tgacgatcag cgtgcagaag aagggatgaa cagctacacc
     6781 cgcgagttcg accgccagat ggacgagcgc gtggtgaagt tgtggcgcga aggccagttc
     6841 aaagagttct gcaatatgct gccggagtac gccgactact gctacggcga aggcaatatg
     6901 cacgacacgg tgatgctgct ggggatgctc ggctgggata aatacgacgg caaggtggag
     6961 tttatcaccg agctattccc aagctctggc accggtcagg ttaacgctgt tttcccgctt
     7021 cccgcgtaag gacgttttat gccgcacttt atcgttgaat gcagtgacaa catccgcgaa
     7081 gaagccgacc tgccggggtt gttcgccaaa gtgaatccga cgctggcagc cacgggtatt
     7141 tttccgctgg cgggtattcg cagccgcgtg cattgggtcg atacctggca gatggccgac
     7201 gggcagcgtg attatgcctt cgtgcatatg acgttgaaaa tcggcgcagg tcgcagcctg
     7261 gaaagccgtc agcaggcggg tgaaatgctg tttgaactga ttaaaacgca cttcgccgcc
     7321 ctgatggaga gccgcctgct ggcgttgtcg tttgagattg aagagctgca tccgacgctg
     7381 aattttaaac aaaacaacgt gcacgcattg tttaagtagc gcgcagattg cccggtggcg
     7441 ctgcgcttac cgggcctaca aaaccccaaa ccgtacaccg taggccggat aaggcgcagc
     7501 cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac accctgatcg
     7561 cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc tcgctggatt
     7621 acccagagat caccatcgaa gacgcttacg cggtgcagcg cgaatgggtt cgcctgaaaa
     7681 tcgccgaagg ccgtacgctg aaaggccaca aaatcggtct gacctcaaaa gcaatgcagg
     7741 ccagctcgca gatcagcgaa ccagattacg gtgccctgct ggatgacatg ttcttccacg
     7801 atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg gagctggctt
     7861 tcgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtc tacaacgcca
     7921 cggactacgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac atcgatccgg
     7981 aaacccagcg cccgcgcaaa gtgttcgaca ccatttctga taacgccgcc aacgccgggg
     8041 tgatcctcgg tggtcgtccg atcaaaccag acgaactgga tctgcgttgg atctccgccc
     8101 tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggcgtg ctgaatcatc
     8161 cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta caactggaag
     8221 ccgggcaaat cattctcggt ggttcgttca cccgcccggt tccggcgcgt aagggcgaca
     8281 ccttccacgt cgattacggc aacatgggtt ccattagctg ccgctttgtt taaggagaga
     8341 acgatggaaa acagctttaa agcggcgctg aaagcaggcc gtccgcagat cggattatgg
     8401 ctggggctga gtagcagcta cagcgcagag ttactggccg gagcaggatt cgactggttg
     8461 ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca gctacaggcg
     8521 attgcgccct accccagcca gccggtagta cgtccgtcgt ggaacgatcc ggtgcaaatc
     8581 aaacaactgc tggacgtcgg cacacaaacc ttgctggtgc cgatggtaca aaacgctgac
     8641 gaagcccgtg aagcggtacg cgccacccgt tatccccccg ccggtattcg cggtgtgggc
     8701 agtgcgctgg cccgcgcctc gcgctggaat cgtattcctg attacctgca aaaagccaac
     8761 gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa cttaccgcag
     8821 attctggacg tggaaggcgt cgacggcgtg tttatcggcc cggcggatct gagcgccgat
     8881 atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga gcaggcgatc
     8941 gtgcagatcc gtgaatcggg caaagcgccg gggatcctga tcgccaatga gcaactggca
     9001 aaacgctatc tggaactggg cgcgctattt gtcgccgtcg gcgttgacac caccctgctc
     9061 gcccgcgccg ctgaagcgct ggcagcacgg tttggtgcgc aggccaccgc cgtgaagccc
     9121 ggcgtgtatt aatgccggat gcgctgcact tatccggcct acactcgcac caaaccgtag
     9181 gccggataag gcgtttacgc cgcatccggc aaaaagccgt accaaatcgc ggaccggata
     9241 aggcgttacg ccgcatccgc cataaacctt gtcgtaccct acaaaaatcc cattagagga
     9301 agaaaaatga gcgacacctc acctgccata ccggagagta tcgatccggc gaatcagcat
     9361 aaagcgctga ctgccggaca acaggcggtt attaagaagc tatttcgccg cctgatcgtc
     9421 tttctgttcg tgctgtttat cttctcgttc cttgatcgca tcaacatcgg ctttgccgga
     9481 ctcacgatgg gacgcgacct cggtctgagc gccaccatgt ttggcctcgc taccaccctg
     9541 ttctatgccg cttatgtcat cttcggcatt cccagcaaca ttatgctgag tattgtcggt
     9601 gcgcggcgct ggatcgccac catcatggtg ctctggggca tcgcctctac tgccaccatg
     9661 tttgccactg gccccaccag cttgtacgta ctgcgtatac tggttggcat taccgaagcc
     9721 ggctttctgc ctggtattct gctgtattta accttctggt ttccggccta tttccgcgcc
     9781 cgtgccaacg ccttgtttat ggtagcaatg ccggtaacga cagcgttggg ctcgatcgtt
     9841 tccggctaca ttttgtcgct ggatggcgtc atggcattaa aaggctggca gtggctgttt
     9901 ttgctggaag gcttcccgtc ggtattactc ggcgtcatgg tgtggttctg gcttgatgac
     9961 tcaccggaca aagctaagtg gctgacgaaa gaagacaaaa aatgcctgca agagatgatg
    10021 gataacgatc gtctgacgct ggttcagcca gagggagcca tcagccacca cgccatgcaa
    10081 caacgcagta tgtggcggga gatcttcact ccggtggtga tgatgtatac cctggcgtat
    10141 ttctgcctga ccaacacact tagtgcgatc agcatctgga caccgcagat cctgcaaagt
    10201 tttaatcagg gcagcagtaa tatcaccatc ggcctgctgg ccgccgtacc gcagatttgt
    10261 accattctcg ggatggtcta ctggagccgt cactcagatc gccgccagga acgaaggcat
    10321 cacaccgccc ttccttattt gttcgctgcc gcaggttggt tactggcttc ggcaactgat
    10381 cacaacatga tccagatgct ggggatcatt atggcttcga ccggatcatt cagcgcaatg
    10441 gcgattttct ggacaacacc ggatcagtcc atcagcctgc gggcacgagc gatcggtatt
    10501 gcggtgatca acgccactgg caacattggt tcagcgttaa gtccgtttat gatcggctgg
    10561 ttgaaagatt tgaccggcag ctttaacagt ggattgtggt ttgttgccgc gctgctggtg
    10621 attggtgcgg ggattatctg ggcgattcca atgcagtcct cccgtccgcg agcgaccccg
    10681 taaggaacga cgatgtgtga ccgtcagatt gccaatattg atatcagcaa agagtacgat
    10741 gaaagcctgg gcacggacga tgtgcattat cagtcgttcg cccgcatggc ggcatttttt
    10801 ggccgccata tgctgccaca tcgccacgaa cagtactttc agatgcattt cctcaatagc
    10861 ggacagattg agctacagct tgacgatcat cgctactcgg tggaagcgcc cctgtttgtc
    10921 ctgacgccgc cgtcagtacc tcatgcgttt attacggagt ctgacgccga cggtcatgtg
    10981 ttgacggtac gggaagatct gatctggccc ctgctggaag ttctttatcc gggcactcgg
    11041 gaaaccttcg gcctgccggg ggcctgtcac tggcagataa acccgacgaa ctggcggcgc
    11101 tggaacacta ttggcaactg atagagcggg aatcggtgga acaactgcct ggacgggaac
    11161 acaccctgac gttactggca caggcagtgt tcaccctact gctgcgtaac gcaaaactcg
    11221 acgaccatgc cgccagcggg atgcgcggag aattaaaact gttccagcgt tttaatatgc
    11281 tgattgaaag ccattttcat cagcactgga cagtaccgga ttacgctaac gaactgcata
    11341 tcaccgaatc acgactcacg gacatctgcc gtcgctttgc caaccgtccg ccaaaacggt
    11401 tgattttcga caggcagctg cgagaagcca agcggctgct gctgttttct gataacgccg
    11461 tgaacaatat tgcctggcaa ctcggtttta aggatccggc ttattttgcg cgctttttta
    11521 atcgcttagt cggttgctcg cccagtgctt atcgcgccaa aaaagtacct gtgacgtgaa
    11581 aatcccttag ccttaacggg aaaccaggca ccacctgcta ttcccctttc tccgcctgga
    11641 gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac ctcgatcaca aaaccgtacc
    11701 cgacctgaaa agtaccagcc attgtcccaa aagtctcttc ccgtaatcgc ccctgcaagc
    11761 tttaattgaa taacaaaaac aacacacaaa attaacaatc gtattccgat taatactgta
    11821 gaggtcgaca tgaaaccaga agatttccgc gccagtaccc aacgtccgtt caccggggaa
    11881 gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga gcgagtgaaa
    11941 gacgtcacta ctcatccggc atttcgtaat gcggcagcgt ctgttgccca actgtacgac
    12001 gcgctacaca aaccggagat gcaggactct ctgtgctgga acaccgacac cggcagcggc
    12061 ggctataccc ataaattctt ccgcgtggcg aaaagtgcca acgacctgcg ccagcaacgc
    12121 gatgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac cccagactac
    12181 aaagctgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca gttcgagcag
    12241 aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa ccacgcgatt
    12301 gttaacccac cgatcgatcg tcatttgccg accgataaag taaaagacgt ttacatcaag
    12361 ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt tgccaccaac
    12421 tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat gggcgaaaac
    12481 ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtaaa attaatctct
    12541 cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta cccgctctcc
    12601 agccgcttcg atgagaacga tgcgattctg gtgatggata acgtgctgat cccatgggaa
    12661 aacgtactga tctaccgcga tttcgatcgc tgccgtcgct ggacaatgga aggcggtttt
    12721 gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaattaga cttcattaca
    12781 gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg tgtacaggcc
    12841 gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga ctcgatgtgt
    12901 tctgaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc actgcaaacc
    12961 tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga acgcaacgtt
    13021 accagtggcc tgatctatct cccttccagt gcccgtgacc tgaacaatcc gcagatcgac
    13081 cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg atcacgtcca gcacatcaag
    13141 atcctcaaac tgatgtggga cgccattggc agcgagtttg gtggtcgcca cgaactgtat
    13201 gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg ccaggcacaa
    13261 agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc ggaatacgac
    13321 cagaacggct ggactgtgcc gcacttgcac aacaacgacg atatcaacat gctggataag
    13381 ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacagcgc ctgcgctttc
    13441 gtgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag ggcgacgccg
    13501 gacaatgcgg gattacggca acggccgtct gctcggtcac ggatacacca ccatcgctga
    13561 tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac ggtaagttgt
    13621 gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg ccacttcgcg ggcatgacag
    13681 gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg ctggcgcagc
    13741 cggtgctgaa aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg caggcaattg
    13801 gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca gaaggtcacg
    13861 gacttatcta ctttaaacgc cgtttccatc cagtgatgct ggaaatggaa gctgcgattt
    13921 aagctccagc cccttcacgt attcgtcgaa ggggcatttt tattgtgatc tcaatcacat
    13981 atccacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc gcttacgtcc
    14041 ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg gcaacatcac
    14101 tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac ggcttcggcc
    14161 tatgattaac aaattactct atgaattttg gtttgcagga aggcagcaag tgagtgaatc
    14221 cccgggagct tacaacagta agtgacagag gtgaacgaac gcagctgccg cacctgtaag
    14281 ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac tattcaagca
    14341 cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg
    14401 tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt
    14461 ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg accccacgcg
    14521 cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca accgttacgt
    14581 gttgtttggt caccacttcg ccgctatcgc cggtgccggt ccgctggttg gtccggtgct
    14641 cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg tagtactggc
    14701 cggtgcggtt caggacttta tggtgctgtt tatctcctct cgccgtaacg gcgcatctct
    14761 cggtgagatg atcaaagaag agatgggacc agtaccgggg actatcgcgc tgtttggctg
    14821 tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta aagccctggc
    14881 cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg
    14941 tatctacatg cgctttatcc gtccggggcg tgtaggtgaa gtttctgtta ttggtatcgt
    15001 gctgctggtt gcctctatct atttcggtgg cgtgattgct cacgatccgt actggggtcc
    15061 ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg cgtttgtttc
    15121 cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa
    15181 aatcggcgtt atcgtcggcc tggcgctggg tatcgtggta ctgaacccgg aactgaaaat
    15241 gcctgcaatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg ctctgttccc
    15301 gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc tgatctcttc
    15361 cggtacgacg ccgaaattgc tggctaacga aaccgacgcg cgtttcattg gctacggcgc
    15421 aatgctgatg gaatccttcg tggcgattat ggcgctggtt gctgcgtcca tcatcgaacc
    15481 gggtctttac ttcgcgatga ataccccgcc tgctggcctt ggcatcacca tgcctaacct
    15541 gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag acgttaccgc
    15601 acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc agatcctgca
    15661 aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggcg cgccgacgct
    15721 ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca tgggcttctg
    15781 gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg atgcgggtac
    15841 tcgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa
    15901 aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta
    15961 cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt
    16021 cggtatttcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg ttgtgctgat
    16081 taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat ggctgcttat
    16141 ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga tggaaggctt
    16201 cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc tgacggcgca
    16261 gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag gcctgagtat
    16321 tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct ggcttgcggt
    16381 gcgtaacagc gacaaacgta ctgacaaaga aactccgtac gttccaatcc cggaaggcgg
    16441 cgtgaagatc tcttcgcacc actaaccggt tgccggatgc gctgcgcgta tccggcctac
    16501 aaagtgtcta tcgtaggccg gataaggcgt ttacgccgca tccggcaaat ccctagcccg
    16561 gaccttccgg gctttacgca tatctggaag gcaaaatgtt tggtaactta ggacaggcaa
    16621 aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac gacaactacg
    16681 tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc
    16741 gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat
    16801 ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa
    16861 accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa
    16921 aacgaattcg gcgaagtctc cgttgatgat caactgattg gcgaccgcgc cacgcagatc
    16981 aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga agacgcacta
    17041 ctcgacctgc tggataatct cgacaagggc aatattcagt tcgaccgtct ggtcattgaa
    17101 tgcaccggca tggccgatcc cggcccgatt attcagacct tcttctccca tgagatttta
    17161 tgccagcgct acctgctgga cggggtgatt gcgctggtgg atgcggtaca tgccgatgag
    17221 cagatgaacc agttcaccat cgcccagtcc caggttggct acgccgaccg tattctgctg
    17281 acgaaaaccg acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac
    17341 gcccgcgcac cggtctacac cgttacccac ggcgatatcg acctgggcct tctgttcaac
    17401 accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt ccactttatc
    17461 gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc ggtagatatc
    17521 agcgaagttt cccgggtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt
    17581 tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag
    17641 cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca tagcacgatg
    17701 gtgtttatcg ggattcagtt gccggaggat gagattcggg cggcgtttgc gggactcaaa
    17761 aagtaaccag ccctccaaca tcacaatacc tgagacttta tcaccctcat attcctccag
    17821 aatatgaggg cattcgggct taattatctc tgaacgtgca acacttccta cgactcctta
    17881 tgcgcctcca catcctgctt cgttaaccag agcgacttac gaccaaaatt ttcgtacatt
    17941 ttcagctgat cttcatagtg ctgatcaact gttccatcgg gagcaataaa cccactctga
    18001 ccgggtgcga ccacatccca ggcaagcacc ggacgatcgc ttgtcgttgg tgagaaaaca
    18061 atcatatcgt tttctgttcc acggttttga tactccgcct gatgacgcgt ttcttccgct
    18121 gcggcctgcg gtacatcaaa gaaattattt gcccggaacg ttaaggccat tgcaggtgtt
    18181 ttccagttac tcacattatt gccatagcgt ttggaaagag tctcccaggt atcttccagc
    18241 gcagccaaca caacctcctg ctgtggtttc ccagcaaaca gatcaaccgc ctgtgggatt
    18301 ggtgatttgt ctccctgcac cgcctcatac aaaatttttg ctccaacact tatattcagc
    18361 gaaccagttg ggccgtcctg ggttgtttcg tagccactgg cgctgtacca cttatcaaat
    18421 ggcataggta cggcagccac tacggtacgc ttcaacatac tggtcagcca aacgttcagg
    18481 atggcagagc ctggctgctg ccaggtttta ccatcatcat taagcaaatt gatgccatcc
    18541 caacgtgtta atgtatccac caactgacgg cgcggatcgc tctgtgtcaa accagatgtc
    18601 gctgcttgca gagtaggtaa aaaaagcctc aggttaagat cctgacgact ggtttggcga
    18661 ataacatccc atgcctgatc agcagttaag cgtggctttt gctcaagcag tcggtcgatc
    18721 tccgtaacgc ga
//
LOCUS       DAJVOY010000075        17780 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVOY010000075 DAJVOY010000000
VERSION     DAJVOY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.075"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..224)
                     /locus_tag="OQL90_002220"
     CDS             complement(<1..224)
                     /locus_tag="OQL90_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCS1368985.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLR"
     gene            complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="OQL90_002221"
     CDS             complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="OQL90_002221"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1368986.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="OQL90_002222"
     CDS             complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="OQL90_002222"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1368987.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="OQL90_002223"
     CDS             complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="OQL90_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1368988.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4373..5671)
                     /gene="purA"
                     /locus_tag="OQL90_002224"
     CDS             complement(4373..5671)
                     /gene="purA"
                     /locus_tag="OQL90_002224"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1368989.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5775..5972)
                     /locus_tag="OQL90_002225"
     CDS             complement(5775..5972)
                     /locus_tag="OQL90_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1368990.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="OQL90_002226"
     CDS             complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="OQL90_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1368991.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="OQL90_002227"
     CDS             complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="OQL90_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1368992.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="OQL90_002228"
     CDS             complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="OQL90_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1368993.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETILADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="OQL90_002229"
     CDS             complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="OQL90_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1368994.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="OQL90_002230"
     CDS             complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="OQL90_002230"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1368995.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAITG"
     gene            complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="OQL90_002231"
     CDS             complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="OQL90_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1368996.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="OQL90_002232"
     CDS             complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="OQL90_002232"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1368997.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="OQL90_002233"
     CDS             complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="OQL90_002233"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1368998.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="OQL90_002234"
     CDS             complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="OQL90_002234"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1368999.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHSITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16292..17431
                     /gene="queG"
                     /locus_tag="OQL90_002235"
     CDS             16292..17431
                     /gene="queG"
                     /locus_tag="OQL90_002235"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1369000.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGGH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17414..17467)
                     /locus_tag="OQL90_002236"
     CDS             complement(17414..17467)
                     /locus_tag="OQL90_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369001.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17696..17771)
                     /locus_tag="OQL90_002237"
     tRNA            complement(17696..17771)
                     /locus_tag="OQL90_002237"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17736..17738),aa:Gly,seq:gcc)
BASE COUNT         4086 a         5036 c         4420 g         4238 t
ORIGIN      
        1 tttcttaata agaaggtatt aaattcatct ggattgctga tactacttac cggacaaata
       61 aaggtttcga taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa
      121 tcattcattt taataattaa tgcgttctcg ctatttgtta catcaatatt ttgttcaggt
      181 acagtttgca gcgccgtcgc cagcgccaac agattccatg tcatatatta ttccatatag
      241 atttagttta aatattaata aaatgaatat ttgcagtacg taattatctt accagctata
      301 gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc
      361 tgacgcaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag agaagaaaca
      421 cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata
      481 ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt
      541 gtatgatccg cctcacctgc cgtaccgacg atccagatat tctcttcctg caacatacgc
      601 atggtacgca ccaggttagt cacccgaatc agcggaacgc tttctgccgc gccacaggcc
      661 acttttttcg ccgtggcgtt gagctgggcg gagcgatctt tcggcacaat caccgcatga
      721 acaccagcgg catctgcgct acgcaggcac gcgccgaggt tatgcggatc ggttacaccg
      781 tcaaggatca gcaggaacgg ctgatcgagc gaagcgatca gatccggcaa atcattttcc
      841 tggtactgac gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt
      901 ttctcgtcga gatattggcg gtttgccagc tggataacca cgccctgggc ttcgagggcg
      961 tgaatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa
     1021 cgttcagggg cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc
     1081 attaatgtac tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat
     1141 ctctgcaata tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc
     1201 cggcatgaac aaagcgcatt ttgtcagcaa tctaaccctc ttctgtttga agagggtaat
     1261 gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc agctattttc
     1321 tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc tttcggcttc
     1381 gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgaccaga
     1441 cgacgcttgc cgcctttttt acctgcatcg ccttttttcg ctttctcgcg cgccgtttta
     1501 ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc
     1561 atattaaccg cttcgacgcg aacttccacg cgatcgccca gacgataagt ctggccgctg
     1621 gattcaccca tcagccgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa
     1681 gagacatgga ccagaccatc aatgaataag tcgtccagac ggacaaagaa tccaaagcca
     1741 gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac
     1801 ttcagccagt cagccacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag
     1861 tgctgaccca gttgcagcat ttcttccatc gaataatggt agccgccggt ttcagtggtg
     1921 ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg
     1981 tctggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag
     2041 tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc
     2101 agcatttctg catcaggacg atcggcaacc gactccagca gctccgcgta gtcacgcggt
     2161 tccggcttgt taccgcccgg cagttccagc cccagctccg ccagtactga acggaaagag
     2221 gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca atgctggttc tttggctttc
     2281 tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat taacttgtgt
     2341 gcgtcgttac gctgagtctg ttcgatacgc tcaatacggc attcagcgtt gaaaataaac
     2401 ttcgcttctt cgctctcaaa tgagatcccg ccacgttctt cacgggcttt atccagcact
     2461 ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gagcgtactg ctcgcgcaga
     2521 tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc
     2581 atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg
     2641 cacaccatac acaggcggtc tacctgcgga ttgagcgagc acagaccgtt agagagcact
     2701 tccggcagca tagggataac ctgcgaaggg aagtacaccg acgtaccacg attacgcgct
     2761 tctctatcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat
     2821 aaacgccagc cgccgccgcg ttttttctcg cagtaaaccg catcgtcaaa gtcacgggcg
     2881 tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct
     2941 tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg
     3001 tacggaattt catgggtacg caaagcgata tcaaccgcca tgccggtgcc catattgtcg
     3061 cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg ttgggtcagt
     3121 tcaacaacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata
     3181 tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag
     3241 cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga
     3301 cctttacggt cagcacccag cggctgagcc agcacctgat cgccatgaat gcaggttttc
     3361 atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag
     3421 ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag
     3481 cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc
     3541 aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg
     3601 gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc
     3661 gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg
     3721 tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct
     3781 cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct
     3841 tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat
     3901 tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg
     3961 gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac
     4021 taagttgatt gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt
     4081 cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc
     4141 cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata
     4201 catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga
     4261 aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca
     4321 atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt
     4381 cgaacgggtc gcgcagaatc atagtttcag tacgatccgg accggtagag atgatatcga
     4441 tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcagcc
     4501 cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg
     4561 gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg
     4621 gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag
     4681 tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca
     4741 gccagccggt acgacgacga cgacccgtcg ttgcgccgaa ttcgttaccc tgcttgcaga
     4801 ggaactcgcc agtttcatca aacagttcgg tcgggaaagg acctgcaccc acacgagtgg
     4861 agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg
     4921 tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga
     4981 tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct
     5041 ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg
     5101 tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga
     5161 agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt
     5221 cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac
     5281 cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt
     5341 gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc
     5401 cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt
     5461 taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg
     5521 ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa
     5581 catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt
     5641 gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg
     5701 gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc
     5761 agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca
     5821 accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag
     5881 atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc
     5941 agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca
     6001 ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc
     6061 gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc
     6121 acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag
     6181 aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca
     6241 ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg
     6301 gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc
     6361 gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg
     6421 atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg
     6481 atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg
     6541 ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg
     6601 tcacgtactt cgagggtcag acgaccacgg gaatcagtga cgatatcttt cacgtccagg
     6661 cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc
     6721 gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg
     6781 atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg
     6841 tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa
     6901 tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa
     6961 cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc
     7021 agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt
     7081 agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg
     7141 acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcaaacgc agcagattgc
     7201 tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg
     7261 gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca
     7321 ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt
     7381 ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct
     7441 tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt
     7501 cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg
     7561 catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca
     7621 gcagagtgat gcccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgggtatcgc
     7681 tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataaccc
     7741 cgcgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca
     7801 gatatttttc cggataggta acgcggtact gcacgttcat ctcaacgcgc actacgttct
     7861 cgtccgacgt cagcatcaca ccggaagcgg ccagttcacg cacggcttcc acgtttaccg
     7921 gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga
     7981 atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg
     8041 ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg
     8101 ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac
     8161 caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc
     8221 gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt
     8281 cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt
     8341 atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta
     8401 cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca
     8461 acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct
     8521 attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat
     8581 gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc
     8641 cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca
     8701 atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc
     8761 tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc
     8821 acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg
     8881 gctttaaatg ccgccaccag atcgtgcggc agatggcgaa taaaccctac ggtatctgca
     8941 agtatggttt caccgacatc cgcgacgtca atacgccgca acgtcgggtc gagggtggca
     9001 aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta
     9061 ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc
     9121 cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga
     9181 ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc
     9241 ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc
     9301 agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata
     9361 tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc caggttacgc
     9421 tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca
     9481 gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta
     9541 cggctaccgg taatcacctg caatgcttcg acaccggcgg aagaaaccag agattcaaac
     9601 tcctggaggt cttccatatc tttgtctttc gtaaaataga tgtgtaccag taccgcctgc
     9661 tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg
     9721 caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
     9781 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
     9841 gccaccggcg ttgttactgt gatgagaaac cggacgagac gggacaacag tagaaatcgc
     9901 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
     9961 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
    10021 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
    10081 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
    10141 tctcaattgt acacattcag cctgtgatag caccaacaac ctgtaatact tcgtcacgcg
    10201 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
    10261 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
    10321 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
    10381 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    10441 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
    10501 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    10561 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    10621 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    10681 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg
    10741 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    10801 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    10861 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    10921 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    10981 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    11041 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    11101 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    11161 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    11221 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata
    11281 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    11341 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct
    11401 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    11461 tcttcgccag aaacttttag ccgcaacgga ataagcagcg gctgagcgca aactggcgct
    11521 tcacccgggg tcagttgtac ctgacgcagc caacgttctg ccactggcaa ggctaaaagt
    11581 gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    11641 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttaatttt
    11701 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    11761 cctggctgcg cattcggcca aggggcagcc ggacgactgc ctgatgctgg cgcaggagtg
    11821 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    11881 acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag cggtagcggc
    11941 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    12001 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc cacctgatgc
    12061 ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    12121 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    12181 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgt
    12241 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa agcggtgccg
    12301 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    12361 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    12421 cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    12481 gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg atgcgccgcc
    12541 ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt
    12601 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    12661 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    12721 gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga
    12781 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    12841 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    12901 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    12961 tttggcagcg tgcgatctgg cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc
    13021 ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta aatggcttct
    13081 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    13141 aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc gagactcgcg
    13201 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    13261 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    13321 aaatagggat cagactgact gttcgccagc acatcgcccg ccccacccag cagctccgaa
    13381 tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg gttagagagc
    13441 acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg
    13501 aggaaattag cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga aataaagtaa
    13561 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat
    13621 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    13681 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    13741 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    13801 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    13861 gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta
    13921 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca
    13981 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    14041 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt
    14101 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    14161 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    14221 agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg
    14281 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    14341 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    14401 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    14461 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    14521 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    14581 agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa aaagccccgg
    14641 ctaaaggtgg tcttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    14701 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    14761 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    14821 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    14881 cgcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt tttgcccaag
    14941 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    15001 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    15061 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    15121 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    15181 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    15241 caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc actcttgctg
    15301 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    15361 catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttttc
    15421 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    15481 acgaatagcc cccgccgtgc cgtgatcgcc accgataatt accagccgcc cgtgatcgcc
    15541 tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa agttgttctg ctgaaaaccg
    15601 ctgaattttc gtctcctgac ctgccagcca actatccagc cccagtgagt caaaatgcag
    15661 ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt
    15721 gatggagtga tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag ccagcaggcc
    15781 ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    15841 gctaatggat tcgcggggcg cttgccgcaa accggtaccg agcagcgcat caacaatcag
    15901 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    15961 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggcaaacaa
    16021 cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt
    16081 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac
    16141 ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc
    16201 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    16261 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt
    16321 agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    16381 taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    16441 cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgccctcatg agttattgcc
    16501 cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    16561 cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    16621 tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    16681 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    16741 aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    16801 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    16861 ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    16921 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc
    16981 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    17041 gcctatctgc ccgtggaatc gctattcaca actcaccaca gaagaggatt tcagcccgcg
    17101 taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag agaagttttt
    17161 aaaagtcacg gaaggatcgg cgattcgtcg aattggtcac ctgcgttggc tgcgtaatat
    17221 cgccgtagca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg
    17281 taaaggtggg cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga
    17341 gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt
    17401 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    17461 aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc
    17521 ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct
    17581 ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    17641 acaacgaaaa ccagcaataa cgcatcgctg tggatatttt attgagagaa gaatttggag
    17701 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
    17761 gctattcccg cttgggtggt
//
LOCUS       DAJVOY010000076        17753 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVOY010000076 DAJVOY010000000
VERSION     DAJVOY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.076"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            655..1020
                     /locus_tag="OQL90_002238"
     CDS             655..1020
                     /locus_tag="OQL90_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1369002.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1060..1764
                     /locus_tag="OQL90_002239"
     CDS             1060..1764
                     /locus_tag="OQL90_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCS1369003.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGE
                     NEGVDHKVIDAFSPAG"
     gene            1831..3249
                     /gene="dcm"
                     /locus_tag="OQL90_002240"
     CDS             1831..3249
                     /gene="dcm"
                     /locus_tag="OQL90_002240"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1369004.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3230..3700
                     /gene="vsr"
                     /locus_tag="OQL90_002241"
     CDS             3230..3700
                     /gene="vsr"
                     /locus_tag="OQL90_002241"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1369005.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3689..4609)
                     /gene="yedA"
                     /locus_tag="OQL90_002242"
     CDS             complement(3689..4609)
                     /gene="yedA"
                     /locus_tag="OQL90_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCS1369006.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4782..5699
                     /locus_tag="OQL90_002243"
     CDS             4782..5699
                     /locus_tag="OQL90_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1369007.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5778..5960
                     /locus_tag="OQL90_002244"
     CDS             5778..5960
                     /locus_tag="OQL90_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1369008.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6131..7825
                     /gene="yedQ"
                     /locus_tag="OQL90_002245"
                     /pseudo
     CDS             6131..7825
                     /gene="yedQ"
                     /locus_tag="OQL90_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(7822..8637)
                     /locus_tag="OQL90_002246"
                     /pseudo
     CDS             complement(7822..8637)
                     /locus_tag="OQL90_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(8935..9151)
                     /gene="yodD"
                     /locus_tag="OQL90_002247"
                     /pseudo
     CDS             complement(8935..9151)
                     /gene="yodD"
                     /locus_tag="OQL90_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9314..9502
                     /gene="dsrB"
                     /locus_tag="OQL90_002248"
     CDS             9314..9502
                     /gene="dsrB"
                     /locus_tag="OQL90_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1369009.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9546..10169)
                     /gene="rcsA"
                     /locus_tag="OQL90_002249"
     CDS             complement(9546..10169)
                     /gene="rcsA"
                     /locus_tag="OQL90_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1369010.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10454..11239)
                     /gene="fliR"
                     /locus_tag="OQL90_002250"
     CDS             complement(10454..11239)
                     /gene="fliR"
                     /locus_tag="OQL90_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1369011.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11248..11517)
                     /gene="fliQ"
                     /locus_tag="OQL90_002251"
     CDS             complement(11248..11517)
                     /gene="fliQ"
                     /locus_tag="OQL90_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1369012.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11527..12263)
                     /gene="fliP"
                     /locus_tag="OQL90_002252"
                     /pseudo
     CDS             complement(11527..12263)
                     /gene="fliP"
                     /locus_tag="OQL90_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            complement(12263..12628)
                     /gene="fliO"
                     /locus_tag="OQL90_002253"
     CDS             complement(12263..12628)
                     /gene="fliO"
                     /locus_tag="OQL90_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1369013.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12631..13044)
                     /gene="fliN"
                     /locus_tag="OQL90_002254"
     CDS             complement(12631..13044)
                     /gene="fliN"
                     /locus_tag="OQL90_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1369014.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            complement(13041..14045)
                     /gene="fliM"
                     /locus_tag="OQL90_002255"
     CDS             complement(13041..14045)
                     /gene="fliM"
                     /locus_tag="OQL90_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1369015.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14050..14514)
                     /gene="fliL"
                     /locus_tag="OQL90_002256"
     CDS             complement(14050..14514)
                     /gene="fliL"
                     /locus_tag="OQL90_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1369016.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14619..15746)
                     /gene="fliK"
                     /locus_tag="OQL90_002257"
     CDS             complement(14619..15746)
                     /gene="fliK"
                     /locus_tag="OQL90_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1369017.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15743..16186)
                     /gene="fliJ"
                     /locus_tag="OQL90_002258"
     CDS             complement(15743..16186)
                     /gene="fliJ"
                     /locus_tag="OQL90_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCS1369018.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16205..17573)
                     /gene="fliI"
                     /locus_tag="OQL90_002259"
                     /pseudo
     CDS             complement(16205..17573)
                     /gene="fliI"
                     /locus_tag="OQL90_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            complement(17573..>17753)
                     /gene="fliH"
                     /locus_tag="OQL90_002260"
     CDS             complement(17573..>17753)
                     /gene="fliH"
                     /locus_tag="OQL90_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1369019.1"
                     /translation="LQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVA
                     TRWQELCRLAAPGVV"
BASE COUNT         4364 a         4439 c         4670 g         4280 t
ORIGIN      
        1 ctttattgcc atctttattg taaatttcag ccgcatttgc tgcgccagca actaataacg
       61 ccgggaccag cattgccaga acttttcttt tcattatgta ttcccttgtg attataatct
      121 tcatgaatat atcaataagt gccgttatcc aaaaaaaagc acatttggat actattctat
      181 gaagttcatt tcattttaag tattacatgc aacaaatgta tttaaaagat ttcaaaatgt
      241 ttctaatcta tttaatataa ttcagagata tgaattataa atattcatga aaattataaa
      301 tgtatatcaa tatgttatga gttgagcgga aacatagggg gtatcaatga ttgttgcgcc
      361 aaacggctgg tgtgatcagg cgcacattaa tgaaggcata aaaaaaagcc ggcatcatgc
      421 cggcccaaaa aatataaaca agacgataaa aggctaataa cggaagcatc atgacacaga
      481 ataataaaaa ggtataattg taatcagtgc taatatattg attaaatgaa ttaataaagc
      541 cgatatcaaa gataaataaa atcgctcaaa acgatttttc ctgtattcac tgccgttgcg
      601 caaaatttat ctatttgttc aaaaaatgat tgagtcttga ctggctcatc caatgtggaa
      661 aaatgtgact tttatcacat aatagtgcta agtctgaatt ttccgggtta tctcaaaatg
      721 gaatacggtt cgacaaagat ggaagagaga ctctctcgca gccctggcgg caaactggct
      781 ttgtgggctt tctatacatg gtgtggctat ttcgtctggg caatggcgcg ttacatctgg
      841 gtgatgagcc ggatccccga tgcccccgtg tcgggatttg aaagcgattt aggctctacc
      901 gcgggaaagt ggttaggggc actggtcgga tttttattta tggctctggt cggggcattg
      961 ttaggcagca ttgcctggta cacccgacct cgtcccgcac gtagcagacg ctatgaataa
     1021 aaactgctaa ggtggcgact ctttggcaac ggagcgcgta tggacttaca acactggcag
     1081 gcacagttcg aaaactggtt gaaaaaccat catcagcacc aggacgcggc ccatgacgtg
     1141 tgtcattttc gccgcgtctg ggctacggca cagaagctgg cggcagatga cgatgtcgat
     1201 atgctggtga ttttaaccgc ctgttatttt cacgatattg tgagcctggc gaaaaatcat
     1261 ccgcaaaggc agcgttcttc aatcctggcg gcagaagaga cgcgtcgcct gctccgtgaa
     1321 gagttcgagc aatttccggc ggagaaaatc gaggccgttt gtcatgtcat tgccgctcac
     1381 agtttcagcg cgcaaatcgc ccccttaaca acggaagcta aaatcgtcca ggatgcggat
     1441 cggctggaag ccctgggggc gattggcctg gcgcgcgtgt ttgccgtttc aggggcattg
     1501 ggcgtggcgc tgtttgatgg tgaagatccg ttcgcacagc atcgcccgct tgatgataaa
     1561 cgctacgcgc tggatcattt ccagactaag ttgctgaagc taccgcaaac catgcaaacc
     1621 atgcaaaccg caagaggcaa gcagctggcg cagcacaacg cgcatttttt agtcgagttt
     1681 atggcgaagc tcagtgccga actggcgggg gagaatgaag gtgtcgatca caaggtgata
     1741 gatgcgtttt cacccgcggg ataagcgcgt ggccctaaat ggctgtaatt atgttaacct
     1801 gtcggccatc tcagatggcc ggttaaatct atgcaggaaa atatatcagt aaccgattca
     1861 tacagcaccg ggaatgccgc acaggcaatg ctggagaaac tgctgcaaat ttatgatgtt
     1921 aaaacgctgg tggcgcagct taatggtgtg ggtgagaatc actggagcgc ggcaatttta
     1981 aaacgtgcgc tggcgaatga ctcggcatgg caccgtttaa gtgagaaaga gttcgcccat
     2041 ctgcaaacgt tgttacccaa accaccggca catcatccgc attatgcgtt tcgctttatc
     2101 gatctatttg ccggaattgg cggcatccgt cgcggttttg aatcgattgg cggacaatgc
     2161 gtgtttacca gcgaatggaa caaacatgcg gtacgcactt ataaagccaa ccattattgc
     2221 gatccggcga cgcatcattt taatgaagat atccgcgata tcaccctcag ccataaagaa
     2281 ggcgtgagtg atgaggcggc ggcggaacat attcgtcaac acattcctga acacgatgtt
     2341 ttactggccg gtttcccttg tcagccattt tcgctggctg gcgtatcgaa aaagaactcg
     2401 ctcgggcggg cgcacggttt tgcctgcgat acccagggca cgctgttttt tgatgtggta
     2461 cgcattatcg acgcgcgtcg tccggcgatg tttgtgctcg aaaacgtcaa aaacctgaaa
     2521 agtcacgacc agggtaaaac gttccgcatc atcatgcaga cgctggacga actgggctat
     2581 gacgtggctg atgcagaaga taacgggccg gacgatccga aaatcatcga tggtaaacat
     2641 tttctgccgc agcaccgtga acgcatcgtg ctggtgggtt ttcgtcgcga tcttaatctg
     2701 aaagccgatt ttactctgcg tgatatcagc gaatgtttcc ctgcacagcg agtgacgctg
     2761 gcgcagctgt tggacccgat ggtcgaggcg aaatatatcc tgacgccggt gctgtggaag
     2821 tacctctatc gatatgcgaa aaaacatcag gcgcgcggta acggcttcgg ttatggaatg
     2881 gtttatccga acaatccgca aagcgtcacg cgtacgctgt ctgcgcgtta ttacaaagat
     2941 ggcgcggaaa ttttaatcga tcgcggctgg gatatggcca aaggtgagaa agactttgac
     3001 gatccgctga atcagcaaca tcgtccacgt cggttaacgc ctcgggaatg cgcgcgctta
     3061 atgggttttg aagcgccggg agaagcgaaa ttccgcattc cggtttcgga cactcaggcc
     3121 tatcgccagt tcggtaactc ggtggtcgtg ccggtctttg ccgcggtggc aaaactgctt
     3181 gagccaaaaa tcaaacaggc ggtggcgttg cgtcagcaag aggcacaaca tggccgacgt
     3241 tcacgataag gccactcgca gcaaaaatat gcgcgcgatt gccacgcgtg atacggcgat
     3301 tgagaagcgc ctcgccagtc tgttaaccgg gcagggcctg gcgtttcgcg ttcaggacgc
     3361 cagtctgccc ggaagcccgg attttgtcgt tgatgaatat cgctgcgtga tatttaccca
     3421 tggctgcttc tggcatcatc atcactgcta tctgtttaaa gtgcctgcga ctcgaaccga
     3481 gttctggctg gagaagatag gtaaaaatgt tgaacgcgat cgccgcgata tcagtcgttt
     3541 gcaggaactc ggctggcgcg tactgattgt ctgggagtgt gcattacgtg ggcgcgagaa
     3601 gctgacggat gaagcgctta ccgagcgtct ggaagagtgg atctgcggcg aaggtgccag
     3661 cgcgcagatc gacacgcagg ggattcattt actcgcttga tgcgtcctga ataactggcg
     3721 caactacggg ttttgccggg aagagatatt ttcccaacgt gaccagtacc accgcgaaga
     3781 caattacgcc gagcgccagc cattcaatct tcgacagcgt ctctccgccc agtcccgtac
     3841 ccagcaagac cgcgaccacc gggttaacgt aggcgtagct ggtggcgaga gccggactga
     3901 cattacggat taaatacata taagcgttga tggcgataat cgaaccaaac agcgccagat
     3961 agccgaccgc aaggaagcct gaaagggaag ggagcgccgt cagtttttca cctgcaatca
     4021 tcgacgcgat cattaacacc acgcctgccg ccagcatctc aatcgcaccc gccatcatcc
     4081 ctacaggtaa ggtaatgcgc gagccataaa ccgagccaaa cgcccagcta atcgagccga
     4141 ttaaaatcag aatcgcgccc cacggattgc cgcttaaatt tccaccgcta ttgagcatga
     4201 tgattccggc aagcccaatg gcgataccca cccattccaa tttgcgcgtt ttaatgccaa
     4261 acaggcggct gaagcacagg gtaaagaggg gcacggttgc aaccacaacg gcggcgatgc
     4321 cggaaggaac attttgatgc tcggcaaccg tcaccatgcc attaccgaca gccagcaata
     4381 acaggccaat cagcgcggca ttgagcagcg gacgtagcgg ggggagcttg tgtccgcgca
     4441 gtagcaaaaa tgccatcaat aaaataccgg ctgccaggaa tcgaacgcct gccatcatta
     4501 acggaggcca gctttccacg ccaatccgaa tgacaaaata ggttgagccc caaatgatat
     4561 acaacgcaaa cagcgcgcca aaaagcggta acaactggcg gaaacgcata atccctcacg
     4621 gtggaaataa aaaggtggtt catagtaaac gtgaaaatca ttctgctggc gagagatata
     4681 attgcacttg attgttaaaa aaatgttgac ctgtgaagca agtatcagag ctgccgtttt
     4741 tgcttcatac ttacaccctt caacaataaa aatgagaggg aatgcttttg gccgggagta
     4801 gtttactgac gttgctcgat gatatcgcca cactactgga cgatatctcc gtgatgggca
     4861 aactggcggc gaagaaaacc gccggtgtat taggggatga cttatcgctc aatgcgcaac
     4921 aagtttcagg cgtgcgggcc aaccgggaac ttcccgtggt ctggggcgtg gcgaaaggat
     4981 cgctgattaa taaagtgatt ctggtgccgc tggcgctgat catcagtgcg tttatcccgt
     5041 gggcgattac gcctctgttg atgattggtg gcgcgtttct ctgctttgaa ggagtagaga
     5101 aagtgctgca tatgctggag gcgcgtaaac ataaagaaga tccggcgcag agccagcagc
     5161 gtctggagaa gctggcggcg caggatccgc tgaagtttga aaaggacaaa ataaaagggg
     5221 cgattcgtac cgattttata ttgtctgccg aaatcgtcgc catcacgctg gggattgtgg
     5281 ccgaagcgcc gttgcttaat caggtgctgg tgctttcagg catcgcgctg gtagtgaccg
     5341 tgggcgtcta cggtctggta ggggttatcg ttaagattga tgacctgggg tattggctgg
     5401 cggaaaaatc cagcgcgctg atgcaggcat taggtaaagg gttattgatc attgcgccct
     5461 ggctgatgaa agcgttatcg attgtcggca cgctggcgat gttcctcgtc ggcggcggga
     5521 ttgtggtaca tggtattgcg ccgctgcatc acgccattga acatttcgcc gggcagcaaa
     5581 gtgcagtggt ggcgatgata ttaccgactg ttttaaatct gattcttgga tttatcatcg
     5641 gcggcatcgt ggtgctggga gtgaaagccg tagcgaaaat acgcggtcag gcacattaat
     5701 actcgcacag gctattatgc aaaattcgcc atcttcaact aaggtgaaca agtttcactc
     5761 gaaaaaagga ggcgagtatg agctttatgg ttagtgagga agttacggtt aaagagggcg
     5821 gcccgcggat gattgtcacc ggatactcca gcggtatggt tgagtgtcgt tggtatgacg
     5881 gttacggggt caagcgggaa gcttttcatg aaaccgagct tgttccgggg gaggggagtc
     5941 gttctgcgga agaagtttga aagggtatca ttaacaccag gccggatcac ttccggcctg
     6001 gtgtgccaat gcctgcaatg aatgatagtc gcgtcttatc atgccaccat tataaaatta
     6061 tcgctgtttt gcggtacgct atcgccaggt tatgccagaa tcataaaaaa gcaggttggg
     6121 agtcgtcagg gtgcagcacg agacaaaaat ggaaaaccag agctggttga aaaaaatcgc
     6181 acgccgcctg gggcctggtc atgtcgttaa tctctgcttt atcgtggtat tgcttttttc
     6241 caccttgctg acctggcgtg aagtggtagt gctggaagat gcctatatct ccagtcagcg
     6301 taatcatctg gaaaacgtag ccaacgcgct cgataagcat ttgcagtata acgtcgacaa
     6361 actgatcttt ttgcgtaatg gcatgcgcga agctctcgta gcgccactgg atttcacttc
     6421 actgcgtaat gctgtaaccg agttcgaaca gcatcgcgac gagcacgcct ggcaaattga
     6481 actcaaccga cgacgcaccc tgtcagtcaa tggcgtatcg gatgcattag tcagcgaggg
     6541 gaatctcctg tctcgcgaaa atgaaagcct cgacaatgaa attaccgctg cactggaagt
     6601 tggttacttg ctgcgactgg cgcacaacac ctcgtcgatg gttgaacagg cgatgtatgt
     6661 ctcgcgtgcc ggattttacg tttcgacgca gccgaccttg tttacgcgca atgtaccaac
     6721 gcgttattac ggctatgtca cccaaccctg gtttatcggc cattcgcaat gagaaaatcg
     6781 tcaccgcgcg gtacgctggt ttacttcgca accggaacac gccagcaata ctgaaccgca
     6841 ggttaccgtc agtgttccgg tagacagtaa taactactgg tatggcgtgc tggggatgag
     6901 tattcccgtg cgtaccatgc agcaattttt aagaaacgcc atcgataaaa acctcgatgg
     6961 tgagtatcag ctctatgaca gtaagctgag atttttgacc tcttccaatc ctgaccatcc
     7021 aacagggaat atttttgatc ctcgtgaact ggccttgctg gcgcaggcga tggaacatga
     7081 cacgcggggc ggcattcgta tggacagtcg ctatgttagc tgggaacgtc tggaccattt
     7141 cgacggtgtg ctggtgcgcg tccatacgct aagcgaaggc gtgcgcggcg atttcggcag
     7201 tatcagcatt gcattaaccc tgctgtgggc gctctttacc accatgttac tcatctcctg
     7261 gtatgtgatt cgccggatgg ttagcaacat gtatgttctg caaagctcgt tgcagtggca
     7321 ggcgtggcac gacaccttaa cccgtttata taatcgtggc gcactgtttg aaaaagctcg
     7381 tccgctcgcg aaattgtgtc agacgcacca acatcctttt tctgtcattc aggtcgacct
     7441 tgaccatttt aaagcgatta atgaccgctt tagtcatcag gcgggcgacc gtgttctttc
     7501 tcatgctgcc ggattaatta gcagttcctt gcgtgcgcag gacgttgccg ggcgggtcgg
     7561 tggtgaggag ttttgtgtga ttctgccagg cgcgagtctg acggaggctg cggaagtcgc
     7621 agaacgtatt cgcctgaagt taaatgaaaa agagatgttg attgctaaga gtacgacgat
     7681 acgcatcagt gcctcgttgg gggtaagtag cagcgaggaa gccggtgatt atgattttga
     7741 acaactccag tcactggctg accgtcggct ttatctcgct aaacaggccg ggcgtaatcg
     7801 ggtatgcgcg agcgataacg cttaacgagc ggagaaaaaa tggtccagcc cttcacgcca
     7861 tccttccggt ccttcacgct gcgttcgcca gacgcgggcc ggatcctcat catgcagatg
     7921 caccccttca cggtttagcc ctttcacaat caccgcgtaa tccattacct ccagtaaggg
     7981 cgcatcgttt ggcccatcgc ccaggccaag tgtggttggg cgtttgcctg ataattgttg
     8041 ataggtcgcg ataatccagt tggcggcctg atcttttccg gcagaggcat ccaggacgtg
     8101 ccagaagcgc gcaccctgca taaactgtaa gcccagttcg ttcagacgag cggtaaattg
     8161 tgccatacgc tcgtcactgt cgcgccagat tagcgttacc gacgcctcat gcagctgcgt
     8221 cagcgccgcc tggctacggc ttaatcccgt ccattcggcg atggttgcat cgtcgacatc
     8281 atcgaaagtc gtgaatttaa aatgttcttt ctcgcgtagc gtatttaaaa cctggctgat
     8341 ttcgccatgg ctaatacctg agatgatgcg tggaaaaccg tctatatcct gccattgttc
     8401 agcaagctgg atcactgcgc cattttcggc aatcagtggt accccttgta accccaacgt
     8461 tttttgcaag tacagcattt ccgctgaagt tttactgcta cagagaatga cgggaacatt
     8521 cgcttcgcgt aaacggctga gctagggggc agccggttgc cagtcataac tatgactgtc
     8581 cagcagggtg ccatcaagat cgctaaaaac cagtagtggt tgttgaattg aaaacatggt
     8641 cgcgcagttc tccccttacc aggatgattc tcaaaacagc acaaataatc aaagatgatt
     8701 ttttcgggag cgatcccgtt gacgccagag ccaccaaggg gtgacgcacg tcacatttct
     8761 attcataagt agcgttaatc attcatatcg cgaatatttt cttgtcagcg aaaaaaattg
     8821 cggataaggt gatgaacaca tcagatttcc tggtgtaacg aattttttaa gtgcttcttg
     8881 cttaagcaag tttcatcccg accccctcag ggtcgggatt tttttattgt gcattcaacg
     8941 attcacttca gagacgctga agtcatgaat atccagttcg aaggcatccg ccaattcacg
     9001 ccatgtatga tattcacgtc catcgacgct aacgtgttca gaatcgttct ggctgcgatg
     9061 aacttatttc attgatcgcc gccagcaggg catcgacatc gacgctgacc tcacgttttg
     9121 cggtatcgct gtactctttt gcggttttca tcatctttcc tcgcaaccgt tactctgttg
     9181 ttgccgccag tcgttaagtc cgcctggcat ctcttaagta tagaccgtcg agaaaatcag
     9241 cactcgcgcg cgcctggcgc tgccagaaaa acgaaattgt tctacactgg cacaaagcca
     9301 caggaggaaa acgatgaagg tgaatgatcg ggtaacagtc aaaacggatg gcggtccgcg
     9361 tcgtcctggc gtggtactag cagttgagga gtttagtgaa ggcacaatgt acctggtttc
     9421 gctggaagac tacccgctcg gcatctggtt ctttaatgaa gcagggcatc aggacggtat
     9481 ctttgtggag aaagcagagt aatcttattc agcctgactg gtgggaaacc accagtcaga
     9541 atgtgttagc gcatgttgac aaaaatacca ttagtcacat tatccgtcag tcggacgaca
     9601 tggtagataa cctgtttatt atgcgttttg atcttacgtt taatattacc tttatgcgat
     9661 gaaacggtct tggctttgat attcatttgg tcagagattt gaatggttcc ctgacctgcc
     9721 atccacattc gcaacatact cgattcggtt cggctcaatg ataacgtcgg catatttaaa
     9781 aaagaggtta tcgttgtctc ttttttcaga atatcgccaa ggatatcgtc gagagattcc
     9841 ggtttaatcg atttagaact tatcaataaa ttttttctga ccaatagata ttcatcaaaa
     9901 tgaacattgg caattgccat aaaaacgata aataacgtat tgggatgttg attaatgatg
     9961 tgcttgatgt gctgactgtt agaagcatca tggatgaaac agtcctcatt aataaacacc
    10021 actgaagggc gctgtgaatc acaagctatg gcaaggtcat caacggtttc aatgtcgttg
    10081 atttctcttt ttttaacccc tctactcaac agatacccgg ttaaacctag tcgggtgtaa
    10141 ctacataaat ccataataat cgttgacatg gcataccctc actcaatgcg taacgataat
    10201 tccccttacc tgaatatttc atcatgacta aacggaacaa catgggtcac ctaatgcgcc
    10261 actctcgcga tttttcaggc ggacttacta tcccgtaaag tgttgtataa tttgcctgga
    10321 attgtcttaa agtaaatgtt gcgatatgtg agtgagctta aaacaaatat ttcgccgcag
    10381 gagtatcctg gaagatgttc gtaggtatta tttttcggat aatccttagg ataacatgat
    10441 aaacgttacg gaattatatt aatggcaatt cactaataat atcagccagc aaattaaaca
    10501 tctcactgaa taaatgttcg caaaaaggtg caattaacgg cattaatgcc gccattaaag
    10561 agataccgac agtcagagtt aatggaaatc caataacaaa aatggataat tgcggggcca
    10621 tacgattcag taaacctaat gccagattca gggtcagcag cagagtaatg agcggtaacg
    10681 ccagcatcag cccgttaagg aaaatcaaac tccccgcttt ggtgagtgcc agaaacgcat
    10741 tgctgttcaa cggttcgcca ccaattggca gggtgtgaaa ggtatcgacc agcagtgaaa
    10801 tcaaccataa atgaccgtta aatgtcagga acagcagtaa cgccagcata tccatgatac
    10861 gcgctaaaac gggcatattg agatggctgg ccggatcgac aaacgtcgca aatgacagcc
    10921 ccatttgcag accgataatt tcgccagcgg ttcgcacagc ggcaaaggca aattgcatgg
    10981 taaaaccaag cgcaatgccg atcaggatct gctgcacggc cagccacaga gcaaagaacg
    11041 aaaaaacagg aacatcgttg gcaggtaatg atggggcaat gacgaacgtg atcatcattg
    11101 ccagccccag ttttacccgt ttcggcacgc tgcgttcgct cagaattggt gcggtagaaa
    11161 tgagcgccag cacgcgcagt aacggccaga agtacaggct taaccaggaa agccattgat
    11221 cgcttgtcac ctgcatcatt gtacggccta cccgatgata tacggcaggt tagtgaacaa
    11281 ggtgcggacg taatccagca acagattgag catccacggt ccggcaataa taatggcaat
    11341 aaatacggcg atgatttttg gaataaacga cagcgtcatt tcgttgatct gcgtggcggc
    11401 ctgcaaaata ctgatgataa ggcccgtgac cagcgccacc agcaacagcg gggcagccag
    11461 tgccagcgcg actttcatcg cttcagtccc catcatcatg accgattcag gtgtcatttt
    11521 gcctctctag ctgtaaaagc tctgcgccag cgaaccgacc agcaattgcc agccatccac
    11581 cagtacaaac agcatcagtt taaagggcag agcaatggtg gcggggggac catcatcatc
    11641 cccaatgcca tcaacacgct ggcaatcacc aggtcgataa tcaaaaaagg aatgaaaatc
    11701 gtgaagccta tctgaaatgc ggttttcaac tcgctggtca cgtaggccgg gcgcaaaatg
    11761 cgcatcggca cggcttcagg tccctgcaac gggccggtat tcgctagtct ggcaaacaac
    11821 cctaaatctg cctcacgggt ctgacgcagc ataaactcac gtagcggctg cgcccctttt
    11881 tccagcgcct cctgcattga tattttctct tcgctgaatg gctggtacgc atcgacataa
    11941 attttgtcga tcaccggtga cataataaaa aaggtcaaaa atagtgccag ccccagcaat
    12001 acctggttag gtggcgcgga gggcgttccc agcgcgttac gcaataaacc aaaaacaatg
    12061 atgatgcggg tgaaactggt catcatcagt aaaattgccg gaataaacgt caacgaggtg
    12121 atgaacacca gcgtctgcac cgggagcgac cagctttgtc caccgccagg cagcggctgg
    12181 ctggtgatac ccggcagttg cgcgaaggcg aggggcgtaa ccagccagag aaggacaggt
    12241 gcgacagaca acaaacgacg catcaggatc tcccactacg cttaagcaaa tttttcatga
    12301 ccgactgaaa atcggtctgc ggtatctctt ctgttggtgc tgaaggggga agtttatgca
    12361 gcagattgat ttgccctgcg gtaacgccga gcaccagccg tgcatcttcc acatcgacca
    12421 ccacaacccg ttcacgcgcg cccagcgaag cactggcgct aattttcaga ccgttaacgc
    12481 cagtgcgttt cggggcaaat cccaaccgtt ttacctgcca ggcagcagcg aggatcaggg
    12541 caataatggc gatgagtgcg ccgctgacct gcagcagtgg cgcagcagaa accggcgtgg
    12601 aagattgcac agtagcgtgg ttattcatca ctaacggctc aggcgatgca ttcgctcaga
    12661 cggagtaatg atatcggtga tccgcacgcc atatttatcg gcaacgacca ccacttcgcc
    12721 ctgggcgatt aaataaccgt tgatcagaat atccagtggt tcgcccgcca gaccgtccag
    12781 cgccacgacg gacccttgcg tcagacgcaa cagctctttg atggtcatcc gcgtacggcc
    12841 cagctcgacg gtcagcttga ccggaatatc cattatcagg tcgatatcct gcaacgttcc
    12901 gctgacatca ccaccgccaa attgctggaa caccgcgtcg gcagcgcttt tgctgctggt
    12961 tgatttttgt tcgctcaacg cttcagccca cagatcgtcc attgcgccgt tgttgtcatc
    13021 ggccggatta ttcatgtcac tcatttgggc tgttcctcgt tcagagaatt taaaatcggg
    13081 ttaatcaaat gttctatccg taacgcatac tgaccgttga gagtgccgta ctgactggtc
    13141 agcaccggga cgccgtcaac atgggcgatg atgcgatcag gtttttctat cggcaggacg
    13201 tcgccgggtt tcagttttaa aatctgcgac aggcgtagcg agatatcggc aaagttggcg
    13261 accagctcca gctgtgaatg ctgcacctgg cgcaccaggt tatcgcgcca gttctgatct
    13321 tcattacgcg agttttccag cggcgggtta accaacaatt cccgtagcgg ctcgatcatg
    13381 ctgaatggca gacagatatt aaattcgccg gtcaggttgc caatctccac atggaacggc
    13441 gtgttaacca caatgtcgtt cggcgaggtg gtgatattgg taaatttcac ctgcatttcc
    13501 gaacgcacgt actcaacttc cagcggatta atcgccttcc aggcgtcgct atagccttca
    13561 agcgccagct ttaacatgcg gttgatgacg cgctgttcgg tatgggtaaa ctcgcgacct
    13621 tccactttgg tcgggaagcg tccatcgccg ccaaacaggt tatccacggc gataaacacc
    13681 agactcggtg aaaacaccac cagccctgtg ccgcgcagcg gtttcagatg gataaggttc
    13741 aggttggtcg gcaccggcag gttgcgggca aattcatggt acggctgaat gcggatggcc
    13801 ccgacggtta tatccgggct acgacgcagc aggttgaaca gccccatacg aaaatggcgg
    13861 gcaaagcgct cattaatgat ttccagcgcc tgcaaacgtt cgcgcacaac ccgtcgttgg
    13921 gtattcggat cgtacggacg aatgtcactt tcgccgctaa cactggctgt cggttcgtct
    13981 ttgacttcgc tgtcaccatt caacagcgca tcaatttcag cttgagaaag aatactatcg
    14041 cccatgtcgt tatcgcagaa taaaagcggt atacagcacg tcggtgacat cctgtttcgg
    14101 ttgcccggca acaagcgggg tggaaagcgt ggttttaatc tcggcaatca ggtttttctt
    14161 gccttcttct gtcgccagta cggcagcatc ctgacgcgaa aacagtaaca gcaaacggct
    14221 acggacttcc ggcaaatact cactcagccg cgagcgggta gcttcatctt tcaggcgcag
    14281 ggttatgccg atataaagca cgcgatccgc atcgcccaaa ttgaccgtga aggtattcag
    14341 cgcgtagaag accggggagg gtacgacgcg ttgctgcgct ttgtcgtcag cggcaacctg
    14401 atgcgaatgc cagtagctgt aacctgcgct ggcacaggcc gcgagggtaa tgaataccag
    14461 aatcgggatc caaagcgatc gcttgctttt cttgcttatc gcgtaatcag tcatgtgttg
    14521 cggtcttcct gtgtcgctac tgcttatcgg ctaattatcc tgcgttttga gcgcaacaaa
    14581 gcggggaaaa gacgcggatt acggtgctac ctctgacgtt aggcgaaaat atcaacgccg
    14641 ctgttgcctg ttacacgccc ttgtaaagag acgggaaccg gaagcgtatc gtcgtcttcc
    14701 cccgccagag gttcatggtt tactgtgcgt tggctttgct gttgttggga agcggcctgc
    14761 tgctgaccac taaagctttc gccactgatg ttgctttgcc ctaactgaat gccactttcg
    14821 gccagctgcg tacgcagtac cggaagcgct gcttccagcg cggcgcgtac atgctgatgc
    14881 ggtgaaacca tctggatttg cgcctggtta tcatccactt tgagggagat ttgcacttca
    14941 cctaaatcct gcgggtgcag acgcaactct gcactttgtt gcccctggcg ggtgaacagc
    15001 gaaatatgct ggcttaatga ttgttgccat tcgtgagaac ccagcggtgc actcaaaaca
    15061 ggcgcggcga cggtgggcag tggctgtgtc tggtgtggag taattagcgg gctggcggca
    15121 gcggtcaccg gtgacggtgt gctgatgact tccgctttac tctgggcttc tgctaccagc
    15181 ggtgttaatg gctgagctgg cgtgccgggg gcatcatcag gctgtgctgt tgtgagttgc
    15241 gcagaagtca gttttgtgaa gagcgttggt ttctctgccg gtaacacggt tgacggcaca
    15301 tcagtcactt tgggcgtatt gtcaaaaccc ggcaacatcg caaaaagggc gctcaggctt
    15361 gcggtgacgt cttcattcag atcatccgct ttttcgtctt ttgtcgtatt ttggtcagcc
    15421 accgcagcca acgtcatcgt ctgagcggtg gttaacggtg ttgatgtgga ttgttcgtcg
    15481 ttgatgacag gcagtgtttc atccacgggg atcagtaaat ccgcttgttg cgcgtcggca
    15541 agaatatcgc tgatcagcgg ctcgcctttt gtcgtgggct tatctgttgc caccagcaac
    15601 tggggggccg ctttgtcggt agttgtctcg cctgctaatg cttcgctcaa caacgcgaga
    15661 aaatcttgtg cagcatcgct ggctttgccg ccaggcaatg tggtggtgtc aacgtcggcg
    15721 gtaattaagg gcgctaagcg aatcattcag gtttcctcat ggcggcgcgc tgggcgaact
    15781 catccatctt tttctgatcg aggcggtttt ctgcaagcag tgccgccgtg gatagccgtt
    15841 cctgcagtgt ctgccaggcc tgcaaacgtt gtttcttttc tcgccagctg ttcagggcaa
    15901 tgtcaacttt ctgcgtccac tgattaagtt gctggcgatg ctgagtaatg gctttttcca
    15961 gcgtctggat aaactgctga tagttgatcc agcggttgct ggtcatcccg gcactcatat
    16021 cgctgttgag gttattgcga tattcattct gataatcgat cagcattttg agctgttctt
    16081 ccgcctgctg acacccgcga cgcatttcac ccagcaggcg cgcggcatcc tctacctctt
    16141 tttctgccag atctttcagg gtcgccagcg cgccatgttc tgccatctgc cgttatctcc
    16201 tgggttatga cactgtcggg aaaatacgct ccagcccctg gagagacgct tcccagtccg
    16261 cgcgttcaaa aatgccttgt tgcaaatagc cctccagctg cggccacagg gcgatggctt
    16321 tgtcgagcat cggatcgctg cctttggcat acgcgccgac gctaaccaga tcgcggttac
    16381 gctgaaaact cgacaacagc tgtttgaagg tgcgcactcg cgcgtaatgt tgctcactga
    16441 tcaacgccgt cattgcgcgg ctgatcgacg cttcaatatc gatagccgga tagtgcccgg
    16501 cttccgccag tcggcgagac agcacaatgt gaccgtcgag gattgcccgc gcagagtcgg
    16561 caatggggtc ctgctggtca tcgccttcag tgagcacggt ataaaacgcg gtaatcgatc
    16621 cgccgccgct aatgccattt ccggcacgct cgaccagtgc cggtaatttg gcaaacaccg
    16681 acgggggata acctttagtg gcgggtggtt cgccaatcgc cagcgcaatt tcacgctggg
    16741 ccatcgcgta gcgggtgagg gagtccataa tcagcaacac atgctgacca cgatcgcgaa
    16801 aatcttcggc aatgcgcgtg gcataggcgg caccctgcat tcgcaggaga ggagaaacat
    16861 ccgccggagc ggcaatcacc acggagcgtg cacgcccttc ggcaccgagg atattctcaa
    16921 taaaatcttt tacttcgcgt ccgcgttcac caatcaaacc cacgacaatg acatcggcgc
    16981 gggtgtaacg tgccatcatc cccagcagca cacttttacc aacgccggac ccggcaaaca
    17041 gccccatacg ctgcccacgc ccaacggtaa gcagggcatt gattgggcgc acgccggtgt
    17101 ccagcacatg ttcaatcggt gtacgttgca acgggttaaa tggcggggta atcagcgcac
    17161 cggtttccgt cgtatcgggg gaggccatcg agcggtttac cgctgccgtc cagaacacga
    17221 cctaataacg ccggaccgag cggtaactgt ttgccgcttt gcagcccttc tgccgaaatg
    17281 tttttggcat aaacacgcgc gccgggcagg acaccttcga cttcctccag cggcattaaa
    17341 aacagccgtt gaccgttaaa gccaacgact tcactttcta cttcgtgcgt ttcagtgccg
    17401 ttctggcgct caatcacaca ggttgcgccg agcggcaatt gtaatccggt ggcttccagc
    17461 accagcccgg tagcgcgggt taatcgcccg tagcgacgta ccgcaggcaa ctgcgccatt
    17521 ttggcttcaa agttatccag cgtggttagc cagcgagtca ggcgcgtggt cattacacca
    17581 ctcctggtgc tgccagacgg cagagttctt gccagcgagt ggcgacactg gcgtcgagat
    17641 cgccttcatc ggcggagact ttacagccgc caggatggag ggtgggatcg ccccgcaagc
    17701 gccagccatg caaacttaag gtagcgccga gcatatcatc cacacgttgc aga
//
LOCUS       DAJVOY010000077        17493 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVOY010000077 DAJVOY010000000
VERSION     DAJVOY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.077"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..734
                     /locus_tag="OQL90_002261"
     CDS             <1..734
                     /locus_tag="OQL90_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1369020.1"
                     /translation="KPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSDWQAF
                     NKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLH
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(805..1254)
                     /gene="rplI"
                     /locus_tag="OQL90_002262"
     CDS             complement(805..1254)
                     /gene="rplI"
                     /locus_tag="OQL90_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1369021.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1296..1523)
                     /gene="rpsR"
                     /locus_tag="OQL90_002263"
     CDS             complement(1296..1523)
                     /gene="rpsR"
                     /locus_tag="OQL90_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1369022.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1528..1842)
                     /gene="priB"
                     /locus_tag="OQL90_002264"
     CDS             complement(1528..1842)
                     /gene="priB"
                     /locus_tag="OQL90_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1369023.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1849..2244)
                     /gene="rpsF"
                     /locus_tag="OQL90_002265"
     CDS             complement(1849..2244)
                     /gene="rpsF"
                     /locus_tag="OQL90_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1369024.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2571..2846
                     /locus_tag="OQL90_002266"
     CDS             2571..2846
                     /locus_tag="OQL90_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1369025.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2921..3472)
                     /locus_tag="OQL90_002267"
     CDS             complement(2921..3472)
                     /locus_tag="OQL90_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCS1369026.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3569..4255)
                     /gene="ulaF"
                     /locus_tag="OQL90_002268"
     CDS             complement(3569..4255)
                     /gene="ulaF"
                     /locus_tag="OQL90_002268"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1369027.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4255..5109)
                     /gene="ulaE"
                     /locus_tag="OQL90_002269"
     CDS             complement(4255..5109)
                     /gene="ulaE"
                     /locus_tag="OQL90_002269"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1369028.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5119..5769)
                     /gene="ulaD"
                     /locus_tag="OQL90_002270"
     CDS             complement(5119..5769)
                     /gene="ulaD"
                     /locus_tag="OQL90_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1369029.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYQRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5783..6247)
                     /gene="ulaC"
                     /locus_tag="OQL90_002271"
     CDS             complement(5783..6247)
                     /gene="ulaC"
                     /locus_tag="OQL90_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1369030.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6257..6562)
                     /gene="ulaB"
                     /locus_tag="OQL90_002272"
     CDS             complement(6257..6562)
                     /gene="ulaB"
                     /locus_tag="OQL90_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1369031.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6578..8026)
                     /gene="ulaA"
                     /locus_tag="OQL90_002273"
     CDS             complement(6578..8026)
                     /gene="ulaA"
                     /locus_tag="OQL90_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCS1369032.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8330..9394
                     /gene="ulaG"
                     /locus_tag="OQL90_002274"
     CDS             8330..9394
                     /gene="ulaG"
                     /locus_tag="OQL90_002274"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1369033.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9502..10257
                     /gene="ulaR"
                     /locus_tag="OQL90_002275"
     CDS             9502..10257
                     /gene="ulaR"
                     /locus_tag="OQL90_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1369034.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERVGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10254..11003)
                     /gene="yjfP"
                     /locus_tag="OQL90_002276"
     CDS             complement(10254..11003)
                     /gene="yjfP"
                     /locus_tag="OQL90_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1369035.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11185..11514
                     /gene="bsmA"
                     /locus_tag="OQL90_002277"
     CDS             11185..11514
                     /gene="bsmA"
                     /locus_tag="OQL90_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1369036.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11663..11938
                     /gene="yjfN"
                     /locus_tag="OQL90_002278"
     CDS             11663..11938
                     /gene="yjfN"
                     /locus_tag="OQL90_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1369037.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12055..13680)
                     /locus_tag="OQL90_002279"
     CDS             complement(12055..13680)
                     /locus_tag="OQL90_002279"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1369038.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13764..14822)
                     /locus_tag="OQL90_002280"
     CDS             complement(13764..14822)
                     /locus_tag="OQL90_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1369039.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14825..15463)
                     /locus_tag="OQL90_002281"
     CDS             complement(14825..15463)
                     /locus_tag="OQL90_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1369040.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTISRGGYGRSSSARGHWGG"
     gene            complement(15473..15871)
                     /locus_tag="OQL90_002282"
     CDS             complement(15473..15871)
                     /locus_tag="OQL90_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCS1369041.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15889..16548)
                     /locus_tag="OQL90_002283"
     CDS             complement(15889..16548)
                     /locus_tag="OQL90_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCS1369042.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16599..17297)
                     /locus_tag="OQL90_002284"
     CDS             complement(16599..17297)
                     /locus_tag="OQL90_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCS1369043.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17370..>17493)
                     /locus_tag="OQL90_002285"
     CDS             complement(17370..>17493)
                     /locus_tag="OQL90_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCS1369044.1"
                     /translation="QKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4465 a         4672 c         4185 g         4171 t
ORIGIN      
        1 acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg tcaaatcgtg
       61 gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta gatctctctg
      121 actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga gtaacaatga
      181 atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt cacgcccaga
      241 aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat ttattgcaag
      301 atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt ttgggacctg
      361 gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc cttcctgccc
      421 aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc gaccaacttc
      481 acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg ctaaacgcgt
      541 attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat tcagcagcaa
      601 tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg agtgatcgta
      661 tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt gaatggatct
      721 acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa tggtcggcgt
      781 ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca ctttcgcgaa
      841 tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca gaacgccgtt
      901 cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag cgtcagcgat
      961 gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag acgcgatggt
     1021 aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca gaacttcagc
     1081 cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt tcttggtagc
     1141 tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt taacgtttac
     1201 ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt gcattacctt
     1261 atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc agtgtacggc
     1321 agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg gtattttgca
     1381 cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta gtttttcagc
     1441 gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg gcagaacttg
     1501 cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt caatctgctc
     1561 ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa tgaacccctg
     1621 aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt cgtgtccgct
     1681 aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct cctgcacaga
     1741 acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct ttcgaagggg
     1801 agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt actcttcaga
     1861 atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac gctcacggcg
     1921 ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg tacgcataac
     1981 catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat cgatcacttc
     2041 ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt tgatcgggta
     2101 agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg caccagtgat
     2161 ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag gatggaccat
     2221 aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag cctcctgtct
     2281 gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa aaatgacgcg
     2341 ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat ctcatctttc
     2401 gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag tgttttttcg
     2461 aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa attaaatcag
     2521 aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct atgttcagtc
     2581 gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca gccattgaaa
     2641 ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg atttatatca
     2701 atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag acagatgcgc
     2761 agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac ggcaatatgc
     2821 acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat tgccatagtt
     2881 tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt gcgacttccg
     2941 gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct attatccaga
     3001 accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat tgagtgagcg
     3061 gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc acaataagcc
     3121 ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca atacaatacc
     3181 ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg ataatatgtt
     3241 ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt agaaagatat
     3301 acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag gggacttccg
     3361 ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt aacgcaacgc
     3421 catagcggcg acgttgcgga tccgttctga caaacaatcc attgatagcc ataaaaacac
     3481 ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat tacatcccgc
     3541 aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg ttaggaccgt
     3601 gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt tgtgggttaa
     3661 tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg ttatgcaccg
     3721 cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca attcccggcg
     3781 tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg ttcagttcat
     3841 actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg ccaaagaagt
     3901 agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc catgcggtgg
     3961 catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg tagagttcaa
     4021 gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg ccgctcatat
     4081 cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg ggcttgatca
     4141 ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag gtcaccagcc
     4201 catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt tgcattatgc
     4261 cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg ctttcaccac
     4321 ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg gcccgcaata
     4381 gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt cgccaaatgg
     4441 cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga tatgcccgat
     4501 tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc cgatatctgg
     4561 gtagagctgg aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct tgctgatgga
     4621 gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc ggctcgccat
     4681 ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat tggcttcctg
     4741 atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct gggcgaactg
     4801 gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt cgctgcccaa
     4861 cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg tttcaacaat
     4921 cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca ggcgctcgtc
     4981 agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca gtcgcaggcg
     5041 ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg ggatttgttt
     5101 ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga actgacgcgc
     5161 ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca catgaattgg
     5221 aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg tgactttgaa
     5281 gcccatatcg gaaagacgtt tgatcgcagt gatatcggct tcaccccacg ccacgccagc
     5341 ggcctgcgcg tcacggctgc gctgataaac cacctgctga atacctgcgt cgcgccactg
     5401 ttgcgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc cgttaaactc
     5461 ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa tcacggtcac
     5521 ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat cggcaatttt
     5581 ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa ccgcacgcac
     5641 gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt cggcaatcag
     5701 gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt gcaacatcgg
     5761 taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg agatccagta
     5821 cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct tcaaacaggt
     5881 tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc gccatggtga
     5941 tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt ttcagcgtca
     6001 ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc attgccaggc
     6061 ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc gcctggtagt
     6121 aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt actgcgtcct
     6181 gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc gaatcacgta
     6241 atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc acttccagca
     6301 gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg tatttgttgc
     6361 cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc gcgccgctca
     6421 actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg tttgattggg
     6481 ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg ttgccacaca
     6541 cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt tctgccagtt
     6601 gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata taagccagtg
     6661 caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc tgcatcatcg
     6721 gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg gtgagtttca
     6781 ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc ttcagcgccg
     6841 cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag aagaacatcg
     6901 ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg ccaaccgcaa
     6961 tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc gcgaagctat
     7021 agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg gagataccgt
     7081 taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg atgaagatcg
     7141 ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact ttgcctgcca
     7201 tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag atggtcatca
     7261 caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc agtttgaggt
     7321 cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca atccatgatg
     7381 caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc tgagtcggct
     7441 tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc gtgcagataa
     7501 tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc cccgcctgct
     7561 ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga cgcagcagca
     7621 cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag ctataggcat
     7681 cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt gcgccgttaa
     7741 tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg aggatgccgg
     7801 accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta ataataacgc
     7861 tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc aacaacgggg
     7921 cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga tctcctgaag
     7981 aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta attttcaaaa
     8041 gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata attccatacg
     8101 gatagagtgg gatccacaca acgaaacaat tatttattta aatttagtta aattcattta
     8161 gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca acaacttccc
     8221 tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag gattaatcaa
     8281 aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga tgagtaaagt
     8341 gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg gtagctggct
     8401 gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt ggtggctagg
     8461 ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg atttctggtg
     8521 cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc agatgcagcg
     8581 catggctggc gtgaaaaaac tgcagccaaa cctacgcacc accccgtttg ttcttgatcc
     8641 gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg atcatatcga
     8701 cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat tcattggacc
     8761 gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt gcatcgtggt
     8821 caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg atgctttcga
     8881 ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac tgccggacgg
     8941 catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc tgtatcacag
     9001 cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc agatcgacgt
     9061 ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga ccagcgccga
     9121 tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc accacgatat
     9181 ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga tgaaaaaaga
     9241 tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat ttacctggcc
     9301 actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt gcttcactat
     9361 tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg gatagcgtgg
     9421 agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat ctaaaaaaat
     9481 caaattttat cggaatagct catgactgaa gcacaaagac atcaaatcct cctggaaatg
     9541 ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg aatttcgcct
     9601 gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa aaaggtgcgc
     9661 aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa tctgcatcag
     9721 gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct ggttaatccg
     9781 ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg ggaaatgtgt
     9841 ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct gatcgatcaa
     9901 gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc catcacttta
     9961 agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac cagcggaaaa
    10021 gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc agagcagaag
    10081 atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat tggcgaacgc
    10141 gtgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg caaaaatgct
    10201 aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg tgtttaaaga
    10261 tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg gtgacgcacg
    10321 cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa ggcctgctgc
    10381 aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca cagcagtaga
    10441 ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag cggcgcgata
    10501 atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg aaacagtgaa
    10561 cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt caccgtgggg
    10621 tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc gactgccaga
    10681 cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt gaattcctgc
    10741 atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc gctactaaaa
    10801 cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg cgccagcgca
    10861 acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa aattacgcac
    10921 ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat atctgccagc
    10981 tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac atgaagaaat
    11041 ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg ggttacgctt
    11101 tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca ttcccccaaa
    11161 gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt tatttaccgg
    11221 tttgccagtt tattattggt gttgatgtta agtgcctgta gcgcactgca aggtacgcca
    11281 cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca gacgcaaggg
    11341 ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga cgcattagca
    11401 gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt catggtggac
    11461 gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa ataaatcaga
    11521 caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct gtgcacaata
    11581 aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc aacatgtggg
    11641 gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc tgcaattagt
    11701 gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg actgcgtgac
    11761 gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc cgcaggacgt
    11821 ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc gcattattac
    11881 catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt atgcatgagt
    11941 gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc ggcctacaat
    12001 tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat acgcttacac
    12061 acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg acaaccgtac
    12121 gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg gagaagcata
    12181 tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct ctcgccccag
    12241 ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat caaaatagcg
    12301 atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc ccgcttgctt
    12361 attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc aaatactgtt
    12421 taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac caatgccgcc
    12481 cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca caaattttgc
    12541 cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacgcc ggtcccacgc
    12601 tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca tgcgacttaa
    12661 gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc gttgatgtgc
    12721 atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca gggcgcaatc
    12781 aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt cctgccccaa
    12841 caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc gattacccag
    12901 cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca aaaagcgcgg
    12961 cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg catcgctttg
    13021 cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt ccagacgctc
    13081 tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca ttcccatgcc
    13141 aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc gatcgctcag
    13201 cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca atggcgtggc
    13261 ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat gtaacataaa
    13321 acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg caagattgtg
    13381 cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa aacgcacatc
    13441 gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca cattcgccag
    13501 ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg ctagaaaatc
    13561 gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat cagacaggta
    13621 taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt gccagtgcac
    13681 tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg gattcctgac
    13741 agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa acgtgaggtg
    13801 tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc atcatcgaca
    13861 atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg aaacgcctgg
    13921 tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt attctgaccg
    13981 tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac atagctttcg
    14041 ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt aggatgacca
    14101 ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact tttccataac
    14161 ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc acgcatcatc
    14221 cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag atcggtcagt
    14281 acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc ctgccctgcc
    14341 tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt gtcctgacag
    14401 cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat caggcgttcc
    14461 tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc aaaatccatc
    14521 cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc ccagtacaac
    14581 ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc caccacctca
    14641 aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat ctggcgcaga
    14701 gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat atgaaagtcg
    14761 aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt gtgtctcagc
    14821 atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg ccgcgcgaaa
    14881 tggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt ttgccagaac
    14941 cggagcgcca ggagtaatca ccggaagtgt tgagccagac ggggcgggaa gcaaatgagg
    15001 atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc cggcttaaca
    15061 aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag caattttcgt
    15121 acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa aatgccgctt
    15181 tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca atacaatcct
    15241 gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta tcgccacaac
    15301 ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat ttttgtgcgt
    15361 agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg atcgctccgt
    15421 gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc cattaccatg
    15481 tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata aacattcctg
    15541 ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt ctgacaccag
    15601 caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag tctggaatac
    15661 tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat gtaccgctga
    15721 atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg tgcggtgtaa
    15781 ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa aaataggcac
    15841 taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt aaccaataat
    15901 atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc actctggctc
    15961 gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag tatcttcggt
    16021 tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat ggttttgatt
    16081 ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc tgttaaaaaa
    16141 tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt tggcgttgat
    16201 cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga caaagagctt
    16261 gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca gaaattcatc
    16321 gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc ggctgacagc
    16381 ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca gcaaacgaaa
    16441 cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac cgcgagcgat
    16501 agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat tttatcctta
    16561 aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg ttggcgttgc
    16621 agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc ggcttccgcc
    16681 agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc agcatccagg
    16741 cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc cgctgtcgaa
    16801 acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg ctgcatggct
    16861 tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg cgctgtcgct
    16921 gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac ttgctcttca
    16981 gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat caacgacgga
    17041 ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt gcgctcacgt
    17101 aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac gcgagatttt
    17161 ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat gcgcacgccc
    17221 tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag cgtaaataaa
    17281 ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc ttctgtaatt
    17341 tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat atcttcaaga
    17401 gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg cacgctggag
    17461 attcctaccg atgacaacgg catcatcttc tga
//
LOCUS       DAJVOY010000078        17342 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVOY010000078 DAJVOY010000000
VERSION     DAJVOY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.078"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            189..2036
                     /gene="ldtD"
                     /locus_tag="OQL90_002286"
     CDS             189..2036
                     /gene="ldtD"
                     /locus_tag="OQL90_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1369045.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2217..2765
                     /locus_tag="OQL90_002287"
     CDS             2217..2765
                     /locus_tag="OQL90_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1369046.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2792..3439
                     /gene="gloC"
                     /locus_tag="OQL90_002288"
     CDS             2792..3439
                     /gene="gloC"
                     /locus_tag="OQL90_002288"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1369047.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3661..4851)
                     /gene="aspC"
                     /locus_tag="OQL90_002289"
     CDS             complement(3661..4851)
                     /gene="aspC"
                     /locus_tag="OQL90_002289"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1369048.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5036..6124)
                     /gene="ompF"
                     /locus_tag="OQL90_002290"
     CDS             complement(5036..6124)
                     /gene="ompF"
                     /locus_tag="OQL90_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1369049.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYAHLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="OQL90_002291"
     CDS             complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="OQL90_002291"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1369050.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8295..9497)
                     /gene="pncB"
                     /locus_tag="OQL90_002292"
     CDS             complement(8295..9497)
                     /gene="pncB"
                     /locus_tag="OQL90_002292"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1369051.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9763..12375
                     /gene="pepN"
                     /locus_tag="OQL90_002293"
     CDS             9763..12375
                     /gene="pepN"
                     /locus_tag="OQL90_002293"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1369052.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12418..13169)
                     /gene="ssuB"
                     /locus_tag="OQL90_002294"
                     /pseudo
     CDS             complement(12418..13169)
                     /gene="ssuB"
                     /locus_tag="OQL90_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13166..13903)
                     /gene="ssuC"
                     /locus_tag="OQL90_002295"
                     /pseudo
     CDS             complement(13166..13903)
                     /gene="ssuC"
                     /locus_tag="OQL90_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13968..15113)
                     /gene="ssuD"
                     /locus_tag="OQL90_002296"
                     /pseudo
     CDS             complement(13968..15113)
                     /gene="ssuD"
                     /locus_tag="OQL90_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15110..16069)
                     /gene="ssuA"
                     /locus_tag="OQL90_002297"
     CDS             complement(15110..16069)
                     /gene="ssuA"
                     /locus_tag="OQL90_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCS1369053.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16062..16637)
                     /gene="ssuE"
                     /locus_tag="OQL90_002298"
     CDS             complement(16062..16637)
                     /gene="ssuE"
                     /locus_tag="OQL90_002298"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS1369054.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            16993..>17342
                     /locus_tag="OQL90_002299"
     CDS             16993..>17342
                     /locus_tag="OQL90_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369055.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAA"
BASE COUNT         4371 a         4435 c         4398 g         4138 t
ORIGIN      
        1 acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta tcggcgttta
       61 tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg tgagtattgg
      121 cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat aaaaacaggg
      181 ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc agtttgtgcc
      241 tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa gtaatccctg
      301 gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg caagccacgg
      361 caataatggc ggggatccag ccattgcctg aaggtgcggc agaaaaagcc cgcacgcaaa
      421 tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt caactgttgt
      481 atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa gccttccagc
      541 aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa tgggtagagt
      601 tactgaccga tcctggtgtt aacgggatgg cacgcgacgt agtgctctct gatgcgatga
      661 tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg ctatatagca
      721 gtaaacctta tgcacttgca acgccgccgc tctcggtgat taaccaatgg cagctggcgc
      781 tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat ccgcaatatg
      841 cggcgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg ccccaactga
      901 ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg ttgcgcgaaa
      961 tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct ggcgatgaca
     1021 cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca gaaactaagc
     1081 cgatggacaa gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgc gccgcctacg
     1141 ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg ggggcagatg
     1201 gtgctattgg cccggcaaca cgtgactggt taaacgtaac gcccgcccag cgtgctggcg
     1261 tgttggctct caacatccag cgattgcgct tgctgccaac agagctttct accgggatca
     1321 tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag gtgctggatt
     1381 cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt gcccttaaca
     1441 acgtagtggt aaacccgccg tggaacgtac ctccaactct ggcacgcaaa gatattctgc
     1501 caaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg atgcgtggct
     1561 ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc acggcctcga
     1621 atttaccgtt ccgcttccag caggctccag gcccacggaa ctcgctgggg cgctataaat
     1681 tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac aatctgttca
     1741 agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct tccgatctgg
     1801 cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat gcgctgaagc
     1861 agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc tactacctga
     1921 cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac aattatgatc
     1981 tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc aggtaaatga
     2041 agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggcga ttctctgcag
     2101 cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg ttaaggtgcc
     2161 tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg attatcatgg
     2221 acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca ctcggtgccg
     2281 ccatcctgcc gacccctgcg tttgcaacac tctctacccc acgcccgcgc attttgacac
     2341 tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc agaggctata
     2401 ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg aacaaaataa
     2461 agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggttg ttaggcacgc
     2521 gcaaaccggt gcaactcatt tccggttatc gttccattga taccaacaat gaactacgcg
     2581 cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg atggatttcc
     2641 atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg cgcgcaggtg
     2701 gtgtaggata ttacccacgt agtaactttg tgcatattga taccgggcca gcacggcact
     2761 ggtagcaatc gcttaacgaa acaggggcag tatgaactat cgtattattc cggtcaccgc
     2821 attctcccag aactgttcat taatctggtg tgaacaaacc cgtctggccg cactggtcga
     2881 tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc tgacactgat
     2941 gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg aactggcgca
     3001 acattacggc gtgccggttt ttggcccgga aaaagaagat gagttctggc tgcaaggctt
     3061 gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc cagatcgttg
     3121 gctgaacgaa ggcgatacca tcagcatagg aaatgtgact ttacaggtgt tacattgccc
     3181 tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc tgatttctgg
     3241 cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg atcataatca
     3301 actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga catttattcc
     3361 gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct tcctgcaaga
     3421 cgaaatgccc gtctggtaag gcacataaaa aagcccgctt ttaatgcggg cctggatttc
     3481 tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg gcctacaaaa
     3541 tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag cgcatcaggc
     3601 aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg tttttaatgc
     3661 ttacagcact gccacaatcg cttcgcacag cggagccatg ttatctggtg tcatcccggc
     3721 cacatttacg cgaccagaag caaccgcata tacgccaaac tcttcgcgca gacgcagcac
     3781 ttgttctttt gtcaggccac tgaaggagaa catgccgttc tgtttgatga taaagctgaa
     3841 gtcgcggttt gcgccttttt cctgcagcgt attgacgaac aactgacgca tacgctgaat
     3901 acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaacgcat cgttgctcag
     3961 gatggtggca acaacagaag cgccgtgtgc tggtgggtta gagtagttag cgcgaatcgc
     4021 cgctttcatt tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa ccagagtaca
     4081 agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg caacaatcag
     4141 ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca gaccacgggc
     4201 aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg agagttgtgc
     4261 cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc agcagccatg
     4321 gaacagcact acgtcgccag cctgagcttc attcaggctg ttaatcagtg catcgaagtc
     4381 aagagtgtga ttttccgcat cataataagc gtattcacga acttccagac ctgcagagtt
     4441 aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacactcgct taacgctggt
     4501 attttttgcc aggaaatcgg cagctacgcg tagtgcgcca gtgccccccg gagtctgtgc
     4561 catgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca gttcctgagt
     4621 gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg tttcattttc
     4681 gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc ccgtctcatc
     4741 tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac gaaacagatc
     4801 ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca tgacgaggtt
     4861 ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta caaaattcca
     4921 actattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc tggaaggtat
     4981 aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa caaagaggtg tgctattaga
     5041 actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt ttgttatcag
     5101 aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg aagtagtagg
     5161 ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct acgtctttcg
     5221 ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg tattgcgcaa
     5281 ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat ttattagtga
     5341 tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg ttcgcgtcgt
     5401 acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt tgagcttctt
     5461 gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct tcgtattcgt
     5521 agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca cgctcgtttt
     5581 tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag ttggagttac
     5641 gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat gcagtatcac
     5701 caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca ccgtagttac
     5761 ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc agacgcgttt
     5821 tgttaccagt ttgagcgtca gcgccttcag agttgttacc ttggaagtta tattcccact
     5881 gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca agatgggcat
     5941 aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa aaataatgca
     6001 gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag atttctgcag
     6061 cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata ttgcgcttca
     6121 tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctgcaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc
     6361 atatttccat agaaacgtga tgatatttac agcaattttt atctatttat atgatttact
     6421 tatatttaaa ttaactaagc ggaaattttg tttctgatgg aaactttatc gacctggcac
     6481 aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt gctatcagct
     6541 aagaaaaaac taatccgcaa taagaatacg ggttattttt tgtgcgtaaa tgttgcaatg
     6601 taatattccg taacaggatg atcgttatgg cggggttgca aatagattgc ttgacttaac
     6661 aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct ctttgaggaa
     6721 gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc acgcacgttt
     6781 tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc tgaatgcgga
     6841 acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt caggcccatt
     6901 tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc tccaatgatc
     6961 tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc ttcgttgagg
     7021 cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg tgctttaaag
     7081 tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca gtaaaccggg
     7141 ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta atccacctgc
     7201 gcgaaatcgg cctcaatgaa gcgttccaga cgtgaaacgg catctttatc tacgcgttca
     7261 gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac atatttcagc
     7321 atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc cggctccagc
     7381 atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa agtcgggccg
     7441 aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg gccagatacg
     7501 gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc ctgatcgtta
     7561 cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc accttcggta
     7621 tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt aaagaagcga
     7681 tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag gtttgtgcgc
     7741 ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc cgccatcggg
     7801 taagtgtctg gatcttcaac ccaaccagca acttcaactt tgctggcctg aatttcaaat
     7861 tgttgcccct ggcccggcga cgccacgact ttacccgtca caatgaccga gcagccggtg
     7921 gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac agcctgtaca
     7981 ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt tgaatctcgg
     8041 cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg gccctggagt
     8101 acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg aaaaaataaa
     8161 cacttgtcca cccgtaaatg gggggatacc tatgttacct ggcatctgca atcagacaag
     8221 cagaattcgc aaatgcagcg aaagatttcg gaattaaagg taaaaaaagg gagctgatta
     8281 gctccctgat gatattaact ggctttttta atatgcggaa ggtcgaacgc tttgcgcagc
     8341 gcccgaacaa acgctttatc atggcagata gttttgccag ggctgtcaga aagtttcgcc
     8401 accggtttac cgttacactc taccaactta atcacaatat tcaggggttt tacctggggg
     8461 atatcgcagg tcaggcgagt cccaatacca aaacttaatt gcacgcggga agagaagtgg
     8521 cggtatagct caaccgcttt gcgtaaatcc agattgtcag agaaaaccag cgttttactc
     8581 tgtggatcaa ttcccagctt tttataatgt gcaatggctt tttcacccca ttcaaccggg
     8641 tcgccagagt catgacgcag gccctgatac cgactggcga actcgacacc gaaatcacgc
     8701 aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc caagttggtc gggatattct
     8761 tccagccagg cagcaagtgc agctcgctgg ctgttggcta ggtccgggct gatttgctga
     8821 tgtgcctgga accattcgtg cgcctgtgtt cccatcggcg tgagggaaag ccgacgcgcc
     8881 agatcgtagt tgctggtgcc cacgaaccag gattcctgtt gcagacgctt aacgatggtt
     8941 tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat ccatcagatg gaagcgcgac
     9001 atatcaagac cggcggttaa cgccgagaag tcgactaatt tgctttccag cgtgtcgagg
     9061 gcttgcgcaa cgtcggcctg cggtgagcga tagcgatgta ccatttcact gataaccgcc
     9121 agcaaaggaa cttcccagag gatgacttca cgccacggac cgcttaagcg aatatccagc
     9181 ttgccattat cgttggacac ggtgacttgt tccgggttaa agcggaattc gcgtaaccag
     9241 ttaagatagt cggccttaaa gaaaggcagg gcagagagcc actgatattc atcatcctgc
     9301 aggcgcaggt gctgcatcgc ctgaatctgt tcacgaatag catcggcata aatacccagc
     9361 agatcgtcac ctcggcaacg aaactccgcc gcgacatgca catcgtaata gtgatgaaac
     9421 acggcttgct gcatatgcaa cttataagca tctgtatcca gcaacgagtg cagaacagga
     9481 gaagcgaatt gtgtcatagg tgcgcagtag catcctcgta caggagcgtt tagtacaata
     9541 aacatcttca ggaactgctg gagtatacct tgtttcgcaa tttattgaac cccgatcaca
     9601 ccatatgcca cctttctggt cgatggcatt acgcggtgta tgttataaaa atgtagcaat
     9661 aaaggcgttt gtacctgaaa agatgaagat tctgcatagc gcgatttacg caacaggaat
     9721 agactgaaca ccagactcta taaaagatgc taaaggttat ttatgactca acagccacaa
     9781 gccaaatacc gtcacgatta tcgtgcgccg gattaccaga ttactgatat tgacttgacc
     9841 tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt ccgtcatggt
     9901 gcatcagatg ctccgcttcg tctcaacggc gaagacctca aactggtttc tgttcatatt
     9961 aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg cactggtcat cagtaatttg
    10021 ccggagcatt ttacgcttaa gatcgttaat gaaataagcc cggcggcgaa taccgcgctg
    10081 gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga aggtttccgc
    10141 catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac caaaattatt
    10201 gccgataaaa ccaaatatcc cttcctgctt tccaacggta accgcgttgc gcaaggtgaa
    10261 ctggaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc gtgctacctg
    10321 tttgcgctgg tggcaggcga ctttgatgta ctgcgcgaca cctttaccac gcgttctggt
    10381 cgcgaagtgg cgctggagct gtacgtcgat cgcggcaacc ttgatcgcgc gccgtgggcg
    10441 atgacctcgc tgaaaaactc aatgaaatgg gatgaagaac gcttcggcct ggagtatgac
    10501 ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat ggagaataag
    10561 ggtttgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc cactgacaaa
    10621 gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg gaccggtaac
    10681 cgagtcacct gccgcgactg gttccagctc agcctgaaag aaggtttaac cgtcttccgc
    10741 gatcaggaat tcagctctga ccttggttcc cgtgcagtta accgcatcaa taatgtgcgc
    10801 accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc gatccgcccg
    10861 gacatggtca ttgagatgaa caacttctat accctgaccg tttacgagaa gggcgcggaa
    10921 gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg gatgcaactc
    10981 tatttcgagc gccatgatgg cagcgcggca acctgcgacg actttgtgca ggcgatggaa
    11041 gatgcgtcga atgtcgatct ctcccatttc cgccgttggt acagccagtc cggcacgccg
    11101 attgtgaccg tcaaagacga ctacaatccg gaaaccgagc agtacaccct gaccatcagc
    11161 cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat tccgtttgcc
    11221 atcgaattgt atgacaacga aggcaaagtg atctcgttgc agaaaggtgg tcatccggtg
    11281 aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa tgtctacttc
    11341 cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga atataagtgg
    11401 agcgatcagc aactgacctt cctgatgcgt catgcgcgta atgatttctc ccgctgggat
    11461 gcggcacaaa gcctgctggc aacctacatc aagctgaacg tcgcccgaca tcagcaaggg
    11521 cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcagtgct gctcgatgag
    11581 aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa tgaaatggct
    11641 gaactgttcg atatcatcga cccgattgct attgccgaag tacgcgaagc actcactcgt
    11701 actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa ttaccagagc
    11761 gagtaccgtg ttgagcatga agatattgcg aaacgcactc tgcgtaatgc ctgcctgcgc
    11821 ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca gttccacgaa
    11881 gcgaacaata tgaccgatgc gctggcggcg ctttctgcgg cggttgccgc acagctgcca
    11941 tgccgtgatg cgctgatgca ggaatacgac gataagtggc atcaggacgg tctggtgatg
    12001 gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga gacggtgcgc
    12061 ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg ttcgttgatt
    12121 ggcgcgtttg cgggcagcaa tccggcagcg ttccatgccg aagatgggag tggttaccag
    12181 ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtggc ttcacgtctg
    12241 attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa aatgcgcgcg
    12301 gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctgtacga gaagatcacc
    12361 aaagcactgg cttgataaat aaccgaatgg cggcaatagc gccgccattc ggggaattta
    12421 cccctgtttt ctcaggcgaa tttcagattc accacgctgc atcacccttt gcaacacttc
    12481 cgattccagc tccgctaatc gcaccgaccc taatcgacgc ggagggggga tatccactgt
    12541 caaatccaga ccaatttttc cctcttcaat taataacacc cggtcagcca ttgctaccgc
    12601 ttcgctcaca tcatgcgtca ccaacagtac ggtaaagccg tgctcctgcc aaagtgacac
    12661 aatcaaatcc tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag
    12721 caacaataaa cccggtcgat gaatcaacgc tcttgccagc accactcgct gtttctgccc
    12781 gcctgaaagt gcggcaggcc attccccggc gcgattctcc agtcctaccg cagccaacgc
    12841 ccgacgcgcg gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga
    12901 tttccacggt agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatttcagc
    12961 cagcggtgtg gtgcctgcca acacatcgcc tgcggttggc gtttccagcc cggccagcag
    13021 gcgcagcagg gtacttttgc caccaccgcc acaaactgac ctgccggaat atgtaaatcc
    13081 agttggttca ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag caacaatggt
    13141 gtgccctggt tcagacgagc agtattcata ccgtggcctc cttcaaatga taagccgggt
    13201 tccagcgcaa ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt ttgccgagca
    13261 gggcgtaaag aataatagcg actaccacca cgtccgtttg caaaaactct cgcgcattca
    13321 tcgccagata accaatgccg gaattggcag aaatggtttc ggcaacaatc agcgtcagcc
    13381 acatcaggcc caacgcaaaa cgcacgccga ccataattga gggcagggca ccaggcagga
    13441 tcacatggat aaacagcggt atgccggata atccatagct acgcgccatc tccaccagcc
    13501 cgcgatcgat attacggatg ccatgccagg tgttgatata aatgggaaac agcgtaccga
    13561 gcgccaccag aaagattttt gcggattcat caatgccaaa ccacaaaatc accagcggga
    13621 tcagcgccag atgcggcacg ttgcgcaaca tctgaattga ggtatccagc agccgttctc
    13681 cccagcgaga tagcccgcta atcagtccca aaatcaaccc cagcgatccg ccaattgaaa
    13741 agccaatcag cgcccgctag gaactgatcg ccagatgctg ccacagttcg ccgctggcgg
    13801 agagcgtcca gaacgccgtc accacccctt ccggtgaggg caaaatacgc gtcgacagcc
    13861 agccaactga ggaggccagt tgccacaccg ccacgatgcc catcggtaaa aactaggggg
    13921 caacgcgcaa taaccacttc ttcactggcg ttgccattat ttcttcctta gctttgcgcg
    13981 actttacggg ggataaaatc attcgccacc gcttcgcctt gcggattcag cggctgcggc
    14041 tggggaattt ccggaatggc gacatccaga tgtgggaaca gcaactcgcc gacccgatac
    14101 gcttcttcca gatgcggata gcccgaaagc acaaagctgt cgatgccaag cgcggcgtat
    14161 tcgttgattc gcgcagcgac cgtaggacca tcgcccacca gcgccgttcc ggcaccgccg
    14221 cgcactaagc caacgcccgc ccataaattg gggctgatct ccagattgtc gcgcttgccg
    14281 ttatgtaacg ccgccattcg ctgttgtcct acggaatccg tccgggcgaa tgcggcctgt
    14341 gctttggcga tagtttcatc atcaagatgc gagattaacc gctcggcggc ctgccacgct
    14401 tcgtcgttag tttcacgaac aatcacatgc agacgaatac cgaaacgaat tttgcgtcca
    14461 tgcgcggcag ctttcgcccg cacttgttcg atcttctctt ttactagttc tggcggttcg
    14521 ccccaggtga ggtagagatc aacttgttct gccgccagct cctgggcgac gtctgacgat
    14581 ccgccaaagt aaagcggagg ataaggctgt tgaatcggcg ggaagagcag ttttgctccg
    14641 cgcacatgaa tatgtttacc gttgaaattg acggtttccc ccagcaataa gcgccgccag
    14701 acctaggtaa attccgccga ggcttcgtag cgctcgctat gatcaaggaa cacgccgtcg
    14761 cctgccagct cttgtggatc gctgcctgtg accagattaa acaacgcacg accatttgag
    14821 agacggtcaa gcgtggcggc ctggcgagcg gcaacggtag gtgaggttac gctggggcgc
    14881 agggcgacaa gaaacttcag ccgctgcgtc accgggatca tcgacgccgc caccagccac
    14941 gcatcttcac acgatcgccc cgttgggatc agcacaccgg tatagcccag acgatccgcc
    15001 gcttgcgcaa tttgttgcag ataaccgtga tcaaccgggc gtgaaccttc ttccgttccc
    15061 agatagtgcc cgtcaccgtg ggtcggtaaa aaccagaaca tattcagact cataattgtt
    15121 ttccttccag ttgagtgggc tgccagatgc gctggcgaat atcgactttt ttcggcacca
    15181 gacgattttc ataaaacaga tctgccgttt gctgctgtaa ggcggcaacc tcggcgttaa
    15241 ccggtttgat ggtggtagga gggcgatgat ctaagtaaga ggcaatcacc ggtgccggta
    15301 agcccatcgt ttttgccagt aaagcgatgc tttgctcgcg ctggctgcgg gttaacgcat
    15361 cggcctcact aaaggttgcc agtacgccct gaataaaagc gccgtttttt tctgcatagg
    15421 gacgagctgc cagataaaac gatccagttt gattgagatc ggtgccgtct ttcagcaccc
    15481 gcacgccgcc ctgtaataat gcagcagagt agtagggatc ccagatagcc caggcgtcaa
    15541 cgttaccttg ctggaacgcg gcgcgggcat cagcgggcgt caggtaagtg ggttggatat
    15601 cggtaaactt aagtccggcc tgacgcagtg cacgcagtaa aaggttgtgt gaactggaac
    15661 ctttctgaaa ggcaactttg tgacctttaa gatcggctac ggttttgatc gggctgtttt
    15721 ctgccaccag aatcacttcg gctttgggct tcggtggctc gacgcccaca tataccaaat
    15781 cagccccggc agcctgggca aagattggcg gaatatcccc ggtactgccg agatcaatac
    15841 tgccaacgtt taaggcttcc aacatttgcg gacccgcggg gaactccacc caggagattt
    15901 ttgattgcgg atagcgtttt tccagtaact ggtggctttt tgccagtacc ataccaatac
    15961 tgcctttctg atagcctata cataacgctt caggcgagga ttctgcagca accgcaatcg
    16021 tagagacgct aagcaatccc accagcgcca gtttaatgat gttacgcatg ggcattacct
    16081 cgcagagaca gaaggtcagg aacctgaaca tcgcggcggt gcaatgcctg ccagaaagtt
    16141 tctagcgcgg tatcaagacg ggtttgcaga tttggcgtga actggggtct gtgatggtaa
    16201 tcaattactt gtgagtcatc ggcaaacacg ccgtgcagga tctcctgagc tttcagtgcg
    16261 cttaaaactg gtttaagggc ataatcgacc gccagcagat gggccacggt accgcccgtc
    16321 gccagcggta gcaccacttt gccttgcaaa gcgcgctctg gcagcaggtc gagcagggtt
    16381 ttcagcgcgc cggaataggc ggctttatac acaggtgtag cgactatcag tccatcagcc
    16441 tgttgcagct gttcggtgaa ggtcttgagt gccggactat cgaaacgagc ataaagcaga
    16501 tcttccggga cgaagttttg cagattccag tgataaacct ctacatccag gccatttagt
    16561 ttttcccgcg catattccag caaggagctg gagcgagaag gaaagcgagg actacccgcc
    16621 agggtgatga cacgcatact ctctccttat aaccaattgt tctttttttg ttaacattga
    16681 taacaattcg gtcagtctgt cggagagaca agaaaatgcc aaatataaat tttgtgtatc
    16741 tttttctgaa aataaactaa agaaagggct ttatatatca cgcatattta tttgttggat
    16801 agtcattaga tgttgaatca atgaattact cgttcagtta atatttaaat attcatgaaa
    16861 tctataaatt aaagatttgt cacttattgg atttagtatg cttgatttaa atgacggtca
    16921 attattgtta ttattaagtc taatgtcatt taaaggcatt atcatattta cgaagagatc
    16981 aggatgatga cgatgaaaaa aagtgtattg acggcgttta taactgtggt atgtgcaacg
    17041 tccagcgtta tggcagctga tgataatgct atcacggatg gcaaagtaac atttaatggt
    17101 aaagttattg ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg
    17161 ccaaatgtca gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat
    17221 gtaccaattg ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt
    17281 agtggtacgg ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca
    17341 gc
//
LOCUS       DAJVOY010000079        17282 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVOY010000079 DAJVOY010000000
VERSION     DAJVOY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.079"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..2440)
                     /gene="topA"
                     /locus_tag="OQL90_002300"
     CDS             complement(<1..2440)
                     /gene="topA"
                     /locus_tag="OQL90_002300"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1369056.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRY"
     gene            2820..3071
                     /locus_tag="OQL90_002301"
     CDS             2820..3071
                     /locus_tag="OQL90_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1369057.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3107..4156)
                     /gene="sohB"
                     /locus_tag="OQL90_002302"
     CDS             complement(3107..4156)
                     /gene="sohB"
                     /locus_tag="OQL90_002302"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1369058.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4376..5134
                     /locus_tag="OQL90_002303"
     CDS             4376..5134
                     /locus_tag="OQL90_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1369059.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5131..5722
                     /gene="cobO"
                     /locus_tag="OQL90_002304"
                     /pseudo
     CDS             5131..5722
                     /gene="cobO"
                     /locus_tag="OQL90_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5762..6634)
                     /gene="rluB"
                     /locus_tag="OQL90_002305"
     CDS             complement(5762..6634)
                     /gene="rluB"
                     /locus_tag="OQL90_002305"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1369060.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(6845..8740)
                     /locus_tag="OQL90_002306"
     CDS             complement(6845..8740)
                     /locus_tag="OQL90_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCS1369061.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8768..9388)
                     /locus_tag="OQL90_002307"
     CDS             complement(8768..9388)
                     /locus_tag="OQL90_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1369062.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9385..10266)
                     /gene="yciV"
                     /locus_tag="OQL90_002308"
     CDS             complement(9385..10266)
                     /gene="yciV"
                     /locus_tag="OQL90_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1369063.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10404..10448
                     /gene="trpL"
                     /locus_tag="OQL90_002309"
     CDS             10404..10448
                     /gene="trpL"
                     /locus_tag="OQL90_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1369064.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10425..10518
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10540..12102
                     /gene="trpE"
                     /locus_tag="OQL90_002310"
     CDS             10540..12102
                     /gene="trpE"
                     /locus_tag="OQL90_002310"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1369065.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12102..13697
                     /gene="trpD"
                     /locus_tag="OQL90_002311"
     CDS             12102..13697
                     /gene="trpD"
                     /locus_tag="OQL90_002311"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1369066.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13698..15059
                     /gene="trpCF"
                     /locus_tag="OQL90_002312"
     CDS             13698..15059
                     /gene="trpCF"
                     /locus_tag="OQL90_002312"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1369067.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFNPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15071..16264
                     /gene="trpB"
                     /locus_tag="OQL90_002313"
     CDS             15071..16264
                     /gene="trpB"
                     /locus_tag="OQL90_002313"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1369068.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16264..17070
                     /gene="trpA"
                     /locus_tag="OQL90_002314"
     CDS             16264..17070
                     /gene="trpA"
                     /locus_tag="OQL90_002314"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1369069.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4171 a         4622 c         4310 g         4179 t
ORIGIN      
        1 gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc accagtggcg
       61 cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc gcagcgccgt
      121 cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat ggcaccggat
      181 cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt ttacactctt
      241 cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt tcagagccac
      301 atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg ccctcttcga
      361 tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt ttcggatcga
      421 tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg cgcagcgcgt
      481 tggtttcagc gtcttcgcct tccagtacgt tcagcacttc gttttccggc accaggttaa
      541 tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca aggaataccc
      601 cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca atgctggtca
      661 gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct ttatctaact
      721 gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct tcgtgatttg
      781 ccacctggtc gaggctgttt tccatctgtg cggtgaagtc gtagttcatt aactcgcgga
      841 aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag aaacgacgat
      901 tttctactcg cacgtagcca cgatcctgaa tggtcgaaat gatcgacgca taggtagacg
      961 gacgaccgat accgcgtttt tccagctctt taaccagcga tgcttcactg aaacgggctg
     1021 gcggcttggt aaagtgctgg gctggtgtaa gttcaacgag cgtcagagca tcgcctttat
     1081 taactgctgg taagatgcga tcttcatcgc ctttacgcaa cgcaggcatc acttttgtcc
     1141 agccatcaaa acgcaaaata cgacctcgtg ctttcaggcg gaaatcgccc gcaccaacgg
     1201 tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac tgacgccaga
     1261 ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc gccatcacat
     1321 tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg ctggcgtact
     1381 gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa ccgcgaacca
     1441 tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg atatagcctg
     1501 cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca agacgggtgc
     1561 tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg gttgtcggtt
     1621 tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc gcagcctgag
     1681 tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc tgtaacgcca
     1741 acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct tccggcacga
     1801 acgctttaat ttcacgctca cgctcgacca ccaggcgaac cgccaccgac tgcacacgac
     1861 cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata taccccacca
     1921 cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc agctcacccg
     1981 gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc actcggctat
     2041 agcgcgcatc atcaccccca atcacttccc gcaggtgcca tgcaatggct tccccttcgc
     2101 ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt ttcagttcag
     2161 agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg tgccacgggt
     2221 caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt ttagccgtct
     2281 tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc aaatcgcgga
     2341 tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg atcgttttgg
     2401 cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta cctaatttga
     2461 ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt taccgatatg
     2521 gactcgttct ctgcggatat caaccccttg tcgtgactct gttcacgaaa attcaggtga
     2581 ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg gtcgaatgtc
     2641 aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa atccgatacg
     2701 caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc accagcgtgg
     2761 tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttatttaag gagtaaataa
     2821 tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc aacaaaatca
     2881 ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg cagcgtaatg
     2941 gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca acagccaaaa
     3001 gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa aaatatcatc
     3061 tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac atcaatggct
     3121 tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc gcgctgccgg
     3181 tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact tcacggcctt
     3241 ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg cctttctcta
     3301 ccgcctgttg tccgtaccag tgttcacctg ttgccacctg ttcaatatcc agagacggac
     3361 gcatacgctt cacaaaatct ttaaacaact gatgcgtttc gttcaactct tcgcggaatt
     3421 tctcccgccc ttcttcggta ttttcaccca gcaacgtcag agtacgctta tactgcccgg
     3481 cagtgtgcag ttcgatatca atatatttgc ttttcaggaa gcggttaaag ttgggcattt
     3541 gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt ttgtccgcca
     3601 cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca gttaaaggaa
     3661 tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca tgcaccatgc
     3721 caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct gcgagtaccg
     3781 ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct ttaaaatcca
     3841 gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct ttcgcttttg
     3901 ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt ttttgctgat
     3961 gtgagcccat cagcgccgcg gccagttctt ctttcatctc cttatactgt tcgctgagat
     4021 tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg acaataatgg
     4081 cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac aaaccatatt
     4141 cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc acagtttaca
     4201 acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga caaagtatcc
     4261 attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag gcataaagcc
     4321 ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag tagccatgca
     4381 ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg gagccagcga
     4441 tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag tgattctgtt
     4501 gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag aaactgggcg
     4561 tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt gccaacaact
     4621 ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata atgccggatt
     4681 gctcggcgat atttgcccaa tgagcgaaca aaatccgcag gtctggcagg acgtcatgca
     4741 ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat tactcaaatc
     4801 ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac gagctaactg
     4861 gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac tggccgatga
     4921 atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca ccgcaatgcg
     4981 cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg atatcatgcc
     5041 gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga cctttgacgc
     5101 ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca acagcgtcag
     5161 cagcgagtga aagaaaaagt agatgctcgt gtggcacagg ccccaggatg aacgcggtat
     5221 tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg gtacggcaac
     5281 acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag gcacctggcc
     5341 taatggcgaa cgcaatctgc tggaaccaca tggcgttgag tttcaggtga tggcaacggg
     5401 ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg aagtctggca
     5461 acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg atgaactgac
     5521 gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt taaatgaacg
     5581 tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata ttcttgaact
     5641 ggcggatacg gtaagtgagc tgcgccccat caaacatgcg tttgatgccg gtgttaaggc
     5701 gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg gcatactttg
     5761 attaaccgtt attacgcccg ccagaacgac gaccgccgct cacctgactg tgacgtttca
     5821 ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca gcgactttag
     5881 agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg gcgaggtcca
     5941 gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg taacgaacac
     6001 ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact tcacggttac
     6061 ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg ctgaacttga
     6121 tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa tcacgcagtt
     6181 tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact tcacggcttg
     6241 gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga ccacaggtgt
     6301 taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc agacggtcaa
     6361 acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc ggtttgtaat
     6421 aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga tgaccatcga
     6481 tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca attttgccat
     6541 caacactcac acgaccggct tcaataatag attcgatttc acggcgggag ccgtggccag
     6601 cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt gtcgccttcc
     6661 caggcgtctg atatgctttt gtgcaacagt gagtcagaac ttacatctga ctcactgata
     6721 gtaaagtctg tttgaactct tcaggcctta agtaaacgac tcgacgttta tcggcctggc
     6781 tttgaggcgc aacatactac actatcttta tggttaaagc ttgtttcgat taacatcagc
     6841 ttaattacca gccaccaccg cctccgccac ccgcgccgcc gccagcacca ccaccggaat
     6901 aacttcgccc caacaaactg gtattggccc cgcaacttag gtgcgtctgt aatgagaata
     6961 aaacgccgtt acgggcaatc tctggagcgc gaatcgccga agaaagcgaa ggggcaatgc
     7021 gtaacgccca ttccctcccc aaatttgccg atatagccca acctagcaac gcgtaatcca
     7081 gtttccgtgt ttcccctttc cgccgacgtc cactgtgtgt tgctgcctct ttacgcgcaa
     7141 gatagttaac gatagcttca ccgcgggcat aacgttgctg cccagtttgg gtaaactttg
     7201 gcagaaaaat ataccccatg ccagtgagat aaccggtcag gcaaacagcg ctaaaataac
     7261 ccgctggcat ataccagtaa aagaatacat ctccgacatt gatatagaga gaatagaagg
     7321 ccacgcctga acaaatcagt ggtaaaaaca aagtgatgat tgcacgatta acgcaaggct
     7381 tgtcatcatc gcgggcaaac aaaagctcaa gggaacaaaa taaaaaagga ataatccagc
     7441 agatcataat aaacatatcg cctaccagac tcattcctgc ccaacctacg ggactcaggt
     7501 taccgtagag aatgatcgcc aatattgcca gtccacccca ttgcaaaaac gtatcaggcc
     7561 ggtaaaacaa agacttttta cgcggcagat aaaatttctg catccgtaaa aatgccctgc
     7621 gtaagactct gttgcgtctc cccgtaagaa cgaccttatc gcctttacga aataacgctt
     7681 ccagtaataa ctgctctgcg ggcagtaata cctcattatt tctggtcgcg ccttcattaa
     7741 cacaaatcag gatttgctgg ttcttatcat actgatcttc cagatgaatt tttcccttca
     7801 cgatcagatt tacgatatcg gcacagaaac ctttatcgtc gtaaaccagc ttcgcatcga
     7861 gacgtaacat gccgggcgtg taatctggcg gaatgacatc ggtttcaatc acatctaccg
     7921 gcgtaaattg cggcctgcgc ttccagagat ataaccatcc acaaaccagg aatagacacg
     7981 gaaaccaaat taacaacgat gacgtggagg gtaaaagaag atggctgaaa atattcgtcg
     8041 tttgtggagc cggggcattg ctaagtaacg cgtgaggcca gcggtagagt accgtgaaat
     8101 cttcacgata aaacggatcg cgggattcta ttcttccgtc ctcaaggatg cggccatttc
     8161 gatatgtgtc gccctcttct ccagtaaaga gatcgatttc gctaaatgga ttacccgcga
     8221 tatcagggaa cttgagtgaa aatcgggtct tatagatttc aaacggccag tggttaccag
     8281 tcacgttcca gattaacaga tctgaatctc cctcacgcag gaaagcattt ttaacctgat
     8341 aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg cccacgacaa
     8401 tactcattaa cccgatatgc cagtcaagat gccagggttc aggaatacca tcacgagtga
     8461 cgccaacaat atgatagttc agcagtgcag cgtccccatc aggctgcatc cagagtcgtg
     8521 gtaaatcacg ataaaatccg tgcttaatct gctgatttcg cgcctgaaaa tgaatatttt
     8581 ctttgatgtc gagcacccca tccggttgca gagtggcttc aacatcaaag agcgagatcc
     8641 cttcttcata agaagggatc tccgggtttt ctgcataact tagagatgat acaaacagcc
     8701 ctgctatcaa cagcaaaatg cagcgaaact tccctgccat agtcttccat ccgtggaacg
     8761 caatttgtca taagaaaggc ttcacatcac ccacgccttc acgcaccacg accggcgtat
     8821 catcggtaag atcaatgacc gttgtcggtt tctgtccgag atagccgccg tggataatca
     8881 aatccacctg tttttccaga cggtctttaa tttcttctgg atcggattcg gtaaattcgc
     8941 tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca agcaacgcct
     9001 gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc tgcaacagac
     9061 ggcgtggcac ctccttcgtc cccttcagga tgaaggtata gttgcccggc gtgttgtttt
     9121 tcattaaacg aaacgcaacg ttatccacga atgaataggt cgaaagttca gaaagatcgc
     9181 gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa atacgctcca
     9241 tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt ggataaacaa
     9301 tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc tgcgggttat
     9361 caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct gcggctgttc
     9421 ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac ccagttcgat
     9481 ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat gctgacgcgc
     9541 aagtgtcgtc aactgggtgc gttcattggg cgattgctgg cactgcgcga cttccatcgc
     9601 gtcgccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag cactaagatt
     9661 gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga catcaatagc
     9721 ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg ccagatactt
     9781 tttaaagaca tctgccattg aactggcttt gccgcactca acaaggaaac gcgcaaaatg
     9841 accgcgcgtc actgcgccgc cctgcgccag tcgttgtgcg ccttccagcg ccccaggtat
     9901 ttgcgctttt tcaagtcttt cggcaatttg ctgcgcccgc tgattgcgcc gttctgtctg
     9961 ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc cgacaatatg
    10021 aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat tcagcgccag
    10081 tcccgaacgt gaaatttcct ctcttgctgg cgcgattgca gctgtggtgt catggtcggt
    10141 gatcgccagg gtgccgacgc gcatctcgac tgcacggtgc accaatgctt ctggcgtcag
    10201 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt aattcgtgtc
    10261 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
    10321 caaatattct gaaatcagct gttgacaatt aatcatcgaa ctagctaact agtacgcaag
    10381 ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct tggtggcgca
    10441 cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc agcccgccta
    10501 atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca aaaaccgact
    10561 ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct ttttcaccag
    10621 ttgtgtgggg atcgtccggc aacgctgctg ctggaatccg cagatatcga cagcaaagat
    10681 gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt aggtgacact
    10741 gtcacaattc aggcactttc cggcaacggc gaagccctgc tgacactact ggataacgcc
    10801 ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg cttcccccct
    10861 gtcagtccac tgctggatga agacgctcgc ttatgctccc tttcggtttt tgacgctttc
    10921 cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat gttcttcggc
    10981 ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact gtcagcggaa
    11041 aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat tgaccatcag
    11101 aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga aaaacaacgt
    11161 ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc gccgctgccg
    11221 gtggtttccg tgccgcatat gcgttgtgaa tgtaaccaga gcgatgaaga gttcggtggc
    11281 gtggtgcgtt tgttgcaaaa agcgattcgc accggagaaa ttttccaggt ggtgccgtct
    11341 cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct gaaaaagagt
    11401 aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt tggcgcgtcg
    11461 ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta cccgattgcc
    11521 ggaacacgcc cgcgcggtcg tcgcgccgat ggttcactgg acagagacct cgacagccgc
    11581 atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat gctggtggat
    11641 ctcgcccgta atgatctggc acgcatttgc acccccggca gccgctacgt cgccgatctt
    11701 accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt cggtgagctg
    11761 cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg gacgttaagc
    11821 ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg tcgccgcggc
    11881 agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac ctgcattgtg
    11941 atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc tggcatagtc
    12001 cttgattctg ttccgcagtc ggaagccgac gaaacccgta ataaagcccg cgctgtactg
    12061 cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac attctgctgc
    12121 tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc aatgggcata
    12181 atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc ctggcgacga
    12241 tgagcaatcc ggtgctgatg ctttctcctg gccccggtgt gccgagcgaa gccggttgta
    12301 tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt tgcctcggac
    12361 atcaggcgat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa attctccacg
    12421 gtaaagcctc cagcattgaa cacgacggtc aggcgatgtt tgccggatta acaaacccgc
    12481 tgccagtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt ttaaccatca
    12541 acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc gtttgtggat
    12601 tccagttcca tccggaatcc attcttacta cccagggcgc tcgcctgctg gaacaaacgc
    12661 tggcctgggc gcagcggaaa ctagagccaa ccaacacgct gcaaccgatt ctggaaaaac
    12721 tgtatcaggc gcagacgctt agccagcaag aaagccacca gctgttttca gcagtggtac
    12781 gtggcgaact gaaaccggaa caactggcgg cggcgctggt gagcatgaaa attcgcggcg
    12841 agcacccgaa cgagatcgcc ggagcagcaa ccgcgttact ggaaaacgcc gcgccgttcc
    12901 cgcgcccgga ttatctgttt gccgatatcg tcggcaccgg tggtgacggc agtaacagca
    12961 tcaatatttc caccgccagt gcgtttgtcg ccgcggcctg tgggctgaaa gtggcgaaac
    13021 acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg gcgttcggta
    13081 ttaatcttga tatgaatgcc gataaatcgc gccaagcgct ggatgagtta ggcgtatgtt
    13141 tcctctttgc gccgaaatat cacaccggat ttcgccatgc gatgccggtt cgccagcaac
    13201 tgaaaacccg cactctgttc aacgtgctgg gaccattgat taacccggcg catccgccgc
    13261 tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa accttgcgcg
    13321 tgctggggta tcaacgcgcg gcggtagtgc acagcggcgg tatggatgaa gtttcattac
    13381 acgcgccgac aatcgttgcc gaactgcatg acggtgaaat taagagctat cagctaactg
    13441 cagaagattt tggcctgaca ccctaccacc aggagcaact ggcaggcgga acaccggaag
    13501 aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgct cacgaagcag
    13561 ccgtcgcggc gaatgtcgcc atgttaatgc gcctgcatgg ccatgaagat ctgcaagcca
    13621 atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga gttaccgcac
    13681 tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca gacaaggcga
    13741 tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat gaggttcagc
    13801 cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt attctggagt
    13861 gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcaatccg gcacgcattg
    13921 ccgccattta taaacattac gcttcggcaa tttcggtgct gactgatgag aaatattttc
    13981 aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg attttatgta
    14041 aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag gccgatgcct
    14101 gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca gccgtcgctc
    14161 acagtctgga gatggggggg ctgaccgaag tcagtaatga agaggaactg gagcgcgcca
    14221 ttgcattagg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat ttgtcgattg
    14281 atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtaacg gtaatcagcg
    14341 aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct aacggttttc
    14401 tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc cgggtgttgc
    14461 tgggtgagaa taaagtatgt ggcctgacgc gtgggcaaga tgctaaagca gcttatgacg
    14521 cgggcgcgat ttacggtggg ttgatttttg tcgcgacatc accgcgttgc gtcaacgttg
    14581 aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg ttccgcaatc
    14641 acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca gtgcaactgc
    14701 atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca acacatgttg
    14761 ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagttt cagcacgttg
    14821 ataaatatgt tttagacaac ggccagggtg gaagcgggca acgttttgac tggtcactat
    14881 taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca gataactgcg
    14941 tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta gagtcgcaac
    15001 cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc gcatattaag
    15061 gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg gcatgtacgt
    15121 gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca gcgcgcaaaa
    15181 agatcctgaa tttcaggctc agttcaacga cctgctgaaa aactatgccg ggcgtccaac
    15241 cgcgctgacc aaatgccaga acattacggc tgggacgaac accacgctgt atctgaagcg
    15301 cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc aggctttact
    15361 ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc agcatggcgt
    15421 ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata tgggtgccaa
    15481 agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg cggaagtgat
    15541 cccggtacat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc tgcgcgactg
    15601 gtccggcagt tatgaaaccg cgcactatat gctgggcacc gcagctggcc cacatcctta
    15661 tccgaccatt gtgtgtgagt ttcagcggat gattggcgaa gaaacgaaag cgcagattct
    15721 ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg gttcgaatgc
    15781 catcggcatg tttgcagatt tcatcaacga aaccaacgtc ggcctgattg gtgtggagcc
    15841 gggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg gtcgcgtggg
    15901 catttatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga ttgaagaatc
    15961 ttactccatc tccgccggac tggatttccc gtctgtcggc ccacaacacg cgtatcttaa
    16021 cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg aagccttcaa
    16081 aacgctgtgc ctgcacgaag ggattatccc ggcgctggag tcttcccacg ccctggccca
    16141 tgcgctgaaa atgatgcgcg aaaccccgga aaaagagcag ctactggtgg ttaacctttc
    16201 cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac gaggggaaat
    16261 ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga aggcgcattc
    16321 gttcctttcg tcacgctcag ggatccgggc attgagcagt cattgaaaat tatcgatacg
    16381 ctaattgaag ccggtgctga cgcgctggag ttaggtatcc ccttctccga cccactggcg
    16441 gatggcccga ccattcaaaa cgccactctg cgcgcctttg cggcaggtgt gactccggca
    16501 caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc cattggcctg
    16561 ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc ccagtgcgag
    16621 aaagtcggcg tcgattcggt gctggttgcc gatgtgccag ttgaagagtc cgcgcccttc
    16681 cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc aaatgccgat
    16741 gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt gctgtcacga
    16801 gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct ggttgcgaag
    16861 ctgaaagagt acaacgctgc acctccattg caggggtttg gtatttccgc cccggatcag
    16921 gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt ctggttcggc cattgttaaa
    16981 atcatcgagc aacatattaa tgagccagag aaaatgctgg cggcactgaa agtttttgta
    17041 caaccgatga aagcggcgac gcgcagttaa tcccacagcc gccagttccg ttggcggcat
    17101 tttaactttc tttaatgaag ccggaaaaat cctaaattca tttaatattt atctttttac
    17161 cgcttcgctt acccctgtcg aacgtcaact tacgtcattt ttccgcccaa cagtaatata
    17221 atcaaacaaa ttaatcccgc aacataacac cagtaaaatc aataattttc tctaagtcac
    17281 tt
//
LOCUS       DAJVOY010000080        17235 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVOY010000080 DAJVOY010000000
VERSION     DAJVOY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.080"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            48..443
                     /locus_tag="OQL90_002315"
     CDS             48..443
                     /locus_tag="OQL90_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1369070.1"
                     /translation="MRWEDIRDGRLYVEQQKTGAKIRISLPTTISRLNLTLTDVLDNL
                     KKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKGS
                     DFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            679..1497
                     /gene="ybhA"
                     /locus_tag="OQL90_002316"
     CDS             679..1497
                     /gene="ybhA"
                     /locus_tag="OQL90_002316"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCS1369071.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1498..2556)
                     /gene="modC"
                     /locus_tag="OQL90_002317"
     CDS             complement(1498..2556)
                     /gene="modC"
                     /locus_tag="OQL90_002317"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1369072.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2559..3248)
                     /gene="modB"
                     /locus_tag="OQL90_002318"
     CDS             complement(2559..3248)
                     /gene="modB"
                     /locus_tag="OQL90_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1369073.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3248..4021)
                     /gene="modA"
                     /locus_tag="OQL90_002319"
     CDS             complement(3248..4021)
                     /gene="modA"
                     /locus_tag="OQL90_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1369074.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4187..4336)
                     /gene="acrZ"
                     /locus_tag="OQL90_002320"
     CDS             complement(4187..4336)
                     /gene="acrZ"
                     /locus_tag="OQL90_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1369075.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4465..5253
                     /gene="modE"
                     /locus_tag="OQL90_002321"
     CDS             4465..5253
                     /gene="modE"
                     /locus_tag="OQL90_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1369076.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5321..6793
                     /gene="modF"
                     /locus_tag="OQL90_002322"
     CDS             5321..6793
                     /gene="modF"
                     /locus_tag="OQL90_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1369077.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7054..8070
                     /gene="galE"
                     /locus_tag="OQL90_002323"
     CDS             7054..8070
                     /gene="galE"
                     /locus_tag="OQL90_002323"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1369078.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8080..9126
                     /gene="galT"
                     /locus_tag="OQL90_002324"
     CDS             8080..9126
                     /gene="galT"
                     /locus_tag="OQL90_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1369079.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9130..10278
                     /gene="galK"
                     /locus_tag="OQL90_002325"
     CDS             9130..10278
                     /gene="galK"
                     /locus_tag="OQL90_002325"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1369080.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10272..11312
                     /gene="galM"
                     /locus_tag="OQL90_002326"
     CDS             10272..11312
                     /gene="galM"
                     /locus_tag="OQL90_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1369081.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11514..12266
                     /gene="gpmA"
                     /locus_tag="OQL90_002327"
     CDS             11514..12266
                     /gene="gpmA"
                     /locus_tag="OQL90_002327"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1369082.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12424..13476)
                     /gene="aroG"
                     /locus_tag="OQL90_002328"
     CDS             complement(12424..13476)
                     /gene="aroG"
                     /locus_tag="OQL90_002328"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1369083.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13792..14172
                     /locus_tag="OQL90_002329"
     CDS             13792..14172
                     /locus_tag="OQL90_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1369084.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14286..15227
                     /gene="zitB"
                     /locus_tag="OQL90_002330"
     CDS             14286..15227
                     /gene="zitB"
                     /locus_tag="OQL90_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1369085.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15224..15943)
                     /gene="pnuC"
                     /locus_tag="OQL90_002331"
     CDS             complement(15224..15943)
                     /gene="pnuC"
                     /locus_tag="OQL90_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1369086.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(15981..17024)
                     /gene="nadA"
                     /locus_tag="OQL90_002332"
     CDS             complement(15981..17024)
                     /gene="nadA"
                     /locus_tag="OQL90_002332"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1369087.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4366 a         4495 c         4311 g         4063 t
ORIGIN      
        1 tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg agatgggagg
       61 atataagaga tggcagacta tacgtggaac aacaaaaaac aggggcaaaa attcgcattt
      121 ctctccccac cacgatttcc agattgaatt taacacttac cgatgtactg gataacttga
      181 aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg acaatcgcag
      241 cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac ccaccgccat
      301 ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc agtgatttca
      361 ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt gacggacgag
      421 ggagggaatg gaaagacatt tagaattaat ttcgaatgat ttcgaaacac cacttgtaat
      481 taattgatta taaaggatta attcctaaga ggcatttgct tcatgaatgc tcctttgcat
      541 ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc tgaattaaaa
      601 cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa aattcttaac
      661 cttaatctgg aatacgccat gaccacacgc gtgattgctc tcgacttaga cggcacctta
      721 ttgaccccga aaaagaccct gcttccttca tcgatagaag ccctggcccg cgctcgcgaa
      781 gcaggctatc aattaatcat cgtcacaggt cgccatcacg tcgctattca tcctttttat
      841 caggcgctgg cgctggatac acctgctatt tgctgtaatg gcacctattt gtatgattat
      901 catgcaaaaa ccgtgctgga agcggaccca atgcccgtta ataaagccct gcaactcatt
      961 gagatgctga atgaacacca cattcacggt ctgatgtatg tggatgatgc aatggtctat
     1021 gagcacccga ccgggcatgt cattcgcacg tctaactggg cgcaaaccct gccgccggaa
     1081 cagcgtccga ctttcacaca agtcgcttct ctggctgaaa cggcgcaaca agttaacgcc
     1141 gtatggaagt tcgccctgac gcacgatgac atgccgcaat tgcagcattt tggtaagcat
     1201 gtcgaacatg aactgggact ggagtgtgaa tggtcctggc acgatcaggt tgatattgca
     1261 cgcggcggca acagcaaagg taaacgtttg acgaaatggg ttgaggcgca aggctggtcg
     1321 atggaaaacg tcgtggcgtt cggcgataac tttaatgata tcagtatgct ggaggccgct
     1381 ggtacaggcg tggcgatggg caacgccgat gacgcggtaa aagcgcgcgc caacattgtg
     1441 attggtgata acaccaccga cagcattgcc cagttcattt atagccacct gatttaatca
     1501 ggcggttatc gacacacttt taatttgcgc gtacagccac aggcctggtt tgatcgccag
     1561 ttcatccctg gcccacgggc tgatacgcgc ccacagcgtt ttaccgccga cttccagctc
     1621 cacttccacc tgaccgttgt cgtcataact attaacaact tttgcccgca atacgttacg
     1681 aatgctggtt tgctgcggcg gttgtaaaac cagagaaaca tcggaagcct gaatgcggat
     1741 acgtagcgca gcttgcagcg gttcgtccag cttattgacc cacaaatgct gatcgcccag
     1801 cgccagcgcg gtcatcgcgt aatgcggatg atgctcaagc accgtcactt tcagaatgct
     1861 actttgttgc tctttcggca gccacggatt catcacgcta ctaccccaga cttcttccag
     1921 tgcgccaaag gctttcacct gaccgttttc cagtaccatc actctgtctg ccagatggag
     1981 gatctcatcc agcgaatggc tgacatacaa catcgggatg ttgatttccc gcgtcagacg
     2041 ttgcagataa ggcaacagtt cgcgtttacg cggaatatcc agtgacgcca gcggttcatc
     2101 cagcaacagc aattctggtg ccgtcagcaa agcccgccca atcgctacgc gctgtttttc
     2161 gcctccggac aggctgcctg gtaaacggtc aagcaacggt tcaatgccta aaagcgccac
     2221 cagcttatcg aactgatcga ccatgctttt cgccatgccg tagcgcagat tgccacgcac
     2281 tttgtaatgc ggaaacagcc gcgcatcctg aaaaacatag ccaacgcgac gcttttctgg
     2341 cgtcaggcag ataccttttt cggcatcatt tagtacccga ccattgagga caatcctccc
     2401 tttttgcggg cgcgtcagtc cactgatggc gttaatcagc gaagtttttc cggcaccgga
     2461 gacgccaaag atagcagtga tgccattggc gggcagcgtt tcattaatag tcaggcaatg
     2521 gttgcccaac gtctgggaaa aattcagttc cagcatgatt agcgccccgc ccgttcacgg
     2581 ctgattctgg ccagccattc tgaaatcaac agggagatca tcgccagcgc aatagaaata
     2641 atgcacagtc tcgccgcgcc actttccccg ccgggggtct ggatcagggt atacatggca
     2701 gagggaatgg ttcgcgtttc gccaggaatg ttcgacacaa aggtgatggt tgcaccaaac
     2761 tcaccgagag aacgagcaaa agccagtacc gtaccaacaa taattcccgg taaggtcagc
     2821 ggtaacgtga tagtaaagaa aacgcgccag cgcccggcac ctagtgttct tgcggcctgt
     2881 tcgagtttga catcaacccc ttccagcgcc agacgaattg cccgcaccat cagcggaaac
     2941 gacatgacgg cggcagcgag aaccgcgccg cgccagctaa aggcgaaggt aataccaaac
     3001 cagtcataca gacgttcacc gataaatccg cgccgtccca tcgaaactaa taataagtaa
     3061 ccgacgacca cgggcggtaa caccagcggt agatgcagta cgctgtcgag cagagctttg
     3121 cccggaaacg tgcaacgcac cagtaaccag gcaaaaaaga tcccaaacgg caggctaaac
     3181 agcacagcca gggaagaaac tttcaggctt aataaaactg cctgccattc tggatcggtc
     3241 agtatcatta cttggttgta aatccgtaac gtttaaagat ttccgctgcc tgcggtccct
     3301 tcagataatc ataaaatgct ttcactgtcg cattgttatg cccttccaca acagcaaccg
     3361 gatattccac ttttttatgt gaatcttccg ggaaggtggc aaccactttt acccctttgc
     3421 tgacaactgc gtcagaaccg taaacaatgc ccaacggcgc ttcattgcgt tcgaccaacg
     3481 ccagcgcgcc acgaacatct tccgccggag ccaatttcgg ggagagcgta tcccatgcgc
     3541 ccagtttttg cagtgcttct tttgcataaa tgccagcagg gacatgttcc ggatcgccaa
     3601 ccgcgaggcg accgccattc agcagcgaag tccagttagt tgtgctgtcg atgatgaaat
     3661 ctttctgctc gctggctttc ggtgctacaa cgaccaggct attgccgagc agtgtctggc
     3721 gcgaagccgt atcgatcgct tttttatcaa ccgcataatc catccatttc tgatcggcag
     3781 agataaacag atccgcaggc gctcccgctt caatctgacg ggccagagta gatgacgaag
     3841 cgaaagaaga aaccacatcc acgccttttt cttttttata ctgcgtagca atgtcctgca
     3901 ttgcgttagt cagtgatgcg gcggcgaaca ccgtgatttt cccttcatct gccagtgtat
     3961 tgccagcaac agcgaaagag agtgccgccc cggcaaacaa gttcaaccat ttacgagcca
     4021 ttggtaaccc cttaatgtaa cgttatatag gtgacaatat aacggctaac tcagaatttt
     4081 cccagcagtt attgctaacc tttgtgaggt agataagaaa aaatatcggc tggaaaagca
     4141 ggaagttgag aaaaagaaat gcccgactaa gtgggcattc agggaatcaa tgattttgtc
     4201 cgggctggtc ttttttacca acaccagaaa agatgttgaa tacttcacca agaccgtaaa
     4261 tcagacccag gatgatggcc atcacgacag gtaccatgat tacggcgaat accagacttt
     4321 ttaataactc taacatggtc aactccagat atagtcatga gactattcta accgctaagc
     4381 acagaaaagc actccccttt tgtgcggtca gctttgcgtg gcgttcgttt tccgtcacaa
     4441 taagactttt gccaggacat tgttatgcag gccgaaatcc ttctcaccct taagctccaa
     4501 caaaaattat tcgccgaccc gcgccggatt tcgctactaa aacacattgc gctttccggt
     4561 tccattagcc agggagcgaa agatgccggt atcagctata aaagcgcctg ggatgccatt
     4621 aacgagatga atcagttaag cgagcatatt ctggttgagc gcgcaacagg cggtaaaggt
     4681 ggcggcggcg cagtactgac ccgctatggt cagcgactga ttcagctcta tgacttactg
     4741 gcgcaaatcc agcaaaaagc ctttgatgtg ttaagtgacg atgacgccct gccgctgaac
     4801 agcctgctgg ccgcgatctc acgtttttca ctgcaaacca gcgcccgtaa ccagtggttc
     4861 ggtaccatca ccgcccgcga tcatgatgac gttcaacagc atgttgatgt cttactggct
     4921 gacggaaaaa cacgcctgaa agtcgcaatt accgcacaaa gcggcgcacg tctggggctg
     4981 gatgaaggca aagaagtgtt gatattgcta aaagcgccgt gggtaggcat tactcaggac
     5041 gaagcggtcg cacaaaacgc caacaaccaa ttaccgggta ttattagtca tattgagcgc
     5101 ggcgcagagc agtgcgaagt attaatggcg ctacccgacg ggcaaacact gtgcgccaca
     5161 gtgccggtaa atgaagcgac ttctcttcag caagggcaga atgtcacggc ctactttaat
     5221 gccgacagcg tgattatcgc cacgctgtgc taagcgtgtt gacaatttgt tatgaaacac
     5281 gtatccctgt cagtaatcgc tgcacaaaat gggatataaa atgtcatcgt tgcaaatttt
     5341 gcaaggcacg tttcgtctta gcgacacaaa aacgctgcaa ttgcctcagc taacgttaaa
     5401 cgcgggtgat agttgggcgt ttgtcggttc gaatggaagc gggaaatcgg ccctggcccg
     5461 cgcgctggcg ggggaacttc cgcttttgaa aggtgaacgg caaagccagt tttcccacat
     5521 cactcgtctc tccttcgagc aattgcaaaa actcgtcagc gacgaatggc agcggaataa
     5581 caccgatatg ctcggccctg gcgaagatga caccggacgc actacggctg agatcattca
     5641 ggatgaagta aaggatgcac cgcgttgcat gcaactggcg cagcagttcg gtattaccgc
     5701 cctcctcgac cgacgcttta aatacctttc cactggcgag acgcgaaaaa tcctgctgtg
     5761 tcaggcgctg atgtcggagc ctgacttgtt gattcttgat gagccgttcg atggcctgga
     5821 tgttgcctca cgtcagcagc tggctgagcg actcgcctcg ttacatcagt ccggtattac
     5881 tctggtactg gtgctcaatc gcttcgatga gatcccggag tttgtccagt ttgctggcgt
     5941 gctggcggat tgcacgttag cggaaactgg cgctaaagag gaactgctcc aacaagcact
     6001 cgtcgcgcaa ctggcacata gtgaacagct tgaaggtgtg caactgccgg agccggatga
     6061 accttcagca cgtcacgcct tacccgccaa cgaaccgcgc attgtgctga acaatggcgt
     6121 ggtttcttat aacgatcgcc ccattcttaa taaccttagc tggcaggtga atccaggtga
     6181 acactggcaa attgtcgggc caaatggtgc aggaaaatcg acgttattaa gcctggttac
     6241 tggcgatcat ccgcaaggtt acagcaacga tttgacgctt ttcggacgac gtcgcggcag
     6301 cggcgaaacc atctgggata tcaaaaagca tatcggttac gtcagcagta gtttgcatct
     6361 ggattaccgg gtcagcacta ccgtgcgtaa tgtgattctt tctggctatt ttgattcgat
     6421 tggcatttat caggccgttt cggatcgcca gcaaaaactg gtgcagcagt ggctggatat
     6481 tctcggcatt gataaacgta cggctgacgc tccgttccat agtctttcct ggggacagca
     6541 gcatctggcg ctgatcgtcc gcgcactggt gaaacatccg acgttgctta ttctcgatga
     6601 accactacag gggcttgatc cgctcaatcg ccagcttatc cgccgttttg ttgatgtgct
     6661 gattagcgaa ggtgaaacgc aattgttgtt tgtttcgcac cacgctgaag atgcgcctgc
     6721 ctgtattacc catcgccttg agttcgtgcc ggacggtgga ctctatcgct atgtgctgac
     6781 aaaaatatat tgagtcggta gtgctgacct tgccggaggc ggcctaagcg ccctctccgg
     6841 ccaacggttc gacgcatgca ggcatgaaac cgcttctttt ttcagataaa aagcgcaatc
     6901 attcataaac cctctgtttt ataatcactt aatcgcgcat aaaaaacggc taaattcttg
     6961 tgtaaacgat tccactaatt tattccatgt cacacttttc gcatctttgt tatgctatgg
     7021 ttatttcata ccataagcct aatggagcga attatgagag ttctgcttac cggtggtagc
     7081 ggttacattg gaagtcatac ctgcgtgcaa ttactgcaaa acggtcatga tgtcatcatt
     7141 cttgataacc tctgtaacag taagcgcagc gtactgcctg ttatcgagcg tttaggcggc
     7201 aaacatccaa cgtttgttga aggcgatatt cgtaacgaag cgttgatgac cgagatcctg
     7261 cacgatcacg ctatcgacac cgtgatccac ttcgccgggc tgaaagccgt tggcgaatcg
     7321 gtacaaaaac cgctggaata ttacgacaac aatgtcaacg gtactctgcg cctgattagc
     7381 gccatgcgcg ccgctaacgt caaaaacttt atttttagct cctccgccac cgtttatggc
     7441 gatcagccca aaattccata cgttgaaagc ttcccgaccg gcacaccgca aagcccttac
     7501 ggcaaaagca agctgatggt ggaacagatc ctcaccgatc tgcaaaaagc ccagccggac
     7561 tggagcattg ccctgctgcg ctacttcaac ccggttggcg cgcatccgtc gggcgatatg
     7621 ggcgaagatc cgcaaggcat tccgaataac ctgatgccat acatcgccca ggttgctgta
     7681 ggccgtcgcg actcgctggc gatttttggt aacgattatc cgaccgaaga tggtactggc
     7741 gtacgcgatt acatccacgt aatggatctg gcggacggtc acgtcgtggc gatggaaaaa
     7801 ctggcgaaca agccaggcgt acacatctac aacctcggcg ctggcatagg cagcagcgtg
     7861 ctggacgtgg ttaatgcctt cagcaaagcc tgcggcaaac cggttaacta tcattttgca
     7921 ccgcgtcgcg agggcgacct tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa
     7981 ctgaactggc gcgtaacgcg cacactcgat gaaatggcgc aggacacctg gcactggcag
     8041 tcacgccatc cacagggata tcccgattaa ggaacgacca tgacgcaatt taatcccgtt
     8101 gatcatccac atcgccgcta caacccgctc accgggcaat ggattctggt ttcaccgcac
     8161 cgcgctaagc gcccctggca gggggcgcag gaaacgccag ccaaacaggt gttacctgcg
     8221 cacgatccag attgcttcct ctgcgcaggt aatgtgcggg tgacaggcga taaaaaccac
     8281 gattacaccg ggacttacgt tttcactaat gactttgcgg ccttgatgtc tgacacgcca
     8341 gatgcgccag aaagcaacga tcctttgatg cgttgccaga gcgcgcgcgg taccagccgg
     8401 gtgatctgct tttcaccgga tcacagtaaa acgttgccag aactgagcgt tgcggcattg
     8461 acggaaatcg tcaaaacctg gcaggagcaa accgcagagc tgggaaaaac gtacccgtgg
     8521 gtgcaggtct ttgaaaacaa aggtgcggcg atgggctgct ctaacccgca tccgcacgga
     8581 cagatttggg caaatagctt cctgcctaac gaagctgagc gcgaagaccg cctgcaaaaa
     8641 gaatattttg ccgaacagaa atcaccaatg ctggtggatt atgttcagcg cgagctggca
     8701 gacggtagcc gtaccgttgt cgaaaccgaa cactggttag ccgttgtgcc ttactgggct
     8761 gcctggccgt tcgaaacgct actgctgccc aaagcccacg ttttgcggat caccgatttg
     8821 accgacgccc agcgcagcga tttggcgctg gcgttgaaaa agctgaccag tcgttatgac
     8881 aacctcttcc agtgctcctt cccctactct atgggctggc acggcgcacc atttaatggc
     8941 gaagagaatc aacactggca gctgcacgcg cacttttatc cgcctctgct gcgctccgcc
     9001 accgtacgta aatttatggt tggttatgaa atgctggcag agacccagcg agacctgacc
     9061 gcagaacagg cagcagagcg tttgcgcgca gtcagcgata tccattttcg cgaatccgga
     9121 gtgtaagaaa tgagtctgaa agaaaaaaca caatctctgt ttgccaacgc atttggctac
     9181 cctgccactc acaccattca ggcgcctggc cgcgtgaatt tgattggtga acacacggac
     9241 tacaacgacg gtttcgttct gccctgcgcg attgattatc aaaccgtgat cagctgtgca
     9301 ccacgcgatg accgtaaagt tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag
     9361 ttttccctca atgcgcccat tgtcgcgcat gaaaactatc aatgggcgaa ctacgttcgt
     9421 ggcgtggtga aacatctgca actgcgtaac aacagcttcg gcggtgtgga catggtgatc
     9481 agcggcaatg tgccgcaggg tgccgggtta agttcttccg cttcactgga agtcgcggtc
     9541 ggaaccgtat tgcagcagct ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac
     9601 ggtcaggaag cagaaaacca gtttgttggc tgtaactgcg ggatcatgga tcagctaatt
     9661 tccgcactcg gcaagaaaga tcatgccttg ctgattgact gtcgttcact ggggaccaaa
     9721 gcagtttcca tgccgaaagg tgtggctgtc gtcatcatca acagtaactt caaacgtacc
     9781 ctggttggca gcgaatacaa cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc
     9841 cagcagccag ccctgcgcga tgtcaccatt gaagagttca acgctgttgc acatgagctg
     9901 gacccaatcg tggcgaaacg cgtgcggcat atcctgactg aaaacgcccg caccgttgaa
     9961 gctgccagcg cgctggagca gggcgacctg aaacgtatgg gcgagttgat ggcggagtct
    10021 catgcctcta tgcgcgatga tttcgaaatc accgtgccgc aaattgacac tctggtagaa
    10081 atcgtcaaag ctgtgattgg cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc
    10141 ggctgtatcg tcgcgttgat cccggaagag ctggtgcctg ccgtacagca agctgtcgct
    10201 gaacaatatg aagcaaaaac aggtattaaa gagacttttt acgtttgtaa accatcacaa
    10261 ggagcaggac agtgctgaac gaaactcccg cactggcacc cgatggtcag ccgtaccgac
    10321 tgttaacttt gcgtaacaac gcagggatgg tagtcacgct gatggactgg ggtgcgactt
    10381 tactttcagc ccgtattccg ctttccgatg gcagcgtccg cgaggcgctg ctcggctgtg
    10441 ccagcccgga atgctatcag gatcaggccg cgtttctggg ggcctctatt ggtcgttatg
    10501 ccaaccgcat cgccaatagc cgttatacct ttgacggtga aaccgtgacc ctttcgccaa
    10561 gtcagggcgt taaccagctg cacggcggcc cggaagggtt cgacaaacgt cgctggcaga
    10621 ttgtgaacca gaacgatcgt caggtgctgt ttgccctgag ttcagatgat ggcgatcagg
    10681 gcttcccggg taatctcggc gcaacggtgc aatatcgtct gaccgacgat aaccgtatct
    10741 ccattactta tcgcgccaca gttgataaac cttgcccggt gaatatgact aatcacgtct
    10801 atttcaatct tgacggcgag cagtctgacg tgcgcaatca caagttgcag attctggcgg
    10861 acgaatatct gccggttgat gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg
    10921 gaacgtcttt tgatttccgc agcgccaaaa tcatcgccag tgagtttctt gccgacgacg
    10981 atcagcgcaa agtgaaaggt tacgatcacg cattcttgtt acaggccaaa ggcgatggca
    11041 agaaagtggc ggcgcatgtc tggtcagcag atgaaaaatt gcagctgaag gtctacacca
    11101 ccgctccggc tctgcaattc tactccggca acttcctcgg cggcacaccg tcgcggggaa
    11161 cagaacctta cgccgactgg caagggctgg cgttggaaag cgagtttctg ccggacagcc
    11221 cgaaccaccc tgaatggccg caaccggact gcttcctgcg tcctggcgaa gagtattcca
    11281 gcctgacgga atatcagttt attgctgagt aatgttaagc cctcgtcatg agggctttat
    11341 ctcatattgc tcaaatcacc agcaaacacc gacatatttg caactcaata ttcacaacaa
    11401 ccttacactg cgccactatt ttcgctatgg ttatgcgtaa gcattgctgt tgcttcgtcg
    11461 cggcaatata atgagaatta ttatcattaa aagatgattt gaggagtaag tatatggctg
    11521 taactaagct ggttctggtt cgtcatggcg aaagtcagtg gaacaaagaa aaccgtttca
    11581 ccggttggta cgacgtggat ctgtctgaga aaggcgtaag cgaagcaaaa gcagcaggta
    11641 agctgctgaa agaggaaggt tacagctttg actttgctta cacttctgtg ctgaaacgcg
    11701 ctatccatac cctgtggaat gtgctggacg aactggatca ggcatggctg cccgttgaga
    11761 aatcctggaa actgaacgaa cgtcactacg gtgcgttgca gggtctgaac aaagcggaaa
    11821 ctgctgaaaa gtatggcgac gagcaggtga aacagtggcg tcgtggtttt gcagtgactc
    11881 cgccggaact gactaaagat gatgagcgtt atccgggtca cgatccgcgt tacgcgaaac
    11941 tgagcgagaa agaactgccg ctgacggaaa gcctggcgct gaccattgac cgcgtgatcc
    12001 cttactggaa tgaaactatt ctaccgcgta tgaagagcgg tgagcgcgta atcatcgccg
    12061 cacacggtaa ctccctgcgt gcgctggtga aatatcttga taacatgagc gaagaagaga
    12121 ttcttgagct gaatatcccg actggcgtgc cgctggtgta tgagttcgac gagaatttca
    12181 aaccgctgaa acgctattat ctgggtaatg ctgacgagat cgcagcgaaa gcagcggcgg
    12241 ttgcaaacca gggtaaagcg aagtaaacgt cattcgttta aaatgagaaa gccgactgca
    12301 agtgagtcgg cttttttgtt gctaacaatg gagcacattg cctgatgcga cgcttcgcga
    12361 cttatcaggc ctgtggtgat tcatcggata cgccactctg acggcgcatc cgacaattaa
    12421 accttacccg cgacgcgctt ttactgcatt cgccagttga cgtaacagag catcggtatc
    12481 ttcccagccg atgcaggcat cggtgatgct cttaccgtag gccagcggct ccccgctctc
    12541 gaggctctga ttgccttcca ccagatggct ttccaccatc acgccaataa tggccttttc
    12601 gccaccggca atctgctggc aaacgtcagc acaaacatcc atctgctttt tgaattgttt
    12661 ggacgagtta gcatggctga aatcgatcat cacctgtgct ggcaggcctg ctttgttcag
    12721 cccttctttc acttcagcaa cgtgcttcgc gctgtagtta ggctctttac cgccgcgcag
    12781 aatgatatgg caatcgccgt taccgctggt attcacaatc gccgaatgcc cccatttcgt
    12841 tacggacagg aagcagtgcg gcgcaccggc ggcattaatg gcatcgatag ccactttaat
    12901 cgtaccgtcg gtgccatttt tgaagccgac tggacaagaa agccctgatg ccagttcgcg
    12961 gtgcacctgc gattcggtgg tacgtgcgcc aattgcgccc cagctcatca ggtcagcgag
    13021 atattgtggg gtgatcatat cgagaaactc acccgccgct ggcagaccgc tgtcgttaat
    13081 atcaagcagc aatttacggg ctatacgaag accgtcgttg atctggaagc tgttatccat
    13141 atgcggatcg ttaatcagcc ctttccagcc caccgtggta cgcggctttt caaaatagac
    13201 gcgcattacg atttccagct catctttcag ctcttcacgc agcgccagca agcgagtggc
    13261 atactcttta gccgcgacag gatcatgaat tgagcacggg ccaatcacca ccaacaggcg
    13321 atcatcatta cctttcagga tcttatggat cgcttttcgg gcatgggcaa ccgtattcgc
    13381 ggcattttca gtagcgggga atttttccag caatgcgaca ggaggaagta actctttgat
    13441 ttctttgatg cgtaaatcgt cgttctgata attcatgtct gttccagtgt tgccatactt
    13501 atcttagtga atgcaatact tccaatctat atcttccgtc agaatgtgta aacggggttt
    13561 tacattatga acggattaat cctggaatta gcaagaaaaa cgccagattg tcgcgaaaaa
    13621 cgagatctct cctacaattt ctaactgtaa ctcctttgtt tgttaattat ttcaagattc
    13681 tctgctgcgt ttcataaact ggctgaaatc ttaaaccaat gccttatatt cacctgcaaa
    13741 tgcactgttg gaagaggtta tccgacataa cgaccataac aggagcatcc tatgaaaatg
    13801 acaaaactgg ccacattatt tctgactgcc actctaagcc ttgccagcgg tgccgcactg
    13861 gctgccgata gcggagcgca aactaataac ggccaggcaa acgccgcaac tgatgcgggc
    13921 caggtagccc ctgacgcccg tgaaaatgtc gcgccaaata acgtcgacaa taacggggta
    13981 aataccggtt ctggcggcac aatgctgcat tcggatggtt cttcaatgaa caatgacgga
    14041 atgaccaaag atgaagagca caagaacacc atgtgcaaag atggtcgatg tccggatatc
    14101 aataaaaagg tacaaaccgg tgatggcatc aataatgatg tcgataccaa aaccgacggt
    14161 accacgcagt aaccctgcaa aatagccaat aatgggagag cggagtctct cccttttaat
    14221 ttttcacttc taaaagcagt atgatgtcat aaagttaagt gagaataatt aagggatgac
    14281 tcgtcatggc gcactcacac tcacacacgt cctcacacct gccagaagat aataatgctc
    14341 gtcgcttgtt gtatgctttc ggcgtgactg ccgggtttat gctggtagaa gtcgttggtg
    14401 gttttctttc tggttctctg gcattgctgg ccgatgcggg tcatatgttg accgatactg
    14461 ccgccctgct tttcgcccta ctcgccgtgc aattttcacg tcgtcctccc accattcgcc
    14521 acactttcgg ttggctaaga ctgaccacgc tcgcggcgtt tgtgaatgcc atcgccctcg
    14581 tggtaattac cattttgatt gtctgggagg cgatagaacg cttccgcacg ccgcgtccgg
    14641 tcgagggcgg catgatgatg gcaattgccg tggctgggtt gctggcaaat atactttctt
    14701 tctggttact tcatcacggc agcgaagaga aaaacctcaa cgtacgagca gcggcattgc
    14761 atgtgctcgg ggacctgttg ggttcggttg gggctattat tgccgcgttg attattatct
    14821 ggaccggctg gacacctgct gaccccattc tctcgatagt ggtgtcgctt ctggttctgc
    14881 gtagcgcgtg gcgactgttg aaagatagcg tgaatgaatt acttgaaggt gcaccggtat
    14941 cgctggatat cgctgaactg aagcgtcgta tgtgccggga aatcccggaa gtccgcaatg
    15001 tgcaccatgt acatgtatgg atggtaggtg agaagccggt gatgacgctg catgtgcagg
    15061 tgatcccgcc acacgctcac gatgccttgt tggatcagat ccaacactac ctgatggatc
    15121 actatcagat tgagcacgcc accattcaga tggaatatca accttgtcat gggccagact
    15181 gccacctgaa tgaaggggtg tctggccatt cacatcatca ccattaatgg gacagcgcgc
    15241 atgagcctct ttcacgtgcg ctgttgatcc acatccgact gccgttgagc gcaataaagg
    15301 tcaggatgat gtactccaga gacatggcgt aaacgccctg aagtgcaaaa ataacgacgc
    15361 taatcacgtt aataatcacc cacaacagcc agttttccac atacttacgc gtcatcagaa
    15421 gcattgccac gatagataac accatcatgc atgaatccca gaacgggaaa gcgtccggtt
    15481 gcagttcagg catcacaacc tgtaatccta atgcttgcat gatcatgact gccacgcggg
    15541 tcaaaaatgc aaacaccgga ttgataaata ccgtcatcag accaatcgaa acaacgcaaa
    15601 ccgccaacca gctgagcgcc ttcggcaatg gtaaccagcg aattttcaac tccgcctcgt
    15661 tctgactggt ttgtcgcgac cacgcatacc aaccgtaaat attcgcggca aagaaaaaca
    15721 cctgtaatag caggctggca tacagctgaa tctgaaagaa aataatgcca aacaaggtga
    15781 cgttgatcag gccaaagaag tagttgctga tcttctccag actggcaagg ccaatacaca
    15841 gcaacccggc gatcgtgcct accgcttcga tccatgagag atcataaccg cctgctccta
    15901 ttggtatatg taccaggata ttctgcacac taaaaaaatc catctttccc ccaaagcgta
    15961 acgttacgcc ttattattcg ttatccacgt agtgtagccg caaaatccag catacgattg
    16021 agcggcacca gcgccctctc tcgcagcctt tcatcaacat gaacctcgtg attgcttcct
    16081 tcctgttcta atgcctctgc gatggcctga aggccattca tggccatcca cggacaatgc
    16141 gcgcagctgc ggcaggttgc accctcacct gcggttggtg cttccagtaa ctctttatct
    16201 ggcaccgcct gctgcatttt gtagaaaata ccccgatcgg ttgccacaat aagcctctga
    16261 tgtggcaatg ttttcgcagc agcgatcagt tgactggtgg aaccgaccgc atccgccata
    16321 tcgacaatag cttgtggtga ttctggatgc accagtatgg cagcatccgg gtattcttct
    16381 tgcaagcggg ttaacgcctg agtcttaaat tcatcatgca caatacaggc accctgccag
    16441 catagaatgt ctccacccgt ctgtttttgc acgtaacgcc ccagatgttt gtcgggtgcc
    16501 cagatgattt tttcacccaa actatcaaga tgatcaataa gttcgacggc aatgcttgaa
    16561 gttaccaccc aatctgcgcg cgcttttacc gcagcagaag tgttggcgta gacgacgaca
    16621 gtacgatcgg gatgggcatc gcaaaatgcg ttaaattctt caacagggca gccgagatcc
    16681 agtgaacatt cagcctgaag tgtcggcatc agaattgttt tttccggact gagaattttg
    16741 gcggtttctc ccataaatct caccccagcg actaacaaag tagaagcggg atgctttgca
    16801 ccgaagcgcg ccatttccag agaatcagaa atacagccac cggtttcttc tgccagttgt
    16861 tgaatttcgg gatcggtata gtagtgggca accatcaccg cattacgttc ttttagcaga
    16921 cgttttatct tctcgcggta atacgctttt tcatcaatgc ttaacggcgt cggcttcggg
    16981 gggaaaggat aaatcgccgt gtctggatca aacattacgc tcatcttacc atctcgtttt
    17041 acaggcttaa cgttaaaacc gacattagcg taatattcgc tgttatcggt gtaatttgtt
    17101 ttatatgcta aacaagatag tcgaatggtg gggaaaagtc actcgaatta cgcctaattc
    17161 caccgacgta gagttgcttg cacagtaagc tctgacgatg tcatcaggtt tacaaagtga
    17221 aattgaggat gcctg
//
LOCUS       DAJVOY010000081        17095 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVOY010000081 DAJVOY010000000
VERSION     DAJVOY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.081"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..304)
                     /gene="hlyE"
                     /locus_tag="OQL90_002333"
     CDS             complement(<1..304)
                     /gene="hlyE"
                     /locus_tag="OQL90_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCS1369088.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLF"
     gene            677..1096
                     /locus_tag="OQL90_002334"
     CDS             677..1096
                     /locus_tag="OQL90_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1369089.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1096..2363
                     /gene="umuC"
                     /locus_tag="OQL90_002335"
                     /pseudo
     CDS             1096..2363
                     /gene="umuC"
                     /locus_tag="OQL90_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(2409..2939)
                     /gene="dsbB"
                     /locus_tag="OQL90_002336"
     CDS             complement(2409..2939)
                     /gene="dsbB"
                     /locus_tag="OQL90_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1369090.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3085..4626)
                     /gene="nhaB"
                     /locus_tag="OQL90_002337"
     CDS             complement(3085..4626)
                     /gene="nhaB"
                     /locus_tag="OQL90_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1369091.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4848..5567
                     /gene="fadR"
                     /locus_tag="OQL90_002338"
     CDS             4848..5567
                     /gene="fadR"
                     /locus_tag="OQL90_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1369092.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5619..7151)
                     /locus_tag="OQL90_002339"
     CDS             complement(5619..7151)
                     /locus_tag="OQL90_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1369093.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7481..8779
                     /gene="dadA"
                     /locus_tag="OQL90_002340"
     CDS             7481..8779
                     /gene="dadA"
                     /locus_tag="OQL90_002340"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1369094.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8789..9848
                     /gene="dadX"
                     /locus_tag="OQL90_002341"
                     /pseudo
     CDS             8789..9848
                     /gene="dadX"
                     /locus_tag="OQL90_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(10235..11971)
                     /locus_tag="OQL90_002342"
     CDS             complement(10235..11971)
                     /locus_tag="OQL90_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1369095.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12066..12980)
                     /gene="ldcA"
                     /locus_tag="OQL90_002343"
     CDS             complement(12066..12980)
                     /gene="ldcA"
                     /locus_tag="OQL90_002343"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1369096.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13080..13691
                     /gene="emtA"
                     /locus_tag="OQL90_002344"
     CDS             13080..13691
                     /gene="emtA"
                     /locus_tag="OQL90_002344"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1369097.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13693..14427)
                     /locus_tag="OQL90_002345"
     CDS             complement(13693..14427)
                     /locus_tag="OQL90_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1369098.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14628..14882
                     /locus_tag="OQL90_002346"
     CDS             14628..14882
                     /locus_tag="OQL90_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1369099.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            15061..15501
                     /locus_tag="OQL90_002347"
     CDS             15061..15501
                     /locus_tag="OQL90_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369100.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15580..17095)
                     /locus_tag="OQL90_002348"
                     /pseudo
     CDS             complement(15580..17095)
                     /locus_tag="OQL90_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         4308 a         4223 c         4419 g         4145 t
ORIGIN      
        1 caaatagcaa aatatacgct gcgagcaatt gcgtcgcaac accacaccat tcatacactg
       61 tttgggttgc ttcaaaatac ttatcctggc tatccataag taaggtttta atatcgccga
      121 ctaaaacgga ggctgcctgt gaatactcct gtttaaagcg acttaactct tttatggttt
      181 catcaaaggt ctgccagggg atgacctgat cgagatattt attataaaga tctaatgctc
      241 catctgcggt ttcgattgcg tttttaacta cttctaccat tttatctgca acgatttcag
      301 tcataatcat tcgcctcttt aaatatataa attgtaatga aactcctgtt ttacaactat
      361 taataaattt tacttcatct aattcatagt tagccgggtg ggatgcgtca atgtctttat
      421 ttctattaat atgataaata tcaaacaatg tttaatgtca ttatggcgaa tgcttctatt
      481 ctattttttt gccgggtgat atttttcatt tctgctggat gagcgtcgtc gccagaaggc
      541 cacgtgagca caggataaga gaacgaaaaa tcagcagtct atacagcgac aaatattgat
      601 agcctgaatc agtattgatc tgctggcaag aatagactac tgtatataaa aacagtataa
      661 cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt
      721 tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag attacgtcga
      781 acagcgcatc gatctgaatc aactgttgat ccagcatccc agcgcgactt acttcgtcaa
      841 agcaagtggt gattctatga ttgatggtgg aattagtgac ggtgatttac tgattgtcga
      901 tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg gcgagtttac
      961 ggtgaaaaaa ttgcaactac gcccgacggt acagcttatt cccatgaaca gtgcgtactc
     1021 gcccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga tccacgtcgt
     1081 taaggcgatg cgctgatgtt tgctctctgt gatgtaaacg cgttttatgc cagctgtgag
     1141 acggtgtttc gccctgattt atggggtaaa ccggtggttg tactatcgaa taatgacggt
     1201 tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggtg ttaaaatggg cgatccctgg
     1261 ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag caattatgag
     1321 ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc gccccgcgtc
     1381 gagatttaca gtattgatga ggcattctgc gatctgacag gtgtgcgtaa ttgtcgcgat
     1441 ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca tcttactgtt
     1501 ggtgtgggga tcgcccagac caaaacgctg gctaagcttg ccaatcatgc ggcaaaaaaa
     1561 tggcagcggc agacgggggg gtggtggatt tatcaaatct ggaacgccag cgtaaattaa
     1621 tgtctgctct ccccgtggat gacgtctggg ggattggacg gcggatcagc aaaaaactgg
     1681 acgcgatggg gatcaaaacc gttcttgatt tggcggatac agatatccgg tttatccgta
     1741 aacattttaa tgtcgtgctc gaaagaacgg tgcgtgaact gcgcggtgaa ccctgtttgc
     1801 aactggaaga atttgcaccg acaaagcagg aaattatctg ttcccgctcg tttggtgaac
     1861 gtatcacgga ttatacgtcg atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg
     1921 aaaaacttcg cagtgagcat caatattgtc ggtttatctc cacatttatt aagacgtcac
     1981 catttgcgct caatgaacct tattacggca atagcgcgtc ggtaaaactg ctgacgccca
     2041 ctcaggacag cagggatatc attaacgccg ctacgcgatc tctggatgcc atctggcaag
     2101 cgggccatcg ctaccaaaaa gcgggcgtga tgctggggga tttcttcagt cagggagtcg
     2161 cgcagctcaa tttattcgat gacaacgcac cgcgccccgg gagtgagcaa ttgatgacgg
     2221 taatggatac actgaatgct aaagagggca gaggaacact ctattttgcc gggcagggga
     2281 tccaacaaca atggcagatg aagcgagcca tgctttcacc acgttataca acgcgaagtt
     2341 ctgatttact gcgggtcaaa taaatatagc ggcaggaaaa aagtgctccc gcaggagcgc
     2401 cgaatggatt agcgaccgaa cagatcacgt tttttcgctt taaacggctg ggaaatcatc
     2461 accagcaccg cgacaatcag gtaagcgata aaaataccga gcagccactg cggcatttcc
     2521 agacctaaaa aatcccactg acgctcggcg caatcgccag aggcgacaaa cacttgcggc
     2581 acccatttat ccagcggcag ccattccggg aaacgaacca taaaatcaca ggtggcaaac
     2641 ggcgaaggat agagctgaag catggtgtgc tcgtaagtta actgcacacc gcggaacgca
     2701 ctatacaacc agataaccat cgctacataa cgcagcggag ttttcggggc gatcgcgcca
     2761 atcagcgcag cacccagaac gccgaataac gcgcagcgtt cataaataca gagcacgcaa
     2821 ggtttcagta acatcacatg ctggaaccac agcgccgtca gttccagtgc cagagcagta
     2881 aacgccatca acagccacgc gccccggcct tgtgaacatt ggttcaaaaa tcgcaacata
     2941 atcatttccc tgcaatatgc atagagtgcg cagtttaaac caattcattc gctgcgccac
     3001 caggaggcag caaaaatagg acgcaattgg acaattatcg ggcgaaaagg caaaccgggc
     3061 atttatatgc ccggtaagtt gttatcaaag cgttgctatc cagcccattt gcataaacca
     3121 ttcggttaca ggggcaagcg taaactcgac gcagagcagc ccgacgagtg tcaggacgag
     3181 ggtgtaaggc agggccatcc acaccatgcg gccataagag aggcgaatca atggcgcgag
     3241 tgcagaggtc agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga cagagggcag
     3301 attggtaccg gtattaatgg cgaccgccag cagctcgtat tgcttcaacg tgatagcgcc
     3361 actttccatt gccgctttcg cttcgttgat ataaatcgtc cccacgaaga cgttatccga
     3421 aatggatgac agcagaccgt tgaaaatata gaacaacgac agctgagcat gttccgatgc
     3481 ctgcaacaca aactgaataa tcggcgaaaa cagttgttgg tcgataatca ccgcgactac
     3541 cgagaaaaac accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg ctttaccgat
     3601 agcatgttca tcggtgacac cggtcaatga tgtcgccaga ataatgaccg acaaaccaat
     3661 caagccaacc tccgccagat gcaacgccag cgcagtcacc agccagacgc cgataatcgc
     3721 ctggacaatc agacggattt tatcctgacg ggtacgctgg tggcggcttt gatcgtcaaa
     3781 ctgttgcaac acttcgcgga ctttctccgg cagcgtttca ccgtagccaa accaacgcag
     3841 cttctctacc agcaggcagg ttaacaggcc acaaatcaga accggaacgg tcaccggcga
     3901 catgcgcagg aagaaatcgc caaaatgcca gccagccgct ttagcgatga tcaggttctg
     3961 tggttcgccc accatggtca ttacgccgcc taatgcggtg ccgacaccgg catgcatcat
     4021 caggctacgc agaaagccac ggaactgttc cagaaccact ttgtaatgct tgtcgatatg
     4081 actatcgtct tgcaggtcgg tatcttcagt acgggaagag gctacgcggt gataaatacc
     4141 ataaaaaccg actgcaacgc tgatcaccac cgccacgacg gttaaggcat cgaggaacgc
     4201 ggagaggaac gccgccgcca cgcaaaaaga gagcgacagc agcattttgg aacgaatgct
     4261 taacagcaaa cgggtaaata tgaacagcaa tagctgtttc ataaaataga tacccgccac
     4321 cataaacatc agtaacagca agacttcaag atttgccgcc acctcttcac ggacgtgttc
     4381 cgcgctggtc atgccgatga ataccgcttc aatagccaac agaccaccgg ggagtagcgg
     4441 gtagcatttc agggccatcg ccagagtgaa aataaattcc gctaccagca accagccagc
     4501 gacgaaaggg ctgatgagga aaattaacgg atttacgatt aagaaaatga tgagggcgag
     4561 tttgtaccag tcgggggact ggcccaaaaa attgcgccat agcgcgcggc cccaggagat
     4621 ctccatgatg gtttccctta ccttacaaat aatcaatgat gtttttatgt ttaaacgcaa
     4681 agcttaacgg tcaggcagga gtgaggcaag tcttgatagt caaggggaaa gagatgcgga
     4741 aaatgaagcc ttgatccctt ttttcttctt tttgtctgct atcagcgtag ttagccctct
     4801 ggtatgatga gtccaacttt gttttgctgt gttatggaaa tctcactatg gtcattaagg
     4861 cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg aataaccgct
     4921 tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt ggcgtaacgc
     4981 gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg accattcaac
     5041 atggcaagcc gacgaaggtg aataatttct gggaaacctc tggtttaaat atccttgaaa
     5101 cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg ctgtcggtgc
     5161 gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc gataaagcgc
     5221 aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt gccgagctgg
     5281 attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac ggtctgatcc
     5341 ttaacgggat gaaagggctg tatacgcgca ttggtcgtca ctatttcgcc aatccggaag
     5401 cgcgcagtct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt gaaggcgcgc
     5461 acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag atttggcacc
     5521 ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatcc cttccgttta
     5581 aaaagcaaac ccctcaaacg aggggttttt tgttgttttt acagatttcc cattcttggc
     5641 gggcaacgtt ccagcaactc gacgctgcca tcttcgtttt gctgttccag catcacatca
     5701 aatccccaca ggcgatgcac atgcttcagc acttctttgc gcccccgatc cagcggtgcg
     5761 cgattatgtg gaatgtaacg cagcgtcagc gaacggtcgc cgcgcaaatc cacgttccag
     5821 atctgaatat tcggctccag attacttaag ttatattgcg acgataaccg gttacggatc
     5881 tcccgataac cttcttcatt atgaatagcg gaaatctcca gataattatg ccgatcgtca
     5941 tccagcacgg tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa ctggctgata
     6001 aaactctcat ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca gtcggaaccg
     6061 gcgatatccg ggaaccagta tttgtcttct tccgttggcg actgacaaat ccgtttaata
     6121 tcctggaaca tggcgaaccc gagggcatac gggttgatgc cgctgtacca cgggctgtta
     6181 taggggggct ggaagaccac attggtgtgg ctgtgcaaaa actccagcat aaaacgttcc
     6241 gttactttcc cttcatcata cagatggtta aggatggtgt agtgccagaa ggtggcccag
     6301 ccttcgttca tcacctgagt ctgtttttgc ggataaaaat actggctcac cttacgcaca
     6361 atacgcagga tttcacgctg ccatgattcc agcagtggcg catttttctc cataaaatag
     6421 agcaggtttt cttgtggttc ggacggatag cggcgcgctt cagcaaccgt tttctcttcc
     6481 tcgcgcttcg gcagggtacg ccagagcata ttgacctgac tttgtagata ctcttcgcga
     6541 cttttctgcc gggctttctc ttcttgcagc gagatttttt gcgggcgttt gtaccggtcc
     6601 acgccgtagt tcatcagcgc gtggcacgag tccagaagcc gttctacttc atccacgcca
     6661 taacgttctt cgcactcggt aatatattta cgggcaaaaa tcagataatc gacaatcgaa
     6721 ctggcgtcgg tccaactgcg gaataagtaa ttgtttttaa agaaagagtt atgcccatag
     6781 caggcgtgtg ccatcaccag cgcttgcatg gtaatggtgt tctcttccat caggtaagcg
     6841 atacacgggt tagagttaat gacgatttca taggccagtc cttgctgacc gtgcttatac
     6901 agccgttcag tctcgataaa ctttttaccg aatgaccagt gcggatagtt aattggcatg
     6961 ccgacgctgg agtaggcatc catcatctgt tctgaggtta tcacttcaat ctggtgcggg
     7021 taggtatcca gccggtagag tttcgccacc cggtctatct ctgccagata aacatccagc
     7081 aggtcgaacg tccagtcggg tccatcgctc aaccgtgtgg tgtccttatt catagaatcg
     7141 atcgtcgcca tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc acctcatttc
     7201 aagcatagaa cacctgttaa aaaccgcgtc gccggagaat ttttttcttt gcgatttctt
     7261 attatcagag tgccactaat ccgcttctga acggaatttt atgctggata aaaagggcgt
     7321 tcagcaggag atactaaaga cgccatattg ccgcagagtc agggagatgt gagccagctc
     7381 accataaaaa agctgtatgt tgaataatat tttcaactga gttatcaaga tgtaattaga
     7441 ttattattct tttactgtat ctaccgttat cggagtggct atgcgagttg tcatactggg
     7501 aagtggtgtg gtaggcgttg ccagcgcctg gtacttaaat caggcaggac atgaggtcac
     7561 cgtcattgat cgggagccgg gggccgccct ggaaaccagc gccgcgaatg ccgggcaaat
     7621 ctcccccgga tatgctgcac cgtgggcggc accaggtgtg cctttaaaag cgatcaaatg
     7681 gatgttccag cgccatgcgc cgctggcggt tcgtctcgac ggtacgcagt ttcagttgaa
     7741 atggatgtgg caaatgttac gtaactgcga caccagccac tacatggaaa acaaagggcg
     7801 gatggtgcgt ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg ccgaaaccaa
     7861 tattcagtat gaagggcgtc agggtgggac gctgcaactg ttccgtaccg aacaacagta
     7921 tgaaaatgcg acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt atcagctgct
     7981 ggaatccagc cgcctggcgg aagtggagcc agcgctggca gaagtagcgc acaaactgac
     8041 gggtggtctg caactgccca atgatgaaac cggagactgc cagctgttta cccagaatct
     8101 ggcgcggatg gcagagcagg cgggggttaa attccgcttt aatacgcccg ttgaccaact
     8161 gctttgcgac ggcgagcaaa tctacggcgt gaagtgtggc gatgaagtga ttaaggccga
     8221 tgcgtatgtg atggcgtttg gttcttactc gacggcgatg ctcaaaggca ttgttgatat
     8281 tccggtgtat ccgctgaaag gctactcgct gactattcca attgcgcagg aagatggtgc
     8341 gccagtatcc accattcttg atgaaaccta caaaatcgcc attacccgtt tcgataaccg
     8401 cattcgtgtt ggcggaatgg cggaaattgt tggttttaac accgagctgt tgcaaccgcg
     8461 tcgtgaaacg ctggaaatgg tggttcgcga tctctatcca cgcggcggtc atgtcgagca
     8521 ggcgactttc tggacgggtt tgcgcccgat gacgccagac ggtacgccgg ttgtcgggcg
     8581 tacacgcttt aaaaatctgt ggctgaatac cggtcacggc acgctcggct ggacgatggc
     8641 ttgcggttcc ggtcagttgt taagcgatct gctctctggt cgcacgccag cgatcccata
     8701 tgaggatcta agcgtagcgc gctacagccg tggatttacg ccatcacgtc cgggccattt
     8761 acatggcgca cacagctaag gaaacgagat gacccgtccg atacaggcca gcctcgatct
     8821 gcaggcatta aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg cgggtggtaa
     8881 aagcgaacgc ttacgggcat ggtattgagc gtatctggag cgcgctcggg gccaccgatg
     8941 gctttgcatt acttaacctg gaagaggcaa taacgttacg tgagcgcggc tggaaggggc
     9001 cgatcctgat gctggaagga tttttccatg ctcaggatct ggagatttat gaccagcacc
     9061 gcctgaccac ctgcgtacac agcaactggc agctcaaagc actgcaaaat gcgcggctaa
     9121 aagcaccgtt ggatatttat cttaaagtga acagtgggat gaatcggttg ggcttccagc
     9181 ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat ggcgaatgtt ggcgagatga
     9241 ccctgatgtc gcattttgcc gaagcggaac atcctgatgg aatttccagc gcgatggcgc
     9301 gtattgagca ggcggcggaa gggctggagt gtcggcgttc gttgtccaat tcggcggcga
     9361 ctctgtggca cccggaagcg cattttgact gggttcggcc tggcattatt ttgtatggcg
     9421 cttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg attacgtccg gtgatgacgc
     9481 taagcagtga gattattggt gtccagacgc taaaagcggg tgagcgtgtg ggctacggcg
     9541 gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt cgccgcaggg tacgccgacg
     9601 gttatccgcg ccacgcgcct accggtaccc ctgttttagt ggacggcgtg cgcaccatga
     9661 cggtggggac cgtctcgatg gatatgctgg cggtcgattt aacgccttgc ccgcaggcgg
     9721 gtattggtac gccggttgag ctgtggggca aggagatcaa aattgatgat gtcgccgccg
     9781 ctgccggaac ggtgggctat gagttgatgt gcgcgctggc gctacgcgtc ccggttgtga
     9841 cggtgtaact tgttgtaagc cggatcggag gcaacgtctt ctgggtgcaa aaaaaatcat
     9901 ccatccggct ggtcagcaac tgtagttgtt aatgtgacag agccattgcc catgatagtg
     9961 tccattaaaa ggatggacac tatttccccg gaacctgaac tcaccgcaca ggcgttctac
    10021 ataaaacgct tacgcttcat tgttgactcg gagtcgactt cagataaatc acgctttacc
    10081 cttgatggag cctgtacata gatttgtgta attgcctgat tttgatatgt tcaattcaac
    10141 atcaaatgaa ggttaaatta tggacgacaa acaattgcag gctcaggctg cgttcagcaa
    10201 agcatcgcaa ccggcgatag atgcttcatt aaatttaaga ttcagcttcc tcttcagcca
    10261 cccgtacgcc aatcttcaac acttcattat cttctttctc ggccaccgtc cagatcatcc
    10321 cggcaaactc tacctggtca ccgacaaccg gtgccgtgcc taacaactgc tggacaattt
    10381 cacccagcgt ttgctgctta tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg
    10441 caacatcagc atatttggcg ctggcttcga gaatgaagtc accaaagaag cgttgatcca
    10501 gcgcgaccgg cggcgactgg ctgaacagtt taccgagcgc cggaagatcg cgttcccgac
    10561 caattacaca caacacatcg ccttcgcgca gtcgggtgct gccggtagga tgaagcaact
    10621 ggttatcacg aaacagtgcc gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg
    10681 ccgcgcccac gcaccattta tcggcactca attgataaac aaactgctcc cacggatttt
    10741 ccggatgaat atccaggcca acgcgtgaca ccggacgtcc cactggcgga accaccactt
    10801 tggctttttt agccgcccac gagagtgatg ttccctgcaa gagcagtgaa accagaacca
    10861 caaagaaggc gacattaaag aacagacgtg cattctccag ccccgccatc atcgggaaca
    10921 cggccaggat gattggcacc gcgccgcgta atcctaccca gctgataaac acgcgctcac
    10981 gcagattgaa accgcggaag gggagcaatc cagcaaatac cgaaagggga cgggcgaaga
    11041 atatcatcca tgccgacaaa attagcgccg gaatggcaat aggcagcaga tcgcttgggt
    11101 taaccaatag ccccagcacc aggaacatgg cgatttgcgc cagccaggcg aggccgtcga
    11161 aattttgcag gatgccgtag cggttgcgaa tcggccgatt acccagcaga aaaccgcaca
    11221 gataaacagc cagaataccg ctgccttcca gcgccgtggt taaagcaaaa attaaaatac
    11281 cgccgcttaa tgccagcaat ggatataatc cggcaggcag ggcgatgcga ttaatcattt
    11341 gcagcagtaa ataaccaccg ccaagtccga tgacaatccc gaggccaaat tgctgcagaa
    11401 tatcgaccac gaacatccaa ctgacactgc tttcatgttg ctggatcatc gcaatcaggg
    11461 taatcgtcag aaagaccgcc attggatcat tactgccgga ttcaatttcc agcgtcgagc
    11521 caacacgttc gttaagcccc ttaccaccca gcagagaaaa gaccgctgca gcatcggtag
    11581 agccgacgat agcgccgatt aataagcctt caatcaaatc aagattaaac agccacgccg
    11641 ccatcatgcc ggttaaacca gaggtgataa gcacgcccag cgtcgccagc gacagtgccg
    11701 gtcctaacgc cacacgaaag gaactggcct gagtgcgcat cccgccgtcg agcaaaataa
    11761 tcgccagtgc caggttactt accatgtagg cgaaggggta attatcaaac gggataccgc
    11821 cgacgccatc gactcctgcc agcatgccga tcgccagaaa aataaccaga ataggaatgc
    11881 caagacggga agaaaatgaa ctaagtaata tactgctggt gacaaggatg gatcctaaga
    11941 tgaaaaggct aattattgtt gtggcatcca acggtcggtt actcctgatt acgctgtctc
    12001 ttatataaac cctaccatat tagcggcaga gacagcgttt tacttagccc tgaagcgtga
    12061 tttttttaca ttttaagaac aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg
    12121 ttattcagaa tggcatgtgc gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa
    12181 tcgaggccgg taatgagtgg aatcgacagg cgggaacgca aaaacgcgga cactgactcc
    12241 aggttgtaac ctgcgtcata atcattgggc gtgctgccgc taaagctacc gagaataatc
    12301 gccttctgac gtggcaaaat tcccgcatga tagagctgca acagcatacg ttcgacgcgg
    12361 aaagggtgct cgttaatatc ttccagtacc aggataccgt tctcaatttt tggcatccac
    12421 ggtgtaccaa tcagtgaaat cagcatcgca agattgcctc cccacaacgt gccttcggtc
    12481 tgacatgtcg ggccttcccc ttgccattca atggtgaagg ttttattgcg taacgccagc
    12541 cagaaatggt gctcggtaaa ggcgttcagt tcatccgcac caaagtttgc taccagcatg
    12601 gggccgctaa aggtgatgac attgccttgc gccagaagac cgcactgaat ggcggtaaaa
    12661 tcgctatgtc cgcaaatgag taacgggtca tgttgttggc gagccaccag tgcctgccag
    12721 tcaatatctg ccagtaaacg actggcaccg taaccgccgc gtacagccag cacgatggtg
    12781 ttgggtgtag tcagtctggc aagggaattg agatcttcca gacgctccgt ttccgtaccc
    12841 gcaaaacgct cacaacgacg ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg
    12901 gtaaggcgct ggataccacg cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca
    12961 attaagtgaa acagagacat ggcaattcct tgctgacaac agaaacgaaa tgtatatcat
    13021 gccgcttagg tgtgccgttg tcacctcaac ggcgattcca ggctataagg atagaagaag
    13081 tgaaattgag atggtttgcc tttttgattg tgttattagc gggttgttca tcaaagcatg
    13141 actatacgaa cccgccgtgg aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc
    13201 caataagcca gaaagccggt gcagcctggg gcgtcgatcc acaattgatc acggcgatta
    13261 tcgctatcga atcgggtggt aatccgaacg cggtgagtaa atcgaatgcc attggtttga
    13321 tgcagttaaa agcttcaacc tccggacgtg atgtttatcg ccgtatgggc tggagtggtg
    13381 agccgacgac cagcgagctg aaaaatccag agcgtaatat ttcaatgggg gcggcttacc
    13441 tgaatattct ggaaaccggc ccgctggcag gcattgaaga tccgaaggta ctgcaatatg
    13501 cgctggtggt gtcatacgct aacggggcag gtgcgctgct acggactttc tcgtcagatc
    13561 ggaaaaaggc gatcagcaaa atcaacgatt tggatgctga cgagttcctc gatcacgtag
    13621 cgcgcaatca tcctgcgccg caggctccgc gctatatcta caaacttgag caggcactgg
    13681 acgcgatgta aatcagtcgc gtactttgtc cgctttctcc cgggcttccc gctcgagaga
    13741 gaaaataatc cgttgtaatt gccgctccac cgtcgggctg acgttaagaa aacggaagct
    13801 cagacgggga gtggtgatgg tttcattctt gccatcaatc actttgcgct cgctgatgga
    13861 gattaactgg gcgtcaaagt gaaaaacacc ccattgcccc atgttgactt caatctgagc
    13921 gaagcgcatg ccttcttgta attcggcagg ctttgctgtt tccagtaatg cgcccatgcc
    13981 gcctaacgac aaatcataca ggcggaaacg taacgtactg ttatccgcca gtttggtctg
    14041 gcaaaaataa ggcggatgga gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa
    14101 ccataaggtg ggaggcggta cggtaataaa tgccggaagc tgcaagtatt cactctgctg
    14161 tagttgttca acagtaaact cgactttcgc accctgagtt tcggcggtaa tggtaatatg
    14221 ctgcgccttt agcacggcga tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt
    14281 atccggggtt attgccagta ttttgctgat cagctgcccg ccattccaac tgagacgcaa
    14341 aggaattgcg gctttgtgca aatcgcgtaa cacgcccagg acggctaacg gattttgttt
    14401 caggaactgc tcatggtaat gactcacgcg gaaaaactcc tgactgacaa actgtctttg
    14461 gtttatcggt cacagttaac aaaacttaat acaaatgcgt gaatattttt tacatgttgt
    14521 tcttaaatca gccgacatac gcccagcatc gatcccgccc ttgcctatac ttagagcgtt
    14581 gacgtaagca tttcttgcgt ccgattcatc gaaacgaggg cttgaacatg ggaattattg
    14641 cctggattat ttttggcctg atagccggca ttatcgccaa gctaatcatg ccggggcgtg
    14701 atggtggtgg atttttccag acctgtattc tcgggatagt cggtgcggtg gtcggcggct
    14761 ggctggcgac catgtttggc attggcggct ccatcagcgg ttttaatttg cacagcttcc
    14821 tggtggcggt ggtgggagct attctcgttc tgggcttatt ccgcctgctg cgaagagaat
    14881 aagattttca taaggcggat agcgatacag atgccgctat ccgcttttca catcagaacg
    14941 tatattcgac acctgcttta cgggtgaaaa aaatcaatat agcacttcga tatccgataa
    15001 agtcggttcc cggtaacaac aacagaatcc ttttttaatt attgtttcgt tgtttttggt
    15061 gtgattcaga aaataaatat tttagacagt agccagtcag gcgttaaaaa tggggcggat
    15121 agtgaggcgg ttttggctgc gcttattgaa catttagaat tgatcaatcc ttctggcaga
    15181 ttaagccaaa atacccgttc ggcaatgctt cagttacgcg aagaggaatg gtctgaattc
    15241 tttttttggt tgctaaattc tctggagtgt ctggattatg tgattattaa tttaactcca
    15301 gaaagcaaaa aaacgttgat gagtgaacac cgtaacaata ttcaggtagc aatcgatgct
    15361 ttatataggc aacgtcggtg taaatcaccg ggggatgata gtgaaacact tacccgtaga
    15421 aatgacgcta tcttcggcaa ccacgtatgg caaacatttg cgcaatactt cccgccagga
    15481 cttgaaaaac cggctgtgta gaaaacacca tagctatggt gttatctgcc gacaacgcca
    15541 tccggagaag ccgaatgacg ttgtcggagt aagcgctggt taaggtgtgg gttgtgcctc
    15601 tttggttgag ggttgcgtcg ttgctgactt aacggtcgga cgcgtcgccg gaacattgtc
    15661 acacggttgc tctttcgggc agatcaaatc cagcattttc agcgtcacgc cattggtcca
    15721 gccaaagcca tcctgtaatg gatattcgcc acctcttttt gcccgtagtt ttgtaatcct
    15781 tctgtcgcga cccactgtaa cggtgcccag ccatttggcg catcccattg ttgcccactt
    15841 ttcaccgacg tggtgttcag gccgccgggt tgcagcagat gtgttttcgt cgccgtcgcc
    15901 attttgttgg cgcgatcttt cgctgccgca ttgacgtaca gcgggaacag ggcggccgcg
    15961 gttaactgat tgcgcacttt atgacttttc aggtcgtaat cggcatacca gccttgttga
    16021 tcgttccaca ggtatttttc gattcctttt tgacgggcat tcgccagcgt ttcgtactgg
    16081 tttgccatcg cgttatctcc tgcagctttg ctggcgcggg cgaggatttt ttccatttta
    16141 aacatcaggc tgttcagatc gaccggtacg atgctggtgg tgcgtaaggt atttaactgc
    16201 tgcgggttgt ccatccagcg cgagctgaaa tcccagccag acgcagcggc agagcgcagg
    16261 tcgcggtaaa tttcagtggc aggtcgattc ggattgcttt tggcggtggc aatatcttcc
    16321 acccatgact ctggtcgtgg cgtatcgcga tcgtcccagt agcggttgag aagggtacca
    16381 tcctgaagtt tgacaacgcg tttttcctgt tgtccggctt gcaggttttc aacaccgtcc
    16441 atccagtaag catattcttt ttgcatttgc ggcaggtatt gcttcaacgc ggcatcgcct
    16501 tcatgctgcg ccagtaactc taccatcagg gcaaagaagg gcggttgcga gcggcttaaa
    16561 tagtaactgc ggttgccgtt gggaatatga ccgtaagtgt ctatttcatg agcaaaattg
    16621 gccaccatat ccgcgacttt atcccagtga ccgctttcag caagtcctaa catggtgaag
    16681 taactgtccc agtaatatac ctcgcgaaaa cgtccgcccg gcacgacata aggttccggc
    16741 agcggtaaca gagaatccca tttttcggtg ttttcggtag aacgcgttaa taccggccaa
    16801 agtccgtcaa tatgttcgcg cagtgactgc ccctctggcg gaacatattt ctcgccttct
    16861 ttcggcaggg tgaaattgac gttaacgaaa tggcgcagat caaatccgct ctggttttgc
    16921 tgcatccgat aatcagcgga tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg
    16981 gaaaaagttt ggcgttttgc acatcattaa acagcggccc taataaaata tcaggcggct
    17041 atggtgttac cggtgtttct tctgcctgca ccgatagcgc agcgaaacac aaaaa
//
LOCUS       DAJVOY010000082        16749 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVOY010000082 DAJVOY010000000
VERSION     DAJVOY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.082"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..2017
                     /gene="fhuE"
                     /locus_tag="OQL90_002349"
     CDS             <1..2017
                     /gene="fhuE"
                     /locus_tag="OQL90_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCS1369101.1"
                     /translation="DYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLEISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="OQL90_002350"
     CDS             complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="OQL90_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1369102.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3805..4602)
                     /locus_tag="OQL90_002351"
     CDS             complement(3805..4602)
                     /locus_tag="OQL90_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1369103.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4613..5617)
                     /gene="holB"
                     /locus_tag="OQL90_002352"
     CDS             complement(4613..5617)
                     /gene="holB"
                     /locus_tag="OQL90_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1369104.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5614..6255)
                     /locus_tag="OQL90_002353"
     CDS             complement(5614..6255)
                     /locus_tag="OQL90_002353"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1369105.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="OQL90_002354"
     CDS             complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="OQL90_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1369106.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="OQL90_002355"
     CDS             complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="OQL90_002355"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1369107.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="OQL90_002356"
     CDS             complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="OQL90_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1369108.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="OQL90_002357"
     CDS             complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="OQL90_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1369109.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="OQL90_002358"
     CDS             complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="OQL90_002358"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1369110.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="OQL90_002359"
     CDS             complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="OQL90_002359"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1369111.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11667..12620)
                     /gene="fabH"
                     /locus_tag="OQL90_002360"
     CDS             complement(11667..12620)
                     /gene="fabH"
                     /locus_tag="OQL90_002360"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1369112.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="OQL90_002361"
     CDS             complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="OQL90_002361"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1369113.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="OQL90_002362"
     CDS             complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="OQL90_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1369114.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="OQL90_002363"
     CDS             complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="OQL90_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1369115.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14784..15368
                     /gene="yceF"
                     /locus_tag="OQL90_002364"
     CDS             14784..15368
                     /gene="yceF"
                     /locus_tag="OQL90_002364"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1369116.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="OQL90_002365"
     CDS             complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="OQL90_002365"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1369117.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         4159 a         4546 c         4047 g         3997 t
ORIGIN      
        1 tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacgtgatat
       61 tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct
      121 gggcgaagtg atggaaaaca cgctggagat cagcaaaagt caggcggatt ccgatcgtgc
      181 tctttattat tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta
      241 ttttgaatcg cgctggaatc tgggcgatgc actttctgat atggcactgt ttgaacgcgt
      301 agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat
      361 tgatatggtt cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata
      421 cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg
      481 taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta
      541 caacagtgaa aagaccttct tctccggcat tgtcgatgct gatttaggcg atcttacgac
      601 gctttcagcc ggttacgaat atcagcgcat tgatgttaat agccctacct ggggcggttt
      661 accgcgctgg aatactgatg gcagcagcaa cagttacgat cgtgcacgca gtaccgcacc
      721 tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt
      781 tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat ttgacagcaa
      841 aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag
      901 taattatgga cctggctttg attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa
      961 agttgatgcg ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa
     1021 tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tacttcagtt catgggccaa
     1081 catcttcccg gatgaaattg gcagtttcta caactttaat ggcaatttcc cacaaaccga
     1141 ctggtcacca cagagcctgg cacaggacga taccacacat atgaaatcgt tatatgctgc
     1201 cactcgagtc acccttgccg atccgctgca tctgatcctc ggcgcacgtt ataccaactg
     1261 gcgggttgat acgctgactt acagcatgga gaaaaaccac accacgcctt acgctggtct
     1321 ggtgtttgac atcaatgaca actggtcgac ctacgccagc tatacctcta ttttccagcc
     1381 gcaaaatgat cgtgacagtt caggcaaata tctggctcca atcaccggta acaactacga
     1441 gctgggtctg aaatcgggct ggatgaatag ccgtctgacc accacgttag ccatcttccg
     1501 tattgagcag gataatgtcg ctcagtccac cggtacacct atccccggca gcaacggcga
     1561 aaccgcctat aaagcggtgg atgggacagt cagtaaaggg gtggaatttg aactcaacgg
     1621 cgcaattacc gacaactggc agctgacatt tggcgcaacg cgctatattg cagaggataa
     1681 cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta
     1741 tcggttgcct gtcatgccag agttgacagt cggcggtggt gtgaactggc aaaatcgcgt
     1801 gtataccgac accgtgacac cgtatggcac cttccgcgcc gagcaaggta gctacgcgct
     1861 ggtggatctc ttcacccgct accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa
     1921 taacctgttc gacaaaacct acgataccaa catggaaggt tctatcgtct acggcgcacc
     1981 gcgtaatttc agcattaccg gcacgtatca attctgaata acacctgtaa aaaaggcagc
     2041 catctggctg ccttagtctc cccaacgtct tacggattag tggttacgga tgtactcatc
     2101 catctcggtt ttcaggttat cggatttagt accgaaaatc gcctgaacac cagaacccgc
     2161 aaccactacg cccgctgcgc ccagtttctt caggccagcc tgatccactt tagacacatc
     2221 agcaacgctg acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt tttctttacc
     2281 accaaatgca gcaaccagag ccggtgccat ttcgctggta cctgttgctt ttgcatcttc
     2341 agtcgcgtct tcacgacccg gcgttttcag atccagtgct ttaatcagca cgcggaagat
     2401 ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg aacagccaca gtttgctgct
     2461 gttaccagac agaacgataa agtcgatcag accgtgcgag aacgacgtac cgtcatgcat
     2521 ccccagaaga atacagattg ggaatgccag gcctgccaga atcgcgtgga tgatgtacag
     2581 gatcggcgca acgaacatga aggagaactc gatcggctcg gtgataccgg tcaggaacga
     2641 ggtcagcgcc gcggagatca taataccgcc cactttcgcg cggttttctg gtttagcaga
     2701 gtgccagata gcaattgcgg cagctggcag accgtacatt ttgaacagga agccaccaga
     2761 cagtttaccc gcagtcgggt cacccgccat ataacgcgga atgtcgccgt ggaaaacctg
     2821 acctgctgcg ttggtgtatt caccaatctg catctggaaa ggtacgttcc agatgtggtg
     2881 cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg caactaccgg
     2941 gttctggtaa gcagcccact gagagaaggt ctggattgca gaaccaatcg gcggccaaat
     3001 gaaggacagc acaacgccag taaagatggc agccaggcca gaaatgatcg gcacaaagcg
     3061 tttaccggca aagaagccaa gatactcagg cagcttaata cggtagaaac ggttaaacat
     3121 gtacgctgcg atcgcaccgg agataatccc tccgagtacg ccagtatccg ctaggtgttt
     3181 agaggcgatt tcttcagcag gtaaatgcag taccagtggc gcaaccacgg ccatggtttt
     3241 aaccatgatg ccataggcaa caactgcggc cagcgcggat acgccatcgt tattggtaaa
     3301 gccgagggcg acaccgatcg caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc
     3361 ttctgccata acatgcgata caacggcggg cagccagctg aaattcgcgg aaccgacgcc
     3421 cagcagaata cctgcgatag gtagtacgga taccggcagc atcagcgatt taccgacctt
     3481 ttgcaggtta gcaaatgcat tcttaaacat aattgagagt gctcctgagt atgggtgctt
     3541 tttttacgtt ctcacgcatg gcaagggggg agagcctcgc cgtgtacagg gcatctaagc
     3601 gccctttatt tattacacag agtaaaataa ttcagtgcca atttgtttga cggctatcac
     3661 gtttcaactt cagatcattg gagaagttgc acattttgac aaaacatatg tcacaatatt
     3721 tcaaacgcgg ttattcaccg cccaattttc ggcggtgaac tttactcgtt ttagccatta
     3781 attacgagct ttaaaaaaac tcattcaacg gatggattga aggcgggaag cgtcgatgtg
     3841 aaacagacgg gcgaagttat cggtggttac ctgcgccagt tcttcaacgg caacaccttt
     3901 caacacagcc atgtattctg caacgtcacg aaccatcgca ggttgattct cttttcctcg
     3961 atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat ccagggggac
     4021 ataacgcgca gcatcgcgca gttgctccgc attacggaag gtcacaatgc cggaaaagga
     4081 gatgtaaaat ccgagatcca gtaatttacc cgccgtttct ctgtcctctg taaaacagtg
     4141 tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga attgccagcg tatcggcacg
     4201 ggcgtcacgg gtatggacga taaccggctt gttcagttca cgaccaatct ggatatgatg
     4261 gatgaaggac tcttgctgac gtactttagt ttccggtgtg taataataat ccagcccggt
     4321 ttcacccagc gctacaacac cctcttctgc cgccagacgg cgtaaatctt ctacatcgta
     4381 gggatcattc tggttgagtg gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc
     4441 taccagatcc cgcatatgta aataacccgg taatgttgtg gcgactgcca gacaaaattt
     4501 cacatcgcgt gcggcggctt tcgccagaac gtcatccacg tccttatgca aagattcata
     4561 atccagacca tcgagatggc agtgtgagtc gactaaaaac atgatgtctc tcttaaagat
     4621 gaggaaccgg tagcacaacg cccggttgca ggtaatgctc aatacgcagt aaaagatcgg
     4681 tgatgagaag ctcgcggttg atgcctgtga cagacattaa ctgttcacga atgtggcaaa
     4741 catcccccag tatagcctgc aggcgcgagg gagaaagatg gtttgccagt tcggcgacca
     4801 ggcccggcac atcaacattg gtcacctgtg cagcgccatg atggcgtttt agcgcatcca
     4861 tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg ttcatgatta agggccgcca
     4921 gcagcgaata ccagtcgccc gatggtacgc tatatgccaa cgcctgacac aatgtttcac
     4981 gagcctgcca gttatctccc tgaaacaacg ccagtgccgc gctaggcgaa ccggcactta
     5041 agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca
     5101 cggcgtactg ttccggcggc ggcgcaaggt aatgtaaccg acaacgacta cgtaatgttg
     5161 ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct ggtggctctt
     5221 caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca tcggttaccc
     5281 agacaacttt cgcaccacct aggcgtgcgt gctcattcag cttttcggtg acctcacgta
     5341 ccgcatcaat gcccagcgta tttttccctt tttcgggagc cagggtgtag taatcgggat
     5401 gcgtgccagc ctgcatcaac tgacatccac gacagtgacc gcaacttttg tggccctgcg
     5461 gttgttggca gagtaaataa cggctcaggg cgtagattaa agcatcatcg cccatgcccg
     5521 gtaacgcctg aatgagtagc gcatggtgac ctcttccggc ctgatagctg gctaccagtt
     5581 tttcgaaatc aggtcgtaac catggatacc atctcatgcg tccaactcct tcacccagtg
     5641 ggtcacggta gtgcggattg catccatcac ggcctccagc ggctgggtgg catcaatggt
     5701 atgaatactt ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc gattaaagaa
     5761 atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc
     5821 aacttccggg gtaacatcga gatagagcgt taagtcaggg cgaaaatccc cgagaacagc
     5881 atcacgcagt gttgccagca tatgttggtc aataccacgt cctccgccct gatacgcctg
     5941 agtggagaga tcgtggcgat cgccaatcac ccaggtgccg ttagccagag ctggtttgat
     6001 gaccgtttct accagttgaa cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt
     6061 aatgacttca tcgcctaccg atttgatatc cagcaccagg cttcttaact tttcggcaag
     6121 ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt
     6181 ctcaaccacc acattacgcg cggtagtttt gcctgcgcct tccagcccct caatgacgat
     6241 atacttactg cgcatttttt tccttaagca ctttcagata atcctgcaca gacttgttat
     6301 ggctggcaag attggtatta aacgtgtgac cacctttacc atcggccaca aaatagagat
     6361 acggcgtttt tgccggatgc gcagcagcct tcagcgaatc cgcccccggc gtagctatcg
     6421 cacctggcgg caaaccggta atggtatagg tgttatacgc tgtcggcgtt tccaggtctg
     6481 cacgagaaag tttgccatta taacgctctc ccatcccgta aatcacggtt gggtcggtct
     6541 gcaggcgcat accaatgcgt aaacggttga taaataccga ggcaacctga tcgcgttcac
     6601 tggcaacggc ggtttctttt tcgataattg atgccatcgt caccagctgg tttttatctt
     6661 tataaggcag gccgtccgca cgcccttccc aggcgctatc gaccgctttc accattttct
     6721 tgtgcgctcg cttgagtaac gcgacatcgg tggtattggc ggtatacatc caggtgtctg
     6781 gccagaacca accttcaatc cactccgggt tttccagttc aagtgcctgc gctacggtgg
     6841 cgtacttatc gtcactcagc gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt
     6901 agtcgctcag acgcatccct tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt
     6961 ccagcaattt cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt
     7021 taaagtgaga aagatccggt tcgatacgca gcaaccattg aaaaacccgt gggcgattga
     7081 tgatcttatc ggcataaagc tgttcaccaa gcgccagacg tccggtccct ggcttcaggg
     7141 taaatatcgt ctcttctttg ataagcaatt tgctgtcggc aagatggcga accttccaga
     7201 cgcccacacc agcggcgata cccagtacca ccagcaataa caagataatc aataacactt
     7261 ttttcatgac taattcgggc gctcacaaag tggggctaaa tattcatata acgttgctga
     7321 cgaaaaggag acatcgccac aggcacgtac gggcatcact ggcattaacg cattacaaat
     7381 aaccatctca tctgcctgca acgcctcttc cagagaggct tgcacttcga caagctgata
     7441 agaggattgt gccagcaaac ggatacagaa ttgtcgcata atgccgttaa cacctgcctg
     7501 atccagtcgc ggcgtataaa ctacattgcc cttccgccag aacaaattag ccgcacagca
     7561 ttccgtaacc cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag
     7621 atgagagcgg atcaatactt gctcaagccg attaagatgt ttaatacctg caagatgggg
     7681 attgcgcccc agccgcaccg ggcttagcgc caacgtcatc ccttcgttac gcaaacggtc
     7741 gtaatgtgca ggataaaccg taacggagag aatccgcgtt gccggtccac tgttcaatgt
     7801 gctgtaccct cgcccgccac taccgcgact gatcacgact ttcagcacac cattttgctg
     7861 ttctgctgcc agcgttttca tctcttgttc aagctgaggc cagaagtcac agaaaatcat
     7921 caaccgctga caagcatcct gtagtcgctg gatatgcgcc gataacaaac tgactttacc
     7981 gtcgataact ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc gatcgcttac
     8041 tgccagcgat tcctgcttat gaccgttaat taagaacata ccggctcctt atcagagaat
     8101 tgttagtgtg gcagcatgct cactacggaa caagtcggaa taaaagctaa gaaaaaaggc
     8161 ccgcaagcgg accttttata tgggtgggaa atgacaactt agatcttttt aaagatcaaa
     8221 gaaccattag tgccaccgaa gccgaaggag ttacacagcg tgtattccat tccgctgacc
     8281 tgacgtgctt catgcggtac gaaatccaga tcgcaacctt catccaggtt atccaggttg
     8341 atggttggcg gaacagcctg atcgcgcagc gccaggatgg agtagataga ctctactgcc
     8401 cccgccgcac ctaacaggtg accggtcata gatttcgtgg agcttaccaa cacacggctt
     8461 gcagcttcac cgaagatggc tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta
     8521 gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt caatgcctgc atcacgcaga
     8581 gcatttgcca ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt catatgataa
     8641 gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc
     8701 gcgtgttcgt actcttctag taccagcata ccggcaccat cgcccagtac gaagccatca
     8761 cgctctttat cccacgggcg gctcgccgct tgcgggttat cattgcgggt agataatgca
     8821 cgtgccgcgc caaaaccacc aacgcccagc ggcgtactgg ctttctctgc gccacctgca
     8881 accatcacgt cagcatcgcc atacgcgata atacgcgcag catgaccaat gttgtgcacg
     8941 ccggaagtac aggcggtcgc gatagagatg cttgggccac gcaggccata catgatagtc
     9001 agatgacctg ccaccatgtt cacaatcgtt gacggaacga agaatgggct gatcttacgt
     9061 ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg
     9121 gagccaattg cggcaccaat gcgggttgcg ttctcttccg ttatttcaag gccagaatcc
     9181 tgcatggcct gaacgccagc gacaattcca tattgaatga aggcatccat cttgcgctgt
     9241 tctttgcgcg agataatgtc ctcacagtta aaatccttta ctaagccagc aaatttcgtt
     9301 gcataggcgc tagtatcgaa atggtcgatt aggctgatgc cactctgacc ggcaagcaga
     9361 gctttccagg tagactctac ggtattgccg acaggagaca acatgcccag tccggtcaca
     9421 actacacgac gcttagacac gtttgtcctc cagggaggga aaaaatgatt ctagtgggac
     9481 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
     9541 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
     9601 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
     9661 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
     9721 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
     9781 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
     9841 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
     9901 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
     9961 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    10021 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    10081 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    10141 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    10201 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    10261 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    10321 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    10381 ttggtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    10441 gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc
    10501 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttagca
    10561 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    10621 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    10681 accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct
    10741 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    10801 aggccagtta gcactttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    10861 atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca
    10921 cgaatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac aggaactgtt
    10981 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    11041 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gcccgcagct
    11101 ttacaggcag cgccagcacg ctcaaccgcc tctttatgac cggcaataac cacctgtccc
    11161 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    11221 gcaatagacg catcatccag accgatgatt gccgccatag cgcccgtgcc ttctggtacg
    11281 gcttcttgca tgaacttgcc gcgcatctca accagacgca ccgcatcagc gaaatcaatc
    11341 acgccagcgc aaaccaacgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    11401 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    11461 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    11521 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    11581 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    11641 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaggtgaat
    11701 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    11761 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccaacgtc
    11821 accacgacat tgtccataga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    11881 ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt cgccgccagc
    11941 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    12001 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    12061 tcaccataac taccgtcggc atgcagatgg gtggagatga ttcccggctc ttcagaggca
    12121 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    12181 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    12241 ttcacgtatt gattggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg
    12301 tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg
    12361 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    12421 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    12481 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    12541 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    12601 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    12661 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    12721 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    12781 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    12841 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    12901 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    12961 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    13021 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    13081 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    13141 gattgtttta agcaccgctg aggcatcccg aatactgtcg agacccttta cttcttcttc
    13201 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    13261 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    13321 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    13381 ctcaatcccc tccaggggcc tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    13441 accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat
    13501 tgaactccca cgactagcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    13561 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    13621 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    13681 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    13741 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    13801 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    13861 ccgcggtagt aaccgtcggc agtgatatgg tgacgcaggt gtttttcacc agaagttttg
    13921 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    13981 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    14041 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    14101 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    14161 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    14221 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    14281 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    14341 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    14401 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    14461 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    14521 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt
    14581 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    14641 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    14701 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    14761 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    14821 gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca gaggtcgacg
    14881 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    14941 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    15001 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    15061 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    15121 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctgtgcgagg
    15181 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    15241 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    15301 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    15361 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc
    15421 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    15481 tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    15541 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    15601 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    15661 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    15721 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    15781 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    15841 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgactgcgc
    15901 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    15961 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    16021 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    16081 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    16141 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    16201 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    16261 agtttatatt caggcttaat acgttttttg ttcacccgca cttcgccttt acgcaaaata
    16321 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt gtcgatacgt
    16381 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    16441 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    16501 gttttacacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    16561 cagcaagaag tgaaaaatgt aatgaacgca tcccacacaa ttaaacacgg agcagtatga
    16621 tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag atttccggca tcatagtgcc
    16681 caatacggga tgcataaagc tccctcagac tggagactcc acaaatgaaa tatacaccgg
    16741 ttggcgttg
//
LOCUS       DAJVOY010000083        16557 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVOY010000083 DAJVOY010000000
VERSION     DAJVOY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.083"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            4..1104
                     /gene="arnB"
                     /locus_tag="OQL90_002366"
     CDS             4..1104
                     /gene="arnB"
                     /locus_tag="OQL90_002366"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1369118.1"
                     /translation="MEELAAVKEVLESGWITTGPKNQALEQAFCQLTGNQHAIAVSSA
                     TAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDVDRDTLMVTPEAIE
                     SAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVGTYYKGRHIGAKGT
                     AIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDRQTWGRAPQAEVLT
                     PGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPFQPLSLPAWPHVHA
                     WHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYRERFPTLSLPNTEW
                     NSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1108..2076
                     /gene="arnC"
                     /locus_tag="OQL90_002367"
     CDS             1108..2076
                     /gene="arnC"
                     /locus_tag="OQL90_002367"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1369119.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2076..4058
                     /gene="arnA"
                     /locus_tag="OQL90_002368"
     CDS             2076..4058
                     /gene="arnA"
                     /locus_tag="OQL90_002368"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS1369120.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSAISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4055..4945
                     /locus_tag="OQL90_002369"
     CDS             4055..4945
                     /locus_tag="OQL90_002369"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1369121.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            4945..6597
                     /gene="arnT"
                     /locus_tag="OQL90_002370"
     CDS             4945..6597
                     /gene="arnT"
                     /locus_tag="OQL90_002370"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1369122.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6594..6929
                     /gene="arnE"
                     /locus_tag="OQL90_002371"
     CDS             6594..6929
                     /gene="arnE"
                     /locus_tag="OQL90_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1369123.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            6929..7315
                     /gene="arnF"
                     /locus_tag="OQL90_002372"
     CDS             6929..7315
                     /gene="arnF"
                     /locus_tag="OQL90_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1369124.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7309..7575)
                     /gene="pmrD"
                     /locus_tag="OQL90_002373"
     CDS             complement(7309..7575)
                     /gene="pmrD"
                     /locus_tag="OQL90_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1369125.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7685..9040)
                     /gene="menE"
                     /locus_tag="OQL90_002374"
     CDS             complement(7685..9040)
                     /gene="menE"
                     /locus_tag="OQL90_002374"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1369126.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9037..9999)
                     /gene="menC"
                     /locus_tag="OQL90_002375"
     CDS             complement(9037..9999)
                     /gene="menC"
                     /locus_tag="OQL90_002375"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1369127.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(9999..10856)
                     /gene="menB"
                     /locus_tag="OQL90_002376"
     CDS             complement(9999..10856)
                     /gene="menB"
                     /locus_tag="OQL90_002376"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1369128.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10871..11629)
                     /gene="menH"
                     /locus_tag="OQL90_002377"
     CDS             complement(10871..11629)
                     /gene="menH"
                     /locus_tag="OQL90_002377"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1369129.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11626..13296)
                     /gene="menD"
                     /locus_tag="OQL90_002378"
     CDS             complement(11626..13296)
                     /gene="menD"
                     /locus_tag="OQL90_002378"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1369130.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13385..14680)
                     /gene="menF"
                     /locus_tag="OQL90_002379"
     CDS             complement(13385..14680)
                     /gene="menF"
                     /locus_tag="OQL90_002379"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1369131.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14759..15064)
                     /gene="elaB"
                     /locus_tag="OQL90_002380"
     CDS             complement(14759..15064)
                     /gene="elaB"
                     /locus_tag="OQL90_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1369132.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15119..15580)
                     /locus_tag="OQL90_002381"
     CDS             complement(15119..15580)
                     /locus_tag="OQL90_002381"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1369133.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15645..>16557
                     /gene="rbn"
                     /locus_tag="OQL90_002382"
     CDS             15645..>16557
                     /gene="rbn"
                     /locus_tag="OQL90_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS1369134.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFN"
BASE COUNT         3818 a         4506 c         4256 g         3977 t
ORIGIN      
        1 ggcgtggagg aactcgctgc agttaaagaa gttctcgaat ccggttggat cacaaccggt
       61 ccgaagaatc aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc
      121 gcggtcagtt cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag
      181 ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg
      241 ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa
      301 gcgatcgagt cagccattac gccacgcact aaagccatca ttccggtgca ttatgccggt
      361 gcgccagcag atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc
      421 gaagatgctg cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt
      481 accgctattt tttcatttca tgccattaaa aatattacct gtgcggaagg tggcctgatt
      541 gtaactgata atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggcctgggt
      601 gtcgatgcct acgacagaca aacctggggc cgagcaccgc aggctgaagt cttaacaccg
      661 ggctataagt acaatctgac cgatattaac gccgcaattg ccctgacaca gttagcaaaa
      721 ttagagcacc tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca
      781 gctctcccct ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg
      841 tttattattc gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg
      901 ttaaaagaaa gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat
      961 tatcgcgagc gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc
     1021 tgttctttgc cgctgttccc ggatatgact accgccgatg ccgaccgtgt catcacagcc
     1081 cttcagcagc tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg
     1141 gtggttattc ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc
     1201 acagcctgtg aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc
     1261 gataattccg cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct
     1321 attttgctta accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt
     1381 actggcgact taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc
     1441 cgcctagtgg caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc
     1501 caggacagct ggtttcgtaa aacagcttcg aagatgatta accggcttat tcagcgcacc
     1561 accggcaaag cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc
     1621 gatgcgatgt tgcactgcca tgaacgtagc acctttatcc cgattctggc gaatatcttc
     1681 gcccgccgtg ccattgaaat tccagtacat catgccgagc gtgagtttgg tgaatccaaa
     1741 tacagtttta tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg
     1801 ccgctacgta tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg
     1861 gtgctgctgg tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt
     1921 atgctatttg ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc
     1981 ggtgaatata tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag
     2041 caagttatcc gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc
     2101 acgatatggg atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca
     2161 tttttaccca taccgataat cccggtgaaa aagcctttta tggttcggtg gctcatctgg
     2221 cggcggaaag aggcattccg gtttatgcgc cggataacgt taatcatccg ctgtgggtgg
     2281 aacgcattgc ccaactgtca ccagaggtga ttttctcttt ttattatcgc catcttattt
     2341 gcgacgagat tttgcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac
     2401 caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaacgg
     2461 gcgttacatt gcaccgaatg gtaaaacgtg ccgatgctgg ggccattgtg gcgcaactgc
     2521 gcgttgccat tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgcca
     2581 cgcgccagct actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg
     2641 cccagcgcga aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg
     2701 aatggcataa accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc
     2761 cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc
     2821 ctcatgccag caaagcacag ccggggagcg cgatttctat tgcaccgctg ttgattgcct
     2881 gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg
     2941 gctcgcaatt agcgcagacg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg
     3001 cctgcaccgc ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca
     3061 accatctgac agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg
     3121 gcagcgatgc gataagccgt tttctgaatc atccgcattt tcactttgtt gaaggcgata
     3181 tcagtattca ttccgaatgg attgagtatc atgtcaaaaa atgtgatgtc gtcttgccgc
     3241 tggtggcgat agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg
     3301 attttgaaga gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct
     3361 tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt
     3421 ctaatttaat cgtcggcccg gtgaataaac cacgctggat ttattcggta tcaaaacaat
     3481 tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc
     3541 gcccgtttaa ctggatggga ccacgactgg ataaccttaa tgcggcacga atcggcagct
     3601 cccgcgctat tacgcaactc attctcaatc tggtagaagg ttcaccgatt aagctgattg
     3661 atggcggaaa acaaaaacgc tgctttactg atattcgcga tggtatcgag gcgttatacc
     3721 gcattatcga aaatgcggga aatcgctgcg acggtgaaat tatcaacatt ggcaatcctg
     3781 agaacgaagc gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc
     3841 cgctgcgcca tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agcagctact
     3901 acggcaaagg atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct
     3961 gcctggactg ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct
     4021 tcctgcgcac cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga
     4081 tgtcgatacc tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa
     4141 gcataatatt caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct
     4201 ctggcgactg gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct
     4261 ttatggctgg gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc
     4321 caatgccgat atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga
     4381 tcaccatgcc tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat
     4441 tgcacgcggt cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc
     4501 tgcgggctgg cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta
     4561 caacagcgat tgtcgtgggg ccatgccgtt ccgtccattg ctcgaatcag gaaaccctgg
     4621 cactgcgcaa attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa
     4681 aacagaagat tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt
     4741 ttataccatt catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct
     4801 caaacgcgca gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac
     4861 gttgccgctc ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg
     4921 ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg
     4981 tttattgcct gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa
     5041 acgcgttatg cggaaatcag tcgagaaatg ctagcatccg gcgactggat tgtgccccat
     5101 ctgttagggc tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg
     5161 caatggctat ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg
     5221 ttaactgccg cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct
     5281 ctactcgcca cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc
     5341 gtgctcgatc cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca
     5401 atgcaggcac agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc
     5461 ggcatggggg tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg
     5521 ccatgggtag cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt
     5581 atcagttgcg tactgacggt tctcccttgg ggactggcga tagcgcagcg ggagcctgac
     5641 ttctggcact attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa
     5701 catagagctc cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg
     5761 ggattactcc ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc
     5821 tatttgttga gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg
     5881 cccacctata ttctttcctg ctttgcatct ctggcaatgc tgatggcgca ttacgctttg
     5941 ctggcagcaa aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt
     6001 ggcgtcactg gcattattgc cacatttgtg gtctccccgt ggggaccaat gaacacgccg
     6061 gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg
     6121 gcatttttcg gctggtacac cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt
     6181 tgcccgctgg ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga
     6241 aaacatccgc aattttttgt cgagatgaca caagaatcac ttcaaccaag ccgctatatt
     6301 cttactgata gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc
     6361 atcatgtatc gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg
     6421 agatttgtca gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt
     6481 attacactcg tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc
     6541 gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct
     6601 ggctaacatt agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg
     6661 caacctgctt tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg
     6721 cgctggcttg tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg
     6781 taggcattgc ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa
     6841 aactgtggca cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg
     6901 gcattgtgat cctcgggagt acggtgtaat gggcctgata tggggattat tcagcgtcat
     6961 tattgcctca gttgcgcaat taagcctggg ttttgcggca agtcatctgc cgccgatgac
     7021 gcacttatgg gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt
     7081 actcgggctg ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact
     7141 tgcgctaagc aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc
     7201 aatggtactt cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg
     7261 tattatgagc gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt
     7321 ccctgccgct gtacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac
     7381 tttcaccgta tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag
     7441 taaatccccc actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc
     7501 gccagcatcg cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt
     7561 gaccagccat tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt
     7621 aacagataaa ctctttatca acggcaatgg aatggcagaa atgagaagtg aaacggaggg
     7681 ttaattattg ctgacgttgc acccactctt ttagcgcctg acgtgaaatt ttaataccgc
     7741 cgtttttcag ctccggcggt aaagtcagcc agcgcaccgg ttgttgaaaa cgagccagct
     7801 tatctttcac ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg
     7861 gccgatgacc aaactccttg tcggcaacgg gaacgataaa cacctgcaaa accgcaggat
     7921 gtgcagcaat aacacgctcg acttcttccg gctgaatacc ctcaccgcca ctgaataaca
     7981 gattgtctaa acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg
     8041 tagcgtacca gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt
     8101 ctgccatact ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg
     8161 gcagcggcga accaacgtct gccaggccgt cggcttcttt cgcacatacc gtggaggcaa
     8221 actcggtcag accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca
     8281 actcgaccgg gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt
     8341 taaccagcaa acgccagagt tgtgttggca ccagtgaagc gtgagtacag cctgccagca
     8401 tttgctccaa tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc
     8461 acataattcc ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat
     8521 cgccaaacgg aatcagcgat aacacacctt gcgcactggc aagatgggcc tgataagtat
     8581 ggacagcggc tttcggtaaa ccggtggagc cggaggtcaa tgtcattgaa caaagacgtg
     8641 ttggttgcca cagagctgaa tgtgtgcctt caaccatctg aatatgcagt gaagttaacg
     8701 ccgggaaagt attttctcct tccagcacca gcgcatattg cagtgtaaga tttggcagta
     8761 attcctcaag caacggttgc ggaagctgtg gatttacggg cagcacccgc gccccgcatt
     8821 gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc
     8881 cgctgccctc aaccacaccc tgcgccgcaa agcccgtagc taactcatcg acgcgagcac
     8941 aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca
     9001 cttgccgcca gtgacgccac ggccagtcag agaagatcat aacaaccgct ccagtgcatc
     9061 aacttccacg acaggcagcg tgctacccgg ccagcgacgt acctgctgcg cctgcatcag
     9121 atccagcgtg tccagccctg gaatggtgtc cggcgttaac caggcggcaa tccgcgccag
     9181 ttgcgttaag cctaagctcg actcaataga agaactgatc accgccgtca gcctcaacgc
     9241 gtgcgccgcc tgtacctgct cgcgtacttt ttccagactg cccgtgagcg tgggtttgat
     9301 aactaccgcg cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaggctttc
     9361 atcccaggca atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt
     9421 gcacggctct tcgagaaacg cgatgcggtg gcgataatcc gggttaacat atttggcaaa
     9481 ctgctgacct ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg
     9541 aattgcctcc agcaacagat taaccaccat gccgtcgcgc acggcttcgt acaatcccac
     9601 tttgaccttc gccactttct cgcctggcat atcagcaagt ttgaggatca gatcgtcagg
     9661 atcgccatta cacagtggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag
     9721 ctccgccaat gcacagctta cgccaaaggc aacggaaggc atctgcagta gctcgcaatc
     9781 gcctgccagc cagttattta cccaggcaag cagcacgctt tgcgcctctt cccaggtttc
     9841 ctggctgaag cccggcagtg gggagatctc cccccaccct tcgcgctcgc cctcacgcag
     9901 gcaaacgtac agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc
     9961 catggggatc tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg
    10021 ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc
    10081 atgtagaaca gcatggtggc gttgcccgcc agctcctgca gccccgcctg cccgtcacag
    10141 tcggcgttca gtgcagcttt caggcagcgc agcgccatcg gactgttttg cagcatttcg
    10201 cggcaccagc gaacggtctc tttttccaga tccgccagcg gtaccacggt gttgacaagg
    10261 cccatatcca gcgcctgttt cgcgtcgtac tgacggcaca ggaaccagat ttcccgcgct
    10321 tttttctgtc cgacgatgcg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg
    10381 actttcgggc cagtctgacc gaagatggca ttatctgccg cgatagtcag gtcgcacatc
    10441 atgtgcagaa cgtgaccgcc gccgatggag tagccagcca ccatcgcgac aaccggtttc
    10501 ggacaggtac ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca
    10561 tctttatagc cgccgtaatc accacgcact ttctggtcac cgccggagca gaacgcttta
    10621 tcacctgcgc cagtcagaat gatcacgccg acgttgtcgt cataacgcgc atctgccagt
    10681 gcctggatca tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta
    10741 atggtgattt ttgcgatacc atcggtggat ttttcataac gaatgtcctc gaaaccttcg
    10801 gagcagtcgt gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg
    10861 tgtcctttag tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt
    10921 cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg
    10981 ccagggcgcg gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg
    11041 cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg
    11101 caagggttgc gccattattg ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat
    11161 tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg
    11221 tgcgaaaacg ctgcgcccat tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat
    11281 tttgcagccc cggatgcccg ccttcgacaa taaccccaca aagccccgcc agcccctggc
    11341 aagccgccat catcgccacc cgtccgccaa gcgagtaccc caccagccag aagtcaagga
    11401 tgttgtaact aaccaaggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc
    11461 taatcgccgc cgaaccacca tgacctggga gatcaacata caaccgtgag tagtcggcaa
    11521 acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca
    11581 gccagggtaa acctggtttt ccgtgttttg cctgcgcgtg caggatcata aatggcttac
    11641 ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat
    11701 caccgtggtg gttggcgtgc gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt
    11761 ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg
    11821 cggcatcaga tagaaacgct cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg
    11881 cccgccgttg ttgttcacca caattaatac cagcggcgca gaaacctgac gcaataacgc
    11941 cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc
    12001 gcttgcccgc tgaacgcctg cggcggtcga aagcagtccg tcgataccgc tggcccctcg
    12061 gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac
    12121 caggctgtta ccgacaaaca attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc
    12181 cagttgcgct tcgccaaacg catcgcgacg ggcaataacc gcctgcattg cctgttccgc
    12241 caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca
    12301 gtcggcaata ttggcaatta agcggcgtcc acgatggtgc gccggatcaa gccgcccttc
    12361 gatgtcatcc acaatccagt actcttcggg ttcacagctt gcctgccatt gcaggagccg
    12421 tttgcccgtc aggctgcttc ccagttgcac cacaatttgc gcctgctgca gctcgctggt
    12481 cgctttggca ttgcctaacc aaagatcggc acacggcagc ggctgcccgg tttgtgacag
    12541 cacatcgcca atcagcggcc agccaagagt ttgcgcccac agggcaactt ttttgccctc
    12601 ttccgcactc atgcgcccgg caaccaccac gccgcgcttt tgtcgccaga agaaccagtc
    12661 gcgctgtttt tcactttcca gactaggcgc ttcacgcagc cacggtttgt cgtcctgcca
    12721 ccagtcaccc agacgctgtt gccagctaag cccggtatcg tccatttcgc catacagcgg
    12781 ttcagcaaac gggcagttga tatggactcc ccccgcatga agcgtaccga gagcgtggtc
    12841 gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca atgaaatact
    12901 gtgcgtgggg tgagaggcga acattcccgg ctggcgaatt gcctgattcg cgccgcagtc
    12961 aattagctcc ggcgggcgat cggcggttaa gagaatcagt ttttctccgg ttaacccggc
    13021 ttcaatcagt gccggataga gatttgccac cgccgtgccg gaggtcacaa tcaccgccac
    13081 cggctgcttg ctgactttcg ccagccccag cgccagatgc cccaacccac gctcatcgaa
    13141 atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg
    13201 cgagcctggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac
    13261 cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc
    13321 ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc
    13381 attactattc catttgtaat aaagtacgca accctgccgc tttgttgtcg atttcctgcc
    13441 actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat
    13501 tgccgctaat ttttgctgag cgcagggaaa cgcagaattc gctttgttgt aatgagagat
    13561 agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc
    13621 gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga
    13681 tcacatcatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac
    13741 gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt
    13801 ggcagatatc ttccaccacc agcatgttct cgcgctggtt tttatcatcc gccatcagcc
    13861 actcttctaa ctgctgcgcc tgcttatcat caggatgatt tgctactgtt cccgccagcg
    13921 cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa
    13981 aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac
    14041 tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag
    14101 cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt
    14161 gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag
    14221 gcaagggctt gatactcacc agtgtggcga taaattcttt tgcctgaatc gcatcgtgct
    14281 gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc
    14341 attccaggcg gggtaaaagt aaattgccct gcgacgggtc aaaagcattc agcccccaaa
    14401 tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg
    14461 taaaacgggt aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt
    14521 agaattgcgg gtacgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga
    14581 gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat
    14641 ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa
    14701 agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt
    14761 aacggcgtgc cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt
    14821 gccaaggttt ttcgtggacg tagtcatcag cacgataaac agcctgcttc gcccgatagt
    14881 aataactgtc tgaaacctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac
    14941 gcgctttcag ctcaacatat ttctgatcag cgggatcgcc agaggagcgg agcacctctt
    15001 ccagtgtctc actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag
    15061 acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt
    15121 acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct
    15181 ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga
    15241 ggtaaacagg cttatcaggc cagtgatgcg tgcaggtgtc gagggtttta ctcatcagtt
    15301 gctgccccac tttttcgcca cgcaacgctt cactgacaat cacccgacca ataacgaccg
    15361 gctcaagatc atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc
    15421 aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc
    15481 aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa
    15541 ccgataattc agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt
    15601 ataccttata ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt
    15661 taggtacttc agccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc
    15721 tgcaacatcc gacccagagc ggactttggt tgtttgactg cggtgaaggc acccagcatc
    15781 agctactgca taccgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc
    15841 atggcgatca tctttttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta
    15901 tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc
    15961 ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa
    16021 ttctcgatga tggcctgcgc aaagtaaccg cttatccgct ggaacaccca ctggaatgtt
    16081 atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag
    16141 ctgctggcgt gccacctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc
    16201 tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag
    16261 cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg
    16321 tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc
    16381 gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggtaagc
    16441 taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg
    16501 aatgcaggtc aattttcccg gcgactgaac tagcgaatga tttcaccgtg tttaacg
//
LOCUS       DAJVOY010000084        16522 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVOY010000084 DAJVOY010000000
VERSION     DAJVOY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.084"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            95..2323
                     /gene="crfC"
                     /locus_tag="OQL90_002383"
     CDS             95..2323
                     /gene="crfC"
                     /locus_tag="OQL90_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1369135.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2320..3203
                     /locus_tag="OQL90_002384"
                     /pseudo
     CDS             2320..3203
                     /locus_tag="OQL90_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            3467..4969
                     /gene="proP"
                     /locus_tag="OQL90_002385"
     CDS             3467..4969
                     /gene="proP"
                     /locus_tag="OQL90_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1369136.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5035..5172
                     /gene="pmrR"
                     /locus_tag="OQL90_002386"
     CDS             5035..5172
                     /gene="pmrR"
                     /locus_tag="OQL90_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1369137.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(5148..6248)
                     /gene="pmrB"
                     /locus_tag="OQL90_002387"
     CDS             complement(5148..6248)
                     /gene="pmrB"
                     /locus_tag="OQL90_002387"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1369138.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6249..6917)
                     /gene="basR"
                     /locus_tag="OQL90_002388"
     CDS             complement(6249..6917)
                     /gene="basR"
                     /locus_tag="OQL90_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1369139.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6914..8557)
                     /gene="eptA"
                     /locus_tag="OQL90_002389"
     CDS             complement(6914..8557)
                     /gene="eptA"
                     /locus_tag="OQL90_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1369140.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8661..9998)
                     /gene="adiC"
                     /locus_tag="OQL90_002390"
     CDS             complement(8661..9998)
                     /gene="adiC"
                     /locus_tag="OQL90_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1369141.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10135..10896)
                     /gene="adiY"
                     /locus_tag="OQL90_002391"
     CDS             complement(10135..10896)
                     /gene="adiY"
                     /locus_tag="OQL90_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1369142.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11221..13488)
                     /gene="adiA"
                     /locus_tag="OQL90_002392"
     CDS             complement(11221..13488)
                     /gene="adiA"
                     /locus_tag="OQL90_002392"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1369143.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13687..14595)
                     /gene="melR"
                     /locus_tag="OQL90_002393"
     CDS             complement(13687..14595)
                     /gene="melR"
                     /locus_tag="OQL90_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1369144.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14878..16233
                     /gene="melA"
                     /locus_tag="OQL90_002394"
     CDS             14878..16233
                     /gene="melA"
                     /locus_tag="OQL90_002394"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1369145.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16336..>16522
                     /locus_tag="OQL90_002395"
     CDS             16336..>16522
                     /locus_tag="OQL90_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1369146.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIM"
BASE COUNT         3987 a         4001 c         4349 g         4185 t
ORIGIN      
        1 tatttcttgc agtaattata aaagccgaaa acagaactca aaaacaatct ggctaaataa
       61 aagtaacaaa atttgcttta aggaagaatt ttctatgtac acacagaccc tgtatgagtt
      121 aagtcaggag gctgaacgcc tgttacagct ttctcgccaa cagttgcagt tactggaaaa
      181 aatgcctctc tctgtacccg gagacgatgc ggcacaactg gctttaccct ggagtcagcc
      241 taatatcgcc gaacgttacg cgatgctgaa taatgagttg cgtaaaattt cccgactgga
      301 aatggtgctg gcgattgtcg gtactatgaa agcagggaaa tcaaccacca ttaatgccat
      361 tgttggtacg gaggttctgc ctaatcgtaa tcgcccaatg actgcgctgc cgacgcttat
      421 tcgccatacg cccgggcaaa aggaaccggt actgcatttt tcacatgtcg cgccaatcga
      481 ttgtttaatt caacaattac aacagcgcct gcgtgattgc gatattaagc atctgaccga
      541 tgtgctggaa atagataaag atatgcgtgc gcttatgcag cggatcgaaa atggcgtcgc
      601 tttcgaaaaa tattatctgg gtgcccagcc tatttttcat tgtctgaaaa gtttgaatga
      661 tttagtgcga ctggcgaagg cgctggacgt cgattttcct ttttctgctt acgccgccat
      721 tgagcatatt cccgtgattg aagtggagtt tgtccatctg gcggggctgg aaagttatcc
      781 cggtcagttg acgttactgg atacccccgg gccaaatgaa gccgggcaac cgcatctgca
      841 aaaaatgctt aaccagcagc tggcacgcgc ctcggcggta ctggcggtgc tggattatac
      901 gcaactgaaa tcgatctccg atgaagaggt ccgtgaggcg attttggcgg tggggcaatc
      961 ggtgccgctg tatgtgctgg tcaataagtt cgatcaacag gatcgtaaca gtgacgacgc
     1021 cgaccaggtg cgggcactga tttccgggac gctgatgaaa ggctgtatta cgccacagca
     1081 gatatttccg gtgtcgtcga tgtggggcta cctggcgaat cgggcgcgct atgagttagc
     1141 caacaacggt aagttaccag cgccagagca acaacgctgg gtggaagatt ttgcccatgc
     1201 cgcgctcggc aggcgctggc gtcatgccga tctggcggac ctcgaacata ttcgtcatgc
     1261 tgccgatcag ttgtgggaag attcgctgtt cgcccagcca attcaggcgt tgcttcatgc
     1321 cgcttacgct aacgcctcat tgtatgctct gcgatctgct gcgcataaac tgttgaatta
     1381 cgcgcagcag gcgcgggaat acctggattt tcgtgcgcac gggttaaacg tcgcttgtga
     1441 acaattgcgg caaaatatcc atcaggtcga agaaagtttg cagctattgc aactcaatca
     1501 ggcgcaggtg agcggcgaga ttaaacatga aatcgagctg gccctgacct ccgccaacca
     1561 ctttctgcgt caacagcaag atgcgctgaa tgcgcagtta gccgccttgt ttcaggatga
     1621 ttcggagccg ttaagcgaga ttcgtacctg ctgtgagaca ctgttacaga cggcgcagaa
     1681 caccatcagt cgcgacttta cgctgcgttt tgccgagctt gaatccaccc tttgccgggt
     1741 gttaaccgat gttattcggc ccattgagca acaagtcaaa atggaattga gcgagtcagg
     1801 gtttcgtcct gggtttcatt ttcctgtttt tcacggagta gttccccacg tcaacactcg
     1861 ccagctgttc agtgaagtca tttcgcgcca ggaggcaacg gacgagcaga gcacgcgttt
     1921 aggcgttgtg cgtgagactt tttcgcgctg gttgaatcag cccgactggg gacggggaaa
     1981 tgagaaatcc ccgacagaaa cggttgatta cagtgtgttg caacgagcat taagcgcaga
     2041 agtcgatctt tattgccaac aaatggctaa agttctggca gagcaggtcg atgaatctgt
     2101 tacggcaggc atgaatactt ttttcgctga gttcgcttca tgtttgacgg aattacagac
     2161 gcgtttacgc gaaagcctgg ctctgcgtca acaaaatgaa tcggtggtca ggctgatgca
     2221 gcagcaattg cagcagactg tgatgactca cggctggatt tacaccgacg cccagctgtt
     2281 acgcgatgat attcaaacac ttttcacggc agaacgatat tgaccaagac gttactggac
     2341 ggccccggtc gcgtgctgga gtcggtttat ccccgctttt tagtggatct ggcgcagggc
     2401 gatgatgccc gccttccaca agcccatcag cagcagtttc gtgaacgact gatgcaggaa
     2461 cttctttcgc gtgtgcagct tcagacatgg acgaacggcg gcatgttaaa tgcgccgctt
     2521 agcctgcgac tgacaatggt ggaaaaactg gcgtcgatgc tggatcccgg tcatctggcg
     2581 ctgacgcaga tcgcgcagca tctggcgctg ctgcaaaaaa tggatcaccg ccagcactct
     2641 gctttcccgg agctccccca gcaaattgcc gccttgtatg agtggttttc agcccgttgt
     2701 cgctggaagg aaaaggcgtt aacgcaacga ggccaggcct actggtgcag gcaggtgatc
     2761 agagcgagca aatttttacc cgctggcgtg ctggggcgta taacgcctgg tcgttgcctg
     2821 ggcgctgttt tatcgttctc gaagagttgc gttggggggc atttggcgat gcctgtcgtc
     2881 tgggaagccc gcaagcggtg gcgttgctgc tgggtgattt gcgcgtgaaa gcgacacaac
     2941 atctggcaga gagtatcaat gcggccccga ccacgcgtca ctattaccat cagtggtttg
     3001 cctcttcgac cgttctgacg ggcggggatc atgctgattt tttaagttgg ctgggaaagt
     3061 ggaccacggc agataaacaa cccgtttgct ggtcagtgac ccaacgttgg caaactgtcg
     3121 cgctggggat gccacgactc tgttcagcgc agcgtttggt gggggcaatg gtcgaggaaa
     3181 tcttctctgt aaatttggcg taaataatca gttacatcaa tgagtcctaa acgaaatcca
     3241 tgtgtgaagt tgatcacaaa tttaaatact ggtagggtaa aaaggtcatt aactgcccaa
     3301 ttcaggcgtc aactggtttg attgtacatt ccttaaccgg agggtgtaag caaacccgct
     3361 acgcttgtta cagagattgc atcctgcaat tcccgctccc cttttgcggc cgtcgcgctg
     3421 atttttctgg cgtttgcgga aatgggccaa ctctgcgagg aaagctatgc tgaaaaggaa
     3481 aaaagtaaaa ccgattaccc ttcgtgatgt caccattatt aatgacagta aactgcgtaa
     3541 agccattacc gcagcatcac tgggtaatgc aatggaatgg ttcgattttg gtgtttatgg
     3601 ttttgttgct tacgcattag gtaaagtttt tttcccgggg gctgacccca gcgtgcagat
     3661 ggttgctgca cttgccactt tctccgttcc ctttctgatt cgaccgcttg gcggactctt
     3721 ctttggtatg ttgggcgata aatatggtcg ccagaagatc ctcgctatca ctattgtgat
     3781 tatgtcgatc agtacgttct gtattggctt aataccgtcc tacgacacga ttggtatttg
     3841 ggcaccgatt ctgctgttga tctgtaagat ggcacaaggt ttctcggtcg gcggtgaata
     3901 taccggggcg tcgatatttg ttgcggaata ctcccctgac cgtaaacgtg gctttatggg
     3961 cagctggctg gacttcggtt ctattgccgg gtttgtgctg ggtgcgggcg tggtggtgtt
     4021 aatttcgacc attgtcggcg aagcgaactt cctcgactgg ggctggcgta ttccgttctt
     4081 tattgctctg ccgttaggga ttatcgggct ttacctgcgc catgcgctgg aagaaactcc
     4141 ggcgttccag cagcatgttg ataaactgga acagggcgac cgcgaaggtt tgcaggatgg
     4201 cccgaaagtc tcgtttaaag agattgccac taaatactgg cgcagcctgt tgacatgtat
     4261 tggtctggta attgccacca acgtgactta ctacatgttg ctgacctata tgccgagtta
     4321 tttgtcgcat aacctgcatt actccgaaga ccacggggtg ctgattatta tcgccattat
     4381 gatcggtatg ctgtttgtcc agccggtgat gggcttgctg agtgaccgtt ttggccgtcg
     4441 tccgtttgtg ctacttggta gtgttgccct gtttgtgttg gcgatcccgg cgtttattct
     4501 gattaacagt aacgtcatcg gcctgatttt tgccgggtta ctgatgctgg cggtgatcct
     4561 taactgcttt acgggcgtta tggcttctac cttgccagcg atgttcccga cgcatatccg
     4621 ttacagcgcg ctggcggcgg catttaatat ttcggtgctg gttgccggtc tgacgccaac
     4681 actggcggcc tggctggtcg aaagctcgca gaatctgatg atgcctgcct attacctgat
     4741 ggtagtggcg gtgattggtt taatcaccgg cgtaaccatg aaagagacgg caaatcgtcc
     4801 gttgaaaggt gcgacaccgg cggcgtcaga tatacaggaa gcgaaggaaa ttctcgtcga
     4861 gcattacgat aatatcgagc agaaaatcga taatattgac cacgagattg ccgatttgca
     4921 ggcgaaacgt acccgcctgg tgcagcaaca tccgcgaatt gatgaataag ctgaaacgga
     4981 tggcctgatg tgacgctgtc ttatcaggcc aattgaactc ttaaggttca cttaatctct
     5041 gacgcgcata ctctcctcca ggttaacgga ggagagtgca atgaaaaacc gtgtttatat
     5101 gaaagtttaa ctaccgtgtt cagcgtgctg gtggtcagca gctttcttta tatctggttt
     5161 gccacgttct gatctttctt cagccgtacc caggcccgcg tgccggaagt ctcttgccgg
     5221 ttttgcagga aaaactgccc gtgatgcaac tgtgtaatgc ggctgacaat acttaacccc
     5281 agaccaatcc cgccataacg gctgtccata cgtacaaacg ctttactcaa ctccccgcat
     5341 ttactctcat caatacctgg tccttcatct tcaactgcca tgaccgctcc gccatcttct
     5401 tgcagcttaa tcataatgtt gctgccttgc gggctgtaac gatgggcgtt ttctaccagg
     5461 tttcgcaata acatccgcag cagggttgca tcaccctgaa cggtgatgtc ggcggcgctc
     5521 tctggcaata gcagggtttg ctgtcgctgg tcgagcatgg tactgagttc gtcatacgag
     5581 gggagaatga catcttccag cagttttaca tgttgataat taccggaaga aaatgactgt
     5641 ccggcacgcg ccagttgcag cagctgggag acgctctcca tcatctgatc aagccgtgcc
     5701 actaacggtg ctacatcaat gtgatgcgtt ttcgccagca gttccagatg caaacgcacc
     5761 cccgccagtg gcgttcgcag ttcgtgcgcg acgtcagcgg taaacaacct ttcgttatcc
     5821 agcgtgctgg tcaggcgact gaccagatcg ttcaacgccg aaaccaccgc ttcgatttcg
     5881 agggtggcgc tgtgaatggc aatgggcgtt aagttgtcgg cagtgcgcgc ttccagctct
     5941 ttttgcagct ccgccagcgg gcgggtgatg cggcgtaccg cctgatagca gataaatagc
     6001 gtcaggctga ccataaagac gccggggaca atcaggctgg cgaccgcctc gcggatctca
     6061 cgcatgatgt ggcgatcgtt gttgcgattg tcgcgtagcg cctgctcaaa cagctgaatc
     6121 tgctcggtac tttcatgcca tagccagaag acgctgatca gctcaaacac caacaaaatg
     6181 gccccgatag tcaatatcag ccgttggcgc agcgatattg gtcggcgcag aaaacgcatc
     6241 agattcaatt agttttcctc attcgcgacc agcatatagc caaagccgcg cacggtgcgg
     6301 atacgggatt tgcccacttt gtcgcgcaga ttgtggatat gcacttccag ggtgttggtc
     6361 gagggttcat tgtcccagtt atagatgtcg ttgtagagaa tttcccgatg taccggactg
     6421 cctgctttga gcattaaccg tgacagcaga gcatattctt tgggcgtcag aatcaactct
     6481 tcaccgccca tccatacctg acggcgaccc atattcagcg tcagattgcc aacaatcagc
     6541 tcactttcgc cctgattatt atggcgtcgt agcagggcgc ggatacgggc gtgtaactct
     6601 tccagcgcaa aaggcttcac cagatagtca tcggcaccga catccagccc ggcgattttg
     6661 tcggtcagcg tatcgcgagc ggtgaggatc agtaccggca gggtatattt tttctgccgg
     6721 atacgggcga gaaaatgcag tccatcttcg tcgggtaacc ctaaatccag taccaccagg
     6781 ctgtaatgac cggcttcaag gctttgttcc gccatccgcg cggttgtcac gccatcgcac
     6841 gcgtagcctt cggtttgcgc cgccagaatc agtccctgca ataacagcgt atcgtcttca
     6901 acaatcagaa ttttcattta ctcactctcc tgcaagtttg cagaatatca tccgcagcct
     6961 ggtaatactt cgtctcaacg ccagttaatc ccaatagcgt ggagaataaa ttgtcttgtg
     7021 aatagtgttg cgtttgcgcc tgtttttgca ggcagttctg gtcaacctga taccgttttt
     7081 gataatcctc cgacagccac agcagcatcg gcacctgttt ttggctatcc ggggcgatgg
     7141 cataaggcag accgtgcaga tagatgccat tttcacctaa cgattcaccg tggtcagaaa
     7201 gataaaccag gctggtggta aatttatcct gatgttcttt cagcagatta atcgctttat
     7261 caacaatata gtcgacgtaa accagcgtgt tgtcgtaagt gttcaccagt tgctctttgg
     7321 tacaggtctg gatctcattg gtgtcgcagg ttggggtaaa tttcctgaac tgcggcggat
     7381 agcggttgta ataggtcgga ccgtggctgc cgatggtgtg taagacaatc acgccatcac
     7441 cctgcaggtt attgatgtac tcttccagcc cgtggaacag cacttcgtca tagcattcgc
     7501 cgttgatgca ctgaccaggc agattcagcg cggtgacgtt ctggtgaggc acgcggtcgc
     7561 aggcaccttt acagccgcca tcgttgtcat tccacagcac gttgatgccc gctcgctgaa
     7621 tgatatccag cacgccttcc tggtgctgtg ccagctcttc tttgtaatgc tcacgcggca
     7681 tatccgagaa catgcacggt actgaaactg ccgttgccgt gccgcaagat acggtattag
     7741 ggaaatagac cacgttatct ttcgccagcc gcgggttagt ttcacgcggg tagccgttga
     7801 gggagaagtt ctccgcccgc gaggtttcgc cgacaatcag gatggtcaaa tttttacgtt
     7861 tttcgttctg cattaacggg ttgcggtggg cgtcttcacc aattcgcacc agcggtagat
     7921 ttgccagtcg ctgatgggag taccatgacc agctggcaac aatgctgtta gaggggctta
     7981 aggatttcac cagctctttg ttattgcgga acaacgaggc gtagtcttta taaaacagtg
     8041 cggcgaccag caaaatcagt agtacagaaa ccagaatatt ggctccacgg aaaagaacac
     8101 tgcgcagacg cgaggtggca ggtttgattt ttatccagca ggcaatcagc gcagcaagca
     8161 cgccgctgaa tcccagcgtt aataacattt gcggtgtcat cagcgcataa ctttctgccg
     8221 gagtggtatc aataatattg gcaatcatcg agcggtcgat gacgatgccg taagtcatta
     8281 tgaaatattg tgcagccgcg ccaaccagaa taaacaggca ggccagtggt cgattaagcc
     8341 ataagaaaga gcttagtgtc aggacaatat taatcacgct gaaagcgacg accggcatcg
     8401 acaagaaaac cagtacgtta tgcagcgaat ccagcggcag cgcctgcaac acctgtttaa
     8461 aaaaggcaat attcaggcag atagagatat aaaaagcggc caacaatagc caggcgagta
     8521 aattcaaaga gggtcttttt agtaggcgct tcaacatcac ggtgtttcca tcgaacaaag
     8581 tgcgcatatg ctcgcaaagc aaaattaagc caaccttaag ttcttaaggt tggcttttat
     8641 gtttgctgga ttaaggggaa ttaatctttg cttattggtg catctaaggg atacgggttt
     8701 ttatgtagcc ggttgtaatt cagcgcatac atggcggtga tgaccatcag ggtgacaaat
     8761 gaccacataa cctctttcgc tccggacccc accacggccc agatgcagta gaggaaggca
     8821 atggtagtaa ctgccagata tgccgggcgt gctttaccaa agtgaccgtg tccgagcagc
     8881 agtaacgccg cacaggtgta aagatatggc accagtgtaa agatgaccga gacggaagaa
     8941 accagaccga actctttggt cgcgtttggt gaaatgctgc tgagctggaa gatggtcatc
     9001 aaaataccga cgataatcag ccccgccact ggcgtacccg ctttatttac acgggcaaaa
     9061 atcggtggga acagtccgtc atcggcagcg gctttcgccg tttgacccgc cagcaacgtc
     9121 cagccgccca gtgaacctaa gcaacccgca gctgcgcaga aggaaacaat ggccccgggg
     9181 gtgtcaccca acgccatccg tgcggcatca ccgaatggcg aagcagaaac gcgcagtgcg
     9241 gcattaggga tcatccccat aatcgcggtg gtagaaagta catagcaaac ggcggcaatc
     9301 aataccccac caatggtggc gatagggaca ttgcgtttcg ggtttttcac cacacctgcg
     9361 gcaacggagg cactttccac accgatgaac gaccacagcg taacgttaag ggtactttga
     9421 attgcaccga aggtacccag gccgctgacg ttccatgccg ccatataggt ttcaccacgg
     9481 aaccagaacc agccaaatac ggcaatcccg acgatgggga tcagcgccag cacggtggca
     9541 actgcctgca cacgggtgat cattttcgga ccaacaatgt tcagcaggac gaagatccac
     9601 agcaccacga cgcaggtgat ggttaatacc aatggatctt tcagaatcgg gaagaagtaa
     9661 cttaaatatc ctacgccaat gaccaccatg gcgatattgc cgatccagca ggccagccag
     9721 tagaggacgt tggtttgata accgagaaac gggccaaagc agcggcgggc gtaagcgtaa
     9781 gaaccaccag gacttgggtc gaggaacgac attttggcgt ataccatcga gagccccagt
     9841 gcaccgataa tcgtcaccaa ccatccataa atggcaatcc cgccagtgga ggccaggttt
     9901 gcaggtaaca gaaaaacacc tgaccccata atattccccg acaccatcag ggtgacgggg
     9961 attaagccca ctttgtgagc atcagcatcc gaagacataa ttaaactcct gcgaaggcga
    10021 gcttcgtgac aataaattac gtcatatcat acgcctgcat tcgatagttg aattattatc
    10081 gaccgggtta tcgctgatgc cgttattaat atagctcttc gttaaggtta aaaatcaggc
    10141 gacagtgcgt tctctgtgct gactaacata atgcagcggc gtcataccgt agaagtcttt
    10201 aaagacagaa ataaagtacg acgtactgtt gtagccgcag gactgtgata cctgagagat
    10261 atttttaccg tccatcatta attcatttac ggcataacgc atgcggcagg tggtgattat
    10321 ttggctataa ctggtgtttt cgcttttcag ctttttcttt aacagacttg ggctaagaca
    10381 taaacaactg gctaccatac taagattcca gtctttgtga atatcgcttt caataatttg
    10441 ataaacgctg tcacttacac aattacgtaa catatacatc attaatgaaa ggaatttttt
    10501 actgtcgaga aaacgggata gcaccgtgaa taataatgcg cgtgttcgtt ccttttctga
    10561 ctcagtttct gcgggcatca tgctgtgttg cgcggcaacg cgaaagacgt ctggcgtcag
    10621 gcatggcagg gtgagtatgg gcgtagcgct acgttgccat actggtattt gcgagagatc
    10681 tttattcaga aaccggaggt aatctttgat gatgtcgtga ctaatatgag caaccaaagt
    10741 gttgttaaga gaggagacgt cgataatatt attttcacaa tttaataacg ccatatggtt
    10801 agctttaagg ctaataggtt ctttcccatt cacccttatc cagacatctt tttcagtcaa
    10861 taaaacaata caaggttggt cgctgcaaat cctcatacat gtactcctga gtgcgaataa
    10921 aagaccagtt ctcgctaaag caaagcgata ccgagaaatg gttgtataat tgtatgacga
    10981 atataaacat gcataaaaat aacgagtttt actaatatta caaattttta tagtaatttc
    11041 tcatgtacat atttttttac agggtaaata acaggcaaaa aaaagcgcgg ggtggggccg
    11101 cgcaaaaagt atgacatttt tgtttgcagc aatttttatg aaaacgttgc catactcaaa
    11161 aggcctaagg ccaggcggcg taatgttatt taaacaatta cgccttcagc ggaatagtgg
    11221 ttacgctttc acacacatag cgtggtaaat accgtcaata atttcagtcc cttcagtttc
    11281 gtgttcaaat ccagggaaat ggtggtccca ggattgcagc gagcgtaaat aacttacttg
    11341 cggactgttt ttatcgccga agttttcacc agacagcagc atcgggattc ctggcggata
    11401 tgggataact gagtttgccg cgatgcgtcc tggcagattt tcaatggata ccagttcgac
    11461 attgttgtcg acaatcgcgt tgtacgcttc acgcggggtg atttccgcca ccggcaggcc
    11521 ggaataggct tcgttcaacc gtgcgccagg gttgttttct ttcagccagg caaacatggt
    11581 gtcacccaga tcgtgaatcc ccatgttcgc gtaagtgtca ggatattgtt caacaagttc
    11641 cggcatcacc tgcgccagcg gtgtgttggc gtcatagtgg cgtttgaagg agcaaagggt
    11701 gttaaccaga gtcccccatt tcccacgggt tacgcccata gagaacagga acataatttg
    11761 gaagtcagtg gtgcgggtag gcacaatgcc gtggcgacca agccaggcag tgaccagcgc
    11821 cgccggaaca ccggtttctt ccagttcacc atcttcaccc attcccggag caaggatgct
    11881 gactttaatc gggtcgagca tactccagtt atccggaata tctttgaagc cgtgccagct
    11941 ttcgcccgga tgcattaccc agtagtcctg aacggtggtc agcagtttgg ttggtgcgtc
    12001 agcaaagtca taggttttgc cggtttgtgg gtcggtgacg acttctttgt tccacggttt
    12061 gaagaaccag ctaccgtcag cggtgaactc tttatacagc cgcgccatcg cctgacggaa
    12121 atcaaccgct tcgtcgatca cttcctgcgt cagtgacagg ccgctgttgc cgtccatcat
    12181 cgacaccgcc acgtcgttgg atgcgcagat ggcatacagc ggggaggtgg tggcatgcat
    12241 catgtaggcc tggttgaagc gggagaagtt aatcgcccca cgaccttcac gtacatgaat
    12301 ataagaagcc tgtgaaagcg cattcagcag tttgtgggtg gagtgggtgg cgaaaacggt
    12361 aggaccgttg tgatcgccag gttcgccgcg catggcatag tgatcggcat agatcgggtt
    12421 gaaacgtgca tagccgtacc aggcttcgtc aaagtgcaga cgatcggagg ttttttccag
    12481 cagatcctgc gcttctttag cgttataaca cacgccgtca taggtgcagt tggtcaccac
    12541 gcagtaagac ggtttttgcc cggctttgtc tttagtcagc gggctttcac tgattttctt
    12601 ctgcaaggtt tcaggttgca tttcctgcgg atagattggc ccgataatgc cgtagcggtt
    12661 gcggcttggc accatgtaga ccggtttcgc gcctgtcagc atcaagcctt gttcgatgga
    12721 tttatggcag ttacggtcaa cgaccacgac atcgttatcg gtcatgcaag cctgcatgat
    12781 ggtgcggtta gagccggaag taccgacgac taccgaccag gagcgatcgg caccaaatac
    12841 gcgtgcggca tatttttcgc tttcgccaaa tgcgccagta tggtcaagca aagaaccgag
    12901 ggaagttcgt tcgatgccca tatcggtgcg gaacagattt tctccatagt agtcatggta
    12961 gaaacgtccg gcgggtgttt tggtaaaacc aacgccgccc tggtggcctg gcgctgccca
    13021 ggaatattca tggatgtcgc tatacttcat cagcgcgctg aacagtggcg gcaacagctg
    13081 ctggcggtag cgggtcatcg cggcaacggc gcgtccggcg ataaagtcgg tggtatcttc
    13141 cagaatccag gcgaattcat cgacaagctc cagcaggtcg cgatccattg cggcgagggc
    13201 tttttcccga tcgcccaaca ggaagaccgg cacgttttgt tggcgctcat gaagcttacc
    13261 gatcaattgt ctgacgtttt gatgttcgtc cggatgttcc atttgatagc tgaacatcag
    13321 gcagtcaatg gcttcgtttg aagagagaat ggcaaaacca tcatcaaagg aggtggattt
    13381 aatcacggta acattttgct ggcttaaagc atctgccaga cgctcaacgg cgttaccgac
    13441 ccaggtgtct tgatggagaa actcgctttc aacaattaat actttcatca ttgcttaccc
    13501 ggttatgaag gaatcttcgt tgcgggcaag tatcttccgg ccttttcggg ctgtaaagcg
    13561 cgtgaaaaac aaacattttt gaaatattta tttttataaa caatgcgtta tgtgattatt
    13621 ttaaattatt ttcactgctt gttatcgacg gctaaactat ttttttggct gatactgata
    13681 tcgtctttag ccgggaaacg tctggcggcg ctgttggcta agtttgcggt attgttgcgg
    13741 cgacatgccg acatatttgc cgaacgtgct gtaaaaacga ctacttgaac gaaagcctgc
    13801 cgtcagggca atatcgagaa tacttttatc ggtatcgctc agtaacgcgc gaacgtggtt
    13861 gatgcgcatc gcggtaatgt actgtttcat cgtcaattgc atgacccgct ggaatatccc
    13921 cattgcatag ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg tcagcgcctg
    13981 atcatagttt tcggcaataa agcccagcat ctggctaaca taaaattgcg catggcgcga
    14041 gacgctgttt ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc agccagagag
    14101 gctaaatcgc ttgagcatca ggccaatttc atcaatggcg agctggcgaa tttgctcgtt
    14161 cggactgttt aattcctgct gccagcggcg cacttcaaac gggctaagtt gctgtgtggc
    14221 cagtgatttg atcatcatgc cgtgagtgac gtggttaatc aggtctttat ccagcggcca
    14281 ggagagaaac agatgcatcg gcagattaaa aatcgccatg ctctgacagg ttccggtatc
    14341 tgttagttgg tgcggtgtac aggcccagaa cagcgtgata tgaccctgat tgatattcac
    14401 tttttcattg ttaatcaggt attccacatc gccatcgaaa ggcacattca cttcgacctg
    14461 accatgccag tggctggtgg gcatgatatg cggtgcgcga aactcaatct ccatccgctg
    14521 gtattccgaa tacagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc tgcacataaa
    14581 cgtatctgta ttcatggatg gctctctttc ctggaatatc agaattatgg caggagtgag
    14641 ggaggatgac tgcgagtggg agcacggttt tcaccctctt cccagagagg cgaggggagc
    14701 gtccgagcat catgaggccg aaaactctgc ttttcaggta acttattacc ataaactcag
    14761 atttactgct gcttcacgca ggatctgagt ttatgggaat gctcaacctg gaagccggag
    14821 gttttctgca gattcgcctg ccatgatgaa gttattcaag caagccagga gatctgcatg
    14881 atgactgcac ccaaaattac atttatcggc gctggttcga cgattttcgt taaaaatatt
    14941 cttggtgatg tgttccatcg agaggcgctg aaaacggcgc atattgccct gatggacatt
    15001 gatcccaccc gcctggaaga gtcgcatatt gtggtgcgta agctgatgga ttcagcaggg
    15061 gccagcggca aaatcacctg ccacacccaa cagaaagaag ccttacagga tgccgatttt
    15121 gtcgtggtgg catttcagat tggcggttat gaaccttgca cggtgactga tttcgaggtc
    15181 tgtaagcggc atggtctgga acaaaccatt gccgatacgt tggggccggg cggtattatg
    15241 cgcgcgctac gtaccattcc gcatctgtgg caaatttgcg aggacatgac ggaagtctgc
    15301 cccgatgcca cattgctcaa ctacgttaac ccaatggcga tgaatacctg ggcgatgtat
    15361 gcccgctatc cgcatatcaa acaggtaggg ctgtgccatt cggtgcaggg aacggcggaa
    15421 gagctggcgc gtgacctcaa tatcgatccg tccacgctgc gttaccgttg cgctggtatc
    15481 aaccatatgg cgttttacct ggaactggag cgtaaaaccg ccgacggcag ttacgtgaat
    15541 ctctacccgg aactgctggc ggcttatgaa gcagggcagg caccgaagcc acatattcat
    15601 ggtaatactc gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct gggctacttc
    15661 gtcacggaat cgtcagaaca ttttgccgaa tatacgccgt ggtttattaa gccaggtcgt
    15721 gaggatttga ttgagcgtta taaagtaccg ctggatgagt acccgaaacg ctgcgtcgag
    15781 cagctggcga actggcataa agagctggag gagtataaaa acgcctcccg gattgatatt
    15841 aaaccgtcac gggaatatgc cagcacaatc atgaacgcta tctggactgg cgagtcgagt
    15901 gtgatttacg gcaacgtccg taacgatggt ttgattgata acctgccaca aggatgttgc
    15961 gtggaagtag cctgtctggt tgatgctaat ggcattcagc cgaccaaagt cggtacgcta
    16021 ccttcgcatc tggccgccct gatgcaaacc aacatcaacg tacagacgct gctgaccgaa
    16081 gctattctta cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga cccgcatact
    16141 gccgccgtgc tgggcattga cgaaatatat gctcttgttg acgacctgat tgccgcccac
    16201 ggcgactggc tgccaggctg gttgcaccgt taaaacgcga ctaaacgcta ctgcgccggg
    16261 ggatttattc cggcgcacac ctctgacgat accaataaca gaaggcgggc gttggtaaca
    16321 gcgacccgat accctatgag catttcaatg actacaaaac tcagttatgg atttggagcg
    16381 ttcgggaagg attttgcgat cggcattgtg tatatgtacc tcatgtatta ctacaccgat
    16441 gtcgttgggc tgtctgtggg tttggtcggt actttgtttc tggtggcgag gatctgggat
    16501 gctattaacg atccgattat gg
//
LOCUS       DAJVOY010000085        16480 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVOY010000085 DAJVOY010000000
VERSION     DAJVOY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.085"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(124..1407)
                     /gene="gltA"
                     /locus_tag="OQL90_002396"
     CDS             complement(124..1407)
                     /gene="gltA"
                     /locus_tag="OQL90_002396"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1369147.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1613..1720)
                     /locus_tag="OQL90_002397"
     CDS             complement(1613..1720)
                     /locus_tag="OQL90_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369148.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2101..2505
                     /gene="sdhC"
                     /locus_tag="OQL90_002398"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2101..2505
                     /gene="sdhC"
                     /locus_tag="OQL90_002398"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1369149.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2499..2846
                     /gene="sdhD"
                     /locus_tag="OQL90_002399"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2499..2846
                     /gene="sdhD"
                     /locus_tag="OQL90_002399"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1369150.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2846..4612
                     /gene="sdhA"
                     /locus_tag="OQL90_002400"
     CDS             2846..4612
                     /gene="sdhA"
                     /locus_tag="OQL90_002400"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1369151.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4628..5344
                     /gene="sdhB"
                     /locus_tag="OQL90_002401"
     CDS             4628..5344
                     /gene="sdhB"
                     /locus_tag="OQL90_002401"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1369152.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5645..8446
                     /gene="sucA"
                     /locus_tag="OQL90_002402"
     CDS             5645..8446
                     /gene="sucA"
                     /locus_tag="OQL90_002402"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1369153.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8461..9678
                     /gene="odhB"
                     /locus_tag="OQL90_002403"
     CDS             8461..9678
                     /gene="odhB"
                     /locus_tag="OQL90_002403"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1369154.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9954..11120
                     /gene="sucC"
                     /locus_tag="OQL90_002404"
     CDS             9954..11120
                     /gene="sucC"
                     /locus_tag="OQL90_002404"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1369155.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11120..11989
                     /gene="sucD"
                     /locus_tag="OQL90_002405"
     CDS             11120..11989
                     /gene="sucD"
                     /locus_tag="OQL90_002405"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1369156.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12093..12815)
                     /gene="mngR"
                     /locus_tag="OQL90_002406"
     CDS             complement(12093..12815)
                     /gene="mngR"
                     /locus_tag="OQL90_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1369157.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12984..14900
                     /gene="mngA"
                     /locus_tag="OQL90_002407"
     CDS             12984..14900
                     /gene="mngA"
                     /locus_tag="OQL90_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1369158.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMVNGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14918..>16480
                     /locus_tag="OQL90_002408"
     CDS             14918..>16480
                     /locus_tag="OQL90_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1369159.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKNQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQ"
BASE COUNT         3954 a         4133 c         4489 g         3904 t
ORIGIN      
        1 ttttagaata tatgaatgtc cgatagtgga ttttattttt acaatttttt gtgtttaggc
       61 atataaaaat taacccgcca tttgaacggc gggttaaaat atttacaact tagcaatcaa
      121 ccattaacgc ttgatatcgc ttttaaagtc gcgtttttca tatcctgtat acagctgacg
      181 cggacgggca atcttcatac cgtcactgtg catttcgctc cagtgggcga tccagccaac
      241 ggtacgtgcc attgcgaaaa tgacggtgaa catggaagac ggaataccca tcgctttcag
      301 gatgatacca gagtagaaat cgacgttcgg gtacagtttc ttctcgataa agtacgggtc
      361 gttcagcgcg atgttttcca gctccatagc cacttccagc aggtcatctt tggtgcccag
      421 ctctttcaga acttcatggc aggtttcacg cattacggtg gcgcgcgggt cgtaattttt
      481 gtacacgcgg tgaccgaagc ccatcaggcg gaaagaatca tttttatctt tcgcacgacg
      541 aacaaattcc ggaatgtgtt taacggagct aatttcttcc agcattttca gcgccgcttc
      601 gttagcacca ccgtgcgcag gtccccacag tgaagcaata cctgctgcga tacaggcaaa
      661 cgggttcgca cccgaagagc cagcggtacg cacggtggag gtagaggcgt tctgttcatg
      721 gtcagcgtgc aggatcagaa tacggtccat agcacgttcc agaatcggat taacttcata
      781 cggttcgcac ggcgtggaga acatcatatt caggaagtta ccggcatagg agagatcgtt
      841 gcgcggataa acaaatggct gaccaatgga atacttgtaa cacatcgcgg ccatggtcgg
      901 cattttcgac agcaggcgga acgcggcaat ttcacgatga cgaggattgt taacatccag
      961 cgagtcgtga tagaacgccg ccagcgcgcc ggtaatacca cacatgactg ccattggatg
     1021 tgagtcgcga cggaaagcgt ggaacagacg ggtaatctgc tcgtggatca tggtatgacg
     1081 ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc ggtttttcac cattcagcag
     1141 gatgtaacaa acttccaggt agttagaatc ggtcgccagc tgatcgatcg ggaaaccgcg
     1201 gtgcagcaaa ataccttcat caccatcaat aaaagtaatt ttagattcgc aggatgcggt
     1261 tgaagtgaag cctgggtcaa aggtgaacac accttttgaa ccgagagtac ggatatcaat
     1321 aacatcttga cccagcgtgc ctttcagcac atccagttca acagctgtgt ccccgttgag
     1381 ggtgagtttt gcttttgtat cagccattta aggtctcctt agcgccttat tgcgtaagac
     1441 tgccggaact taaatttgcc ttcgcacatc aacctggctt tacccgtttg ttatttggct
     1501 cgccgctctg tgaaagaggg gaaaacctgg gtacagagct ctgggcgctt gcaggtaaag
     1561 gatccattga tgacgaataa atggcgaatc aagtacttag caatccgaat tattaaactt
     1621 gtctaccact aataactgtc ccgaatgaat tggtcaatac ttccacactg ttacataagt
     1681 taatcttagg tgaaataccg acttcataac ttttacgcat tatatgcttt tcctggtaat
     1741 gtttgtaaca actttgttga gtgattgtca aattagatga ttaaaaatta aataaatgtt
     1801 gttgtcgtga cctggatcac tgttcaggat aaaacccgac aaactatatg taggttaatt
     1861 gtaatgattt tgtgaacagc ctatactgcc gctaggtctc cggaacaccc tgcaatcccg
     1921 agccacccag cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt gcatgacgcg
     1981 cagttataga aaggacgctg tctgacccgc aagcagaccg gaggaaggaa atcccgacgt
     2041 ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc cagggaataa taagaacagc
     2101 atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa tctggaccta
     2161 cagaccatcc ggttccccat cacggcgata gcgtccattc tccatcgcgt ttccggtgtg
     2221 atcacctttg ttgcagtggg catcctgctg tggcttctgg gtaccagcct ctcttcccct
     2281 gaaggtttcg agcaagcttc cgcgattatg ggcagcttct tcgtcaaatt tatcatgtgg
     2341 ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta ttcgccacat gatgatggat
     2401 tttggctatc tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat ctcctttgtt
     2461 attactgtcg tgctttcact tctcgcagga gtcctcgtat ggtaagcaac gcctccgcat
     2521 taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc taccgctatc gtcctgacgc
     2581 tctacatcat ttatatggtc ggttttttcg ccaccagtgg cgaactgaca tatgaagtct
     2641 ggatcggttt cttcgcctct gcgttcacca aagtgttcac cctgctggcg ctgttttcta
     2701 tcttgattca tgcctggatc ggcatgtggc aggtgttgac cgactacgtt aaaccgctgg
     2761 cattgcgcct gatgctgcaa ctggtgattg tcgttgcact ggtggtttac gtgatttatg
     2821 gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc agagaatttg atgcagttgt
     2881 gattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa atttcccaga gcggccagac
     2941 ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat accgtttctg cgcaaggcgg
     3001 cattaccgtt gcgctgggta atacccatga agataactgg gaatggcata tgtacgacac
     3061 cgtaaaaggg tcggactata tcggtgacca ggacgcgatt gaatatatgt gtaaaaccgg
     3121 gccggaagcg attctggaac tcgaacacat gggcctgccg ttctcgcgtc ttgatgatgg
     3181 tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg agcaggcggc
     3241 acgcactgcg gcggcggctg accgtaccgg tcacgcactg ttgcacacgc tttatcagca
     3301 gaacctgaaa aaccacacca ccattttctc cgagtggtat gcgctggatc tggtgaagaa
     3361 ccaggatggc gcggtggtgg gttgtactgc actgtgcatc gaaactggtg aagtggttta
     3421 tttcaaagcc cgcgctaccg tgctggcgac tggcggggca gggcgtattt atcagtccac
     3481 cactaacgcc cacattaaca ctggcgacgg cgtcggcatg gctatccgcg caggcgtacc
     3541 ggtgcaggat atggaaatgt ggcagttcca cccgaccggt attgccggtg cgggcgtact
     3601 ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg aacaaacatg gcgaacgctt
     3661 tatggagcgt tatgcgccga acgccaaaga cctggcgggc cgtgatgtgg tggcgcgttc
     3721 catcatgatc gaaatccgtg aaggtcgcgg ctgtgatggt ccgtgggggc cacacgcgaa
     3781 actgaaactc gaccacctgg gtaaagaagt tctcgaatcc cgtctgccgg gcatcctgga
     3841 actctcccgt accttcgctc acgtcgatcc ggtgaaagag ccgatcccgg ttatcccaac
     3901 ctgtcattac atgatgggcg gtattccgac caaagtgacc ggtcaggcgc tgactgtgaa
     3961 tgagaaaggc gaagatgtgg ttgttccggg actgtttgcc gttggtgaaa tcgcttgtgt
     4021 atcggtacac ggcgctaacc gtctgggcgg caactcgctg ctggacctgg tggtctttgg
     4081 tcgtgcggca ggtctgcatc tgcaagagtc tatcgccgag cagggcgcat tgcgcgatgc
     4141 cagcgagtct gatgttgaag catctctgga tcgtctgaac cgctggaaca ataatcgtaa
     4201 cggtgaagat ccggtggcga ttcgtaaagc gctgcaagaa tgtatgcagc ataacttctc
     4261 ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag cagttgaaag ttatccgcga
     4321 gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag ttcaacaccc agcgcgttga
     4381 gtgcctggaa ctggataacc tgatggaaac ggcgtatgca acggctgttt ctgccaactt
     4441 ccgtaccgaa agccgtggcg cgcatagccg cttcgacttc ccggatcgtg atgatgaaaa
     4501 ctggctgtgc cactccctgt atctgccaga gtcggaatcc atgacgcgcc gaagcgtcaa
     4561 catggaaccg aaactgcgcc cggcattccc gccgaagatt cgtacttact aatgcggaga
     4621 caggaaaatg agactcgagt tttcaattta tcgctataac ccggatgttg atgatgctcc
     4681 gcgtatgcag gattacaccc tggaagcgga agaaggtcgc gacatgatgc tgctggatgc
     4741 gcttatccag ctgaaagaga aagatcccag cctgtcgttc cgccgctcct gtcgtgaagg
     4801 tgtgtgcggt tccgacggtc tgaacatgaa cggcaagaat ggtctggcct gtattacccc
     4861 gatttcggca ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc caggtttacc
     4921 ggtgatccgc gatttggtgg tagacatggg acaattctat gcgcaatatg agaaaattaa
     4981 gccttacctg ttgaataatg gacaaaatcc gccagctcgc gagcatttac agatgccaga
     5041 gcagcgcgaa aaactcgacg ggctgtatga atgtattctc tgcgcatgtt gttcaacctc
     5101 ttgtccgtct ttctggtgga atcccgataa gtttatcggc ccggcaggct tgttagcggc
     5161 atatcgtttc ctgatcgata gccgtgatac cgagactgac agccgcctcg acggtttgag
     5221 cgatgcattc agcgtattcc gctgtcacag catcatgaac tgcgtcagtg tatgtccgaa
     5281 ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg atgttgttgc aacgtaatgc
     5341 gtaaaccgta ggcctgataa gacgcgcaag cgtcgcatca ggcaaccagt gccggatgcg
     5401 gcgtgaacgc cttatccggc ctacaagtca ttacccgtag gcctgataag cgcagcgcat
     5461 caggcgtaac aaagaaatgc aggaaatctt taaaaactgc ccctgacact aagacagttt
     5521 ttaaaggttc cttcgcgagc cactacgtag acaagagctc gcaagtgaac cccggcacgc
     5581 acatcactgt gcgtggtagt atccacggcg aagtaagcat aaaaaagatg cttaagggat
     5641 cacgatgcag aacagcgctt tgaaagcctg gttggactct tcttacctct ctggcgcaaa
     5701 ccagagctgg atagaacagc tctatgaaga cttcttaacc gatcctgact cggttgacgc
     5761 taactggcgt tcgacgttcc agcagttacc tggtacggga gtcaaaccgg atcaattcca
     5821 ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt actcttcaac
     5881 gatctccgac cctgacacca atgtgaagca ggttaaagtc ctgcagctca ttaacgcata
     5941 ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg ggactgtggc agcaagataa
     6001 agtggccgat ctggatccgt ctttccacga tctgaccgaa gcagacttcc aggaaacctt
     6061 caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa ctcggcgagc tgctggaagc
     6121 cctcaagcaa acctactgcg gcccgattgg tgccgagtat atgcacatca ccagcaccga
     6181 agaaaaacgc tggatccaac agcgtatcga gtctggtcgc gcgactttca atagcgaaga
     6241 gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc cttgaacgtt acctcggcgc
     6301 aaaattccct ggcgcaaaac gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct
     6361 taaagagatg atccgccacg ctggcaacag cggcacccgc gaagtggttc tcgggatggc
     6421 gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt
     6481 cgacgagttc gccggtaaac ataaagaaca cctcggcacg ggtgacgtga aataccacat
     6541 gggcttctcg tctgacttcc agaccgatgg cggcctggtg cacctggcgc tggcgtttaa
     6601 cccgtctcac cttgagattg taagcccggt agttatcggt tctgttcgtg cccgtctgga
     6661 cagacttgat gagccgagca gcaacaaagt gctgccaatc accatccacg gtgacgccgc
     6721 agtgaccggg cagggcgtgg ttcaggaaac cctgaacatg tcgaaagcgc gtggttatga
     6781 agttggcggt acggtacgta tcgttatcaa caaccaggtt ggtttcacca cctctaatcc
     6841 gctggatgcc cgttctacgc cgtactgtac tgatatcggt aagatggttc aggccccgat
     6901 tttccacgtt aacgcggacg atccggaagc cgttgccttt gtgacccgtc tggcgctcga
     6961 tttccgtaac acctttaaac gtgatgtctt catcgacctg gtgtgctacc gccgtcacgg
     7021 ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa
     7081 acatccgaca ccgcgcaaaa tctacgctga caagctggag caggaaaaag tggcgacgct
     7141 ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt
     7201 agtggcagag tggcgtccga tgaacatgca ctctttcacc tggtcgccgt acctcaacca
     7261 cgaatgggac gaagagtacc cgaacaaagt tgagatgaag cgcctgcagg agctggcgaa
     7321 acgcatcagc acggtgccgg aagcagttga aatgcagtct cgcgttgcca agatttatgg
     7381 cgatcgccag gcgatggctg ccggtgagaa actgttcgac tggggcggtg cggaaaacct
     7441 cgcttacgcc acgctggttg acgaaggcat tccggttcgc ctgtcgggtg aagactccgg
     7501 tcgcggtacc ttcttccacc gccacgcggt gatccacaac cagtctaacg gttccactta
     7561 cacgccgctg caacacatcc ataacgggca gggcgcgttc cgcgtctggg actccgtact
     7621 gtctgaagaa gcagtgctgg cgtttgaata cggttatgcc accgcagaac cacgcactct
     7681 gaccatctgg gaagcgcagt tcggtgactt cgctaacggt gctcaggtgg ttatcgacca
     7741 gttcatctcc tctggcgaac agaaatgggg ccggatgtgt ggtctggtga tgttgctgcc
     7801 gcacggttat gaagggcagg ggccggagca ctcctccgcg cgtctggaac gttatctgca
     7861 actttgtgct gagcaaaaca tgcaggtgtg cgtaccgtct accccggcac aggtttacca
     7921 catgctgcgt cgtcaggcgc tgcgcgggat gcgtcgtccg ctggtcgtga tgtcgccgaa
     7981 atccctgctg cgtcatccgc tggcggtttc cagcctcgaa gaactggcga acggcacctt
     8041 cctgccagcc atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat
     8101 gtgttctggt aaggtttatt acgacctgct ggaacagcgt cgtaagaaca atcaacacga
     8161 tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg cataaagcga tgcaggaagt
     8221 gttgcagcag tttgctcacg tcaaggattt tgtctggtgc caggaagagc cgctcaacca
     8281 gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg attccgtttg gggcttctct
     8341 gcgttatgca ggccgcccgg cctccgcctc tccggcggta gggtatatgt ccgttcacca
     8401 gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc gaataaataa aggatacaca
     8461 atgagtagcg tagatattct ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc
     8521 gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc
     8581 gaaactgaca aagtggtact ggaagtaccg gcatcagcag acggcattct ggatgcggtt
     8641 ctggaagatg aaggtacaac cgtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc
     8701 aacagcgccg gtaaagaaac cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa
     8761 cgccagcagg cgtctctgga agagcaaaac aacgatgcgt taagcccggc gatccgtcgc
     8821 ttgctggctg aacataacct cgacgccagc gctattaaag gcaccggcgt gggtggtcgt
     8881 ctgacccgtg aagatgtgga aaaacatctg gcgaaatccc cggcgaaaga gtctgcaccg
     8941 gcagcggctg ctccggcggc gcaaccggct ctggctgcac gtagtgaaaa acgtgtcccg
     9001 atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc
     9061 atgctgacca cgttcaacga agtcaacatg aagccgatta tggatctgcg taagcagtac
     9121 ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa
     9181 gcggtggttg aagccctgaa acgttacccg gaagtgaatg cttctatcga cggcgatgac
     9241 gtggtttacc acaactattt cgacgtcagc atggcggttt ctacgccgcg cggcctagtg
     9301 acgccggttc tgcgtgatgt cgataccctc ggcatggcag acatcgagaa gaaaatcaaa
     9361 gagctggcag tcaaaggccg tgatggcaag ctgaccgttg aagatctgac cggtggtaac
     9421 ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt ctacgccgat catcaacccg
     9481 ccgcagagcg caattctggg tatgcacgct atcaaagatc gtccgatggc ggtgaatggt
     9541 caggttgaga tcctgccgat gatgtacctg gcgctgtcct acgatcaccg tctgatcgat
     9601 ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt tgctggaaga tccgacgcgt
     9661 ctgctgctgg acgtgtagta gtttaagttt cacctgcact gtagacagga taaggcatta
     9721 tcgccttctc cggcaattga agcctgatgc gacgcttaac gcgtcttatc aggcctacgg
     9781 gaagcaccaa tgtaggtcgg ataaggcgct tgcgccgcat ccgacaagcg atgcctgatg
     9841 cgacacttac gcgtcttatc aggcctacag gtgaccgaca atgcccggag gcgatacgaa
     9901 atattcggtc tacggtttaa aagataacga ttgctgaagg atggacagaa cacatgaact
     9961 tacatgaata tcaggcaaaa caactttttg cccgctatgg cttaccagca ccggtgggtt
    10021 atgcctgtac tactccgcgc gaagcagaag aagccgcttc aaaaatcggt gccggtccgt
    10081 gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg
    10141 taaacagcaa agaagacatc cgtgcttttg cagaaaattg gctcggcaag cgtctggtaa
    10201 cgtatcaaac agatgccaat ggccaaccgg ttaaccagat tctggttgaa gcagcgaccg
    10261 atatcgctaa agagctgtat ctcggtgccg ttgtagaccg tagttcccgt cgtgtggtct
    10321 ttatggcctc cactgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcagc
    10381 tgatccataa agttgcgctt gatccgctga caggcccgat gccgtatcag ggacgcgagc
    10441 tggcgttcaa actgggtctg gaaggtaaac tggttcagca gttcaccaaa atcttcatgg
    10501 gtctggcgac cattttcctg gagcgcgacc tggcgttgat cgaaatcaac ccgctggtca
    10561 tcaccaaaca gggtgatctg atttgcctcg acggcaaact gggcgctgat ggcaacgcac
    10621 tgttccgcca gcttgatctg cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag
    10681 cacaggctgc acagtgggaa ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg
    10741 ttaacggcgc aggtctggcg atgggtacga tggacatcgt taaactgcac ggcggcgaac
    10801 cggctaactt ccttgacgtt ggcggcggtg caaccaaaga acgtgtaacc gaagcgttca
    10861 aaatcatcct ctctgacgac aaagtgaaag tcgttctggt taacatcttc ggcggtatcg
    10921 ttcgttgcga cctgatcgct gacggtatca tcggcgcggt agcagaagtg ggtgttaacg
    10981 taccggtcgt ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag aaactggctg
    11041 acagcggcct gaatattatt gcagcaaaag gtctgacgga tgcagctcag caggttgttg
    11101 ccgcagtgga ggggaaataa tgtccatttt aattgataaa aacaccaagg ttatctgcca
    11161 gggctttact ggtagccagg ggactttcca ctcagaacag gccattgcat acggcactaa
    11221 aatggttggc ggcgtaaccc caggtaaagg cggtactacc cacctcggcc tgccggtgtt
    11281 caacaccgtg cgtgaagctg ttgctgctac tggcgctacc gcttctgtta tctacgtacc
    11341 agcaccgttc tgcaaagact ccattctgga agctattgat gcaggcatca aactgattat
    11401 caccatcact gaaggcatcc cgacgctgga tatgctgact gtgaaagtga aactggacga
    11461 agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt atcactccgg gtgaatgcaa
    11521 aatcggtatc cagccgggtc acattcacaa accgggtaaa gtgggtatcg tttcccgttc
    11581 cggtacactg acctatgaag cggttaaaca gaccacggat tacggtttcg gtcagtcgac
    11641 ctgtgttggt atcggcggtg acccgatccc aggctctaac tttatcgaca tcctcgaaat
    11701 gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc ggtgagatcg gcggtagcgc
    11761 tgaagaagaa gcggcagcgt acatcaaaga gcacgttacc aagccagttg taggttacat
    11821 cgctggtgtg actgcgccga aaggcaaacg tatgggccac gcgggtgcga tcattgccgg
    11881 tgggaaaggg actgcggatg agaaattcgc tgctctggaa gccgcaggcg tgaaaaccgt
    11941 tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat
    12001 aagaaatagc cctcgccgct tccctctaca ggaatggcga agggctgtcg gtttcgacat
    12061 ggttggccat cgtatgatgg ccttttttgt gcttatcgcg atgattttcg ctgcgctatc
    12121 agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc tatattcaaa cacccgacca
    12181 tcaactaaat atccacgcga tactttttca agaatcggct ttgtctggct gatattaagc
    12241 agacggctca tctcttcggt tggcatcaga ggaatgattt cctgttcgct acgatcgata
    12301 accatcttct tcacttcttc aataaagtga tatttcgaat tttccatgac ctgccaggtg
    12361 agatccggga acaacgcaag cggcatccag gtttcttcca gcgccattgg cttttgcttg
    12421 cgatagcgca cgcgcttcac atgccacaca cgatcctgcg cggtgatttg tagctgttgc
    12481 tgaagaaaat cgtcagccgg aatcacttca aatatcagga cttcactgtg tgtatcgacg
    12541 tgacggtccg acagtttttc atcaaaactg gttaactgaa aaatatcgta attgacccgc
    12601 tcttctttga cgtaagtccc gctgccctga atgctttcga ggatctgctg ctcgactagc
    12661 tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa actctgtttg tagcgctgat
    12721 tcagtgggta acgcatcgcc aggtttaagc tcgccacgcg caatttgttc acgaatgcga
    12781 tcggcaatct gccggtataa gggcttgtgt cccattttta gtatctcatt aatacgaatt
    12841 tagccattat gcccgataaa ttcatcctgt aaataatgca aatacaatac aaataatttc
    12901 aatcaagtga aattgatcat ataatggtat tgttttatcg ggcacactgg cgcgactata
    12961 aaaacgatca agtgaggatc atgatgaatc tgacgactct gacccaccgc gatgcgttgc
    13021 gtctgaatgc gcgctttacc agccgtgaag aggccatcca cgcgttgact caacgtcttg
    13081 ctgctctggg gaaaatttcc agtactgagc aatttctgga agaagtgtat cgccgtgaaa
    13141 gccttggccc gaccgcctta ggtgaagggt tggctgtgcc gcatggcaaa actgctgcgg
    13201 taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc gcttcagtgg gaaggcgttg
    13261 atggcccgga agcagttgat ttagtggtgc tgctggcgat tccccccaat gaagcgggta
    13321 ctatgcatat gcaactgctg acagcgctga ccacgcgcct tgcggatgat gagattcggg
    13381 cgcgtataca gtcggcgacg acgcctgatg agttgctctc ggcgctggat gacaagggag
    13441 gcacgcaacc ttctgcctct ttttccaacg cgccaactat cgtctgcgta ccggcctgtc
    13501 cggcgggtat tgctcacacc tatatggctg cggaatatct ggaaaaagcc ggacgcaaac
    13561 tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg cattgaaggg cgtttaacgg
    13621 cggatcaact caatagtgca accgcctgta tttttgcggc tgaagtcgcc atcaaggaga
    13681 gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt tgccgagccg attcgccatg
    13741 cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag cgatgagacg cgtaccgtac
    13801 agcaagatac gcaaccggtg aaaagtgtca aaacggagct gaaacaggca ctgttgagcg
    13861 gaatctcttt tgccgtaccg ttgattgtcg cggggggcac ggtgctggcg gtcgcggtat
    13921 tactgtcgca aatcttcggg ctacaagatc tgtttaatga agaaaactcc tggctgtgga
    13981 tgtaccgcaa gctgggcggc gggctgctcg gaattttgat ggtaccggtg ctcgcggcct
    14041 atactgccta ttctctggca gataaaccgg cgttagcgcc aggctttgcg gctggacttg
    14101 ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt tggcggattg atagccggtt
    14161 acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag taaattcaat ggattcctga
    14221 ctttttatct ctacccggtg ctcggtacgt tgggagcggg cagtctgatg ctgtttgtgg
    14281 tgggagaacc tgtcgcatgg atcaataact cgcttaccgc ctggctgaac ggtctgtcag
    14341 gaagtaacgc gctgttgctg ggtgccattt tcggttttat gtgttccttt gatcttggag
    14401 ggccagtgaa caaagccgct tatgcattct gcctgggcgc aatggtgaac ggcgtttacg
    14461 gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc atttaccgta accgcttcca
    14521 cgatgctcgc accgcgcctg tttaaagagt ttgaaattga gaccgggaaa tccacctggc
    14581 tgttagggct ggcaggtatt accgaagggg cgatcccgat ggcgattgaa gatccgctgc
    14641 gggttattgg ttcgtttgtg ctgggctcta tggtaacggg cgctattgtc ggtgcgatga
    14701 atatcggcct ttcgacaccc ggtgccggca ttttctcgct ctttttactt catgataatg
    14761 gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc ggcattggtg ggggctgcaa
    14821 tctcgactgc aattctcctg atctggcggc gtcacgcggt taagcatggc aactatctga
    14881 ctgatggcgt aatgccataa acaaaaagga aacgacgatg aaagcggtat ctcgtgttca
    14941 catcaccccg catatgcact gggatcgaga gtggtatttc accaccgaag agtcacgtat
    15001 tctgctggtc aataatatgg aagagatcct gtgccgactg gaacaggaca acgaatacaa
    15061 atattacgta ctcgacgggc aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga
    15121 aaacaaagac cgtgtgaaga accaggtaga agccggcaag ttgattatcg gcccctggta
    15181 tacccagacc gataccacga ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg
    15241 aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata ggttatttac cagattcctt
    15301 tggcatgtcc gggcaactgc cgcatatcta caatggattt ggcattaccc gcaccatgtt
    15361 ctggcgcgga tgttcggagc gccacggtac tgataaaacc gagtttttgt ggcaaagcag
    15421 tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc tacgccatag gtaagtactt
    15481 acctaccgac gaaaacggat tacgtaaacg cctcgacagt tattttgacg tgctggaaaa
    15541 agcgtctgta accaaagaga ttttgctgcc gaatggtcat gaccagatgc cattgcagca
    15601 aaatatcttc gaagtgatgg ataagctacg tgagatctac cctcaacgta agtttgtgat
    15661 gagccgcttt gaagaggtat ttgagaagat cgaagcgcag cgtgaaagtc tggccaccct
    15721 gaaaggtgaa tttatcgatg gcaaatatat gcgcgtacat cgcaccatcg gttccacgcg
    15781 catggatatc aaaattgccc acgcacgtat tgaaaataag attgttaatc tgctggaacc
    15841 gctggcaacg ctggcctgga cgttgggttt tgaataccac cacggcttgc tggagaaaat
    15901 gtggaaagag atcttaaaaa atcatgccca cgacagtatc ggttgttgtt gcagtgacaa
    15961 agttcatcgc gaaattgtcg cccgcttcga actggctgaa gacatggcgg ataatctgct
    16021 gcgtttctac atgcgcaaaa ttgccgataa catgccacag agtgatgccg acaaactcgt
    16081 gctgtttaac ctgatgccct ggccgcggga agaagttatt aacaccactg tgcggctgcg
    16141 cgccagccag tttaatctac gagacgatcg cggtcagcct gtaccgtatt ttattcgcca
    16201 tgcccgtgag atcgatccag gcctaatcga tcggcaaata gttcattacg gtaattacga
    16261 tccctttatg gagtttgata tacagatcaa ccagattgtc ccttctatgg gctatcgcac
    16321 gctttatatc gaagcgaatc agcctggcaa cgtgattgcg gcaaaaagtg acgctgaagg
    16381 gatactggaa aatgctttct ggcaaattgc gctcaatgag gatggttctc tgcaactggt
    16441 agataaagac agcggtgtgc gctatgaccg agtattgcaa
//
LOCUS       DAJVOY010000086        16316 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVOY010000086 DAJVOY010000000
VERSION     DAJVOY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.086"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..1129)
                     /locus_tag="OQL90_002409"
     CDS             complement(<1..1129)
                     /locus_tag="OQL90_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCS1369160.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVN"
     gene            complement(1126..1344)
                     /gene="ybdZ"
                     /locus_tag="OQL90_002410"
     CDS             complement(1126..1344)
                     /gene="ybdZ"
                     /locus_tag="OQL90_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS1369161.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(1347..2549)
                     /gene="fes"
                     /locus_tag="OQL90_002411"
     CDS             complement(1347..2549)
                     /gene="fes"
                     /locus_tag="OQL90_002411"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS1369162.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWREPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            2792..5032
                     /gene="fepA"
                     /locus_tag="OQL90_002412"
     CDS             2792..5032
                     /gene="fepA"
                     /locus_tag="OQL90_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS1369163.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            5207..5827
                     /gene="entD"
                     /locus_tag="OQL90_002413"
     CDS             5207..5827
                     /gene="entD"
                     /locus_tag="OQL90_002413"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS1369164.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(5949..6101)
                     /gene="hokE"
                     /locus_tag="OQL90_002414"
     CDS             complement(5949..6101)
                     /gene="hokE"
                     /locus_tag="OQL90_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1369165.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            6555..7673
                     /locus_tag="OQL90_002415"
     CDS             6555..7673
                     /locus_tag="OQL90_002415"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1369166.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            7739..7987
                     /locus_tag="OQL90_002416"
     CDS             7739..7987
                     /locus_tag="OQL90_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCS1369167.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPTPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            8052..8420
                     /locus_tag="OQL90_002417"
     CDS             8052..8420
                     /locus_tag="OQL90_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1369168.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            8514..9167
                     /gene="nfsB"
                     /locus_tag="OQL90_002418"
     CDS             8514..9167
                     /gene="nfsB"
                     /locus_tag="OQL90_002418"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS1369169.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            9275..10522
                     /gene="ybdG"
                     /locus_tag="OQL90_002419"
     CDS             9275..10522
                     /gene="ybdG"
                     /locus_tag="OQL90_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1369170.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(10590..11966)
                     /gene="pheP"
                     /locus_tag="OQL90_002420"
     CDS             complement(10590..11966)
                     /gene="pheP"
                     /locus_tag="OQL90_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1369171.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(12068..15195)
                     /gene="cusA"
                     /locus_tag="OQL90_002421"
                     /pseudo
     CDS             complement(12068..15195)
                     /gene="cusA"
                     /locus_tag="OQL90_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(15207..>16316)
                     /gene="cusB"
                     /locus_tag="OQL90_002422"
     CDS             complement(15207..>16316)
                     /gene="cusB"
                     /locus_tag="OQL90_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCS1369172.1"
                     /translation="ERKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGV
                     RIDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTV
                     GDKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRL
                     IATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAW
                     LVKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAW
                     LQLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGLA
                     EGEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         3985 a         4452 c         4127 g         3752 t
ORIGIN      
        1 cattaaccgg accggttgcc aaggtatggg tttgcgcctg aacaccagga atatccagtt
       61 ggtaatcaaa taccttgata ttgagtaccg gaccaaacag cggttcatca cctgccgctc
      121 gcccgctgtc acggacaatt tgttcggcat cgtaacgttg atgacgacgc atttttttca
      181 gttgtgctgc cagtcgggtt gccagttccg gcagcgtttc ttgcgccgca atgtgaatac
      241 ccaacggcaa aacgttgagc acgggtccgg tagccgtcag cgccgccgag cccagtcgac
      301 gcataaagat aaatccagcg gcgtagtcca tgcgattgca caatcgcccc agccacaaag
      361 ccgtcagcgc aagggctaaa tcggtacgct gcacacctga aagttgcgta gccaactggc
      421 ggaattcccc gtcggtaaat tccattttca gacgtaaaat atctgccgaa gcgctgcgcc
      481 ccggtaaagg tgccggagaa agtgacgcgg gcggcggcag ttgacgacgc tgttctgccc
      541 agaatgccgc atcacgctgc caggcttcgc tttcgcggta ttgctggtac tcttccacta
      601 catcagcgaa aggcgtaaac ggcgaagcag gcgttggttc accacgcagc catgtgcagt
      661 aaatattggc gatctggcgg gtaatggccg ggaaactgaa gccatcgacc agcaaatggt
      721 gataacgctg ataccagtac cagcggttat ccgccacctg tatcagctga tgaaagacca
      781 gtggtttacc gctatcgacg cgcagatctt gttgcaaatc cgcctgcatt aatgcctgcg
      841 cagtaccgtg cggatcaatg ttggttcgca ggtcgataat ttctggcagt tcgaacgtca
      901 gcgcatcatc gacccactgc cagacttcgc cgttatcttc cgtaaaacgc atccgcagcg
      961 tatctgcttg cgctagtccg tcaaccaccg cgcgggccag taatggcgca tcaacctctc
     1021 cggttaactc aacgtaatgc gctacgctcc aggcggaggg taaatccgac agtttttctg
     1081 ccatccagat gccgggctgt gcggcgacca aaggtaaatg ctggctcatt gcgcctcctg
     1141 caactgggtg aaattcgccg gtgtcagagt acgccagtgg gcttcaagcc actgctggca
     1201 ggactcttgt gactgcggct gacacacaat gtcccagcct gccggtaagg cgcattgttg
     1261 cggccacaga ctgaattgcc cctgcgcatt gcgcaaaatg taaaacgctc cctgtggatc
     1321 atcgaaggga ttactgaatg ccatattcaa ctcctgtcat ggaaaagtgg ttgccagagg
     1381 tcgattagcc cctgcatcaa gccaccgcgc caacaaagcg catcatgtcc gccgtcaacc
     1441 tgacgccaga aaatggattc ttttatgggg tgtaattgcg catacagcgc ctgattggcc
     1501 cgcataatca tcggctcgcg aatacccgct tccagcacaa tgcgcagacc ttcggcgcta
     1561 acttcaccag ctttaagctt ttcaagtaac acgccctctt gctgcccgcc ccgatgcggc
     1621 caccagtacg atcctgactg gcttaataca caaccaaaac gttcaggcca gtgcagtccg
     1681 gcatacagcg cggaaagtcc accaaaactc tgcccggcaa ccacggtgcg atcggcgcga
     1741 tcgctaaaag gggcaatagc tttcaccagg ggtaataact cttgctgtac tgcgagccag
     1801 aaatccgcat tacacggcag ttcgtgagcg cggtgcgtgg tgtcgatagc gtcgatcaac
     1861 acatacacgg cgggaggaag ttgctgacga tgggtcagcg aagtcagcac tggccagacg
     1921 ggcatacttt gcgcccaaaa ttcgccatcg agcaaaactg ccagcgggcg ttcttctgct
     1981 gttgcatcgc cggtggtaaa aatccataca cgacgtgaat ttttcaaccg ttcacttttc
     2041 cagataattt ctttggctgg tatttctggc gcttgcggac aatcccatcc cggttgcaga
     2101 ggcgcttgcg gcatttcgag tgcagaaaca gcgtgccctc gcccgccttt ccagctttgt
     2161 ggattcagcg gatcggctat tgcctggggt aatagttttc gccagccttc acgtaattcg
     2221 aggcgatcgg gggatggtgc agaaaaaatg tcatcgcgtt cggtgggaat aaagcagtag
     2281 ctgccgcgcc agttggcatt gagttgtgtc gtccactgcc agacatcagt acctgcaatg
     2341 cgctgcatcg actggggctg gctgttctga tgatgatcgg tcacaccagt gatgtagacc
     2401 catacgcgct ttatcggcga gtattcttca gaaccttggg gctcacgcca ccagaaagtg
     2461 acctcaaaca tttcgtcatt cagacgctgc cattccgggc catgtttcga ctgccaccag
     2521 ctctcacttc ctacttttaa cgccgtcaca ccataacccc atgtttactg tgcaattttt
     2581 cattgattgc agaaatatat tgataatatt attgataact atttgcattt gcaatagcgt
     2641 aatggcgcgc cgtgggaagc gcggacatta attaaccaac tgcactgcgt gtctttcagg
     2701 atcaaaggtt ttcgcggtag cgggatgcgt cgtgttgagg acgaccatgc ccgacagttg
     2761 caattcgtgg caaaaatgca ggaataaaac aatgaacaag aagattcatt ccctggcctt
     2821 gttggtcaat ctggggattt atggggtagc gcaggcacaa gagccgaccg atactcctgt
     2881 ttcacatgac gataccattg tcgttaccgc cgccgagcag aacttgcagg cacctggcgt
     2941 ttcgaccatc accgcagatg aaatccgcaa aaacccggtt gcccgcgatg tgtcggagat
     3001 catccgtacc atgccaggcg ttaacctgac cggtaactcc accagtggtc agcgtgggaa
     3061 taaccgacag attgatattc gcggtatggg tccggaaaac acgctgattt taattgacgg
     3121 caaaccggtc agcagccgta actcggtgcg tcagggctgg cgtggcgagc gcgatacccg
     3181 tggtgatact tcctgggtgc cgcctgaaat gattgaacgt attgaagttc tgcgtggtcc
     3241 ggctgcagcg cgttatggca acggcgcggc gggtggcgta gttaacatca ttaccaaaaa
     3301 aggcagcggt gagtggcacg gttcctggga cgcttatttc aatgcgccag aacataaaga
     3361 ggaaggtgcc actaaacgca ccaacttcag tctgaccggc ccgctgggcg acgaattcag
     3421 cttccgcttg tatggcaacc tcgacaaaac ccaggctgac gcgtgggata tcaaccaggg
     3481 ccatcagtcc gcgcgtgccg gaacgtatgc cacgacgtta ccagccgggc gcgaaggggt
     3541 gatcaacaaa gatattaatg gcgtggtgcg ctgggacttc gcgcctctgc aatcactcga
     3601 actggaagcg ggttacagcc gccagggtaa cctgtatgcg ggcgataccc agaacaccaa
     3661 ctctgacgct tacactcgct cgaaatatgg cgatgaaacc aaccgcctgt atcgccagaa
     3721 ctactcgctg acctggaacg gtggctggga taacggagtg accaccagca actgggtgca
     3781 gtatgaacac acccgtaact cgcgtattcc ggaaggtctg gcgggcggta ccgaagggaa
     3841 atttaacgaa aaagcgacac aggatttcgt cgatatcgat cttgatgacg tgatgctgca
     3901 cagcgaagtt aacctgccga ttgatttcct cgttaaccaa acgctgacgc tgggtacgga
     3961 gtggaatcag caacggatga aggacttgag ttccaatacc caggcgctga ccggggcgaa
     4021 caccggcggc gctattgatg gtgtgagtgc caccgaccgt agcccgtatt caaaagcaga
     4081 aattttctcg ctatttgccg aaaacaacat ggagctgact gacagcacca tcgtaacgcc
     4141 ggggctgcgt ttcgatcatc acagtattgt cggcaataac tggagcccgg cgctgaacat
     4201 atcgcaaggt ttaggcgatg acttcacgct gaaaatgggc attgcccgcg cctataaagc
     4261 gccgagcctg taccagacta acccgaacta cattctctac agtaaaggtc agggctgcta
     4321 tgccagcgcg ggcggctgct atctgcaagg taacgatgac ctgaaagcag aaaccagcat
     4381 caacaaagag attggtctgg agttcaaacg cgacggctgg ctggcgggcg tcacctggtt
     4441 ccgtaacgat tatcgcaata agattgaagc gggctatgtg gctgtagggc aaaacgcagt
     4501 cggcaccgat ctctatcagt gggataacgt gccgaaagca gtggttgaag gtctggaagg
     4561 atcgttaaac gtaccagtta gcgaaacggt gatgtggacc aataacatca cttatatgct
     4621 gaagagtgaa aacaaaacca cgggcgaccg tttgtcgatc atcccggagt acacgttgaa
     4681 ctcaacgctg agctggcagg cgcgggaaga tttgtcgatg caaacgacct tcacctggta
     4741 cggtaagcag cagccgaaaa aatacaacta caaaggtcag ccagcggttg gcccggaaac
     4801 caaagaaatc agtccttaca gcattgtggg tctgagcgcg acctgggatg tgacgaagaa
     4861 tgtcagtctg accggcggcg tggacaacct gttcgacaaa cgtttgtggc gtgcgggtaa
     4921 tgcccagacc acgggcgatc tggcaggggc caactatatc gcgggtgccg gggcgtatac
     4981 ctataacgag ccgggacgta cgtggtacat gagcgtaaac actcacttct gatgctaacg
     5041 tcagattgtt gataaagtgc gcgtcgttca tgccggatgt ggcgtgaacg ccttatccgg
     5101 cctacaaaaa tcttgccaat tcaatatatt gcaggaccat gtaggcctga taagcgtagc
     5161 gcatcaggca gtttagcgtt tgtcaccagt ctctaatatg gtcgacatga aaactacgca
     5221 tacctccctc ccctttgccg gacatacgct gcattttgtt gagttcgatc cggcgaattt
     5281 ttgtgagcag gatttactct ggctgccgca ttacgcgcaa ctacaacacg ctggacggaa
     5341 gcgcaaagcc gaacatttag ccgggcggat cgctgcaatt tatgcgctgc gggaatatgg
     5401 ctataaatgt gtgcccgcaa tcggcgagct acgccaaccg gtgtggcctg cggaggtata
     5461 cggcagtatt agccactgcg gggctacggc attggccgtg gtttcccgtc aaccgattgg
     5521 cgttgatatc gaagaaattt tttctgcgca aacagcgacc gaattgacag acaacattat
     5581 tacaccagca gaacacgagc gactcgcaga ctgcggttta gccttttctc tggcgctgac
     5641 actggcattt tccgccaaag agagcgcatt taaggcaagt gagatccaaa ctgatgcagg
     5701 ttttctggac tatcagataa ttagctggaa taaacagcag gtcatcattc atcgtgagaa
     5761 tgagatgttt gctgtgcact ggcagataaa agaaaaaata gtcataactc tgtgccaaca
     5821 cgattaattg acaacatctg gtacgattcg cccgcagcca tcactgaccg cgggcgaaag
     5881 tgtaaagcag gtgccttacc atcctgacct gacaaccgga tatgcgggga ttgctccccg
     5941 cacaacggct acttcttcgg ttcgtaagcg agaacagcct taaactcaat attacgttcc
     6001 tttacggtga actcacacag cgaatccccg accagaagcg taaatcccag caccgttaaa
     6061 cacagcacta tgactgccgc aagggcatat ttcgtcagca tgtttatatt gcctccatgc
     6121 gaagaaggga ctaccatcca aattgttcag gtttgggtag gtagccccag ggttgataga
     6181 aatatcgtcc tggggcttca tctttccaga cctcagagta aacctgaaac cggaaagctt
     6241 caggcacccg cagttatctt acccttgctt agcaaaatag ctgtttattt atacagtaag
     6301 tcctgtttcg gacttctcac tccccatttg atcaactgaa gctgaacccg tttttttctg
     6361 catgcctcaa tcgtcaaaat tcttcgccac cagtcagcac tgccataact tgtcacattg
     6421 caaaagtagc caaattgctt ctgtcataat cagttcacca tcaccaaaaa aaagaaacat
     6481 tccagaactt ccgcatctcg cccagccgct cccacttcta tactgaatag aaaacgccaa
     6541 cataagagaa acctatgcca ttacccgatt ttcatgtttc tgaacctttt acactcggta
     6601 ttgaactgga aatgcaggtg gttaatccgc cgggctatga cttaagccag gactcttcaa
     6661 tactgattga cgcggttaaa aataagatca cggccggaga ggtaaagcac gatatcaccg
     6721 aaagtatgct ggagctggcg acggatgttt gccgtgatat caaccaggct gccgggcaat
     6781 tttcagcgat gcagaaagtc gtattgcagg cagccgcaga ccatcatctg gaaatttgcg
     6841 gcggtggcac acacccgttt cagaaatggc agcgacagga ggtatgcgat aacgagcgct
     6901 atcaacgaac gctggaaaac tttggttatc tcattcaaca ggtgaccgtt tttggtcagc
     6961 atgttcatgt tggctgcgcc agtggcgatg acgccattta tttgctgcac ggcctgtcac
     7021 ggtttgtgcc gcacttcatc gccctttccg ccgcgtcgcc atatatgcaa ggaacggata
     7081 cgcgttttgc ctcctcacga ccgaatattt tttccgcctt tcctgataat ggcccgatgc
     7141 cgtgggtcag taactggcaa caatttgaag ccctgtttcg ctgtctgagt tacaccacga
     7201 tgatcgacag cattaaagat ctgcactggg atattcgccc cagtcctcat tttggcacgg
     7261 tggaggttcg ggtgatggat accccgttaa cccttagcca cgcggtaaat atggcgggat
     7321 taattcaggc taccgcccac tggttactga cagaacgccc gttcaaacat caggagaaag
     7381 attacctgct gtataaattc aaccgtttcc aggcctgtcg ctatgggctg gaaggcgtca
     7441 ttaccgatcc gcacactggc gatcgtcgac cgctaacgga agacaccttg cgattgctgg
     7501 aaaaaatcgc cccttccgca cataaaattg gtgcatcgag cgcgattgag gccctgcatc
     7561 gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg cgatttcgtc gccgatggtg
     7621 gctcgctgat tgggctggtg aaaaagcatt gtgagatctg ggccggtgac taagtaggga
     7681 attgccttac agaagaatat ccctgacaat ggtgttttaa cttacattta acaatgttat
     7741 gaaacaccct ttagaaacct tgactaccgc agcaggcatt ttgctgatgg ctttcctctc
     7801 ttgcctgctg ctgcccaccc ccgcactggg gctgacgctg gcacaaaaac tggtgaccat
     7861 gttccatctg atggatctta gccagcttta cactttattg ttttgtctgt ggtttttagt
     7921 gctgggcgct attgagtatt ttgttctgcg ctttatctgg cgacgctggt tctcgctggc
     7981 ggattaaacg tcgattatcg ccgtacttcg cggcatactt tgcttattct ccttcgccta
     8041 aaggaacgtt tatggataag caatcactgc acgaaacggc gaaacgcctg gcccttgagt
     8101 taccctttgt cgagctttgc tggccttttg gcccggagtt cgatgttttt aaaattggcg
     8161 gcaaaatttt tatgctgtcg tcggagctgc gcggcgtccc ctttatcaat ctgaagtccg
     8221 atccacaaaa atccctgtta aatcagcaga tatacccgag cattaagcca gggtatcaca
     8281 tgaataaaaa gcactggatt tcggtgtatc ccggcgagga aatctccgaa gcgttacttc
     8341 gcgatctgat caacgattcg tggaatctgg tggttgatgg tctggctaaa cgcgatcaaa
     8401 aaagagtgcg tccaggctaa agcggaaatc tatagcgcat ttttctcgct taccatttct
     8461 cgttgaacct tgtaatctgc tggcacgcaa aattactttc acatggagtc tttatggata
     8521 tcatttctgt cgccttaaag cgtcattcca ctaaggcatt tgatgccagt aaaaaactta
     8581 ccccggaaca ggccgagcag atcaaaactc tcctgcaata cagcccatcc agcaccaact
     8641 cccagccgtg gcattttatt gttgccagca cggaagaagg taaagcgcgt gttgccaaat
     8701 ccgctgccgg taattatgtg ttcaacgaac gtaaaatact tgatgcctcg cacgtcgtgg
     8761 tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa gctggttgtt gaccaggaag
     8821 atgctgatgg ccgctttgcc acgccggaag cgaaagccgc gaacgataaa ggtcgcaagt
     8881 tcttcgctga tatgcaccgt aaagatctgc atgatgatgc agagtggatg gcaaaacagg
     8941 tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc tctgggtctg gacgcggtac
     9001 ccatcgaagg ttttgacgcc gccatcctcg atgcagaatt tggtctgaaa gagaaaggct
     9061 acaccagtct ggtggttgtt ccggtaggtc atcacagcgt tgaagatttt aacgctacgc
     9121 tgccgaaatc tcgtctgccg caaaacatca ccttaaccga agtgtaattc tctcttgccg
     9181 ggcatctgcc cggctatttc ccctcagatt ctcctgattt gcataaccct gtttcagccg
     9241 tcatcatagg ctgctgttgt ataaaggaga cgttatgcag gatttaatat cccaggttgg
     9301 ggatttagcg ggtattgaga tcgatcacac cacctcgatg gtgatgattt tcggtattat
     9361 ttttctgacc gccgtcgtgg tgcatattat tttgcattgg gtggtactgc ggaccttcga
     9421 aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc attacccaga ataaactctt
     9481 ccaccgttta gcttttaccc tgcaggggat tatcgtcaat attcaggcgg tattctggct
     9541 gcaaaaaggc accgaagcgg cagatattct gactacctgc gcgcagttgt ggatcatgat
     9601 gtatgcgctg ctttcagtct tctcgttgct ggatgttatt ttgaatctgg cgcagaaatt
     9661 cccggcggca tctcagttac cgctgaaagg gatatttcag gggattaaac tgatcggcgc
     9721 aatcctggtc ggcattttga tgatctcgct gctgattggt cagtcgccag cgattctgat
     9781 cagcggtctt ggtgcaatgg ctgccgtgct gatgttggta tttaaagatc cgattcttgg
     9841 tctggtggca ggtattcagc tttccgcgaa cgatatgctg aaactgggcg actggctgga
     9901 gatgccgaaa tacggcgcgg atggcgcggt gatcgatatt gggttaacca ccgtcaaagt
     9961 gcgtaactgg gacaatacca ttaccactat tcccacctgg tctctggttt ctgactcctt
    10021 taaaaactgg agcgggatgt cagcatctgg cgggcgacgt attaagcgca gtatcagtat
    10081 tgatgtcacc agtattcgtt ttcttgatga agacgaaatg caacgtctga ataaagcgca
    10141 cttgttaaaa ccttatttaa ccagccgcca tcaggaaatt aatgagtgga atcgccagca
    10201 aggttctacg gagtcgatat taaacctgcg ccgaatgacc aatattggaa cctttcgtgc
    10261 ctatctgaac gaatatctgc gtaaccatcc gcggattcgt aaagatatga ccttaatggt
    10321 acgccaactg gctccaggtg ataacggttt accgctcgag atctacgcgt ttaccaacac
    10381 cgtggtgtgg ctggaatatg aaagcattca ggctgatata ttcgatcaca tatttgcgat
    10441 tgtcgaagag tttggtctgc gacttcatca gtcgccagcc ggcaatgata ttcgctctct
    10501 ggcgggtgca tttaagcagt aattaaaaaa accgctctca tcgaatggat gagagcggac
    10561 atcgttttgt cggatgtagc gtgaatgcct tatttccgac gcagcgtttt aaatgccaca
    10621 aacaggaata caatccacac cggcagcagg atcgctgaca agcgcatatc atccatcgtg
    10681 cacatcagca gcaaaatcat gccgaggaag gcaatgcaaa gatagttgcc gaacggataa
    10741 agcagcgcct taaactgtgt ttcacgtccc tgacgtcgca tcgccgcgcg aaaacgcaga
    10801 tgcgccaggc agatcataat ccagttcaac agcagcgttg ctaccaccag cgccatcagc
    10861 agaccaaacg ctttttgcgg cagcagatag ttgattaaca ccaccagcga agtgatcgct
    10921 ccggaaagca tcagcgagtt aatcggcacg ccgcgatggc tgacgcgagt caaaaacttc
    10981 ggcgcattac cctgcacaga aaggccaaac agcatgcggc tgttagagta aaccccgctg
    11041 ttatacactg acagcgatgc taccagaatg acgaagttca gcgcagaagc tacctcgttg
    11101 ctgtcgagat tatggaaaat catcacaaac gggctactgt tggatttcac ttccacccac
    11161 ggatagagcg ccagtaaaac caccagtgaa cagatgtaaa acagcaggat gcgatacacc
    11221 acctgattta ccgcttttgg aatgcttttt tccggatcgc gcgcttcagc ggcagtaatc
    11281 ccaatcagct ccagaccgcc gaaggagaac ataattaccg ccagcgacaa aatcagccca
    11341 ttccagccgg tggcgaagaa accaccgtag cgccagaggt tgtcgatact ggctttctcg
    11401 ccgccgtgac cagaaaacag cagccacagg ccaaagccga tcataccgat gattgccagc
    11461 actttaatca acgcaaacca gaactcggtt tcgccatata agcgcacgtt caccaggtta
    11521 acggcgttga tgataataaa gaaggcggca gcccaaatcc acgttggaac atccgggaac
    11581 cagtactgca tatagatgcc cgcagcggtc agctctgcca ttcccaccag cacgaacatt
    11641 acccagtagt tccagccaga gaggaagccc gcaaacggtc cccagtattt ataggcaaag
    11701 tgggcaaatg aaccggatac cggctcttcg accaccatct cgccgagctg gcgcataatc
    11761 aggaaagcga tgatcccggc gacgccgtag cccagcaata cagccggacc cgccatctga
    11821 atcgccgggc caatgccaag aaacagacca gtaccaattg cgccacccag cgcaatcagt
    11881 tgaatatgac ggttatgtaa tccgcgatga agcgtcggct cttgatttga cgcagtatct
    11941 tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg tgtctttttt gttgaggggc
    12001 accttttaac atttagtgcc catcgtcgca agacacaatc cacacggtta aacggggtat
    12061 cctgctttta tttccgtacc cgatgtcggt gcagccacat cagcttatac gccgccggga
    12121 taataaacag cgacagcaaa ggcgcggtga tcatgccgcc aatcatcggt gcggcaatcc
    12181 ggctcatcac ctctgaacca gcccccgttc cccacagaat cggcagcaga ccggcgataa
    12241 tcaccgccac cgtcatcgct ttcgggcgca cgcgcagtac cgcgccgtga tacaacgcct
    12301 catccagctt ctgctcactg aatgtttgcg gattattcaa cgacggctcg gcctctatgg
    12361 cgtgacgtaa atacatcagc atcaccacgc caaattcggc ggcgcccccg gcaagggcaa
    12421 taaaaccggt gcccgttgcc acagaaagat gaaagcccat ccaccacagc aaccagatgc
    12481 cgcccaccag cgcaaacggt acgctgctga taatcagcaa cgcttcgccc acccgacgga
    12541 atgccagata caacagcacg aagatgatca tcagtgtcat cggcaccatg agtttcagct
    12601 tatggttggc gcgctccagt agctcgaact gcccggagaa tgccacgctg gtgcccggtt
    12661 ttaactgcac tttttcaact atcgcttttt gcaaatcgtg aaccaccgac accatgtcac
    12721 gatcgcgggc atcgatataa atccagctcg tcgggcgcgc attctcggtt ttcagcatcg
    12781 acggtccggt agagacttta acgtcggcca cgtctgccag ggtgatttgc tgcttcatcg
    12841 gcgtcaggat cggtagctgg cgcagtgcct gctttgcgga taacgcagat taatgggata
    12901 acgggcaatc ccttccaccg tttcgccaac catcgccccg cccaccgcag aagtcacaaa
    12961 caactgcaca tccgccaccg tcataccgta acgcgcggct ttttcacggt taatctcaac
    13021 gttgatatag cgcccacctt ccagacgctc ggcaagcgca gaagccacgc ctggcaccgt
    13081 tcgcgctact tcttcaattt gctcagccat cgcgtcgata tccgccagca cagtgccgga
    13141 aactttaatg ccgatggggc ttttaatgcc ggttgagagc atatcgatac ggttacgaat
    13201 tggcggcacc cacagattcg ccagccccgg cagccgcacg gtgttatcca gttcctcaat
    13261 gattttgtcc atcgtcatgc caggccgcca ctgatcctgc ggcttaagct ggatggtcgt
    13321 ttcaaccatc tcgagcggag cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa
    13381 tacccgcgcc acttcaggta cgctcataat tagcttgtcg tttttttgca gcatactcgc
    13441 cgcctctgct gcggaaatcc ccggcagcgt cgatggcata tacaacaagt cgccttcatt
    13501 gatctgcggt aaaaattccc cgccaacttt attgagcggc cagagaaccg tcagcaccga
    13561 aagcgctgcc accaacagcg tggttttcgg ccagtgcagt actttcagca atagaggatg
    13621 ataaacacga atcaaaaagc gattgagcgg gttactgctt tccggcggaa ttttgccacg
    13681 gatccagtaa cccatcagga tcgggatcac tacgatcgcc agcagcgccg cacccgccat
    13741 cgcatacgtt ttggtgaacg ccagcgggcc aaacagacgc ccttcttgcc cttccagggt
    13801 gaagattggg ataaacgaca gcgtgataat cagcagactg ataaacagcg ccggccccac
    13861 ttcaacagat gcatcggtaa tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg
    13921 atgctggtgc tgccactctt ccagccgttt atgcgcattc tcgatcatga cgatagcagc
    13981 atcgaccatc gccccgacgg caatcgcaat gccacccaac gacataatat tggcattcag
    14041 tccctggaag ggcatgacaa taaaagcaat acacaacccc agcggcaacg aaataatcgc
    14101 caccagcgcc gagcgcacat gccagagaaa cagcgcacag actaccgcca ccacaataaa
    14161 ctcttccagc aacttgccgc tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc
    14221 gtatgttgta actatctcca cgccttccgg cagactactt ttcagcgttt ccagtttgtc
    14281 cttcacggcg gcgatcactt ctcgggcgtt tttaccggag cgcaggatca ccaccccgcc
    14341 cgccacttcg ccttcgccgt tcagttcggc aatgccccgg cgcatctccg ggccaacctg
    14401 gaccttcgca acatcgcgca gataaacggg cacgccattt tcactggctt ttaaaacgat
    14461 gtgattaaag tcgtcgagcg tttgcagata gccgctggcg cgcaccatat attccgcttc
    14521 cgccagttcg atcgacgaac cgcccgcttc ctggtttgaa gcatccagcg cgctttttac
    14581 ttcggcgaga ctgatgccat actgcgccag gcgctgggga tcgataacca cctgatactc
    14641 tttcaccaca ccgcccaccg acgccacttc cgcaacgtca gggatggttt tcagctcata
    14701 tttgagaaac cagtcctgta atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc
    14761 caccagtgca tattcataga tccagccaac acccgtggca tctggcccca gctcggcgct
    14821 gactcccgca ggcagcttac cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc
    14881 ccagtacgga tcggtaccat cttcgaaaat gacatacaca taagagtcgc caaactgcga
    14941 gaaaccacgc acagtcttcg cgccaggcac cgacaacatg gtggtggtta gcggataagt
    15001 cacctgattt tcaacgattt gcggtgcctg cccgggatag ctggttttaa taatcacctg
    15061 cacatcggag agatccggca gcgcatccac tggcgtatta atgatggtcc aggtgcccca
    15121 gatgctaaga aacaacgcgc ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat
    15181 aatccattca atcattggtt attccctcaa tgcgcatggg tagcactttc agagcgcatc
    15241 cgctccagtg cgccagaaat attggcttcg gaatcaatca ggaaaaggcc gctggaaacc
    15301 accttttcac cttccgccag accagagcgt aatgcggtga caccttgcga tgcctggaaa
    15361 acggcaacgc gtttcggtac aaagcgcccg tcggcatcaa tggtaatcac ccgctgttcg
    15421 ctaccggtat caatcagccc ttgtgacgga atgagcagca tcggttcgct ggcggtattg
    15481 agttgcaacc aggcgttcat gcccggtttt agcgcctcgt cggcgttgtc gacttccaga
    15541 cgcagctgca gcgtgcgggt cgcggcatcc acgccgggta gcagcgtcca tttgcggatg
    15601 gtgagtgttt tatccggtcg tgccggaacg gtgagcgtaa actgcgaggc atctttcacc
    15661 agccaggcga tagactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt
    15721 ttcgctacca cgttatcttt ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc
    15781 acgccatcaa tgggcgcttt gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg
    15841 cggcgaatat ccgcctccgg cattcccgcc agtcgcagcc gctcaagaat gccttcagtc
    15901 tgggtcgccg taccgccggt ttcgcgcagc agtaaatact cactctgcgc ttccacccag
    15961 tcaggaatgg tcaggtcgag aagcggtgtg cccttttgta ctttatcgcc cacggtaagc
    16021 ggatacacct tgtcgataaa cccggcagcg cgagcctgca caatggcata ctgatactcg
    16081 ttgtaactga cattcgccgg gaaactctgg gcaaaagtca gcggtccgcg cgtgacggta
    16141 gccgttttca cccccagatt ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa
    16201 ctctcttcat cggcatattt cggcaccaga tccatatcca taaacggcga tttacctggt
    16261 ttatcgaacc gcgtattggg gtacatcggg tcgtaccaga ataagatttt acgttc
//
LOCUS       DAJVOY010000087        15813 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVOY010000087 DAJVOY010000000
VERSION     DAJVOY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.087"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1229
                     /locus_tag="OQL90_002423"
     CDS             <1..1229
                     /locus_tag="OQL90_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1369173.1"
                     /translation="LPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAGNLR
                     ALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNTMKK
                     TKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRTVDS
                     YIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQSQNL
                     WKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQP
                     ALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLG
                     ARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWI
                     DDMNDEQIAMLNARLS"
     gene            1281..2216
                     /gene="ttcA"
                     /locus_tag="OQL90_002424"
     CDS             1281..2216
                     /gene="ttcA"
                     /locus_tag="OQL90_002424"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1369174.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGIPHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2345..3718)
                     /gene="dbpA"
                     /locus_tag="OQL90_002425"
     CDS             complement(2345..3718)
                     /gene="dbpA"
                     /locus_tag="OQL90_002425"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1369175.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4164..5147)
                     /gene="zntB"
                     /locus_tag="OQL90_002426"
     CDS             complement(4164..5147)
                     /gene="zntB"
                     /locus_tag="OQL90_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1369176.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5402..6634
                     /gene="dgcM"
                     /locus_tag="OQL90_002427"
     CDS             5402..6634
                     /gene="dgcM"
                     /locus_tag="OQL90_002427"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCS1369177.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6655..7218)
                     /gene="smrA"
                     /locus_tag="OQL90_002428"
     CDS             complement(6655..7218)
                     /gene="smrA"
                     /locus_tag="OQL90_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS1369178.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7548..8456)
                     /locus_tag="OQL90_002429"
     CDS             complement(7548..8456)
                     /locus_tag="OQL90_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1369179.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8632..9932
                     /gene="abgA"
                     /locus_tag="OQL90_002430"
                     /pseudo
     CDS             8632..9932
                     /gene="abgA"
                     /locus_tag="OQL90_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            9932..11377
                     /gene="abgB"
                     /locus_tag="OQL90_002431"
     CDS             9932..11377
                     /gene="abgB"
                     /locus_tag="OQL90_002431"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCS1369180.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            11414..12940
                     /gene="abgT"
                     /locus_tag="OQL90_002432"
                     /pseudo
     CDS             11414..12940
                     /gene="abgT"
                     /locus_tag="OQL90_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12951..13466
                     /gene="ogt"
                     /locus_tag="OQL90_002433"
     CDS             12951..13466
                     /gene="ogt"
                     /locus_tag="OQL90_002433"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1369181.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13661..14413
                     /gene="fnr"
                     /locus_tag="OQL90_002434"
     CDS             13661..14413
                     /gene="fnr"
                     /locus_tag="OQL90_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1369182.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14565..15515
                     /gene="uspE"
                     /locus_tag="OQL90_002435"
     CDS             14565..15515
                     /gene="uspE"
                     /locus_tag="OQL90_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1369183.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15565..15813)
                     /locus_tag="OQL90_002436"
                     /pseudo
     CDS             complement(15565..15813)
                     /locus_tag="OQL90_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT         4090 a         3917 c         3894 g         3912 t
ORIGIN      
        1 aattaccaac aggtgtcgag attcgaggta aatacattcg catctggttc atgtttcgag
       61 gaaaacgatg tcgggaaaca ttgaaaggct gggagattac taacagtaac attaaaaagg
      121 ccgggaattt aagagcgttg atagttcatg aaatcagttc cggtgaattt gagtatttaa
      181 gacgttttcc ccagtccagc actggggcaa aaatggtgac aacgagggtc ataaaaacat
      241 tcggggagct ttgtgatatc tggacaaaaa ttaaagagac agagttaaca acaaacacaa
      301 tgaagaaaac gaaatcacaa ttaaaaacac tcaggataat aatttgtgag agtaccccga
      361 tatcgcatat tcgttatagc gatatcttaa actaccggaa tgaactgctg catggagaaa
      421 cgctttacct agataatcca agatccaaca aaaaaggaag aaccgtgcgc acagttgata
      481 gctatatcgc cctgctctgt tcgttgttac gttttgcgta tcagtcggga tttatatcaa
      541 ccaaaccatt tgaaggagta aaaaaattac agcgaaacag aataaaacct gacccgttat
      601 ctaaaacaga attcaatgca ttaatggaaa gtgaaaaagg acagagccaa aacttgtgga
      661 aatttgccgt ttactccggg cttcgtcacg gggaactggc agctctggcg tgggaggatg
      721 tggatttcga gaagggaatt gtgaatgtca gaagaaacct gacgatactt gatatgttcg
      781 gtcccccaaa aacaaatgcc gggatccgga cggtaacatt actgcagccg gctcttgaag
      841 cactgaagga gcaatacaaa ctgaccgggc atcatcgcaa aagcgaaatc actttttatc
      901 atcgggagta cggcagaact gaaaagcaaa aactgcattt tgttttcatg cccagggtgt
      961 gtaacgaaaa acagaaacct tattactcgg taagcagttt gggtgcaaga tggaatgcag
     1021 cagtaaaacg tgctggtatt cgccgccgta atccgtacca tacgcgacat acttttgcct
     1081 gctggctgtt gacggcagga gcgaacccgg catttatagc cagccagatg gggcatgaaa
     1141 ctgcgcaaat ggtgtatgaa atttacggta tgtggattga tgacatgaac gacgaacaga
     1201 tagccatgtt gaatgcgcgg ttatcgtagt tgcaaagttt gcccccaatt tgccccattt
     1261 agtaccagag aactgaaata atgcaagaaa atcaacaaat tacaaagaaa gaacaataca
     1321 gcctgaacaa attacaaaaa cgtctacgtc gtaacgtggg cgaagccatt gctgacttca
     1381 atatgattga agaaggcgat cgcatcatgg tttgcctctc agggggtaaa gacagctata
     1441 ccatgctgga gattctgcgc aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg
     1501 ctgttaacct cgatcaaaag caaccgggct tcccagaaca cgttctgccc gtgtatcttg
     1561 aaaagctggg cgttgagtac aagattgtcg aagagaacac ttacggtatc gtgaaagaga
     1621 agattccaga gggcaaaacc acttgctcac tgtgttctcg ccttcgtcgc ggtatccttt
     1681 atcgtaccgc aacggaactg ggggcgacga agatcgcgct gggtcaccat cgtgacgata
     1741 tcctgcagac gttgttctta aatatgttct acggcggcaa gatgaaaggt atgcctccga
     1801 aactgatgag cgatgatggc aaacatatcg ttattcgtcc gctggcctac tgccgcgaga
     1861 aagatattca gcgatttgcc gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg
     1921 gttcacagcc taacctgcaa cgtcaggtga ttgctgacat gttgcgtgac tgggataaac
     1981 gttatccagg gcgtatcgag acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc
     2041 tgtgcgatac caacctgttc gatttcaaag gcatccccca cggttctgaa gtggttaacg
     2101 ggggtgatct ggcgtttgat cgcgaagaga tcccactaca accggcgggc tggcagccag
     2161 aagaagatga aaatcagttg gatgagttac ggctgaatgt ggttgaagtg aaataaccag
     2221 gatagcgccc gatgcgcaag cgtatcgggc tactcttatg gaggccggat aagacgcggc
     2281 caacgtcgca tccggcaatg ccgaagaaga tgtttactct tgcacccggc aattcaacat
     2341 ttcattattt taataaccgc acccggcacg tttttccttt aatcttcccg ccctgtaact
     2401 gtttccatgc tttatgagca acagcctgac ggaccgcgac atagacatgc gccggatgca
     2461 cggcgatttt gccaatatct gcgccatcaa gcccgatatc tcctgtcagt gcacctaata
     2521 catcacccgg gcgcattttg gcttttttcc cgccatcgat acacaacgtt gccatttctg
     2581 cttccagcgg cacaatggaa ctattagctg gcggcgtttg ccagttaagt tttatctgca
     2641 acatgtcaga aatgatattg gcccgctgtg cttcttccgg agcacagaaa ctgatcgcca
     2701 gaccgctatt tcctgcacga gctgtacggc cgatgcgatg tacatgaact tcagggtccc
     2761 acgccagctc aaagttcacc accagctcaa gcgatttaat atccagacca cgcgcagcaa
     2821 catcagtcgc aaccagtaca cgggcgctac cgttagcaaa acgtaccagg gtctgatcgc
     2881 gatcgcgttg ctccaaatcg ccgtgtaatg acaatgcact ttgccctact tcattcagcg
     2941 cgtcgcagac agcctggcaa tcttttttgg tattgcaaaa caccacgcaa gaggatggct
     3001 gatgcaagct taataaccgt tgcaacagag gaattttgcc tttgctggat gtctcataaa
     3061 attgttgttc aatgggtggc aaagcatctg ttgagtcaat ttcaatcgcc aaaggatcgc
     3121 gttgcactcg tccgctgatt acggcgatgg cttccggcca ggttgccgaa aacagaagcg
     3181 tctgtcgaga tgcaggcgca aaacggatga catcatcaat ggcatcgcta aatcccatat
     3241 ccagcatgcg gtcggcctca tccatcacca aagtattcag cgcatccagt gataccgtgc
     3301 ctttttgcaa gtgatccagc aaacgacccg gcgttgccac gataatatgc ggcgcatgtt
     3361 gcaacgaatc acgctgcata ccgaacggtt gaccaccgca caacgtcaaa attttggtat
     3421 ttggcagaaa acgcgccagc cgacgcaatt cacctgccac ctgatccgcc agttcacgcg
     3481 taggacacag cactaaagcc tgggtttgaa atagcgacgc atcaatttgc tgtaacaagc
     3541 cgaggccaaa agccgccgtt ttgccgctgc cggttttcgc ctgcacgcga acatcttttc
     3601 cggcaaggat cgccggaagc gcggcggcct gcaccggcgt catagttaaa taacccaact
     3661 cattaaggtt cgtgagttgg gcgggaggca aaacattcag ggtagaaaaa gcggtcacaa
     3721 tctattctcg tggtcatcga cgcaaagtta gcaggcgcgt atcctcgcag atctacgcgg
     3781 gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga acaggatcgc
     3841 caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata tttccctccg
     3901 gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa aaaagccgac
     3961 tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa aaaggaagta
     4021 agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa gggagatatt
     4081 gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt tttgtataca
     4141 gacagccgga aaaattgctt ttgttacaac catttactac gatgcaacca taaagcaaca
     4201 ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg ccacccgccg
     4261 ccagggatcc caccaaggtt gacgccaaat aaccctgtca gaaaggtact gggtaaaaag
     4321 accattgcca tcaatgacat tgtataggta cgacgagcta aattttcctg catcacctga
     4381 gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc gtcaagaccg
     4441 cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct catccacggc
     4501 aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata gcgacgcatc
     4561 acgattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg ctgatcaagg
     4621 agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc gctggaatga
     4681 tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg acccgtgccc
     4741 tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg ggtcgaaaca
     4801 attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg ttcatcggtg
     4861 ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct gactcggggc
     4921 cgtgtgctct cgcctgccag cgcatcacgt acgttattgg gaagcagcgg tgttgtcgcc
     4981 agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg atgcgcttca
     5041 tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag catccaggca
     5101 aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt gcctccatca
     5161 tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct catcacccca
     5221 ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca aaatgctttt
     5281 ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat tatctgatca
     5341 tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata acgggatctc
     5401 aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct ggattgtttc
     5461 gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga tgcaagacct
     5521 cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag aactgccgaa
     5581 atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg ttcagcgtaa
     5641 agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag aaacagaacc
     5701 ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag ccagtcgctc
     5761 ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga ctaactctgc
     5821 accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta acctcgccgc
     5881 gctcaatttc tacggttata accatgaaac gatgtgccag aaacatacct gggaaattaa
     5941 tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac ccggcggtca
     6001 taaacctttg aattttgttc ataaactggc ggatggatct actcgtcatg tgcagaccta
     6061 tgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc atgatattac
     6121 tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg cgatgaccgg
     6181 attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc agcacctcgc
     6241 catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac acattaacga
     6301 tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca ccctcgaaag
     6361 ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg tcttattgct
     6421 accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgtg taagcgtggc
     6481 caaagtgagt atttcgggct taccacgttt taccgtcagc attggtgtgg cgcatcacga
     6541 aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt atcgggcgaa
     6601 aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc gagatcaacg
     6661 gctgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt tacgtagtgc
     6721 gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg cctgaacatc
     6781 atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat gcgatttatc
     6841 ttcccgccct ttaccatgaa taatcagcac attacgcaaa ccatccgcca gggcttggtg
     6901 aatgaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa gattcaggct
     6961 cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt gcaacccttc
     7021 ccgccgaaac tccagcggct gacttagcgg gatgatgtcg agaaatccgg tggtgaggaa
     7081 attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg ttggatgcca
     7141 gtggacatct gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa gaaacagcga
     7201 tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat gataaccaga
     7261 ccgggtcggt ccaacaacgt attaccaaaa attccagtaa taagttccaa atattgccga
     7321 tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa tgtaacttat
     7381 attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac cgttataaca
     7441 cagtcaccgg cgcagaggag acaatgccgg acttaagacg cggatgcact gctgtgtgta
     7501 ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta actttgcaga
     7561 tagccgcatt ctcgccggaa ttgcgtgatt aatgacgggg tcagtggtgt ctggcgacta
     7621 tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac cggcaacatc
     7681 accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat gctgagaaaa
     7741 tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac accgacctgt
     7801 ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact gccgtgtggc
     7861 gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc aatggcgggg
     7921 tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa agtaaattcg
     7981 tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc ctgacgcaat
     8041 tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg cggatgctgc
     8101 tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc ccccatgccg
     8161 atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg ggctgcgcgc
     8221 agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc agttaacgtc
     8281 acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccaa ctcctgaata
     8341 gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg aatgctgccc
     8401 tgacgagcca cttcaacaaa agcacgaatt tgatgaattt ttacctgaaa agccatgacg
     8461 ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa tgcagatgat
     8521 tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg ataagtaaaa
     8581 actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt tatggagtct
     8641 ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg tgattttcat
     8701 cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc ggaagaattg
     8761 cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag tagccggatg
     8821 ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca acagggtgcg
     8881 ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc caccctggat
     8941 accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct ggatctcagt
     9001 gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg taacgccgga
     9061 atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct agcgcatacc
     9121 cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca gcctgcagag
     9181 gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga tgttgattat
     9241 tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg cggcagtgat
     9301 aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc tcacgcaggc
     9361 gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac tcttgcactg
     9421 catgcaatcg ccccggagct tccagagtaa acgtgggcgt tatgcaggca ggaagcggtc
     9481 gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac acgcggggcc agcgacgtca
     9541 ttaatcaata tgtttttgac cgtgcacaac aagcgattca gggcgcagca accatgtatg
     9601 gtgtcggcgt tgaaactcgt ctgatgggtg cagctaccgc cagttctcct tcgccgcaat
     9661 gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg gggcaatcag gccattgaac
     9721 gtgttgaagc gcctgcgggt tccgaagatg ccacattaat gatggcccgc gtgcagcaac
     9781 atcaagggca agcctcctac gtggtgtttg gcacacagct ggcggcaggt catcacaacg
     9841 aaaaattcga ttttgacgag caggttctcg ctattgccgt cgaaacgctg gcgcgcaccg
     9901 cgctcaattt tccctggacg cgaggtatct gatgcaggaa atctatcgtt ttatcgacga
     9961 tgcgattgaa gccgatcgcc aacgttatac cgatattgcc gatcaaatct gggatcatcc
    10021 agaaacacgt tttgaagagt tctggtcagc ggagcatctg gcttcggcgc tggaatctgc
    10081 aggcttcacc gttacccgca acgtaggcta tatcccaaat gcctttattg cttcgtttgg
    10141 tcaaggcaaa ccggttatcg ccctgctggg agaatatgac gccctggcag gtttaagtca
    10201 gcaagcaggt tgcgcgcaac ctacatccgt gacgcccggt gaaaatggtc acggttgcgg
    10261 acacaatttg ctgggaaccg ccgcctttgc cgctgcaata gccgtcaaga aatggctgga
    10321 acaatatggg caaggcggca cggtgcgctt ttatggttgt cctggcgaag aaggcggctc
    10381 gggtaaaacg ttcatggttc gcgagggggt atttgatgat gtggatgcgg cactcacttg
    10441 gcacccggaa gcctttgccg gtatgttcaa tacccgcacg ctggcaaaca ttcaggcatc
    10501 atggcgcttt aaagggatcg cagcacatgc cgcgaatttc cctcatttgg gacgcagcgc
    10561 ccttgatgcc gtaacgttga tgaccactgg caccaacttc ctcaacgaac atattattga
    10621 aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg atctcgccca acgtggtcca
    10681 ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa atgaccgacg tgcagcatat
    10741 ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg atgaccgaaa ccacggttga
    10801 atgccgcttc gacaaagcct gttccagtta tctcccgaat cgcaccttag aaaatgccat
    10861 gtaccatgcc ctatcccatt ttggtacccc ggaatggaac tccgaagaac tggcttttac
    10921 gaaacaaatt caggctacgc tcccccccaa cgatcggcaa aacagtctga ataatatcgc
    10981 cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt catcgtgaaa cggtactggc
    11041 gaatgaggtc gctccatatg ccgccaccga taacgtgctt gcggcatcga ctgatgtcgg
    11101 cgacgtcagt tggaaactgc ctgttgccca gtgtttcagc ccctgttttg ccgtcggtac
    11161 accgctacat acgtggcaac tggttagcca ggggcgaaca tctattgctc ataaaggaat
    11221 gctgctggcg gcgaaaacta tggcagcaac cacactcaat ctcttcattg attcagggct
    11281 attgcaagaa tgccaacaag agcatcagca agttacggac acgcaaccgt atcactgccc
    11341 tatcccaaaa aacgtgacac tgtcatcttt aaaataacaa caacaacgca aacacaacaa
    11401 ccgaggaatg cccatgagta tgtcatccat accgtcgtcc tcctaatccg ggaagctcta
    11461 tggctgggtc gaaagaattg gtaacaaggt tccccatcct tttttgctct ttatctattt
    11521 gattatcgta ctcatggtga cgacggcaat tttgtcggcc tttggcgtca gtgcgaaaaa
    11581 cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc agtgtggaag gattacactg
    11641 gtttttaccc aatgttatta aaaactttag cggttttgct ccacttggtg cgatcctggc
    11701 gctggtttta ggtgccggtc tggcggagcg cgtcggctta ctgccagcgc taatggttaa
    11761 aatggcatcg catgttaatg tccgctacgc cagttatatg gtgctgttta ttgctttttt
    11821 cagccacatt tcttccgatg cggcgttagt gatcatgcca ccgatgggtg cgctgatttt
    11881 tctggcggtg ggcaggcatc cagttgcagg tttactggct gccattgcag gcgtaggtta
    11941 cggctttacg gctaatttac tgattgtcac aaccgacgtg ttgctgtcgg ggatccgcac
    12001 ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt gtaattgata actggtattt
    12061 tatggccagc tccgtagtcg tactgacgat tgttggcggc ctgataaccg acaaaatcat
    12121 cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag aaactgcaga cattgaccga
    12181 aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg ctacttttta ttgctgcgat
    12241 cgcgctgatg gtgatcccgg aaaacgggat attgcgcgat ccgattaatc acaccgtgat
    12301 gccatcaccc tttattaaag gtatcgtgcc actaatcatt ctttttttct ttgtggtttc
    12361 gctggcttat ggcatcgcta cccgcacaat tcgacatcag gcggatttac cgcatttaat
    12421 gattgaaccg atgaaagaga tggcgggatt tatcgtgatg gtttttcccc tcgcccagtt
    12481 tgtcgccatg tttaactgga gcaacatggg gaaattcatc gccgtggggc tgaccgatat
    12541 actggaaagt tcagggctta gcggcatccc ggcgtttgtc ggtctggcgt tgctttcctc
    12601 tttcttatgc atgtttattg ccagtggttc cgcaatctgg tcgattctgg cccccatttt
    12661 cgtaccaatg tttatgctac ttggctttca cccggcattt gcgcaaatcc tctttcgtat
    12721 tgccgactca tccgtattgc ctttagcgcc ggtatctcct tttgttccac tgtttcttgg
    12781 attcctgcaa cgctacaaac cagacgcgaa actgggtact tactattcgt tagtcttgcc
    12841 ctatccgctt atctttttgg tggtatggct gctgatgttg ctggcgtggt atcttgttgg
    12901 tctgccgata ggtccgggga tttacccacg tttgtcttaa gagagaacgg atgctgagat
    12961 tacttgaaga aaaaattgcc acgccactgg gtccactgtg ggtgatttgc gatgagcaat
    13021 ttcgcctgcg ggcggttgaa tgggaagagt acagcgaacg catggtgcag ctgctggaca
    13081 tccattatcg caaagaaggc tatgagcgca tttctgccac caacccaggc ggtttaagcg
    13141 acaagcttag tgattatttt gccggtaatc ttagcattat tgatacgctt cccactgcca
    13201 caggggggac gccatttcag cgcgaagtct ggaaaactct acgcactatc ccctgcgggc
    13261 aggtaatgca ttacggccaa ctggctgaac aattgggccg ccctggcgcg gcgcgtgccg
    13321 ttggtgcggc aaacggatcg aatcccatca gcatcgtcgt accttgccat cgggttattg
    13381 gccgaaacgg caccatgacc ggatatgcag gcggagttca gcgaaaagag tggttattgc
    13441 gccacgaagg ttatcttttg ctgtaaacat taaacaattt gtgccagctt gttcacactt
    13501 ttatgtaaag ttacccttaa caacttaatg gttttcaaat agatagacat atatttacat
    13561 ctaatatcgg aattctctgc tgttaaggtt tgcttagact tacttgctcc ctaaaaagat
    13621 gttaaaattg acaaatatca attacggctt gagcagacct atgatcccgg aaaagcgaat
    13681 tatacggcgc attcagtctg gcggttgtgc tatccattgc caggattgca gcatcagcca
    13741 gctttgcatc ccgttcacac tcaacgaaca tgagcttgat cagcttgata atatcattga
    13801 gcggaagaag cctattcaga aaggccagac gctgtttaag gctggtgatg aacttaaatc
    13861 gctttatgcc atccgctccg gtacgattaa aagttatacc atcactgagc aaggcgacga
    13921 gcaaatcact ggtttccatt tagctggcga cctggtggga tttgacgcca tcggcagcgg
    13981 ccatcacccg agcttcgctc aggcgctgga aacctcgatg gtatgtgaaa tcccgttcga
    14041 aacgctggac gatttgtccg gtaaaatgcc gaatctgcgt cagcagatga tgcgtctgat
    14101 gagcggtgaa atcaaaggcg atcaggacat gatcctgctg ttgtcgaaga aaaatgccga
    14161 ggaacgtctg gctgcattca tctacaacct gtcccgtcgt tttgcccaac gcggcttctc
    14221 ccctcgtgaa ttccgcctga cgatgactcg tggcgatatc ggtaactatc tgggcctgac
    14281 ggtagaaacc atcagccgtc tgctaggtcg cttccagaaa agcggtatgc tggcagtcaa
    14341 aggtaaatac atcaccatcg aaaataacga tgcgctggcc cagcttgctg gtcatacgcg
    14401 taatgttgcc tgatttttcc gcataactca ctatccttct gtcatatcat taaatttttc
    14461 tgatttattg atctggcaga aggttcatca ctgtttcatt caccagatat gggttaatct
    14521 tttaattaca aactgcgttg acagttgttg taaggagacc ctgtatggct atgtatcaga
    14581 acatgctcgt tgttatcgat cctaaccagg acgaccaacc agcattgcgg cgagctgttt
    14641 atttacatca acggattggt ggcaaaatta aagccttttt gccgatctat gacttctcat
    14701 acgaaatgac caccctgctc tccccggacg aacgtaccgc tatgcgtcag ggcgtcatca
    14761 gccagcgtac agcctggatc cacgagcagg caaaatatta tctcaatgct ggcgttccca
    14821 ttgaaattaa agtggtctgg cataaccgtc ctttcgaagc catcattcag gaagtgatca
    14881 gcggcgggca cgatttggtg ctaaaaatgg cgcaccaaca tgaccgtctg gaagcggtga
    14941 tttttacgcc aacggactgg catctgttac gcaaatgccc aagcccggtg tggatggtga
    15001 aagaccagcc gtggccggaa ggaggtaagg cgctggtggc ggtgaatctc gccagtgaag
    15061 agccgtatca taatgcgctc aatgaaaaac tggtcaaaga gacgatcgaa ctggcagaac
    15121 aagtcaacca taccgaagtt catctggttg gcgcttaccc agtaacgcca attaatatcg
    15181 cgattgaact gccggaattt gagccgagcg tttataacga tgccattcgt gggcaacatt
    15241 tgctggcaat gaaagccctg cggcagaaat tcggcattaa tgaaaacatg acgcacgtag
    15301 aaaaaggtct gcccgaagag gttattcctg atttggcgga gcacttacag gcggggattg
    15361 tggttctggg cacggtagga cgcaccggta tttcagcagc attcctcggc aacacggcgg
    15421 aacaggtgat tgatcatctt cgctgcgatc tgttggtcat taaacctgac cagtatcaga
    15481 cacccgttga actggatgac gaagaagacg attaacgact cttcaataac tgacaaggcc
    15541 cgagttatcg ggcctatttt ttacttactg ctcttcgccg ccaaggaaat aaattcccaa
    15601 cgggattgcc agcaacacag taaagaccag gctataaaca aaaatcatcg atgactgaat
    15661 cacatacatt gatgtcgtcc actcagacag cggaatattg tactgttcta taacgccgcc
    15721 gatagtcgcg cgagtcacaa ccgacgccgc aataatgaca aaccgccaac aaaccaaaga
    15781 gaaatttctt ccctttcgct gacttcagtt tcg
//
LOCUS       DAJVOY010000088        15611 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVOY010000088 DAJVOY010000000
VERSION     DAJVOY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.088"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..643
                     /gene="cobS"
                     /locus_tag="OQL90_002437"
     CDS             <1..643
                     /gene="cobS"
                     /locus_tag="OQL90_002437"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1369184.1"
                     /translation="ITFPLIGLLLGAISGLVFMVLQAWCGVPLAALFSVLVLALMTGG
                     FHLDGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFVVLAKILVLSELALRGE
                     PILALLAAACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAV
                     LLPGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            655..1734
                     /gene="cobT"
                     /locus_tag="OQL90_002438"
     CDS             655..1734
                     /gene="cobT"
                     /locus_tag="OQL90_002438"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1369185.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            1796..2731
                     /gene="ldtA"
                     /locus_tag="OQL90_002439"
     CDS             1796..2731
                     /gene="ldtA"
                     /locus_tag="OQL90_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1369186.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(2787..2862)
                     /locus_tag="OQL90_002440"
     tRNA            complement(2787..2862)
                     /locus_tag="OQL90_002440"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2827..2829),aa:Asn,seq:gtt)
     gene            3189..3560
                     /locus_tag="OQL90_002441"
                     /pseudo
     CDS             3189..3560
                     /locus_tag="OQL90_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3559..3993
                     /locus_tag="OQL90_002442"
                     /pseudo
     CDS             3559..3993
                     /locus_tag="OQL90_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            4336..5052
                     /locus_tag="OQL90_002443"
     CDS             4336..5052
                     /locus_tag="OQL90_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1369187.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(5508..5583)
                     /locus_tag="OQL90_002444"
     tRNA            complement(5508..5583)
                     /locus_tag="OQL90_002444"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5548..5550),aa:Asn,seq:gtt)
     gene            complement(5684..7327)
                     /gene="yeeO"
                     /locus_tag="OQL90_002445"
     CDS             complement(5684..7327)
                     /gene="yeeO"
                     /locus_tag="OQL90_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCS1369188.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            7332..7407
                     /locus_tag="OQL90_002446"
     tRNA            7332..7407
                     /locus_tag="OQL90_002446"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7365..7367,aa:Asn,seq:gtt)
     gene            7569..8831
                     /locus_tag="OQL90_002447"
     CDS             7569..8831
                     /locus_tag="OQL90_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1369189.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(9543..10427)
                     /locus_tag="OQL90_002448"
     CDS             complement(9543..10427)
                     /locus_tag="OQL90_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HCS1369190.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRYVLETARAKDPGMAAALELSRLMGLRSQEVVQSAQSLR
                     TWKQSLERGEPRLTVVFGTKGGRPRETVILDAVAIRKALDNALVVAEDRHGRLIDKPD
                     LKSAMKYWHSQASRLGLTGTYSPHSLRYAWAQDAIRHYLAQGFSEKEALAMTAMDLGH
                     GDGRGRYVAQVYGRKDTD"
     gene            10986..11132
                     /locus_tag="OQL90_002449"
     CDS             10986..11132
                     /locus_tag="OQL90_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369191.1"
                     /translation="MYRIKTLASVAMFCDPVFAEVCTGKRIDRPDNRRFFQVVKRWAL
                     STTA"
     gene            complement(11292..11609)
                     /locus_tag="OQL90_002450"
     CDS             complement(11292..11609)
                     /locus_tag="OQL90_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1369192.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(11610..11867)
                     /locus_tag="OQL90_002451"
     CDS             complement(11610..11867)
                     /locus_tag="OQL90_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCS1369193.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(12216..12422)
                     /locus_tag="OQL90_002452"
     CDS             complement(12216..12422)
                     /locus_tag="OQL90_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1369194.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(12419..12880)
                     /locus_tag="OQL90_002453"
     CDS             complement(12419..12880)
                     /locus_tag="OQL90_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369195.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(13251..14855)
                     /locus_tag="OQL90_002454"
     CDS             complement(13251..14855)
                     /locus_tag="OQL90_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369196.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(14868..15233)
                     /locus_tag="OQL90_002455"
     CDS             complement(14868..15233)
                     /locus_tag="OQL90_002455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369197.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
     gene            complement(15261..15470)
                     /locus_tag="OQL90_002456"
     CDS             complement(15261..15470)
                     /locus_tag="OQL90_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369198.1"
                     /translation="MNKTPANIMDLYSLLLDHIRDISNRIANENTDIYKQFWNEDSYG
                     RTLTPKPEESCRNIFLDLLRVRLNP"
BASE COUNT         4138 a         3729 c         3701 g         4043 t
ORIGIN      
        1 tattactttt cctttaattg gattattgct tggcgcgatt agcgggctgg tcttcatggt
       61 gctgcaggca tggtgtggcg taccactggc ggcactgttt agcgtacttg tgctggcgct
      121 gatgaccggg ggattccacc tggacggcct cgccgatacc tgtgatggcg tattttctgc
      181 acgtagccgc gatcgcatgt tggaaatcat gcgtgatagc cgtttaggca cccacggcgg
      241 tctggcattg atttttgttg tactggcaaa gattctggta ttaagcgagt tggctctgcg
      301 tggcgaaccg atccttgcat tgctggcggc agcatgtgcg gtcagccgcg ggactgccgc
      361 attattgatg tatcgtcatc gttatgcccg ggaagaagga cttggcaatg tgttcattgg
      421 caaaattgat gggcgacaaa cctgcgtcac actcggctta gccgctattt ttgctgccgt
      481 attattgccg ggtatgcatg gtgtggctgc tatggtggtg acgatggtgg cgattttcat
      541 cctcggtcag cttctcaaac gtacgctggg cgggcaaacc ggcgatacgc tgggagctgc
      601 tatagagctt ggtgaactgg tcttcctgct ggcactgcta taaagagacg tgacattcaa
      661 acacttgccg atttactgaa tacgatccct gctatcgatc ctgccgctat gtcgcgtgca
      721 caacggcata tcgacgggtt actcaaacct gttggtagcc tgggaaggct ggaggcgctt
      781 gccatacaac tggcaggaat gccggggttg aatggcgttc cgcatgtggg caaaaaagcg
      841 gtactggtca tgtgtgccga tcacggcgtc tgggaggaag ggggcgctat ttccccaaaa
      901 gaagtgacag ccattcaggc tgaaaatatg acccgcggaa caaccggcgt gtgtgtgctg
      961 gcagcacaag cgggcgctaa cgtctacgta attgatgttg gtattgatac tgctgagcct
     1021 atccccgggc ttatcaacat gcgtgttgct cgaggtagtg gcaatattgc ttcagctccg
     1081 gcaatgagtc gccatcaggc agaaaagttg cttttggacg tcatatgtta tacgcgggag
     1141 ctggcaaaaa acggtgttac gctgtttggt gtaggtgaac tggggatggc aaacacgaca
     1201 ccggcagcgg caatagtcag cacaatcact ggccgggctc ctgaagaagt ggttgggatt
     1261 ggcgcaaacc tgccgacaga taaactggct aataaaattg atgttgtgcg ccgggcgatt
     1321 acgttgaatc aaccaaatcc gcaggatggc gttgatgtcc tggcaaaagt gggtggattt
     1381 gatttggtcg gaatggctgg cgtgatgtta ggcgctgcgt cctgcggttt acccgtgttg
     1441 ctggatggat ttctttctta tgctgctgcg ctcgcagcct gtcagatgtc tcctgcaatc
     1501 aaaccgtatc tcattccttc tcacctgtcg gcagaaaaag gagcgcgtat agcgctgtcg
     1561 catttggggc tggagcctta tctcaatatg gagatgcgtt taggtgaggg gagtggcgca
     1621 gctctggcga tgcccatcat cgaagctgcc tgtgcgatat ataacaacat gggcgaactt
     1681 gctgccagta atattgttct accggggaat acgacttctg atttgaacag ttaaaaacga
     1741 aaagtctgat aaaagctata cttaacggat agctttcgcg acttaggaaa gggacatgat
     1801 gcgtcgtgta aatatttttt gctcatttgc tctgcttttt gccagccata ctagcctggc
     1861 ggtaacttac ccattacctc cagagggtag ccgtttagtg gggcagtcgt atactgtaac
     1921 tgttcctgat cacaataccc agccgctgga gacttttgcc gcacaaaacg ggcaagggtt
     1981 aagtaacatg ctggaagcga acccgggtgc tgatgttttc ttgccgaagt ccggctcgca
     2041 actcaccatt ccgcagcaac tgattttgcc cgccactgtt cgtaaaggga ttgtggttaa
     2101 cgtcgctgag atgcgtcttt attactaccc accagacagt aatactgtgg aagtctttcc
     2161 tattggtatc ggccaggctg ggcgagaaac cccgcgtaac tgggtgacta ccgttgaacg
     2221 taaacaagaa gctccaacct ggacgccaac gccgaacact cggcgcgaat atgcgaaacg
     2281 aggggagagt ttgcccgcat ttgttcctgc gggccccgat aatcccatgg ggctgtacgc
     2341 gatttatatt ggcaggttgt atgccatcca tggtaccaat gccaattttg gtattgggct
     2401 acgggtaagt cagggctgta ttcgtctgcg caatgacgat atcaaatatc tgtttgataa
     2461 tgttcctgtt gggacgcgtg tgcaaattat cgaccagcca gtaaaataca ccactgaacc
     2521 agatggctcg aactggctgg aagttcatga gccgttgtcg cgcaatcgtg cagaatatga
     2581 gtttgaccga aaagtgccat tgccggtaac cccatctttg cgggcgttta tcagcgggca
     2641 agaagttgat gtaaatcgcg caaatgctgc gttgcaacgt cgatcgggaa tgcctgtgcc
     2701 aattagttct ggttcaagac agatgtttta agagcgttgg taattagaaa gcaaaaagcc
     2761 tgctagaaag caggcttttt tgaatttggc tcctctgact ggactcgaac cagtgacata
     2821 cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgtg aacggggcgc
     2881 atattaatgg cagtgcatgg tgatgtcaaa gggtgaaatg catttttttg tttgtttgct
     2941 gtcaatttca acaaagtggt gagctttcaa tcttattggg taatgaacca tcgtggtgca
     3001 taccctcctt ttatagggca ggggaacgcg acagctgatt aaaggagcaa atgacgaatc
     3061 tatcataatc tcttatttga cagttggtta gcttgtacat caacgccaaa ataaaactgg
     3121 caagcatctt gcaatctggt tgtaagtaat ggcggcactt gggccgattc ttaaaaaccg
     3181 gaggcaacat gaacttcaga cgcctgaaat acttcgtaaa aattgtagat attggtagcc
     3241 tgacccaggc tgctgaagta ttgcatatcg cacaaccagc gctcagccag caggttgcca
     3301 cactggaagg tgagttaaat caacaacttt tgatccgtac aaagcggggc gttacgccaa
     3361 cagacgccgg aaaaattctc tatacccatg cgcgggccat tttacgtcag tgtgaacagg
     3421 cccaactggc ggtgcataac gttggtcagg cattatcggg gcaagtctcg attggctttg
     3481 caccaggaac cgctgcgtca tccatcacca tgcccttatt acaggcggtt cgcgctgaat
     3541 ttccggagat cgttatctat cccgagcatt gctgaagtgc aacgtgcgct ttatgacgcc
     3601 accaagctgg tgagcggcag gcccggtgag gaagtcaaac agcggctacg tactggtact
     3661 gtggtaacca cagatgacag gaactgggaa ttacgttact cagcttctgc acttcgtttt
     3721 aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc gcaaggatat
     3781 cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt gcatggcgag
     3841 attaaacttc ccggccaggc taaccgtttt tatgaaggcg ctatttccga acatctgcaa
     3901 attggcattc gggcgatcga tttgctgcgc gcagaaggcg accgactgca ttcgcgtaaa
     3961 ttacgaacct ttaatgagcc gccgttccga taataagttc tgaaccttct tatcagaaat
     4021 agatgtgaag gagtaagtaa gacacctggc aaatagcctg caataatcgt gggctgtttg
     4081 cttccttggg cggatacgtg ttttattatc gtcttaatga tttccacata ttaaaagcaa
     4141 gtatgctttc aaaacacaat tataaaaaat cccgccaaca atataagttt ttataaaatt
     4201 aaatataaga ttatggcttt agaatatttt tatttctaat agacgagatt tttcctgtta
     4261 tgatataata tgccgaatta acacatgtta acgatttacc agtaatgtaa ataaattttc
     4321 gaggagatca ttccagtggg acgtaaatgg gccaatattg ttgctaaaaa aacggctaaa
     4381 gacggtgcaa cgtctaaaat ttatgcaaaa ttcggtgtag aaatctatgc tgctgctaaa
     4441 caaggtgaac ccgatccaga attaaacaca tctttaaaat tcgttattga acgtgcaaag
     4501 caggcacaag ttccaaagca cgttattgat aaagcaattg ataaagccaa aggcggcgga
     4561 gatgaaacgt tcgtgcaggg acgttatgaa ggctttggtc ctaatggctc aatgattatc
     4621 gccgagacat tgacttcaaa tgttaaccgt acgattgcta acgttcgcac aattttcaat
     4681 aaaaaaggcg gcaatatcgg agcggcaggt tctgtcagct atatgtttga caatactggt
     4741 gtgattgtat ttaaaggatc agaccctgac catatttttg aaattttact tgaagctgaa
     4801 gttgatgttc gtgatgtgac tgaagaagaa ggtaacattg ttatttatac cgaacctact
     4861 gaccttcata aaggaatcgc ggctctaaaa gcagctggaa tcactgagtt ctcaacaaca
     4921 gaattagaaa tgattgctca atctgaagtt gagctttccc cagaagattt agaaatcttt
     4981 gaagggcttg ttgatgccct tgaagatgac gacgatgtac aaaaagttta tcataacgtc
     5041 gcaaatctct aattatatat taaagaaatc tgtctttatg gcagatttct ttaatctcat
     5101 ataatcctta taaaaaatat aatgttcaac tcgtcatatt gcttataaaa aatcctcgtt
     5161 cccagagaaa taatatttat tataattcca gttcttcttt ttctgattac agaaggcaaa
     5221 gtgacaatta cgcatagttt cccgataaag acgcgacagc tacatcccgc ataaggcatt
     5281 tttctcttta tccttgtacg gtacttcatg gaacagagtt tttgaccttg cgaatcgtga
     5341 tgtctgttgg ggaggggcaa tttgctcact gaagcgtgag actcgattaa gcgcacgaaa
     5401 cacagaaatc aaaaaacccg ggcacttttt tacaaggtaa ccgggtaaaa ataattttta
     5461 ttttttaact gttttgagac tcatagagat gtctcaaaac taaaatttgg ctcctctgac
     5521 tggacttgaa ccagtgacat acggattaac agtccgccgt tctaccgact gaactacaga
     5581 ggaatcgtga gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgttat
     5641 ttaaatttga aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg
     5701 gctttttttc acacttttgc ggttcggatc gagggtattt ccacagccaa cgtccagtaa
     5761 ccattcgcca gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga
     5821 caccaaccac accccagcca agcatgatgc ccagcacata accgacgaca acccggcaac
     5881 cccacatgct caacatagaa acccacatgg cgtaacgggc atcgcgagca cctttaaatc
     5941 cagcgggtag cccccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca
     6001 gaatcacaac gacatgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg
     6061 caaagggagc cgttagccag gcgatggccg ttaatccaag agtggaaagc cagaacacat
     6121 gccgcaactg aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga
     6181 tcgtagaagc agagccgagc gcacttccgg gtaagttgat aagagccgca attgaaaacg
     6241 cgataaaatt tccggcaata acactggtcc ccatcccggc aacgaacatt tgggttaata
     6301 accgaccact ggtaaataac actgattcga cactcgcggg aataccaatc cccatgactt
     6361 cccagataat gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag
     6421 gattaaaacc aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg
     6481 ttaaacccag ccctgccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca
     6541 atatgccgct aataataata ttaagaatat tcaggctacc gttaatcaat agcggtattt
     6601 tcgtattccc tgcaccacga agtgccccgc taccaataag agtgatggca gctgctggat
     6661 aactgagtac cgtcagctcc agataagtca acgccagtac tttaacttct gtcgtggcat
     6721 cacccgcgac gaaatcaata atttgttcgc caaaatgatg aataagcgtt gccaacagta
     6781 cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat
     6841 cccgcttacc gagactaaat gccacaacga cagtagtacc aagatcgata gcagcaaaaa
     6901 aagccataat gaccatattg aagctgtccg ccaatcccac gccggccatc gcatcttttc
     6961 ccagccagct gaccagaaaa gtgctcagaa cccccatcaa caggacacag gcattctcca
     7021 tgaagatagg aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc
     7081 gtttagcgtg ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag
     7141 ccgaccttaa ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa tcagcaaagc
     7201 tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca
     7261 aacagttggg gaatttaaaa atcgggtttg acaaaagatt tttcgccgtt aagatgtgcc
     7321 tcaacagcga ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca
     7381 ctggttcgag tccagtcaga ggagccaaat tccgattctt acgctatctt attagtcctt
     7441 atttgatatt gattcaataa gttagcgtga aaatctttcc agatgtgtat tcagttttcc
     7501 ctccgcatcc ggttaatttg gtggtcagat ttgggggcag gttggttcga ttctggagtg
     7561 acccccaaat gtctttaaac gattcaaaaa tccgtaactt aaaaccctct tcaaaacccg
     7621 taaaactgtc tgattcgcac ggtctgtatc tgctggtcaa tcagggcggt tcgcgtatct
     7681 ggtatctcaa gtatcgcttc agtggtaaag agtcccgcgt cagtctgggc gcatatcctc
     7741 tggtttcact tgctgaagca agacaacgac gtgacgatat ccgtaagcta ctgacgcaga
     7801 atattaaccc cgcccaccag cgcatgtctg ataaagccgc agcgtcaccg gaaaagtatt
     7861 ttaaagccgt ggcgcttgca tggcacaaaa ccaacaaaaa atggtcgcct gattatgccg
     7921 cgcgcatcct cgccagcatg gaaaatcata tctttccggc gattggtcat ctgcctgtca
     7981 ctacgctgaa aacgcagaat ttcactgcgt tactgcgggt tattgaggat aaaggttttc
     8041 tggaagtcgc gtcccgaacc cggcagcaac tctgcaacat catgcgttac gccgtacagc
     8101 agggattcac cgaaaacaac ctggcgcagc atctggaagg cgtcaccgcg ccgccggtta
     8161 agaatcacta tcccgccctg ccgctggagc ggttgcccga attgtttgag cgcattggcg
     8221 actatcagca gggacggcag cttacgaggc tggcggtggt gttgactctc cacctgttta
     8281 tccgttccag cgagctgcgc tttgcccgct ggggtgagat agatttcaga aacaaaatct
     8341 ggaccatccc cgccacaagg gaagcgattg agaaagtccg tttctctggc cgtggcgcaa
     8401 aaatgcgtac tccatatata gtgccgcttt ctcgtcaggc tatcgccatt ctgaagcaga
     8461 taaaggaaat ctccggccat ctggaactgg tgttccccgg cgaccataac ccgtataagc
     8521 cgatgagcga aaacactgtc aaccgggcgt tgcgtcttat gggctatgat accaaaactg
     8581 atgtctgtgg gcatggattc cggacaatgg cgtgtagcgc attagtggaa actgaactct
     8641 ggtcacggga taccgtggag cggcagatga gtcatcagga gcgcaacagc gttcgggcag
     8701 cgtatatcca ccgggctgaa catctcgacg cccgtacagc catgatgcag tggtggtcgg
     8761 attatctgga tgtaagtcgc gaggggtacg ttgcgccgta tatttatgcg cggcggcata
     8821 aggcggcctg agacagtggc tatataattt gttaaaaatg aaaaagcaga tcctgattga
     8881 atccggatct gctttttaca ttaggcaatc gctgccagac aatctgcacc agaaacgcca
     8941 taactgcacc agttcagtac caccgcttac ctgcgccagt ctgccgtcgc ctgtctgtgt
     9001 caggactgcg ccagatctta tcgaataaac atcaaactgc gccagtgtta cggccctttc
     9061 agccctttgg ccttttccgg tctggcgctg ttacccttag ccgcccctta ccctttccgg
     9121 cctttttcct ttgctggcgc aatcgccaga aaaatcgtca agctgcacca gaagcctgcc
     9181 aataggtcat accgttcaga agcatcgtta ttgagaggaa accgtacaaa aaatttctac
     9241 gtagaaattt tttcggtgga taacgatgaa taaagagtat tttataatgt gatggctatc
     9301 aaaaatcgac catccaaaaa taattcattg atttataatg aataaccatg aatttaatag
     9361 tcgatgatta ttgtttttat cagtattgat aggaaagtat acaaaaaatt ttggccccaa
     9421 aattttttgt acgaaaaccg atgaataacg cgaaattaat aggaaaccat ccaaaattat
     9481 ttggggcaaa taattttgcc cgaaaaccga tggataactc gatcttttca aagcaacggc
     9541 tatcaatcag tatcctttct tccgtaaacc tgcgccacat accgcccgcg cccgtcgccg
     9601 tgtcccaaat ccatcgccgt cattgccagc gcttctttct cactgaatcc ctgcgccaga
     9661 taatgacgaa tggcatcctg cgcccacgca tagcgcaacg agtgcgggga atatgtgcca
     9721 gttaacccga gacgtgacgc ctggctgtgc cagtatttca tcgcgctttt caggtcgggt
     9781 ttatctatca gcctgccgtg gcgatcttct gccacaacca acgcattatc cagcgctttt
     9841 ctgatagcga cagcatccag aataacggtt tctcgcggac gcccgccttt ggtgccgaac
     9901 accacggtta aacggggctc accccgctcc agcgattgct tccacgttct taatgactgc
     9961 gcactttgca ccacctcctg cgaacgtaat cccatcagcc ttgagagttc cagcgccgct
    10021 gccatccccg gatcttttgc gcgggcggtt tccagcacat agcggtaatg ctccggcgtg
    10081 atggcccgct tcgtaccgtt acgggatgcg ccagacaggc ccagcgagcg gttattcagt
    10141 cgctcactct gcgccagcct gtcacgcccg gcctgtttca gaatgcagcg caccgccgcc
    10201 atctcatttt gcagggaacg cttcgtgata ccctgcgcca gtcgttcacg aacatatccc
    10261 tcaatatacc gcgccttaag ctgctccacc cggcggatct gcacattctg tgccagaacc
    10321 agacgctcac aaaaccgctg caccagctta atacggtcgt gaacggtctt atggctgccg
    10381 cccgcctgtt gtgccaatcg cttcatttcc tgctccagaa tccccataca ttcccccttt
    10441 catgattgcc gcaaactcat ccccaaaaac tcaacactga tacagcgttc cccggcgcac
    10501 aggccgatag cccctgagtt ttgtcggggc tgcgaacgat acctgagcgc cggggcggca
    10561 gccgttgagg tgtggtgggg cttcggttgt gctctcagag cagccgcccg cttgcgcggg
    10621 tcatcccccg gatcgtccag atccgcctcg gtatcggttc atattctcct tatgggtaaa
    10681 tggcctaaaa aggcggtgtc agaatccagc catgagctgg cgcagttgag ttttctggtg
    10741 caggtcgaaa gggcttgcgt gcgtcacttt cccgctttgc gcggttaaag tgacgcacgg
    10801 atctggtgca gcatctgaga tttatcgtct ggtgcagttt tacggtcacg cggcagcaaa
    10861 gcctgacggc aatgcaggcc gcagcccgta aggtaaaagg cgctgagggt gtcagcagat
    10921 gcaggttagc cgggagaatt ccggcgcggt gcagattcag accagcaggc tggcgaagta
    10981 aaaggatgta tcgtattaaa actctggcct ccgtcgcgat gttctgcgac ccggtgtttg
    11041 cagaggtctg caccggtaaa cggatcgacc gtcctgataa caggcgcttc tttcaggttg
    11101 tgaagcgttg ggcgctgtct acgacagcat agagcaaggg ggagactacg cctgtgccgc
    11161 tggcgcggaa aggagaaact gtttttacct gcaaaaggat tttttaatca cttgttgtta
    11221 taaaaaatga gggcgctgta tgtgacagcg ccctcgttca ggttacataa aaaatatcag
    11281 ctcagaatag cttacagtcg agccagcacc tcattcacga cagcatcggg tatacgttcc
    11341 agccgcttac cgctccgagc tcccatatca atggttctgg gctggtcgca gcgaataacg
    11401 cccgttgttt ttgttcccgc cccgtccagt gaaacggtaa aaccggcttc gcgggcaaag
    11461 tttccgccgc tggtgacagg gacaacaaca ggcaaccggg tgaacgtgtt aaacgatgcc
    11521 ttcgatacga tgagtaccgg acgttttcca ctttgttcat ggccagtggt gggatccagt
    11581 gaaacgagcc agatttctcc tctgtccatc tacaggatct ccttgcccac cgcaggcgca
    11641 tcaatccatt cccgaccctc ttcgcttatt tcagcgtgcg gatcgcactg cgccaacagt
    11701 tcttcgagcg aatagcgggg acgtttttga ggttcaataa tcaggcagcc attatcaatg
    11761 gtcatgccca cttcgctgtc cgtcgacagc cctagcgttt tcagcactgc ggggggaacc
    11821 gccagcatga cggatccgcc aacctttttc aggcgagtgg tatacataga gcacctccgg
    11881 atattatatt ttaatataac atccatgata gacgatgcac aactattcat cagattcatt
    11941 gtggtttcct tagtgtaata aagcgtgaag aaagccttaa tccatcggaa gacagatccg
    12001 gcagttttca aaggcgacat tgccggtaaa agggcaaccg tcctgataca tcaggcgctt
    12061 ctttcaggtc atgaagcatt tcgtacaggc gggagcttag tgcgtaacaa agaaggccga
    12121 taataggaaa ctgcttttgc agttcaaagg attatgacgg ttcggataaa caaaatgcgg
    12181 cgcagcttcc gtatccgggc tgcgccgcag gaacatcagc ggtagcgcga aggccagatt
    12241 tgctcaggct tgatgcctaa cgcttcggca attaaccttt cgcctttggg ccagcggcgg
    12301 gtaagcgcat tagccagcgt ggaggacgcc agacctgcct gacgcgaaag cgctgacagc
    12361 gaagtgcctc gttttttcag cccggcgata atgtcggcag gatgccagtc ctgcagcatc
    12421 atgcagccac ctcgtcgata aacttcacgc catcggcggt gcggagccag cgcggggctt
    12481 tcatttcggc ggcaaaatac cagaccagtt cacataataa gcctgttatc gtctgctcca
    12541 cttttggctg catcccctgc ccgataagaa gttgcgccag cgtcagtgag taatcacaaa
    12601 gtaattctgc gtcggggaca aatcggggca tatcggcagg tagagcatca acggtcaggc
    12661 tgtcagtcag atgagacggg atggggtcgt tcagcgtggg ttgcagcaaa gtgagacagg
    12721 cggcaagtcg cccgcaaagc gccagccgca gcaccggatc ttcgctttcg atcagggttt
    12781 cggcgacgtt ttcgcagtgg tctgccagta cggtaaaatc tgtggcagca ctgaagggaa
    12841 cagcaagtaa cgggtgtgtc tgagtgggga tcgtagccat aaaggcagcc tccagtatgt
    12901 ggtttgaaaa ccaccaccag aggttccaat ctcatgggtg gtggactgaa cgaggttgga
    12961 actaccggcc atactggaac cggcgcacct tacggtgccc ccgcccagcc caccataatt
    13021 ttggtgtagc taagcgcgcg acataaaaaa agacgcagac gcgtcacgtg tcgccagtat
    13081 ggttatattt cggggttcca atcccggcac ctgattttgc aggtgcgggt aaaagatacc
    13141 gctttcggtt gaaaacggca aggggaagtc gtggaataga gatcgcagat tgttataaat
    13201 atacacaata gttaatgtta acaaagacgg cacatatatt aactttactc tcaatctgct
    13261 gaatagttta tcgcatgtaa ttctcgggtc atatagtgga taccctcctt cttttctttc
    13321 atcccctctt gcctcaccat aaaagcaaac gaaaaaattg ctgaacgagt tatctgaagt
    13381 aaatgctcaa aatggtgaat aaaatcactt tctttgataa aaagaatatc ttctattagt
    13441 tgataagatt cgtaaatgtc ctcaggttta tcacttttat gtacaactaa aaacttatgc
    13501 tctaaaccat tgcgccattc tttatagaaa gccaactccc cacccttatc tttattcagg
    13561 tcggtagcaa tactgtacag cgccagaagc cctggtgatt ttaccttttc aaataattgt
    13621 cttctgttat caacatctaa ctgccagaaa ttctgaaaat agatattctt ctgaggtgta
    13681 cctttttttg ttgttgggta aagattataa agttcacata tggctaccgc aattttatca
    13741 agaataccaa agcaaagacg aaatgccgtc ctgatttttt caatttctat tcctaaaact
    13801 tcatcattat acaattccag aaaatggctt tcgtacttaa tgtaatccgt attttgtgga
    13861 tgaagataat caaaataaag atggcgtgcg agtgaaaact ccgatttaag gcgatttaat
    13921 accatctcca tgggaatgat aaaatcacct gttacaccgc ctcgcgaagc aatcacaaga
    13981 tcatctgtag cactccctac acatgggcaa tacaggccat gttctgataa agtcagatga
    14041 tttctaagac aaaactgtcg atatgggctt aacacttcga actcttgttg tgtaagtgct
    14101 tcatcgtgtt cgtcagtttc atcatcaaca gcatattctg cgattttctc gttagtctgc
    14161 gctatgcgtt ctaaatagaa cgattcccat tgtggtggga tattttttga tacagacgct
    14221 ttaatatatc ctgctctcac ttcttttagt aacttgattg aaaatgaaga tgaaataaga
    14281 ttgaggacca taagtgacat cgaacggttg acccaagcct gtggtatatc taatccctta
    14341 agattggtaa gatcataata tctcaatgac tcactgagcc gaaattgact tcttaatgaa
    14401 ttagccaaat taacagatag ttcagcctga aattcctcct gctcctcagc agatagctta
    14461 aaagaacgcc agtagatatt ttttaaggaa accaattctt ctatcgattt gaatgtatgg
    14521 tcattggggt tagtaatgct caccagatta gatagcgcat taccatagtt ataatagtaa
    14581 aggcactctc ctaacactga tataaatgct tctttgttat cttccatcag gttgcaacca
    14641 agctctgacg catttttatt attttgcatc tgcccgatgt caacaagagc acccgcaatg
    14701 ttcgccaata ctataaagta gttatcgttc ctgattgtta ttctttccag tttgttcagg
    14761 aacgcaactg actgttgata agattcgtca aatcttccaa tatcaaccag ttggctaatg
    14821 aaattacttt catcgacaag ttcttttgtt atcaaaataa attttcctta tacagaggat
    14881 ttcggtgctt caacaggagg tatgccctca caggagacat ctataactat cgctgataag
    14941 cggtctcttt tatcaaccgg cacagtttca ttaagcatat tttccattgc actggcgttt
    15001 tcaggttgag atgtattttt cgccaaatga ggcagggaat tcggtcgagc agaaccaaac
    15061 caaaaaacca gatagatacc gtgaccagca gcatcggggt ttttagtata aagcttgtcc
    15121 aattgcccat taagtgcagt ccagacatca cgatgataat cacgctttat ctcgataggt
    15181 atttttatct gcggtagtga aacaatgata tctgcccttt tatcagaaac catgtgtcct
    15241 tctggttcac agcttatttt ttatggatta agccggacac ggagtaaatc cagaaaaata
    15301 ttacggcaac tctcttcagg tttaggcgtt agggtacggc catagctgtc ttcattccag
    15361 aattgcttat aaatatcggt attttcattg gcaattcggt tagaaatatc ccgtatatga
    15421 tcaagcaata atgagtaaag atccattatg ttggcaggcg ttttattcat caatgtactt
    15481 acagtctgcc tccagtcggc atgatggaac tgtgattctc tataacgaat tttctgattg
    15541 ctttgggcat gtgagagatg atcccgataa ctttcacatt caggcagcag taataagcgc
    15601 tcaagtactt c
//
LOCUS       DAJVOY010000089        15582 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVOY010000089 DAJVOY010000000
VERSION     DAJVOY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.089"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            285..377
                     /locus_tag="OQL90_002457"
                     /pseudo
     CDS             285..377
                     /locus_tag="OQL90_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            381..560
                     /locus_tag="OQL90_002458"
                     /pseudo
     CDS             381..560
                     /locus_tag="OQL90_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            894..3173
                     /gene="ydeP"
                     /locus_tag="OQL90_002459"
     CDS             894..3173
                     /gene="ydeP"
                     /locus_tag="OQL90_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1369199.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3421..3618
                     /gene="safA"
                     /locus_tag="OQL90_002460"
     CDS             3421..3618
                     /gene="safA"
                     /locus_tag="OQL90_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1369200.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3693..4454
                     /gene="ydeO"
                     /locus_tag="OQL90_002461"
     CDS             3693..4454
                     /gene="ydeO"
                     /locus_tag="OQL90_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1369201.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4856..6538
                     /locus_tag="OQL90_002462"
     CDS             4856..6538
                     /locus_tag="OQL90_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1369202.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6590..7747
                     /locus_tag="OQL90_002463"
     CDS             6590..7747
                     /locus_tag="OQL90_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1369203.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7713..7835)
                     /locus_tag="OQL90_002464"
     CDS             complement(7713..7835)
                     /locus_tag="OQL90_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369204.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8044..9729
                     /locus_tag="OQL90_002465"
     CDS             8044..9729
                     /locus_tag="OQL90_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCS1369205.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            9767..12139
                     /locus_tag="OQL90_002466"
     CDS             9767..12139
                     /locus_tag="OQL90_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1369206.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12196..14979
                     /locus_tag="OQL90_002467"
     CDS             12196..14979
                     /locus_tag="OQL90_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1369207.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15341..>15582
                     /locus_tag="OQL90_002468"
     CDS             15341..>15582
                     /locus_tag="OQL90_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1369208.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4540 a         3390 c         3408 g         4244 t
ORIGIN      
        1 gagaatacgg tagtaagtga gatatggggt ttggtcgttg ttaatgagca accatgacca
       61 tgaccgccaa cgactggatt ctcccttggt tatgttggtg cgcataatgt atattatgtt
      121 aaatcatatc tgagtgatgc gaagtgcttt tctggtagtc gttattcgtt cagggttcca
      181 gaacttaagc ctctcctctt tcataattgg cctgtgcatg acggatatag gctggctggt
      241 atgcagattt gccgtttgcc gcatcttccc agcgttgaag actcaagctg gcaaaccgcg
      301 tgcaaatgag cacgccctct ttccatcaac acttcaggca gcttgcggga atgagtccac
      361 tacggtatca gaaatgggaa gtgggtgtct ctctgatgcg taatggaaaa atactggcta
      421 cgggagaaaa tgtctccctg gggacagtaa ataaaagcaa agtatctctg ggtttaagcg
      481 caacctatgg acaaacagga aacaaggttg cagccggaac cgtccagtca gtcattggtg
      541 tcacttttat ctatgaataa ttgtgatttt gatcttagtc tatgcgctcc attcacgaaa
      601 attgcgcgga taattatttt gtgaaggcta ttatcctaca cctgtaagaa aatccgcgca
      661 caattgaagc cagagtctta ttttttatat tgaaaaatat gttaatttaa acactatgag
      721 agatgatgct taacttatcg tatttaatct attgtttaac gataatagat taaaaataga
      781 aagagagtag tcccctactc ttatatatcc atgttggcga taatcccttt gtatgtactg
      841 tgcatcatcg ctattacaaa tcctaataat tcatttccac acaggataag tagatgaaga
      901 aaaaaattga atcctaccag ggtgctgcag gtggttgggg tgctgttaaa tccgtagcga
      961 atgcagtacg taagcagatg gatatacgcc aggatgttat tgccatgttt gacatgaata
     1021 agccagaggg ctttgactgt ccgggttgtg catggccaga tcctaagcac actgcgtcat
     1081 tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa
     1141 atgcctcttt ctttgctgag aatacggttc aatcattact tacctgggga gatcacgagc
     1201 ttgaggctgc gggacgtctc actcagcctt tgaaatatga tgccgtcagc gactgttaca
     1261 agccattaag ctggcaacaa gctttcgacg aaattggcgc acgccttcaa agctatagtg
     1321 atcccaatca ggttgaattc tatacttcgg gccgcacttc caatgaagct gcctttcttt
     1381 atcagctttt tgcccgtgaa tacgggagca ataactttcc cgactgctcc aacatgtgcc
     1441 atgaaccgac cagtgtgggt ttggcagcga gtatcggcgt gggtaaaggg actgtgttgc
     1501 tggaagactt tgagaagtgc gatttagtca tttgcattgg gcataaccct ggtacaaacc
     1561 acccccgcat gctgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcca
     1621 tcaatccttt acaggaacgt ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa
     1681 tgctgacgaa ctctgagact cagttggcca gtgcgtacta caacgtgcgc attggtggcg
     1741 atatggcgtt gctcaagggg atgatgcgcc tgttaattga gcgcgatgat gctgcaagtg
     1801 ccgcaggtcg gccctcattg cttgatgacg aatttattca aacgcatacc gtcggctttg
     1861 acgagctacg ccgtgacgtt ctcaattccg agtggaaaga tatcgaacat atttctggac
     1921 taagtcagac acaaatcgcc gaactggctg acgcatatgc cgctgccgat cgcaccatta
     1981 tctgttacgg aatggggatc actcagcacg aacatggtac ccagaatgta cagcaactgg
     2041 tcaatctgct gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc tgcccactac
     2101 gaggtcactc taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgcag
     2161 agtttctggc tcgcctgggt gagcgctatg gcttcacccc acctcatgca cctggacatg
     2221 ctgcaattgc cagcatgcaa gcaatatgca cggggcaggc tcgagcattg atctgcatgg
     2281 ggggcaactt tgcactggca atgccagatc gggaagcgag cgctgtaccg ttaacgcaat
     2341 tagatttggc ggtacacgta gccactaagc ttaaccgctc tcatctgctg accgcacggc
     2401 atagctatat tctgccggtt ctggggcgta gcgagattga catgcaaaaa agcggtgcgc
     2461 aggcggtaac cgttgaggat tcaatgtcga tgattcatgc ctcgcgtggc gtgttaaaac
     2521 ccgccggtgt aatgctgaaa tcagagtgtg cagtggtcgc gggaatcgcg caggcagcac
     2581 taccccagag cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc attcgcaatg
     2641 acattgaagc tgtgttacca gagttcgccg actataacca gcgcatccga catcctggag
     2701 gttttcacct gataaatgca gctgctgaaa ggcgctggat gacgccgtca ggtaaggcta
     2761 atttcattac cagcaaaggg ctgttagaag atccctcttc agcgtttaac agtaagctgg
     2821 tcatggcgac agtacgcagc cacgatcagt acaacacgac gatttatggt atggatgatc
     2881 gctatcgagg ggtattcggt caacgagatg tggtctttat gagtgctaaa caagctaaaa
     2941 tttgccgtgt aaaaaacggc gaaagagtta atcttattgc gcttacgcca gacggtaagc
     3001 gcagctcacg ccgcatggat agattaaaag tggtcattta ccctatggct gaccgctcac
     3061 tggtgaccta ttttccagaa tcgaatcaca tgctaactct tgataaccac gatccattaa
     3121 gtggcattcc tggttataaa agtattccgg ttgaattaga accatcaaat taattcgcct
     3181 cctcatttct tctgctgtca tccgcacagc agaagaattt ctcattgact attatcgcgc
     3241 aatttgctca cattgatcaa attaaaccac acactataag atataaacct ctgcctacag
     3301 ctgtaagaaa ctccgctcag tactgaagtg ccagtcctat ttcctctttt ctccagcctg
     3361 ttatattaag catactgatt aacaattttt aacgttatcc gctaaataaa catatttgaa
     3421 atgcatgcga ccacagtgaa aaacaaaatc acgcaaagag acaactataa agaaatcatg
     3481 tctgtaattg tggttgtctt attactgacc cttacgttga tagccatttt ttcggcaatt
     3541 gatcagctgg gtatttcaga aatgggtcgc attgcaagag atcttacaca tttcattatc
     3601 aatagtttgc aagactgaaa ataaacagca aattataaat atgaaatgtt aaaaaagtat
     3661 cgataaaaac tttattgttt taaggagata aaatgtcgct cgtttgttct gttatattta
     3721 ttcatcatgc cttcaacgct aacattttag ataaagatta cgccttctct gacggcgaga
     3781 tcctgatggt agataacgct gttcgtacgc attttgaacc ttatgagcgg cattttaaag
     3841 agatcggatt tactgaaaat accattaaaa aatatctaca atgcactaac atccagacag
     3901 tgacggtgcc tgttcctgcg aagtttttac gtgcttcaaa tgtaccgact ggattgctta
     3961 atgaaatgat tgcttatctc aactcggaag aacgcaatca tcataatttt tcagaacttt
     4021 tgcttttttc ttgcctgtct atttttgccg catgcaaagg tttcattaca ctattaacta
     4081 acggtgtgct atccgtttcc ggaaaagtga gaaatattgt caacatgaag ctggcgcacc
     4141 catggaagct gaaagatatt tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa
     4201 aactaaagca agagcaaacg acattctcac agattctatt agatgccaga atgcagcacg
     4261 caaaaaattt gatacgcgta gaaggttcag tcaataaaat tgccgaacaa tgtggttatg
     4321 ccagtacttc ttattttatt tatgcgttcc gcaaacattt cggcaacagt ccgaagagag
     4381 tttctaagga gtaccgttgt caaagtcaca cgggtatgaa tacgggcaac acgatgagtg
     4441 ctttagctat ttgattattt gctaacgagt agtcaaccac gcacgctacg taagaattat
     4501 atggggcagc cattccctgc cccgcgttgt ttttaggcga tatatttatt gaaataaata
     4561 agtgacatcc atcacatatt tatgcacttg cataacctgt tgcatgatta tttatgttct
     4621 caattcagca tcttgtcagt aaaatgcaat aatttattaa atatcaataa attagttgtt
     4681 tatcggcgag aaattactta atagaacaga aagtaatgtc aacgctttat ggactgttat
     4741 ttcccttttt ttagctaaat ctgctatctc tttatgtgac taacttcact tacatccact
     4801 tatttctctt cgtaaaatta cttcggaatt aagtacaata agaagaggaa catttatgaa
     4861 gtctgcatta aagaaaagtg tcgtaagtac ctcgatatct ttgatactgg catctggtat
     4921 ggctgcattt gctgctcatg cggcagatga tgtaaagctg aaagcaacca aaacaaacgt
     4981 tgctttctca gactttacgc cgactgaata cagtaccaaa ggaaagccaa atattatcgt
     5041 actaaccatg gatgatcttg gttatggaca acttcctttt gataagggat cttttgaccc
     5101 aaaaacaatg gaaaatcgtg aagttgtcga tacctacaaa atagggatag ataaagccat
     5161 tgaagctgca caaaaatcaa cgccgacgct cctttcatta atggatgaag gcgtacgttt
     5221 tactaacggc tatgtggcac acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg
     5281 tcgagctccc gcccgctttg gtgtctattc caataccgat gctcaggatg gtattccgct
     5341 aacagaaact ttcttgcctg aattattcca gaatcatggt tattacactg cagcagtagg
     5401 taaatggcac ttgtcaaaaa tcagtaatgt gccggtaccg gaagataaac aaacgcgtga
     5461 ctatcatgac aacttcacca cattttctgc ggaagaatgg caacctcaaa accgtggctt
     5521 tgattacttt atgggattcc acgctgcagg aacggcatat tacaactccc cttcattgtt
     5581 caaaaatcgt gaacgtgtcc ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc
     5641 aattggcgtt gttgatcgtg ccaaaacact tgaccagcct tttatgcttt acctggctta
     5701 taatgctccg cacctgccaa atgataatcc tgcaccggat caatatcaga agcaatttaa
     5761 taccggtagt caaacagcag ataactacta cgcttccgtt tattctgttg atcagggtgt
     5821 aaaacgcatt ctcgaacaac tgaagaaaaa cggacagtat gataatacta ttattctctt
     5881 tacctccgat aatggtgcgg ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg
     5941 ctataagagt cagacctatc ctggcggtac tcacacccca atgtttatgt ggtggaaagg
     6001 aaaacttcaa cccggtaatt atgacaagct gatttccgca atggatttct acccgacagc
     6061 tcttgatgca gccgatatca gcattccaaa agaccttaag ctggatggcg tttccttgct
     6121 gccctggttg caagataaga aacaaggcga gccacataaa aatctgacct ggataacctc
     6181 ttattctcac tggtttgacg aggaaaatat tccattctgg gataattacc acaaatttgt
     6241 tcgccatcag tcagacgatt acccgcataa ccccaacact gaggacttaa gccaattctc
     6301 ttatacggtg agaaataacg attattcgct tgtctataca gtagaaaaca atcagttagg
     6361 tctctacaat ctgacggatc tacagcaaaa agataacctt gccgccgcca atccgcaggt
     6421 cgttaaagag atgcaaggcg tggtaagaga gtttatcgac agcagccagc caccgcttag
     6481 cgaggtaaat caggagaagt ttaacaatat caagaaagca ctaagcgaag cgaaataact
     6541 aaaccttcat gcggcggaat tttccgccgc cttattgagc gagatagcga tgcacgttac
     6601 agccaagccc tccagttttc aatgtaatct caaatgtgat tactgttttt accttgaaaa
     6661 agagtcgcag tttactcatg aaaaatggat ggatgacagc actctgaaag agttcatcaa
     6721 acaatatatc gcagcgtctg gcaatcaggt ctattttacc tggcaaggcg gtgaacccac
     6781 tctggctggc ctggattttt tccgtaaagt tattcactat caacaacgct atgcaggcca
     6841 aaaacgtatt tttaatgcat tacaaacgaa tggcatttta ttgaataatg aatggtgttc
     6901 ctttctcaaa gaacatgaat ttctggttgg tatctcgatc gatggccccc aggagttaca
     6961 tgaccgttac agacgcagta attcaggtaa cggtactttt gcaaaagtga tagcagccat
     7021 cgagcgtctg aaatcatatc aagttgagtt taatacgtta accgtcatta ataacgttaa
     7081 tgtccattac cctcttgagg tttatcattt tttaaaatct atcggcagta aacatatgca
     7141 atttatcgaa ttgctcgaaa ccgggacgcc gaatattgat ttcagtggtc atagtgagaa
     7201 cacattccgt atcattgatt tttctgtgcc tcccacggct tatggcaagt ttatgtcaac
     7261 catttttatg caatgggtta aaaacgatgt gggtgaaatt ttcattcgtc agtttgaaag
     7321 ctttgtcagc cgttttttgg ggaatgggca tacaagttgt attttccagg agtcctgcaa
     7381 ggataatctg gttgttgaaa gtaatggaga catttacgaa tgcgaccatt ttgtctatcc
     7441 acagtacaaa attggaaaca ttaataaatc tgaactcaaa acgatgaaca gtgtacaact
     7501 gacagcgcaa aaaaaacgga tttcagcgaa atgtcagcaa tgtgcatata aacctatctg
     7561 caatggcggt tgtcctaagc atcgtattac taaagtaaac aatgagactg tttcttattt
     7621 ttgcgaaggt tataaaatcc ttttttcaac catggtacct tatatgaacg ccatggttga
     7681 gttagctaag aacagagtac cgctttacca cattatggat gttgcaaaac aaatggagaa
     7741 taattaataa cgctctcttt taactccatt cttagcctcc ttcttatgac cttaacaagg
     7801 cacccgacat aaattcctgt cgggtgtttc gtcatcatca tcttcccttg cgaaaatcct
     7861 ggcttacgtg gtatgcgtca gggcattagc aatacccttg attatcaata gcataaccat
     7921 aaccataacc actgaaaatt aaaaatttct atatatttca tattgttaaa tgatacttaa
     7981 ataaatttca tcagtaaatc attgccatag ataatcattg gatggcacaa tcaattttcg
     8041 ctaatgataa ccattcccat tacgttttgt atgttgatag caaagtatct ctgtctgctg
     8101 aagccctttt ggctgcgtaa gaataataaa acttcagtgt tgttaatcat tattatcctc
     8161 gcaatgatcc ttggcgtggt taaaattcag gtctggttaa acgactggaa taatgatttt
     8221 ttcaatgcgc taagccagaa agaaaccgat aagctttggc agcttgttct ttggttccct
     8281 gccctgctgg ggatctttgt attaatatcc gtaaacaaaa catggttaat taaactactg
     8341 actattcgct ggcgcgaatg gttaacagat tactacctta atcgctggtt tgcagataaa
     8401 aactactact tcacgcagat ctacggcgag cataaaaata cagataaccc tgaccaacgt
     8461 atcgctgaag acattctctt actgatcagc aaaacgctca gtctttcatt tggcttcatc
     8521 cagtcactta gcatgctgat cacctttacc gttattcttt ggcaaagcgc gggtacgctc
     8581 tcattcactg ttggtggaac agaatggaat atccaggggt atatggtcta taccgtcgtg
     8641 ctcattgtga tcggtggaac tttatttacc cataaggttg gtaagcgtat tcgtccgctt
     8701 aatgtggaaa aacaacgtag cgaagcgaca tttcgaacta atcttgtgca gcataataag
     8761 caggcagaac tgattgcgct aagcaatgct gagtctctac aacgccagga gttgcgcgac
     8821 aattttcata ccattaaaga gaactggcat cgtttaatga atcgtcagcg gtggcttgat
     8881 tactggcaaa atatttattc gcgctcgctt agcgttcttc cctactttct gttattgccg
     8941 cagtttatta gcgggcagat caatctgggc ggactgatga aatcgcgcca ggcatttatg
     9001 ctagtatcga acaatttaag ctggtttatt tataaatatg acgaacttgc tgaactggct
     9061 gcggttatcg atcgcttgta tgagttccat caactcactg aacagcgccc tacgaataag
     9121 cctaaaaatt gccaacatgc ggtacaagtg gctaatgcga gtattcgtac gcctgataat
     9181 aagatcatat tagagaactt gaactttcat gtttcgccag gcaaatggct attactgaaa
     9241 ggctactctg gtgcgggaaa aaccacactg cttaaaacat tatcccactg ctggccgtgg
     9301 tttaaaggtg atattttttc tcctgctgac agttggtatg tgtcacaaac accgttaatc
     9361 aaaaccggct tactgaaaga gattatttgt aaagcacttc ccctgcccgt agacgataaa
     9421 tcgttgagcg aagtactgca tcaggttggt cttgggaaat tggctgcgcg tattcatgac
     9481 cacgatcgct ggggagatat tctttccagc ggcgaaaaac aacgtatcgc cctggcacga
     9541 ttaattttac gacgtccgaa atggatattt cttgacgaaa ctacctctca tcttgaggaa
     9601 caagaggcta tccgcttact gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt
     9661 atggttacac atcaacccgg tgtctggaac ctggccgatg atatttgtga cattagcgcg
     9721 gttttataaa aacaataaaa gccctgctgt acggagatat aaataaatga agcgagttct
     9781 tattcctggc gtcattttat gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat
     9841 gtacatgcat ttttttgaag agatgacggt ctatgctcct gtccctgtac ccgtaaacgg
     9901 caacacgcat tacaccagtg aaagcatcga gcgtttaccg accgggaatg gcaatatcag
     9961 cgatctgctg agaaccaacc ctgcggtacg catggattca acgcaaagta cctcgttgaa
    10021 ccagggagat attcgccctg agaaaatctc tattcacggt gcgtcgccct accagaatgc
    10081 ctatttgatt gacggtatta gtgcaactaa taacctgaac ccagcgaatg agtccgatgc
    10141 cagtagtgca accaatatta gcgggatgtc acaggggtat tatcttgatg tcagcttact
    10201 ggacaatgtg acgctttatg acagttttgt gccggttgaa tttggtcgct tcaatggcgg
    10261 ggtaattgat gcaaagatca aacgcttcaa cgctgatgat agcaaggtga aattgggtta
    10321 tcgcactacg cgttcggact ggttaacatc gcatatcgat gagaataaca agagcgcatt
    10381 taatcaaggt tcttcaggaa gtacttattt ctctccagat tttaaaaaga acttttatac
    10441 cttgtcgttt aatcaggagc tggctgataa ctttggcgtt accgccggtt tatcgcgccg
    10501 ccagtctgat atcacccgcg cggattatgt ttcgaatgac ggcattgtcg tcggtcgggc
    10561 acagtataaa aacgttatcg atactgcatt gagcaaattt acctggtttg ccagcgaccg
    10621 ctttacccac gatttaacct taaaatatac cggctccagc cgtgattata ataccagcac
    10681 cttcccgcag tctgatcgcg aaatgggtaa taaatcctat ggtcttgcat gggatatgga
    10741 tacgcaactc gcatgggcca aactacgtac caccgttggt tgggatcata ttagtgatta
    10801 tacccgtcac gatcatgaca tctggtacac cgaactttca tgtacatatg gtgatattac
    10861 agggcgttgt acccgtggcg gattaggaca catttcccag gctgtagata attacacctt
    10921 caaaacacgc ctggactggc aaaaattcgc cgtgggtgat gtttcgcatc aaccctactc
    10981 cggcgcggaa tacatctatt ccgatgcatg gactgaacgc cataaccagt ctgaatccta
    11041 tgtgattaat gctgcaggaa agaaaactaa ccataccatt taccataaag gtaaaggcag
    11101 cctaggaatt gacaactaca cgctgtatat ggcggatcac attagctggc ggaatgtgtc
    11161 gttaatgccc ggtgtgcgtt atgactatga caactatctg tcaaaccaca atatctcccc
    11221 gcgctttatg acggaatggg atatttttgc tgatcaaacc tcaatgatta ccgccggtta
    11281 taaccgttac tatggcggga atattcttga tatgggatta cgtgatatcc gcaatagctg
    11341 gacggaatcg gtatcaggta ataaaaccct gacgcgttat cagaatttga aaacgcctta
    11401 taacgatgaa ctggcaatgg gattgcagca aaaaatcggt aagaacgtta ttgcacgcgc
    11461 aaactatgtt taccgtgaag cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc
    11521 gactaaaacc accattactg aatataacaa cgatggcaaa accaaaacgc attcgtttaa
    11581 cctcagtttt gaactggccg aacccctgca tatccgccag gtagatatta acccgcaaat
    11641 tgtctttagc tatatcaaga gcaagggcaa cttgtcgtta aacaatggtt atgaggagag
    11701 caataccggt gataaccagg tggtttataa cggtaatctg gtctcttacg atagcgttcc
    11761 agtggcagat tttaataacc cattaaagat ctccttaaac atggatttca cgcatcaacc
    11821 gagcgggtta gtgtgggcga atacgctggc gtggcaagaa gcgcgtaaag ctcgcattat
    11881 ccttggtaag acaaatgcgc aatacatcag cgaatattca gattacaagc agtatgttga
    11941 cgaaaaactg gatagcagcc tgacctggga cacccgcttg tcctggacgc cacaatttct
    12001 gaaacaacaa aacctgacga tcagtgccga tattctcaat gtactggata gcaaaaccgc
    12061 tgttgataca acgaataccg gtgtggcgac ctacgccagt ggccgtactt tctggcttga
    12121 tgtcagcatg aaattttaat agttcggatt ttacttaacc gggtaaattc gcccggtttt
    12181 cgcatggaga taattatgag aaacctctgt ttcttactga cgttagtggc aactctgttg
    12241 ctccccgggc ggctgattgc cgccgcctta ccgcaggatg aaaagttaat taccgggcaa
    12301 ctggacaatg gcttgcgata tatgatttat ccgcatgctc aaccaaagga tcaggtaaat
    12361 ttatggctgc aaattcatac cggttcattg caggaagaag acaatgagcg cggcgtggct
    12421 cattttgtag aacatatgat gtttaacggc acaaaaacat ggccgggtaa taaagtcatc
    12481 gaaacatttg agtcaatggg cctgcgtttt ggtcgcgatg ttaatgccta taccagctat
    12541 gacgaaacgg tgtatcaggt gagtttgccg actacgcaga aacaaaatct gcaacaagtg
    12601 atggcaatct tcagtgaatg gagtaatgcc gcaacctttg aaaaactcga agtagacgct
    12661 gaacgtggcg taattactga ggaatggcgt gcccatcagg atgcgaaatg gcgcacctct
    12721 caggcgcgcc gccctttcct gctggctaat acccgtaatt tagaccgtga acctatcggc
    12781 ctgatggata ctgtcgccac ggtcacaccg gcacaattgc gccaatttta tcaacgctgg
    12841 tatcaaccaa ataatatgac ctttatcgtg gtcggcgata tcgacagtaa aaaagcgctg
    12901 gcgctgataa aggataattt aagtaagctt ccggctaaca aagcagctga aaatcgcgtc
    12961 tggccgacaa aagccgaaaa ccacctgcgc tttaatatca ttaatgataa agaaaaccgg
    13021 gtgaacggca tcgcactcta ttatcgcctg ccaatggtac aagtgaacga tgagcaaagc
    13081 tttatcgaac aagctgaatg gagcatgtta gttcagctgt tcaatcaacg tctgcaggaa
    13141 cgcatacagt cgggcgagtt gaagactatt tctggcggca ctgcgcgcag cgttaaaatt
    13201 gcacccgatt atcagtcgct gtttttccgt gtaaatgcac gagacgataa tatgcaggat
    13261 gctgcgaatg cattaatggc agagttggca accattgatc agcatggttt ttctgctgaa
    13321 gaactcgatg atgtcaaatc tacccgcctc acctggctga aaaatgcggt tgatcagcaa
    13381 gctgaacgtg atttacgtat gctgaccagt cgcctggcat ccagctcatt aaataatacg
    13441 ccgttcttgt cgccggaaga gacatatcaa ctttcgaaac gtctgtggca gcaaattacc
    13501 gtgcaaagtc tggcggaaaa atggcagcag ttaagaaaga accaggacgc attttgggag
    13561 caaatggtaa acaatgagct tgccgccaaa aaagcattgt ctcctgcggc tatcctggcg
    13621 ctggaaaaag agtacgccaa caaaaagctg gcggcttaca tcttcccagg cagaaattta
    13681 tcgttaacag tagacgctga cccacaggcg gaaattagca gcaaagaaac gctggctgag
    13741 aatctgacat cattaacact ttccaatggt gccagggtta ttctggcaaa atccgcgggt
    13801 gaagagcaaa agctacaaat tactgccgta tcgaataaag gcgatttaag tttccctgcg
    13861 cagcaaaaat cacttatcgc gctggcaaat aaagcagtaa gcggaagcgg cgttggtgag
    13921 ctctcctctt ccagcctgaa acgctggagt gcggaaaatt cggtaaccat gagcagtaaa
    13981 gtcagtggca tgaatacgtt gctctccgtt agcgcgcgga ctaataaccc tgaacctggt
    14041 tttcagttga ttaaccagcg aatcacccac agcacgatta acgacaatat ttgggcatcg
    14101 ctacaaaatg ctcaaattca ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc
    14161 gcccagcaga tgtatgagac gcgctatgct gatgaccgca cgaaattact gcaagaaaat
    14221 cagattgtac agtttactgc cgcagatgcg ctggctgccg atcgccagtt gttttcatct
    14281 ccagcggata tcacgtttgt cattgtcggt aatgtctcag aagacaaact cgtggcgtta
    14341 attacgcgtt acttaggatc aatcaaacac tctgattcgc cattagccgc aggtaaacca
    14401 ttaactcgcg cgacggacaa cgcatcggtt actgtaaaag aacaaaatga acctgtggca
    14461 caggtttcac agtggaagcg ttatgattcc cggacacctg ttaatctggc gacgcgtatg
    14521 gcgctcgatg cttttaacgt cgcactggca aaagatctac gtgttaatat tcgtgaacag
    14581 gcatctggag catacagcgt ttcttctcgc ctctcggttg atcctcaggc caaagatatc
    14641 agtcatttgc tggcttttac ttgtcaacca gaacgacatg atgaactgtt aacgttggcg
    14701 aatgaagtga tggttaagcg tctggctaaa gggatcagtg agcaagaact gaatgaatac
    14761 cagcaaaacg ttcagcgcag cctcgatatc caacagcgta gcgttcaaca attagcgaac
    14821 actattgtaa atagtcttat tcaatatgac gatcctgcag catggactga gcaggagcaa
    14881 ttgttgaaac aaatgacggt agagaatgtt aacactgccg ttaaacaata tctttctcat
    14941 ccggtaaata cttataccgg agtattattg ccaaaataat aacagccccg tcaacacatc
    15001 gttggcgggg attttagcaa tattcgctat ttttatgtaa taattttata aatgcgttca
    15061 aaataataat caagtactaa tagtgatatt ttaaggtctg atttttacgt gataattcag
    15121 gagacacaga atgcgcataa aaataacagc ataaaacacc ttaccaccac ccaagaattt
    15181 catattgtat tgtttttcaa tgaaaaaaca ttattcgcgt aatatctcac gataaataac
    15241 attaggattt tgttatttaa acacgagtcc tttgcacttg cttactttat cgataaatcc
    15301 tactttttta atgcgatcca atcattttaa ggagtttaaa atggataaga agcaagtaac
    15361 ggatttaagg tcggaactac tcgattcacg ttttggtgcg aagtctattt ccactatcgc
    15421 agaatcaaaa cgttttccgc tgcacgaaat gcgcgacgat gtcgcattcc agattatcaa
    15481 tgacgaatta tatcttgatg gcaacgctcg tcagaacctg gccactttct gccagacctg
    15541 ggacgacgaa aatgtccaca aattgatgga tttatccatt aa
//
LOCUS       DAJVOY010000090        15379 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVOY010000090 DAJVOY010000000
VERSION     DAJVOY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.090"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            125..745
                     /locus_tag="OQL90_002469"
     CDS             125..745
                     /locus_tag="OQL90_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCS1369209.1"
                     /translation="MKDQDVRFAVHHKLLKESHLDPDCLVVDEFSISLGASRADIAVI
                     NGVIHGYELKSEYDSLERLPLQIKHYSSVMDKVTLVVAEKHLEGALKLIPGWWGVKTV
                     SVGPKGAILIKHMRGEKLNRNHDTLMLAQLLWKDECIDVLERWGYSKGIKSKPRFELW
                     NIIAENIPIANLRLEVRTALKKRVGWKVKAWQAESAPTNKAVSRLT"
     gene            complement(687..1754)
                     /locus_tag="OQL90_002470"
     CDS             complement(687..1754)
                     /locus_tag="OQL90_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HCS1369210.1"
                     /translation="MSQHQYYPQLKWKPAEYESLMLLDQTTLSGFTPIITIPDIDWDY
                     ENECYKKSLSSYLSDFGINLAASWKANRPVLLDVKYLDKHGSSRHHPLDMCIQDARVN
                     GKEIIPVVSPAYSTNYIHAVQRNLINGLAISITPQTWHQFTSLVNHLNIHPSLIDVII
                     DFGDIQNATDSLKQQALSMVNTLSGQAPWRNLILSSTAYPASQAGIPQHQVHHIPRHE
                     YDLWMYVVQNFSNGRTPSFSDYPTASSTITSVDPRFMSQYVSVRYSNDTSWIFVKGTA
                     VKGNGWGQTKNLCTTLVSSPEYQVFGSKFSWGDDYIYQRSLGANKSGGSKEWRKVAHT
                     HHITLVVRQLYWLAQTQPAKP"
     gene            2167..2712
                     /locus_tag="OQL90_002471"
     CDS             2167..2712
                     /locus_tag="OQL90_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCS1369211.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2687..4611
                     /locus_tag="OQL90_002472"
                     /pseudo
     CDS             2687..4611
                     /locus_tag="OQL90_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            4608..4814
                     /locus_tag="OQL90_002473"
     CDS             4608..4814
                     /locus_tag="OQL90_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCS1369212.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            4811..6412
                     /locus_tag="OQL90_002474"
     CDS             4811..6412
                     /locus_tag="OQL90_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1369213.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6393..7712
                     /locus_tag="OQL90_002475"
     CDS             6393..7712
                     /locus_tag="OQL90_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS1369214.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            7722..8054
                     /locus_tag="OQL90_002476"
     CDS             7722..8054
                     /locus_tag="OQL90_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1369215.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDSAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8110..9135
                     /locus_tag="OQL90_002477"
     CDS             8110..9135
                     /locus_tag="OQL90_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1369216.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGSTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            9177..9572
                     /locus_tag="OQL90_002478"
     CDS             9177..9572
                     /locus_tag="OQL90_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1369217.1"
                     /translation="MTKDELIVRLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTPLRPENALTGHENEVVSAQTDTVTDAAALVTVVALVTLHTDALHATRDEP
                     VAFVLPETAFRVSAGVAAEMTERGLARMQ"
     gene            9584..9937
                     /locus_tag="OQL90_002479"
     CDS             9584..9937
                     /locus_tag="OQL90_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCS1369218.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9949..10527
                     /locus_tag="OQL90_002480"
     CDS             9949..10527
                     /locus_tag="OQL90_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1369219.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            10524..10919
                     /locus_tag="OQL90_002481"
     CDS             10524..10919
                     /locus_tag="OQL90_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1369220.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            10927..11667
                     /locus_tag="OQL90_002482"
     CDS             10927..11667
                     /locus_tag="OQL90_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1369221.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDKDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            11683..12105
                     /locus_tag="OQL90_002483"
     CDS             11683..12105
                     /locus_tag="OQL90_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1369222.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            12087..12521
                     /locus_tag="OQL90_002484"
     CDS             12087..12521
                     /locus_tag="OQL90_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS1369223.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSIHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            12514..15075
                     /locus_tag="OQL90_002485"
     CDS             12514..15075
                     /locus_tag="OQL90_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1369224.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKVGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SQLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQCRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            15072..>15379
                     /locus_tag="OQL90_002486"
     CDS             15072..>15379
                     /locus_tag="OQL90_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1369225.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     "
BASE COUNT         3663 a         3780 c         4646 g         3290 t
ORIGIN      
        1 tagtcgtatg gctatgaatc ttgttgctgc agataagcat tttgtgattg aagtggtcaa
       61 atctctcaaa tgcagtaaaa tgcagtgcgc tataattcaa taacggaggg agtaaggaga
      121 agtcatgaaa gatcaagatg ttaggttcgc ggtgcatcat aagcttttga aagaatcgca
      181 tttagatcca gactgccttg tggtcgatga attttccata tcccttggcg ccagtagagc
      241 agacattgct gtaataaatg gtgttataca cgggtacgag ctcaaaagtg aatatgactc
      301 tttggagcgt ttgcctcttc aaatcaagca ttattcttct gtaatggaca aggttactct
      361 tgtcgtagct gagaaacatc ttgagggagc attaaagtta atcccaggtt ggtggggcgt
      421 taaaacggtt tctgttgggc caaaaggcgc cattcttata aagcacatgc gtggagaaaa
      481 gcttaatcga aaccatgaca cattgatgct cgctcaattg ctttggaaag atgaatgtat
      541 cgacgtactt gaacgatggg gctattccaa aggaatcaaa agcaagcccc gatttgagtt
      601 atggaatatt attgcggaaa atattccaat agcgaatctc aggcttgaag tcagaacagc
      661 cttaaagaaa cgcgtaggct ggaaagttaa ggcttggcag gctgagtctg cgccaaccaa
      721 taaagctgtc tcacgactaa cgtaatatgg tgcgtatgtg caactttacg ccattcttta
      781 gagccgccag atttgttagc gcctaatgat ctttggtaaa tgtaatcatc cccccaacta
      841 aatttggaac caaagacttg atactctggc gaactaacaa gggtagtaca taagttttta
      901 gtttggcccc atccatttcc tttaactgcg gtacctttta caaagatcca tgaggtatcg
      961 ttcgaatatc tcactgagac atactgagac atgaagcgtg ggtctacgct cgtaatggta
     1021 gagctagcgg tgggataatc actaaaactt ggcgttcttc cattgctaaa attctgtact
     1081 acgtacatcc aaagatcgta ttcatggcgc ggaatatgat gaacttgatg ttgcggtatc
     1141 cctgcctgtg atgctgggta tgcggttgaa gataaaatca agtttctcca cggagcttgg
     1201 cctgataatg tgttgaccat gcttaatgct tgttgtttta aactatcagt tgcgttttga
     1261 atatctccaa aatcaatgat tacatcaatt aaactaggat gaatatttaa gtggttaacc
     1321 agacttgtga attggtgcca tgtctggggg gtgatagata tagcgagccc attgattaag
     1381 ttgcgttgaa cagcatgtat atagtttgtt gaatatgcgg gagaaacaac agggataatt
     1441 tccttaccat ttactctagc atcttggata cacatatcta gaggatgatg gcggctcgaa
     1501 ccatgtttat ctaaatattt aacatccagc aaaacaggac gattggcttt ccaggatgcc
     1561 gcaaggttaa taccgaagtc agataagtaa gaactcaaac tcttcttgta gcattcgttt
     1621 tcataatccc agtctatgtc tggaatggta atgatcggag taaaaccaga gagcgtagtt
     1681 tgatctaaaa gcatcagaga ttcatattca gcaggcttcc atttcagctg tggataatat
     1741 tgatgttgac tcattaaaac tccttaaaat ctttacttat cctaagtata gtgttatggc
     1801 gtaacgccaa ttgtttttat acacttgaaa acattgggaa aagttgagct atatctaaat
     1861 ttacaaaatt ggatgttata gaaatcgagg tttcaactag gcatggaact gcaaaatttt
     1921 taatgcctgc acgcgaaagt cgttggcggg tcctttccgg tgatccagac cgttacgggg
     1981 cggcgacctc gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc
     2041 ttcttcgtcg taacttaatg tttttattta aaataccccc tgaaaagaaa ggaaacgaca
     2101 ggtgctgaaa acgaactttt gggcctttgt cgtttccttt ctctgttttt ggccgtggaa
     2161 tgaacaatgg aagtcaacaa aaagcagctg gctgacattt tcggtgcgag tatccgtacc
     2221 attcagaact ggcaggaaca gggaatgccc gttctgcgag gcggtggcaa gggtaatgag
     2281 gtgctttatg actctgccgc cgttataaaa tggtatgccg aaagggatgc tgaaattgag
     2341 aacgaaaagc tgcgccggga ggttgaagaa ctgcgccagg ccagcgaggc agatctccag
     2401 ccagggacta ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggaa
     2461 ctgaagaatg ccagagactc agctgaagtg gtggaaaccg cattctgtac tttcgtgctg
     2521 tcgcggatcg caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg
     2581 cgttttccgg aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc
     2641 atgaacaaag cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa
     2701 cagtcaggtt aacaggctgc ggcattttgt ccgcgccggg cttcgctcac tgttcaggcc
     2761 ggagccacag accgccgttg aatgggcgga tgctaattac tatctcccga aagaatccgc
     2821 ataccaggaa gggcgctggg aaacactgcc ctttcagcgg gccatcatga atgcgatggg
     2881 cagcgactac atccgtgagg tgaatgtggt gaagtctgcc cgtgtcggtt attccaaaat
     2941 gctgctgggt gtttatgcct actttataga gcataagcag cgcaacaccc ttatctggtt
     3001 gccgacggat ggtgatgccg agaactttat gaaaacccac gttgagccga ctattcgtga
     3061 tattccgtcg ctgctggcgc tggccccgtg gtatggcaaa aagcaccggg ataacacgct
     3121 caccatgaag cgtttcacta atgggcgtgg cttctggtgc ctgggcggta aagcggcaaa
     3181 aaactaccgt gaaaagtcgg tggatgtggc gggttatgat gaacttgctg cctttgatga
     3241 ggatattgaa caggaaggct ctccgacgtt ccttggcgac aaacgtattg aaggctcggt
     3301 ctggccaaag tccatccgtg gctccacgcc caaagtgaga ggcacctgcc agattgagcg
     3361 tgcagccagt gaatccccgc attttatgcg ttttcatgtt gcctgcccgc actgcgggga
     3421 ggagcagtat cttaaatttg gcgacaaaga gacgccgttt ggcctcaaat ggacgccgga
     3481 tgacccctcc agcgtgtttt atctctgcga gcataatgcc tgcgtcatcc gccagcagga
     3541 gctggacttt actgatgccc gttatatctg cgaaaagacc gggatctgga cccgtgatgg
     3601 cattctctgg ttttcgtcat ccggtgaaga gattgagcca cctgacagtg tgacctttca
     3661 catctggaca gcgtacagcc cgttcaccac ctgggtgcag attgtcaaag actggatgaa
     3721 aacgaaaggg gatacgggaa aacgtaaaac cttcgtaaac accacgctcg gtgagacgtg
     3781 ggaggcgaaa attggcgaac gtccggatgc tgaagtgatg gcagagcgga aagagcatta
     3841 ttcagcgccc gttcctgacc gtgtggctta cctgaccgcc ggtatcgact cccagctgga
     3901 ccgctacgaa atgcgcgtat ggggatgggg gccgggtgag gaaagctggc tgattgaccg
     3961 gcagattatt atgggccgcc acgacgatga acagacgctg ctgcgtgtgg atgaggccat
     4021 caataaaacc tatacccgcc ggaatggtgc agaaatgtcg atatcccgta tctgctggga
     4081 tactggcggg attgacccga ccattgtgta tgaacgctcg aaaaaacatg ggctgttccg
     4141 ggtgatcccc attaaagggg catccgtcta cggaaagccg gtggccagca tgccacgtaa
     4201 gcgaaacaaa aacggggttt accttaccga aatcggtacg gataccgcga aagagcagat
     4261 ttataaccgc ttcacactga cgccggaagg ggatgaaccg cttcccggtg ccgttcactt
     4321 cccgaataac ccggatattt ttgatctgac cgaagcgcag cagctgactg ctgaagagca
     4381 ggtcgaaaaa tgggtggatg gcaggaaaaa aatactgtgg acagcaaaaa gcgacgcaat
     4441 gaggcgctcg actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg
     4501 cagctggatc tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac
     4561 aagaaaacac tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg
     4621 aagaacttgc cgctgcccgt gcggcactgc atgacctgat gacaggaaaa cgggtggcaa
     4681 cggtacagaa agacggacgg agagtggagt ttacggccac ttccgtgtct gacctgaaaa
     4741 aatacattgc ggagctggaa gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag
     4801 gattttatgt atgaaaacgt ccaccattcc cacccttctg gggccggacg gcatgacatc
     4861 gctgcgtgaa tatgccggtt atcacggcgg tggcagcgga tttggagggc agttgcgggc
     4921 gtggaaccca ccgagtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa
     4981 tgcccgcgca gacgatctgg tacgcaataa cggctatgcc gccaacgcca tccagttgca
     5041 tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct
     5101 gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga
     5161 gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat
     5221 gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg
     5281 ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat
     5341 cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga
     5401 cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca
     5461 gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt
     5521 tgaacccgtg gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca
     5581 gatgaagatg ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat
     5641 gtatgccgcc accattgaga gtgagctgga tacgcagtca gcgatggatt ttattctggg
     5701 cgcgaacagt caggagcagc gggaaaggct gaccggctgg attggtgaaa ttgccgcgta
     5761 ttacgccgca gcgccggtcc ggctgggagg cgcaaaagta ccgcacctga tgccgggtga
     5821 ctcactgaac ctgcagacgg ctcaggatac ggataacggc tactccgtgt ttgagcagtc
     5881 actgctgcgg tatatcgctg ccgggctggg tgtctcgtat gagcagcttt cccggaatta
     5941 cgcccagatg agttactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat
     6001 ggggcggcga aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga
     6061 agaggccatc gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gttttcagga
     6121 agcccgcagt gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg
     6181 tctgaaagaa gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa
     6241 agagtgcgcg aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac
     6301 gatggagcgc cgtgcagccg gtcttaaacc gcccgcctgg gcggctgcag catttgaatc
     6361 cgggctgcga caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc
     6421 atattgccag catggccttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt
     6481 tcttttgtgc gcttgcaggc cagcttggga tcagccgcct gacggatgcg gtgtccggcg
     6541 acagcctgac tgcccaggag gcactcgcga cgctggcatt atccggtgat gatgacggac
     6601 cacgacaggc ccgcagttat caggtcatga acggcatcgc cgtgctgccg gtgtccggca
     6661 cgctggtcag ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca
     6721 ttatcgcccg tctgcaacag gctgccagcg atccgatggt ggacggcatt ctgctcgata
     6781 tggacacgcc cggcgggatg gtggcggggg catttgactg cgctgacatc atcgcccgtg
     6841 tgcgtgacat aaaaccggta tgggcgcttg ccaacgacat gaactgcagt gcaggtcagt
     6901 tgcttgccag tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg
     6961 gcgtcatgat ggctcacagt aattacggtg ctgcgctgga gaaacagggt gtggaaatca
     7021 cgctgattta cagcggcagc cataaggtgg atggcaaccc ctacagccat cttccggatg
     7081 acgtccggga gacactgcag tcccggatgg acgcaacccg ccagatgttt gcgcagaagg
     7141 tgtcggcata taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca
     7201 gcggtcagga ggccattgat gccggactgg ctgatgaact tgttaacagc accgatgcga
     7261 tcaccgtcat gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga
     7321 ccaaagagac tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactgacg
     7381 tggtgccagc gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga
     7441 tcaccgcagc ggttgcggca gaaaacagcc gcattatggg gatcctcaac tgtgaggagg
     7501 ctcacggacg cgaagaacag gcacgcgtgc tggcagaaac ccccggtatg accgtgaaaa
     7561 cggcccgccg cattctggcc gcagcaccac agagtgcaca ggcgcgcagt gacactgcgc
     7621 tggatcgtct gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg
     7681 ccgttaacga tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct
     7741 ttacccatta ccagccgcag ggcaacagtg acccggctca taccgcaacc gcgcccggcg
     7801 gattgagtgc gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc agccgtaagc
     7861 tggttgcgtg ggatggcacc accgacagtg ctgccgttgg cattcttgcg gttgctgctg
     7921 accagaccag caccacgctg acgttctaca agtccggcac gttccgttat gaggatgtgc
     7981 tctggccgga ggctgccagc gacgagacga aaaaacggac cgcgtttgcc ggaacggcaa
     8041 tcagcatcgt ttaactttac ccttcatcac taaaggccgc ctgtgcggct ttttttacgg
     8101 gattttttta tgtcgatgta cacaaccgcc caactgctgg cggcaaatga gcagaaattt
     8161 aagtttgatc cgctgtttct gcgtctcttt ttccgtgaga gctatccctt caccacggag
     8221 aaagtctatc tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt
     8281 gtttccggtg aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat
     8341 gtcaagccga agcatgaagt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat
     8401 ccgcagaatc tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt
     8461 gacgaagagc tggccattgc tcaggtcgaa gagatgcagg ccgtttctgc cgtgctcaag
     8521 ggcaaataca ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt
     8581 gcggcgaaca acatcacgca gtccggcagc acggagtgga gcaagcgtga caagtccacg
     8641 tatgacccga ccgacgatat cgaagcctac gcgctgaacg ccagcggcgt ggtgaatatc
     8701 atcgtgtttg atccgaaagg ctgggcgctg ttccgttcct tcaaagccgt cagggagaag
     8761 ctggataccc gtcgcggctc tcattccgag ctggagacag cggtgaaaga cctgggtaaa
     8821 gcggtgtcct ataaggggat gtatggcgat gtggccatcg tcgtgtattc cggacagtac
     8881 gtggaaaacg gcgtcaaaaa gaacttcctg ccggacaaca cgatggtact ggggaacact
     8941 caggcacgcg gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc
     9001 attaacgcct ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag
     9061 ttcaccatga ttcagtcagc accgctgatg ctgctggctg accctgatga gttcgtgtcc
     9121 gtacaactgg cgtaatcgtg gcccttcggg gccattttct ctctgtggag gagtccatga
     9181 cgaaagatga actgattgtc cgtctccggt cgctgggtga gcaactgaac cgtgatgtca
     9241 gcctgacggg gacgaaagaa gaactggcgc tccgtgtggc agagctggaa gaggagcttg
     9301 atgacacgga tgacgctgcc ggtcaggata cccctctcag accggaaaat gcgctgaccg
     9361 gacatgaaaa tgaggtggta tcagcacaga cggataccgt gactgatgcg gctgctctgg
     9421 tcacggttgt ggcactggtg acgctgcata ccgatgcact tcacgccacg cgggatgaac
     9481 ctgtggcatt tgtgctgccg gaaacggcgt tccgtgtctc tgccggtgtg gcagctgaaa
     9541 tgacagagcg cggcctggcc agaatgcaat aacgggaggc gctgtggctg atttcgataa
     9601 cctgttcgat gctgccattg cccgcgccga tgaaacgata cgcgggtaca tgggaacgtc
     9661 agccaccatg acatccggtg agcagtccgg cgcagtaata cgtggtgttt ttgatgaccc
     9721 tgaaaatatc agctatgccg gacagggcgt acgcgttgaa ggctccagcc cgtccctgtt
     9781 tgtccggact gatgaggtgc ggcagctgcg gcgcggcgac acgctgacca tcggtgagga
     9841 aaacttctgg atagaccgga tttcgccgga tgatggcgga agctgtcatc tctggcttgg
     9901 gcggggcgta ccgcctgccg ttaaccgtcg ccgctgaaag ggggatgtat ggccataaaa
     9961 ggtcttgagc aggccgttga aaacctcagc cgtatcagta aaacggcggt gcccggtgcc
    10021 gccgcaatgg ccattaaccg cgttgcttca tccgcgatat cgcagtcggc gtcacaggtt
    10081 gctcgtgaga caaaggtacg ccggaaactg gtaaaggaaa gggccaggct gaaaagggcc
    10141 acggtcaaaa atccgcaggc cagaatcaaa gttaaccggg gggatttgcc cgtaatcaag
    10201 ctgggtaatg cgcgggttgt cctttcgcgc cgcaggcgtc gtaaaaaggg gcagcgttca
    10261 tccctgaaag gtggcggcag cgtgcttgtg gtgggaaacc gtcgtattcc cggcgcgttt
    10321 attcagcaac tgaaaaatgg ccgctggcat gtcatgcagc gtgtggccgg gaaaaaccgt
    10381 taccccattg atgtggtgaa aatcccgatg gcggtgccgc tgaccacggc gtttaaacag
    10441 aatattgaac ggatacggcg tgaacgtctt ccgaaagagc tgggctatgc gctgcagcat
    10501 caactgagaa tggtaataaa gcgatgaaac atactgaact ccgtgcagcc gtactggatg
    10561 cactggagaa gcatgacacc ggggcgacgc tttttgatgg tcgccccgct gtttttgatg
    10621 aggaagattt tccggcaatt gccgtttatc tcaccggcgc tgaatacacg ggcgaagagc
    10681 tggacagcga tacctggcag gcggagctgc atattgaagt tttcctgcct gctcaggtgc
    10741 cggattcaga gctggatgcg tggatggagt cccggattta tccggtgatg agcgatatcc
    10801 cggcactgtc agatttgatc accagtatgg tggccagtgg ctatgactac cggcgcgacg
    10861 atgatgcggg cctgtggagt tcagccgatc tgacttatgt cattacctat gaaatgtgag
    10921 gacgctatgc ctgtaccaaa tcctacaatg ccggtgaaag gtgccgggac caccctgtgg
    10981 gtttataagg ggagcggtga cccttatgcg aacccgcttt cagacgttga ctggtcgcgt
    11041 ctggcaaaag ttaaagacct gacgcccggc gaactgaccg ctgagtccta tgacgacagc
    11101 tatctcgatg ataaagatgc agactggact gcgaccgggc aggggcagaa atctgccgga
    11161 gataccagct tcacgctggc gtggatgccc ggagagcagg ggcagcaggc gctgctggcg
    11221 tggtttaatg aaggtgatac ccgtgcctat aaaatccgct tcccgaacgg cacggtcgat
    11281 gtgttccgtg gctgggtcag cagtatcggt aaggcggtga cggcgaagga agtgattacc
    11341 cgcacggtga aggtcaccaa tgtgggacgt ccgtcgatgg cagaagatcg cagcacggta
    11401 acagcggcaa ccggcatgac cgtgacgcct gccagcactt cggtggtgaa agggcagagc
    11461 accacgctga ccgtggcatt ccagccggaa ggcgcaaccg acaagagctt ccgtgcggtg
    11521 tctgcggata aaacaaaagc caccgtgtcg gtcagtggta tgaccatcac cgtgaaaggt
    11581 gttgctgcag gcaaggtcaa cattccggtt gtatccggta atggtgaact tgctgtggtt
    11641 gcagaaatca ccgtcaccga cagttaatcc ggagagtcag cgatgttcct gaaaaccgaa
    11701 tcatttgaat ataacggtgt gagcgtcacg ctttctgaac tgtcagccct gcagcgaatt
    11761 gagcatctcg ccctgctgaa acgacaggca gaacaggcgg gatccagtct caatcgacag
    11821 gtgagcgtgg aagatctcgt cagaaccggt gcttttctgg tggcgatgtc cctgtggcat
    11881 agccatccgc agaagacaaa gatgccgtcc atgaatgaag ccgttaaaca aattgagcag
    11941 gaagtgctta ccacctggcc cacagaggca attgctcagg ctgaaaatgt ggtaatgcgt
    12001 ctgtccggta tgtctgagtt tgttgtgaat gatgcacctg aacaggcaga tgacgccggg
    12061 ccagcagagc ctgtttctgc gggaaagtgt tcgacggtga gctgagtttt gccctgaaac
    12121 tggcgcgtga gatggggcga cccgactggc gcgccatgct tgccgggatg tcatccacgg
    12181 agtatgccga ctggcaccgc ttttacagta tccattattt tcatgatgtt ctgctggata
    12241 tgcacttttc cgggctgacg tacaccgtac tcagcctgtt tttcagcgat ccggatatgc
    12301 atccgctgga tttcagtctg ctgaaccggc gcgaggctga cgaagagcct gaagatgatg
    12361 tgctgatgca gaaagcggca gggcttgccg gaggcgtccg ctttggcccg gacgggaatg
    12421 aagttatccc cgcttccccg gatgtggcgg acatgacgga ggatgacgta atgctgatga
    12481 cagtatcaga agggatcgca ggaggagtcc ggtatggctg aaccggtagg cgatctggtc
    12541 gttgatttga gtctggatgc ggccagattt gacgagcaga tggccagagt caggcgtcat
    12601 ttttccggta cggaaagtga tgcgaaaaaa acagcggcag tcgttgaaca gtcgatgaac
    12661 cggcaggcgc tggctgcaca gaaagtggga atttccgtcg ggcagtataa agccgccatg
    12721 cgtatgctgc ctgcgcagtt cactgacgtg gccacgcagc ttgcaggcgg gcaaagtccg
    12781 tggctgatcc tgctgcaaca ggggggtcag gtgaaggact ccttcggcgg gatgatcccc
    12841 atgttccggg ggcttgccgg tgcgatcacc ctgccgatgg tgggggccac ctcgctggcg
    12901 gtggcgaccg gagcgctggc gtatgcctgg tatcagggca actcaaccct gtccgatttc
    12961 aacaaaacgc tggtcctttc cggcaatcag gcgggactga cggcagatcg tatgctggtc
    13021 ctgtccagag ccgggcaggc ggcagggctg acgtttaacc agaccagcga gtcactcagc
    13081 gcactggtta aggcgggggt aagcggtgag gctcagattg cgtccatcag ccagagtgtg
    13141 gcgcgtttct cctctgcatc cggcgtggag gtggacaagg tcgctgaagc cttcgggaag
    13201 ctgaccacag acccgacgtc ggggctgacg gcgatggcac gccagttcca taacgtgacg
    13261 gcggagcaga ttgcgtatgt tgctcagttg cagcgttccg gcgatgaagc cggggcattg
    13321 caggcggcga acgaggccgc aacgaaaggg tttgatgacc agacccgacg cctgaaagag
    13381 aacatgggca cgctggaaac ctgggcagac aggacagcac gggcattcaa atccatgtgg
    13441 gatgcggtgc tggatattgg tcgtcctgat accgctcagg agatgctgat taaggcagag
    13501 gctgcgttta agaaagcaga cgacatctgg aatctgcgca aggatgatta ttttgttaac
    13561 gatgaagcgc gggcgcgtta ctgggatgat cgtgaaaagg cccgtcttgc gcttgaagcc
    13621 gcccgaaaga aggctgagca gcagactcaa caggacaaaa atgcgcagca gcagagcgat
    13681 actgaagcgt cacagctgaa atataccgaa gaggcgcaga aggcttacga acggctgcag
    13741 acgccgctgg agaaatatac cgcccgtcag gaagaactga acaaggcact gaaagacggg
    13801 aaaatcctgc aggcggatta caacacgctg atggcggcgg cgaaaaagga ttatgaagcg
    13861 acgctgaaaa agccgaaaca gtccggcgtg aaggtgtctg cgggcgatcg tcaggaagac
    13921 agtgctcatg ctgccctgct gacgcttcag gcagaactcc ggacgctgga gaagcatgcc
    13981 ggagcaaatg agaaaatcag ccagcagcgc cgggatttgt ggaaggcgga gagtcagttc
    14041 gcggtactgg aggaggcggc gcaatgtcgc cagctgtctg cacaggagaa atccctgctg
    14101 gcgcataaag atgagacgct ggagtacaaa cgccagctgg ctgcacttgg cgacaaggtt
    14161 acgtatcagg agcgcctgaa cgcgctggcg cagcaggcgg ataaattcgc acagcagcaa
    14221 cgggcaaaac gggccgccat tgatgctaaa agccgggggc tgactgaccg gcaggcagaa
    14281 cgggaagcca cggaacagcg cctgaaggaa cagtatggcg ataatccgct ggcgctgaat
    14341 aacgtcatgt cagagcagaa aaagacttgg gcggctgaag accagcttcg cgggaactgg
    14401 atggcaggcc tgaagtccgg ctggagtgag tgggaagaga gcgccacgga cagtatgtcg
    14461 caggtaaaaa gtgcagccac gcagaccttt gatggtattg cacagaatat ggcggcgatg
    14521 ctgaccggca gtgagcagaa ctggcgcagc ttcacccgtt ccgtgctgtc catgatgaca
    14581 gaaattctgc ttaagcaggc aatggtgggg attgtcggga gtatcggcag cgccattggc
    14641 ggggctgttg gtggcggcgc atccgcgtca ggcggtacag ccattcaggc cgctgcggcg
    14701 aaattccatt ttgcaaccgg aggatttacg ggaaccggcg gcaaatatga gccagcgggg
    14761 attgttcacc gtggtgagtt tgtcttcacg aaggaggcaa ccagccggat tggcgtgggg
    14821 aatctttacc ggctgatgcg cggctatgcc accggcggtt atgtcggtac accgggcagc
    14881 atggcagaca gccggtcgca ggcgtccggg acgtttgagc agaataacca tgtggtgatt
    14941 aacaacgacg gcacgaacgg gcagataggt ccggctgctc tgaaggcggt gtatgacatg
    15001 gcccgcaagg gtgcccgtga tgaaattcag acacagatgc gtgatggtgg cctgttctcc
    15061 ggaggtggac gatgaagacc ttccgctgga aagtgaaacc cggtatggat gtggcttcgg
    15121 tcccttctgt aagaaaggtg cgctttggtg atggctattc tcagcgagcg cctgccgggc
    15181 tgaatgccaa cctgaaaacg tacagcgtga cgctttctgt cccccgtgag gaggccacgg
    15241 tactggagtc gtttctggaa gagcacgggg gctggaaatc ctttctgtgg acgccgcctt
    15301 atgagtggcg gcagataaag gtgacctgcg caaaatggtc gtcgcgggtc agtatgttgc
    15361 gtgttgagtt cagcgcaga
//
LOCUS       DAJVOY010000091        15368 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVOY010000091 DAJVOY010000000
VERSION     DAJVOY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.091"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..592
                     /locus_tag="OQL90_002487"
                     /pseudo
     CDS             1..592
                     /locus_tag="OQL90_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490532.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            806..1954
                     /locus_tag="OQL90_002488"
     CDS             806..1954
                     /locus_tag="OQL90_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1369226.1"
                     /translation="MFEIDSLLLITSVIILSLLAVKLFDEISPIQLVDHGRNNQIDGM
                     RGFLAIFVLIHHAAIWHGYLLTGVWETPSSNLLTNLGQVGVSFFFMITGYLFFSKIRS
                     SDQDWVRLYISRFLRLTPMFIVSLCLVLLVIGFKSRWSVHVSPASLTVSLMRWAPFTA
                     LGMPNINGVKDSFTINAAVTWTLVYEWFFYFSLPVIAALFKRRVSIHMIIISVLVLVI
                     FLCYPSKKIHVISFLFGLLASYSNNYVHRVAKAKITPIMIIALLSYEMIYFPTTYAPL
                     PLIICGVCFILIASGCDFYGILRLNLTRKLGETTYSIYLLHGVFLYCVMTWVIPSNYS
                     NYFFIMLISITAFCVTLLSCITFKLIELPFINITKQTAMKIRGIIKNT"
     gene            complement(2234..2845)
                     /locus_tag="OQL90_002489"
     CDS             complement(2234..2845)
                     /locus_tag="OQL90_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1369227.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTAMASINLIQLKLQAG
                     RNLTQAETSRLNTVLDYIDAVTATDTSTAPDVIWPELLDAHNM"
     gene            complement(2845..3627)
                     /locus_tag="OQL90_002490"
     CDS             complement(2845..3627)
                     /locus_tag="OQL90_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCS1369228.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDD
                     GRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTILKQITPLSPSIDF
                     DNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
     gene            complement(3631..4215)
                     /locus_tag="OQL90_002491"
     CDS             complement(3631..4215)
                     /locus_tag="OQL90_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCS1369229.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMQELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(4206..5264)
                     /locus_tag="OQL90_002492"
     CDS             complement(4206..5264)
                     /locus_tag="OQL90_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1369230.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATVASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTTTDDAISSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NITIAKNELAVLGAISWT"
     gene            complement(5251..5676)
                     /locus_tag="OQL90_002493"
     CDS             complement(5251..5676)
                     /locus_tag="OQL90_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCS1369231.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLRRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(5676..6224)
                     /locus_tag="OQL90_002494"
     CDS             complement(5676..6224)
                     /locus_tag="OQL90_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCS1369232.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(6224..7303)
                     /locus_tag="OQL90_002495"
     CDS             complement(6224..7303)
                     /locus_tag="OQL90_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS1369233.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARKRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(7300..8628)
                     /locus_tag="OQL90_002496"
     CDS             complement(7300..8628)
                     /locus_tag="OQL90_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCS1369234.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(8689..10524)
                     /locus_tag="OQL90_002497"
     CDS             complement(8689..10524)
                     /locus_tag="OQL90_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1369235.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(10666..10935)
                     /locus_tag="OQL90_002498"
     CDS             complement(10666..10935)
                     /locus_tag="OQL90_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1369236.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(10935..11291)
                     /locus_tag="OQL90_002499"
     CDS             complement(10935..11291)
                     /locus_tag="OQL90_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1369237.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNKTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(11291..12787)
                     /locus_tag="OQL90_002500"
                     /pseudo
     CDS             complement(11291..12787)
                     /locus_tag="OQL90_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
     gene            complement(12771..12941)
                     /locus_tag="OQL90_002501"
     CDS             complement(12771..12941)
                     /locus_tag="OQL90_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCS1369238.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(12968..13510)
                     /locus_tag="OQL90_002502"
     CDS             complement(12968..13510)
                     /locus_tag="OQL90_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369239.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHT"
     gene            complement(13507..14013)
                     /locus_tag="OQL90_002503"
     CDS             complement(13507..14013)
                     /locus_tag="OQL90_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369240.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(13988..14398)
                     /locus_tag="OQL90_002504"
     CDS             complement(13988..14398)
                     /locus_tag="OQL90_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1369241.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGSNGDSLFSR"
     gene            complement(14395..14718)
                     /locus_tag="OQL90_002505"
     CDS             complement(14395..14718)
                     /locus_tag="OQL90_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCS1369242.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(14721..14921)
                     /locus_tag="OQL90_002506"
     CDS             complement(14721..14921)
                     /locus_tag="OQL90_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369243.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(14970..>15368)
                     /locus_tag="OQL90_002507"
     CDS             complement(14970..>15368)
                     /locus_tag="OQL90_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCS1369244.1"
                     /translation="ADAIIKLIYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWR
                     PGLELGQPSSLAGYGIAENEQMPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTN
                     KPFVGFYTTKRTGGMLVDSQAIKLLKIAVA"
BASE COUNT         3541 a         4363 c         3702 g         3762 t
ORIGIN      
        1 gctgaaaatc aaagacccgc actatgatga aaagcacctt gctattgagc aggcgctgac
       61 tcaggggcag acgaaacatc ctgtgtttta tcaggatgaa gtcgacatcg acctgaatac
      121 gaaaatcggc gtggactgga tgcccaaagg gcaacagaaa cgcatcgcta cgccgggaca
      181 gaacctgaag cattatctgg ctggcgcact gcactcaggt acgggacggg ttcactaggc
      241 cagtggcagc agcaaaagtt ctgatttatt tatcagtctg ttagaggcat tacggcgtag
      301 atatcggcgg gcgaaaacca tcacactggt ggcggataac tacatcatcc ataaaggccg
      361 taaagtggag cgatggctgg aagaaaaccc gaagttccgg ttgctgttcc tgccgacgta
      421 ttcaccgtgg ctgaatccga tagagcaact ttggttatcg ttacacaaga ccataacgcg
      481 aaatcatcag tgccggtata tgtggcagat actgaaacag gtaacgcagt ttatgaatgc
      541 tgctttaccg tttccgggta atcaacacgg tatggccaaa gtggagcggt aatatgcgaa
      601 gctatttaga ttcagtttga atctcagccc tgacattcat tctttaccga cgttccatta
      661 gcccaaatct gagtatataa cctcaattca actgactaaa tttaacacaa tatcaatgac
      721 aaatgtcttt accattggta catacaattg caaaatcacc atctctaagc aataagacta
      781 cgcatattaa agtgagttat attaaatgtt tgaaattgat agcctattat taataacatc
      841 cgtgataatc ttgtcattat tggctgtaaa attatttgat gaaatttctc ctatacaact
      901 tgtcgatcat ggaagaaata atcagataga tggaatgaga ggattcttag ccatcttcgt
      961 acttattcat catgccgcta tttggcatgg ctacttatta accggggtat gggaaactcc
     1021 gtcatcaaac ttattaacaa atttaggcca agttggtgtc tcattctttt tcatgatcac
     1081 cggatatcta tttttttcga agattcgctc aagtgaccag gattgggtaa gactatatat
     1141 atcgagattt ctgcgattaa ctcctatgtt tattgtaagt ttatgtttag ttcttcttgt
     1201 tataggtttt aaatcaagat ggagcgtaca tgtctcccct gcctctctta ctgtgtcact
     1261 catgagatgg gcaccattca ccgcattagg gatgccaaat attaacggtg ttaaggactc
     1321 attcactatc aatgcagctg taacatggac cctcgtttac gaatggtttt tttatttttc
     1381 attaccggtt atagctgcac tcttcaaaag aagagtcagc atccacatga ttataataag
     1441 tgttttagtt ctagtgattt tcttatgtta cccatcaaaa aaaatacatg ttatttcatt
     1501 tttatttgga ctgttagcct cttattcaaa taattatgta catagagttg ctaaagctaa
     1561 aataacgcct attatgataa ttgcattact aagttacgaa atgatatatt ttcctacaac
     1621 ctacgctcca ctccctctca taatttgcgg tgtttgcttt atacttatcg catcaggctg
     1681 tgatttttat ggtatattga gattaaattt aacaagaaaa cttggtgaaa caacatatag
     1741 tatttatctt ttacatggag tattccttta ttgtgtaatg acatgggtca tccctagtaa
     1801 ttattctaat tattttttta ttatgttaat aagcattact gctttttgcg tcacactttt
     1861 atcatgcata acatttaaat taattgaact acctttcatt aatataacaa aacaaaccgc
     1921 tatgaaaatc aggggcataa taaaaaacac ttagtaatca attgtcacct aatcaagagg
     1981 tggtttatag ataacaataa agaaccttcg ccatttccgg aggttctttt atatccttat
     2041 ggtaaatata aattataaat attattttta tggtgttcca ctatgaacag ggaagctaaa
     2101 taatgattac aaaaatatgt aacttcaggc aatgcagggg ctatgttatt gccttaacac
     2161 cataatgagt aatattgtgt cccgaggata aaagtactgt taattcttat tgatgcttca
     2221 atagcactca aaatcacata ttatgtgcat ccaacaactc aggccagatg acatccggcg
     2281 cggtgctggt atctgttgcc gtcaccgcgt caatgtaatc cagcaccgtg ttaagtcggc
     2341 tggtttctgc ctgcgtcaga ttccgtccgg cctgtaattt tagttgaatc agattgatgg
     2401 aagccattgc agtatcaatc agcgactggc gttgtgcttc tgctgcatcg actgcggcgc
     2461 tatgttgtgc ctcggtatcc gtcacccatt tctcaccatc ccatttatcg tatggtgtta
     2521 acgggacgat agtggttgta ttatcagggt aatcacccgg agctgtgatt tcttttgatt
     2581 ctcctgtttc ggtgctaaag acgatttcac cgcgatggtc tggcacatat tcccatgagt
     2641 taaaatctgc agaacggcag attgcataac cagctttgta tgcgcctgga gcatctaaac
     2701 aggaatatgc cggaatgccg acaccaatag caagatattc agttgaagct gaaatatact
     2761 cccgactcat gacatcaaag ttataaacag taatttctcc tgccatagtg gcaattaatt
     2821 cactgtttaa ttctgcgtta ttcattatgc agccctcaca atatagttaa aagcgacatt
     2881 tcgtgggcgt gtttcattcg cacctgcagg tttagtcgtt gccgcaacaa caccaccctc
     2941 tgtaatagcc gggttgggaa ttgggtaatt atcgaagtca atggacggag acaggggcgt
     3001 gatttgtttt aaaatcgtgg tgcctgtaaa actttccaga gtctggtcgt tagttcgtga
     3061 agtccagatt ccccaatagt ggttatgact gacaatagca ccgtcctgaa gcgttaataa
     3121 tccccttccg ttatccactc cacgcccgtc atcccagccc cgaataaact caccgcgtaa
     3181 atcaggtaat ttatttgtcg gataagcctt tgccagttcc gggtattctt cagcagaaaa
     3241 agctgctccg ttgcatttca gccagcctgt tggcggagtg gctgaaggcc acggaacagg
     3301 cacaccaacg ggtaatgctg agccttctcc caaaccaagg ttttcgagag ctgttttcac
     3361 cgtgccatcc gatttgatat cgccaaacgg attcttgcgg cttaacagca gcgcacgaag
     3421 cgcggtaagc agctggtcat gccgcccctt ctccaggctg gcaccggatg cctccacaac
     3481 gctgcaaagc tcctcctgca acatgtcaaa gtagtcatca tccagatcgg tggcaggcgt
     3541 gccggtctgg gggttaccac gggtaaaacc gttcttaccc gcgccgaact tatccttctg
     3601 cgcggttttc gtgtctatac gatgcatgga ttactccgga tatttaaaaa tcacataggt
     3661 atgcgacggg cagagtttgt taagaacaca ctcgacaacg gtgtcccccc agaagcgcag
     3721 cgcggaatca cagggatcgc cacatgtcat ccaggtggtg ttggtggcgg ctggcatgtt
     3781 gacctgccag taataccgcc attccggcgc attcaccgcg tcagtacagg cagatgagca
     3841 ggtgaacgtg cttttgtcgt atcgcgtgat ggtggcgtct ggtctgccca aggcagcaag
     3901 ctgtgcaagg taaaaatcct cattgatgcc gcccgccagg ttaaccttcg catccagccg
     3961 ttgctgacgc tggcgaaggg tctgcgtccc tgccggaata cattcatccg gcaggccgca
     4021 cagacgctcc cagcggttta tcagttcggt ggtggtgcgc ggatccagct cctgcatcag
     4081 ggcatccgca cgctgatgaa cacgggttaa tgacggtgcc gcaccggcaa tcgccggatc
     4141 gctggctgac cacgccggac cgggcggcaa cagtgctgac aacagacgga tgtaatcatc
     4201 gtttgtcacg tccatgaaat cgcccccaga accgccagtt catttttcgc aatggtgata
     4261 ttgtccgccg gtgcaagcaa ctgatggctg tattccccgt tcgcaccgga aatcgcttca
     4321 ctgatacgtg acaccttcag ttctccctgc ggataaccat cacgcagcag gaacgaacgc
     4381 aactccgcgg tgatggcagc ccgtatttcc ggtgtgtccg gcgtcacacg gatatgaaaa
     4441 tccaccgtat gtgccaccgg cctgaacaca tacaaatcag agcctgccac cggggccagt
     4501 ggcccgatat gttgtcttgc cgccgtttcc gttgattctt ccggaatggg attaatcagg
     4561 tcactgctgg caatcatcac accgacagtc cccgttccca tccagtgtcg gtatgtccat
     4621 gcgcgggtaa tgccaggcac ttctttagcc cagacgacat agtccccgtc agccccgccc
     4681 tgcggcgtcc agtaataccg ctcaatgacg cgggcgcgcc acgtttccag ctcttcagta
     4741 tcaaatccgc ctgtcagggt gtcagccaca ccggaagacg gcagaccatt caccggcgtg
     4801 accaggatta atgccgtacc gtcgtcagcg ttaccgaccg cacctgcact tgagcaggcg
     4861 atcggcacgc gcaggacacc accggagctg attgcatcgt cagttgtcgt gtactgcacc
     4921 aggtcatcgc gctgaataac actcccggca gtcaccttca ggccatcgct gacaccttcc
     4981 cagcgcatat acccgctggc aaccgtggcc cccttgcgcg gacaccgttt catcgcagca
     5041 tgtcgcgcca gccaggactc atcgcacagg tcaggcagca tattcattgc cagataatcg
     5101 atgtaaccgt agaccgtatg cagcgccgcc gcatacacct ttgcccgcac gtcttcatcc
     5161 atgcgccgga gcgtgtcgct gacgtccagc ctggcgaata aatcgttacg gagcatactg
     5221 atattttctg ccagcgtcgg gcgctgaaat tcactgtccg ccatgcgtta tcgcactcca
     5281 cagatcatca aaagaaatca ttactggtcc atcacgacgc ctgagggtga tactgttacc
     5341 cagctcatta atcccggtgc ggcggatatc cagatcaata cgggacacca cgccgtcatc
     5401 agtcatccat tgcaggcatt cgcggatata tccccttacc gtctgcacca gctgattggt
     5461 cagtttgctg cgctgaagca accacagtcg ggagccgtaa cggtcattct gtaccgcagg
     5521 ccaggtatcc ccccaccatc ccatcgggac gtcggcattg tcatcaggct ccgcccgccg
     5581 ccaggtgaac agggaaatca ccacggcgcg ggtcagcgga tccagcggtg cgctggcgca
     5641 ggtgcgttta ccgttcaccg tcagccacag ttccatcatg cctccatcgc tttatcaggt
     5701 ttgtcggtgt tactgccctg accgttctct ctgtgacgat gcccgttata ggcaagccgc
     5761 atcgctgaca tggtggtgcc gccggagtcg cacaggtctt tcacctgtcc ggtcacttcc
     5821 agatccattt caaaacgtgc ttcaggtgca ttgcgaaacg tgatcgtttt ccctgcaccg
     5881 tccaccacga tcccctcccg ggtcagcgtc acagactgcc cctgatcgtc atagacagcc
     5941 acctcacccg tttgcagccc tttcaggcgg tagcgccggt ccgacaccgt aacaaccacc
     6001 gcatgagaac ggtcgccatc cggaaacaac accaccgctt ccgcaccgct gtttgccctt
     6061 gcggtaaaac cgtagggttc aagatgttca accccggctt tgggttcacc ggcaatcagg
     6121 gacacatcca cggtctgaca tttcgtggcg gcactgatgc ttttcaccac tgcccgccca
     6181 atcaggccga gaagttgtcg ctgcatggct tcaatcgtcc tcatcagaac gggtcctcct
     6241 gtactctggc ttttttcttt ttccgcgcgc cgggggcttc gggttcaggc agataagcgt
     6301 caggcgggcc gacacggatt tccgtcaggg tgccgttctg gtcctgagta aacgtgactt
     6361 ccgagacaag cagttcggta ttgtcgaaac cacagaccgg atcgaagaca atcacccgct
     6421 ggttgggctg ccacagcgta ccgttaccct gtcgccagcc ctgcaccaca taggtggttt
     6481 catccgtccg cgccgcccgt tttcgggctt caaagtcagc acgcgcaata cagcctgccc
     6541 ccgtggcctg ccctgtctgc ctgatataca tcggacggta acgggcaata aatgcgtcct
     6601 ctgtgcgggc ccgcagcgcg gtggtggtgg cctcaccgaa atcatcgtcg tttccggcac
     6661 gctgccccgc cacctggtaa acagaaaacc gctcccggat actcttctcc gtatcacagg
     6721 aaaggatgtt ttccccaagt accagcgcgg tatgtgcccg cgttgagcca ataccgccaa
     6781 tcaccagcct gccgtgcggg tcgtcgtaag ccagcgcctg ctgctgaccg agtattttgt
     6841 tgattacctc aatcaccgtt tcaccgtgat caggctgaac atcaggaata acacccgacg
     6901 gcgcaccgct gttcaccacc tcaatgccga aaggcgcagc aagcgcctgc gcaatctgca
     6961 ccagcgagcg tccgttaaac tgtgtcggtt cggctgcaca gtcaatcagg tcagccgtca
     7021 gactacgtcc ggcaataccg gtgctgaccg aacgggcatc gtaacgaacg ggagtcgcct
     7081 ccacccagcc ggtgatcacc agctcatcac caatcagcac ttccactttt gaaccgtttt
     7141 taatgcgcgg ctgaagcgtg gtgataccct catctcccgg ccattggcga gtgatctcca
     7201 cactgaaatc ccgcgccagc cgttcaatac cggcaccgat gcgcaccgat gtccagccat
     7261 tccactcccg gccatttacc cgtagcgtga cattgtcgtt cattgcactg gcaccttcag
     7321 agggatcacc ggcacaaagc cgggatgcgt aatggcatta cgccgaataa tgtccgcgtc
     7381 acgcgccgcg ttatcaaacc aggtcgccgc cagcaccagc gcgggtaaaa cctcatccgg
     7441 cgtgcgctga atgatccgtg cagactgttc aaggcgcgtg ttgatatccg cattcagatc
     7501 tgctttcacc cggcgcagcg ccagaaacag cgcatcactg gttgtacggg acaactcctt
     7561 atcaattgcc gtattcagtg tgtcgcgaat gtcggtcagt tcttcccacg ttggcaggtc
     7621 aaccgtgttt ttcaccgccg gtgcattgtt cagtgccgga tgcgtgacag aaggccagcc
     7681 ggtgctctgc gcgggtgttg ttgactgccc tactgtggca ttctgcatca ccgcggaagt
     7741 tgtgggcgca ggcaatcgtg tgacggcata cgccgcttcg ctgattgcgg tcgtacgaag
     7801 ggtgctggca accacgttac gctgctgcgt cgccgtggcg gtagttttac tgtccgtttt
     7861 ccagacgccg cgcggttgca gatcgctgcc gaggctgaca ccggaaagcg ttttgatcat
     7921 ggtgaccagg tcgctggcgt taccataaag gcgtttcccg gtacgccaca ttttctgcac
     7981 ctgctcaacg aaatttttgc ctgacgatgg tggcggcaga agtaccgaga tatccccctg
     8041 caacagcctg gcggcatccg atacggcaga atccaccact ttcatcgcat cagaaacata
     8101 cccaagcatt gtgctggcat taccgacgac gtcgttctgc acaaaatctg ccacgccatc
     8161 gatactgaaa ccactgaagc tgtcactgat gcagtcatcc agtgcagaac aggatgacat
     8221 cagcgtctgc gccgtcgccg cacctgaagt ggggtaagag agttctcccg cttcgacaaa
     8281 cttcaggtca aagcggacaa tacgcccttc actcttcgat gtgctgaccc gaacttcccc
     8341 gtcaacacag actttcagct caccgtaagt cggatggaca agcgtgccgg gaccgggttt
     8401 attcagcgcg tcaatcaggc gatcgcgctg gtcaaagcag tcatctccca ccacataagc
     8461 tgtgatggac gggcggaaag tgattttccc caggtcttcg gtatagggtt tgtcgcggtt
     8521 cgggtattcg tgcgtttcca cacgacgacc ggttcccgca ctttcttctt caaccttaaa
     8581 cggcacaccg cgaaatgacg cgtcctgaag tctgtctttc cacgtcatat aaactccgta
     8641 cataaaaaat cccaccggag tgggactcat taacagatta atttttcatt acctgccaaa
     8701 gcgcgtatag ccaacatcat ggctgacatc aaaaccgctg gatcgcgttt ccataacccg
     8761 catacccgga ggcgaattca caaaagagac cttgatctca ccatcaactt ttggcgcaga
     8821 agctttgtta atcatgaagg gattcgggcc tgtggcaccg gaggcgttgt ttgactgagc
     8881 cggatctacc gcctgataag gtgtgtatcc ccgtgccggt attcccgtcc cataagcatc
     8941 ataagcaccc gcgccccact gcgcagagtt aatggcatcg accgtgtcac cggaactgtc
     9001 ggtaaaccac tcaataattg gcttcagttt gtcccacata tcctgaaacc acttaacaac
     9061 cggtccccag ttattgatta ccatccccag cggcgaccag gcaaaaacct tcttcagaag
     9121 ttcccagcca gcctcaaaat aaggaccaat ggtttcccag agcttcttga aataaggtcc
     9181 gacaacatcc cagttagtga taattaatcc cgcagccagg gctatcgccg tcgcaatcat
     9241 tccaatcggc gtcatcgaca tgatcctgct gacaatactg atggcaccgc caacgcccat
     9301 caatcccagt ttcagaatcg caagaccggc agcaagcccg acgacgccgc gaataacccg
     9361 gggattttca tccgcaaact tcgtgaattt ttcccctaac tcccccagcc attgcgtgat
     9421 atttttggcg tcaccagaaa atgcgccgcc aatagccgca aggccgttag ttgcggtccc
     9481 cgtcattgcc tcccacaggt tggacagcgt accaagctgg gcctgaacac gtttattcag
     9541 gctggcctgt ttattcatct tctgctggat ctgatcgtag ccatcctttc ctttatcgat
     9601 tagtgcattg accacctgaa gggtttcggc atcatcacca aatattgcct taagtacacc
     9661 tgttcgctta acgtcggtca gttttcgcag ctttgccagt tgcctgaaca tgttatcaag
     9721 accgccaaaa cttcctttgc cgtcagtaaa atcgagctgt accccgagtt tctggcgggc
     9781 catgatttta ttgacgtccc tgattttctt aacgcttaat ccggactgga taacttttcg
     9841 cagggcgtta cctgccgact ccccgttcat ccccatctga tccatcatga cgctgatggg
     9901 ggcaaggctc tgtgcagcct gaagaccgtc cttgttcacc atcttcagaa cagagctggt
     9961 tttagtgaag aaggacaaca tgttggtatc gtcaacaccc agataaaacg ccttctggat
    10021 agtgtcgaac agccccatca tgtcttctga cgccgttccg gtagcatcct gcatctttgc
    10081 agcaaactca gcagccgctt ccggtgtttt tttcagttgt accgcaagat aagctgtcgc
    10141 tttacccaca ccgccaagaa tgttttctgc cgggatcccc tgacgcacca gcatctgcat
    10201 catgttctgg aaatcagccg ttgtaccggg tagctggtta cccaggccaa tagccagttt
    10261 attgatgtcc tgaaagctct ttccgacctc gccgttcgca tccatcatgg cgactttcag
    10321 cccggtggcg gcgttttcct gatcggcata agatttcagg gaaagcgtca gacccgctgc
    10381 cagtccgcca ccaagcgcca gcccaccctg tgacgcttct tccgcctggc gtttaaatcc
    10441 ccggattttc ttttgcattt tcgacagcgc gggagaaagc ctgtcgacac cggtgatcaa
    10501 cgccttaagc tcaaattccg ccatgtgtgc gtttctcctg ctctatcctg tttgcctgac
    10561 tgaccagcaa ggtaatttca ctgatcggca tattcagcaa ttcgaaagga ttaatgcgcc
    10621 agtaactggc gcagtcaaag aagcgatcag tgaggtattc agccgtcagg cctggaggaa
    10681 aaaaccagcc acaagccacg ccgctgcatt caggtctgcc ggagacatct ggtcgacaga
    10741 gctttgcggc actttcgcca gccgcacaat gtatttcgac accacatgcg ccagaagtct
    10801 gactgactca tcctgattca tctggtaggg ataccccagc tcgcggacat ccttcccggt
    10861 gggttcatca aactccagta cggagagtgt ctcgccatga gcagtaatcg gtttctttaa
    10921 ctcaagctct ttcattactg gtaatcccct tcttcaccgt ggaactcaag atcaaccgtg
    10981 ccttcttcgg cattatggtt cgcttcgccg tgcagccagg cagacgacag tacatagacc
    11041 tgaccgttcg ccagctcggc agtgatggtc atctcatcag acgaggtgat tttgcttacc
    11101 ggaaaattct tcggcacctt gaaggtccct ttgacataag gcgcacggtg agtttccttg
    11161 cggtccactg aaccgtccag gccgatgatg tcatcgttaa ccgttttgtt catgggcacc
    11221 tcaatgccgc cggtcagcga tagctgctga ccgtcaattt tgaaataaca ggttcccccg
    11281 atacgggcca ttatgcagac tcctctgaat actgaagacg gaactgatta accacggcaa
    11341 agacacgcag ctggttaaca tagtcaggcg ggaacagcgt gttcaggcgg ttcggatcgc
    11401 tggcatcacg ctccacaacc aggtactgct taaacagttc gtagttttcc acgatccccg
    11461 cacgctcaag ctgacggtag gttgccagca gttccccttt gatcaccgcc ggggtgacaa
    11521 tcgcctgacc gggaccaaag cgggtaccgt cactggcaag cttgtgacgc ccgtacttac
    11581 tggtaatgac ggatttcagt ttgcgcagta catacgcgct ggtatgcagc gtctcactgt
    11641 cgaggtaact gttatccgca accccgtaag cgtttttcct gtacgtggtg acatcacgct
    11701 gaatgcgtag taccccgctt tcgacatacg ccgttgccac gccatgagac agcagggtct
    11761 gttgttcggt catcgtgaac cgtttcccct tcggcgcagg cagcataccc accagctcac
    11821 cggtctgcgt gggacgtgcc ggatcgttgc ggataaacac cgctgcgcgg gcggtacggc
    11881 ttgccgccag ctcgtcggca ggcgtctggg tctctttttc gtaccccgcc agggtaatgt
    11941 gctgctggtt aaactggtca cctgcggtca ccagttctga cagcgtgccg atctttgccg
    12001 tatacacatg accatacagc tgacgcgcat agctccagcg accgctggta tcgttcatct
    12061 cggtcaccag cgtgttaacg gaggccgtgt cgttgaacgg caggccgata taatcaaacg
    12121 gctcatccgc cattgcagcc accgcgccgg tgagaaccgg agcacccgtt ccggcggtac
    12181 ccgtcgccac ggcaatctgt acgcccgctg gcagcacttc gcccccacca aagccgtagt
    12241 aattgaggct gacaggaatt tcattcccac aaagcccctt atgacgcgcg gtcagtgtga
    12301 ccacgcctgc cgaagatgaa gccgtaaacg gcagggtcgg aacggcattg atggcatcct
    12361 agatactgct ggcaatcatc gtgacgttat cgccgttagt caccggtgcc tgcacgcggg
    12421 tacgtcctac atacacattc accgtgccgg tttcggttgc cgccccggtc accgtcagcg
    12481 taactgttgc cgccgcacct gtggattcag gaacggcaat cacatacagc tcgccaaacg
    12541 ggtcagtctg gcgataagcc tcgaccatac gcgccagctg acttcccgca ccacaaatct
    12601 ggcgtgcata gtctgccgac gacatcagta ccagactgtt ggcaacaatc tctgcaccgt
    12661 tattggcatg accaatcagc agcgatgctc cgctgtcctg tgcagtattc gccgcctggt
    12721 tatccatttc cgcataaaac aacggaacca gcgtattcga cggaatggtg ttaaagctta
    12781 tcgtcatcgg tgttcacctt tttattcacg cgccggatat cacccgctgc ttcacggcgc
    12841 agccagtagt tgttctcgtc aacatttcgc ccttcggcgg gcaaaaggtc gccgcgggca
    12901 gggtcaggca ctgaccgccc tttaacaggt ttgacaaaca tgaggatcct caggaaggaa
    12961 gggttattta ggtgtgatgt tcgatatcgc cgtcaggccc gttaccgggc tcgagataat
    13021 caacatcaat cgccagcgtt tgcagttcat ccagactgtt cagatcatcc tgctggcggg
    13081 tatcgtcttc agtcagctcg ctgatgaccg aaaaatcgaa ctgataaatc agctcatgac
    13141 gattcagatc cagcagcgtg ccgccgtcat aggtaatcgg gttaccgcac gcctccgggt
    13201 tccagcccag cagagcctta aagagcatct gccggacatc gtccaccaca tcatacgagg
    13261 caaactgacc acgctcatca cgcccattac tcagtatgac aaccacggag aaaccctctt
    13321 tcagctcctg ccagtagtcg gtctggcttt tgttttctcc cggagaatca tcacccggta
    13381 caacatatgc cgccgggagt ttcagctttc cgacctccgg cagatttttg aactgggccg
    13441 cgcctgcaac ccggttttca aaatacggac aacgggcacg cagtgcagca ataacaggag
    13501 tcagtttcat ctgtgtcgtc gctccggctt cagtgattta cgcaattccc gcgccagaaa
    13561 atagcgtgtc cagctgcggt tcttttcaag cgtttccacc atgaagttat tacgtggagc
    13621 cagtcgccag ccgctgccac cggatgcacc acgatgatga ctacgacgac gttttgctcc
    13681 tccccggaca ccaaaaaaca gaaacgccgg atagaagtca ccagagatca tccggttccc
    13741 cttcccgttg cgctggttag gggcaatgcg tgtcataaaa ccggctcgct ttttactggc
    13801 tctcggcacc atataaccaa tcgaacgagc caggcgtccg gtctgataac cggggttttc
    13861 acccggtgcc gaccgcgcac ggcgcatcac cagccgacgg gcatcacgca tatgacgctg
    13921 cccaatcgtg acaaacgccc gccggacacg agcgcggtta aagcgcatct cggcgggctg
    13981 ctgaacatca acgtgaaaaa agggagtcgc cattgctgcc tccgtgactc tgcctacatt
    14041 cgcccagctc cgtacactcc agcagcagaa agcgccgcgc cccgttcaga tcacgttgac
    14101 gtttcacccg gtacacactg tcatcacaga ccacctcata atcagcagtg atcccccggc
    14161 ggtaacgaat ggtgatgtaa tgggtgatgg cgtccccggt ctgcgcggtt tcctgccagg
    14221 tggtggcact ggtctggata accttcgccc atgtccggaa cgtaaccggg tattgaggct
    14281 ccacgccaaa gttatccgcg ggcatatcca cccgctggcg gatcaggacg cgtttattca
    14341 gttcaccggg gtccggcaga atgtaggttg cgctggtctg cgcctgacga attttcattg
    14401 cggaaagtac ctgtacgggc cgacaagcca gccaaaactc tgcggcatgt cgagtttctc
    14461 cacttccgta accgacgagc ggttttcgta aaaatggctg ataagcatca gcatccccag
    14521 acgaatatca tccggcaggt gcagcccgtc cggatcgctg tccggaatgg tttcatccgg
    14581 tgcatagagc ttccggttca gatacgtttc cgtccgcttt tgtgccgcac aggccagcag
    14641 ttgcagatgg cggtcatcag catcgaaatc ctcatccagc cggagttggg ctttaatctc
    14701 ttccattgtc agaagcatac tcagccctct ttactggtcg tggctttttt ctcttttgtc
    14761 gctttactgc tttttgcact ggttccgcgc tctgctaacc cggcctgaag tgcaatctcc
    14821 tgcacccggg caggaagcgc cccgtcgtca tactcaccgg cccgaatgac ctcaacacgc
    14881 ataccgtccg gtgaccattt cagatcttgt ttcaggatca tgattcttca cccgtcagaa
    14941 caggggcgcg gttccgcgcc cctgagtgat tacgccactg caatcttcag cagtttgatg
    15001 gcctgcgaat cgaccagcat gccgccggtg cgtttggtgg tataaaaacc gacaaacggt
    15061 ttattggtgt acgggtcacg cagaatgcgg gtgccgatac ggtcaacgat ggtgtaaccc
    15121 cgtttgaagt taccaaatgc aatggctttc gcatcagcgg cgatatccgg catctgttcg
    15181 ttttcagcga taccgtaacc cgccagagag gacggctgcc ccagctccag ccccggacgc
    15241 cacagatagt taccctcgct gtctttcagc agacggatgg caaacaggct attgttgttc
    15301 atcatgaact tcgcgccggt gcggtgtgcc ttacgcagcg tgtaaatcag tttgataatg
    15361 gcgtctgc
//
LOCUS       DAJVOY010000092        15302 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVOY010000092 DAJVOY010000000
VERSION     DAJVOY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.092"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..311)
                     /locus_tag="OQL90_002508"
     CDS             complement(<1..311)
                     /locus_tag="OQL90_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS1369245.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     G"
     gene            complement(316..501)
                     /locus_tag="OQL90_002509"
     CDS             complement(316..501)
                     /locus_tag="OQL90_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1369246.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(498..3137)
                     /locus_tag="OQL90_002510"
     CDS             complement(498..3137)
                     /locus_tag="OQL90_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1369247.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3345..4334
                     /gene="ldhA"
                     /locus_tag="OQL90_002511"
     CDS             3345..4334
                     /gene="ldhA"
                     /locus_tag="OQL90_002511"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1369248.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4445..4867
                     /gene="hslJ"
                     /locus_tag="OQL90_002512"
     CDS             4445..4867
                     /gene="hslJ"
                     /locus_tag="OQL90_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCS1369249.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4864..5130)
                     /locus_tag="OQL90_002513"
     CDS             complement(4864..5130)
                     /locus_tag="OQL90_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1369250.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5404..8928
                     /gene="nifJ"
                     /locus_tag="OQL90_002514"
     CDS             5404..8928
                     /gene="nifJ"
                     /locus_tag="OQL90_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1369251.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9294..10427
                     /gene="ompN"
                     /locus_tag="OQL90_002515"
     CDS             9294..10427
                     /gene="ompN"
                     /locus_tag="OQL90_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCS1369252.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10568..11002
                     /gene="uspF"
                     /locus_tag="OQL90_002516"
     CDS             10568..11002
                     /gene="uspF"
                     /locus_tag="OQL90_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1369253.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11559..12473
                     /gene="sitA"
                     /locus_tag="OQL90_002517"
     CDS             11559..12473
                     /gene="sitA"
                     /locus_tag="OQL90_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1369254.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12473..13300
                     /gene="sitB"
                     /locus_tag="OQL90_002518"
     CDS             12473..13300
                     /gene="sitB"
                     /locus_tag="OQL90_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1369255.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13297..14154
                     /gene="sitC"
                     /locus_tag="OQL90_002519"
     CDS             13297..14154
                     /gene="sitC"
                     /locus_tag="OQL90_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1369256.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14151..15008
                     /gene="sitD"
                     /locus_tag="OQL90_002520"
     CDS             14151..15008
                     /gene="sitD"
                     /locus_tag="OQL90_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1369257.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         3844 a         3847 c         3703 g         3908 t
ORIGIN      
        1 ttaccgttta tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg
       61 gcgagtttcg cgatatcatc tacagacaca ttattttgct ttgccagttg ttggtaactc
      121 gctttacgtt cggcattaat gtcggttact aatttctctg tttcagcatc cgttttcagc
      181 gcaaccagat aaccataaaa tgtttccccg acccgcccct gcgttctggc ttcatccaga
      241 gtcaatgcca ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt
      301 ttcttcatca tcgcctcagt aaagatcgct acgagtttca agcagctctt cgacatcttt
      361 gtctgccttg atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc
      421 cttgggcgca gcgacttcaa tacgcggcgt acaggcagcc agcataaaag atgacgtcaa
      481 cgcagccagt aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt
      541 cccggcagac gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg
      601 cgccacagcg taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta
      661 ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct
      721 aaattaatat ttgtcgatga acggctaatt tccatatagc gcaaccagtt aattgctgaa
      781 cccgcagtca tattgtcttt caccaccgca tctgcggtgt ctttatccag ccgcaaggtc
      841 attggcccgc tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat
      901 aacggcaatg caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta
      961 accagttcgc tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata
     1021 cgtaattgtt tcatcagtac gttaccgcct aacacatcga cgctaacatc agtcaacagc
     1081 aagggttctt cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct
     1141 gtcaccagat taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc
     1201 caggctccat cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct
     1261 ggcatccagg cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca
     1321 ggcgcagcag aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag
     1381 tcgggtggca ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg
     1441 cgcagacgaa taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt
     1501 aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat cgacgctaaa ttttaaggtt
     1561 gatggcggta acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg
     1621 ccgctgaagg agggatgctg tgcgtcacgt acccagacga cgggcttgtc cagaattaat
     1681 cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagaggtcg
     1741 gtcagcgtaa tcgtgctgtc atgccattca ccttttcctg cgacatccca ggtggcattc
     1801 atcggtgtaa aactgccttt tccccagtag cgccactgcc agcggcccgc gtcagggaga
     1861 aaatcattcg ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc
     1921 tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa
     1981 ggccagcgga tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac
     2041 gcgcctggct caaaggccag cgttgggtca gtcagacttc cacttagctg cgcgggtaag
     2101 cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagctgg
     2161 ctgttatcca tgcttaattt tcctgggcca aaatttagca ccgcgttgcc tttacccgct
     2221 tgcccctggg tcagcacact cagtcgtccg ctgatcagag cgttctcaag ccctgcctgc
     2281 cagttgtcga ctttgacacc cagtcggcca ctcaaaggaa aacctgcata cggccaactc
     2341 cagcgaccat cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac
     2401 ggatcgccgt tatcacgtgc cagcacaatc aattgcccgc tattttcctg ccagtccagc
     2461 tctgcatcca ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt
     2521 acaggaagtc catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc
     2581 tgatattcaa acgcgatgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg
     2641 ccttgaaatt taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg
     2701 agagagagtt ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc
     2761 caggtgttag gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc
     2821 tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat
     2881 aaccagcggc tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc
     2941 accagataac ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct
     3001 aatgcaatac gtgtcccgag cggtagccag atgcccgcca gcgtgggaac ccacagcccg
     3061 agcgtcatca gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct
     3121 ttatatttac ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat
     3181 acgggtattg tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt
     3241 gttgatgaat ttttcaatat cgccatggtt ttcaattata tttgaaattt tgtaaaatat
     3301 ttttagtggc ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt
     3361 atagcacaaa acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg
     3421 agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg
     3481 aagcggtatg tattttcgta aacgatgacg gcagccgccc ggtgctggaa gagctgaaaa
     3541 agcacggcgt taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg
     3601 cggcaaaaga actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg
     3661 ctgaacacgc catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc
     3721 gtacccgtga cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa
     3781 cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat gctgcgtatt ctgaaaggtt
     3841 ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg
     3901 tggagtatgt cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc
     3961 cgctgacacc ggcaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg
     4021 gcgtgatgat cgtcaatacc agtcgcggtg cattgattga ttctcaggca gcaattgaag
     4081 cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt gtatgagaac gaacgcgatc
     4141 tgttctttga agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg
     4201 cctgccacaa cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca
     4261 gtatttctca gactacgctg caaaacttaa gcaatctgga aaaaggcgaa acctgcccga
     4321 acgaactggt ttaatgttgc agctcccctg caatccaggg gagctgattc agataatccc
     4381 caatgacctt tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac
     4441 cacaatgaag aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag
     4501 taatgacaaa attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag
     4561 cgtaaacggt aagcccgtga ccagcgataa aaatccgcca gaaatcagtt ttggtgaaaa
     4621 aatgatgatt tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg
     4681 tgaactgaca gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa
     4741 tgaactcgat aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc
     4801 gaaccagtta acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat
     4861 gaattaatag ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt
     4921 gggtaatgca cacatcccaa tcgccgtgcc atccagttga cgggcaacag aaagcgaacc
     4981 gccgatcatt gcacaatttg cttctccact actggacatc gacgctttta aacctggcgc
     5041 tacgtgcgcc gcagtcgcct gctgaacagg ttcactacta cacgccgaca acaataaagc
     5101 ggcacaccct acccaaaacg ctgctcgcat cttttcttcc tctgatcttc aagccaaacg
     5161 acaccgccat aaataatagg cagcacagag ggcgacatcg agagctgtcc tgcgcgttgc
     5221 cccgcatttt tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt
     5281 acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa
     5341 agattactac tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt
     5401 catatgatta ctattgacgg taatggcgcg gttgcttcgg tggcgtttcg caccagtgaa
     5461 gttatcgcca tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg
     5521 gccggaaacg gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg
     5581 gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg
     5641 tttacgtcat cgcagggttt gctgctgatg atcccgacac tgtacaaact ggcaggcgaa
     5701 ctcacaccgt ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actctctatt
     5761 tttggcgatc attccgacgt catggcggtg cgccagacgg gttgcgcgat gttgtgtgcg
     5821 gcaaacgtcc aggaagcgca agactttgct ctcatttcgc acatcgcgac gctgaaaagc
     5881 cgcgtgccat ttattcattt ctttgatggt ttccgcacgt cccacgaaat caataaaatt
     5941 gtcccgctgg ccgatgacac gattcttgat ctcatgccgc aggccgaaat tgatgctcat
     6001 cgcgcccggg cgctcaaccc ggaacatccg gtgatccgtg gtacgtccgc caatcctgac
     6061 tcttatttcc agtctcgcga agccaccaac ccctggtaca acgcagtcta tgaccatgtt
     6121 gaacaggcga tgaatgattt ctctgccgcg acaggtcgtc agtatcagcc gtttgaatat
     6181 tacgggcatc cgcaagctga acgggtgatt atcctgatgg gctcagccat tggcacctgt
     6241 gaagaagtgg ttgatgaatt gctaacccgt ggcgaaaaag tcggcgtgct gaaagttcgc
     6301 ctgtaccgcc ccttctcagc caaacacttg ctacaaactc taccgggatc cgtacgcagc
     6361 gtggcggtac tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat
     6421 gtgatgaccg cactggcaga agcctttaat aatggcaagc gcgaaactct gccccgtgtc
     6481 attggtgggc gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta
     6541 tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta cggttggtat ttacgatgat
     6601 gtaaccaatc tgtccctgcc gttgccggaa aacacccagc caaactcggc gaaacttgaa
     6661 gccttgtttt atggccttgg tagtgatggc agcgtttccg cgaccaaaaa caatatcaag
     6721 attatcggta attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa
     6781 gcgggcggcc tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat
     6841 ctcatttccc aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag
     6901 atggctgagc gtttaaaacc tggcggcatt ttcctgctca acacgccgta cagcgcagat
     6961 gaagtgtggt cgcgcttgcc gcaagaagtt caggccgtgt taaaccagaa aaaagcgcgc
     7021 ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat
     7081 accgtcatgc agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc
     7141 gcagaattgc agggtgcgat tgccaaaagt tacagtagca aaggccagga tctggtggaa
     7201 ggcaactggc aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg
     7261 gtaaatccgc acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg
     7321 aaaaccgtaa ccgcagcgat gctcgcaggg cttggtgatg ccctccccgt ttcggcgctg
     7381 ccgccagacg gcacctggcc gatggggacc actcgctggg aaaaacgcaa tatcgccgaa
     7441 gagatcccca tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc
     7501 ccacactcag ctattcgcgc aaaagtggta ccacccgaag cgatggaaaa cgcccctgcc
     7561 agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag
     7621 gtggctccgg aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac
     7681 cgtcagaatc cagagattaa agccatcaat atgatgtcgc gcctggaaca tgtcgaagaa
     7741 gagaaaatca attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa
     7801 cgtattgata ttcgtacatc gcagctgatt acaccgctgt ttgaatattc cggtgcttgc
     7861 tccggttgtg gcgagacgcc gtatattaaa ttgctgactc agctctatgg cgaccggatg
     7921 ttgatcgcta acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg
     7981 tataccaccg atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat
     8041 gccgaatttg gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt
     8101 ctgctggatc aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac
     8161 gctacgccag aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt
     8221 gccgaagcac atgaactgct acgtgatgca gatgcactgg tggaaaaatc aatctggctg
     8281 attggtggtg atggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt
     8341 ttgacggaaa acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt
     8401 caggcgtcga aagcgacacc gctgggtgca gtaactaaat ttggcgagca cggcaaacgt
     8461 aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag
     8521 atttctctcg gcgcgcagct gaatcagacg gtgaaagcga ttcaggaagc ggaagcgtat
     8581 ccgggaccat cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggca
     8641 ctcagccatg accagatgcg ccaactcaca gctaccggct tctggccgct atatcgcttc
     8701 gatcctcgtc gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca
     8761 gaagcactgg aagaaacgtt acttcacgag caacgtttcc gtcgtctgaa ttcgcagcag
     8821 ccagaagtgg cagaacagtt atggaaagat gctgcagctg atttgcaaaa acgctatgac
     8881 ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata
     8941 aggattatcc aattctaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt
     9001 tggaaaatca gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca
     9061 tataacccgc gcagaataac gtatagaaaa ttaaaagtat aatttttatt ttgtatgtat
     9121 tatcttcgat acattcacca gacttattat catttcagaa gaattatttg attaaggttt
     9181 tacttaaggc gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt
     9241 cactccccgg aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa
     9301 gcaaagtact ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg
     9361 aagtttataa taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt
     9421 atttttctga taattcagcg aaagatggcg atcagagcta tgcgcgtctg ggttttaaag
     9481 gcgaaaccca aattaacgat caactcactg gctacggtca atgggaatac aatattcaag
     9541 caaacaacac tgaatcttca aaaaaccagt catggacccg tctggcattt gccgggctga
     9601 aatttgcaga ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg
     9661 aaggctggac cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact
     9721 ttatgactgg tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg
     9781 taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc
     9841 aggaaggcac caacaacgga cgtgatgttc gccatgaaaa cggtgacggc tggggttttt
     9901 ccacaacata tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc
     9961 gcaccaatga ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg
    10021 ctgggctaaa atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta
    10081 atatgacccc gtttggcgac agcgattatg ctgtggcaaa caaaacccag aattttgaag
    10141 tcactgccca gtaccagttt gattttggtc tgcgtccggc agtctctttc ctgatgtcta
    10201 aaggccgtga cctgcacgct gccggtggtg cagacaaccc ggcaggtgtt gatgataaag
    10261 atctggttaa atacgccgat gttggcgcga cttactattt caataaaaac atgtccacct
    10321 acgttgacta taaaatcaac ctgttggatg aagatgacag cttctacgct gccaatggca
    10381 tctctaccga tgatattgtc gctttaggtc tggtttatca gttctaaatc ctcctgcccg
    10441 ctgttatggc gggctttttc tgcttattct tcctcctttg atctaaatta aaaatgtgaa
    10501 ctccatcatt acacaaaaag tgtcatctgg cgttacactt tatgcggata ctaaaacagg
    10561 aggttttatg aacagaacga ttcttgtccc tatcgatatt tccgattcag aattaactca
    10621 acgcgtgatt agccacgttg aggcagaggc aaagattgac gatgcagagg ttcatttcct
    10681 gacggtaata ccttcactgc cctactatgc ctctctgggt ttagcgtatt ccgcagaatt
    10741 accggcaatg gatgacctga aagcggaagc caaatcgcaa ctggaagaga tcattaaaaa
    10801 atttaaactg ccaaccgaca gagtgcatgt ccatgttgag gaaggctcgc ccaaagaccg
    10861 cattctggaa ttggcgaaga agatccccgc tcatatgatc atcattgctt cccatcgacc
    10921 ggatatcacc acttatctgc tcggttccaa cgccgcagct gtagtgcgtc acgcagagtg
    10981 ctccgtgctg gttgtgcgct gacactaacg cccgcacatt gctgcgggct ttttgattca
    11041 tttcgcaaat gtgctgacat tttcccctct aatccgtacc atacacgcca ctgtttttat
    11101 atcagatttc ttatgctggg cattccggca tgacgcatac tcttctgatg ccatataacg
    11161 aattgagtcg cttttaaatg tcgcaaaatc aagaaattag taagaaagaa caatacaacc
    11221 tgaacaagta agggcaaaaa tcacaactat ctgactcata agtattttat ttatttttca
    11281 atatgttaaa cattatcagc gacaacaata agctacgata acccactctt tttttgcccc
    11341 atttttgcca tcaacaattt tcctcataaa gtcatttcaa agcctaatag ctgctctact
    11401 tttttcctca atatcatgtc gccagcagga aacaacctaa tccctgtttt aggcctcaaa
    11461 gctgttaatg ataattattt tcattttgta taggttatgc tataaaacat agccgttgct
    11521 atgtttgttc taattttgct cactataggt actaaattat gcactcaata aaaaaagtaa
    11581 ccatgctctt ggggtgcctc gccctcacct gctcgatcgc atttcaggca agtgcaactg
    11641 aaaaattcaa ggtcattaca acatttacca tcatcgcgga tatggccaaa aacgtggctg
    11701 gagatgctgc agaagtctca tccataacca agcctggtgc agaaattcat gagtatcagc
    11761 ctacccctgg cgatattaaa cgtgcacagg gggcacaact gattctcgcc aatggtatga
    11821 atctggaatt gtggttccaa cgcttttacc agcatctcaa tggggttcca gaagtaattg
    11881 tctcttcggg tgtgacgcca gtagggatca ccgaaggacc ctatgagggc aaacctaatc
    11941 cccatgcatg gatgtcgcca gataatgctc tgatttacgt cgataatatt cgtgatgcgt
    12001 tgataaaata cgatccggca aatgcacaaa cctaccaacg taatgccgat acttataaag
    12061 ccaagattac ccaaaccctt gcccccctgc gtaagcagat tacggaactc cctgagaatc
    12121 agcgatggat ggtcaccagt gaaggggctt tttcttatct cgcacgcgat ttggggctaa
    12181 aagagcttta tctgtggccg attaatgccg atcaacaagg aacaccgcag caggtacgta
    12241 aggttgttga tatagttaag aaaaatcata tcccggcagt ctttagcgag agtacgattt
    12301 ccgataaacc agcgcgtcag gttgcgcgtg aaaccggcgc acactacggc ggtgtgctct
    12361 atgtcgattc cctgagcaca gaaaacggcc cggtacctac gtatatcgac cttcttaagg
    12421 ttactaccag tacgctggta cagggaatta aagccgggaa aagggagaaa taatgatgca
    12481 atctgcaggc attgtcgtta atgatgttac tgtcacctgg cgtaacgggc acacagcact
    12541 gcgcgatgca tccttcacag taccaagtgg atctattgcc gctctggttg gggtaaacgg
    12601 ttccggaaaa tcgacgttgt ttaaagcgat tatgggattt gtgcgtctga ccagcggaaa
    12661 gatatctgtt ctgggtattc ccacgcgaca ggcgctacag aaaaacctgg ttgcctacgt
    12721 ccctcagtca gaagaggttg actggtcatt tcctgtactg gtggaagatg tggtgatgat
    12781 gggccgctat gggtatatgg gtatgttgcg tatagccaaa aagagggatc gtcagattgt
    12841 caccgatgcg ctggaacgtg tcgatatggt ggattttcgc catcgtcaaa tcggcgagct
    12901 ttccggtgga caaaagaaac gcgtctttct ggcgagagcg attgcacagc agggtgatgt
    12961 gatcctgctg gatgaacctt ttaccggcgt tgatgtgaaa accgaagcga aaatcatcag
    13021 cctgttgagg gagttacgcg cggaaggcaa aacgatgctt gtctcaacgc acaatctcgg
    13081 ctcagtcacg acattttgcg attatacggt catggtcaaa ggcaccgtcc tggcaagcgg
    13141 gccgacagac accactttta cagccgagaa cctggagctg gcttttagcg gcgtactgcg
    13201 ccatgtcaca cttaacggct cggaagaaag cattattact gacgatgaac gcccttttgt
    13261 ggcacatcga ccgtcagcgg tgcagaggga agcaagatga acgtgctact ggaacccttc
    13321 agctatgaat atatgctcaa tgcgatgtgg gtctcggcaa tggtcggcgg tctctgcgct
    13381 ttcctgtcat gctatctgat gctaaaaggc tggtcgctga ttggcgatgc gctgtcgcac
    13441 tctattgtcc cgggtgttgc gggggcatat atgctggggc ttccgttttc tctgggagcc
    13501 ttcttttccg gcggactggc agcgggcagc atgctgtttc ttaaccaacg cacccgctta
    13561 aaagaagatg ccatcatcgg cctgatcttc tcgtcctttt ttggcctggg gctgtttatg
    13621 gtgtcattga acccgacatc cgtgaacatt cagaccatcg tcctgggcaa tattctggca
    13681 atcgatccgg cggatatcct gcaactcact atcattggca ttctctcgat catcgtgctt
    13741 tttttcaaat ggaaagatct tatggtgacc tttttcgacg agaatcacgc ccgtgctatt
    13801 ggattacatc cgggcagatt aaaaatcctc ttcttcaccc tgctgtcggt ttcgaccgtt
    13861 gcggcgctgc aaacggttgg tgcctttctg gtgatctgtc tggtggtgac tcccggtgca
    13921 acagcatggt tgttaaccga tcgttttccc cgcctgctca taattgctgt aactattggc
    13981 agtgtaacca gtttcctcgg cgcgtgggtc agttactttc tggacggcgc gacaggtgga
    14041 atcatcgtgg tggcgcagac actgctgttt cttttagcat ttgtatttgc gcccacccat
    14101 ggacttttgg caaatcgtcg gcgtgcgcat aaagcgctgg aggatcgctc atgatggcat
    14161 tattactgga gccactgcag ttcaccttta tgagtcacgc actgctgatt tcactggtag
    14221 tttccatacc ctgtgcgctg ctgtcggtct ttctggtcct gaaaggttgg gcactgatgg
    14281 gtgacgcgat gagtcatgcg gtttttcctg gcatcgtgct tacctggatt ctggggttac
    14341 ctctggctac aggggcattc gtcgccggag tattctgcgc ggtggcgacg ggatacctga
    14401 aagacaacag ccgaatcaag caagatacag ttatggggat tgttttttct ggcatgtttg
    14461 ccgcaggtct gatcttgtat attgcaatca aaccagacgt acatcttgac catattcttt
    14521 tcggcgacat gctcgggata accatcggcg atataatcca gacggtgatt attgccgggc
    14581 tggttacact tgttattagc gtaaaatggc gggatttttt gctgttcagc tttgattatc
    14641 aacaggcgca ggtaagcggt ttgcatacga gatggctgca ttatgggctg ctgtgcatgg
    14701 tttccctgac cattgtggcg acgctgaaag cagtaggtat cattctctct atttctctgc
    14761 tcattgcgcc tggcgccatt gcagtactcc tgacacaacg ctttcatatt gcgctcctac
    14821 tggcgactgg catatcggta atagtgtcaa tgactggtgt ctggctgtcc ttttttatcg
    14881 acagcgcacc agctccgacg attgtcgtct tattcgcggt cgtgttcatc atgacgttta
    14941 ccgttaccag catcaacgca cgcacaaagg gaaacgccca tacacaggat ctgttatcac
    15001 ccaattagca caatatccga ggacagcatc gtccgccagc gtccgccagc ctacagaagt
    15061 tcggaaagcc atggaaggtc gttgggagga ggtacgaatt ctatcatgca aaaaatacgt
    15121 aaaatcgata acgcctagaa atcattcaat actcgcacta tcgaaagttt accagccaac
    15181 cgcggcacgt tcctgcatac gacgtgccgc agcttctgtt attccacgat aaaattccta
    15241 accgaattca aatccttcag actattcagc tcttccttca gctcccgctg acggcgataa
    15301 at
//
LOCUS       DAJVOY010000093        15285 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVOY010000093 DAJVOY010000000
VERSION     DAJVOY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.093"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(99..524)
                     /gene="arsC"
                     /locus_tag="OQL90_002521"
     CDS             complement(99..524)
                     /gene="arsC"
                     /locus_tag="OQL90_002521"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1369258.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(537..1826)
                     /gene="arsB"
                     /locus_tag="OQL90_002522"
     CDS             complement(537..1826)
                     /gene="arsB"
                     /locus_tag="OQL90_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1369259.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="OQL90_002523"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="OQL90_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1369260.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            2973..3056
                     /locus_tag="OQL90_002524"
     CDS             2973..3056
                     /locus_tag="OQL90_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1369261.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3110..4462)
                     /gene="gorA"
                     /locus_tag="OQL90_002525"
     CDS             complement(3110..4462)
                     /gene="gorA"
                     /locus_tag="OQL90_002525"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1369262.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4534..5376)
                     /gene="rlmJ"
                     /locus_tag="OQL90_002526"
     CDS             complement(4534..5376)
                     /gene="rlmJ"
                     /locus_tag="OQL90_002526"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1369263.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            5579..7621
                     /gene="prlC"
                     /locus_tag="OQL90_002527"
     CDS             5579..7621
                     /gene="prlC"
                     /locus_tag="OQL90_002527"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1369264.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7629..8381
                     /gene="rsmJ"
                     /locus_tag="OQL90_002528"
     CDS             7629..8381
                     /gene="rsmJ"
                     /locus_tag="OQL90_002528"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1369265.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(8430..9899)
                     /gene="dtpB"
                     /locus_tag="OQL90_002529"
     CDS             complement(8430..9899)
                     /gene="dtpB"
                     /locus_tag="OQL90_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCS1369266.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10217..10651)
                     /gene="uspA"
                     /locus_tag="OQL90_002530"
     CDS             complement(10217..10651)
                     /gene="uspA"
                     /locus_tag="OQL90_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1369267.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11042..11377
                     /gene="uspB"
                     /locus_tag="OQL90_002531"
     CDS             11042..11377
                     /gene="uspB"
                     /locus_tag="OQL90_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1369268.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11621..13120)
                     /gene="pitA"
                     /locus_tag="OQL90_002532"
     CDS             complement(11621..13120)
                     /gene="pitA"
                     /locus_tag="OQL90_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1369269.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13352..14554
                     /locus_tag="OQL90_002533"
     CDS             13352..14554
                     /locus_tag="OQL90_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1369270.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(14869..>15285)
                     /locus_tag="OQL90_002534"
     CDS             complement(14869..>15285)
                     /locus_tag="OQL90_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HCS1369271.1"
                     /translation="QTRNTFSTKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAP
                     GIILIYLGMICYSIFSKVWLLALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQ
                     TDMGYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKK"
BASE COUNT         3858 a         3911 c         4015 g         3501 t
ORIGIN      
        1 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
       61 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
      121 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
      181 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
      241 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
      301 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
      361 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
      421 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
      481 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
      541 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
      601 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
      661 acgtgcagcc agagtagtgt agccaggcta ccaattgggg taattttcgg tcccaaatcg
      721 cagccaatca cattggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
      781 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
      841 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
      901 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
      961 atgccgagcg agaagatgac aatttgccag ggggcaccgc gcaggacttt acccgtatta
     1021 atcgcatgac cgcgtttagc gacgacaaat aatatcagcg cgcccacagc tgcaatggcg
     1081 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     1141 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     1201 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     1261 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     1321 ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg
     1381 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     1441 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgctgcgcc atcattggca
     1501 aacagggcgg caacggcagc accgagcagg acaatccagg taaacagcaa gcgaccacga
     1561 ccattacccc agcgtgagac gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc
     1621 agcaggctga tgataatgac ggcgataaac gcagccgtcg cgttccagac gatattccac
     1681 accaccggaa tatcacccgg atggaccacg cccgtaacta acgctagtac tgcgccgagc
     1741 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac aatggtcagg
     1801 acaaagatag cgcctgccag taacataatg cctcctggat aaaacacatg cgaaaataca
     1861 tatgtgttta gctaaatttt ttaactgcaa atgttcttac tgtctgcgga acagttttgt
     1921 cgagccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 attattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccatttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccaggtggcg ggagatcttg
     2101 ggctgcgact ggtcgagagc agtgcagaga tcgcagacgc ataactctcc cagttcgctg
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgat ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggag gtctgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactttc cagccctgag ttggtggctc tggctggagt gagagagttc
     2461 ttatctaaca gctcaatact attagccagt ggctaacatt gcaagaatta gcgatttctt
     2521 cagctggcgg agggagcact tcggtatata tcgcagtgac gatttatata ctttcactgg
     2581 gtcatcgtca tattaagcct tgtccgaatc cggatgtaaa aaaccgactt tgcgtcggtt
     2641 ttttactttc cagccctgag ttggtggctc tgactggagt gagatagcca tcatctaacg
     2701 catcaatgct aataccatac tgaaactatt gcaaggacgt gctggtttta taacctgcat
     2761 gtactgtatg attatccagt tagctctgag gcattttcac tctggcaatg cgcataaacg
     2821 ctttcaaagt cctggtcaga agtacgggtg gtgccgttaa ctgatgctct ggccggagtg
     2881 agagagttct tatctaacaa tgagacatgc gccgtgacag gcagtggatg agtaagcgga
     2941 tgcattctca ctccatcgca tggagaaaac gggtgattga taaagcaatc atcgttctag
     3001 gggcgttaat tgcgctgctg gaactgatcc gctttctgct tcagcttctg aactgatagc
     3061 ggaaacgtaa ttaagggcta agagcacact actcttagcc ctttaacatt taacgcattg
     3121 tcacgaactc ttctgccgcc gttgggtgaa tggcgacggt attgtcgaag tcttttttgg
     3181 ttgcccccat cttcagcgcc accgcgaagc cctgcaacat ttcgtccata ccaaagccaa
     3241 tgccgtgaat accgacaatc ttctcttccg atccaacgca caccagcttc atgcggcacg
     3301 gctggcggtg agtggtgacg gcggtataca tcgcggtgaa agaggattta tacactttca
     3361 cctgatcgtc gccatactgc tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg
     3421 gcggatggct gaagaccacg gtcggaatgt tgctgtaatc cagatgctca tccggcttgt
     3481 tattaaacag gtgttcagag agacggcgac ccgctgcaac tgccaccggt gtcagctcca
     3541 ctgcacccgt gttatcgccc accgcgtaaa taccttcaat gttggtgttt tgatatttat
     3601 cgacgacgat atagcctttt tcgttggttt taacgccagc ggcttcgagg ttgatgttgt
     3661 cattggcagg ctcgcgacca atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat
     3721 cttccagctc cagcgtcagg ctaccatcgg catttttcac taccgctttc gggatggcgt
     3781 tggtgtgcag ctgcgggcct tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg
     3841 ggtcgaagct gcgcagcggc gcatgtttac gcacaaacag atgcgttttc gcgccgaggc
     3901 cgttaatcac gcccgccagc tcaacggcga tgtaacccgc gccaacaacc gccacgcgct
     3961 ctggcaaagc aggaagggcg aagaagccat cagaatcaat accgtattcc acgcccggaa
     4021 tatccgggtg gctcggacga ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt
     4081 cgccgtttac ctccagcatt ttggcatcaa cgaagcgggc aaagcctttg attacatcaa
     4141 cgttattttt accgagcacg ttttcatagg aagtatgaat acggtcgata taggcggtac
     4201 ggctggcgat caacgtttcc cagttgaact tgttgatggt ggtatcgaaa ccataatcag
     4261 ggccgtacat atggatcgct tcacggattt gcgccgcgtg ccacatcact tttttcggca
     4321 cgcagccgac gttcacgcag gtgccgccca gctctttggc ttcaatcagt gcgcatttct
     4381 ggccgtacat agccgcgcgg ttgatggagg ctataccgcc gctgccgccg ccgatggcga
     4441 tgtaatcata gtgtttagtc atgacagagt gtccttatcg ttgattaccg cgattgtagc
     4501 gcgagacgta ataggtgcca gcaatggctg caattactcc ggcacgatcc agcttgcggt
     4561 ggcgtgcccg gtgcctgccg gaaccagttt gctgtgcagc cacggcagca cgttattcat
     4621 ctgctgttcc agtttccacg gcgggttaat cacaatcatg ccggaagcgg tcatgccacg
     4681 gcgatcgctg tctggcagta ccgccagttc aatttgcaga attttgcgaa taccggtcgc
     4741 ttccagatcg tggatcatgc gcttaatttg ctgacgcagc accaccggat accacagtgc
     4801 gtaagtacca gtggcgaaac gtttgtaacc ttctgctatc ccgctgacca ccgcctgata
     4861 gtcggttttc atttcatacg gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg
     4921 cagtttggcc ttaagctgct ggaaaccatc ggctttttcg acgcgcgcac ggctgtcttt
     4981 ctgaaattca gaacgcagca acgggtaatc gctcgggtgc agttcggtca gttgcaggct
     5041 gtcttgttcg cgcagtagct ggcgagcaat caatggcgaa ccggggtagt aacgcaactg
     5101 accgctacgg ttgaagtgtt ttaccacatt gatgtacgcc tccagttctg cgggcaaatc
     5161 gtcctgctgc cagatacggg cgatgccttc gagatattcg ccggtacgct cggcatgttc
     5221 gctgcctaac tgataacgcc cggcccctgc gtgggtgtcg agatagagaa acggtttatc
     5281 tttctctttc agcgactcga tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg
     5341 gttgccagcg tgaaagctgt ggcgataact gagcatgggt aaaggtgttc cggtaagtaa
     5401 aaatcggccc gtattcgggg cgcacaaaag cgtatcagga cagtataccg aaaagaggcc
     5461 gccgccgcga aagcgtaacg tttctcattg aaattcacta cacttaaccc catgctacac
     5521 acattatgta aagcgcctgt tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat
     5581 gacgaatccg ttactgactc cctttgaatt gcctccgttt tctaaaattc tcccggaaca
     5641 tgtcgttcca gccgtgactc aggcgctgaa cgattgccgc gaaaatgtgg agcgcgtagt
     5701 agcgcaaggg gcaccgtaca cctgggaaaa tctctgccag ccgttggcgg aagtggacga
     5761 tgtgttgggg cgtatcttct ccccggtcag ccacctgaac tcggtgaaaa atagcccgga
     5821 actgcgtgaa gcctacgagc aaaccctgcc gctgctgtcg gaatacagca cctgggtagg
     5881 gcaacatgaa gggctgtata aagcgtaccg cgacctgcgc gatggcgatc attacgccac
     5941 gctgaacacg gcgcagaaga aagcggttga taacgccctg cgtgattttg aactctctgg
     6001 cataggtctg ccgaaagaga aacagcagcg ttacggcgaa attgcgaccc gtctttctga
     6061 actgggcaac cagtacagca acaacgtcct cgatgcgaca atgggctgga ccaaactcgt
     6121 taccgacgaa gcggagctgg cggggatgcc tgaaagcgcg ctggctgcgg caaaagccca
     6181 ggccgaagcg aaagagctgg aaggctacct gctgacgctg gatatcccaa gctacctgcc
     6241 ggtaatgacc tactgcgaca accaggcctt gcgtgaagag atgtatcgcg cttacagcac
     6301 ccgcgcctcc gatcaaggcc cgaacgccgg taaatgggat aacagcaagg tgatggaaga
     6361 gatcctcgct ctgcgtcacg aactggcgca actgctgggc tttgaaaact acgcctttaa
     6421 atcccttgcc actaaaatgg cagaaaaccc gcagcaggtg ctggatttct taaccgatct
     6481 ggcaaaacgc gcgcgtccgc aaggcgaaaa agagctggca caactgcgcg ccttcgccaa
     6541 agcggaattt ggcgtcgatg agttgcagcc gtgggatatc gcgtactaca gcgaaaaaca
     6601 aaaacagcac ctctacagca tcagtgacga acagctgcgt ccgtacttcc cggaaaacaa
     6661 agcggttaac ggcctgtttg aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg
     6721 taaagatgtt gatgtctggc atccggatgt acgtttcttc gaactgtatg acgagaacaa
     6781 cgaactgcgc ggcagcttct acctcgatct gtatgcccgt gaaaacaagc gcggcggggc
     6841 gtggatggat gactgcgtag gccagatgcg taaagctgac ggttctctgc aaaaaccggt
     6901 cgcgtatttg acttgtaact tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca
     6961 cgacgaagtg atcaccctgt tccacgagtt cggtcacggc ctgcaccata tgctgacccg
     7021 catcgaaacc gctggtgttt ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc
     7081 gagtcagttt atggaaaact ggtgctggga gccggaggcg ctggcgttta tctctggtca
     7141 ctatgaaacc ggcgaaccgc tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa
     7201 ctaccaggcg gcgctgttta ttctgcgcca gctggagttc ggcctgttcg atttccgcct
     7261 ccatgccgag ttccgcccgg atcagggtgc gaaaatcctc gaaactctgg cagaaatcaa
     7321 gaaactggtt gccgtagtac cgtctccatc ctggggccgt ttcccgcacg ctttcagcca
     7381 tattttcgcc ggtggttatg ccgcaggtta ctacagctac ctgtgggctg acgtactggc
     7441 ggcagatgcc ttctcgcgct ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc
     7501 gttcctcgac aacattctga gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg
     7561 cttccgtggt cgtgaaccgc agctggatgc gatgctggag cattacggca ttaagggctg
     7621 atcattcagt gaaaatctgc ttaattgatg aaacaggcgc cggagacggc gccttatctg
     7681 ttctggcggc ccgctggggg ctggagcacg atgaagacaa cctgatggcg ctggtgttaa
     7741 cgccggaaca tctggaattg cgcaagcgtg atgagccaaa acttggcggc atctttgttg
     7801 attttgttgg cggagcgatg gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg
     7861 tggcgaaagc ggtgggcatt aaaggcgatt atttgccgga tgtggtggac gccaccgcag
     7921 ggctggggcg cgatgccttt gtactggctt cagtcggctg ccgcgtgcgg atgctggagc
     7981 gtaatccagt ggttgccgcg ctactcgacg acggcctggc gcgtggttat gcggatgcgg
     8041 aaatcggcgg ctggttgcag gagcggttgc agttaattca cgcctccagc ctgacggcgc
     8101 taaccgatat taccccacgc ccgcaggtgg tctatctcga tccgatgttc ccgcataagc
     8161 agaaaagcgc gctagtgaaa aaagagatgc gtgtctttca gtcgctggtg ggaccggatc
     8221 ttgatgccga tggattactg gagcctgcac gcttgctggc gaccaaacgc gtggtggtaa
     8281 agcgcccgga ctacgcgcca ccgctagcga atgtcaccac gccaaacgcg gttgtgacta
     8341 aagggcatcg ctttgatatc tatgcaggta cgcccgtgta aaagtaaagc agggtagcga
     8401 taacgctacc ctgctttctg caagaataat taatggcttt ccggcgtcgc aatcatgcgt
     8461 ttcagccacg gcaccatcaa cagcatcact actgcaacgc ccagcgtgac cagaccaatc
     8521 ttaccaaaca cgttggtata gacgggcaac gtctcaagcg gatcggtaat gttgtccggc
     8581 accgcggtaa atgttgccac atagccgccc agcaagaacg cggcagcctg cgtcaggaac
     8641 cacatcccga gaataaagcc catcaaatgc tgcggcacca gggcagcaat catcgccagg
     8701 ccaagggcgc taataaacag ttcacctaag ctctggaata agtacaccag cacgataaac
     8761 catggcgatg tcagcccttg tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa
     8821 aagcccagtg agcacataaa catgccgaga gtaaatttca tcggcatcga gaggtctttg
     8881 cctttgttac ccagatgcgt gtaaatgcct gccagtattg ggctggcgag taccacccag
     8941 aacgggttaa gcgcctggaa gctgaccgag ttgatggaaa aaccgagaat ttcatgatgc
     9001 acgttgttga tggcaaagaa gttcagcgat gttggcatct gggcgtagag aatgtaaaac
     9061 accaccgctt cgagcatcag gacaaaggcg acaaacattt tattgcgccc ggttttatcc
     9121 agcttgaatg cctgacgaaa gaagatgatg gtgacgacga tggagagaac aatcagcacc
     9181 agattggcga cttctacgtt gtgcatcagc catgcacata cgaagatcat caccacgctg
     9241 ccaagtaata cgtacaacag tttgctgaag ctcatcggct tgaagtcggg ttcagaacca
     9301 atgtctttca ccattccacg acaggcgatg taaaccagta atgcgataat taaccccgcc
     9361 ccgcacaggt tgtaggtgac tgaataaccg aatctatcag cgatcacagg ggccagcgat
     9421 aacgctatca acgagccgat attgatcgac atatagaaca gggtgaatgc gccatcaagc
     9481 cgcggatctt tcggcggata gcacttcgaa agcaagctgg ctgggttagc tttaaacagg
     9541 ccgttaccga cagcgatagt ccccagggcg atgaaaatca ggtcaggctt aagtagcgac
     9601 atgccggtca tgaagtagcc aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg
     9661 gtccccagca ggtggtcgcc gacatagccg ccaatggaaa tgaggccata gaccagcgca
     9721 gcaaaagcac caaaagtgac aaaagcctgc tcttgcgaga atccaagctg tttaacgaag
     9781 aaaaccgcca gtacgccctg cacgccgtag tagccgaatc gctcccataa ctcgacaaaa
     9841 aagatcatga aaaatgggcg aggttgctgc agcatcccca tgggtgttgt tgtattcata
     9901 tttattaacc ttccaatacc atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa
     9961 aagtttacat ctgtcagaga ttcttaatat tgatgcggtg aatggattaa tacactatta
    10021 caacagaaaa taaccagcta atcacactaa tgatggaaaa tatgatggtg ttaaatgttg
    10081 tattggcagg agattttgat tgcgtacgca gatcgtaggc cagataaggg gtttacgctg
    10141 catcaggtaa caaggcgcgc caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca
    10201 cgtcgtggag ggaagattat tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt
    10261 gaacggtgtt gatcagctga cgtgcggaag acatcagttt gctccagaag tcctggtggt
    10321 gaccacaaac caccagatcc atatcgtatt tcttgattgc atcgaccaga acctggccca
    10381 ggtcgccgct gccgctcagg gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg
    10441 tcagcgcatg atgtgtctct tcagagatgc gtttctgcat atcacccaga ttcacatcaa
    10501 taagcccggt gtataggtca gagtagttta catctacgtg gatcagagaa actttcgcat
    10561 tgtaggggcg agccatagag actgctttct ctaccagaac tttgctttcc ggggagagat
    10621 cgaccgcgat gagaatgtgt ttataagcca tagtgttact ccttccataa agttgtcgat
    10681 gactggccag ctagcgtttc ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg
    10741 cggggctaac ccagccgtaa agattcagtg ttaagaccat ccttacctta tagcgacccg
    10801 gatgatccgt caatccgcct tgcttacccg ttagataaac aaattttcga catgaatata
    10861 ttgatagtgg ttaaccttct ggaaaaaaaa caacctgatc tcctacacta tctatagagc
    10921 cgctcggatg ttggtcacca caaaagcggt ttctccagga cctggtgtta actgattggc
    10981 agcgtatcgg gagcggtagc cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg
    11041 tatgataagc accgtcgcat tattttgggc tttatgtgtc gtttgcattg ttaacatggc
    11101 gcgctatttc tcatcactac gcgcgttgtt agtggtactg cgtaactgcg atccattgct
    11161 ctatcaatat gttgatggag ggggcttttt tacctcacat ggccaaccca acaaacaggt
    11221 gcgtctcgtt tggtatatct atgcccaacg ttatcgcgat catcacgatg atgagtttat
    11281 tcgccgctgt gagcgggtgc gtcggcagtt tattctgact agcgcattgt gtggtctggt
    11341 ggtggtcagc ctgattgcat tgatgatttg gcattaagca cttcagcaat aataacgcat
    11401 aaaaaaagct ggccagtaaa ctgacccgct cgagactgat tacaaacgca aaattgcctg
    11461 atgcgctacg cttatcaggc ctgcataatc tctgcaatct attgaatttg catgtttttg
    11521 taggccgaat aaggcgttca cgccgcatca ggcatgaaca aagcgcacta tgtcacaatc
    11581 tgaagcgggc cagctgaccc gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga
    11641 gccagtacag cccgccggaa agcagtaccg cagcaggaag ggtaaacacc caggccatca
    11701 gaatgctggt cacggtttta cgctgtaagc cgccaccatc taccaccatc gtccccgcga
    11761 cagaagagga gagtacgtga gtagtggaaa ccggcatccc ggtataactc gccaggccga
    11821 tagacactgc cgccgtcatc tgggcagaca tcccctgagc gtaggtcatg cctttcttac
    11881 cgattttctc accgatagtc gttgccacac gacgccagcc aatcatcgta ccgataccta
    11941 acgccagcgc gaccgccatg atgatccaca ccggtgcata ctcgatggtg ctaagcatgt
    12001 cggacttcag tttcttcaac aggcgctgat cgtcagcact cacgccaggt atcttcacta
    12061 ctttgtcgat agtgtcagaa acgcacagca taatgcggcg catctggcta cgttgatcaa
    12121 gcgacagctt gtcgtagctt tccacatcgg tagttaacat acctttcagg cggttgagcg
    12181 cgttaatggt attcgacgga tggcaatgga actccgcggg ttgcgttgcg ccagcttccg
    12241 gagccggtac taactgatca gcaccggtag cctgtttcag cagcgcagga tgctgctcaa
    12301 agtaagcttc gacgttgttg atggcatcac gggtacgggt gatttcgtag ccagtggcat
    12361 tcatattcac cacgaagcct gctggtgcga caccaatcaa taccaacata accagaccaa
    12421 tgcctttctg accatcgttc gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca
    12481 gcgcaatacg cgtccagaac ggcggctttt tcttgccgtc tttcttttca cgctccgctg
    12541 gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa
    12601 tcagaccgcc agcaaacacc aggccgacaa taggggaaac aatcagagaa ccgaaaatac
    12661 ttaatacttt cgggatattg agtgcatcca ccactgacgt cccggtcatc aacgcattgg
    12721 ttaaaccaat cccgatgatc gcgccaatca gtgtatgaga gctggatgca ggtaaaccaa
    12781 agtaccaggt acccaggttc cagataatcg ccgccagcaa catagagaac accatggcaa
    12841 ggccatgaga cgatcccata ttaagcagca gatccgtcgg cagcatatgc acaatggcat
    12901 aggcaacact cagaccaccc agcaaaacac ccaaaaagtt gaacaccgcc gccataacca
    12961 cggcgagctg agaacgcatc gcgcgggtat agataacggt tgccacggcg ttggctgtgt
    13021 catggaaacc attgatggct tcgtagaaca gcacaaaagc cagtgcaagc aataataaca
    13081 gcccggtatg caaatccagg ccagcaaaca aatgtagcat aggacgttac gccattttga
    13141 ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc gggcaaagtg aaatatagac
    13201 tttttttgat ttgcctcctg tatggatttc actcaaaaaa taattatctt atataattca
    13261 ggcaaatact tccttttagt aatattgatg ctggtgtgac cactgaggaa tctttacaat
    13321 tcacgctcgt tttttctaag aggagcgcaa cgtggaaagg tttgatgcca ttattatagg
    13381 cgctggtgcg gcgggtatgt tctgttctgc gctggcaggt caggcaggac gccgggttct
    13441 gctgatcgat aatggtaaaa aaccagggcg caaaatcctt atgtctggcg gtgggcgctg
    13501 caactttacc aacctttatg tcgaaccagg cgcttatctg agccagaatc cgcatttttg
    13561 taagtctgca ctcgcgcgtt ttacccagtg ggatttcatt gatctggtca ataaacacgg
    13621 catcgcctgg cacgagaaaa cgttaggaca actcttctgc gatgactccg cgcagcagat
    13681 tgtcgacatg ctggtggatg agtgcgagaa gggcaatgta accttcagat tgcgtagcga
    13741 agtgctgagt gtggcgaagg atgaaacagg cttcacgctt gaactgaacg gcatgactgt
    13801 cggttgcgaa aagctggtca tcgcgaccgg tgggctgtca atgccggggc tgggcgcatc
    13861 gccgtttggt tataagattg ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg
    13921 tctggtgcca ttcactctgc ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt
    13981 ggcggtgcct tccgtgatta ccgctgaaaa cggcaccgtt ttccgtgaga acttactctt
    14041 cacccaccgc ggcttatctg gaccggcggt gttgcagatt tccagctact ggcaaccggg
    14101 tgaatttgtc agtatcaatc tgctaccgga tgtggacctc gaaaccttcc tgaatgagca
    14161 gcgtaacgca catccgaatc agagcctgaa aaacacactg gcggttcatc taccgaagcg
    14221 gttggttgaa cgcttacagc aactcgggca aatcccggat gtttcgctaa aacagctcaa
    14281 cgtgcgtgac caacaggcac tgattagcac attgaccgac tggcgcgtac aacccaacgg
    14341 cactgaaggc tatcgcactg ccgaagtgac gctcggcggc gtggacacca acgaactctc
    14401 ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga
    14461 cgttaccggc tggctggggg gctataactt ccagtgggcg tggtcgagtg cgtgggcttg
    14521 tgcgcaggat ttgattgcag cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt
    14581 agagccgcat taaaaatgag ctaattttga tagtggctat cttgtgatta ttttctaatg
    14641 agcccgtgaa ctgaaaccct ccaggcttaa tataaggtgg aaggaaagat gattgaaaac
    14701 tcacccagtg gccagatctt tataaaatat gaacaggctg aaaaagacag taactttgtt
    14761 gttttttatc cttaaccact atgcattaat gctgcgttat ttcatgatgc ctaagaaaaa
    14821 ccagcgttac gcaaatggtc aacgctggtt ttatccggta cgttgcaatt attttttagc
    14881 agaacctgct tctaatattg aaacgattga gaacaacgta aagcataccg ctcccaaacc
    14941 gaccaaaacc cgcgaaggaa taaaatatcc catgtcggtc tgcgccattt ccgcaagaaa
    15001 cgatgccagg aaaaggcaaa acaggcaggt gaagacggga atcatcggta ttctgttggc
    15061 taacgaacag gtacgtctcc ataccagtgc cagtagccag acttttgaga atatgctgta
    15121 acagatcatt ccgaggtaaa taagaataat gccgggagcc agtcgggcgc ttgcatcgga
    15181 actgactaag acgtatatcc cctgcagtac cgtgattgag ccgagaaaaa taacccaata
    15241 acaccacagc cagtgttctt tagttgaaaa cgtattacgt gtttg
//
LOCUS       DAJVOY010000094        14936 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVOY010000094 DAJVOY010000000
VERSION     DAJVOY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.094"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..450
                     /locus_tag="OQL90_002535"
     CDS             <1..450
                     /locus_tag="OQL90_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCS1369272.1"
                     /translation="QEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIA
                     DPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMT
                     LGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(491..1423)
                     /locus_tag="OQL90_002536"
     CDS             complement(491..1423)
                     /locus_tag="OQL90_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1369273.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            1787..4573
                     /gene="polA"
                     /locus_tag="OQL90_002537"
     CDS             1787..4573
                     /gene="polA"
                     /locus_tag="OQL90_002537"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1369274.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4955..5587)
                     /locus_tag="OQL90_002538"
     CDS             complement(4955..5587)
                     /locus_tag="OQL90_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1369275.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6169..6678
                     /gene="yihI"
                     /locus_tag="OQL90_002539"
     CDS             6169..6678
                     /gene="yihI"
                     /locus_tag="OQL90_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1369276.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6867..8240
                     /gene="hemN"
                     /locus_tag="OQL90_002540"
     CDS             6867..8240
                     /gene="hemN"
                     /locus_tag="OQL90_002540"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1369277.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8430..8540)
                     /locus_tag="OQL90_002541"
     CDS             complement(8430..8540)
                     /locus_tag="OQL90_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1369278.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8651..10060)
                     /gene="glnG"
                     /locus_tag="OQL90_002542"
     CDS             complement(8651..10060)
                     /gene="glnG"
                     /locus_tag="OQL90_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1369279.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10072..11121)
                     /gene="glnL"
                     /locus_tag="OQL90_002543"
     CDS             complement(10072..11121)
                     /gene="glnL"
                     /locus_tag="OQL90_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1369280.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11295..12704)
                     /gene="glnA"
                     /locus_tag="OQL90_002544"
     CDS             complement(11295..12704)
                     /gene="glnA"
                     /locus_tag="OQL90_002544"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1369281.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13077..14900
                     /gene="typA"
                     /locus_tag="OQL90_002545"
     CDS             13077..14900
                     /gene="typA"
                     /locus_tag="OQL90_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1369282.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3864 a         3744 c         3852 g         3476 t
ORIGIN      
        1 caagaagtga atgctgcatt gttcaaagag tatttaccgt tattacaaca aagtgagccg
       61 accattaaac aaccggtaag atggaagaac gcactcggcg aactaaatgc caatctggat
      121 atcagtattg ccgacccagc caaatcttca tcatccacaa acaaagatat caaatcgctc
      181 aattttgatg tgaagttacc gcttaatgtc gtcacagaaa ccgcaaaaca gcttaattta
      241 tctgaaggaa tggatgcgga aaaagcgcaa aagcgggctg ataaacaaat cagcgggatg
      301 atgaccttag gtcagatgtt tcagttaatc acgattgaca acaataccgc ctcgctgcaa
      361 ctgcgttata caccgggtaa agttgttttt aacggacagg agatgagcga agaagaattt
      421 atgtctcgtg ccggacgttt tgttcattaa aatactcact cgcctctgct cattcagagg
      481 cgagcaaaat ttacttgtcc tgccgctgtg atgacatcaa cgacgtcaga agtcggtctt
      541 tttcctgcca cagtgagttc agccattgct gaaaatggcg cttaaagctt ttgtcattga
      601 tgtagtcgcc atgtaactca tcggcaatag gctgtaaatc cacatgaacg acaattcgcg
      661 tcagtttacc gcttaacata tcgaagaacg gctgacggtt attgtccgga taacacagcg
      721 taacattaag cagtttatcg aattgtttac ccagtacatt tagcgccatc gcaatgcctg
      781 ccgcctttgg tggcaacaag ttttgaaaag tggagtgggt ttgctgatgt ttttcttgcg
      841 tgaaacggga gccttcaacg aaattgacaa tggtggtggg atgcaggcga aacttttcac
      901 aagaacgccg agtggtttca acatctttac cacgtcgctc cggatgacgt aataaataag
      961 cgcgggaata acgcttcata aacggcatat ccagcgccca gcacgccagg ccaagaaatg
     1021 gcacccaggc cagctgttgc ttgaggaaat atttattcat cggaatgtgc ttacgaaaca
     1081 gcacgcacag tacgacaata tctgcccaac tacggtggtt acaaataagc agataccagt
     1141 tcttcttact taacccttcc agcccgtgaa cttcccattg caggtgtggg ttcagatgca
     1201 gtaataccgc cagaccttca caccagcaat acatcataaa atcacaaaaa cgcgaaacct
     1261 ttcgccaaat gactggcaca ggtagcaata gttttacaat cccggcaatg atgatcggga
     1321 cggaacaaaa aatagtgacc aaaatggtca atacgatact cagcaaaagg gttatcgcag
     1381 cgagtattct cgtcataatg aatttattca aaaggttagc catagacagt gcgctaaaga
     1441 agcgcaaggc gcagatttta gcagaaaacg accgaaataa cggatgatcc ttaaggagaa
     1501 aaataattca tatctatcca cattagaaaa aatcccatta tctcaattat tagggatggc
     1561 tttattttta actacatgaa aaacaaagac aaacatcata ctgtaaaaag catgataata
     1621 aattaaaagc gatgtaaata atttatgcac aaagttatcc acatgacgat ttgcgagcga
     1681 tccagaagat ctacaaaaga ttttcacgaa aagcggtgaa aaactcatgt ttttatcctg
     1741 tctgtggcat cctttaccca taatctgata aacaggcacg gacattatgg ttcagatccc
     1801 ccaaaatcca cttatccttg tagatggttc atcttatctt tatcgcgcat atcacgcgtt
     1861 tcccccgctg actaacagcg caggcgagcc gaccggtgcg atgtatggtg tcctcaacat
     1921 gctgcgcagt ctgatcatgc aatataaacc gacgcatgca gcggtggtct ttgacgccaa
     1981 gggaaaaacc tttcgtgatg aactgtttga acattacaaa tcacatcgcc cgccaatgcc
     2041 ggacgatctg cgagcacaaa tcgaacctct gcacgcgatg gttaaagcga tgggactgcc
     2101 gctgctggcg gtttctggcg tagaagcgga cgacgttatc ggtactctgg cgcgcgaagc
     2161 cgaaaaagcc gggcgtccgg tgctgatcag cactggcgat aaagatatgg cgcagctggt
     2221 gactccaaat attacgctta tcaataccat gacgaatacc atcctcggac cggaagaggt
     2281 ggtgaataag tacggcgtgc cgccagaact gatcatcgat ttcctggcgc tgatgggtga
     2341 ctcctccgat aacatccctg gcgtaccggg cgtcggtgaa aaaaccgcgc aggcattgct
     2401 gcaaggtctg ggcgggctgg atacgctgta tgccgagcca gaaaaaattg ctgggttgag
     2461 cttccgtggc gcgaaaacaa tggcagcgaa gctcgagcaa aacaaagaag ttgcttatct
     2521 ctcataccag ctggcgacga ttaaaaccga cgttgaactg gagctgacct gtgaacaact
     2581 ggaagtgcag caaccggcag cggaagagtt gttggggctg ttcaaaaagt atgagttcaa
     2641 acgctggact gctgatgtcg aagcgggcaa atggttacag gccaaagggg caaaaccagc
     2701 cgcgaagccg caggaaacca gtgttgcaga cgaagcgcca gaagtgacgg caacggtgat
     2761 ttcttatgac aactacgtca ccatccttga tgaagaaaca ctgaaagcgt ggattgcgaa
     2821 gctggaaaaa gcgccggtat ttgcatttga taccgaaacc gacagccttg ataacatctc
     2881 tgctaacctg gtcgggcttt cttttgctat cgagccaggc gtagcggcat atattccggt
     2941 tgctcatgat tatcttgatg cgcccgatca aatctctcgc gagcgtgcac tcgagttgct
     3001 aaaaccgctg ctggaagatg aaaaggcgct gaaggtcggg caaaacctga aatacgatcg
     3061 cggtattctg gcgaattacg gcattgagct gcgtgggatt gcgtttgata ccatgctgga
     3121 gtcctacatt ctcaatagcg ttgccgggcg tcacgatatg gacagcctcg cggaacgttg
     3181 gttgaagcac aaaaccatca cttttgaaga gattgcgggt aaaggcaaaa atccactgac
     3241 ctttaaccag attgccctcg aagaggccgg acgttacgcc gccgaagatg ctgatgtcac
     3301 cttgcagttg catctgaaaa tgtggccgga cctgcaaaaa cacaaagggc cgttgaacgt
     3361 cttcgagaat atcgaaatgc cgctggtgcc ggtgctttca cgcattgaac gtaacggtgt
     3421 gaagatcgat ccgaaagtgc tgcacaatca ttctgaagag ctcaccttgc gtctggctga
     3481 gctggaaaag aaagcgcatg aaattgcagg tgaagaattt aacctttctt ccaccaagca
     3541 gttgcaaacc attctgtttg aaaaacaggg tattaaaccg ctgaagaaaa cgccgggtgg
     3601 cgcgccgtca acgtcggaag aggtactgga agaactggcg ctggactatc cgttgccaaa
     3661 agtgattctg gagtatcgtg gtctggcgaa gctgaaatcg acctacaccg acaagctgcc
     3721 gctgatgatc aacccgaaaa ccgggcgtgt gcatacctct tatcaccagg cagtaactgc
     3781 aacgggacgt ttatcgtcaa ccgatcctaa cctgcaaaac attccggtgc gtaacgaaga
     3841 aggtcgtcgt atccgccagg cgtttattgc gccagaggat tatgtgattg tctcagcgga
     3901 ctactcgcag attgaactgc gcattatggc gcatctttcg cgtgacaaag gcttgctgac
     3961 cgcattcgcg gaaggaaaag atatccaccg ggcaacggcg gcagaagtgt ttggtttgcc
     4021 actggaaaca gtcaccagcg agcaacgccg tagcgcgaaa gcgatcaact ttggtctgat
     4081 ttatggcatg agtgctttcg gtctggcgcg gcaattgaac attccacgta aagaagcgca
     4141 gaagtacatg gacctttact tcgaacgcta ccctggcgtg ctggagtata tggaacgcac
     4201 ccgtgctcag gcgaaagagc agggctacgt tgaaacgctg gacggacgcc gtctgtatct
     4261 gccggatatc aaatccagca atggtgctcg tcgtgcagcg gctgaacgtg cagccattaa
     4321 cgcgccaatg cagggaaccg ccgccgacat tatcaaacgg gcgatgattg ccgttgatgc
     4381 gtggttacag gctgagcaac cgcgtgtacg tatgatcatg caggtacacg atgaactggt
     4441 atttgaagtt cataaagatg atgttgatgc cgtcgcgaag cagattcatc aactgatgga
     4501 aaactgtacc cgtctggatg tgccgttgct ggtggaagtg gggagtggcg aaaactggga
     4561 tcaggcgcac taagattcgc ctgaacatgc ccttttttcg taagtaagca acataagctg
     4621 tcacgttttg tgatggctat tagaaattcc tatgcaacaa ctgaaaaaaa attacaaaaa
     4681 gtgctttctg aactgaacaa aaaagagtaa agttagtcgc gtagggtaca gaggtaagat
     4741 gttctatctt tcagaccttt tacttcacgt aatcggattt ggctgaatat tttagccgcc
     4801 ccagtcagta atgactgggg cgttttttat tgggcgaaag aaaagatccg taatgcctga
     4861 tgcgctatgt ttatcaggcc aacggtagaa ttgtaatcta ttgaatttac gggccggata
     4921 cgccacatcc ggcacaagca ttaaggcaag aaaattattc gccgtcctgc gtttcttcta
     4981 caggctgcat ctcgctaaac caggtatcca gtttctgccg cagcttgtcc acgccttgtt
     5041 tcttcaacga agaaaacgtt tcaacctgca catcaccgtt aaacgccagt acagcttcac
     5101 gcaccatatt caattgcgct ttacgtgcgc cgcttgccag tttgtccgct ttggtcagca
     5161 gcaccagaac ggcgatattg ctgtctaccg cccactcaat catctgctga tccaaatctt
     5221 tcagcggatg gcgaatatcc attagcacca ccagaccttg caggctatga cgtttttcga
     5281 ggtattcgcc gagcgcacgc tgccatttgc gcttcatctc ttccgggact tccgcataac
     5341 cgtacccagg caagtcaacc agacgcttgc cgtcagccac ttcaaacagg ttgataagct
     5401 gggtgcgccc tggggttttt gaggtacgag ccaggctttt ctggttagtc agcgtgttca
     5461 gcgcgctgga tttacctgcg ttggaacggc ctgcaaaagc cacttcaatt ccggtatcgg
     5521 aaggtaggtg gcgaatatca ggcgcactca tcacaaaatg cgtctgttga taattcaaat
     5581 tagtcaaagc ggtcgtctcc gtcagtcaaa gctttgtggc gattatacct gaaccataat
     5641 aaaaggcgga tttttcggcg tgagcgttgt aagtaaaaac catacgcttt gtgagacatt
     5701 gccgatagtc tttatgcgaa atagcagaga aaattctgcg atgcatgtca aataagctat
     5761 ataaatcagt gaattgcttt tatataaaac tctgaaaatg tgcaaatact gatggcggtt
     5821 gattgtttgt ttaaagcgaa ggcgtaaagt agcacccata gagcgaggac gctaacagga
     5881 acaatgactc aggatgaggg tcaggagcgc caggaggcga agacagagga ttgtcaggaa
     5941 gacaaacgtc cggagacgta attaaacgga aatggaatca acacggattg ttccggctaa
     6001 aggaaaaaca gggtgtgttg gcggcctgca aggattgtaa gacccgttaa gggttatgag
     6061 tcaggaaaaa aggcgacaga gtaatctgtc gccttttttc tttgcttgct ttctgttaga
     6121 ttccgccgca aatcgatact gaataaaacg gctaaaagac gaaccattat gaaaccatca
     6181 tcttcaaact cacgcagcaa aggtcatgca aaagcgcgtc gaaaaacacg cgaggagctg
     6241 gatcaggaag ctcgtgatcg caagcgtcag aaaaaacgtc gtggtcatgc gccgggcagc
     6301 cgcgcagcgg gcggaaacac cacgtcaggc agcaaaggcc agaacgcacc aaaagatcca
     6361 cgtattggca gtaaaacccc tattccattg ggcgtgactg aaaaagtcac caaacagcac
     6421 aaaccgaaga gtgagaaacc tatgctttca ccgcaggcgg agttggaatt actggaaacg
     6481 gatgagcgtc tggatgcgct gctggaacgt ctggaagcag gcgaaaccct gagtgccgaa
     6541 gagcaatcct gggtggatgc taaactggat cgtattgatg agttgatgca gaaactcggc
     6601 ctctcttatg acgatgacga agaagaggaa gaagacgaga agcaagaaga catgatgcgt
     6661 ctgctgcggg gcaactaacg gattgccgcc gtgggccttc ccgttctgct gataaccctt
     6721 ccggttatat gttatctggt gtggttattc gttaaactac agcggttgtc gcggcgacaa
     6781 aagtggctgc gcaaccggct gatgacccga aacgggcatc cgccggtacg ccgtagccgc
     6841 cagagacgcc atcggaagga gtgagcatgt ctgtacagca aatcgactgg gatctggccc
     6901 tgatccagaa atataactat tccgggccac gatacacctc gtacccgacc gcgctggagt
     6961 tttcagaaga cttcggcgaa caggcgtttt tacaagccgt ggcgcgctat cctgagcgtc
     7021 cattatctct ctacgtacat atcccgttct gccataagct ttgttacttc tgcggttgca
     7081 ataagattgt tactcgccag cagcacaagg ccgatcagta tctggacgcg ctggagcaag
     7141 aaatcgtcca tcgtgcaccg ctgttcgccg ggcgtcacgt cagccaattg cactggggcg
     7201 gcggaacgcc gacgtatctg aataaagcgc aaatcagccg cctgatgaag ctgctgcgcg
     7261 aaaacttcca gttcaatgcc gatgcggaga tttcgatcga agtcgatccg cgggaaatcg
     7321 aactggatgt actcgatcat ttacgcgccg aaggctttaa tcgcctgagc atgggcgtgc
     7381 aggacttcaa caaagaagtg caacgtctgg ttaaccgcga gcaggatgaa gagttcatct
     7441 ttgcactgct taaccatgcg cgtgagattg gttttacctc caccaacatc gacctgattt
     7501 acggcctgcc gaaacagacg ccggagagtt tcgcctttac cctgaaacgt gtggcggagc
     7561 tgaaccccga tcgtctgagt gtctttaact acgcgcatct gccgaccatt tttgctgctc
     7621 agcgcaaaat caaagatgct gacctgccga gtccgcagca aaaactcgat atcctgcagg
     7681 aaaccatcgc cttcctgacg caatcgggct atcagtttat cggtatggat cactttgccc
     7741 gtccggatga cgagctggcg gtggcccagc gtgaaggcgt gctgcatcgt aacttccagg
     7801 gctacaccac tcagggcgat accgatctgc tggggatggg cgtttccgcc atcagcatga
     7861 ttggcgactg ctacgcgcag aaccagaaag agttgaagca gtactatcag caagtggatg
     7921 aacaaggcaa tgcgctgtgg cgtggtattg cgctaacgcg tgatgactgt attcgccgcg
     7981 atgtgattaa gtcgctcatc tgcaacttcc gtctggatta cgcccctatt gagaaacagt
     8041 gggatttgca cttcgctgat tactttgcgg aagatctcaa gctgctcgcc ccgttagcaa
     8101 aagatgggct ggtggatgtg gatgagaagg gaatacaggt gacggcgaaa ggtcgcttgc
     8161 tgatccgcaa catttgcatg tgctttgata cctatctgcg ccagaaagcg cggatgcagc
     8221 agttctcacg ggtgatttga atagtgaatg gcgcttcgtt tacaaagtag aaaacgaaag
     8281 cgccatcaat gtgctgagaa caagattgcc tgatgcgcta cgcttatcag gcctacaaaa
     8341 cctgttgaaa atattgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     8401 caaagagcac ttcatcagca atctgaaact caactaaaca gcccaatcat tgcggcacac
     8461 agcacggcag caacggcagt ttgcggtgtg tggctgtcaa ctgcgccact cgataccaga
     8521 ttaattattg attccagcat aatgactctc cccgttttcc gggcagatca tactgaactt
     8581 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8641 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     8701 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgcaac gcggtcgtca
     8761 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     8821 gcgctctgtc tgcccactgc gcaagaagcg tcgcccagct gtccggttgc atttgcgaag
     8881 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     8941 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9001 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9061 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9121 gttcgcgcag cggcggcaga tgaacgcgga taacgtttag gcggtggaac agatcctcac
     9181 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatccgca
     9241 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9301 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9361 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9421 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9481 cgataaacgg tgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9541 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9601 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9661 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
     9721 gcgccacggc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
     9781 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
     9841 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
     9901 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
     9961 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10021 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10081 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10141 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10201 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10261 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10321 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10381 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10441 gctgggatcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10501 taccacgcgt tcagccactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10561 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10621 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10681 accgccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    10741 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    10801 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    10861 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    10921 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    10981 caatttgcgg gagctttggg cgagcagttg ttgcgcggca gggttggcgt aatggatctc
    11041 caggttgtca tcgattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11101 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11161 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11221 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11281 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11341 cgcacgcggt catcttcttc gcgacgcaga gcgatgtacg catcaattgc ttcgtcagtg
    11401 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11461 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11521 ttgtccatgg cttcacccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11581 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11641 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    11701 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    11761 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    11821 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    11881 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    11941 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12001 tcgtcagctt ttttggtcat ggtattgaag cgggtagcca cttcgttctg accagcagtc
    12061 gctacttcgt ggtgatgggc ttcaaccacc agccccatct gttccatcac cagacacatt
    12121 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12181 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12241 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12301 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12361 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12421 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12481 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12541 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12601 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12661 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    12721 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    12781 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    12841 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    12901 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    12961 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13021 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13081 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13141 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13201 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13261 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13321 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13381 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13441 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13501 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13561 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13621 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13681 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    13741 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    13801 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    13861 gtatcgaaac cgacctggcg gaagctggcg atatcgttgc gatcacgggc cttggcgaac
    13921 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    13981 ttgatgagcc gactgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14041 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14101 acgttgcgct gcgcgtagaa gaaaccgaag acgctgatgc gttccgcgtt tctggtcgtg
    14161 gcgaactaca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14221 tatcccgtcc gaaagttatc ttccgtgaaa tcgacggtcg taaacaagag ccatatgaaa
    14281 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14341 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14401 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14461 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14521 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcgctgt
    14581 tcggtctgca ggatcgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14641 agattatcgg tattcatagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    14701 aactgaccaa catgcgtgct tccggtactg acgaagccgt tgttctggtt ccgcctatcc
    14761 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    14821 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    14881 gcgcaccgaa agactattaa tttcgttttt tagttataaa aaacctgcca tcgctg
//
LOCUS       DAJVOY010000095        14563 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVOY010000095 DAJVOY010000000
VERSION     DAJVOY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.095"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQL90_002546"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQL90_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1369283.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQL90_002547"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQL90_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1369284.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQL90_002548"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQL90_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1369285.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQL90_002549"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQL90_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1369286.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQL90_002550"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQL90_002550"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1369287.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQL90_002551"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQL90_002551"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1369288.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQL90_002552"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQL90_002552"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1369289.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OQL90_002553"
     CDS             complement(4598..5320)
                     /locus_tag="OQL90_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1369290.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQL90_002554"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQL90_002554"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1369291.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OQL90_002555"
     CDS             6520..6738
                     /locus_tag="OQL90_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1369292.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQL90_002556"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQL90_002556"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1369293.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OQL90_002557"
     CDS             complement(7472..7672)
                     /locus_tag="OQL90_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1369294.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OQL90_002558"
     CDS             complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OQL90_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCS1369295.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEE
                     DEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYY
                     GDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHE
                     LLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHA
                     DTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQL90_002559"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQL90_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1369296.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OQL90_002560"
     CDS             10175..12088
                     /locus_tag="OQL90_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1369297.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OQL90_002561"
     CDS             12088..13110
                     /locus_tag="OQL90_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1369298.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OQL90_002562"
     CDS             13104..13322
                     /locus_tag="OQL90_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1369299.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OQL90_002563"
     CDS             13376..14245
                     /locus_tag="OQL90_002563"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1369300.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..>14563)
                     /locus_tag="OQL90_002564"
     CDS             complement(14300..>14563)
                     /locus_tag="OQL90_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1369301.1"
                     /translation="SAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIV
                     TGRDLKDAAVARAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3601 a         3971 c         3585 g         3406 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcagt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccaag tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ttcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ctgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccggcgt tatcgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc aaggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcaacaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcaag cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gcgacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtgatatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc cctcgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atattatgcc gcgcagtcag tttcgcgaat
    13321 aacctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga agcgaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg aacattcacc ccctggcaac ctttaccaga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtagtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctg gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcggc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atcgaaacca catcgatggc act
//
LOCUS       DAJVOY010000096        14525 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVOY010000096 DAJVOY010000000
VERSION     DAJVOY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.096"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(98..889)
                     /gene="fimH"
                     /locus_tag="OQL90_002565"
                     /pseudo
     CDS             complement(98..889)
                     /gene="fimH"
                     /locus_tag="OQL90_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
     gene            complement(909..1412)
                     /gene="fimG"
                     /locus_tag="OQL90_002566"
     CDS             complement(909..1412)
                     /gene="fimG"
                     /locus_tag="OQL90_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCS1369302.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1425..1955)
                     /gene="fimF"
                     /locus_tag="OQL90_002567"
     CDS             complement(1425..1955)
                     /gene="fimF"
                     /locus_tag="OQL90_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCS1369303.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1965..4601)
                     /gene="fimD"
                     /locus_tag="OQL90_002568"
                     /pseudo
     CDS             complement(1965..4601)
                     /gene="fimD"
                     /locus_tag="OQL90_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(4668..5393)
                     /gene="fimC"
                     /locus_tag="OQL90_002569"
     CDS             complement(4668..5393)
                     /gene="fimC"
                     /locus_tag="OQL90_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1369304.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5430..5969)
                     /locus_tag="OQL90_002570"
     CDS             complement(5430..5969)
                     /locus_tag="OQL90_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1369305.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(6034..6582)
                     /gene="fimA"
                     /locus_tag="OQL90_002571"
     CDS             complement(6034..6582)
                     /gene="fimA"
                     /locus_tag="OQL90_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1369306.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(7063..7659)
                     /gene="fimE"
                     /locus_tag="OQL90_002572"
     CDS             complement(7063..7659)
                     /gene="fimE"
                     /locus_tag="OQL90_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCS1369307.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(8137..8739)
                     /gene="fimB"
                     /locus_tag="OQL90_002573"
     CDS             complement(8137..8739)
                     /gene="fimB"
                     /locus_tag="OQL90_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCS1369308.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            10195..10911
                     /gene="nanC"
                     /locus_tag="OQL90_002574"
     CDS             10195..10911
                     /gene="nanC"
                     /locus_tag="OQL90_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1369309.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10931..12037
                     /gene="nanM"
                     /locus_tag="OQL90_002575"
     CDS             10931..12037
                     /gene="nanM"
                     /locus_tag="OQL90_002575"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1369310.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            12102..13082
                     /locus_tag="OQL90_002576"
     CDS             12102..13082
                     /locus_tag="OQL90_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1369311.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWREK"
     gene            complement(13090..13212)
                     /locus_tag="OQL90_002577"
     CDS             complement(13090..13212)
                     /locus_tag="OQL90_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1369312.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            13294..14166
                     /locus_tag="OQL90_002578"
     CDS             13294..14166
                     /locus_tag="OQL90_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1369313.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            14211..14390
                     /locus_tag="OQL90_002579"
     CDS             14211..14390
                     /locus_tag="OQL90_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369314.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISWAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         4074 a         3352 c         3175 g         3924 t
ORIGIN      
        1 agaagcagaa aaagcttatt atgcttccat cggaaacgat gatctggcag cctgggttca
       61 cagataaaac actctccagg aaacccgggg cggttcatac tgctcctaac gataccgtgt
      121 tattcgctgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc tgcgctggtg
      181 aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg ccggagaggt
      241 aatcccccag gttttggctt ttcgcacaat aaacggtaag aggaattggc actgaaccag
      301 ggtagtccgg cagagtaacg gtgacatcac gagcagaaac atcgcagccg ccagtaggca
      361 ccaccacatc attattggcg taaatattcc acacaaactg gaaatcatcg ctgttatagt
      421 tgttggtctg tcgcaaaata agcacggcaa ttaatgagcc agctttaatc gccaccccgc
      481 ccgcactgct cacaggcgtc aaataaagcg ccaccggcca cggcttatcc gttctcgaat
      541 tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg atagctactg ccactatatt
      601 ttacggtccc ggaaaaatta gataacacgc cgccataagc cgagcctcgt tgcagtgtga
      661 catagtctgt aatggtttcc ggataatcgt tatggcaaaa gatttgcgtc gaaagatcta
      721 cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt tacataaaca ttagcgctgc
      781 caccgccaat agggatagca gtaccattgg cggttttaca ggcgaatgac caggcattta
      841 ccgaccagcc catcagcagt acagcaaaca gggtaataac tcgtttcatt acaattatct
      901 cttcgggttc agctgtaggt ataggtgatg ctaatcactg cctgaatggt tccctgagtg
      961 gctccgccat ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga ttgtgaggaa
     1021 tcatccacct gaactgtttt ggtttcgcca gtattcaatg tgttgccact gtcatcctgt
     1081 agctctaact ggatgttttg cgcggtcccc tggtttttat aatatccggt actgtcggct
     1141 gccccgctga agctggcagt gaccctcgac gttcccaccg gacaattagt caactcaagc
     1201 gcaacatcat gccaggccga tgccgccccg gcagacatca gactgaaaga ataaagatcg
     1261 ccgagatcaa ccgtggcatt ggtggtggaa actgtgcacg gtttggcgac gaccttaccg
     1321 ttcaccgtga tggtgacatc ggctgcctgt attgtcgcac ttgcgagcgc caatattgcc
     1381 gccaataaat acccacgttt gcgccatttc atgagcatct ccagttactg atattcaaga
     1441 gtgaaggtag ccgtggcatt gatatgcccc gcagtgacag gcacctgtgt cgccattagc
     1501 cgggcgtaaa aattcaacgt atttggttta cccggcgtca gggtcgtcca cgaaatcgcg
     1561 gacgatggag cattaagggg tatctgattt tgctgctcat tcagaagctg tattcccagt
     1621 cccgaagccg ctgacaccgt attttcaagt gcaagcaggt tggcattgtg gctatctgca
     1681 acgccggtaa acccaacctt tacggcagaa acggcgttac cacagggtga cagcaaaata
     1741 cgaaatggaa cgacaggagt cgttgcgcca atgttgttaa attgcttcgc cgcgttttcc
     1801 atcagatcaa cagtaaaatt ggttgattca gcggccacac tacagccgtt atctctgaca
     1861 tagccgcgga tagtaatcgt gctatccgca gccaaaacgc gacttaccgc cagccacaaa
     1921 aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc ccccttaacg acattcagct
     1981 gatagctggg ttaataactg ctgctgactc tctggtggca gttgataatt ggcgatacag
     2041 tgagcatttt cctcttctcc ccatttcacc tgaacttttc ccgctaaagg cattccgctg
     2101 aggtaaacct gaccattatc cgcaacaatg ccgctactct ggctactctc tgatgtcacc
     2161 atcgccccaa acggcagcgg cttattattg tgggttagcg tcatgagcag ttttatccca
     2221 acgcgcgctt taaactctgc tcgcacgatc gccccacgag tgggaacaac gttagcgacc
     2281 gcgttatcta aatcgacgtt atcagccagg gtattggtat ccagcgccac tctgttttcc
     2341 cgatattcgg tggcataagg cagcacggca taaccgcgcc agtcggtacg cacccccgtc
     2401 tggttttcga cttttgcatc ttttgcgcca ggcgctttaa caagtaccac cgtatcgttt
     2461 aacggctgcc ccagcgttac gccattggca tgagccagta ccccaccgct gactccgtaa
     2521 tagagctgct taatatcatc gctatggctg taaccgatat tggcattgcc gtaaccaccg
     2581 cgataattca gcgtggcgta gcctgtactt ccgctattac catcgcctcc cccggcatag
     2641 ccggtttgca cgctatagct gaggttgttg tcttccagca acgtaccgta tacaccagcc
     2701 agattggtca tccgaccgtt gagatcgtgt gacacgctgt agctggcact ggcatgtcgc
     2761 cactgagatt tactgtcaga acgcagccag tggctgaaag gaatattgac gttaagcgct
     2821 aacatctgat cccgcccttt ttgccaggcg tttttcgtca ggctatagct gagcgtccag
     2881 ttgatatctt cgaacgcagt atttaatcca gcctagaatt gctcatcgac attactcgtt
     2941 ccccaataag tttgatggct accactcaaa tacagtgttg atgtgcgccc gagttgctga
     3001 gtaacggtga gctgtaattt cccgcgtttg ttataagcga ggttgtaata gtcggtgaat
     3061 ttcggcttaa cctgaataac tccatcctgt gtttcgatgt tgtagccatt cattcgactg
     3121 tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat aacggtaacc cactaactga
     3181 atattcgtgc ctgactcatt gagcgatttg ttatagagaa aacgcaccga ttgtccgtca
     3241 tgctgactgt catcgggaag tgtggaatta gcctgcgtca tatccacaga cagagcgccc
     3301 agtgccccca tgtttttccc gataccgaaa ttaaaagcac gataacgatc cgccagttgc
     3361 gttccaccat atattgtcca gccagccgga aggccgtgga gtaatgtact ttggaaaaag
     3421 cggggttttt cctgctgcgt atttccacta cggtattctc ctgccgtaat ggaataacga
     3481 gtatgccctt cacgttgcaa aagcgggact gacgaatagg gtacggtaaa aatctgcgtg
     3541 ctgccgtcag cctctttgat cgttacctgc aagtcaccac tattacctgc ggcatagata
     3601 tcgttgatgg taaaaggccc cggtggcacc gtactattat aaatgtcata cccattttgt
     3661 ttaatagtga cctgtgcagt accacgagca ataccgtgga tcaccggggc aaatcctctt
     3721 tggctatcgg gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa gttaatacca
     3781 tcgaaaatat cgccctgggt ataaccatca cccagcgtca gccgggaacg taacggtatt
     3841 atgtctcgct caagccaggt attgatatgc tgccatttat ttttgctacc tgatgatctg
     3901 tcgctactgt tataactcca gctggtattg tcgcgtaaac accacgcacc aatatttaac
     3961 ccactctgta ggtttaaata tgcataatgg ctgttacccc caatctgatt ctgtacacta
     4021 tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca taactcagga
     4081 ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag gttcagtcgc
     4141 tgctgaccaa catccagatg cgcagtagcg tcatggatca ttgaggttaa tggcacgcag
     4201 gcatcatctg ccagcagatt cataccggag acagaagccg cattcagccc catactggca
     4261 agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt attaaatgtg
     4321 acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata cgtccctggc
     4381 ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg atcatccgct
     4441 aaaaagcgcg gattaaaata gagttcggca gatgacaaag gtgcctgtgc ggcaaaagca
     4501 caggcgacaa cgagccggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat
     4561 tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaaat
     4621 tacccggcag tcaattcttt ttattcaggc gagaaattcc ccctgcgtta ttccattacg
     4681 cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt aatattgctt
     4741 cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa tgcattttca
     4801 agaacccggg ttccggcatt caactctgtt accgtcagat aatagggtgt cgggttaatc
     4861 agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg atcgggtggc
     4921 aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc gaactgtagc
     4981 gtattctcag tcaattttga tttatccatt gacggaatcg ctttaacatt catccagaat
     5041 aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg caaggtattc
     5101 tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc atcctttaca
     5161 ccatcggcat tttccatcca tgattgaatt aaataggtac tattttcatc attatttgtc
     5221 acggcaagtt gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc acctaaggcc
     5281 actcccgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat acctgccagc
     5341 aagcagaatg ttatttcctg cgatttcctt acattggcgt ttttattact cactgtcctg
     5401 ttcctgttat cacattatct gctgaacaat tactgatagg ttaaagagaa ccaggcctgg
     5461 gcattggcga tgccgccagt aacccaacgc ccggtagcac gatatttggc gatgaaatgt
     5521 agcgaagtag agcctgagta aagccgtttc cagtttactg gaggacgatt aatcggtacg
     5581 aggtttcctt catcatcaaa caacgctacg ccaatattgg tggctatccc tggcccctct
     5641 cccacggaaa gcacatccgg atttttacca tccgcgacac cgtgaaacgc cacacccaca
     5701 cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac cggtgcgcta
     5761 tcttccccta ccgcatgaaa ccggttactg ctgatttgcc ccatattgac cgtcatttgt
     5821 ttatcaccgg cttcaatccg gcaagtttcc gcaataatga cgccctgaaa ttgcatattt
     5881 ccgccgggca gcgtagtatt ccatttattt ccggccagag caaacattgg cagcaacgac
     5941 gctaatagaa ataatctctt ccttttcatc gttattttta tcgcacaagg gtgggcatcc
     6001 ctgcccgtaa tgacgtccct gaacctgggt aggttattga tactgaacct tgaaggtcgc
     6061 atccgcatta gctgcgccag gggttgcgac accggttgca taataacgcg cctggaacgg
     6121 aatggtgtta gtaccgttat tcagggttgt ttgctcactg aatgtcgcac catccagcgt
     6181 cagcgcagca cccgttctgt ccaggatctg tacaccaacg tttgttgcgc tacccgcagc
     6241 tgaactctgc agagccagaa cgttggtatg acccgcatca atcgccgtac ctaaaaaggc
     6301 aactgcggct ttagatgcaa cattggtatc gcaatcattc agctgaatgt taaaaccgac
     6361 agcagaactg gttgctcctt cctgtgccag cgatgcggta cgaacctgtc ctaactgaac
     6421 gatttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt cccctttaaa
     6481 gtgaacggtc ccaccattaa ccgtcgtggc agcggccaga gccgctgtag aactgaggga
     6541 cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt cctttaaaaa
     6601 aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc aacgagtttg
     6661 actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca ttttgactca
     6721 tagaggaaag catcgcgcac aaactttttc agtttatttg ttggcttaat attctattgt
     6781 tatctttatt tatagatttt tatattgcat gaggtggttt ttggagagaa gaatgaggaa
     6841 gatgcgtcga gccacagaaa cgttagcttt acatatagcg gaggtgatgt gaaattaatt
     6901 tacaagagaa ataatttaca tatcaaacag ttagatgatt tttgtcgttt tttaaaattt
     6961 ttatgcttga gaaaaaatac gtaacttatt tatgatatgg acaatttggc cccaattgtc
     7021 ttgtatttat ttatttttta actttattat caataagtta attcaaacct cttctctttt
     7081 taatttttcg tttatgagat tatttctttc ccataatccg gcaaaacgag cagcattact
     7141 ggcggtataa cgtacagtat ggcgaatatt tcgatgcccg agataatcct gaattaaacg
     7201 agtatctgca ccacgctccg ccagttcata accgcaagca tgccttaaca tatgaggatg
     7261 agtctgcgtt acggttccag cttcaatacc ggcatcacga ataatgcgat aggcctgctg
     7321 gcgagaaagc cgactcccgc ggcgagaaat aaatatggcg tcggtccggt cagcgccttt
     7381 ccagttagca cgttccaggg tccagcgttc cacagcttca cgctcatcaa aacgtaacgg
     7441 gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt attcgacctt cattaaggtc
     7501 aagatcctga taatgcagat caagcagttc actaatacgc atcccatgcc gatatgccaa
     7561 cagaataaga caataatctc tggctcccgt tgccccgtaa caaaccgcct gcatcatggc
     7621 ctgaacttct ttaccggtaa gataacgacg tttactcaca atagtagtac tcctaactga
     7681 gatatatttg aatgaatacc tataggaaac ctcaatcggt caaaattagc ccaatagata
     7741 aaaagataac cacacacgaa gtgatgtggc tatcagtcaa ttacattaaa tatcatacaa
     7801 aaataaaata tcactgatgt gacaacatct attttcattg atttgattca atataacaat
     7861 agattgtagt agattagctt ccagttgctt aaaaaataaa gaaaaaagtg cgaaaacagg
     7921 agaatttaag tgatgtgtaa atttatgaat gattttgttt atttattttg aagaacataa
     7981 accattgtat tttgtatgga tatggttaac ttaatgctta ttatgataat taagaattat
     8041 acttacaata acatcaatta ataaatggtt aatcattttt gatatatcgt aagaataatg
     8101 tagtttttaa caccatccct ggtatctcaa ctatctctat aaaacagcgt gacgctgtcg
     8161 tcctctggct ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg tataccagac
     8221 agtatgacga atattgcgat gcccaagata atcctggata agtcgcgtat ctattcccat
     8281 attcgccaaa gcaaaaccac acgaatggcg taacatgtgc ggatgaatct ccagtgacaa
     8341 cccggcatta ccaccggaag tcgagataat atggtaaaac tgttgccgag aaagcggatt
     8401 ccccttacgc gataaaaata cccactcgct ctcagcatgc gggtacgaag tacggatact
     8461 caaccagttt tttaaagcct gaacttcttt attcaatagc gggggcgttg ttgaaaagcc
     8521 tttttttaat cgatggatat atatacactt tgccttaaga tcaatatccg aaatcctcaa
     8581 tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa agcaaagtca gacaataatt
     8641 acgtgctgca tgaggcccgg tatttgctgc tttaaggagt gattcgattt cactatgggt
     8701 caggaagttc ctttttttgt tatcagcctt attcttcatc gtttttccct tataattaca
     8761 gacgcgcact agctgtgctg ggttagcaat aatccaacat ttttatcctg attatgttta
     8821 taaaagcgaa cgtttgctta ataactaacg taagtggacc agttcttctg agtgaactta
     8881 aatggggtag caacagttaa ttataataac gttgccatac aaacgctatg ctcttgcatg
     8941 ctatgtacct ttatatattt atcaatcgga tacgaatcta ataatacccc tcatcattaa
     9001 tgcaactaat cttatctata tatcatgtga tatgttctgt aacaagtaaa atcagcataa
     9061 aaaattcagc aaaatttaat attcgttcaa ctagccgtct cacatatgac attattagtc
     9121 aactccattt gccacaaaat ggctatttca tggaacgcag taatttctta gtggctatta
     9181 tcatgctaat tcttaggtcc ctagcgatta ttcctggctg attcgtcagc ttacgccatg
     9241 agattgttac attccttttt atcctggaga tatgggggaa cgcaggagat gattttttca
     9301 tctacattcg ttggtaaata tagctttggt cactacgact taaaccttcc tcaatcacag
     9361 ccattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa aacaccaaca
     9421 tcaacaagcc tctccagatc gacttcagaa gtgaccagtt acaagccaca aaccaggaaa
     9481 catatttcat gatgagaatt atgctcagcc tgatggcggg taaacactga ttttcaccaa
     9541 gctcactgag ttagcttgca aagcttccct tgtttagact ccaaataaat tatttataaa
     9601 acaaacattt aaacaaagat ctttagtttt tgaagaccac cgcaagtgtt cgtctggctt
     9661 cacatggcat cttcctctta gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct
     9721 ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa aggtgcacca
     9781 agatagtcaa tgagacaggg catctcgcaa tctatggcaa acatcacttc agttctttct
     9841 catcgggtga tgaaaacgca cttcagtctg aaaggaatat gaaaatgaga tcaacagaca
     9901 ctctatttta tgactctggg taaaatggat tgagtaagtg atatagctta cgaaaattca
     9961 aatcaattaa acatcagaag agattttata ctcaggtatt taatctggat ctctgtttat
    10021 ttaaataatg tgaaaagaga tttttcacag gagaccttat acaaaaaaat ataaaataca
    10081 gctaccggtt gccaaagaca ctataagcct ggcaaaaaat attacacaac ataaatgcta
    10141 attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa atttatgaaa
    10201 aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt aatttctcag
    10261 gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta tgagactcga
    10321 ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag taatacctgg
    10381 aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca ggttgaagtt
    10441 aattacgcga ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt aacgcatttt
    10501 agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc gacaaaagat
    10561 cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca agacttatcc
    10621 agtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta ccatattaat
    10681 agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaacga ttatcgctat
    10741 gcaaaccata agaaatgggc gacggaaaat gcatttgttc tacaatacca tatgacgccc
    10801 gatattacgc catacataga atatgactac cttgaccgtc agggtgttta caacggcaga
    10861 gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta gtagacagga
    10921 gacagtcaca atgaataaaa caataacggc gcttgctatc atgatggctt catttgccgc
    10981 aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg gagcaattga
    11041 taacgacact gtctacattg gtttaggtag cgcaggtacg gcatggtaca agctggatac
    11101 acaggccaaa gataaaaaat ggacagcgtt agctgcattc cctggtggac caagagatca
    11161 agcaacctct gcatttattg atggcaatct gtatgtgttt ggcggcattg gcaaaaacag
    11221 agagggcttg actcaggtat ttaatgacgt acacaaatac aaccccaaaa ccaatagctg
    11281 ggttaaattg atgtcgcacg caccgatggg catggcgggt catgtgactt ttgtacacaa
    11341 cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct attttgaaga
    11401 tctcaacgag gctggaaaag attcaaccgc tgtagataaa atcaatgcct actattttga
    11461 caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc cctcaacaca
    11521 gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg cggttgtgaa
    11581 taaaggtgat aaaacctggc ttattaatgg cgaagccaaa cccggattgc gaacggatgc
    11641 cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg ctcccgtctc
    11701 atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc ttatatttgc
    11761 cggaggggcc ggattcaaag gttcacgaga aaattaccag cacggaaaga actatgcgca
    11821 tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg ggaaatggga
    11881 taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct ggaataatag
    11941 tctattgatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt cagttttgat
    12001 ctctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc cagtaaaggg
    12061 gccctgctat caatttatca tttcaattac gcgaaaatta tatggacgca ataatatcgc
    12121 ccgattatta ctatgttctt accattgctg gtcagtctaa tgctatggcg tatggcgaag
    12181 gactgccatt accggccagg gaagatgcgc ctcatcccag aattaaacaa ttagcgagat
    12241 ttgcgcatac gcatcccgga ggcccgtcat gtcactttaa cgacattatt ccactaactc
    12301 actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca acgaatcatc
    12361 aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta ctgtccttta
    12421 ttcctgataa cgcaggggtt ctcatcgttc cgtgttgccg tggcggatcg gcttttaccg
    12481 cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct tgtcgttggg
    12541 gaacggatac tccgctatac caggatttag tcagcagaac gcgagccgca ctggcaaaaa
    12601 atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac ttaatgacca
    12661 gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt cgtagggatc
    12721 taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt tgcggcgata
    12781 ccacctggta ctggaaagaa aatttccctc atgcgtatga agctatttat ggcaattatc
    12841 aaaataatgt tttagctaat attattttcg tcgacttcca gcaacaaggt gaaagaggac
    12901 tgacgaacgc gcctgatgaa gacccggacg atttaatcat gggatattac ggttcagcgt
    12961 accgctcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc gcggcagccc
    13021 gtcgggggat tatttctgac aggtttgtag aagcaatttt gcagttttgg cgcgaaaagt
    13081 gagcgttcat taatttgatt tattcgcatc catcaacgct tttaactcct ccggcgtaaa
    13141 cggtggtttg cgtcggtgca acatcgcatg gcaattaggg cacacaagca gcaaaaaaac
    13201 aaatgagatc aaggtaatat atcttcactt gcaggcttga acagcagaaa atttgcgagc
    13261 atgatcgcaa ttatttcact gctagggata tcaatggctt ccagtaaatc gctacagcag
    13321 gcaattgcca atataaaaat ctggcataag ggtgaacagc gcgcaccgca taaaccattg
    13381 ttattgctat acgtattagc gggatacctg aatggacatc cacgcctttt cgattatgga
    13441 tcggaaatct acgaacctct gcacagttta ctggaacgtt ttgggccaca gcgttcacag
    13501 taccgacctg atatgccgtt ctggcgatta caaggcgacg gattctggca attacacaat
    13561 gccgagcttt gctcaacagc agggagcagc cgacaaccgc cagtgaagga actgaacgaa
    13621 taccacgttg caggcggttt cgatgaacag cactacgccc tggtaaccgg caataaaaaa
    13681 ctcatcaata ctctagcgca acagatactc gaagcgcact tcaccgagag tattcaggaa
    13741 gaaattgctg acgagctggg ttttgatctc cagcaaatcc gtaaacagcg cgacccgtta
    13801 tttcgcaaaa atgtattacg cgcttataac taccagtgcg cgatatgtgg ctttaatatg
    13861 cgccacgacg acaccaccgt cgcgctggaa gcggcgcata taaaatggaa acagcacggc
    13921 ggtccttgtg aaattcctaa tggcctggcg ctttgcgcaa ttcaccataa agcctttgat
    13981 aaaggatcga ttggcctgga cgaaaatatg cgggtactgg tatcagatgc agtaaacggc
    14041 ggagggagcg ttgagagatt attctgggat tttgacggga aaattattgc gttgccgcag
    14101 gtgcgtaaaa attaccctta tgaggggttt gtggagtggc atcggaatga ggtttttagg
    14161 gggtaaggtt gataactatc gcagtatgtt caatattgct gacataatca gtgcaggatc
    14221 ttgccgctag tttacaactc gccttcgata gcggcgcaaa acgagttctg ttgccgatat
    14281 cctgggctat ggatattcca acagttccgg cagagttgtt taccaagttt caggtaagtt
    14341 tttactcaga accggttgat gcggtttata aggcgctgga tgtgaattaa cacattcctc
    14401 agcttattta caaaaggatt attcccattt gatataatat cagtgccaat ttactttttt
    14461 caatggaata tatctcggct aaataacaat aaaacttatc gctaatggat taaataaaca
    14521 atttt
//
LOCUS       DAJVOY010000097        14522 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVOY010000097 DAJVOY010000000
VERSION     DAJVOY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.097"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..420
                     /locus_tag="OQL90_002580"
     CDS             <1..420
                     /locus_tag="OQL90_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1369315.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            436..918
                     /locus_tag="OQL90_002581"
     CDS             436..918
                     /locus_tag="OQL90_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1369316.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(995..1342)
                     /gene="rplS"
                     /locus_tag="OQL90_002582"
     CDS             complement(995..1342)
                     /gene="rplS"
                     /locus_tag="OQL90_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1369317.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1384..2151)
                     /gene="trmD"
                     /locus_tag="OQL90_002583"
     CDS             complement(1384..2151)
                     /gene="trmD"
                     /locus_tag="OQL90_002583"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1369318.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2182..2730)
                     /gene="rimM"
                     /locus_tag="OQL90_002584"
     CDS             complement(2182..2730)
                     /gene="rimM"
                     /locus_tag="OQL90_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1369319.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEDYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2749..2997)
                     /gene="rpsP"
                     /locus_tag="OQL90_002585"
     CDS             complement(2749..2997)
                     /gene="rpsP"
                     /locus_tag="OQL90_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1369320.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3134..4495)
                     /gene="ffh"
                     /locus_tag="OQL90_002586"
     CDS             complement(3134..4495)
                     /gene="ffh"
                     /locus_tag="OQL90_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1369321.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4662..5453
                     /locus_tag="OQL90_002587"
     CDS             4662..5453
                     /locus_tag="OQL90_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1369322.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5474..6760
                     /locus_tag="OQL90_002588"
     CDS             5474..6760
                     /locus_tag="OQL90_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1369323.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6815..7408)
                     /gene="grpE"
                     /locus_tag="OQL90_002589"
     CDS             complement(6815..7408)
                     /gene="grpE"
                     /locus_tag="OQL90_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1369324.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7531..8409
                     /gene="nadK"
                     /locus_tag="OQL90_002590"
     CDS             7531..8409
                     /gene="nadK"
                     /locus_tag="OQL90_002590"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1369325.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8495..10156
                     /gene="recN"
                     /locus_tag="OQL90_002591"
     CDS             8495..10156
                     /gene="recN"
                     /locus_tag="OQL90_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1369326.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10305..10646
                     /gene="bamE"
                     /locus_tag="OQL90_002592"
     CDS             10305..10646
                     /gene="bamE"
                     /locus_tag="OQL90_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1369327.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10708..10998)
                     /locus_tag="OQL90_002593"
     CDS             complement(10708..10998)
                     /locus_tag="OQL90_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1369328.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10988..11425)
                     /gene="ratA"
                     /locus_tag="OQL90_002594"
     CDS             complement(10988..11425)
                     /gene="ratA"
                     /locus_tag="OQL90_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1369329.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11596..12078
                     /gene="smpB"
                     /locus_tag="OQL90_002595"
     CDS             11596..12078
                     /gene="smpB"
                     /locus_tag="OQL90_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1369330.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12293..12655
                     /gene="ssrA"
                     /locus_tag="OQL90_002596"
     tmRNA           12293..12655
                     /gene="ssrA"
                     /locus_tag="OQL90_002596"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12816..14522)
                     /locus_tag="OQL90_002597"
                     /pseudo
     CDS             complement(12816..14522)
                     /locus_tag="OQL90_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3584 a         3898 c         3424 g         3616 t
ORIGIN      
        1 ttgatgaaca attccgatgc ccgaaaaacg tcggcgttac tatttcttga tggcgataat
       61 ttcaaatata tcaatgatac ctggggtcat gcgacgggcg atagggtctt gattgaaatc
      121 gcaaaacggt tagctgaatt tggcgggctg cgacataaag cataccgcct gggcggcgat
      181 gaattcgcta tggtgctcta tgatgtacag tcggaatctg aagtgcaaca gatatgctca
      241 gcactgacac aaatctttaa tctcccgttt gatcttcata atggccatca gaccaccatg
      301 acattaagca ttggttacgc gatgaccatt gagcacgcct ctgcggaaaa attacaagag
      361 cttgccgatc acaatatgta tcaggccaaa caccagcgtg ccgaaaagct ggtgagataa
      421 caaggatata tcgatatgat aaagcacctg gtagcccccc tgattttcac ctcactaata
      481 ctgactggct gccagtcccc tcagggaaag tttactcctg agcaagtcgc cgctatgcaa
      541 tcttatggat ttactgaatc cgccggcgac tggtcgctgg gcttatcaga tgccattctg
      601 ttcgcaaaaa atgactacaa attgctcccg gaaagccagc aacagatcca aaccatggca
      661 gccaaattgg cctcgacagg gctaacacat gcccgtatgg atggacacac cgataactat
      721 ggtgaagaca gttacaacga aggcttatca ttgaaacggg cgaatgtcgt ggccgatgca
      781 tgggctattg gtggacaaat tccacgcagc aatctcacca cacagggttt aggaaaaaaa
      841 tatcccatag ccagtaacaa gaccgcccag ggccgcgccg agaaccgccg cgtcgcagtg
      901 gtgattacaa ccccttaaaa cacgagcaaa cagacaaaaa aagggccagc caattggcca
      961 gcccttctta acaggatgtc gcttaagcga aatcttagtt aagacgctct ttgatacgag
     1021 cagccttacc agtacgctca cgcaggtagt acagtttagc tttacgaaca gcaccacgac
     1081 gtttgacaga aatgctgtca actaccggag agtgagtctg gaagacacgc tcaacgcctt
     1141 cgccgttgga aattttacga acagtgaatg cagagtgcag accgcggtta cgaatagcga
     1201 taaccacgcc ctcgaatgcc tgcagacgtt ttttggaacc ttcaacaacc catactttca
     1261 cttccacggt atcacccgga cggaaggaag gtacgtcctg cttcatctgc tcttgttcaa
     1321 gttgcttaat aatgttgctc ataatttaat ctcttatcct gggtaaactg atatctcggg
     1381 ggcttacgcc atcccatcat gtttatgttg ctgttgtgcg tgttccgttt tgaactccgc
     1441 cagcaacctt gcttgctctt cagtcagagc caggttttcc agaagttcag gtcttctaag
     1501 ccaggtacgg cccagcgact gtttcaaacg ccagcgacgt atctcggcat ggttgcccga
     1561 cagtaacact ggcggaactt ccatcccttc taacacctca ggccgcgtat agtgcgggca
     1621 atccagcaat ccttcagcaa aggaatcttc cgttgccgag gcttcatgtc ccagtacccc
     1681 cggaataaac cgggaaacgg agtcaatcag cgtcattgct ggtaactcac caccactgag
     1741 aacgtaatcg ccgattgacc attcttcgtc aatttcggtt tggatcacgc gctcatctat
     1801 accttcgtag cgaccgcaca ccagaatcaa tttttgattc gttgccaatt cgctgacgcc
     1861 cgcttgatca agcttgcgtc cctgtggtga cagataaatc acctttgcgc cttcacccgc
     1921 cgcggctttt gctgcatgaa tggcgtcccg caagggttgc accatcatta acatccccgg
     1981 tccgccgccg taaggacgat cgtccacggt acggtgccgg tcatgcgtga agtcgcgagg
     2041 actccagctc tggatgctca gcaggccatt tttaactgcc cggccagtta ccccgtaatc
     2101 ggtaattgcg cggaacattt caggaaacag gctaattatg ccaatccaca tagcgccgtc
     2161 ttttaccgtt tatccggtgg tttaaaaacc aggatcccaa tctacttcga ttgaacgagt
     2221 agtgagatcg actttcttga taacctgccc atcgaggaac ggtacgagac gttccttgat
     2281 accaaacgca tctttcaggt ttgccttaat gacgagaacg tcattagatc cggtttccat
     2341 catatcgacg actttaccga gatcgtagtc ttcagtggtt actacctggc agcccatcag
     2401 gtctttccag tagtagtcac cctcttcaag ctgaggcaac tgcgatgaat ccacgacaat
     2461 ttcacaattc gtcagcaggt tcgccgcatc acgatcgtca acgcctttca gcttgatgat
     2521 catgtcctga ttgtggtgct tccagctttc cagctggact tgctgccact gacccgcctt
     2581 ctggataaac cagggctgat agtcaaaaat gctttcggcg tcttcggtgg aagaaaacac
     2641 tctgagccac ccacgaatac cgtaagacga acccattttt cccaaaacga tgggatcaac
     2701 aggtgcttgc gcggtgagtt gtttgctcat catgaccacc gtgacagatt aagctgcttt
     2761 gtttacttct ttgatcagcg cagcaacgcg atcagaaata gttgcgccct ggccaaccca
     2821 gtgagcgatg cgatccagat ccaggcgagt gccttcttct ttttcgctag cgattgggtt
     2881 gaagaaacca acgcgctcga tgaagcgacc gttgcgtgca ttacggctgt cagcgacaac
     2941 aacctggtag aacggacgct ttttagcgcc gtgacgtgct aaacgaatag ttaccataac
     3001 atcctcttgt gtgaataaaa caaccggacc ccatcgagga acggagtccg gtgtcatatt
     3061 aaaagcccga aaattttact gatttctgga gaaaatgcaa tcagcagtta ataactctgt
     3121 cgcataaggt catttagcga ccaggaaatc ccggcggcat catccccttc atgcttctca
     3181 tcatcttcgc cattccgccc ttcttcattt tcttcatcat gcgctgcatg tcgtcgaact
     3241 gtttcagaag acggttaacg tcctgcacct gcataccgca accggcagca atacggcgtt
     3301 tacgcgaacc tttgatgatt tctggcttag cgcgctcttt catcgtcatc gagttgatga
     3361 tggcttccat acgcaccagc actttatcgt ccatctgtga cttgacgtta tccgggatct
     3421 gccccatgcc cggcagcttg cccatcaggc tggccatgcc gcccatattt ttcatctggc
     3481 gcagctgctc aagaaagtcg ttgagatcga agccgtcacc ttttttcagc ttgctggcta
     3541 atttctctgc ctgcgcgcgg tcaactttgc tttcgatatc ttcgatcagc gacagtacgt
     3601 cgcccatgcc gagaatacgc gacgcgatgc ggtccggatg gaacggctcc agcgcctcag
     3661 ttttctcgcc aacaccgagg aacttgatcg gtttgccagt gatgtgacga atagagagcg
     3721 ccgcaccgcc gcgggcatcg ccgtccactt tggtcaacac tacgccggta agcggtaacg
     3781 cttcattgaa tgcttttgcc gtattggccg catcctgacc ggtcatggcg tcaaccacaa
     3841 acagggtttc aaccgggtta atcgacgcat ggacttgttt gatctcgtcc atcatcgctt
     3901 cgtcaacgtg cagacgacca gcggtatcca ccagcagcac gtcgtagaat ttcagtttgg
     3961 cttctttcag cgccgcgtta acgatatcta ccggcttctg accaacatca gaagggaaga
     4021 aatcaacgcc cacctgctct gccagcgtct caagctgttt gattgccgcc gggcgataaa
     4081 cgtcggcaga aaccaccagc actttcttct tgtgcttctc gcgcaggaac ttaccgagtt
     4141 taccaacgct ggttgtttta ccggcacctt gcaggcccgc catcagcacg accgcaggcg
     4201 gttgcgccgc caggttcagg gtctggttct cttcgcccat cgccgcaacc agttcgttac
     4261 ggacgatttt gacgaactcc tgccccggcg tcaggctctt attaacttca tgaccaaccg
     4321 ctttctcttt tacgcgattg ataaactcac gcactaccgg cagagcgaca tccgcctcca
     4381 gcagcgccat gcgcacttcg cgcagcgtat cttttacgtt gtcttcagtt aggcgtccac
     4441 ggccactgat attgcgcagc gtgcgcgaca aacgatcggt taaattatca aacattgtct
     4501 ctcgcctggg gtggaaacgg ttggccgcaa tcgcgacaca tcatcagtat tttgccgcag
     4561 tataacatga aggcgtcttt gttgttatgc aacggttgga gcagcgttca cctgacgcta
     4621 tactgcttct ctttcttatt gctcaaactg ttgacatcac tatgcccgtt tttgctctgc
     4681 tcgcgcttgt cgcctactcc gtcagtcttg cgctgattgt tcccggtctg ctgcaaaaaa
     4741 acggcggctg gcggcgcatg gctattattt ctgcggtcat tgcgctggtc tgccacgcaa
     4801 tcgctctgga agcccgcatc ctgcccgacg gtgatagcgg acaaaacctc agcctgctga
     4861 acgttggttc attggtcagt ttgatgatct gtacggtaat gaccattgtg gcttctcgca
     4921 atcgtggctg gttgctgcta cccattgtct atgcctttgc gcttatcaac ctggcgctgg
     4981 cgaccttcat gcccaatgaa tacatcaccc atctggaagc aacgcctggg atgctggtgc
     5041 acattggctt atcgctcttt tcctatgcca cgctaattat cgccgccctg tacgcgctgc
     5101 aactggcgtg gattgattac caactgaaga acaagaagct ggcatttaac caggaaatgc
     5161 cgccattgat gagtatcgag cgtaaaatgt tccacatcac gcagattggc gtggtgctgc
     5221 taacgctcac gctttgcact ggcctgttct acatgcacaa cctatttagc atggaaaata
     5281 tcgacaaggc tgtgctctct atcgtggcgt ggtttgtcta tattgtgctg ctgtggggac
     5341 attaccatga aggatggcgt ggacgccgcg tcgtctggtt taacgttgcg ggcgcggtaa
     5401 ttctgacact ggcctacttc ggcagccgaa ttgtccagca gttaatcagc taaacccaga
     5461 aaaggagttt cccctggaac acatttctac tactacgttg atcattattc tgatcatcat
     5521 ggtggtcatt tcagcctatt tttccgggtc cgaaaccgga atgatgaccc tcaaccgcta
     5581 tcgtctgcga catatggcga aacagggtaa tcgctcggcc aaacgcgtcg aaaaattgct
     5641 gcgtaagcca gaccgcctga taagcctggt gttaatcggc aataacctgg tcaatattct
     5701 tgcctccgcg ctcggcacta ttgttgggat gcgtttgtac ggcgatgcgg gcgtggcaat
     5761 tgcgactggt gtgctgactt ttgtcgtact ggtatttgct gaggtattgc cgaaaaccat
     5821 tgccgcgctg tacccggaaa aagtcgctta tccgagtagt tttctgctgg ctccgctgca
     5881 aattttgatg atgccgctgg tctggttgct gaatgctatc acccgtatgc tgatgcgcat
     5941 gatgggtatc aaaaccgata tcgtggttag cggctctttg agcaaagaag agttgcgcac
     6001 tatcgtgcac gaatcacgct cacaaatttc ccgtcgcaat caggatatgc tgctgtcggt
     6061 gctcgatctg gaaaaaatga ccgttgatga catcatggtg ccgcgcagtg aaattatcgg
     6121 tattgatatc aacgatgact ggaaatcgat tctgcgccaa ctctcccact cacctcacgg
     6181 gcgcatcgtg ctctaccgtg attcgctgga cgacgccatc agtatgctgc gagtacgtga
     6241 agcctggcga ttgatgtcgg agaaaaaaga gttcaccaaa gaaaccatgc tgcgcgccgc
     6301 ggacgagatc tattttgtgc cggaaggtac gccgctcagc acgcagttgg taaagtttca
     6361 gcgcaacaaa aagaaagtcg gcctggtcgt caacgagtat ggagacattc aggggctggt
     6421 gacggttgaa gatattctgg aagagattgt cggcgatttc actacgtcga tgtcgccaac
     6481 acttgccgaa gaggtcacgc cgcaaaacga cggttcggtg attatcgatg gcaccgccaa
     6541 cgtgcgggaa atcaacaaag cctttaactg gcatctaccg gaagatgatg cccgcacggt
     6601 taatggcgtc attcttgagg cactggaaga gatccctgtc gcaggcaccc gcgtgcgtat
     6661 tggcgagtac gatatagata ttctcgacgt acaggacaat atgattaagc aggtaaaagt
     6721 ttttcctgtg aaaccgctgc gcgagagtgt ggcggagtaa cgaaaacggc cctgcattcg
     6781 catgcagggc cgtgaattat tacgaaaaca gaaattacgc ttttgctttc gctacagtta
     6841 ccatcgccgc acgaatcgta cgaccattca gcgtataacc cttctgcata atgcccagta
     6901 cgttacctgg cgcaacgtca tcagattcca ccattgcgat agcctgatgc acattcgggt
     6961 ccagcggcac gttagtttcg gcgatcactt caacaccaaa cttacgcaca acatccagca
     7021 tcgacttcag cgtcagctca atgccttcaa ccatcgcaga catatccggg ttagctttat
     7081 cagccacttc cagcgcacga tccaggctat caatcaccgg cagcaattcg ttgatgaatt
     7141 tctccagcgc aaatttgtgg gctttttcaa tatccagttc agtacgtcga cgcaggtttt
     7201 ccatttcggc ttttacacgc aaaatgccgt cacgttcacg ggtctgggct tcagccagct
     7261 gagcttcgag attcgcaatt ttttcatcgc gcggatccac ctgctcagca gaagcttctg
     7321 gctcaactgc ctcaatctct tcgtgctgat ccatgataat ttcttccggg gcttgcccct
     7381 caggcgtttt ctgttcttta ctactcatga atttctccgc gtttttttcg cattcatctc
     7441 gctaacttcg cttattatgg ggatcagttt cagggtttca agggaagcac tcacattgtc
     7501 atcaatcttc gcaacaagga cctcggaaaa atgaataatc atttcaagtg tattggcatt
     7561 gtgggacacc cacggcaccc cactgcactg acaacacatg aaatgctcta ccgctggctg
     7621 tgcaaaaaag gttacgaggt catcgttgag caacaaatcg ctcacgaact gcaactgaag
     7681 aatgtgaaaa ctggcacgct cgcggagatt gggcaactag ctgatctcgc ggtagtcgtt
     7741 ggtggcgacg gtaatatgct gggcgcggca cgcacactcg cccgttacga tattaaagtt
     7801 attggaatca accgtggcaa cctgggtttc ctgactgacc ttgaccccga taacgcccag
     7861 caacagttag ccgatgtact ggaaggccac tacatcagcg agaaacgttt tttgctggaa
     7921 gcgcaagtct gtcagcaaga ttgccagaaa cgcatcagca ccgcgataaa tgaagtggtg
     7981 cttcatccag gcaaagtggc acatatgatt gagttcgaag tgtatatcga cgagatcttt
     8041 gcgttttctc agcgatctga tggactaatt atttcgacgc caacaggctc caccgcctat
     8101 tccctctctg caggcggtcc tattctgacc ccctctctgg atgcgattac cctggtgccc
     8161 atgttcccgc atacgttgtc agcacgacca ctggtcataa acagcagcag cacgatccgt
     8221 ctgcgttttt cgcatcgccg taacgacctg gaaatcagtt gcgacagcca gatagcactg
     8281 ccgattcagg aaggtgaaga tgtcctgatt cgtcgctgtg attaccatct gaatctgatt
     8341 catccgaaag attacagtta tttcaacaca ttaagcacca agctcggctg gtcaaaaaaa
     8401 ttattctaat tttacgccag cctctttact gtatataaaa ccagtttata ctgtacgcaa
     8461 taacagtaat ggtttttcat acaggaaaac gactatgttg gcacaactga ccatcagcaa
     8521 ctttgctatc gttcgtgagc ttgagattga ttttcatagc ggcatgaccg taataactgg
     8581 cgagaccggc gcgggtaaat ctattgcaat agatgccctc ggtctttgtc tcggtggtcg
     8641 cgctgaagcc gacatggtgc gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc
     8701 tctgaaagat acgccagcgg ctctgcgctg gctggaagaa aatcagcttg aagacgggca
     8761 tgaatgtttg cttcgtcgcg taatcagcag cgatggtcgc tcccgtggtt tcatcaacgg
     8821 tacagctgtt cctctgtcac aactgcgcga actgggtcag ttgctgattc agatccatgg
     8881 tcagcacgct catcaattac tcaccaaacc tgagcaccaa aaattcctgc ttgatggcta
     8941 tgccaatgaa acctctctac tgcaggaaat gaccgcacgt tatcagttgt ggcatcaaag
     9001 ctgccgtgac ctcgcgcatc atcaacagtt aagtcaggaa cgcgccgccc gtgcggaact
     9061 gctgcaatac caattaaaag aacttaacga atttaatccg cagcccggag agtttgaaca
     9121 aatcgacgaa gagtacaaac gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa
     9181 tgcattggca ttaatggcct acggtgaaga cgcaaacctg caaagtcagc tttacacggc
     9241 taaacaactg gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg tacttgatat
     9301 gctggaagaa gctaccatcc agattgctga agccagcgat gaactgcgcc actactgcga
     9361 tcgtctggat ctcgatccca accgactatt tgaacttgaa cagcgcatct caaaacagat
     9421 ttcgctggca cgtaaacatc acgtcagccc tgaggcattg ccacagtatt accagtcgct
     9481 actggaagaa cagcagcaac tggacgatca ggccgactca caagaaacgc ttgcgctggc
     9541 ggtaacgaaa catcatcagc aggcactgga aatcgcgcgc gcattacacc aacaacgcca
     9601 gcaatatgca gaagaacttg cacagctgat caccgacagt atgcatgcgc tctcaatgcc
     9661 gcatgggcag tttacgatcg atgttaaatt tgacgagcat cacctgggcg ctgacggtgc
     9721 cgatcgtatt gagtttcggg taaccaccaa cccaggtcag ccaatgcagc ctattgccaa
     9781 agtcgcatcc ggtggtgaat tgtcccgcat cgcactggca acccaggtca tcacggcgcg
     9841 taaaatggaa accccggcac tgatttttga tgaagtggat gtagggatta gcggtccaac
     9901 agcggcagtt gtcggcaaac tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt
     9961 tacccacctg ccacaagtcg cgggatgtgg tcatcaacac tattttgtca gcaaagaaac
    10021 cgatggtgcg atgacagaaa cgcatatgca atccctgaat aaaaaagcgc ggttacaaga
    10081 gctggcgcgc ctgcttggtg gcagtgaagt cacacgtaat acactggcga atgcgaaaga
    10141 actgcttgca gcgtaaactt ttttcctgct tcacggtcag agtaaacagc aaaacgccgt
    10201 aagaccggaa agcaaaaggt tttaaagtga tgaaaggtct attatcatcg gcatattaca
    10261 gatgagccac gtactgctag ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc
    10321 tgactgctgc agcagcagta ctattgatgt tgaccgcagg ctgttccact ctggagcgag
    10381 tggtttaccg tcctgacatc aaccagggga actatctgac cgctaacgac gtatccaaaa
    10441 tacgtgttgg catgacgcaa caacaagttg cgtacgcatt gggtacaccg ctgatgtccg
    10501 atccatttgg tacgaatacc tggttctatg tcttccgcca gcaaccaggt catgaaggtg
    10561 taacgcagca aacgctgacg ctgaccttta acagtagcgg tgtgttgacc aatattgata
    10621 acaaacctgc gctgagtggt aactaataat aaagttgctc tcaaagacgt taaaaaaggt
    10681 gctcaatgag cacctttttt ctgtctgtta tttattcgct gatttttctg ctcgttgcct
    10741 gcgaagctct ttcggatcgg caatgagagg gcgataaatc tccacccgat cgccatcatg
    10801 cacactatcg cttagttttg ccggacggct gtaaatgccg actttgtttt tagttaaatc
    10861 gatatcggta cgcaattcca gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc
    10921 ctcctgcagc gtcacgcgct gcaggtactg cttctcaggt agcgcataag ccacctcaac
    10981 ggcaatttta cctggcactg taaacctctt tcgcacgaac cgtaaaagcc tggaccatat
    11041 tagacgccag ctctttaaac acgcgaccaa aggcgagttc aatcaactta ttggtaaact
    11101 caaagtcgag atgaaactcg atacgacacg cctcctggct cagcggcgta aacttccatc
    11161 caccaatcaa tttcttgaac ggcccatcca ccagattcat aagaatactt tggttactgg
    11221 tcaactggtt gcgggtagta aacgttttgc tgatcccagc cttagagaca tctaccgccg
    11281 cagtcatctg cccaggagtg gactccagaa tccgacttcc ggtacaaccc ggcaaaaact
    11341 gaggataaga ctgaacgtca ttcactaact gatacatttg ctccgcgctg tagggtacca
    11401 gtgcggtccg gctaatctga ggcataacta tttccatcaa caaaaatcca acaaataata
    11461 tcatttatcc tgctgtaaaa aaaacgctat cccggcgctg ggtaacatcg ggttcatgct
    11521 aagatagagc cttgtccccc gcaggattga tatggggtgt tttcgatttc agattaccga
    11581 tgattcacga cgcttatgac gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg
    11641 cttaacaagc gcgcccgtca cgaatacttt atcgaagaag agttcgaagc gggacttgcc
    11701 ctgcaaggct gggaagttaa atccctgcgc gcaggaaaag ccaatatcag cgacagctac
    11761 gtccttctgc gtgacggaga ggcatttctg tttggcgcta acatcacgcc aatggccgtg
    11821 gcctccacgc atgtggtgtg cgatcctacc cgtacccgca agttacttct caaccagcgc
    11881 gaactggact cattgtacgg tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc
    11941 ctgtactgga aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg taaaaaacag
    12001 cacgataaac gttcagatat caaagaacgc gaatggcagg tggataaagc acgtatcatg
    12061 aaaaacgccc accgttaaac ctgcactcca attattgacc agttcctcac cgcgcctccc
    12121 tctccggcgg cgcgaatgaa catcttattg gctatcacat ccgacacaaa tgttgccatc
    12181 ccattgctta atcgaataaa aatcaggcta catgggtgct aaatctttaa cgataacgcc
    12241 attgaggctg gtcatggcgc tcataaatct ggtatacttg ccgttacaca ttggggctga
    12301 ttctggattc gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt
    12361 aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc ttaataacct
    12421 gcttagagcc ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc
    12481 caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta atcaggctag
    12541 tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg taaagactga ctaagcatgt
    12601 agtaccgagg acgtaggaat ttcggacgcg ggttcaactc ccgccagctc caccaatcat
    12661 gattggacgg tataaggaca acaccaataa aaacaggagg ttagcagtct cagcaggaca
    12721 ccgaccagac ggtgaagaga cataaaagga tacgcaaagg agccgcggct cctggtgaca
    12781 tgaaagccca cagatgtggg ctttttcgtt gatggtcaga acgaccagtt cacaccagct
    12841 accccgttcc acggggattc cacaccggca ccatggctat accccacccc aagatgcccg
    12901 cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
    12961 aaatcattga cgaaattacc gtcactattc actttcaccc ggttatcatc gacaaattct
    13021 ttgcgcacag ccgccttcag ccacggctca acttccatac cgttccccag acgcatgttg
    13081 taactcagcg ttgcacccag ttcacgatat aaacctgcgg gtatcgactg atttcgattc
    13141 catgccattg gataaatgat attcagggtt atcagcggtg aacccccgtt aacgatgcat
    13201 acggcgtcag gttccagtta ccatcggtaa atcgcatccc ggtttcaatg tgaccgccca
    13261 gcccgttgct gcgataactg ccgttggtgg ctccaccgct gccaaccgtg gagtcgcgag
    13321 cttcggtacc tgctaatacc aataaattac cgccattttc cagcaacata ttggtcgcta
    13381 aattgccgga aatggaaaaa gtgccgtact ggtaagtacc gctgatttca ataccgttag
    13441 ccgtgctcgt ctggagagcg gcaccgctgt tctggacgat atctgtcgct ttgccattat
    13501 cgttaactgt cagcgtaccg ccttcattga tctttgtttt tattgcctct ccgttagctg
    13561 aaactgtttg tattccgccg tcgttaatcg ttgtctcatt cgccaccccc tcgacgattt
    13621 gttcgccgcc ggtgagcgtc gtgcctgtcg cagtggcttt tgttttgacg atctcccgtc
    13681 cgcccgtatt gacctgtgtt ttgtcagaag aggtgtctga ctccacggtt aacacgccgc
    13741 catttgccag caggatattg ttcgccgcac cctgctcgat gctgaacgcg acgccatccg
    13801 cgcgtgttcc tgtgacccgc gtcgccctgg tggttgcaac caaagcgccc tggctactct
    13861 gctgtatccc cgttgcgctg cctttctccc gcacatcgag tgtgccgccg tcattaagca
    13921 ccgagttttc agccagaccg ccctcattaa ctacctgtga acccccatta ataatggaac
    13981 cttccgctgt cccgtttgcc ataatttgtt gtaggccaga gacgatatcg gtattgattg
    14041 ccttaccata attctgaacg gtttgcgtgc caccattgat gtgtgttttc tctgttgacc
    14101 caccatcaac aatttgttca ccactttcga tatttgcctc agtggctaat ccatataccg
    14161 tttgcttgcc acctttgata tttgctttat cagaagtggc actggcatat attgtttggg
    14221 tgccagcact attgagtaca gtgccaacat cttttccata aacatccatt ttgccgttgg
    14281 cattaataat cgtatcaact gcccgggaac cagtgacgac tgttaatgag ccagcgtttt
    14341 ccagcactac atttttagct tctgaattcc tgatgtagaa agcatcacca taactgttgg
    14401 ttcctttgat aagggttccg gaagttgtgg aagcaattaa tgcgccgccg gattgttgct
    14461 caacatgctt agcctcacca ccgtcctgaa cctccagaac gccaccatta ttaagtctgg
    14521 tg
//
LOCUS       DAJVOY010000098        14294 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVOY010000098 DAJVOY010000000
VERSION     DAJVOY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.098"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..570)
                     /locus_tag="OQL90_002598"
     CDS             complement(<1..570)
                     /locus_tag="OQL90_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1369331.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAA"
     gene            complement(958..1704)
                     /gene="cutC"
                     /locus_tag="OQL90_002599"
     CDS             complement(958..1704)
                     /gene="cutC"
                     /locus_tag="OQL90_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1369332.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1718..2284)
                     /locus_tag="OQL90_002600"
     CDS             complement(1718..2284)
                     /locus_tag="OQL90_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1369333.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2500..4233
                     /gene="argS"
                     /locus_tag="OQL90_002601"
     CDS             2500..4233
                     /gene="argS"
                     /locus_tag="OQL90_002601"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1369334.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4410..4898
                     /locus_tag="OQL90_002602"
     CDS             4410..4898
                     /locus_tag="OQL90_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCS1369335.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(5018..5410)
                     /gene="flhe"
                     /locus_tag="OQL90_002603"
     CDS             complement(5018..5410)
                     /gene="flhe"
                     /locus_tag="OQL90_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCS1369336.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5410..7488)
                     /gene="flhA"
                     /locus_tag="OQL90_002604"
     CDS             complement(5410..7488)
                     /gene="flhA"
                     /locus_tag="OQL90_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCS1369337.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7481..8584)
                     /gene="flhB"
                     /locus_tag="OQL90_002605"
                     /pseudo
     CDS             complement(7481..8584)
                     /gene="flhB"
                     /locus_tag="OQL90_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8667..9311)
                     /gene="cheZ"
                     /locus_tag="OQL90_002606"
     CDS             complement(8667..9311)
                     /gene="cheZ"
                     /locus_tag="OQL90_002606"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1369338.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLAEARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9322..9711)
                     /gene="cheY"
                     /locus_tag="OQL90_002607"
     CDS             complement(9322..9711)
                     /gene="cheY"
                     /locus_tag="OQL90_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1369339.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9726..10664)
                     /locus_tag="OQL90_002608"
     CDS             complement(9726..10664)
                     /locus_tag="OQL90_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HCS1369340.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10667..11479)
                     /gene="cheR"
                     /locus_tag="OQL90_002609"
     CDS             complement(10667..11479)
                     /gene="cheR"
                     /locus_tag="OQL90_002609"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1369341.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11617..12183)
                     /gene="flhC"
                     /locus_tag="OQL90_002610"
     CDS             complement(11617..12183)
                     /gene="flhC"
                     /locus_tag="OQL90_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1369342.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(12186..12536)
                     /gene="flhD"
                     /locus_tag="OQL90_002611"
     CDS             complement(12186..12536)
                     /gene="flhD"
                     /locus_tag="OQL90_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS1369343.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13314..13742
                     /gene="uspC"
                     /locus_tag="OQL90_002612"
     CDS             13314..13742
                     /gene="uspC"
                     /locus_tag="OQL90_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCS1369344.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13749..>14294)
                     /locus_tag="OQL90_002613"
     CDS             complement(13749..>14294)
                     /locus_tag="OQL90_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCS1369345.1"
                     /translation="TQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLN
                     QHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANEL
                     TSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQ
                     IVPRSAESQQRDKVATFPKLA"
BASE COUNT         3672 a         3777 c         3335 g         3510 t
ORIGIN      
        1 agcggcctga ctttccagct catgcgccgc gttgtcatcc atttttattt ctggcggaaa
       61 atgggcaatg cctttatgac aatcgataca ggtttcgccg cccttttgtg ctttgttatg
      121 cattttctgc gcagattcac tttgcgaggc aatatccatg gcatcaaaag aatggcaact
      181 acggcacgtt gcagagtcag ttgcttttaa ttctttccat actgtttcgg ccatttcctg
      241 gcgatgagtt tcgaacttat cgtcactgtc tattttgccg ctaacaaatt catgataaat
      301 atctttagat gcttttaatt tagcaaataa ataatccatc cctgactttg gaatatggca
      361 atctgcacat tccgcacgta tccctttctg gttcgaaaag tggacagttc cctgatattc
      421 ctcaaaaggt ttactcatcg agtggcagga aaggcaaaat gctgtatccg acgttttatg
      481 taaggctttt tgcgccagca ataacccgcc gccaccaacc acaaccgcta tcagcaaaaa
      541 caataaccca atgcgttttt tccctcgcat agattcctct aaataaattc cagaacaccg
      601 aaatcattgg ataattaaat acacaacgaa ttaaccgctt gcataactcg gcacaacact
      661 gcctgaaata atcgataaag aatatgattt attacaatgt aatcattaat tgctaaggaa
      721 taaccctgtt gttatttaga atatatattt gtcagtttta gcaatttaaa ccccataatt
      781 aatgtgaata tataaaatat ttttagcaca acgctatgtt cactgtcacc cgctcaacag
      841 atcacggtta tcgtacgttt tttatactgc tcagggataa aaaaaggccc ctgtcgaaat
      901 tgcaggggcc tggtacgagc aagcatcata ttgggcgaca tgatgcaacg gtaaaaatca
      961 tttggcctga tggcgttcaa tgattccttt catttcagca accgccgccc cgtctacgac
     1021 ataacgcgaa tactcgtccg cgtgtgcatc tgatgacatg gacaatcctt gattacgata
     1081 acgcatcggt gaagcttgcc acgctcccgc ggagctatgg acttccagca ctccggcatc
     1141 gaggaagtgg tgcaggtttt ctgcacggac tcctgctccg gccataatga ttggagcatc
     1201 acgatgggca ataagttcca taatttttga taaaccttgc agtgcatctg atttttgccc
     1261 tgatgtcagc acccgggcga tgcccaattc cgcaagatta ctgagtgtat ttaaagggtt
     1321 agcgcacata tcgaaggcgc gatgaaaagt cactgccagc ggaccggcag cagccattat
     1381 tttttccatt cgtggcatat cgacattccc gtcaacatcg agcacgcccg tcaccagtcc
     1441 aggaaaacct aattcgcgga ctgtgcgcac atcctcaaga atggcggcaa actcaccgtc
     1501 gctgtagcaa aaatcaccac cgcgtgggcg gataatcgga tgcacaggga tcgtcaccca
     1561 ctggcgcacg gatttcagta cacccaacga cggcgttaag cccccctctt ttggggctgc
     1621 gcataattca actctgtctg cgccgttttg ctgcgccgtt agtgcacatt ccatgctgta
     1681 acagcaaatt tccagtaatg ccatttttac tccttaatta cgccgactgc tcgctggcaa
     1741 cgatctcttc aatggaccac ggatgaaact taatagttgt tttgccatca gtgaccgcca
     1801 gcgtcggatt cggtaatcgc tcatactcac cttttggcga actggttttc actgagatcc
     1861 ccggtaggct gagtccttca tcagaaagca atgccagcgc acgggcattc agcgtttctg
     1921 gatcacccgg cagaacgatt tcaatatgtt cccaaccttc gtgtgggtaa cgtttttccc
     1981 cgggccacgg tagctccaca atggaaaact gccagtgcgc aacctgtacc ggttcatgca
     2041 atttaaacag acaaatcggt ctgccattga tcatattttc tgacaaaagc tcgccacact
     2101 gttcaaaacc gcgacgccag cgctcagcgg tgacgttttg atggcaacgc aaagaaatgt
     2161 gatcggcagt cagcggagtg atatccaacc ccagacggcg ggagagttca tctaatgcgt
     2221 gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc gtcgatagat tgccagttcg
     2281 ccataatcac tcttcgtctt tcagtaaaag cgttaattta ccctgttgcc ctgcgccaac
     2341 caaccgctga tttcacgccg cttctgatgc aatagtgaaa acggcaatac gccacgcgca
     2401 cgttgctgac gaaaacagcc atttgcagta tactcccgcc ctaatttctt taactggtgc
     2461 gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg tgaatattca ggctcttctc
     2521 tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc ctgcggattg cgaaccgcag
     2581 gttcgtcagt cagcaaaagt tcagttcggc gactatcagg ctaacggcat gatggcagtt
     2641 gctaaaaaac tgggtatggc accgcgacaa ttagcagagc aggtgctgac tcatctggat
     2701 cttaacggta tcgccagcaa agttgagatc gccggtccag gctttatcaa cattttcctt
     2761 gatccggcat tcctggctga acatgttcag caggcgctgg cgtccgatcg tctcggtgtt
     2821 tctacgccag aaaaacagac cattgtggtt gactactctg cgccaaacgt ggcgaaagag
     2881 atgcatgtcg gtcacctgcg ctctaccatt attggtgacg cagcagtgcg tactctggag
     2941 ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg actggggcac tcagttcggt
     3001 atgctgattg catggctgga aaagcagcag caggaaaacg ccggtgaaat ggagctggct
     3061 gaccttgaag gtttctaccg cgatgcgaaa aagcattacg acgaagatga agagtttgcc
     3121 gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg acgaatattt ccgcgagatg
     3181 tggcgcaaac tggtcgacat caccatgacg cagaaccaga tcacctacga tcgtctcaac
     3241 gtgacgctga cccgtgatga cgtgatgggc gaaagcctct acaacccaat gctgccagga
     3301 attgtggcgg atctcaaagc caaaggtctg gcagtagaaa gcgaaggggc gaccgtcgta
     3361 ttccttgatg agtttaaaaa caaggaaggc gaaccgatgg gcgtgatcat tcagaagaaa
     3421 gatggcggct atctctacac caccactgat atcgcctgtg cgaaatatcg ttatgaaaca
     3481 ctgcatgccg atcgcgtgct gtattacatc gactcccgtc agcatcaaca cctgatgcag
     3541 gcatgggcga tcgtccgtaa agcaggctat gtaccggaat ccgtaccgct ggaacaccac
     3601 atgttcggca tgatgctggg taaagacggc aaaccgttca aaacccgcgc gggtggtaca
     3661 gtgaaactgg ccgatctgct ggatgaagcc ttggaacgtg cacgccgtct ggtggcagaa
     3721 aagaacccgg atatgccagc tgacgagctg gaaaaactgg ctaacgcggt tggtattggt
     3781 gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg actacatctt cgactgggac
     3841 aacatgctgg cgtttgaggg taataccgcg ccatacatgc agtatgctta cacgcgtgta
     3901 ttgtccgtgt tccgtaaggc agaaattaac gaagagcaac tggctgcagc tccggtaatc
     3961 atccgtgaag atcgtgaagc gcaactggca gctcgcctgc tgcagtttga agaaaccctc
     4021 accgtggttg cccgtgaagg cacgccgcat gtgatgtgtg cttacctgta cgatctggct
     4081 ggtctgttct ctggcttcta cgagcactgc ccgatcctca gcgcagaaaa cgaagaagtg
     4141 cgtaacagcc gtctgaaact ggcacaactg acagcgaaga cgctaaagct gggtctggat
     4201 acgctgggta ttgagacagt agagcgtatg taatcgattt ttcgtgagag taaagcctga
     4261 tcagggttac ctgatcaggc ttttttattg ccatctaaat gtattctgaa aatggacatg
     4321 ccattgtttt ctgactgttg gataagaggc cagaagcgta atatccggcc ccagggaaac
     4381 gataacggtt gaatttaagg aataccgcag tgtttaaatt tcttgtatta acattaggca
     4441 ttatctcttg ccaggcttac gcagaagata cggttatagt aaacgaccat gacatttcag
     4501 ccatcaaaga ttgttggcaa aaaaattcag atgatgatac tgacattaac gtgatcaaat
     4561 catgcctgcg acaagaatac aatctcgtcg atgcgcaatt aaataaagcc tatggtgaag
     4621 cttatcgtta tatagaacaa gtgccacgca caggtgtaaa aaaacctgat atcgaacaac
     4681 ttaacttgct taaaaaatca cagcgagcct ggctggattt tagggacaaa gaatgtgaat
     4741 taatcctttc aaatgaggac gttcaggatt taagtgaccc ttattctgaa tcagaatggc
     4801 tctcatgtat gatcatacag accaatacgc gaactcgcca gttgcagcta taccgtaact
     4861 ctgaagattt ttatccaagc cctttgacaa gaggataatt cacatctttt tggcatgttt
     4921 tgttgcaagc tattcctgat aaataattgc aacaagacat cgagcctttt tcactgagtt
     4981 attaaacata ctcgcgagcg cgtaattttt ttgtccttca gcgataattc acaatcactt
     5041 cattacgttg tacctttagc ggtggaatta accgcccacc gcctggcact tcccagataa
     5101 atcgcaacgg ttctgctgcc gctataccgg aaaaggccac ggtggttccg ctctgcccct
     5161 ctaattcgac acaacgagat tgcgaacaca agcgaacccg cagtcctgaa ggtgtcgggc
     5221 cgataagctg ataacgccac gctaccagcg tcatcaatcc tgaagcaggt tgtcgcgtag
     5281 aaagaggcgc agacgacatc gactcaccgc gatgatttag cgtaattcct acactacttg
     5341 cctgccacat cccctccccg gcggcttgca ccagcagcgg aaacaataat attgctaata
     5401 aggttctcat tatttgccgc caattgtcgc cgtcatgcgg atatgtcggt tatcagacag
     5461 ttccagattc gacaggacca ctaactgcgg caagctgcgg cgcaggaagc gagacaataa
     5521 tggtcgcagc gcgtggttca ccaacaatac tggcggcgca cccagcatct cctgacggga
     5581 tagcgcttcc tgagtttgcg ccagtaaacg atccgccagc cctggctcca gtcctccccc
     5641 gccctgcagc gcctgtagta acaaacgttc cagcggtgta tcgaggccaa taacatggac
     5701 ttcatctttg ccaggaaacc actgctgggt aatcgcccgt cccaacgcca cgcgcacgac
     5761 ggcggttaat tcatgtggat cgctttggat gggcgcatgt tccgccagcg tttcgagaat
     5821 ggtgcgcata tcgcgaatcg gtactttttc atcgaggaga ttttgcagca ctttatgcag
     5881 tgtggtgagc gtgacgacgc caggaacgag atcttccgtc agctttggca tctcctgggc
     5941 gacgcgatcc aacagctgtt gcgcctcctg acgaccaaac agctctgcgg catgctggct
     6001 aatgaggtgg ttaagatgcg ttgctaccac cgtgctggcc tcaaccactg tgtacccctg
     6061 aatctgcgcc tgttctttta gcgcactttc aatccagata gcattcaggc caaatgccgg
     6121 atcgacggtc gcctcaccag gtaacgtccc ggcagcggtt ccagggttaa tcgccaacca
     6181 gcgccccgga taagcatcac cactgccaat ctccacgcct ttcatcaaaa tgcgatagcg
     6241 ggcaggttgc agatccatat tgtcgcgaat gtgcaccact ggcggcagaa atcccatctc
     6301 ctgggcaaat ttcttgcgga tactgcgtat acggcccaac aactcaccat cctgctggaa
     6361 atcgaccatc gggatcagtc gataacccac ttccattccc agagaatctt ccagttgtac
     6421 atcgttccac gtcgcttcga caacggtatt attctctgcc atttttaccg gtttgggttc
     6481 ggcaggcgct ttttgttcgc atccgcgtat ccaccaggcc agccccagca atccggcagt
     6541 gaacagcaaa aataccaggt tcggcattcc aggcaccaag ccgagtaaac cgagcacggc
     6601 ggcgcttaac aacataacgc ttgggttact gaaaagctga ttcaccatct gctcgccaac
     6661 atcctgatcg gtgctgacac gcgtaacgat gacccccgcg gcggtagaaa tcaccagcgc
     6721 cggaatttgt gccaccagac cgtcgccaat ggtcaatagc gtataacttt ccgccgcgtg
     6781 tcccatgctc atgccatgtt gcagcacgcc gaccagcaac ccgccgacaa cgttaatgac
     6841 catgatgagg atcccggcga tggcatcgcc gcgaacaaac ttacttgccc cgtccattga
     6901 gccgtaaaaa tcggcttcct gagtcacttc ggagcggcgt tttttcgcct catcttcacc
     6961 aatcaatcca gcgttaaggt cggcgtcaat cgccatctgc ttacccggca taccatcgag
     7021 aacaaagcgc gcacccactt ctgcgatacg cccggcacct ttggtaatga ccataaagtt
     7081 gatgatcacg agaatgacaa acaccacgat acctatagcg aaattgccac caacgaggaa
     7141 gtgaccgaac gcttcgacca ccttccctgc cgccgccgcg ccggtatgcc cttccatcaa
     7201 aatgatacgg gttgaagcca cgttaagtgc cagacgcaac aacgtagtaa acaacagaat
     7261 ggtcggaaac gcagcaaact caagcgtgcg ctgggtaaac atcgccacca gcaacaccat
     7321 gatcgacaag gcaatattga aggtaaacaa caggtcgagt atgaatgcgg gcagtggcag
     7381 caccatcatc gacaagatca acaggatcaa aatcggtccg gcaaggatct gccattgtgt
     7441 cgatttcagg tttgcgggca ggcgcagcat cgcggccaga ttactcatgg gtcggtttct
     7501 cgttaataaa atccagggct tccggcaccg gaagatgagt aggttgtaca gggcgctgtc
     7561 caccagccag acgccagcgt ttcagttgcc agatccaggc cagcacttcc gccaccgcgg
     7621 cgtacagttg acccgggatt tgttgaccaa tctccgcatg tcgatacagc gctcgcgcca
     7681 gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc aatttcacga atgcgcagcg
     7741 cgaccagccc tgcaccttta gcgaccactt tcggtgcgct cattttgttt tcgtcatact
     7801 gcaacgctac cgaatagtgg gtcgggttat tgacaatgac atccgctttc ggcacatcgg
     7861 ccatcatccg acgccgtgcg gcagctcgct gcatctgacg gatccgtcct ttaacatggg
     7921 ggtcgccttc gctttgtttg aactcatcac gaatatcctg ccgcgacatt cgcagctttt
     7981 tcaggtggct gaagatttgg aaaaagacgt caaatcccac cattggaatg acaccaagca
     8041 ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt acccatggcg gtaatcggag
     8101 actcggccag taagcgcatc atctgcggcc agtgatgcca gagaaaaaac cccgtcacgc
     8161 tgccaaccaa gatggttttc aaaattgctt taagcaactc cgcgccagtc tgtgccgaga
     8221 acatccgttt aatgcccggt agcgggttga gtttggaaaa cttcggctgc aaggattttc
     8281 cgctaaatac cagccccccc agcatgaccg gagaaatgag cgctaccagc accacgccgc
     8341 taatcagcgg tagcagcgcc agcatggctt ctctgatcag cagaataatc tgcccgagga
     8401 tcagattcgg gtctttgata atactgtgat caaaatgcag cccagcggag agcatgcccg
     8461 acaatcgacg ggccagcgac acaccgccga accagataac acacacgccc accagcaaaa
     8521 tcagcagtga ggtcagttca cgggaacgcg ggatttaggg cttttgctgc cacacatcaa
     8581 gcatagtgtg cgtttgtcgg atgcggcgtc atcgccttat cagaccgcct gatatgacgt
     8641 ggtcacgcca catcaggcaa tacaaatcaa aatccaagac tatccaacaa atcgtccacc
     8701 tgatcctgac tggctaccac accggctttg ctggtatcga cctgaggtcc attaagcaaa
     8761 ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa cagcaccatc
     8821 agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac ctgcccggtg
     8881 agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt aaagctggta
     8941 tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg ggcctcggca
     9001 aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt tgctgatttc
     9061 tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg ctccgcagcc
     9121 tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat ggcttccgcc
     9181 gcttcggcaa tggcctgatc cagccccagt tcccgcaaac tgtcgcgcag catacgcgtc
     9241 aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg tttgattgat
     9301 ggttgcatca tagtcgcatc ctcacatgtc cagtttatca aagattttgt tgagtttttc
     9361 ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg cttgcgccgc
     9421 agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca atgccgacat
     9481 cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg gcatgttcca
     9541 gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt cgacgccatc
     9601 ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca ctatgcgtcg
     9661 catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca tttcacactc
     9721 ctgatttaaa tacgtatcgc ctgtccggca ctaatttttg ccaacatttg ctggcttacc
     9781 tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg cggcatgccg
     9841 aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg acgcatcgcc
     9901 aacattccca ccgggccgtc gtgaattttg atttgataat ttgcgccact acgcgccagt
     9961 tccatatgcc gatcgcccgg cgcaatatag gcatgccccg gcaagacacg ttctccgtct
    10021 tcggcttctt taaccccgat ctggcaaagc ttattaagtc tgtcggcaaa agagcgggtg
    10081 aaaccgggcg gcatatgttg ggtaattaac aatgccgggc tggaaagcgg caacggttgc
    10141 agtacgtgac gaattgcctc agttccaccc gttgaagcac caatcgcaat cagtttttca
    10201 gaactcaaca acggacccgc cttcagcgtt gtcggtgctg acaatggctt atgtgctgca
    10261 aggctcgcct ttgctgccgt acgcaccttt tcagcaatca tttcgctata cgccagcatt
    10321 ccttcgcgaa tacccagttg cggtttggtg acaaaatcta ttgcccccag ctccagcgcg
    10381 cgcagcgtga cttctgatcc tttgccggtc aaggaagaaa ccatcacaac gggcattgga
    10441 cgcaaacgca ttaatttttc gaggaaatcc agtccgtcca tccgcggcat ttcaacatcc
    10501 agcgtcagca catcgggatt gaatttctta atcaagtcac gcgcgaccag cggatcaggc
    10561 gcggtcgcca ccatttccat gtcgctatgg ctgttgatga tttctgtcat gatctggcgc
    10621 atcagtgccg aatcatcgac agataacacc ctgattttgc tcatcgttaa tccttactta
    10681 gcgcatacac cgtctgacca cgcagcgtga agcggcgctc aaggtggcta aagttttcag
    10741 agtgacccgc aaacagcaat ccgtcgggtt taaggagcgg aacaaagcgg cgcaaaatct
    10801 cctgctgggt attttgatcg aagtagatca tgacgttacg acagaagatc gcatcaaacg
    10861 gccccggcac ggtgtactgt ttcgccagta gattcagcgg ggcaaaatca acatagttcg
    10921 ccagctcctg acgcacgcgt accagccctt catgcggtcc cgtccctcgc atgaaatacc
    10981 gttgcagttg ctgcggcgtc aggtttttca actcttcatg gcgataaata ccgcttctgg
    11041 ctttttccag cacttcggtg tcgatatcac tggcaaacac tttccagcgt ccgggcgcgg
    11101 tgcccaatgt gtcagccagc gtcatcgcaa tgctgtacgg ctcttcgccg gttgaagccg
    11161 ccgcgctcca tacgcgatac tcgccagaac ggcgacgtgc gtgatccgcg agcagaggga
    11221 aatgatgtgc ctcacggaaa aatgccgtca gattcgtggt cagcgaattg ataaacgcct
    11281 gccactcacc gctgtgctga ttagattcca gcaagttcag ataatgaccg aaatccgtca
    11341 gtcccagcga acgcaaacga cgaaccagtc ggttgtaaac catgtcgcgt ttatgatcgg
    11401 ccaggacgat cccggctcgt tgatagatca attgacttat ccgccgaaaa ctgtaccgag
    11461 aacaaccagg taacctaata agataagcac gacatcatcc ttccactgtt gaccatgaca
    11521 ggatgttcag tcgtcaggcg ttaacgcgcg attggggcaa aaaaaagcag cggtacgtcg
    11581 ttaccgctgc tggcatgttg cgccacaccg tatcagttaa acagcctgta ctctctgttc
    11641 atccagcagt tgtgggataa tatcggcagg attctgggaa agtttacgtc tttttactgc
    11701 ccgggatggc ggttgacata agctgcaggc aaagctgcca acaggctggt gagcgtgggt
    11761 aataaaattg ccgccgcagc agttgcagct ggaaagttgc agtaatccac tttcaacaaa
    11821 ccgcaccaat gtccaggcac gggttaatgc cagcagtggt ccttcttctg cttgtgggca
    11881 ctgttcaagg tataaacggt aggctttgat caccgcatcg acgccattac acaaacctgt
    11941 tttcagtaaa aactgccatg cattacagaa catcgaagca tgaacgtttt gttcccaggt
    12001 cataaaccag tcggttgaga atggcagcat gcctttcggc ggtgggcttc cgcgcagttc
    12061 tttataaagt tttatcaggc gtccgcgact taactgtgtt tcgctttcca gcatctgcaa
    12121 acgagcgccc agggtgatca attcaatatc ccgcgcttcc tgaacaatgc ttttttcact
    12181 catgatcagg cccttttctt gcgcagcgct tcttcaggct gattaacatc attcagcaag
    12241 cgtgttgaga gcatgatgcc ggtatgaatt tgctggagat cgttaacgcg ggaatcttgc
    12301 gtcaactgag taatcgtctg gtggctgtca aaacggaagt gacaaaccag ttgattggtt
    12361 tctgccagct taaccatttg cggaagagtc agtgccgcta acgttgtcgc catttcttca
    12421 tttatgccga gacgaaacat agcggacgct ttgtcctgaa caatcaaacg ctgtgcaagt
    12481 agtaaatatg acaagttgat gtcataaatg tgtttcagca actcggaggt atgcattatt
    12541 cccacccaga ataaccaact ttatttttat gcggtttcac cgcaccccgt gatgtcgccg
    12601 ggaagccccg gtaaaaaata attagcatta gaatagtgcg ataagctgca ataagcagaa
    12661 ccaccttttt ggtttaatat gtccttacaa atagaaatgg gtctttacac ttatctaaga
    12721 tttttcctaa atcgacgcaa ctgtactcgt cactacacgc acatacaacg gagggggctg
    12781 cgattttcaa taatgtgtga tgcagatcac acaaaacact caattactta acataaatgt
    12841 ataagccata cttttgtttt gggtgtattt caatctgtta aaaagttttt cgctacgcta
    12901 gcaagcaaaa atgaaactgg aataatcgaa atgggatgtt gcgcacagtt aaaataactc
    12961 accgtaaata atcatctgct ataaataatc actttcatgc aataccagat aagctatttt
    13021 taaacagaca cttaccgcac aacaaactaa ttaaatacaa agaaaggcaa caatcaactt
    13081 aatgttaatg aaatgtttta catatattaa acacttgttt aatgcggaga atcagtacac
    13141 tattttctta taattacatt tgaaattata tgatcagcgg gagtgattga ttaattgtct
    13201 ttactaataa tcagactaat atttacctgt ttgaccgagt tgggattgcg tcgtttctcc
    13261 attaggagta aagctttaat gtcacctgaa gttcacagaa taaggaacag gttatgagct
    13321 atagcaatat tcttgtcgct gttgcggtta caccggaaag tcagcaactg ctggcaaaag
    13381 cggtatctat cgccaggcca gtaaagggac acatcagttt aattactctc gcttccgacc
    13441 cggaaatgta caatcaatta gccgcgccga tgcttgaaga tttacgcagc gtgatgcaag
    13501 aagaaacgca aagctttctt gataagttaa ttcaggatgc ggggtatccc gttgacaaga
    13561 cgtttattgc ctacggtgag ttaagcgaac atattctgga agtatgtcac aaacatcatt
    13621 tcgatttggt gatttgcggt aatcacaatc acagtttctt ttcgcgagca tcctgctcgg
    13681 cgaaaagagt gattgcctca agtgaggtcg atgtgctgtt agttccactt acgggtgatt
    13741 aaccgctcct acgcaagctt tggaaaggta gcaactttat cgcgctgctg gctttccgcg
    13801 cttcgcggaa ctatctgctt taggtcgcta atgaagcact cctgccagtg gttaatatcg
    13861 tttttcacga taacgtccag catttctgca tgacgggaaa tacgttccgc cagcgacata
    13921 gtcaatgcac gatccagcgc agctgcaact tcgtcacgat cgtaggggtt aacaattaac
    13981 gccgacgtta actcgtttgc cgctcccgca aattgcgaaa gaacaagaac gcccgggttg
    14041 gctgggtcct gagcagcaac atactctttt gctaccaggt tcatcccgtc acgcagtggc
    14101 gtcactaagc ccacgtcaga gtagcggaat attttcatca gtaatttacg gtcaaaatgc
    14161 tgattcaaat aataaagcgg cgtccagcct aattgcccgt atttaccatt aattcgtcca
    14221 gcttcatttt cgagctgatg acgaatatcc tgataggctt gcacatcacc acgcgacgtt
    14281 ggtgcaatct gggt
//
LOCUS       DAJVOY010000099        13597 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVOY010000099 DAJVOY010000000
VERSION     DAJVOY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.099"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(89..775)
                     /locus_tag="OQL90_002614"
     CDS             complement(89..775)
                     /locus_tag="OQL90_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1369346.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OQL90_002615"
     CDS             complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OQL90_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1369347.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OQL90_002616"
     CDS             complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OQL90_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1369348.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OQL90_002617"
     CDS             complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OQL90_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1369349.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(5228..6056)
                     /locus_tag="OQL90_002618"
                     /pseudo
     CDS             complement(5228..6056)
                     /locus_tag="OQL90_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OQL90_002619"
     CDS             complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OQL90_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCS1369350.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(8392..8601)
                     /locus_tag="OQL90_002620"
     CDS             complement(8392..8601)
                     /locus_tag="OQL90_002620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369351.1"
                     /translation="MMLQFCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8656..12618
                     /gene="putA"
                     /locus_tag="OQL90_002621"
     CDS             8656..12618
                     /gene="putA"
                     /locus_tag="OQL90_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1369352.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12658..13296)
                     /gene="rutR"
                     /locus_tag="OQL90_002622"
     CDS             complement(12658..13296)
                     /gene="rutR"
                     /locus_tag="OQL90_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS1369353.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         3373 a         3697 c         3352 g         3175 t
ORIGIN      
        1 tttaaagaag agctcgtacg aaggtacggg ctttttttcg tatattgcac acaccggggg
       61 gatgagaagc cccgaccggg atacttagtt aatgcaccgg ttcactcttt gtcgccgcga
      121 ctaatgggcg aattttgtca cgagtctccc agaccggagg aatttcctcc aatttagtga
      181 cagaagctcg tacaaaaggc acccgtgcaa gagtcataat tgtatccaac caacggtcac
      241 gaataatgcg atccgccctt tcatgactcg ggtcatccag ctcaataaca cagactattt
      301 tattgttctt ctttgaaacc agaacataat ccattgccat gccattataa attgcaaaga
      361 aacgctttct ttcttcttcg ctaaactcag gttgttctgg aaagttgatc aagcgcccca
      421 gagagacctg gcaatggata tgacaataat gtccaaacca tttttccaga ttatcgaaat
      481 acattttttc acaacgagtg attaaccact cttttctttc caataaaata ccttcggcaa
      541 ccattccctg aaataactca tctcgctctt gccaacgaga gtcacgatag gttttctgcg
      601 gtatatagct ccttgtcgac gccttagcgg caggggaagt tgcagatgaa gggacgggtt
      661 gcgatttagc gatgattacc gatgaggcaa cttttcggag tgtcaatttt ctggcaacag
      721 caaaaccggc gataaatgca attgtcaaac aagttacaaa agccataaag ctcataatta
      781 attttcacct atccatgttg attatatttc attaccaatg ttactcgctg cagcgtgtta
      841 catgccaaca atgcaacgat caatatatag ttagtatcat gttttgatta ttacaaccat
      901 ccctatttag ccattgcggc ttgccttcca caaaaaaata acaacacaca atattagcaa
      961 agaattaatt aagattaacc ttacatttat aatattatcc ctaaagaata aatcagagga
     1021 atatattcgg tgttaaacag tcagtcaaag tatagcccat tgttgattaa acctcataag
     1081 caaaattggc accagatctc ctgtacaggt tgaatggccg aaaaaagata ggcagatgct
     1141 catttttttg gcttctggaa gaaaaactct ccgcgctggc agtattacac tggtctgatg
     1201 ctatgacgaa aaatgcctgg taagagtatc taaaccagac tccagttcag cttatcaaca
     1261 cgctcaaagt cgcccccctg aacgctaacc tgaagtgaaa atctgttgaa ataaaacaaa
     1321 gcccggttcg cccgggctct gcaccgataa cacacttaac tgtatgcatg cagcgtacgt
     1381 tggcaaagtg ccgaacgtac gcaatcctct tttccgaacc ggacgatccc aaccatttca
     1441 tcttcttcga aacgttccag cgcgtcactt aatccggagc acacgccgcg aggcaaatcg
     1501 cattgcgtga tatcaccgtt gacgataacc gtcacgttct ccccgaggcg ggttaaaaac
     1561 attttcattt gcgcggcagt cacattctgc gcctcgtcaa gaatgacgac tgcattttca
     1621 aaggtacgtc cacgcatata ggcgaacggc gcgatttcca ccttccctat ttccggtcgc
     1681 aggcagtact gcataaagga agcccctaag cgccggacca gcacgtcgta gaccgggcga
     1741 aaatagggag caaacttttc tgcgatatca ccaggtaaga agccaagatc ttcatcggct
     1801 tgcagaactg gacgggtgac gataatcctg tcgacatcct tatgtatcag ggcctctgcc
     1861 gcttttgctg cgctgatcca ggtttttccg cacccggctt cgcccgtggc gaatatcagc
     1921 tgcttactct caatagcctt cagatagtgc aattgcgctt catttcgcgc gaggatgggc
     1981 gaggtatcgc gactgtcgcg ggccatacca atggcttcta cgccgctcat ctgcacaagc
     2041 gaggtgaccg attcttcttc acgctgctta tggctgcgcg aatcccgtct cagcacacgt
     2101 tttgcctcgc gacgagcttt gatcactgct ttttgtcttc ccatggagag caccttgagt
     2161 tgtttgtatt catcacacgc gccgttggca gcgcgattat gcgcacgaac atcagagggt
     2221 tggcttcctt gtaagccata gtttgctttt ggataaaatg ccgaaaaacg gctacgcgca
     2281 ccgtttacgg cgtcggtaac acatgaaaag aaaggatgag gttgaaaatg caaagtgacg
     2341 agatgactac cggaggagaa aactccgcga gtggtggcgc gttgattatc taaaacatgt
     2401 ccagtacagg acgttaccat ccgcgatctc catagtgact gactatcact gccgggaact
     2461 tccgctgcta cttaataagt acaacagatc tcgcatttta ttgcaacaat atatttactt
     2521 atatttaact ataaaacact atttcagtga cattagtttc tactggaaag atgacagagt
     2581 gatgacagtg atgaaaaaag ctgtgtgctt tcagcaggat ttgcatcagg catcagtgca
     2641 cgatcgccgg atgcggcgtg aacgccttat ccagcctacc aaacccgcac tgattgtagg
     2701 catgataaga cgcgcaagcg tcgcatcagg catctgcgca caaatgcctt atccgcctaa
     2761 aaacattaaa cccgcaataa cgcgcttccg agataatcgt tcgcgtcctt cactcccggc
     2821 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     2881 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     2941 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3001 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3061 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3121 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3181 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3241 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3301 ttatcattct ggctatcggg attggcagtg ccgtctttaa agcccagcaa attaatcggc
     3361 gtctctttgc ctttactacg cgctgcgtga tcggaaataa acccttcccg cttccagcgc
     3421 acgctgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggat caccgtgtcc
     3481 tgggtgttgg cgcaaatttg tagcaacaca tcaccatggc ataacgccgc atccagcgag
     3541 tcgttgggga aacgcgtcat cttctgcaac tttttcggca tctgtggcgc aaggccaaac
     3601 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     3661 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgcc
     3721 gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgttcaaga
     3781 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     3841 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     3901 gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     3961 agcgccagcg cacctatcac tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt
     4021 tcatctttat actgcataac gcttaatcca gtcccagcac accgcgaagt tgcgccagat
     4081 cttccgcctg cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg gtcaatttgt
     4141 cgtaggtttc aaaaccgtct ttagtacggt atttcgccag aatggtatcg acctttttaa
     4201 agttggcatc gacttttgcc agcagttccg ggttggcttt ttgcagttgt ggacgcagca
     4261 aatcgacaat tttctgcgag ccttcaacgt tagcctggaa atcccacaga tcggtgtggc
     4321 tgtagcgatc ttcttcaccg ctaattttgc tggctgccac ttcctcaatc agtccggctg
     4381 cgccgccgac cacttttgaa ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga
     4441 ccacatctgt ataaagctgc tcagcgtact gatccatccc tttggtggtg ttgtcgccaa
     4501 acaatgcttt ttccagacgg tggaaaccag tgaattttgg gtcggcggct ttttgctcgt
     4561 aatcatcttc acgggcgtca atgctgccat ccagatcgga gaacagttca gcaatcggtt
     4621 caatgcgctc atagtgctgg cgcgtcggcg catacagtgc tttcgctttt tcgatatcgc
     4681 cagctttaat cgcgtcggta aaggctttgg tgtcggtcac cagctgcgtg gtttccgcca
     4741 tgacatacgc tttatatgca gtaattgcac caccaagact taacagcgca tcactttgcg
     4801 ccgcatccgc cgttgcctca cctttgacga tcaacttccc tttcgggtta gtcagcagac
     4861 cgcaggtcat atcgtattcg ccaggctgta aattcgccgt cattttctgg ctaaaaccag
     4921 gggcgatatt ttcccgctct tccaccacca tcacgccttt gaggatctcc cactccagcg
     4981 ccttctggct atggttctga ataatgaact gtgttttccc ggcgttaacc gtaatggtca
     5041 tcggttcgca ctgcttatcc gtcacggtca ctttgacctg cggcacatca gcggcgttag
     5101 ccataaaagc agaagaaaac agcgcagcca cgctcaactg caatgcgtta cggcagaagt
     5161 taatggtcat gacgagacta tccctttaaa gtatgttgta actaagcaag agtttgcgtc
     5221 gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg tggtggcata gcaaatgcca
     5281 ccagcgccgg gatgagataa ataaaccaga cggcgacttc gctgacgctc ggcgcttcct
     5341 gatagccaaa aatcccttcc atcagcgtgc caaacagcga gtgagttgag agcaccgcac
     5401 tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa
     5461 tggcaccagc tgccagccct gcggcgacga agagaataaa caggctggtc catttaaaaa
     5521 atgcaccaag attgaggcga ataccgcccc agtagagcag aaagccgagc accacggctg
     5581 tagcaagacc gagcattgca cccagcggcg gccagatccc gacatcttgt tgaaatgccg
     5641 ccagcaggaa aaagaccgac tccagccctt cccttgcaac ggcaaaaaag accatcatca
     5701 ccagcgccca gccatgatga tttccacgct acaatgcgct atcgactgcc tgttccagtt
     5761 gcacttacgt tgcgcgacac tttgcgcatc cagaaaacca tccaggtaag gatcaccacg
     5821 gcgatcaccg ccacgatacc ttcaaacagt tcctgttctt tttgcggaaa ttcgccggtg
     5881 gtttcgttaa tgaagatccc caggcccagg cacaacgcag cggcaagcaa cacgccaatc
     5941 cacatcacac caatccatcg gcctcgctgg gtacgcttaa gatagctggc aatcaaactg
     6001 acaatcagcg cggcttcaag tccttcgcgc aacataatga gaaacggaac aaacatgcca
     6061 cccaccctta aacgtttttc tgagtcaatc tatgcaaaga aaagtaaatc gcagtgatag
     6121 cgattatcat tacggtaaag aaaaatacaa gcggaatgtt tataaaatga tgttggaatg
     6181 taaacaaaga gaggtaatgc caggggatat aaacgattat tccagggtag aaagatggga
     6241 ttgtttgcaa agaaccatgt gttcttgccg gatgccgcgt aaacgccata tccagccgac
     6301 aaaactgtcc agactcaatg catcagagct tccatgtaaa actgataagc gggtgtatca
     6361 agcaatttcg tccatccgcg attatcaaat ctaacttttt gcccggaaca taataacagc
     6421 tcgggtgttg gtggcgtcaa aatcgctgaa gcgttccccg aaggccggga cagcccacat
     6481 ggatgtgggc tgagggcgcg tcttacagga cgttacctcg cgcccgaccc ggtagccgaa
     6541 ggggataagc tgaaggtacc gcgcagcggc gattttgttc gccagagccc gggggtgcac
     6601 agggggcggc ggcgattggc cgccccctgc gcgctccctg caccattcaa tttatatggc
     6661 gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc gcaggctaag
     6721 tcccttagct ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca tcggcctcgg
     6781 caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac actacaatcc
     6841 cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag ccgaactgct
     6901 tccagacgat aaccgtcagc gcaccgatga tcatccccgc cagtgcaccg ttacgcgtca
     6961 tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca aagcctgccc
     7021 acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc gcaatcgcca
     7081 ccagcgccac caccagcacc atcacacgcc ctacccacac cagctctttc tggctggcat
     7141 gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac accagcagtt
     7201 ggcaacttaa ggttgacatt accgccgcca gaattgccga cagcagaatc ccggcaatcc
     7261 acgggttaaa cagaatttgc gccagttcga taaacacgcg ctcggcgttc tggtttaccg
     7321 cgcctgctac cgccgggtgc tcattaaagt aagcaatccc aaagaagccg acagccaccg
     7381 cccctgccag gcagaggatc atccaggtca tactgatacg acgcgcatgg acaatgctgt
     7441 ggtgagaatc cgccgccata aaacgcgcca gaatgtgcgg ctgcccgaag taacccagcc
     7501 cccaacccat cagtgagata atggcaacaa agttcagacc tttgagcata tcaacgtttt
     7561 cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca ctgataatga
     7621 cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca gtgtcagtcc
     7681 agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgccgca gctcccgccc
     7741 acagagccgt ttcgtagctc atgccaaagg tactttcaaa cagacgcgcg cctgccacaa
     7801 tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag ataatgcgca
     7861 aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt aaggcgttat
     7921 tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg atccacgcgc
     7981 ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa accgcgccag
     8041 gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat gccgtcacaa
     8101 atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt gatcgccagg
     8161 cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc aacatcggtg
     8221 tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa acttgccgtt
     8281 atatctgcaa ccggaacggg gtaacagagt ttatttttta ccagggcgac cgtatcctgc
     8341 cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat tttagtgctc
     8401 agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg cacctttcaa
     8461 aaatgttaac tgccgcagag aaaaagtctg agttattttt ttaatccctg tcatatcaat
     8521 ttcttttatt aacatttcat tcatttttaa gcttgctacg caggtcacat ttaacatggt
     8581 tgcacaaaat tgcaacatca tggatatttc acgataacgt taagttgcac ctttcagaac
     8641 aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga cgcgacgcgt
     8701 gagcgtatta agtctgccgc gacacgtatc gatcgcacgc cacactggtt aattaagcag
     8761 gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccaga gctacctgcg
     8821 ctgctttctg gtgctgccaa tgagagcgat gaagcaccga ctccggcaga ggaaccacac
     8881 cagccattcc tcgactttgc cgagcaaatt ttgccccagt cggtttcccg cgccgcgatc
     8941 accgcggcct atcgtcgccc ggaaaccgaa gcggtttcta tgctgctgga acaagcccgc
     9001 ctgccgcagc cagttgctga acaggcgcat aaactggcat atcaactggc cgataaactg
     9061 cgtaatcaaa aaaatgccag tggtcgcgca ggtatggtcc aagggttatt gcaggagttt
     9121 tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctgg cggaagcgtt gttgcgtatt
     9181 cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa tcagcaactg taactggcag
     9241 tcacacattg gtcgtagccc gtcactgttt gttaatgccg ctacctgggg gctgttgttt
     9301 actggcaaac tggtttccac ccataacgaa gccagcctct cccgctcgct gaaccgcatt
     9361 atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat gcgcctgatg
     9421 ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt tagccaatgc ccgcaagctg
     9481 gaagagaaag gtttccgtta ctcttacgat atgctgggcg aagctgcact gactgccgca
     9541 gacgcacagg cgtatatggt ttcctatcag caggcgattc acgccattgg taaagcgtct
     9601 aacggtcgtg gcatctatga aggtccgggc atttcaatca agctgtcggc gctgcatccg
     9661 cgctacagcc gcgcccagta tgaccgggta atggaagagc tttacccgcg tctgaaatca
     9721 ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca tcgatgccga agaggccgat
     9781 cgtctggaga tctccctcga tctgctggaa aatctctgtt tcgagccgga actggcaggc
     9841 tggaacggca tcggttttgt tattcaggct tatcaaaaac gctgcccgtt ggtgatcgat
     9901 tacctggttg atctcgccac ccgcagccgt cgccgtctga tgattcgcct ggtgaaaggc
     9961 gcgtactggg atagtgaaat caagcgtgcg cagatggacg gccttgaagg ctatccggtt
    10021 tatacccgca aggtgtatac cgacgtttct tatctcgcct gtgcgaaaaa gctgctggcg
    10081 gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct ggcagcgatt
    10141 tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg cctgcatggt
    10201 atgggcgagc cactgtacga gcaggtcaca gggaaagttg ccgacggtaa acttaaccga
    10261 ccgtgtcgta tttatgcccc ggttggcaca tatgaaacgc tgctggcgta tctggtgcgt
    10321 cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga cacctctttg
    10381 ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc gcaacaggaa
    10441 gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta cggtcacggg
    10501 cgcgacaact cggcagggct ggatctcgct aacgaacacc gtctggcctc gctctcctct
    10561 gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga acaaccggta
    10621 gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat tgtgggcttt
    10681 gtgcgtgaag ccacgccgcg tgaagtagaa caggcgctgg aaagtgcggt taataacgcg
    10741 ccaatctggt ttgccacgcc tccggctgaa cgcgcggcga ttttgcatcg cgctgccgtg
    10801 ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc cggaaaaacc
    10861 ttcagtaacg ctattgccga agtgcgcgaa gcggtcgatt ttctccacta ctacgccgga
    10921 caggtgcggg atgatttcgc taacgaaacc caccgtccat tagggcctgt ggtgtgtatc
    10981 agtccgtgga acttcccgct ggctattttc accgggcaga tcgctgccgc actggcggca
    11041 ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc gcaagggatc
    11101 gccattttgc tggaagcggg tgtaccccct ggcgtggtgc aattgctgcc aggtcagggg
    11161 gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtgc gcggggtgat gtttaccggt
    11221 tcaaccgaag tcgctacgtt actgcagtgc aatatcgcca gccgcctgga cgctcagggt
    11281 cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt cgattcttca
    11341 gcactgaccg aacaagtcgt cgtggatgtg ctggcctcgg cgttcgacag tgcgggtcag
    11401 cgttgttcgg cactgcgcgt gctgtgcctg caagatgaga ttgccgacca cacgttgaaa
    11461 atgctgcgcg gtgcaatggc cgaatgccgg atgggtaatc cgggtcgcct gaccaccgat
    11521 atcggtccgg tgattgatag cgaagcgaaa gccaatattg agcgccatat tcagaccatg
    11581 cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagcgaaga tgcccgtgaa
    11641 tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt tgccgaattg
    11701 cacaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa ccagctacca
    11761 gagctgatcg agcagattaa cgcttccggc tatggtctga cgcttggtgt ccatacgcgt
    11821 attgatgaaa ccatcgccca ggtcactggc tcggcccatg ttggtaacct gtatgttaac
    11881 cgtaatatgg tgggcgcagt ggttggtgtg cagccgttcg gcggcgaagg gttgtccggt
    11941 accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa tcgcccggaa
    12001 agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt atccggtcga tgcacagttg
    12061 aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa tcgtccagaa
    12121 ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaataca acgattgctg
    12181 ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg cgtgttgtgt
    12241 attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc ggtgggcagc
    12301 caggtactgt ggccggatga cgcgctgcat cgtcagctag taaaggcatt gccatcggca
    12361 gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc ctttgatgcg
    12421 gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt tgctgcgcgg
    12481 gatggagcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat ccttctggaa
    12541 cggctgtata tcgaacgttc gctgagtgtg aataccgctg ccgctggcgg taacgccagt
    12601 ttaatgacga tcggttaaac aggtagccgg aggatgttac accctcctcc ggcatcttta
    12661 acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt gattgaaaaa
    12721 tacctcatcg cgcaacgttg cgcctgtcac cgcctccacc tgaggggcga aatcggcgta
    12781 atgttgagtg gaagcccaaa tcataaaaat caaatgctgc ggatcaatcg gcgcgagttt
    12841 gccgcttttg acccaaccgg caatcagcgc cgatttctca tcaattaatg ccttcaaatc
    12901 gcccgtcagt tcatccatta acagcggcgc gcctgccagc atctccatac agaacaggcg
    12961 cgaagcctgc ggataatcgc gtgagacttc cagcttcaga cggatgtact ctttgatcgc
    13021 cgccagcggg gcgaaatctt cacgaaacgc ttttaacggt gccagccaga tatcgagaat
    13081 ctgccgcagc acggcaatat acagcgcctc ttttgacgga aagtaataca gcagattggt
    13141 ttttgaaaca cccgccaact ctgcgatctg ctccagcctt gtgccgtgaa aaccgaattg
    13201 tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc ttcgcgctta ctgcgcgcga
    13261 acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc tctcccctct tgtagattgg
    13321 caacaggata gcaaaacccc cgcccggcct cgaccttctg cactctcatc gcgcacggat
    13381 gcatgtttta tgtgcaactg ttttgaccgt ttagtccact ttttaccaga tattttagat
    13441 aacacaatgc taacacatta ataacattca ctttttaaaa actggcatcc gctttgcaaa
    13501 caagccaata cgcggctgaa aaggatatgc aggatgcagc gccccgtctg actttcactt
    13561 tacgagacga agagaggttg atgatgaaaa ttggcgt
//
LOCUS       DAJVOY010000100        13564 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVOY010000100 DAJVOY010000000
VERSION     DAJVOY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.100"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(231..1604)
                     /gene="cobA"
                     /locus_tag="OQL90_002623"
     CDS             complement(231..1604)
                     /gene="cobA"
                     /locus_tag="OQL90_002623"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1369354.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1623..2429)
                     /gene="nirC"
                     /locus_tag="OQL90_002624"
     CDS             complement(1623..2429)
                     /gene="nirC"
                     /locus_tag="OQL90_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1369355.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2556..2882)
                     /gene="nirD"
                     /locus_tag="OQL90_002625"
     CDS             complement(2556..2882)
                     /gene="nirD"
                     /locus_tag="OQL90_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1369356.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2879..5422)
                     /gene="nirB"
                     /locus_tag="OQL90_002626"
     CDS             complement(2879..5422)
                     /gene="nirB"
                     /locus_tag="OQL90_002626"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1369357.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5684..6865)
                     /gene="tsgA"
                     /locus_tag="OQL90_002627"
     CDS             complement(5684..6865)
                     /gene="tsgA"
                     /locus_tag="OQL90_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1369358.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7136..7708
                     /gene="ppiA"
                     /locus_tag="OQL90_002628"
                     /gene_synonym="rotA"
     CDS             7136..7708
                     /gene="ppiA"
                     /locus_tag="OQL90_002628"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1369359.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7813..7980
                     /locus_tag="OQL90_002629"
     CDS             7813..7980
                     /locus_tag="OQL90_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1369360.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7970..8572
                     /locus_tag="OQL90_002630"
     CDS             7970..8572
                     /locus_tag="OQL90_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1369361.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8604..9167
                     /gene="pabA"
                     /locus_tag="OQL90_002631"
     CDS             8604..9167
                     /gene="pabA"
                     /locus_tag="OQL90_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1369362.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9253..10473
                     /gene="argD/dapC"
                     /locus_tag="OQL90_002632"
     CDS             9253..10473
                     /gene="argD/dapC"
                     /locus_tag="OQL90_002632"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1369363.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10540..12630)
                     /locus_tag="OQL90_002633"
     CDS             complement(10540..12630)
                     /locus_tag="OQL90_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1369364.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12681..13313)
                     /gene="crp"
                     /locus_tag="OQL90_002634"
     CDS             complement(12681..13313)
                     /gene="crp"
                     /locus_tag="OQL90_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1369365.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3284 a         3491 c         3552 g         3237 t
ORIGIN      
        1 ttatatatta attaatattc attgattata tgaatataag cagactgctc gttaagaacg
       61 atacaggaat atttaattgg cgtcagtgag cgaatgactt aattttgtaa gtcgctgtaa
      121 cggtgaatat aacccattag cagataatat ttattaaggg gttatgtttt atttgctgaa
      181 tatataaaaa gagtcattaa gaaggccaga attctctggc ccgggttaaa ttaatggttg
      241 gagaaccagt tcagtttatc gcgcaggcca acaacccgac caataataat tagcgatgga
      301 ctgttcattt gctgagcaag ttcaccgagt tgcgtgagcg taccgtcaat cacgcgctgc
      361 gtgactgccg ttccgttttc gacaattgcc accggcattt cgccaggcat accgtgttca
      421 atcagctttt gctgaatagt cgcggcctga ttcaacccca tatagaacac cagcgtctgt
      481 ttttctgccg ccaggttttc ccagtccagc tcgccaccgg tttttaagtg tccggtaatt
      541 aagcgtacgc tctgggcata atcgcgatgc gtgagtggaa tacccgaata ggcagagcaa
      601 ccagaagctg cggtaatacc cggaaccacc gagaacggaa tacccgcgtt gcacagtgtt
      661 tccagctctt cgccaccacg gccaaaaata aacggatcgc cacctttcag ccgcaccacg
      721 cgtttgcctt tttgcgcttc ccgcagcagg atctggttaa tctcttcctg cggtacacag
      781 tggtatcccg cgcgtttgcc gacgaaaacg cgatcagcat cgcggcgtac cagattcata
      841 atatcgtcag aaaccagacg gtcgtagacc accacatctg cctgctgaat ttgttgcagt
      901 cctttcagcg tcagcagccc ggcatcgccc ggacctgcac caaccagcac cacttcaccg
      961 cgatggtcga gcggttcgtt gattaactgt tcggtcgttt cagtaatggc tttctgatcg
     1021 ttgtttgcca gcgactgcgc caggcggtcg ttaacgaaca atttctccca gaaacggcga
     1081 cgctcactca tcgtggtgaa ctgttgtttc actcgcccgc gtaattgccc ggcatatttc
     1141 gccacctggc ccagatgcaa cggcagcagt gattcgagtt tttcgcgcaa caggcgcgcc
     1201 agaaccggag aggtgccgcc agaggagacc gctaccatta gcggtgagcg gtcaataatc
     1261 gacggcataa taaagctggc ggctttcggc gcatcgacta cgttacagaa gatgcgacga
     1321 gcttcagcgg cttcgctgac gcgctggtta agcgtgtcat catccgtcgc tgcaatcgcc
     1381 agccagcagg tgtcgagaag gctttcatca aatggccctt cgacgagggt taacatgcct
     1441 gcatctgccc atgcggtgaa ctgtggaata aacgctaatg cattcaccgt taagcgagcg
     1501 cctgcgtcta acagcaacct tgctttgcgt tccgcgacat caccaccgcc gacaatcaga
     1561 cagtcgcgat cgcgtaattg gcaaaatata ggcaaatgat ccacgtaaaa accccttagt
     1621 aattaaccgg cagccgtttc agtttgatta aatttgtccg caaccggacg attcgatttc
     1681 ggcgtagcat accaataacc caatcccatg aatacggcac ctgataaagt attacccagc
     1741 gtcacccaca gcaggttatg accaataccc gccagcgtgt aggcttcgct gtggttgccg
     1801 aaccaggaga gcgcgaacag cgtcatgtta gcgatagagt gctcgtagcc ggacgcgata
     1861 aatgccagca gacaccacca gatagcgata aatttcgccg ccccttcagt gcgcagcgcc
     1921 atccagattg ccaggcaaac cagccagtta cacaatgcac ctttgaagaa gagtaccatt
     1981 gccggtgcag tggttttagc cagcgcgacg gagtgaacga tgctggtatc taccggcagc
     2041 aggctaccgc cgccccagct atagagcatg gcaacgaaga cggaaccgac caggttaccc
     2101 agccaggttt gcggcaggat tgcccacatt tgcccgtggc tgatgctgcc cgcttttacc
     2161 ccaaaggtga ggaacatggt gtgtccggtg aacagttcag aaccggcgat aatcaccagc
     2221 gttaaggcga taccaaaggt cgcgcccatc accagcggac gtacggacgg gtcgagcaaa
     2281 ttaccgagcg tgaaaatcaa gatgatccca agacccacat acgcgcccgc catggcggag
     2341 ctgacccaaa agccgagcgg gttatttgcc gacaggcgtg caatgcgcgc agcgttagcc
     2401 gcacacttat taatagtgtc tgtaaacatt tgattatcct ttaaaaaata aaaacaaaaa
     2461 aataaaacaa aaagtttaaa aaatgtcctg caatataccg aaggtaatta caggaccatg
     2521 caaaaagggg aggcattgcg cctcccgtta aaacattaac cgcgcagctg caccacgccg
     2581 tctttcactc gtgcttcgta atgtttgacg gaaaactgtt cgtcttccat gcacaagcca
     2641 tcacttaagc gaaaacgctg ttttttcagc gggctggcga cccacagctc gccctggtgt
     2701 tccgcaatca gcccgcgtga cagcacgctg gactcgaaga acggatcgat gttgctgatc
     2761 gcaaacacct ggtcgctgtg atacgggcgg aaaatcgcga cctgctcgtc gcctaacagc
     2821 gcgcagacgc cggtttcagg caggatgtca tcgattttgc agatgtcttt ccactggctc
     2881 atgcgttgtc ctccaccaga gttaccggga tacgttcata cggcgttgcc ggacggtgct
     2941 gttcgcgctc tggcaccatc tgcacgttcg ggtcacgctt gtcgctgttg atgaagtgtt
     3001 tgaagcgagt ctgcgcggac ggtgtattga ccgtttcagt ccactcacac actaccgctt
     3061 cacgcaggcg cgccatctct tcttccagat gagcgttcag ccccagtttg tcgtcaatga
     3121 tcaccgcttt caggtaatcg atgccgcctt cgaggttttc taaccacggc gcggtacgcg
     3181 tcagtttgtc ggcggtacgg atgtagaaca tcatgaagcg gtcgagatat ttgatcagcg
     3241 tttcgcgatc gatatcagct gccagcagat ccgcatgacg cggtttcatg ccgccgttgc
     3301 cgcaaacata caggttccag cctttttcag tggcgataat acccacgtct ttaccctgag
     3361 cttctgaaca ttcacgggta cagccggaga caccgaactt cattttgtgc ggcgtacgga
     3421 tgcctttgta gcggttttcc agttccacgc cgaggccgac gctgtcgcca acgccgtagc
     3481 ggcaccaggt gctacccacg caggttttcg ccatacgcag tgctttcgca taggcatgac
     3541 cggtttcgaa gccggcttca atcagctgac gccagatctc tggcagatcg tctttctgtg
     3601 cgccaaacat cgccaggcgc tgggaaccgg tgattttggt gtagagatta aattcacgcg
     3661 cgatacgacc cactgccatc agcccttccg gggtgatttc accgcccgga gaacgcggga
     3721 tcaccgagta ggtcccgtct ttctggatgt tagcgaggaa gttgtcgtta gaatcttgca
     3781 gcggagtatg ttctggcttc agaatgtatt cgttccagca ggaggccagc agcgaaccga
     3841 cggtcggttt acaaacttca caaccgtagc ctttgccgtg tttcgccagc agttcttcga
     3901 aggttttaat gccttcaacg cggatcaaat ggaacagttc ctgacgcgaa taagcaaagt
     3961 gttcgcacag gttgttgtta acttcgatgc cctgtttcgc cagttccgcg ttcagtacct
     4021 gagtgaccag cgggatacag ccaccgcagc cagtacccgc tttggtttca gctttcagcg
     4081 ccgcaactgt gtggcagcct ttgttgatgg cagcaatcag atcaccttta gtgacgtcga
     4141 agcaggagca gatttgcgcg ctgtccggca gtttatcaac accgatagac ggcttaccgc
     4201 tacccgagtg cgccggcaga atcagagaat ccgggttttc cggcagctcg atagcattca
     4261 gcaccagttg cagcaagtta ccgtagtcgc tggtatcgcc caccagtacc gcaccgagca
     4321 gggttttgtt gtcttcgctg acgatcaggc gtttgtagat ctctttgctt tcgtcgaggt
     4381 aaacgtagct acgtgcgcca ggcgtgcgac catgagcatc accaataccg cctacgtcca
     4441 cgcccagcag tttcagcttg gcgctaaggt cagcaccttc aaaggcgttt tcgctaccga
     4501 gaatatggtc aacggcgacc tgcgccattt tgtagccagg tgctaccaga ccaaatatac
     4561 ggttgttcca gcttgcgcat tcaccgatag cgtagatatc cggatcggaa gtttggcagg
     4621 aatcattaat gacgataccc ccacgcggag caacgtccag accacactgg gttgccagct
     4681 tatcgcgcgg acggataccg gtagagaaga cgataaagtc gacttccagt tcgctgccgt
     4741 cggcaaaacg catggtttta cgtgcttcga caccttcctg cacaatctca agggtgtttt
     4801 tgctggtgtg aacgcgcacg cccatacttt cgattttgcg acgcagctgc tcgccaccca
     4861 tctgatcaag ctgttccgcc atcagcatag gggcaaattc gataacgtgg gtttcaatac
     4921 ctaagttttt cagcgcgcca gcggcttcca gacctaacag gccgccacca acaacggcac
     4981 cgcgtttgct gcgacgggcg caggattcaa tggcgttgag gtcttcaatg gtgcgataga
     5041 caaagcagtc ctgagtatca gaacctttga ttggtgggat ccacgggtag gaaccggttg
     5101 ccatgatcag cttgtcataa aaaacggtac gtccggcgct ggagtgaatc accttctcct
     5161 gacggttgat ggtgatagcg cgttcgccga ccagaacttt gatgccgtgt ttctcgtaga
     5221 agccttcgcg caccagcgac agctcttcgg cggtgtggtg agagaagtaa gacgagaggt
     5281 gtacgcggtc ataagcgatg cgcggttctt cacagaaaac ggtaatatca aagttggccg
     5341 catcagattt atcaagaaga tcttcgataa agcgatggcc gaccataccg ttaccgataa
     5401 ttgcgagtct gactttgctc atttttgcct cgatttcttt tctattaccg cctaccttaa
     5461 cgattcagca accccgctta ttgatgtaaa tcaaattcac ctttatatac cccttaaggg
     5521 gtatgttgct gatttgtata aattttctta agtcacggaa ttgtctgaca tttcatgttt
     5581 gtgcaaaaaa tagacaaaat gaacgggttt atagaaatag atgcacgtag cggggttaga
     5641 gaggaaaagg ccggacgacg atagtgccgt ccggcattag caattaatga gaggtcaggg
     5701 tgttatgctg acggtgacgg ctgacgaaac ctaacaggaa gcacatcaca aagacgacag
     5761 cgtacagacc gtttgctgta agcagtgccg cctgcggacc gctatgttca acaatcgggc
     5821 cggtaaccac aaaggtcaac atagtaccga tggttccgca ggtcaggaca aagttaacca
     5881 gttttggcga cggtactttg gtctgctgtg aacccagggt gatgatggtg gtatagatcg
     5941 cgctggagaa gaagcccaga gcgagaattg accacgccat atgtgctggt gttccggtgt
     6001 taaagacgta catcagaatc gcagccagac cagccagtac ggtcagaatg cgttgcaaat
     6061 caaagaagcg aagaataaag ctgaacgccc acatgccgac catgtatgac atccagaagt
     6121 tactcaccag cgtgcccgcg tcgttcaggc tcatgcccag gcctttggca tactcaggca
     6181 cccaggagat aaaacctaac tgaccgagga tgtagcacag cgccgcaaca gagagaaaca
     6241 gtacgccgat cccccacttt tctttctcta ccggagcatc cgtttttggt gcatgtttgc
     6301 ccagtgctgg gaactcacag ccgaaggtca gaataaaaat agcgacatac accagcccga
     6361 tgcaggcata aacccagtac cactcaatgc tgcgcgccag tagaaacgcg gcgatcattg
     6421 ggaaaatcat cccagccata ctgaagaagg agtcagtaaa taacaggcgg gaaccgcgct
     6481 gacgcccttc atacatttgt gttatcagga atgtaccaat cgacatagta atgccgctga
     6541 ccaccccgag aatgaacatc gccgtcgaga acagcgccag gctgtggctg aacatcaaac
     6601 cggcaaccgc cagcaccatc aggagaaagc caaaacgtaa ctgcgttttc aacgggacga
     6661 tttccatcag ccaggcgttg aggaagatag agattaaaat gccggcgttg aggaaggtga
     6721 aggtgttact catactggaa acaggcagat tgaaataatc ggcgatattt cccatcacca
     6781 tcccggtgac aataaccaac gcaccagtca gtgcgtagga gagaaagcta atccatgtga
     6841 gcttgatgcg attgctgtta gtcatgattg gcctgcgttc aaaaataaaa tggcatagcg
     6901 ggatatgccg cgagcgggtg attttaggtg attttgtgat ctgtttaaat gttttattgc
     6961 aatcggttgc taaattgcat tttaagaagt gatcttgatc acggaataaa aagtgatcgt
     7021 caggttacat atatttcaga tacgtaaaat taggtaaagg gatggccttg ttcttgaagg
     7081 ctatttagaa tctcttcact tgcttttttt ctgctctgtt tgttaaggaa atctcatgtt
     7141 caaatcgacc ctggcggcga tggctgctgt tttcgctctt tctgctcttt ctcccgcagc
     7201 aatggcagcg aaaggggccc cgcacgtatt gttgacaacc tcagctggta acatcgaact
     7261 ggagctggat aaacaaaaag cgccagtgtc tgtgcaaaac tttgtcgatt atgtgaacag
     7321 cggtttttat aacaacacta cctttcaccg cgtcattcct ggctttatga ttcagggcgg
     7381 cggtttcacc gagcagatgc agcagaaaaa accaaacccg ccaatcaaaa atgaagccga
     7441 taacggcctg cgcaacacgc gtggcaccat cgcgatggcg cgtaccgctg acaaagatag
     7501 cgccaccagc cagttcttta tcaacgttgc cgataacgcc ttccttgacc atggtcagcg
     7561 tgatttcggt tacgcggtat ttggtaaagt ggtgaaaggc atggacgtgg ccgataagat
     7621 ttcccaggtg ccgactcatg acgttggtcc gtaccagaat gtgccgtcaa aaccggtagt
     7681 tatcctttcc gctaaagtcc tgccgtaatg atttctcgcg cgggcaatct tgcccgcgct
     7741 tctgctctcc cggcgtaacc cggatttgcc gcttatactt gtggcaaatg gacacgttca
     7801 gggaggcatc aagtgaagaa actcaccgat aagcaaaagt cccgtctctg ggagcttcag
     7861 cgtaatcgta atttccaggc cagtcgccgt cttgaaggcg tcgagatgcc tttagtcact
     7921 cttactgccg cagaggcttt agcgcgcctt gtagagctga ggaggcacta tgagcgataa
     7981 attcggcgaa gggcgcgatc cgtatcttta tccaggcctt gatatcatgc gtaaccggct
     8041 gaacatccgc cagcaacaac ggctggaaca ggccgcttac gaaatgacgg cgctgcgtgc
     8101 tgcgaccatt gagcttggtc cgctggtgcg cggtttaccg catttgcgaa ctatccatcg
     8161 ccagctgtat caggatattt tcgactgggc agggcaactg cgtgaagttg atatttatca
     8221 gggcgatacg ccgttctgcc actttgctta tatagaaaaa gagggcaatg ccctgatgca
     8281 ggatctggag gaagaaggtt atctggttgg cctggagaaa acgaagttcg tcgagcggct
     8341 ggcgcattac tattgtgaaa tcaacgtgct gcatcccttc cgggtgggaa gtggtctggc
     8401 acagcggatc ttcttcgagc aactggcgat tcatgccgga tatcaactga gctggcaggg
     8461 tatcgaaaaa gaggcctgga atcaggcaaa tcagagtggg gcaatggggg atctcaccgc
     8521 actgcagatg atatttagca aagtggtaag cgaagccggg gaatctgagt aaaatagcgc
     8581 ggttctttcg taccggagcc gccatgatcc tgcttattga taactacgat tcttttacct
     8641 ggaacctcta ccagtacttt tgtgaactgg gggcggatgt gctggttaag cgcaacgatg
     8701 cgttgacgtt ggcggatatc gacgccctta aaccacaaaa aattgtcatc tcacccggcc
     8761 cctgtacgcc agatgaagcc gggatctccc ttgacgttat tcgccactat gccgggcgct
     8821 tgccgattct tggcgtctgc ctcggtcatc aggcaatggc gcaggcattt ggcggtaaag
     8881 ttgtgcgcgc cgcaaaggtc atgcacggca aaacctcgcc gattacacat aacggtgagg
     8941 gcgtatttcg ggggctggca aatccactta ccgtgacacg ctaccattcg ctggtggtgg
     9001 aacctggctc attaccagcg tgctttgacg tgacggcctg gagcgaaacc cgcgagatta
     9061 tggggattcg ccatcgccag tgggatctgg aaggtgtgca gttccatcca gaaagtattc
     9121 ttagcgaaca aggacatcaa ctgctggcta atttcctgca tctctgattt ccgattgcca
     9181 ttcagtgatt ttttatgcat attttgtgat tataatttca tatttattta tgcgtaacag
     9241 ggtgatcatg agatggcaat tgaacaaaca gcaattacac gcgcgacttt cgatgaagtg
     9301 atcctgccga tttatgctcc ggcagagttt attccggtaa aaggtcaggg cagccgaatc
     9361 tgggatcagc aaggcaagga gtatgtcgat ttcgcgggtg gcattgcagt tacggcgttg
     9421 ggccattgcc atcctgcgct ggtgaacgcg ttaaaaaccc agggcgaaac tctgtggcat
     9481 atcagtaacg ttttcaccaa tgaaccggcg ctgcgtcttg ggcgcaaact gattgaggcg
     9541 acgtttgccg aacgcgtggt gtttatgaac tccggcacgg aagctaacga aaccgccttt
     9601 aaactggcac gccattacgc ctgtgtgcgt catagcccgt tcaaaaccaa aattattgcc
     9661 ttccataacg cttttcatgg tcgctcgctg tttaccgttt cggtgggtgg gcagccaaaa
     9721 tattccgacg gctttgggcc gaaaccggca gacatcatcc acgttccctt taacgatctc
     9781 catgcagtga aagcggtgat ggatgatcac acctgtgcgg tggtggttga gccgatccag
     9841 ggcgagggcg gtgtgacggc atcgacgcca gagtttttgc agggcttgcg cgagctgtgc
     9901 gatcaacatc aggcattatt ggtgtttgat gaagtgcagt gcgggatggg gcggactggc
     9961 gatttgtttg cttacatgta ctacggtgtg acgccggata ttctgacttc tgcgaaagcg
    10021 ttaggcggcg gcttcccgat tagcgccatg ctgaccacgg cggaaattgc ttctgcgttt
    10081 catcctggtt ctcacggttc cacctacggc ggtaatcctc tggcctgtgc agtagcgggc
    10141 gcggcgtttg atatcatcaa tacccctgaa gtgctggaag gcattcaggc gaaacgccag
    10201 cgttttgttg accatttgca gaagatcgat cagcagtacg atgtgtttag cgatattcgc
    10261 ggtatggggc tgttaattgg cgcggagctg aaaccacact acaaaggtcg ggcgcgtgat
    10321 ttcctgtatg cgggtgcaga ggttggcgta atggtgctga atgccggacc ggatgtgatg
    10381 cgtttagcac cgtcgctggt ggtggaagat gcggatatcg atgaagggat gcaacgtttc
    10441 gcccacgcgg tggcgaaggt ggttggggcg taaataagac gcatcaggca aaatatgtcg
    10501 gatgcggcgt gaatgcctta tccgatggtt tagcctgtat tacgccttcg aatcccgcaa
    10561 cttgcgactc agccaaatcc cgtgatgcgg tcgctgacgc catgccatcg acgaaatggt
    10621 gtgcatggtg ttcagatgac caataacccg ctgtaaatgc tgctccagcg tacctgccgc
    10681 gccttcgtgc ggctgcatct ccggcgcatc catgatattg gcatcgccag aactacccgg
    10741 ttcgtcatac tccagtcgct gctgacaacg ctgaatggcg atttcacaag actgtaaata
    10801 ctcttgtgcc agttcaggtg gcaatgcccg gtgttcccgc gccagcgtgg tcatggcatt
    10861 aatatgctca acaataaact ggctgtgcgt tacccacagt ttcatatctg ccagataatg
    10921 gctgttaaac gccggttcct gcatcgcctg attcaatgag ttatacagag tgttatgtgc
    10981 ctgatttacc cgcattcgct gccaggccag tggcgtaggt tgcggatcct cgctaagaat
    11041 caagcgaatc gcttcctgat aggcttctaa agcgtcatgg gcgtttttac gcaataaccc
    11101 gctctgccac tgcggccaca gccagacagt accgccgaaa gcaattaaac aaccaataat
    11161 ggtatcgata agacgcggaa ggatgtattg ctcgccgttc aaccacaata gttgcagggt
    11221 atacactgcg gtaatagtaa aaccgaccgt cgcccagccg tagtttttgc gcaatatcag
    11281 gtagctggcg agggtggtaa tcagcatcaa cgtcagggtg taaccttcgg gaattttaaa
    11341 gtgcagcgcc acgcccgcaa tgattaaccc gaccacggtt cccacggagc gattcacaat
    11401 cctcagacgg gttgcgccat agccattttg tgtcaccagc aataccgtca tcaggatcca
    11461 gtacgacttc ggcagatgca gcgcggtgcc catcaggctg gcaacgctta acatcacact
    11521 gagtcgtccg gcattgcgta gcgccggaga ctttagtgac agataacttt tcagcgcagg
    11581 aagtaatggc attcgccgct gtttatcggc cagtaagtca cgggcataga gcggtttttg
    11641 ggtgcgcagc acgcgggcga tgcggctgaa atgccagtag cagaattgcc caaccggatt
    11701 atccggatgc tggcgggcga ttttttccag tgcgccaatt tgcttttcca tcgtaaaacg
    11761 cgttggcagg cgatggtaaa gaatgtcatc agccagcacg cgcaggcgag cggcgacggt
    11821 ttgcgcattc cagcggataa cttcttccgc atggctacgc tcgaccagct tttgcacctc
    11881 ttccggctga tgcaaactga ccgaaatatg ttcctgtaaa tccagcgcct cctggaaaat
    11941 acgcagcatc cgcttgtagt cagtattatt ttgcgcggaa agcatatgca tttgctgata
    12001 gcactgggta attagatcga ccgctttttg ctggcgcacc agcagcggcg gcagcgcttt
    12061 ttcagggtcg gtgtgctggg taagcaggct gtatttggct tcacaataat ctgccagttc
    12121 acggtacagc agacttagtg actcgcgcag cggttgttcg cgccagatcc agaaccaaaa
    12181 ccagttaaac aatccgtacc agagagtgcc caacgcatag atgagcaacg gttcccagac
    12241 cggcatgtat cccgccagac tgagggtaaa aatggctgcc agcagcgatg cgggaagcaa
    12301 ttttgcgtgc aatggcccca actcagcagt gacgccaagt accagcgtta atccggtcag
    12361 caaaaagggc aggggaacat cttttgccag tagcagctgt gtcagtaagc tacaggtggc
    12421 aaacagcgac gcaccaatga ttaagcgttt gaaaaaacgt ttatgaggcg tatcaaggcc
    12481 cgcaatattg caacaggcag gaacgagaga gaagagcaga ccgaatcgta attcgccaag
    12541 cattaaccca acggccacgg gcaaacatag caccagcgtt tgtcgaagtg catagttgat
    12601 atcggggtga taaatcagtc tgcgccacat cgggggaaac aaaatggcgc gctaccaggt
    12661 aacgcgccac tccgacggga ttaacgagtg ccgtaaacga cgatggtttt accgtgtgcg
    12721 gagatcaggt tctgatcttc aagcatcttc agaatgcgtc ccacggtttc acgagaacag
    12781 ccgacaatct gaccgatttc ctgacgggta attttgattt gcataccgtc cgggtgagtc
    12841 atagcgtctg gttgttttgc cagattcagc agagtctgtg caatgcggcc cgtcacgtcg
    12901 aggaacgcca ggttgcccac tttctctgaa gtgacttgca gacgacgcgc catctgtgca
    12961 gacagacgca tcagaatgtc cgggtttacc tgaatcaatt ggcgaaattt tttgtacgaa
    13021 atttcagcca cttcacaggc ggttttcgca cgtacccatg cgctacgttc ctggccctct
    13081 tcaaacaggc ccagttcgcc aataaaatca ccctgattca gataggagag gatcatttct
    13141 ttaccctctt cgtctttgat cagcactgcc acagagcctt taacgatgta gtacagcgtt
    13201 tccgcttttt caccctggtg aataagcgtg ctcttggatg ggtacttatg aatgtggcaa
    13261 tgagacaaga accattcgag agtcgggtct gtttgcggtt tgccaagcac catgcgcggt
    13321 tatcctctgt tataagcttt ctccagagcc agataacgcc gctgtctctg gattgccgaa
    13381 atatgcttcc cgctacctgg gaaggggcta tcaactgtac tgcacggtaa tgtgacgtcc
    13441 tttgcataca tgcagtacat caatgtatta ctgtagcatc ctgactgttt tagcatagct
    13501 ttcgctttgt gtctcctggt gtctcgcttc agcatgaccc aggtcgcctt ccgttgcgcg
    13561 attt
//
LOCUS       DAJVOY010000101        13527 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVOY010000101 DAJVOY010000000
VERSION     DAJVOY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.101"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..529
                     /locus_tag="OQL90_002635"
     CDS             <1..529
                     /locus_tag="OQL90_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCS1369366.1"
                     /translation="NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYE
                     SNVKSGGVGARYFGIGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAG
                     VFRFIDYQRLLEGEVGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQN
                     DILVKYRHMSVPPES"
     gene            complement(593..1084)
                     /locus_tag="OQL90_002636"
     CDS             complement(593..1084)
                     /locus_tag="OQL90_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1369367.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1186..1740)
                     /gene="ycdY"
                     /locus_tag="OQL90_002637"
     CDS             complement(1186..1740)
                     /gene="ycdY"
                     /locus_tag="OQL90_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1369368.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1764..2501)
                     /gene="ycdX"
                     /locus_tag="OQL90_002638"
     CDS             complement(1764..2501)
                     /gene="ycdX"
                     /locus_tag="OQL90_002638"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1369369.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2556..3494)
                     /gene="ghrA"
                     /locus_tag="OQL90_002639"
     CDS             complement(2556..3494)
                     /gene="ghrA"
                     /locus_tag="OQL90_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCS1369370.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3728..3815
                     /locus_tag="OQL90_002640"
     tRNA            3728..3815
                     /locus_tag="OQL90_002640"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3762..3764,aa:Ser,seq:gga)
     gene            complement(3965..4807)
                     /locus_tag="OQL90_002641"
     CDS             complement(3965..4807)
                     /locus_tag="OQL90_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCS1369371.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKTIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(4892..5089)
                     /locus_tag="OQL90_002642"
     CDS             complement(4892..5089)
                     /locus_tag="OQL90_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369372.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(5101..5592)
                     /locus_tag="OQL90_002643"
     CDS             complement(5101..5592)
                     /locus_tag="OQL90_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369373.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(5589..5963)
                     /locus_tag="OQL90_002644"
     CDS             complement(5589..5963)
                     /locus_tag="OQL90_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCS1369374.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(6010..6384)
                     /locus_tag="OQL90_002645"
     CDS             complement(6010..6384)
                     /locus_tag="OQL90_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCS1369375.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(6547..6768)
                     /locus_tag="OQL90_002646"
     CDS             complement(6547..6768)
                     /locus_tag="OQL90_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCS1369376.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6831..7307)
                     /locus_tag="OQL90_002647"
     CDS             complement(6831..7307)
                     /locus_tag="OQL90_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCS1369377.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7323..7796)
                     /locus_tag="OQL90_002648"
     CDS             complement(7323..7796)
                     /locus_tag="OQL90_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCS1369378.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(8138..8956)
                     /locus_tag="OQL90_002649"
     CDS             complement(8138..8956)
                     /locus_tag="OQL90_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCS1369379.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(9111..9269)
                     /locus_tag="OQL90_002650"
     CDS             complement(9111..9269)
                     /locus_tag="OQL90_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCS1369380.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(9340..12186)
                     /gene="ag43"
                     /locus_tag="OQL90_002651"
     CDS             complement(9340..12186)
                     /gene="ag43"
                     /locus_tag="OQL90_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCS1369381.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            complement(12558..13430)
                     /locus_tag="OQL90_002652"
     CDS             complement(12558..13430)
                     /locus_tag="OQL90_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCS1369382.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT         3159 a         3640 c         3513 g         3215 t
ORIGIN      
        1 aaacctgctt aacgagcgca agattattcg tgcggcacaa gaaaacggca cggttgccat
       61 taataaccga atcccgctgc aatctttaac ggcggcaaat attatggttg aaggttcgat
      121 tatcggttat gaaagcaacg ttaaatcggg cggtgttggg gcaagatatt ttgggatcgg
      181 tgcagacacg caataccagc tcgatcagat tgccgtgaac ctgcgtgtcg tcaatgtgag
      241 taccggtgag atcctctcct cggtgaacac cagtaagacg atactttcct atgaagttca
      301 ggccggggtt ttccgcttta ttgactacca gcgcttgctg gaaggggaag tgggttacac
      361 ctcaaacgaa cccgttatgc tgtgtctgat gtcggctatc gaaacagggg tcattttcct
      421 gattaatgat ggtatcgacc gtggtctgtg ggatttgcaa aataaagcag aacggcagaa
      481 tgacattctg gtgaaatacc gccatatgtc ggttccaccg gaatcctgac agataaaaaa
      541 gccggaatta tcatccggca ttttactgtt taagcgaaag cgtgagtgcc cctcacgctt
      601 tatttttgat ttcctgctca cggtgagccg tctttgcact acgcgccgcc agccacaggc
      661 cgctgttttt cattgcgtaa ccaaatacca gcccaagcgc cagcgaaggt aacaccagtt
      721 tccagtcacc ctgcccggca aacgtcgcgc atgcgcctat aaacgtcccc ggaacaaatg
      781 aaagcagcag ctgtttggcc tgaatacaca tcagaaacgc cacaataccg gttatgacat
      841 aaccgagaat ttccagatgt ggtgccagtg cactaccgta aataatgacc atcgcccaca
      901 ccacgccact aagcagggtt gcagcggaga tcgccagccc tttcagccca ccttgcgggc
      961 aggcaaagta ggccgtacag cccaggaagc ccgcccagct tagtaagccc agggaaacag
     1021 ccacccatcc ccagataccg gagagaatgc ctgttgtgat tgcaatagag agaagtatgt
     1081 tcatgccgca catgatagca gaaaacgcgg ccatgaatga gatcgagcac acattttatg
     1141 caacaacaaa ttatctgttg cagttatgag tgacgatgat cacatttact cttcagaatc
     1201 ttcttccagc tcgtcccaca ttgcactaat ggcatcacgg gttagcggtg ccatggtgcg
     1261 ccagaaaggc gtggttgcat gggcctccac tttgccgagg aacgcaccac accagggtaa
     1321 cagatactca ctgaacagtg tttccagtgc ttcgctctca tcttccgttg actgatcttc
     1381 catccaggaa gccgcaagca gcaatgtgcc gatgtgatcg gctggcgtat ccgccagtgg
     1441 catccctcgt tcggaaagaa aagcgcgcac ttccgcttcc gttgcgtcct caacccatgc
     1501 gctacgatat ggcggcacgg cacattcatc gccgataaac aacgcattgt aatcggcaga
     1561 gacttgcgcc atatcacagc ttttctgtaa acgcgtcagt aactcatcct gctccagtgg
     1621 ccagttcgca gccagtttcc cctcacgaat cagggtaaac agcggcacca gtaaaggatc
     1681 ttgcggttgg cggtaataga gcgaacccag cacacgacag aggatagaaa actcgttcat
     1741 taatatattt ccattactag taattaaagg tctgcaaatt ccgcaatcgg tgccataccg
     1801 cgagattcaa ggaagttcag taagcggcgc ggagaaacat tcaaaatgcg ctctggcgga
     1861 aaatctaccg cgtcgaggat tttaagacac tcttcaaatt cccccatggt aaacgcggtg
     1921 tgagaatccg agcctaatgc cacccaacct ccagcatcac gtaccgctgc ggctaccgca
     1981 cgacagttgt cttcactgcc cttacgtgag tgtaaaaatg aagaattatt gatttccagc
     2041 gccacctgat gtttcgcggc tgcttcagca acggctttca catctatttc atatttggga
     2101 tttccgggat ggcttattat atgcacattg ccgctggcga tagtggcgat catcgcttgt
     2161 gtattggtcg ctttgtcatg tggtgcaaaa accggctcat gaaaaccggc aataatcaga
     2221 tccagcgagt caaacatttt accgctgcaa tcaatttcac catcaacatt tttaatgtta
     2281 gcttcgatgc cgcgcaggat ccctacccca tcaaccactc gcggccagat acgcatgtta
     2341 atgaagtgcc agtgatgcgg agcatcttcc atatccgggc catgatcggt gatcgcaaaa
     2401 agtttaatgc ccttttgttt ggcctgggca atgtaatcac tcaatgtgct atatgcatgt
     2461 gtgctggcaa cggtatgcat atgaaggtcg acgggataca taactctctc ctgtattcaa
     2521 ttcccgccaa ggatagcagg aatcctgatg ctttattagt agccgcgtgc gcggtcgact
     2581 tgcccgcaga ccctctcccc tttttcgagc tgggcaatgg tgcgagaaat gtactccaca
     2641 gcttcagcgg gacgggtaat cgcggcgaca tgtggtgtta tcgtcacgcg tggatgttgc
     2701 cagagcggac tttcaggcgg taagggttca cgattaaaaa catccaacat tgcgccttta
     2761 actttgccgc tatccagcgc cgcgagcagg tcatcttcca caacatgaac accacgcgcc
     2821 aggttgagga gatacgcgcc atccggtaat ttttcgagta attgttgatt aataatgccg
     2881 acggtttcag gggtattcgg taacaaatta atcaataccc gacattggct cagaaatgca
     2941 gacagttctt cccgtccggc aaagctttgc acgccaggcc acgatttacg ggttcgactc
     3001 cagcaacgca gcggaaagcg ccaggtttgc agactctgag caactttact gcccagtacg
     3061 cctgcgccca aaatgccgat ggtaaaatct tcccaatgat attcaggcag cggttgccaa
     3121 tgcgaactat tttgctggat gcgataatcg tcaaaacgtc gaaaccaatg cagcacctga
     3181 ctgacagcat attcctgcat ttgctcgccc ataccagtat cttccaggcg aaaaagtgga
     3241 acagaagggt tcagcatttc agggtgtgcc tgtagcttgc tcaaaataga atcaacaccg
     3301 gccccgagtg cgaacaccgc tttaagatcg cgccctgcca gcatttcaac aggaggatgc
     3361 cagactaaag cataatcagc agagtcatta tctccgcttt tccatgctct gactcttgcc
     3421 tgaggaatag ctttgcgcag tgcctcaatc caccattggg tatcgaacgt tgggtgataa
     3481 aagatgatat ccatactgac tcccgaaaag cgttgtgcga aatttattcg cacttatcgt
     3541 tatgatctac aaaggccacc agcataacaa atccgtggtc ggtggcaaaa aaagcagatt
     3601 tcgcttatta aaaccacaca ttgattgaag tttgaataaa cgcgcgattt tttcaaaaag
     3661 tttgttgacc tcaggtcatg atttccctaa attagcgccc gttccagcaa gacaggaaca
     3721 acaatttggt gaggtgtccg agcggttgaa ggagcacgcc tggaaagtgt gtatacggca
     3781 acgtatcggg ggttcgaatc cccccctcac cgccagatta taagcgcatg ttattgttta
     3841 acatgcgctt ttttattttt ccttccagac agtatgccat cgggtatgcc atctgaaacc
     3901 ggtttatatg ccccgcgatc atatcttatc ggacaatttc cgatctttga gcatactggc
     3961 aaagctatac tgccagcata tcaggatagt gttttgcgat aatatcattc agtctgtcaa
     4021 ccagttctgg ttttctgaac gtgatatgcg cggagccctt cttaaagtat ctgatgctga
     4081 acatctcatc ttcatagctc tctttaccct gaacagactg aatgtgatca tccagacgaa
     4141 gggtgatgtt tccccggttg tcggggattg ttttaccgct gaacaggtgc aacattcttt
     4201 ccagatcggc gagtcggtca gcctgttgtc cggtgataag atgaaatccc caccggtccc
     4261 atctcaccag attgttgacg ataattttac tgccaaattt acaggggcaa ttggttttgt
     4321 aattccagtt cagccctctg aagacgttga tcacccctcg ctcaaacacc tcatccttat
     4381 tctggtgcag ttgttcaaat gtgctcagaa tgttcgcttc gctgatttcc ggaaaattat
     4441 cgtactccag tgaccggtac catgtatcac gggcctgcgt atccattaat gacagcatcc
     4501 cggaacgttg catcaggtca cgccagattt cgcgatcaag attccgggta atggctttca
     4561 ttgctgtttc gggtttctcc atcagccagc agccgcagcg aaaatcctgt ttcatggccc
     4621 agtccggggc agttttacct ccgatgcttt ccgtcagcac cgagatttca gccagttgtc
     4681 ggataagtgt gtcgatttgc aacagtgtac tctttcgtac agccagaatg tggcgaatgc
     4741 tggttgagca gataacgtcc gtatgcccgt tgaccacttc cggacgggag attgtgatat
     4801 ctgacataat cgatattcct ttttgcagat aaatgaaaac gccagccctg tgcaggctgg
     4861 cgtcagagga atgaagtctg gtgagtgagc ttcaccggtt ctggtgcaga agctgcagat
     4921 gacgcagggt acggacatcc tcccgggcct gttcgataca gggcaacagt gctgctgaat
     4981 ccgttttatc ctcatcgttg tcaaagataa ttcccgatcc gcagtcgata ttgtcctgca
     5041 gccacgcaat cagaatatcc agcgctgttt ccgtggttaa tgatttcatg ttgttcctgg
     5101 ttaccagtcg aaagtgggta aacccggcag gcattcagcg ctggcatcca gatgaatgag
     5161 actgacgcca taacgccgga tgagtgtgac gaccagacga cggaacgttt ttgacagccc
     5221 caggcgttta agccgcagaa ccgggtatga ccacgcatcg gtacgtaaca gatatccgct
     5281 acctgtaaaa tgaatccatt ctgattcacc gaagtcactg gtctggtgtg acagcgagta
     5341 caaccaggcg ttgtccgttt ccgtgatatg tgcggtactg cagcgtatgc cggtaagggt
     5401 agcaaatggt ggcgtgcatt ctgctgaggt ctggtcagat tcatcaacaa cacgaaggga
     5461 gtagccgtta tcgcagacca tgttaatcag ttcagcgagc tcaataccat cgacgtcaac
     5521 gacaatgcgc cccatgttca gtgcctgtac gttaacgctg tcggcttcca gtgtcagggc
     5581 cagtttcatc atttcgcctc cggatactta cccagggtaa tgttatttac cgttctgtaa
     5641 ttgtcgcggg tcatcaggcc ggtcgcccgg cgagcccgga ggatatcaat actgtttatt
     5701 aactgagagc ggggacaggt gctgaatccc ggctggtcgg tacgcaccag cgcgtatttt
     5761 tccacgagaa aattaactgc atcacacagt gaaatgcctg cctcaatatg ctgctcaatc
     5821 acacgttcat cgacaaacgg tgtgtcattc agtgtgaggc cataatgctg gtccagcagt
     5881 cggatgagca gtgtctgcca gatggtgacg ggagacgggc agcgagatac ctcccgtaca
     5941 tgtgtgtcgg ataatgtttt catcttaagt tctctgtaac tggtagtgct aaaggtgaga
     6001 gcttcggggt tatgaggtgg ttgcgggtgc tggtgtcgga taaacagcca gataaacgta
     6061 accacaggag ccgagggtgt cggcttcgca ggtcagccct tttgcataca gcgtgacggt
     6121 atgctgatat cggggattca gttcaccgct ggtgagcatg agttccagtt gtttcatcag
     6181 cagcggaaag gcctggtcca gatggtatgc atctgcgtcg ctgaagcggc ctctgatacc
     6241 ggcgcggtcg gcaaggtaat acaggcggtt accctcttgc accagacgag cgccaaaaca
     6301 gggcctcact gtgcagggaa gcccccacca ggggcggtcg tgattgtcat cgggatgagt
     6361 tatcccggag agtttgtctg acacgataaa atcctctctg aaaagtggtt aagaatggtt
     6421 taacgatgac aggtcagccg gcgcgctgac cggtttcggt gaagatgtat gcattgatga
     6481 gaatggcctc atccacagcg gcgtctgagg tcatctggtc gtactcgttt cccgggtgac
     6541 ggaacatcag ttcagggtga cactcatcag ccggacatac gggccaaaac tgtccttacg
     6601 gcgttctgca aacacggcaa gaacgccggg aatatcctgc acttcacgac cagtatacgc
     6661 ttcagcactg ccgtgccagc gatacttacc ggtacagaac ggaaaaagac gggatgcagg
     6721 atgctgttgg tgaatacgca tggcttcacc acgggtgatg attctcatga tgtgatacct
     6781 ctgaagacag aagataaaag tgaaaacagg tgtgatgtgg ttgtgacggg ttaaagcaga
     6841 ccatgttcgg caaaggagaa aacctggttg ccaccgacta tcagatggtc cggcacccgg
     6901 atatccacca ggcccagtgc ctgtaccaga cgttcggtga taagccggtc tgccttactg
     6961 ggtgtgactt caccggacgg gtgattgtgc gccagcacca cggcagcggc attgtggtac
     7021 agggcgcgtt taatcacttc ccggggatga acttccgtac ggttgatggt gccggtgaag
     7081 agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg gaactcttcc
     7141 cgctccagtc ccgccatgtt cagaatcagc cattcccgtg ccgcacgggt ggaggtgaag
     7201 gccacgccgg gttcatgaag atggcggtcc agggtttgca gggcccgcag aatgagactg
     7261 cgctcgccgg gcgtcatgtt tccgggcaga aaggaaagct gctgcattgt ccctgcctcc
     7321 attcagtcga tgatgcgcat aatggcgctg cattccggat gctgcagggc gtaatcccgc
     7381 agccggtaat aatggaccgt catggcataa cactccgtac gacaggcatg atgactgtac
     7441 gtcatcagac aggcagcaat gccggcggct tccgggctca tttcagcgcg gttaccgttc
     7501 atggcattga acagtaccca tgtttcgtca tcatcgtcat ccggttcggg tgccataaat
     7561 gccccgccgt tgttcagggt gtacagattc cagataccac cgcagtagtc ttcacacaga
     7621 cggtccatcc agccgaagac acggggttcc agggtgaccc actgtggaat gaggccaaaa
     7681 tgctgcggcc agaagctgat gcgctgttca tcagggacgg gagtggcaac cagttgttgc
     7741 aggccagtct ccggtgcaca ggccgaagcg gtggtgttct gagacaatgt tttcatgtat
     7801 gaaatccttg taatgtgaat aaatgagagg gagtaaaggt cgggagctgg caacatcacg
     7861 ttcatcactg accgtgatga cccggtaata tcagggatga atgatattcg gctgaaattc
     7921 cggacagccg tacgcgtaac cgttccggtc acagacggat ggccagatgc cattgcctgt
     7981 atactcagtc tgcacagact gatttctccg ggacggtaaa gcagacgggt acaccaggcg
     8041 cacaggtcat catcagaaat ggaacgacac cccggcagcg cctccggggc gtcgggtgat
     8101 tctggtgcct gaatcacgcc ttcagggaca tgctgcatca gcgcaggctc tccagcagcg
     8161 tttctgccat tacccacaat gcgcggttga gcttaatatc ggtgtcgatg ttgtgaatgg
     8221 cacgggtgtg gatacgtttt ccttttgcac tgcggccgga aatcccgcct ttcagcatat
     8281 tctcctggat ggtctgatat gcgctccaca ggtctttacc gtaatcctcc ctgcgtcgtg
     8341 gtgtcagaat gtcagcggtg gtgactggct gatgttcgtc accataacgg taagtcagtg
     8401 ccgcctgtgc cagcgcctgg cgtgccggtg gcggcaggac cagcgactgc atggcatcac
     8461 gcttttcctc aatccggtca aaaaccccca ccacttcgta agccccttcg ataactttct
     8521 ccaccacatt tccccggtgt ggaacacgca cttcccccag agactgaccg cagacgcagc
     8581 cattctggca gacgaatctg aagtaacccg gcagcatctg gtagctggag gtaccgtcat
     8641 gagagttgag cagaataatt tcagggacat gttctccgtt tatctctccg gcccgccgca
     8701 gacgcagcat gtgttttgtg tatccccggc ggcccgggtc gcgcacgcgg gtctggcagg
     8761 cgaagaatgg ctgaaaacct tcctgctgca ggctttccag tacggtgatg gtgggaatgt
     8821 acgcatatcg tttactgcgg gaagtatgcc ggtcttcacc gaaaatactg ggaacatagt
     8881 gcatcagttc ttcgtgtgtc agcggacggt cacggcgtat ctggttcgca taaccaaaac
     8941 gactggctag tcgcataatt tgctccttat cggtggttac gatttactgg tgtaataaat
     9001 gaaaaagctg cgtctcccgg agaagacgcg gcctgacaga tgaaatgaat gacgtttatt
     9061 gtctgagaag cccttaactg gcgagctgag tattaagctg tgttccggca tcaccagcgc
     9121 aactgacctt cagcattacg gataaccagc cgggaatatg ttccctggtc atcttcagta
     9181 aacacattgc ggtaagctgt tttgacggca acagcctgtt cgcgtgagaa agggccttcg
     9241 ggcaataaac gtgatgtaca acccggcata tctgttgctc cctgaaagta aaaagccccg
     9301 gtcatgatga ccggggcctg aaggagagtg acctgattat caaaaagtca cattcagcgt
     9361 ggcctgaccg ttataacctt cagcgctgct gccgctgacg ctgtgggcat aaccggcctg
     9421 aacgcccagg gtgatatttt cccggacacg ggcttccagt ccggcctgca ggtccagtga
     9481 cgtgccattc cgggacggtg agaacgtcat gttactgccg gcggcggctg tccccatgct
     9541 catgtcaccc cgggagctga aagtgcggat aacagaaggc tgtacccacc agttcaccgg
     9601 cagttcactc acacggtgtt ttgtactgtc gcgcagagtg tcacgggatg aggtgccttc
     9661 accaaagctc atatcgttgt ggctgcccag acggaaaccg gcacgcacat gttgtgcact
     9721 gccatgcccg aacttcacat aaccggcgtt atcctggccg tcatccaggg taagtccctg
     9781 ccaggtatag tgcagttgtg gctccagcat caggttgtca gtgatactga agggcagacc
     9841 ggtttccagt gagcccagcc agccccagcc ccgggcacgg aagtcgttat tgtccgatga
     9901 cgctttcatg ctgtggcggg ttccctgtgc cacaatgtca gcccacaggc cggaggacgt
     9961 gtgtgtcaga ttcaggtatc cgcccaggct gccggcatca tcccggaccg tgccggcgcg
    10021 ggaaccgtca tcatccttaa catcaacgga agaatggcct gcggcaccat acacccctgt
    10081 cgtcagagac ataccggcaa cctctgttct cagcaggtca ccctccagac ggacgaagcc
    10141 atagctgccg ctgctttccg gcgtggctcc acgggcaata ccgccgttgt tatcgtgacc
    10201 gagatgaccg ccctgaatgc tgagacggac gctgttattt tcaccgttta caccggtctg
    10261 atggctgcgg gagcctgcca gaatccggtc atagtccatt gcctgtgtca gcatggatgt
    10321 atacaggggg acttcagcac gataagcatt ttcactgcgc aggtaccagt cttcatcgct
    10381 gtcacggttc agggtgtagt taaaggcgcc ggcctgaagc gggcgactca gggcaaacgc
    10441 accttcttct gtggtggcgc cattctgtgc atccacaacc cggataccct gtccggaggt
    10501 tgccaccccg aggttactgt ttccgacatt tgtaaacgca agccaggttt tgccggttgc
    10561 ctgaccacca ttaatcacca gctggtcaga ggtattgctg ccatcaaggc gaacacgcat
    10621 attgatggtg ccaccctgac cggtgaggtt actggtggtc agtttatgga acgttaccgg
    10681 ggagtcgcct tttgcaacag cacggctcag cgcggcatcc ggtgtcgtct gattatcaaa
    10741 gcgaatctcc cctgcgttaa caatatcgcc ggtactgacc acatcgccat tcagtaccat
    10801 gctggcacct ttactgagct gtgttctgcc actcagactt cccccggcag ccagcgtcag
    10861 tgttccacga tgtccgacag tggtgttgct ggccagtcct ccggcattaa ccgtaaagct
    10921 gccgccattt tccagcagca gtccgctggc ctgaccggat gtgccatcaa tactgaactt
    10981 accgctggca ttggtcccct caacagtggc accactgtct gcaatcaggg caccaccgga
    11041 tgtcatggtg acacctgttg ccttaccacc ggcagacact gccagggttc cgccgtcatc
    11101 cacccgtgtt ttctgcgctg aatggccttc cagtacatcc aggcgaccgc cggactccag
    11161 aacaactccg tcagccttac cgttttccac cgtgaaattg cccagacggt tgctgccaag
    11221 aaccgttgcc gcagtactgg taaccagtgc tccaccctgt ttcagggtga catgtgtggc
    11281 ggtaccaccg gcgttcacct gcagtttgcc tttctggtta acggtggtga aatccgccag
    11341 accaccttcc ttgataatct gccagccgtc actgtttaca acagtgccag acgccgtacc
    11401 gccgttgtgc acatactggt aaccgccatt cagtgtggta tccagcgcat gcccgtgtac
    11461 cgtctggtcg ccgccggcat aaaccacagt ggtatttgct gttccttcag cagccacaat
    11521 ctgacgacca ttttcattga tagtggtacg tacggcattt ccgcgaacaa actgcccggt
    11581 atcaccattt tctgcatctg gtcccccttc cgcgccggta ttcacaaccg tgtcggttgc
    11641 cattgcgccg gatttgacgg tctgccagcc cttctcatta atgacggtac ccgttgcaat
    11701 cccgccttca tgtacccact gctcaccgcc gttcagagtg gtattcactg cctgcccctg
    11761 aaggctctgt ccgcctccgg cactgataac cgtgtctgaa acgcttcctc cggcattcac
    11821 tctctgaaga ccaccaccgg tgacagtagt gttgttggcg ataccgccat tttgtatcca
    11881 ttgcccgccg gtattggcct cgttatccgg cccatactcc agaccgctac tgatggtcat
    11941 tccgttggcc gtaccgagaa caatctggtt gtcatgattt gtcagtgttc cgccgttcac
    12001 ggtttctccc gcctgtacaa tcgtgtcagc agccagtgcc gggactgatg tgacagcagc
    12061 aagagacagc acaaccgcca caccggcgcg ttttccccgt gagcgggcca gttcggaagc
    12121 caccaccagg gtgcccgtaa tgtgattcca taccagcctg tagctggtgt tcagatgtcg
    12181 tttcatcagc ttttccttac agagggtgaa taaaaaagcc ggtacccaca aaagtggata
    12241 ccggcaggta caaaacagca tggtcagata aagtggtttt tcccggacat aaatgcttcc
    12301 cctctctcat ccgccagaga agtatcagtc cgttgtgtgc aattccgttc tgcatgatga
    12361 agagattcag ctgccttttc gcagaccatt aaccactata aacgatcgat agcaacgatt
    12421 gatacctgtt ttatcgatcg ttttattcta ttaacgacaa ggcggtcacg caagagaaac
    12481 aggaggagta tgaggggttt gtgtcatgtt ttcagttggt taacgtaatg ttatcaggca
    12541 tttccctggc agactgttca gaagaaaatc cagttccata cagcgcgggc aacagagacc
    12601 accgtgtcac ggacggcacg cagaaccgta cgggcaacag aggcaacaca gacgctctcc
    12661 gccgtgtcaa atatccggtc caccgcaccg gtaaactgtt cacgggcctg accccggaca
    12721 gattccgtgc gcagctcgtc ctgcaggcgg gtcatcaggg gactggcggc atggtcggga
    12781 agtgctgtca ttagcgcact gaccagcgta tccagttccc agccggtgcg ggccgatacg
    12841 gcaacaaccg gatgtacggg ccggaacaga cggaataccg cctccgtttt ttcgcgaata
    12901 ttctgtgcct gtgcgggaga aggctgaata ccggccatat cccattcatg gcagggctcc
    12961 gttttgtcgg cctgcgtcac cacaaacagc acctgctgat gtccccggtg caggatgtgt
    13021 cgccagaaat actcatccac agacagggca cggtcatcgg ctttaatcag ccacagtacc
    13081 aggtcaagtt caggcagaat gtcacggtac agggcttcat actctgcatc tctgtcccgg
    13141 ctctcgccca ccccgggcag gtcagtgata accatgctgt gaccatggcc actcagacgg
    13201 aaacgctgca cttcccgggt gccggcgtga acatcactga ccggggtgac ctccccctga
    13261 aacagtgcat tacagagtga ggatttaccg gccccgcttt tacccataat gccaatcacg
    13321 ggttcgtgac tggtgagttt gcgcagatgt tccaggatgt gacgggaaag tgagtaaggc
    13381 agggaggaga gcggtttttc aattgcctca atggcatcag acggattcat acatgtattc
    13441 ccggtgaaaa aagacaaaac ccccgcacac cggaaaggtg gcgggggact cacggagata
    13501 atatatgtct gaaattatct ggaatgt
//
LOCUS       DAJVOY010000102        13481 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVOY010000102 DAJVOY010000000
VERSION     DAJVOY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.102"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(104..3325)
                     /gene="carB"
                     /locus_tag="OQL90_002653"
     CDS             complement(104..3325)
                     /gene="carB"
                     /locus_tag="OQL90_002653"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1369383.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3343..4491)
                     /gene="carA"
                     /locus_tag="OQL90_002654"
     CDS             complement(3343..4491)
                     /gene="carA"
                     /locus_tag="OQL90_002654"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1369384.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4946..5767)
                     /gene="dapB"
                     /locus_tag="OQL90_002655"
     CDS             complement(4946..5767)
                     /gene="dapB"
                     /locus_tag="OQL90_002655"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1369385.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5934..6848)
                     /gene="rihC"
                     /locus_tag="OQL90_002656"
     CDS             complement(5934..6848)
                     /gene="rihC"
                     /locus_tag="OQL90_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1369386.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6914..7864)
                     /gene="ispH"
                     /locus_tag="OQL90_002657"
                     /gene_synonym="lytB"
     CDS             complement(6914..7864)
                     /gene="ispH"
                     /locus_tag="OQL90_002657"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1369387.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7866..8315)
                     /gene="fkpB"
                     /locus_tag="OQL90_002658"
     CDS             complement(7866..8315)
                     /gene="fkpB"
                     /locus_tag="OQL90_002658"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1369388.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8384..8878)
                     /gene="lspA"
                     /locus_tag="OQL90_002659"
     CDS             complement(8384..8878)
                     /gene="lspA"
                     /locus_tag="OQL90_002659"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1369389.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8878..11694)
                     /gene="ileS"
                     /locus_tag="OQL90_002660"
     CDS             complement(8878..11694)
                     /gene="ileS"
                     /locus_tag="OQL90_002660"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1369390.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11737..12678)
                     /gene="ribF"
                     /locus_tag="OQL90_002661"
     CDS             complement(11737..12678)
                     /gene="ribF"
                     /locus_tag="OQL90_002661"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1369391.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12686..12904)
                     /locus_tag="OQL90_002662"
     CDS             complement(12686..12904)
                     /locus_tag="OQL90_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1369392.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13007..13270
                     /gene="rpsT"
                     /locus_tag="OQL90_002663"
     CDS             13007..13270
                     /gene="rpsT"
                     /locus_tag="OQL90_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1369393.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIKAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3149 a         3807 c         3397 g         3128 t
ORIGIN      
        1 ccaaaagatt tacttaatgg gcaaaaaaaa cggtcctcat cagagaaccg tattacttat
       61 tcgatcaaat tagcggatga aaaatatccg ccatgacacg ctattatttg atctgcgcgt
      121 gcatttcctg caccgaaatt actttttcgg tcgcgtcggc attcagcgcc atcgcggtag
      181 cgaaaccacc gttcagggtg gtgtcataat gcactttata ttgcagcgca ctgcgacgga
      241 tcacgcggga gtcttcaatc gcgcgacggc ctgaagtggt gttgatgatg taggtatatt
      301 cgccattctt gatgcggtcc tgaatgtgcg gacggccttc atgcaccttg ttcaccagac
      361 gcggattaat gcccgcttcg cccagcacaa tcgccgtgcc gtgggtcgca tccagctcga
      421 agccctgttt cagcagtttt gccgccaggt ccaccacgcg ttctttatcg ccttcgcgca
      481 cggaaagcaa cgcacgaccg tgtttcttca tggtggagtt gctgcccagc tgcgctttgg
      541 caaacgcttc agcgaaggtg cggcccacgc ccatcacttc cccggtggag cgcatttctg
      601 gccctaacag cgggtcaaca cccgggaatt tattgaacgg cagcaccact tctttcaccg
      661 agtagtacgg cgggataact tctttggtta cgccctgctc agccagcgat ttgccagcca
      721 tcacacgcgc cgccactttt gccagcggta cgccggtggc tttggagacg aacggaacgg
      781 tacgcgccgc acgcgggtta acttcaatca ggtagacttc gttgtttttc accgcaaact
      841 gcacgttcat cagaccgcgc acctgcaatt cgaaggccag tttctgcacc tgctggcgca
      901 tcacatcctg aatttcctga cttaaggtgt aggctggcag agaacatgcg gagtcaccgg
      961 agtgcacgcc cgcctgctcg atgtgctcca tgatgccgcc aatcagcacc atttcgccgt
     1021 cgcagatggc atccacgtca acttctaccg cgtcatcaag gaagtgatcc agcaacactg
     1081 gcgcatcgtt agacacgctg accgccgtct ggaagtagcg acgcaggtca gcttcgtcat
     1141 agacgatttc catcgcccga ccgccgagaa cgtaagacgg acgtaccacc agcgggtagc
     1201 caatctcttt cgccttctct accgccattt caatagcggt aacggtggcg ttcgccggtt
     1261 gtttcagttt cagacgctca accgcatgct ggaagcgttc acggtcttct gcacggtcga
     1321 tagcatccgg gctggtgccg ataaccggta cgccagcagc ttccagcgcg cgcgccagtt
     1381 tcagcggggt ctgaccgccg tactgaacga taacgccttt cggcttctca atacgcacga
     1441 tttccagcac atcttccaga gttaccggct cgaagtagag gcgatcggaa gtgtcgtagt
     1501 cggtggagac ggtttccggg ttacagttaa ccataatggt ttcgtaaccg tcttcgcgca
     1561 gcgccagcga ggcgtgtaca cagcagtagt cgaattcgat accctgaccg atacggttcg
     1621 ggccgccgcc gagaaccatg attttttcac ggtcggtaga cggattcgct tcgcactctt
     1681 cttcataagt ggagtacatg taagcggtgt cggtggcgaa ctctgccgca caggtatcca
     1741 cgcgcttata aaccgggtgc aggtcatact ggtcgcgcag cttgcggatt tccgcttcgc
     1801 gcacgcccgc cagttttgcc agacgcgcat cggcaaagcc tttgcgtttc agctggcgca
     1861 ggaattcagc gttcaggcca gtgatgccca cttccgctac tttctcttcc agacgcacca
     1921 gttcttcaat ctgtaccagg aaccagcggt caatattggt caggttgaac acgccgtcca
     1981 cagacaggcc cgcacggaag gcatcggcga tgtaccagat acgctctgcg ccagcatctt
     2041 tcagttcgcg acggattttg gttaacgctt ccgggtcatc gaggctcact ttcgggtcga
     2101 atccagtcgc accgacttcc aggccgcgca gcgctttttg cagggattcc tgctgcgtgc
     2161 gaccaatcgc catcacttcg ccaaccgatt tcatctgagt ggtcagacgg tcgttagcac
     2221 cagcgaattt ttcgaagttg aagcgaggaa ttttagtaac cacatagtcg atggacggct
     2281 cgaaggaggc cggagtacgt ccgccagtga tgtcgttcat cagttcgtcg agggtgtaac
     2341 ccaccgccag tttcgccgcc actttagcaa tcgggaaacc ggtcgctttc gacgccagcg
     2401 ccgaagaacg ggacacgcgt gggttcattt cgataacaat caggcgaccg tttttcgggt
     2461 tcaccgcaaa ctggacattg gaaccaccgg tttcaacacc gatttcacgc agcaccgcca
     2521 tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag cgtttgggct ggcgcgacag
     2581 tgatggagtc accggtatgg atgcccatcg catcaaagtt ttcgatagag cagacgatga
     2641 tgcagttgtc gtttttatca cgcaccactt ccatctcgta ctctttccag ccgatcagcg
     2701 actcatcaat cagcagctct ttggttgggg agagatccag accgcgggcg caaatttctt
     2761 caaactcttc gcggttataa gcgataccgc cgccgctacc gcccatggta aaggatgggc
     2821 gaataatgca cgggaagccc acgtcagcgg caaccgccag cgcttcttcc atcgtgtgtg
     2881 cgataccgga acgcgcggtt tccagaccaa ttttcttcat cgctacgtcg aaacgacggc
     2941 ggtcttctgc tttatcaatc gcatcggcgg tcgcaccaat catagtcacg ccgaactctt
     3001 ccaacacgcc ctgacgctcc agctccagag cgcagttcag cgccgtctga ccgcccatcg
     3061 ttggcagcac cgcgtccggg cgctcttttt caataatctt gcgtactact tcccagtgaa
     3121 tcggctcgat gtaggtcgca tcggccattt ccgggtcggt catgatggtc gccgggttgg
     3181 agttcaccag aatgacgcgg taaccctctt cgcgcagggc tttacatgct tgcgcgccag
     3241 agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc cgcacccaga atcaggatac
     3301 tttttatatc tgtacgtttt ggcatggctc ttttactcct gattacttag cggttttacg
     3361 gtactgctca attaactcga taaagtggtc gaacaacggc gctgcgtcgt gtggcccagg
     3421 gctggcttcc gggtgaccct ggaagctgaa cgccggttta tcagtgcgat gaatgccctg
     3481 taacgtaccg tcgaacaggg atttatgcgt gacacgcagg tttgcaggta atgttgcttc
     3541 atccaccgca aaaccgtggt tctgggcggt gatcatcacc acgtttttct ccacatcttt
     3601 aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg acagtcttcg caccgctcgc
     3661 cagcgccagc agctgatgac cgaggcagat gccgaatacc ggaatatcgg tttcgaggaa
     3721 tttctggatg gcggtaatgg cgtaatcgca cggggccggg tcgccaggac cgttggagag
     3781 gaagatgccg tctggattca ttttcagcac atcttccgca gaagtttgcg ccggaacgat
     3841 ggtcaggcga cagcctctat ccaccagcat ccgcaggatg ttgcgcttgg caccaaaatc
     3901 ataagccacg acgtggaacg gcagctcgtc ttcttttttc gcttctggca ggccgccggt
     3961 caacgtccag ctcccttgtg tccagctata ggcttctgcg gtggtcactt cttttgccag
     4021 atccatgcca ttcagacctg ggaacgcgcg ggctttttct aacgccaacg ccgcatccgg
     4081 gttatcgcct gcgataatgc agccattctg tgcgcctttc tcgcgcagta aacgcgtcag
     4141 cttacgggta tcgatatcgg caatcgccac gatgttatgg cgcttcaggt aagaagagag
     4201 gtcttcggta ttacggaagt tgctggcaat cagcggcagg tcgcgaatca ccagaccttg
     4261 tgcatgtacc tgagaagatt cttcatcggc gtcattagtg ccgacattgc caatatgggg
     4321 ataagtaaga gtaacgattt gacgagaata ggaaggatca gtgaggattt cttgataacc
     4381 ggtcattgaa gtattgaaaa cgacttcccc aaccgccgaa cctgttgccc ctatggcccg
     4441 accgtgaaac tgggttccgt cttccagaac caatagcgct gacttaatca aaacaccctc
     4501 cagagaatat tcactcactt tatttgcata ttaattcaca atgatgacgt aaatcaatgc
     4561 aaatctgctt accgacgaat ttctggcaaa cggcggcatt ctggagatat gggcgctaaa
     4621 agtcaactta atggcgggat tttttatatt attttgtggc tcatgcatgt tttacaggca
     4681 ttaagcgaca aaaagatcat ataacctgtt ctggaaaacg cttgcacagc aagaagattg
     4741 catgaaataa aagcgagcag ggaaaagtgg accaaatggt caaattttac attaaaacaa
     4801 caaaaacaga agacaaaaca tgtaattttt ggcacttata gacaatttaa aaacataaca
     4861 ctaaaaataa aagggccaaa attaaagccc tttataatca ataaattaaa tgtgttattt
     4921 ttgcaccata acaaatattt tgtggttata aactattgag atcaagcaca tctcgcatat
     4981 caaaaagacc actttcctta ccactcaacc acaaagccga tcttaccgcg ccgttagcaa
     5041 atgtcatgcg gctggacgcc ttatgggtga tctccagacg ctcgccaata tcggcaaaca
     5101 tcgcggtatg ttcgccaacg atgtcacctg cacgcacggt ggcaaaacca atggtgccag
     5161 gcacacgttc accggtgtgg ctttcacgac tgtagaccgc gcaatctttc agatctttat
     5221 caagggcgtg ggcgatcgcc tctcccattg ccagtgcggt gcctgacggc gcatcaactt
     5281 tatgtctatg atgtgcttca ataatttcga tatcggtgta gtcacccatc actttggctg
     5341 ctttctccag cagcttaagc atgacgttaa cgccaacgct aaaattggcg gcaaagacaa
     5401 tcgcaatatc ggcggcagcg tcacgaatcg cctgtttacc ggcttcgtca aaccccgtag
     5461 tgccgatcac catccctttg ccatgctggc gacaaaaagc gagatggttc agcgtacctt
     5521 ccggacgggt aaaatctata aacacatcaa aatcatcttt taccgcatcg aggctgcttt
     5581 gcacggtaac gcctgttttc cctgcaccag ccagctcacc ggcgtcgctg cccagtaaag
     5641 aagatccttc gcgctccagc gccgcgccca gctgcacgcc ttctaatgcc agcgccgcct
     5701 gaatcaactg gcggcccata cgccccccgg ctcccgcgat ggcaacgcgg atgtttgcat
     5761 catgcatagc tattctcttt tgttaatttg catagaccgt tttcagagta accagcgcat
     5821 cgctacgccg ccagccaaaa acgctgataa ttagaaatta atgataaaca gggagggata
     5881 tcagtgaaag gcatgagtcg atgaatgact gcatgccgat aacatgtgac aggttacgac
     5941 gccagagcca gcacctcagc cacccactgc tggaagcctt tcacatccag atccaatgcc
     6001 acctttacat tggctggttt gcccaggcaa ccgtcgatat caaccaccgt cgtgcctgag
     6061 gtaaattcgc cctgagtttc cactgccaca aaacacggtt tgagagtgaa caattccggg
     6121 cgcaccagcc aggcaatagc gcaaagatcg tgcatacgca gtccactttg catactgccg
     6181 ctacggtagt ggctaaacag ggcgtggagc atttttccgg tgcgattcaa ctccggcaaa
     6241 gtagcgagat agtcaggggt taatattgcc tgattggtga catccagacc gctcatgacg
     6301 atttcgatgc cgctacggaa gacacaggca gccgcttctg gatcggcagc gatattaaac
     6361 tcggcgtttg gtgtgcagtt gccgcgacca gcagaaccgc ccatgatcac cagacgacga
     6421 atatacggtt tacattccgg gcattgtgaa agtaacagcg caatattggt taacgggccg
     6481 atggccacca gggtaacggg ctctggtgca cgcatcaggg catcccgaat cgccagaaac
     6541 gctggtatcc cgagcggctt tcggttgtgc tcaacaaagt cgtagccagc cattcccgat
     6601 tcgccgtgca cagatgccgc atcacgcggt gcgcgtacca gcggcacagc ggctccctgg
     6661 gcgagcggaa tctccgcatt ccagaaatgc agcagttgca gggcgttgcg ggtggttttc
     6721 tcaaccgaga cattacccgc gacggtggtc atcagttgca ggtcgagttc gggtgcaaaa
     6781 atcgcggcgg caatggcgac ggcatcgtca atgccggggt cagtatcgag gaagataggt
     6841 aaatgcatgt tttctccata aaaaatgccg gtaacaagac cggcattttc gcataactta
     6901 ggctgctaat gacttaatcg acttcacgaa tatcgacacg cagctctttc ggcacttcga
     6961 aaacaatgtt ttcttcacgg ccttccagcg gaatggcttc accaccgccc agctgctgca
     7021 aacgtgccac cacattctgc accagaatat ccggagccga tgcgcccgca gtcacgccga
     7081 cgcatttaac ctctttcacc cactcttcct ggatgtcttt cgcatcgtca atcaaaaacg
     7141 cgcgtttgcc catacgctgg gccagctccg ccagacggtt ggagttggag gagtttttcg
     7201 aaccgaccac caacacaact tccgcctgtt ctgccagggc gcgtaccgct tcctgacggt
     7261 tagtcgtggc gtagcagatg tcatctttgc gcggaccgac aattttcggg aagcgtttac
     7321 gcagcgcgtc gatcacatca gacgtgtcat ccaccgacag cgtggtctgg gtcataaagg
     7381 agagcttctc ttcgtttttg accgtcagtt tccacacatc gtccggcgat tcgaccagat
     7441 acattccccc ttccgggtta ctgtactggc ccattgtccc ttccacttcc gggtgcccgg
     7501 cgtgaccgat gagaatagat tcttcgccac ggcgactggc gcgggcgact tccatatgca
     7561 ctttggtcac cagcggacag gtcgcgtcaa ataccgtcaa atcgcgactt tttgcttcgt
     7621 tacgtaccgc ctgagaaaca ccgtgtgcgg agaaaatcag gatcgcgccg tccggtactt
     7681 cgctaatctg ctcaataaag atagccccac gctcacgcaa gctatcgacc acatagcggt
     7741 tatgtaccac ttcgtgacgg acatatatcg gtgcgccgta aatggccagc gcgttttcaa
     7801 caatgctgat agcgcggtct accccggcac aaaaaccacg cgggttggcc aacaggatct
     7861 gcatgttacg cctccaatgc cggatcgatt tccagcactt caatatcaaa atgaacggtc
     7921 tgcccggcca gaggatggtt gaaatcaacg gtaatggagt cgccgttaat ttcgcggatc
     7981 acgccaggca tctcactgcc atccattgcg gtaaaaagca tgattgcgcc aatttctggc
     8041 tcgcctgcat ccataaattc acggcgggag aagtactgaa tcaggtccgg tgacggcacg
     8101 ccaaacgccg catctggctc caacgagaag gtggttttat cgcccacttt cagccccaac
     8161 aggtgttgct ccagcccttc agaaagagaa gcatcaccca ggcggaacag cgccggttta
     8221 ccgttgttgc gggtagactc ggcggtggtg ccatcgtcga gttttagcgt gaagtgcacc
     8281 aggacggcgc tattgctctg tacagattca gacatgcagg tttgctctta tatttgagga
     8341 tttttgccgg atgcggcgta aacgccttat ccggcagggt ttattattgt tttttcgctc
     8401 tagaaggcaa aaaaccttcc agcacaatca gtgccgcacc gacacagatg gcagtatcgg
     8461 caaggttgaa ggtggcgaag tgccagtcgc cgacgtagaa gtcgatcata tcgacaacga
     8521 agccgtgcca caggcggtcg aacaggttgc ccagcgcgcc gccaataatc agcgcgtaag
     8581 cgatattgtt tagcttctgc gtggccttcg agcgatacat catcactgcc aggatcacgc
     8641 taataccaat cgcaataccg gcaaagaacc aacgctgcca gccgccgcta tcggcaagga
     8701 aactaaacgc cgcgccatag ttacgcgcat aatgcagatt aagcgacggg aacagcggga
     8761 ccgtatcccc cagagcaaag ttctggagga tcaggtattt gctgcccaga tcgataatca
     8821 gcacgactac caccagccac agccagcgta gccctgttga acagatcgat tgactcatca
     8881 ggcaaactta cgtttttcac cgtcaccggc gacgttgctg acacagcggc cgcagatttc
     8941 tgcgtgttcc gccaccttgc cgacatcctg ggtgtagtgc cagcagcgtg ggcacttctc
     9001 accttcggct ttactcaacg cgactttcag ccctttgagt acttcgctct gctgagcatc
     9061 agcaggtgcg tcgtgatagt ctgcaacggt agcgccggag gtcaacagga caaatcgtaa
     9121 ttcatcgccc agcgcggtca gtttcgccgc cagttccggt tctgcataca aggttactgc
     9181 cgcttccagc gagccaccca ctttcttgtc ggcacgcgct tgctcaatga ctttgttcac
     9241 ttcgccacgc actttcaaca gctcgtccca gaacgcatcg ttcatcgctt cgctgtctgc
     9301 caggccaaac aggccttcgt accactcacc ggtgaagacg tatttttcac gttcgcccgg
     9361 caggtagccc cacacttcat cagcggtgaa ggagaggatt ggtgccatcc agcgcaccag
     9421 cgcttctgcg atgtgataca gcgcagtctg gcagctacga cgcgccacac tgtccgcttt
     9481 ggcggtgtac tgacggtctt tgatgatgtc gaggtagaag gaacccatct caacggagca
     9541 gaagcgcatc agacgctgta ccacttcgtg gaaatcgtat gcttcgtacg ccttgaggat
     9601 gtcttcctgt gccgctttcg cacaacctac ggcccagcga tccagtacca ccatctcttc
     9661 cggtttcacc atatcttttg ctggatcaaa accgttcagg tttgccagca ggaagcgcgc
     9721 ggtgttacgg atacgacgat agctatcggc agcacgtttc aggatctcgt cagaaacggc
     9781 catttcaccg gtgtagtcgg ttgatgccac ccacagacgc agaatatccg cgcccagttt
     9841 gttcatcaca tcctgcggcg aaacggtatt gccgatggat ttagacatct tgcggccctg
     9901 accatccacg gtaaagccgt gggtcagtac ctgacgatac ggcgctttgc ctttcatcgc
     9961 ggtggagatc attagggaag acatgaacca accacggtgc tggtcagaac cttccagata
    10021 catgtccgct gcgtgaccgg caaattccgg acgcacgtca acaacagaag agtgggtaga
    10081 tccggagtca aaccatacat ccaatgtgtc cggcactttc acgtactgat cagcttcatc
    10141 gccgaggatc tctttcgcat caagatccca ccacgcctgg atgccgtcaa cttcaacgcg
    10201 ttttgccact tcttccatta gttcgagagt acgcggatgc agctcttccg tgtctttgtg
    10261 cacgaacagt gacatcggta cgccccaggt gcgctgacgg gagatacacc agtcaggacg
    10321 gttagcaacc atcgactcga tacgcgcctg gccccagtcc gggatccact gcacgccttt
    10381 gatctctttc agtgactgcg cacgcagacc tttctgatcc atgctgacga accactgcgg
    10441 cgtcgcgcgg aagatgatcg gcgttttgtg acgccagcag cacggatagc tgtgctgcat
    10501 tttctcaacg tgcagcagcg cgcctttttc ctgcagcagc gcaacgacga tgtcgttcgc
    10561 tttgaagacg ttcacgccat ccagcgtcgg ataagtgccc ggcagataag tgccgtccgg
    10621 gccaaccggg ttagcggttt ccaggccgta tttctgaccg atcacatagt cgtccgggcc
    10681 gtggccaggc gcggtgtgaa cggcaccggt accggcatcc agggtaacgt gatcgccgag
    10741 gattgccgga acgtcgaagc ccataaacgg atgggtaaag cgcagcaact caagctccgc
    10801 accttttacc gtgccgagaa tggtgtaatc ggtcacgccg atacgctgca ttacgctttc
    10861 aaccagatct ttcgccagaa tcacggcctg accgtcgatc tgcaccagcg catagtcgaa
    10921 atcaggtgca atagagattg cgcggttcgc aggcagagtc cacggcgtag tggtccagat
    10981 caccagcgag attgggccgt taacgttgct tacggcaaat tttgctttca gtgcatcctg
    11041 atcgaccgcc tgaaaagcaa cgtcgatgga cggagaagtt ttgtcgtaat actcaacttc
    11101 cgcttccgcc agcgcagaac ggcagtcaac gcaccagtgt accggcttcg cgcctttgtg
    11161 caggtgaccg ttgccgatga ttttgcccag cgcgcggatg atgttggctt cagttttgaa
    11221 gtccatggtc aggtacgggt gcgaccagtc gcccagcacg cccagacgga taaagtcttt
    11281 gcgttgaccg tcaacctggg tcgccgcgta ttcgcggcac ttggcgcgga actcggcggc
    11341 ggtgaatttc tcacccggct taccgtattc ttgctctact ttcagctcga tcggcagacc
    11401 gtggcagtcc cagccaggca catacggcga gtcataaccg gaaagccctt tggacttcac
    11461 gataatgtct ttcagaatct tgttaaccga gtgaccaata tgaatgctgc cattcgcata
    11521 aggagggcca tcatgcagaa tgaaggtttt tttgcctttt ttagccgcac ggatgatgcc
    11581 gtacagatca tcatcagtcc aacgcgccag cattcccggt tcgcgcttgg cgagatcgcc
    11641 acgcatcggg aaccctgttt ccggcaaatt cagggttgat ttatagtcac tcatcagatt
    11701 ctcggttccg tgtttcggtt tgattacata acaggcttaa gccggttttg ttagcccaaa
    11761 aaattcgcgg gcggttaatt catcacgcgc aatctgcgct ttcagttcgt ccagcgacgc
    11821 aaatcgctgc tcattgcgta tttttttacg cagcactact tgtatatggc gaccgtaaag
    11881 gtccattgca acatctaaca aatgcacttc cagctgctgg cgaataccgg caaccgttgg
    11941 gcgtgttccg atgtttgcca cgccgggtaa cggcttttca ccgaggccca gcacttctac
    12001 cacataaacc cctttcaccg gggaaacctg acggcgtagc ggtacattcg ccgtcgggaa
    12061 acctatagtg cgccctaatt catcaccgtg gactacacgc ccggagatgg caaacgggtg
    12121 ccccagtaaa ctttctgcca gagccagatt gtcatccgcc agcgcctgac gcacagcggt
    12181 gctgctaata cgcacgccac cttcgcaaaa agtttgcgta ctagtgatat cgaagccgta
    12241 ttccatgcca gctttctgta ataacaagaa atcgccttca cgaccagcgc caaagcggaa
    12301 atcatcccct acggcaagaa attttacgcg caaatgcttc accagaagat cgctgatgaa
    12361 attttgcgcg gttaacgccg cgaaacgcct gtcgaaacgc acgcacagca cgtaatcaac
    12421 gccacactct gcaaggtaac gcagtttttc ccgcagccgg gtcagtcttg ccggggcttt
    12481 atcggtagca aacagttcca gtggttgagg ttcaaaaagc atcaccatca ccggtaagtt
    12541 gcgcttgcgc ccttcttcct gcaagccctg taacagcgcg cgatgaccgc gatgcacgcc
    12601 gtcgaaatta ccaatagtca gcacacaccc ttcttgcggg gcctggctga gattatgtat
    12661 gccgcgtatc agcttcatgt ctggctcaaa acagtgaaaa tcgtccgagt ataccttgta
    12721 cagcggtcaa ggttaaccgg cgattgagta ccgaaatcag aaagaagtgg cttttcattg
    12781 ccatggcgca aatcacggga agaaagtgac cgcctgctgc aatttttatc gcggaaaagc
    12841 tgtattcaca ccccgcaagc tggtagaatc ctgcgccatc actacgtaac gagtgccggc
    12901 acattaacgg cgcttatttg cacaaatcca ttgacaaaag aaggctaaaa gggcatattc
    12961 ctcggccttt gaattgtcca tatagaacac atttgggagt tggaccttgg ctaatatcaa
    13021 atcagctaag aagcgcgcca ttcagtctga aaaggctcgt aagcacaacg caagccgtcg
    13081 ctctatgatg cgtactttca tcaagaaagt atacgcagct atcaaagctg gcgacaaagc
    13141 tgctgcacag aaagcattta acgaaatgca accgatcgtg gaccgtcagg ctgctaaagg
    13201 tctgatccac aaaaacaaag ctgcacgtca taaggctaac ctgactgcac agatcaacaa
    13261 actggcttaa tcgccaattt gctgaagctt tgtgaaaaag cccgcgcaag cgggtttttt
    13321 tatgcctgct gcttttatga tgcgtgagag aacacactac cggttgccag atagtcaaaa
    13381 gatgctggct tttgcagaca cgccgttaca ctttctgaac tcttcgtggc ttaagaatcg
    13441 tagaaccatc ggctgataca ttctgaacaa gaatatctac t
//
LOCUS       DAJVOY010000103        13435 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVOY010000103 DAJVOY010000000
VERSION     DAJVOY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.103"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            127..942
                     /gene="mepH"
                     /locus_tag="OQL90_002664"
     CDS             127..942
                     /gene="mepH"
                     /locus_tag="OQL90_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1369394.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1070..1651
                     /gene="sodB"
                     /locus_tag="OQL90_002665"
     CDS             1070..1651
                     /gene="sodB"
                     /locus_tag="OQL90_002665"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1369395.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1797..2966)
                     /locus_tag="OQL90_002666"
     CDS             complement(1797..2966)
                     /locus_tag="OQL90_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1369396.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3132..3221)
                     /gene="ynhF"
                     /locus_tag="OQL90_002667"
     CDS             complement(3132..3221)
                     /gene="ynhF"
                     /locus_tag="OQL90_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1369397.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3520..4545
                     /gene="purR"
                     /locus_tag="OQL90_002668"
     CDS             3520..4545
                     /gene="purR"
                     /locus_tag="OQL90_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1369398.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4542..5474)
                     /locus_tag="OQL90_002669"
     CDS             complement(4542..5474)
                     /locus_tag="OQL90_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1369399.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5587..6798
                     /locus_tag="OQL90_002670"
     CDS             5587..6798
                     /locus_tag="OQL90_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1369400.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7089..8237
                     /gene="cfa"
                     /locus_tag="OQL90_002671"
     CDS             7089..8237
                     /gene="cfa"
                     /locus_tag="OQL90_002671"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1369401.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8277..8918)
                     /gene="ribE"
                     /locus_tag="OQL90_002672"
     CDS             complement(8277..8918)
                     /gene="ribE"
                     /locus_tag="OQL90_002672"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1369402.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9133..10506
                     /gene="mdtK"
                     /locus_tag="OQL90_002673"
     CDS             9133..10506
                     /gene="mdtK"
                     /locus_tag="OQL90_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1369403.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10547..11803)
                     /locus_tag="OQL90_002674"
     CDS             complement(10547..11803)
                     /locus_tag="OQL90_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1369404.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12111..12187
                     /locus_tag="OQL90_002675"
     tRNA            12111..12187
                     /locus_tag="OQL90_002675"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12145..12147,aa:Val,seq:gac)
     gene            12192..12268
                     /locus_tag="OQL90_002676"
     tRNA            12192..12268
                     /locus_tag="OQL90_002676"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12226..12228,aa:Val,seq:gac)
     gene            12376..12681
                     /locus_tag="OQL90_002677"
     CDS             12376..12681
                     /locus_tag="OQL90_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1369405.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12807..>13435
                     /locus_tag="OQL90_002678"
     CDS             12807..>13435
                     /locus_tag="OQL90_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCS1369406.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAV"
BASE COUNT         3313 a         3464 c         3201 g         3457 t
ORIGIN      
        1 tcgccaacga aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat
       61 tagaatcatc aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga
      121 cgctcagtgg cgcagataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc
      181 ctgcctttaa cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact
      241 catatcacca aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa
      301 acagcgaaaa aagccgccag taaaagtacg accaaaagca aaaccgcttc ttccgttaaa
      361 aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa
      421 acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt
      481 gtgaaagtca aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa
      541 aaagctacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg
      601 ggtggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa
      661 gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca
      721 gcgccaatcg aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga
      781 cgcggcacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg
      841 cgcacaggtc aggaaattca aatcacctct ctcagtgaag actactggca gcgccactat
      901 gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta
      961 cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc
     1021 acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga
     1081 attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat
     1141 cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa
     1201 aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt
     1261 attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa
     1321 cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt
     1381 tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac
     1441 ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac
     1501 tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta
     1561 catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg
     1621 ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga
     1681 tgcgcttcgt ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa
     1741 tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag
     1801 ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat
     1861 gccagtcccg cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa
     1921 attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg
     1981 actgatgaag acagacctgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc
     2041 ggtacgaccg caaaggctgc tgcgccccac accaccatgc taatagctgc gccgaactca
     2101 ttgcgggcca ggaacgggat tgccagcata atcaccatca acaacaacaa aaagcctttc
     2161 aacgtgccgt taactgaacg atctgccagt ttgccgccgg gatagttacc gatagagaat
     2221 ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt
     2281 tgcagtaccg gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc
     2341 gtcagcaatg cagacaacac ctgcggacgc attaataccg ccagctcttt tttcacttca
     2401 ggtcgtgccc ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact
     2461 cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc
     2521 gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg
     2581 ctggcctgtt tatgttttgg ctccacgctt gcggccacga ctgaacccaa accaaaaaat
     2641 gctccgtgat tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg
     2701 atggcggaaa gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg
     2761 cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca
     2821 taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg
     2881 ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccaga
     2941 gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc attacgcgat
     3001 attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata acaaaaagag
     3061 aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga acgcgctccc
     3121 tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ccacgatcaa accgaggacg
     3181 ataatcgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc tcctttttta
     3241 ttaccacaca aatagtgata ttacgcattt ttacacactg tgatgaaaaa atctcccgtc
     3301 atttataatg ataagtgttt ttaccacttc cccttttcgg caagatcggc caaaattcca
     3361 cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg tcgtttttcc
     3421 ggcgtaccgc aacacttttg ttgtgcgtaa ggcgtgtaaa ggcaaacgtt taccttgcga
     3481 ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat aaaagatgta
     3541 gcgaaacgag caaacgtttc cactacaact gtgtcacacg taatcaacaa aacacgtttc
     3601 gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca ctactcccct
     3661 agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt gctggcgacc
     3721 agcagcgaag cggcctattt tgccgagatc attgaagcgg ttgaaaaaaa ttgcttccag
     3781 aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat
     3841 ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca
     3901 gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt gatggactgg
     3961 ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga aggcggctac
     4021 atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg
     4081 ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat ggaagaagcg
     4141 atgatcaagg tgccggaaag ctggattgtt cagggtgact ttgaacccga atctggttat
     4201 cgcgccatgc agcaaatact gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc
     4261 gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggtctgcg cgtcccgcag
     4321 gatgtttcgc tgatcggtta tgataacgtg cgcaacgccc gctattttac gccggcgctg
     4381 accacgatcc accagccaaa agattcgctg ggtgaaacag cgttcaacat gctgttggat
     4441 cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg cttgattgaa
     4501 cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc cgttgcggga
     4561 gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgcc gagatactca
     4621 agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca acatgccgaa
     4681 tccgggaagg ggttttccaa ttcaagtgct acccacttcc cctcattaag ccacggtttg
     4741 gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt tgccgatgat
     4801 tcccagtcag gcacgacaac tcttttttgg ttatccagta accaggtaat acgtttaggt
     4861 agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg
     4921 aatgggccat ccatcaacgc cagcgggtgg tggctggcaa caacgcaact ccagcttagc
     4981 atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc
     5041 gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac ttcctgaaag
     5101 acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt
     5161 tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc gttcgctatc
     5221 tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc ttctttgaga
     5281 aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa gagcggcacc
     5341 gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac gcgatgcagc
     5401 tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac ttcgagtgaa
     5461 tattctgacc acatagtctg cctgcaaaat ttttgaaatc agtcatcaaa tattaccgtt
     5521 tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga gatttagaga
     5581 atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag cgtactcggt
     5641 tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga cctgcaaacg
     5701 cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc cgcagcccag
     5761 cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg
     5821 acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc tacgctgctg
     5881 gtattgcgtt ttgtacaggc tgtgggtgtc tgcgccgcgg cggttatctg gcaagcgtta
     5941 gtgacggatt attatccttc acagaaagtt aaccgaattt ttgcgaccat catgccgctg
     6001 gtgggtctat ctccggccct ggctcctctg ttaggaagct ggctgctggt ccatttttcc
     6061 tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct gcctattttc
     6121 tggctcaaac ccacgacgaa ggccggtaac aatagtcagg atggtctgac ctttaccgac
     6181 ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcggcctg ttcagccagt
     6241 ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta cagcccggca
     6301 gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg ttatggctgt
     6361 cgcgccgcgc tgcagaaatg gcaaggcaag cagttattac cgtggttgct ggtgctgttt
     6421 gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc gctggtcgaa
     6481 atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc tattgttgtc
     6541 gcccaggcgc tgcgtccctt cccacacgca actggtcgcg ccgcagcgtt gcagaacact
     6601 ctacaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat tagtatcagc
     6661 acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt
     6721 tacatgatgc aacgttgtga agaagctggc tgccagaatc atggcaatgc cgaagtcgct
     6781 catagcgaat cacactgatc tatatcgata tacttatact taggctgcta acaaaatttt
     6841 gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct acgataaata
     6901 ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat ttcggcaagg
     6961 gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt ctgcgagatt
     7021 tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg accagtgatg
     7081 gagaaactat gagttcatcg tgtatagaag aagtcagcgt accggatgac aactggtacc
     7141 gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct gccccggcgg
     7201 atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaagaaggc tctttggggt
     7261 taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg ttttttagca
     7321 aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac acgctgcgta
     7381 ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata gtcggcaaag
     7441 agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc atgcaatatt
     7501 cctgtgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg aagctcaaaa
     7561 tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt ggctgcggct
     7621 ggggcggact ggcacactac atggcatcta attatgacgt aagcatggtg ggcgtcacca
     7681 tttctgccga acagcaaaaa atggctcagg aacgctgtga aggcctggat gtcaccattt
     7741 tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct gtggggatgt
     7801 tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat cgtaatttga
     7861 aaccggaagg catattcctg ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg
     7921 atccctggat taataaatat atttttccga acggttgcct gccctctgta cgccagattg
     7981 ctcagtccag cgaaccccac tttgtgatgg aagactggca taacttcggt gctgattacg
     8041 atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa attgcggata
     8101 actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc gcaggtgctt
     8161 tccgcgcccg tgatattcag ctctggcagg tcgtgttctc acgcggtgtt gaaaacggcc
     8221 ttcgagtcgc tcgctaaagg ctattctatc gccccctctc cgggggcgat ttcagatcag
     8281 gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg ttctaccgta
     8341 tctaccactg cctgagtttg tggatcaatt tcaatgttga cgcgtgcgcc gagttttttc
     8401 ttcccaagag tcgtgcgttc cagagtttcc ggaattaaat ggacgcaaaa acgcgttggc
     8461 gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt gtacaggata
     8521 tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt ttccgaggtt
     8581 aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg tccgccaatt
     8641 tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt taaatcgcca
     8701 agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt gccgttaatt
     8761 tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc caggccgtcc
     8821 agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc gtcaatcgac
     8881 accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc tgaaatcagt
     8941 taagacattc tgttcagcac aatagcaggt ggcaaacgcc cttaccagtg aaggggtaag
     9001 aatgactatt ttttcactgg agaattaata aatcctcgct acaatagact gaatttcccc
     9061 tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata actacaaata
     9121 aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc attagcaatc
     9181 ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg
     9241 ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat ctggcttccg
     9301 gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc gcaattaaat
     9361 ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg gctggcaggt
     9421 tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat ccgctccatg
     9481 gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg
     9541 ggcgcgccgg gctatctgtt cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa
     9601 accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacattcc ggtgaactat
     9661 atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg tggcgtggct
     9721 actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa acgcgcccgc
     9781 tccatgcgcg atattcgtaa cgaaaaaggc accgcgaaac ccgatcctgc ggttatgaaa
     9841 cgactgattc aactcggttt accgattgcg ctggcactat tctttgaagt gacactgttt
     9901 gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg acaccagatt
     9961 gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc agcggtaact
    10021 atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg
    10081 accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac ggtttcactc
    10141 cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat
    10201 ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat tggcagtggg
    10261 attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac agcttactgg
    10321 gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt tgaacctatg
    10381 gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc cattatgatg
    10441 atgttgcgta tgcggttcct gcaacgtctg ccgtcagtta tcattctgca acgagcatcc
    10501 cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac gcaggctgga
    10561 gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttgcac agagtttgcc
    10621 gactggcatc tgttatctca acgtgtatat caccggtaac ttcccctttc acattgatga
    10681 agtcaggtga gatttctttc gcactggttt gcaggcaaat cgctccctct ccgcgcaggc
    10741 gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt tttccgccat
    10801 tcagattgat gttaccaaca ataaaagtga caccgtcacc ttttaccgta ctgccgtcga
    10861 gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagatct gcgccatagc
    10921 caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca accggtgcgc
    10981 gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg atcactgttc
    11041 caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccga ccacccacat
    11101 tcacggacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc gtccccagac
    11161 gataaatgga tccattctca acaatggtgt cagagtcttc accaccgaga ttattcatgg
    11221 tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg ccgccctgat
    11281 taatctcaca attgctggct tccccacctg gatacaccaa caattcacca ccttcatcta
    11341 ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg agaatgattt
    11401 tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc aacaaaccgc
    11461 aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta accgtagcga
    11521 gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca atggcaccgt
    11581 cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc ccaccggttg
    11641 ccactttcaa ccagccagcc cccgtgacta cggtattttc ggctcttccg ccagcttcaa
    11701 caatcaactc gccttcagta agttgcgtcg cgccaatgac ctcgccggtc ttaacaattt
    11761 gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat tcctttgctg
    11821 catctgtgtg cctttattgc tacctaaatg taaaggctac ggaggattta tccacgacag
    11881 atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat tgggttgtat
    11941 cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa tatagccaat
    12001 ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc
    12061 accttcccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag
    12121 ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag tccaattgaa
    12181 cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat ggtgggggtc
    12241 ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtcttct cctttttccc
    12301 tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt atgcgttatc
    12361 agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa tggcccattt
    12421 ggcgacgcaa tggctgagca gcttaaaccg cttgctgagt cgattaatca ggaacctggt
    12481 tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg gatctatctt
    12541 tttactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg acttaaaaat
    12601 ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact gagtcaaatc
    12661 aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc ttttcatccc
    12721 tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa cactaaattc
    12781 gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt gccgggccta
    12841 cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct atttctatct
    12901 tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa
    12961 tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg tatctcgaat
    13021 ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc
    13081 acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat caatttttac
    13141 gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg
    13201 ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag gatttaccca
    13261 gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat gaagaagaag
    13321 caaccagaac gtattttccc agtccgtggt caggcctgat ggaagcaaag gtcgatgcgt
    13381 gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg gcagt
//
LOCUS       DAJVOY010000104        13380 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVOY010000104 DAJVOY010000000
VERSION     DAJVOY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.104"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..1867)
                     /gene="speC"
                     /locus_tag="OQL90_002679"
                     /pseudo
     CDS             complement(1..1867)
                     /gene="speC"
                     /locus_tag="OQL90_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2265..2963
                     /locus_tag="OQL90_002680"
     CDS             2265..2963
                     /locus_tag="OQL90_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1369407.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3069..3144
                     /locus_tag="OQL90_002681"
     tRNA            3069..3144
                     /locus_tag="OQL90_002681"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3102..3104,aa:Phe,seq:gaa)
     gene            complement(3293..3829)
                     /gene="yghD"
                     /locus_tag="OQL90_002682"
     CDS             complement(3293..3829)
                     /gene="yghD"
                     /locus_tag="OQL90_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCS1369408.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3831..5009)
                     /gene="gspL"
                     /locus_tag="OQL90_002683"
     CDS             complement(3831..5009)
                     /gene="gspL"
                     /locus_tag="OQL90_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCS1369409.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5006..5983)
                     /gene="gspK"
                     /locus_tag="OQL90_002684"
     CDS             complement(5006..5983)
                     /gene="gspK"
                     /locus_tag="OQL90_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCS1369410.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5986..6585)
                     /gene="gspJ"
                     /locus_tag="OQL90_002685"
     CDS             complement(5986..6585)
                     /gene="gspJ"
                     /locus_tag="OQL90_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCS1369411.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6582..6953)
                     /gene="gspI"
                     /locus_tag="OQL90_002686"
     CDS             complement(6582..6953)
                     /gene="gspI"
                     /locus_tag="OQL90_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCS1369412.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7013..7513)
                     /gene="gspH"
                     /locus_tag="OQL90_002687"
     CDS             complement(7013..7513)
                     /gene="gspH"
                     /locus_tag="OQL90_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCS1369413.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7517..7972)
                     /gene="gspG"
                     /locus_tag="OQL90_002688"
     CDS             complement(7517..7972)
                     /gene="gspG"
                     /locus_tag="OQL90_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCS1369414.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLARTVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7989..9212)
                     /gene="gspF"
                     /locus_tag="OQL90_002689"
     CDS             complement(7989..9212)
                     /gene="gspF"
                     /locus_tag="OQL90_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCS1369415.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQRGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9212..10704)
                     /gene="gspE"
                     /locus_tag="OQL90_002690"
                     /pseudo
     CDS             complement(9212..10704)
                     /gene="gspE"
                     /locus_tag="OQL90_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10704..12764)
                     /gene="gspD"
                     /locus_tag="OQL90_002691"
                     /pseudo
     CDS             complement(10704..12764)
                     /gene="gspD"
                     /locus_tag="OQL90_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12794..13366)
                     /gene="gspC"
                     /locus_tag="OQL90_002692"
                     /pseudo
     CDS             complement(12794..13366)
                     /gene="gspC"
                     /locus_tag="OQL90_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
BASE COUNT         2990 a         3871 c         3451 g         3068 t
ORIGIN      
        1 agcgctatgc gcatcctggg ggttcatcac cactgacggg aaactctgct ggcggaacat
       61 cgctttttgt aggtctttga cgtcgaaact gacatacaga tcgtgcatct cctgacacaa
      121 ctggcgcaaa gtgtagtcgc gatagcgcac cggatatttg ttataaacgc tcggcaacac
      181 ctcagccagc ggcgagtcat cctcaatatg ctgttcaaac tgcgccaaca tctctaccag
      241 ttgtgccagc ttctcgtggc tttccgccgg agttaataag aacagaatgg agttgagatc
      301 gcacttctcc ggcacaatgc cgttctcacg cagatagtgc gccagaatcg tcgccggaac
      361 gccaaagtcg ctatattcgc cggtttcggc attgatacct ggtgtagtga gtaacagctt
      421 gcacggatca acaaaatact gatccgcggc atatccttca aagccgtgcc acttcgcccc
      481 cggctcaaaa ctgaaaaaac ggcggtcgct ggctaacact gatgtcggat aatcctgcca
      541 caatttgcca tcaacaacgg gcgggataaa cgggcggaac agcttacagc gcgcaagaat
      601 agccttgcgc gactcaatac ccaactcaac gcactcagcc cacagccgac gcccactctc
      661 cccttcatga attttggcgt taacatccag tgcagcaaac agcggataga aagggctggt
      721 agaagcatgg agcataaagg cgttattcaa ccgcttatgc gggcaaaaac gcgcctgtcc
      781 gcggatatgg ttatcttttt tatggatctg cgacgtctgt gagaatcccg cctgctgttt
      841 gtgcaccgac tgagtcacaa agatctccgg atcgttttcg ttaagttcta atagcagcgg
      901 cgagctatcc gccatcatcg ggataaactg ttcgtaaccg acccacgcgg aatcaaacag
      961 aatgtaatca cacagatgcc caacggtatc gatcacctga cgggcgttat agacagttcc
     1021 gtcataggtt cccagctgaa taatcgccag gcgaaacggg cgcggcagct cggctttttc
     1081 tggcgcaacg tcgcgaattt gctggcgcag atactcttca ttaaaacagt gcgcatcaat
     1141 accgccaatg aaaccaaacg ggttgcgtga agcttccaga tagaccggcg tcgcccccgc
     1201 ctgaatcagc gcgccgtgat gattcgactt atggttgtta cggtcgaaga gcaccagatc
     1261 gccacgcgtt aacagcgcat tcgtcaccac tttattcgct gccgatgtgc cgttcagcac
     1321 aaaataggtt ttatcggcat gaaagacttt ggctgcgaat ttctgcgcat ctttcgccaa
     1381 taagcagatc gcccaatttt acgtcagcgt tacacatatc ggcgcgaaag acgttctcac
     1441 caaagaaatc gtaaaaatgg cgtccggcag gatgcttttt aaaaaacgca ccatgttgat
     1501 gtccagggca agcaaaggtg ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa
     1561 acggtggcag caaattctct tcatactgac aggctgcgga ttccagctcc agccactgct
     1621 gctcgttgcc gttgattacc gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat
     1681 acaaaaacac cggtagatga aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac
     1741 tatccgcagc ggtaatgacg actgccacga cgtccgtaaa atcagtatct cccaacgcca
     1801 ccacgcgacg atgagaagaa agtcgggata ccagttcact actggcggca atattcattg
     1861 atttcataag cgcaaacccg tttcggggaa gtaagaatac cggacaaggt ggaaaaccct
     1921 gccccatgag atatgggtca aactggtcac cagctccgac cgccagacat cagtaaaagc
     1981 agaaacgctc tgattttact gttgtcctgc agtgagcgtg cgttaacttc accgcatgag
     2041 cagtaacata gaaagggaaa cgtttcgcgc gaaacggcga taagcgagag aatgtaagga
     2101 ggtggcgtgc atcgggcaaa ctccgtacag agaggagaaa attcgcgcaa tcatggcacc
     2161 tttcgctaaa gcgtgcaagc caggactttg cgaccagaca agccatcaac aaggcaaaaa
     2221 gttcataata agaaaatcaa acaatacagt taacaggaac tcttgtggtc atcggccctt
     2281 ttatcaacgc tagtgcagtc ttactgggtg gcgttctcgg cgcactgctc agccaacgct
     2341 taccggaacg tatccgcatc tccatgacat ctatttttgg tctggcatcg ctggggattg
     2401 gtattttact ggttgtgaaa tgcgccaacc ttccagcgat ggttttagct acgctacttg
     2461 gcgctctaat tggcgaaatt tgtttgctgg agaaaggtgt caatacagca gtcgccaaag
     2521 cacaaaatct gtttcgtcac tcacgtaaga agccaacgca tgaatctttt attcagaatt
     2581 atgtcgcgat tattgtcctg ttttgcgcca gcggcaccgg gatcttcggg gcgatgaacg
     2641 aagggatgac cggcgatccg agtattttaa tcgccaagtc atttcttgat ttctttacgg
     2701 cgatgatctt cgcctgctcg ctgggtattg cggtgtcggt aattagtatc ccattactga
     2761 tcatccagtt aacgctggcg tgggctgccg cgctgatatt accgctgacc acaccgtcga
     2821 tgatggcaga cttcagcgcc gtaggcggtt tattgctgct ggcaaccgga ttacgcatct
     2881 gtggcattaa aatgttcccg gtggtcaaca tgcttccagc actccttctg gcaatgccgc
     2941 tttccgccgc ctggtttgcc tgacaatgcg tgcaatagcg gcaaagtgat gatagattgt
     3001 gcagtctgca gtaaattgaa gaaatttgat tgacgagacg aggcgaatca ggtttaatgc
     3061 gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatccc cgtgtccttg
     3121 gttcgattcc gagtccgggc accactaatt cttaagaacc cgcccacaag gcgggttttt
     3181 gcttttggat ctgacaataa ccttcacgaa aaaagtttgc ttataaagtc tgggggaatt
     3241 actctcgcca cgttaacgag agtaatttta ttgatactaa tctcctgata ctttaccccc
     3301 gtccaaactc cagccgctgg acattcacca tcccagtctt ctcagcagca ctggcatcaa
     3361 tttgtgtcac ccgcagcgca tacttttcat ccagcgcgtt taaccagttc agcaggtcat
     3421 taaacaccac cggatctatc cagacctgaa tattctcccc acgctcggct atccgcttga
     3481 tgactaccga gtgcgcggaa gcactgtcac tgataacccg cgatacctgc gcaggcgttg
     3541 ttgtggcgga ttttcgcgcc gcaataatat ccggcgcggc gctcctcagt ctggtgttca
     3601 tcgccaccag ttgctgcaac attgtctcct gttgtactat ccgttcgctc aacggctgcc
     3661 agatgaggac gtaatatccg acgctaaaca ggaacaccac cgctgccagt aacatgccct
     3721 tttcacgcgg agaacgcccc gccaggtgtt gtgccagcca gtgttcgcca cggcttaact
     3781 ggcgttcacg ccattgctga aaatagtgaa taaatttatc gcgtaacatg ttatttcctc
     3841 cgcaacgtta cgccgccgga aaccgcatca ccctctttct gtaacgcgtc ctgttgcaca
     3901 acataatcgg ttgccagtgc actacgcagt ttgtcgaaac tggcaaagtt cgcggcccgt
     3961 agctggaggt gaagtatctg gcgtttttga tcaaaggtaa aaccacgcat ttcgatgtcg
     4021 gaaagtgacg ctgatttcag ggtgctggct atcgctgaca actcggcgag cagccgggta
     4081 tcgtcggcct gtgggcgata ttttttcagc gccatcgtca cctgagagcg taaattcaca
     4141 atccgcttct gctccgggaa caacgttaag aactgttcct ccgcctgggt gcggctttgc
     4201 gccacctgtt cgctgacgct ccataacgtc acgccccgct ccactgccag cgcaactaga
     4261 atcagcaata tcggcagaat catcacccgc cagcgcgccc actgttttcg gtagctgacg
     4321 cgaggctgcc acggccctgt cagcaggttc ccttccggtt cgccataagt ggtaacggcg
     4381 ggcagagctg taacggtcag gcgttcagcg tctgcgccag cccatgctga tagcttttcc
     4441 ggtgcaatgc cgactacggt tagcgaaagc ggtaaatcct gctcattgag ctgggcgcgg
     4501 aacatgaccg gagccagcgc ccgcccggcg ctccatcccc ggcattcatc aatgcggcag
     4561 ataacccgtt gcgcatcgcc agccataacc ccacaaggaa tggacatcca gtctggcgcg
     4621 acggtagcgc gggtgatgcc gttttcctgc aaccactgcg caatgttgcg catatgctgt
     4681 tggtgaatca ctgctacggt tgccagttgc tggtcgatat tcaacggggc gaaatgcagt
     4741 tcatcgatat cctggttcag ctcttcttcc agtaaggcgg gcagaatggt cggtatctgc
     4801 ttacgggcga catcaggcag ttcaacctgc cagacgctga tccattcgcc gggaatgtag
     4861 agtcgaatcg catcggtttg cagccattgc tggagctgtt cgttagcaac gtcagaccag
     4921 ataccgtgct ccacatcggg tgtacggcgc tgccaatgga tgggatcagc ggcgcaaagc
     4981 gggaaaaaaa tctcaaggat ggaactcact cactttctcc tgtctgatgc cagagaaccg
     5041 aaaagtgttg tgggcccatg cggacaatta acgagttcat cgtcagttca atctcattca
     5101 cggtgatatc tgaacgcagc cagaagtaat tgctgtccac gctcaggacg gtttttagct
     5161 gttttttagt acgctcatcg acgtcagcaa gtagcggctg tgcaagaaac tgatcgacat
     5221 cttcccagcc cttcgccgga cgttgttgta ataacgcccg cgcctgaacg gggcttaacc
     5281 acgggtcaaa cagcgcctca agaatcacac tttgcgtgac gtctaaagta ttgatgttga
     5341 tttgctggcg gatcatcggc agcgcacaga ccagcggttt cagtttttga taaagcccgg
     5401 cgtccattcc ctgtaccacg cgcatctcac tgatatcagc cagcggttga ttggcggcgt
     5461 agaacggcac cgaacgggcg agatactcgc tgtcttcacg gcccagacgt gtctgcacgc
     5521 tgcggtcctc gtcaataaac tcccacaggc tttcggctat cagttcggcc cgataagcag
     5581 gcacatccag gcgcgagatc agggcaatca actgttgtac cgcaagcgga cgcgacgccg
     5641 ttgtcggctg agcaagggca ttcaggttaa agcaagcctg tgcgtcacgc agggtgaccg
     5701 caatttgccc ctgcggcaga gggaagaaac gagggccgga tgcccacggc tgtgccagat
     5761 gcacgcgctt ttcatttttc aggctcagac tgagcgcgct cagcgccagg ctttccgcac
     5821 tggcgctgta ccacagcgcc tgctggtact cctgctggct gcgcgttcgc ccaagttgtt
     5881 gctgcattcg cccggaaagc gtgatggtta ccagcatcat aaccgccagc aataccagca
     5941 ccacgaccag tgccattccg cgttttggtg gtgaggtgat catgattatt gcggcccgcg
     6001 taacaaccag atgcgttcaa tctcgcccca ttgcggcgaa tgcagggtca tgcgtaccgc
     6061 cacagggatc gcctgtactg acgaccagct ctcctgccag cgcgtgccgt cgtagaactg
     6121 caaacggagc gaatcggccg gaatcaactt ttgcattgtc ggcttcacgc tgcctgcggc
     6181 atcggtcagc ggccaggcta accgttcgag ataaccgtca tgaatgcggt aaccgacggt
     6241 gagcagattg ctgcgcggca gacgcatcaa cgggttaacc acaccgccgc gcacaaaacg
     6301 cattccttca ctctcagacg ccagcacgcc agcgccagcc agtaaagctg gttcacgctg
     6361 gccctgatcg cctcttaccg gacgcggcat catttgggtc agatcgtggg tcagaaaact
     6421 catcgtttgc tgcatgaggt ttagtttttg atcgtgatcg gcgacggcgc tattcacgcg
     6481 cgtgacgccg ttagtcacct gctgcgccat cagtgccagc gaggcaaaaa tggcaattgc
     6541 caccaacatt tccagtaacg tgaaaccagc gcgagtcctt ctcactgttg gcctcccacg
     6601 gcgctaaacc aggcgcgtcg tgactgaatc accgacgaaa agtcttcgtg acggctgact
     6661 tcaatatcca ccgcctggag cagcgcgttg cctgtattca atgggattgt tcgccagtac
     6721 cagcggcttc ctgccatatc gctctcgccc tgatgcctga ccgcccgctg ctcacgggtc
     6781 atcaggtaga gcagcgcagt ctggttttca gctacccagc ccgctaccgt tttttcctca
     6841 agaacgtgct gattactcag cgcaccgctg gcaatctgta acaccgccgt ggcagccagc
     6901 gcaaaaatcg ccagcacgag catcacttcc agcaaggtaa atccgcgctt catggcatcc
     6961 tctcatcaca ttgattgagg gataacgcgc catcgtgtgc cagctttacc gctcaacatg
     7021 cgttttgcgc cgccgagtag aagcgcagcg taaacggcgt ggcgggttca aaaggcgaaa
     7081 aacggatctg cggcgtcttc tttttcgcct ctttttgcaa ctccagttca atatcgtgca
     7141 gcgtcaggcg acgacgttgc agctccagcg catattcctt ctgccagata tcacttccgg
     7201 gttgtaacag catctgcacc tgtttcggca ccgtaacctg tgccgataac cgggtcgcag
     7261 aaacgggcaa ccactgcccc tgacgacgct gcataaactg atagccaggc gggtcgatta
     7321 gcacaccgag tgtctgtcct tcaatcactg ccctgtcctt aaactgcgca aagcgagtca
     7381 gaaaatcctg tgccgctttt ttcgcgggcg gttcagaatc ggtcgcaaac gtctgtacca
     7441 cgcccgcact ggcaaggccg ataaggaaaa tcaccagcat gatttccaga agtgtgaatc
     7501 cgcgttcagg cactgcttac tgaaactcct gcaaattcca gttaccgata tctgcgccag
     7561 cgccttcccc gttttcctga ccatccgccc ccagggtata aacatcaaac agtccttttt
     7621 cacccgggct gagatactga taatcattgc cccacggatc ctttggcagt cgtttgatgt
     7681 atccaccagt acggtagttg cgggagtccg ccatattggc tggctgctgg atcagcgctt
     7741 ccagcccctg ttctgtggtc ggataacgcc cgttatccag tcggtacata tctagtgcgt
     7801 tttccagcgc cacgatatcg ctgatggctt tttgccgatt ggctttctct ttgttgccca
     7861 acaggttagg caccaccgtc cttgccagga cgccaagaat aacaatcacc accatcactt
     7921 ccagcagggt aaaacctgcc cgtggtttgt gtgtgcggga taacgaattc atgtgataac
     7981 tccgtaaatt acattccaac catattattc agttgcagca tcggctcgag gatggcgatg
     8041 acaataaaca gcaccacgcc cgccatcatc accaccagcg caggctcaaa cagccccaac
     8101 gccagcccca cctgggtatc aaattcccgt tcctggttaa cagcggcctg ctcaagcatg
     8161 gtttccagtt cgcctctctg ttcgccggaa gcgataatgt acagcatcat cggcgggaac
     8221 aggcgcaact ccgccagcgc ggcgcgcaga ctgcttcctt cgcggacgcg atctgccgcc
     8281 agcagcagtt gttgttcgac atagcgattt gccgacacgg cggcagcggt ctgaatgcct
     8341 tccagtagcg gaacactgct ggcggtgagg atgcttaatg tgcgggaaaa acgcgccgta
     8401 ttcagcccgc gcgcaacacg gcccgtcacg ggcagacgca gcaaggtttt atcccagcgc
     8461 aggcgcatag taggattttt aagtagccat tgccccagca ccagaagcgc cagcaatccc
     8521 gccagccagt aaacgccgct ggcctgtaac gcatcactca tagcgataag cgcacgggtg
     8581 gaggagggta gcgcgtgtcc gagatgatca aactgttcga tgattttcgg caccactgcc
     8641 gtcagcaaaa tagtgaccac gcccgttgcc accaccagca taaccagcgg atagagcatg
     8701 gcctgcaata gacgtgattt caggcgctgt cgctgttcgg tgtaatctgc caggcgattg
     8761 agcaccacgt cgagatgccc ggatttttct ccggcagcga ccatcgaaca aaacagggaa
     8821 tcaaagacgc ggggatgttc gcgcaggctg tccgacaggg tataaccttc ctgaatccgg
     8881 ctgcgcagcg ccattccgag gctcttcaca tgcagttttt cactttgctc actgaccgcc
     8941 tgcaagcagg tttccaacgg cattgctgcc tgcaccagcg ttgccagttg gcgcgtgaac
     9001 agcgccagat ctgccgccgc cacgcgacga tgtgcgtgcc gccgacgctg caacattccc
     9061 cctgccgatg cattcatcct ggcttcaatg tgcacgggga taagctcttt accgcgcaac
     9121 aactggcggg catgacgcgc ggaatccgcc tcaatcatgc ctttggtttt gcgaccatta
     9181 cgctccagcg cctgatagta aaacagtgcc attacgcctc catggttacc cgcagaactt
     9241 catcgagaga ggtttctccg gcgagcactt tctcaatgcc gttgctgcgg atacccgcag
     9301 agtgttgccg gacataacgt tccagctcca gctccccggc ctgacggtgg atcaaatcac
     9361 gcaatgtggc atccaccacg tcagctcatg gatggcagtc cgtccgcgga aacctttgtg
     9421 attacaggcg gggcagccct gcggatggta cagagtgacg gttcgcgcat cggtaatgcc
     9481 cagcaggcgt ttttcttcgt cagtggcagg cgcggcctga cggcagtcgg gacacagcgt
     9541 gcggaccagt cgctgcgcca tcacgcccgt cagactggaa gagagcagga aaggctccac
     9601 gcccatatcc tgcaaacgcg tgatcgcccc caccgccgtg ttggtatgca gcgtggaaag
     9661 gaccaggtgt ccggtaagcg atgcctgaac agcgatttct gcggtttcgg tatcgcggat
     9721 ttcaccgacc atcaccacat ccgggtcctg acgcaaaatc gcgcgcaggc cacgggcgaa
     9781 ggtcatgccg acgcgggtgt taacctgtgt ctgaccgatc ccttcaatca tgtattcgat
     9841 ggggtcttca accgtgagaa tgttacgcga atggttgttc agctcctgca atccggcgta
     9901 cagcgtggtg cttttgccgg aacccgtcgg ccccgtcacc agaaagatgc cgtgcggttt
     9961 gtgtaacagc tggcgcaact gcacggtcag ttgctggcta agccccagac gctccagcgt
    10021 caggcgggcc tggtttttgt ccagcagtcg cagcaccacg cgctcgcccc aggcagaagg
    10081 catggtggag acacgcacgt caatcgcccg gccgcccagc agcagcgcaa tacgtccatc
    10141 ctgcggtacg cgcttttcgg cgatatccag ccgtgccatc accttgatac gcgataccag
    10201 cagcgaggcc agtttgcgcc ccgggcgcag catttcatgt aatgtgccgt caacgcgaaa
    10261 acggatcacc agactctttt caaacgtctc gatgtggata tccgaagcgc cttctttaat
    10321 cgcctctgcc agcatggcgt tgatcagttt gatgatcggc gcatcgtcgt cactttccag
    10381 cagatcttcc gtttctggca gttcttccgc gagagtaaaa aagtcctcag cagaaccgag
    10441 atcttccatc agctggcgag cctcggaaga gtcccgctgc cagaccgcat tcagccgctg
    10501 ttcaaattcg gcctcgtcga tttgccgcag cgtaaagggc gcgttcagcc cccgctgcag
    10561 ctcctgcaaa acagagagcg atagcggatg aacgtggagg atctccagcg acgcttcgca
    10621 ccatgccacc aggctaaacc gacggctgaa actgtagggc agacgcacgg tcttggcggt
    10681 ggtttcctgt gctacaggca ccattaacgc gttctcccgg cattgaggaa cgcgcgaact
    10741 tccggcggta aggcctggtt ttgcgctggc agtaccggct gtgcggtgtg cggcatcagg
    10801 cttaaacctt gctcatcacg atagatctgt tcggcgcgca tatagttata tttgcgctgc
    10861 gacacgccgt ctgccgccat accgtcatgc agaatggtcg ggcggataaa caccatcagg
    10921 ttacgttttt cttttttatc cgccgtcgat ttaaacaggt taccaatcaa tgggatatcg
    10981 cccagcagag gcactttcgc cacgctttct cccgcctgat cgtccatcag accaccaagc
    11041 acaatcagct caccatcgtt agccagcacg gtggttttca gtttgcgctc accaaacacc
    11101 acgtcgaggc tggtctgtcc ttccaccttc gagacttcct gctcaatcac catctgtacc
    11161 gcgttacctt cgttaatctg cggcgtgact ttcagcatga tgccgacttt tttcctttct
    11221 actgtattga aaggattgct gttattggag ccaacggtag agccggttaa taccggaacg
    11281 tcctagccca ccatgaagaa ggcttcctgg ttgtccagcg tagtgatgct cggcgtggag
    11341 agaacgttcg agctggagtc gtttttaacc gcctgcacca gtgccatcca gtcgcctttc
    11401 accacgccaa ccgccgtacc gctaaatcca gaaagaagct gggcgagtgt ggagagatcg
    11461 ccgttggtat ccggattaat ggtggtagcg ccgttttcac tgatcaccgt cgagcctttc
    11521 tgcggtttcg cctgagaaat ggctgccccc agcgtaccga tagggatctg cgtaccatta
    11581 gcaaactgca ttaatccggc atctttcgat gcccactgca cgccgaagtt gatattgcta
    11641 ccttcggcaa cttccacgat caacgcctcg acgtgcacct gagcacggcg aatatccagt
    11701 tgttcaatta cgctttgcag cgactgcatg atgtcctgcg gcgcggttac aatcagggca
    11761 ttactgtgtt tgctggcggc gatggataca acctcacgcc cgctaccgac cgtgccttcc
    11821 gcctcttctt tagccgccgt gagcgtgccg ctgacctgtt tcagcacatc gaccagatct
    11881 tcggctttgc tgtatttgag atagaaaacc tggctgttgc cgctgcgttc catttctgag
    11941 tccagccgac ggatcaggcg gcgcattttg tcccgcgtgg ccgggtcacc actgacaatc
    12001 acactgttgg tgcgttcgtc ggcgacaatt tgagatttca gcgtcgcagg ctggttctcg
    12061 ccgctgtttt tcgtcaggct ttccagcacg cgggcgattt ccgaagccga ggcgttatcc
    12121 agcgggatca cctcttcggt gcgattaccc gcgtgatcca cgcgctggat cacttccgtc
    12181 aggcgctcca caacggaggc gcgtccggtg agcataatca cgttggaggg atcgtaatta
    12241 acaacgttgc ctgagcctgc gctgtcgatc atctggcgca gaatcggtgc cagctcgcgt
    12301 accgacacat tacgtaccgg cactactttg gtgaccattt catcgcccgc gtagttgtcg
    12361 ctgccttcac cgaccagcgg cagcggctcg actttcgcag cgctggattt caccaccttc
    12421 agcacgtcgt tttccatcgg tacgacggca tacccctgtg cttccagcag gttaaggaat
    12481 agctggtaat actggcgttc attgagcggg gtcatggtgc gaatactcac tttcccctgt
    12541 acgcccggcc ccataatgat ggttttatta aggttagcgc cgacggtttc gatgaacgat
    12601 ttcaggtcgg tatctttaaa attagcggtg aaagtggctt cttccgccca gaccggtgaa
    12661 ctgcataatg ccgctgccag caccagcggc agtaaacgct tttttgtttt gaggccagtt
    12721 gtcttcttac gccagaccga caaggtaata tcacgccaaa acacgatgaa tgattctcct
    12781 ggattaaatg cggttagcgc agcgcgatgg aaatgtcgtg gcgcgcaccc ttgcgtaaaa
    12841 ccgtaagttg aatggaatcc attgaaggta actgccgcat cagagcaatc attgctcgtg
    12901 gatcagtgaa atcctgctga tttagcgcaa tggcgatatc gccttccttg aaaccgctgg
    12961 catcgaacag agaacgatct gcccccggct tcactgcata accgacaatt ccctccttac
    13021 gcacaggcgt aagctggata tagttaaaaa ttttctgcgg atctttcgcc agtgcctgac
    13081 gcacggcagt cggtatctca actggcgcac tgacagcagg ttcagcaaca gcttgctttg
    13141 cttcgtcact gacagctttt ttgttggtcg cggcaacggt ggaacgctcc tcttcagcca
    13201 ggctcaggcg ctctattttt ccctgatagc gcagcatcac atggtcgcga ttgatttcct
    13261 caatcaccgc gttgtgcgag ccaagcgttt caccctgcaa atagacctgc tgtttaccgc
    13321 cttcttcaat aaccgcgccg ggtctggcac caaaggcgat cccgcgcgca ccacattaag
//
LOCUS       DAJVOY010000105        13366 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVOY010000105 DAJVOY010000000
VERSION     DAJVOY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.105"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OQL90_002693"
     CDS             complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OQL90_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCS1369416.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYLNFSLEHA"
     gene            1197..2282
                     /gene="dmlA"
                     /locus_tag="OQL90_002694"
     CDS             1197..2282
                     /gene="dmlA"
                     /locus_tag="OQL90_002694"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCS1369417.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2532..4142
                     /gene="yeaV"
                     /locus_tag="OQL90_002695"
     CDS             2532..4142
                     /gene="yeaV"
                     /locus_tag="OQL90_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCS1369418.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4174..5298
                     /gene="yeaW"
                     /locus_tag="OQL90_002696"
     CDS             4174..5298
                     /gene="yeaW"
                     /locus_tag="OQL90_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCS1369419.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5354..6319
                     /gene="yeaX"
                     /locus_tag="OQL90_002697"
     CDS             5354..6319
                     /gene="yeaX"
                     /locus_tag="OQL90_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1369420.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OQL90_002698"
     CDS             complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OQL90_002698"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1369421.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OQL90_002699"
     CDS             complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OQL90_002699"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1369422.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OQL90_002700"
     CDS             complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OQL90_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1369423.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OQL90_002701"
     CDS             complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OQL90_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1369424.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10833..12743)
                     /locus_tag="OQL90_002702"
     CDS             complement(10833..12743)
                     /locus_tag="OQL90_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1369425.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            12875..13219
                     /locus_tag="OQL90_002703"
     CDS             12875..13219
                     /locus_tag="OQL90_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1369426.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3199 a         3554 c         3334 g         3279 t
ORIGIN      
        1 accagatagg tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt
       61 atctttcgat catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa
      121 ggagaaaatc aggttttaac ccgatatcaa cccgataatt gaatcattaa tcaggcatgc
      181 tccagtgaaa aattcaggta gtgctcggca aaatactggc gtaaaaattc taccgttatc
      241 cgcactttcg ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg
      301 taatattccg gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac
      361 caggaacgca gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga
      421 ttagacgaca gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc
      481 cagacaccaa aaggatgatc gcgctcttta atgaccaggc acggcaatgc agaaagatcg
      541 gttaaatgtt tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg
      601 taattggtcg ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata
      661 tcgagatcga caccttcatt gaccaaatcg accagccggt cttccacatc aaaacgtaat
      721 tccagttgcg gatacgcctt cgccagcgct gataacgccg gagccaccac ctgtcgtcca
      781 aaaccaaagc tgctgataat acgcagcatc ccctgtggca cctgacgcac gtcagaaagt
      841 tcgtccatca tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct
      901 ccttcttcgg taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt
      961 tgctccagca aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc
     1021 acggcggcaa aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc
     1081 ggtaaattat tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc
     1141 cgtcaattat aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga
     1201 tgaaaacgat gcgtattgct gcgatcccgg gagacgggat tggtaaagaa gtccttcctg
     1261 aagggattcg cgtgttacag gctgccgctg agcgctgggg cttcgacttg agttttgagc
     1321 aaatggggtg ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact
     1381 ggcatgagca acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggata
     1441 ccgttccgga ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc
     1501 agtacatcaa cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa
     1561 aacagcctgg cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct
     1621 cgctcggcgg tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat
     1681 ttacacgtcg tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc
     1741 cacgtaagac gctaacttat gccactaaat cgaacggttt agccatcagc atgccgtact
     1801 gggatgagcg agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc
     1861 atattgatat tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg
     1921 cgtccaattt gtttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg
     1981 gcattgcccc atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagcctg
     2041 tccacggttc cgcgccggat atctacggga aaaatattgc taaccctatc gccacgattt
     2101 gggccggggc aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc
     2161 ataacggtat tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata
     2221 tgaaaggcaa tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt
     2281 aaggtcaaac cagtttattt gaaccgcgtc actgacgcgg ttttttattc gttctttgca
     2341 gtaaataacc tgcgtcattt caccttttat tgtttccgtt tcgtgtttta tggctttccg
     2401 cattcttaat tgtttaattt atgtaatatt caaataatca atttgttaaa cacaacatac
     2461 aactcattac cctgcaaata attacaactg gcaaaagcca gagacgggct tttatgccca
     2521 ggagatgact catgatgagc aatgttaaga aaaaagatgt gccgctgata agcatcagcc
     2581 tggtggccat tcttttcatc gcagctgcat taagcctttt cccacaacaa tcggccgacg
     2641 cggccaacgc aatatacact tttgttacgc gtacgttagg ttccgccgta caggtattgg
     2701 ttttgctggc aatgggactg gtgatttatt tagccaccag taaatacggc aatattcgtc
     2761 ttggcgaagg aaaaccggaa tacagcacgc tctcctggct gtttatgttt atttgtgccg
     2821 gtttaggttc ttctacgctt tattgggggg ttgctgaatg ggcctattat tatcagacac
     2881 ctggattaaa tatcgcaccg cgttcacaac aggcactcga atttagcgtt ccctactctt
     2941 tcttccactg gggcatcagc gcctgggcaa cttatacgct ggcctcatta atcatggctt
     3001 atcactttca tgtgcggaaa aacaaaggtc tgagcctttc cggcattatt gctgctatta
     3061 ccggcgttcg cccgcaaggc ccatggggaa aactggttga tttgatgttc ctgatcgcca
     3121 ctgtcggcgc actgactatt tcccttgttg tcaccgcagc aacctttact cgtggacttt
     3181 ccgcactgac aggtttaccc gacaacttca ccgtgcaggc atttgtgatc ctgctttctg
     3241 gcggcatttt ttgcctaagc tcgtggattg gtatcaacaa cggtttgcaa cgtctgagca
     3301 aaatggttgg ctggggcgcg ttcctgctgc cattactggt gctgattgtc ggcccaaccg
     3361 aatttattac caacagcatc atcaatgcca tcggcctgac cacgcaaaac ttcctgcaaa
     3421 tgagcttatt caccgatccg cttggcgatg gttcatttac ccgcaactgg accgttttct
     3481 actggctgtg gtggatctca tacacccctg gcgtagcaat gtttgtcacc cgcgtttccc
     3541 gcggtcgtaa gattaaagaa gttatctggg gactgatcct cggcagcacc gtcggttgct
     3601 ggttcttctt cggcgtaatg gaaagctatg ccattcatca gtttatcaat ggcgtaatca
     3661 acgtcccaca ggtgctggaa acactgggcg gcgaaacagc tgtacagcaa gttctgatgt
     3721 cgttgccagc cggtaaattg ttcctcgccg catacctggg cgtgatgatt attttccttg
     3781 cctcgcatat ggatgcagtg gcctacacca tggcggcgac cagtacgcgt aatctccagg
     3841 aaggtgacga tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc actctgatcc
     3901 cgctttccat cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc gtcgtgctca
     3961 cagcccttcc cttcctcgtc attttactgg tgaaagtcgg cggatttatt cgctggctga
     4021 aacaggatta cgccgacatt ccggctcatc aagttgaaca ttatctcccg cagacaccgg
     4081 ttgaagccct ggaaaaaaca ccagtgatcc ctgcgggaac cgtattcaaa ggcgacaact
     4141 gagcgccatc atcctaacga taaaggtatc cctatgagca atctgagccc tgactttgta
     4201 ctacccgaaa atttttgcgc taacccgcaa gaggcgtgga ccattcctgc ccgtttttat
     4261 accgatcaga acgcgtttga acacgaaaaa gagaacgtct tcgccaaaag ctggatttgc
     4321 gtcgctcaca gcagcgaact ggcgaatgcc aatgattatg tgacgcgtga gatcattggc
     4381 gaaagcatcg tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta taacgtgtgt
     4441 ccgcaccgtg gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt gattacctgc
     4501 ccgtatcacg catgggcatt caaactcgat ggcaatctgg cccatgcacg taactgcgaa
     4561 aacgtcgcca atttcgatag cgacaaagcg caactggttc cggtgcgtct ggaagaatat
     4621 gccggattcg tcttcatcaa catggacccc aacgccacca gcgtagaaga tcagttaccc
     4681 ggtctgggcg cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa actggcggcc
     4741 cgctttacca cccgcacgtc tgccaactgg aagaacattg tcgataacta tctcgagtgc
     4801 tatcactgtg gtccggcgca tccaggtttc tccgactccg tacaggttga tcgttactgg
     4861 cacaccatgc acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc cgaacagtcg
     4921 tttaaatttg aagagggtac ggatgcggca ttccacggtt tctggctgtg gccgtgcacg
     4981 atgctgaacg tcaccccgat caaagggatg atgacggtca tttatgaatt cccggtggat
     5041 tctgaaacta ccctgcaaaa ctacgatatt tacttcacca atgaagagtt aaccgacgag
     5101 caaaaatcgc tgattgagtg gtatcgcgat gtgttccgtc cggaagattt acgtctggtt
     5161 gaaagcgtac agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg catcatggcc
     5221 gacagtagcg gtagtggcat ttccgaacat ggtatcgccc atttccataa tctgctggcg
     5281 caggtgttta aggactaatg acatcggcgg cggtattttc cgctgctggg ctgatttttg
     5341 atggagtaca gcaatatcag actatcaaat gtttgaagta caggtgagcc aggttgaacc
     5401 ccttaccgaa caggtgaaac gcttcacgct ggtggcaacc gatggcaaac cgctgcctgc
     5461 gttcaccgga ggaagtcacg tcattgtgca gatgagcgat ggtgataacc agtacagcaa
     5521 tgcgtattca ctactgagtt cgccgcatga cacctcttgt tatcagattg ccgttcggct
     5581 ggaggaaaac tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag tgggcaatcg
     5641 gttaacgatt tcaacgccta ataacctgtt tgcgctaatt ccctcagcca gaaagcatct
     5701 gtttatcgcg ggcggtattg gtatcacccc tttcctgtcg cacatggcag agctgcaaca
     5761 cagcgacgtc gactggcagc tacattactg ctcgcgaaat ccagaaagtt gcgcatttcg
     5821 tgatgagcta gtccagcatc cgcaggctga gaaagttcac ttgcatcact catcaaccgg
     5881 aacacgactg gaattagcgc gattattggc ggatatcgaa cctggcacac acgtttatac
     5941 ctgtggtccc gaggcgctaa atgaagcggt aagaagtgaa gctgcgcgtc tggacatcgc
     6001 cgccgatacg ctgcactttg agcaatttgc tatcgaagac aaaaccggcg atgcctttac
     6061 cctggtgctt gcccgttccg gaaaagagtt tgtggtgccg gaagagatga ctattttgca
     6121 ggttattgaa aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag gggtatgcgg
     6181 aacctgcgaa acagcaatac tggaaggtga agctgaccat cgggatcagt attttagcga
     6241 tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga agggtaaacg
     6301 cctggtgttg gatttgtagt ttgccaaagc cggatgtggc gctgagcgcg cccagtccgg
     6361 cttcggaaga ttttactgcg gatattcctg caataaattg tgtaatgctt ccgccatcag
     6421 ctcaccacgc cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg
     6481 ccagttcagc agttggttga tttgccgacg cgatgccagc aattcggcgc tgatcttatg
     6541 cgtttcgctc acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataaccagg
     6601 catgtccatc aggttaagca tcggctgcgg taaggcctct tccggcaatg tctgcgcttt
     6661 ttccaccagc gtcagcagcg ttttaccgtg aaagcggatt tcgctaccgg ataaacccag
     6721 gctgtccagt tcgcctaaac ttcccggcat ataacgcgcg accgaccaca aatgctcttc
     6781 acgcacgaca aagttcaccg ccagatcacg ctctcgcgcc ttgcgcagtc gccagtcggc
     6841 taacagttgc agacaggcca gttggcgtgt gcgtaactgc caggcattgg tgatatcacg
     6901 ccaggcatct tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc
     6961 cagcgccgca ggtagccagc cggaggcctc cgtttctacc ataagcttgg cggtgatcgg
     7021 taacagatac cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc
     7081 cagccagtcg gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat
     7141 ggaagcgaaa ccccatgaca tcgggcgtcc gcagaaggca gaaaggattt gcgtgtcaat
     7201 caagggttgt ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc
     7261 atggagaaat tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt
     7321 gatcccgaga ggatcaatta gcgccagatg ctcgccatcg aaaagttgaa tcaaccccag
     7381 ctgcgggtaa taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc
     7441 acggacggct tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa
     7501 attgttttct ctttagtggg cgtcaaaaaa acgccggatt aaccggcgtc tgacgactga
     7561 cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc
     7621 aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact
     7681 ttgtatcccg tgagctgacg gcggcaaaaa gtcaccagtg actcttcggt aagcgatgga
     7741 tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca
     7801 gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag
     7861 acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaatcct
     7921 tcttcatcca ttaccgcgat gtcgccggtg tgtaaccagc catttttgat gatttcatcg
     7981 gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca
     8041 cccggttgac ctggtggtac ttcattatca tcatcatcca ccagtttggc ttccgtcgac
     8101 ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc
     8161 agcggcgcac actcggtaag gccatagcct tccagcagat actgtccggt cagtttcacc
     8221 caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg
     8281 gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg
     8341 ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcacgc
     8401 gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc
     8461 agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc
     8521 aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc
     8581 atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc
     8641 ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat
     8701 gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa
     8761 ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc
     8821 tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca
     8881 accgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg
     8941 ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc
     9001 aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccac cccttgttgc
     9061 aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat cacctccccc
     9121 atattcacaa acgcaggctg atcggcgtag cgcgcgaccg actgctcaaa catatctacc
     9181 agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata acggttaagc
     9241 caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat
     9301 gatatacatg ccgttaacat aatattaact catcatacca gcttgataat tacccaacga
     9361 aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg cagaaacagc
     9421 ggaccagccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac aactgtttgt
     9481 actctcgcgg gaccgggatt ataccagccc catccgccgt agccataggg ccagccacga
     9541 ccgccataaa accatggatc aatcggctga ggcggcataa tcacctgctg ggttaaatgc
     9601 caacgtttgt aacccgttac ttgcatcacc ataaatttat agggcgtatt gccgattttg
     9661 ccgtcaaccg caccagtgat tggcccgact accgtaacca gttgtccacg gaaatccacc
     9721 gggtccagaa aaccgttcac atcggcataa atgcgaccgc gagaaggttc tcccagcgtc
     9781 ggtctggctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg
     9841 ttttgtaccg caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc
     9901 gcactcatca cccgaactaa atcttgttgc ggcgtgggac tgcggccttt aatggcgtcc
     9961 ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata
    10021 acattctttt gaaccgccat gatgcgactc ctttttttct cagagcatac tcttaagatt
    10081 cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca
    10141 tgttccaccg ccaccgtttt accctcggca aatatctgac acgcaatcgg cagcatatct
    10201 tccgtcgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttaccgaga
    10261 tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt
    10321 cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg
    10381 ttttcatcac gctgatattc ggcccagtaa acttcgccca tccgcgcatc aatggcagcc
    10441 agcacgcggg ttgcgccgtt tttgcgccac gccccttgcg ccattgtcat tagcgtggag
    10501 acgccaatca tcggcagctc tgcgccaagt gccagccctt gcgcaatgcc aataccaatg
    10561 cgcacgccgg taaagctacc ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg
    10621 gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca
    10681 cgagggcaaa gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc gacagagcag
    10741 gcctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa
    10801 aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa
    10861 cgaaccgcac gggcaatgtc acgggtgcgc ggcgcgggcg gcagactggc gagaaacgtc
    10921 gcgccgtaag ggcgcatcac cagccgattg tcacaaatca ccaaaacccc gcgatcgtcg
    10981 gcgtcgcgaa tcagtcgccc taccccctgc ttgagagtaa tcaccgcatc cggcagttgt
    11041 acttcatcga acgggtcgcc accgcgcaaa cggcaatctt ccatgcgcgc ttttaacagc
    11101 gggtcatccg gtgaggtaaa cggcaacttg tcgataatca ccaatgacaa tgtatcgcca
    11161 cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg
    11221 acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc
    11281 atggtagcgc ggaactgctc ggccagatca cgcatcatgg cgtgcgaggt acaaagcata
    11341 aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca
    11401 gaacctggct ggttggtttg cggcagattg cgcggcacac agagtaacgc ctggcggctg
    11461 taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg
    11521 aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccaggc
    11581 ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta
    11641 aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc
    11701 cgcttcagcc gtgtgcgata caacgtagcg cgctcaaatg ccgcatccag caaggcggaa
    11761 cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa
    11821 aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc
    11881 tcaccgagtt gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc gcacttttgt
    11941 aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg
    12001 agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg
    12061 tgggcttcgt cgaagatcat gacgtccgct tccgggatca gttcgccaaa tccactctct
    12121 ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc
    12181 gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga
    12241 cagttgtcgt tggtgctggt aaccagcggc cacgcctgtg aatcttccgc cacgctgacg
    12301 caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg
    12361 cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg
    12421 cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt
    12481 gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag
    12541 ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta
    12601 ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc
    12661 gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg
    12721 tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacgggga ttatgtcagg
    12781 atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc
    12841 agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga
    12901 agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga
    12961 aaacctcgac gccgatgctt ttgagcaaac cgccaacacg ctggcgcaga ttgacgccgt
    13021 gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt tcctggctga
    13081 taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtgg cgggtcatgc
    13141 gccggtgcgc tgcacggtgc aagcggggct gatgaacccg aagtataaag ttgaaattaa
    13201 gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata atttatcgat
    13261 aatactggtc ggttttacat aaatcgacca gagattaaga tattactgtt gatgctcctg
    13321 ctaattcatt atttattttt ataatttcaa ttttagctat aaagct
//
LOCUS       DAJVOY010000106        13312 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVOY010000106 DAJVOY010000000
VERSION     DAJVOY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.106"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..308
                     /locus_tag="OQL90_002704"
     CDS             <1..308
                     /locus_tag="OQL90_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1369427.1"
                     /translation="VLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="OQL90_002705"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="OQL90_002705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1369428.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OQL90_002706"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OQL90_002706"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1369429.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3390
                     /gene="ilvY"
                     /locus_tag="OQL90_002707"
     CDS             2500..3390
                     /gene="ilvY"
                     /locus_tag="OQL90_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1369430.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3387..4931)
                     /gene="ilvA"
                     /locus_tag="OQL90_002708"
     CDS             complement(3387..4931)
                     /gene="ilvA"
                     /locus_tag="OQL90_002708"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1369431.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4934..6784)
                     /gene="ilvD"
                     /locus_tag="OQL90_002709"
     CDS             complement(4934..6784)
                     /gene="ilvD"
                     /locus_tag="OQL90_002709"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1369432.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6849..7778)
                     /gene="ilvE"
                     /locus_tag="OQL90_002710"
     CDS             complement(6849..7778)
                     /gene="ilvE"
                     /locus_tag="OQL90_002710"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1369433.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7798..8061)
                     /gene="ilvM"
                     /locus_tag="OQL90_002711"
     CDS             complement(7798..8061)
                     /gene="ilvM"
                     /locus_tag="OQL90_002711"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1369434.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8058..9704)
                     /locus_tag="OQL90_002712"
     CDS             complement(8058..9704)
                     /locus_tag="OQL90_002712"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1369435.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9707..9757)
                     /locus_tag="OQL90_002713"
     CDS             complement(9707..9757)
                     /locus_tag="OQL90_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369436.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9844..9942)
                     /gene="ilvL"
                     /locus_tag="OQL90_002714"
     CDS             complement(9844..9942)
                     /gene="ilvL"
                     /locus_tag="OQL90_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1369437.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10294..11814
                     /locus_tag="OQL90_002715"
                     /pseudo
     CDS             10294..11814
                     /locus_tag="OQL90_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11839..12177)
                     /gene="maoP"
                     /locus_tag="OQL90_002716"
     CDS             complement(11839..12177)
                     /gene="maoP"
                     /locus_tag="OQL90_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1369438.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12296..13135
                     /gene="hdfR"
                     /locus_tag="OQL90_002717"
     CDS             12296..13135
                     /gene="hdfR"
                     /locus_tag="OQL90_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1369439.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13231..13306)
                     /locus_tag="OQL90_002718"
     tRNA            complement(13231..13306)
                     /locus_tag="OQL90_002718"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13271..13273),aa:Trp,seq:cca)
BASE COUNT         3093 a         3600 c         3447 g         3172 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 gcctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctggta caacaagtca
      181 gtcgaaaccg attaaataga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atattgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactgaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt tcgataaagt tgtgttctct tgtccggtac
      721 tggagccgac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcaa cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactca gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gctcgtgcag tgattcataa tatgcagact
     1201 cttcaatgat gccggaatcg accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat aagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gcgagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgcac ttcggtgcca gggcatttcg gcgcaaccat gactacggtg atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta cgtcggagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccaa atccgcctgc gggatcagtt cttcgtaagt gcccacttta aaaccgtttt
     2101 cggtcgcttt acgccaggat gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacattgagg gctgctccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtgacgctg actgaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcag ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgcca
     2821 ccgattctcg accgcttccg cgcggaacac ccgtcggtgg agatcaaact aaccactggt
     2881 gatgcggcag atgcgatgga aaaggtagtc actggcgaag cggatctggc gattgccggg
     2941 aaaccggaga ctctgcccgg cgcagtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgcgc ccgcgctgcc ctgcccggtg cgtaaccagg tttcgtcaga gaagccggac
     3061 tggtctacag tgccgtttat catggccgat cagggaccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggcgtggcgc tgttgccgga agtagtgctg
     3241 gaaaacagcc ccgaaccggt gcgtaaccgc gtgatgattt tagagcgcag cgatgaaaaa
     3301 acgccgtttg aactgggcgt ttgtgcgcag aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga agattttgcc gaaccattaa cccgccaaaa agaacctgaa cgccggatta
     3421 ttggtttcgt cgtggcaatc gtagcccagc tcatttagcc gggtttcgaa atccggttca
     3481 tggtcgccga gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag
     3541 tggaacaaag aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc
     3601 ggtgattccg ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg
     3661 ccgaccatat agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg
     3721 tagccgccgt cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg
     3781 cgcacgccga caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg
     3841 accgctcgcc cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg
     3901 gtcaccgcca acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc
     3961 aggccgtgga agttcacgtt ggcaccggaa agaatatgcg ccagccgttc gccgcgaatg
     4021 ttgtgctggg cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc
     4081 accgcgcgca catcttcgaa taaatccttc atcgccgcac agatcgcatc actatcgacg
     4141 gtgatgatgt cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt
     4201 accgctacgc cttcagcaaa tagccctacg cgcggcagat caaccggatg acccgcatcc
     4261 agcgctgctt tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgtggc
     4321 atcagttgtt tgatcagcac cgcaacgccc gccgccagac cgccgccgcc gactggcaca
     4381 aatacgcggt cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg
     4441 gcaatcacca tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt
     4501 tcgatcgctt tagctttcgc ttcgtcaaag ttcgcgccat gaagcagcac ttcgccgccg
     4561 aatccacgta ccgcatcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc
     4621 ttcacgccta accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca
     4681 gtgatcacgc cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg
     4741 cgcagcttaa agctgtgcac tggctggcga tcttcgcgct tcaccagaat gacgttatca
     4801 agacgcgacg acagtttttc cattttttgt agcggcgtaa cctgcgctgc ctcgtaaacc
     4861 ggcgcgcgca gcactgctct taaatattcg gcaccttccg gagcaccaga caggggttgc
     4921 gagtcagcca ttattaaccc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc
     4981 tggttgccag gctggcgtag gcgcgcaggg caaaggaaac ctgacgttca cggtttttcg
     5041 gcgtccaggc tttgtcaccc cgagcttcct gcgcttcacg acgcgccgcc agttcggcat
     5101 cgcttacctg taactgaatg ccacggttcg ggatgtcgat agcgatcagg tcaccatctt
     5161 caatcagacc aatgctgccg ccgcttgccg cttccggtga gacgtggccg atggaaagac
     5221 cagaggtgcc gccagagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca
     5281 ttgatttcag gaagctggtt gggtagagca tttcctgcat gccagggccg cctttcgggc
     5341 cttcatagcg gatcaccacc acatcgcccg cgacaacttt gccaccgagg atcgcttcta
     5401 ccgcgtcgtc ctggctttcg tacacttttg ccgggccggt gaatttgagg atgctgtcat
     5461 cgacgcccgc ggttttcacg atgcagccgt tttcagcaaa attaccgtag agcaccgcca
     5521 ggccgccgtc tttgctgtag gcgtgttcca gcgagcggat acagccgttt gcgcgatcgt
     5581 cgtccagcgt atcccaacgg caatcttgcg agaatgcctg ggtggtacgg atacccgcag
     5641 ggccggcgcg gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgct
     5701 ccagcgtttg cggcaacgtc aggccgagca cgtttttcac atcacggttc agtaaccccg
     5761 cgcgatccag ttcgccgaga ataccgataa caccaccagc acggtgaaca tcttccatat
     5821 ggtatttctg ggtgctcggc gcaactttac acagctgtgg aaccttgcgg gaaagcttat
     5881 cgatatcact catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagta
     5941 cggtgttagt cgatccaccc atcgcgatat ccagcgtcat ggcgttttca aacgccgcct
     6001 tactggcgat attacgcggc agtgcacttt cgtcgttttg ctcgtaataa cgtttggtca
     6061 attcaacaat gcgtttacca gcattaagga acagctgctt acggtccgcg tgggttgcca
     6121 gcagcgagcc gttgcctggc tgcgacaaac ccagcgcttc ggtcaggcag ttcattgagt
     6181 tagcggtaaa catcccggag caggaaccgc aggttgggca ggcggaacgt tcaacctgat
     6241 cgctctggga gtcagaaact ttcgggtctg cgccctggat catcgcatca accagatcga
     6301 gtttgattat ctgatcggac agtttggttt tcccggcctc catcgggccg ccggaaacaa
     6361 agatcaccgg aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc
     6421 agttagagat gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat
     6481 cagcgatcag ttcgcgagat ggaagtgaat aaagcatccc cccgtggccc atggcaatcc
     6541 catcatccac cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt
     6601 cggcgaccag tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg
     6661 agttcacaac cgcgataatc ggcttgccaa aatcggcgtc ggtcattccg gtggcgcgcc
     6721 acagcgcacg agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag
     6781 gcatacttta tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt
     6841 ttttgtattt attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccagtg
     6901 aagaggccga agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca
     6961 acctgaatac cgtctacgct gcgcactggc gtgatttctg ccgcagtacc ggacataaac
     7021 acttcatccg ccaggtacag ggattcgcgc gacagcacct gctcacgcac ttcaattccc
     7081 agctctttcg ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggatgaggta
     7141 aacggtgggg tgaacagcac gccgtctttc acttcaaaca ggttttcacc tgcgccttca
     7201 gagatgtaac cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg
     7261 ctacccacca gcagggaaga gaggtagtta ccaccggctt ttgccgccgt cgggatggtg
     7321 tttggtgctg cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct
     7381 gcgcccagat acgctcccca cgggaaagca gcgataatca cgtcggttga gtatcccgct
     7441 ggcgggttta ctcccatgcc aacatcaccg acgaagatca gcggacggat ataggcgctg
     7501 gtgagattat ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgc
     7561 gaaaccggga agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg
     7621 cggaatacaa ccggtccttt gtgcgagtcg tagcaacgga tgccttcaaa aaccgaagtg
     7681 ccatagtgca gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc
     7741 ccattgaacc aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca
     7801 ggcgcggatt tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac
     7861 cagtttattt aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc
     7921 gatatttata ttttgtgcat cgctggcggc ggccatattc attgagcaga cgtggaaacc
     7981 acgatgacgc accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac
     8041 attgacctga tattgcatca tgataatttc tccaacattt ctgaattact ggcgccaggc
     8101 ggcaccagcg gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca
     8161 tcactgttca gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg
     8221 ccagggatgc cgaaggcgct ggctaacatg aggaaatcgg ggttatcagt aagggtggtt
     8281 tcgctgtatc gttcctgaaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg
     8341 ttatcgagta agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc
     8401 acattcatca tgaaagagcc gtcaccggag atacagacga cggtatcgtt cggtcgcgcg
     8461 acttgtgcgc caaccgccgc tggtaaacca aaacccatag tgcctaagcc gctggaggtg
     8521 atgaaatttt ccgggcgagt gtgggcgatg tgttgcgcag cccacatctg gtgctgcccc
     8581 acatctgtgg tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac
     8641 ggcgcgtaga tagcgtcgcc gggatggtcg taacgccagg catgttcatc acgcagctgc
     8701 gcgcagtatt gctgccagtc attgatattt aacggctgct gtaattctgg taacagagca
     8761 tttaaatcac cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg
     8821 atatccatat ggataacgct ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg
     8881 tcatcaaaac gtgcacccac ggcgatcagc aagtcgcact cctgcaccgc gaagttcgcc
     8941 gctttggtgc catgcattcc cagcatgccc agatagtacg gataatctgc ttcaactgcg
     9001 cccagccctt tcagcgtgca ggtggcaggc atttttgtgg tagcgagaaa ttctcgtaaa
     9061 gcaggaactg cctgcgccat acccacgcca ccaccaacgt acagcatcgg tttttgcgct
     9121 tttgccagca tctggcgcgc ttgctcaact tcggcatgtg ggaaagtcac ttcgttttca
     9181 acggtggtga accacggttc caggtcaccg ctggctagct ggatatcttt tgggatatcg
     9241 accagaaccg gaccaggacg acctgagctg gcaacgtcga atgcttcagc cataatgcgc
     9301 ggcaactctt ccagcgactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc
     9361 aggacatcca cttcctgaaa tgcatcagtg ccgataaacg gtgcggacac ttgaccggtg
     9421 atggcaacaa cagggataga atctaacagt gcgtccgcaa gcccggttat caggttggtt
     9481 gcgcccggac cagacgtggc gatacatacg ccagttttgc cggtagcacg ggcataaccg
     9541 atagccgcca ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca
     9601 tacaatgcat cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca
     9661 ccctgtgccc gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtgc
     9721 tgaatgttga gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt
     9781 taaggtcaaa aaaaaccccc ggacctttcg gtgcgggggt cttagttcgt taaggcttga
     9841 tttctaagcc tttcctcgtc caagtgcagc cccgcacggt gggataataa tcaccaccac
     9901 gctaatcacg accaggctaa tcactcgtag aagggctgtc attttgtctt ttcttgcatc
     9961 ttgttcgaag gaatgcctaa agagttacca taggttttgt aaatagtaca agatattttt
    10021 tggccgattt ttatgatgcg tttaataatt tcaatataag ttgttgtttt ttaaaaataa
    10081 aatagtgagt aaaaaaattc aatttcagta tgaatgttgt tcccttcctc gtagttcatc
    10141 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10201 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10261 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10321 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10381 cccggcttaa cgaaggtggg cttaccagaa acaacggtaa aagaagctcg cgatcgcgtg
    10441 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctg
    10501 gctccagctg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10561 ctggcggcct cagaacagct tacagccaat aagttagatg aatatggatt agtcggagaa
    10621 ctggcgctta caggcgctct gcgtggcgtt cccggcgcaa tctccagtac aactgaagct
    10681 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10741 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10801 aagcacgctc tcgaacgccc gaaaccaact gatacagtat cccgggcgct acaacatgat
    10861 ctcagtgatg ttatcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10921 gggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    10981 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagtgc tgcgatatta
    11041 agtctggtaa acgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11101 catcacagtg catcgttaac tgcgatggta ggcggtggcg caattccagg gcccgatgaa
    11161 atttcgctgg cgcataacgg cgtgcttttt cttgatgagt tacctgaatt tgaaaggcgt
    11221 acactggatg ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacgcga
    11281 gcaaaaataa cctatccagc ccgtttccag cttgtcgcgg cgatgaatcc cagccctacc
    11341 ggacattatc agggaaacca taaccgctgc acaccagagc aaacgctgcg ttatctcaac
    11401 cgactctctg gtccctttct cgaccgcttc gatctctcac tggagatccc attaccgcct
    11461 cccggtattt tgagtaaaac ggtagtgccg ggagaaagca gcgccaccgt taaacaacgc
    11521 gtaatggccg ccagagagcg ccaatttaag cggcagaata aactgaacgc ctggctggat
    11581 agtccggaaa tacgccaatt ctgcaaactt gagagcgaag atgcgatgtg gctggaagaa
    11641 acgctgatcc atctggggtt atcgattcgt gcctagcagc ggttattgaa agttgcacga
    11701 accattgctg atattgatca gtctgacatt atcacacgtc aacatttgca ggaggcagtt
    11761 agctatcgag cgattgaccg tttgctcatc catctgcaga aactactgac ataaaaaaag
    11821 ggcatttcgc cctttttatt aatcgtcaga atcggtgtag tcttcagcac cttcaacctg
    11881 cggtttaccg ccggaaaggg tgtgaaaacg ttttggacgc ttaatacgcg tcatatactt
    11941 ggaccacacg cgttctgctt ctgtcactgg ctcacgttcg ccacggcata ctgctacgaa
    12001 gagtttctct tcctcggtaa ccggctcgcg tttgccaaga tccaactcat tgaaggcata
    12061 accatgacgc tcaagcagtt gtgcctcttt gatggtgaaa tcaccatgac gagagaatcc
    12121 acgtggataa tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct
    12181 gcacgctcct aattctttga ccgagctagt tatggcgcgg agtattagtt acgcttgaca
    12241 gagtgtaaaa caaaacattt aaatcataac gacaaataat tttgcggaga gcactgtgga
    12301 tacggaattg ttaaaaactt tcctggaagt tagccgaacg cgtcactttg gtcgagcggc
    12361 tgaatcgctc tatctgaccc agtcagcagt gagctttcga atcagacaac tggaaaatca
    12421 actgggtgtg aaccttttca cccgccacag aaacaatatc cgtttaaccg ctgccggtga
    12481 aaaactactg ccttatgcag aaacgctcat gagcacctgg caggccgccc gcaaggaggt
    12541 ggcgcatacc tcacgacata acgagttttc tatcggtgcc agcgcctcat tgtgggaatg
    12601 tatgcttaat cagtggctgg gacgtttgta tcaaaatcag gatgcccata caggcttaca
    12661 gttcgaagcg cgaattgccc aacggcagtc tctggtaaaa cagctgcatg aacgccagct
    12721 tgatcttctt attaccactg aagcgcccaa aatggacgaa tttagtagtc agttgctggg
    12781 atatttcact ttagcgcttt ataccagtgc cccttcaaaa ctaaagggag atcttaatta
    12841 tctgcgactt gagtgggggc cagattttca acagcatgag gcaggtttga tcggtgctga
    12901 cgaagtgccc attctgacaa ccagttctgc tgaactggca cagcaacaga ttgcgatgct
    12961 taatggttgc acctggctac ccgtcagctg ggcgcgtaaa aaaggcggcc tgcataccgt
    13021 tgtcgatagc acaacacttt cacggccgct ttatgccata tggctgcaaa atagcgataa
    13081 aaatgcgttg attcgcgatc ttttgaaaat taacgtgctg gatgaagtgt attaatatga
    13141 atggctggca aggatgccgg tagaaggatt tacttcggag agggttattt cagataaaaa
    13201 aaatccttag ctttcgctaa ggatgatttc tggcaggggc ggagagactc gaactcccaa
    13261 cacccggttt tggagaccgg tgctctacca attgaactac gcccctaatt ag
//
LOCUS       DAJVOY010000107        13304 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVOY010000107 DAJVOY010000000
VERSION     DAJVOY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.107"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            505..1329
                     /gene="gadX"
                     /locus_tag="OQL90_002719"
     CDS             505..1329
                     /gene="gadX"
                     /locus_tag="OQL90_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1369440.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1697..2425
                     /gene="gadW"
                     /locus_tag="OQL90_002720"
     CDS             1697..2425
                     /gene="gadW"
                     /locus_tag="OQL90_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1369441.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2788..5901)
                     /locus_tag="OQL90_002721"
     CDS             complement(2788..5901)
                     /locus_tag="OQL90_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1369442.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OQL90_002722"
     CDS             complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OQL90_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCS1369443.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7143..7421
                     /locus_tag="OQL90_002723"
     CDS             7143..7421
                     /locus_tag="OQL90_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369444.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OQL90_002724"
     CDS             complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OQL90_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1369445.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OQL90_002725"
     CDS             complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OQL90_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1369446.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9575..9907
                     /gene="hdeA"
                     /locus_tag="OQL90_002726"
     CDS             9575..9907
                     /gene="hdeA"
                     /locus_tag="OQL90_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1369447.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10023..10349
                     /gene="hdeB"
                     /locus_tag="OQL90_002727"
     CDS             10023..10349
                     /gene="hdeB"
                     /locus_tag="OQL90_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1369448.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10413..11060
                     /locus_tag="OQL90_002728"
                     /pseudo
     CDS             10413..11060
                     /locus_tag="OQL90_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11102..11632)
                     /locus_tag="OQL90_002729"
     CDS             complement(11102..11632)
                     /locus_tag="OQL90_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1369449.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OQL90_002730"
     CDS             complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OQL90_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1369450.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12709..13304)
                     /locus_tag="OQL90_002731"
                     /pseudo
     CDS             complement(12709..13304)
                     /locus_tag="OQL90_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3683 a         2891 c         2977 g         3753 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacaca cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaca
      181 gctgggcatt cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg
      241 ataagatata tgttatatga atgtttatta aatgtagcga ttgcctttaa tgtcacgaaa
      301 agggattatt tgcttgctat taatttcact gcgttcttaa acgcgttttt gtattataaa
      361 tccagtcatc ctgcccgtag aacatcacac attatcatcc tgttctcccg cttcgtttaa
      421 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaatt aacagtaata
      481 tgtttatgta atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac
      541 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggtggt
      601 gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta
      661 ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga ggcattaaat
      721 cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag agatattcct
      781 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      841 cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga gcactttata
      901 ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacaa gagtttgtac ggttatcaat
      961 aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca
     1021 agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt gcttactgag
     1081 tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat taagcgagtc
     1141 gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat
     1201 gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg
     1261 gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc ggaaggaata
     1321 agattataga attttactca gacataaaaa aaacctggca taggggaccg ggaagaggat
     1381 agtctgccgt ctccagacta ataaaccgct ataacactcc ctgttggcac gggaaacttt
     1441 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1501 acataagcta tacgctgtgc gaaaatatat tcgtgctgca tttacttatt atcaattaac
     1561 tgttatgcaa aactactttg tggataaatt ttggtcctac caaatctggc agtttttgcg
     1621 ctaagaaaca gtctggcaac atttcattag tatactgaaa ttgaaataat cgcagtatga
     1681 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
     1741 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
     1801 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
     1861 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
     1921 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
     1981 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
     2041 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
     2101 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
     2161 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
     2221 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
     2281 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
     2341 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
     2401 acattcgcta ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
     2461 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
     2521 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
     2581 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
     2641 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     2701 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     2761 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     2821 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     2881 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     2941 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     3001 ccgcgccgct tcgatgattg cccctatcgg cgttttccct tctttctgca tcatctcaac
     3061 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     3121 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     3181 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     3241 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     3301 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     3361 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     3421 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     3481 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     3541 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     3601 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     3661 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     3721 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     3781 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     3841 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     3901 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     3961 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     4021 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     4081 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     4141 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     4201 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     4261 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     4321 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     4381 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     4441 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     4501 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     4561 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     4621 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     4681 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     4741 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     4801 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     4861 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     4921 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     4981 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     5041 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     5101 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     5161 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     5221 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     5281 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     5341 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     5401 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     5461 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     5521 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     5581 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     5641 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     5701 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     5761 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     5821 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     5881 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     5941 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     6001 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     6061 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     6121 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     6181 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     6241 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     6301 cgggtcggag aatttcagtg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     6361 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     6421 gcgcaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     6481 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     6541 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     6601 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc
     6661 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     6721 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     6781 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     6841 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     6901 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     6961 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     7021 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     7081 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     7141 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     7201 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     7261 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     7321 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     7381 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     7441 cccagggtga cgatgtcgct catacgtctt agtttcaact tgtacataat gttgaatttc
     7501 tgaatcttta atgtttgttg cgaaatattg agcgtcaacg tgatattgct ttcattttta
     7561 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     7621 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     7681 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     7741 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     7801 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     7861 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     7921 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     7981 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     8041 tataagaact ttacccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     8101 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
     8161 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
     8221 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     8281 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     8341 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     8401 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     8461 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     8521 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     8581 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
     8641 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     8701 atcatagtca catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta
     8761 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     8821 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     8881 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     8941 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     9001 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     9061 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     9121 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     9181 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     9241 ataaactgga ttgctctgcg atgtttttta agcatctcca gatcaaactt caaaattgtt
     9301 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     9361 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc
     9421 agtatctgat gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt
     9481 ccatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgatttgtg
     9541 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
     9601 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
     9661 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
     9721 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
     9781 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
     9841 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
     9901 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
     9961 cttctttaaa agcagctatt cctcctgttc atatataatc tatatattga atgggttaca
    10021 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
    10081 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
    10141 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
    10201 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
    10261 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
    10321 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg
    10381 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
    10441 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgtga ggcaaaggag
    10501 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
    10561 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
    10621 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
    10681 aaaatattgt aggtttgacg acggcagcgg atatctggat gaccgccgct ataggtatgg
    10741 ttattggcag tggtatgtac gaattgggta tgtatggttc agtgatgacc ttgctggtgc
    10801 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
    10861 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
    10921 cggatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
    10981 ttcatgccac aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga
    11041 aaggcgtttc aattagctaa tctgtgtggc agcgtagtca gactcaccgt aagcctgaaa
    11101 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
    11161 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgttatt ttcagtttct
    11221 ccagaatatc ctccgttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
    11281 gcgaaagtga cacagccgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
    11341 aatcgatgat agtgatagtc cgcggaacaa aggtcaccgg cactttcccc aggcagcgtt
    11401 taatattaaa gggggagaaa ataatcacat ggacaggttt taatcgttca atttgagtga
    11461 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacaaag gcattctgat
    11521 gcaacattcc gtcgatctct tcttcgttct gtatatgaac gacattacct ttgctcaaaa
    11581 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
    11641 cctggtgcgg acattaattt tataatcagt ttgccaaagg taattaacag gacagggctt
    11701 ataataataa tagttagctt gctgtgtatt aattttatgt aatgcacgat gcagatgctg
    11761 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
    11821 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
    11881 atcccatagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
    11941 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
    12001 aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct
    12061 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc
    12121 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
    12181 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
    12241 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
    12301 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
    12361 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
    12421 tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag
    12481 ttcaattcca ttgattttgg ggcgtggcat aagtatccat cgccccataa tataatcctt
    12541 tgttttaaaa ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat
    12601 attatggtat tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt
    12661 ctatttccac cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg
    12721 tgtgtgacat tttttttggg ttaagcgtct cgaattgaaa tacttcgaac tgatattcag
    12781 acatttctgc ccatgtttga attttattgt atcgagagag tgtcgtagag gctttaactt
    12841 gcttataaca ttgtttttgg aattcttgct gatattttaa tatgtcattg tatgaaagat
    12901 tggttatcct ggcctctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta
    12961 aaaagttgtc attaatggtt aataatgttg aaaataaacc ttcgatttgt ttgtcaagat
    13021 attgtccatc tttatataat ttgggggggt agggtgttct ttatgtaaaa aatagtgtca
    13081 cctaaatttg cgtcagtgaa atttatatta agtagatctt cgaaataaaa ccttgacaag
    13141 gttagagcga acccggaaag gttctgtcct gtcatattga aatcaataaa gttggttccg
    13201 gcaaagactt tcatcctgtt tttattttca ctgtgctcaa tatataactt cgtcattttt
    13261 tgatacaggc tttgaatatc ttttatatta gttggtaagt cagc
//
LOCUS       DAJVOY010000108        13087 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVOY010000108 DAJVOY010000000
VERSION     DAJVOY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.108"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            176..1150
                     /gene="cysB"
                     /locus_tag="OQL90_002732"
     CDS             176..1150
                     /gene="cysB"
                     /locus_tag="OQL90_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1369451.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QTRYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1445..1609
                     /locus_tag="OQL90_002733"
     CDS             1445..1609
                     /locus_tag="OQL90_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1369452.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1612..1779
                     /locus_tag="OQL90_002734"
     CDS             1612..1779
                     /locus_tag="OQL90_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369453.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1893..1988
                     /locus_tag="OQL90_002735"
     CDS             1893..1988
                     /locus_tag="OQL90_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369454.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2152..4827
                     /gene="acnA"
                     /locus_tag="OQL90_002736"
                     /pseudo
     CDS             2152..4827
                     /gene="acnA"
                     /locus_tag="OQL90_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="OQL90_002737"
     CDS             complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="OQL90_002737"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1369455.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5651..6415
                     /gene="pgpB"
                     /locus_tag="OQL90_002738"
     CDS             5651..6415
                     /gene="pgpB"
                     /locus_tag="OQL90_002738"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1369456.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6564..6872
                     /gene="lapA"
                     /locus_tag="OQL90_002739"
     CDS             6564..6872
                     /gene="lapA"
                     /locus_tag="OQL90_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1369457.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6879..8048
                     /gene="lapB"
                     /locus_tag="OQL90_002740"
     CDS             6879..8048
                     /gene="lapB"
                     /locus_tag="OQL90_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1369458.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8241..8978
                     /gene="pyrF"
                     /locus_tag="OQL90_002741"
     CDS             8241..8978
                     /gene="pyrF"
                     /locus_tag="OQL90_002741"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1369459.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8978..9304
                     /gene="yciH"
                     /locus_tag="OQL90_002742"
     CDS             8978..9304
                     /gene="yciH"
                     /locus_tag="OQL90_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1369460.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="OQL90_002743"
     CDS             complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="OQL90_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1369461.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9917..10666)
                     /locus_tag="OQL90_002744"
     CDS             complement(9917..10666)
                     /locus_tag="OQL90_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1369462.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10756..10929)
                     /locus_tag="OQL90_002745"
     CDS             complement(10756..10929)
                     /locus_tag="OQL90_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369463.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="OQL90_002746"
     CDS             complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="OQL90_002746"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1369464.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3329 a         3081 c         3291 g         3386 t
ORIGIN      
        1 tcgcgaaagc ggcccttttt tattgcatat tattttttct tcacacctat acactaaggc
       61 tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt attagtcacc
      121 cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt ttaacatgaa
      181 attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg tctcatcaac
      241 agcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa tgctggaaga
      301 cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg taacgccagc
      361 agggcaagaa ataattcgta tcgctcgcga agtcctgtcg aaagtcgatg ccataaaatc
      421 ggttgccgga gagcacacct ggccggataa aggctcactg tatatcgcca ccacgcatac
      481 ccagacacgc tacgcattac caaacgtcat caaaggcttt attgagcgtt atcctcgcgt
      541 ttctttgcat atgcaccagg gctcgccgac acaaattgct gatgccgtct ctaaaggcaa
      601 tgccgatttc gctatcgcca cagaagcgct gcatctgtat gaagatttag tgatgttacc
      661 gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg caggcaaaaa
      721 agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct tcggctttac
      781 cggacgttca gaactggata ctgcctttaa ccgcgcaggg ttaacgccgc gtatcgtttt
      841 cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg gggtaggggt
      901 cattgccagc atggcggtgg atccggtcgc cgatccggac cttgtgcgcg ttgatgctca
      961 cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt ttttgcgcag
     1021 ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg tcgttgatgc
     1081 ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg tttaaagata taaaactgcc
     1141 ggaaaaataa tttcttgcgt tattttcggc accttttatg tagcgaaggt gccggaatat
     1201 attctctttt gttacttatt tttataccgt gcacaatatt tttatccctc atatttatag
     1261 ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg caaattccag ttttcaaata
     1321 tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat tcgctggatt
     1381 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
     1441 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
     1501 accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
     1561 acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac tatggtcggt
     1621 caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac agaaacgaaa
     1681 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca gcaagccttt
     1741 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
     1801 atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg agttatctca
     1861 acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
     1921 cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
     1981 ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac aaggtttctc
     2041 ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt atctttaata
     2101 ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca
     2161 accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta ccactactac
     2221 agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa gtcactcaaa
     2281 gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga agaggatatc
     2341 cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg
     2401 gcaagggtgc tgatgtagga ctttaccggc gtacctgccg ttgttgatct ggcggcaatg
     2461 cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct ctcaccggtc
     2521 gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa
     2581 gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga
     2641 aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt
     2701 aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga atggattgct
     2761 tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg ccttggcgtg
     2821 ctggggtggg gcgtgggtgg gatcgaagca gaagccgcaa tgttaggcca gccggtttcc
     2881 atgcttatcc cggacgtagt gggctttaaa cttaccggta aattacgtga aggtattacc
     2941 gccacagacc tggttctcac tgttacccaa atgctgcgca aacatggcgt ggtggggaaa
     3001 ttcgtcgaat tttatggtga tggtctggat tcactaccgc tggcggatcg cgccaccatt
     3061 gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga tgctgtaacc
     3121 ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg ttgagttggt cgaaaaatat
     3181 gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac cgatttttac cagtacgtta
     3241 gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgtcc acaggatcgc
     3301 gttgcactgc ccgatgtacc aaaagcattt gccgccagta acgaactgga agtgaatgcc
     3361 acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta tcagttacct
     3421 gatggcgctg tggtcattgc tgcgataacc tcgtgcacca acacctctaa cccaagtgtg
     3481 ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc tgggcctcaa gcggcaaccg
     3541 tgggtcaaag cgtcgctggc accgggttcg aaagtcgttt ctgattatct ggcaaaagcg
     3601 aaactgacac cgtatctcga cgaactgggg tttaaccttg tgggatacgg ttgtaccacc
     3661 tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa aaaaggcgat
     3721 ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg aaggccgtat ccatccgctg
     3781 gttaaaacta actggctggc ctcgccgccg ctggtggttg cctatgcgct ggcgggaaat
     3841 atgaatatca acctggctgc tgagcctatt ggtcatgatc gtaaaggcga cccggtttat
     3901 ctgaaagata tctggccatc ggcacaggaa attgcccgtg cggtagaaca agtctccaca
     3961 gaaatgttcc gtaaagagta cgcagaagtt tttgaaggca cagcagagtg gaaggaaatt
     4021 aacgtcacac gatccgatac ctacggttgg caggaggatt caacctatat tcgcttatcg
     4081 cctttctttg atgaaatgca ggcaacacca gcacctgtgg aagatattca cggtgcgcgg
     4141 atcctcgcaa tgctggggga ttcagtcacc actgaccata tctctccggc gggcagtatt
     4201 aagcccgaca gcccggcggg tcgatatcta caaggtcggg gtgttgagcg aaaagacttt
     4261 aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac cttcgccaat
     4321 attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg gcatttacct
     4381 gacagcgatg ttgtctctat ctatgatgcc gcgatgcgct ataagcagga gcaaacgccg
     4441 ctggcggtga ttgccgggaa agagtatgga tcaggctcca gccgtgactg gacggcaaaa
     4501 ggtccgcgtc tgcttggtat tcgtgtggtg attgccgaat cgtttgaacg aattcaccgt
     4561 tcgaatttaa ttggcatggg catcttgccg ctggaatttc cgcaaggcgt gacgcgtaaa
     4621 acgttagggc taaccgggga agagaagatt gatattggcg acctgcaaaa tctacaaccc
     4681 ggcgcgacgg ttccggtgac gcttacgcgc gcggatggta gccaggaagt cgtaccctgc
     4741 cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg cattttgcat
     4801 tatgtcattc gtaatatgct gaagtaacaa gtatttgctt gccggttatt ttgcttccgg
     4861 caagcaaatt aattccacaa tgcgagaggg ttatttgttc agcaaatgac ccattttctc
     4921 ggctttggta tcgagataat gttcgttatt ggggttacga cctacaatca atggtacgcg
     4981 ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt tattggttaa
     5041 caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag tgaagtcgcg
     5101 ctcatcagcg gcgaagccta actggtggtt agcctctacg gtatcgtaac cttgatcctg
     5161 cagtgcgtaa gcgcggattt tattcagcag accaatgtta cgaccttcct gacggtgata
     5221 cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat gccgcttcga gctggaagcc
     5281 acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat ggacgcgcgc
     5341 aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat cgtgtccggt
     5401 tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca gtttggcttc
     5461 tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta ttggcttatt
     5521 ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac ggaatggcac
     5581 aaatctgtca atttttcctg gaactggcgt tttcagttat gattgtggga cttatcaaaa
     5641 aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac tattgcttgt
     5701 catgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac aaagttggct
     5761 actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg tcattacaca
     5821 tttgatttta ttcggctggt ttctctggtg tctgcgtttt cgcattaagg ctgcctttgt
     5881 attatttgcc attctggcgg ccgcaatcct tgtgggacaa ggcgttaaat cctggatcaa
     5941 agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaacac atcatattcc
     6001 ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga aagaacagtt
     6061 ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcagaaag agacggggtt
     6121 tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gcactgctgg ccgttggttt
     6181 gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg ctgctctggg caacgggagt
     6241 catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtag tagctacatt
     6301 gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt gtgggccatt
     6361 aacaccacct gcggaagaaa atcgcgaaat agcgcaacga gaacaagaaa gttaacgctg
     6421 gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc catttcgcag
     6481 acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa tttattcgct
     6541 ctaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg gtgttagcga
     6601 tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca acaggtgacg tttaattatc
     6661 tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt gctgcggggt
     6721 ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg agttcgtgtt tccctggcgc
     6781 gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact gacgtggctg
     6841 tagtgccgca ctcgtcagcg gcgaaggaat aactttctat gctggagttg ttgtttctgc
     6901 ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg caacaaaaca
     6961 agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac ttcctgctta
     7021 gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag gatacaggca
     7081 ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa gttgatcgcg
     7141 ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct gacctatgaa cagcgtctgt
     7201 tggcgattca acaactaggg cgtgattaca tggccgccgg gttatacgac cgcgcggaag
     7261 acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgctctg caacagttgc
     7321 tacaaatcta ccaggctacc agtgagtggc agaaagcaat tgatgttgcc gaacgcctgg
     7381 tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt gagttagccc
     7441 tgcagcatat ggccagcgac gatctcgatc gtgccatgac cttgctaaaa aaaggggcgg
     7501 cggcagataa aaacagagcc cgcgtatcca tcatgatggg acgcgtgttt atggcgaaag
     7561 gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat atcccaggac agagaactgg
     7621 tcagcgaaac gctggaaatg ttgcaaacct gctatcagca gttgggtaaa actgccgaat
     7681 gggcagaatt cctgcaacgc gcggtggaag agaacaccgg tgccgatgct gaattgatgc
     7741 ttgctgatat catcgaagcg cgcgacggta gtgaggccgc acaggtctat attacgcgcc
     7801 agcttcagcg tcatccgacc atgcgtgtgt tccataagtt aatggattac cacttaaatg
     7861 aagcggaaga agggcgtgcc aaagagagcc tgatggtgct gcgtgacatg gttggcgaaa
     7921 aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca tacaccctct
     7981 actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc ggtcttgatg
     8041 gcctgtaatt tttaaaaaaa tccgacttta gttacaacat gctagttatt gatgttccat
     8101 tgtgctccgg caacgacgga gcagaaaaag cctgccaggg gaaaaatcgc aactgttaat
     8161 tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc
     8221 atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga
     8281 attctcctgt ggttgttgcc cttgattatc ataatcgtga tgacgcgctg tcctttgtcg
     8341 acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt acattgtttg
     8401 ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt gacctgaaat
     8461 tccacgatat tcctaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt
     8521 ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac
     8581 tggttccgtt tggcaaagat gcaccgcttt tgattgctgt aacagtgttg accagcatgg
     8641 aagctagcga cctggccgat cttggcgtga cactgtcacc tgcagattat gcagaacgtc
     8701 tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg
     8761 tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacaccgggc attcgtccgc
     8821 aggggagtga tgctggtgac cagcgccgca ttatgacgcc agaacaggcg ttggcggctg
     8881 gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcaaacac
     8941 tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct
     9001 ggtctactca acggaaaccg gacgtattga tgagccaaaa gcggccccag tacgccctaa
     9061 aggcgatggt gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta agggcgtttg
     9121 cctgattacc ggtatcgatc tcgatgatgc cgaactgaca aaacttgcag cggaactgaa
     9181 gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt attgaaatcc agggcgataa
     9241 gcgtgattta ttaaaatcac tacttgaagc gaaagggatg aaggtaaaac tcgcaggcgg
     9301 ttaacataaa aagccacgga tatatccgtg gctttcgaat attttactgt gtgtattatt
     9361 cagttatttc gtactgaaca aaatcaagcg tattataatt gcgagtcgat aatcgacatt
     9421 tatttacgat tatttaccga cctggtgacc aataacacca ccgacagctg caccacctaa
     9481 tgtacccaac gtactgccat cggtcagtac tgcaccgcct aatgcccctg cacccgcgcc
     9541 gattgcggtg ttgcggtccc gtttagacca gttagaacag gcactcagag acattgccaa
     9601 agtaattgcc agaacagccg cggtcatttt tttgctcgtt acaaacataa tactctctcc
     9661 tgaatttatg attcacggaa gtaagctctc tataactata atagctaaga ataagcctgg
     9721 tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata tcacgcaggt gataatgact
     9781 tcctgttata tcgctgataa taattttata tcttaagcgt gttaataaca ggtaaatagt
     9841 cttaattatc aaccaggaat catcttagag cggatgattt gccaaactgc aaatcatccg
     9901 tagagaaggg aaatggttaa atgtcaacga cgtgaacggt gagtttactg tgctcaagat
     9961 gcatcagatc gctggcgcgt attttcgaat cggtaatcac gcgattgaat cgctcaacgg
    10021 gaccgatgga gtatgaatgt acagcaccaa atttcgagct gtcagtcagg actatcactt
    10081 cgcactcttt ttccagcacg gcattgacca catcggtacg catcatatcg cgaccggtga
    10141 atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa tgcacctgct
    10201 ggatgcactg gcgtgtcaac gggccaacca tactttcgct ttttttctgg tacacgccac
    10261 cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag ctgctgaccg
    10321 tgatgatagt gacatttttc ttctgctccc ccagagtccg ggcgagcagg gcattgctgc
    10381 tgccattttc gataaagatg gtttcgcccg gttgaaccag tgacgcggca aactcggcga
    10441 gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca ctatcaagcg
    10501 aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg ttgagatcct
    10561 ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg ctaacctgac
    10621 cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatcgct gtaattctta
    10681 taacgttata atactaaata aaaaaatact gacgcgccgg gcatcctcaa ataggaaaac
    10741 ctgaaggcac gattgttaaa tctcccacgg tgccttcggg atgtctggcg tagattcagc
    10801 aacctggcgc aagagttctg ctttaagtat ctcgagatta tggatcgcag agtggtaatc
    10861 gcctgccaca aggatatcta acacaatatc aatacgttcg gcaacgcgct gggcatcaaa
    10921 ttcagacatc aaggacatcc ttatagcaaa agtggaaaat gaataatgca aaaattgccg
    10981 ctaaaagaga agtgtttaac agcaacggct aattatcatc caggaatacg atatataatg
    11041 acgggatata gcgctaagta tatatattca tctacttatg cgcgcttcag atagcgttta
    11101 taccagcgtt cgaaggcgac ggcgggcatc ggtttggcaa acaaaaatcc ttgccgctca
    11161 ttgatcccgt tcttggttaa aaaagcatct tcctttgcac tctctacacc ttcggcgatc
    11221 acctgaagat tcaatgcctg ggccacagcg acgatcgccc ggaccagtga ctgcgagaca
    11281 ggttgtttgt gaatatctcg aacaaaaacc tggtcaagtt tgatggcatc gatcggaaag
    11341 cgcgccagtt gcgaaagtga agagtagccg gtaccaaaat catccagatg cacttgcgca
    11401 cctagttggc taaattgttg aataacagac agtgccagtt catcattctc aatcagacaa
    11461 ctctctgtca gttcaacatc tatagggcag tattcaaaat tgagttcctg gagaacctgt
    11521 ttcagggcgg tgaaaatggt ttgatcggcg agctgacgtg cagaaatatt taccgccact
    11581 cgcaggttta tgcctttatc ccgccacttt gccacctggc gtacgacatc gagaatcacc
    11641 caacggccta aaggcacaat tagccctgac tcttcggcgt aggaaatgaa gtccaacggt
    11701 ggaatcaacc cacgttcagg tgactgccaa cgtactagtg cttccagact gcgcacttcg
    11761 ccacgccagg tgattttcgg ttgatagtga ataaccaact gatcgttttc cagtgcttta
    11821 cgcaagttgg tatccagcca gagatattca aatacccgtt gattcatttc tggggtaaaa
    11881 acgcaaaatt gtcctcgtcc gccttccttc gctgtgtaca ttgctgtgtc ggcgtgacga
    11941 ataatagccg tgctgtctga accatgttcg ggagagagtg caatacctac tgaacagctg
    12001 gtataaactt caattaaacc aatgcgaaag gggagccgta agcgggtcaa aattcgtgat
    12061 gccattgctt ccagcgcgct ttgtgaggtg ttggatgcca gtaccagaaa ctcatcccca
    12121 cctggacgcg ccaacacctg gtcatgttcg agacagctta aaatagccaa tgacacgtcg
    12181 cgtaataact ggtcaccaaa caaatgccca taggcgtcgt tgaccttttt gaaattatcc
    12241 aaatcaagat acacaacccc aactttattg ttatctgcat gattaatagc gtgatcgatt
    12301 aaatcctgca ttgcgttacg attcggcagt ccggtgatac tgtcggtatt tgccagaata
    12361 cgcagtcgct cctgagcgcg gcgctcttcg gtaatgtcgg tgccggaaca gattaaaaaa
    12421 atctcgcttt tgccactgcc gctgtggaca aatttattgc gaaacagaaa cagccgctgg
    12481 cctttacatg ttggtatcca cagttcgact tcatatgcat tgccgcttcg aaaaaataca
    12541 cggttattgc gcctggatgc cgcagcttca cgacggctca taaacagttt aaacacgctt
    12601 tggccaatga cgtcgtgttc tttcaaccca gtgtaatctt cacataaccg attgaagcgt
    12661 tggatattcc cccggctgtc gagaatcact attgcggaat gtgcttcaga aactacctgc
    12721 tcagcaaagg aaagcccgtg tgataaatca cgagcaactg ccggtgtgtc atgccatgca
    12781 gacgcgttgc cagcccattc ccgtttattg atttttcgtc ctaccagatg cacagaaaga
    12841 tcgctctcat cgacggtcag actcatcatc aggctggagg tgatgaccgt catttccctg
    12901 atgcgagcag cctgttcggc agataactgc aaagtacgat caggttctgt ggtttcggtg
    12961 gtagaaaagc gcaaaacatc gctgctttcc gtcaaccgcc agtatggatt gtgcgatccg
    13021 agaaagttgt acaacgttgt ggactcccta acggttttca tgatgcatat cctccggtta
    13081 aataaaa
//
LOCUS       DAJVOY010000109        13014 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVOY010000109 DAJVOY010000000
VERSION     DAJVOY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.109"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            4..1632
                     /locus_tag="OQL90_002747"
     CDS             4..1632
                     /locus_tag="OQL90_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCS1369465.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKQSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1871..1930
                     /locus_tag="OQL90_002748"
     CDS             1871..1930
                     /locus_tag="OQL90_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1369466.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2247..2306
                     /gene="ibsE"
                     /locus_tag="OQL90_002749"
     CDS             2247..2306
                     /gene="ibsE"
                     /locus_tag="OQL90_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS1369467.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="OQL90_002750"
     CDS             complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="OQL90_002750"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1369468.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="OQL90_002751"
     CDS             complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="OQL90_002751"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1369469.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6770..8071)
                     /gene="ygiF"
                     /locus_tag="OQL90_002752"
     CDS             complement(6770..8071)
                     /gene="ygiF"
                     /locus_tag="OQL90_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1369470.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8313..8933
                     /locus_tag="OQL90_002753"
     CDS             8313..8933
                     /locus_tag="OQL90_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1369471.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            8997..10235
                     /gene="cca"
                     /locus_tag="OQL90_002754"
     CDS             8997..10235
                     /gene="cca"
                     /locus_tag="OQL90_002754"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1369472.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="OQL90_002755"
     CDS             complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="OQL90_002755"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1369473.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11304..11672)
                     /gene="folB"
                     /locus_tag="OQL90_002756"
     CDS             complement(11304..11672)
                     /gene="folB"
                     /locus_tag="OQL90_002756"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1369474.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11777..12394
                     /gene="plsY"
                     /locus_tag="OQL90_002757"
     CDS             11777..12394
                     /gene="plsY"
                     /locus_tag="OQL90_002757"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1369475.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12407..>13014)
                     /gene="ttdR"
                     /locus_tag="OQL90_002758"
                     /gene_synonym="dan"
     CDS             complement(12407..>13014)
                     /gene="ttdR"
                     /locus_tag="OQL90_002758"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCS1369476.1"
                     /translation="HIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEI
                     PDYYIAHLLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNG
                     QEKKSVKVSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSA
                     NIWAVYREPLYRSMKLRVCVEFLVAWCQQRLGKPDEGYQVM"
BASE COUNT         3144 a         3654 c         3264 g         2952 t
ORIGIN      
        1 tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgct
       61 aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa
      121 aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc
      181 aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa
      241 gatcgtatgc gtgtcgatgt agtagtcgct ttctttgtac gggtaaaaca gtctgtagaa
      301 ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga agacttacgt
      361 atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg
      421 catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa
      481 gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt taaccagacc
      541 tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgatt
      601 caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg
      661 gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg
      721 tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt
      781 attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat tctggctgaa
      841 cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag aaaggttgag
      901 gctgaacgtg aagttcgtat taaagagatc gaacagcagc aggtcaccga aatcgccaac
      961 cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt
     1021 gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac cactcgccag
     1081 accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa
     1141 accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag
     1201 gcggcggcta tcgttaagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa
     1261 gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa
     1321 ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt tgagccgatg
     1381 aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg
     1441 ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc attatcagcc
     1501 gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt
     1561 tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc cgtcgccgaa
     1621 aacgtagaat aaattttcag ctatgaacgt ccctggcaca aaaagccggg gattttttat
     1681 atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg cgtgaccgct
     1741 cggggaaggc aaaactgcct ctgggaaagc gttgctcgag gtccggcgct catcaacaat
     1801 cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg ccttacagaa
     1861 ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg taagtttcgc
     1921 agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt
     1981 taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct agcccaaaaa
     2041 gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc
     2101 gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc
     2161 ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg
     2221 aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt
     2281 tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat cctcccttac
     2341 ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc ctgctacgaa
     2401 gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgatga
     2461 tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg ccgttggccc
     2521 agacttcttt actcccggcg atctcttctg gtttatagtc gccgcccttc accagtaaat
     2581 ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac accacccagt
     2641 cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta acggggcggg
     2701 aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc agacggtcac
     2761 ccagtttgcg agcatttgcc agataggaga cgtgcccggc atgcaggatg tcaaagacac
     2821 cgttggtcat taccaccttt tcgccgcgtt tacgcgctgc cgctacggcc agcttcagtt
     2881 cctcttcggt catcacgcca aagccggtat ctgcgcgccc acgcactgca ttttccagct
     2941 cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc gccgcattgg
     3001 caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa
     3061 tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt ggcatatgca
     3121 gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc actaacagag
     3181 ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct
     3241 tacatttacc gacaacagct tcaaattccg agagattcgg cgttaacagc gtagcgccgc
     3301 ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc gctttacgcg
     3361 ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca
     3421 gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc ggatcaacgc
     3481 cttcgaaacc ttcttcgaaa tccagacgga tcagctgttg gttgcgggaa agtacccgta
     3541 atttggtgat ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg
     3601 ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc cgtcaacccg accaggcgtg
     3661 cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc ggacgttctt
     3721 cgatggtatt cactttaacc acgggcaccg gcgcttccgg cgagatacgg ctggtgggac
     3781 cgtaccagta acgatccagc attacatcac caaccaccat cactcctgca cgttcaaact
     3841 ctgacagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac
     3901 catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagat cacgctctgc
     3961 ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat gatgaagttc
     4021 atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt ccataatgtc
     4081 gttttgcgcc agcagctcca gaatacgcac gttgtctgac cagcgcgtta acttcggttt
     4141 ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg taattccgcc
     4201 ttcatcggct ttgatatcaa agcgattacg atgtttattg ccgagatgag cgcgcatttt
     4261 ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tcacgcggca gcatcataat
     4321 ctcgcgacgc actgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacacgcgc
     4381 acgcaccagc gcctgatgct cccacgtcca ggcctcgttt ttctgataat cagcaaatgc
     4441 ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgtg catccacttc
     4501 atacaaaatg ccggaagagg ttcgcgtatt gaacagatgc ataatgcgtt gcgccagacg
     4561 cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat ccattgggca
     4621 atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc cgcccagctt
     4681 gccgtagccg accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat ttggcttacc
     4741 gtagcgggcc accatttgaa cccacgcctg ctgaacgacg gcatctatca tagcctctgc
     4801 cagccaggtt aagtgatcgc tcactttcat caccggtagc gtaccagcga tatccgctgc
     4861 ggcgatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct gttgctcttc
     4921 gtcatcttcc ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg catcggtcgc
     4981 cgtcggctgg taaagagtgt ttggatcgag caattcatcc agcaataatg gataacgcgc
     5041 cagctggctg gcaatcatcg gcgatgcggc acacagagaa atcaaatgtt taagcgccgc
     5101 agggaattca ctgagcaatt ctaaataggt ggtgcgggta acaatcccca ccagcaaggc
     5161 ggtaatgcgc gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac ttagcagatg
     5221 tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atagtacgct tatccagctc
     5281 tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga tcatcctcgc taagatgcgc
     5341 cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc
     5401 cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat taaacacccg
     5461 gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt cagcaaagtc
     5521 catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgttt gggtttgttc
     5581 gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatatg ccactcgcaa
     5641 ttgttcagca tcgttttcag aaagcagatg cagcgcggca atgacgctga gcgttggtag
     5701 taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga acacctgaac
     5761 gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg tcaaaccacg
     5821 tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa tcacgctgaa
     5881 atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt tagcatagac
     5941 gccttcgcta tcgcccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc
     6001 ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc cactttcgcc
     6061 aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccaa
     6121 cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg
     6181 tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat ctgaggagaa
     6241 attcagttcc ccaccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc
     6301 ctgcgcattg cacggcgttc cccactcgcg gcagcaagcg tcatacagcc agtcacgcgc
     6361 ggcgacaatc agcgtctccg ccagatggct gagctgctgc aatatactct cttcagtaac
     6421 caaagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga atagccgcag
     6481 ctcgcgcatt aacccggctt cgtcactcac attgctgagc gcctcctgca accatgccgc
     6541 gtaatgctgc cattcatcag cctgcggcga ttggctttcc agttccgtca gccactctgg
     6601 atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact tcgcctgtgc
     6661 gctaagtgat ttctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg
     6721 ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc
     6781 cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcaa
     6841 tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc caggcttgcg
     6901 cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca cgcgtcagac
     6961 gtggcaactg gtcacggtag cgatcgccca acggctggca gaaaacgctt ttcagttcag
     7021 cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctc attttactct
     7081 gcgctttggc atctaaaaac ggctgccagg ctttgcttac cagccattcg gtcaacgcca
     7141 gcttcgccat tgccgttttg gtagagtaga ccgccgtcac ggcagaaacc gcagaagcga
     7201 tggtcgcttc gcattgagtc agcaaatcgc gtaagtgagt gctcgctttg cgcggcacaa
     7261 taccaccgaa cagcatcagt gtatgacgga ccagaccaat ggctgccagc acctgttctt
     7321 tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca
     7381 gctcgagcgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca tgcaaaatgg
     7441 tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgccgctt
     7501 tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca
     7561 ccgcgcgcgt gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt
     7621 cccccgcttt cacctccccc tgatcgagag cgatttcaat tcggctgtca tcgaccgcca
     7681 ccagccattt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat
     7741 cggcgggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg tcgagcgtcg
     7801 gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg
     7861 caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag cccatatcgt
     7921 gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat
     7981 ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa
     8041 caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta tgtcacataa
     8101 gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca
     8161 aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga
     8221 cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc
     8281 gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa
     8341 ctttactcgc acttagcgcc actgccgtct cacacgctga agaaacgcgc tatgtttctg
     8401 atgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg
     8461 ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg
     8521 tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc actgagccaa
     8581 gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc gataaactca
     8641 ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga
     8701 gcgacagcgt gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac gagctgattg
     8761 tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga tgacaaacag cgcaccatca
     8821 tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg ctcggtctgg
     8881 tactgccaca cctgatccca acgcgcaaac gcaaagagcg ctggatgaac taaatcgcct
     8941 tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat gatggcgtga
     9001 agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca
     9061 gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg
     9121 taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat gcgctggcac
     9181 gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc gcaccggatg
     9241 tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg gcccaggacg
     9301 ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc
     9361 gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg gcgcgttttg
     9421 ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg ctgatgcgcg
     9481 agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg aaagagacgg
     9541 aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggtg
     9601 cacttcgcgt tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt
     9661 ggcatccgga aatcgacacc ggtattcata ccttaatgac gctctcaatg gcggcgatgc
     9721 tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt aaaggactga
     9781 cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt aagttagtgg
     9841 aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtgg
     9901 ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc atcgtcaaat
     9961 tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca
    10021 gcgaggctga cgtacgcggc agaaccggtt ttgaatcagc ggactacccg caaggccgtt
    10081 ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc gttgaagcgg
    10141 gatttaaagg tgtggagatt cgcgaggagc tgacccgacg gcggattgcg gcggtagcca
    10201 gttggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg ttaacacgaa
    10261 ccatcccctc gccccttcgg ggagagggtg aggggaacag accagcaccg gtgcgaacaa
    10321 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    10381 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    10441 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    10501 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc ggttgtcagg
    10561 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    10621 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    10681 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    10741 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    10801 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    10861 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    10921 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    10981 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    11041 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    11101 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    11161 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    11221 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    11281 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    11341 attacaccaa cattcgccgc ccgcgccact gcgcctggct tgctgagttt gatgcgcacc
    11401 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    11461 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    11521 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    11581 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    11641 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    11701 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    11761 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    11821 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    11881 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    11941 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    12001 attaggtgtc agccccttct ggctaggctt aattgccatc gccgcctgtc ttggacacat
    12061 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    12121 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    12181 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    12241 ctggtggttt aagccacaat tcaccttccc agtttcgatg ctctcttgcc tgatcctgct
    12301 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    12361 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    12421 agccttcatc gggcttgccc agccgttgct ggcaccatgc caccagaaat tccacgcaga
    12481 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    12541 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    12601 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    12661 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    12721 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    12781 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    12841 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    12901 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    12961 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggc
//
LOCUS       DAJVOY010000110        12942 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVOY010000110 DAJVOY010000000
VERSION     DAJVOY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.110"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQL90_002759"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQL90_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1369477.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OQL90_002760"
     CDS             1946..2230
                     /locus_tag="OQL90_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1369478.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OQL90_002761"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OQL90_002761"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1369479.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGAWMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OQL90_002762"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OQL90_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS1369480.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OQL90_002763"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OQL90_002763"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1369481.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7227..7487
                     /locus_tag="OQL90_002764"
     CDS             7227..7487
                     /locus_tag="OQL90_002764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1369482.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            7494..7790
                     /locus_tag="OQL90_002765"
     CDS             7494..7790
                     /locus_tag="OQL90_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1369483.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7872..10514
                     /gene="yfcU"
                     /locus_tag="OQL90_002766"
     CDS             7872..10514
                     /gene="yfcU"
                     /locus_tag="OQL90_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCS1369484.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            10534..11286
                     /locus_tag="OQL90_002767"
     CDS             10534..11286
                     /locus_tag="OQL90_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1369485.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            11302..11811
                     /locus_tag="OQL90_002768"
     CDS             11302..11811
                     /locus_tag="OQL90_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1369486.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            11808..12296
                     /locus_tag="OQL90_002769"
     CDS             11808..12296
                     /locus_tag="OQL90_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1369487.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            12293..12832
                     /locus_tag="OQL90_002770"
                     /pseudo
     CDS             12293..12832
                     /locus_tag="OQL90_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         3256 a         3084 c         3439 g         3163 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttcgtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgttgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgttgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgttgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatggttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgcgcgat ttaatgcccg taccgcctgc agtagcagaa
     2941 tattctaccg gcttgcggat gggtgacacc gcagagcaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgttc
     3361 ggtccggcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacaat gagcgatctg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgcttg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgtgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgacgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat cggcaactgc aaaacggtgc
     3961 aagaagcgga agctctggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg tggtgggctg gagttggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagcacaac attagagatg atcctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagtc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggttatgac gccgaagctc gggcgtttgg cgaactggcg atgacgccac
     4561 aatcgcaggc gctgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattgaaaca acagatggtg gcggaagttg agcaaaattg cgccgctcat accatctttg
     4981 cttcgaatac gtcatcttta ccgattggtg atatcgccgc tcacgccacg cgacctgagc
     5041 aagttatcgg cctgcatttc ttcagtccgg tggaaaaaat gccgctggtg gagattattc
     5101 ctcatgcggg tacatcagcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccaaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gtccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggtgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgacctgca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaatgggcg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag ccctcgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggtac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgtgttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaacctca ctactatcga agactctcct tatctataaa
     6661 taaaacactt tctacattca ttcggaaatt attcttctgc acgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagtt gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt
     6841 gtttatatat tatattgtca aaagtggata aatgccaatc gcattatata ttcattgttt
     6901 gttttttaag ctgttgatat tcattattga tatactttct attttttcta aatacttatt
     6961 ccgtattttt taaggatgtc cttaagataa tctttcttct gtcaatatat taattaaatc
     7021 tacatttact ctttagtaaa attaaattgc atcaggggtg tggaaacatc actatgcggc
     7081 cccacaccaa cgaatacaat tttctacgac ctgttctttt tggatcgcat gccgtattca
     7141 atggctgggt ataaacattg gctattgact gaatcaatgt attgcaatgt ttatattttt
     7201 aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct attgctgcgg
     7261 caatggtgat gggcgtgttc acctctacgg caacaatcgc tgcgggtaac aacggtactg
     7321 cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac gatcacaaac
     7381 tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc accaccacgc
     7441 cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tacttaggaa acaatgacca
     7501 ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc attttcaata
     7561 ccgataccgg tgcggcattt aacaatgtta gcctggcgat cggtgactct ctgggtacct
     7621 cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct actaataaag
     7681 gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct gatgcgccag
     7741 atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa ggcatacccc
     7801 catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa taaaaacgga
     7861 tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg tggtgcattg
     7921 cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt cagtttgatt
     7981 cccgttttct ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt tccagccagg
     8041 gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag ccattggcgg
     8101 aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc tatgcttgtc
     8161 tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaaa aatctgcaat
     8221 ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa attaaggctg
     8281 atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa tatacttatc
     8341 ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc gcggactaca
     8401 gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac gagatcagcg
     8461 gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac tggcagacta
     8521 actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa actcaaaaaa
     8581 aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa gccaaactgg
     8641 cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat gttggtggta
     8701 gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg ccagacattt
     8761 ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt gtgatttacg
     8821 aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct gtctccggta
     8881 cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac atcagcaccg
     8941 cctcgatgcc atacctcact cgtccaggtc agattcgcta taagatcatg atgggccgtc
     9001 cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa gcttcctggg
     9061 ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac tatcagtctg
     9121 cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt gatgttactc
     9181 actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg gacggtaact
     9241 ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt acttttgctg
     9301 gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat gccagtgaca
     9361 gcggaatggt acgcacgggc aacgacaaag agatgtacac cgccacttat aaccagaact
     9421 tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac tgggatcgcg
     9481 aggagcagat aaactacaac atcatgctct cgcactattt caatatgggc agtattcgta
     9541 atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc gacaaaggca
     9601 tgtacatttc gctcagtatg ccgtggggcg acaacagtac cgttagctat aacggtaact
     9661 atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat gacgcgactc
     9721 actatcagtt gaacgtcggc accagtgaca aacacaccag cgttgacggc tattacagcc
     9781 atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg caatacacct
     9841 ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc gcacttcacc
     9901 gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt gccgatgttc
     9961 cggtggaagg taacggggct gctgtttata ccaatatgtt tggtaaagcc gtcgtttctg
    10021 acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacagattg cctgaaaacg
    10081 ctgaagcaac ccagtcggtg gtgcaagcca cgctaactga aggtgccatt ggctaccgta
    10141 aatttgctgt cattagcgga caaaaagcga tggcggtgct gcgcctgagc gacggcagcc
    10201 atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc cttgtcgatg
    10261 atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt gtgttctgga
    10321 gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat ctgtttaacg
    10381 gcttgttact gccatgccag cataaaggca atgtagcacc tatcacttcg ccggcggtca
    10441 aaccggcgat tcaggaacag acacagcggg tgacgccaac ggaacctccg acttcaattt
    10501 cagtaaacca gtaacgtgat taaggaatga tccatgttta atctgactaa cactgcaaaa
    10561 atcgttgtcc cggcactggc gctgctggcg acagcggtca gtttctccag ccacgcttcg
    10621 gtgacgcccg acaggacacg gctggtgttt aacgaaagcg acaagtccat cagcgtcacg
    10681 ctgcgcaaca acgatcccac actgccttat ctggcgcaga gctggataga ggatgcaaag
    10741 ggcaacaaaa tcagctctcc gcttgcggtg cttccgccag tgcagcgcat cgatgcgatg
    10801 atgaacggcc aggtgaaggt gcaggggctg ccggatatca acaaactgcc ggccgaccgc
    10861 gagagcgtgt tctatttcaa cgtgcgcgaa attccgccga aatcaaataa accgaacaca
    10921 ctgcagattg cgctgcagac gcgtatcaaa ctgttctggc gaccgaaggc gctggagaac
    10981 accagcatga aaagtccgtg gcagcataag gtgacgctca tccgtaacgg ccaggacttc
    11041 accgtcaata acccgacgcc gtactacgtg atcatcagca acgccagcgc taaaaaaggc
    11101 ggcaacccgc cggcgggatt cacaccgctg atgatggagc cgaaaacctc cgcaccgctg
    11161 aatgcgaaga tggacagcgt gccggtgctg acttacatca acgactacgg cgcgcggatg
    11221 ccgctgtact tcaaatgcac cggcaacagc tgccaggtgg atgaagagca gagccgcaaa
    11281 ggctgatgcc gggagggact tatgagtcac aagtttgtag cgggcgcagg gttgctggtg
    11341 ctggccggat ggggcatcag cggtttggct gttgctggca gtgatatcaa acaggtggat
    11401 ctgacgctgc gaatcctagt cgatggaccg ccgccttgct ccgtgaaggg aagcgctgtg
    11461 aaatttggca atgtgataat caacaggatc gatggtacca attaccaaca ggatgcgaaa
    11521 tacaccctca actgcggtaa taaagtcagc gatgacttac ggatgcagct caaaggcaat
    11581 accgccgtga tcaacggaga aacggttctc agcacggata tcgccggcct gggggtgcgg
    11641 atagaaaacg ccgccgacaa ctccctgttt gcagttggtg aaaataactg gacgcctttt
    11701 aatgtcaaca ctcagcctca gctgaaagcg gtgccggtaa aacagagcgg ggtgcaactg
    11761 actccgtcgg agtttaacgc cacgatgacc atggtggtgg attaccaatg aaaaaattcc
    11821 tgctcctttt actgtgcgct ggttgcgggg ctgtgctggc ggcggaagat gacatcacat
    11881 tccacggtac gctgatgtcg ccgcccgcct gtagcatcag cggcgggcaa accatcgaag
    11941 tgcaatttcg cgatctcatt atcgacagca ttgatggcaa ttatggtcgt cagaaagtgg
    12001 actacgaact gagctgcgac tcggatgtcc gcgatcccga gtggaatatg acactctcct
    12061 ggacggggac tgccacctcc tacaacgact ctgctattga gacggatgta ccgggcttcg
    12121 gaattgagct tcagcatgac ggtcagcgtt ttaagctcaa cgagccgctt tccattaacg
    12181 ccaccgattt tagccagaag ccaaagctgg aggcggtacc ggtcaaagcg gctgatgcgg
    12241 tgctgacgga cacgaccttt tctgcttacg caaccttacg ggtggattac caatgaaaac
    12301 agcacacatt tattcacccg gttatgcgct gttagcaggc ctgctgctga ccgccagcac
    12361 aggagcacag gcagtcgaca acaacctgca tttttacggc aacctgctga gtaaatcctg
    12421 cacgctggtg gtggatggtg ccaacctggc agaaatccat tttcccaccg tcagcaaccg
    12481 ggatttgatg gtatctgggc agtccgcgca tctgccggtt gtgtttaagc tgaaagactg
    12541 caaagggccg gcgggataca acgtcaaagt cacgctgagc ggcaccgaag acagcggtca
    12601 gccgggtttt ctggcagtcg atgacacctc caccgcgcag ggcgtgggga tagggatgga
    12661 aacgctcgac ggcaagcggg tggatattaa caacaccacc ggggcgacct ttgcgcttac
    12721 caacggcaac aatgatatta atttccgggc ctggatctag gcgaaaagcg gtcgtgatgt
    12781 aaccatcggt gatttcaccg ccagcctgac agcaactttt gagtatattt aaaatgaaaa
    12841 tccttcgtca tgttttatgt cttttattgc tgttaatgtt caatacatgc ggccaggcgg
    12901 cgacgaaagt tggtgagtta tatgtgccag ctggtgccag tt
//
LOCUS       DAJVOY010000111        12921 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVOY010000111 DAJVOY010000000
VERSION     DAJVOY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.111"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..881)
                     /gene="rarD"
                     /locus_tag="OQL90_002771"
     CDS             complement(<1..881)
                     /gene="rarD"
                     /locus_tag="OQL90_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1369488.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRR"
     gene            complement(933..1406)
                     /locus_tag="OQL90_002772"
     CDS             complement(933..1406)
                     /locus_tag="OQL90_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1369489.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATATYMVG"
     gene            1571..2440
                     /gene="pldA"
                     /locus_tag="OQL90_002773"
     CDS             1571..2440
                     /gene="pldA"
                     /locus_tag="OQL90_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1369490.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2573..4402
                     /gene="recQ"
                     /locus_tag="OQL90_002774"
     CDS             2573..4402
                     /gene="recQ"
                     /locus_tag="OQL90_002774"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1369491.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4466..5086
                     /gene="rhtC"
                     /locus_tag="OQL90_002775"
     CDS             4466..5086
                     /gene="rhtC"
                     /locus_tag="OQL90_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1369492.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5148..5768)
                     /gene="rhtB"
                     /locus_tag="OQL90_002776"
     CDS             complement(5148..5768)
                     /gene="rhtB"
                     /locus_tag="OQL90_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1369493.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5879..6901
                     /gene="pldB"
                     /locus_tag="OQL90_002777"
     CDS             5879..6901
                     /gene="pldB"
                     /locus_tag="OQL90_002777"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1369494.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6909..7709
                     /gene="yigL"
                     /locus_tag="OQL90_002778"
     CDS             6909..7709
                     /gene="yigL"
                     /locus_tag="OQL90_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1369495.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7785..8684
                     /gene="bioP"
                     /locus_tag="OQL90_002779"
     CDS             7785..8684
                     /gene="bioP"
                     /locus_tag="OQL90_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1369496.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8572..9525)
                     /gene="metR"
                     /locus_tag="OQL90_002780"
     CDS             complement(8572..9525)
                     /gene="metR"
                     /locus_tag="OQL90_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1369497.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9761..12022
                     /gene="metE"
                     /locus_tag="OQL90_002781"
     CDS             9761..12022
                     /gene="metE"
                     /locus_tag="OQL90_002781"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1369498.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12307..12552)
                     /locus_tag="OQL90_002782"
     CDS             complement(12307..12552)
                     /locus_tag="OQL90_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1369499.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12542..>12921)
                     /locus_tag="OQL90_002783"
     CDS             complement(12542..>12921)
                     /locus_tag="OQL90_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369500.1"
                     /translation="FNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVM
                     INRLKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTAT
                     LEDYKLKGELNDLYHQDALDYDN"
BASE COUNT         3052 a         3262 c         3446 g         3161 t
ORIGIN      
        1 tttctacgct gcgtataaat cgcatccatc acaaaaattg ccagcgccac ccaaataaag
       61 gcgaaggtca ccatcttatc ggcacccggt ttttcaccat aaaacgtcac tgccaacagg
      121 aacatcagcg tcgggccaat atactggaag aagcctaacg tggagagacg caggcgtgta
      181 gcagctgcgg taaaacacaa cagcggtacg gtggtgacaa taccggcggc gatcagcagc
      241 aaattcagca acatcgggtt ttgccccata tggctggttg agctgtcggc aatagcaaac
      301 agataaatcg ccgccacggg cagcagccac atggtttcga ttaacatgcc ggtttgcgct
      361 tcaacggcaa ttttcttgcg taccagtccg tagaaggcaa aactaaacgc cagccccagc
      421 gcgatgattg gcagcgaacc aaaagtccac aactgaacca acacgccaca tatcgccaga
      481 atcaccgcca gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca
      541 atgttcacca gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc
      601 accgcccaga taaacagtag ccagttgcca ccaatcagca cggcagagac tgccagcata
      661 aaaattttct gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc
      721 atcagcacca ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg
      781 ggcacgtagt aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca
      841 agagcgagta atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa
      901 ttggtaacag cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggcg
      961 gtggcactgg caatataaag ctgttcttca ttgtgtaatt caacgcgggc gacggcgact
     1021 ttattgcctg cacgcaacag gctactggta gcagtaaaac gctcgcccct gcctgggcgc
     1081 agataatcaa cgcgaagatc aatggtcccc atccgcgata gccgctggcg tagttcatct
     1141 tcactgatgg tttcgtggcg ggttaaggta cttcccacgc acaccagacc ggcggcgaca
     1201 tccagcgccg acgcaatgac tccgccgtgc aaaatgcttt gcgcccagtt acccaccatc
     1261 atcggctgat ttttaaaggc cagctgtgcg aactcttttt cgtaacgctc cagctccatc
     1321 cctaacgcgc ggttaaatgg catgtgataa acaaacatct cacccactaa tttcagggct
     1381 tgttcagcgg tcagtacggc agacatatca tccttacact tcattggtta atgaaatgtt
     1441 gattttatgc ttctttgttg ttggtttcta ctttaggaag ggataactaa cggcaatgga
     1501 gttaagtatg taaaatagcc cgcagaaaaa cattcacctt atcaataatt cgttacggag
     1561 aacacgaccg atgcggactc tgcagggctg gttgttgccg gtgtttatgt tgcctatggc
     1621 agtatatgca caagaggcaa cggtgaaaga ggtgcatgac gcgccagcgg tgcgtggcag
     1681 tattatcgcc aatatgctgc aggagcatga caatccgttc acgctctatc cttatgacac
     1741 caactacctc atctacaccc aaaccagcga tctgaacaaa gaagcgattg ccagttacga
     1801 ctgggcggaa aatgcgcgta aggatgaagt aaagtttcag ttgagcctgg catttccact
     1861 gtggcgtggg attttaggcc cgaactcggt gttgggtgcg tcttatacgc aaaaatcctg
     1921 gtggcaactg tccaatagcg aagagtcttc accgtttcgt gaaaccaact acgaaccgca
     1981 attgttcctc ggttttgcca ccgattaccg ttttgcaggt tggacgctgc gcgatgtgga
     2041 gatggggtat aaccacgact ctaacgggcg ttccgacccg acctcccgca gctggaaccg
     2101 cctttatact cgcctgatgg cagaaaacgg taactggctg gtagaagtga agccgtggta
     2161 tgtggtgggt aatactgacg ataacccgga tatcaccaaa tatatgggtt actaccagct
     2221 taaaatcggc tatcacctcg gtgatgcggt gttgagtgcg aaaggacagt acaactggaa
     2281 taccggttac ggcggcgcgg agttaggctt aagttacccg atcaccaaac atgtgcgcct
     2341 ttatactcag gtttacagcg gctatggcga atcgctcatc gactataact tcaaccagac
     2401 ccgtgtcggt gtaggagtta tgctaaacga cttgttttga tgaacggttg agtggttggc
     2461 aaatctggaa cccagcatcc aggattaccc tctcagagac taaaagcatt gcagtttctc
     2521 gcgcaggcgc tgaaaatagc gcctgttttt atttcaggca atcggggtga atgtggcgca
     2581 ggcggaagtg ttgaatctgg agtccggagc taaacaggtt ttacaagaaa cctttggcta
     2641 ccaacagttt cgccccggcc aggaagaaat tatcgacact gtgctttccg gtcgcgattg
     2701 cctggtcgtc atgcccaccg gtggcggaaa atccctttgc tatcaaattc ctgccttatt
     2761 gctaaacggc cttaccgtgg ttgtttcacc gctgatttcg ttgatgaaag atcaggtgga
     2821 tcaactgcaa gccaacggcg tggcggcggc gtgccttaac tcgacgcaaa cccgcgagca
     2881 gcaacttgaa gtgatgacag gctgccgcac cgggcaaatt cgtctgcttt atatcgcccc
     2941 ggaacgcctg atgctggata actttcttga gcatctggcg cactggaatc cggtgttatt
     3001 agccgttgat gaagcgcact gtatctccca atggggccac gatttccgcc cggaatatgc
     3061 cgcgcttggt cagttacgcc agcgtttccc gaccctgccg tttatggcgc tgactgccac
     3121 agccgacgac accacgcgcc aggatatcgt gcgcttgctg gggctgaacg atccgctgat
     3181 tcaaatcagc agttttgacc gtccgaatat tcgctacatg ctgatggaga agttcaaacc
     3241 gctcgatcag ttgatgcgct acgtgcagga acagcgcggt aagtcaggca ttatctactg
     3301 caacagccgc gcgaaagtag aagacaccgc tgcgcgcctg caaagcaagg gaattagcgc
     3361 ggcggcctat catgctgggc tggaaaataa tgttcgcgcc gacgtgcagg aaaaatttca
     3421 gcgcgatgac ctgcaaattg tggtggcgac ggtggcgttc ggcatgggca tcaataaacc
     3481 aaacgttcgc ttcgtggtcc actttgatat tccgcgcaat atcgaatcct attatcagga
     3541 aaccggtcgc gccgggcgtg atggcctgcc cgcggaagcg atgctgtttt acgatccggc
     3601 cgatatggcg tggctgcgcc gttgcctgga agagaagccg caggggcagt tacaggatat
     3661 cgaacgtcat aaactcaatg cgatgggcgc ttttgccgaa gcgcaaactt gccgtcgtct
     3721 ggtattgctg aactattttg gcgaagggcg gcaagagccg tgcgggaact gcgatatctg
     3781 cctcgatccg ccgaaacagt acgacggttc aaccgatgct cagattgccc tttccaccat
     3841 tggtcgtgtg aatcagcggt ttgggatggg ttatgtggtg gaagtgattc gtggtgctaa
     3901 taaccagcgt atccgcgact atggtcatga caaactgaaa gtctatggca tgggccgtga
     3961 taaaagccat gaacattggg tgagcgtgat ccgccagctg attcacctcg gcctggtgac
     4021 gcaaaatatt gcccagcatt ctgccctaca actgacagag gccgcgcgcc cggtgctgcg
     4081 cggcgaatcc tctttgcaac ttgccgtgcc gcgtatcgtg gcgctcaaac cgaaagcgat
     4141 gcagaaatcg ttcggcggca actatgatcg caaactgttc gccaaattac gcaaactgcg
     4201 taaatcgatt gccgatgaaa gtaatgtccc gccgtacgtg gtgtttaacg acgcaacctt
     4261 gattgagatg gctgaacaga tgccgatcac cgccagcgaa atgctcagcg ttaacggcgt
     4321 tgggatgtgc aagctggaac gctttggtaa accgtttatg gcgctgattc gtgcgcatgt
     4381 cgatggcgac gacgaagagt agtcagcagc ataaaaaagt gccaggatga agactccgta
     4441 aacgtttccc ccgcgagtca aatgtatgtt gatgttattt ctcaccgtcg ccatggtgca
     4501 cattgtggcg cttatgagcc ccggtcccga tttctttttt gtctctcaga ccgctgtcag
     4561 tcgttcccgt aaagaagcga tgatgggcgt gctgggtatt acctgcggcg ttatggtttg
     4621 ggcagggatt gcgttgcttg gcctgcattt gattatcgaa aaaatggcct ggctgcatac
     4681 gctgattatg gttggcggtg gcctgtatct ctgctggatg ggttaccaga tgctacgcgg
     4741 tgcactgaaa aaagaggctg tatctgcgcc tgcaccacag attgagctgg cgaaaagcgg
     4801 gcgcagtttc ctgaaaggtt tactgaccaa tctcgctaat ccgaaagcga ttatctactt
     4861 tggctcggtg ttctcattgt ttgtcggtga taacgttggc actaccgcgc gctggggcat
     4921 ttttgcgctg atcattgtcg aaacactggc gtggtttacc gtcgttgcca gcctgtttgc
     4981 cctgccgcaa atgcgccgtg gttatcaacg tctggcgaag tggattgatg gttttgccgg
     5041 ggcgttattt gccggatttg gcattcattt gattatttcg cggtgatgcc agacgcgtct
     5101 tcagagtaag tcggataagg cgtttacgcc gcatccgaca ttatttttca cgcatgcctc
     5161 gccgatgcta acagcgctcc caccagcata aacaacgagc cgaaaatctt attcagcgcc
     5221 ttcatctgct ttggtccttt aatccataga gcaatccgtt gagcaagggt ggcgtaaccg
     5281 atcatcacaa taatatcgac tacaatagtg gtgacgccga gcacgatata ctgcatcagt
     5341 tgcggctgct gtggcatgat gaattgcgga aatagcgccg ccagaaacac aatacttttg
     5401 ggattggtga gattcacaaa aactgcgcgc tggaacaaat gtcgacgcga ttgagtagag
     5461 gcaagcgatt taaggtcaat tgcaccagcg gcgcgccact gctggattcc cagccaaatc
     5521 aagtaagccg cgcctgccca cttcaacact tcaaacgcaa tcactgagcg ggaaaatagc
     5581 gtccccaaac ccacgccaac cagcacaata tgaatcgcca gtccggtctg aagcccagca
     5641 atagacgcca ccgcgccgcg ataaccgtgg ttgagcgagg tggtcatagt gttgattgca
     5701 ccagagcctg gcgacaggct taaaatgatc gatgtcagca ggtaggcaaa ccaccattct
     5761 aaggtcatga tgaactcccg gtgtgtctat ttttgtgcca caatacgcta ctgtcgcagc
     5821 gttgtgtcag gcacgctaaa aaaaacgatt ttacgtggtt taagaggcag attacccgat
     5881 gtttcagcag caaaaagact gggaaacaag agaaaacgcg tttgctgctt ttaccatggg
     5941 accgctgact gatttctggc gtcagcgtga tgaagcagag tttactggtg tggatgacat
     6001 tccggtgcgc tttgtccgtt ttcgcgcaca gcaccatgac cgggtggtag tcatctgccc
     6061 ggggcgtatt gagagctacg taaaatatgc ggaactggcc tatgacctgt tccatttggg
     6121 gtttgatgtc ttaatcatcg accatcgcgg gcagggacgt tccggtcgcc tgttagccga
     6181 tccgcatctc gggcatgtta atcgctttaa tgattatgtt gatgatctgg cggcattctg
     6241 gcagcaggag gtccagcccg gtccgtggcg taaacgctat atactggcac attcgatggg
     6301 cggtgcgatc tccacattat ttctgcaacg ccatccaggt gtatgtgacg ccattgcgct
     6361 aactgcgcca atgtttggga tcgtgattcg tatgccgtca tttatggcac ggcagatcct
     6421 caactgggcc gaagcgcatc cacgtttccg tgatggctat gcaataggca ccgggcgctg
     6481 gcgcgcgttg ccgtttgcta tcaacgtact gacccacagc agacagcgat atcgacgtaa
     6541 cttacgcttc tatgctgatg acccaacgat tcgcgtcggt gggccgacct accattgggt
     6601 acgcgaaagt attctggctg gcgaacaggt gttagccggt gcgggtgatg acgccacgcc
     6661 aacgcttctc ttgcaggctg aagaggaacg cgtggtggat aaccgcatgc atgaccgttt
     6721 ttgtgaactc cgcaccgccg cgggccatcc tgtcgaagga ggacggccgt tggtaattaa
     6781 aggtgcttac catgagatcc tttttgaaaa ggacgcaatg cgctcagtcg cgctccacgc
     6841 catcgttgat tttttcaaca ggcataactc acccagcgga aaccgctcta cagaggttta
     6901 aatttcttat gtaccaggtt gttgcgtctg atttagatgg cacgttactt tctcccgacc
     6961 atacgttatc cccttacgcc aaagaaactc tgaagctgct caccgcgcgc ggcatcaact
     7021 ttgtgtttgc gaccggtcgt caccacgttg atgtggggca aattcgcgat aatctggaga
     7081 ttaagtctta catgattacc tccaatggtg cgcgcgttca cgatctggat ggtaatctga
     7141 tttttgctca taacctggat cgcgacattg ccagcgatct gtttggcgta gtcaacgaca
     7201 atccggacat cattactaac gtttatcgcg acgacgaatg gtttatgaat cgccatcgcc
     7261 cggaagagat gcgctttttt aaagaagcgg tgttcaaata tgcgctgtat gagcctggat
     7321 tactggagcc ggaaggcgtc agcaaagtgt tcttcacctg cgattcccat gaacaactgc
     7381 tgccgctgga gcaggcgatt aacgctcgtt ggggcgatcg cgtcaacgtc agtttctcta
     7441 ccttaacctg tctggaagtg atggcgggcg gcgtttcaaa aggccatgcg ctggaagcgg
     7501 tggcgaagaa actgggctac agcctgaagg attgtattgc gtttggtgac gggatgaacg
     7561 acgccgaaat gctgtcgatg gcggggaaag gctgcattat gggcagtgcg caccagcgtc
     7621 tgaaagacct tcatcccgag ctggaagtga ttggtactaa tgccgacgac gcggtgccgc
     7681 attatctgcg taaactctat ttatcgtaat cgttctttat ttggtcagtt gtcaacctga
     7741 tacttcgcta caatggatac tcgttaatca aagagttttc cattgtggcg ctacttatca
     7801 tcaccacgat tctgtgggcc ttctccttta gcttttatgg cgagtacctt gcggggcacg
     7861 tcgatagcta ttttgcggtg ctggtgcgcg ttggcctggc ggcactcgtt tttctgccgt
     7921 ttctgcgtac ccgtggcaat agcctgaaaa cagtcggcct gtatatgctg gtgggcgcga
     7981 tgcagctcgg cgtgatgtat atgctgagtt tccgcgctta tctctatctg acggtttccg
     8041 aactgctgct gttcaccgta ctgacgccgc tctacatcac gctgatttat gacatcatga
     8101 ataagcgccg tctgcgctgg ggatatgctt ttagtgcctt gttggcggtg attggtgccg
     8161 ggattattcg ctatgatcag gtcaccgacc atttctggac tggcttgttg ctggtgcaac
     8221 tctccaatat cacttttgcc atcggcatgg tgggttacaa acgcctgatg gaaactcgcc
     8281 cgatgccgca gcataacgcc tttgcgtggt tctatcttgg cgcgttcctg gtggcggtaa
     8341 ttgcatggtt cttgctggga aatgcgcaga aaatgccgca aaccacgttg caatggggca
     8401 ttctggtgtt tcttggcgtg gtggcttccg ggattggcta ctttatgtgg aactacggcg
     8461 cgacgcaggt ggacgccgga acgctgggaa ttatgaataa tatgcacgtt ccggcagggc
     8521 tgctggtaaa cctggctatc tggcaccaac agccgcactg gccaacgttg attacaggcg
     8581 cgctggtgat cctggcctca ctgtgggtgc atcgtaagtg ggtcgctccg cgctcttcac
     8641 aaacggcaga tgatcgcagg cgtgattgcg cgctgagcga ataaacgctt ccgtaactgg
     8701 ctgacgctgc tcgccatcgc gcacggcggc gtacagtcgg ctccacaagc cttcgcccag
     8761 ggttttggtc accaccagac cctggcgctc aaaactctct actacccaat gcggtagcgc
     8821 ggcaataccc atccgcgcgg caaccatctg aatcaacaat aaggtgttat cgacgctttt
     8881 cagtgacgga ctgacgcctg ccggctgaag aaaatgccgc cagacatcca gtcgactacg
     8941 ctgcaccgga taaattaata gcgtctcgct ggcgagatct tccggtgtaa tacgcgtttt
     9001 tgccgccagt ggatggtcag gtgctaacac cagacgcact tcatagtcga acatcggcga
     9061 ataatgcaga ccactgcgcg gcagaatatc ggacgtcatt accagatcca gctctccctg
     9121 ttgcaaggcg ggctgcgggt caaatgtcac gcccgattta aagtccatct cgacctgcgg
     9181 ccagttctta tggaaatttt ctaacgcggg cgtcagccac tgtatacagc tatggcactc
     9241 aatggcaatg cgcagacgcg tctgctgcgg ttcattgcag gcttgcagtg cctggctaat
     9301 ttgtggcagc acctggtttg ccagttgcaa caggatttct ccctgcggtg taaagcgaag
     9361 cggctggctt ttacgcacaa atagccggaa gccaaggcgt tgttccagat cgctaaactg
     9421 gtgagacagg gcggattgcg tttgatgcaa cgtcgccgca gcggctgcga gtgagccgca
     9481 gttccgcaac gcttgtagcg ttttcagatg ttttacttcg atcatgaaag tccttcactt
     9541 cggcatgaat aatttgcgct tgaggaatat acagtaaccg ccaattatgg atgtgtaaac
     9601 atctggacgg ctaaaatcct tcgtctttta aatttatggt gcgttggctg cgtttctcca
     9661 ccccggtcac ttacttcagt aagctcccgg ggatgaataa acttgccgcc ttccctaaat
     9721 tcaaaatcca taggatttac atataattag aggaagaaaa atgacaatat tgaatcacac
     9781 cctcggtttc cctcgcgttg gcctgcgtcg cgagctgaaa aaagcgcaag agagttattg
     9841 ggcagggaac tccacgcgtg aagaactgct ggcggtaggg cgtgaattgc gtgctcgtca
     9901 ctgggatcaa caaaagcaag cgggtatcga cctgctgccg gtgggcgatt ttgcctggta
     9961 cgatcatgta ctgaccacca gtctgctgct gggtaacgtt ccggcgcgtc atcagaacaa
    10021 agatggttcg gtagatattg acaccctgtt ccgtattggt cgtggacgtg cgccgactgg
    10081 cgaacctgcg gcggcagcgg aaatgaccaa atggtttaac accaactatc actacatggt
    10141 gccggaattc gttaaaggcc aacagttcaa actgacctgg acgcagctgc tggaggaagt
    10201 ggacgaggcg ctggcgctgg gccacaacgt taagcctgtg ctgctggggc cggttacctg
    10261 gctgtggctg gggaaagtga aaggtgaaca gtttgaccgc ctgagcctgc tgaacgacat
    10321 tctgccggtt tatcagcaag tgctggcaga actggagaaa cgcggcatcg agtgggtaca
    10381 gattgatgaa cccgcgctgg tactggaact accacaggcg tggctggacg catacaaacc
    10441 cgcttacgac gcgctccagg gacaggtgaa actgctgctg actacctatt ttgaaggcgt
    10501 tacgccaaat ctcgacacga ttactgcgct gcctgttcag ggactgcatg tggaccttgt
    10561 acatggtaaa gatggcgttg ctgaactgca caagcgcctg ccttctgact ggctgctgtc
    10621 tgcgggactg atcaatggtc gtaacgtctg gcgcgccgat cttactgaga aatatgcgca
    10681 aattaaggac attgtcggca aacgtgattt gtgggtggca tcttcctgct cgttgctgca
    10741 cagccccatc gacctgagcg tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc
    10801 cttcgcctta caaaaatgcc atgaactggc actgctgcgc gatgcgctga acagtggtga
    10861 cacggcagct ctggcagagt ggagcgcccc aattcaggcg cgtcgtcact ctactcgtgt
    10921 acataatccg gcggtagaaa agcgtctggc ggcgatcacc gcccaggaca gccagcgtgc
    10981 gaatgtctat gaagtgcgtg ctgaagccca gcgtgcgcgt tttaaactgc cagcgtggcc
    11041 gaccaccacg attggttcct tcccgcaaac cacggaaatt cgtaccctgc gtctggattt
    11101 caaaaagggc aatctcgacg ccaacaacta ccgcacgggc attgcggaac atatcaagca
    11161 ggccattgtt gagcaggaac gtttgggact ggatgtgctg gtacatggcg aggccgagcg
    11221 taatgacatg gtggaatact ttggcgagca cctcgacgga tttgtcttta cgcaaaacgg
    11281 ttgggtacag agctacggtt cccgctgcgt gaagccaccg attgttattg gtgacgttag
    11341 tcgcccggca ccgattaccg tggagtgggc gaagtatgcg caatctctga ctgataaacc
    11401 ggtgaaaggg atgctgactg gcccggtgac cattctctgc tggtcgttcc cgcgtgaaga
    11461 tgtcagccgt gaaaccatcg ccaaacaaat tgcgctggcg ctgcgtgatg aagtggcgga
    11521 cctggaagcc gcaggaattg gcatcatcca gattgatgaa ccggcgctgc gcgaaggttt
    11581 accgctgcgt cgtagcgact gggatgcata tctccagtgg ggcgtggagg ccttccgtat
    11641 caacgccgcc gtggcgaaag atgacacaca aatccacact cacatgtgtt attgcgagtt
    11701 caacgacatc atggattcca ttgcggcact ggatgcagac gtcatcacca tcgaaacctc
    11761 gcgttccgac atggagttgc tggagtcgtt tgaagagttt gattatccga atgaaatcgg
    11821 tccaggcgtc tatgacattc actcgccaaa cgtaccgagc gtagaatgga ttgaagcctt
    11881 gctgaagaaa gcggcaaaac gcattccggc agagcgcctg tgggtcaacc cggactgtgg
    11941 cctgaaaacg cgcggctggc cagaaacccg cgcggcactg gcgaacatgg tgcaggcggc
    12001 gcagaatttg cgtcggggat gatgaatatt aacgagtcac tgtaaattgc agtacgatga
    12061 aagaggcaaa actgcctgat accaggccta cataatttat gcaatttatt gaatttttga
    12121 gttttgttgg ccggacaagg cgttcacgcc gcatccggca tgaacaacgc gcactttgcc
    12181 aataatctga tggcttccat caccggaagc cattttgcta tctgcgcccg ttcagataca
    12241 gaaataaccg aatcattttt acgcaaaata taatcctgag cgatttgttc agtaatcaac
    12301 gctacgttat ttattctcac ttttcacgaa attgtatccc ttttcagtaa cgtggtagtg
    12361 aggcatagac ttatgtccgc tatcctcaat aataaccaaa ccctcgttag ctaatttgtt
    12421 aacaatgtta acaacattac cgaatcctgg aatttgcctt atggatagtg tgcgatcgag
    12481 attcatccag ttccagttta tatctttctc agccataact ttcagtacgt taatttcagt
    12541 ttcagttatc atagtcaagt gcatcctggt ggtataagtc attaagttct cccttaagtt
    12601 tataatcttc tagagtagca gtatgcgtat gattccagag tgcatagcct gcgtcaccat
    12661 caggttgtcc tgtttcccaa ccaagattac agtaacgctg atattcccga cactcatgag
    12721 tatagaagtt aagatctact tgttctgggg ggatttttcc tttctcaatg tctttcagtc
    12781 tattgatcat cacttcattt acagggtcat gaccaaatct ggctatgtgt ttttcaacaa
    12841 tagcgatacc ttccctgttg attgttacgc catctgtcgt cagattttct attggaccac
    12901 cggcaaggtc gggattaaat g
//
LOCUS       DAJVOY010000112        12829 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVOY010000112 DAJVOY010000000
VERSION     DAJVOY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.112"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            41..985
                     /gene="wecH"
                     /locus_tag="OQL90_002784"
     CDS             41..985
                     /gene="wecH"
                     /locus_tag="OQL90_002784"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1369501.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALIIHALRTRGI
                     ELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1027..1464)
                     /locus_tag="OQL90_002785"
     CDS             complement(1027..1464)
                     /locus_tag="OQL90_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCS1369502.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1510..1851)
                     /locus_tag="OQL90_002786"
     CDS             complement(1510..1851)
                     /locus_tag="OQL90_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369503.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2016..2207)
                     /locus_tag="OQL90_002787"
     CDS             complement(2016..2207)
                     /locus_tag="OQL90_002787"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369504.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(2140..3468)
                     /gene="xylB"
                     /locus_tag="OQL90_002788"
     CDS             complement(2140..3468)
                     /gene="xylB"
                     /locus_tag="OQL90_002788"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1369505.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3585..4862)
                     /gene="xylA"
                     /locus_tag="OQL90_002789"
     CDS             complement(3585..4862)
                     /gene="xylA"
                     /locus_tag="OQL90_002789"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCS1369506.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5228..6199
                     /gene="xylF"
                     /locus_tag="OQL90_002790"
     CDS             5228..6199
                     /gene="xylF"
                     /locus_tag="OQL90_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1369507.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6277..7818
                     /gene="xylG"
                     /locus_tag="OQL90_002791"
     CDS             6277..7818
                     /gene="xylG"
                     /locus_tag="OQL90_002791"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCS1369508.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7796..8976
                     /gene="xylH"
                     /locus_tag="OQL90_002792"
                     /pseudo
     CDS             7796..8976
                     /gene="xylH"
                     /locus_tag="OQL90_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
     gene            9054..10232
                     /gene="xylR"
                     /locus_tag="OQL90_002793"
     CDS             9054..10232
                     /gene="xylR"
                     /locus_tag="OQL90_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCS1369509.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNREVML
                     "
     gene            complement(10340..11164)
                     /locus_tag="OQL90_002794"
     CDS             complement(10340..11164)
                     /locus_tag="OQL90_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCS1369510.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11484..>12829
                     /locus_tag="OQL90_002795"
     CDS             11484..>12829
                     /locus_tag="OQL90_002795"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1369511.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWV"
BASE COUNT         3338 a         3164 c         3044 g         3283 t
ORIGIN      
        1 aatttactgg attgataacc tgcgagggat agcgtgttta atggtggtga tgattcacac
       61 cactacctgg tatgtgacca atgctcatag tgttagcccc gtcacatggg atatcgccaa
      121 tgttctgaat tctgcctctc gtgtcagcgt gccgctattt ttcatgattt ccggctatct
      181 cttttttggc gaacgcagcg cccagccgcg ccatttcttg cgtatcggct tatgtctgat
      241 tttttatagc gcaatcgcac tgctctacat tgcgctgttt acctccatca atatggagtt
      301 agcgctgaaa aacctgctgc aaaagccagt gttttaccac ttgtggtttt tcttcgcgat
      361 tgcggtgatt tatctggttt caccgctgat tcaggtgaag aacgtcggcg gaaaaatgtt
      421 gctggtgcta atggcggtga ttggcattat cgctaaccca aacacagtgc cgcagaaaat
      481 tgacggtttt gaatggctgc caattaactt atatatcaat ggcgatactt tttactacat
      541 tctgtatggc atgttgggcc gcgctatagg gatgatggac acacagcata aagcactgtc
      601 gtgggtgagc gccgcgctgt ttgcgacggg ggtttttatt atctctcgcg ggacattata
      661 tgaattgcag tggcgcggaa attttgccga tacctggtat ctttactgtg ggccgatggt
      721 ttttatctgc gcaatcgcgc tattgactct ggttaaaaac acgctggata cgcgtaccat
      781 tcgcggactt ggcttaatct cccgccattc attgggtata tacggattcc acgccttgat
      841 tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat tggccaatac tggatattat
      901 ttggatcttt tgcgcgacgt tggcagcgag tttgttactt tctatgctgg tacaacgaat
      961 cgacagaaac agattagtga gttaagtaaa agcccgttca cattggactg accgggctta
     1021 cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg ggtttattcc
     1081 ggcatcacga atattcttct gcaccgctac tgcaccaaac aagcttaaga agaaagccag
     1141 tccataaaaa cctttttcac tgagtaataa tgtcgcattc cacagaccta ccatcagtaa
     1201 tgcaacagag ataataaaga cagtcaggca ggtcatataa taaatggaag tggtcggtat
     1261 gccttcatac ttgtcccgaa cggtcttttg ataagacgca gcagaaaaca gtcctaacac
     1321 cagtacggca aaataatacc ctttttcatt taactgcatc tctgcattcc atagccctaa
     1381 cagataggta acgataccac caacgagagc tatccatgac acaatactaa aggccggtga
     1441 ataggttgat attttgttgt ccatcacagt attcctttca ttcctgaatg tgtaagagct
     1501 ttatgttgct cataccgagc gaaacaaacg catttgacca aacaagcaga caaaaaaggc
     1561 gatggggtag atcaccattt cataagcatt gataggtgca tttaaaacac cgataaacaa
     1621 gagaccgata gtgattaacg caactaactg gcagatatgg gtaaaaaaat tatccagttg
     1681 cccgagtttt tctgcgcgaa gatatgcata gttaccaaaa gttgctgtca ttaacacgcc
     1741 aagaaaatag ccttttccac ttagcaatgg gcatgagatc catagcccaa ccagataaac
     1801 cagcgccccg acaacaaata atagttttgc cacagttttt gaggttttca taacgatctc
     1861 catatctacc agcgatacat tacgagtaac caacgaaaga caaaactgaa aaatgccatt
     1921 aacaaatgat tttcagaata aattcatact aaatattaat tagtgagata tatagatgtg
     1981 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
     2041 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
     2101 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
     2161 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccgtacg
     2221 gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact cactacgcgc
     2281 cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca cgacatccat
     2341 gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt cattggggcc
     2401 atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt gtggtgtacg
     2461 ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag cctgttgagc
     2521 tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcgaccc aatccagaca
     2581 cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg catggcaaaa
     2641 gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa aatagacccc
     2701 cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac caactgcacc
     2761 agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc acgctttcgc
     2821 aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg gcatctggtc
     2881 acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg cgacatccag
     2941 ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca tacgcagacg
     3001 caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg gctcatgccg
     3061 ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc cggtaatcac
     3121 tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc gcccgtcgtt
     3181 ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg ctccatgcat
     3241 ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc ccagagcttt
     3301 cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc agagtggatg
     3361 cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct gctcgttgag
     3421 caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat cgatcgttcc
     3481 ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac agttagccgt
     3541 tatttgtcga acagataatg atttaccaga ttttccagtt gctcctagcg accactctga
     3601 tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag tgacatttgg
     3661 cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg cgcgatgcgt
     3721 ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag tgccatcgta
     3781 tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg acgacgtact
     3841 ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat ttcatacatc
     3901 accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc cagttgcgca
     3961 tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt ggctatttca
     4021 tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag tttaatctct
     4081 ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta atcatattga
     4141 tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctggaa gccgatttta
     4201 tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc ctgacgcaag
     4261 tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata gttttcaccg
     4321 cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca gctgaagact
     4381 tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa gcagttagcg
     4441 gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac atcaaccatt
     4501 tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac atccacatcg
     4561 tggaagcaat aaaatgacac atgtaacttg tggaaaaact caaatgcgac atctgcttta
     4621 cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa cgcccccaca
     4681 ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt aacaggcggc aaaacgcaag
     4741 tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg gaatgctaac
     4801 gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa ataggcttgc
     4861 atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta atattgggca
     4921 ctccctttca gttgctcaat tatgttattt cacactgcta ttgagataat tcacaagtgt
     4981 gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga agataaaaat
     5041 gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag aaataaacca
     5101 aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt tgctaaaaat
     5161 tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact acagaaggcc
     5221 ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc tgcttaccaa
     5281 cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc tacgtcttga
     5341 acgctggcaa aaagatcgag atatttttgt gaaaaaggct gaatctctcg gtgcgaaagt
     5401 atttgtacag tctgcaaatg gcaatggaga aacacaaatg tcgcagattg aaaacatgat
     5461 taaccggggt gtcgatgttc ttgtcattat tccatataac ggccaggtat taagtaacgt
     5521 tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta tgattaacga
     5581 tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac tgcaggcaaa
     5641 agccctggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct cgccggtaga
     5701 taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt acgttgattc
     5761 cggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg aaaacgcgct
     5821 aaccgccaat aataacaaaa ttgatgctgt agttgcctca aacgatgcca ccgcaggtgg
     5881 ggcaattcag gcattaagcg cgcaaggttt atcagggaaa gtagcaattt ccggccagga
     5941 tgcggatctc gcaggtatta aacgtattgc tgccggtacg caaactatga cggtgtataa
     6001 acctattaca ttgctggcaa atactgccgc agaaattgcc gttgaattgg gcaatggtca
     6061 ggagccaaaa gcagatacct cactgaataa tggcctgaaa gatgtcccct cccgcctcct
     6121 gacaccgatc gatgtgaata aaaacaacat caaagatacg gtaattaaag acggattcca
     6181 caaagagagc gagctgtaag cgttacgccc cggcgcggag cgggggcgtg atttctctcc
     6241 atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc cttatctact tgaaatgaag
     6301 aacattacca aaaccttcgg cagtgtgaag gcgattgata acgtcagctt gcggttgaat
     6361 gctggcgaaa tcgtctcact ctgtggggaa aatgggtctg gtaaatcaac gctgatgaaa
     6421 gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg aaattatttt tgcgggagaa
     6481 gagattcagg cgagtcacat ccgcgatacc gaacgcaaag gtatcgccat cattcaccag
     6541 gaattggccc tggtgaaaga attgaccgtg ctggaaaata tcttcctggg taacgaaata
     6601 acccacaatg gcattatgga ttatgacctg atgacgctac gctgtcagaa gctgctcgca
     6661 caggtcagtt tatccatttc acctgatacc cgcgttggcg atttagggct tgggcaacaa
     6721 caactggttg aaattgccaa ggcacttaat aaacaggtgc gcttgttaat tctcgatgaa
     6781 ccgacagcct cattaactga gcaggaaacg tcggttttac tggatattat tcgcgatcta
     6841 caacagcacg gtatcgcctg tatttatatt tcgcacaaac tcaacgaagt caaagcgatt
     6901 tccgatacga tttgcgttat tcgcgacggt cagcacattg gtacgcgtga tgctgccgga
     6961 atgagtgaag acgatattat caccatgatg gtcgggcgag agttaaccgc gctttaccct
     7021 aatgaaccac ataccaccgg agatgaaata ttacgtattg aacatctgac ggcatggcat
     7081 ccggttaatc gtcatattaa acgagttaat gatgtctcgt tttccctgaa acgtggcgaa
     7141 atactgggta ttgccggact cgttggtgcc ggacgtaccg agaccattca gtgcctgttt
     7201 ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg atggcaaaca ggtagatatt
     7261 cgtaactgtc agcaagccat cgcccagggg attgcgatgg tacccgaaga cagaaagcgc
     7321 gacggcatcg ttccggtaat ggcggttggt aaaaatatta ccctcgccgc actcaataaa
     7381 tttaccggtg gcattagcca gcttgatgac gcggcagagc aaaaatgtat tctggaatca
     7441 atccagcaac tcaaagttaa aacgtcgtcc cccgatcttg ctattggacg tttgagcggc
     7501 ggcaatcagc aaaaagcgat cctcgcccgc tgtctgttac tcaacccgcg cattctcatt
     7561 cttgatgaac ctaccagggg tattgatatt ggcgcgaaat atgagatcta caaattaatt
     7621 aaccaactcg tccagcaggg tattgccgtt attgtcatct cttccgaatt acctgaagtg
     7681 ctcggcctta gcgatcgtgt actggtgatg catgaaggga aactaaaagc caacctgata
     7741 aatcataacc tgactcagga gcaggtgatg gaagccgcat tgaggagcga acatcatgtc
     7801 gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca tccggtggct tttccgggct
     7861 gaaatcactg aatttgcagg tcttcgtgat gattgcagct atcatcgcaa tcatgctgtt
     7921 ctttacctgg accaccgatg gtgcctactt aagcgcccgt aacgtctcca acctgttacg
     7981 ccagaccgcg attaccggca tcctcgcggt aggaatggtg ttcgtcataa tttctgctga
     8041 aatcgacctt tccgtcggct caatgatggg gctattaggt ggcgtcgcgg cgatttgtga
     8101 cgtctggtta ggctggcctt tgccacttac catcattgtg acgttggttc tgggactgct
     8161 tctcggtgcg tggaacggat ggtgggtcgc gtaccgtaaa gtcccttcat ttattgtcac
     8221 cctcgcgggc atgttggcat ttcgcggcat actcattggc atcaccaacg gcacgactgt
     8281 atcccccacc agcgccgcga tgtcacaaat tgggcaaagc tatctccccg ccagtaccgg
     8341 cttcatcatt ggccgcttgg cttaatggct tttgttggtt ggcaatggcg cggaagaatg
     8401 cgccgtcagg ctttgggttt acagtctccg gcctctaccg cagtagtcgg tcgccaggct
     8461 ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggcgttccc
     8521 actcctgttc tgctgctgac gttgctgtta ctcggcggaa tgtttatggc aacgcggacg
     8581 gcatttggac gacgcattta tgccatcggc ggcaatctgg aagcagcacg tctctccggg
     8641 attaacgttg aacgcaccaa acttgccgtg ttcgcgatta acggattaat ggtagccatc
     8701 gccggattaa tccttagttc tcgacttggc gctggttcac cttctgcggg aaatatcgcc
     8761 gaactggacg ctattgcagc atgcgtgatt ggcggcacca gcctggctgg cggtgtggga
     8821 agcgttgccg gagcagtaat gggggcattt atcatggctt cactggataa cggcatgagt
     8881 atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca
     8941 gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc
     9001 cggttacgta ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta
     9061 ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag
     9121 tagaaggcgt aggggaatat ttacaggcgt cacaatcaga atgggatatt ttcattgaag
     9181 aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg
     9241 acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg
     9301 ttggcggctc gtatcacctt gcagaaagtt acccacccgt tcattacatt gccaccgata
     9361 actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg
     9421 ctttttatgg tcttccggaa tcaagcggca aacgttgggc cactgaacgc gaatatgcat
     9481 ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg
     9541 cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa acgctgccac
     9601 cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg
     9661 aacatctaca cattcccgta ccggaaaaat tatgcgtgat tggcatcgac aacgaagaac
     9721 tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg
     9781 gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc
     9841 gaattttggt cccaccagtt cgcgtcattg aacggcgctc aacagattat cgctcgctga
     9901 ccgatcccgc cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta
     9961 aagtggatca ggtactggat gcggtcggga tctcgcgctc caatcttgag aagcgtttta
    10021 aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc
    10081 gcagtctgct gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc
    10141 catcgctgca atatttctac tctgttttta aaaaagcata tgacacaacg ccaaaagagt
    10201 atcgcgatgt aaatcgtgag gtcatgttgt agcgatgatg agaattgtcg gcgtcacatc
    10261 agggaactca gcacagccac ctgatgcgag caaaagcctt atccgaccga caaatcagat
    10321 aaggctcaag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca
    10381 ttgcgaacag gtagttatta taactcttcc ctttggtcga gtagcccttc agcttgtgaa
    10441 tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc gatttacgga
    10501 acgaagagta agccgggtgc gtattcaggt tagtgacata ggcgctcacc gattctttga
    10561 cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca
    10621 tgcatttcat gccgaacaga ttgttattgt tgcgcgccag cttcgacgtt ccccaaccgc
    10681 tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca
    10741 aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga
    10801 tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca
    10861 gccagttacg ttctgcagta atggctgcat tttggctggt aatgtaaggc attacggtcc
    10921 ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac
    10981 tactctttgc actattgcga gaatactctt gtttactgct tacctgttta ttactagctt
    11041 tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg
    11101 aaaacaccag agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa
    11161 tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttc acaaatttga
    11221 gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa
    11281 atctgaaact atgtcacgag ttaacgattc agattggcgc taaatcgcag aaaatgtggg
    11341 gtttatcgca aaattcagcc gttttttgcg cgcgatcgct cacccttgct tctcatcctg
    11401 tggacttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc
    11461 gcagcaataa ggactcatcc accatgaaac tcgcctcctg ttttctgaca ctccttcctg
    11521 gcttcgccgt tgccgccagc tggacttctc cggggttccc tgcctttagc gaacagggaa
    11581 cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac gcgtccactc acgctaaatt
    11641 ttgaccaaca gtgctggcag cctgcagatg cgataaaact caatcagatg ctttccctgc
    11701 aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcaaa tatacgctgc
    11761 aaatagacac ccgctccggt acgccaacat tgatgatttc catccagaac gccgccgaac
    11821 cggtagcaaa cctggtccgt gaatgcccga aatgggatgg attaccgctc acgctggatg
    11881 tcagcgccac tttcccggaa ggagccgccg tacgggatta ttacagccag caaattgcga
    11941 tagtgaagaa cggtcaaata acgttacaac ccgctgctac cagcaacggt ttactcctgc
    12001 tggaacgggc agaaactgac acatccgccc ctttcgactg gcataacgcc acggtttact
    12061 ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt tacggacgtc
    12121 ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc ggcctgatca
    12181 acaaactgga ttacctccag cagttgggcg ttaatgcttt atggataagc gccccatttg
    12241 agcaaattca cggctgggtc ggcggcggta caaaaggcga tttcccgcat tatgcctacc
    12301 acggttatta cacacaggac tggacgaatc ttgatgccaa tatgggcaac gaagccgatc
    12361 tacggacgct ggttgatagc gcacatcagc gcggtattcg tattctcttt gatgtcgtga
    12421 tgaaccacac cggctatgcc acgctggcgg atatgcagga gtatcagttt ggcgcgttat
    12481 atctttctgg tgacgaagtg aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg
    12541 ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac aaaacaggct
    12601 gggataaatg gtggggaaaa aactggatca gaacggatat cggcgattac gacaatcctg
    12661 gattcgacga tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacta
    12721 ccgcttctgg tctgccggtg ttctataaaa acaaaacgga tactcacgct aaagtcatcg
    12781 aaggctttac acctcgcgat tacttaaccc actggttaag tcagtgggt
//
LOCUS       DAJVOY010000113        12794 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVOY010000113 DAJVOY010000000
VERSION     DAJVOY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.113"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            47..520
                     /locus_tag="OQL90_002796"
                     /pseudo
     CDS             47..520
                     /locus_tag="OQL90_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
     gene            complement(529..927)
                     /gene="vapC"
                     /locus_tag="OQL90_002797"
     CDS             complement(529..927)
                     /gene="vapC"
                     /locus_tag="OQL90_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCS1369512.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(927..1154)
                     /gene="vapB"
                     /locus_tag="OQL90_002798"
     CDS             complement(927..1154)
                     /gene="vapB"
                     /locus_tag="OQL90_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCS1369513.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1236..1655
                     /locus_tag="OQL90_002799"
     CDS             1236..1655
                     /locus_tag="OQL90_002799"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCS1369514.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTDDDGRTVRNGADR"
     gene            1642..6513
                     /gene="traI"
                     /locus_tag="OQL90_002800"
     CDS             1642..6513
                     /gene="traI"
                     /locus_tag="OQL90_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCS1369515.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYAAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQYVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVMAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6533..7279
                     /gene="traX"
                     /locus_tag="OQL90_002801"
     CDS             6533..7279
                     /gene="traX"
                     /locus_tag="OQL90_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCS1369516.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7334..7894
                     /locus_tag="OQL90_002802"
     CDS             7334..7894
                     /locus_tag="OQL90_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS1369517.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            8025..8237
                     /locus_tag="OQL90_002803"
     CDS             8025..8237
                     /locus_tag="OQL90_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS1369518.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            8481..8942
                     /locus_tag="OQL90_002804"
     CDS             8481..8942
                     /locus_tag="OQL90_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1369519.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8988..9197
                     /gene="hha"
                     /locus_tag="OQL90_002805"
     CDS             8988..9197
                     /gene="hha"
                     /locus_tag="OQL90_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1369520.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            9236..9826
                     /locus_tag="OQL90_002806"
     CDS             9236..9826
                     /locus_tag="OQL90_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1369521.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10066..10326
                     /gene="repA"
                     /locus_tag="OQL90_002807"
     CDS             10066..10326
                     /gene="repA"
                     /locus_tag="OQL90_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCS1369522.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10550..10624
                     /gene="tap"
                     /locus_tag="OQL90_002808"
     CDS             10550..10624
                     /gene="tap"
                     /locus_tag="OQL90_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCS1369523.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10617..11474
                     /gene="repA"
                     /locus_tag="OQL90_002809"
     CDS             10617..11474
                     /gene="repA"
                     /locus_tag="OQL90_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCS1369524.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(12536..>12794)
                     /locus_tag="OQL90_002810"
     CDS             complement(12536..>12794)
                     /locus_tag="OQL90_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1369525.1"
                     /translation="AAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIRAKLYLASLCGL
                     RFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         3109 a         3226 c         3787 g         2672 t
ORIGIN      
        1 ttaaaatcac catcatacac taaatcagta agttggcagc attaccaaat atcaggaacg
       61 accgaaggtc gctccggagt ttattccgcg tgacatcaac ccggaaatgg agaaccgtct
      121 gagtgccgta cttgccgcaa gggaagcaga aggtcgtcag atggccagcc tcttcgaacc
      181 ggatgtcccg gaggttgttt ccggagaaga cgtgactcag gctgaacagc cgcaacagcc
      241 ggtgtctcct gtcatcaacg ataagaagtc agattcaggt gtgaatgttc cggcaggggg
      301 gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac tgccacccgg
      361 gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc agcaggaaaa
      421 tcatccggac acctggcagc agatgcagcg tcgtgaagag gtgaacatca atgtgcaccg
      481 ggaacgcggg gaggatgttg agccgggaga tgatttctga tgaacaggtc agctccagtc
      541 ttcagttctc aggccgccca cacgttcaaa ttcccgggtg ttattagtca caataatcag
      601 cccccgactg cgggcatgac ctgcgatcat ttgatcaaat ggcccgacag ggcgtccctg
      661 acgggcaagt tctgctctta tctggccggt gtgtgtggct gcagcagcgt cgtaatccag
      721 aacatcaatg cgggaaacaa atccctcgat cacagcgaga ttacgttcag gcatctggct
      781 tttttctgca ccatatatca gctccatcag ggtgaccgaa ctgatacaca ttctcccctg
      841 gttcaggtta aaacgctccc tgacgctggc gggtttgttc tttatcgtaa aaatgcagat
      901 gttggtatcg agcataaact tcagcatcag aatgactccc tttcctgcat gccgggctgt
      961 tccctgttat ccataaaatc ggcgctgacg ctgtgtccgt cgaaccattc gtcccatgtc
     1021 tctccggctg gcgtaatgat tctggttcgt ccgacagcaa tcacttcaac gcgttttacg
     1081 ttttccggca atgcaaccgc ttttggcagt ctgaccgctt ggctgcggtt gctgagaaat
     1141 acggtggttt ccattatttg cctccttatg tggatatgtc ttgtgtatat ccattgtggg
     1201 attgataccg ggatatgtca aagggatata cgtttatgat gagtatcgcg caggtcagat
     1261 cggccggaag tgccggtaat tattatacca acaaggataa ttactatgtg ctgggcagca
     1321 tgggagaacg ctgggccggc cagggggctg aacaactggg gctgcagggc agtgtcgata
     1381 aggatgtctt tacccgcctt ctggagggca ggctgccgga cggggcggat ttaagccgca
     1441 tgcaggatgg cagtaacagg catcgtcccg gctacgacct gaccttctcc gctcccaaaa
     1501 gtgtctccat gatggccatg ctcggggggg acaaacgtct tattgaagca cataaccagg
     1561 ccgtggattt tgctgtccgt catcacccgt gtggaggcac tggcctccac acggatgatg
     1621 acggacggac agtcagaaac ggtgctgacc ggtaatctgg tgatggcact gtttaaccac
     1681 gacaccagtc gcgatcagga accacagtta cacacgcatg cggtggtgac taatgttacg
     1741 cagcataacg gcgaatggaa gacactgagc agtgacaaag tggggaaaac ggggttcatt
     1801 gagaatgtgt acgctaatca gattgccttt ggcaggctct accgggaaaa actgaaagag
     1861 caggttgagg cgctgggcta tgaaactgaa gtggtcggta agcacggtat gtgggaaatg
     1921 ccgggtgtac cggtggaggc cttttccgga cgctcacaga ctatccggga ggccgtcggg
     1981 gaagacgcct cgctgaaatc ccgggatgtg gcggcgctgg atacgcgtaa atccaaacag
     2041 cacgtcgatc ctgaagtcag aatggccgag tggatgcaga cgctgaagga aaccgggttc
     2101 gacatccggg catatcgtga cgcagcggaa caacgggcat atacccgcac gcagacaccc
     2161 ggacccgctt cacaggacgg gccggatgtg cagcaggcgg tgacacaggc cattgccgga
     2221 ttaagtgaac gcaaagtgca gtttatgtat acggacctgc tggccaggac ggtcggcata
     2281 ctgccgccgg aaaatggtgt gattgaacgg gcacgcgccg gtatcgatga ggccatcagc
     2341 cgtgagcagc ttatccccct cgatcgtgag aaggggctgt tcacgtccgg gattcatatg
     2401 ctcgatgagc tgtcagtccg ggcactcagt cgtgacatca tgaaacagaa ccgggtgacc
     2461 gtacatccgg agaaaagtgt cccccggacg gccggttaca gctatgccgt cagtgttctg
     2521 gcacaggacc gtccgtcgct ggccattgtg tccgggcagg gcggtgcagc cgggcagcgt
     2581 gagcgggtgg ctgaactggt catgatggcc cgggagcagg ggcgggaggt gcagattatc
     2641 gctgctgacc gtcgctcgca gatgaacctg aagcaggatg aacggctgtc cggtgaactg
     2701 ataaccggac gtcgtcagtt gctggaaggc atggccttca cgccgggcag taccgttatc
     2761 gtcgaccagg gcgaaaaact ctccctgaaa gagacgttaa ccctgctgga tggtgccgca
     2821 cgtcataacg tacaggtcct gataactgac agcgggcagc gaaccggtac aggcagtgca
     2881 ctgatggcca tgaaggatgc cggggtgaac acatatcgct ggcagggggg agaacagcga
     2941 ccggccacca tcatcagtga accggaccgt tatgtccgct atgcccggct ggccggagat
     3001 tttgcggcca gcgtgaaagc cggagaagag agcgtggcac aggtcagcgg agtacgggaa
     3061 caggccatac tgacacaggc cattcgcagt gagctgaaaa cacagggcgt gctcggacac
     3121 ccggaggtaa ccatgaccgc cctgtcaccg gtctggctgg acagccggag ccgttatctg
     3181 cgggatatgt accgcccggg aatggtgatg gagcagtgga acccggagac acgcagtcat
     3241 gaccgctatg tgacagaacg tgtgacggcg cagagtcaca gcctgaccct gcggaatgcg
     3301 cagggcgaaa cgcaggtggt gcgtatttct tccctggaca gcagctggtc gctgttccgg
     3361 ccggaaaaaa tgccggtggc agacggtgag cgtctgaggg tgacagggaa aattcccgga
     3421 ctccgcgtct ccggcggtga ccgcctgcag gtggcatccg tcagtgaaga tgcgatgacg
     3481 gttgttgtgc cgggacgggc tgaaccggcc accctgcctg tgagcgattc accgttcacg
     3541 gcactgaagc tggagaacgg ctgggtggaa acgcccgggc attccgtcag cgacagtgcg
     3601 acggtttttg cctccgtcac acagatggca atggacaatg ccaccctgaa cggtctggcc
     3661 cgcagtggtc gtgatgtccg gctgtattcc tcactggatg aaacccgtac tgcggaaaaa
     3721 cttgcccgcc atccttcctt tacggtggtt tctgagcaga taaaggcgcg tgccggtgag
     3781 acattgctgg aaaccgctat cagtctgcag aaagccgggc ttcacacgcc ggcacagcag
     3841 gccattcatc tggcccttcc tgtgctggaa agtaaaaacc tggccttcag catggtggac
     3901 ctgctgacag aggcgaagtc gtttgctgca gaaggaaccg gttttgctga cctgggaggg
     3961 gaaatcaatg cgcagataaa acggggtgat ttactgtatg tggatgtggc aaaaggctat
     4021 ggcacaggcc tgctggtttc ccgtgcgtcg tatgaggcag aaaagagcat tcttcgccat
     4081 attctcgaag gtaaggaggc ggtcacgccg ctgatggaga gagtacctgg cgaactcatg
     4141 gagaaactga catcaggaca gcgcgccgcc gcccgtatga tactggaaac gtccgaccgt
     4201 ttcacggtgg tgcagggcta tgccggtgtg ggtaagacca cacagttccg ggcggtgatg
     4261 tcagccgtga acatgctgcc ggagagtgaa cgtccccgtg tcgtggggct ggggcccaca
     4321 caccgtgcgg tcggggagat gcgcagcgcc ggcgtggatg cacagacact ggcgtccttt
     4381 ctgcatgaca cgcagctgca gcagcgcagc ggagaaacgc cggatttcag caacacgctg
     4441 ttccttctcg atgagagctc tatggtgggc aataccgaca tggcacgggc atacgccctg
     4501 attgcggccg gtggcggtcg ggctgtggcc agcggtgaca cggaccagct gcaggccatc
     4561 gcgcccggtc agcctttccg tctccagcag acgcgcagtg ctgccgatgt ggtcatcatg
     4621 aaggagattg tgcgtcagac gccggaactg cgggaggcgg tatacagcct gattaaccgg
     4681 gatgtggaaa gggcgctgtc cgggcttgag cgtgtgaaac cgtctcaggt gccacgtctg
     4741 gagggcgcat gggcaccgga gcactccgtg acggagttca gtcacagcca ggaagcgaaa
     4801 ctggcagaag cgcagcagaa ggcgatgctg aaaggcgagg cttttccgga tgtccccatg
     4861 acactgtatg cagccattgt ccgcgactat accggcagga caccggaagc acgggagcag
     4921 acgctgattg tcacgcacct gaatgaggac cggcgcgtac tgaacagcat gattcatgat
     4981 gcacgggaaa aggccggaga actggggcag gtgcaggtca tggtgcctgt cctgaacaca
     5041 gcgaatatac gcgacgggga gctgcgtcgt ctctccacct gggagaataa cccggatgcc
     5101 cttgccctgg tggataatgt gtatcaccgg attgccggta tcagcaagga tgacgggctg
     5161 ataaccctgc aggatgcgga aggtaacacg cggctgattt cgccccggga ggcggtggct
     5221 gaaggcgtca cgctgtacac cccggacacc atccgggtgg ggaccggtga caggatacgc
     5281 ttcacgaaga gtgaccggga gcgcggttat gtggccaaca gcgtctggac ggtgacagcg
     5341 gtttccggtg acagtgtcac actttctgac ggtcagcaga cccgtgtgat tcgccccggt
     5401 caggagcggg cagagcaaca tattgacctg gcctatgcca tcaccgccca cggtgcacag
     5461 ggggcgagtg aaacctttgc catcgcgctt gaaggcacgg aaggtaaccg gaaactgatg
     5521 gccggctttg agtcagccta cgtggccctg tcgcgtatga agcagtatgt gcaggtgtac
     5581 accgataacc gtcagggctg gacggatgcc atcaacaatg ccgtacagaa aggaactgcc
     5641 cacgatgtgt ttgagccgaa accggaccgg gaggtcatga atgcagagcg gctgttcagt
     5701 acggcgcggg agctgcggga cgtggcggca ggccgtgccg ttctccgtca ggcggggctg
     5761 gccgggggag acagtcctgc acggtttatt gctccgggac gtaaatatcc gcaaccgtat
     5821 gtggcactgc cggcgtttga ccgtaacggc aagtccgccg gtatctggct gaacccgctg
     5881 accacggatg acggaaacgg gctgcgggga ttcagcggtg aaggtcgggt aaaaggcagc
     5941 ggggatgcgc agttcgtggc cctgcagggc agccgtaacg gagaaagcct gctggctgat
     6001 aatatgcagg atggcgtccg gattgcccgt gataatcctg acagtggtgt ggtggtaaga
     6061 atcgccggtg aaggccgtcc gtggaatccc ggtgccatca ccggtggccg cgtgtggggg
     6121 gatatcccgg acaacagcgt ccagccggga gccggaaatg gcgaaccggt catggcagag
     6181 gtactggcac agcggcaggc tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat
     6241 gaaattgtcc gtaaaatggc agagaacaaa cctgacctgc cggacggcaa aacagagcag
     6301 gctgtcaggg aaattgccgg gcaggagcgt gaccgggctg ccataactga acgggaagcc
     6361 gcgctgccgg agagtgtact gcgtgaacca caacgggtgc gggaagcggt ccgggaagtt
     6421 gcccgggaaa atttgctgca ggagcgtctg cagcagatgg agcgggatat ggttcgtgac
     6481 ctgcagaaag agaaaacccc gggcggagac tgatacagga agacaaacac tgatgacaac
     6541 ggataacacg aacacgacac gtaacgattc actggctgca cggaccgata cctggttgca
     6601 gtcatttctg gtctggtcac ccggacagcg ggatatcatc aaaacggtgg cactggtgct
     6661 gatggtactg gaccacatca atctgatatt ccagctgaag caggaatgga tgtttctggc
     6721 cgggcgagga gcctttccgc tttttgccct ggtgtggggg ctgaatctgt cccgtcatgc
     6781 gcatatccgg caaccggcca ttaaccggct gtggggatgg ggaattattg cccagttcgc
     6841 atattatctg gcaggctttc cctggtatga ggggaatatc ctgtttgcct ttgcagtggc
     6901 ggcacaggtg ctgacgtggt gtgaaacgcg cagtgggtgg cgtacagcag cggccatact
     6961 cctgatggcc ctgtgggggc ctttgtccgg caccagttac ggtattgccg ggttgctgat
     7021 gctggcagtc agcaaccggc tgtaccgggc ggaagacaga gcggaacgtc tggcgctggt
     7081 cgcctgcctg ctggctgtta ttccggcgct taaccttgcc accagtgatg cagcggcggt
     7141 ggccggtctt gtgatgacgg tgctgaccgt tggtctggtg ttgtgtgccg gaaaatcatt
     7201 accccgtttc tggcccggag actttttccc gacgttctat gcctgtcatc tggctgtgct
     7261 gggcgttctg gcgctgtgac gggtgtggta tctttggccg caagaggatg attcgtcaga
     7321 ggcagaacac agcatgacag agcagaagcg accggtactg acactgaagc ggaagacaga
     7381 gggaacagcg actgtccgca gccggaaaac catcatcaat gtcaccacgc caccaaagtg
     7441 gaaggtgaaa aagcagaagc tggccgagaa agcggcccgt gaagcagagc tggcggcaaa
     7501 aaaagcgcag gccagacagg cgctgtccat ttatctgaac ctgcccacgc tggatgaggc
     7561 tgtgaatacc ctgaagccct ggtggccggg attatttgac ggtgacacgc cccggcttct
     7621 ggcctgcggt atccgggacg tgttactgga agacgtggcg cagcggaata tcccgctctc
     7681 gcataaaaaa ctgcgcaggg cgctgaaggc catcacccgt tcagaaagct atctgtgtgc
     7741 catgaaagcc ggtgcctgcc ggtatgacac ggaagggtat gtgacggagc atatttctca
     7801 ggaggaggaa gcgtatgcgg gagcgcgtct ggcgaaaatt cgtcgccaga accggataaa
     7861 ggcagaactt caggccgtgc ttgatgagaa ataaaaaaag cctcccccag agagaggagg
     7921 cagggaaata aggcttaaaa ggaaataact tccgcttccg aaaggaagcc agtccactga
     7981 attatttatc acaataatac tgacaactaa agggggacaa gaaaatgaat ggattcagaa
     8041 acagttcacg gaacggccag gtctggcgtt accagcgtgc cggcgggcgg gccgttattc
     8101 tggaagtcag tggacgctgg atggaagcgg cagaagcatg gcgacgggct gcctgtgttg
     8161 cgcctaggac agactggcag cagtttgccc ggaaaagggc tgaacactgc caccggcgct
     8221 gccggggaag ggggtaacga aaaaagcagc cttcattact ccaggagtca gaaggctgaa
     8281 aggagtttat gagatttgct tcttaatatc cggtcgggca gtatgaccgg acagttctga
     8341 gtagcacaga gtcttttccg cattaagtcg attttttata ttttgtgatt aatttcacaa
     8401 aataaggtgt tattcaatgt gtgctgcact attcaggggg gctgaaatac aggtgccatt
     8461 tatctgatat ggagaataac atgaggaaat atattccact ggtattattt atcttttcat
     8521 ggccggtatt gtctgcagat attcatggac gggttgttcg tgtactggac ggtgatacca
     8581 tcgaggtcat ggactcactg aaggcggtgc ggatcaggct ggtaaatatt gatgcaccgg
     8641 aaaagaaaca ggattatggc cggtggtcaa cagacatgat gaaatcactg gttgcaggaa
     8701 aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc gggcaggttt
     8761 atgcaccaga cggaatgaat attaatcagt ttatggtccg ggccggggct gcctgggtat
     8821 atgagcagta caacacagat cccgtgctac ctgtccttca gaacgaagcc agacaacaaa
     8881 aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggagg caccgtaaat
     8941 aaagcgatgg caatcctatc attcatctgt tctcagcggg aataaaaatg gcaaaaacaa
     9001 aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg gaaaaaatta
     9061 ttcataaaaa cagagacagt ttatcaaaca gtgaacgtga atcatttaat tcagcagcag
     9121 accaccggct agcggaatta atcaccggaa agttatatga caggatacca aaagagatat
     9181 ggaaatatgt aaggtaataa ttcagcaata attaattgcc aggagcagat aaataatgaa
     9241 gttgattatt tttattctca ttgttctgat tattgcagcg ctacttatcc ggattatcct
     9301 cagaagtgta aatcagcatt ctcctttgct gatgcaattg catgcggcag gtatacggac
     9361 cggtgatgca gagcgaatac tgtccagcgg tgaatactgg caacgtcaga cgacgctgct
     9421 gacagaaagg gaagtcagtt ttatgaaagg actgttcaga attgtggata tgacgcggtg
     9481 gtatctgtgt ccgcaggtac gggtcgcgga tatcgtccag ctgaacggga atatccggcc
     9541 acgatcacgc cagtggtggc agttattcag gatggtgtct cagtggcatg ttgatgtggt
     9601 tatcgttgag ctgcgttcgt tcagtattgt tgcagcagta gagctggatg atgccagcca
     9661 tttacgaccg gaacgcagac gccgggatat tcttctggaa gaggttctga ggcaggctgg
     9721 tattccgttg ctcagaagcc acgatgccag aaaactgctg cagatgaccg gagaatggct
     9781 gaatacaaca ggggctgatc agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc
     9841 tcagttgtcc aaccccggaa acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat
     9901 aactgaaaac catactattt cacagtttaa atcacattaa acgacagtaa tccccgttga
     9961 tttgtgcgcc aacacagatc ttcgccacaa ttctcaagtc gctgatttca aaaaactgta
    10021 gtatcctctg cgaaacgatc cctgtttgag tattgaggag gcgagatgtc gcagacagaa
    10081 aatgcagtga cttcctcatt gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact
    10141 gactctgaga aacaaatggc cgctgttgca agaaaacgtc ttacacacaa agagataaaa
    10201 gtttttgtca aaaatcctct gaaagatctc atggttgagt actgcgagag agaggggata
    10261 acacaggctc agttcgttga gaaaatcatc aaagatgaac tgcagagact ggatatacta
    10321 aagtaaagac tttactttgt ggcgtagcat gctagattac tgatcgttta aggaatttta
    10381 tggctggcca cgccataagg tggcagggaa ctggttctga tgtagatgta caggagccag
    10441 aaaagcaaaa accccgataa tcttctttaa ctttggcgag tcagaaagat taccggggcc
    10501 cacttaaacc gtatagccaa caatcaagct atgcggggag tatagttata tgcccggaaa
    10561 agttcaagac ttctttctgt gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga
    10621 ctgatcttca acaaacttat taccgccagg taaagaaccc gaatccggtg ttcacacccc
    10681 gtgaaggtgc ccggacgctt ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca
    10741 cctcccgttt tgatttcgcc attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc
    10801 gcatgccacc ggtactacgt cgccgggcca ttgatgcgct gctgcagggg ctgtgttttc
    10861 actatgaccc actggccaac cgcgtccagt gttccatcac cacgctggcc attgagtgcg
    10921 gactggcgac ggagtctgct gccggaaaac tctccatcac ccgggccacc cgggccctga
    10981 cgttccttgc agagctggga ctgattacct accagacgga atatgacccg cttatcgggt
    11041 gctacattcc gaccgatatc acgttcacat ctgcactgtt tgctgccctc gatgtatcag
    11101 aggaggcagt ggccgccgcg cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaaa
    11161 agcaggggct ggataccctg ggtatggatg aactgatggc gaaagcctgg cgttttgttc
    11221 gtgagcgttt ccgcagttac cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg
    11281 cgcgtcgtga tgcggacagg cagcgtcagg atattgtcac cctggtgaaa cggcaactga
    11341 cgcgtgaaat ctcggaaggg cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg
    11401 agcgtcgtgt gaaggagcgc atgattctgt cacgtaaccg taattacagc cggctggcca
    11461 cagcttcccc ctgaaagtga cctcctcaga ataatccggc acgcgccgga ggcattcgca
    11521 cgcctgaagc ccgccagtgc acaaaaaatc agcgctacat acaaaaaaca acctcatcat
    11581 ccaccttctg gtgcatccgg tcccccctgt ttttgataca aaacacgcct cacagacggg
    11641 gaattttgct tatccacatt aaactgcaag ggacttcacc ataaagttac aaccgttcac
    11701 gtcataaagc gccagccgtc agccctacag ggtgcaatgt atcttttaaa cacctgttta
    11761 tatctccttt aaactactta attacattca tttaaaaaga aaacctgttc actgcctgtc
    11821 ctgtggacag acagatatgc acctcccacc acaagcggcg ggccccgacc gaagccggtt
    11881 tagttacaac atgcaaacat aaacccctaa aaaacccaag tccgacgcag aactgaaacc
    11941 acaaagcccc tccctcataa ctgaaaagcg gccccgcccc ggcccgaagg gccggaacag
    12001 agtcgctttt aattatgaat gttgtaacta tattatcatc gctgtcagtc ctctggctgg
    12061 acgtatcgag tacacgctcg taagcggccc tcacggccct cacggccctc acggcccgct
    12121 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggctccgacc gcgcacagaa
    12181 gctctgtcat ggctgaaaac gggtatggct tggcagctac catgttctgt caagaaaaat
    12241 cgcttgaact ccgacgtaca gccccctaag tactgccagc aagctgtcag cactaagtcc
    12301 ccgatacagc gcggcgtagc cgcgctaacg gggttaaaag ggcttaccta tacttcatca
    12361 tcgctgaaag cccgcatggc ctcgcagggc agagatggga taggtgaaac ctatcaatac
    12421 gtaccggctt acgccgggct tcggcggtgt tactcctgta ccatatgtaa ctacatcatg
    12481 ccgccttaca tgcgctacgc gcatatgaag acaaatagcc cacatcaaac ggtttttagg
    12541 tcttcagtac gccatagcac cagtgcacga gttttctcat cagcgcacca atggcacaca
    12601 ttttggcctt acctctcagg cataatcgct catacatcgc tttcatcaat gggttaaatc
    12661 tcagaccaca caacgaagcc agatacagtt tcgcccggat ttccggcgga ccaatttttg
    12721 acagtttcgg acaccctctg accgaggtgc cggaatattt ttcaacggga acgacaccta
    12781 aaaatgcagc cgcc
//
LOCUS       DAJVOY010000114        12784 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVOY010000114 DAJVOY010000000
VERSION     DAJVOY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.114"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..455)
                     /locus_tag="OQL90_002811"
     CDS             complement(<1..455)
                     /locus_tag="OQL90_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1369526.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETG"
     gene            complement(485..1201)
                     /locus_tag="OQL90_002812"
                     /pseudo
     CDS             complement(485..1201)
                     /locus_tag="OQL90_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1510..2286
                     /gene="exuR"
                     /locus_tag="OQL90_002813"
     CDS             1510..2286
                     /gene="exuR"
                     /locus_tag="OQL90_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS1369527.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2631..3293
                     /locus_tag="OQL90_002814"
     CDS             2631..3293
                     /locus_tag="OQL90_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1369528.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3297..3680
                     /gene="mzrA"
                     /locus_tag="OQL90_002815"
     CDS             3297..3680
                     /gene="mzrA"
                     /locus_tag="OQL90_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1369529.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3827..4195
                     /locus_tag="OQL90_002816"
     CDS             3827..4195
                     /locus_tag="OQL90_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCS1369530.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4233..4538
                     /locus_tag="OQL90_002817"
     CDS             4233..4538
                     /locus_tag="OQL90_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1369531.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4541..4945
                     /locus_tag="OQL90_002818"
     CDS             4541..4945
                     /locus_tag="OQL90_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1369532.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4935..5234
                     /locus_tag="OQL90_002819"
     CDS             4935..5234
                     /locus_tag="OQL90_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1369533.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5420..5812
                     /locus_tag="OQL90_002820"
     CDS             5420..5812
                     /locus_tag="OQL90_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1369534.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5882..6785
                     /locus_tag="OQL90_002821"
                     /pseudo
     CDS             5882..6785
                     /locus_tag="OQL90_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7078..7443
                     /locus_tag="OQL90_002822"
     CDS             7078..7443
                     /locus_tag="OQL90_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1369535.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7685..8041
                     /locus_tag="OQL90_002823"
     CDS             7685..8041
                     /locus_tag="OQL90_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1369536.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8092..8984)
                     /gene="yhaJ"
                     /locus_tag="OQL90_002824"
                     /pseudo
     CDS             complement(8092..8984)
                     /gene="yhaJ"
                     /locus_tag="OQL90_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            9089..9790
                     /locus_tag="OQL90_002825"
     CDS             9089..9790
                     /locus_tag="OQL90_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1369537.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9813..9977
                     /locus_tag="OQL90_002826"
     CDS             9813..9977
                     /locus_tag="OQL90_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369538.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10111..11421)
                     /locus_tag="OQL90_002827"
     CDS             complement(10111..11421)
                     /locus_tag="OQL90_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1369539.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11449..12781)
                     /locus_tag="OQL90_002828"
                     /pseudo
     CDS             complement(11449..12781)
                     /locus_tag="OQL90_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         3284 a         3246 c         3033 g         3221 t
ORIGIN      
        1 cctgtctcca gaacaccttt ggtggtttga gcaaacatca ggtttttagc cagagaaccg
       61 ttagttaacg catcggaagc aaagattttg gttgcggtaa aagtggttgc cgcatcagct
      121 ttaatttctt caccgcccca ggttactgtc agcggaacaa tcttgctagc atccaggctt
      181 tgaaccagtt gcgccgggga aactaactgc atttttacat ctttggtaac gtcgttagac
      241 cagattttgg tcatcaactg gtaagattgc agaccctgcc ccggcagata ttgcatttct
      301 actgctttcg gcaacgcggt gttatcggtc tgtgtcatat ccagagcggc atcaacgttg
      361 gccgttacag taatatcctt ttgtactgcc aatgcggagc ctgcaacaga aaacatcgct
      421 gcgaccagaa gagattttgc aaataccttt ttcattacta ttccttcgtt tattacaatg
      481 ataattaatg gaccgccagt tcaaactcgc cattatcttt ggtgatccag ttattatatt
      541 tgacaacgat tttgtcgtag ccagatatgc tttcaagatt tctttcctga ccagggaaga
      601 tgtttttatt tacggtctgt atcttacaat tttctttgct attattttta caataagcga
      661 tcgttaaaat ctttaagcgt tgattccctg cattaactaa tttttgggca gcatttactt
      721 ctaacttagc aataggttgc tgaggtggaa cactaacaac aatcccccaa attaagctaa
      781 cagttaactg catagagaaa tcttttttat ctgtggcaac gtcatcaata ctgggaaccg
      841 cttcaaattt aacgcgttaa tttttttctt tttgctctgg ttccattgca acaaagcgca
      901 cagttttgct actaccggct ggtaaagcga atttaggtgg cataacaacc agatgatttg
      961 catcaccgga tttaatctca acttcatttt cctgaggcgt cgatggatta tcgatacgga
     1021 atactgtcgc cttgatgtac tgaacttcat tacttttaga tattacgcga acgttgcctt
     1081 catcctgact attaatggaa acagccatag gataaacagt catatttgac tgtgccaaaa
     1141 aaggagcgaa cacgaataaa agcggcgcaa gccgtgataa gagattaatt ttcgccacca
     1201 tataaatttg atcttacaat attgtttcca aaaagttggt gtattagaga tgtagattta
     1261 ttgtaagtca atataaaaaa gcatttttag ttaatattaa atttaaaggc tgggaattaa
     1321 cttgctgaaa tttagtagct ttcacaatga cgatacttat tttttaggaa tttataaata
     1381 ttcacaatat taattcaata cattaattca atacattaat tcaatcacca aatttatata
     1441 tgaccaatgg catacccatg aataaagtgg tatgataact tcgtccgcat tccactccgg
     1501 gagacgcata tggaaatcac tgaaccacgc cgtttgtatc aacaacttgc cgctgacctg
     1561 aaagagcgca ttgaacaggg cgtctatctg gtgggtgata aactgcctgc cgaacgcttt
     1621 attgccgatg aaaagaacgt cagccgaacg gtagttcgtg aagccatcat catgctggaa
     1681 gttgaaggct atgtggaggt gcgtaaaggt tcaggtattc atgtggtttc caaccagccg
     1741 cgccatcagc aggcggctga caataatatg gaattcgcca attacggtcc gtttgagttg
     1801 cttcaggctc gccagctcat cgaaagtaat attgccgagt tcgcggcaac tcaggtaacg
     1861 aaacaagaca tcatgaaact gatggccatt caggaacagg cgcgcggcga acaatgcttc
     1921 cgtgattccg agtgggattt acagttccac attcaggtag ccctggcgac gcagaactcc
     1981 gccctggcgg ccatcgttga aaaaatgtgg acccagcgta gccataaccc gtactggaaa
     2041 aaactacacg aacacattga ttcccgtacc gttgataact ggtgtgatga ccacgatcaa
     2101 attctcaagg cgttaattcg caaagatccc catgctgcta agctggcaat gtggcagcat
     2161 ctggaaaaca ccaaaatcat gttatttaac gaaaccagcg acgatttcga gttcaatgcc
     2221 gaccgctatc tattcgccga aaacccggta gtgcatctcg atacggccac cagtggcagt
     2281 aaatgaaact cctgtccgac aggcgctgcg acgcgcctgt tcgcttcgtc ggtaagcgaa
     2341 aagtataaag tgtcagcctg tgtaaatcct ctcgccaccc tcccctgcat tcagcaaaat
     2401 cagactccac ggacatgtaa ttttgataac gaacaacgtt gacctttgtt acaattagat
     2461 tcaatttgaa tttatgtttt tgaatgcttt cttatctcac gatttaacag ggaatagttc
     2521 aggctgtgtt gatgtatcaa acccgcagaa cataccaaaa cagcaataac attgcggtag
     2581 tgcatctttt aaaaccagcg tggcgttaac cgattcacca ggaataatga atggaacttt
     2641 tgacccaatt gctgcaagcc ctgtgggcgc aggattttga aaccctggcc aatccatcga
     2701 tgattggcat gttgtatttt gtcttgtttg taattttgtt ccttgaaaac ggcttgcttc
     2761 cagcggcctt tttaccgggc gacagtttac tggtattggt cggcgtgttg attgcgaaag
     2821 gcgcgatggg ttacccgcaa acgattctgc tgctgaccgt tgccgccagc ctcggctgct
     2881 gggtcagcta tattcagggg cgatggctgg gcaatacccg caccgtacaa aactggctat
     2941 ctcatttacc cgcgcattat catcaacgcg cacaccatct ttttcataaa cacggtttat
     3001 cggcgctgtt aattggtcgc tttattgcgt ttgtcagaac actgctgccg acgattgccg
     3061 ggttatcagg gctgaataac gcgcgctttc agtttttcaa ctggatgagc ggtctgctgt
     3121 gggtattgat cctgacaact ctgggttaca tgctcggcaa aacgccggta tttttaaagt
     3181 acgaggacca gctgatgtca tgcctgatgc tgctcccggt ggtgctgctg gtgtttggcc
     3241 tggcaggttc tctggtcgtg ttatggaaaa agaaatatgg aaatcggggg taagggatgc
     3301 aaatacctcg catgtcgctt cgccagctag cctggtccgg cactgtttta cttctggtcg
     3361 gcacgctgtt gctggcctgg tccgcggttc gccagcaaga gtctacgctg gcgattcgtg
     3421 ccgttcatca aggcacaacg atgccagacg gtttttcgat ctggcatcac cttgacgctc
     3481 atggcattcc tttcaaaagt atcaccccca aaaacgacac tctgttaatt acatttgact
     3541 ccagcgacca gagcgccgcc gcaaaagcgg tcctcgacag aacattgccc catggctaca
     3601 tcattgcgca gcaggacaat aacagtcagg ctatgcagtg gctgacccgg ttacgggata
     3661 actctcatcg cttcggataa cttcctgtta tccgaaacat atcactcact ttggtgattt
     3721 caccgtaact gtctatgatt aatgaggcgg tggttaacac taccgcttcg ttgtctggac
     3781 aatcgttcct ttgtaacagg tccgaatcac aatggaaggt tcaagaatga aataccgcat
     3841 cgctttagct gtttctctct ttgctcttag tgccggtagt tatgccacta ccctgtgtca
     3901 ggaaaaggag caaaatatcc ttaaggagat cagctatgcc gaaaaacacc aaaaccagaa
     3961 tcgtattgac ggtctgaata aagccctgag tgaagtccgg gccaactgtt cagatagcca
     4021 gctgcgtgcc gatcatcaca agaaaatcgc aaagcagaaa gatgaggtgg cggaacgcca
     4081 gcaagattta gccgaggcga agcaaaaagg cgatgccgat aagattgcca aacgcgaacg
     4141 gaaactggca gaagcgcagg aagagctgaa aaagctggaa gcgcgcgact actaactcac
     4201 aatagtcact acttactcac ctggagaaaa ctatgtcgaa agaacacact acggaacatc
     4261 tgcgtgctga gttgaaatcc ctttccgata cgctggaaga ggtgcttagc tcatctggcg
     4321 agaagtcgaa agaagagttg agtaagattc gtagcaaagc ggagcaggca ctgaaacaga
     4381 gccgttatcg cctgggtgaa accggtgatg ccatcgccaa acaaacccgt gtcgcggcgg
     4441 cgcgtgctga tgagtatgtg cgtgaaaatc cgtggaccgg cgtgggaatt ggcgctgcaa
     4501 tcggtgtagt gctcggcgtt ctgctgtcgc gtcgttaatt atggcggaca ctcatcacgc
     4561 gcaagggccc ggtaaaagcg ttctgggcat cgggcagcga attgtttcca tcatggttga
     4621 aatggtggaa acacgtctgc ggctggcggt ggtggagctg gaagaggaaa aagctaatct
     4681 ctttcaactt ttactgatgc tgggcctgac gatgcttttc gctgcatttg gtcttatgag
     4741 cctgatggtg ctaattattt gggcggttga tccgcaatat cgcctgaatg cgatgattgc
     4801 caccaccgtg gtgttgctgc tactggcact gattggcggt atctggacgt tacgtaaatc
     4861 gcgtaaatcc accttgctgc gccatacacg ccatgagctg gcaaacgatc ggcaactgct
     4921 cgaggaggag tcccgtgagc agtaaagtcg aacgtgaacg acgtaaggcg caactgctta
     4981 gccagatcca gcaacaacgg ctggatcttt ccgccagtcg tcgtgaatgg ctggaggcaa
     5041 caggcgctta cgatcgtcgc tggaatatgc tgctaagtct gcgctcctgg gcgctggttg
     5101 gcagtagcgt gatggcgatc tggacgattc gccatcctaa tatgctggtc cgctgggcca
     5161 gacgcggttt tggcgtatgg agcgcctggc gtctggttaa aacgaccctc aagcagcaac
     5221 agcttcgcgg ttaacttccc tctggccgga gccattccgg ccctatcact caaatttttt
     5281 gaagattttt gacagttttc cttgctaaca atcatcattc accacgttta tgattctctc
     5341 catcgacagc aacgacgcta ataccgcgcc cttgcacaaa aaaacaatca gcagcctgag
     5401 tggcgcagtg gagagtatga tgaaaaaatt agaagatgtt ggtgtactgg tagcgcgcat
     5461 tttaatgccg attctgttta ttaccgcagg ctggggaaaa atcaccgctt actctggtac
     5521 ccaacaatat atggaagcaa tgggtgtccc gggttttatg ctgccgctgg tgattctgct
     5581 cgagttcggt ggtggtctgg caatcctgtt cggtttcctg actcgcacca cagccctgtt
     5641 tactgcgggc tttacgctgc tgacggcatt tttatttcac agcaagtttg ctgaaggcgt
     5701 caactcgctg atgttcatga aaaacctgac aatttctggc ggattcctgc tgctggcaat
     5761 taccggtccg ggcgcgtata gcatcgaccg cctgctgaat aaaaagtggt aagcacgcgc
     5821 tatacttaac gataaaaaac gaggaggaag ctcctcgttt ttgctattgg aggagagaaa
     5881 aatggggcaa ctgattgacg gcgtctggca tgacacctgg ccccactggc acaggcggtt
     5941 ttatcgcaga gaaagatcgt tatcatctct atgtttcact cgcctgcccg tgggcgcacc
     6001 gcacgctgat catgcgcaaa ctcaaaggac tggaaccgtt tatttccgtt tccgtagtga
     6061 acccgctgat gctggaaaac ggctggacct ttgatgacag ttttccggga gcaaccggag
     6121 atacgctcta tcaacatgaa tttctgtatc agctttatct ccacgccgat ccacactaca
     6181 gcggacgagt tactgttccc gtgctgtggg acaaaaagaa ccacaccatt gtcagcaacg
     6241 aatcagcaga aatcatccgc atgttcaata ccgcgtttga tgcgctgggc gcaaaagcgg
     6301 gtgattacta cccaccagcc ctgcaaacga aaattgacga acttaacggc tggatttatg
     6361 acaccgttaa caacggcgtg tataaagctg gttttgccac cagccagcaa gcctacgacg
     6421 aggcggtggc gaaagtgttt gaatcgctgg cgcgactgga gcagatttta ggccagcatc
     6481 gttacctgac cggcaaccag ctaaccgaag ccgatattcg cctgtggact acgctggtgc
     6541 gttttgatcc agtgtatgtg acccacttca agtgtgataa gcaccgcatc agcgattacc
     6601 tgaatctgta tggcttcctg cgcgatatct accagatgcc ggggatcgcc gaaactgtca
     6661 atttcgatca catccgtaat cattacttcc gcagccataa gaccatcaac cctacgggga
     6721 ttatttcaat tggtccgtgg caggatctcg atgaaccgca tggacgagat gttcgcttcg
     6781 gttaaaaaaa ggcaccttac gaggtgcctc tgataattag ctaagtgcat gttgttcatg
     6841 tcggatgcgg cgtgaacgcc ttatcaaacc tacaaaacgt ttatattcaa tatgttaaaa
     6901 tgaccgcgta ggccagataa gcgtagcgca tctggcgatt ttgcgcttgt catcagtctt
     6961 agcgcacctt cacggcactt tattaattcc ttgaacgaat atttactgcc atttcattca
     7021 cgtctattct taatttgctg ctttaaaaac aagtgattga gcaaaattga ggcaaaaatg
     7081 gactggtatc tgaaagtact aaaaaattat gtcggtttcc ggggtcgtgc gcggcgcaaa
     7141 gagtactgga tgtttattct ggtcaacatc atctttacgt tcgtgctggg gctgctggat
     7201 aaaatgttag gctggcaacg cgccggtggc gaaggcatcc tgacgacaat ctacggtatt
     7261 ctggtgtttt taccgtggtg ggcggttcag ttccgccgcc tgcacgacac cgaccgctcg
     7321 gcgtggtggg cactgttgtt cttaatcccg tttatcggct ggcttattat catcgtcttt
     7381 aactgccagg cgggtacgcc aggcgaaaac cgctttggac cagatccgaa actggaacct
     7441 taaaacaaat ctgctcgccg gatgtctgac tcatccggct tttatcatca tgttgatgaa
     7501 tgaataattt actaatgttt atttaaaata tttcaatgtc gttatataaa ttacctataa
     7561 aaaacaacca tgtatttatc ttaaatatca aaacctttat aacatccgaa aaagaaagat
     7621 acgaatagca tttacacaat acccggaata gactttcgtc acttttaatt aaagggatgt
     7681 ttttatgcag tggtatttag ccgtattaaa aaattatgtt ggtttctctg gtcgcgcacg
     7741 tcgtaaagag tactggatgt ttactctgat taacgccatc gtcggcgcta ttatcaatgt
     7801 cattcaattg attttaggtt tagagtttcc attcctttct ctcatttacc tggcagcaac
     7861 aatcattccg gtcattgctc tgtgtgttcg tcgtctgcac gatactgacc gttcaggtgt
     7921 gtgggcgctt ctctatctgg taccgatcat tggttggtta gttctgtttg tatttgcctg
     7981 tttagaaggc aattctggca gcaatcgtta cggaaacgat cctaagtttg gttcaaatta
     8041 atcttagaat tggggcgata tttcgcccct ttttattaac aataataaat attattttcc
     8101 gctaaaaagt ttgggaattt cccgcagaca ccaggatttc gcttccccca tactgtcacg
     8161 acgccaggcc ataataatat cgatctcgct ggtcgattcc gggctgacga cacgcaaccg
     8221 cccttccgca atatcttttt cgaccatcgg atacggcatc gtcgccacgc caagccccgc
     8281 cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct gtttgtccag
     8341 cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc gctaccgcaa ttccacgata
     8401 tttcacgcgc gtcacttcag ataacggttc cggctcctga tgaatcgggt gatcaggcgc
     8461 ggcaacgtag acgttcatta aggtatagag cttgcgcgag ttgatctccg acgaggaacg
     8521 aaaatgcata tccggcgcga taacgatatc cgcccgcccc tgctccagtc gttcccacgc
     8581 ccccgccagc acttcggtga tgattgccag ttgggtattg gcttttgccg ccagtttgtc
     8641 gattaacggg aaaaaggcag gtgtcggtac cagcgcctcg gtcacaatgg tgagatgcgt
     8701 ttcccaaccg cgcgcgaggg cttccgcatc ggtagtcagt ttatctgctg cttccagcaa
     8761 aacgcgccct cgctccagca acatcctccc gacattggtg aatttggtgc gatggcccga
     8821 gcggtcaaac agcaccacat ccagctcttc ttccagtttt tgcatggtgt agctaagtgc
     8881 ggaaggcacg cgtcccagct catccgccgc cgccgcaaaa ctgccccgac gatcgcatcc
     8941 ataacccgta gtgcttccag cgttaatgcc ctttctttgg ccatttcgtt ctcattcagg
     9001 aaatttgaac ataccgggca gaatatctgg ctaacaatgt agcgtccagc cccttaccat
     9061 aaaaggaagt aaagagaggt caagaattat gattactacc cgaactgcca ggcagtgtgg
     9121 acaagcagac tacggatggt tgcaggcccg gtatactttt tcctttggac actacttcga
     9181 cccgaaattg ttaggctatg cctccctgcg tgtgcttaac caggaagtgc tggccccagg
     9241 tgccgccttt cagccgcgaa cctatcccaa agtcgatatt ttaaatgtga ttctggatgg
     9301 agaagcagag tatcgcgaca gcgaaggcaa tcatgttcag gccagcgccg gtgaggcgtt
     9361 gctgctctct acccagccgg gtgtcagcta cagcgaacac aatctcagca aagacaaacc
     9421 gttaacacga atgcagcttt ggctggacgc ctgcccgcag cgagagaatc cgctgattca
     9481 aaaactggcg cttaatatgg gcaagcagca attaatcgcc tcgccagagg gtacgatggg
     9541 aagcctgcaa ttacgccagc aagtgtggct gcaccatatc gtgctcgaca aaggcgaaag
     9601 tgcgaatttc cagttgcatg ggccacgcgc gtatttgcaa tcgattcacg ggaaatttca
     9661 tgcgcttacg catcatgaag agaaagcggc gctgacctgc ggtgatgggg cgtttattcg
     9721 tgacgaggct aacattacgc tggttgccga ttccccactg cgcgctttgc tgatagattt
     9781 gcctgtctag ttgtttttac agggagatga tgatgagtaa aaaatcggcc aaaaagcgcc
     9841 agccagtgaa gcccgtggtg gcgaaagaac ctgctcgcac cgccaaaaat tttggctatg
     9901 aagagatgtt gagcgagctg gaagccatcg tcgcggatgc tgaaacgcgt ttagccgagg
     9961 atgaagctac cgcgtaaaga caggttgccg gatgcggcga aaacgcctta tccggcctac
    10021 aaaatccaac aacattaacg aattaaacaa cccgtaggcc ggataaggtg cgccagcatc
    10081 gcatccggca caatactcaa aacctggcgt ttatctggcc ttgctcgcca taatctcgat
    10141 aatctgccga tccgtttgct gcatcgaatg gcttgctaac gcacacaggt tggcaatcga
    10201 ctgctcaaca tcatgcgcca cgatcccttc attgccggtc acggcggtat catccagcgc
    10261 cattaacacc gctttccacg cagccgaagc actggtcgaa accttcatcg cgcagctgtt
    10321 cgacgcacca tcgcaaatca tgccgctgac atcgccgatc atactgctga tcgccatcga
    10381 gatagtttca taacgcccat ccaccagcca tgccatcccg gcggcggccc ccattgctgc
    10441 ggtcgttgcg gcacaaagtg cagacaaacg cggtaactgg ttatggatgt aaattgcgct
    10501 caaatgagaa agcatcagcg cacgcgccag tcgttcatca tccgctccga agtgttctgc
    10561 tacaaccacc acaggcattg ttgcggtgat cccctggtta cccgagccgg agttactcat
    10621 agccggaagc gtagcgccgc ccatacgcgc atcggatgcc gcgctggtac gaatcacaat
    10681 ggatgaagag agatctttcg ccagcaaacc gcgctcgcac tgtttttcca gcgtcgcgcc
    10741 aatatgcagc ccccacttac cgctcaaacc ttcctgcgat aacgcacaat ttagcttcgc
    10801 ggaatcgaga ataaagcgga tcgccgcaaa cgggacttca ttgacgaact tcaggatctc
    10861 agccagcgtc gttctggaaa gcaccgtcag cggagactct tgctcgccct ctgccacaca
    10921 cgcctgctgg gtaaacacca cactattgtg cgtctcaata tgcacaatgt tggtatgccc
    10981 gccgacgatg gtgacacacg cccacttctc accgttccag actttggcgc gtgagaagag
    11041 gatttcattg caaggttcct ggatcttaac ggagactttc cccgccgcca gcagtgcttt
    11101 ggcatcggca attgcctgcg cagttgcgtc tttcagcact tccagcccgg cgttggcatt
    11161 tccacctaac gcccccagcg ccgccgcaat cggcagcccc accattcccg tgccgggaac
    11221 ggtgacgccc agaccgttct tcatcagatt tggcgaaacc caggcttcta cacgttcaac
    11281 cggaccttcc agttctgccg cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt
    11341 acatcccagc gccggtttta cttcctcctg aacggcgagg atgtaacgct gccataacgg
    11401 atttaaagtc gaatcaaaca tattaaaaac cttaaaattt caggtaaatc aagaaaatgc
    11461 caggaacgga gaaacacaaa gcaacaaacc agtgacgata atcaggtaca gagacatccc
    11521 tttgtatttg tgcaatgccg gtactttgta aaccagccac gccgggatca ggcaccctac
    11581 catgccgaaa atcgggctac agatagaggt gaagctcaac accggtgcgt tcagtacgat
    11641 ggcgctccag gccagcaaaa tggcgaaaat catgatgccg cgctgaacaa gattttcgtt
    11701 aatcttctcg gcaggcatct tgcgacgcag gatgttcatt acgatccctt gcgttgcttc
    11761 gcgaaagcct aaatagacgc caaagaatgc agtcatgaca gcaaagatat tgagaatgac
    11821 gctgaccact ttcacccacg ctgcgccgtc accgctaata aactgtgcgg caatcgccag
    11881 cgcggaaata ttctgctcgt aggcttttac cgcttcgtca tgtcccatcg ccagcgtgaa
    11941 cgacacggcg tagaaaaaga cggtgacaaa taaaatgcca aacgcgatat tcattgcccg
    12001 caatgcttta tgccgcgcca cttcaattga tttttcccgc gagcgataag agatcaccat
    12061 cgggcttaac gtctggataa acagaatcga cgtcagggta aacggcagcg taataatggc
    12121 gtttttcacc agcagcccca gcggcggtag cgaaccgacg ttatacagat gccacattcc
    12181 caccatcgac acgcccagcg cagcgactac cagcagcttg gtcagcacca tgccggtcga
    12241 aattttgaat aacaatttct cgccgcgtga agagatcgcc accagaatgc aaatcagtac
    12301 cagaccataa aagggactgt ctgacagcac cccttccgtc acgccgaagg tatgcagata
    12361 ggaagcacta tcgttggtga tggcggtgga atagacgaac atccaaatca ccagcattac
    12421 gaaatagagc gcacctaaca ggatgcccca gtttttacct aaataaccgc taatgacact
    12481 cgggtaatct ttacattctg tgtgattctg ccagcgtatt aataaacaac cgctgaaaca
    12541 gatacattgc cgggtaacca atcaccgatg agagcaaaaa tacccacaat cccatcaaac
    12601 cgacctgcac cgggagaaaa acaatccccg cgccaatcgc catcccgata ctcataatca
    12661 cccagccggt gtcagtactg tcgaatttga tcgcttctcg ccactcgctc tcactcattc
    12721 cagcacgacc cgccggggtc gaagcgtctg caatgacgcc tttattcgat gcaatttcca
    12781 taat
//
LOCUS       DAJVOY010000115        12638 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVOY010000115 DAJVOY010000000
VERSION     DAJVOY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.115"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..314)
                     /gene="chbB"
                     /locus_tag="OQL90_002829"
     CDS             complement(<1..314)
                     /gene="chbB"
                     /locus_tag="OQL90_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1369540.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAA"
     gene            complement(612..950)
                     /gene="osmE"
                     /locus_tag="OQL90_002830"
     CDS             complement(612..950)
                     /gene="osmE"
                     /locus_tag="OQL90_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1369541.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1152..1979
                     /gene="nadE"
                     /locus_tag="OQL90_002831"
     CDS             1152..1979
                     /gene="nadE"
                     /locus_tag="OQL90_002831"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1369542.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2209..3096
                     /gene="cho"
                     /locus_tag="OQL90_002832"
     CDS             2209..3096
                     /gene="cho"
                     /locus_tag="OQL90_002832"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1369543.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3056..3631)
                     /gene="ves"
                     /locus_tag="OQL90_002833"
     CDS             complement(3056..3631)
                     /gene="ves"
                     /locus_tag="OQL90_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1369544.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3834..4304)
                     /gene="spy"
                     /locus_tag="OQL90_002834"
     CDS             complement(3834..4304)
                     /gene="spy"
                     /locus_tag="OQL90_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1369545.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4634..5602)
                     /gene="astE"
                     /locus_tag="OQL90_002835"
     CDS             complement(4634..5602)
                     /gene="astE"
                     /locus_tag="OQL90_002835"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1369546.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5595..6938)
                     /gene="astB"
                     /locus_tag="OQL90_002836"
     CDS             complement(5595..6938)
                     /gene="astB"
                     /locus_tag="OQL90_002836"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCS1369547.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6935..8413)
                     /gene="astD"
                     /locus_tag="OQL90_002837"
     CDS             complement(6935..8413)
                     /gene="astD"
                     /locus_tag="OQL90_002837"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCS1369548.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8410..9444)
                     /gene="astA"
                     /locus_tag="OQL90_002838"
     CDS             complement(8410..9444)
                     /gene="astA"
                     /locus_tag="OQL90_002838"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1369549.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9441..10661)
                     /gene="astC"
                     /locus_tag="OQL90_002839"
     CDS             complement(9441..10661)
                     /gene="astC"
                     /locus_tag="OQL90_002839"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCS1369550.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11107..11913
                     /gene="xthA"
                     /locus_tag="OQL90_002840"
     CDS             11107..11913
                     /gene="xthA"
                     /locus_tag="OQL90_002840"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1369551.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12080..>12638
                     /locus_tag="OQL90_002841"
     CDS             12080..>12638
                     /locus_tag="OQL90_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1369552.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANE"
BASE COUNT         3069 a         3423 c         3096 g         3050 t
ORIGIN      
        1 gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc atcgactttg
       61 ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg ctggatttcg
      121 ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg acctttttca
      181 ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt ttctgcctgt
      241 gcgcgcattt ttgataccag taaagaggta gacatgcccg cagaacaaaa cagataaatg
      301 tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga cactccgcaa
      361 gccttaacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt tttttgtggc
      421 tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta taaattgtct
      481 ctccttgacc tgcggttatg accctcttca catttcgggc aaataattcg cgaagataat
      541 taagaactca ggccaaaaaa accggcgcaa tggccggttt ccgttgttac tcaagctttc
      601 aaacgaagtg attacttcgc agcctgtgga tcagtgtcgt attcagcaca ggtctgataa
      661 ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc tgctttacca
      721 tcacgttgac ccaggatgta ggtctggcaa gtaccacgag catggatcat gctcacttca
      781 gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt tttgacgtct
      841 ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagccgtaca acccgccagc
      901 atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat attccgtcct
      961 cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc aagcccgtga
     1021 acgaaacggc tccgctttca gaggattcct gtatgacgtt ttatccacca ttcaacccgc
     1081 tgtcgcttgt cgtttcagta gcgacgggtt agctttaagg aagttttgtc ttttctgtct
     1141 ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca aaaccgcaga
     1201 ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac ctgcaaactt
     1261 atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc acgcttgccg
     1321 gtaagctgtg ccagatggcg attaatgagc tgcgccagga gaccggcaac gaatcactgc
     1381 aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat tgccaggatg
     1441 ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc gcggtattgg
     1501 ccagcgaaca ggcgctgcgc gaagcgggca ttgaactgag cgattttgtc cgtggcaatg
     1561 aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc agcggtgtcg
     1621 tggtgggcac cgatcatgca gcagaagcta ttaccggatt cttcactaaa tatggtgacg
     1681 gcggtacgga cattaatccg ctgtatcgtc tcaacaaacg tcagggtaaa cagttactgg
     1741 cggcattagc ttgcccggaa cacctttata agaaagcgcc aacggccgat ctggaagatg
     1801 atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat atcgacgact
     1861 atctggaagg gaaaaacgta cctcaacagg tcgccagaac aatagagaac tggtatctga
     1921 aaaccgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg aaaaagtaat
     1981 aatttgcaca tattggattg tgcgaaaaag agtaatttgt tcacgccgga tgcggcgtga
     2041 acgccttatt cgacctataa aactatgcaa attcaataca ttgcaggagt cgaataggcc
     2101 tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc gacccgcctc ttttttaatc
     2161 gcttcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt ggtacggcgt
     2221 ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc cgaacattta
     2281 cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca tggtgaaagt
     2341 gacaccatgc cgctctatat cggcaaaagc gttaacatcc gcagccgcgt cctttctcat
     2401 ttacgtaccc cggatgaagc cgccatgcta cggcaatccc gacggatcag ctggatatgt
     2461 accgccggtg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga acaacagccg
     2521 ctgtttaata aacggttgcg ccgcaatcgt cagctctgtg ccctgcaatt aaatgaaaag
     2581 cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa cctgtttggc
     2641 ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga ccatcgctga tgaacaaaaa
     2701 ctttgttatg gcctgctggg actggaaccg ttaagtcgcg gtcgtgcatg ttttcgttca
     2761 gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga acatgcgcta
     2821 cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca aggggcggtg
     2881 gcgctgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa ctggctgtgg
     2941 ctgggggcgg ttaattcgct ggaagacgcg acaacgttaa ttcggacacc cgccgggttt
     3001 gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta tgaaattact
     3061 gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac agtttacctt
     3121 gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc tggtcggtag
     3181 tgagcaattt atcgcccagt tgccacgccc cgttaatcac aaacaccacc ccgccgcgtg
     3241 agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag acatccaggc
     3301 gtgtcatgat gttgaaatcc atcgacatct gcccttccgt cagtttcgct tttaccacct
     3361 ggtccgctgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag cggtctgcgc
     3421 tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg ccgggaaaca
     3481 aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa aaatcacgtt
     3541 ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc cacagattca
     3601 ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc ttttcattgt
     3661 tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt tagtggtgat
     3721 caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct gtccaccgca
     3781 ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa agattattca
     3841 gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt ctcaaaatta
     3901 gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt ctgggtttcc
     3961 atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg cgcttcagct
     4021 tttgctttat cgaaggtatc gctgacaatg atgtcatgca ttgcgcggcg ttcttccagc
     4081 ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgcggatctg ctgtttctgc
     4141 gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa cttgcctttg
     4201 tggtgcatca tcggcttcgc gtcagccggt gctgcggcat gggccaggtt agccgcgcca
     4261 agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta tatccttcct
     4321 ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacaagat taactttact
     4381 gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa aaacactttt
     4441 gcgccaatta tggagaaaaa aagaaaattt gatggagagt gatgagagaa tattacaaca
     4501 caatgatttt gcagagatta tgaagaacta tactggatga ctggtgataa ataaagcaaa
     4561 taaccaggat taatctgtat taatttataa gaaagcaact taatacccgc agaatgattt
     4621 ctgcgggtaa gtattagctt attttttcga gcattaatcc cgcgcgtaat cccaacgcta
     4681 ccaacggatt agggaataac acatactcta catcatgggt tacggtaaaa cgttcctctc
     4741 cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa attcagcgtg tcacttgcca
     4801 tatgcatttc gaaggacggc gagtgacgag taatttgcga aaccacccga taacggagcg
     4861 gcggtgttct cacgataccg acactctcac cagatagcag cgcagcaatt gcgctggcag
     4921 ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa cgctttgcca agttccagcg
     4981 tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca ccaggttcct
     5041 gatggaacac cagcgcctcc agccccgccg cacccagcca cgtcagaaat ttctcgtccc
     5101 agggaatgtc gcgttgcggt aatacaccga actgcggatg caaggagcca cgaattgcgg
     5161 tatgtagatc aaggtgccag cgcacagatt ctttgccctg gtcataaaaa tcttccaggc
     5221 actgttccag ttcgcgcgcc cgacaggttt ctccgctttc agcaaatagc tgccaacgac
     5281 cgccaaacat tcgattcata tcgctatggc aataacgttt cccttgcttc agcgcaggag
     5341 gattcccgag gatcaccagc aaccgccaac gtaacgggat ctcgccgtga gatatcgcgc
     5401 caagcaacgc gtccagcatt tccacaggtg ccgtctcatt accgtggatc cccgcagaaa
     5461 tcaccagcgc gccttgcggt ggcgttaatg gcgtcagttc cagcacacca tcgcccagcc
     5521 agcgccagcg aacgccgttg atttctcgct cggtgataac cggtttttta cccgttaagg
     5581 tcagagcaag aaaattatcc attgccccct ccctcgcgct ggaacggata aaccgaaccg
     5641 agattcagta attggctcaa tacatccagt gcttcccgcc cttcgcgcag caattgcggg
     5701 tcggccagat cggcagcagt aagacgatcg cggtagtaac gatccaccca gtcattgagc
     5761 gcattaaaca aggtatcgtt catcatcacc gccggattca ccgcccggcg ttcttcttct
     5821 gtcaatacca cccgcaaccg caggcacgcc gggccgccgc cattcgccat gctttcacgt
     5881 aagtcaaaga cttttagttc gctaatcggg ttgtcagcgg caaggagttc attgagataa
     5941 ccccatactc cggcgtgttc ccgacactcc tgaggcagca ccaacatcat ggaaccatca
     6001 tcgcggctca acagttggct gttaaacaga taggtagaca ccgcatccga cacggaaacc
     6061 tgagttgccg gaacttctat cgccataaaa ccgttcaccc gcgcccgcag gtttgccagt
     6121 aactgtgact ggcgagcgaa cgcctgttgg tggcaaaaca gcacctggcg gttacttacg
     6181 gcaatcacgt cattatgaaa aacaccctgg tcgataacgt ccgggttttg ctgggcgaaa
     6241 atcacctgtt ggggattcac ctgattaagc cttgccaccg cctcgctggc ttcgcgagtc
     6301 tgtcgcgccg gataacggga aggccgggta tcattgcctt cttctcgccc gtagacaaaa
     6361 agttgcatac ccggctcacc gtaatgaccg ccgagacgat tgtggtttgc cgccccctca
     6421 tcaccgagca acgctacctg cggcaacgcc gaatggacgc taaatttctc ttcgtcgtta
     6481 aaaatagctt ttaacagcga ttcagtgacg ggcgcttcca gcgaacggtg aaatttattg
     6541 ttcaggttgg caacggtgag atgcactttg ccatccagcg tatcggcaga tggcgcgatc
     6601 gttgccgcat tggctaccca cattggcgaa gcggagctga cgctggaaag ccagtgcggt
     6661 gcctggcgtg caactttttc cagtacctgc tcatcgctac cactgaatcc caactgacgc
     6721 agcaccggaa taaacggacg ctcgtgcggc gggatcacgg cctgggggaa tcctgcatcg
     6781 gcaagggttt tcattttcag taagccctgt ttcgccgcca gtcgcgggtt agacacctga
     6841 aaacggtgac gggtagaggc ttcattacca aacgacaggc cagcgtaatg atgcgtcagc
     6901 cctaccagcc cgtcgaaatt aacttcccag gcgttcatcg caccacctca tcggaaaaat
     6961 ccagcccggg gttaagcgtt gcgggcaaag ttaacgagtc cgactccagg ctcgccatag
     7021 gccatgcgca gtaatctgcg gcataccagg cgctggggcg atggttaccg gatgcaccaa
     7081 tgccgccgaa tggcgcggta ctggcagcac cagtaagcgg tttgttccag ttaacaatcc
     7141 ccgcccgcgc ctccagcaac agttgatcga atttttcccg ttcgggggaa accagaccgc
     7201 aagagagtcc gaagcgagtg ttattcgcca ttagaatcgc ttcctcgaaa gaatcataac
     7261 gccagacgcg caataacggt ccgaacacct cttcatctgg tacgccagca acgcctgtca
     7321 tttcaatgat ccccggcgtc agcaacgatg tctctaattg taataagcgc ggcgcaagca
     7381 gggttcgtcc gcccatcgct tccagttgct gccaggcagt aaccacctgc tgtgcggcct
     7441 gttcagaaat cagcccgcca ataaacggct gcggttcgtc atcccagttg cccggcgtta
     7501 atcgctggct gacggcaacc agacgagcaa gaaacgcatc gccctgcgct ccgcttttga
     7561 gcaataaacg gcgggcgcag gtgcagcgtt gcccggctgt gacaaacgcc gactgaatgg
     7621 tcagatggac agccgcgtcg atatccgcca cctcatcgat aattagcgga ttattaccgc
     7681 ccatctcaag ggcgagaatt ttctccggct gaccggagag ctggcgatgc agctggtagc
     7741 cggtattggc gcgaccggta aacagcaaac cgtcgagatc ctccagcgca ctcagcgcct
     7801 gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc cggcggcaag ccagcctgct
     7861 gccataaacg cattaccgct tcgccactcc acggtgtcag ttcgctgggt ttaaagataa
     7921 tggtgttacc tgccagcaat gccggaacga tatgtccgtt cggcaaatga ccagggaaat
     7981 tatacggccc aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc gccgcaccgt
     8041 ccggcatttc actacgctgc tcgccggtac gaacgtgata cgccttaatt gatatcgcga
     8101 ttttattgat catcgccgtc acttcggttg ccgcttccca gcgcagctta cccgtttctc
     8161 tggcaataat cgcggttaat tcggctttat tgctttccag caatccggca aagcgttcga
     8221 caacgacctg acgttcagcc aatgagagcc gcgcccagcg cggaaacgcc gcacgggctg
     8281 cccgacaagc ctgttcgacc tgagcggcat cggcatcatt gccttgccat aacacctcgc
     8341 ccgataccgg attacgcttc acacgcgatg cgccctggcc cgttatccag tcaccgttaa
     8401 tccataaagt catgctgttt tctccactgc gcacaggcgc accagacgaa cgcgatcccc
     8461 tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct cggttgccgg
     8521 atcggtacgc gccagcacca cgcggaaatg gtgataattt tcattggcga ccaggcaggc
     8581 cggaaaatcg ccctgcgcag gctgcccttc tgccacttcc accagccgac ttttacggat
     8641 ggcgcgcacg cggtcgatgt cacactcaag cgttggcccg ccgtcgaaga tgtcgatata
     8701 gttgcggtaa cgaaaacctt ctttctccag caccgcgcgg gcaggcgcgg tttgcggatg
     8761 tacctgaccg atgacgtcct gggcttcctg ggataaaaag tgggtataga tcggatgttt
     8821 cggcatcagt tctgcaataa atgccttttg cccggtgccg cagagaaaat cggcgcggct
     8881 aaaatccatc gaaaagaagc gtttaccgag gctttgccag aacggtgaat agccgtgttc
     8941 gtcaatcacc ccgcgcattt cagcaaccac tttgtcatta aacttgtcgc gaaaagccgc
     9001 cataaacata aagcgcgatt tcgacagcaa atagccgttg ccctctttgc gccagtccgg
     9061 gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga tcgttactaa gaaacagcgt
     9121 cggcaatgcg ttatagacat tcagctcttt tgaggcgtga accaacgtgc cgacccgata
     9181 gttgtgccag ggatcgttca gcccaaccgc cacctcaatg gcgcaaatcc ccgccacggt
     9241 gcctgtctcg ctatcttcca gcacgaacac atagccctgc tcgcttttgg gcagttcgcc
     9301 ttgccaggtt ttgattgccc tttcgatacg cgcggaaagc gtggcttcat tagcgggaag
     9361 cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc agcgccgaga catctgagcg
     9421 ctcaacggga cggatgacca tcatgatgaa cctcggctaa caaaatgttc gcaagcagct
     9481 gcaaagcgat ccagtccggt cgtcacctct tcttcgctga cattgagcgc aggcgcaaaa
     9541 cgcaccacgt tgccacccgc aatcagtacc ataacgcctg ctttcgccgc ctcctgagag
     9601 atctgtttcg cttgtccggc gtaatcggca ttcagtacac agccaatcaa caaacctaag
     9661 ccgcgaactt cactgaacaa accgtagcgg tgattaatgg tattaagacg ctcaacaaac
     9721 cagtcgtgac gctgtttaac gccattaagc atctctggtg tgttgatgag ctccagcact
     9781 ttgcccgcca ccgccgaggc cagcggattg ccgccatagg tggtgccatg agtgccaacg
     9841 gtcatcacgc tggcgcactc ttcggttgtt aacaacgcac cgaccgggaa atcgccgccc
     9901 agcgctttgg cggtagttaa cagatcaggt gtcacgccgt agtgcatata ggcatacagt
     9961 tccccggtgc gcccgacgcc ggtttgtact tcatcaaaaa tcaacagcgc attgtggcgg
    10021 tcacacaatt cacgcagacc ttgtaaaaac gcgttgctgg ctggcaccac accgccttcc
    10081 ccctggatgg gttcgacaat caccgcacag gtggcgtcgt caatcagcgc gctggcagag
    10141 ttaatatcgt tatatgcagc atgacggata tctggtggca gtggcgcaaa atcctgtgaa
    10201 taggctggct gcccacccgc actgacagta aacagcgtgc gaccatgaaa cgcattttta
    10261 aacgccatga taccgctctt atggctgccg tagcggtcgt gagcgaattt acgcgccagt
    10321 tttagcgccg cttcgttggc ttccgcaccg gagttacaaa agaagacgcg atcggcaaac
    10381 gtggcgtcga tcaatttttt cgccagtcgc agtaccggct cgttggtgta accgttgccg
    10441 gtatgccaga acttactcgc ctgttcgttc agcgcttcac gcagttccgg atgcgcatgg
    10501 cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat actctttccc ctgctgatcc
    10561 cacaagcgcg aaccttcgcc acgtaccggt ataaagggtg ccggagcgta aacaggtatc
    10621 atccattcat caaagttttc acgcgtaatt ggctgagaca tagcgacctc tacagtaaat
    10681 aattcgttat ttatatgtta acaataagta atgtttgcgc tgtaaatgta gattgcaggg
    10741 ttcgtgccag ccagtgataa aagtgcataa acggcggagg ctaactggaa atcaaggagt
    10801 tataaccaaa ccatatgcat ttaaagtgca tataaagtga atacgtttgc gatgtgggtg
    10861 aataaaaaga ataaaaaacg caatgttatg cagaagtaaa atataattct ggaattgtga
    10921 tcattgacga aatttactgg aaattactgc gccattctga cgcagcgcgc accaaaagcg
    10981 ggcatttttt gcgccatcgt tgacatcatt aacaaccatc gatcaaatca cttaacaaca
    11041 ggcggtaagc aacgcgaaat tctgctacca tccacgcact ctttatctga ataaatggca
    11101 gcgactatga aatttgtctc ttttaatatc aacggcctgc gcgccagacc tcaccagctt
    11161 gaagccatcg tcgaaaagca ccaaccggat gtgattggcc tgcaggagac aaaagttcat
    11221 gacgatatgt ttccgctcga agaggtggcg aagctcggct acaacgtgtt ttatcacggg
    11281 cagaaaggcc attatggcgt ggcgctgctg accaaagaga cgccgattgc cgtgcgtcgc
    11341 ggctttcccg gtgacgacga agaggcgcag cggcggatta ttatggcgga aatcccctca
    11401 ctgctgggta atgtcaccgt gatcaacggt tacttcccgc agggtgaaag ccgcgaccat
    11461 ccgataaaat tcccggcaaa agcgcagttt tatcagaatc tgcaaaacta cctggaaacc
    11521 gaactcaaac gtgataatcc ggtactgatt atgggcgata tgaatatcag ccctacagat
    11581 ctggatatcg gcattggcga agaaaaccgt aagcgctggc tgcgtaccgg taaatgctct
    11641 ttcctgccgg aagagcgcga atggatggac aggctgatga gctgggggtt ggtcgatacc
    11701 ttccgccatg ctaatccgca aacagcagat cgtttctcat ggtttgatta ccgctcaaaa
    11761 ggttttgacg ataaccgtgg tctgcgcatc gacctgctgc tcgccagcca accgctggca
    11821 gaatgttgcg tagaaaccgg catcgactat gaaattcgca gcatggaaaa accgtccgat
    11881 cacgcccccg tctgggcgac cttccgccgc taatttagca gctctcctgg ctcaaactgg
    11941 gtcaggagaa ttaaccttga gaaaaatcaa caaactgtca gtaatgattt gttgcctgcc
    12001 gtcctttgtt ataccgtcac tgcgttttta gttgtctgac cacttctcta ttatcaagtt
    12061 tgatatagga aactccacga tgaatgctga gcgtaaattt cttttcgcct gtcttatttt
    12121 tgcgctggtc atttacgcta tccacgcttt cggtttattc gatctgctca ccgatttacc
    12181 ccacttacag acactcattc gccagagcgg attgttcggt tatagcctct atattctgtt
    12241 attcatcatt gccaccctct ttctgttacc aggaagcata ctggtgatcg ctggaggaat
    12301 agtttttggc ccgctcttag ggacactact ctcattaatt gccgccacgc tggcctcctc
    12361 gtgctcattc ctgctggcgc ggtggctggg acgtgattta ctgctgaaat acgtgggcca
    12421 tagccatacc ttccaggcca ttgaaaaagg cattgcgcgt aacggtattg attttcttat
    12481 tctgacccgc ttaatcccgt tgtttcctta caatattcaa aattacgctt acggattaac
    12541 caccatcgcc ttctggcctt atacccttat ttcggcactc acgaccctgc ccggtattgt
    12601 tatttatacc gtgatggcaa gcgatctcgc caatgaag
//
LOCUS       DAJVOY010000116        12571 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVOY010000116 DAJVOY010000000
VERSION     DAJVOY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.116"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            211..1308
                     /gene="mltA"
                     /locus_tag="OQL90_002842"
     CDS             211..1308
                     /gene="mltA"
                     /locus_tag="OQL90_002842"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1369553.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1385..2191
                     /gene="tcdA"
                     /locus_tag="OQL90_002843"
     CDS             1385..2191
                     /gene="tcdA"
                     /locus_tag="OQL90_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1369554.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2242..2685)
                     /gene="csdE"
                     /locus_tag="OQL90_002844"
     CDS             complement(2242..2685)
                     /gene="csdE"
                     /locus_tag="OQL90_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1369555.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2685..3890)
                     /gene="csdA"
                     /locus_tag="OQL90_002845"
     CDS             complement(2685..3890)
                     /gene="csdA"
                     /locus_tag="OQL90_002845"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1369556.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4082..4309
                     /locus_tag="OQL90_002846"
     CDS             4082..4309
                     /locus_tag="OQL90_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1369557.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4660..5577
                     /gene="gcvA"
                     /locus_tag="OQL90_002847"
     CDS             4660..5577
                     /gene="gcvA"
                     /locus_tag="OQL90_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1369558.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5596..5991
                     /locus_tag="OQL90_002848"
     CDS             5596..5991
                     /locus_tag="OQL90_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1369559.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5984..7084
                     /gene="rlmM"
                     /locus_tag="OQL90_002849"
     CDS             5984..7084
                     /gene="rlmM"
                     /locus_tag="OQL90_002849"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1369560.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7128..7859)
                     /gene="fucR"
                     /locus_tag="OQL90_002850"
     CDS             complement(7128..7859)
                     /gene="fucR"
                     /locus_tag="OQL90_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1369561.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7917..8339)
                     /gene="fucU"
                     /locus_tag="OQL90_002851"
     CDS             complement(7917..8339)
                     /gene="fucU"
                     /locus_tag="OQL90_002851"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1369562.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8341..9759)
                     /gene="fucK"
                     /locus_tag="OQL90_002852"
     CDS             complement(8341..9759)
                     /gene="fucK"
                     /locus_tag="OQL90_002852"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCS1369563.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILIVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9756..11501)
                     /gene="fucI"
                     /locus_tag="OQL90_002853"
     CDS             complement(9756..11501)
                     /gene="fucI"
                     /locus_tag="OQL90_002853"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCS1369564.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(11534..>12571)
                     /gene="fucP"
                     /locus_tag="OQL90_002854"
     CDS             complement(11534..>12571)
                     /gene="fucP"
                     /locus_tag="OQL90_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1369565.1"
                     /translation="IITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETA
                     ANPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPE
                     QLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSR
                     LARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIG
                     RFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIF
                     SLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFI
                     FARFRSQTATN"
BASE COUNT         3012 a         3139 c         3329 g         3091 t
ORIGIN      
        1 cgtccgtgga tgcgcactat actccgctcg cgttttcatg caaccttttt tttaccccga
       61 tcacggattt agcatgattt tgaacaaaaa caaaccacta aagcctattt tttgtgcaaa
      121 atttgcgtca gggagaggct gctcatttca ttaaacgcgg cggtttgtta tcttcgttgc
      181 gccttatttt ttaacctgaa gaagagaaca atgaaaggac gttgggtaaa gtaccttctt
      241 atgggcacgg ttgtggcaat gcttgccgcc tgctcttcca aatcaaccga tcgcggacag
      301 caatataaag acgggaaatt tacccagcct ttctctctgg tgaaccagcc agatgccgtt
      361 ggcgcgccga ttaacgccgg tgattttgcc gagcaaatta accatatccg taattcgtca
      421 ccgcgtctgt atggcaacca gagtaatgtt tataacgcgg tgcaagagtg gctgcgcgca
      481 ggcggtgata cccgcaatat gcgccagttc ggcattgatg cctggcagat ggaaggtgcc
      541 gacaactatg gtaacgtgca gtttacaggc tattacacgc cggtaattca ggcgcgccat
      601 acccgccagg gcgagttcca gtatcctatt taccgtatgc cgccaaaacg tggtcgtctg
      661 ccgtctcgtg cggagatcta cgcgggggca ttgggcgata aatacatcct cgcttacagc
      721 aactctctga tggataactt cattatggat gtgcagggta gtgggtatat cgactttggt
      781 gatggcagtc cgcttaactt tttcagctat gcagggaaaa acggtcatgc ctatcgcagc
      841 attggtaagg tgctgatcga ccgtggcgaa gtgaaaaaag aagatatgtc gatgcaggcg
      901 attcgtcatt ggggcgaaac acacagtgaa gccgaggttc gcgagctgct ggaacagaac
      961 ccgtctttcg tcttctttaa accgcaatct tttgcaccgg tgaaaggggc aagtgcggtg
     1021 ccgctggttg gccgtgcttc tgttgcctcc gatcgttcaa ttattccgcc aggtactacc
     1081 ttgctggcag aagtgccgct gctggataat aacggcaaat ttaacggtca gtacgaattg
     1141 cgtctgatgg tggcgctgga tgtcggtggc gcaatcaaag gccaacactt cgatatctat
     1201 caagggatcg ggccggaagc cggacatcgc gcaggttggt acaaccacta tggacgtgtc
     1261 tgggtgctga aaaccgcccc gggcgcaggt aacgtcttta gcggctgatg tgttattctg
     1321 tataccgttt ttacggatac gacagggtga gggataacct cacccttttt aattctgagg
     1381 tgctatgtct gtggtaatta gtgatgcatg gcgtcagcgt tttggtggca cagcgcgtct
     1441 gtatggtgaa aaagcgttgc aactgtttgc tgacgcgcat atttgtgtgg ttggtatcgg
     1501 tggtgtcggt tcctgggcgg cggaagcgct ggcgcgcacg gggattggcg caatcacgct
     1561 tatcgatatg gatgatgtgt gcgtcaccaa taccaatcgg caaattcacg ctctgcgcga
     1621 taacgtcggg ctggcaaaag cggaagtcat ggcggagcgc attcgccaga ttaacccgga
     1681 gtgccgtgta acggtggtgg atgatttcgt gacgccggat aacgtagcgc aatatatgag
     1741 tgccggttat tcgtacgtga ttgatgccat tgatagtgta cgacccaaag cggcgctgat
     1801 tgcttattgt cggcgcaata aaatcccgct ggtcacgacc ggtggcgcgg gtgggcagat
     1861 tgacccgacg cagattcagg ttaccgatct ggcgaaaacg attcaggacc cgctggcggc
     1921 gaagttgcgc gagcgcctga aaagcgattt tggcgtagtg aaaaacagta taggtaagct
     1981 cggcgtggat tgcgtgtttt ctactgaagc gctggtgtac ccgcagtcag acggtacggt
     2041 gtgtgcgatg aaagccacgg cagaagggcc gaagcggatg gattgtgcat ctggatttgg
     2101 cgcggcaacg atggtgaccg ccacctttgg gtttgttgcg gtttctcatg cgctgaagaa
     2161 gatgatggcg aaagcagcgc gtcagggtta aacaatcagg tcggatgcga cgcgaacgtc
     2221 ttatccgacc tggttctgtg cttaaacctg cttcgccgca gcgataattg cctcgcttaa
     2281 cgcattcaac ccctggctgc gtgaggcgct aagctgccca cgcaatccca gctcatcaaa
     2341 caatgccagc ggcgactgtg cctgtaattc ggcggcggtt ttcccctcaa cggcagtcaa
     2401 caacaccgcc aacaggccga gcacaatgcg gccttcgctg tcgccaaaga agtgcatttt
     2461 accgttttca gccacggtat atcccagcca gacgcggttt tcgcatccgg caatctcttt
     2521 agcctgcgct tttaactcgt ctggcaaagc cggaagctgt ttccccagca tgatcaactg
     2581 gcgatattta tcttcccatt gcgtcaacgg tgcgaaggta ttgcgtaacg tttctgcggt
     2641 tacggttgtg ccgaacggat gtccggcgaa ttgcgggttt gtcattaatc caccaataat
     2701 tccagcgcgc ggtcaacggc attcaccagc gcatccacat cactctttgt attatatggc
     2761 gcaaaagagg cgcgcagtgt gccggttacg cctaattctg ccagtagcgg ctgagcacaa
     2821 tgttgcccgg cccgcaaggc aataccgtac tccgccagta gcgtcaccat atcgctgtga
     2881 tgaacgccag caaaatcaaa ggccagcagg ctggaatcct ggcagcggaa tgaacgaaag
     2941 cctggacgtt tcgccagcgc atcttccgcc agcgttgcta agctatggct ccagttttcg
     3001 gcctggttga tatcgtaatc tgccagccat tccagcgccg cgcttaatcc tatgacacca
     3061 gccacatttg gcgttcctgc ttccagtttc cacggcgcag attgagtcgt gaagccgtca
     3121 aaactcactt cgtgaatcat tttgccgcca cccagccagg gcgacatcgc ttccagcagt
     3181 tctgatttgc catacagcac gccgataccc gtcgggccat acagtttgtg accagagaaa
     3241 gcatagaaat cgatatccag ttgctgaaca tccgcgggga aatgcaccgc cccctgagca
     3301 ccatcaacca tcaccaccat ccctgctgaa tgagcaaagg taatcgctcg cgccagatcc
     3361 gggcaaccgc cagtaacgtt cgacatctga ctcaacgcca gaatccgact acggggagta
     3421 atcagttctg gcaacaaatc gacatccggc agtcgctgcg cattaagcgg caatttcacc
     3481 actttggctc cagtttgttg ggcgaccatc agccagggga cgaggttggc gtggtgttct
     3541 gccacgctga caataatctc atcgcccggt tgcagacgcg gacgcgcata gcattgtgcc
     3601 accatgttga tggattcagt ggtgccgcgc gtccagacga tagttttatc atccggtgca
     3661 ttcagtaatt gcgccacttt ctctcgtgca gcttcataac gcgcggtcag gcgttgggct
     3721 tcggcaaact ggctgcgatg gacgtttccg gcgctcaggc tgtaaaactg tcgagtggct
     3781 tcaaccacgg cttcaggttt aagcgcggtt gcggcgctgt cgagatagac gcccgcatcc
     3841 tgtagtgcgg gaaactgggc gcgaaactgc gcgggattaa aaacgttcat ggtactcctc
     3901 ggcttgatgc accgatcgtg acgcaaaatt cactttgata caaggagagt gctaaccatc
     3961 agaattatct ggatatcctg gcgaagccgc gtaagtaaca tatgctgaat agtgttaggc
     4021 gttaatttta gcctgttaat caatactatt aagatttatt aatcgcttta aggagaagat
     4081 gatgaaaaag actgccgcaa ttatttctgc ctgtatgctg acttttgccc tgagcgcctg
     4141 ttccggttcg aactatgtga tgcacaccaa tgacggacgt accatcgtct ctgacggcaa
     4201 accacagact gataacgata ccggtatgat ttcgtataaa gacgctaatg gcaacaaaca
     4261 gcagatcaac cgtactgacg tgaaagagat ggtcgaactg gatcagtaat tcgcgatcgc
     4321 aaggtaaaaa aaagcaccgc aattaggcgg tgctacatta atcactatgg acagacaggg
     4381 taaatgtaca ggaagtgaaa aaaggtagct ttgctaccat ggtctgaatc gcagaccaat
     4441 tgcaaacaca acaacacaac atcacaaccg taagccaaaa gttcaccaga acacgcattc
     4501 cgataaaact tttcgttccg gctcaggaag tgccgccact ataggtattt gctggtagaa
     4561 gctcaacgga caatttataa tggctcagat taaaaaaact aataggttac atagtgtgat
     4621 ctaattgtta aattcattta acatcaaagt ttaatagcca tgtctaaacg attaccaccg
     4681 ctaaatgcct tacgagtttt tgatgccgca gcacgccatt taagtttcac tcgcgcagca
     4741 gaagagcttt ttgtgaccca ggccgcagta agtcatcaaa tcaagtctct tgaggatttt
     4801 ttggggctaa aactgttccg ccgccgtaat cgttcactcc tgctgaccga ggaagggcaa
     4861 agctatttcc tcgatatcaa agagatattt tcgcaattaa ccgaagcgac gcgtaaactc
     4921 caggcccgta gcgccaaggg ggcgttgacg gtcagtttac tccccagttt cgccattcat
     4981 tggttggttc cgcgactttc cagctttaat tcagcttatc cgggaattga cgttcgaatc
     5041 caggcggttg atcgtcagga agataagctg gcggatgatg ttgatgtggc gatattttat
     5101 ggtcggggca actggccggg gctacgggtg gaaaaactgt acgccgaata tttattgccg
     5161 gtgtgttcgc cgctactgct gactggcgaa aaacccttga agacaccgga agatctggct
     5221 aaacatacgt tattacatga tgcttcgcgc cgtgactggc agacatatac ccgacagttg
     5281 gggttaaatc atatcaacgt tcagcaaggg ccaattttta gccatagcgc catggtgctg
     5341 caagcggcta tccacgggca gggagtggcg ctggcaaata acgtgatggc gcaatctgaa
     5401 atcgaggccg gacgtcttgt ttgcccgttt aatgatgttc tggtcagtaa aaacgctttt
     5461 tatctggttt gtcatgacag ccaggcagaa ctgggtaaaa tagccgcctt tcgccaatgg
     5521 atcctggcga aagccgctgc tgaacaagaa aaattccgct ttcgttatga acaataattt
     5581 acgtagggta cgaccatgac cagccgtttt atgctgattt tcgccgccat tagcggcttc
     5641 atttttgtgg ctctgggcgc ttttggcgcg catgtgttaa gtaaaaccat gggagccgtt
     5701 gagatgggct ggatccagac cggcctcgaa taccaggcgt ttcatacgct ggcgatctta
     5761 ggtctggcgg tggcaatgca gcgtcgcatc agtatctggt tttactggag tagcgttttc
     5821 ctcgcgttag gcacggtgtt gttcagcggc agcctttatt gcctggcgct gtcccatctg
     5881 cgtttgtggg cgtttgtcac tccggttggc ggcgtgagct tcctcgcggg ctgggcgtta
     5941 atgttagttg gtgctatccg tttaaagcgc aagggcgtaa gtcatgaata aggttgtatt
     6001 gctgtgccgt ccgggctttg aaaaagagtg cgccgcagaa attaccgata aagccggcca
     6061 gcgggaaatt ttcggttttg cccgcgtgaa agagaattcg ggttatgtca tttatgaatg
     6121 ttatcaacct gatgatggcg ataagttaat ccgtgagctg ccgttcagtt cattaatttt
     6181 tgcccgccag tggtttgtgg tgggggaact cctgcagcat ttgccgccag aagatcgtat
     6241 tacccccatt gtcggcatgt tacagggcgt agtagagaag ggcggtgaac tgcgtgttga
     6301 agttgccgat accaacgaaa gcaaagagtt gctgaaattc tgccgtaaat ttactgttcc
     6361 gctacgcgct gccttgcgcg atgccggggt gctggcgaac tatgaaacgc cgaagcgtcc
     6421 ggttgtgcat gtattcttta ttgcaccagg ctgctgctat accggttact catacagcaa
     6481 caataattcg ccgttctata tgggcattcc gcgcctgaaa tttccggcag aggcgccgag
     6541 tcgttccacg ctcaaactgg aagaggcatt tcatgtgttt attcctgcgg atgagtggga
     6601 tgaacgcctg gcgaacggga tgtgggcggt ggatttaggc gcttgccctg gcggctggac
     6661 ctaccaactg gtgaagcgca acatgtgggt ttattccgtc gacaacggcc cgatggcgca
     6721 aagtctgatg gataccgggc aggtgacgtg gctgcgggaa gacggtttca aattccgtcc
     6781 gacacgcagc aatatctcct ggatggtatg cgatatggtt gaaaaaccgg ctaaagttgc
     6841 ggcattgatg gcgcagtggc tggttaatgg ctggtgccgt gaaaccattt tcaatctcaa
     6901 actgccgatg aaaaaacgct acgaagaagt atcacacaat ctggcgtata ttcaggcaca
     6961 gcttgatgaa catggcataa atgctcagat tcaggcgcgg cagttgtatc acgatcgcga
     7021 agaagtgacg gtgcacgtcc gccgcatctg ggctgcggtg ggtggtcgtc gcgacgagcg
     7081 ataacaggca aggccggggc gtcgtcttcg gcctgctacc ctttccgtca ggctgttacc
     7141 aaagaagttg caacctggcg ctcatcagaa ataatgtgcg ttacctcatc cagatgcccg
     7201 atgcgggctt cccctgaacg attaaattta cttttatcaa tcaataacaa cgattgcgcg
     7261 gcacgtttta atagcatcga tttgtaatca gcgttgatcg cattggagtc ccacagtgcg
     7321 ccgctgctat cgatcccttc acaagaaaaa ataaacagat cgatttccag cgatttaagt
     7381 tgggaaatca gcgagggatt gacgtaacag ccatatttgc gctcaagtgt gccgccggaa
     7441 ctgatcagtt gaatgcgttc gcgtttaccg agttcatagc aaatcggatg gctattggtg
     7501 aagacctgaa tgttgatgtc aggcaactgg cgtgccagat accagcaagt tgaactggcg
     7561 tctaaggcta tcaccatccc ttcttcaatc cacgcgagcg cctcgcgcgc gatatctgct
     7621 ttatgcgcat aatggctttt cagcctgatg tgaaagggat cgccactgtc ttgattttga
     7681 cggtggatat atttagcgcg tccatgattg cgcagaattt taccctgcgt ctgtaattca
     7741 ttgagatcgc gacgaatggt ttctttactg acctttagct gttcagagag agcttccgtg
     7801 gtcaggctgg tatggttcag cagcaggtcg actatcgctt gctggcgtgc cgctttcata
     7861 tttttcaccc catcattcga taaccgcccg cgtcatgcgg gcgtaccggt atgagattac
     7921 ggtgttaccc ctttttttaa aagaatattc ccgtacttcg ctcgttcgcc tgtgataacg
     7981 atcgcaaagg ctttttgcgc ccgttcataa aacgcaaaac gattgatgcg gatgatgtca
     8041 ggacacgggg cttgtagtga aagcgcatta cggtaacgtc gttctacttc aggatcgaga
     8101 gtgtcacctt ctaccgccgc catcatcacc agcggcggtg cataactgtc cagttcaaat
     8161 aacgggataa tcgcctggag caagtcgctc accaacaggc catcagcgcg gatcacctgc
     8221 ggtcccatcg aatgggcggg aaagtgagca tcggaaaaaa taatttcatc tccatgtccc
     8281 atctctgcca gcactttcaa tagttcggga gaaattaacg gcgaaattgt tttcagcatt
     8341 tcacacttcc tctataaatt caggttcagt ttgcgggtag aaataacggt actgataatg
     8401 aatctgtgcg cgggcttctt cagggctgtt aaattcccct acgccatacc aaccgaacag
     8461 cgcagctcct gcgacggtcg tttcggcgtc gtcgagaact tttaccggaa tatcaagcat
     8521 attggcttta atctgattcc acaatgtgtt gcgacttcct ccaccgacta acaataattc
     8581 agaggcctta aagtgcccga ttttttccag catctgtaga ttgcgctgta attgcgcagt
     8641 taacccttcc agcgccgcgc gatagaaatg cccccgcgtg gtattaagcg tcactccttg
     8701 ccagccagcg ttctgacacg acaataaatc acactgcatt tttacgccat ccgcgctagg
     8761 cgcgataaga cgagcttctt caatcaacat ttgccagggt gtttcagccg tccagaacag
     8821 ttttctcacc cattccagca cgccggatgc cagccattgc atacctgggt tatacaaccc
     8881 tgcctggcta tccagttcgc aggtggaacc ggcgtactga cttaacagcg aagtatcaac
     8941 ctgggcgcta cggactatta aaatttccca tgtaccggaa gagagcacgg gttcattttg
     9001 gtcggcacca gcgccaaaaa gggcgaactg ggtatcgtga cctgcggaaa tcaccggtat
     9061 gccaacgggt aagccgagca ttgctgcggc gctgttctgt agcgtaccaa tctgttcacc
     9121 cgcttccacc agacgaggga agagtcggcg tggaatacca gtggcttgta aaatttgcgg
     9181 actgaaatcg cgttgctgga tatccagcat ctggctggtt ccggccatcg tgatatcagt
     9241 agtgaattcg ccggttaaac ggtggttaat cagcgacgaa ataaagaacc aggcgtgcgt
     9301 gcgttccagc agttgtggat gattttcttt caaccacacc aacttatata acgtattgaa
     9361 actaaaggct ccgacgccag aaatagcctg caaccgctgt gcggagatca actgttcaat
     9421 attgtccata accgctgctg ttcgcggaca tttccagcta atgatcggat agagcagatt
     9481 gccttgctta tctaccagag cgccatccac accaaaggtg gtgacggcga taccgcggat
     9541 gtggcattca gtcagttcac tattgatttg ccgacagcaa tcagcaaagc gttgcaaaat
     9601 ggcgtctaaa gaccactggt gccaggtgtt gttttccatc gcgatatcgc tggcattagg
     9661 cgttgaggcg cgggcaacaa tttttccctg ccgattaacc gcgatggccc tgacattggt
     9721 cgcgccacag tcgagtacca ggataacttc ttgtttcata atcgctcctg caatacttca
     9781 atatccatgc cgtgcgcagc ccaggcagaa ggacgataca ctttggtctc ttcaacgttg
     9841 tgcatacata ccgggatacg cagcatggag gcgagagtga taaagtcagc gccaacgtgg
     9901 ccgatggtca gaaccccatg gttagcgccc cagttcgcca ttaccgagta cacatccgta
     9961 aacggccctt taccggtgag gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg
    10021 cgtttgttga ggatgtcatg cacatccttc ggcaattcca cgctccagcc ttccgcgatt
    10081 tgcagtaccg gtcccaggcc tttgatgatg ttgacacgag tcatggtgaa cgggacgccg
    10141 ccttcggtaa ggaagcggga agagtaaccg ccgccacgga agtattcgtg gatcgccggg
    10201 caccattcgg tagcggcgag gcaagcgtca gcctcttgct gagagatttc ccagtgcggc
    10261 ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg aaccgtccag cgcagcagaa
    10321 cctgagttga tcaaatggat gatgccgtgt tctgccagtc catcgagttt atgccccgtt
    10381 acacgctcaa ttgcttctgg tgaccagtag gtacgcacat cggcaaatac ctgagcggtg
    10441 ccggtgagct ggtgacccat tagcattgcc acgccgttaa gactgtcgtt ttcggtcgcc
    10501 acgacaaagg gttcgcgcac gccattccag tcaaatgaac tgttgaggat cgcttcggcg
    10561 gtgtcaccat tgggatattg atcggtccag tgacgttgcc cctggaagcc cgcagcgatg
    10621 gcgttgtagc caagtgattc ttccacgcga ccaatatcgg ccagtttgct gttgccttgc
    10681 atcatgtcgc ggatacacat cgccatcagt aaactttcgc gcagaactgc gcggctttgc
    10741 tcggcattgc gttgatactg tttgttattt tcatcttcgc catagcggaa gtttttatca
    10801 gcccaggcca gtgccatttc cagttcggct tcgtcgtaaa tcttctgatc gatacggcga
    10861 cgcagttcgg tcatatccac cgcctggact ttcattccca gccaggattc aaagaagttg
    10921 tgatcaacga tggaaccggc gatacccatc gaaacgccgc ccagcgacag atagctttta
    10981 cctttcatgc tggcgacggc caaaccggcg cgggcaaagc gcagcagttt ttcttcaaca
    11041 tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc
    11101 gggatgcctt tctggctgtg agctgccaga gccgctgcca ggtaaacagc gccgggacgt
    11161 tcagtgccgt taaagcccca aatggctttc gggcgggttg gatccatgtc gatggtttca
    11221 ctgccatagc accagcaagg cgttaccgta atggtgaggc ctacattctg gctgctgaat
    11281 ttttcttcgc aagcagcggc ttcagccata cccgcgatac aggtatcgga aatgacacac
    11341 tcgacggcag ctccgcaggc atggcgcagt ttctcggtca gcagtgcggc cgtagctttc
    11401 gccatattca ttgtttgttc ttcaagcgac tcacgaacac ccatgcgacg accgtcaata
    11461 accgggcgga taccaatttt cggtaagctg atttttttca ttacagattc ctcactttat
    11521 tcggaaaata tgttcagtta gttgccgttt gagagcggaa acgggcaaag ataaagatga
    11581 ccgcgaagca gagtgcgggg atcagttcag cagtggggat gttgcccgcc gcgtcactga
    11641 caaaacccat gaccggagtg acaataccgc cgccaataat ggtcataacg atgaaggacg
    11701 aaccgtattt ggtgtcctgg ccgagattct taatgcccag cgagaagatt gttgggtact
    11761 gaatcgacat aaaggcgctg cataaagtca gggctattaa gcccacatga ccgccagcga
    11821 aggctgagat caggcacagt gccatagcga ttaatgcgta ggcggccagg actttgtgtg
    11881 gtgcgaagcg actgatgagc caggtaccgg tgaaacgacc aataaagaag cacaccatgg
    11941 ttccggttaa atagttagcg gcaaagcctg cagtcatacc tggaatttct tctacagcgt
    12001 agcgaatcaa atagctccag caggccgttt gtgcgccaac gtagcagaat tgcgctaata
    12061 ctgcccagcg ccagtggcga atacgcgcca ggcgagaaag cgatgcggag aacgatcctt
    12121 gtttggcgtc actgtgatta tcactctgca atgccgggaa tttcgtcagc atgatcagca
    12181 gggcgaccag taacacgata gccacgatga tcatataagg tgtctgtacc gataatacca
    12241 ggctgtgttt atacgcactc aattgctctg gagacatttt atcgagaacg tcttgcgatt
    12301 gatgtggcac gttagacaaa ataagacttt gcccaaagac aaccgcgata attgcgccaa
    12361 acgagttaaa tgtttgcgca agatttaagc ggaagtgacc actgctttcc ggccctaata
    12421 ccgtaacaaa agggtttgcg gcagtttcca gacaacctaa tccggctgca ataataaata
    12481 ggccaactaa aaataaggtg tagttcatta tttctgcagc gggccagaat aatgcagcgc
    12541 ccagggcata taaaaataac ccagtaataa t
//
LOCUS       DAJVOY010000117        12464 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVOY010000117 DAJVOY010000000
VERSION     DAJVOY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.117"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(94..1185)
                     /gene="ychF"
                     /locus_tag="OQL90_002855"
     CDS             complement(94..1185)
                     /gene="ychF"
                     /locus_tag="OQL90_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1369566.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1302..1886)
                     /gene="pth"
                     /locus_tag="OQL90_002856"
     CDS             complement(1302..1886)
                     /gene="pth"
                     /locus_tag="OQL90_002856"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1369567.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2164..2442
                     /gene="ychH"
                     /locus_tag="OQL90_002857"
     CDS             2164..2442
                     /gene="ychH"
                     /locus_tag="OQL90_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1369568.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2497..4176)
                     /gene="dauA"
                     /locus_tag="OQL90_002858"
     CDS             complement(2497..4176)
                     /gene="dauA"
                     /locus_tag="OQL90_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1369569.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(4301..5248)
                     /gene="prs"
                     /locus_tag="OQL90_002859"
     CDS             complement(4301..5248)
                     /gene="prs"
                     /locus_tag="OQL90_002859"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1369570.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5399..6250)
                     /gene="ispE"
                     /locus_tag="OQL90_002860"
     CDS             complement(5399..6250)
                     /gene="ispE"
                     /locus_tag="OQL90_002860"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1369571.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6250..6873)
                     /gene="lolB"
                     /locus_tag="OQL90_002861"
     CDS             complement(6250..6873)
                     /gene="lolB"
                     /locus_tag="OQL90_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1369572.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7087..8343
                     /gene="hemA"
                     /locus_tag="OQL90_002862"
     CDS             7087..8343
                     /gene="hemA"
                     /locus_tag="OQL90_002862"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1369573.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8385..9467
                     /gene="prfA"
                     /locus_tag="OQL90_002863"
     CDS             8385..9467
                     /gene="prfA"
                     /locus_tag="OQL90_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1369574.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9467..10300
                     /gene="prmC"
                     /locus_tag="OQL90_002864"
     CDS             9467..10300
                     /gene="prmC"
                     /locus_tag="OQL90_002864"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1369575.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10297..10689
                     /locus_tag="OQL90_002865"
     CDS             10297..10689
                     /locus_tag="OQL90_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1369576.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10693..11502
                     /locus_tag="OQL90_002866"
     CDS             10693..11502
                     /locus_tag="OQL90_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCS1369577.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11538..12392
                     /gene="kdsA"
                     /locus_tag="OQL90_002867"
     CDS             11538..12392
                     /gene="kdsA"
                     /locus_tag="OQL90_002867"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1369578.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3111 a         3157 c         3239 g         2957 t
ORIGIN      
        1 cggtgaaatg aaaaatccac gcaattgcgt ggattttata tacttttgcg ctcttcatga
       61 gatttagcga aacctcatga gacaataaat taattagacg ttgaaaagga agttcatcac
      121 atcgccatct ttcacgatgt aatctttacc ttctgcacgc attttgcctg cttctttcgc
      181 gccttgttca cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg cacggataaa
      241 gcctttttca aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg
      301 aatggtccat gcacgcactt ctttcacccc agcggtgaag taagtttgca ggttcagcag
      361 cttataaccg gcacggatca cacggttcag gcccggctct tccagcccaa gctcctgcat
      421 aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag cagcacaaac
      481 cggaaccaca acagaacctt ctttcgccgc gatttcacgc acctggtcaa gatatgggtt
      541 gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt gttggtttca gcgtcaggaa
      601 gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc
      661 tgcattttcc aactggggca ggcatttttc caggaccgcc agctcagctt tcgcgtcttt
      721 atcgccacct ttggctttct tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc
      781 tgccagcgcc agttcggtgt tgataacttc aatatcgtca gccgggttaa ctttgccgga
      841 aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc
      901 acggatgttg gtcaggaact ggttacccag accttcgcct ttcgatgcgc cttttaccag
      961 accggcgata tcgacaaatt ccatggtcgt gggaagcgta cgctgcggtt ttacgatttc
     1021 agccagttga tccaggcgag gatcaggcat tggtacgacg cctgtgttcg gctcaatggt
     1081 gcagaatgga aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga
     1141 tttcccgacg ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt
     1201 aatatcttaa taatcaacct gttattgcta acagattgca gaaatggaaa taactttgcc
     1261 tattatacac ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg
     1321 tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc
     1381 gcttcgtcaa tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca
     1441 aaaccgacaa ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag
     1501 ttagggttat tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca
     1561 ccgcccaatt taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga
     1621 atttcgtccg gattaatgcg gaaaaaactg gccatcgccg caacggcttt gccgctgaga
     1681 ttcataaatg tagtcgggac taacaggcgg acatcttcgc ctccaagagt gactcgcgaa
     1741 gtataaccaa agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag
     1801 tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc
     1861 aggccgacaa tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc
     1921 gtagtttact ggttgcgacc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta
     1981 ataattgcct gagtgggcta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt
     2041 tgttgaaata agggttgtaa ttgtgatcac gcccgcacat aacccacagg gtgttgtcta
     2101 tactttacac ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga
     2161 catatgaaac gcaaaaacgc ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg
     2221 gtaatggtgg tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc
     2281 cagtatttcg cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg
     2341 ggtgcccgtg ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgccactat
     2401 gacaaacgtt gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct
     2461 gaccagtcag tttgcgctga ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg
     2521 acgattcggg aagaacgcga ggcgtcccga gattggttga atgcctgcgc gagccatagt
     2581 gcgcagtggc tggaattcca cgttgcacac gcgcagttca catccttcgg gcagacgctt
     2641 cacaaaacgc tggaacgcat caagaccacc agcatcaagt accggaacgg catcccactt
     2701 cagaatcaca atccgtttgc cttcaagacg tgactccagg tccgtgaaca agccttcagc
     2761 agcagcaaaa aacagcgggc caataacgcg cagtaccaga acatcgtctg gaacatctac
     2821 gactaccggt gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag
     2881 cacgatcccc acgctgatgg caataaccat atcaaacagc acggtcagcg acatgcacag
     2941 cagcatgaca atgatgtcat ctttcggtgc atgacgcagc aagtcgacca ctttatgcgc
     3001 ttcactcatg ttccacgcca ccatcaacag cagggctgcc atagcggaaa gcggcagcca
     3061 ggagagcagc ggtgccagta ccagcagggc aagaataacc agaatagagt ggatcaccgc
     3121 tgagataggg gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagctgt
     3181 agcggtaata ccaccaaaga atggagcgat aatattcccc agtccctgtc caaccagttc
     3241 gctgttcgcc ttgtgtttcg tcccggtcat accatccagt accacggcgc agagcagaga
     3301 ttcgattgcg ccgagcattg ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc
     3361 ccaggttagc gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag
     3421 ttgcggaata ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat
     3481 ggtagcaaca tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa
     3541 ggccggaagg tgaccgggta aacgaatgcc cagacgcggc caaaaaacaa gaatacctag
     3601 cgtcacaatg ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc
     3661 gccgactttt tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt
     3721 aatctgcatg gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac
     3781 cggaatatac tcaatcaggc gaccaaagcg tgccagaccc atcagaatca aaaagatccc
     3841 cgacagcaag gccgcaacca gcagtcctgc cagtccaaac tgttgcgaca cgggatagat
     3901 aattaccaca aatgccgcag tcggaccgga aacgctaaag cgtgacccac ccgtcagagc
     3961 aatgacaatc cccgcaacag ctgcggtata taaaccgtac tggggtgcca caccactacc
     4021 aatagccaac gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag
     4081 gtcacgggta aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg
     4141 gaaaggcatc acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca
     4201 tcatctgtca actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga
     4261 caaaaaaacc cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag
     4321 agatcgattc ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca
     4381 gagtacgcac gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa
     4441 cgacttcatc aattacagag ttacgcaggt tgttcgccgc gttgccagag aagatcgggt
     4501 gagtcgcgta cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac
     4561 acagcgtacc gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt
     4621 caccgatgat atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg
     4681 ccatatcggt atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt
     4741 ccggagaaac cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg
     4801 ggctaccaaa tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca
     4861 gatccactgt cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg
     4921 cagtgattgg tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg
     4981 ggataacagc ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta
     5041 attccatcag gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac
     5101 cgcgtacatt ttcattaatt tgtacgctga cttcgccatc gctaaagcga cctacagcgg
     5161 cgtcgccgag tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt
     5221 taccagcaaa aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg
     5281 aaagaatctg ccgaaaactg tgcgggccag gcatgatcct ttccaggcag tgtattaaag
     5341 agcgcgatgc aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt
     5401 aaagcatggc tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc
     5461 attccggggc ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga
     5521 cacaggcccc tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg
     5581 catcaacctc gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt
     5641 ttagcaacgt ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa
     5701 tcaccggagt cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat
     5761 ccaccggcgt tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga
     5821 caggaacatc tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc
     5881 attgccagag atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga
     5941 gaccgccgcc catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa
     6001 gacgcccgct gtctgccgca gttttcatca acaatcgcgc tgcgcgaacg atcaggttat
     6061 cttcatgttc cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct
     6121 caatgctgat ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac
     6181 catccgcacg ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact
     6241 gtgtccgcat tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc
     6301 ggtgagttcc atattggctg gcatcgcagg atgcgttttg gtgtcataac caccataaac
     6361 aaccttccag tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc
     6421 cagtttgtag tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag
     6481 cggaattggc attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg
     6541 ctgaccttta ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt
     6601 gctgcccaat gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa
     6661 aaagcgggcg tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata
     6721 ctgattaaga ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt
     6781 gccaggacct ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag
     6841 caggcggata agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt
     6901 gcagttataa cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt
     6961 aattgccaga atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg
     7021 atcttacgca tcctgtatga tgcaagcaga ctaactctat caacgttggt attatttccc
     7081 gcagacatga cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga
     7141 gaacgtgtat cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag
     7201 ccgatggtgc agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc
     7261 gttgaagagc aggataacct gcaagaggcg ttaatccgct ggctttgcga ttatcacaat
     7321 cttaatgaag aagatctgcg taaaagcctc tactggcatc aggataacga cgcggttagc
     7381 catttaatgc gtgttgccag cggcctggat tcactggttc ttggggagcc gcagatcctc
     7441 ggtcaggtta aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg
     7501 gaacgcatgt tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc
     7561 ggtgccagtg ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa
     7621 tcgctctcta cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg
     7681 cgtcatctgc gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt
     7741 gcccaaattc tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa
     7801 cgtctgcgcg aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg
     7861 aaaggcatgg tggagcgcgc attaaaaagt cgtcgtaacc aaccaatgct gttggtggat
     7921 attgccgttc cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat
     7981 agtgtggacg atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg
     8041 gttgaggcgg aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca
     8101 caaagcgcca gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag
     8161 ttaaccgcca aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag
     8221 gatctggcat ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag
     8281 gccgcccgtg acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag
     8341 tagcagtaca tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg
     8401 ttgccaaact ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg
     8461 cgcaaactat cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa
     8521 gtgatgtttc gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac
     8581 agatgatgct cgatgatcct gaaatgcgtg agatggcgca ggatgaactg cgcaaagcta
     8641 aagaaaaaag cgagcaactg gaacagcaat tacaggttct gttactgcca aaagatcctg
     8701 atgacgaacg taacgccttc ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc
     8761 tgttcgcagg cgatctgttc cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg
     8821 tagaaatcat gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa
     8881 ttagcggtga tggtgtgtat ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc
     8941 gtgttcctgc tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa
     9001 tgccagaact gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata
     9061 ctttccgctc gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta
     9121 ttactcactt gccgaccggg attgttgttg aatgtcagga tgaacgttca caacataaaa
     9181 acaaagcgaa ggcgctttca gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac
     9241 gccaacaggc cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc
     9301 gtaaccgtac ttacaatttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc
     9361 tctaccgcct ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc
     9421 aggaacatca ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaaca
     9481 ctggttacgt gaagcaataa gccaacttca ggcgagcgaa agcccgcggc gtgatgctga
     9541 aatcctgctg gagcatgtta ccggcagagg gcgtactttt attctcgcct ttggtgaaac
     9601 gcagctgact gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg
     9661 tgaacccatt gctcatttaa ccggggtgcg agaattctgg tcgttgccgt tatttgtttc
     9721 gccagcgacc ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg
     9781 gttgcctgaa caaccttgcc gtattcttga tctcgggacg ggtaccgggg cgattgcgct
     9841 tgcgctggct agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc
     9901 tgtctccctg gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca
     9961 aagcgactgg tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc
    10021 gtatattgac gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac
    10081 tgcgctggtt gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg
    10141 taacgcgctg gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc
    10201 ggtgcgacaa gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg
    10261 tgataacgag cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt
    10321 agtgttcatc ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta
    10381 cgttatcaga agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc
    10441 gatacggtgt tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt
    10501 tccgggcagg cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg
    10561 ggttttattg cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc
    10621 ccgctggcgt tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta
    10681 ctggggtaag tcatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa
    10741 ggcatgatcc tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac
    10801 gaactggagc gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa
    10861 gagcagctgg aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc
    10921 ggcgtttatc gtctttccga tgcattatgg ctggaccagg tgttaaagaa tcgacagggc
    10981 agtgcggtat cattaggtgc ggttttatta tgggtcgcga atcgtctcga tttgccgctg
    11041 ctgccggtga ttttccctac gcagctgata ttgcgcattg aatgtccgga tggcgaaatt
    11101 tggctgatta atccttttaa cggtgaatcg ttaagcgaac atatgctgga cgtatggtta
    11161 aagggaaata tcagcccgtc ggcggaactg ttttatgaag accttgatga agctgataac
    11221 attgaggtaa tccgcaaatt gctggataca ctcaaagcct cgttgatgga agaaaatcag
    11281 atggagctgg cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat
    11341 gaaattcgcg atcgcgggtt gatttatgcg caactggatt gcgaacacgt tgcgttgaac
    11401 gatttaagtt atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg
    11461 caaataaata acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc
    11521 aagattaagg cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa
    11581 atgacctgcc gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga
    11641 tgcgcatttg cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca
    11701 aagcctcttt tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg
    11761 aagaagggat gaaaatcttc caagagttga agcagacttt tggcgtgaaa attatcaccg
    11821 acgttcacga accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc
    11881 cggcgtttct tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa
    11941 ttaacgtcaa gaaaccacag tttgtcagcc cgggacagat gggtaatatc gttgataaat
    12001 tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg
    12061 acaacctggt tgtcgatatg ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc
    12121 cggtgatttt cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg
    12181 gtggtcgtcg tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg
    12241 ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc
    12301 tgccgctggc taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga
    12361 aaggtttcga agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta
    12421 gcgttctgcg ttaagacttt tttcatgggt gccagataca aaaa
//
LOCUS       DAJVOY010000118        12154 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVOY010000118 DAJVOY010000000
VERSION     DAJVOY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.118"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(34..1743)
                     /locus_tag="OQL90_002868"
     CDS             complement(34..1743)
                     /locus_tag="OQL90_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1369579.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(1864..3255)
                     /gene="xanP"
                     /locus_tag="OQL90_002869"
     CDS             complement(1864..3255)
                     /gene="xanP"
                     /locus_tag="OQL90_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1369580.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            3535..4740
                     /gene="gltS"
                     /locus_tag="OQL90_002870"
     CDS             3535..4740
                     /gene="gltS"
                     /locus_tag="OQL90_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1369581.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(4774..6855)
                     /gene="recG"
                     /locus_tag="OQL90_002871"
     CDS             complement(4774..6855)
                     /gene="recG"
                     /locus_tag="OQL90_002871"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1369582.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(6861..7550)
                     /gene="trmH"
                     /locus_tag="OQL90_002872"
     CDS             complement(6861..7550)
                     /gene="trmH"
                     /locus_tag="OQL90_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1369583.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(7557..9665)
                     /gene="spoT"
                     /locus_tag="OQL90_002873"
     CDS             complement(7557..9665)
                     /gene="spoT"
                     /locus_tag="OQL90_002873"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1369584.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(9684..9959)
                     /gene="rpoZ"
                     /locus_tag="OQL90_002874"
     CDS             complement(9684..9959)
                     /gene="rpoZ"
                     /locus_tag="OQL90_002874"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1369585.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(10014..10637)
                     /gene="gmk"
                     /locus_tag="OQL90_002875"
     CDS             complement(10014..10637)
                     /gene="gmk"
                     /locus_tag="OQL90_002875"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1369586.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            10895..>12154
                     /gene="ligB"
                     /locus_tag="OQL90_002876"
     CDS             10895..>12154
                     /gene="ligB"
                     /locus_tag="OQL90_002876"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1369587.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVSL"
BASE COUNT         2709 a         3189 c         3328 g         2928 t
ORIGIN      
        1 gtacgtctga gtcgtacccg gcgagattga tgtttactgc accagctctg ccgacgtaac
       61 ttcaccgctg gaaacgatgc cagcattcat ggtttgagtc acttgctgct ttgcggcgtt
      121 cacggcatgc aattgcccgc taaccgtagg tttcagcggg gcattggcct gaatatctcc
      181 actggcggta agctgaatat tgccgtcgcc agtcaacggt aattcaggcc atccccattg
      241 ttgcaaaata ttcaccggca caccgcgtcc attcaggctg atatgggtct gacgttgtgg
      301 cgtttgtgaa acactggcag tggcttccag aatgcctttt tcagtaaatg cactcagttc
      361 gctgatattc accgtgctgc tgttggcggt cagcgccagc gacgggcgac gaacatcaac
      421 acgattgaat gtcgcggcgg cggcattcag attcgccgag ccactccaga cgccccattt
      481 atgatcggta accagcgtca ggttggcacc gtaaccatcg agcgtggtga gctgccacgg
      541 gaagtcaggg tcgatatcaa tgatcagatt gcggctggcg ctaaatctct tcagttgcag
      601 gctgtttaac caaccgggtg tcgtttccat ccacaactgt tgccagtttt tcggcaaggt
      661 atattccagc ccggcaattg ccgcatcatc aaggatcaac gttttcccgt cacgcagcca
      721 gttccctgac gttctgacca tacccccttc ccagcggctg gtgaactggc gcagcgctac
      781 gccctgcggg gaaaattccg cgtttataat cgggtcaaat aaatgcagcg aaccataaat
      841 gaactcgcta gcgttcatcg acagtttgcc ttcttgtgtc tgccagtcat ctttactgaa
      901 ggtcatgttg cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac cttgcaaatg
      961 agcatcgatc acttccaggc gaccaatctg caacgacggg acagagcgta atggcgcaaa
     1021 gaagtcggtt agcgattttt cgctttgtag acgtatatcc gccatgcgca ggttttccac
     1081 ttgccagctg ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc gggcgatgtc
     1141 ggcacccagg ttagtcagcg taacgcgatc gttatcaata ctgccttcaa tcagtacatt
     1201 ggtggcagga acatcattga gcgaaagcga tccggcacta aactgaatct gcgccttcgt
     1261 acccagcact ttaccggctt ttggtgacca cggaactacg ccgccattta cccgctgcgc
     1321 gctcagtttc cattcgctat tcgggctatt aaacgccata tcacgcagtt gcagacgatc
     1381 ggctttgaac ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt tttccagcag
     1441 gatggtatcg acatggcgtg gttcggtcag ttgccgactg cttagcgcaa tgtcgacact
     1501 tttggccacc agggtcgcgg gctgaccatc acgaccaaac gtgacattct ccagcacgat
     1561 atgagatggc gcggaaaaac ggtgatccat cgccccgaag gccagatgat agtcgctatt
     1621 ctcggaaacc catgcgctga tatgttctgc tccccagcgg gtttgcagaa gaaaatagag
     1681 gccagcgatc accactaaca gagcgatgag aatgtagaga agcagcttcc caataaattt
     1741 catggtcttc cattccgtga aatgcacata agagagttat gcacgattta cgcgcaatcc
     1801 tcaaggcggg aatggtgaaa gaggtgagac tgcggcggta attatcttac cgccgccagt
     1861 gaattactgt ttttctggtg ggaaaatcag attcagcacg atggcagtaa taccgcccgc
     1921 ggcgatcccg gaggagagca ggtttttcag ccattcaggg gcaaactgca aaatcagcgg
     1981 ctgctgagac acgcccagac caaccgccag cgacagcgcg ataatcagaa tcgcccgacg
     2041 gttcagcggc tcacgagaaa cgatacgcac accggaggcg gcgatggtgc caaacattac
     2101 aagcgttgcg ccgcccagaa ccggttctgg aatgtgttgt acaaaaccgc tcactgccgg
     2161 gaacagaccc agcacgatca acatcagcgc gacgacaaaa ccgacatagc ggctggcaac
     2221 accagtcaac tggatcactc cgttgttttg cccgaagcag gagttcggga aggtgttaaa
     2281 caccgccgaa acaaacgagt tcaggccgtt tgccagcacg ccgcctttca ggcgtttcat
     2341 gtacagcgga ccggacactg gctgttcgga aacgtcagag gtcgacgtga tatcgccaat
     2401 ggtttccagc gaagtgatca taaagaccag catcagcggc agcagcagac tccattcaat
     2461 gccaagaccg taatagagcg gcgttggcac cataatcagt tcttgcgtca tcggttcgtt
     2521 gctttctggc aacatgccca taaaccacgc cagcgcatat ccggccgcca tcgcaatcac
     2581 cagtgaggcc acgcgtaagt aagggttacg ttgacggtta agcaggataa ttaaggctaa
     2641 gaccacgcct gccagcagca gatttttcgg tgcgccgaag gtgttatcgc tcatggctgc
     2701 gtaaccgccg ccaatggacg ttaacccaac ctgaattagc gacaggccga taatcatcac
     2761 cacaacgcca gaaaccagcg gcgtaataat gcggcgcgcc agatgcagaa cgcgggagat
     2821 caccatctcg gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca tcatggtagg
     2881 aacatcagca ccaccggttt tcagcgcggt accgcccata atcagcgggg caacaaagtt
     2941 gaagctggtg ccctgaatag acaacagccc ggagccaacc ggaccccagg tcttaatttg
     3001 aataatcgat gccacaccgg aggcaaacag cgacatacta ataatgtgtt gcgtgtcttg
     3061 tgccggtaaa cccagcgcct ggcagattaa tagcgctggc gtgatcaccg caacgaacat
     3121 cgccagcaga tgctgacagg cggcaaacag ggtttgagga agcggcggac gatcttcaag
     3181 acggtaaatc agttcgctgt tttgagtctg cgcaaccggt tgcgcatttt ctgactcgag
     3241 ggtggaaaca gacatcgaaa acaatcccgt ggtagcaaag cgggcatttt agctgaccga
     3301 aagagaaaag caaacgtttg ccacatcctc atcaaaatat ttaaatgaga taaatgcact
     3361 tttttatata acttttggta tttttctgct taaaatccat gccatttgtt cattaacagc
     3421 tgagaatttg acccaggaaa gcataactcg atacccgtca tagttcatga agttgccact
     3481 gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat acaaaggagt aactatgttt
     3541 catctcgata ctttagcaac gcttgttgcc gcaacgctga cgttgctgct cgggcgtaag
     3601 ttggtccatt ccgtctcctt tttgaagaaa tacaccatac cggaacctgt tgcgggtggt
     3661 ttgttggtgg cgctggcgct actagtactg aaaaaaagca tgggctggga agtcaacttt
     3721 gatatgtccc tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg cctgaacgcc
     3781 aacattgcca gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat tgtggttgtt
     3841 ggtctgttgg tgatgcaaaa tgccattggc attggtatgg ctagcctgtt agggcttgat
     3901 ccgctgatgg ggctgttggc cggttctatt acgctttccg gcggtcacgg tacgggcgct
     3961 gcgtggagta aattgttcat tgaacgttat ggcttcacca atgcgacgga agtggcgatg
     4021 gcctgtgcaa cgttcggtct ggtgctgggc ggcttgattg gcggtccggt ggcgcgctat
     4081 ctggtgaaac actccaccac gccgaacggt attccggatg accaggaagt cccgacggcg
     4141 tttgaaaagc cggatgtggg acgcatgatc acctcgttgg tgctgattga aactatcgcg
     4201 ctgattgcta tctgcctgac ggtggggaaa attgttgcgc aacttttggc tggcactgct
     4261 tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg tgattctgag caacggtctg
     4321 tcaataatgg gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg taacgtaagc
     4381 ttgtcgttgt tcctggcgat ggcgttgatg gggctgaaac tgtgggagct ggcttcgctg
     4441 gcgctgccga tgctggcgat tctggtggta cagaccatct tcatggcgtt gtatgccatc
     4501 ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc tgcgggtcac
     4561 tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc gatcactgaa
     4621 cgctttggcc cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc gttctttatc
     4681 gatatcgtca atgcgctggt aattaagttg tatttgatgt tgccgatttt tgccggttaa
     4741 ccgatgaagc ggcggtcttc tcaccgccgc cttttacgca ttcgagtaac gttctgtctc
     4801 cggcatccag cgttctatca aggcttttgc ctgttgtggg taacgttcgt gaatatgtcg
     4861 cgccaggcgc tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa
     4921 ttcagcatta cccgtctgac gcgtgcctaa caattcgcca gggccgcgaa tctccagatc
     4981 tttttgcgca atcacaaaac cgtcgttact gtcgcgcagc acttgcaggc gaatttgcgc
     5041 cgttttagaa agcggcgttt tgtagagcag cacgcagtga gaagccaccg cgccacgacc
     5101 tacgcgcccg cgcagctggt gtaactgcgc cagacccaga cgctcagggt tttcgataat
     5161 catcaggctg gcgttaggca catcaacacc gacttcaata acggtagtgg caaccagcag
     5221 atgtagctca ccttgtttaa acgacgccat caccgcctgt ttctcggctg gtttcatccg
     5281 cccgtgtacc aggccaacat tcaattctgg tagcgccagt ttcaactctt cccaggtagc
     5341 ttccgccgcc tgcgcttcca gtaactctga ctcttcaatc aacgtacaaa cccagtacgc
     5401 ctgacgacct tcagttatgc aggcatggcg cacgcggtca atgatgtcgg tacggcgggt
     5461 atcaggaata gcgaccgtag tcactggcgt gcggcctggc ggcagctcat ctatcaccga
     5521 ggtatcgaga tcggcatacg ccgtcattgc cagcgtgcgg gggatcggcg tggcggtcat
     5581 gatcaactga tgcggatgga agccttgctg ctggcctttc tcccataacg ccagacgctg
     5641 atgcacgcca aagcgatgtt gttcgtcgat aatcaccagc gccagaccgt taaactgcac
     5701 ctgttcctgg aagatggcgt gcgtaccaac aatcatctgc acctgaccgc tggcgatggc
     5761 ttcctgctga gacagtcgcg ctttgccttt ctgcttaccg gcgagccagc ccacttcgat
     5821 accgagcggt tcaaaccagt tgcggaagtt attggcgtgc tgttcggcga gtaattcggt
     5881 tggtgccatc aatgccacct gtttgccgtg ggcaatcgca cgcagcgcgg cgagggcggc
     5941 gaccagcgtt ttaccggaac ctacatcgcc ctgtaccagg cgcatcatcg gcacatccag
     6001 cgccatatcg cgctcgatct ccgccactac gcgtgcctgt gcgcccgttg gcttgaacgg
     6061 taaggctgcg aggagtttat ttttcagcgc atcattggcg ctcagcggct gggcatgaaa
     6121 acgctgtgct ccggcacgta aggctaacat gctgaggttg tgcgccagca gttcttccag
     6181 aatcagacga cgttgcgccg gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg
     6241 tggcgggcgg tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt gtgacagttc
     6301 cggcggcagg agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa
     6361 tttacgcagc gtggcctgct ttacgccttc cgttgttgga taaaccggcg tgagcgtttc
     6421 ctgtaattct ggcgtgctga gatcgccctg cacgcggtat tccgggtgga tcatctccgc
     6481 accatattta ccgcgctttg cttcgccata agccagtaca cggcggcccg tcgccaggct
     6541 atttttcatt gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct
     6601 gatctggcag gtcatcatcc gccgaccgcc gaaggagata ttgcagttca gcacttcgcc
     6661 ttccaccgtg gcataaacgc ccggcagtag ttctccgatg gggtagagat gggtgcgatc
     6721 ttcgtagcgc aggggaaggt gtaagagtag atcctgcacg gtatgcaggt tgattttcgc
     6781 cagtttgtta ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga cagcatctaa
     6841 caggcgacct gtcatggcac ttaccctgca gcctgcatag tagcccacca gtcggcatca
     6901 gcttcgatct cgccttgctg attgacgtgg ggataaggca ggcctttgcg ttttgcgact
     6961 ttcgccagca ccggatagcc gccttcaaac aacaggcgtt gttgctctgc ttccggcaac
     7021 atgctgtttt cacgcaggta catgcctgca ttttgccgct gacgctgggc ttcgtaaaga
     7081 atgagggctg aggcaacgga aacattcagc gactgcacca tgccgatcat cggaatgatg
     7141 atgtcctgat ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc
     7201 aaaatgcagg tcgggcgagt gtaatcaatt tcgcggaaat cgacagcgtt atcagaaaga
     7261 tgggttgcca gaatctgcat gccctggcct ttgagatgag cgacggcatc gccaatggtg
     7321 cggtgtgttt tcacctgtac ccagctgtta ctacccgccg ctgccgaagc catggtgcgc
     7381 atgcggctac caggccagac ggcgtgaact tcatgtacgc caacggcatc tgcggtacga
     7441 ataatcgcag aaacgttatg aggtttgtgg acctgctcca tgcagacggt cagatcaggc
     7501 tgccgcctgg cgagcatttc gcagatgcgt gcataacgtg ttgggttcat aaaacattaa
     7561 tttcggtttc gggtgacttt aatcacgtct ggcatcacgc ggattttgcg catgatattc
     7621 gccagatgca cacggtcacg agcggtcaga cgaataaagg cgctgtagac gcgaccatct
     7681 ttctcttccg tattcaaact ttgaatattc gaagtcgtgg tgttaattgc cgccgtcagg
     7741 tttgccagcg caccctgatg attgaacatc tccaccttga tttcggtgat gaactcctgc
     7801 gccgtctctt tatcccattc cacagccata aacttctctg gctctttctg gtagccacgg
     7861 atgttacggc aggattcatg gtggatcacc agacctttac cggggctgac gtgggcgata
     7921 atcgggtcgc caggaatagg gcggcagcat ttcgcaaagg tgatcagcac gccatccgcg
     7981 cctttgatcg gcagatgccc gtggctttga gtcgccggtg gaatggaggc gtccccatgt
     8041 tgtagatttt tcgcgaccac cacgctcatt gcgttaccaa ggccgatttc tgccagcaga
     8101 tcgtcaagcg ttgccagctt catgcgatcc agctcgcgct gaatattttc ctgcgggatt
     8161 tcattgagct tacggctacc acccaaagca tggttgagca gacgacggcc caggcttaca
     8221 gaatcatcac gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc tttcgagcta
     8281 acgacaaagt tcagccaagc ggcattcggg cgagcgcccg gagcggtaat gatttcaacg
     8341 gtctgaccgc tagtaagcgg ctgcgacagc gggtaaggct ggcggtcgac gcgtgcgccc
     8401 acgcaggcat ggccgatatc ggtatgcact gcataagcga agtcgacggg cgttgcaccg
     8461 gcaggcagct cgacaatgcg cccttccggt gtgaaaacgt aaatctcatc cgggaagaga
     8521 tcggatttaa cgctctcgat aaattcaaac gaactaccgg cgctctgttg cagctccagc
     8581 aggctttgca tccagcgctg ggcgcgtatt tgtgcggtag tactggtttc gccgtgctct
     8641 ttataagccc agtgcgcggc aacacccatc tccgccatct gatccatatc ttcggtacgg
     8701 atctggacct caaccggcac gccgtgcggg ccaatcatcg aggtgtgcaa cgactgatag
     8761 ccgttcgctt ttggaatggc gatatagtct ttcacgcggc ccggacgcgg cttgtacagg
     8821 ctgtgcatct ggcccagcac gcgataacag gtgtcagaat cattgacgat cacgcggaaa
     8881 gcgtagatgt ccatgattga gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc
     8941 gaataaagat gcttttcgcg accactgacg cggcacggta ttcccgcttc ctgcaaacgc
     9001 ccttcgattt cagaaagaat cttctggatc atctctttac ggttgccgcg cgcggctttc
     9061 accacttctt tgattacgcg ataacggttg ggatacagcg cctcaaaacc cagctcttcg
     9121 agttcggttt taatgtggtg gatacctaaa cggtgcgcca gcgggctgta aatttcgaga
     9181 gtttcacggg cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg
     9241 tgggtacggt cggcaagttt gatgaggatg acgcggatat cctgcaccat cgccataatc
     9301 atcttgcgaa agttttcggc ctgcgcctct ttcttatcgc ggaacttgag tttatcaagt
     9361 ttcgacaccc cctctaccag ctcggcgacg cttttaccaa aaagctgttc catatcctgg
     9421 taggtggcgg gagtatcttc aatcacgtca tgcagcagcg ccgccatcag cgtttcatag
     9481 tcgagtttca tctcggccag aatgcaggca accgctaccg ggtgcgtgat atagggttca
     9541 ccgcttgaac gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga
     9601 cgcttgattt ggtcttccgg caggtaggtt tgaatcagtt gattcaggct ttcaaacaga
     9661 tacaagggcg acccgctttg tgattaacga cgaccttcag caatagcggt aacggcttgt
     9721 aattcagcgg cttcctgctc ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg
     9781 ttgatcagac cttcttcgat ttcgcgcagc gcgattacag tggttttatc gttttcttcc
     9841 ggtaccagcg gatcctttcc gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt
     9901 accaggtcaa aacggttacc aattttctct acagcgtcct gaacagttac gcgtgccata
     9961 cttaaaaagc tccacaggtg aagaaatgac tgggcatgat actgaaatca ggttcagtct
    10021 gccaacaatt tgctgattaa agcgtcatga cgctgctttt ggcggctcat gcgcagacgt
    10081 tcggcgcgaa taatggtctt caaatcggtc aacgcggtat cgaagtcatc attcacaatc
    10141 aggtaatcat attcgtcgta atggctcatt tctgcaacag cttgcgccat acgctttgca
    10201 atgacctctt cgctgtcctg accgcgaccg cgtagacggc ggtccagttc aattttagac
    10261 ggcggtaaaa taaagatact ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcgccc
    10321 tgccagtcga tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc aatggcctca
    10381 cgcgaagtgc catagtaatt accaaaaact tctgcgtgtt cgaggaacgc atctctgcta
    10441 atcatttctt taaattcatc atgattaaca aagaaataat gttcaccgtg gacttcacca
    10501 ggacgcggtt ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt
    10561 tttaataaag cctgaatcag gctggattta cccgcgccac tgggggcaga aacaatataa
    10621 agcgtgcctt gagccatgag tatctttcgt atgtgattag cgaaataaag cctacatacg
    10681 agcttattat acacggcgcg gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag
    10741 ttgttgcgtt ttgcgtagct gcttccgcat ttgcctgtga tttactgcaa taaagacgat
    10801 tctcaggaag cgtcctcgca gactccgttt ttgccactcg gctttgcctt caccacgttg
    10861 tatatctgcc agcctgacta cagcggaggt gaagatgaaa gtatggatgg cgatattaat
    10921 aagtatcttg tgctggcaat catctgtgtg ggcggtctgt ccggcctggt cgccagccag
    10981 agcacaggaa gaaattttcc gccagcaaca gcaaataaaa cagtgggacg atgactactg
    11041 gaaggaagga aaaagtgagg tggaagacgg tgtttacgat cagttaagcg cccgtcttac
    11101 gcagtggcaa cgctgctttg tgagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg
    11161 tgcggttatg catccggttg cccatacggg tgtacgcaaa atggcggata aaaacgcatt
    11221 aagtctgtgg atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc
    11281 ggtaaccctg gtttatcggg acgggaaact gaacaaagca atcagtcgcg gtaatggcct
    11341 gaaaggcgag gactggacgc agaaagttag cttaatttcc gctgtgccgc aaaccgttag
    11401 cgggccttta gccaacagta cgcttcaggg ggaaatattt ctccagcgcg aggggcatat
    11461 ccaacaacaa atggggggaa taaatgcccg cgcaaaagtt gctggcttga tgatgcgcca
    11521 ggacgatagc gacacgctga attctctggg cgtttttgtc tgggcatggc cggatggacc
    11581 gcagttaatg tctgatcgtt taaaagagct ggctaccgca ggttttactc taacgcagac
    11641 gtatacccgt gcggtgaaaa atgctgatga agttgcgcgc gtacgcaatg agtggtggaa
    11701 agcggaatta cccttcgtca ccgatggcgt agttgtacga gcggcgaaag agccagaatc
    11761 ccgccattgg ctaccgggcc aggcagagtg gctggtggcc tggaaatatc aacctgtagc
    11821 tcaggttgcc gaagtgaagg cgattcagtt tgcggtgggt aagagcggta aaatatcggt
    11881 ggttgcgtca ctcgcatctg tcatgctgga tgataaaaaa gtccagcggg tgaatattgg
    11941 ttccgtcagg cgctggcagg agtgggatat tgcgcctggt gatcagattc tcgtcagcct
    12001 tgccggtcag ggtattcctc gcattgatga tgtggtgtgg cgcggtgcag aacgtacaaa
    12061 accgacaccg ccagaaaacc gctttaactc gttgacctgc tactttgctt ctgatgtttg
    12121 tcaggaacag ttcatttcac gcttagtcag tctg
//
LOCUS       DAJVOY010000119        12047 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVOY010000119 DAJVOY010000000
VERSION     DAJVOY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.119"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..182
                     /locus_tag="OQL90_002877"
     CDS             <1..182
                     /locus_tag="OQL90_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1369588.1"
                     /translation="FISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGTNTLL
                     ACCCLLRPEEVNSEY"
     gene            172..345
                     /locus_tag="OQL90_002878"
     CDS             172..345
                     /locus_tag="OQL90_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCS1369589.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(394..1466)
                     /locus_tag="OQL90_002879"
                     /pseudo
     CDS             complement(394..1466)
                     /locus_tag="OQL90_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1538..4282)
                     /gene="torS"
                     /locus_tag="OQL90_002880"
     CDS             complement(1538..4282)
                     /gene="torS"
                     /locus_tag="OQL90_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCS1369590.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4365..5354
                     /gene="torT"
                     /locus_tag="OQL90_002881"
     CDS             4365..5354
                     /gene="torT"
                     /locus_tag="OQL90_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1369591.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5327..6019)
                     /gene="torR"
                     /locus_tag="OQL90_002882"
     CDS             complement(5327..6019)
                     /gene="torR"
                     /locus_tag="OQL90_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1369592.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQT
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6139..7311
                     /gene="torC"
                     /locus_tag="OQL90_002883"
     CDS             6139..7311
                     /gene="torC"
                     /locus_tag="OQL90_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1369593.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7311..9899
                     /gene="torA"
                     /locus_tag="OQL90_002884"
                     /pseudo
     CDS             7311..9899
                     /gene="torA"
                     /locus_tag="OQL90_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9896..10495
                     /gene="torD"
                     /locus_tag="OQL90_002885"
     CDS             9896..10495
                     /gene="torD"
                     /locus_tag="OQL90_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCS1369594.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(10588..10893)
                     /gene="cbpM"
                     /locus_tag="OQL90_002886"
     CDS             complement(10588..10893)
                     /gene="cbpM"
                     /locus_tag="OQL90_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1369595.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10893..11813)
                     /gene="cbpA"
                     /locus_tag="OQL90_002887"
     CDS             complement(10893..11813)
                     /gene="cbpA"
                     /locus_tag="OQL90_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1369596.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2891 a         3095 c         3193 g         2868 t
ORIGIN      
        1 tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat aacctgttga
       61 ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg gtaccgggaa
      121 ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac agtgaatatt
      181 gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca aaaaatcgcc
      241 gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc acgagaaaaa
      301 atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac
      361 cccgtgcgag gtgatgttcc gcttgttgct aatttagtga tcaatcattg gcgcttgtgg
      421 aattaagcgt cggtacaatt cctccggcac cgggctttgc catactcccg catacattgc
      481 gtaaccaatc accgcgaaca taatccccag aaccagtagc gtcattaacc agccagacaa
      541 cgcaaaagct tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg ttgaggctac
      601 cggacatgac tctacgcaag tcatacagcc ggtacattcc actgttcgta cctgaattaa
      661 tttatctacc gggatccgtg atgggcaatt ttttgcgcat ttgccacagc cgatacaact
      721 ttcggcattg cgacgaatct taaacggcga taatagcgaa accacgccca tcagcgcgcc
      781 atatgggcaa agataacgac accaggcatg gcgaataaac aggctggcaa tcagcaaaac
      841 ggtcacgctg attaatgtcg cggtccccat atgacgaaag aaatcgagca tttttacgtc
      901 catcaccacg ctgtagggcg acaacataaa atagtgaatc gcctgagcgg gcatcaataa
      961 cgcgatatag agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag gaatatccag
     1021 ccagcgggga aggacacatt gccgaccaaa cagtttgtta ccgagatcgc cgattaattc
     1081 agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc tttttgagta atagactgat
     1141 gacgataaaa gcgaccaata acagcatcgc ggagcgtgga cggacggtaa ctgacctgtt
     1201 gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc gatacctccc
     1261 ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca ataccagaac
     1321 gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt acgccaggtc
     1381 gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc
     1441 cagcgggttc tttttttctc tgccatgatt ttgccagtcc gttaagttgc ataccaaatg
     1501 ccactatttt agttgttctt aactggctga tattgattca aatcgcgttc aggtctttct
     1561 tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt ggaagggggg
     1621 cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc attcccaaac
     1681 tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta tcctgactgg
     1741 cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat aataccagcc
     1801 attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc gatacatcca
     1861 gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga ccgagcactt
     1921 cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc tggcgcagcg
     1981 tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat tgctgtgcca
     2041 gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc agtgcggcag
     2101 caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca acagcaacaa
     2161 cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc gggttatctt
     2221 caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt tttggcacgg
     2281 gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc tccggcgtgc
     2341 tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata gtcagtccca
     2401 gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt tctgccagtt
     2461 tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc tcgccatcag
     2521 tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg ctcaacaggt
     2581 tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcggtcggt acatcgtcgg
     2641 caattgctgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt aattgcaggg
     2701 tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca ttcttgccac
     2761 ctgcttcgat agccgaataa tcgagaatat cgttgaggat ggtcagcaac gattcgccag
     2821 agtcggtaat cgcccgcaaa tcatcacgct gggcgttaag tgcggggtta tctgccagca
     2881 gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc atcgccgcca
     2941 gaaaggccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt cggtgttcta
     3001 tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc tgttcacggt
     3061 ggcgattcag tgcatgaaca gtgctgcgaa acgcatccat cagccgcccg atggtatcca
     3121 gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc agcagccgtt
     3181 gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc acgcgccaaa
     3241 ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg tattgcccgc
     3301 gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca atggtgtcga
     3361 ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt tgtgcgagag
     3421 tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa tcgctatatt
     3481 ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct tcaatgcgta
     3541 tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc attggtgcat
     3601 ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc agagcgctaa
     3661 ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc cgatcgagtg
     3721 cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg gcctgggtcg
     3781 ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg atctcatcgg
     3841 cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgtagttgc agacgccgcc
     3901 ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt tgttcaatag
     3961 cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc ttcaggcttt
     4021 gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca ctggtcaggt
     4081 tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc atattcatgg
     4141 taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc aggttgtacc
     4201 atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc atccagagtc
     4261 ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt gagtcgcaaa
     4321 gcagaatgca tctagcataa agccttatta ttgattaggc tatcatgcgc gtactgctat
     4381 ttttacttct ttcccttttc atgttatcgg cattttcggc tgataacctg ttgcgctggc
     4441 atgatgcgca gcattacacg gtgcaagcct ctacgccgct taaagccaaa cgcgcatgga
     4501 aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg aactatggta
     4561 tgcaggaggc aggcggctac agccagttgg ctacccagca agcacaaatc gaccagtgta
     4621 aacagtgggg cgcagaggcc attttgctcg gtagtagcac tacgtcattt cccgacctgc
     4681 aaaagcaggt agcaaatctg ccggtgatcg aactggtaaa tgctcttgat gatcccctgg
     4741 tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg cgatatttgg
     4801 tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc gataatgccg
     4861 ggggcagtaa agagatggtc gagggttttc gcgcagccat tgccggaagc ccggtgcgta
     4921 ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg ttgcaggaga
     4981 tgctggaacg ccacccagaa atcgacgtcg ttgccggaac agccattgcg gcagaggcgg
     5041 caatgggtga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt tatctttcac
     5101 atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc gatcaaatga
     5161 tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg caatcggtgt
     5221 ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac cgtgaacata
     5281 ttcgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag cacacatcag
     5341 cggctaagaa ataacattca ccatgttgcg tcaccagtaa atccgcgctg agtttatgac
     5401 gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc cgacgcgcag
     5461 aaagcatacg tagcagacgt tcacggctga gaatttcgcc cggattcgtc acaaatgcca
     5521 ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc tccagcgtat
     5581 ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga gtgtgcggtt
     5641 gagcttgtcg cgcgaggtcg attcgccaga gcagattttt cacccgtact accagttcgc
     5701 gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca acaatacggt
     5761 caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt tctcgcaggg
     5821 cgcgggttaa catcaggcca ttttcatcgg gtaagttgat atccagcaga attaaatcta
     5881 ccggctgagt ctgcataatt tcccgcagcc cggcaccgct cgctgtaacg gaaacggtat
     5941 acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca tcttcaacaa
     6001 taacaatgtg atgtggcatc agagggtttt actcattctg ttcatattct gccgtaagcc
     6061 gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc actaaaccat
     6121 aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc cagtgctcgt
     6181 tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct gattgtattg
     6241 ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg ccacagtatg
     6301 caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc cggcgtgcga
     6361 gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa gcgcaaactg
     6421 gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac acctgaaaaa
     6481 ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa agaaaacaac
     6541 tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa gcagcatcct
     6601 gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat cgactgtcat
     6661 aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca gttcgatgag
     6721 ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat taagccaatt
     6781 tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc ggaagtgaaa
     6841 gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac ggaaagcgcc
     6901 ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc ctcgattcgt
     6961 ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga caccaatacc
     7021 gagtggagca agttgcaggc cactgcgtgg atgaagaaag gtgatatggt gaacgatatc
     7081 aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca gtgccacggc
     7141 gcaccggaaa tctctcactt tgatgctaac ggctggattg gcacgctcaa cggcatgatt
     7201 ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct gcaaatgaat
     7261 gcgtctgaca tcgcaggtaa ggctcacggc gataagaagg aagaaaaata atgaacaata
     7321 acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc ttaacagtcg
     7381 ccgggatgct ggggccgtca ttgttaacgc cgcgcagtgc gactgcggcg caagcggcgg
     7441 atgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc cgcgcgacgg
     7501 tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat ccgtcgaaaa
     7561 tgattgccgg attgccggat tgccggattg ccggattgcc ggattgccgg attgccggat
     7621 tgccggatca cgtacacaac gcggcgcgta ttcgttatcc gatggtacgc gtggactggc
     7681 tgcgtaagcg ccatctcagc gatacctccc agcgcggtga taaccgtttt gtgcgcgtga
     7741 gctgggatga agccctcgac atgttctatg aagaactgga acgcgtgcag aaaactcacg
     7801 ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc cataacgctt
     7861 cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg ggcggagatt
     7921 actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt cggttcgatg gaagtgtatg
     7981 aacagcaaac ctcctggctg ctggtattgc agaacagcaa aaccattatg ctgtggggct
     8041 ccgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat gtttatgaat
     8101 attacgagca actaaaagcg aaagtcgccg ccggtgaaat tgaggtcatc agcatcgatc
     8161 cggttgtcac atccacccat gagtatctgg ggcgcgagca tgtgaagcac attgcggtta
     8221 acccgcaaac tgacgtgccg ctgcaactgg cgctggcgta tacgctgtac agtgaaaacc
     8281 tgtacgacaa aaactttctc gctaactact gtgtaggttt tgagcagttc ctgccgtatc
     8341 tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa ctgaccggca
     8401 ttgatgccga aaccattcgt gggctggcgc ggcagatggc ggcgaacaga acgcaaatta
     8461 ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg
     8521 ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggttttggt tttggctggc
     8581 actacaacgg cgcaggcacg ccggggcgta aaggcgttat tctgagtggt ttctccggct
     8641 ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa aggctacagc agcaccattc
     8701 cgattgcccg ttttatcgat gcgatcctcg aaccggggaa agtgatcaac tggaacggta
     8761 aatcggtaaa actgccgcca ctgaaaatgt gtatttttgc cggaactaac ccattccatc
     8821 gccatcagca gatcaaccgc attatttaag gctggcgcaa gctggaaacg gttatcgcca
     8881 tagataacca gtggacctca acctgccgct ttgccgatat cgtactgcct gcgaccacgc
     8941 agtttgagcg taacgatctc gaccagtacg gcaaccactc caaccgtggc attatcgcca
     9001 tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga ctttgatatt ttccgcgagc
     9061 tgtgccgtcg ctttaatcgc gaagaagcct ttaccgaagg gctggacgaa atgggctggc
     9121 tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg acgcggcgtt catctgccag
     9181 cgtttgatga cttctggaat aacaaagagt acgtcgagtt tgaccatccg cagatgtttg
     9241 ttcgccacca ggcattccgc gaagatccgg atctcgaacc gctgggcacg ccgagtggcc
     9301 tgattgagat ctactcgaaa accatcgccg atatgaacta cgacgattgt caggggcatc
     9361 cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc cggctcgcag aagtatccgt
     9421 tgcatctgca atctgtgcat ccggatttcc gacttcactc gcagttatgt gagtcggaaa
     9481 ctctgcgtca gcaatatacg gtagcgggta aagagccagt attcattaac ccgcaggatg
     9541 ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt ctttaacgct cgcggtcagg
     9601 tgttggcagg ggcagtagtt tctgaccgct atgcacccgg cgtggcacga attcacgaag
     9661 gggcatggta cgatccagat aaaggcggcg agcctggtgc gctgtgcaaa tacggtaacc
     9721 ccaacgtgct gaccatcgac atcggtactt cgcagctggc gcaggcgacc agtgcgcaca
     9781 ctacgctggt ggaaattgag aagtacaatg gagcagtgga gcaggtgacg gcgtttaacg
     9841 gccctgtgga gatagtggcg cagtgcgaat atgttcccgc gtcgcaggtg aaatcatgac
     9901 cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg ctggcgcagt tgttctcccg
     9961 tgaactggac gatgaacaac tgacgcaaat cgccagtgcg cagatggcgg aatggttttc
    10021 gttgctgaaa agcgaaccgc cgctggctgc ggcggtgaat gagctggaaa actgtattgc
    10081 cacgctgaca gtacgtgacg atgcccgtct ggaactggcc gctgactttt gcggcctgtt
    10141 tttgatgact gataagcagg cggcgctgcc gtatgcatcg gcctacaaac aggacgagca
    10201 agagattaaa cgcttgttag ttgaggccgg gatggaaacc agcggcaatt tcaacgaacc
    10261 ggcagaccat ctggcgatct atctcgagtt gttgagtcat ctgcattttt cgctgggaga
    10321 ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa aaaacactga cggcgctgtg
    10381 gcaatggtta ccagagtttg ctgcgcgttg ccatcagtat gacagctttg gtttttacgc
    10441 ggcactaagc cagttattgc tggtgttagt ggagtgcgac caccaaaaca aataacgtcg
    10501 tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc attatggcgc agttgccgga
    10561 tgcgtcatca catccggcaa acacacatca cggatgagct acaaaccggg aaagccgctg
    10621 gcgtagcagg cggttttcct gcttcaggtg tgcaatatca tccattaacg tcagcgccac
    10681 cgcgatcccc ggccagtcca gagccagttc atgacgcagg cgtaccgcgc gttgcaccac
    10741 aatggcggca tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg gttcaaccac
    10801 ccccaaaccg acaatttcat tcaactcctc ttcagagatg ccggtatgca ggcaaaattc
    10861 ggtaatagta aaagtcaccg taacattagc catcatgctt tcccccaatc tttacgtgga
    10921 tcaaaagacg actgggcgtc tgccagttgc tgccacagcg cggcagtgtt ttcatccggt
    10981 ttcggcggca tcacgatttt cagtaccgca tacagatcgc cggtctgttt tttgctcacc
    11041 agacctttgc ctttaacgcg caatcgttgc ccggcctggc tgcctggcgg gatagtcagc
    11101 aaaatgcttt ctttcagtgt tggaacggtg actttagcac ccagcgccgc ttcccacggg
    11161 ctaaccggca ccacaatttc cagatcctgg ccgacaatat cgaacagcgg atgtggcgca
    11221 atatgaatca ccagccacaa atcgccattt ggaccaccgt tttcgcccgg cgtcccctgg
    11281 cctttcagac ggatgcgttg accattgccg acgcccgccg ggatcttcac attcagcgtt
    11341 ttcgggattt cctgttcgat catgccaaag gcgttataaa ccggcaggtt atagctgatg
    11401 gtacgcttat gctcagtcag cgtttcttcg aggaataccg ccacttcgat ttcaatatcg
    11461 tggccgcgtg cggcggggcg ttgacggctc tggcgggcat gctgaccgaa aattgacgag
    11521 aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt cgccatgatg gaactgacgg
    11581 ttaaattgcg gatcgttgcg atgttgccac atctgatcat actcagcgcg acgttgttca
    11641 tcacttaaca cttcccaggc ttcagcgacc tctttgaagc gggcttcggc atccggttct
    11701 ttgctgacat caggatggta tttacgggcc agtcgacgat aggcggtctt gattgtcttg
    11761 agatcgtccg tcggtttcac gcccatgatg gcgtaataat cctttaattc catagcgtta
    11821 tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag gtaactccta taagtgtagg
    11881 gtaatcctca aaatttcata tgccaacaca gaatatgtta ttgaaatcat cgtggagagg
    11941 aggtcgccat caatatgggt tgctgaacat attttaaaca ggtgaaaaag ggtgagcgat
    12001 ttttgatagt tgaaccaggc actttaagtt taactagggc gtcatta
//
LOCUS       DAJVOY010000120        11917 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVOY010000120 DAJVOY010000000
VERSION     DAJVOY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.120"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            217..1326
                     /gene="livK"
                     /locus_tag="OQL90_002888"
     CDS             217..1326
                     /gene="livK"
                     /locus_tag="OQL90_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS1369597.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1374..2300
                     /gene="livH"
                     /locus_tag="OQL90_002889"
     CDS             1374..2300
                     /gene="livH"
                     /locus_tag="OQL90_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS1369598.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2297..3574
                     /gene="livM"
                     /locus_tag="OQL90_002890"
     CDS             2297..3574
                     /gene="livM"
                     /locus_tag="OQL90_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS1369599.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3571..4338
                     /gene="livG"
                     /locus_tag="OQL90_002891"
     CDS             3571..4338
                     /gene="livG"
                     /locus_tag="OQL90_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS1369600.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4340..5053
                     /gene="livF"
                     /locus_tag="OQL90_002892"
     CDS             4340..5053
                     /gene="livF"
                     /locus_tag="OQL90_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS1369601.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5450..6121
                     /locus_tag="OQL90_002893"
     CDS             5450..6121
                     /locus_tag="OQL90_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCS1369602.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENRHLPGDRRRHVRRAGSGH
                     HDADL"
     gene            6051..6254
                     /locus_tag="OQL90_002894"
                     /pseudo
     CDS             6051..6254
                     /locus_tag="OQL90_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
     gene            6251..7096
                     /gene="ugpE"
                     /locus_tag="OQL90_002895"
     CDS             6251..7096
                     /gene="ugpE"
                     /locus_tag="OQL90_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS1369603.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATTTF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            7098..8168
                     /gene="ugpC"
                     /locus_tag="OQL90_002896"
     CDS             7098..8168
                     /gene="ugpC"
                     /locus_tag="OQL90_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCS1369604.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            8165..8908
                     /gene="ugpQ"
                     /locus_tag="OQL90_002897"
     CDS             8165..8908
                     /gene="ugpQ"
                     /locus_tag="OQL90_002897"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1369605.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVVGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(8895..9335)
                     /locus_tag="OQL90_002898"
     CDS             complement(8895..9335)
                     /locus_tag="OQL90_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS1369606.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            9455..11188
                     /gene="ggt"
                     /locus_tag="OQL90_002899"
     CDS             9455..11188
                     /gene="ggt"
                     /locus_tag="OQL90_002899"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS1369607.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAQPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(11226..11510)
                     /locus_tag="OQL90_002900"
                     /pseudo
     CDS             complement(11226..11510)
                     /locus_tag="OQL90_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
     gene            complement(11710..11865)
                     /locus_tag="OQL90_002901"
     CDS             complement(11710..11865)
                     /locus_tag="OQL90_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369608.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         2703 a         2955 c         3385 g         2874 t
ORIGIN      
        1 caaaggcact tttttctgtt tatctatcaa taaattcaga atattatctg ttcttaatcg
       61 actgaaagat agggatttta atcactatta tcacaaaata ctgcgctaaa cccttaatca
      121 gacaggcaaa aacagtgcag tataaaaaaa gaacagtctg atttgttaac acataaaaac
      181 aaagcaacac aacatcacga atggggattt ttgactatga aacggaatgc gaaaactatc
      241 atcgcaggga tgattgcact ggcaatttca cacaccgcta tggctgacga tattaaagtc
      301 gccgttgtcg gcgcgatgtc cggcccgatt gcccagtggg gcgatatgga atttaacggc
      361 gcgcgtcagg cgattaaaga cattaatgcc aaagggggaa ttaagggcga caagctggtt
      421 ggcgtggaat atgacgacgc ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc
      481 gttaatgacg gcattaaata cgttattggt catctgtgtt cttcttctac ccaacctgca
      541 tcagatatct acgaagacga aggtattctg atgatctcac cgggagcgac caacccggag
      601 ctgacccaac gcggttatca acacattatg cgtactgccg ggctggactc ttcccagggg
      661 ccaacggcgg caaaatacat tcttgagacg gtgaagcccc agcgcatcgc catcattcac
      721 gacaaacaac agtatggcga agggctggcg cgttcggtgc aggacgggct gaaagcggct
      781 aacgccaacg tcgtcttctt cgacggtatt accgccgggg agaaagattt ctccgcgctg
      841 atcgcccgcc tgaaaaaaga aaacatcgac ttcgtttact acggcggtta ctacccggaa
      901 atggggcaga tgctgcgcca ggcccgttcc gttggcctga aaactcagtt tatggggccg
      961 gaaggtgtgg gtaacgcatc attgtcgaat attgccggtg atgctgccga aggcatgttg
     1021 gtcactatgc caaaacgcta tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg
     1081 aaagcagaca agaaagatcc gtccgggcct tatgtctgga tcacctacgc ggcggtgcaa
     1141 tctctggcga ctgcccttga gcgtactggc agcgatgagc cgctggcgct ggtgaaagat
     1201 ttaaaagcta acggtgcaaa caccgtgatt gggccgctga actgggatga aaaaggcgat
     1261 cttaagggat ttgattttgg tgtcttccag tggcacgccg acggttcatc cacggcagcc
     1321 aagtgatcat cccaccgccc gtaaaatgcg ggcgggttta gaaaggttac cttatgtctg
     1381 agcagttttt gtatttcttg cagcagatgt ttaacggcgt cacgctgggc agtacctacg
     1441 cgctgatagc catcggctac accatggttt acggcattat cggcatgatc aacttcgccc
     1501 acggcgaggt ttatatgatt ggcagctacg tctcatttat gatcatcgcc gcgctgatga
     1561 tgatgggcat tgataccggc tggctgctgg tggcggcggg attcgtcggc gcaatcgtca
     1621 ttgccagcgc ctacggctgg agtatcgaac gggtagccta tcgcccggtg cgtaactcta
     1681 agcgcctgat tgcgctcatc tcggcaatcg gcatgtccat cttcctgcaa aactacgtta
     1741 gcctgactga aggttcgcgt gacgtggcgc tgcccagtct gtttaacggt cagtgggtgg
     1801 tggggcatag cgaaaacttc tctgcctcta ttaccaccat gcaggcagtg atctggattg
     1861 ttactttcct cgccatgctg gcgctgacga ttttcattcg ttattcccgt atggggcgcg
     1921 cctgccgtgc ctgcgcggaa gatctgaaaa tggcgagcct gcttggcatc aacaccgacc
     1981 gggtgattgc tttgaccttt gttattggcg cggcgatggc ggcagtggct ggcgtactgc
     2041 tcggtcagtt ctacggcgtc attaacccct atatcggctt tatggccggg atgaaagcct
     2101 ttaccgctgc ggtgctcggt gggattggca gcattccagg ggcgatgatt ggtgggctga
     2161 tcctggggat tgcggaggcg ctctcttctg cctatctgag tacggaatat aaggatgtgg
     2221 tttcatttgc cctgctgatt ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc
     2281 cggaggtaga gaaagtatga aaccgatgca tattgcaatg gcgctgctct ctgccgcgat
     2341 gttttttgtc ctggcgggcg tctttatggg cgtgcaactg gagctggatg gcaccaaact
     2401 ggtggtcgac acggcttcag acatccgttg gcagtgggtg tttatcggca cggcggtggt
     2461 ctttttcttc cagcttttgc gaccggcttt ccagaaaggg ctaaaaagcg tttccggacc
     2521 gaagtttatt ctgcccgcga ttgatggctc cacggtgaag cagaaactgt tcctcgtggc
     2581 gctgttggtg cttgcggtgg cgtggccgtt tatggtgtca cgtgggacgg tggatatcgc
     2641 cactctgacc atgatctaca ttatcctcgg tctcgggctg aatgtggttg ttggtctttc
     2701 tggtctgctg gtgctggggt acggcggttt ttacgccatc ggcgcttaca cctttgcgct
     2761 gctcaatcac tattacggct tgggcttctg gacttgtctg ccgattgccg ggttaatggc
     2821 agcggcggcg ggcttcctgc tcggctttcc ggtgctgcgt ctgcgcggtg attatctggc
     2881 gatcgttacc ctcggcttcg gcgaaattgt gcgcatattg ctgctcaata acaccgaaat
     2941 taccggcggc ccgaacggaa tcagtcagat cccgaaaccg actttcttcg gccttgagtt
     3001 cagccgtacc gcccgtgaag gcggttggga cacgttcagt aatttctttg gcctgaaata
     3061 cgatccctcc gatcgcgtca tcttcctgta tctggtggcg ttgctgctgg tggtgctaag
     3121 cctgtttgtc attaaccgcc tgctgcggat gccgctgggg cgtgcgtggg aagcgttgcg
     3181 tgaagatgag atcgcctgcc gttcgctggg cttaagcccg cgtcgtatca agctgaccgc
     3241 ttttaccatc agtgccgcgt ttgccggttt tgccggaacg ctgtttgctg cgcgccaggg
     3301 ctttgtcagc ccggaatcct tcacctttgc tgaatcggcg tttgtactgg cgatagtggt
     3361 gctcggcggt atgggatcgc agtttgcggt gattctggcg gcaattttgc tggtggtgtc
     3421 gcgcgagttg atgcgtgatt tcaacgaata cagcatgtta atgctcggtg gtttgatggt
     3481 gctgatgatg atctggcgtc cgcagggctt gctgcccatg acgcgcccgc aactgaagct
     3541 gaaaaacggc gcagcgaaag gagagcaggc atgagtcagc cattattatc tgtgaacggc
     3601 ctgatgatgc gcttcggcgg cctgctggcg gtgaacaacg tcaatcttga actgtacccg
     3661 caggagatcg tctcgttaat cggtcctaac ggtgccggaa aaaccacggt ctttaactgc
     3721 ctgaccggat tctacaaacc cactggaggc accattttac tgcgcgatca gcatctggaa
     3781 ggtttaccgg ggcagcaaat tgcccgcatg ggcgtggtgc gtaccttcca gcatgtgcgt
     3841 ctgttccgtg aaatgacggt gattgaaaac ctgctggtgg cgcagcatca gcaactgaaa
     3901 accgggctgt tctctggcct gttgaaaacg ccatccttcc gtcgcgccca gagcgaagcg
     3961 ctcgatcgcg ccgcgacctg gcttgagcgc attggtttgc tggaacacgc caaccgtcag
     4021 gcgagtaacc tggcctatgg tgaccagcgc cgtcttgaga ttgcccgctg catggtgacg
     4081 cagccggaga ttttaatgct cgacgaacct gcggcaggtc ttaacccgaa agagacgaaa
     4141 gagctggatg agctgattgc cgaactgcgc aatcatcaca acaccactat cttgttgatt
     4201 gaacacgata tgaagctggt gatgggaatt tcggaccgaa tttacgtggt caatcagggg
     4261 acgccactgg caaacggtac gccggagcaa atccgtaata acccggacgt gatccgtgcc
     4321 tatttaggtg aggcataaga tggaaaaagt catgttgtcc tttgacaaag tcagcgccca
     4381 ctacggcaaa atccaggcac tgcatgaggt gagcctgcat atcaatcagg gcgagattgt
     4441 cacgctgatt ggcgcgaacg gggcggggaa aaccaccttg ctcggcacgt tatgcggcga
     4501 tccgcgcgcc gccagcgggc gaattgtgtt tgatgataaa gacattaccg actggcagac
     4561 ggcgaaaatc atgcgcgaag cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg
     4621 gatgacggtg gaagagaacc tggcgatggg cggctttttt gccgaacgcg accagttcca
     4681 ggagcgcata aagtgggtgt atgaactgtt tccacgtctg catgagcgcc gtgttcagcg
     4741 ggcaggcacc atgtccggcg gtgagcaaca aatgctggcg attggtcgcg cgctgatgag
     4801 caacccgcgt ttgctgctac ttgatgagcc atcgctcggt cttgcgccga ttatcatcca
     4861 gcaaattttc gacaccatcg agcagctgcg cgagcagggg atgactatct tcctcgtcga
     4921 gcagaacgcc aaccaggcgc taaagctggc ggatcgcggc tacgtgctgg aaaacggcca
     4981 tgtggtgctc tccgataccg gcgatgcgct gctggcgaac gaagcagtga gaagtgcgta
     5041 tttaggcggg taataacacg ttgattgata aggagtcaaa agactccttt gagacaggtg
     5101 acaaatgtaa aattgcctga tgcgctgcgc ttatcaggcc tactgggtga gttgcaatat
     5161 attgaatttg cacgatcttg taggccggat aaggcgttta cgccgcatcc ggcatgaaac
     5221 gatgagtaat ctgtagagtt tgattcagac cttctatttt tccgcttatc cgtgccccat
     5281 ctcccatttt ccctcaccca cgccgtcacc gccttgtcat ctttctgaca ccttactatc
     5341 ttacaaatgt aacaaaaaag ttatttttct gtaattcgag catgtcatgt taccccgcga
     5401 gcataaaacg cgtgaattcg cgcattcggt acaacaagag agataaacga tgaaaccgtt
     5461 acattataca gcttcagcac tggcgctcgg actggcgtta atggggaatg cacaggcagt
     5521 gacgaccatt ccgttctggc attctatgga aggggaactg ggtaaagagg tggattctct
     5581 ggcccaacgt tttaacgccg aaaatccgga ttacaaaatt gtaccgacct ataaaggcaa
     5641 ctacgaacag aatttaagcg cggggattgc cgcatttcgt accggcaacg ctccggctat
     5701 tttgcaggtt tatgaagttg gcaccgccac catgatggca tcgaaagcca ttaaaccggt
     5761 atatgacgtg tttaaagagg cggggattca attcgatgag tcgcagtttg tgccgacggt
     5821 ttccggttac tactccgaca gcaaaacggg ccacttactc tcccagccat tcaacagctc
     5881 gactcccgtt ctctattaca acaaagacgc cttcaagaaa gcggggttag acccggaaca
     5941 gccgccgaaa acctggcagg atctggcgga ctatgccgcg aaactgaaag cctccggcat
     6001 gaagtgcggc tacgccagcg gctggcaggg ctggatccaa ctggaaaatc gacaccttcc
     6061 cggtgatcga cgccgccacg tccggcgggc cggttcaggc caccacgacg ctgatttata
     6121 agatctaccg cgaaggtttt accggactgg atctggcttc gtctgccgca cagtcggtgg
     6181 tgttgatgtt cctcgtcatc gtgctgacgg tggtgcagtt ccgctatgtt gaaagcaagg
     6241 tgcgttacca atgattgaga accgtccgtg gctgacgata ttcagccata ccatgctgat
     6301 cctcgggatc gcggtgattc tcttcccgct gtacgtggcg tttgtcgcgg cgacgctgga
     6361 taaacaggcc gtctatgccg cgccgatgac gctcatcccc ggcacacatc tgctggaaaa
     6421 tatccacaac atctgggtta acggggtagg tacgaatagc gcgccgttct ggcggatgtt
     6481 gcttaacagc tttgtgatgg cgttcagcat tacgctcggc aaaattaccg tctcgatgct
     6541 ctcggcattt gccattgtct ggtttcgttt tccgctacgt aacctcttct tctggatgat
     6601 ttttatcacc ctgatgctgc cggttgaagt acgtatcttc ccgacggtgg aagtcatcgc
     6661 caacctgaag atgctcgaca gctacgccgg tttaacgctg ccgctgatgg cctcggcgac
     6721 caccacgttt ctgtttcgcc agttctttat gacgctgccg gatgaactgg tggaagcggc
     6781 gcggatcgac ggcgcatcgc caatgcgctt cttttgcgac atcgtttttc cgctctccaa
     6841 aactaatctg gcggcgctgt ttgtgatcac ctttatttac ggctggaacc agtatttgtg
     6901 gccgttgttg attattaccg acgtggatct cggcaccacc gtggcaggga tcaaagggat
     6961 gatcgccact ggcgaaggca ccacggaatg gaactcagtg atggcggcga tgttgttaac
     7021 cctgatccca ccagtagtga ttgttttagt gatgcagcgt gccttcgtgc gcggcctggt
     7081 cgatagtgag aaataagatg gcaggactga aattacaggc agtaaccaaa agctgggatg
     7141 gcaaaaccca ggtcattaaa ccgctgaccc ttgatgtggc tgatggcgaa tttatcgtga
     7201 tggtcgggcc gtctggctgc gggaaatcga cgctgctgcg tatggttgcc gggctggagc
     7261 gggtgaccga aggcgatatc tggatcaacg accagcgcgt gactgaaatg gagccgaaag
     7321 atcgcgggat tgcgatggtg ttccagaact acgcgcttta tccgcatatg agcgtcgaag
     7381 aaaacatggc gtgggggctt aaaattcgcg gcatgggcaa gcagcaaatt gccgagcgcg
     7441 ttaaagaagc ggcgcgcatt ctggagctgg acggtctgct caaacgtcgc ccgcgtgagc
     7501 tatccggcgg tcagcgtcag cgtgtagcga tgggccgcgc gattgtgcgc gatccggcgg
     7561 tgttcctgtt tgatgagccg ctctctaacc tcgatgccaa gctgcgcgtg cagatgcgtc
     7621 ttgaactgca acagttgcac cgtcgcctga aaacgacttc actctacgtt actcacgatc
     7681 aggttgaagc gatgacgctc gcccagcgag taatggtgat gaacggcggc gttgccgaac
     7741 agattggcac accagttgaa gtctacgaaa agcccgccag cctgtttgta gcgagtttta
     7801 tcggcagtcc ggcgatgaac ctgctgacag gccgcgtgaa taacgaaggc acgcatttcg
     7861 aactggacgg cggtattgag ctgccgctaa acggtggcta ccgtcagtat gccgggcgta
     7921 aaatgactct cggcattcgc ccggaacata ttgtgctaag ctcgcaggca gaaggcggcg
     7981 taccgctggt gatggacacg ctggagatcc tcggcgcaga taacctggcg cacggacgct
     8041 ggggcgagca gaagctggtg gtgcgactgg cgcatcagga gcgcccgacg gcaggcagca
     8101 cgctgtggct gcatctggcg gaaaatcagc tgcatctttt tgatggtgaa acaggacaac
     8161 gagtatgagt aactggcctt atccccgcat cgtcgctcat cgtggcggcg gtaagctggc
     8221 cccggaaaac accctggcgg caatcgacgt cggggcaaaa tacggtcata agatgatcga
     8281 atttgacgcg aagttatcga aagatggcga gatcttcctg ctccatgacg acaatctcga
     8341 acgcaccagc aacggctggg gcgtcgtggg tgaactgaac tggcaggatt tactgcgcgt
     8401 ggatgcgggc agttggtaca gcaaagcgtt taaaggtgag ccgctgccgt tgctttcgca
     8461 ggtggcggaa cgctgtcgcg aacatgggat gatggcgaat atcgaaatca aacccaccac
     8521 tggcactgga ccattaacgg gcaaaatggt ggcgctggcg gcacgccaac tgtgggccgg
     8581 tatgacgccg ccgctgctgt catcgtttga gattgatgct ttagaagctg cacagctggc
     8641 ggcaccggaa ctgccgcgtg gtttattgct tgatgagtgg cgcgacgact ggcgcgaact
     8701 gaccgcgcgg ctgggctgcg tctctattca tctcaatcat aagttactcg ataaagcgcg
     8761 agtgatgcag ttgaaagacg ccggattacg gattctggtt tataccgtca ataaacccca
     8821 gcgcgcggca gagttgctgc gctggggcgt ggattgcatc tgtaccgatg cgattgacgt
     8881 gattggtccg aactttacgg cccaatagtt ttcaacggaa tgtcaggctg cggcgtgctg
     8941 ggctgattca gcatgtcgcc gtttctcctc tccggcagca tatgctgctg attcaacgaa
     9001 ctatccggat tacggttgct gtttaacatc ccgccgttgg tgttgggcag catttgctgc
     9061 cgggcgggat ttcgctcccc cggctgtgac tgcaacaccc gctgagaatt attgtttatc
     9121 tggttttcta aatgctgctg ttgcaactga gtttgcgttt tcagttgctg attcagcatc
     9181 cctttttgct ggatttgctg agtttgcatc tgggtttgca tccgctgctg gctgggtatc
     9241 tgataccccg gctggttagg gttgttcaga gtattaatgg gctgtgcaaa gccgacaaac
     9301 ggcaggagtg ccgtaagaat cagaagtcgt ttcatcgcgt atcctcctct gaagatatcc
     9361 tttaagttta ctcgcttccc gacaaaacga tgattaattc agagttatat accaggctta
     9421 gctggggttg ccccttaatc tctggagaat aacgatgata aaaccgacgt ttttacgccg
     9481 ggtagccatt gctgctctgc tctcaggaag ttgttttagc gccgtcgccg cgcctcctgt
     9541 ttcttacggc gtggaggaag atatcttcca tccggtacgc gcgaaacagg ggatggtggc
     9601 gtctgtcgat gccactgcca cccaagtggg ggtagatatt ctaaaggagg gcgggaatgc
     9661 cgttgatgcc gccgtggcgg tgggctacgc gctggcggta acgcatccgc aggcagggaa
     9721 tctgggcggt ggtggtttta tgctgatccg ctcgaaaaat ggcaatacca ccgctatcga
     9781 tttccgcgag atggcacccg ctaaagcgac ccgcgatatg ttccttgatg accagggcaa
     9841 cccggacagc aaaaaatcac tcacttcgca tctggcttcc ggcacgcccg gtacggtcgc
     9901 tggtttctcg ctggcgttgg ataagtacgg cactatgccg ttgaacaaag tggtgcagcc
     9961 cgcgttcaaa ctggcacgcg atggttttat cgttaacgat gctttggcag acgatctcaa
    10021 aacctacggt agcgaagtgt tgccgaatca cgaaaacagc aaagctatct tctggaaaga
    10081 gggcgaaccg ctgaaaaagg gcgacaagct ggtgcaggcg aacctggcaa agagcctgga
    10141 gatgattgcc gaaaacggcc cggacgaatt ctataaaggc acgatagcgg aacagattgc
    10201 ccaggagatg cagaaaaacg gtggcttgat cactaaagaa gatttagcgg cgtataaagc
    10261 ggtcgaacgc acaccgataa gtggcgatta tcgcgggtat caggtttact ccatgccacc
    10321 gccatcctcc ggcgggatcc atatcgtaca aatcctcaat attctggaaa acttcgatat
    10381 gaagaaatac ggctttggca gcgccgatgc gatgcaaatc atggcagaag cggagaaata
    10441 cgcctacgcc gaccgctcgg aatatcttgg cgacccggat tttgtcaaag taccgtggca
    10501 ggcgctgacc aataaagcct atgccaaatc tattgccgat caaattgata tcaataaagc
    10561 gcagccatcc agcgaaattc gccccggcaa gcttgcgcct tatgagagta atcaaactac
    10621 ccattactca gtggtggata aagatggtaa cgcggtggcg gtgacctata cgctgaacac
    10681 caccttcggt acgggcattg tcgcgggcga gagcggtatt ctgcttaata atcagatgga
    10741 tgatttctcc gctaaaccgg gcgtaccgaa cgtttacggg ctggtgggcg gtgatgccaa
    10801 cgccgtcggg ccgaataaac gtccgctgtc gtcaatgtca ccaacgattg tggtgaaaga
    10861 cggtaaaacc tggctggtca ccggtagccc tggcgggagc cggatcatca ctacagtgct
    10921 gcaaatggtg gtgaatagca tcgattatgg catgaacgtc gccgaagcga ccaatgcgcc
    10981 gcgtttccac catcagtggt tgccggacga gctgcgtgtc gaaaaagggt ttagcccgga
    11041 tacgctcaag ctgctggaag caaaaggtca gaaagtggcg ctgaaagagg cgatgggcag
    11101 tacacaaagc attatggttg ggccggacgg tgagttgtac ggcgcatccg acccgcgctc
    11161 ggtggatgat ttaacggcgg ggtactaagg ttagcggccc tcttcgtggg aagagggcta
    11221 ttttgtcagg gcaagccgaa ggtagccttt tttatttcgt aatcctgtag atattcttcc
    11281 agcggctttg ccgttcccag agaatgaatt tcagcactgt ctttatcaat aataaaaggt
    11341 gcgttaccag ctaagcgcgc ggcctcatct ccagtttcga gaaattctcg tgcttcgaaa
    11401 cagaaatacc agccctagct aaagcgtcca tgtagagtaa tgacgaccgg gagatctgca
    11461 tcatcaaggt aatggttcgc tttcgcgaat gcgtcgtgat aagtaatcat aattataata
    11521 aatattattg ttgagtgtta tattattatt cctgtgtgat acattgagca aagacgcgtt
    11581 catcttcgtc aaagatatta ttaaatgctg gttttgaaaa aagttcatcg gataagatat
    11641 agtcacgaaa taagtatctg ctaatatttt atgatttctt tttcagaacg tcgaacggga
    11701 ttttcttatt taagatatca gaatcttatg attcgggtaa aattctaaat aaatcaacat
    11761 gtcattaaaa tcatcatagc cattagctat ttcggctaaa atagagacta catgtcttcg
    11821 gtccatctca cttaaggagt gtagttccgt tgtaagtttt tccatagctt gcactgctaa
    11881 atttcgaaca aggaattttc tgctggtaat ctctaaa
//
LOCUS       DAJVOY010000121        11873 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVOY010000121 DAJVOY010000000
VERSION     DAJVOY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.121"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(39..353)
                     /locus_tag="OQL90_002902"
                     /pseudo
     CDS             complement(39..353)
                     /locus_tag="OQL90_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(468..866)
                     /locus_tag="OQL90_002903"
     CDS             complement(468..866)
                     /locus_tag="OQL90_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HCS1369609.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="OQL90_002904"
     CDS             complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="OQL90_002904"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1369610.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3549..4622)
                     /gene="recF"
                     /locus_tag="OQL90_002905"
     CDS             complement(3549..4622)
                     /gene="recF"
                     /locus_tag="OQL90_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1369611.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="OQL90_002906"
     CDS             complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="OQL90_002906"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1369612.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="OQL90_002907"
     CDS             complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="OQL90_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1369613.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7427..7507
                     /locus_tag="OQL90_002908"
     CDS             7427..7507
                     /locus_tag="OQL90_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369614.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7737..7877
                     /gene="rpmH"
                     /locus_tag="OQL90_002909"
     CDS             7737..7877
                     /gene="rpmH"
                     /locus_tag="OQL90_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1369615.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7894..8253
                     /gene="rnpA"
                     /locus_tag="OQL90_002910"
     CDS             7894..8253
                     /gene="rnpA"
                     /locus_tag="OQL90_002910"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1369616.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8217..8474
                     /gene="yidD"
                     /locus_tag="OQL90_002911"
     CDS             8217..8474
                     /gene="yidD"
                     /locus_tag="OQL90_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1369617.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8477..10123
                     /gene="yidC"
                     /locus_tag="OQL90_002912"
     CDS             8477..10123
                     /gene="yidC"
                     /locus_tag="OQL90_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1369618.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10229..11593
                     /gene="mnmE"
                     /locus_tag="OQL90_002913"
     CDS             10229..11593
                     /gene="mnmE"
                     /locus_tag="OQL90_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1369619.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11836..>11873
                     /locus_tag="OQL90_002914"
     CDS             11836..>11873
                     /locus_tag="OQL90_002914"
                     /inference="COORDINATES: protein motif:HMM:NF019665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCS1369620.1"
                     /translation="MNILHICVTSKW"
BASE COUNT         2714 a         3250 c         3067 g         2842 t
ORIGIN      
        1 ggacaggggt atcgcttagt ttttcaattt cctatacgtg aaccgacttc gcgagagagt
       61 ttttccagga aacgatagtc gtcataactg agagcagttt gcgccacggt gctaccatca
      121 gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
      181 tgcagctctt tcaggtagtt gtgaacgcct gcataagggc gacccgtcgt tagcacgaca
      241 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
      301 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
      361 ccaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
      421 ccgagggaaa agggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt
      481 ttccctttca gcagttccat acctgcggaa agcagatcgt tattggcttg tgaagacact
      541 tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt gttctgccag taaactggaa
      601 gccgtactgg tatccacgcc aagtttttgc ccgagatcgg acaccgcatt tgtgccgggc
      661 gccgattcca gttgctcgcc gctaactgat tgattgctct gctgattact caaccaggtt
      721 gagagaatgg cccctaagcc gccactttgc agtttttcca gcagcacctg aatgccgccc
      781 tgctcctcaa cccaacttaa aatagcctga tatttccccg catcgccttt cagaaaggca
      841 ccgacaactt catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgaccca
      901 aaaagtatag atctgcggga tgataattgc ggattgcaga aataaaaagg gcggagatga
      961 tctccgccct ttttttatag cttcttgccg gatgcggcgt taacgtctta cctggcctac
     1021 aaaatcatta aaattcaata cattgcaaga ttttcgtagg cctgataagc gtagcgcatc
     1081 aggcacgctc gcatggttaa cgccattaaa tatcaatatt cgccgctttc agggcgttct
     1141 cttcaataaa cgcacgacgc ggttctacgg cgtcgcccat cagcgtggtg aacaacaggt
     1201 cagcggcaat cgcatcctta acggtaacgc gcagcatacg acgactttcc gggtccatgg
     1261 tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga
     1321 gaccgcgacg agactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct
     1381 gacgacgctc gccacgttcg ataaatgcat cttcttccag caagccacgc agtttctcac
     1441 ccagcgtgca gatacgacga tattcgccac cagtgataaa ttcgtgatcc agcggatagt
     1501 cagtatccac accgtgggta cgcacgcgaa caatcggctc gaacaggttt tgttcggcat
     1561 tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga
     1621 ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga gaggtcggct tccgtcagcg
     1681 tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga
     1741 tcattttctg cgtcgcgttg tactcagaca ccagtttctc taacgcttcg ccagccaatg
     1801 ccggtgcgct ggcgttggtg tgcagcgttg cgccgtccag cgcgatagag atctggtact
     1861 gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca
     1921 gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga
     1981 agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga
     2041 tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa
     2101 gcgcggtgat aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct
     2161 caacgttgag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct
     2221 gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat
     2281 cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc gcacctttac
     2341 ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt
     2401 tgccaaccac gattttcgcg tcggttgggt tttccagcag gtattctgcc agcagttcgt
     2461 tcatctgctg ttcaaccgcc gatttcacct cagaagaaac cagtttgtct ttggtctggg
     2521 aggagaattt cgggtccggc acttttacgg aaacgaccgc aatcaggcct tcacgcgcat
     2581 cgtcaccggt ggcgctgact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcgt
     2641 tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg
     2701 gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc atcgttccac tgcaacgcca
     2761 cttcgacgcc aataccgtct ttttcggtgg agaagtagaa gatattcgga tggatcggcg
     2821 ttttgttctt gttcagatat tcaacgaacg ccttgatgcc gccttcatag tggaagtggt
     2881 cttctttgcc gtcacgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca
     2941 actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt
     3001 cgaggctggg ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca
     3061 gcggagcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgga
     3121 taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac
     3181 cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga
     3241 tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt
     3301 catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac
     3361 ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg
     3421 tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt
     3481 tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct cgctcattta
     3541 tacttgggtt aatccgttat tttacccttt tccacggtaa acatcttcga attttcgtcc
     3601 gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct
     3661 tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct
     3721 atcaggtaga gacaccgccg cccgctttca cgggtgagga actctccttg cgccagacgt
     3781 aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg
     3841 tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga
     3901 tcgcgttcaa aattacgttc cagcacctca gcatattctg tctctttctc ccagccgcgc
     3961 tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaagtatc ggccatatca
     4021 gccgcgatac cggcgctata ctccgcgcgc caggtgctga tttgctccac cagcgggatc
     4081 agctctttat cccacgggcg tagctgttcg taacgtgtca cctggcgcag cgccgcattg
     4141 cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccggg ttcgttgtga
     4201 aagcatgccc agtcgagaaa tgctcttctg tatttggggc cgccgttgag taaagtaaac
     4261 ccttctggcg ttatcagctg catcggcatc aggtgcgcca gctccgcgac tttatgcccg
     4321 tcagtaccgt cgatgcggac tttgctgtcg ccttgtttgt ctttggttaa gccaatcgcc
     4381 gtctcgcgct cttcgccctg taatcgccca tggagaacaa atgcctcctg ctcatggcga
     4441 atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct
     4501 tccagcacac tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat
     4561 aaggcgagat ccgcggtttc gatattgcgg aaatcgcgga tcaacaagcg ggtgagggac
     4621 attacagtct cattggcatg acaacataag ccgcgctctg gctggccgca tcttcaatct
     4681 gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca
     4741 gaacatccag cacatagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa
     4801 cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt ggcagtgatt ttcagctggt
     4861 tttcgctgac ataaagacgt acgccgcgga atttctcgtt agagagaatt gccgcacggg
     4921 caaacgcctg cttgagcaga tcgcagccag cttccagatg tttgtccggg ttcttcggta
     4981 gaacgcggcg gtaatccggg aagcgaccat ccaccagttt ggaggtgaag ataaagtcgc
     5041 caacgtgggc acgaatgtta ttgctgccaa tctgcacgcg cagcggattg tcgccgccgt
     5101 cgagcatacg catcagttca atcacgcctt tacgcggcac gatcaccgaa tggcttggca
     5161 aagattgacc aattggcatt gaacagactg ccagacggtg accatcggtc gctacggtgc
     5221 gcagttcttc gccttcggtt tcaaacagca taccgtttaa gtaatagcga acatcctgat
     5281 gcgccatcga aaactgggtc gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa
     5341 attcgacttc gctctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca
     5401 gcgaaaaacg gctacgcccg gagcgcacca gcatccgttc accttccagc tgcacggcaa
     5461 tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg
     5521 ttgctcctgg ctcgtgtggc tgaaccagcg caacacgtgc caccatttcc atctcgagat
     5581 cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg
     5641 gtagcgtagg acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct
     5701 cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa
     5761 tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc
     5821 accgtcgtgt ggtcacgacc accaaacgca tcgccaatct ccggcagact gtggttagtc
     5881 agctctttcg ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc
     5941 ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg
     6001 tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag
     6061 tcgatggtga tcgcccgtcc ggtaaagttg gcattggcaa tgacgcggtt cagcgcccct
     6121 tccagctcac gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcgcccggc
     6181 aaacgaatgt cgttttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct
     6241 ggcggttcga tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg
     6301 ccgttgatct ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc
     6361 agggcgttga aggtatggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga
     6421 atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg
     6481 ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact
     6541 ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagtag gtgagtttta
     6601 cccagacccg tgccgccata aaggaacaac gggttatagg caccgccagg gttatccgcc
     6661 acctggcgag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac
     6721 gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc
     6781 caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg
     6841 gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact
     6901 tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta
     6961 ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggccagc
     7021 gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt
     7081 aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc
     7141 actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg
     7201 aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag
     7261 atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt
     7321 tctcacagat tttcccgatt tatccacagg actttccaga actcgctaag tgtaaacgat
     7381 cctgccgcga ggcgggcacg atttacgcca catattgaaa aatttaatga ccatagacaa
     7441 aaattggctt aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc
     7501 cgtataatcc tccacccggc gcgccatgct ggtttccact ggtgtgaggt cgtacatttt
     7561 ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggagtgt
     7621 acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt
     7681 ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga
     7741 aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta
     7801 tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc
     7861 tgaccgtttc taagtaatca agctaacccc tgagtggtta agctcgcatt tcccagggag
     7921 ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc
     7981 acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt
     8041 acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgtctgacg
     8101 cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg
     8161 aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg
     8221 cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc
     8281 tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa
     8341 ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat
     8401 taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata
     8461 ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt
     8521 gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca
     8581 gaccacgcag acaacgacca ccgcagcggg tagcgccgcc gaccagggcg taccggccag
     8641 tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg
     8701 tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca
     8761 gccgttccag ctgctggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac
     8821 cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa
     8881 agacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga
     8941 cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa
     9001 cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcca cctttggtca
     9061 gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact
     9121 gcacaccttc cgcggcgcgg cgtactccac gcctgacgag aagtacgaga aatacaagtt
     9181 cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcaat
     9241 gctgcaacag tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta
     9301 taccgctaat ctgggtaacg gcatcgccgc tatcggctat aaatctcagc cggtactggt
     9361 tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga
     9421 caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt
     9481 catctctcag ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg
     9541 cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaaac
     9601 gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga
     9661 gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg atggcgctgt acaaagctga
     9721 gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc
     9781 gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat
     9841 ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat
     9901 gttcttcatt cagaagatgt cgccgaccac agtgaccgac ccgatgcagc agaagatcat
     9961 gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtgct
    10021 gtactatatc gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct
    10081 ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct
    10141 aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggcggata agcaccgcgc
    10201 atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc
    10261 cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcttca aagcccgtga
    10321 agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt
    10381 taaagacgcc gacggcagcg tactcgatca ggggattgcg ctatggttcc ctggcccgaa
    10441 ctcgttcacc ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg tgatcctcga
    10501 cctgctgtta aaacgtattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt
    10561 ttccgaacgc gcgtttctta acgataaact tgacttagcc caggccgagg cgattgccga
    10621 tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc
    10681 attctccgca cgggttaatc atctggtaga agccctcacc cacttgcgca tttacgtcga
    10741 agcggcaatt gatttccccg atgaagagat cgatttcctc tccgacggaa aaattgaagc
    10801 ccagctcaat gacgttattg ccgatcttga tgcagtgcgt gctgaagcac gtcagggtag
    10861 tttgttgcgc gaagggatga aagtggtgat tgccggacgt cctaacgccg gtaaatcgag
    10921 cctgttaaac gcgctggcgg ggcgtgaagc ggcaatcgta accgatatcg ccggaactac
    10981 gcgtgacgtg ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgatac
    11041 cgccgggcta cgtgaagcca gtgacgaagt agaacgtatt ggtatcgagc gcgcgtggca
    11101 ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat ggcaccacaa cagacgccgt
    11161 ggatccggca gagatctggc cggaatttat tgcccgtctg ccagcgaaac tgccgatcac
    11221 cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg
    11281 tcacgcgtta attcgtctct cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca
    11341 tctcaaacag agcatgggct ttgacaccaa catggaaggc ggcttcctgg cgcgtcgtcg
    11401 ccacctacag gcgctggaac aggcagcgga acatctacaa cagggcaaag cgcaactgtt
    11461 gggagcctgg gcaggtgaac tgctggcgga agagttgcgt ctggcacagc agaacttaag
    11521 cgaaatcacc ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg
    11581 tattggtaag taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc
    11641 attgttagca ctcctgttat tcctcaaccc tttttttaaa cattaaaatt cttacgtaat
    11701 ttataatctt aaaaaaagca tttaatattg ctccccgaac gattgtgatt cgattcacat
    11761 ttaaacaatt ttagaataga caaaattctt gagtattata ctgtagcctc gtgtctttcg
    11821 aggaataagt gcattatgaa tatcttacat atatgtgtga cctcaaaatg gtt
//
LOCUS       DAJVOY010000122        11833 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVOY010000122 DAJVOY010000000
VERSION     DAJVOY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.122"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..279)
                     /locus_tag="OQL90_002915"
     CDS             complement(<1..279)
                     /locus_tag="OQL90_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1369621.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAK"
     gene            435..1475
                     /gene="gpr"
                     /locus_tag="OQL90_002916"
     CDS             435..1475
                     /gene="gpr"
                     /locus_tag="OQL90_002916"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1369622.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1515..2009)
                     /locus_tag="OQL90_002917"
     CDS             complement(1515..2009)
                     /locus_tag="OQL90_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1369623.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2200..3084
                     /gene="yghA"
                     /locus_tag="OQL90_002918"
     CDS             2200..3084
                     /gene="yghA"
                     /locus_tag="OQL90_002918"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1369624.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3356..3781)
                     /gene="exbD"
                     /locus_tag="OQL90_002919"
     CDS             complement(3356..3781)
                     /gene="exbD"
                     /locus_tag="OQL90_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1369625.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDLVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3788..4522)
                     /gene="exbB"
                     /locus_tag="OQL90_002920"
     CDS             complement(3788..4522)
                     /gene="exbB"
                     /locus_tag="OQL90_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1369626.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4774..5961
                     /gene="metC"
                     /locus_tag="OQL90_002921"
     CDS             4774..5961
                     /gene="metC"
                     /locus_tag="OQL90_002921"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1369627.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6101..6760
                     /locus_tag="OQL90_002922"
     CDS             6101..6760
                     /locus_tag="OQL90_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1369628.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6800..7699)
                     /gene="yqhC"
                     /locus_tag="OQL90_002923"
     CDS             complement(6800..7699)
                     /gene="yqhC"
                     /locus_tag="OQL90_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1369629.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            7893..9056
                     /gene="yqhD"
                     /locus_tag="OQL90_002924"
     CDS             7893..9056
                     /gene="yqhD"
                     /locus_tag="OQL90_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1369630.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9161..9988
                     /gene="dkgA"
                     /locus_tag="OQL90_002925"
     CDS             9161..9988
                     /gene="dkgA"
                     /locus_tag="OQL90_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1369631.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            10188..11114
                     /locus_tag="OQL90_002926"
     CDS             10188..11114
                     /locus_tag="OQL90_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1369632.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11165..11422
                     /gene="yqhH"
                     /locus_tag="OQL90_002927"
     CDS             11165..11422
                     /gene="yqhH"
                     /locus_tag="OQL90_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1369633.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(11457..11765)
                     /locus_tag="OQL90_002928"
     CDS             complement(11457..11765)
                     /locus_tag="OQL90_002928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369634.1"
                     /translation="MDYFCRNESVIDTATQKIIIQPGCHFARSSAHITSQIETEFIGY
                     WYIVITGQWLVQTFYRFINRFFAQHVSAEATEQQNKKTGVPASHNHVLMFGFFLANSL
                     "
BASE COUNT         3069 a         2994 c         2953 g         2817 t
ORIGIN      
        1 ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg aaggatacgt
       61 gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgtca gcacataatt
      121 tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat acttcgccgc
      181 aaggttgagg aactcacgtt tcgaaatttt cccgtgagcg taatagtcgt agtaatccaa
      241 caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc tcaactggtg
      301 ttttttaagc aaagcataag cgcgtgtttt tgcccagttt ttcgtcactc tgtgagccag
      361 actacgggat acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa attgaacggt
      421 ggaaaggaga ggtcatggtc tggttagcga atcccgaacg ttacgggcag atgcagtacc
      481 gctattgcgg aaaaagtggt ttacgcctgc ccgcgttatc gctcggttta tggcacaatt
      541 tcggtcacgt taacgcgctg gaatcacagc gtgcaatcct gcgtaaagcg tttgatttag
      601 gcattacgca ctttgattta gccaacaatt acgggccgcc tccaggaagc gcagaagaga
      661 actttggtcg cctgctgcgg gaggattttg ccgcttatcg cgatgaactg attatctcta
      721 ccaaggctgg ctacgatatg tggcccggcc cttacggctc tggcggttca cgtaaatacc
      781 tgctcgccag cctcgaccaa agcctgaagc gtatggggct tgagtatgtc gatatctttt
      841 actctcatcg cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg ctggctcatg
      901 cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca gagcggacgc
      961 aaaaaatggt cgagttgctg cacgagtgga aaattccgct tttaattcat caaccttcgt
     1021 acaatttact gaaccgctgg gtggataaaa gcggcctgct ggataccctg caaaataacg
     1081 gcgtgggctg tattgccttt actcctctgg ctcagggatt gctgaccgga aaatatctca
     1141 acggcattcc gcaagattca cggatgcatc gtgaagggaa taaagttcgt ggtctgacgc
     1201 cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt attgaatgaa atggcacagc
     1261 agcgtggaca atcaatggcg caaatggcgt taagctggtt gctgaaagat gatcgcgtga
     1321 cgtcggtatt gattggtgtc agccgcgcgg agcaactaga ggagaacgtg caggcgctga
     1381 ataatctgac atttagcacc gaggagctgg cgcagattga tcagcatatc gccgatggcg
     1441 agctgaatct gtggcaggcg tcttccgata aatgacctgt taataacggg cgcgcgaacc
     1501 gcgcccgaat cagatcagtg attatgacga gtcagtcggt caagatagcc catcacaaat
     1561 gcagagagca caaacgtcag atggataatg acgtaccaca tcagtttgtt atccgggaca
     1621 tttttcgcat ccataaagac gcgcagtaag tgaatggaag aaattgccac aatcgacgct
     1681 gctactttgt ttttcagcga cgttgcgtcc attttcccca gccagttcag cttctctttg
     1741 ttctcggaga tatccagctg cgagacgaaa ttctcataac cggaaaacat caccatcacc
     1801 agcaaaccgc caaccagcgt catatccacc agcgacagca acacgaggat caaatctgat
     1861 tccgccatcg agaagatatt cggcagtacg tgaataatct cctggaagaa cttcagcgcc
     1921 agggcaacta acgccagcga aaggccaaag tacacggggg caagcagcca gcgagaagca
     1981 tacattgcat tttcaagaaa acgttccata cattcctgtc gtgtagttaa aacgggcaac
     2041 agtatatcgc aattgagtaa acagcacgca acagcaaatc aacatatacg ttaaaccttt
     2101 gtcgcataat tctgaggttt agggatcttc ctggttccct tatcaacgca tcgagagcgt
     2161 ctacccttaa aagacgttga acctgaaggg agaaaaacaa tgtctcattt aaaagacccg
     2221 actacgcagt attacactgg tgaatatccc aaacagaaac aaccgacgcc aggcatccag
     2281 gcgaagatga caccggtacc ggattgcggc gagaaaacct atgttggtag cggtcgcctg
     2341 aaagatcgta aagcactggt gacagggggc gattccggaa taggtcgcgc tgccgccatc
     2401 gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga agaagaagac
     2461 gctcaggatg tgaaaaagat cattgaagaa tgcggacgca aagccgttct gctgccaggc
     2521 gatttaagcg atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa ggcgttaggc
     2581 gggctggata ttatggcgct ggtcgccggg aaacaggttg ccattccgga tattgcagac
     2641 ctcaccagcg aacagtttca aaagaccttt gccattaacg ttttcgcgct gttctggcta
     2701 acccaggaag cgatccccct gctaccgaaa ggtgcaagta tcatcaccac ttcgtcaatc
     2761 caggcatacc agccaagtcc gcatttactg gactatgcgg ctacgaaggc ggcgattctg
     2821 aactacagcc gtggcctggc aaaacaggtc gcggagaaag gtattcgggt gaatattgtc
     2881 gcgccaggcc ctatctggac ggcactgcaa atttccggcg gacaaacgca ggataagatc
     2941 ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc aaccggcgga actggcccct
     3001 gtatatgttt atctggcaag tcaggagtcg agctacgtca ccgcagaagt gcacggtgtg
     3061 tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat
     3121 tgtgaattca ctcttacctg atgtggcgaa aacgccttat ccgcctacaa acgcatgaag
     3181 attcaacatg ttgcaataga ttttgtaggc ctgataagcg tagcgcatca ggcattgagc
     3241 acctaatgcc ggatgcggcg tgaacgcctt atccggccta caaacgcacg aagattcaac
     3301 atgttgcaat agattttgta ggcctgatga gcgtagcgca tcaggcaatt ctactttact
     3361 tcgctttggc ggtttcttcg ccgaccagac ctatcttcag gtaacccgcc tgatgcagcg
     3421 tatccattac cttcatcaac gtctcgtaat cgacggtttt atccgctcgg aagaagatgg
     3481 tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt aatcattgtt tcatcggtga
     3541 ccagatcgtt accgataaac atcgagttgt ctgccttcac cgacagataa accggttttt
     3601 ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt caccttcaca tctaccgtcg
     3661 ctaacggtgc cgccaccata aagataatca gcagaaccaa catcacgtcg ataaacggcg
     3721 tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca
     3781 tcggatatta tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca gcggcgctgg
     3841 cttccagatc caggtcacgg ctttgcagca acaatacctg cgctgcaaca tcacccagca
     3901 tcgctttaaa gccgccaatc tggcgtgcaa atacgttata gataacgacc gcaggaatcg
     3961 ctgccacgag gccgattgcc gttgctaaca gagcttctgc gatacccggc gcaacgactg
     4021 ccaggttagt ggtctgcgtt tgcgcgatac caataaagct gttcatgatg ccccagaccg
     4081 taccaaacag accaacgaac ggagaaatcg cgccgatggt tgccagatag ccgttaccgc
     4141 ggcccatttg acgacccact gcggcgaccc gacgctccag acggaagctg gtacgttctt
     4201 taataccttc gttatcgtcg ctgccttctg acagttccag ctcattctgc gcttcattga
     4261 acaaatgcag gcttaagctt ttgctaccaa aatcagcggc gatatcgttg gcctggttta
     4321 aggaacgcgc ttcagccagc agttgctgct cgcgctttag gcgacgcttc tgattgaaga
     4381 actctacgct cttactgaag aagattgccc aggtgactac ggaggccaaa ataagcccaa
     4441 tcatcacgca cttaacgaca atatcggcgt gctgatacat accccagacg gaaaggtctg
     4501 tctgcattaa attattaccc actgtgtatc tccaggacgc aagtcacaaa atctgcgcat
     4561 aataatatca aaacgacgtc gaattgatag tcattctcat tactatttgc atactgccgt
     4621 acctttgctt tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg
     4681 aaaattttct gctttatgcc aattcttcag gatgcgcccg cgaatattca tgctagttta
     4741 gacatccaga cgtataaaaa caggaatccc gacatggcgg acaaaaagct tgatactcaa
     4801 ctggtgaatg caggacgcag caaaaaatac actcttggcg cggtaaatag cgtgattcag
     4861 cgcgcttctt cgctggtatt tgagagtatg gaagccaaaa agcacgcgac gcgcaatcgc
     4921 gccaatggcg agttgttcta tggacggcgc ggaacgttaa cccatttttc cttacaacaa
     4981 gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat ttccctgcgg ggcggcagcg
     5041 gttgctaatt cgattcttgc ttttgtcgaa cagggcgatc atgtgttgat gaccaacact
     5101 gcctatgaac cgagtcagga tttctgtagc aaaatcctca gcaaactggg cgtaacgaca
     5161 tcgtggtttg atccgctgat tggtgccgat atcgttaagc atctgcagcc aaacactaaa
     5221 atcgtgtttc tggaatcgcc aggctccatc accatggaag tccacgacgt tccggcgatt
     5281 gttgccgccg tacgcagtgt ggtgccggat gccatcatta tgatcgacaa cacctgggca
     5341 gccggtgtgc tgtttaaggc actggatttt ggcatcgatg tttctattca agccgccacc
     5401 aaatatctgg ttgggcattc agatgcgatg attggcactg ccgtgtgcaa tgcccgttgc
     5461 tgggagcagc tacgggaaaa cgcctatctg atgggccaga tggtcgatgc cgataccgcc
     5521 tatataacca gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca tcatgaaagc
     5581 agtctgaaag tggctgaatg gctggcagaa catccgcaag tagcgcgagt taaccaccct
     5641 gctctgcctg gcagtaaagg acacgaattc tggaaacgag actttacagg tagcagcggg
     5701 ctgttttcct ttgtgcttaa gaaaaaactc agtaatgaag agctggcgaa ctatctggat
     5761 aacttcagtt tattcagcat ggcctactcg tggggcgggt atgaatcgtt gatcctggcg
     5821 aatcaaccag aacatatcgc cgccattcgc ccacaaggcg agatcgattt tagcgggacc
     5881 ttgattcgcc tgcatattgg tctggaagat gtcgacgatc tgattgccga tctggacgcc
     5941 ggttttgcgc gaattgtata acattgccac ttttggacaa ttttgcagac atttaattgt
     6001 gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc ggacgaatca ggagtacaat
     6061 aggcagataa aggcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat
     6121 catcgctgcg ctctggcaac acgactttgc cgcgctggcg gatcctcata ttgttagcgt
     6181 tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt
     6241 tttgccaggc gacagcttgt tgatactggc aggcgcattg attgcccagg gggttatgga
     6301 ttttctgcct acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta
     6361 tattcagggg cgctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc
     6421 tgctaaatat caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct
     6481 ggctggacgt tttcttgcat ttgtccgtac gctgctgcca accatggcgg gaatttccgg
     6541 tctgccaaac cgccgcttcc agtttttcaa ctggttaagt ggattgctgt gggtcagcgt
     6601 ggtaaccagt tttggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca
     6661 ggtaatgacg ttcctgatga tcctgccaat tgccttgtta accgctggct tgttaggcat
     6721 gctgtttgtg gtgattaaaa aaaaatactg taacgcctga cgattttccc cgttcccggc
     6781 tgctgtaccg ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc
     6841 ccggcgtcac accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc
     6901 cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg gatgatcatc atccgcgcct
     6961 tatgcagacg gtaattcttc aaatactgca acggcgaggt gctggtgaca gacttaaaat
     7021 tatggtggaa cgccgatacg ctcatgttgg cttctgccgc cagttgctcg acgctcaggt
     7081 tttcggtgta tttattctca atccgtttca gcacgcggct aatcagactg aagtgagtct
     7141 ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa
     7201 tttcgcggat gatctgtttg ccgagaatac gcgcatccag tggtcgctcc atcacgtcga
     7261 gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt ggcggagtta atcccgctgg
     7321 ctgccatcga cggctggaaa tgctcatctt cgccaatgtc catcaacagt tcctgtaact
     7381 gcaaaatatc gacattgaga cgcaaccctg ccagcggcac ctctgacgtg gcataggttt
     7441 cgcactcaaa cggcaacggc accgtcagca gcaggtattc attggcatca taacgaaaca
     7501 cgcgttcatt gatataaccg attttatgcc cggaaaagag aattatgatg ccaggctcgt
     7561 acatcaccgg tgtacgtgcg aaaggcgtct caccatacaa caaacgcaca tcgggcaaca
     7621 gttctgacaa actattttct ttatttttca gtttattaac tttatccgcc agcaagcggc
     7681 aaatctcttc acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg
     7741 cgcaattttg tagcatttct ccagcactct ggagaaatag gcaagacatt ggcagaaatg
     7801 agcattgaga gccagggcgc tggcgatcac aatgaaaaac atcaggcaga tcgttctctg
     7861 ccctcatatt ggcccagcaa agggagcaag taatgaacaa ctttaatctg cacaccccaa
     7921 cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg
     7981 ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag
     8041 ttctggatgc cctgaaaggc atggacgtgc tggaatttgg cggtattgag ccaaacccgg
     8101 cttatgaaac gctgatgaac gccgtgaaac tggttcgcga acagaaagtg actttcctgc
     8161 tggcggttgg cggcggttct gtactggacg gcaccaaatt tatcgccgca gcggataact
     8221 atccggaaaa tatcgatccg tggcacattc tgcaaacggg cggtaaagag attaaaagcg
     8281 ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc aacgcaggcg
     8341 cggtgatctc ccgtaaaacc actggcgaca agcaggcgtt ccattctgcc catgttcagc
     8401 cggtatttgc cgtgctcgat ccggtttata cctacaccct gccgccgcgt caggtggcta
     8461 acggcgtagt ggacgccttt gtacacaccg tggaacagta tgttaccaaa ccggttgatg
     8521 ccaaaattca ggaccgtttc gcagaaggca ttttgctgac gctgatcgaa gatggtccga
     8581 aagccctgaa agagccagaa aactacgatg tgcgcgccaa cgtcatgtgg gcggcgacgc
     8641 aggcgctgaa cggtttgatt ggcgctggcg taccgcagga ctgggcaacg catatgctgg
     8701 gccacgaact gactgcgatg cacggtctgg atcacgcgca aacactggct atcgtcctgc
     8761 ctgcactgtg gaatgaaaaa cgcgatacca agcgcgctaa gctgctgcaa tatgctgaac
     8821 gcgtctggaa catcactgaa ggttccgacg atgagcgtat tgacgccgcg attgccgcaa
     8881 cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct ctccgactac ggtctggacg
     8941 gcagctccat cccggctttg ctgaaaaaac tggaagagca cggcatgacc caactgggcg
     9001 aaaatcatga cattacgttg gatgtcagcc gccgtatata cgaagccgcc cgctaagctt
     9061 tttacgcctc aaactttcgt tttcgggcat ttcgtccaga cttaagttca caacacctca
     9121 ccggagcctg ctccggtgag ttcatataaa ggaggaacgt atggctaatc caaccgttat
     9181 taagctacag gatggcaatg tcatgcccca gctgggactg ggcgtctggc aagcaagtaa
     9241 tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct cgattgatac
     9301 cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa
     9361 cagagaagaa ctgttcatca ccactaagct gtggaacgac gaccacaagc gcccccgcga
     9421 agccctgctc gacagcctga aaaaactcca gcttgattat atcgacctct acttaatgca
     9481 ctggcccgtt cccgctatcg accattatgt cgaagcatgg aaaggcatga tcgaattgca
     9541 aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc acctgcaacg
     9601 cctgattgat gaaactggcg tgacgcctgt gataaaccag atcgaacttc atccgctgat
     9661 gcaacaacgc cagctacacg cctggaacgc gacacacaaa atccagaccg aatcctggag
     9721 cccattagcg caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg atctggcaga
     9781 taaatacggc aaaaccccgg cgcagattgt tatccgctgg catctggata gcggcctggt
     9841 ggtgatcccg aaatcggtca caccttcacg tattgccgaa aactttgatg tctgggattt
     9901 ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc gatcagggca agcgtctcgg
     9961 tcccgatcct gaccagttcg gcggctaaca tgcaaattct cccggtggcg gtaatgttcc
    10021 gctaccggac ttttcagaaa tcatttattc ccctcgcgtc ccgcccgttg ttactcttcc
    10081 ttgttcagga atgccaaata taaggacatc atcatgcaga gccggaagct cttaaaagaa
    10141 caactcatct atatccggga taaacgcaac ggagaggtga aaaacagatg aaaataatac
    10201 ttctgttttt agcagccctg gcaagtttta ccgtacacgc gcagcctcct tcacagaccg
    10261 tagaacaaac agtccggcag atttatcaga actataaatc agatgccagt acgccttatt
    10321 tcggtgaaac cggagagcgg gcgataactt cagcgcgtat tcaacaggcg cttaccctga
    10381 acgacaatct tacgctgccg ggcaatattg gctggctgga ttatgatccg gtttgtgatt
    10441 gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat aacccaaact gacgccgatc
    10501 atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag accacgcaga
    10561 cactgaaaat ggtggcggaa aatggtcgtt gggtcattga cgatattgtc agcaatcatg
    10621 gcagtgtctt acaagcagtt aatagcgaga atgaaaaaac gctggccgct ttagcttcgt
    10681 tgcaaaaaga acagccggaa gcctttgttg ccgaactctt tgaacatatt gctgattata
    10741 gctggccgtg gacgtgggtg gtttccgact cttaccgcca ggcggttaat gccttctata
    10801 aaaccacctt caagacggcc aataatcccg atgaagatat gcaaatagaa cggcaattta
    10861 tttacgacaa tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc
    10921 gagtcctgga gaaaaccgcc gattccgccc gcattcatgt tcgttttacg ctgaccaatg
    10981 gcaacaacga agagcaagaa ctggttttac agcggcgcga aggcaagtgg gaaatcgctg
    11041 attttatccg cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa actgccgcca
    11101 gattaaagca atgagctgaa ttaaataaca attagccgga acaataaata aaagggaaca
    11161 ctatatgaaa acgattttca ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg
    11221 ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat gtgcaaacat taaatgccaa
    11281 aatcgcccgg cttgagcagg atatgaaagc actacgccca caaatctatg ctgccaaatc
    11341 cgaagctaac agagccaata cgcgtcttga tgcacaggac tattttgatt gcctgcgctg
    11401 cttgcgtatg tacgcagaat gataaaaaaa tccccggcag catgttagtt gccgggttat
    11461 aggctgtttg ctaaaaaaaa gccaaacata aggacatgat tatgcgacgc tggcacacct
    11521 gtttttttat tctgctgttc tgtggcttca gcggaaacgt gttgtgcaaa gaatcgatta
    11581 atgaagcgat agaacgtttg taccaaccat tgaccagtaa tgacgatata ccaataaccg
    11641 atgaattcag tctcgatttg cgaagtaata tgagcgctac tcctggcaaa atgacaccct
    11701 ggctgaataa taatcttttg tgtcgctgtg tcgatgacgc tttcgtttct gcagaaataa
    11761 tccaaagcac acacctaaat ggcggccgaa cgcatgtact gttgcagctg gttatcaaac
    11821 aaaatcgcac aga
//
LOCUS       DAJVOY010000123        11816 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVOY010000123 DAJVOY010000000
VERSION     DAJVOY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.123"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(37..273)
                     /locus_tag="OQL90_002929"
     CDS             complement(37..273)
                     /locus_tag="OQL90_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1369635.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="OQL90_002930"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="OQL90_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1369636.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1916)
                     /gene="bglJ"
                     /locus_tag="OQL90_002931"
                     /pseudo
     CDS             complement(1240..1916)
                     /gene="bglJ"
                     /locus_tag="OQL90_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(1874..2599)
                     /locus_tag="OQL90_002932"
     CDS             complement(1874..2599)
                     /locus_tag="OQL90_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1369637.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3218..3988
                     /locus_tag="OQL90_002933"
     CDS             3218..3988
                     /locus_tag="OQL90_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1369638.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     HHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3979..4452
                     /locus_tag="OQL90_002934"
     CDS             3979..4452
                     /locus_tag="OQL90_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1369639.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="OQL90_002935"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="OQL90_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1369640.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="OQL90_002936"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="OQL90_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1369641.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /locus_tag="OQL90_002937"
     CDS             5888..6382
                     /locus_tag="OQL90_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1369642.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="OQL90_002938"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="OQL90_002938"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1369643.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="OQL90_002939"
     CDS             complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="OQL90_002939"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCS1369644.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10227..11141
                     /locus_tag="OQL90_002940"
     CDS             10227..11141
                     /locus_tag="OQL90_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1369645.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11356..>11816
                     /locus_tag="OQL90_002941"
     CDS             11356..>11816
                     /locus_tag="OQL90_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1369646.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKV"
BASE COUNT         3070 a         3013 c         2762 g         2971 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aactaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaatggtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca cccctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaatg
     1741 accgccgcaa atgagatccg cgacatggca tcctgaaatg actcctgact ggtataggta
     1801 tggagcttat aagggttacc cgcaaacttt ctgaataaac tctcaatacc gatactgctc
     1861 atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg ttccattctg
     1921 cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg ctatttatcc
     1981 atgcatacat ttcagcgttg ctgtgaattg ccagtcgacg catcgcactg tttttttggg
     2041 cgctgatagt tttattactt ttcttaagca acgaaactat ctggttgatg ccccatcctt
     2101 tacctaatag ccgcagtacc ttgcgctctg aaagagtgag aatgacggag cgatagctaa
     2161 aatcatgagt ttcattcatt gccggagagg tcagcatggc gctgatacgc tctgcgtgcg
     2221 tattgccgga acgtacggtc ttgaccagat tctcaatggg ttcaacatca gacaataacg
     2281 tcgccgtagg gcacatgagc aattctactg cctgggaata ccaggagcgt gacaccatga
     2341 aaacccagtg aatttcccga tattgtgaga ttaaagaata ataatgttca caaacagaac
     2401 gtggatcttc actttcaccg gctaaatcag caatgactaa tccggaacga cgtaattgta
     2461 ataaggtaag gtcctccgca gaggcgctgg atattatttc atattcaggg aagtgagtcc
     2521 tcatgacctc ttttaaccct gcttgcataa caggtatttt actgataaca attccatttt
     2581 tgcagcatcc tggcaacata tttcctccac atccactatc taaaaatcat gatggtttat
     2641 tcaggatatt cattccttaa atgtttccag caaattaata gattaagcga ataaattatc
     2701 cttagcattc actttcatat cagcatactc ctgctgcaca acaactccac tgcatgtgtc
     2761 agaaggttag catcaaatga taacctagtg aaatagcctg gagacattta aaatatctac
     2821 attaagttgt taggaatact taatgaaaaa cagaaatcac attcatttaa tttataatta
     2881 aatgatatcc cttcccgcag aatgaaaagt cacacaacca atatttattc aatgaaatct
     2941 gatggtttta acttgttaac attaaaaaac gtgcccggtt atctattatc ctgattcgta
     3001 aaggccaaac aataaattca ttatagtgat aatattcatg tgatagtttg tttgcggcga
     3061 gagataattc gctttttatc accgagcttg tcccgataac caggattaat cagcgcaatt
     3121 ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata caaatggatt ctcattatct
     3181 gaacaatacg cagcacgtct atgacaaagg gcgagttatg caaactgagc aacagcgagc
     3241 cgtaacacgg ctttgtatcc agtgtggatt atttcttttg caacatggtg cggaaagcgc
     3301 gttggttgat gagctttcct cacgactggg tcgggcgttg gggatggaca gcgtcgaaag
     3361 ttctatctct tcgaacgcca tagtgctgac aaccattaaa gatgggcaat gcctgacatc
     3421 gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg gtgactgaag tccagcacat
     3481 tgtgattctg gcggagcacc atctgctgga ttacaaaggc gtagagaaac gatttagcca
     3541 aattcagcca ttacgttacc caagatggct ggtagcctta atggttggcc tttcttgcgc
     3601 ctgtttctgc aaactcaata aaggtggctg ggatggtgcc atcatcacct tctttgccag
     3661 tacggccgcg atgtatatcc gccagctgct ggcacaacat catcttcatc cgcagatcaa
     3721 cttttgcctc accgctttcg ccgccaccac catttccgga ttgcttttgc aacttcccac
     3781 tttcagcaat acacccacta tcgcgatggc cgccagcgtt ctgctgctgg tgccgggatt
     3841 tccgttgatt aatgccgtcg ccgatatgtt taaaggccac atcaataccg gactggcacg
     3901 ctgggcgatc gccagtctgc tgacactggc tacctgcgtc ggcgtggtga tggcactgac
     3961 gatttggggg ctacgcggat gggtgtgatc gagtttctgt ttgcattggc gcaggatatg
     4021 atcctcgccg ccattccagc ggtcggcttt gcgatggtgt tcaacgttcc cgttcgggca
     4081 ttacgctggt gtgcgctgct tggcgcgatt ggtcatggtt cacgaatgat cttaatgacc
     4141 agagggctga atattgagtg gtcaaccttt atggcctcca tgctggtcgg caccattggt
     4201 attcaatggt cgcgctggta tctggcgcat ccgaaagtgt ttaccgtggc ggctgttatc
     4261 cctatgttcc cgggcatatc ggcttatacc gcaatgattt cggcggtaaa aatcagccag
     4321 ttaggttaca gcgaaccgtt gatgattacc ctgttaacca actttcttac agcttcatcg
     4381 attgttggtg cgttatccat cggtctttcc attcctggat tatggttgta ccgcaagcgc
     4441 cctcgcgtat aaaattgcct cgcttaaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4801 ccgttgctgt acccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgatt
     4861 ttatccgcct ggcggcgcta tggggtgtgg cactaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagc gaacctgaca gtcaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgcattc aaaacgggtg aagaactact ggcctggcag aaagaacaag gagcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgg ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtacgtcgaa gagtttgacg gcaacatcgc
     5401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagtgaa gaacaactgc ttaacgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6001 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6121 tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa acatcaagaa
     6181 ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6301 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgcttc tggacggttt tccccctgtt acacgggaag
     6481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtt cttctcaaaa agcttaaacg
     6601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6721 cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccatcgtcg ccatcatccg caccattttg
     6961 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg
     7321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     7501 aaagcgtggt ggccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt tgcagccagg
     7741 agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa gataccgtca
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc
     7861 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg
     7921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8221 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctat aagatggcac aactctgggc tccggaactg gcactctcca
     8341 ccgactggtg tgtctcgcaa gggcagcttg gcggtcagca aattgttcaa catgttgaca
     8401 aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg gcgcgttata
     8461 aaggcaatgt cgatacgctg aaaattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg atttcccgtc
     8581 cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     8641 agcatccgct gccaaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgtg ctgggcaatg
     8761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccacgtaagc
     8881 tcgggatcgg catggtggaa attaaagtgg tggaacgtga aggataattc ctgactgatg
     8941 tgtaggtcgc ataaggtgcg acctacacac cttctgcggc ctacaaaaca tcattgagaa
     9001 acagaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg
     9061 ccttatcaga aagtaattac acccttaatc aactcacgat tgttaatcac atcgcgctcg
     9121 taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc agcagtgatt
     9181 tttccttccg ccatcagtcg accgacttta gcaaaatctt ccggcgtggc gttgcggcta
     9241 cccatcatcg tcgtttcttt tttatggaat tccggatcgg agaactgcaa ctcaccttta
     9301 aacaggccga caaataccac cgtgccgccg tgacgaatca aattcacggt gttattcatc
     9361 gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc
     9421 cgtagctgcg cgtcaaaatc ttcggctgac gggtccagta aaggtaattc cagacgcgtt
     9481 gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc gccatcggct
     9541 ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg
     9601 ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat
     9661 gctgccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa
     9721 tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg
     9781 gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa cataagggat cacagcaact
     9841 tgcagaccat ttttaagatc agcaatattt ttacccagcc taacaatctc cccacatatt
     9901 tcatggccta aaacacgtgg ataactaaaa aatggttgat ttccacccca ggcatgaata
     9961 tcggtaccgc aaatcccgac agacttaatt tttattaatg cttcattgtc acccggaata
    10021 ggtatctcgc gttgtttcca gactaattct ttcggctgct ggcaaattaa aacattcatc
    10081 gtagacatga ttcggctctc cattttttgt tgccttagat atcaatgaaa atgaaaaatg
    10141 gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt taaatcgggt
    10201 tttaatggac tcacaaggag gaaaatatga gtcgttcaca aaatttacgc cacaatgtga
    10261 ttaaccaggt cattgatgat atggcgcggg gtcatatccc ttccccactt ccatcgcaaa
    10321 gtgcgctggc ggaaatgtac aacatcagcc gcaccacagt gcgtcatatt ctcagccatt
    10381 tacgcgaatg cggcgtcttg acgcaggttg gaaacgacta tgttattgcg cgtaaacctg
    10441 accacgatga cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac aaagtgtttg
    10501 agcaggcttt tttcacgctg ataaaccagc gccagttacg cccaggggaa actttttctg
    10561 aactgcaact ggctcgagcc gcgggtgtca gcccggtggt tgtaagagaa tatcttttaa
    10621 aattcggccg ttataatctt attcaaagcg aaaagcgcgg ccagtggagc atgaagcaat
    10681 tcgatcaatc ctacgccgag cagctgtttg agctacgcga aatgctcgaa acacattcac
    10741 ttcagcactt tctcaacctg cctgatcatg atccccgctg gctgcaggcg aaaaccatgc
    10801 tcgaacgtca tcggctactg cgtgacaaca tcggcaatag ctttcgcatg ttctcgcaac
    10861 ttgacagaga ttttcactcc ttactgcttt cagctgccga caatatcttt tttgatcaat
    10921 cgcttgagat catctcagtc atctttcatt ttcactatca atgggacgaa agcgatctta
    10981 aacagcgcaa tatcattgcc gtcgatgagc atatgaccat tctcagcgct ctgatttgcc
    11041 gcagtgacct cgatgccacc cttgcgctcc gtaaccattt gaattcagct aaacaatcaa
    11101 tgattcgatc cattaatgag aacacgcgtt atgctcatta agtacggatt aaaaacagca
    11161 aactattagc gggtgcacgg ttttatcaaa agataaatac cgggaaattc ccgcttacct
    11221 atgctcacaa tcagtcgact aaaaatgttt ttgtgagcgg tagtaaagtc ctaaaacttt
    11281 aacctgccgt taatgtcaac cttgcgtaat gcacaattac gtaaacaatt aaaattataa
    11341 atacaggagt ccggcgtgga aaaagaaaat atcaccatcg atccgcgttc ttcatttact
    11401 ccatcttcgt cggcagatat tcccgtgcca ccagatggat tagttcaacg cagtacccga
    11461 attaaacgca ttcaaaccac cgccatgttg ttattatttt ttgcggcggt aatcaattat
    11521 ctcgaccgca gttcgctgtc ggtagcaaat ttaacgattc gtgaagaatt gggattaagt
    11581 gccactgaaa tcggcgcttt gctctccgtg ttttcactcg cttacgggat tgcgcaactt
    11641 ccttgcggcc cactattgga tcgtaaaggc ccacgcctga tgctgggact ggggatgttc
    11701 ttctggtcac tgttccaggc aatgtctggc atggtgcaca actttacgca gttcgtgttg
    11761 gtgcgtatcg gtatggggat tggtgaagcg ccgatgaacc catgcggtgt aaaagt
//
LOCUS       DAJVOY010000124        11736 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVOY010000124 DAJVOY010000000
VERSION     DAJVOY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.124"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            428..1084
                     /gene="pphA"
                     /locus_tag="OQL90_002942"
     CDS             428..1084
                     /gene="pphA"
                     /locus_tag="OQL90_002942"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCS1369647.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1085..1276)
                     /locus_tag="OQL90_002943"
     CDS             complement(1085..1276)
                     /locus_tag="OQL90_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCS1369648.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1381..1617)
                     /locus_tag="OQL90_002944"
     CDS             complement(1381..1617)
                     /locus_tag="OQL90_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCS1369649.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1735..3174)
                     /gene="rsmF"
                     /locus_tag="OQL90_002945"
     CDS             complement(1735..3174)
                     /gene="rsmF"
                     /locus_tag="OQL90_002945"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS1369650.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3254..5887)
                     /gene="yebT"
                     /locus_tag="OQL90_002946"
     CDS             complement(3254..5887)
                     /gene="yebT"
                     /locus_tag="OQL90_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCS1369651.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5856..7139)
                     /gene="yebS"
                     /locus_tag="OQL90_002947"
     CDS             complement(5856..7139)
                     /gene="yebS"
                     /locus_tag="OQL90_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS1369652.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7269..7766
                     /gene="msrC"
                     /locus_tag="OQL90_002948"
     CDS             7269..7766
                     /gene="msrC"
                     /locus_tag="OQL90_002948"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1369653.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7863..8561
                     /gene="proQ"
                     /locus_tag="OQL90_002949"
     CDS             7863..8561
                     /gene="proQ"
                     /locus_tag="OQL90_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1369654.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8581..10629
                     /gene="prc"
                     /locus_tag="OQL90_002950"
     CDS             8581..10629
                     /gene="prc"
                     /locus_tag="OQL90_002950"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1369655.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10821..11702
                     /gene="htpX"
                     /locus_tag="OQL90_002951"
     CDS             10821..11702
                     /gene="htpX"
                     /locus_tag="OQL90_002951"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1369656.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         3014 a         2889 c         3030 g         2803 t
ORIGIN      
        1 ggctgatgga tgttcgatag cgcgagtttg aatgaaaatc agccggagat gattttacat
       61 aattgctacg gaattattca atacaggaat tgcttgcgtc tgcatggatt gacctgaaat
      121 tttcccgaaa atttctctaa aaaactcgaa aaaaatggta actggttgaa tgtattaata
      181 tgcaatggta cgtgtcaggg attaaaagat gaacgtaaat ttattcaacg cattaatttt
      241 aaagggtttt attgtttgtt gacgaaaaca ggaatcgtgt tcggtctctt tttatctgtt
      301 aaaagccaga agcatttcct tcgctgactt tatagtcaac cataacacac actctactgt
      361 ctgagtccag cgttttttaa cattcttgtt aagattatgt gatctttagc acgggaggaa
      421 aatattgatg aaacagcctg cgcccgttta tcagagaatt gcgggtcatc aatggcgaca
      481 tatctggctt tctggcgata tacacggttg tcttgagcag ttgcgccgca aattatggca
      541 ttgtcgtttt gatccgtggc gagatttact tatctcagtg ggagacgtta tcgatcgtgg
      601 gccgcaaagt ttacgttgtc tgcagttact ggaacaacat tgggtttgtg cggtaagagg
      661 caatcatgaa cagatggcga tggatgcgct ggcatcccag cagatgtctt tgtggttgat
      721 gaatggcggc gactggttta ttgcgctggc agataatcaa cagaaacaag cgaaaacggc
      781 gctggaaaaa tgtcagcatt tgccctttat tcttgaagta cacagtcgca ccggcaagca
      841 tgttattgct catgcggatt atccagatga tgtttatgaa tggcaaaagg acgttgattt
      901 gcatcaggtc ttgtggagcc gctcgcgatt aggtgaacgc caaaaagggc agggaattac
      961 aggtgctgat catttctggt ttggtcatac accgttgcga catcgcgtgg atattggcaa
     1021 cctgcattat attgataccg gtgctgtctt tgggggcgaa ctgactcttg tgcaattgca
     1081 atgattaaaa atcaccgtac tcctgtgcag gtcgccagaa accatctata aaatcctcaa
     1141 tcggaaaaca accgccctgg cggatccgtt gatcgctcat agaataaaga cactgctgtt
     1201 ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag caaacaaaaa
     1261 gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt ttaggagaga
     1321 ttttacaacg ggggaataac caggacaaat aacccgccat gccgacgggt tctttttgga
     1381 tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac ggtaaaggac
     1441 agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg catcgagttg
     1501 catacataaa tcggggtcag atttacaagg aatggttaac gttcgatcac ccggcgattc
     1561 accgtgtaat tcgccgtcgt cgatttcaaa agcgcctatg cgcacactgg ttttcatctt
     1621 cgtgctctcc atttagctgc cggttgtggt acgaccagtc cgatcgcacc atttttcagc
     1681 ctagttgagg cagccgaatg tgccagtcaa aaagtgcgct ttttttgtgc gctgtcaggc
     1741 gttaccggta aaaagtttcc catcgcgcac cagttcacgc ggatagctgt ttttcaatcg
     1801 cgaaccaatc cgtttggcta aaccaatcgg ttgatgctgg aaagtaacca acacgtcatc
     1861 cgccactggc gcggcttgcg ggtaaacatc gcgcccgcga taccactcct ccgcttcctg
     1921 cggtgtcagt tcaaaagcgt tcacattgtc gggggtggca agggcaataa ctgcttcatg
     1981 ctgccagcga taacctttgt tgtgcgtttc ggcaagttta atccccaagc gagaaaatcg
     2041 gactttaccg atcagggctt caatgcccac cgggaacaac cacagttctt tgtcacgctg
     2101 ccagaggcgc aggttttcat cccagtttaa gccaacacct gcagccgcct ggcgaatttg
     2161 tccagcttcg cgatctttca ccgggctgaa cggaaaatta ccgactttgt atttgggggc
     2221 gggtaaggcg ggaatcgcct gagttttacg cagacgagca acgaagaagc cttcgcagtc
     2281 gtaaatttgt gggaaaacat gcaaaaagcc ttcttcggtc agcgctttgt ttgcaccagg
     2341 gaagagatcg ccaagtggta aaaactctac tgcgtcgggg taagtctctt tcagccacag
     2401 gcaaacggct tcgttttctt cctggtttaa ggtacaggtc gagtaaacca gcgtaccacc
     2461 aggacgtaat gcatgaaagg cgctgtcgat aagctcccgt tgtgtagctg cgatttcctg
     2521 attgctttct ggtgaccagt tttttagcgc atcgggatct ttacgcacca cgccttcgcc
     2581 ggagcaggga gcgtccagca aaatggcatc aaacatttct ggcactgccg caccaaacac
     2641 gcggccatca aaatgtgtga gcgcaacatt actgatgcca cagcggctga tattggcatg
     2701 taacactttt acccgactgg cggaaaactc attggcaagg attgcccctt cgttattcat
     2761 ccgcgcggca atttgcgtcg tttttgagcc tggcgcagca gcgacatcca tcacccgctg
     2821 tggtgcatta ccgtcagcaa acaaggcggc gacgggcaac attgaactgg cttcctgaat
     2881 ataaaacagg ccacttaaat gctcggcggt actacccaat ggcaatgcat cttcattgtc
     2941 gcgttcaatc cagaaacctt cttcacacca cggaattggc gtaagcgtcc agccataagg
     3001 agcggttaat tgcaggaaat cagcaacgga gattttcagc gtattaacgc gaatgctgcg
     3061 gcgcaacggg cgctgacagg cggcaagaaa atcatcaaat gagagcgtcg aaggcatagc
     3121 ttcgcgcatt tgtgtcagaa acgcgtccgg gaaataaacg gtgtgttggg ccacgagcat
     3181 gtaccactgg caaaaaaaca ggcgcgcagt ttagcataaa cgctccggcg caggcacgcc
     3241 ggagcagtgg gcattatttg ggaagcgcag ttccccattc acgccactct ttcggttcac
     3301 tttcctgcaa caggaagtgt ttgccttcct gggctttcgg tgccagtggc gtacccggag
     3361 gcgtggcgaa ggcgatgccg ccacggataa actggttaaa ggtgccggtt ttcactacgc
     3421 cgcccgtcag accaaagtcc agactgtaac ccgatgccaa ccagaagacg gaattgttac
     3481 gcaccaggtg ttgatagcgt ttactgatgc gcatcgcaat catcacgcga tctgacaatg
     3541 tccccagcgt cattccggta accgtaccta cttccagacc acggaacagc acaggcgtac
     3601 caatacctaa cgaaccggct tccggtgctt cgacaataat gcttaagcca tccaggtaac
     3661 gcgaatcagt aatagtagct tcttgcaact caaagtcacg gcgaggatta ccccgacctg
     3721 gttcgacatt gatatacggc tggaggatag tatctagatg ctcaacgccc gtggctgaaa
     3781 tttgcggtgt gaccaccgag aagcgcgtac cgccgcgggc gaaggtctgg acatactccg
     3841 gatagagcac cgcctttgcc tgtacttcat tgcgcgcggt aatcagatcc agcgtctgga
     3901 tttgcccgat atcaataccg agatagcgaa ttggcatccc gaccgccagt tttccggcat
     3961 cgaaagcgtg aagcgtaatc tgcccactaa ccgcacgggc cgctgtttcg gaagcataca
     4021 gaattcgttt gtcgccttta cgctgactgg cgctggcacc gctgaggtta tcgaagctaa
     4081 tggctccctt taatgctctg gagagcgggg atgcctgtac ggtcaggcca ctaccattca
     4141 gctgaacttt cgccccgcct tctgcccaga acacgctatt gctggtcaga aggttgcgat
     4201 actccggctt aatatgcaga tcgatatcaa acgcgttagc tcgcggacgc acggtaatca
     4261 cttcaccaac ttcaaattta cggtacagca ctaccgatcc tgcctgcaca tccggcagcg
     4321 tctctgcact caaactcacg gttgtggtgg gtaaatcgct aaggctgttc tccagcgctt
     4381 tttccagatt ggcatacagt ggatagctgg ctttcatctc acctttatcg cctggcagaa
     4441 tacgtatccc gccgttaatc cattctgagg cgctggcacc aagaaactca acgccatcca
     4501 gccccacctt cacgtcgaca cggctgttga cgacaaattt gctatcgcct tttaccagtt
     4561 cgcgatgctg aggctcgatg gcgacggtaa aggtaacgcc tttgctggtg agtttacgat
     4621 caataacctg gcctacctgc acgccgtgaa gaatgagcgg ctgacccgta tcaataccgt
     4681 aactttccgg cgcggtcagg gtcagcgtca gaacatcagg ttcatgcagc agtgcttttt
     4741 cgcctggcac aacaacgaac tctttgcgtg gctcgccatc gccgggtacc aactcgaagg
     4801 ttttgccagt cagcagggcg ctgagattgg catcgctaag ggataatttc ggattacgta
     4861 attcgatgcg ggtattttca cgaagcaggg taacgacgct gggatcaact gtcatttccc
     4921 cagtgacttt accaccagga tttaaatcca gtttagttag ctgtccgact tccagtccct
     4981 gatacattaa tggcgtcgag tcggcggtta atccggcccc actcggcagt tccagtttga
     5041 ttattacgcc acgctggcta tgggcgaggt cttcatacag accaaaggta tcttccgcct
     5101 cggcaggttt cgactcttct ggtgaatcga aggcaatcgc accgttaacc agtgccgcca
     5161 ggctttccag tttcaccttc gcgccactga tactgacatt ggcatcaacg ccggaaacgt
     5221 tccagaaacg gctacctttt ttcaccagat cggtaaaacg ccgctcgatc aggacatcaa
     5281 tcaccacgcc ttgtttgttg ggattgatgg catagtcgta gacttttccc accgggatct
     5341 tgcggaaata gaccaatgaa ccgctgttca gcgaaccgag atcgggggct tgcaggtgga
     5401 tcatcagatc gccattgttc agccgatatt tcggttgggt atcgagtgcg acaaagtgat
     5461 cctgctcttt acctgtaccc ggcatcatgc cgatatagtt cccaccgacg agggcgtcca
     5521 gcccggagac acctgccaac gatgcttttg gcgtcaccag ccagaattga gtctcttcgc
     5581 gcagcgcatc tttcatatcg gacttgatgc tgaccttgac ttcaatctta cgaagatcgt
     5641 cgctgaggct gatatcctgc actgttccga cttcaacgcc ctgataacga acaggcgtac
     5701 ggcccgggac aataccatcc gccgacataa agtcgatggt gacggtatta cttcggtcct
     5761 gataactgtc ccaaatcagc caaccggcaa tcattagcgc gatgaaaggc agcagccaga
     5821 aaggtgagat acggcgttta tttttaatct gcgcttcagt cgtcgaagcg ggcgtttcct
     5881 gactcatgtg catcccaaag taagcggctg tccagccatt ccacagcaag aatagtcaaa
     5941 attacggctg cgccaaaata aaacgcagcc ggtcccatag taaaagcgag gatctgatcg
     6001 cgattaatca gcgacatggt taaagatatg acaaacaggt cgagcatcga ccagcgtcca
     6061 atccaggtca ccatccgcag taacagaatg cgtgtgcgta aaccttgctg gcatttaaaa
     6121 tgaatgctca acagtaaagt gaacatgacg atcactttag taaacggtac cagaatactg
     6181 gcgataaaca cgattccggc gaccgcaatg ttgctacttg ccagcgacat aatcccggaa
     6241 agaatcgtat cttcctgccg gccaccattc agataaatga tagaaatagg caataggttg
     6301 gctggtaaca acaaaacgat tgacgctaac agcgccgccc agcatttttg caaactatga
     6361 cgacgacgca ggcgtagcgg gatatggcaa cgcgggcagc gaccacgctg atcaggatag
     6421 ccggtaaaat ggcacccaag acagacacga agtttctcgt ccctacgcgt agcggggcgc
     6481 tgcggataat atcgctccca cagctcctca acattaagat gtgacaacgt caccgtcgtt
     6541 aaaatcacca acgccacaaa agagaacaag ccgacacccg cctggatatg ggcataatcc
     6601 tgtaccttta tagaagcaac gccaatgccg accaggtaga tatccagcat tacccactct
     6661 ttaagacgct caagcatcag cagtaccgga cgtaaattca ttcccagtcg gttaccaaac
     6721 cataaatatg ctatggagga caccagaatg aggggggcac caataacgca gaaaaagacc
     6781 atcgcccccg ttatcgtatc gccctgtttg gtcatttgcc agatgccttg catgacgttt
     6841 gcgtcgatac gaatacctaa cagccagata tgcaacagcg gttcgcccca ggcaaacggc
     6901 atcagcaaca gcatggtgaa agccattgcc gccaggcgcg ttagcgacca gtcgcgcccg
     6961 tcgcgaattt ttgcctgaca gcgcggacaa taggcactct gatgagaatt tatctcgggc
     7021 aggctaaaca gcatgtcaca ttgcgggcaa cgttggtaat caccacgcgg cagttcctcg
     7081 ccgattgacc tcactgttat cttttttgtc ggcgtaattt gtggtgtgtt aagagccatg
     7141 acgtgccaaa tgtaataaga attaacatta atcttaaccc atgatgggaa tgatcttgag
     7201 cattaacgca tttaatgctt attttaatag gctggcagtt ccaggtaaga caaacaagtg
     7261 attatataat gaacaaaaca gaattttacg cggatttaaa tcgcgacttt aacgcgctga
     7321 tggcgggaga aaccagtttt ctggcaacgc ttgcgaacac cagtgcgttg ttatacgagc
     7381 gtctcactga cgtaaactgg gcaggttttt atttgcttga ggacgataca ctggtactcg
     7441 gaccatttca gggcaaaatt gcctgtgtcc ggatacccgt cgggcgcggg gtgtgcggca
     7501 ctgcggttgc ccgcaatcaa gtgcagcgta tcgaggatgt tcacgcgttt gacgggcata
     7561 ttgcctgtga tgcggcgagt aattctgaaa ttgttctgcc gctggtggtg aaaaatcaga
     7621 ttattggtgt tcttgacatc gatagtaccg tcttcggtcg ctttacagac gaggacgagc
     7681 aaggcttacg tcagcttgtg gcacagcttg aaaaagtgct tgcaacgacg gattacaaaa
     7741 aattctttgc gagcgtcgca ggataatcaa cggataacgt agcatttact ggtagcgtca
     7801 ttataatgac gcctgttcat gcctgcgctt gttggctacg tccgttgtaa tcaggaaatt
     7861 tcatggaaaa tcaacctaag ttgaatagca gtaaagaagt aatcgcgttt ctggccgaac
     7921 gttttcccca ctgtttcagt gcggaaggtg aagcgcgtcc gctgaaaatc ggtatttttc
     7981 aggatttggt cgatcgtgtt gccggggaaa tgaacctgag caaaacgcaa ttacgatccg
     8041 ctttacgtct ctacacttcg agctggcgtt atctttacgg tgttaaaccc ggcgcaacgc
     8101 gtgtcgatct tgacggcaac ccatgcggtg agctggacga gcaacatgta gagcatgctc
     8161 gcaagcagct tgaagaagcg aaagcgcgtg ttcaggcaca gcgtgctgaa cagcaagcga
     8221 aaaaacgcga agctgccgca gctgctggtg agaaagaaga cgcaccgcgc cgcgaacgca
     8281 agccacgtcc gaccacgcca cgccgcaaag aaggcgctga acgtaaacct cgtgcgcaaa
     8341 agccggtaga gaaagcgcca aaaacagtaa aagcacctcg cgaagaacag cacaccccgg
     8401 tttctgacat ttcagctctg actgtcggac aagctctgaa ggtgaaagcg ggtcaaaacg
     8461 cgatggatgc caccgtatta gaaatcacca aagacggcgt ccgcgtccag ctgaattcgg
     8521 gtatgtcttt gattgtgcgc gcagaacacc tggtgttctg aaacggaggc cgggccaggc
     8581 atgaacatgt tttttaggct taccgcgtta gctggcctgc ttgcaatagc aggccagacc
     8641 ttcgctgtag aagatatcac gcgtgctgat caaattccgg tattaaagga agagacgcag
     8701 catgcgacgg taagtgagcg cgtaacgtcg cgcttcaccc gttctcatta tcgccagttc
     8761 gacctcgatc aggcattttc ggccaaaatc tttgaccgct acctgaatct gctcgattac
     8821 agccacaacg tgctgctggc aagcgatgtt gaacagttcg cgaaaaagaa aaccgagtta
     8881 ggcgatgaac tgcgttcagg caaactcgac gttttctacg atctctacaa tctggcgcaa
     8941 aagcgccgtt ttgagcgtta ccagtacgct ttgtcggtac tggaaaagcc gatggatttc
     9001 accggcaacg acacttataa ccttgaccgc agcaaagcgc cctggccgaa aaacgaggct
     9061 gagttgaacg cgctgtggga cagtaaagtc aaattcgacg agttaagcct gaagctggca
     9121 ggaaaaacgg ataaagaaat tcgtgaaacc ctgacacgcc gctacaaatt tgccattcgt
     9181 cgtctggcgc aaaccaacag cgaagatgtt ttctcgctgg caatgacggc gtttgcgcgt
     9241 gaaatcgacc cgcataccaa ctatctttcc ccgcgtaata ccgaacagtt caacactgaa
     9301 atgagtttgt cgctggaagg tattggcgca gtgctgcaaa tggatgatga ctacaccgtt
     9361 atcaattcga tggtggcagg tggtccggca gcgaagagta aagctatcag cgttggtgac
     9421 aaaattgtcg gtgttggtca aacaggcaag ccgatggttg acgtgattgg ctggcgtctt
     9481 gatgatgtgg ttgccttaat taaagggccg aagggcagta aagttcgtct ggaaatttta
     9541 cctgctggta aagggaccaa gacccgcact gtaacattga cccgtgaacg tattcgtctc
     9601 gaagaccgcg cggttaaaat gtcggtgaag accgtcggta aagagaaagt cggcgtgctg
     9661 gatattccgg gcttctatgt gggtttgaca gacgatgtca aagttcaact gcagaaactg
     9721 gaaaaacaga atgtcagcag tgtgatcatc gacctgcgta gcaatggcgg tggggcgttg
     9781 actgaagcgg tatcgctctc aggtctgttt attcctgcgg gtcccattgt tcaggtccgc
     9841 gataacaacg gcaaggttcg tgaagacagc gataccgacg ggcaggtgtt ctataaaggc
     9901 ccgctggtgg tactggttga ccgtttcagt gcttcggctt cagaaatctt tgccgcggca
     9961 atgcaggatt acggtcgtgc gctggttgtg ggtgaaccga cgtttggtaa aggcaccgtt
    10021 cagcaatacc gttcattgaa ccgtatttac gatcagatgt tacgtcctga atggccagcg
    10081 ctgggttctg tgcagtacac gatccagaaa ttctatcgcg ttaacggcgg cagtacgcaa
    10141 cgtaaaggcg taacgccaga catcatcatg ccgacgggta atgaagaaac ggaaacgggt
    10201 gagaaattcg aagataacgc gctgccgtgg gatagcattg atgccgcgac ttatgtgaaa
    10261 tcaggcgatt taacggcctt tgggccggag ctgctgaagg aacataatgc gcgtatcgcg
    10321 aaagatcctg agttccagaa catcatgaag gatatcgcgc gcttcaacgc tatgaaggac
    10381 aagcgcaata tcgtttctct gaactacgct gtgcgtgaga aagagaataa tgaagatgat
    10441 gcgacgcgtc tggcgcgttt gaacgaacgc tttaaacgcg aaggtaaacc ggagttgaag
    10501 aagctggatg atctaccgaa agattaccaa gagccggatc cttatctgga tgagacggtg
    10561 aatatcgcac tcgatctggc gaagcttgaa aaagccagac ccgcggaaca acccgctccc
    10621 gtcaagtaat atcaatcagg cacaagaaat tgtgcctgat tttttaacag cggcaagatg
    10681 ccgtaaatca gatgctacaa aatgtaaagt tgtgtctttc tggtgactta cgcactatcc
    10741 agacttgaaa atagtcgcgt aacccatacg atgtgggtat cgcatattgc gttttgttaa
    10801 actgaggtaa aaagaaaatt atgatgcgaa tcgcgctctt cctgctaacg aacctggccg
    10861 taatggtcgt tttcgggctg gtactgagcc tgacagggat acagtcgagc agcgttcagg
    10921 ggctgatgat catggccttg ctgttcggtt ttggtggttc cttcgtttcg cttctgatgt
    10981 ccaaatggat ggcattacga tctgttggcg gggaagtgat cgagcaaccg cgtaacgaaa
    11041 gggaacgttg gctggtcaat actgtagcaa cccaggctcg tcaggcaggg atcgctatgc
    11101 cgcaagtggc tatctaccat gcgccggaca tcaacgcttt tgcaaccggt gcgcgccgtg
    11161 atgcctctct ggttgctgtc agcaccggtt tgctgcagaa catgagcccg gatgaagccg
    11221 aggcggtaat tgctcacgaa atcagccaca tcgccaatgg tgatatggtc accatgacgc
    11281 tgattcaggg cgtggtgaac accttcgtta tctttatttc ccgtattctg gcgcagcttg
    11341 ccgcgggttt tatgggcgga aatcgtgatg aaggtgaaga gagcaacggc aacccgctga
    11401 tctactttgc ggttgcaacg gttctggaac tggtgtttgg tattctggcg agcattatca
    11461 ccatgtggtt ctcgcgtcat cgtgaattcc atgctgatgc cggttcggca aaactggttg
    11521 gtcgcgagaa aatgattgcc gcgctgcagc gcctgaaaac cagctatgaa ccgcaagaag
    11581 caaccagcat gatggctttc tgcattaatg gtaagtcgaa atcgctcagt gagttgttca
    11641 tgactcaccc gccgctggat aaacgaattg aagctctgcg tacgggtgaa tacctgaagt
    11701 aatatgaagt aaatactaaa aagtgcgtcc tgatcg
//
LOCUS       DAJVOY010000125        11652 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVOY010000125 DAJVOY010000000
VERSION     DAJVOY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.125"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..588)
                     /gene="nfeF"
                     /locus_tag="OQL90_002952"
     CDS             complement(<1..588)
                     /gene="nfeF"
                     /locus_tag="OQL90_002952"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCS1369657.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWL"
     gene            876..1499
                     /gene="nfeR"
                     /locus_tag="OQL90_002953"
                     /gene_synonym="yqjI"
     CDS             876..1499
                     /gene="nfeR"
                     /locus_tag="OQL90_002953"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1369658.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(1653..3173)
                     /gene="aer"
                     /locus_tag="OQL90_002954"
     CDS             complement(1653..3173)
                     /gene="aer"
                     /locus_tag="OQL90_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCS1369659.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDVSADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            3590..4969
                     /gene="ygjG"
                     /locus_tag="OQL90_002955"
     CDS             3590..4969
                     /gene="ygjG"
                     /locus_tag="OQL90_002955"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1369660.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLLAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5011..5343)
                     /locus_tag="OQL90_002956"
     CDS             complement(5011..5343)
                     /locus_tag="OQL90_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCS1369661.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            5562..6545
                     /gene="ebgR"
                     /locus_tag="OQL90_002957"
     CDS             5562..6545
                     /gene="ebgR"
                     /locus_tag="OQL90_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1369662.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            6729..9821
                     /gene="ebgA"
                     /locus_tag="OQL90_002958"
     CDS             6729..9821
                     /gene="ebgA"
                     /locus_tag="OQL90_002958"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCS1369663.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLGNLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            9818..10267
                     /locus_tag="OQL90_002959"
     CDS             9818..10267
                     /locus_tag="OQL90_002959"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1369664.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            10330..>11652
                     /locus_tag="OQL90_002960"
     CDS             10330..>11652
                     /locus_tag="OQL90_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1369665.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVG"
BASE COUNT         2789 a         3140 c         2953 g         2770 t
ORIGIN      
        1 cagccattcg atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg
       61 cacgctaacc agcgcactaa cttgcggttt aacggcaagt ttgctcaacg tttccaggcg
      121 gcggcgcagt gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcttc
      181 cggcaccacc agcgaaccgc gcggaccagc caccgtaagt ttatcgcccg gttgcgcctg
      241 catcgcccag ccgctggcga ccccaccgtc gtgaataaag aaatcgatcg ccagttcatg
      301 gcgtagttcg tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc cttccggcca
      361 gacgatgccc tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa
      421 gagtttgctg tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct cgccgccgag
      481 gacaatgcgc tgaaaaccgg cgctgatgcg ctcaacgcgt aacacagtca gttcacggaa
      541 gcgcagatca ttgcgaacgc gctgcgggta gcggggggag ttattcattg ttatcgcctt
      601 cgtgatggta atcagatata tctaaataat tttcgtaaat gataatgatt gttaatcatg
      661 ataaatgcaa gcgattagta gaactgatat gtctatagtc tgataagacg aaccgcctct
      721 tctcaggcat cattactcaa cgccggatgc ggcgtgaacg ccttatccgg cctacgtgtg
      781 agatgagtcc acatttaaaa atcattttta ttcttgcaag agtgctcata tcagatataa
      841 attagatata tctaatcaag aaaaaggagg ctgatatgag ccatcatcac gaagggtgtt
      901 gtaaacatga aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg
      961 agcactgcgg acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc
     1021 gcggcggcgg tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg
     1081 atattctctc gcgcgatgac agccacggtt acgaattgat taaagcgatt gagaatctaa
     1141 cccaggggaa ttacacccca agcccgggcg tcatctaccc aacgctggat tttctgcagg
     1201 agcagtcgct gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg
     1261 aacaaggcgc gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca
     1321 tcaaagcgcg ttgcgttggc gcggcgctgc gccagaaccc gcaaatgaag cgggcgctgg
     1381 ataattttaa agcggtgctg gatttacgcg tcaaccagag cgatatcagt gatgcacaaa
     1441 taaaaaagat cattgcggtg atcgaccgcg ccgcttttga tattacgcaa ctggattaat
     1501 cgccgcatcc gccagtggcg cggtgcaatt gccggatgcg atgcttgacg cgccttatcc
     1561 ggcctacacc cactacacac cccgcaggcc tgataagatg cgccagcatc gcatcaggca
     1621 ttgtgctcca accgccggat ccggcatacc gattaatgca gcaccgtcac cgcgtcttcc
     1681 agtcggctgg cgcggtgttt caccatcgcc gacacctgcg cactctcttc caccagctca
     1741 gcatttttct gggtgatcag gttaagctca tccactgcac gggtcaggct ggaaagccca
     1801 tcggcctgtt ccagcgttga atggctaatc tgggcgatca actgggtgac gtttttcacc
     1861 tgtgccacaa tatcttccat cgtccgtccg gcggcgtgta cctgctgcga accggattgc
     1921 accttatcag cactgacatc aatcagcttg cgaatatcgt tggcagcatt agcgctgcgg
     1981 ctggctaaat gacgcacttc ccctgccacc actgcaaaac ctttgccctg ttcgccggca
     2041 cgcgccgctt ccaccgccgc attcagggcc agaatattgg tctgaaacgc aatatcgtta
     2101 atcagcgaag taatggtgcc aatgcgctgg gtactgtcgg cgatatcgtc cattgtcttg
     2161 atcaccgtgg tcatcgcctc cccaccctgc actgccgcat tactggcgat gatagaaagt
     2221 ttatcggcag ccgacgccgt ggcagagttc tgtttcaccg acgccgccat ttggttcatg
     2281 gtcgccaccg tttgctgaac gttatcaact gtctgctggg tatgttcgtt cagttcatcg
     2341 gtgcctttcg ccagcgtctc actgccattt ctgacactgg acacctggct tgagacatcg
     2401 ttaattagcc aacggcacat caggccaagc tgccctaccg cgcgtaatgt cagccccagc
     2461 tcatcgctgc gattcaggtg ctcaacacta ttacgctctc cggtcgccac cttcagtgcc
     2521 tgacgggcga cattttctat cgggcgcaca atctgccatt caaaacaagc gcttgccaac
     2581 aataccacta acacacagag gaaatacgtc accaccgggg cagcaacaaa ccaaagcatg
     2641 gccgccagca agataaacat cagggtcatc actccacgcg cccgccagcg aagcggtaat
     2701 gaaggcagtt tacccagcca gcctttacgc accaccaggc ctttatgaat acgcttactg
     2761 gtacgtccgg cgttcagcgc tttgtacagc ggctccaccg ccgcaatctc ttcatccgtc
     2821 gcccgggtac gaatcgacat atagccactg atttttccct cgcgcaccat cggtaccgca
     2881 ttggcccgca cccaataatg gtcgccattt ttgcggcgat ttttcacgat gccgctccag
     2941 ggctcccctt ttttcagggt gaaccacata tccgcaaacg ccgcttttgg catatccggg
     3001 tgacgcacca tgttgtgcgg ctgcccttgt aactcttgca aggtaaagcc gctcacctgc
     3061 acaaaagtgt cattagcatg agtgatatag ctttgcagat cggtagtgga catcagagtg
     3121 gtatcgtccg ccagcggggt attttgctgg gtgacatacg gatgagaaga catgttcgcg
     3181 tcctatgcag gttatatggt tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg
     3241 ttatctttaa ccgattaatt tgatttagat cgcaatttgc gattaaagca caaatctaat
     3301 tccttgattt aaaatacttt cactctgtta ctatacgaaa acgttaatta tcttgcccaa
     3361 aatcaggcaa ttatcgccct gaaaacgtgc atttgcacag caatcatcaa atccataccc
     3421 gacaaaaacc gtgcaaaata acaacgaatg ttaacaaata gcattaaata ctgcgcaaat
     3481 gataaccaca tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct
     3541 gcaatactta aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt
     3601 accttcgagc gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg
     3661 aacgctggat catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga
     3721 gcatgtcaat ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg
     3781 agccgtagag tggcaagcgg gaagtttaaa tacgcttgtc gacacccagg gacaggagtt
     3841 tatcgactgc ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt
     3901 ttccgccgta cagaatcaac ttgcgaaaca accgctgcac agccaggagc tgctcgatcc
     3961 gttacgggcg atgttggcga aaacccttgc tgcgctaacg cccggtaaac tgaaatacag
     4021 cttcttctgt aatagcggca ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta
     4081 ccagtcaccg cgcggcaagt ttacttttat tgccaccagc ggcgcgttcc acggtaaatc
     4141 acttggcgcg ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgccgttact
     4201 gccgggcttc cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga
     4261 gtgcaaaaaa accggtgatg atgtggctgc ggtgatcctc gaaccgattc agggtgaagg
     4321 tggcgtaatt ctgccgccgc cgggctatct caccgccgta cgtaagctat gcgatgagtt
     4381 cggcgcactg atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt
     4441 cgcctgcgag catgagaacg tacagccgga tatcctctgc cttgccaaag cgctcggcgg
     4501 cggcgtgatg ccgattggcg cgaccatcgc cactgaagag gtgttttcag ttctgttcga
     4561 caacccattc ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct
     4621 ggcgaccatc aatgtgttgc tggagcagaa cttactggct caggctgagc aaaaaggcga
     4681 tatgttgctg gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc
     4741 gcgtggtaaa gggatgttga tggcaattga gtttgttgat aacgaaatcg gctataactt
     4801 tgccagcgag atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac
     4861 gatccgcatt gaaccgccac tgacactgac cattgaacag tgtgaactgg tgatcaaagc
     4921 ggcgcgtaag gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg
     4981 atggcgatac gacgtcgcca tccgatcttt ttatacgacg cgtacgcccg caggcatcat
     5041 ccgctccggc gttaacaaca cgctttcact gccgtcatcg gtttccgcac acaacagcat
     5101 gcattccgac gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt
     5161 tttccccatc agctcttctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt
     5221 tttttccccc acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac
     5281 ttccacaatc tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc
     5341 cattgtttcc ttccttcggg taaaatttta ctaaactata gcaaaagttt ttctcaatcc
     5401 tgtaggctaa aaatggagaa tgcaggcgtg atcacattcg tgagccgctg tgttaccgtt
     5461 acagcgtcaa agaaacgcgc tttatttact gaaaacaggt gacccgataa gtacttcctc
     5521 tacaatgggg gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca
     5581 tcgcaatcga agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga
     5641 cattgaatgt gaaagaagag acgaaacatc gcattctcga gatcgccgaa aagctggagt
     5701 acaagaccag tagtgcccgc aaactccaga caggtgcagt caaccaacac catattctgg
     5761 ctatctacag ctaccaacag gagctggaga tcaacgatcc ttactatctg gcgatccgcc
     5821 acggcattga aactcagtgc gaaaagctgg gcattgagct caccaactgt tatgaacaca
     5881 gcggattacc agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg
     5941 cccttcgcgc cgccgcctgt gcgttgaccg acaatatctg ttttatcgac tttcacgaac
     6001 ccggcagcgg ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg
     6061 acttctatat caatcagggc gttaatcgta ttggttttat tggcggtgaa gatgagccag
     6121 gcaaagcgga tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac
     6181 gcgaagagga tatctggcgc ggaggctttt ccagttcgtc gggttatgaa ctggcaaaac
     6241 agatgctggc gcaggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta
     6301 tcggcgtact gcgggcaatt catgaacgag gcctgaacat cccacaggat atttcgctta
     6361 tcagcgttaa cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca
     6421 tccattccga gatgatggga agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg
     6481 gtcgcgcgct gccgctgtta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc
     6541 gttaaatccc cttacacact gtccggcaat cgtttttgcc ggacagtgct gccgtttatt
     6601 ttcgtgatcc agttaaagta aatgcattta tctgctactt tttagtaaaa attttactga
     6661 actccccagc aattacacaa actaccatca ccatgaatgg ttccgatttc tctctaccgg
     6721 gaggccctat gaatcgctgg gaaaacattc agctcaccca cgaaaaccga cttgcgccgc
     6781 gtgcgtactt tttttcatat gattctgttg cgcaagcgcg tacctttgcc cgcgaaacca
     6841 gcagcctgtt tctgccctta agcggtcagt ggaatttcca cttttttgac catccgctgc
     6901 aagtaccaga agccttcacc tctgagttaa tggctgactg ggggcatatt accgtccccg
     6961 ccatgtggca aatggaaggt cacggcaaac tgcaatatac cgacgaaggt tttccgttcc
     7021 ccatcgatgt gccgtttgtc cccagcgata acccaaccgg tgcctatcaa cgtattttca
     7081 ccctcagcga cggctggcag ggtaaacaga cgctgattaa atttgacggc gtcgaaacct
     7141 attttgaagt ctatgttaac ggtcagtatg tgggtttcag caagggcagt cgcctgaccg
     7201 cagagtttga catcagcgcg atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga
     7261 tgcagtgggc ggactctacc tacgtggaag accaggatat gtggtggtca gcggggatct
     7321 tccgcgatgt ttatctggtc ggaaaacacc taacgcatat taacgatttc actgtgcgta
     7381 ccgactttga cgaagcctat tgcgatgcca cgctttcctg cgaagtggtg ctgggaaatc
     7441 tcgccgcctc ccctgtcgtc acgacgctgg aatataccct gtttgatggc gaacgcgtgg
     7501 tgcacagcag cgccattgat catttggcaa ttgaaaaact gaccagcgcc agctttgctt
     7561 ttactgtcga acagccgcag caatggtcag cagaatcccc ttatctttac catctggtca
     7621 tgacgctgaa agacgccaac ggcaacgttc tggaagtggt gccacaacgc gttggcttcc
     7681 gtgatatcaa agtgcgcgac ggtctgttct ggatcaataa ccgttatgtg atgctgcacg
     7741 gcgtcaaccg tcacgacaac gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg
     7801 agaaagatct ccagttgatg aagcagcaca atatcaactc cgtgcgtacc gctcactacc
     7861 cgaacgatcc gcgtttttac gaactgtgtg atatctacgg cctgtttgtg atggcggaaa
     7921 ccgacgtcga atcgcacggc tttgctaatg tcggcgatat cagccgtatt accgacgatc
     7981 cgcagtggga aaaagtctac gtcgagcgca ttgttcgcca tatccacgcg cagaaaaacc
     8041 atccgtcgat catcatctgg tcgctgggca atgaatccgg ctatggctgt aacatccgcg
     8101 cgatgtacca tgcggcgaaa gcgctggatg acacgcgact ggtgcattac gaagaagatc
     8161 gcgatgctga agtggtcgat attatttcca ccatgtacac ccgcgtgccg ctgatgaatg
     8221 agtttggtga atacccgcat ccgaagccgc gcatcatctg tgaatatgct catgcgatgg
     8281 ggaacggacc gggcgggctg acggagtacc agaacgtctt ctataagcac gattgcattc
     8341 agggtcatta tgtctgggag tggtgcgacc acgggatcca ggcacaggac gaccacggca
     8401 atgtctggta taaattcggc ggcgactacg gcgactatcc caacaactat aacttctgtc
     8461 ttgatggttt gatctattcc gatcagacgc cgggaccggg cctgaaagag tacaaacagg
     8521 ttatcgcgcc ggtaaaaatc cacgcgcggg atctgactcg cggcgagttg aaagtcgaaa
     8581 ataaactgtg gtttaccacg cttgatgact acaccctgca cgcagaggtg cgcgccgaag
     8641 gtgaaacgct cgcgacgcag cagattaaac tgcgcgacgt tgcgccgaac agcgaagccc
     8701 ccttgcagat cacgctgccg cagctggacg cccgcgaagc gttcctcaac attacggtga
     8761 ccaaagattc ccgcacccgc tacagcgaag ccgggcattc tatcgccact tatcagttcc
     8821 cgctgaagga aaacaccgcg cagccagtgc ctttcgcacc aaataatgcg cgtccgctga
     8881 cgctggaaga cgatcgtttg agctgcaccg ttcgcggcta caacttcgcg atcaccttct
     8941 caaaaatgag tggcaaaccg acatcctggc aggtaaatgg cgagtcgctg ctgacccgcg
     9001 agccaaagat caacttcttc aagccgatga tcgacaacca caagcaggag tacgaagggt
     9061 tgtggcagcc gaatcatttg cagatcatgc aggaacatct gcgcgacttt gccgtagaac
     9121 agagcgatgg tgaagtgttg atcatcagcc gcacagttat tgcaccgccg gtgtttgact
     9181 tcgggatgcg ctgtacctac atctggcgca tcgctgccga tggccaggtt aacgtggcgc
     9241 tttccggcga gcgttacggc gactatccgc acatcattcc gtgcatcggt ttcaccatgg
     9301 ggattaacgg cgaatacgat caggtggcgt attacggtcg tggaccgggc gaaaactacg
     9361 ccgacagcca gcaggctaac atcatcgata tctggcgcag caccgtcgat gccatgttcg
     9421 agaactatcc cttcccgcag aacaacggca accgtcagca tgtccgctgg acggcactga
     9481 ctaaccgcca cggcaacggt ctgctggtgg ttccgcagcg cccaattaac ttcagcgcct
     9541 ggcactatac ccaggaaaac atccacgctg cccagcactg taacgagctg cagcgcagtg
     9601 atgacatcac cctgaacctc gaccaccagc tacttggcct cggctccaac tcctggggca
     9661 gcgaggtgct ggactcctgg cgcgtctggt tccgtgactt cagctacggc tttacgttgc
     9721 tgccggtttc tggcggagaa gctaccgcgc aaagtctggc gtcgtatgag ttcggcgcag
     9781 ggttcttttc cacgaatttg cacagcgaga ataagcaatg aggatcatcg ataacttaga
     9841 acagttccgc cagatttacg cctctagcaa gaagtggcaa cgctgcgttg aagcgattga
     9901 aaatatcgac aacattcagc ctggcgtcgc ccactccatc ggtgactcat tgacttaccg
     9961 cgtggagaca gactccgcga ccgatgcgct atttaccggg catcgacgct attttgaagt
    10021 gcattactac ctgcaagggc agcaaaaaat tgaatatgcg ccgaaagaga cattacaggt
    10081 agtggaatat tatcgtgatg aaactgaccg tgaatattta aaaggctgcg gagaaaccgt
    10141 tgaggtccac gaagggcaaa tcgttatttg cgatatccat gaagcgtatc ggtttatctg
    10201 caataacgcg gtcaaaaaag tggttctcaa agtcaccatc gaagatggtt atttccataa
    10261 caaataacaa ctacggcggc aaaaggagtt tgccgccacc gctaccctac acattttcgg
    10321 agatgtgtta tgtctgatac caaacgtaat acaatcggca aattcggctt gctctcgctg
    10381 acttttgctg ccgtttacag ctttaacaac gttatcaata ataatattga gcttggactg
    10441 gcctcggcac cgatgttttt cctcgcgacg attttttatt ttattccctt ctgtctgatc
    10501 atcgcagaat ttgtttcgtt aaataaaaac tcagaagccg gtgtctacgc gtgggtaaaa
    10561 agttcgctgg gcggacgttg ggcatttatt actgcctata cctactggtt cgtaaacctg
    10621 ttctttttca cctcactgtt gccgcgcgtt attgcttatg cttcgtatgc cttcctcgga
    10681 tacgaatata ttatgacgcc ggttgccacc accattatca gtatggtgct gttcgccttc
    10741 tccacctggg tttccaccaa cggggcgaaa atgttggggc caattacctc cgtcacttca
    10801 acgctgatgc tgctgttaac gctctcctac attttactgg caggtacggc gctggttggc
    10861 ggcgtacagc ctgctgaccc catcaccgtt gacgcgatga tcccgaactt caactgggcg
    10921 ttcctcggcg ttaccacctg gatctttatg gccgcaggtg gcgcggagtc cgtcgctgtg
    10981 tacgttaacg acgtcaaagg cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg
    11041 atttttatcg gcgtactgta ttccgtctcc tcggtgctga ttaacgtctt cgtcagcagc
    11101 aaagagttga aatttaccgg cggatcggtg caggtattcc atggcatggc ggcgtatttt
    11161 ggtctaccgg aagcgttgat gaatcgcttt gtcggtctgg tgtcctttac cgcgatgttc
    11221 ggttccctgc tgatgtggac cgcaacgccg gtgaaaattt tcttctccga aatcccggaa
    11281 ggcatctttg gtaagaaaac cgtcgaactg aacgaaaacg gcgttccggc gcgcgcagcg
    11341 tggatccagt tcctgatcgt catcccgctg atgattatcc cgatgctcgg ttccaatacc
    11401 gtgcaggatc tgatgaatac aattattaat atgaccgccg cagcgtccat gcttccgccg
    11461 ttattcatca tgctggctta cctgaattta cgcgccaaat tagatcacct gccacgcgat
    11521 ttccgtatgg gctcccgccg caccggtatt atcgttgttt caatgctgat tgcgatattt
    11581 gccgtagggt ttgtcgcttc gacattcccg actggcgcga atattctgac catcattttt
    11641 tataacgtcg gc
//
LOCUS       DAJVOY010000126        11625 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVOY010000126 DAJVOY010000000
VERSION     DAJVOY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.126"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(51..1037)
                     /locus_tag="OQL90_002961"
                     /pseudo
     CDS             complement(51..1037)
                     /locus_tag="OQL90_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(1246..1905)
                     /locus_tag="OQL90_002962"
     CDS             complement(1246..1905)
                     /locus_tag="OQL90_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1369666.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2232..2588)
                     /locus_tag="OQL90_002963"
     CDS             complement(2232..2588)
                     /locus_tag="OQL90_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1369667.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2655..2949)
                     /gene="yebG"
                     /locus_tag="OQL90_002964"
                     /pseudo
     CDS             complement(2655..2949)
                     /gene="yebG"
                     /locus_tag="OQL90_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3083..4261
                     /gene="purT"
                     /locus_tag="OQL90_002965"
     CDS             3083..4261
                     /gene="purT"
                     /locus_tag="OQL90_002965"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1369668.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4317..4958)
                     /gene="kdgA"
                     /locus_tag="OQL90_002966"
                     /gene_synonym="eda"
     CDS             complement(4317..4958)
                     /gene="kdgA"
                     /locus_tag="OQL90_002966"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1369669.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4995..6806)
                     /gene="edd"
                     /locus_tag="OQL90_002967"
     CDS             complement(4995..6806)
                     /gene="edd"
                     /locus_tag="OQL90_002967"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1369670.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRRFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(7041..8516)
                     /gene="zwf"
                     /locus_tag="OQL90_002968"
     CDS             complement(7041..8516)
                     /gene="zwf"
                     /locus_tag="OQL90_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1369671.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8853..9722
                     /gene="hexR"
                     /locus_tag="OQL90_002969"
                     /gene_synonym="yebK"
     CDS             8853..9722
                     /gene="hexR"
                     /locus_tag="OQL90_002969"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1369672.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9850..11292
                     /gene="pyk"
                     /locus_tag="OQL90_002970"
     CDS             9850..11292
                     /gene="pyk"
                     /locus_tag="OQL90_002970"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1369673.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11352..11570)
                     /locus_tag="OQL90_002971"
     CDS             complement(11352..11570)
                     /locus_tag="OQL90_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369674.1"
                     /translation="MEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGM
                     RRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         2819 a         2993 c         2904 g         2909 t
ORIGIN      
        1 ctgctcaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aaccagccag
       61 tcggtaaaca gcgtaaaccc ttccagcatg atgttttcgc gtggcggaat taactcttcc
      121 cactgttgct catcacgcag gcgggtacgg tataagccaa agtttttgcc gtggcggttg
      181 gaacgcagat aaaaccgatg ctggtagtga tcaaggctgt attcgtgatc tttgcggcgc
      241 ggcagaaaaa caaacggctc ggcatcggcc atttccgcgt ccagcaggcg aacttcactg
      301 gtggtggcgc tggccaaatg aatgactaca tagtgcttcg aggtcgtttt atgcaggctg
      361 acgtaatagg tatcgtcttt ttcttcgtag atcagtttat cttgcgatgc tggcgtaccg
      421 atggcgtgac gccagacctg ataaggcagc agcgtcaccg gatgcttgcg aacatagtag
      481 aaagtccagg agtcatttgc ccagacaaag ctgggttcaa cgttatccag cagttccggg
      541 taccagttac cagtttccag attacgaaaa cgaatgccgt actggcgtcg ggaaagaaaa
      601 tcttctgcca gcgccataat ggtgttatcg ggcgtaatcg ccattccgcc catcgaataa
      661 aactcactat gagctgcgcg cttattggca tcgagcaatg tttcccactc atcccactct
      721 tcactgaatg ccgattgacg ctggtagata gcatattcac agcctggttc ataaatatgc
      781 cgatagcggt agccattttt gatgtagggc gcagaaactt ctcgttgcgg aatgcggtcg
      841 atgatttcct ttaagatgcg atcctgcaag gcttgttgtg aggccatcac ccgatgaccg
      901 taactatttt cttgttgcag gtagtccagg acttctggct gagaacgcgt atcgtcccgc
      961 agccagtagt aattatcgat gcgcgtatcg ccatgaagcg tcatggcgtg gggaatgcgg
     1021 gcggcttttg gtagcatgtt attgttcttt ctggttgaaa catcttataa gggtggcaaa
     1081 actcaccggg gatgcaagcg aaacagggga gtcattgctt agatgatgac aggtaatggc
     1141 gcggatatcg aatgttatgc agacagagaa aatcagcctg ttcaaatggc tgtgcgattc
     1201 tggatagccc gaaatagtca acttcaggct atccagagag cggaattatt ccgccaaagt
     1261 gcgtttttgc tgttcgagat cgcgttcaat gccgtcacga acatcctggg ggattttcag
     1321 cgcgtcaccc agtgcattca ggtaactgcg ttccataaaa tggtcaatat caatagccgc
     1381 gcaactcagg aaatagattt ccagcgcctc ttcttcattg cggaccccgg tagccaagcg
     1441 ttgtggatcc agcggttgtt cgattgcctg ctcaatgagt acacgcccct gctcttccac
     1501 gccggcttca cgcaattgct ggtcgattgc cgcacgttct ttggcatcaa tatgaccatc
     1561 acttttagcg gcaaagacca gcgcaaggat caaacgttct gtacgctcat ccagcggcgt
     1621 actttgcgcg ccaaactgcg gttcgtcctg atgcgccgcg cgaattttat ctttgtattt
     1681 attccacagc accgtacccg ctaccgctcc gccgccaacc agtaacgcgt tggtgccata
     1741 tttcgtaaga agtttacgtg ctgatttatt tgcgaccagc agcccagcca gtccgcctaa
     1801 tgcgcctggc actaacagtt tgcccaatcc ctgatccgca gacgaggaga tagaagaact
     1861 gctttgccca agcagggatt gcagttgatt taaccagtta gccatatttg ctcctcaata
     1921 accattttta gtgcctgtag catagcggat gaggatgtca gaaaatgtat atcgagacaa
     1981 aagatgcgca aataagcggg gcggtaggcc gttattcaaa gaaattcgcg ccaggtaagg
     2041 tatttactgt atatcaggtg ataagggtat tttaggtgaa agtagtttgt cgttttgagt
     2101 ggcattgttt gatgctgatt gcccgatgcg acgctgttgc gtcttctcag gcctacgatt
     2161 gtggtgcgtt ctggggcgga caaggcactc gcgccgcatc cgccaacggt caatcgaatt
     2221 atcagaagcc attaacgccg ctgatattcc gccattcccg ctttgcagtc cacgctgacc
     2281 tggtaatgaa tatcggcact tttaccacgc acggttagcg gtactgacca tttatcatct
     2341 ttaccctgaa tgtcctgcaa actgacccaa gccacgggat cggcctgacc gacaattttt
     2401 tgatcatctg cccaacgcgc cacgcgattt tgttgataat cacgttttac gctcgcggca
     2461 attccggcag catccagacc ttcacacttt gggaaagtga ccgacttgct ggtttcatta
     2521 ttggcagcga aaactgatgc gcaggcagaa accaacaaca gccctaaaaa cgcccctctt
     2581 tttttcatgt ttttctccat agcacaatga ttcaggagaa agcatggtac aaattgtcag
     2641 gagcgcaagt tgcttcaggc cgcgtgtgag gcatcttcct cttcggattc agcaccgacg
     2701 acctgtgggt ggggacggta atttgccggt tttcagaatg gtgctcagca catctttttg
     2761 ttctgccagc catagcgaaa gggcttcacg ttgctcgtct tccatttgca ctggagaatt
     2821 agtcagccag gtgtcaagca gatccgccgt atcaagcatt ttgtcataag catcggcttc
     2881 ctttttgctg gtaaacgaca ttttctcttc gccctcacga atgactacgt atttaacttc
     2941 aaccgccatt tgcagcctct cataataact gtgattttat acagtgtatt tcttttcggt
     3001 tgagaaatca aaatcaacaa taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg
     3061 aattaatcag gggatattcg ttatgacgtt attaggcact gcgctgcgtc cggcagcaac
     3121 tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg
     3181 tctcggcgta gaggtgattg ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc
     3241 gcatcgctcc catgtcatta atatgcttga tggtgatgca ttacgccgtg tggttgaact
     3301 ggaaaaacca cattatatcg tgccggagat cgaagctatt gccaccgata tgctgatcca
     3361 acttgaagag gaaggactga atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa
     3421 tcgcgagggt atccgtcgcc tggcggcaga agagctgcag ctgcccactt ccacttatcg
     3481 ttttgccgat agcgaaagcc ttttccgcga ggcggttgct gacattggct atccctgcat
     3541 tgtaaaaccg gtgatgagct cttccggcaa ggggcagacg tttattcgtt ctgcagagca
     3601 acttgctcag gcatggaagt acgctcagca aggcggtcgc gccggagcgg gccgcgtaat
     3661 tgttgaaggc gtcgttaagt ttgacttcga aattaccttg ctaaccgtca gcgcggtgga
     3721 tggcgtccat ttctgtgcac cggtaggtca tcgccaggaa gatggcgact accgtgaatc
     3781 ctggcaacca cagcaaatga gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa
     3841 agtggtgctg gcactgggcg gttatggtct gtttggtgtc gagctgtttg tctgtagtga
     3901 tgaggtgatt ttcagtgagg tctcccctcg tccacatgat accgggatgg tgacgttaat
     3961 ttctcaagat ctttcagagt ttgccctgca tgtacgtgcc ttcctcggac ttccggttgg
     4021 cgggatccgt cagtatggtc ctgcagcttc tgccgttatt ctgccacaac tgaccagtca
     4081 gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt
     4141 tggtaagccg gaaattgatg gcagccgtcg tctgggtgtt gcgctggcga ccgctgagag
     4201 tgttgttgac gccattgaac gcgcgaagca cgccgccgga caggtaaaag tacagggtta
     4261 aacccggcaa aaaaacgcta caaaaatgcc cgatcgtaga tcgggcgttt tgacttttac
     4321 agcttagcgc cttctacagc ttcacgcgcc agcttagtaa tgcggtcgta atcgcccgct
     4381 tccagcgcat ctgccggaac cagccaggaa ccaccgatgc acagcacgct tttcagcgcc
     4441 aggtagtcac ggtagttagc cggagaaata ccacccgtcg ggcagaaacg gacctgggag
     4501 aacggacccg cgatcgcctg cagggctttc acgccgccgt tagcttcagc cgggaagaat
     4561 ttgaactctt tcaaaccgta gtccataccc agcatcagtt cggaaacagt gctgatcccc
     4621 ggaattagag gaatagtccc ttcggtagca gctttcagca gcggctcggt cagacccggg
     4681 ctaattgcga actgtgcacc cgcttcagtg acttctgcca gctgctgtgg attcagcacc
     4741 gtaccggcac ccacaatcgc ttcaggcact tctttggcga tagcacggat agcgtcaact
     4801 gcacactcgg tacgcagagt cacttccaga acgcgcaccc caccagcaac caacgctttt
     4861 gccatcggca ctgcgtgttc cagttttttt accacgataa ccggtacaac cgggccggtg
     4921 gtcaggattg attctgcact tgttttccag tttttcatca gagttttctc tcgcctgatt
     4981 acaaatttgt cgtcttaaaa agtgatacag gttgcgccct gttcggcacc ggacagtttt
     5041 tcacgcaagg cgctgaataa ttcacgtcct gtccccacgc gtgacgcgct caggtcagga
     5101 atgtgcggtt cgcgagcagc cagttccgct tcgtctacca gcagcgtcag ttcgcctgtc
     5161 tgtccattca cacgaatgat gtccccgtcg cgcacttttg ccagcagccc gccatcgtag
     5221 gcttctggtg ttacgtggat agctgacggc actttacctg aagcgccgga gagtcgtcca
     5281 tcggtaacta acgcaatttt gaaacgccgg tccaataata caccaagtgg cggcatgagt
     5341 ttatgtaatt ctggcattcc gttcgctttt ggcccctgat gacggacaac aacgacacaa
     5401 tcgcggtcca gcaaacccgc ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca
     5461 accgctggcg cttcaatcac ctggttctca accggcacgg cagaggtttt cataaccgca
     5521 cggcccaggt taccgcttaa cacttttgtc ccaccatgat gagagaaagg ttgttcgaag
     5581 gaagcgatca cattgctgtc gagtgatttt tccgcccctt cccgccagtc cagttcacca
     5641 ttattcagcc atggttcaag ggtataacga gacagaccaa agcctgccac cgtattgaca
     5701 tcttcatgca gcaggcctgc tttgagcagt tcacgcacca gaaccggtac gccacctgcc
     5761 gcctggaagt ggttaatatc ggccggaccg ttcgggtaga gacgtgccat cagcggtaca
     5821 acatcagaaa ggtcagagaa gtcatcccag ttaatctgaa taccggccgc gcgcgccatc
     5881 gccaccaggt gcatggtgtg gttagtggaa ccaccggtcg ccagcagtgc aacgataccg
     5941 ttcaccacca ctttctcatc gatcatctta ccgatcggca tccattcatt accattaccg
     6001 gtcatgcgtg taacctgacg cgcagcggca gcggtcaaag catcgcgcag cggagaatcc
     6061 ggatgaacaa aagaagagcc tggcgactgc atccccataa attccaccac catctggttg
     6121 gtgttggcag taccgtagaa agtacatgtt cccggcgcat ggtaagacgc ggcttctgac
     6181 tccagtaagg ccatgcggtc cactttacct tcggcataaa gctggcgaat acgcactttt
     6241 tctttatttg gcaaaccgct tgccatcggt ccagacggca caaacaccgc aggcaaatga
     6301 ccaaacgaca gggctgccat cgtcagaccc gggacaatct tgtcgcacac accgaggaac
     6361 agagcaccat caaacatgtt atgggacagc cccaccgccg cagacatcgc tatcacttcg
     6421 cggcttagca gcgacaattc cattccatcc tgcccctggg tgacaccatc acacatcgcc
     6481 ggaacaccgc ccgcaacctg accaaccgca ttcgcttcat gcagggcttt acgaatgatt
     6541 tctggatagt gttcataagg ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg
     6601 gcgatattgt tacgcaacat gcttttcaaa gaggctttgt cttctggctg gcaggcagcg
     6661 aaaccgtgtg ccaggttacc gcatgccaac tgcgaacgat gaacggtcga cgttttcgct
     6721 tgttctatcc gggcgagata agcagagcga gtctcgcgcg aacgttcaat gattcgattt
     6781 gttacgcgta acaattgtgg attcataaag gctcctgaaa ttgagttgtc agagcaggat
     6841 gattcacaac gcgtttcatt cagaggattt atgagtgaaa cgcctgtaac cggagctcat
     6901 agggcaaaaa cgtttcagtc agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg
     6961 cctgaaagtg taaaaattgt tctacaatct gtgcaagatc atgttaccgg taaaataacc
     7021 ataaaggata agcgcagata ttactcaaac tcattccagg aacgaccatc acgggtaatc
     7081 atcgccaccg aggcaacggg tccccaggtt ccggcctgat acggtttcgg cgcatcattg
     7141 tccatcgccc acgcctcagt aatggagtct acccatttcc aggcttcttc cacttcatca
     7201 cgacgtacaa acagtgcctg aataccacgc atggtttcca gcagcagacg ttcataggca
     7261 tccgccagat gcgtctgatt aaaggtttct gaatagctca gatccagctt ggtgatttgc
     7321 aggttatgtt tgtggtcaag gccaggaact ttattcagta cctggatatc cacgccttca
     7381 tcaggttgca gacggatagt cagtttattc tgcggcagat cctgccacga ctctttaaac
     7441 agattcagtt caggtgtttt gaaatagacc acgacttcag aacatttggt cggcagacgt
     7501 ttaccagtac gcaggtagaa tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg
     7561 atcgccacga aggtttctgt attgctgctc ttgttcgcac cctcttcttc cagatatccc
     7621 ggcacttttt tgccctgggc gaagcccgca gtatattgcc cacgtacggt tttttcgcgt
     7681 acgttggagc ggtcgatgcg gcgcagggac ttcagtactt tcactttttc atcgcggatg
     7741 ctgtctgcgc tcaggtcaga tggcggagac atcgcaatca tgcagagaat ttgtagcagg
     7801 tggttctgaa tcatgtcacg catctgaccg gctttatcaa aatagcccca gcgcccttcg
     7861 atccccactt cttctgccac ggtaatctca acatgatcaa tggtgcgatt gtcccagtta
     7921 ttcacaaaca gggagttagc aaaacgcagc gccaacaggt tcagcaccgt ttctttacca
     7981 agatagtggt cgatacggta aacctggcac tcctcgaagt attcgccaac ctgatcattg
     8041 atttcctgcg aggtcgccag cgacgtcccc agcggtttct ccatgactac gcgtgccggt
     8101 ttagcattca gttttgcctc gccaagccct ttgcaaattg cgccaaaagt gctgggcggc
     8161 atggcaaagt agttaatggt gatacgattt ttttgatcca gcatcgcgcc gagacggttg
     8221 aatgcagcag tgtcattgac atcgagatta caaaaatcca gacgtgcact cagggtgtcc
     8281 cataaacctt catcaatggt ttctttcatg aaagtttcga gcgcctcgcg gacaactttg
     8341 gtatatgccg ctttatccca gtcagcacgc cctacgccga taatccgggt gtccgggttg
     8401 agctgaccgg ctttttccag ttgatacagg gaaggcagca atttacgacg cgcaaggtcg
     8461 cctttcgcgc cgaaaatgac caggtcacag gcctgggctg tttgcgttac cgccatgtca
     8521 ttctccttaa gttaactaac ccggtactta agccagggta tacttgtaat tttcttacgg
     8581 tgcactgtac tgcttttacg agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt
     8641 acgggaaaag cgcgcaaagc gcggcaaaac tgataaaaaa tcatcgtttt ccattcggta
     8701 aaaatctgac actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca
     8761 ttaacacggc acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagtgcgg
     8821 aaatcgtcat tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc
     8881 agctggaaca tttgagcaaa tcagagcgca aagttgccga ggtcattctg gcttcgcccg
     8941 ataacgcgat ccattcgagt attgctgcta tggcactgga agccaatgtt agcgaaccga
     9001 cggtgaatcg tttctgtcgc agcatggaca cgcgcggttt tcctgatttt aaacttcatc
     9061 tggcacagag tctggcgaat ggcactccct atgttaatcg caatgtcaat gaagatgaca
     9121 gcgttgaatc atacacaggg aaaatatttg agtccgcaat ggcaacgctt gatcatgtcc
     9181 gtcattcact ggataaatct gccatcaacc gcgccgtcga cttgctcact caggcaaaaa
     9241 aaatcgcctt tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt
     9301 tctttcgttt taatgttccg gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt
     9361 gtatgaattg tagcgacgga gacgtggtgg tgctgatttc tcacactgga agaacaaaaa
     9421 atctggtcga gctggcgcag ctggctcgcg aaaacgacgc aatggtgatt gccctcacct
     9481 ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag
     9541 atactgacat ttatatgccc atggtttctc gacttgcaca gctgaccgtg atagatgtgc
     9601 tggcgacagg atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca
     9661 aggaagcgct gaaggaatcg cgttttgata agcagttact taatttaagt gacgatcgct
     9721 aaaaacgact gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca
     9781 tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg
     9841 gagtattaca tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca
     9901 acagatcgcg ataataatct tgaaaaagtt atcgcggcgg gtgccaacgt tgtacgtatg
     9961 aacttttctc acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgagatt
    10021 gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaatccgt
    10081 gtatccacct ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac
    10141 gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg
    10201 cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt ccagttaaaa
    10261 gtactggaag ttcagggcat gaaagtgttc accgaagtca ccgtcggtgg tcccctctcc
    10321 aacaataaag gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa
    10381 gacaaagcag acattaagac tgcggcgttg attggcgtag attacctggc tgtctccttc
    10441 ccacgctgtg gcgaagatct gaactatgcc cgtcgcttgg cacgcgatgc aggatgtgat
    10501 gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat
    10561 gacatcatcc tcgcctctga cgtggtaatg gttgcacgtg gcgacctcgg tgtggaaatt
    10621 ggcgacccgg aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagctaaac
    10681 cgagcggtaa tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg
    10741 cgtgcagaag tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctg
    10801 tctgcagaaa ctgccgcagg gcagtatccg tcagaaactg ttgcagccat ggcgcgcgtt
    10861 tgcctgggtg cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag
    10921 ttcgacaatg tggaagaagc tattgccatg tcagcaatgt acgcagctaa ccacctgaaa
    10981 ggcgttacgg cgattattac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt
    11041 atcagctctg gtctaccaat tttcgccatg tcgcgccatg aacgtacgct gaacctgact
    11101 gctctctatc gtggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcagct
    11161 gccagcgaag cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg
    11221 attgtcaccc agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta
    11281 acggtagagt aatctcacct gccccggccg ctgatggccg gggtatttag caacacataa
    11341 attaggacta cttattcaat ccaatcataa tcacagcccc ctgtaaaatg aatacaggga
    11401 tagcctgaat ggagatctaa atagcttgca agacgtcgca tacccgcttc ctctgcaaca
    11461 ctatgtccaa taactgccat cggtaagcca ctatctttgg agtattgtgt ctgcacccat
    11521 gttttaattg aatcgttaat cgtaatataa taatcagcct gatattccat atattgtatt
    11581 ggatcacaaa aacagcctgc cccaatgcca acagttctga ctact
//
LOCUS       DAJVOY010000127        11549 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVOY010000127 DAJVOY010000000
VERSION     DAJVOY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.127"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1074
                     /locus_tag="OQL90_002972"
     CDS             <1..1074
                     /locus_tag="OQL90_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1369675.1"
                     /translation="QIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAV
                     IRYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVP
                     TPFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMA
                     VFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLL
                     SCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKM
                     PPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEE
                     NQGSNLSGPFLPGQSRTFSYRLA"
     gene            1922..3490
                     /gene="cydA"
                     /locus_tag="OQL90_002973"
     CDS             1922..3490
                     /gene="cydA"
                     /locus_tag="OQL90_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1369676.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            3506..4645
                     /gene="cydB"
                     /locus_tag="OQL90_002974"
     CDS             3506..4645
                     /gene="cydB"
                     /locus_tag="OQL90_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1369677.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            4660..4773
                     /gene="cydX"
                     /locus_tag="OQL90_002975"
     CDS             4660..4773
                     /gene="cydX"
                     /locus_tag="OQL90_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1369678.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            4773..5066
                     /gene="ybgE"
                     /locus_tag="OQL90_002976"
     CDS             4773..5066
                     /gene="ybgE"
                     /locus_tag="OQL90_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1369679.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            5216..5620
                     /gene="ybgC"
                     /locus_tag="OQL90_002977"
     CDS             5216..5620
                     /gene="ybgC"
                     /locus_tag="OQL90_002977"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1369680.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            5617..6309
                     /gene="tolQ"
                     /locus_tag="OQL90_002978"
     CDS             5617..6309
                     /gene="tolQ"
                     /locus_tag="OQL90_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1369681.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            6313..6741
                     /gene="tolR"
                     /locus_tag="OQL90_002979"
     CDS             6313..6741
                     /gene="tolR"
                     /locus_tag="OQL90_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1369682.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            6806..7987
                     /gene="tolA"
                     /locus_tag="OQL90_002980"
     CDS             6806..7987
                     /gene="tolA"
                     /locus_tag="OQL90_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1369683.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            8120..9412
                     /gene="tolB"
                     /locus_tag="OQL90_002981"
     CDS             8120..9412
                     /gene="tolB"
                     /locus_tag="OQL90_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1369684.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLVFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            9447..9968
                     /gene="pal"
                     /locus_tag="OQL90_002982"
     CDS             9447..9968
                     /gene="pal"
                     /locus_tag="OQL90_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1369685.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            9978..10769
                     /gene="cpoB"
                     /locus_tag="OQL90_002983"
     CDS             9978..10769
                     /gene="cpoB"
                     /locus_tag="OQL90_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1369686.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            10934..11009
                     /locus_tag="OQL90_002984"
     tRNA            10934..11009
                     /locus_tag="OQL90_002984"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10967..10969,aa:Lys,seq:ttt)
     gene            11045..11120
                     /locus_tag="OQL90_002985"
     tRNA            11045..11120
                     /locus_tag="OQL90_002985"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11078..11080,aa:Val,seq:tac)
     gene            11123..11198
                     /locus_tag="OQL90_002986"
     tRNA            11123..11198
                     /locus_tag="OQL90_002986"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11156..11158,aa:Lys,seq:ttt)
     gene            11250..11325
                     /locus_tag="OQL90_002987"
     tRNA            11250..11325
                     /locus_tag="OQL90_002987"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11283..11285,aa:Val,seq:tac)
     gene            11329..11404
                     /locus_tag="OQL90_002988"
     tRNA            11329..11404
                     /locus_tag="OQL90_002988"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11362..11364,aa:Lys,seq:ttt)
BASE COUNT         2864 a         2713 c         3080 g         2892 t
ORIGIN      
        1 caaattgaag aaagctctga tgatggtgat gaatatgact attcacccgc aaaagaagag
       61 tgggtaatta ccgcagcgaa cgcgaaaccg caatgcgata ttattcatga agcctggcag
      121 agcagggctg ttatccgcta tgacatggca gtgccgctca atttgtcaga acgcagcgcc
      181 cggcaatcca ctggcagagt aggggtggtg ttggttgtca ctcttagtca taacagcagg
      241 cgtattgatg tggatatcaa ccttgataac caggctgacg atcatcgcct tcgtgtcctg
      301 gtccctacac cttttaacac cgacagtgtt ctggcagata cgcagtttgg ttcgctaacg
      361 cgccccgtga acgacagtgc aatgaacaac tggcagcaag aaggctggaa agaagcgccg
      421 gttccggtat ggaatatgct caactatgtt gccttacagg aagggcgtaa cggcatggct
      481 gtctttagcg aagggttacg tgaatttgaa gtcatcggtg aagagaagaa aacctttgcc
      541 attacgttgc tgcgtggcgt gggcttactg ggcaaagaag atctgctttt aaggcctggg
      601 cggccttcgg gaattaaaat gccagtcccg gactcacaac tacgtggtct gctttcttgt
      661 cgcctaagtt tattgagtta taccggtacg ccaaccgccg ctggtgtagc tcagcaggcg
      721 cgagcatggc tgactccagt acagtgttac aacaaaatcc catgggatgc gatgaagctc
      781 aacaaagccg gattcaacgt gccggaaagt tatagtttgt tgaaaatgcc cccagtggga
      841 tgcctgataa gcgcacttaa gaaagctgaa gaccgacaag aagtgatttt acggctgttt
      901 aatccggctg aatcagcaac ctgtgatgcg actgttgctt tcagtcgcga ggtgatttct
      961 tgctcagaaa cgatgatgga tgaacacatt accaccgagg aaaatcaagg ttcaaatcta
     1021 tcggggcctt ttttacccgg ccagtcacgg acgttcagtt accggcttgc ctgaatagca
     1081 atcaaaccga agccacatat gcgcggccag attgttgaca aagggcgctt tgttcatgcc
     1141 ggatacggca tgaacgcttt attcggtcta caaaagcagg caaattcaat atattgcaga
     1201 gatgatgtag acactggcaa gcgtagcgca tcaggtaatt ttgcgtttat cttcactctc
     1261 aagccacgta tatgtggctt tatttttaac aaaataataa ccctgggtga gttaattata
     1321 atataattat aagttaatta aatgttaata tcggcggggg tggatttatg cctttattag
     1381 taatcctgaa actctgcgtc gtattagcca gtgaccaaaa aaagaattaa ggtcaaccgt
     1441 gctgtttttg cttcgtctct ttttatcttt aattgccaac cgaaactaat ttcagcctta
     1501 taactcacac attttaaaca taaatgtcac taaagttacc ttattgaaac atgattaaca
     1561 taatttgtag gaattgatat ttatcaatgt ataagtcttg gaaatgggca tcaaaaagag
     1621 ataaattgtt ctcgatcaaa ttggctgaaa ggcggtaatt tagctataaa ttgatcaccg
     1681 tcgaaaaatg caaatttgct tcaacaaaaa cctgtttatt gtaaggattt tgcggcgtaa
     1741 tatatacgtg ggatcaattt gagtttttat taacatgttt gcaacctttc tttacgccgt
     1801 ttttgtgtgc attcacatgg tatgatgaaa gtgttcaaac aaatttctat tggggcatgc
     1861 gtgtgaccct ttctaacggg gttcactctc ggagtcttca tgcgatgagc aaggagtcat
     1921 gatgttagat atagtcgaac tgtcgcgctt acagtttgcc ttgaccgcga tgtaccactt
     1981 cctttttgtg ccactgacgc tcggtatggc gttcctgctg gccattatgg aaacggtcta
     2041 cgtcctctcc ggcaaacaga tttataaaga tatgaccaag ttctggggca agttgtttgg
     2101 tatcaacttc gctctgggtg tggctaccgg tctgaccatg gagttccagt tcgggactaa
     2161 ctggtcttac tattcccact atgtagggga tatcttcggt gcgccgctgg caatcgaagg
     2221 tctgatggcc ttcttcctcg aatccacctt tgtaggtctg ttcttcttcg gttgggatcg
     2281 tctgggtaaa gttcagcata tgtgtgtcac ctggctggtg gcgctcggtt caaacctgtc
     2341 cgcactgtgg attctggttg cgaacggctg gatgcaaaac ccaatcgcgt ccgatttcaa
     2401 ctttgaaact atgcgtatgg agatggtgag cttctccgag ctggtgctta acccggttgc
     2461 tcaggtgaaa ttcgttcaca ctgtagcgtc tggttatgtg actggcgcga tgttcatcct
     2521 cggtatcagc gcatggtata tgctgaaagg tcgtgacttc gccttcgcta aacgctcctt
     2581 tgctatcgct gccagcttcg gtatggctgc tgttctgtct gttattgttc tgggtgatga
     2641 atccggctac gaaatgggcg acgtgcagaa aaccaaactg gctgctattg aagccgagtg
     2701 ggaaacgcaa cctgcgcctg ctgcctttac tctgttcggc attcctgatc aggaagagga
     2761 gacgaacaaa tttgcgattc agatccctta cgcactgggc atcattgcaa cgcgttccgt
     2821 ggataccccg gttatcggcc tgaaagagct gatggtgcag catgaagaac gcattcgtaa
     2881 cgggatgaag gcgtactctc tgctcgaaca actgcgttct ggttctaccg accaggcggt
     2941 tcgtgaccag ttcaatagca tgaagaaaga cctcggttac ggtctgctgc tgaaacgcta
     3001 tacgccaaac gtggctgatg cgactgaagc gcagattcaa caggcaacta aagactccat
     3061 tccgcgtgta gcgccgctgt acttcgcgtt ccgtattatg gtggcgtgtg gcttcctgct
     3121 gctggcaatc atcgcgctct ctttctggag tgtcatccgc aaccgcattg gcgagaaaaa
     3181 atggctgctg cgcgccgcgc tgtacggtat tccgctgccg tggattgctg tagaagcggg
     3241 ctggttcgtg gctgaatatg gccgccaacc gtgggctatc ggtgaagtgc tgccgacagc
     3301 tgtggcgaat tcgtcactga ccgcaggcga tctcatcttc tcaatggtgc tgatttgcgg
     3361 cctgtatacc ctgttcctgg tggcagaatt gttcttaatg ttcaaatttg cacgcctcgg
     3421 cccaagcagc ctgaaaaccg gtcgctatca ctttgagcag tcttccacga ctactcagcc
     3481 ggcacgctaa gacaggagtc gtcaaatgat cgattatgaa gtattgcgtt ttatctggtg
     3541 gctgctggtt ggcgttctgc tgattggttt tgcagtcact gacggtttcg acatgggggt
     3601 gggcatgctc acccgtttcc tcggtcgtaa cgacaccgag cgtcgaatta tgattaactc
     3661 cattgcacca cactgggacg gtaaccaggt ttggctgatc accgcgggcg gcgcactctt
     3721 tgctgcctgg ccgatggtct atgccgctgc gttctccggc ttctatgtgg cgatgatcct
     3781 cgtgctggcg tctttgttct tccgtccggt cggttttgac taccgctcca agattgaaga
     3841 aacccgctgg cgtaacatgt gggactgggg catcttcatt ggtagcttcg ttccgccgct
     3901 ggtaattggt gttgcgttcg gtaacctgtt gcagggcgta ccgttcaacg ttgatgaata
     3961 tctgcgtctg tactacaccg gtaacttctt ccagttgctt aacccgttcg gcctgctggc
     4021 aggcgtggtg agcgtaggga tgatcattac tcagggcgca acctatctgc aaatgcgtac
     4081 cgtgggcgaa ctgcacctgc gtacccgtgc aacggctcag gtggctgcgc tggtgacact
     4141 ggtctgtttc gcactggctg gcgtatgggt gatgtacggt atcgatggtt atgtcgtgaa
     4201 atcgacaatg gaccattacg cagcctctaa cccactgaat aaagaagtgg ttcgtgaagc
     4261 tggcgcatgg ctggttaact tcaacaacac gccaattctg tgggctattc cggcactggg
     4321 tgtggttctg ccgctgctga ccatcctgac tgcacgtatg gataaagccg cgtgggcgtt
     4381 tgtgttctcc tccctgacgc tggcctgcat catcctgaca gccggtatcg caatgttccc
     4441 gtttgtgatg ccgtccagca ccatgatgaa cgcaagtctg acaatgtggg atgcaacttc
     4501 cagccagctg acgcttaacg tcatgacctg ggttgcggtg gttctggtac cgatcattct
     4561 gctctacacc gcctggtgtt actggaaaat gttcggtcgt atcaccaaag aagatattga
     4621 acgtaacacc cactctctgt actaagtaag gagctaaaaa tgtggtattt cgcatggatt
     4681 ctgggaacgc ttcttgcctg ttcgtttggg gtaatcaccg cgctggcgct tgaacacgtc
     4741 gaatcaggca aagccggtca agaagacatc tgatgagtaa gattatcgcg accttgtatg
     4801 cggtaatgga caagcgcccc ctgcgggcgc tttccttcgt gatggcgctt ctgttagcag
     4861 gatgtatgtt ttgggaccca tctcgttttg ccgcgaagac cagtgaactg gaaatctggc
     4921 atggtttatt gctgatgtgg gccgtctgtg ctggtgtgat tcacggcgtg ggctttcgtc
     4981 cgcagaaggt tctttggcaa gggatttttt gcccattgct tgccgatatt gttctcatta
     5041 tcgggctgat tttcttcttc ttttaaatca gaattctctt taaaaaatta tgggccgctc
     5101 caggcccata aatttttacg ctcccttaac ttgccctcat tcccaaacct caatcgcgcg
     5161 cgtatagtag cagcgtttaa aagctctaac ttttgttgca ttaccgggat gtaaagtgaa
     5221 tacaacgctg tttcgatggc cggctcgcgt ctactatgaa gataccgatg ccggtggtgt
     5281 ggtgtaccac gccagttacg tcgcttttta tgaaagagca cgcacagaga tgctgcgtca
     5341 tcatcacttc agtcagcagg cgctgatggc tgaacgcgtt gcctttgtgg tacgtaaaat
     5401 gacggtggaa tattacgcac ctgcgcggct cgacgatatg ctcgaaatac agactgaaat
     5461 aacatcaatg cgtggcacct ctttggtttt cacgcaacgt attgtcaacg ccgagaatac
     5521 tttgctgaat gaagcagagg ttctggttgt ttgcgttgac ccactcaaaa tgaagcctcg
     5581 tgcgcttccc aagtctattg tcgcggagtt taagcagtga ctgacatgaa tatccttgat
     5641 ttgttcctga aggctagcct tctggttaaa cttatcatgt tgattttgat tggtttttca
     5701 atcgcatcct gggccattat tatccagcgg acccgtattc ttaacgcagc ggcgcgcgaa
     5761 gccgaagcgt ttgaagataa attctggtct ggaatcgaac tctctcgcct ctatcaagag
     5821 agtcagggga aacgggataa tctgactggt tcggaacaaa tattttacag cgggttcaaa
     5881 gagtttgtgc gcctgcatcg tgccaatagc catgcgccgg aagccgtagt ggaaggggcg
     5941 tcgcgtgcta tgcgtatctc catgaaccgt gaacttgaaa atctggaaac gcacattccg
     6001 ttcctcggta cggttggctc catcagcccg tatattggtc tgtttggtac ggtctggggg
     6061 atcatgcatg cctttatcgc cctcggggcg gtaaaacaag caacactgca aatggttgcg
     6121 cccggtatcg cagaagcgtt gattgcgact gcaattggtc tgtttgccgc tatcccggca
     6181 gttatggcct acaaccgcct caaccagcgc gtaaacaaac tggaactgaa ttacgacaac
     6241 tttatggaag agtttaccgc gattctgcac cgccaggcgt ttaccgttag cgagagcaat
     6301 aaggggtaag tcatggccag agcgcgtgga cgaggtcgtc gcgaacttaa gtccgaaatc
     6361 aacattgtac cgttgctgga cgtactgctg gtgctgttgc tgatcttcat ggcgacagcg
     6421 cccatcatca cccagagcgt ggaggtcgat ctgccagacg ctactgaatc acaggcggtg
     6481 agcagtaacg ataatccgcc agtgattgtt gaagtgtctg gtattggtca gtacaccgtg
     6541 gtggttgaga aagatcgcct ggagcgttta ccaccagagc aggtggtggc ggaagtgtcc
     6601 agccgcttca aggccaaccc gaaaacggtc tttctgatcg gtggcgcaaa agatgtgcct
     6661 tacgatgaaa taattaaagc actgaacttg ttacatagtg cgggtgtgaa atcggttggt
     6721 ttaatgacgc agcctatcta aacatctgcg tttcccttgc ttgaaagaga gcgggtaaca
     6781 ggcgaacagt ttttggaaac cgagagtgtc aaaggcaacc gaacaaaacg acaagctcaa
     6841 gcgggcgata attatttcag cagtgctgca tgtcatctta tttgcggcgc tgatctggag
     6901 ttcgttcgat gagaatatag aagcttcagc cggaggcggc ggtggttcgt ccatcgacgc
     6961 tgtcatggtt gattcaggtg cggtagttga gcagtacaaa cgcatgcaaa gccaggaatc
     7021 aagcgcgaag cgttctgatg aacagcgcaa gatgaaggaa cagcaggctg ctgaagaact
     7081 gcgtgagaaa caagcggctg aacaggaacg cctgaagcaa cttgagaaag agcggttagc
     7141 ggctcaggag cagaaaaagc aggctgaaga agccgcaaaa caggccgagt taaagcagaa
     7201 gcaagctgaa gaggcggcag cgaaagcggc ggcagatgct aaagcgaagg ccgaagcaga
     7261 tgctaaagct gcggaagaag cagcgaagaa agcggctgca gacgcaaaga aaaaagcaga
     7321 agcagaagcc gccaaagccg cagcgcagaa aaaagccgag gcagccgctg cggcactgaa
     7381 gaagaaagcg gaagcggcag aagcagctgc agctgaagca agaaagaaag cggcaactga
     7441 agctgctgaa aaagccaaag cagaagctga gaagaaagcg gctgctgaaa aggctgcagc
     7501 tgataagaaa gctgcagccg acaaaaaagc agcagaaaaa gcggctgctg aaaaggcagc
     7561 agctgataag aaagcagcgg cagaaaaagc cgccgcagag gcagatgata ttttcggtga
     7621 gctaagctct ggtaagaatg caccgaaaac ggggggaggg gcgaaaggga acaatgcttc
     7681 gcctgccggg agtggtaata ctaaaaacaa tggcgcatca ggggccgata tcaataacta
     7741 tgccgggcag attaaatctg ctatcgaaag taagttctat gacgcatcgt cctatgcagg
     7801 caaaacctgt acgctgcgca taaaactggc acccgatggt atgttactgg atatcaaacc
     7861 tgaaggtggc gatcccgcac tttgtcaggc tgcgttggca gcagctaaac ttgcgaagat
     7921 cccgaaacca ccaagccagg cagtatatga agtgttcaaa aacgcgccat tggacttcaa
     7981 accgtaatcg cgatgttgac tgttcggacg gtcaacatca ggcaccggtt gccacggggt
     8041 tctggtagtt ttgtgtattt tagtttgtta acattctgct aaattatcgt gggccatcgg
     8101 tccagataag ggagatatga tgaagcaggc attacgagta gcatttggtt ttctcatact
     8161 gtgggcatca gttctgcatg ctgaagtccg cattgtgatc gacagcggtg tagattccgg
     8221 tcgtcctatt ggtgttgttc ctttccagtg ggcggggcct ggtgcggcac ctgaagatat
     8281 tggcggcatc gttgctgctg acttgcgtaa cagcggtaaa tttaatccgt tagatcgcgc
     8341 tcgtctgcca cagcagccgg gtagtgcgca ggaagtacaa ccatctgcat ggtccgcact
     8401 gggcattgac gctgtagttg tcggtcaggt cactccgaat ccggatggtt cttacaatgt
     8461 tgcttatcaa cttgttgaca ctggcggcgc accgggtact gtacttgctc agaactcgta
     8521 caaagtgaac aagcagtggc tgcgttatgc tggtcatacc gccagtgatg aagtgtttga
     8581 aaaactgacc ggcattaaag gtgcgttccg tacccgtatt gcctacgttg ttcagaccaa
     8641 cggcggtcag ttcccgtatg aactgcgcgt atctgactat gacggttaca accagtttgt
     8701 cgttcaccgt tcaccgcagc cgctgatgtc tccggcgtgg tcaccagacg gttctaaact
     8761 ggcttatgtg accttcgaaa gcggtcgttc cgcgctggtt attcagacgc tggcaaatgg
     8821 cgctgtacgt caggtggctt cattcccgcg tcacaacggt gcacctgcat tctcgccaga
     8881 cggcagcaaa ctggcattcg ccttgtcgaa aaccggtagt ctgaacctgt acgtaatgga
     8941 tttggcttct ggtcagatcc gccaggtgac tgatggtcgc agtaacaata ccgaaccgac
     9001 ctggttcccg gatagccaga acctggtatt tacttctgac caggccggtc gtccgcaggt
     9061 ttataaagtg aatatcaacg gcggtgcgcc acaacgtatt acctgggaag gttcgcagaa
     9121 ccaggatgcg gatgtcagca gcgacggtaa atttatggta atggtcagct ccaatggtgg
     9181 gcagcagcac attgccaaac aagatctggc aacgggaggc gtacaagttc tgtcgtccac
     9241 gttcctggat gaaacgccaa gtctggcacc taatggcact atggtaatct acagctcttc
     9301 tcaggggatg ggatccgtgc tgaatttggt ttctacagat gggcgtttca aagcgcgtct
     9361 tccggcaact gatggacagg tcaaattccc tgcctggtcg ccgtatctgt gataataatt
     9421 aattgaatag taaaggaatc attgaaatgc aactgaacaa agtgctgaaa gggctgatga
     9481 ttgctctgcc tgttatggca attgcggcat gttcttccaa caagaacgcc agcaatgacg
     9541 gcagcgaagg catgctgggt gccggcactg gtatggatgc gaacggcggc aacggcaaca
     9601 tgtcttccga agagcaggct cgtctgcaaa tgcaacagct gcagcagaac aacatcgttt
     9661 acttcgatct ggacaagtac gatatccgtt ctgacttcgc tcaaatgctg gatgcacatg
     9721 caaacttcct gcgtagcaac ccgtcttaca aagtcaccgt agaaggtcac gcggacgaac
     9781 gtggtactcc ggaatacaac atctccctgg gtgaacgtcg tgcgaacgcc gttaagatgt
     9841 acctgcaggg taaaggcgtt tctgcagacc agatctccat cgtttcttac ggtaaagaaa
     9901 aacctgcagt actgggtcat gacgaagcgg catactccaa aaaccgtcgt gcggtactgg
     9961 tttactaaga gaattgcatg agcagtaact tcagacatca actattgagt ctgtcgttac
    10021 tggttggtat agcggccccc tgggccgctt ttgctcaggc accaatcagt agtgtcggct
    10081 caggctcggt cgaagaccgc gtcactcaac ttgagcgtat ttctaatgct cacagccagc
    10141 ttttaaccca actccagcaa caactttctg ataatcaatc cgatattgat tccctgcgtg
    10201 gtcagattca ggaaaatcag tatcaactga atcaggtcgt ggagcggcaa aagcagatcc
    10261 tgttgcagat cgacagcctc agcagcggtg gtgcagcggc gcaatcaacc agcagcgatc
    10321 aaagcggtgc ggcggcatca acgacgccga cagctgatgc tggtactgcg aatgctggcg
    10381 cgccggtgaa aagcggcgat gcaaacacgg attacaatgc agctattgcg ctggtgcagg
    10441 ataaatcccg ccaggatgac gcaatggtgg catttcagaa tttcatcaaa aattaccctg
    10501 attcaactta cctgccaaac gccaattatt ggctgggtca gttaaactac aacaagggta
    10561 aaaaagatga tgcggcgtac tattttgctt cggtagtgaa aaactatccg aagtcaccaa
    10621 aggctgctga tgcgatgttt aaagtcggcg tcatcatgca ggacaaaggt gacaccgcaa
    10681 aagcgaaagc cgtataccag caggttatca gtaaataccc tggtactgat ggcgctaaac
    10741 aggcacaaaa acgtctgaac gcgatgtaat gcataacaca cgaccagaag ttgcattatt
    10801 tctggtcgtg tcgtgcgaat cataagcagt tgagtgatct gcatcgaaat ttttgttgcg
    10861 ctcaagtctg aaatcagtaa tatatgccgc cgttgccacg ggatatcaaa caaaccgaaa
    10921 gcaacgaaaa agtgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt
    10981 cgcaggttcg aatcctgcac gacccaccac taacatagtt agttgtagta tccagcgtag
    11041 tattgggtga ttggctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    11101 gatcccgtca tcacccacca ccgggtcgtt agctcagtcg gtagagcagt tgacttttaa
    11161 tcaattggtc gcaggttcga atcctgcacg acccaccact aattacgatg ggttcggtgg
    11221 aagtagtttg tagtatccag cgcagtatcg ggtgattagc tcagctggga gagcacctcc
    11281 cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccactcgg gtcgttagct
    11341 cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc
    11401 accaatttta acatcaaact cagatgttaa gcgtgaagga taacgttgcg ccagcaacgg
    11461 cccgtagggc aagcgaagcg agtcatcctg cacgacacac caatcttaaa gattggcccc
    11521 gagtagaaat ctttcaggta acacccgta
//
LOCUS       DAJVOY010000128        11402 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVOY010000128 DAJVOY010000000
VERSION     DAJVOY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.128"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..743)
                     /gene="mak"
                     /locus_tag="OQL90_002989"
     CDS             complement(<1..743)
                     /gene="mak"
                     /locus_tag="OQL90_002989"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1369687.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDV"
     gene            868..1779
                     /gene="rdgC"
                     /locus_tag="OQL90_002990"
     CDS             868..1779
                     /gene="rdgC"
                     /locus_tag="OQL90_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1369688.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(1857..1940)
                     /locus_tag="OQL90_002991"
     CDS             complement(1857..1940)
                     /locus_tag="OQL90_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369689.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(1937..2278)
                     /locus_tag="OQL90_002992"
                     /pseudo
     CDS             complement(1937..2278)
                     /locus_tag="OQL90_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(2426..2710)
                     /gene="ppnP"
                     /locus_tag="OQL90_002993"
     CDS             complement(2426..2710)
                     /gene="ppnP"
                     /locus_tag="OQL90_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1369690.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(2782..3459)
                     /locus_tag="OQL90_002994"
     CDS             complement(2782..3459)
                     /locus_tag="OQL90_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1369691.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(3717..3908)
                     /gene="yaiA"
                     /locus_tag="OQL90_002995"
     CDS             complement(3717..3908)
                     /gene="yaiA"
                     /locus_tag="OQL90_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1369692.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(3958..4482)
                     /gene="aroL"
                     /locus_tag="OQL90_002996"
     CDS             complement(3958..4482)
                     /gene="aroL"
                     /locus_tag="OQL90_002996"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1369693.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(4665..5123)
                     /locus_tag="OQL90_002997"
     CDS             complement(4665..5123)
                     /locus_tag="OQL90_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1369694.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5243..6052
                     /gene="proC"
                     /locus_tag="OQL90_002998"
     CDS             5243..6052
                     /gene="proC"
                     /locus_tag="OQL90_002998"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1369695.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(6069..7184)
                     /gene="adrA"
                     /locus_tag="OQL90_002999"
                     /gene_synonym="dgcC"
     CDS             complement(6069..7184)
                     /gene="adrA"
                     /locus_tag="OQL90_002999"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1369696.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(7286..7606)
                     /gene="psiF"
                     /locus_tag="OQL90_003000"
     CDS             complement(7286..7606)
                     /gene="psiF"
                     /locus_tag="OQL90_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1369697.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(7725..9140)
                     /gene="phoA"
                     /locus_tag="OQL90_003001"
     CDS             complement(7725..9140)
                     /gene="phoA"
                     /locus_tag="OQL90_003001"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1369698.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(9241..9501)
                     /gene="iraP"
                     /locus_tag="OQL90_003002"
     CDS             complement(9241..9501)
                     /gene="iraP"
                     /locus_tag="OQL90_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1369699.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(9728..9885)
                     /locus_tag="OQL90_003003"
                     /pseudo
     CDS             complement(9728..9885)
                     /locus_tag="OQL90_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9963..11057
                     /gene="ddlA"
                     /locus_tag="OQL90_003004"
     CDS             9963..11057
                     /gene="ddlA"
                     /locus_tag="OQL90_003004"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1369700.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(11081..11293)
                     /locus_tag="OQL90_003005"
     CDS             complement(11081..11293)
                     /locus_tag="OQL90_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1369701.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGAVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
BASE COUNT         2842 a         2954 c         2723 g         2883 t
ORIGIN      
        1 atcacatccg gatcgagaat attcacgaca tgtgccagcg attttgccag ccgcagctcg
       61 tagcgacgca atgccagttc cgctaccgga tcgctttctt caaccaggcg gataatttca
      121 ctgcctttca gcgcatgtcc gctcaaacga cgataatcca tcgcgaatcc cgtgcccgaa
      181 ataaaggttt caatacaacc ttgtttaccg caataacaag ggacttcctc gcgataacgc
      241 agttcgtctt cgtccatcca cggtagcgga ttgtgtcccc actcacctgc cgtgccattg
      301 ccgctgatat gcgcccgccc attgaatgcc acgcccgcgc cgcatcccgt gccgataatc
      361 acggcaaata ccgtctgcgc tcccgctgcc gcgccatcta ctgcttctga aaccgccaga
      421 cagttagcgt catttgccag ccgcacttcc cgctgcaacc tcgcgcttaa gtctttatcg
      481 aatggctgac cgttgagcca ggttgaattg gcattcttca ccacaccggt gtaaggcgaa
      541 attgagccag gaatgcccat acctaccgtt ccgcgctgcc ccgtcgcctg ctccgccata
      601 tcaaccaacg tggcgatcgt ttcaatagtc tgccggtaat catcacgcgg cgtgggcaga
      661 cgatggcggt acaactgctc ccctgcatcg cccagtgcaa tcacttcagt tttggtgccg
      721 cctaaatcga tacctatacg cacggtactc tccttatttt tttcaatatc aatagcgtag
      781 agacggacaa ccggattggc aatgcaaggc cgccgacaat tcgttatcat gcccgctaaa
      841 tttaacaaca aggccgtgga aattatcatg ctgtggttca aaaatttaat ggtttaccgt
      901 cttagccgcg agatttcgct gcgtgcagaa gagatggaaa aacagctagc ctcgatggca
      961 tttaccccat gcggcagcca ggacatggcg aagatgggct gggttcctcc gatgggatcg
     1021 cacagcgatg cgttaacgca cgttgccaat ggtcaaattg ttatctgcgc gcgcaaagaa
     1081 gaaaaaatcc tcccgtctcc ggtgattaag caggcgctgg aagcgaaaat cgccaaactg
     1141 gaagcggaac aggcgcgtaa gctgaagaaa accgaaaaag attcgctgaa agacgaagtg
     1201 ctgcactctc tgttgccgcg tgctttcagc cgttttagcc agacaatgat gtggatcgac
     1261 acggttaacg gtttgattat ggtggactgc gccagtgcca aaaaagcgga agatacgctg
     1321 gcactgctgc gtaaaagcct ggggtcgtta cctgtggtac cattgagcat ggaaaacccg
     1381 attgaactga cgctgaccga atgggttcgc tccggtagcg cggcacaggg cttccagctg
     1441 cttgatgaag ccgagctgaa atcgttgctg gaagatggcg gcgtgatccg cgcgaagaaa
     1501 caagatctga ccagcgaaga gatcaccaat cacatcgaag ccggaaaagt ggtgactaaa
     1561 ctggcgctcg actggcagca gcgcattcag tttgtgatgt gcgacgatgg ttcgctcaag
     1621 cgtctgaagt tttgcgacga gctgcgcgat caaaacgaag atatcgaccg tgaagatttc
     1681 gcccagcgtt ttgatgccga tttcatcctg atgactggtg aactggcagc gttaattcaa
     1741 aacctgattg aaggattagg tggcgaagca caacgttaat tgctgatttt cctttaatgc
     1801 cggatgcgac gcctgccgcg tcttatccgg cgtacgaagc cacaccaggg atataattat
     1861 tcgctacggc ggacaataat ttttagcgca gcaatattat gcgttttatg ctgtaacttg
     1921 ctccatggac gttgtgtcat tgtttttcct caagccagtg acgcgcttca ctgcgaattt
     1981 tccgcattgc cccggttttc actaaatccc gtaacagcga aagatcgggt tgttcgacaa
     2041 ataagagtaa tgatggatcc tcttttaacc acgtcgtttt cacatcggca gcgagctgtg
     2101 gattattcat agctagcgac cgatgccgag actcggtcaa tgtcgttaac aacgacgccg
     2161 gggtatgtgc gttgcgtaat gccgtttggc gcaaatgatc attgcgactt tgcagcaatg
     2221 cacgccacca ttcatcatct gatttatctt ttatcgtagc cagttccgca acaatatctg
     2281 gagatcttca ctgatattta tatcaacctt gatgaattat gaatatatcg gaaattgaaa
     2341 aaatcgcagc aaaggaaata ttattatggg ttaaagagag gatatttcag aaaaatgccc
     2401 tcccgttcaa ggggaaggcg aggaattaca gatagcggca cagataagag gtgggttcgg
     2461 caacttgcag atgaaactca ctgtgaccgg gaacattaaa caccgaaccg gcttcataca
     2521 cctgccagtc ggtcgcatca ggcagtaaca cattcagcgc gccactgatt accgtcatct
     2581 cttccggctc agcggtgctg aaggtgtatt cgccttcaac cataacaccc acgctggcgc
     2641 gaccagtgct gctgctggaa aagccgattg atttcacttt gccggaaaag tactcattac
     2701 tttgaagcat aaactggccc tgtctggtag ttgaacatct acttcactat aggggccaga
     2761 ggcgctggct gtcacgcaaa attacaccag taattccgca gccagccgtg caatcaatac
     2821 gttagacagc aagacaggaa catcgagctg tttttgcagt aaatcgcgat gacgttggtg
     2881 aaatcctaaa caatccagca tgatgacatc agcccctttt gccagtaatt ctttccctgc
     2941 atcaatgatt ttttgttctg aatcatgaat ggggttaccc aatgaaaata ccggcgattt
     3001 ctgcaaaatt tgccattttt gcgcctgaac gggcagcatc tcctcaaccg gaacgataac
     3061 ccccacctga tgatcttcaa caatagagga aaccagtgga ggcaatattc gcgacggctc
     3121 aagaaagatc gtattacgcg cagtcatact actaatgttt gctgtactca ttaataaaat
     3181 gacgtcataa ccctgattat cgagcacttc aaccacacct tgcaggtctc gctccacttt
     3241 acgacgcgaa acatgggcca gctggttgtc atttaataat gtgagaatgg tgtcttcgcc
     3301 tgcttctggc gcgtactccg ccatcacttc ttcacgactt aacttcccca gcaggctatg
     3361 atgggaaata ttatcttcgt caatgtattc cgtcaggagc ggcaaaacct cctgcatagg
     3421 tacaatgccg atggtcagga tcgccaacga cgcactcatt gttcatccac cttacttttc
     3481 tttcgtcgtt aattaccggg caagtgtgaa gcaccactgc tgacacttac ttgtgctaca
     3541 aatgacaaaa agcgtagcag cagacgtgca cggcataatg taaagattcc aaatgattcc
     3601 agtaatggat tgttattgtt taatattcta attattagaa aaacatgaat tatgaaaaat
     3661 gtgacgcaga tcccttgtag tggcactcag aatcccttcc gagcagtctg atgcggtcac
     3721 tctttgtcag ggtcctcata gcgttttttc gcatccatcg cttcctgagc ggtcggatgt
     3781 tcactgatca acgagtctgg tttaggatga tcggctctga gttggtacca ggttaccgtc
     3841 tgtcctggct ttcctttttc aatcgtcact atatatgctt cacgaggata aggcggtttc
     3901 gttggcatcg ttcttcctta tttcacggaa tgaacgttaa gtataggcgc tctaaaatca
     3961 acaattgatc gtctgtgcca gggcgctacg aatttcagaa atcacctggc tgggttcgtt
     4021 tgttgcgtcg atgataatat gcgcaacttc gcgatatagc gcatcgcgtt cttccagcac
     4081 ttcctgaact tcttcgctca gcggttttcc cgttaaggtt ggccgtaaat cttcttccgg
     4141 tgcagcttgc agtcggttaa ccaggactga tactggcgca cacaaataaa ccacgatccc
     4201 gttattttgc atgaagtgac gattaaattc cgtcagaata atgccgccgc ctgtagcgat
     4261 aacggtggat ggcgcagtta ccgcttccag cgccgccgtt tctctggcgc gaaatcccgc
     4321 ccactcttcc ctttcgacga tctccgcgac cgtcatattg agctgtgatt gcaaccactg
     4381 atcggtatcg acaaaccgac ggttaagcga atcggcaagg gccattccga ccgttgtttt
     4441 accacagccc cgaggcccga tcagaaaaag aggttgtgtc atcgtgggtt ttccccaata
     4501 ggtcgcaatg cggcgaaagc cggtgtcatg agaatagcga tcattccatc aaactagtac
     4561 aatttcgatt gtaaagaaaa aattccactt aaagtgaaaa tctcaataca tcccttacta
     4621 taccaataaa tattcaagaa tgaagtgtaa ataataaatt acatttagcc acgactacgt
     4681 tgcacttcca gccaccactt ctccagctcc gcggcaaagg cctggcggtc acgttgtgaa
     4741 aggctatctg gtccgccggt ctggatccca ctggcacgta aggtatccat aaaatcgcgc
     4801 atcgtcaggc gctcacgaat ggtcgctggc gtgtaacgtt cgccgcgcgg attaagcgcc
     4861 gctgcgcctt tctcgatggc ttcagcagcc aaaggtatat ctgcggtgat caccaaatcg
     4921 cccgcttcac actgccggac aatttcgtta tcggcaacgt cgaaacctgc cgcgacgcgc
     4981 agcgtacgaa taaatcgcga tggcggcacg cgtaaactct ggtttgctac cagtaccagc
     5041 ggcatctgca tacgttccgc cgcgcgatac aaaatctctt taattacatt gggacacgcg
     5101 tcagcatcca cccaaattgt cataaagtca tcctttgttg ggtaatcctc tattgtgtcg
     5161 cgcttttgcc ttccggcata gttctgttta tgcttctgcc agcgattatc aaaacaatga
     5221 atttcacggc aggagtgagg caatggaaaa gaaaatcggt tttattggct gcggcaatat
     5281 gggaaaagcc attctcggcg gtctgattgc cagcggtcag gtgcttccag ggcaaatctg
     5341 ggtatacacc ccctccccga ataaagtcgc cgccctgcat gaccagttcg gcatcaacgc
     5401 cgcagaatcg gcgcaagaag tggcgcaaat cgccgacatc atttttgctg ccgttaaacc
     5461 tggcatcatg attaaagtgc ttagcgaaat cacctccagc ctgaataaag actctctggt
     5521 cgtttctatt gctgcaggtg tcacgctcga ccagcttgcc cgcgcgctgg gccatgaccg
     5581 gaaaattatc cgcgccatgc cgaacactcc cgcgctggtt aatgccggga tgacctccgt
     5641 aacgccaaac gcgctggtaa ccccagaaga taccgctgat gtgctgaata ttttccgctg
     5701 ctttggcgaa gcggaagtaa ttgctgagcc gatgatccac ccggtagtcg gtgtgagcgg
     5761 ttcttcgcca gcctacgtat ttatgtttat cgaagcgatg gccgacgccg ccgtgctggg
     5821 cgggatgcca cgcgcccagg cgtataaatt tgccgctcag gcggtaatgg gttccgcaaa
     5881 aatggtgctg gaaacgggag aacatccggg ggcactgaaa gatatggtct gctcaccggg
     5941 aggcaccacc attgaagcgg tacgcgtact ggaagagaaa ggcttccgtg ccgcagtgat
     6001 cgaagcgatg acgaagtgta tggaaaaatc agaaaaactc agcaaatcct gatgactttc
     6061 gcctgacgtc aggccgccac ttcggtgcgg ttacgtccgg ctttctttgc tttgtaaagc
     6121 gccaaatctg ccgatttcaa ccactcacga tagtgactca tttgtgggtt cagcggcgca
     6181 acccccacac taatccgtaa agttacctgt ggcgtattcg gcaagcgtaa cgtgtttagc
     6241 ccttcacgca cccgtaacat ggcggtaatg gcgctctcag ctggcgtacc ggacatgatt
     6301 actgcaaact catcgccgcc aaaccgacca atcacatcgc taccgcgcag ggtaatttgt
     6361 aactgtcggg taagcgccac aatcgcttca tcgcccacat catgacccca ggtatcgttg
     6421 atgctcttga aatggtcgat atcgataatc agtaacgttg catcgcgatt atgccgccga
     6481 cagttatcaa attcattgcg taacatagtt tcccaatgac gtcggttata cacgcccgtc
     6541 atgccgtcgc gggtactcat gacctgcaac ctgcgtttat gttccgccag tttggttgcc
     6601 gtctggtagc tgacccagcc aaacagcaga ggataaacga caataatggg aagggagagc
     6661 caccattcca gcggcgcact attgaacgac acggtaatgc ccgtaagctc gagggtgaca
     6721 aggcaggaaa ccaccatcaa caccagaccc gcgacaaaca gacgggggcc gcctgccccc
     6781 atcaaattca gacacataat catcaacata gcggtggaag gcagcacgtt tacgcccatt
     6841 acgcctaccc acattcccgc taatactgca tcggttttta agttgtaaat ttcccggcta
     6901 agcggatcga cggccctgct cgctatctgc caggctaaat gcggccagac gaacgcccag
     6961 ccgaccaaca ctagccacca ccagcccggc ggcgggtgcg aaaccagcgt tgaagcaatc
     7021 ggtaagaaca tgccagccag gccaaccgca cggggtagtc tgacgcgacg ggcgaagcgc
     7081 agcccggacc gctgatgttc gttttgagga gttaaaggag gttcctcccc atgcgccgcc
     7141 gcttttttga aaaagttttc atcattcatt atttttggga acattcagaa acaattttcc
     7201 caaattatag agacggggct aaaggcagaa gtatgaaatt tcgggtgagc cactttcgta
     7261 gctcacccgt tagcactatc aggtatcagg cggctttctt gaggcaggca ctcataaact
     7321 tattacgatc atcacctttc agagattgtt gcgttgcttg attattacat tcgcgcatct
     7381 tttgctgctg tggcgtcaaa cttttttcac caggcgcaga cttgctgttc ttcaggcaat
     7441 cactcatgta ggtcttacga gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac
     7501 aggaggtcat acgctgttgt tgtggggtta acgttctctc ggcagcgccg actgtggtta
     7561 aaaaaaccag accgaaaagc aaggtaacca gtaatgtaat tttcatagca ccatccctct
     7621 tcatgtttta accatgagcg tatgcgcccg agatttgcca ttaagtctgg ttgctaacag
     7681 caaaaaaacc acccggcagc gaaaattcac tgccgggcgc ggttttattt cagccccagg
     7741 gcggctttca tggtgtagaa gagatcggtc tggtcggtca gtccaacgac attggcggca
     7801 tgcgggccat acgccgcaat acgcaactga ctgccggtat gttcttgtga atcctcttcg
     7861 gagttcccgt agctcatcac catcactgcg ccatctttgg tatttagcgc ctgggtgagg
     7921 cccggagctt tggtatccgg cgcgacaatc tggctggcgt gggcgtgatc agcggtgact
     7981 atgaccagcg tgttgccatc ctttttagcg aattccagcg cccgttgtac ggcttcatcg
     8041 agatcgaccg tctcgccaat ttgcccacaa ggattcgcag cgtgatcctg tttatcgatt
     8101 gacgcacctt caacttgcag gaaaaatcct ttctcatttt tactcaacaa ttcaatggct
     8161 ttgtcggtca tctgcgccag ggttggtaca ctgtcattac gttgcggatt aggcgtacag
     8221 gtgactgcgg gcttatcgat attgccgtgg tacgttgctt tcggtcctag ccagcgcact
     8281 ggcatattgc cgtcagcaaa cagtccaagc agaggttttt gctgattcgc ttccgtcacc
     8341 gcatttagcg aggcggcatc gctgaccaac tgataaccac gcgcctgtgc ctgttcacgc
     8401 agcgtttttc cctgccattc accggcggtt gccgtttcag caaaggtttt tgcgccaccg
     8461 ccaagcgtaa cgtcggcacg agcattaagc agctgttcgg taatcgatcc ttttccgcct
     8521 ttttccagag cgttacccgg acatttttca ctggtcgcgc tcggaccgta gcatttgcgc
     8581 gaagtcacat gtgccaccag cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg
     8641 ttaccggtcg ccagacctgc ggcttttgcc atttccagaa tcgttgggtg atctttttcg
     8701 tgaatatcga cgcccagcgc gccgttatag gttttgacac cggttgacca ggcggttgct
     8761 gatgcagccg aatcggtgac gtagtccggt ttgccggttt ttttattcag cgcatagtga
     8821 gtgtattgcc cggtaagcgg taaggcatcg atacctttaa aaaagccgcc cgcaccttcg
     8881 gcataattac gtgcggctgt aatttccgaa tcccccatcc catcgccaat cagcaaaata
     8941 atattttttg caggtttatc gctaagagaa tcacgcagag cggcagtctg atcacccgtt
     9001 aaacggcgag caccgccggg tgcagtaata tcgccctgag cagcccggtt ttccagaaca
     9061 ggcatttctg gtgtccgggc ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt
     9121 gcaatagtgc tttgtttcac tttattttct ccatgtacaa atacattaaa aaataaaaac
     9181 aaagcgacta taagtctcgg ccgtgacaac tttatgacag ctgttgaaaa gattaacttt
     9241 ttactgacga ggatgcttca ataacttctt tacataattg cgcagcagct ccgtatcgtc
     9301 gtcaggaatg ctggcatcag gctttacctc gtaaagagcc ccctccacct gttcaatcaa
     9361 gcgctgttgg tcattttgtg ccatattgcg aagcattgca gtgacgataa tctccaaagc
     9421 ttctacctgc gcgcacagtt ctttcgactc ttcttctttt tgggcaagct ttaataacaa
     9481 ctcagcaatg agatttttca tgtctgtatt tccttatcca acgtatggag aagttaacat
     9541 tatgtttcac aacttcatag tgtctggaag tattattttt atgacaaagg aaatactttg
     9601 cgcaaatgtt ctttgtttga ttaccagcga aacgtttctc atatttaaat aattaaccac
     9661 acaaagaaca ggcctttaat tatcaacctt tattataaac aaaagttatg aaatatattc
     9721 tctgcgttta cttacaacta agagaaaaaa ctcaccgcat ggccatttaa gccaggaata
     9781 aattagcttc ccgttcatca tttttgtgat ctcgcgcaca tctttgtaat aacgcacttt
     9841 cttcaccatt acgttgcacg ctttgtcagc agaagattgc aacattctgt cagaaggccg
     9901 cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg ttagacggga taggttttta
     9961 agatggaaaa actgcgggta ggaatcgttt ttggtggtaa atcagcggaa catgaagtgt
    10021 ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa aagtcgcttc gacgttgtgc
    10081 tgctgggcat tgataaacaa gggcaatggc acgtcagcga tgccagcaat tatctgctaa
    10141 atgcagacga tcctgcccat attgcgttgc gcccttcggc gactagcctt gcgcaggtgc
    10201 caagtaaaca tgagcatcag cttatcgacg cgcaaaacgg tcagccgtta ccgacggtgg
    10261 atgtcatttt cccgattgtc cacggtacgc tgggcgaaga tggttccttg cagggaatgc
    10321 tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct ggcttcagca gcctgtatgg
    10381 ataaagatgt taccaaacgt ctgctgcgcg atgccgggct gaacattgcg ccatttatta
    10441 ccctgacgcg cgccaatcgt cacaacatta gttttgccga agtggagtct aaactgaggt
    10501 taccactgtt tgtaaaaccg gctaatcagg gctcttctgt tggtgtcagc aaagtaacca
    10561 gtgaagaaca gtacgcaatt gccgtcgatc tggcgttcga attcgatcat aaagtgatcg
    10621 ttgagcaagg gatcaaaggt cgtgagatcg aatgcgcagt tctgggcaac gacaacccgc
    10681 aagccagcac ctgtggcgag atcgtactca ccagcgactt ctatgcctac gacaccaagt
    10741 acattgacga agatggcgcg aaagtggtag ttccggcagc cattgcgcca gaaatcaacg
    10801 ataagatccg ggcgattgcc gttcaggctt atcaaacgtt gggatgcgca ggcatggcgc
    10861 gtgtagacgt gtttttaacc ccagagaacg aagtggtgat caacgagatc aacacactgc
    10921 ctggcttcac caacatcagt atgtatccga agctgtggca agccagcggt ctgggttaca
    10981 ccgatctgat cacacgtttg attgagctgg cgctggagcg tcacgctgcg gataacgcat
    11041 tgaaaaccac aatgtaatat tttaacgccc gataatattc ttattgggcg ttttcagttt
    11101 cttcatcgcg acgacgaata atcaaccccg ccagccagaa actaatcacc cacgtcacca
    11161 gaccgacggc ataggtctgc caaccttttg cttcaaatcc cagtaacccc accgcgccat
    11221 taagaatgaa tataaggcca atggcgaacg catagtagtg ccagtcacgg cggattttta
    11281 caggcagatt catggcagct ccgttaacga aaaacaacat ccttgcatat tctgtaacaa
    11341 ccgtctgtgg tgtagacaac attttcgtaa ttgttaataa atttaactta aggttgccat
    11401 tt
//
LOCUS       DAJVOY010000129        11385 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVOY010000129 DAJVOY010000000
VERSION     DAJVOY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.129"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..911
                     /gene="gap"
                     /locus_tag="OQL90_003006"
     CDS             <1..911
                     /gene="gap"
                     /locus_tag="OQL90_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1369702.1"
                     /translation="INDLTSPKILAYLLKHDSNYGPFPWSVDFTEDSLIVDGKSIAVY
                     AEKEAKNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTIVYNV
                     NDDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQSLVDGPRG
                     KDLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVTELVSILGK
                     KATAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGDLQLV
                     KTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(953..2393)
                     /gene="aldA"
                     /locus_tag="OQL90_003007"
                     /pseudo
     CDS             complement(953..2393)
                     /gene="aldA"
                     /locus_tag="OQL90_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2548..3390)
                     /locus_tag="OQL90_003008"
     CDS             complement(2548..3390)
                     /locus_tag="OQL90_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS1369703.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3662..7564)
                     /gene="hrpA"
                     /locus_tag="OQL90_003009"
     CDS             complement(3662..7564)
                     /gene="hrpA"
                     /locus_tag="OQL90_003009"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1369704.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7765..8370
                     /gene="azoR"
                     /locus_tag="OQL90_003010"
     CDS             7765..8370
                     /gene="azoR"
                     /locus_tag="OQL90_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1369705.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8421..9737)
                     /locus_tag="OQL90_003011"
     CDS             complement(8421..9737)
                     /locus_tag="OQL90_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCS1369706.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9727..11385)
                     /locus_tag="OQL90_003012"
                     /pseudo
     CDS             complement(9727..11385)
                     /locus_tag="OQL90_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         2840 a         3090 c         2709 g         2746 t
ORIGIN      
        1 ctattaatga tctcacttcc ccaaaaattc tcgcctacct gctgaaacat gattcaaact
       61 acggaccatt cccctggagc gttgatttta cggaagattc acttatcgtt gatgggaaaa
      121 gtatcgcggt ttacgccgaa aaagaggcta aaaatattcc gtggaaagcg aaaggtgcag
      181 aaatcattgt cgaatgtact ggcttttata cctccgccga gaaatcgcag gcgcatcttg
      241 atgctggtgc gaagaaggtg ttgatttccg cccctgccgg tgaaatgaaa actatcgttt
      301 ataacgtcaa tgacgacact ctggatggca acgacaccat tgtttccgtg gcgtcatgca
      361 ccactaactg tcttgcgccg atggccaaag ccttgcatga cagtttcggg atagaagtcg
      421 gcacgatgac gaccattcat gcctatactg gcacccagtc actggtggat ggcccacgtg
      481 gtaaagattt acgtgcttca cgcgcagcgg cagaaaatat cattccccac actacggggg
      541 cggcaaaagc cattggtctg gagatcccgg aactgagcgg caaactgaaa ggtcatgcgc
      601 aacgcgtgcc ggtgaaaaca ggttcggtca ctgaactggt atcgattctc ggaaaaaaag
      661 cgactgccga agaggtgaat aacgcactta aacaagcaac caccaataac gagtcatttg
      721 gttataccga tgaagaaata gtctcttccg atatcattgg cagccatttc ggttcggtgt
      781 ttgatgccac gcaaacggaa attaccgccg tgggcgattt acaactggtg aaaacggtcg
      841 cctggtacga taacgaatat ggcttcgtca cgcagcttat tcgcaccctc gaaaaattcg
      901 ctaaactctg acgcgcacag gcggaggaaa aaacctccgc ctcttttact cattacgact
      961 gtaaataaac cacctgggtc tgcagatatt catgcaggcc atgtttacca tctgcgccgc
     1021 caataccgga tttacgccat ccggcgtgga agccttgcat agcttcgaac ttttcacggt
     1081 tgatgtaagt ttcaccaaac ttcagccctt taatggcttt catcgcgacg ttcagatttt
     1141 gggtatagat ttgatgaggt caggccgtaa tcactgtcat tagccattga gatagcctct
     1201 tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc atgcataatc
     1261 gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccctct
     1321 accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc
     1381 tcaacccttt ccaacgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttca
     1441 gcggggttac caaattgaac cgcctgcatc gcttcaccca gccgattgac gaactgatca
     1501 taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc actattaatg
     1561 acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata
     1621 gctggtgctt taccccccaa ttccagacac actttggtga tgtttttcgc cgcagtcgcc
     1681 atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac ctttgggtta
     1741 cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa cacgccgcgc
     1801 ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg cgtaaattca
     1861 ctaggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt gcgggcaatg
     1921 aggaagaacg ggaagttcca cggcagaatg ccggtagtca caccaagcgc acgtttaaac
     1981 aaaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta acgccgtgcc
     2041 cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc agccagctgc
     2101 tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg ttcgcggatc
     2161 ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc ttcccattct
     2221 ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg accatcgggt
     2281 atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc gtctccacgc
     2341 caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga catgggcgac
     2401 tcctgtgatt tatatgtttg ttcttggtaa attcattgtt aattaacatc aatcatcata
     2461 gagacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag tgtaaaagga
     2521 ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg gttcacaaac
     2581 gggcaatttt tgacgtaacg agcgactttc catcgcctcg atgagcaccg catcgtgttt
     2641 aagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg
     2701 cccgcgggga ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag
     2761 tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga ttacactgtc
     2821 tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg
     2881 attgtcccca gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg
     2941 aacaacgata gccgtatgaa ctcgttctac ggcctgatta aatagcgcga tgctaaagcg
     3001 tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatgtg
     3061 ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag tggtgcggat
     3121 agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat
     3181 accaccacta atcagtaaag gaatttgctg atcgcgagca atcttacatg ccgcgtcgat
     3241 agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc
     3301 ggagaaatca tcctgcgcca gccactgtcc gataacattt atggcatcta tagttgccgg
     3361 cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct tatcgttccc ttaagaatac
     3421 tgcgtgagat aatagtgaga agctgagcac tctgaaagga ggaagtcgta tgtccttaac
     3481 cgccactccg caagcctgac atatctttta aggaaatgct gtagataaac attattcatc
     3541 ttgcagcaga gaattgtatt tttctgaatt cctgaatttt tacccttacc gttttagaat
     3601 taataaaaaa cccggcgcta aatgcttacc gggttttaga tttattaggc aaatagcagg
     3661 gttaaccgct aatctgctcc atcgcctgca aaatacgctt atctgaaatc ggataaggcg
     3721 taccaagttg ttgagcgaag taactaacgc gcagctcttc tatcatccaa cggatctctt
     3781 tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct
     3841 gctggacgtt ttcgactttc agcatctgtg cacgatcgcg atgtggatca accgccagtt
     3901 tttccagtcg tttttcaatc gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc
     3961 cgttaccagt gacaaaaccg cgatatacca acccgcccat ctgcgcttta atgtcagaaa
     4021 gccccagtgc catggtcata tccacccgcc ctttcagacg tttgttgata ttgaacactg
     4081 ccgtaagaat ttgctcgacc tgcttcgcaa tatctaccac cgtgtcgttc agttcggcac
     4141 gcactttctc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca ccattggcgt
     4201 cgatcagttg atccacaccg caggagatac agtcgtcgat cagctccagc actttgccat
     4261 acgggttgaa gtacagtccc agcttggctt tgttcggtaa cttttcatgt aaatacttga
     4321 tcggcgaggg aatatttagc agcagtagac ggcgaagacc attccacatt gcctgttttt
     4381 gctccagcgg gttatcaaac agtttgatcg ccacgctgtc gcgttcatcc accagcgccg
     4441 gccacgcctt cactttatag ttgccacgct tctgttcgta actttccggc agctgaccaa
     4501 aactccagat atgtaagccg ctctgctcga taccgtcatc cgccaccgcg gacagcgttt
     4561 cctgcacttt gcctttcagc gcatctttca gatcttgtag cgagcgccct tcttttagct
     4621 tcttgttttt gtcatccacc acgcggaagg tgattttcag gtgatcgggc acctgatccc
     4681 agtgccagtc ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc
     4741 tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg
     4801 gtgcaggcac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga gcaatcacca
     4861 attcgcggcg cagaccgggg atctgccatt caaacccgct ttcctcaacc tggttaagta
     4921 acggcagcgg aatatgtacg gtcacaccgt ccgcgtctgc gcctggctca aactgatagc
     4981 tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc ttgctgattt
     5041 tttccgcccc ttctttgatc aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc
     5101 ggctgacttt tttccaccag ctatcgaagt gacgagcgga gattacatcg tggctgatgc
     5161 gctggtcgta gaactcaaac agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt
     5221 tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac
     5281 gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacga cataacgccg
     5341 gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca taaacagtga
     5401 ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgcggttcg ctgtaggtac
     5461 gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc gcagcaatgc
     5521 gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc ggcggttttt
     5581 tgaataaacc agaaccgggg aagatggaga aacgcgcgtt acgtgcgccg gtatattctt
     5641 gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac gcaatgtgaa
     5701 tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc accacctgac
     5761 gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga taatcggtac
     5821 gacacaggcg acggaaggcg tttgaagaaa gcgccttttg ctgctcgcca agataattcc
     5881 acagatttac aaacgcgagg aagtcggatt ctttgtcgtg gaagcgacga tgtttttcgt
     5941 ccgatgcctg ctgtttgtcc atcggccgct cacgcgggtc ctgaatggag agcgcggacg
     6001 tgataatcat cgcctcacgc acgcagccat gtttctgcgc ttccagcacc atacgcgcca
     6061 gacgaggatc gaccggcagt tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc
     6121 tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg ccatcctgga
     6181 tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc agccccagcg
     6241 cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga tcggtaaact
     6301 ccgggcgcga gaggaaatcg tcttcggaat agagacgaat acagatccct tcggacacac
     6361 gaccacaacg gcctttacgc tgattggcag acgcctgtga aatgggttca atcggcaaac
     6421 gctgcacttt ggtgcgatag ctgtagcggc tgatacgcgc cgtaccgggg tcgataacgt
     6481 atttaatccc cggtacagtc agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgtc
     6541 gcccgctgtg cgactggaac acgcggttct gttcgctatt cgaaagccgc gcataaagcg
     6601 gcaagatttc ggtatggcgt aagttcagct tgttcagcgc atcggcggta tcgcgaattt
     6661 cccgctcgcc gctcataaag atcagaatgt cgccagggct ttcctggctc agttcgtcta
     6721 cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc atcggcttct tcaacaatcg
     6781 ggcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc ggcgcattat
     6841 taaagtggcg agaaaagcgt tccgggtcga tagtcgcgga agtgataatg atttttaggt
     6901 caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata ttcaggctgc
     6961 gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg tcttgctgga
     7021 tctccgccag caggataccg tcggtcatca gcttgaccat cgtgttatca cttacgtgat
     7081 cgctgaaacg cactttgtaa ccgatgcaac cgcctggctc cgttttcagc tcttccgcaa
     7141 tacggttcgc caccgttctt gccgccagac gacgcggctg ggtatggccg atcagtcctt
     7201 taatcccgcg ccccagctcc atacagattt tcggtagctg ggtcgtttta ccggaaccgg
     7261 tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagaata tcctgttttt
     7321 tctgactaac tggcaaattg tccggataag tgatttccgg tcgtgccgct tcacgcagca
     7381 ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg
     7441 catcaggatt tttaaccttc ttaacaccat gcagacggcg agaaaaacgc agtctgtcgc
     7501 gcagcatcaa cgaatccagc cgctgctgca aagcagtaaa ggtcaatttt tgttgttctg
     7561 tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga tagatataga
     7621 gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc ttcgatttat
     7681 tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg gcatcgccaa
     7741 ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc cagcatcctg
     7801 gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag
     7861 cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccgattcc ggtactggat
     7921 ggcgaactgg ttggcgctct gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa
     7981 gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt tatcgttatt
     8041 gcggcaccga tgtataactt caacatctca actcagttga aaaactattt tgacctggtt
     8101 gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct ggtaacgggt
     8161 aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc aacggacctg
     8221 gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt gaaatttgtc
     8281 ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa
     8341 gcggccatcg acagcattgt tgctgcataa ccggacacga tcttgatgtg gactgtagtt
     8401 cagtccacat ctcaatccac ttacctgttt tcccataatt tcagcatcgc tttgtgatct
     8461 tccttaagca caatatgcga acgtctggct cgaatataac taatcgcttc atcaacggtt
     8521 ttacagtgtc cgtaacataa caaccatgcc gccactacca gcgcactgcg cgataatccc
     8581 aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag catcgccacg
     8641 gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac acagaaatag
     8701 agtcgatctt ttgtcgctcg tccccgaggg aattcaaagg tgacgtccag aaccgcattc
     8761 tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtaat tttgctcacc
     8821 ggctccaggc gacgacaaaa ccaacgcata gacagccaca tcccgatgcg ccagggcaat
     8881 gtcagccagt aaacggcggg cgggagtttc ccctgactat ctttccctgt tgttatcgcg
     8941 ccaagcccac cgtacccacg gccaataatg agtagcgata atactggcca acataaccag
     9001 actggccacc ataactgaat catcaccatt agctccatca acacaatgca cgagcccgcg
     9061 cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg ataattccaa
     9121 cgacgatcga cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca
     9181 tcaataaaat gatgctgcca ggtcgtcaga gtagaaatag cgatgagtaa aaaccatccg
     9241 ccacagactt tacgccacct ctcagccaga tgctgacgaa agtgacgcca aagtagccag
     9301 cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc
     9361 gaaaatggcc atcccgtcac cccactcact tcaggacgga taaaactaaa cttcagcgga
     9421 tagagcaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg
     9481 cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc catataaaag atccagactc
     9541 cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga
     9601 ctggggatat catggctgtt aaggtcctga accgcggtga actgattaag agatccatag
     9661 gtgaagaaaa aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc
     9721 cgacgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatacccc actcatcaat
     9781 ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct ccccttgcga
     9841 acgcacgcgc attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcat
     9901 ctccagttga gggtgccacg gctgcccggt gtagatgaga atgccgaccg gttcgatggc
     9961 attggcaaga cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatacag
    10021 tccagagaca attgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa
    10081 cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg
    10141 cccctgtgca acatttaact cgctgtaatc acgtaacaaa atatcgctta cggcaggctc
    10201 gtttgccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa ccacgcggac
    10261 ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagtattt gtaaatgggt
    10321 tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt cgattaagcg
    10381 cccgaatgcg ttactacctt gcggttgatt ttgatagaca taatccagcg aactgcctga
    10441 atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgtcccga acaatttcat
    10501 cgcctttcgc ataaagcgat acgctaaatc aaccggcgat aatagcacgg gtccaccaga
    10561 aagtagccgc cattgatccg ctgatggtcc tgtgcagtct tcatgctgat aatcaaattt
    10621 ttgcgatttg ttagcatata aacgactaat aaagctttgc attttttcaa atgccagcgc
    10681 cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttcag
    10741 agggctacgt aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc
    10801 gcctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatctctaa taatacgttc
    10861 agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc
    10921 attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga
    10981 attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg
    11041 cacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa ttgcaggcgc
    11101 ataatcatga acctatgtgg caaccagcac tgcgccgacg ctttgcgcga tcacaaccac
    11161 ctcttccagt ccaacctagc tgtcgctggc agcaaaacgg acaaattcat cgacatcctg
    11221 cactgaacgc gcaagagatg ggctataacc acgcggcccc gaagtttttc catgcccccg
    11281 agcatcccat gcataaaaag cagtatctgg catcgccagt tcatcaacga tatgttgtag
    11341 acgaccagaa tgttcatggc cacgatgaaa taagacaatg acctt
//
LOCUS       DAJVOY010000130        11299 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVOY010000130 DAJVOY010000000
VERSION     DAJVOY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.130"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            292..1269
                     /gene="csiD"
                     /locus_tag="OQL90_003013"
     CDS             292..1269
                     /gene="csiD"
                     /locus_tag="OQL90_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1369707.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1289..2557
                     /gene="lhgO"
                     /locus_tag="OQL90_003014"
     CDS             1289..2557
                     /gene="lhgO"
                     /locus_tag="OQL90_003014"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1369708.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFNDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            2580..4015
                     /gene="gabD"
                     /locus_tag="OQL90_003015"
                     /pseudo
     CDS             2580..4015
                     /gene="gabD"
                     /locus_tag="OQL90_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4029..5295
                     /gene="gabT"
                     /locus_tag="OQL90_003016"
                     /pseudo
     CDS             4029..5295
                     /gene="gabT"
                     /locus_tag="OQL90_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5533..6894
                     /gene="gabP"
                     /locus_tag="OQL90_003017"
     CDS             5533..6894
                     /gene="gabP"
                     /locus_tag="OQL90_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1369709.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPVKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            6953..7615
                     /gene="csiR"
                     /locus_tag="OQL90_003018"
     CDS             6953..7615
                     /gene="csiR"
                     /locus_tag="OQL90_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1369710.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7616..8065)
                     /gene="kbp"
                     /locus_tag="OQL90_003019"
     CDS             complement(7616..8065)
                     /gene="kbp"
                     /locus_tag="OQL90_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1369711.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8149..8307)
                     /locus_tag="OQL90_003020"
     CDS             complement(8149..8307)
                     /locus_tag="OQL90_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1369712.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8490..8789
                     /locus_tag="OQL90_003021"
     CDS             8490..8789
                     /locus_tag="OQL90_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1369713.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8799..9323
                     /gene="ygaP"
                     /locus_tag="OQL90_003022"
     CDS             8799..9323
                     /gene="ygaP"
                     /locus_tag="OQL90_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1369714.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9370..9774)
                     /gene="stpA"
                     /locus_tag="OQL90_003023"
     CDS             complement(9370..9774)
                     /gene="stpA"
                     /locus_tag="OQL90_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1369715.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10442..10891
                     /gene="alaE"
                     /locus_tag="OQL90_003024"
     CDS             10442..10891
                     /gene="alaE"
                     /locus_tag="OQL90_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1369716.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGIYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(10928..11272)
                     /locus_tag="OQL90_003025"
     CDS             complement(10928..11272)
                     /locus_tag="OQL90_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1369717.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT         2666 a         2857 c         2981 g         2795 t
ORIGIN      
        1 ttagtttact aacggtcatt ttgcagtgaa gccatttact gttttttatc gaccagataa
       61 tctgttctct aatgttaact ccccctaacc tgttgcttta gttattcatt tcctgtctca
      121 ctttgcctta ataccctacg ttaaatgtta ctaatttgtt gtttttgatc acaataagaa
      181 aacaatatgt cgcttttgtg cgcatttttc agaaatgtag atatttttag attatggcta
      241 cgaaatgagc atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca
      301 ctgatcgccg tacaaaataa cgctgtcgat tcaggccagg actatagcgg attcactctc
      361 atcccgtcgg cgcaatcccc gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa
      421 cagtttctcg aacaggttgc cgaatggccc gtgcaggcgc tggagtacaa atcgtttctg
      481 cgttttcggg taggcaaaat tcttgacgat ctgtgtgcga atcagctgca accattgctg
      541 ttgaagcccc tgctaaaccg tgctgaaggt gcgctgttga tcaatgcggt gggtatcgat
      601 gatgtggcgc aggcagatga gatggtgaag ctggcaacgg cggtagcgca tctgattggt
      661 cgctcaaatt tcgatgcgat gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtc
      721 gataactcag acagctatct gcgtcagccg caccgcgtga tggaactgca caacgacggc
      781 acgtatgtcg aagagatcac cgattacgtg ctgatgatga aaatcgatga gcaaaacatg
      841 cagggtggaa attcgttgct gctgcatctc gatgactggg aacatctgga ccactatttc
      901 cgccatccac tggcgcgtcg cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc
      961 aaagatgttt tccatccagt gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat
     1021 atcgaccagt tcgtccagcc aaaagatttt gaagaaggcg tgtggttgag cgagctttcc
     1081 gatgccattg aaaccagcaa aggcattctt tctgtacccg ttcccgttgg caaattcctg
     1141 ttgataaaca acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg
     1201 cgccgtgaac tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg
     1261 catcagtaag cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat
     1321 catcggcatg tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt
     1381 gctggaaaaa gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca
     1441 tgccggggtc tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cgggaaaccg
     1501 cgccaccaaa gccttttgcg atcaaaacga cattcgctat gacaactgcg gcaagatgct
     1561 gatcgccacg tccgatctcg aaatggaacg gatgcgcgcg ttgtgggaac gcacggcggc
     1621 gaacggtatc gagcgcgagt ggttaaacgc cgaggaactg cgcgagcgcg aaccgaatat
     1681 caccgggctc ggcggcattt ttgtgccgtc cagcggcatt gtcagctatc gcgaagtcac
     1741 agcggcaatg gcaaaaatct tccaggccag aggcggtgag attatttata acgccgaagt
     1801 cagcgccctc agtgagcata aaaacggcgt ggtcgtccga acccgtcagg gcggcgaata
     1861 tgaagcatcg acgctgatta gctgttccgg gctgatggct aaccggctgg tgaaaatgct
     1921 cggcctcgaa ccgggtttta ttatctgccc gttccgtggc gagtatttcc gccttgcgcc
     1981 ggagcataac cagattgtta accatctgat ttaccccatc cctgacccgg caatgccgtt
     2041 tttgggcgtg catctcaccc gcatgatcga cggcagcgtg accgtcgggc caaacgcggt
     2101 gctggctttt aaacgcgaag gctatcgcaa gcgcgacttc tcatttaacg acacgctgga
     2161 aattttaggc tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg
     2221 cgagatgaaa aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc
     2281 ccggctttcg ttaagcgatc tccagccgtg gcccgccggt gtgcgggcgc aggcggtatc
     2341 gccggacggc aagctgattg acgattttct gtttgtcacc accccgcgca cgatccacac
     2401 ctgcaatgcg ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag
     2461 caaggtacaa acgctgttgg caagccagag taaccccgga cgcacgctgc gagcgacacg
     2521 tagtgtggat gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa
     2581 tgaaacttaa cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg
     2641 acgccaacaa tggcgggatc atcgacgtca ccaatccggc gaacggcgac aagctgggta
     2701 gcgtgccgaa aatgggcgct gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc
     2761 tgcccgccag gcgtgcgctc accgccaaag aacgcgccaa cattctgcgc aactggttca
     2821 atttgatgat ggagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggcaaac
     2881 cactggccga agcgaaaggc gaaatcagct acgctgcctc ttttattgag tggtttgccg
     2941 aagaaggcaa acgtatttat ggcgatacca ttcccggtca tcaggccgat aagcgcctga
     3001 ttgttatcaa gcagccgatt ggcgttaccg cagctatcac gccgtggaac ttcccggcgg
     3061 cgatgattac ccgtaaagcc ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc
     3121 ccgccagtca gacaccgttc tctgcgctgg cgctggcgga gctggcgatt cgcgcgggca
     3181 ttgcggctgg cgtgtttaac gtggtcaccg gttcggcagg cgcggtcggt aatgaactga
     3241 ccagcaaccc gctggtacgc aaactgtcgt ttaccggttc gaccgaaatt ggccgacagt
     3301 taatggaaca gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc
     3361 cgtttatcgt ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga
     3421 aattccgcaa cgccgggcaa acctgcgtct gcgccaaccg tttggcgtgt atgaccgctt
     3481 tgccgaaaaa ttgcagcagg cggtgagcaa actgcacatc ggcgacgggc tggataaagg
     3541 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     3601 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcgct aaagcggatg aacgcggcgg
     3661 caacttcttc cagccgacca ttctggtgga cgtgccggcc aacgccaaag tgtcgaaaga
     3721 agagacgttc ggccccctcg ccccgctgtt ccgctttaaa gatgaagccg atgtgattgc
     3781 gcaagccaat gacaccgagt ttggccttgc cgcctatttc tacgcccgtg atttaagccg
     3841 cgtcttccgc gtgggcgaag cgctggagta cggcatcgtc ggcatcaata ccggcattat
     3901 ttccaatgaa gtggccccgt tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc
     3961 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     4021 gaatgcgaat gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     4081 gcgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     4141 ttgaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc
     4201 tgcatccgaa ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct
     4261 tccaagtgct ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     4321 cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg
     4381 cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgcgt
     4441 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     4501 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     4561 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     4621 aagatatcgc cgccatcgtg attgagccgg ttcagggcga aggcggtttc tacgccgcgt
     4681 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     4741 ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcggtg gagcagatgg
     4801 gcgtggcgcc ggatcttacc acctttgcga aatcgatcgc tggcgggcgt caccgggcgc
     4861 gcggaagtga tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg
     4921 attgcctgcg tggctgcgct ggaagtgttg aaggtgttcg agcaggaaaa tctgctgcaa
     4981 aaagccaacg atctggggca gaagctaaaa gacggattgt tggcgatagc cgaaaaacac
     5041 ccggagatcg gcgacgtacg cgggctggga gcgatgattg ccatcgagct gtttgaagat
     5101 ggcgatcaca gcaaaccgga cgcaaaactc accgccggga tcgtggcacg cgcccgcgat
     5161 aagggcctga ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtactg
     5221 ctcaccattg aagacgctca gatccgtcag ggtctggaga ttatcggtca gtgttttgat
     5281 gaggcgaagc agtagcaccg tccccatgca ggaggcgacg ctgcgcgtct tgtccggcct
     5341 acgagaatcc aggtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg
     5401 aataaatcta tgtcggccgg ataatgcgga gccgctctcc ggcaaaaaga atcaataaca
     5461 attaaacgcg tgacccggcg cgggaaatgt cggggcgctc tcccaagtga cacactttcg
     5521 agaggattca ggatggggca atcatcgcaa ccacatgagt taggcggcgg gctgaaatca
     5581 cgccacgtca ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc
     5641 agcgtcgcca tcgccgaagc gggcccggca gtattactgg cctatctgtt cgccgggcta
     5701 ctggtggtta tgattatgcg gatgttggcg gaaatggcgg ttgccacgcc cgataccggt
     5761 tcgttttcca cctatgccga taaagccatt ggtcgctggg cgggctatac catcggctgg
     5821 ttgtactggt ggttttgggt actggttatc ccgctggaag ccaacatcgc cgccatgatc
     5881 ctgcactcgt gggttccagg cattcccatc tggttatttt ccctcgtcat tacccttgcc
     5941 ttaactggca gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg
     6001 ctgtgcaaag tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt
     6061 ttttaccctt atgccgaagt gagcgggatc tcaagattgt gggatagcgg cggctttatg
     6121 cccaacggct tcggtgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc
     6181 gcagaaattg tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgtccgc
     6241 gccactaact cggttatctg gcgtatttct atcttctatt tgtgctctat ttttgtcgtc
     6301 gtggcattaa ttccgtggaa tatgcccgga ctaaaagccg tcggttctta tcgctcggta
     6361 ctggaattgc tcaatattcc ccatgcgaaa ttaatcatgg actgtgtgat attactttcc
     6421 gtaaccagct gcctgaactc ggcgctgtat accgcttcca ggatgctcta ctccttaagc
     6481 cgtcgcggag atgcgcctgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg
     6541 gcggtgttac tctccaccgg cgcggcattc ttaacggtgg tggtgaacta ttacgtgcct
     6601 gtgaaggtat ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta
     6661 gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt
     6721 cgcttgcgca tgtggcttta tccctggctc acctggctgg tcatcggctt tattaccttt
     6781 gtgttggtag tgatgctatt ccgcccggcg caacagttag aagtgatctc caccgactta
     6841 ttagcaatag ggattatctg taccgtgcca attatggcgg ctggaataag ctgataatgt
     6901 ggcaaaaaac acccgttcat aatacgcgct gataataatc aggagtcaca ccatgaccat
     6961 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
     7021 ggatgaaaaa ttacggatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt
     7081 gcgggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
     7141 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgatg cccgcgccaa
     7201 tatggaagcg atgttagtga gtctggcgat tgcccgcggt agcgatgagt gggaggcaga
     7261 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
     7321 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
     7381 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gctatcgatt
     7441 tatctggctg cggcgaacgg tgctttcggt agaaatgctg gaggataaac acgatcagca
     7501 ccagaccctg actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg
     7561 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
     7621 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
     7681 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
     7741 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
     7801 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
     7861 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
     7921 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
     7981 tcgcctgatc gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat
     8041 ctttcacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
     8101 tgtagcagag gaacgcggcg cgttaacgcc gcggggggga gcacaggttt aatcgcgggt
     8161 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
     8221 aataatgaac gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat
     8281 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
     8341 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta
     8401 cgcttttact ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt
     8461 tacttaattt agaaaatact taaataaata tgactgaact cgcgcaatta caggccagtg
     8521 ccgaacaggc agcggcctta ttgaaagcaa tgagccatcc taaacgattg ctgattctgt
     8581 gcatgcttag cggttccccc ggcaccagcg cgggagagct gacgcgcatt accggactga
     8641 gtgcctctgc gacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc
     8701 aacgggatgc ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg
     8761 ccaccctgaa aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc
     8821 cgcatgatgc acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgacgctg
     8881 atgaatatct tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac
     8941 agtcaggtct tccacctaaa ttacgtcgcg agcaaattat attccactgc caggcaggta
     9001 aacgcaccag taataacgcc gataaattag cagcgattgc cgcccccgca gaaatatttt
     9061 tactcgaaga taggattgat ggctggaaaa gagctggatt gccagtagcg gtaaataaat
     9121 ctcaaccctt gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg
     9181 gcgttgtact gggttatacc gtaaatagcg gtttcttctt attaagtggc tttgttggtg
     9241 ccgggttact gtttgcagga atcagcggtt tttgtggaat ggcaaggttg ttagataaga
     9301 tgccgtggaa ccaacgagct tgagaagcga cgccggacgc gccctagcag cgacatccgg
     9361 cctcagtaat tagatcagga aatcgtcgag agatttacct tctgccagcg cctgagcaat
     9421 tggcttcggt gtacggccct gaccggtcca ggtcttagtt tcaccgttaa catcagtgaa
     9481 tttatatttc gccggacgcg gctggcgttt tttaccagca cgtggtgcag cagcagagct
     9541 attacccaat aactcttccg ggttaattcc gtcagctttc atcagctcca gccaggtgct
     9601 aattttttcc tgacgctcag ccagttcacg ctgctgctgt tcttcttctt cacgtctttc
     9661 tttagtgaca accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg
     9721 agccatcgca cggagggttc gaatgttatt taaactttgt aacattacgg acataataaa
     9781 gaaaaccttt taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta
     9841 ttatatttcc acaaaattta atctatttac agtcctgcgc gagattattt cagctgttat
     9901 tttctgaaac tctcagaata atttcaagat tatatattag ccattttgat gagtaattta
     9961 ggttattggt gaacagagtg gcgctctctg ctcgctaact ccgcaagcct gataaaaccg
    10021 aagtgaataa aaatgcaata taacagaata aaagaaaaca cccctcactg gtgctttctg
    10081 ctgttaatgt acattttctg gcatcaaaaa tgctgtaaat gacccggtca cgacaaacac
    10141 cgaattctct acgcttatag cgttacctca cccccaaact ttcattagtg aaattaaagg
    10201 caaggtcata tccaattgat ttaattgaaa ataaatcata tcggcatgta aaaatccaca
    10261 atgtacaaaa aacgagctgt tacggaatat tttatctaca aaaaactgac taaataaaag
    10321 tttttcacta attgattagt catagccagc gatatacgct atgcgaaaat gcagatggca
    10381 atgagatcca ctgctttcat ctccattaac atcccattac gctttattaa ggagcattag
    10441 catgttctca ccgcagtcac gcttgcgtca tgcagttgca gatacgttcg cgatggttgt
    10501 ttactgttct gtcgtgaaca tgtgtattga agttttcctc tccggaatga gcttcgaaca
    10561 gtctttttat tccagattgg tagcgattcc ggtgaacatc ttaattgcat ggccatacgg
    10621 tatataccgc gatctgttta tgcgcgcggc acgcaaagtt agcccgtcgg gctggataaa
    10681 aaatctggct gatatcctgg cttatgtgac gttccagtca ccggtgtatg tggcgatctt
    10741 gttagtggtg ggcgcagact ggcatcagat tatggcggcg gtcagttcaa acatcgttgt
    10801 ttcgatgttg atgggggcgg tttatggcta cttcctcgat tattgccgcc gactgtttaa
    10861 agtcagccgt taccagcagg taaaagcctg actcttcttt tcgcgactgg catcgccagt
    10921 cgcgtcttta actggcttcg ccaaataaac cattcaaata acgttcaagc gcaacacgcg
    10981 aactgaagcc atgcgggata ccgtaatgct cgtgtcgctc gcctgctaaa tagagcggaa
    11041 attgctggta atgatcgcaa gttttaatat cggaagcggg ttcagccagt gttgaactgc
    11101 gttcttttaa tgttggatga attaccagag cggtacgtcc cattcgcgct tcgcgattaa
    11161 catagacata attttcccca cggcggtaac catacgcttt ctgggttacc acatcgacag
    11221 taaaaccgac tttttcaagt acgcgcgcca cctcgtctgg tcgtaaatac atatttgatc
    11281 ctcgttattt tcaaccgcg
//
LOCUS       DAJVOY010000131        11250 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVOY010000131 DAJVOY010000000
VERSION     DAJVOY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.131"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..690
                     /gene="hdhA"
                     /locus_tag="OQL90_003026"
                     /pseudo
     CDS             1..690
                     /gene="hdhA"
                     /locus_tag="OQL90_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
     gene            919..1509
                     /gene="uidR"
                     /locus_tag="OQL90_003027"
     CDS             919..1509
                     /gene="uidR"
                     /locus_tag="OQL90_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1369718.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1897..3708
                     /gene="uidA"
                     /locus_tag="OQL90_003028"
     CDS             1897..3708
                     /gene="uidA"
                     /locus_tag="OQL90_003028"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS1369719.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLEWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3705..5078
                     /gene="uidB"
                     /locus_tag="OQL90_003029"
     CDS             3705..5078
                     /gene="uidB"
                     /locus_tag="OQL90_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCS1369720.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5117..6382
                     /gene="uidC"
                     /locus_tag="OQL90_003030"
     CDS             5117..6382
                     /gene="uidC"
                     /locus_tag="OQL90_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1369721.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6608..8116)
                     /locus_tag="OQL90_003031"
     CDS             complement(6608..8116)
                     /locus_tag="OQL90_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1369722.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8217..9392)
                     /gene="manA"
                     /locus_tag="OQL90_003032"
     CDS             complement(8217..9392)
                     /gene="manA"
                     /locus_tag="OQL90_003032"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1369723.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9591..11237
                     /gene="fumB"
                     /locus_tag="OQL90_003033"
     CDS             9591..11237
                     /gene="fumB"
                     /locus_tag="OQL90_003033"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1369724.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2806 a         2632 c         2903 g         2909 t
ORIGIN      
        1 aatcgccatt acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga
       61 cgcagctaac catgttgtag acgaaattca acaactgggg gggcaggcat ttgcctgccg
      121 ttgtgatatt acttccgaac aggaactctc tgcactggca gatttgctat cagtaagctg
      181 ggtaaagttg atattctggt taacaacgcc ggtggcggtg gacctaaacc gtttgatatg
      241 ccaatggcgg attttcgctg tgcttatgaa ctgaatgtgt tttctttttt ccatctgtca
      301 caacttgttg cgccagaaat ggaaaaaaat ggcggtggcg ttattctgac catcacttct
      361 atggcggcag aaaataaaaa tataaacatg acttcctatg catcatctaa agctgcggcc
      421 agtcatctgg tcagaaatat ggcatttgac ctgggtgaaa aaaatattcg ggtgaatggc
      481 attgcgccgg gggcaatatt aaccgatgcc ctgaaatccg ttattacacc agaaattgaa
      541 caaaaaatgt tacagcacac gccgatcaga cgtctgggtc aaccgcaaga tattgctaac
      601 gcggcgctgt tcctttgctc gcctgcagcc agctgggtaa gcggacaaat tctcaccgtc
      661 tccggtggtg gggtacagga gctcaattaa tacactaacg aaccggtaaa cagccgtatg
      721 tgttgtttac cgggatagcg tgtgcaattc atcaacgtct ctgctaaaca actggcagcc
      781 aaatcacggc tattggttaa ccaatagctg gatgaaaagc atacgaatag agtgtgcctt
      841 cgcactattc aacagcaatg ataggcgctc acctgacaac gcggtaaact agttattcac
      901 gctaactata atggtttaat gatggataac atgcagactg aagcacaacc gacacggacc
      961 cggatcctca atgctgccag agagattttt tcagaaaatg gatttcacag tgcctcgatg
     1021 aaagccatct gtaaatcctg cgccattagt cccgggacgc tttatcacca tttcatctcc
     1081 aaagaagcct tgattcaggc gattatctta caggaccagg agagggcgct ggcccgtttc
     1141 cgggaaccga ttgaagggat tcattttgtt gactatatgg tcgagtctat tgtctctctc
     1201 acccatgaag cctttgggca acgggcgctg gtggtcgaaa ttatggcgga agggatgcgt
     1261 aacccacagg tcgccgccat gcttaaaaat aagcatatga cgatcactga atttgttgcc
     1321 cagcggatgc gtgatgccca gcaaaaaggc gagataagcc cagacatcaa cacgtcaatg
     1381 acttcacgtt tactgctgga tctgacctac ggtgtactgg ccgatatcga agcggaagat
     1441 ctggcgcgtg aagcgtcgtt tgctcaggga ttacgcgcga tgattggcgg tatcttaacc
     1501 gcatcctgat cctctcattt cgcgggctgg tgataactgt gcccgcgttt catactttaa
     1561 tttccctgta caaaatacaa tctagccggg atcggagaat tcccccccaa aatattcacc
     1621 gtagccatat gtcatgagag tttatcgttc tcaagatgcc cgaacgaacg ttcgattgct
     1681 tattttgtgg cttctgtaaa cgctgtttta aagattaatg cgatctatat cacgctgtgc
     1741 gtattgcaga ttttggtttt ttgatcgcgg tgtcagttct ttttatttcc atttctcttc
     1801 catgggtttc tcacagataa ctgtgtgcaa cacagaattg gttaactaat cagattaaag
     1861 gttgaccagt atttttattt gaatgaggag tcccttatgt tacgtcctgt agaaacccca
     1921 acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt
     1981 ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca
     2041 ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgtggg caacgtctgg
     2101 tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc
     2161 gatgcggtca ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag
     2221 ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta
     2281 cgtatcaccg tttgtgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg
     2341 attaccgatg aaaacggcaa gaaaaagcag tcttacttcc atgatttctt taactatgcc
     2401 gggatccatc gcagcgtaat ggtctacacc acgccgaaca cctgggtgga cgatatcacc
     2461 gtggtgacgc atgttgcgca agactgtaac cacgcgtctg ttgactggca ggtggtagca
     2521 aatggtgatg tcagcgttga actgcgtgat gcggatcagc aggtggttgc aactggacaa
     2581 ggtaccagcg ggactttgca agtggtgaat ccccacctct ggcaaccggg tgaaggttat
     2641 ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta cccgctgcgc
     2701 gtcggcatcc ggtcagtggc agtgaaggac gaacagttcc tgattaacca caaaccgttc
     2761 tactttactg gctttggtcg tcatgaagat gcggatttgc gaggcaaagg attcgataac
     2821 gtgctgatgg tgcacgatca cgcattaatg gactggattg gggccaactc ctaccgaacc
     2881 tcgcattacc cttacgctga agagatgctc gagtgggcag atgaacatgg catcgtggtg
     2941 attgatgaaa ctgcagctgt cggctttaac ctctctttag gcattggttt cgaagcgggc
     3001 aacaagccga aagaactgta cagcgaagag gctgtcaacg gggaaactca gcaggcgcac
     3061 ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg
     3121 agtattgcca acgaaccgga tacccgtccg caaggtgcac gagaatattt cgcgccactg
     3181 gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc
     3241 tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct gaaccgttat
     3301 tacggatggt atgtccaaag cggcgatttg gaaacggcag agaaggtact ggaaaaagaa
     3361 cttctagcct ggcaggagaa actgcatcag ccgattatca tcaccgaata cggcgtggat
     3421 acgttagccg ggctgcactc aatgtacacc gacatgtgga gtgaagagta tcagtgtgca
     3481 tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta
     3541 tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg taacaagaag
     3601 gggatcttca cccgcgaccg caaaccgaag tcggcggctt ttctgctgca aaaacgctgg
     3661 actggcatga acttcggtga aaaaccgcag cagggaggca aacaatgaat caacaactct
     3721 cctggcgcac catcgtcggc tacagcctcg gtgacgtcgc caataacttc gccttcgcaa
     3781 tgggggcgct cttcctgttg agttactaca ccgacgtcgc tggcgtcggt gccgctgcgg
     3841 cgggcaccat gctgttattg gtgcgggtat tcgatgcctt cgccgacgtc tttgccggac
     3901 gagtggtgga cagtgtgaat acccgctggg gaaaattccg cccgttttta ctcttcggta
     3961 ctgcgccgtt aatgatcttc agcgtgctgg tattctgggt accgaccgac tggagccata
     4021 gcagcaaagt agtgtatgca tatttgacct acatgggcct cgggctttgc tatagcctgg
     4081 tgaatattcc ttatggttca cttgctaccg cgatgaccca acaaccacaa tcccgcgccc
     4141 gtctgggcgc ggctcgtggg attgccgctt cattgacctt tgtctgcctg gcatttctga
     4201 tagggccgag cattaagaac tccagcccgg aagagatggt gtcggtatac catttctgga
     4261 cgattgtgct ggcgattgcc ggaatggtgc tttacttcat ctgcttcaaa tcgacgcgtg
     4321 agaatgtggt acgtatcgtt gcgcagccgt cattgaagat cagtctgcaa accctgaaac
     4381 ggaatcgccc gctgtttatg ttgtgcatcg gtgcgctgtg tgtgctgatt tcgacctttg
     4441 cggtcagcgc ctcgtcgttg ttctacgagc gctatgtgtt aaatgatacc gggctgttca
     4501 ctgtgctggt actggtgcaa aacttggttg gtactgtggc atcggcaccg ctggtgccgg
     4561 ggatggtcgc gaggagcggt aaaaagaata ccttcctgat tggcgctttg ctgggaacct
     4621 gcggttatct gctattcttc tgggtttccg tctggtcact gccggtggcg ttggttgcgt
     4681 tggccatcgc ttcaattggt cagggcgtta ctatgactgt gatgtgggca ctggaagctg
     4741 ataccgtaga atacggtgaa tacctgaccg gcgtgcgaat tgaagggctc acctattcac
     4801 tattctcatt tacccgtaaa tgcggtcagg caatcggagg ttcaattcct gcctttattt
     4861 tgggattaag cggatatatc gccaatcagg tgcaaacgcc ggaagttatt atgggcatcc
     4921 gcacatcaat tgccttagta ccttgcggat ttatgctact ggcattcgtt attatctggt
     4981 tttatccgct cacggataaa aaattcaaag aaatcgtggt tgaaattgat aatcgtaaaa
     5041 aagtgcagca gcaattaatc agcgatatca ctaattaata ttcaataaaa ataatcagaa
     5101 catcaaaggt gcaactatga gaaaaatagt ggccatggcc gttatttgcc tgacggctgc
     5161 ctctggcctt acctctgctt atgcggcgca actggctgac gatgaagcgg gactacgcat
     5221 cagactgaaa aacgaattgc gcagggcgga taagcccagt gctggcgcgg gaagagatat
     5281 ttacgcatgg gtacagagag gattgttcga tttcaatagt ggttattatt ccaatattat
     5341 tggcgttgaa ggcggggcat attatgttta taaattaggt gctcgtgctg atatgagtac
     5401 ccggtggtat cttgatggtg ataaaagttt tggctttgcc ctgggggcag taaaaataaa
     5461 accctgtgaa aatagcctgc ttaaattagg tcgcttcggg acggattata gttatggtag
     5521 cttaccttat cgtattccgt taatggctgg cagttcgcaa cgtacattac cgacagtttc
     5581 tgaaggagca ttaggttatt gggctttaac accaaatatt gatctgtggg gaatgtggcg
     5641 ttcacgagta tttttatgga ctgattcaac aaccggtatt cgtgatgaag gggtgtataa
     5701 cagccagacg ggaaaatacg ataaacatcg cgcacgttct tttttagccg ccagttggca
     5761 tgatgatacc agtcgctatt ctctgggggc atcggtacag aaagatgttt ccaatcagat
     5821 acaaagtatt ctcgagaaaa gcataccgct cgacccgaat tatacgttga aaggggaatt
     5881 gctcggcttt tacgcgcagc tcgaaggttt aagtcgtaat accagccagc ccaatgaaac
     5941 ggcgttggtt agtggacaat tgacctggaa tgcgccgtgg ggaagcgtat ttggcagtgg
     6001 tggttatttg cggcatgcaa tgaatggtgc cgtggtggat accgacattg gctatccctt
     6061 ttcattaagt cttgatcgta accgtgaagg aatgcagtcc tggcaattgg gcgtcaacta
     6121 tcgtttaacg ccgcaattta cactgacatt tgcaccgatt gtgactcgcg gctatgaatc
     6181 cagtaaacga gatgtgcgga ttgaaggcac gggtatctta ggtggtatga actatcgggt
     6241 cagcgaaggg ccgttacaag ggatgaattt ctttcttgct gccgataaag ggcgggaaaa
     6301 gcgcgatggc agtacgctgg gcgatcgcct gaattactgg gatgtgaaaa tgagtattca
     6361 gtatgacttt atgctgaagt aagaaataac gccggagaga aaaacctccg gcgtttcaga
     6421 ttgttgacaa agtgcgcgtt ttttatgccg gatgcggcgt aaacgcctta tccagcctac
     6481 aaaaactcat aaattcaaag tattgcagga aaaggtaggc ctgataagcg tagcgcatca
     6541 ggcaatctct ggtttgtttt cagatgaaaa cgccgaagtg aaaattctcc ggcgttttgg
     6601 ccgtgaatta ctgctgcgga attgccggta cagccggaac gttaagagct ggcatcgcaa
     6661 acatgccaac aaaatcttct aacgacattt tctgcccatt taacgttatc tgaccgttag
     6721 tatattgcag gctggtggtg atggtattgt cctgcaaggt ggtcagacgg aacatctgcc
     6781 ccattgctga tgcaccttca acttgctgtt tcgccagttt tttcgcttga tcttcctgat
     6841 aaccttccag cttcgctacc tgagtcataa actcagttgc catatccacc ggaatggtca
     6901 gtttcgcatc cagagattta accgaacgat ctacttcctg cgccagcgtt tgcggcgctt
     6961 ctttagtcgt tgccggatct ttcaggaaca gcgacagatt cagcgcactt tcaccctgac
     7021 tgtttttcca gcttagcggc gcgacagtaa tcaccggatc gcctttcaac atcagcggca
     7081 gggcgctaaa gaaggcttcc gtcactttct cctgataaag ttcgggattg ttggcaattt
     7141 ctggctgtgc cagcagcgcc tgagtttgcg cgttatattg ctggctgaac tgatgccagg
     7201 cttcaccatc aatctggccg acttttaaag tcagcttgcc gctgcccaga tcctgattct
     7261 gtaccttcag gctgtttagc gagtaatcca gttggctatt gatcgtttta ccgtcattga
     7321 ccagatccga tttaccgctg atctccatgc cttccagcag tgccagttct ttgccttcca
     7381 ctgaaatggt cattttttcc agtgacagtt tttggtttcc tacgcgctca ccaaaacttg
     7441 ccagcgtgct ggaaccgtcg gttttcagat tattaaaggt caactgcact ttctggttgt
     7501 attcgttaac tgcgtctatc cgaccacttt gcgcctcccc ggaaagggag atggctttgc
     7561 cgtctctgtc agcatttaac tggaactcgc cgccgctaaa ggcgactttt tcatcctttt
     7621 gctcgtaatt cagtggcttg agcgaaatat cggaactgga atcaccgctg taaccaatgc
     7681 gcgagttaat ctcaaaaggc gtttcacctt ttgccatatc aaacagtggt ttgcttactt
     7741 cgttattaac cagcgtggtt tgaattgatg ccatcgacgg gatcaggttc agttttttaa
     7801 gctgggcaag cgggaaggga ccatgatcaa ccgattcgtt gaagatgacg ctctgaccgc
     7861 ttttaatcca cggattttct ttcccggcaa tgggtttcac caacagttgc aactggctgc
     7921 tgaatacgcc gcgatgatag ttttgataac tcacttccag gttggattca ggagctgtca
     7981 gttttagttg cgcgttcgcc tgcgcgacca tgtcttcgag atgggtttca atcttcttgc
     8041 ctgtatacca tgcgccgcct gtccagacta cgcctagcgc aacaatgacg cctaccgcta
     8101 ccagcgattt attcataatg attatccata aaatgaaatc aggcggactg gccgcctgaa
     8161 ggtgttataa gcctttaata agcttagcaa gagatgttaa ttttttcagt aagctcttac
     8221 agcttgttgt aaacacgcgc taaacggccg tggcctttga cagtcaccgg tgattcgttg
     8281 gcggcaataa acgctgattc acccggttta agctgtaact gctgagaacc tttccacaac
     8341 gttgcatcgc cttcgacgca gaacaaaatg gcggcactct gctggctaat ggtggtttct
     8401 ttatcactaa ggtcatgcag cgagaaggca aaatcatcca ctggaatcgg gaagtccagt
     8461 tctgcacctt gtttcaccgg ctgggtcaac aactggttag ccggtttggc ttcgaatttc
     8521 acattggcaa ccagttccgg aatatcaatg tacttaggcg tcagacccgc acgcagcacg
     8581 ttatcggagt tcgccatcac ttccagcgcc acgccttgca ggtaagcgtg cggtgtttca
     8641 gcgaacagga acatcgcttc gccaggattc aatttcacca cattcagtaa cagcggggag
     8701 aacaggccgc tgtcttccgg gtaaaattca gaaattaaac gaatcgtttg ccacggttca
     8761 ccctgctgac tgtcgagcgc cgattttaaa atcgccagcg cgcgggattt ttcttcaccc
     8821 tgcatattca acaggctggc gaacagttcg cttaaacgtt cggcattagg ctgttgtaaa
     8881 aagtgagcaa ttgccggatg tgcacctgcg actggctgga gtagggagac aatctcggaa
     8941 aactcacgaa acgcgttcat cgcaaggaaa ggcgtcagcg caaaaaccag ctccggcttg
     9001 tggttaggat ctttatagtt acgctcggcg gcatccatcg ggatacctgc ggcattttct
     9061 ttggcaaaac cgatttcaga attgcgtttg tttggatgaa cctgaatgga gagcggctgt
     9121 gctgcgcata atactttgaa caggaaaggc agttcgccaa agcgtttggc aacggcctct
     9181 ccgagcagag tcgatttatc actctcaatc acatcacgca gtgaaacgat atctccggcg
     9241 gcattctgca ctcgtgaact gcttttcgga tgtgcgccca tccacagctc ggccatcggc
     9301 tggctggacg gattttccat accataaagt tcagtcaacg ccgttttgct gccccaggca
     9361 tagttttgca ctgagttaat gagtttttgc atgatcaatc cctgttttaa tgtggaaatt
     9421 aatcccacta ttaaagcaag aatcctacgg gaagtaacct ggagccgcaa aaagtcgtac
     9481 tagtctcagt ttttgttaaa aaagtgtgta ggatattgtt actcgctttt aacagggcaa
     9541 cggaacaccc gcccagagca taaccaaacc aggcagtaag tgagagaaca atgtcaaaca
     9601 aaccctttca ttatcaggct ccttttccac tcaaaaaaga tgatactgag tattacctgc
     9661 taaccagcga acacgttagc gtatctgaat ttgaagggca ggagattttg aaagtcgcac
     9721 ccgaagcgtt aactctgttg gcgcgtcagg cgtttcatga tgcgtcattt atgctgcgtc
     9781 cggctcacca acaacaggtg gccgacattc tgcgtgaccc ggaagccagc gaaaatgata
     9841 aatatgtggc gctgcaattc ctgcgcaact ccgacatcgc ggcgaaaggt gttctgccaa
     9901 cctgtcagga taccggcact gcgattattg ttggtaaaaa agggcagcgt gtatggaccg
     9961 gtggctgtga tgaagcggcg ctggcgcgcg gtgtctataa cacttatatc gaagataatt
    10021 tgcgctactc gcaaaacgcg ccgctggata tgtataaaga ggtgaatacc ggcaccaatc
    10081 tgccagcgca gatcgatctt tatgccgttg atggcgacga gtacaaattc ctctgtatcg
    10141 ccaaaggtgg tggttcggca aacaagacgt atctctatca ggaaaccaaa gcgttactaa
    10201 cgccggggaa actgaaaaat tacctggttg agaagatgcg cacgctgggt acggcggcct
    10261 gtcctccgta tcatattgcg ttcgtaattg gtggaacttc tgcagaaact aaccttaaaa
    10321 cagtgaaact ggcttccgct aaatactatg acgaactgcc aacggaaggg aatgagcacg
    10381 gtcaggcatt ccgcgatgtg gaactggaaa aagaattgct gatcgaagcg caaaatctcg
    10441 gtctgggtgc gcagtttggt ggtaaatact tcgctcacga catacgcgtg attcgcctgc
    10501 cacgtcacgg cgcatcctgt ccggttggta tgggtgtttc ctgctctgct gaccgtaata
    10561 tcaaagcgaa gatcaaccgt caggggatct ggatcgaaaa actggaacat aatccaggta
    10621 aatatatccc tgaagagctg cgtaaagcgg gagaaggcga agcggtgcgc gttgacctta
    10681 accgtccgat gaaagagatc ctcgcacagt tgtcgcagta tcccgtttct acacgcttat
    10741 cgcttaacgg cacgattatc gtcggtcgtg atattgctca cgccaaactg aaagagcgga
    10801 tggataacgg tgaagggctg ccgcagtaca ttaaagatca tccgatttac tacgcgggtc
    10861 cggccaaaac gccggaaggt tatgcctccg gttctcttgg cccaacgacc gccggacgga
    10921 tggattctta tgtcgatcaa ctgcaagcgc agggcggaag tatgatcatg ctggcgaaag
    10981 gcaaccgcag ccagcaggtg acggatgcct gtaaaaaaca cggcggcttc taccttggca
    11041 gtatcggtgg tccggccgct gtattggcgc agggaagtat taagagcctg gaatgtgttg
    11101 aatatccgga actgggaatg gaagccatct ggaaaattga agtggaagat ttcccggcgt
    11161 ttatccttgt ggatgataaa ggaaatgact tcttccagca gatacaactc acacagtgca
    11221 ctcgctgtgt gaaataaaca gagccgccct
//
LOCUS       DAJVOY010000132        11187 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVOY010000132 DAJVOY010000000
VERSION     DAJVOY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.132"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(169..375)
                     /locus_tag="OQL90_003034"
     CDS             complement(169..375)
                     /locus_tag="OQL90_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1369725.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            687..776
                     /gene="kdpF"
                     /locus_tag="OQL90_003035"
     CDS             687..776
                     /gene="kdpF"
                     /locus_tag="OQL90_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1369726.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            776..2449
                     /gene="kdpA"
                     /locus_tag="OQL90_003036"
     CDS             776..2449
                     /gene="kdpA"
                     /locus_tag="OQL90_003036"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1369727.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALSADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2472..4520
                     /gene="kdpB"
                     /locus_tag="OQL90_003037"
     CDS             2472..4520
                     /gene="kdpB"
                     /locus_tag="OQL90_003037"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCS1369728.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4529..5072
                     /gene="kdpC"
                     /locus_tag="OQL90_003038"
                     /pseudo
     CDS             4529..5072
                     /gene="kdpC"
                     /locus_tag="OQL90_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5065..7749
                     /gene="kdpD"
                     /locus_tag="OQL90_003039"
     CDS             5065..7749
                     /gene="kdpD"
                     /locus_tag="OQL90_003039"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCS1369729.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7746..8423
                     /gene="kdpE"
                     /locus_tag="OQL90_003040"
     CDS             7746..8423
                     /gene="kdpE"
                     /locus_tag="OQL90_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS1369730.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8639..8896)
                     /locus_tag="OQL90_003041"
     CDS             complement(8639..8896)
                     /locus_tag="OQL90_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369731.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9019..>11187
                     /gene="speF"
                     /locus_tag="OQL90_003042"
     CDS             9019..>11187
                     /gene="speF"
                     /locus_tag="OQL90_003042"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCS1369732.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQ"
BASE COUNT         2502 a         2843 c         3065 g         2777 t
ORIGIN      
        1 aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatattt ttcctgaata
       61 tatttcagct gggtaatacc atatctaaat atttacctga acatcttcct aataaaaaaa
      121 ccgccagcat ctccactagc ggttttcaat gtattcaaaa ctcggcaatc agtaaaaatc
      181 accggttgcc ttttcggcct gttccatcca aaccggttta tcgctggttt tcgaccagac
      241 gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga aaggcagcgc
      301 cagaacgccc gctgcaaccg catatgtacg gcgtaaaaag ataagccatg caggatattc
      361 tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaata gcaccggaaa
      421 aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta ctactcatcc
      481 gaccacttat ttttgcttat tgatggttta tttacattca ctttgtaatt aagttgcaca
      541 aaagttaaat taatgctaaa cattagttaa accatggctt ttgccatttt tatacttttt
      601 tacaccccgc ccgcagattt ttgcgaaatc tttgcagcca gaattctact cttccggtat
      661 cacttttagg ccactggagg tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg
      721 tgtttttatt actgggttat ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc
      781 tgcgcaaggg ttcttactga ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc
      841 tttaggcagc gggctggcgc ggctgattaa tgacattcct cttccaggta cagcgggcgt
      901 tgaacgtata ctctttcgtt tacctggcgt ctctgaccat gagatgaact ggaagcaata
      961 tctttgtgcc attctcggcc tgaacatgct ggggctggcg gtgctgtttt ttatgttgct
     1021 cggtcagcac tatctgccgc ttaatccaca gcagttgccg gggctgtcgt gggatctggc
     1081 gctgaatacc gccgtcagct ttgtcacgaa taccaactgg caatcttata gcggtgaaac
     1141 cacgttgagc tatttcagcc agatggcagg cttaacggta caaaactttc tttctgccgc
     1201 cagcgggatt gcggtgattt ttgccttcat ccgtgcgttt actcgccaga gcatgagcac
     1261 actcgggaat gcctgggtcg atctgctacg catcacgtta tgggtgctag tccctgtggc
     1321 gttgttgatc gcactgtttt ttattcaaca aggtgcgctg caaaactttc tgccctatca
     1381 ggcagtgaat accgttgaag gagcgcaaca gctgttaccc atgggaccag tagcttctca
     1441 ggaagcgatc aagatgctcg gtactaacgg cggtggcttc tttaatgcca actcgtcgca
     1501 tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag atgctggcga tcttcttgat
     1561 cccaacagct ctgtgctttg cctttggtga agtgacgggc gatcgccgcc aggggcgcat
     1621 gttgctgtgg gcgatgtcag tgatttttgt catctgcgta ggcgtggtta tgtgggcaga
     1681 agttcagggt aatcctcatc tgctggcgct cagcgcagac agcagcatca atatggaagg
     1741 taaagagagc cgtttcggcg tgctggtcag tagcctgttt gcggtcgtga ctacggcggc
     1801 gtcctgtggc gcggtgattg cgatgcatga ttcgtttacc gctctcggcg gcatggtgcc
     1861 gatgtggcta atgcaaattg gtgaagtggt gttcggcggt gtcggttctg gtctttacgg
     1921 catgatgctg tttgtcctgc tggcggtgtt tattgccggg ctgatgattg gtcgtacacc
     1981 ggaatatctg ggtaaaaaaa tcgacgtacg cgagatgaaa ctgactgcac tggcgattct
     2041 ggttactcca acactggtac tgatgggcgc ggcgttggcg atgatgaccg acgccggacg
     2101 tagcgccatg cttaaccctg gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcgtc
     2161 cgccgctaac aacaacggca gcgcctttgc cggattaagc gccaactctc cgttctggaa
     2221 ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc
     2281 aattgccggt tcgctggtga gtaaaaagag ccaacccgcc agctccggca cgctgccaac
     2341 gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg gcgcactgac
     2401 ctttatccct gccctggcgc ttggtccggt ggcggaatat ctctcctgat gatattgagt
     2461 gagcactgaa tatgagtcgt aaacaactgg cgctattcga accaacactt gtcgttcagg
     2521 cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt
     2581 ttatcgtctg gatcggcagt ctgctgacca cctgtattag catcgcgatg gcaagcggtg
     2641 cgatgcctgg caatgcgctg tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac
     2701 tgttcgctaa tttcgccgag gcgctggcag aaggccgcag taaagcgcag gccaacagtc
     2761 ttaaaggggt gaaaaaaact gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg
     2821 cggcggacaa agttcctgcc gatcaactgc gtaaaggtga catcgtgctg gtagaagctg
     2881 gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg
     2941 ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca
     3001 ccggcggcac gcgtattctt tctgactggc tggtgattga gtgtagcgtt aaccccggtg
     3061 agacatttct ggatcggatg atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga
     3121 acgagattgc cctgactatc ctgctgattg ctctgaccat cgtcttttta ctggcaaccg
     3181 ccacgctgtg gccgttttcc gcgtggggcg gtaatgccgt gagtgtaacg gtactggtgg
     3241 cgctgctggt ctgtctgatc ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg
     3301 ccgggatgag tcggatgctg ggcgcgaatg tgattgccac cagcggacgt gcagttgaag
     3361 cggcaggtga cgttgacgtt ctgctactgg ataaaaccgg caccatcaca ctcggtaacc
     3421 gtcaggcgtc ggagtttatc cctgcgcagg gcgtggatga aaaaacgctg gctgacgccg
     3481 cacaactggc ttcgctggct gatgaaacgc cggaaggccg cagtattgtg atcctcgcca
     3541 agcaacgttt taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt
     3601 ttactgcgca aagccggatg agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt
     3661 ctgtcgatgc cattcgccgc catgttgagg ctaacggcgg tcacttccct gccgatgttg
     3721 atcaaaaagt cgatcaggtt gcgcgtcagg gggccacgcc gctggtggtg gtggaaggtt
     3781 cccgtgtgct gggcgttatt gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct
     3841 tcgcccagct gcgcaaaatg ggcattaaaa cggtgatgat taccggcgat aaccgtctga
     3901 ctgccgccgc cattgctgcg gaagcgggcg tcgatgattt tctcgccgaa gcgacaccgg
     3961 aggccaagct ggcattgatt cgtcagtatc aggcggaagg ccgtttggtg gcgatgaccg
     4021 gcgacggcac caacgatgct ccggcgctgg cgcaagcaga tatcgcggtg gcgatgaact
     4081 ccggcaccca ggcggcgaaa gaggcgggca atatggtcga tctcgactct aacccgacca
     4141 agttgatcga ggtggtgcat attggcaaac agatgctgat gacccgtggc tcgctgacca
     4201 ccttcagcat taccaacgat gtggcgaaat acttcgccat tattccggcg gcgttcgcgg
     4261 caacgtatcc gcagttaaat gcgctgaaca tcatgcgcct gcattcgccc gactccgcca
     4321 ttctcagtgc agtcattttc aacgccttga ttatcgtctt tttgattcct ctggcgttaa
     4381 aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac ttatggattt
     4441 acggtctggg tgggctgctg gtgccgttta ttggtatcaa agtcattgat ttactgctga
     4501 ccgtttgcgg tctggtgtga ggtttaccat gcgtggctta cgtccggcat tatcgacatt
     4561 tatctttctg ttattgatta ctggcggcgt ttacccgctg ctgaccaccg tgctggggca
     4621 atggtggttt ccctggcagg ccaacggttc gttgattcgt gaaggtgata cggtgcgggg
     4681 ttcggcatta atcgggcaga attttaccgg caacggcaga aatgccctat aatccacagg
     4741 cttctggcgg gagcaatctg gcggtcagta accctgagct ggataaacaa atagccgcac
     4801 gcgttgctgc attacgggcc gctaacccgg atgccagtac gaacgttccg gttgaactgg
     4861 tgacggcatc ggcaagcggg ctggacaata atatcacccc gcaagcggcg gcctggcaga
     4921 tcccacgcat cgcgaaagcg cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa
     4981 aatacaacca acaaccgctg gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac
     5041 tcaatctggc gctggataag cttgatgaat aacgaaccct tacgtcccga ccccgatcgt
     5101 ctgctggaac aaactgccgc gccgcatcgg gggaagctga aagttttctt cggtgcctgt
     5161 gcaggcgtcg ggaagacctg ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg
     5221 ctggatattg tggttggcgt ggtagaaacc cacgggcgaa aagataccga cgcaatgctg
     5281 gaagggctgg ctgttctgcc gccaaaacgc ctggcgtacc gtgggcggca catctgcgag
     5341 tttgacctcg atgccgccct cgcccgccgt ccggcgctga tcttaatgga cgaactggcg
     5401 cacagtaatg cgctaggttc tcgtcaccct aaacgctggc aggacatcga agagctgctg
     5461 gaagcaggca ttgatgtttt cactaccgtc aacgttcagc atctggaaag tctgaatgat
     5521 gtggtcagcg gcgtcaccgg aattcaggta cgggaaaccg tgcccgatcc ttttttcgat
     5581 gccgccgacg acgtggttct ggtggacttg cccccggacg atctgcgcca gcggctgaaa
     5641 gaaggcaaag tctatattgc cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa
     5701 ggcaatctga tcgccctgcg cgaactggca ctgcgccgta ctgccgatcg cgttgatgag
     5761 caaatgcgcg cctggcgggg gcatcctggc gaagagaaag tctggcacac gcgcgacgcg
     5821 atccttttat gcatcggcca taacaccggt agtgagaagc tggtccgcgc ggcggcgcgg
     5881 ctggcgtcac ggctgggtag cgtctggcac gcggtgtatg ttgaaacccc tgccctgcac
     5941 cgcttaccgg aaaaaaaacg tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg
     6001 ggcgcggaga cggcaacact ttctgatcca gcggaagaaa aagcagtagt gcgttatgcc
     6061 cgtgaacata atctcggcaa gattattctc ggtcgcccgg cctcgcgccg ctggtggcgt
     6121 cgggaaacgt ttgctgaccg actggcgcgc atcgcccccg atctcgatca ggtgctggtc
     6181 gcgcttgatg aaccacccgc ccgcacgatt aacaacgcgc cggatagccg ctcttttaaa
     6241 gacaagtggc gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc
     6301 actttaattg ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat
     6361 ctgcttggcg tggtggtggt ggcgctattt tatggacgct ggccttcggt ggttgctacc
     6421 gtcattaatg tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc
     6481 tctgatgtgc aatatctgct gaccttcgca gtgatgttaa ccgtcgggct ggtgatcggg
     6541 aaccttactg ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca
     6601 cggcacttat atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc
     6661 gccaccatcg aacaatttat tgcctccacg tttcatgccc gcagtcaggt gttgttgccc
     6721 gatgacaacg gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat
     6781 accatcgcgc agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta
     6841 cccggtgtac cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cgggctggtg
     6901 gtggtggagc cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag
     6961 acgtttacgc tgttagtcgc caatgccctt gagcggctga cgctaaccgc cagcgaagaa
     7021 caggcgcgga tggcaagcga acgtgaacag atccgcaacg ccctactggc agcgctttcg
     7081 catgatttac gcacgccgct cacggtgctg ttcggtcagg cagaaatctt aacgctcgat
     7141 ctggcaagcg aaggatcgcc ccacgcccgc caggccagcg agattcgtca gcatgtgctg
     7201 aacactaccc gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt
     7261 aatttgaaga aagagtggtt aacgctggaa gaagtggtcg gtagcgcgct gcaaatgctg
     7321 gaaccgggtt tatcgtcgcc catcaatctt tctctgccag aaccgctgac cttaatccac
     7381 gttgacgggc cactctttga acgggtgctg attaatctgc tggagaacgc ggtgaaatat
     7441 gcgggtgcgc aggccgaaat tggtatcgat gcccacgttg agggcgaaaa tctacaactg
     7501 gatgtctggg ataacggccc cggtcttccg ccaggccagg agcagacgat atttgataag
     7561 tttgctcgcg ggaataaaga gtcggcagta ccgggggtag ggcttggact ggcaatttgt
     7621 cgggcgatag tggatgtaca cgggggcact attaccgcgt tcaaccgacc ggaaggtggt
     7681 gcctgttttc gtgttacact tccccagcaa actgcccctg aacttgaaga atttcatgag
     7741 gatatgtgac aaacgttctg attgttgaag atgaacaggc tattcgtcgc tttctgcgca
     7801 cggcgctgga gggcgacggg atgcgcgtct atgaggccga aacgctgcaa cgcggcttgc
     7861 tggaagcggc tacccgtaag ccagatttga ttattctcga tctcggcctg cccgatggtg
     7921 atgggattga gtttatccgc gacctgcgcc agtggagcgc ggtgccggtg attgtgcttt
     7981 ccgcacgcag cgaagagagc gacaaaatcg ccgcgctgga tgccggagcg gatgattatc
     8041 tgagtaagcc gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc
     8101 actccgctac cgccgcgccc gatccgctgg tgaaattttc cgatgttacc gtcgatttag
     8161 ccgcccgcgt gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccacc
     8221 tgctggcggt gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc
     8281 aggtgtgggg gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc
     8341 tgcgacaaaa actggaacag gatcccgccc gcccacgcca tttcattact gaaaccggta
     8401 ttggctatcg gtttatgctt tgaatattaa ttttaataca gcctgccttt tattaattaa
     8461 agccgtaata ataaatacgg ctttttatct taaacaacac acaaaaataa caattcaata
     8521 ttttatatta ctgagtaaaa agctcatcat taaataaatt aagattgatc attttttatt
     8581 gatcaccttc acagttcaac cgtatttcct ggatagaata tcttcacctt cattcacatc
     8641 aggaaaggta aattaaatgg aaaataacag ccgcactatg ccccatataa ggcggacaac
     8701 tcatattatg aagtttgctc atcgcaatag cttcgacttt cacttcttca atgcccgtta
     8761 gtctaccgac taagggcact tcagcgtaca ggtcttcctg actctctgta ttacaggcat
     8821 atgcctgtat tccgcagtgc tcattctctg ttaacccttg tattatcagc caccggactg
     8881 gtaagggttt ctgcattctg aaaaaagcaa tctacctgat ttatttatca gcggggtaac
     8941 tttgcttttt tccggcacga tcgatttctc attcgagaaa ttgaggacct gctattacct
     9001 aaaataaaga gatgaaaaat gtcaaaatta aaaattgcgg ttagtgattc ttgcccggac
     9061 tgttttacca cgcagcgaga atgtatctac attaatgaaa gtcgtaatat cgatgtggcg
     9121 gcaatcgttt tatcgctcaa cgatgttaca tgcggaaaac tcgatgaaat cgatgccacg
     9181 ggttatggca tcccggtatt tattgctact gaaaatcaag aacgtgtacc cgcagagtat
     9241 ttgccctgta tttcgggtgt ctttgagaat tgcgaatcgc gacgagaatt ttatggtcgt
     9301 cagttagaaa ccgctgccag ccattatgaa actcaactgc gcccaccttt cttccgcgca
     9361 ctggtcgatt atgtcaatca aggtaacagc gcgtttgatt gccctggtca tcagggcggc
     9421 gaatttttcc gtcgccatcc ggcggggaat cagtttgtgg aatactttgg tgaggcgctg
     9481 ttccgttccg acttgtgcaa cgcagacgta gcaatgggtg atctgctgat tcacgaaggc
     9541 gcgccatgca ttgcacagca acatgcggca aaagtgttca atgccgataa aacatacttc
     9601 gttctaaatg gcacttcatc ttctaacaaa gtggttttaa acgccctgct gacaccgggt
     9661 gatctggtgc tgtttgaccg caataaccac aaatctaacc accacggagc gttgctacag
     9721 gctggtgcaa caccggttta tctggaaacc gcacgtaacc cgtatggctt tatcggtggc
     9781 attgatgcgc actgttttga agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg
     9841 cagcggacaa aagaggctcg tcctttccgc ctcgccgtta ttcagctagg cacctacgac
     9901 ggtacgattt ataacgcccg ccaggtggtg gataaaattg gtcatctgtg tgactacatt
     9961 ctgtttgact ccgcatgggt cggctatgag caatttatcc cgatgatggc tgactgttca
    10021 ccgctgttgc tggatcttaa tgagaacgat ccgggcattc tggttacgca atctgtgcat
    10081 aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag ccacatcaaa
    10141 gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat gcatgcctcc
    10201 accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat gcatgaaggt
    10261 gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc ccgcaagctg
    10321 atcctcgata actgtcagca tattcgtccg ttcgtacctg aactagtgga tggtaaaccc
    10381 tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca gtttgtacca
    10441 ggggaacact ggcactcttt tgaaggctat gcagagaatc aatactttgt cgatccatgc
    10501 aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga agcgttcggt
    10561 gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc
    10621 gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa acttcagcaa
    10681 cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa
    10741 gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac ccttcgtcag
    10801 ttatgccagg aaatgcatga tttgtatgcc cgtcacaacg tgaaacaacc gcaaaaagag
    10861 atgttccgta aggaacactt cccacgcgta agcatgaatc cacaagaagc caactacgcc
    10921 tattcacgcg gtgaagtgga gctggttcgt ctgccggatg ccgaaggccg tatcgctgcc
    10981 gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt
    11041 ggtgctgttt tgcgttactt cagcgctctg gaagaaggga tcaacctgct gccaggtttt
    11101 gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc
    11161 tatgtcatca agcctcgtga tgcgcaa
//
LOCUS       DAJVOY010000133        10996 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVOY010000133 DAJVOY010000000
VERSION     DAJVOY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.133"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..371)
                     /gene="yehX"
                     /locus_tag="OQL90_003043"
     CDS             complement(<1..371)
                     /gene="yehX"
                     /locus_tag="OQL90_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1369733.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGL"
     gene            complement(364..1521)
                     /gene="yehY"
                     /locus_tag="OQL90_003044"
     CDS             complement(364..1521)
                     /gene="yehY"
                     /locus_tag="OQL90_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1369734.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(1528..2445)
                     /gene="osmF"
                     /locus_tag="OQL90_003045"
     CDS             complement(1528..2445)
                     /gene="osmF"
                     /locus_tag="OQL90_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1369735.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(2697..4994)
                     /gene="bglX"
                     /locus_tag="OQL90_003046"
     CDS             complement(2697..4994)
                     /gene="bglX"
                     /locus_tag="OQL90_003046"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1369736.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGSKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            5190..6905
                     /gene="dld"
                     /locus_tag="OQL90_003047"
     CDS             5190..6905
                     /gene="dld"
                     /locus_tag="OQL90_003047"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1369737.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(6944..7876)
                     /gene="pbpG"
                     /locus_tag="OQL90_003048"
     CDS             complement(6944..7876)
                     /gene="pbpG"
                     /locus_tag="OQL90_003048"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1369738.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(8050..8637)
                     /locus_tag="OQL90_003049"
     CDS             complement(8050..8637)
                     /locus_tag="OQL90_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1369739.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            8807..9385
                     /locus_tag="OQL90_003050"
     CDS             8807..9385
                     /locus_tag="OQL90_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1369740.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(9515..10276)
                     /locus_tag="OQL90_003051"
     CDS             complement(9515..10276)
                     /locus_tag="OQL90_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1369741.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(10329..>10996)
                     /locus_tag="OQL90_003052"
     CDS             complement(10329..>10996)
                     /locus_tag="OQL90_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCS1369742.1"
                     /translation="EARLSALTNNQTKSLKLKPVALPKVASQLPDELGYSLLARRADL
                     QAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLP
                     IFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIER
                     DTLRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGG
                     YKR"
BASE COUNT         2603 a         3048 c         2788 g         2557 t
ORIGIN      
        1 tccagcccca gtagcgccat taattcgtcg atacgatcgt caatccgcgc ccgcgaccat
       61 ttttgtaatt gcggcacggt agcgatgttt tgcgccacgc tccagtgggg gaacaggcca
      121 atagattgaa tagcatagcc catccggcgg cgcaactcca gtactggcag cgagcgaatt
      181 tcttctccgg caaagcggat cactccgctg tcatgctcca ccaggcggtt aatcattttc
      241 agggtggtgg atttgccgga gccagatgtg ccaatcagca ccgaaaaact cccttcctga
      301 aaattgagat tgagatcgtt aacggctttt tgtgcgccga acagtttgct gacatggcta
      361 aattcaatca ttacgtttca ccttcagcag tgcgataagc aaatcgaaca gcgcgtcggt
      421 aagcaccgcc agaacaatta ccgggatcac ccccagcaac actaaatcaa tggcgctgct
      481 tagcagcccc tggaaaacca gcgcaccaaa accgcctgcg ccgattaacg ccgcaatcac
      541 cgccatacct acagtttgca ccatcaccac ccgcaggctg cgcagaaata ccggtaacgc
      601 cagtggtaac tgaacatgca ggaatcgctg cgccccgctc atgcccatcg ctctggcact
      661 ctccagcaca tcgcgcggga tctggttcaa accgactacc acgccgcgca ccagcggcag
      721 caaggcatag agcaccagcg caatcagtgc gggtgtcatt ccggttcctg ctatgccgag
      781 cttccccagc cacggaaagg ccgtcaccag cgcggcaagc ggcgcaatca acaggccaaa
      841 gagcgccacc gaaggcacgg tctgaatgac attgagcaga gaaaaaattg ccccctgccg
      901 agcagtggaa aagtagcacc agatgcccaa cggcacacca atcactaacg caggcagcac
      961 cgcaccaaac agcaacgtca gatgttgtgc cagcgcgtcg tcaaacacat cctgacggtt
     1021 ggcgtattct ttcattagtg agagatcgtt aagcgtgccg gagtacagca accacagcgg
     1081 aataatggca atctgcatat gcaacaacca gcgccacagc ggatgcgtgg agattcggcg
     1141 gatggcatcg ctacaggcca gcaatgccag cgccgcagcc agccagaaac cactgccgag
     1201 gctggtacgc gccagcgcac tgccattttg cgccagttgt gtcgccgcct ttcccgcgcc
     1261 ccacaccagc aatacgaaga cgaattgcgc cagaatgagt gcacaaatgc tgcctttttt
     1321 accgggaata aaacaggccg tcagccaggc gcaaccaacg cccaccagca tccagatcgt
     1381 ttgcggccac agctgccaga gatgacgccc ctcacccgaa actaaacgat taggcgcgta
     1441 actgataaac ggcagcgccg ctgcgattgc cgtcagcaac agcagcagcg ccagaacagg
     1501 attaatacgg aaataagtca cgggaaatta cttcgtccac cctttttgtt tcaggtagtc
     1561 ggcagccact tttttggcat ccagtccttc cacagcaatg ctggcattca gttgctgcaa
     1621 tgtttttgca tcgaggctgg cgaagactgg ctgtagccac tgtgccattt gcggatactc
     1681 cttcaacacc gactcacgca ccactggtgc aggcgcgtag ataggttgca caccttgcgg
     1741 atcgcttaag gtttgcagcc ccagcgccgc taccgggccg tcagtgccgt aagccattgc
     1801 agcattaacg ccagagattt gctgggcagc ggctttgatc gtcaccgccg tgtcgccacc
     1861 agccagtgac agcaactgat cctgaccgag cttaaagcca taggcttttt caaacgcggg
     1921 taacgcatcg gcgcgttcga taaactctgc cgaggctgcc agtttgaagg tgccgccctc
     1981 tttcagataa cgactcaggt cggcaagcga agtgagttta tttttctctg ccacatcctg
     2041 acgcacggcg atagtccagg tgttatttgc aggcgcgggc gtcagccaga ttaacttgtt
     2101 gtgctccgaa tcgagctttt tgactttctc gtaaccttgc tgcgcgtttt tccacgctgc
     2161 atcgttttca tctttaaaga agaaagcgcc attgccggta tattccggat agatatccag
     2221 ttcaccggaa gtaatcgccc cgcgcaccac aggagtcgtt ccgagttgca ctttattcac
     2281 cgtcggtacg ccgtggcttt ccagcacctg caagatgatg ttgccgagta gcgcgccttc
     2341 ggtatcgatt tttgaaccga ctttaacggg ggaagccgcc tgtagcggca ggctcacggc
     2401 tgccaacata accagtgaac ctgcccagac ctttgagagt ggcatgatgc tttcctcatt
     2461 cttttactgt tgttttcagc gaattaagag aaaagcatag ttgataatga cagagttagc
     2521 ctgagcggaa ggattcagtt gcagaatcag ataaatactt aaagggcata ttttgcccgg
     2581 cgtttcttgc cggatgcggc gtaaacgcct tatccggcct acaaaggtcg aaaattcagc
     2641 aagttaagat atgaacatca ggcaaagggc gcaaacgtcg cgcccttact aaagcattac
     2701 agcaactcaa actcgccttt cttaacgcgt gcggaatcag tgccgataaa gacattgaac
     2761 ttgccaggct cggcgtcata tttcatctgt tgattccaga acttcagcgc ctcgatatca
     2821 atcgggaagc tgacagtctg agtttcgccc ggtttcaggg tgattttctc aaagcctttc
     2881 agctgcttca ctggacggct catggaagcc gtcacatcct gcaagtacat ctgtactacc
     2941 gtggcccctt cgcgtttacc ggtgttcgtc acctgcacgc tggccgtcac tttgccgtca
     3001 cgcttcatgg tcggcgcaga aagtttcaca tcagagacgg tgaaagtggt atagctcaga
     3061 ccatagccga acggataaag cgccccgtta gcttcatcaa aataacgcga agtgtatttg
     3121 ttcggcttgt cggcattata cggacgaccg gtgttcagat ggctgtagta caccgggatc
     3181 tgcccgacag aacgcgggaa ggacatcggc agcttgccgg acgggttgta atcgccaaac
     3241 aacacatcgg caattgcatt accgccttca gtccccgcaa accaggtttc cagaatcgca
     3301 tcagcctgct gatcttcttt caccagcgcc agcggacgcc cgttcatcag caccagcacc
     3361 agcggtttac cggtggcttt cagcgccgca atcaagtcac gttggctttg cggaatagtg
     3421 atatcggtac ggctggaggc ctcgtgcgcc atcccctgtg cttcaccgac cacagccacc
     3481 accatatcag attgtttcgc cgtctgcacc gcttcatcaa tcatctcttg cggcgagcgc
     3541 ggatcgactt tgaccgcttc ttcatactga ttcaggaaat cgataatgcc tttgtcactg
     3601 gtaacgttcg cccctttggc atacagcact ttaccgtttt caccgacggc atttttaatc
     3661 ccggtcagta cggtcacgga ttgatcggca acaccggctg cggaccagct gcccatcacg
     3721 tcgcgtttac tgtccgccag tggcccaacc accgcaatgg tggccgattt tttcagcggt
     3781 aacgtttcga gacggttttt cagcaacacc aggctttcgc gcgccacttc acgtgcttct
     3841 ttacggtgca ggcggctttc ggcattggta tccaccgggt cagactcttt cgaccccaaa
     3901 tggctgtatg ggtcgttaaa caatcccata tcatatttaa cgttcagtac atggcgggca
     3961 gcatcgtcca gctcttccat cgtcactttg ccggatttga tcaacccagg cagatacttc
     4021 gagtaatact cgtcgctcat actcatgttg attccggatt tcagcgccac gcgcaccgca
     4081 tcttccgggt ctgccgccgt gccatgttta atcagctctt tgattgcacc gtgatcggaa
     4141 acggtgatgc ctttaaagcc ccactggtcg cgcagaacat ctttcagcag ccaggaatcg
     4201 gaggtggctg gcgtgccgtt cagcgagttc agcgccacca tcaccgcgcc gctgcctgcg
     4261 tccagccccg ctttgtacgg cggcatataa tcattaaaca ggcgctgcgg actcatatcg
     4321 acggtgttgt actctttacc gccttctacc gcgccgtatg cggcaaagtg tttgacgctg
     4381 gtcatcaccg agtagcgatc tgccgggctt ttaccctgca tcgcttccac catggtttta
     4441 cccattgttg aggtgagata cgtatcttcg ccaaaacctt cggaagcacg tccccagcgc
     4501 ggatcgcgcg agacatcgac catcggtgcc caggtcatat tcaggccatc atctgccgct
     4561 tcataagcag agacacgtcc gaccgttttc actgcatcga ggttaaaaga cgaggccaga
     4621 ccgaggctaa tcgggaacac cgtgcgctga ccgtgcagca cgtcgtaagc aaagaaaaga
     4681 ggaattttca ggcggcttaa ttccatcacc tgatcctgca tggcgcggat atcctgacgg
     4741 gttacggtgt tgaaaatcgc cccaacctga ccgtctttga tcatctcgcg gatcgcctct
     4801 ttcgggttat ccgggccgac gctgattaag cgcagctgac caattttctc atcaactgtc
     4861 attttcttaa gcaattcggt gacgaacgca tcccgcgctt cgggcgtgaa tggatggttg
     4921 ccgaataaat catccgccag tgctggctgc agggccagac tcaccgcgat tcctactgaa
     4981 catagccatt tcatatggat ttttcttcct ctgttgcccg acgaggcagc gaaaatggtg
     5041 caaaaaccgt agtttgccat aagcatgatg gagagagaaa aagaatgctc agtttattgt
     5101 ctgaattttc aaaatattca ctcgctgaat tgtcatacaa ggcgctattc tagtttgtga
     5161 tattttttcg ccaccacaag gagtggaaaa tgtcttccat gacaacaact gataataaag
     5221 cctttttgaa tgaacttgcc cgtctggtcg gtcattcaca cctgctcacc gatcccgcaa
     5281 aaacggcccg ctatcgcaag ggcttccgtt ctggtcaggg cgacgcgctg gctgtcgttt
     5341 tccctagctc actactggaa ttgtggcggg tgctgaaagc ctgtgtcacc gccgacaaaa
     5401 ttattctgat gcaggctgcc aatacaggcc tgaccgaagg atcgacgcca aacggtaacg
     5461 actatgatcg cgatatcgtg atcatcagca ccctgcgtct cgacaagctg cacgttcttg
     5521 gcaagggcga acaggtgctg gcctatccgg gcaccacgct ctattcgctg gaaaaagccc
     5581 tcaaaccgct gggacgcgaa ccgcactcag tgattggatc atcgtgtata ggcgcatcgg
     5641 tcatcggcgg tatttgtaac aactccggcg gctcactggt gcaacgtggc ccggcgtata
     5701 ccgaaatgtc gttattcgcg cgtataaatg aagacggcaa actgacgctg gtgaaccatc
     5761 tggggattga tctgggcgaa acgccggagc agatccttag caagctggat gatgatcgca
     5821 tcaaagatga cgatgtgcgt cacgatggtc gtcacgccca cgattatgac tatgtccacc
     5881 gcgttcgtga tattgaagcc gacacgcccg cacgttataa cgccgatcct gatcggttat
     5941 ttgaatcttc tggttgcgcc gggaagctgg cggtctttgc agtacgtcta gataccttcg
     6001 aagcggaaaa aaatcagcag gtgttttata tcggcaccaa ccagccggaa gtgctgaccg
     6061 aaatccgccg tcatattctg gctaacttcg aaaatctgcc ggttgccggg gaatatatgc
     6121 accgggatat ctacgatatt gcggaaaaat acggcaaaga caccttcctg atgattgata
     6181 agttaggcac cgacaagatg ccgttcttct ttaatctcaa gggacgcacc gatgcgatgc
     6241 tggagaaagt gaaattcttc cgtccgcatt ttactgaccg tgcgatgcaa aaattcggtc
     6301 acctgttccc cagccattta ccgccgcgca tgaaaaactg gcgcgataaa tacgagcatc
     6361 atctgctgtt aaaaatggcg ggcgatggcg tcggtgaagc caaatcgtgg ctggtagatt
     6421 atttcaaaca ggccgaaggc gatttctttg tctgtacgcc ggaggaaggc agcaaagcgt
     6481 ttttacaccg tttcgccgct gcgggcgcag caattcgtta tcaggcggtg cattccgatg
     6541 aagtcgaaga cattctggcg ttggatatcg ctctgcagcg taacgacacc gagtggtatg
     6601 agcatttacc gccggagatc gacagccagc tggtgcacaa gctctattac ggccatttta
     6661 tgtgctatgt cttccatcag gattacatag tgaaaaaagg cgtggatgtg catgcgttga
     6721 aagaacagat gctggaactg ctacagcagc gtggcgcgca ataccctgcc gagcataacg
     6781 tcggtcattt gtataaagca ccggagacgt tgcagaagtt ttatcgcgag aacgatccga
     6841 ccaacagtat gaatccgggg atcggtaaaa ccagtaagcg gaaaaactgg caggaagtgg
     6901 agtaaaaatt acggatggca tagtatcgcc atccgtcatt cacttaatcg ttctgtgccg
     6961 tctgccccgc cgccgccatt tgggcggctt tttgtttttt atagctcaac gctgctgctg
     7021 gaacaggcat cactttaccg gtttcaatcc aggtacgcag gcggctggca tcggcaaaat
     7081 gggtatattt gccaaacgca tccatcacta ccagcgccac cggtttatta ttgataaccg
     7141 tacgcatcac cagacaatgg cccgccgcat tggtaaaacc ggttttggtt aactgaatat
     7201 tccagttatc gcgatacacc agatggttag tattgcggaa cggcagcgta tacgtcgggt
     7261 tagagaaggt tgccatatct tcccgagtag tacttaactg cccgatcaac ggatattgtt
     7321 tgctggcaat gagcagtttg gttaagtcac gggcagttga aacgttatgc accgacaatc
     7381 cggtaggttc aacaaagcgc gtgttgttca ttccgagcga tttcgctttc gcattcattg
     7441 ccttaataaa ggctttgtaa ccaccgggat aatggtgtgc aaggcttgcc gccgcgcggt
     7501 tttctgaaga catcagcgcc agcaacagca tatctttacg gctgatttcg ctattcagtc
     7561 gtacgcgcga atagacccct ttcatctccg gcgtctggct gatatccact tttagttttt
     7621 catccagcgg cagtcgtgca tccagcacaa ccatcgcggt cattaatttg ctgatagacg
     7681 caatcggacg caccagatcc gggtggttcg agtagatcac tttgttggta ttcagatcaa
     7741 caatcatcgc gctaccggag gcaatttcag gttgtgaagc ggtgatagcg gctgccgttt
     7801 tcgcaacggc ctgcggtgcc aaaggcacag ccagcatcag ggccaggcta aataaagaaa
     7861 ctcgaaattt cggcatgatg agcattcaga tagtggttca cgcgcacagg ttgcgcaccg
     7921 ccggagtaag gatttactga ggctagcgac gccatcataa cgagcaaaaa gtgcgatcgt
     7981 caaaggagaa tcgtgaggaa atgctgcatt gctgacattt acgccagcaa tgcaacgtca
     8041 aagaactttc tagaacaaac gataaccgta gccccatagt ataacggtta gggcgagcag
     8101 cacttccagt accagcacgc caatcgccag cgtcgaacta gagaagctaa ggccttcctc
     8161 tttgttgata ttcaggaagc tcggaatacc aaggtaaagc aggtagccgg tgtaaaacag
     8221 cgccaccgtg ccgaccagcg cacacaacca gaccagtgga taaagcgcca ccaaaccgct
     8281 taaaaacagc ggagttgcaa cgtagcccgc gaagaccatg cagtgcgcaa gtgacggacg
     8341 ctgcggataa ttacgcgcca tccaccagat gacccgcccc attaccgcaa ccccagccag
     8401 cataacgcca taaaacagga cagccagcgc cagtccggta aaccaggata acttcaggat
     8461 agtgccatcg ccaaaattcc agccaatctg tgtagtgcca atgaaggcgc aaatcaccgg
     8521 aatcgccgcc atcagcaaaa cgtggtgggt gtaatggtga gaaatcgttt cgttttcgcg
     8581 attaatcacc tgcatttcac gatcgggatg ggaaaacagt ccccagacat ggctcatacc
     8641 gcccccttgt tgtgagttca tgaacctgac agttcaagta taagtcagct tgtgattatt
     8701 ttttgttcgc tcatgtaatt tgcaccgtct gatgtacgtc ctttttcagc gtgaatgatt
     8761 tccagggtgt atgcttacag gcaaccaaag ggagacagac tggcctatgg atctcaatac
     8821 actcatctca caatatggtt atgccgcgct ggtgatcggt agcctggcgg aaggtgaaac
     8881 cgtgactttg ctgggaggcg ttgcggcaca tcaggggcta ttaaagttcc cgctggtggt
     8941 actttctgtg gcgcttggcg gcatgattgg cgaccaggtg ctttatctgt gcgggcggcg
     9001 gtttggcggc aagctgttac gccgtttctc gaaacatcag gataaaattg agcgggcgca
     9061 gaaacttatc caacgccatc cgtatctgtt tgtcattggt acgcgcttta tgtatggctt
     9121 tcgggtgatt ggcccgacgc tgattggtgc cagccagctg ccgccgaaaa tctttctgcc
     9181 gctgaatatt ctcggcgcat ttgcctgggc gttgattttt accactattg gttacgctgg
     9241 tggtcaggtg attgcgccgt ggttgcacaa tctcgaccag catttgaagc actgggtctg
     9301 gttgattctg gtcgtggttc tggtggtggg cgtgcgctgg tggctgaaac ggcgcgggaa
     9361 gaaaaagccg gatcatcagg cgtaaaacca ttgccctggg taaggcgttc gcgccgcatc
     9421 cgacatccgg tgcaccatgc ctgatgcgac gcttgtgcgt cttatcatgc ctaccaaaat
     9481 tttacccatt gaaataacca cgcacctcac tgcgctattc tgggttgaac tgcggattcg
     9541 ccaacataaa gccgccatcc actatcaacg actgcccggt ggtgtaattt gcgccctctg
     9601 agcacagcca cgctaccagg ctggcaatct catgcgttgc gccaaaacgc cgcagaggaa
     9661 tcgaaggctc cgcgtcgggc ttcacgtcgc tgtcatccat gccattcatt ggcgtggcga
     9721 tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct gaccagctcc agcgccatcg
     9781 ctttggttaa cccaccgagc gcatgtttag cggctgtgta ggcgctggca tccggcagcg
     9841 gcgtatgttc atgtaccgat gtaatgttga tgatgcgacc gccctgccct tgtttcacca
     9901 tctggcgggc cgcaatttgt gagcataaaa atgcaccatc gacatcaacg gtaaaaatct
     9961 tgcgccactc atcgaaagcc atatcaagaa acggcgcttt ggtcattgcg cccgcattat
    10021 tcaccagcac atcaatacgc cccagccgtt gaatgagttt ctccagcgcc tgcgcacctt
    10081 ctggcagttt accgagatcc agctgcacga tctccgcacg tacgccgtgg ctaactacct
    10141 cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca ggtaatgcca atatcaaacc
    10201 cttgctgcgc cagtaataac gcgcactctt tgccgatccc cgaatcggag gcggtaataa
    10261 tcgcaacctg tgccatcgag ttctccactt aacgctgaat aaacgttaag tatagaaggc
    10321 gcatatcatc agcgtttgta acccccgccc aacgcaccag tgagttgaat ggaggcatcc
    10381 agccactgcc cttgcaataa caggccattg gcccgctcac gcagcgcggg gattctggct
    10441 tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc gcgcctgcgc aagacctacc
    10501 acacgcaagg tatcgcgctc aatttgcgcc tgatgctggt ttttctccgc aagtgtctga
    10561 acctgactgg ctgcccgcgc cacgtcattc accgcttcaa ccaccgcttt gttgtagctg
    10621 gcgatagaca agttgctttc ggcttttgcg atatcgagat tggcgttaag acgaccacta
    10681 tcgaaaatgg gtagcgtcag gcctgccgta acgcccattt gctgcgcgga atgacggaac
    10741 agatcgctta agtgcaacgc atcctgttgc aggaaggcca tcaggttgat gtcaggataa
    10801 aatgccgctt ttgccgcatc aatggtgctt agcgatgact caacgtacca gtgcgccgcc
    10861 tgcaaatctg cccgccgggc cagtaaggag taccccagtt catcaggaag ctggcttgcc
    10921 actttcggca acgcgaccgg tttaagcttc aatgactttg tctggttatt tgtaagtgcg
    10981 cttaaccgtg cctcaa
//
LOCUS       DAJVOY010000134        10900 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVOY010000134 DAJVOY010000000
VERSION     DAJVOY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.134"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..556)
                     /locus_tag="OQL90_003053"
                     /pseudo
     CDS             complement(1..556)
                     /locus_tag="OQL90_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(531..1331)
                     /gene="otsB"
                     /locus_tag="OQL90_003054"
                     /pseudo
     CDS             complement(531..1331)
                     /gene="otsB"
                     /locus_tag="OQL90_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1498..2484)
                     /gene="araH"
                     /locus_tag="OQL90_003055"
     CDS             complement(1498..2484)
                     /gene="araH"
                     /locus_tag="OQL90_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1369743.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2499..4013)
                     /gene="araG"
                     /locus_tag="OQL90_003056"
     CDS             complement(2499..4013)
                     /gene="araG"
                     /locus_tag="OQL90_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1369744.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGAIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4083..5071)
                     /gene="araF"
                     /locus_tag="OQL90_003057"
                     /pseudo
     CDS             complement(4083..5071)
                     /gene="araF"
                     /locus_tag="OQL90_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5785..6288
                     /locus_tag="OQL90_003058"
     CDS             5785..6288
                     /locus_tag="OQL90_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1369745.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6367..6618)
                     /locus_tag="OQL90_003059"
     CDS             complement(6367..6618)
                     /locus_tag="OQL90_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1369746.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6733..6819)
                     /gene="azuC"
                     /locus_tag="OQL90_003060"
     CDS             complement(6733..6819)
                     /gene="azuC"
                     /locus_tag="OQL90_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1369747.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7082..7405
                     /locus_tag="OQL90_003061"
     CDS             7082..7405
                     /locus_tag="OQL90_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1369748.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7576..8073
                     /gene="ftnA"
                     /locus_tag="OQL90_003062"
     CDS             7576..8073
                     /gene="ftnA"
                     /locus_tag="OQL90_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1369749.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8111..8350)
                     /locus_tag="OQL90_003063"
     CDS             complement(8111..8350)
                     /locus_tag="OQL90_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1369750.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8541..9752
                     /gene="tyrP"
                     /locus_tag="OQL90_003064"
     CDS             8541..9752
                     /gene="tyrP"
                     /locus_tag="OQL90_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1369751.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(9814..10479)
                     /gene="yecA"
                     /locus_tag="OQL90_003065"
     CDS             complement(9814..10479)
                     /gene="yecA"
                     /locus_tag="OQL90_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1369752.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10675..10761)
                     /locus_tag="OQL90_003066"
     tRNA            complement(10675..10761)
                     /locus_tag="OQL90_003066"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10725..10727),aa:Leu,seq:taa)
     gene            complement(10774..10847)
                     /locus_tag="OQL90_003067"
     tRNA            complement(10774..10847)
                     /locus_tag="OQL90_003067"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10813..10815),aa:Cys,seq:gca)
BASE COUNT         2738 a         2776 c         2513 g         2873 t
ORIGIN      
        1 ttttctgtct ggaaacccag caaatcataa tcacaaagct gttcaagcaa ggtgtcatat
       61 gtcggcagcg cgttgaagat ttccggtgtc gggaaaggaa tatgcagaaa gaaaccaatg
      121 cgattattca ccccacgttt gcgtaattca tgcgcaaagg gcaacaggtg ataatcgtgg
      181 atccagataa tgtcatcgtc ttgcaacagc ggcagtaatt tatctgccag caacgcattt
      241 acgcgtagat agccgtccca ggcaggacgc tgaaattgca tcagatcgag ccgataatga
      301 aaagcgggcc agagaacggc attggagaat tggttgtagt attcgtcaag gtcctgttcg
      361 ctgaggttaa aagaggccca cgtaatgtta ccttttttag cggctgatcc tcattccctg
      421 tttcaccact ccagccaaac cacagtccgc ctgtggcttt cagtgccccc agtatgccaa
      481 cggcaaggcc accggcactg gcggcgtgct cgtctggtgg tgcaatccgg ttagatacta
      541 cgactaaacg actcatagtc atcactcctg ttattttctc ttttttgttg taatgcggtg
      601 gttatcattt caagcctgct ccagacatcc ggcacacccg ccagtcgcca tgatgcctga
      661 gttgcacctg tgccaatttt tactgacatt ccgcccagtc ggttaacgac tgcgaagcca
      721 gattcatcgg ttaaatcatc gcccagaaat acgggcgttc gcccgataaa gggagcttcc
      781 tgcataaaag ctgcaattgc ctcaccttta ctggtacctc tcggtttgat ctcgacaaca
      841 cactttccct gctgtaacgc catttgtggc cagatctgag taatacgtta cgctaatgtc
      901 attaatgcgt cttcatgctg cggagcctga cgataatgca gcgcaaaagc catccctttc
      961 gcctccagct ccgcgccggg atactgagcg ataactgtat gcagttgcac gctaatatca
     1021 cgcgcaatcg catccggcag atgaacgata tgcgttttac cattgatgtc acggcgctcc
     1081 gccccatgca cgcccgctaa cgggaagcga taaggttttg ccagtgcgtc aagctccacc
     1141 attgagcgcc ctgatatcaa tgccaatgca ccatcacttg cggttgccag tagctgtagt
     1201 ccttgcagaa tattgtcagg cacgacgacc tgatcgggat gcggtttgat ttccgccagc
     1261 gttccatcga gatcaaaaaa ccaggcatat ttcgcggata gttcaggggt ttcggttaac
     1321 ggttctgtca cccggttctc ctacttctct tttcttattg ttttgcgcag aacgcgcttt
     1381 acagacatgt aagtatagac agtgtgacgg gggtcgccat ttgcaacgaa gaacagccaa
     1441 ccgttatggc ggttggctgt ggtgggaaaa aacgctaaat tgttgcagaa aaaagcatca
     1501 gacagtgcgt ttcgcttttt gcttgtaacg gtcgaagatc accgctgcca gcaggattaa
     1561 gccgcgaacc acgtactgcg cgaaaggaga aatattaagc aggttcatgg cgttttccac
     1621 ggtgcctaaa attaagatac ccgccaccac atatgagatt tttccgatgc ctcctttcag
     1681 agaaacgcca cctaaaacac aggcggagat aacaatcagc tcgtaaccaa tcgacgtcat
     1741 tggctgccca ctggtcatac gtgaagccag aataattccg gctatcgcag ataccaggcc
     1801 agagagaaca aagataataa ttttggtgcg aacaaccggt acacccgcca gacgcgcagc
     1861 ctcttcgttc ccgccaatcg ccagggtgtt acgaccaaag gtggttttat tcagcaacaa
     1921 accaaagata atcagacacg cgacggtgag ccagattggc gcaggcagac cgaaccagtt
     1981 agcgtaacca agggcaaaga agctttcatc ttcgataccg accgctttac cgtctgaaat
     2041 gatgtacgcc agacctcgaa caatctgcat cgttgccagt gttgtgatca gagcatttat
     2101 tttcagtttg gcgataacaa agccattgac caggccacag agaatgccaa gcagcaaccc
     2161 cgctgccacg ccaatccaca ggctttcagt caggttgata accaccgccg tggtgacacc
     2221 cgcacaggca attacggagg cgacagaaag gtcaaagtca ccggaagcaa ggcagaacaa
     2281 catgccacac gccaccatcc ccgacatgga aattgccagg cccaaccctt tcatattaat
     2341 gaaggtggca aaatttggga caaaaatggc acaggcgata aagagcaccg caaacaccac
     2401 cagcatgccg tactgatccc agatacgccc gaagctgaat gacgacttag gtgcgccaga
     2461 acccgatgta gaaacagaag acatcatact ctccttactc aggcaactgc ctggctgact
     2521 ttaggcatcg caaggctcag tgcctgacgc tcatctgcct gctcgtgtaa caattcaccg
     2581 gcgattgcac cttcccgcat caccacaatc cggtcggcaa cgccgaggac ttcaggtaag
     2641 tcgctggagg caaacagcac cgccacaccc tgcgccgcca gcgcataaat tacgttatat
     2701 atttcgtgct tagcaccaac atcaatgccg cgcgtaggtt catccagcaa aatgaccttc
     2761 atctcttccg ataaccagcg gcccagaatg gctttttgct gatttccgcc tgagagattc
     2821 atgatcagtt gctcagcgcc cggcgttttg atgttgagcg aacgaatgtg ctgatcggca
     2881 ttgttttctt cccaaccgtt gttgattaca caaccgccga gcacatgttt gcgtctggca
     2941 ctgatgttga tattgtcgcg aacggagtgc acgggaataa tgccttccgc tttgcgatct
     3001 tccgggcaga gcatcatgcc tgcggcaatg gcgtggctcg gtttacgaat atcgatcggc
     3061 tgttggtcga tataaacctg accggcggtg atttgcgtcc cgccaaacaa gcctttcatt
     3121 aattcgctac gccccgcccc taccagacca aacagcccaa caatttcacc actgcgaacc
     3181 gccagactta ttggcgtacg cacgcctggt gctttcacag catcaagacg caggcgctcc
     3241 tcgccataac tacgaggttg ccagccgtag atatcgccaa tgtcgcgccc gaccatcgcc
     3301 tgcaccagcg cgtcgtggtc aacctgctgc atatcggtaa aggttttgac ataacgtcca
     3361 tctttaaaga cagtgatggc atcgctgagg gcaaatattt cttccatacg gtgagaaacg
     3421 tataagatca cccgcccctc ttttcgcagt tcacgaataa cgcggaaaag attgtcgatt
     3481 tcgcgggcag aaagggagct ggttggctca tcaaaggcga taattttagc gttacgcgcc
     3541 agcgccttgg cgatttcaac catctgccac tgaccaatgg agagatattt cagcggcgtg
     3601 tccgggtcaa tatccatacc aagatgttta agttgtaaac ccgcctcata attcagcaat
     3661 gagcgattca caatgccgcc tttatgcggc agctggccga gatagatgtt ttccgcgacg
     3721 gtcatttccg gcacgagatg cagttcctgg taaataatcg ccacacccgc gttaagtgct
     3781 gcggtcgtgt cggaaaagga catttcctgc ccattaatca ctacagaacc cgtggttggc
     3841 gcatagttgc cgctgaggat ttttaagaga gttgattttc ctgcgccatt ttcacccatc
     3901 aacgcatgaa cctgaccggc atagcagtca aaactaatat ccgtcagcgc cttaacgccg
     3961 ggaaacgttt taccgatgcc gcgaaatgag agatacgggg tagactgttg cataacgtct
     4021 ccgtgaatca ctggtcgttc acaccccctc tgcatcatgc agaggggaat ttttccggca
     4081 aattacttac cgcctaaacc ttttttctcc agttcttctt taaagttgtc acgcgtgatc
     4141 agcaccacgt cggtaacttc ggtaaatttt ggcggttcaa cgtcttttgc tacccagttg
     4201 taaagcattt cgctggattt atagccatgt acgtccgggc ttggcagcag ggaaccgtag
     4261 aagccggttg cctgtacttt agacagttcg ctcaccgcat ccacaccgtt aatgccaatg
     4321 ccgataatat cggcagcttt aaagccctga ccttccgtcg cgcgtacgcc gcccagcacg
     4381 gtgctgtcgt tcataccgac gatcagccaa tgtttaactt caggatgttg aaccagcatt
     4441 gagttggcag cgtcaaatgc ccccgggatg tcgttagatt tggtaggtac ctgataaatt
     4501 tgtttttccg ggaatccggc cgctttcagc gcatccatag atcccgtagt acggcggcgg
     4561 gcagtatcca gttcgttggc ggtaatcacc atcaccgcgc tttctttgac atcccagcca
     4621 cgtttctgca tctctttata cagttcctga ccctgacgtt cgccaatttt agtcgcgcca
     4681 tcatcaccag cggaacggta tccattggct tacctttggc gttaacaaac tgatcatcca
     4741 cggcaatgac tttcatatcg tagccacgcg ctttcgcgac gatggcagag ccgagtttgg
     4801 ggtccggagt acaaataacg aaaccttttg cgccactggc agccaggctg tcgatcgcgt
     4861 tcaatgtttt ttcgccatcc ggcacggcaa tcttaataac ctcaaaccct aaatccttcc
     4921 cggctttatc ggcaaacttc cattcggtct ggaaccacgg ctcttccggt tgcttcacca
     4981 gaaaaccgag cttcaggttc tccgccatag cggattgtga cataacggct gccagaccaa
     5041 tggctgccag ggctttagta aatttgtgca tggttctctc cagctttagt gtcgttttgt
     5101 gtagggcaaa aacgaatgac attcgttaaa ttaatcggaa aacaaagcat taccttttaa
     5161 ctaaaagata agtgaccgtg ttgacatagt tttagcgaga aattaattct ccataggaga
     5221 gcaatatcac atcgcagaat tacagtgaga acgtgcataa atttagcggg aaaagacata
     5281 agggaaagcc aatttgtcag acaaattgtc gaatgcacag cagattaatc cataagatta
     5341 gcttggaaat ccttgttgtc tttggtaccc atgcgggatg tcttcttttt aaccagtcaa
     5401 taggccgcat tacctggcgt tgagtttttg aaatggtgta ataaccgcaa ctcaaagatg
     5461 tggaaaatgc acgtcattca tttcgccatt aattatcact gtgctcatta attaacagaa
     5521 cacgtataat gagagccatc tcgcaaaaat gaaaaaacgt tttataaaat catcatttca
     5581 tcaatgaaat gtaaaaatat ataaacttga tgatttaagc attttcttat acccgttcag
     5641 acgttattct tatttcagat catcgtcaga attgactcca cgatcacatt tcggaccggc
     5701 agaaaggaat tattctgcaa acagtaatta tggtgttttg atttatcttg cacctctcca
     5761 cttctggata taaggatatt aggtatggca accgctggaa tgcttctcaa actcaactct
     5821 caaatgaacc gcgagtttta cgcatccaat ctctaccttc acctgagtaa ctggtgttct
     5881 gaacagagtc tgaacggcac cgccactttc cttcgcgccc aggcacagag taatgtgacc
     5941 caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg ctacccccat cgttaaagcc
     6001 attgatgttc ccggtgaaaa actgaactct ctggaagaac tgttccaaaa aacgatggaa
     6061 gaatacgagc aacgttctag tacgttggca cagttagccg atgaagcgaa agaactgaat
     6121 gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag aacagcagca tgatggtctg
     6181 ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac ttgctggtat gtgcccagtg
     6241 cagaccgacc aacatgttct gaatgtcgtg tcgcatcagc tgcattgatc atacatcggc
     6301 gctaatgcat tgcgccgatg aagtttttga gaaaccgctg cctcatcttt ttgaagcagc
     6361 ggttttttaa tgggattcgc cttgtggggt aaacttgagt tcaatcagcg cgatggcttt
     6421 ttggattgcc cgcatggtga ccgggtctgc ggcggcggga tggttactaa agtcgatatt
     6481 cttcagctga ctggacattt tttcacgaac ttcaacgggc gcgattacat cgagaacatc
     6541 cagaatttgt ttgataacca actggcaagc aaccacatca gaaaccagtt cctgatcggc
     6601 attcagcggc tgggacatcg taaactcctg atagcatttt gaaaaccgtt atagtagcgg
     6661 cttcacatct tcagcgatag tcacatccac cgtcatcagg acacaaaaaa acctgccgga
     6721 gcaggttttt tgttatcgga acatattgcc tggcggtacg tctttgaacg tcttgcaata
     6781 gttattgaac atacttttca ggattttgcg cagtttcatc gcggcactcc gaccatttgt
     6841 tatacatgtg ttattttctt tgtgcttata atatgacaac catcacaaaa atcaatcttt
     6901 atgtgatgca aatcacacaa ataccccctt aatgttataa aaatgataat caaaaaacag
     6961 cccccccatt tctgacacct acagatggca agaaatagcg cctgccaggc gtcttttccg
     7021 gtcattgtcg cagcgctgta acgcgtaaaa tagtgctttc ttttactctt ctggctggac
     7081 catgagactt ctgattctga ctctttcatt aataacgctg gcggggtgta cggttactcg
     7141 ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt gtacggttgg tttatgacca
     7201 ggcttttttg cagcatgccc ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc
     7261 atgccagcag gaaggctata cccacgcaat cccctttggt cagccagtag gcaactgtag
     7321 cctttttgcg ggttccttat gtttgaatac ggaattcact ttgtcatatc agtgccatca
     7381 ttccgccttc cctgtctttc tctaaatatt ttaatcagcg aggggatctt cgctgattaa
     7441 aaaaatagcg gtaatgcgtt ttattcccat tcgtattttt aataattgaa gtttatattt
     7501 taccttttgc aaataataaa ataacaaatt atagtgacgc cacagcaaca aatataacct
     7561 ttgtggagca ctatcatgct gaaaccagaa atgattgaaa aacttaatga gcagatgaac
     7621 ctggaactgt actcttcact gctttatcag caaatgagcg cctggtgcag ctatcatacc
     7681 ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccaag aagagatgac gcatatgcag
     7741 catctgtttg attacctgac tgataccggc aatttaccgc gtattaatac cgttgaatct
     7801 ccgtttgctg aatattcctc acttgatgaa ttattccagg aaacctataa acacgaacaa
     7861 ttaatcaccc agaaaattaa cgaactggct catgctgcaa tgaccaatca ggactaccca
     7921 acatttaatt tcctgcaatg gtatgtttct gagcagcatg aagaagagaa actgttcaaa
     7981 tcgattattg ataaattaag cctggcaggc aaaagcggcg aaggtctgta ttttatcgac
     8041 aaagaactct ctaccctcga cacacaaaac taatactctc cggcggcagg tttactgccg
     8101 ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt cgaaaaaccg tctttcgaag
     8161 gtataaattt gccttttgct gccagaaacg ctaccagctc tcccgctgtc atcccttctg
     8221 ccgagcaggt gtgaaaacgt gcctgttcac caaaacgcgc tttaattgct gcttccagac
     8281 tggcatgcgt atattgctcg cctgattcaa tcatcatatt taacacttca tgaccgtgaa
     8341 tagagtccat cgttcctcct caaaaaaagc ctagcgtagc gattgccgct tatgaagact
     8401 ttgcgccaga gcagaactga atgcttttta ttgtacattt atatttacac catatgtaac
     8461 gtcggtttga cgaagcagcc gttatgcctt aacctgcgcc gcagatatca ctcataaaga
     8521 tcgtcaggac agaagaaagc gtgaaaaaca gaactctggg aagtgttttt atcgtggcgg
     8581 gaaccacaat tggcgcaggc atgctggcaa tgccgctggc tgcggcaggt gttggtttta
     8641 gcgtcacgtt aatcttgttg attgggcttt gggcgttgat gtgctacacg gcgctattac
     8701 tgctggaggt gtaccagcat gttccggcag ataccggtct gggcacgctg gcaaaacgct
     8761 atctgggacg ctacggtcaa tggctgacgg gcttcagtat gatgttctta atgtatgctc
     8821 tgactgcggc atacatcagc ggtgccggtg aattgttggc ctccagcatc agcgactgga
     8881 caggtatttc aatgtcggca accgctggcg tgctgttgtt cacttttgtt gccggtggcg
     8941 tggtttgtgt tggaacatcg ctggtcgatt tatttaaccg ttttctgttc agcgccaaaa
     9001 ttatttttct ggtggtgatg ctggtattgc tgctaccgca tattcacaaa gtgaatcttt
     9061 taaccctgcc gttgcaacag gggctggctc tgtcagcaat cccggtgatt tttacctcat
     9121 ttggttttca cggtagcgtg ccgagtattg tcagctatat ggatggcaac gttcgtaagc
     9181 tacgcagggt gtttataacc ggtagtgcga tccccctggt ggcatatatt ttctggcagg
     9241 tggcaacgct tggcagcatt gattcaacaa cctttatggg attgctggct aatcatgctg
     9301 gattaaacgg gctgttacag gcgttacgcg aaatggtggc ctctccgcat gttgagctgg
     9361 cagtgcattt atttgctgat ttagccctcg ccacgtcatt tctcggcgtt gcgttaggct
     9421 tatttgatta tctggctgat ttatttcagc gttcaaatac cgttggtgga cggttgcaaa
     9481 ctggtgcaat tacctttctg ccgccgttgg cgtttgcact gttttatcca cgaggatttg
     9541 tgatggcgct gggttacgcc ggtgtggcgc tggcggtact ggcattgatt atcccttcgc
     9601 tgttgacctg gcaaagcaga aagcacaatc ctcaggcggg ttaccgggtc aaaggtggtc
     9661 gtccggcgct ggtggtggtg tttctctgtg gtattgctgt gattggcgtg caatttttga
     9721 ttgcggcagg gttgttacca gaagtgaggt gatcagatag cctcaaattc cttattgggt
     9781 gccagaatta acgctgacac ccaatttggc ctcttaatgc aggcagcact gcttaaattt
     9841 cttaccacta ccgcacgggc aaggatcgtt acgccccggt ttctcttctg ctttgatcgg
     9901 ttgctgaaca gctttttcct gcggatgcgc catccagtac gcatgtagat caagcgccgc
     9961 cagtcgaatg gcatctacgc tctcttcaaa cgcttctggc gacatctttt ctacccgctc
    10021 gaagttttcc tcagtaccgt gcagcgcaat cgcctccagc gctggtttta acgaatcggg
    10081 caacgttgac cagtcagaaa gtgccacgcc ccgcatatag ccaaagcacc actcctcaac
    10141 aatcgtcagc tcgctgccat caacttctcg caagccgaat aacggctcaa actgctccgg
    10201 gaattcgttc agacgctctg cggtatcggc catatgttga aaagccaggt tcataaagcg
    10261 cgtcatctct ttctctgacg cccagcgcgg cacatagtca gccccacccc acacggcaac
    10321 cagccactgt tccggttcaa tctcttgcgg agaactcaac accgccgtca ataaatcgtc
    10381 cagctccgcc acatcaagga tggcgtggtt agtgttgtat ttggtcagaa tatcgtccag
    10441 ccattccaac tcactttcgt ttaacggtcc cgttttcata cgcttttcct tgtggatctc
    10501 aactcgccag cacctatctt acatgccggt ccgtatcaga gatacttttt gagtggcttt
    10561 gccggtgatt aaaaattaag gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc
    10621 gaaggtggtt tcacgacact gcttattgct ttgattttat tcttatcttt cccatggtac
    10681 ccggagcggg acttgaaccc gcacagcgcg aacgccgagg gattttaaat cccttgtgtc
    10741 taccgattcc accatccggg ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg
    10801 actagacgga tttgcaatcc gctacataac cgctttgtta acgcgccaaa ttcttcaggc
    10861 ctttcagcca gacatccgct tgacgccgat gtcttttaaa
//
LOCUS       DAJVOY010000135        10877 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVOY010000135 DAJVOY010000000
VERSION     DAJVOY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.135"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            286..1161
                     /locus_tag="OQL90_003068"
     CDS             286..1161
                     /locus_tag="OQL90_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCS1369753.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1169..1945
                     /locus_tag="OQL90_003069"
     CDS             1169..1945
                     /locus_tag="OQL90_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCS1369754.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2114..4375
                     /locus_tag="OQL90_003070"
     CDS             2114..4375
                     /locus_tag="OQL90_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1369755.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4444..5619
                     /gene="senB"
                     /locus_tag="OQL90_003071"
     CDS             4444..5619
                     /gene="senB"
                     /locus_tag="OQL90_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCS1369756.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            6078..6428
                     /locus_tag="OQL90_003072"
     CDS             6078..6428
                     /locus_tag="OQL90_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1369757.1"
                     /translation="MPSGRHTARIRTGHDSGTVSPFVHSTLSPLVPVYPVFITVIADH
                     DKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHR
                     QLHSCEAGSLKKGL"
     gene            6571..6792
                     /locus_tag="OQL90_003073"
                     /pseudo
     CDS             6571..6792
                     /locus_tag="OQL90_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6813..6875)
                     /locus_tag="OQL90_003074"
                     /pseudo
     CDS             complement(6813..6875)
                     /locus_tag="OQL90_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            7326..8738
                     /gene="ospC1"
                     /locus_tag="OQL90_003075"
     CDS             7326..8738
                     /gene="ospC1"
                     /locus_tag="OQL90_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCS1369758.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(8842..8990)
                     /locus_tag="OQL90_003076"
                     /pseudo
     CDS             complement(8842..8990)
                     /locus_tag="OQL90_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            9066..10763
                     /gene="ospD3"
                     /locus_tag="OQL90_003077"
     CDS             9066..10763
                     /gene="ospD3"
                     /locus_tag="OQL90_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCS1369759.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKI"
BASE COUNT         3385 a         2293 c         2294 g         2905 t
ORIGIN      
        1 catgtacatc aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt cagactccgg
       61 atagattcaa gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg gagtgaccat
      121 agatccgttt tccaagcgga ccccataata tcggctggta aacatcactc gctagtgcat
      181 tctttttgtt ggtaatgata attatttaca tttattcttt gatgttttat cataattaat
      241 gttttatcaa taaatatatg gaaatatatt gtttcagatg ggaatatgag aaagttcatc
      301 cttatttcta tgataacgtt agctagtgtt agtctgggag cctgcagaca gaatgtcacc
      361 ataaagcagg atgcgccagg gcagaaagct ttcctgattg acgggcagat ttacgatttg
      421 cacagtggca aaattatttc tagcagcgag cttttggcgg atcttgctac cgctcgacat
      481 cttattattg gtgaaaaaca cgataatgcc gagcatcatc agattgagct ttggcttatt
      541 caaaatctgc tcatccagcg accacagggg agtgtgttgc ttgagatgtt gacgtcagac
      601 caacagcccc gagttaacca agttaaatgt tggctgaaag ataatcctgt ggttcgagat
      661 agccgtgttc aggaactact taactggcaa aaagggtggt cctgggagat gtatggcgac
      721 atcgtcatgc agctattacg tgggccttac ccgctgctca atgccaacat tggccgggag
      781 cagatgttgg cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc cactgcacct
      841 gtggtacaag aagctctgcg tgaaaccatc atagctatgc atgaaggcaa ccttgaaagc
      901 cagcaactga cgtcaatgct ctctgttcag cagcagcggg atcgctacat ggcccgccag
      961 cttctgagtg ccccggtacc gtcgttgttg attgcaggtg gttatcacgc gtcaaaaaac
     1021 atgggcgtac cgttacacat ggaagatctt gccaccggca cccatccagt ggtgttgatg
     1081 cttgcagaaa aaggaatgaa catcacagta gatcatgctg actacgtgtg gtttgtagcg
     1141 ccagatacaa caaaaaggta aaccatggat gatgaatatt ctccacttcc cacagtcggt
     1201 aaaatggagc agctggttta tctgttctct actgcttcat ggactgattt tccttacgtt
     1261 tatctggcgt ttttccgaag ttcagcccgc tatgtctcct gcaccggcga tcatgctgca
     1321 atgggcagaa attatagagg ctccttcttc tccactttcg cttccggtgg gaatcgctca
     1381 acaggagtcc gcagtgacag aagaaaagca acaaactgag gacagacaac aacgtcctgt
     1441 cactgaagac agtgatgcaa ccattgaaat aactaggaag aaaaagtcat ccgatggaga
     1501 aaagaaaaag acacgtccac ctcgtaagat aaaggctcaa accagcgaca gcaaccccac
     1561 agccgtttcc agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta ttgctgcgcc
     1621 ttttaacagt gactcaacaa agcgagataa ttctgaggca tcttgggaaa gcagagtaaa
     1681 aggccacctt aaccgttata aacgttaccc tggcgatgcc agaaaacgtg cacgaaccgg
     1741 cacagccgtt gtgactttta ccgtaaacac ggaaggcact attgtcagta gtttcttgga
     1801 aatctcgtca ggaacccttt ctcttgaccg cgaagctatt gctgttttag aacgcgctca
     1861 accacttccc aaaccaccgc ctgaaatatt ggagggagga ttatttaaag taaaaatgcc
     1921 gattaccttt aagttgaagg agtaaaggga taattggccg ttcaagttca tttaactgaa
     1981 atttaatttt catttttatg ctgaacattt tatcaaactt ttccactgtc gaaaaaatca
     2041 aaaagacagt agatcataat taataaacaa atagttatac ttatctttat taatattata
     2101 tccaaggaaa tttatgaacg ttataaaact ggctatcggc tcaggcatat tattgctcag
     2161 ctgcggtgct tactcacaat ccatcagtga aaaaactaat tccgacaaaa aaggagcggc
     2221 agaattcagt ccgctcagcg tttctgtcgg gaagacgacc agtgagcagg aagctctcga
     2281 gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac ctgcaatcac ttgacgcaac
     2341 agtgcgtagt atgcctggta cttatactca aatagatcct ggtcagggag caatcagtgt
     2401 gaatattcga ggcatgagcg gatttggtcg tgtaaacact atggtcgatg gtattaccca
     2461 gagtttttac ggaacctcta cctccggaac aacggcgcat ggttcaacta acaatatggc
     2521 tggcgtactt atagatccta acttactggt agcagttgat gttacacgcg gtgacagcag
     2581 tggctctgaa gggatcaacg cccttgccgg tagtgcaaat atgcgtacta ttggcgttga
     2641 cgatgtaata tttaacggta atacatatgg ccttcgttca cgtttctctg tcggtagtaa
     2701 tgggctggga cgcagcggaa tgatcgccct tggtggaaaa agcgacgctt ttacggatac
     2761 gggaagcatt ggcgttatgg ctgctgtgag tggcagttct gtgtactcta atttctcaaa
     2821 tggttctgga attaacagca aagagtttgg ttatgataaa tatatgaagc agaaccccaa
     2881 atcccaactg tataaaatgg atatcagacc agacgaattt aacagcttcg aactttccgc
     2941 tcgaacctat gaaaataaat ttacacgtcg tgatataacc agtgacgact attacattaa
     3001 atatcattac accccttttt ctgaattaat tgactttaac gtaacggcca gtaccagtcg
     3061 cggtaatcaa aagtatcgtg atggctcgct gtatactttc tacaaaacct cagcgcaaaa
     3121 tcgttctgac gcgctggata tcaacaatac cagccggttc actgtcgcgg acaatgaact
     3181 ggagtttatg ctgggcagca aactgatgcg tacccgctat gaccggacca ttcactcagc
     3241 ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac aatccgttcg caccctccgg
     3301 ccagcaggat atttcagcgc tgtataccgg gctgaaggtt acgcgcggca tctgggaggc
     3361 agatttcaat ctcaactaca cacgtaacag gatcacaggg tacaagcccg cctgcgattc
     3421 acgcgttatc tgcgtgccac agggtagcta cgatattgac gataaagagg gtggcttcaa
     3481 cccttcagtt cagctttctg ctcaggtaac accatggctt cagccgttca ttggctacag
     3541 caaatccatg cgcgccccga acatccagga gatgttcttc tctaattcag gaggcgcatc
     3601 catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg caggcgggtt ttaacattga
     3661 taccagagat ttactggtcg aacaggatgc cctgcgcttt aaggctctgg cgtaccgcag
     3721 caggatccag aactacatct acagcgagtc ttatctggtt tgttctggag gtcgtaaatg
     3781 cagtatggct gaggtgattg gcaatgactg ggagggcatt agcgatgaat acagcgacaa
     3841 tatgtacatc tacgttaact cggcaagcga cgttattgca aagggcttcg aactggagat
     3901 ggattatgat gcaggttttg cttttggccg actctctttc agccagcagc aaacagacca
     3961 gccaacctcc atcgccagca cctactttgg cgcaggggat atgaccgaac tgcccagaaa
     4021 atacatgacg ctggatactg gtgttcgctt cttcgataac gcgttgaccc tgggcactat
     4081 cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat tttgagcagg acgaacatac
     4141 cggcgcaata atcaaacagg atttgccgca gatcccaacg attatcgatc tctatggtac
     4201 ttacgagtac aaccgcaacc tgacactgaa actttcggta caaaacctga tgaacagaga
     4261 ttattcggag gcgctgaata agctcaacat gatgccaggt cttggtgacg agagccaccc
     4321 agccaattcc gcgcgtggca gaacatggat atttggcggg gacattcgtt tctgatgaca
     4381 atttccgcca ggttcagaca atacgttttc tctcttatgt caattttatt gcaggaacga
     4441 aaaatgaata ttttcacttt atccaaagca ccgctatact tgttaatttc actattttta
     4501 cccacgatgg ccatggctat cgatccacct gaacgcgaac tttcgcgatt tgccctgaaa
     4561 acgaattacc ttcagtcccc tgatgaaggc gtctatgaac tggcgtttga taatgccagt
     4621 aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg aagccaataa aggctatctg
     4681 tattcgttta attcagattc gctgaaagtc gaaaataaat acacgatgcc ataccgggca
     4741 ttttcgctgg cgataaatca ggataaacat cagctctata tcggacacac ccagtcagcg
     4801 tccctgcgta tcagtatgtt tgacacccca accggtaaac tggtaagaac cagcgacagg
     4861 ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg agcatttgcg ccatatggtt
     4921 tacagccagg attccgatac cctgtttgtg agttatagca atatgctgaa aacggccgag
     4981 ggcatgaagc ctctgcataa gctgttaatg ctcgacggga cgacgcttgc cttaaaaggc
     5041 gaggttaagg atgcttacaa aggtacagcg tatggtctga cgatggatga aaaaacacag
     5101 aaaatctacg ttggcggaag agattacatc aacgaaattg atgcgaaaaa tcagacgctg
     5161 ctgcgtacca tcccgttgaa agatccgaga ccacaaatca caagtgtgca gaatctggcg
     5221 gtggactccg cttctgaccg tgcctttgtg gtggtattcg accatgacga tcgttccggt
     5281 acaaaagatg gcctctatat ttttgactta cgcgacggta aacagcttgg ctatgtgcac
     5341 acaggagccg gagctaacgc ggtgaaatac aatccgaaat ataacgaact gtatgtcacc
     5401 aacttcacta gcggcaccat cagcgtagtg gatgccacca aatacagcat cacccgtgaa
     5461 tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg acgatatgga taccctttac
     5521 attggcatca aagaaggctt taaccgcgat tgggatcctg atgtgtttgt ggaaggagct
     5581 aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat cgggggaaaa aacatgggat
     5641 aaaaactccg ttattccact aaactggccg gactaggaaa agcaaacatc tctgtcctgc
     5701 cggtacgcct gttacgccgc tccatgaggc tgaggaatct ccagaaaacg agacaggcac
     5761 agcattgtac gatcaactga tttgtgccaa taattcaatg aaatcacagc tatgcctgtt
     5821 cacgtatagg aaattgaaaa actaagcgat accactgtcc gcgtcttatg cctcgtgcgg
     5881 taagctgttt ttcacatcac gccggtgttt ttttctttct gtggacggtt ttaatactgt
     5941 aatcggccgt tttttatact taccccgatt cccgggcgca cttatcctgc atcaccccgg
     6001 gatatacctc agcagactga tatcccgcgc gtttttcttc agcccctcca cattcagtcc
     6061 cgcgatggcc cggcggtata ccatccggcc gccacacagc acgcattcga acgggtcacg
     6121 actcaggaac tgtttcacca tttgtgcata gcacactttc gccactggtt ccggtttatc
     6181 ctgtttttat aacggtaatt gcagaccatg acaaaccgca gccgtcaggc tgcctactcg
     6241 agtcacaagg ctctctgtgt ccgatatgcc ggcagcgcat tacccatgaa actggctgga
     6301 acgtacacca taaggtgaag aaagttatgg ggggcggtga agaattatct aaccttgtct
     6361 tgttacatcc aaattgccat cgacagttac acagttgtga agccggctct ctcaaaaagg
     6421 gcttataaag gcttgagcgg tgtaccggga aaccggtatg caccgttctt aggggagggc
     6481 agtccagcaa tgaactgtcc ttacccgaca aacatccaaa acgaacggaa cgtgctggaa
     6541 tccgcatata acccgttgta aagtgaggaa atggacacct cacttgctca tgagaacgcc
     6601 cgcctgcggg cactgttgca gacgcaacag gacactatcc accagatggc tgaatacaac
     6661 cgcctgctct cacagcggat ggcggcttat gcttccgaaa tcaaccgact gaaggcgctg
     6721 gttgcgaaac tgcagcgtat gcagttcggt aaaagcccag aaaaacttcg cgcaaaaacc
     6781 gaacggcaga ttccattttc acgggcgatc tacgccacac aggctctggg ttgcagtgac
     6841 tgctccatta aggcgatttt gaactcataa gggaagtaac aaaaaaactg caaaagtaca
     6901 ataattatat atggccaaac taacctctat taaaaaaaca tcagtttttc acacttagtt
     6961 gagcttcatg aaaaaatcat cgaggatttc attgcagaat acacattcca ctaactggaa
     7021 gcactccata agaggaatca gagaaagtat cgttttttta tagtaaaatt cattgctgtt
     7081 caataaaatt gatataatta ttaaattatg gaaataattt cacaaaaaag attaacttga
     7141 gttcttaagc atcggatttt agatattttg aaatatctgg catcacataa catcaaaagt
     7201 atataatacc cttatctaat tacatgcagt atattgtata tatactcaaa aattgtaatt
     7261 cataccctac tatatcttgt ggggaaagat attaattaaa actgttttca tataaggttc
     7321 attttatgaa tatatcagaa acactgaact cagcaaatac ccaatgcaat atagattcta
     7381 tggataacag attacataca ttgtttccaa aagtgacatc agtgcgaaac gctgcacaac
     7441 aaactatgcc agatgaaaaa aatttaaaag atagtgcaaa tattattaaa gatttcttta
     7501 ggaaaactat agcagcacaa agttatagta gaatgttctc tcaaggctct aactttaaat
     7561 ctttaaatat agcaattgat gcaccatcag acgctaaagc ctcatttaag gctattgagc
     7621 accttgacag attatcgaag cattatatat ctgaaataag ggaaaaactt catcctcttt
     7681 ctgcagagga actcaatttg ctttcgctaa ttattaattc tgatttaatc ttcagacatc
     7741 aaagtaattc tgatttgtct gataaaattt taaacattaa gtcattcaat aaaattcagt
     7801 ctgaaggaat atgcacaaaa cgaaacacat acgctgatga tataaaaaaa atagctaatc
     7861 atgactttgt gttttttggc gttgaaatct ctagccatca gaaaaaacac cccctgaata
     7921 caaaacatca cactgttgat tttggtgcaa atgcgtatat cattgatcat gactctccat
     7981 atggatatat gacattaacc gatcactttg ataatgctat tccacctgtt ttttaccatg
     8041 agcaccaatc atttttagat aaattttcag aggttaataa agaagttagt cgatacgtac
     8101 atggaagtaa aggaattata gatgtaccaa tattcaatac taaagatatg aagttagggc
     8161 tcggattata cctgattgac tttattagaa aaagtgaaga ccaaagcttc aaggagtttt
     8221 gctatggaaa aaatcttgcc cctgtggatc tggatagaat cataaacttt gtttttcagc
     8281 cagagtacca tatacctagg atggtaagta cagaaaactt caaaaaagtt aagattagag
     8341 aaatatcctt agaggaggct gttacagcat ctaattacga agaaattaac aagcaggtca
     8401 ctaacaaaaa aattgctctc cagactcttt ttctttcgat tactaatcaa aaagaggatg
     8461 tcgccttata tatattatct aattttgaga taactagaca agatgttatt tccataaagc
     8521 atgagttgta tgatattgag tatctactta gcgctcataa ttcaagctgt aaagtacttg
     8581 agtattttat caataaggga ttggttgatg taaacacaaa gttcaaaaaa actaatagtg
     8641 gggattgtat gttggataac gcaataaaat atgagaatgc agaaatgata aaactattat
     8701 tgaaatatgg tgcaacatct gacaataaat atatttaatc aaaattgaat atcgtttagc
     8761 aaaaggcagt atttgcacaa aatatcgata tattgtttat tgtcagtatg gctcaatgtg
     8821 ataatagttg gaaagtttga tgggtttcgc cccgttgtag cggtagtcga gctggaaggt
     8881 cttcaggcac tgcttacagc gatagagcag cccccctgaa ctggaatggc cgttccggat
     8941 accccctgag tttcagagta acggggacaa accacatcaa tctttgccat caatcatcca
     9001 aagggcaaag agtacaacaa cactaagtct gcatcacaac ccatcaatga aaggaatata
     9061 tacatatgcc atcagtaaat ttaatcccat caaggaaaat atgtttgcaa aatatgataa
     9121 ataaagacaa cgtctctgtt gagacaatcc agtctctatt gcactcaaaa caattgccat
     9181 atttttctga caagaggagt tttttattaa atctaaattg ccaagttacc gatcactctg
     9241 gaaaacttat tgtctgtcga cacttagctt cctactggat agcacagttt aacaaaagta
     9301 gtggtcacgt ggattatcat cactttgctt ttccggatga aattaaaaat tatgtttcag
     9361 tgagtgaaga agaaaaggct attaatgtgc ctgctattat ttattttgtt gaaaacggtt
     9421 catggggaga tattattttt tatattttca atgaaatgat ttttcattcc gaaaaaagca
     9481 gagcactaga aataagtaca tcaaatcaca atatggcatt aggcttgaag attaaagaaa
     9541 ctaaaaatgg gggggatttt gtcattcagc tttatgatcc caaccataca gcaactcatt
     9601 tacgagcaga gtttaacaaa tttaacttag ctaaaataaa aaaactgact gtagataatt
     9661 ttcttgatga aaaacatcag aaatgttatg gtcttatatc cgacggtatg tctatatttg
     9721 tggacagaca tactccaaca agcatgtcct ccataatcag atggcctaat aatttacttc
     9781 accccaaagt tatttatcac gcgatgcgta tgggattgac tgagctaatc caaaaagtaa
     9841 caagagtcgt acaactatct gacctttcag acaatacgtt agaattactt ttggcagcca
     9901 aaaatgacga tggtttgtca ggattgcttt tagctttaca aaatgggcat tcagatacaa
     9961 tcttagcata cggagaactc ctggaaactt ctggacttaa ccttgataaa acggtagaac
    10021 tactaactgc ggaaggaatg ggaggacgaa tatcgggttt atcccaagca cttcaaaatg
    10081 ggcatgcaga aactatcaaa acatacggaa ggcttctcaa gaagagagca ataaatatcg
    10141 aatacaataa gctgaaaaat ttgctgaccg cttattatta tgatgaagta cacagacaga
    10201 tacccggact aatgtttgct cttcaaaatg gacatgcaga tgctatacgc gcatacggtg
    10261 agctcattct tagcccccct ctcctcaact cagaggatat tgtaaatttg ctggcctcaa
    10321 ggagatatga caatgttccc ggacttctgt tagcattgaa taatggacag gctgatgcaa
    10381 tcttagctta tggtgatatc ttgaatgagg caaaacttaa cttggataaa aaagcagagc
    10441 tgttagaagc gaaagattct aatggtttat ctggattgtt tgtagccttg cataatggat
    10501 gtgtagaaac aattattgct tatgggaaaa tacttcacac tgcagacctt actccacatc
    10561 aggcatcaaa attactggca gcagaaggcc caaatggggt atctggatta attatagctt
    10621 ttcaaaatag gaattttgag gcaataaaaa cttatatggg aataataaaa aatgaaaata
    10681 ttacacctga agaaatagca gaacacttgg acaaaaaaaa tggaagtgat tttctagaaa
    10741 ttatgaagaa tataaaaatc tgaatattat ctcgtggtga tacactgaat ttgaggtaac
    10801 cggctcattt aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt
    10861 ttgaagtgga cactatc
//
LOCUS       DAJVOY010000136        10859 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVOY010000136 DAJVOY010000000
VERSION     DAJVOY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.136"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            83..424
                     /locus_tag="OQL90_003078"
     CDS             83..424
                     /locus_tag="OQL90_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1369760.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="OQL90_003079"
     CDS             complement(415..1341)
                     /locus_tag="OQL90_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1369761.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1431..2429
                     /locus_tag="OQL90_003080"
     CDS             1431..2429
                     /locus_tag="OQL90_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1369762.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="OQL90_003081"
     CDS             complement(2426..2644)
                     /locus_tag="OQL90_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1369763.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="OQL90_003082"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="OQL90_003082"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1369764.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIVGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="OQL90_003083"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="OQL90_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1369765.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="OQL90_003084"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="OQL90_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1369766.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="OQL90_003085"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="OQL90_003085"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1369767.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="OQL90_003086"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="OQL90_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1369768.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="OQL90_003087"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="OQL90_003087"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1369769.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..10828
                     /gene="crr"
                     /locus_tag="OQL90_003088"
     CDS             10319..10828
                     /gene="crr"
                     /locus_tag="OQL90_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1369770.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
BASE COUNT         2649 a         2804 c         2687 g         2719 t
ORIGIN      
        1 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctggat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
      421 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcac tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctggga attttccttt
     1861 caccattgct ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcaccca caatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccgatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc catgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt agtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagaag agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat agacaggctg
     5341 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctagg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactctc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctattaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttacagctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 aaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgt gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttccta ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcaac tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 gggtttgttc gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg cagaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    10741 ctccaacatg gacgaaatca aagaactgat caaactgccc ggtagcgtaa ccgtgggtga
    10801 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcg
//
LOCUS       DAJVOY010000137        10849 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVOY010000137 DAJVOY010000000
VERSION     DAJVOY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.137"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            155..502
                     /locus_tag="OQL90_003089"
     CDS             155..502
                     /locus_tag="OQL90_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS1369771.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            492..854
                     /locus_tag="OQL90_003090"
     CDS             492..854
                     /locus_tag="OQL90_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1369772.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            851..1348
                     /locus_tag="OQL90_003091"
     CDS             851..1348
                     /locus_tag="OQL90_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1369773.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(1356..2540)
                     /gene="emrD"
                     /locus_tag="OQL90_003092"
     CDS             complement(1356..2540)
                     /gene="emrD"
                     /locus_tag="OQL90_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1369774.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(2959..3048)
                     /gene="tisB"
                     /locus_tag="OQL90_003093"
     CDS             complement(2959..3048)
                     /gene="tisB"
                     /locus_tag="OQL90_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1369775.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            3613..3711
                     /gene="ivbL"
                     /locus_tag="OQL90_003094"
     CDS             3613..3711
                     /gene="ivbL"
                     /locus_tag="OQL90_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1369776.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            3817..5505
                     /gene="ilvB"
                     /locus_tag="OQL90_003095"
     CDS             3817..5505
                     /gene="ilvB"
                     /locus_tag="OQL90_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1369777.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQDIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            5509..5799
                     /gene="ilvN"
                     /locus_tag="OQL90_003096"
     CDS             5509..5799
                     /gene="ilvN"
                     /locus_tag="OQL90_003096"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1369778.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            5873..6463
                     /gene="uhpA"
                     /locus_tag="OQL90_003097"
     CDS             5873..6463
                     /gene="uhpA"
                     /locus_tag="OQL90_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1369779.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            6463..7965
                     /gene="uhpB"
                     /locus_tag="OQL90_003098"
     CDS             6463..7965
                     /gene="uhpB"
                     /locus_tag="OQL90_003098"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1369780.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            7975..9294
                     /gene="uhpC"
                     /locus_tag="OQL90_003099"
     CDS             7975..9294
                     /gene="uhpC"
                     /locus_tag="OQL90_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1369781.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            9432..10823
                     /gene="uhpT"
                     /locus_tag="OQL90_003100"
     CDS             9432..10823
                     /gene="uhpT"
                     /locus_tag="OQL90_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1369782.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
BASE COUNT         2381 a         2827 c         3057 g         2584 t
ORIGIN      
        1 cgtccttcag ggcgtggagg atgtcaattt gcgctatgat acccatctga cctccttgta
       61 gcggatggca aaagcatatc aggaaacggg cggacgaaat tcttaaaaaa aaccgctaaa
      121 gatagagcgt tagtgaaatt gcaaaggagc aacaatgaaa atttcccgcc ttggagaagc
      181 gcccgattac cgcttctcgc tggctaatga gcgtactttt ctggcgtgga tccgtaccgc
      241 actaggattt ctggcggcgg gggttgggct tgatcaactt gcgccagatt tcgccacgcc
      301 cgtcattcgt gaactgctgg cgttgttgtt gtgtctgttt tccggcgggt tagcaatgta
      361 tggctacctg cgctggttac gcaatgaaaa agcgatgcgt ctgaaagagg acctgcccta
      421 caccaacagc ttattaatca ttagcttaat attgatggtc gtggcggtga tcgttatggg
      481 actggtgttg tatgccggat agccgcaaag ccagacgcat tgccgaccca ggactgcagc
      541 cggagcgcac atcactggcg tggtttcgta ccatgctggg ctacggcgcg ttgatggcgt
      601 tggctatcaa acacaactgg caccaggcgg gcatgttatt ctggatttcg attggcattc
      661 tcgccatcgt ggcgctgatc ctctggcact acacccgtaa tcgcaattta atggatgtca
      721 cgaatagcga tttttcccaa tttcacgtag tccgtgacaa atttttgatc tccctcgcgg
      781 tgttatctct cgcaatactg tttgctgtaa cgcatataca tcaacttatc gtatttattg
      841 agagagtcgc atgacaggaa gtcaggtcat tgatgctgaa gaggacaggc ataaactggt
      901 tgtagagtac aaggatgctc tgcaacctgc cgatttttat cacaatttca aacagcgggg
      961 catccgctcc gtacagctca ttccctatct tgaatttgat gaccggggcg atctgacggc
     1021 cgcttcggtg acagcagagc tgtgggggaa atttttaatt gccctgttcg agtgttgggt
     1081 gcgggcagat atcagtcgta tctcgattga gctttttgac gccaccttac aaaaatggtg
     1141 tggcggcgaa aacccgcaac cgcgccgtga ctgccaggcg tgcgactggc atcgcctgtg
     1201 cccgcacgct cggcaggaga cgccagacag cgtgctttgt gcgggctatc aggcgtttta
     1261 ttcttactcc gcaccgcata tgcgggtgat gcgtgatctg ataaagcagc atcgttctcc
     1321 catggagctg atgacgatgc tgcggtgacg tgcgcttaaa cgggctgccc ctgatgcgac
     1381 atccgcgtcg ccagcggcag ccagcacagc acgatcaaca atcccattaa ggtcatcaat
     1441 aaccccaggc taccctgacc ggtttgcggc aacatcgcag agagcgacgc cagcacgccg
     1501 gaaccaatgt tttgcagacc gccgaccagc gcgccagccg tgcccgccag gaaggggaac
     1561 ggctccatcg cgccgctggt cgccagcgga aacagcatcc cggcaccgaa aaagaacagc
     1621 gcggcgggaa cgagcagcgt ccagacattc atcacgccaa accagtcggg gatccacatc
     1681 agcaagccag ccagcaggca gcagataacc gactgccaca ttaacgtgga gaagcgttta
     1741 ttgggacgtc cggcaaacca tgcgccaaaa aacgctgccg gaatcggcag aataaacaaa
     1801 atactgaccg tcatactgct cagccctaac accgcgccca tcagcacgcc ggagcaggct
     1861 tcaaaggcgg caatcccggc cagaccgcca atcagcatca gcaaataaca gttaaaaccg
     1921 ctgttaccga aaagcgtttt ataactggta agcaggcgcg tgcgcggtgc atcgaccgga
     1981 cgcgtttccg gcatccagcg ggccatactg aaggtcacac caacacaaag aaccaacaaa
     2041 aagagataac aggcgcgcca gttccacatc gtatccagca gaccgccgat tagcggtgcg
     2101 agcaacggac tgacgagaat ccccatgttt aacaggctgt tagcatggcg caactgtgtc
     2161 cgttcatata aatctcgcgg caaagtacgc gccattacgc cgccaacgcc ggtgcccatc
     2221 ccctgcatcg cgctggcggc aatcaatacc gtcaaactgg aggtcgtgac cgcgaccagc
     2281 gttgccagca taaaaatgga cattccgacg aggatcaccg gtcggcgacc cacacggtcg
     2341 gaaatcgggc cataaaacag ctgtgagaca ccgtaagtca gcagataagc gcccattacg
     2401 ctctgcaccg ccccttcacg gacgttgaga tcgcgcgcca tatcggcaat agctggaata
     2461 taaatggttt gcgccatctg accgacggcc acgagtaata ccaacatcaa taacaaattg
     2521 acgtttcttt gccttttcat tatcacggat gcttttataa aaataattag aataagtcac
     2581 tacgcattcc cataaatgcg ggagaggaat ctatcacatt gatgaatgtt agccagatat
     2641 acgccaggaa tggggaattg tttagcggca gaatatgtaa acaaaagcgg caataaatgt
     2701 tgtcgggagg gattacgtgc gaactgtaac cctctcccca aaggggcgag ggggaaacca
     2761 ccgttccctt tcagaaaatg agctaaacat tgcacgggac ggtcccctcg cccctttggg
     2821 gagagggcta gggtgagggg cacaggcagc gcaggtgcaa acattctgct ggtagaagaa
     2881 gcccccaact ccaacccgca cgctaaatat aaaaggggag cggttacccg ctcccctttg
     2941 gtgcgacttg aatctgaatt acttcaggta tttcagaaca gcatcaagca gttgcagtgc
     3001 tgcaacaatg agtttgagga taagaatggc gatatccacc aggctcatac gcgtctcctg
     3061 tggttcagga gcactgccag ctgacgtacc tttccgctgc ctgttgccag ctgttgtgca
     3121 cgcggtatct caaggagaaa cgttgtgctt gttgacataa cacagtgtgc tcacgcgggg
     3181 ttaaggcgct gacggcacca cccgtttcag ccaggactta gtgcgccggg taacgatgcg
     3241 gcccccgcat aatacattgc gtacagtgat attatagaag tttactgtat aaataaacag
     3301 tgatatttgg acaaaacgca aactgtgatc gatactcaac tatgccctaa atacgtcaaa
     3361 attcgtgccg aaattgcgcg ttctgcgcgg aacacgtata ctttcagtgt tgacataata
     3421 cagtgtgctt tgcggttacc agctgcaggc ggctgatgaa acctcgctcc ggcggggttt
     3481 tttgttacct gcaattcagt acaaaacgtg atcagcccct caattttccc ttgctgaaaa
     3541 attttccatt gtctcccctg taaagctgtg cttgtataaa tattgttaaa cacaaaacca
     3601 acaaggtccc caatgactac ttccatgttc aacgcaaaac tactaccaac tgcgctatcc
     3661 gccgcagtgg tcgtcgtgcg tgtggtggtg gtcgtcggca atgcgccgta gggactggaa
     3721 caacacacga ttccaaaacc ccgccggcgc aaaccgggcg gggtttttcg tttaagcacc
     3781 tcccggaaag tcggcccaga agaaaaggac tggagcatgg caagttcggg cacaacatcg
     3841 acgcgtaagc gctttaccgg cgcagaattt atcgttcatt tcctggaaca gcagggcatt
     3901 aagattgtga cgggcattcc gggcggttct atcctgcctg tttacgatgc cttaagccaa
     3961 agcacgcaaa tccgccatat tctggcccgt catgaacagg gcgcgggatt tatcgctcag
     4021 gggatggcgc gcaccgacgg taaaccggcg gtctgtatgg cctgtagcgg accgggtgcg
     4081 actaacctgg tgaccgccat tgccgatgcg cggctggact ccatcccgct gatttgcatc
     4141 accggtcagg ttcccgcctc gatgatcggc accgacgcct tccaggaagt ggacacctac
     4201 ggcatctcta tccccatcac caaacacaac tatctggtca gacatatcga agaactcccg
     4261 caggtcatga gcgatgcctt ccgcattgcg caatcaggcc gcccaggccc ggtgtggata
     4321 gacattccta aggatgtgca aacggcagtt tttgagattg aaacacagcc cgctatggca
     4381 gaaaaagccg ccgcccccgc ctttagtgaa gaaagcattc gtgacgcagc ggcgatgatt
     4441 aacgctgcca aacgcccggt gctttatctg ggcggcggtg tgatcaatgc gcccgcacgg
     4501 gtgcgtgaac tggcggagaa agcgcaactg cctaccacca tgactttaat ggcgctgggc
     4561 atgttgccaa aagcgcatcc gttgtcgctg ggtatgctgg ggatgcacgg cgtgcgcagc
     4621 accaactata ttttgcagga ggcggatttg ttgatagtgc tcggtgcgcg ttttgatgac
     4681 cgggcaattg gcaaaaccga gcagttctgt ccgaatgcca aaattattca tgtcgatatc
     4741 gatcgcgcag agctgggtaa aatcaagcag ccgcatgtgg cgattcaggc ggatgttgat
     4801 gacgtgctgg cgcaattgat cccgcaggtg gaagcgcaac cgcgtgcaga gtggcaccag
     4861 ttggtagcgg atttgcagcg cgaattcccg tgtccaatcc cgaaagcgtg cgatccatta
     4921 agccattacg gcctgatcaa cgccgttgcc gcctgtgtcg atgacaatgc gattatcacc
     4981 accgatgtgg gtcagcatca gatgtggacc gcgcaagctt atccgctcaa tcgcccacgc
     5041 cagtggctga cctccggtgg gctgggcacg atgggttttg gcctgcctgc ggcgattggc
     5101 gcggcgctgg cgaacccgga tcgcaaagtg ttgtgtttct ccggcgacgg cagcctgatg
     5161 atgaatattc aggagatggc gaccgccagt gaaaatcagc tggatgtcaa aatcattctg
     5221 atgaacaacg aagcgctggg gctggtgcat cagcaacaga gtctgttcta cgagcaaggc
     5281 gtttttgccg ccacctatcc gggcaaaatc aactttatgc agattgccgc cggattcggc
     5341 ctcgaaacct gtgatttgaa taacgaagcc gatccgcagg ctgcattgca ggatatcatc
     5401 aatcgccctg gcccggcgct gatccatgtg cgcattgatg ccgaagaaaa agtttacccg
     5461 atggtgccgc caggtgcggc gaatactgaa atggtggggg aataagccat gcaaaacaca
     5521 actcataaca acgtaattct ggagctcacc gttcgcaacc atccgggcgt aatgacccac
     5581 gtttgtggcc tttttgcccg ccgcgctttt aacgttgaag gcattctttg tctgccgatt
     5641 caggacagcg acaaaagcca tatctggcta ctggtcaatg acgaccagcg tctggagcag
     5701 atgataagcc aaatcgataa gctggaagat gttgtgaaag tgcagcgtaa tcagtccgat
     5761 ccgacgatgt ttaacaagat cgcggtgttt tttcagtaac cgctcaaggc ttgaacaaca
     5821 tcgcgcttat cgttaaggta agcgcgtttt ttttacccgc caggacaaga ccatgatcac
     5881 cgttgccctt atagacgatc acctcatcgt ccgctccggc tttgcgcagc tgctggggct
     5941 ggaacctgat ttgcaggtag ttgccgagtt tggttcgggg cgcgaggcgc tggcggggct
     6001 gccggggcgc ggtgtgcagg tgtgtatttg cgatatctcc atgcccgata tctccggtct
     6061 ggagctgcta agccagctgc cgaaaggtat ggcgacgatt atgctctccg ttcacgacag
     6121 tcctgcgctg gttgagcagg cgcttaacgc gggggcacgc ggctttcttt ccaaacgctg
     6181 tagcccggat gaactcattg ctgcggtgca tacggttgcc acgggcggct gttatctgac
     6241 gccggatatt gccattaaac tggcatccgg tcgtcaggac ccgctaacca aacgtgaacg
     6301 ccaggtggcg gaaaaactgg cgcaaggaat ggcggtgaaa gagattgccg ccgaactggg
     6361 cttgtcaccg aaaacggtac acgtccatcg cgccaatctg atggaaaaac tgggcgtcag
     6421 taacgacgta gagctggcgc gccgcatgtt tgatggctgg tgatgaagac gttgttctcc
     6481 cgcttaatta ccgttattgc ctgctttttt atcttctctg ccgcatggtt ttgcctgtgg
     6541 agtatcagcc tgcatctggt tgagcgccct gatatggcgg tgctgttatt tccgtttggt
     6601 ctgcgtctgg ggctaatgct gcaatgcccg cgcggatact ggccggtgct gctgggcgcg
     6661 gagtggctgc tgatttactg gctaacgcag gcggtcggtt taacccattt tccgttattg
     6721 atgatcggta gtttactgac gttactgccc gtagcgctga tctcgcgcta tcgccatcag
     6781 cgtgactggc gcaccttgct gttacagggg gcggcgttaa cggcggcggc gttgttgcaa
     6841 tcgctgccct ggctttggca cggcaaagag tcgtggaatg cgctgttgct gactttaact
     6901 ggcggcctga cgctggcccc gatatgtctg gtgttctggc actatctcgc caataacacc
     6961 tggctgccgc tcggcccgtc actggtttct cagccaatca actggcgcgg gcgacatctg
     7021 gtctggtact tgctgctgtt tgttatcagt ctctggctcc agttgggatt gccggacgaa
     7081 ctgtcgcgct ttacgccatt ctgtctggcg ctgccgatta tcgcgctggc ctggcactat
     7141 ggttggcaag gggcgctgat tgcgacgttg atgaacgcca tcgcgctgat cgccagtcaa
     7201 acctggcgcg atcatccggt ggatttattg ctctcgctgc tggtgcaaag tctgacaggg
     7261 ttgttgcttg gcgctggcat ccagcggttg cgtgaactta accagtcgct gcaaaaggaa
     7321 ctggcgcgca atcagcatct ggctgaacgg ttgctggaaa ccgaagagag cgtgcgccgt
     7381 gatgtggcgc gtgagctgca tgatgatatc ggtcagacca tcactgctat tcgtactcag
     7441 gcgggcattg ttcagcggct ggcggcagat aacgccagcg tgaagcagag cgggcagctc
     7501 atcgaacaac tatcgctggg cgtttacgac gcggtgcgcc gtttgttggg tcggttacgt
     7561 ccgcgccagt tggatgatct caccctggag caggccatcc gctcactgat gcgggaaatg
     7621 gagctggaag ggcgcggtat tgtcagccat ctcgaatggc gaatcgatga atcagcgtta
     7681 agcgaaaacc agcgcgtgac gctgtttcgt gtctgccagg aagggctgaa caacattgtg
     7741 aaacatgctg atgccagcgc ggtcaccctg caaggctggc agcaggatga acggttgatg
     7801 ctggttattg aagacgatgg cagcggtttg ccgccgggtt ccgggcaaca aggttttggc
     7861 ctcaccggaa tgcgcgagcg cgtaacggcg ctgggtggca cattacacat ttcctgtctg
     7921 cacggcacgc gtgtcagcgt ttctctacct caacgctatg tctaaggttt gatgatgttg
     7981 ccgtttctga aagcgcctgc cgatgcgcca ttaatgactg ataaatatga aattgatgcc
     8041 cgctatcgct actggcgtcg gcatattctg ctgaccatct ggctgggtta cgcgctgttt
     8101 tacttcacgc ggaaaagttt taacgccgcc gtaccagaaa tccttgctaa cggcgtgctc
     8161 agccgtagcg atatcggcct gttagcgacc ctgttttaca ttacctatgg cgtgtcgaag
     8221 tttgtctccg gcattgtcag cgatcgctca aatgcccgtt attttatggg gatagggctt
     8281 atcgccacgg gcattatcaa cattctgttt ggcttctcga cgtcgctatg ggcgtttgcc
     8341 gtactctggg tgctgaacgc ctttttccag ggctggggtt caccggtgtg tgcgcgtctg
     8401 ttaacggcct ggtattcacg taccgagcgc ggtggttggt gggcattatg gaacacggcg
     8461 cataacgtcg gcggcgcact cattcccatt gtgatggcag cggctgcact acattacggc
     8521 tggcgtgccg ggatgatgat tgctggttgt atggcgatcg tcgtgggaat ttttctctgc
     8581 tggcggctgc gcgatcgccc gcaggcgtta ggtttaccgg cggtcggtga atggcgacac
     8641 gacgcgctgg agatagctca acaacaagaa ggggcagggc tgacgcgtaa agagatcctc
     8701 accaaatatg tgttgctgaa tccgtatatc tggctgcttt cgttttgcta tgtgctggtc
     8761 tatgtggtcc gggcggcgat caacgactgg ggcaatttgt atatgtccga gacgctgggc
     8821 gtcgatctgg tcacggcgaa tacggcagtg acgatgtttg aactgggtgg atttatcggt
     8881 gcgctggtag cgggttgggg ctcggacaaa ttgtttaacg gcaaccgagg gccgatgaat
     8941 ttgattttcg ccgccggaat tttgctttca gtcggctccc tgtggctgat gccatttgcc
     9001 agctacgtga tgcaggcaac ctgcttcttc accattggtt tttttgtctt tggcccacag
     9061 atgttaatcg gtatggcggc ggcagagtgt tcccacaaag aggcggcagg ggcggcgacg
     9121 gggtttgtcg gcttgtttgc ttatctgggg gcgtcgcttg ctggttggcc gctggcgaaa
     9181 gtactcgata cctggcactg gagcggattt tttgtggtta tctctatcgc cgccgggatt
     9241 tccgcactgc tgttactgcc ctttttgaac gcccagacac cgcgcgaagc gtgatgcatc
     9301 tcaccttttc acttcatatc cggcaaaact aagaaatttt ccaggttttg cctggacgct
     9361 atctcaggcc tgatttgctg ctgattttta caatgcatgc ctcacgcagg tattcatttc
     9421 aggagtaacc catgctggct ttcctaaacc aggttcgcaa gccgaccctg gaccttccgc
     9481 tcgaagtgcg gcgcaaaatg tggttcaaac cgttcatgca atcctacctg gtggtcttta
     9541 tcggctacct gacgatgtac ctgattcgca agaactttaa catcgcgcag aacgatatga
     9601 tttcgaccta cgggttgagc atgacgcagc tggggatgat cggcctgggt ttctccatca
     9661 cttatggcgt gggtaaaacg ttggtttcat actacgccga cggcaaaaac accaaacaat
     9721 tcctgccgtt catgctgatc ctgtctgcca tttgtatgct gggcttcagt gccagtatgg
     9781 gcagcggctc ggttagcctg ttcctgatga ttgccttcta cgccttaagc ggctttttcc
     9841 agagtaccgg cggttcgtgc agttactcca ccatcaccaa atggacgccg cgtcgtaaac
     9901 gcgggacatt cctcggtttc tggaatattt ctcacaacct tggcggtgca ggcgcagcag
     9961 gtgtggcgct gttcggggca aattacctgt tcgatggcca tgtcatcggc atgtttatct
    10021 tcccgtcgat tatcgcgctg attgtcggtt ttatcggcct gcgttacggc agcgactccc
    10081 cagaatctta tggcctcggc aaagctgaag aactgttcgg cgaggagatc agcgaagagg
    10141 acaaagagac agaatctacc gatatgacca agtggcagat ctttgttgag tatgtgctga
    10201 aaaacaaagt gatctggctg ctgtgcttcg ccaacatttt cctctatgtg gtacgtattg
    10261 gtatcgacca gtggtcaacc gtatacgcgt tccaggaact gaaactctct aaagcggtgg
    10321 cgattcaggg ctttacgctg tttgaagctg gtgcgctggt cggtacgctg ctgtggggct
    10381 ggctctctga cctggcgaac ggtcgccgtg gcctggtggc ctgcatcgcg ctggcgctga
    10441 ttatcgccac gctcggtgtg tatcaacatg ccagcaacga atatatctat ctggcttctc
    10501 tctttgcgtt gggtttcctg gtctttggcc cgcaattgtt gattggtgtg gctgctgttg
    10561 gctttgtacc taaaaaagcg attggcgctg ccgatggtat taaaggcacc tttgcttacc
    10621 tgattggtga cagctttgcc aagttaggtc tgggaatgat tgccgatggg acgccggtat
    10681 tcggccttac cggctgggca ggcaccttcg ccgcacttga tatcgccgcg attggttgta
    10741 tctgcctgat ggcgatagtg gcggtgatgg aagaacgcaa aatccgccgc gagaaaaaaa
    10801 ttcagcagtt gacagtggca taaacgtaac tggtgacttt tgcccggca
//
LOCUS       DAJVOY010000138        10689 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVOY010000138 DAJVOY010000000
VERSION     DAJVOY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.138"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(81..230)
                     /gene="hokB"
                     /locus_tag="OQL90_003101"
     CDS             complement(81..230)
                     /gene="hokB"
                     /locus_tag="OQL90_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1369783.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            629..2242
                     /gene="trg"
                     /locus_tag="OQL90_003102"
                     /pseudo
     CDS             629..2242
                     /gene="trg"
                     /locus_tag="OQL90_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(2280..3202)
                     /locus_tag="OQL90_003103"
                     /pseudo
     CDS             complement(2280..3202)
                     /locus_tag="OQL90_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3419..4762
                     /locus_tag="OQL90_003104"
     CDS             3419..4762
                     /locus_tag="OQL90_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1369784.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGPLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4987..6642
                     /gene="opgD"
                     /locus_tag="OQL90_003105"
                     /gene_synonym="mdoD"
     CDS             4987..6642
                     /gene="opgD"
                     /locus_tag="OQL90_003105"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1369785.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6782..7006
                     /locus_tag="OQL90_003106"
     CDS             6782..7006
                     /locus_tag="OQL90_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1369786.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            7069..7605
                     /gene="rimL"
                     /locus_tag="OQL90_003107"
     CDS             7069..7605
                     /gene="rimL"
                     /locus_tag="OQL90_003107"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1369787.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7600..8580)
                     /locus_tag="OQL90_003108"
                     /pseudo
     CDS             complement(7600..8580)
                     /locus_tag="OQL90_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8704..9696
                     /gene="tehA"
                     /locus_tag="OQL90_003109"
     CDS             8704..9696
                     /gene="tehA"
                     /locus_tag="OQL90_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1369788.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9693..10286
                     /gene="tehB"
                     /locus_tag="OQL90_003110"
     CDS             9693..10286
                     /gene="tehB"
                     /locus_tag="OQL90_003110"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1369789.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10589..>10689
                     /locus_tag="OQL90_003111"
     CDS             10589..>10689
                     /locus_tag="OQL90_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCS1369790.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSG"
BASE COUNT         2734 a         2495 c         2709 g         2751 t
ORIGIN      
        1 actaactgaa gtgtaaaagg ggtgccatga gaaaatgaat ctgctagtca gatgcgcggg
       61 gaaaatcccc gcgcttgccc ttacctggac gtgcaggcca tgagcgcagc aacctcctta
      121 tcaccgtccc ggaaccgcag ttcgtagagc gtttgtcggg tcaggagtgt gaatgtcaga
      181 atcgtaatgc agataatgag cagacacacc accagagggt tgtgcttcat agcctttctc
      241 cttgccggat ggcgggtaag aggctaagat ctgaattgct aggttcattc gttggcctcg
      301 gttgatagaa atatcggtcg gggccttcgt ctttctgatt cccggttagc ctgaaaacag
      361 aaagtctcag gcacccgcag gcatcctatg aggtttcctt agggacgaaa ataatcactt
      421 cacgaaattg cgtgctgttt tccagaattt ttcgtcattc ggattagcca gtttagccat
      481 tcgttactct cttcattcca atagcattaa ttttctctgc aataattgtt gtaaaaatgt
      541 gacgcaaaga ggtttttggt cataagtaat taccgtcaag tgccgatgac tttctatcag
      601 gagtaaacct ggacgagaga caacggtaat gaatacaact ccctcacagc gattaggttt
      661 tttgcatcac atcaggctgg ttccgttatt tgcctgcatt cttggcggta tcttagttct
      721 attcgcatta agttctgctc tggcaggccg atcgcgatca gcgtgatgtt actgcggaga
      781 ttgagattcg gaccgggtta gcgaacagtt cagatttttt gcgttcagcc cggatcaata
      841 tgattcaggc cggggctgcg agtcgtattg cggaaatgga agcaatgaag cgaaatattg
      901 cgcaagccga atcggagatt aaacagtcgc agcaaggtta tcgtgcttat cagaatcgac
      961 cggtgaaaac acctgctgat gaagccctcg acactgaatt aaatcaacgc tttcaggctt
     1021 atatcacggg tatgcaacct atgttgaaat atgccaaaaa tggcatgttt gaagcgatta
     1081 tcaatcatga aagtgagcag atccgaccgc tggataatgc ttataccgat attttgaaca
     1141 aagccgttaa gatacgtagc accagagcca accaactggc ggaactggcc catcagcgca
     1201 cccgcctggg tgggatgttc atgattggcg cgtttgtgcg tgccctggtc atgacgctga
     1261 taacatttat ggtgctacgt cggatcgtca ttcgtccact gcaacatgcc gcacaacgga
     1321 ttgaaaaaat cgccagtggc gatctgacga tgaatgatga atcggcgggt cgtaatgaaa
     1381 tcggtcgctt aagtcgtcat ttacagcaaa tgcagcattc actggggatg acagtaggga
     1441 ctgttcgaca gggcgcggaa gagatttatc gtggcaccag cgaaatttca gctggcaatg
     1501 cggacctgtc atctcgcacc gaagaacaag cggcggctat cgaacaaact gccgccagca
     1561 tggagcaact cactgcgacg gtgaaacaga atgcggataa cgcgcatcat gccagcaaac
     1621 tggcgcaaga ggcttctatt aaagccagcg atggcgggca gacggagtaa aaacgatggg
     1681 cgctatctcc acgagttcga agaaaatttc tgagatcacc gccgtcatca acagtattgc
     1741 tttccagacg aatattctgg cactgaatgc tgccgttgaa gccgcgcgag cgggtgagca
     1801 agggcgtgga tttgccgttg tcgccagcga agtacggaca ctcgcaagtc gcagcgctca
     1861 ggcggcgaaa gagattgaag gcttgatcag tgaatcagtc aggttaattg acctggggtc
     1921 ggatgaggtg gcaacggccg ggaaaaccat gagcactatt gttgatgccg tcgcgagtgt
     1981 cacacatatc atgcaggaaa tcgccgccgc ctcggatgaa caaagtagag gcataacgca
     2041 ggttagccag gcgatttctg aaatggataa ggtgacgcaa cagaatgctt ctctggtaga
     2101 agaggcctca gcggcggcgg tgtcccttga agaacaggcg gcacgattaa ctgaggcggt
     2161 ggatgtattc cgtctgaaca aacattctgt gtcggcagaa cctcgcggag cgggtgaacc
     2221 agttagtttc gctacggtgt gaaaatgttc aaggagggat cgacagatcc cttcaccttt
     2281 cagaacggca ttgattttcg aatagcgtta atcatcaact ggcaaccaga agagaacatc
     2341 gcatctacgc gggtcagtat tccaatcggt tcgcctgcac catgtcccgg aacaggctgg
     2401 gccaccagcg tggcatgacg caggtcgtct tttaccgcgc cagaagggac aaaccacacg
     2461 tagtcgtatt caaccgtaag ttgacgagat agcgaagcag acagcgtttc gatacaaccc
     2521 gaaggaattt tacatccctg gctctgtact aatgcatctg aatgctggcg tggcgcagtg
     2581 ccttctggtg atacaacgac cggccatttc agcactcggc ttagcgttac gttctcctga
     2641 agtagcgggt gattagggcg gacaaccagc ttcagcgatt caagaaacag cagttcgtaa
     2701 ttaagcccgg tcatcagttc aggatctgac atccgaccaa tgccgatatc gatttccccg
     2761 gttttcaaac ccgccagaat catagggtta ctcattgtcg caacttgcaa ggtcgtctct
     2821 ttttgttgct gatgaaactg acctataacc gaaggtaata tccccagtgc cgcagtaggt
     2881 agtgcaccaa tcctgacgac atcattatta agaccttctt tacgatgaag cgactgtccg
     2941 gcagtgttga tggcgtcaag aactctgact gcatgcgtta aaaattgttc gccgggtaag
     3001 gtaagttgcg ccccctgacg accacgctca aacaagcgag cgcccgtcag ctgctacagt
     3061 tcattcaatg tcttagagag cgcaggttga ctcaaattaa gggtttcagc cgcgccccca
     3121 aagttccttg ttgtgcgaca gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac
     3181 taaacagact atttttttcc ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg
     3241 aagttgtttg attatgataa cttgattgca aaatataatt aacaattaaa gcaattgtgt
     3301 tacagcaaaa tggataatat tgatgttttc gcggcgagat cacagtttgt aaattcttcc
     3361 cgccagagtg aatgcggtta cctacactcc agattactga ccactggagg cagacactat
     3421 ggcgaacagc atcacggcgg atgagattcg ggaacagttt tcgcaggcaa tgtcagccat
     3481 gtaccagcaa gaagttccgc agtacggccc gctgctggaa ctggtagctg atgtgaatct
     3541 ggctgtgctg gaaaacaatc ctcaactgca cgaaaaaatg gtaaatgcag atgagctggc
     3601 gcgactgaat gttgaacgtc atggggcgat tcgcgttggg actgcacaag agcttgctac
     3661 tcttcggcgg atgtttgcca ttatggggat gtacccggtg agctattacg atctctcgca
     3721 ggcagggggg ccggtacatt cgacagcatt tcggcccatt gatgatgctt ctctggcgcg
     3781 taatcccttc cgcgttttta cctccttact ccgccttgag cttatcgaga acgaaatttt
     3841 gcgccagaaa gcggcggaga ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca
     3901 actgttagag gaatatgagc agcagggcgg ttttaacgaa acacaggcac aggaatttgt
     3961 gcaagaggcc ctggaaacgt ttcgctggca ccagtcagca acggtagatg aagaaaccta
     4021 tcgcgccttg cacaacgaat atcggttgat tgctgatgtg gtctgttttc ctggatgcca
     4081 tatcaaccac ctgacgccac gtacgctgga tattgaccgg gtgcagtcga tgatgcctga
     4141 atgcggaatt gaacccaaaa ttctcatcga ggggccgccg cgccgcgagg taccgatttt
     4201 actacgccag accagcttta aagcactgga agagacggtg ttgtttgcgg ggcagaaaca
     4261 gggcacgcat accgcgcgct ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa
     4321 agggcgacaa ctgtatgatg atcttctgcg taacgctgga accgggcagg ataatctcac
     4381 tcaccaaatg catttacagg aaaccttccg cacttttcct gacagtgagt ttttaatgcg
     4441 tcagcaaggg ttggcatggt tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca
     4501 ggcgattcat cttggagacg atccacagcc tttaattgaa cgtggttggg tagtggcgca
     4561 acccatcacc tatgaagatt tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct
     4621 gggtaatgaa acgcagacac gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc
     4681 gttgggttgt ccggttttgg atgagttcca gctttaccag gaagcggaag aacgcagtaa
     4741 acgtcgctgt ggtttgcttt aaattctgac catccgcctt tgcaaaaatt tgcctgattt
     4801 tacaaacgaa tcaggctcat cccatcgaca taaaaaaaat gccgatttat gcatattctc
     4861 tcagttcaac aattggatta ttaataaata ttgtctagag tgagcggtca taaataagca
     4921 ctttcttgcc gctgaaaacg accagcgcgg gaccattcac aacaccagaa ggactcactt
     4981 tcaggtatgg atcgtagacg atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt
     5041 accagcggca ttgcttctct tttttctcag gcggcattcg cggcagattc tgatattgcc
     5101 gacgggcaaa cccagcgttt tgacttctcc attctacagt caatggcgca cgacttagcg
     5161 caaacagcgt ggcgtggtgc gcctcgtccg ttacctgaca cgctggcgac aatgacgccg
     5221 caggcttata acagtattca atacgacgcc gaaaaatcgc tctggcataa cgttgagaac
     5281 cgtcaactgg acgctcagtt cttccatatg ggaatgggat tccgtcgccg cgttcgtatg
     5341 ttttctgtag atccagcaac acatctggcg cgtgaaattc actttcgccc ggagttgttc
     5401 aaatacaacg atgcgggtgt tgatacaaaa caattagaag ggcaaagcga tctcggcttt
     5461 gccggttttc gcgtgtttaa agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc
     5521 ggcgcgagtt atttccgcgc cgttgatgat acatatcaat acggtttgtc ggcccgcggc
     5581 ctggcgatcg acacttacac cgacagtaaa gaagagttcc ccgactttac cgccttctgg
     5641 tttgatacgg taaaaccggg ggcaactacc tttaccgttt atgcgttgct cgatagcgcc
     5701 agcattactg gtgcctataa gttcactatc cattgtgaga aaagtcaggt gattatggat
     5761 gtggaaaatc acctgtatgc gcgcaaagac attaaacagc tgggcattgc gccgatgacc
     5821 agtatgttca gctgcggtac taatgaacgt cggatgtgcg atacaattca tccgcaaatt
     5881 catgactctg atcgtctgtc catgtggcgg ggcaacggcg agtggatttg ccgtccgctg
     5941 aataatccgc aaaaattgca gttcaatgct tacaccgaca acaacccgaa agggtttggt
     6001 ttattgcaac tggatcgtga cttctcccat tatcaggaca ttatgggctg gtataacaaa
     6061 cgcccaagtc tgtgggtgga accgcgtaac aagtggggta agggcaccat cggcctgatg
     6121 gaaatcccaa caacgggcga aacgctggat aacattgtct gtttctggca gccagaaaaa
     6181 gctgtaaaag caggtgatga gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg
     6241 cctgttcatt gcccattagc gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg
     6301 gaaggttggg cgccaggtga acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat
     6361 ttcgttggtg gtgatctgaa agctgccgcg ccaaaaggca ttgagccggt gattacgctt
     6421 tccagtgggg aagcgaagca aatcgaaatt ctctatattg aacccatcga tggttatcgt
     6481 attcagtttg actggtatcc gacttcggac tccactgatc cggtcgatat gcggatgtat
     6541 ctacgttgtc agggggacgc tatcagtgaa acatggctgt atcagtattt cccgccagcg
     6601 ccggataaac gtcagtatgt tgacgaccgc gtgatgagtt aattgttttt tcttcggcac
     6661 cttcttcggg aggtgccgtc tggttaaaca cgatcccgct cgcatttttc cctaagttaa
     6721 atgagtaatc tgatggtgtg tatttcagat acaccttgtc agccactaac agggagtgcg
     6781 tatgtttcca gaataccgag atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat
     6841 gtccttgttc gataaacaca ataaacttga tcatgaaatt gccagaaagg aaggtttcga
     6901 cggtcgaggg tacaatgcgg aagtggtccg catgaaaaaa caaaagctac agttaaaaga
     6961 tgagatgctc aaaatcctgc agcaggagag cgtcaaaggg gtgtaaactt tcctaagccg
     7021 ccagccaggc ggctttttta acaacagcat ggattgactg gagataagat gactgaaacg
     7081 ataaaagtaa gcgaatcact tgaattacat gctgttgcgg aaagtcacgt cacacctctt
     7141 tatcagttaa tctgtaaaaa taaaacctgg ttacagcagt cgctaaactg gccgcagttc
     7201 gttcagagtg aagaggacac gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc
     7261 ggctatgcca aaatgttcat gattttcaaa gaagatgaac ttatcggcgt tatctcgttt
     7321 aatcgtattg aaccactgaa taaaactgct gaaatcggct actggctgga cgaatctcat
     7381 caggggcagg ggatcatttc tcaggcgctg caggcattga ttcatcatta cgctcagtct
     7441 ggtgaactta gacgcttcgt gatcaaatgt cgggtggaca atccgcaaag caaccaggtc
     7501 gctttgcgca atggttttat ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat
     7561 gcctatgatg atgtgaactt atacgcgcgt attatcgatt cataataacc ctgccagcgg
     7621 cgttcgcgta atgcgctcct cgccgttaat gacttttgga ccacggacat gaaccgtgtc
     7681 gccatcaaag gcttcaacga cggcgtggtc ggttaactcc acatggtttt caattatcac
     7741 cgcacctgaa atacggctct caccttgtat tacaacgtgc tcgtcgagca gaatcggttc
     7801 accacgtacc acggcatttc cgccaatcaa gacatgatgt tttaacacac aattaccttc
     7861 cacaatggca tattccgcca cctgcgaact gtaatgaatc gtcggaatgg catcttcttc
     7921 tatgccagct ttcacctgcg cgtgaccgta gactttagcg caatcgcata gccagacgtt
     7981 gttctcttca ttaccttcaa cactggcaaa atcaaaaact tcggcgcgat gttcaataaa
     8041 agcataccgg acgacggcat cgccataaat ttgtgcctgg tggacaatac gcgaggcgct
     8101 aacccttgcg cgatcataaa tttggagcag gagttgatgg tcgggcgtta agccttgtgc
     8161 ggcgacgatc atagaatgtt gatcaattaa ggcgtgacca aatattctac attgcccgta
     8221 aactaatgag tcatgaatgg tgacactgtc acttatatat gcaccctgac tgatttcact
     8281 attatcgatc cagacgttat ccgttgcgta aacctctccc cacagcacgc tggtgccggt
     8341 gatacgcgta ttaccagaaa tcaccgttcc gccgaacgca atagcattct ggtcgtaaat
     8401 ccagcaattt ccttcctatg ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc
     8461 tgctatcaca tcgttaaaat cgctaatggc gataatctgc cgtaataaca cacttttttt
     8521 agtgccatct tcctgataac taaaggctcg ctgttcttca cttagacgat atttgcgcat
     8581 agcgttttct cacaggtgac ttactataac cgtagcaaat tctgcgactc tggctatgct
     8641 cgagaaaatt ccataaaatg catttcaaat atactttata aattaaacaa aatgagtaag
     8701 aagatgcaga gcgataaagt gctcaatttg ccggcaggct actttggtat tgtgttgggg
     8761 acgataggga tgggatttgc ctggcgctat gccagccagg tttggcaggt cagccactgg
     8821 ttaggggatg ggctggtgat tctggcgatg atcatctggg gattattaac tagcgcattt
     8881 attacccgac tcatacgctt tccgcatagc gtgctggcgg aagttcgcca tccagtgctg
     8941 agcagttttg tgagtttgtt tccggcaacg acgatgctgg tggcgattgg ttttgttccg
     9001 tggtttcgcc cactggcggt gtgcctgttc agttttggtg tcgtggttca gttggcttat
     9061 gccgcctggc aaactgcggg attatggcgc ggatctcacc ctgaagaagc taccatgcct
     9121 ggactgtatc tgccgacagt tgccaacaac tttatcagcg caatggcctg tggtgcgttg
     9181 ggctacaccg acgccggtct ggtgttttta ggcgcaggcg ttttctcatg gctaagcctt
     9241 gaaccggtga tcttgcagcg tctgcgcagt tcgggagaat tacccacggc actgcggaca
     9301 tcactcggca ttcagctcgc tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac
     9361 ggcggcgagg gtgatacgct ggcgaaaatg ctttttggtt atggactgct gcaactgctg
     9421 tttatgctac gtctgatgcc atggtatctc tcccagccat ttaatgcttc attctggagt
     9481 ttctcgttcg gcgtatctgc actggcaacc accggtttgc atctggggag tggcagcgaa
     9541 aatggatttt tccatacgct ggcggtaccg ctgtttatct ttaccaattt tattattgca
     9601 atactgctca tccgtacttt tgcgcttctg atgcagggaa aattgttagt cagaaccgag
     9661 cgcgctgttt taatgaaagc agaggacaaa gaatgatcat tcgtgacgaa aactatttta
     9721 ctgataaata tgaattaacc cgcacacact ctgaagtact ggaagcggtg aaagtggtta
     9781 aaccgggtaa aacgctggat ctgggctgtg gcaatggtcg taacagtctt tacctggcag
     9841 ccaatggtta tgatgttgac gcatgggata aaaatgccat gagtatcgcc aatgtagagc
     9901 gcattaaatc cattgaaaat ctggataatt tacacacccg agtcgttgat ctgaataacc
     9961 tcacatttga tagacagtac gattttattc tttcgactgt ggtactgatg ttccttgagg
    10021 ctaaaaccat cccagggctg attgccaata tgcaacgttg cactaaactt ggtggttaca
    10081 acctgattgt ggcggcgatg gataccgctg attatccatg taccgtcggc ttcccgtttg
    10141 ccttcaaaga gggagaatta cgtcgatatt acgaaggctg ggagagggtg aaatacaatg
    10201 aagacgtcgg cgagctgcac cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg
    10261 ccacgatgct ggcacgtaaa aaatgacccg ctaagaataa ggcgcgtgaa aattccccac
    10321 gctgagatga tttactgttc ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa
    10381 attaatcaca gtagtcgaag ttttttagca gcttaactta ctgaaattta actactgatg
    10441 attgacttag cccctttttc ggcattgact atgtcgtctg aaaaggggct gaaaaattta
    10501 ttttcaccaa cacttttttt gccacaacac gaagcggcgc tttttgctat aacttaaaaa
    10561 gtaatataat catctcagga aactattcat gcgtaccaca tcatttgcga aagttgcagc
    10621 tttatgcggc ttgttggctc tgtctggttg tgcctctaaa atcacccagc cagataaata
    10681 ttctggttt
//
LOCUS       DAJVOY010000139        10659 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVOY010000139 DAJVOY010000000
VERSION     DAJVOY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.139"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(230..2089)
                     /gene="gss"
                     /locus_tag="OQL90_003112"
     CDS             complement(230..2089)
                     /gene="gss"
                     /locus_tag="OQL90_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1369791.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2294..3160
                     /gene="yghU"
                     /locus_tag="OQL90_003113"
     CDS             2294..3160
                     /gene="yghU"
                     /locus_tag="OQL90_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1369792.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3283..3531)
                     /gene="hybG"
                     /locus_tag="OQL90_003114"
     CDS             complement(3283..3531)
                     /gene="hybG"
                     /locus_tag="OQL90_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1369793.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3544..3885)
                     /gene="hypA"
                     /locus_tag="OQL90_003115"
     CDS             complement(3544..3885)
                     /gene="hypA"
                     /locus_tag="OQL90_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1369794.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3878..4366)
                     /gene="hybE"
                     /locus_tag="OQL90_003116"
     CDS             complement(3878..4366)
                     /gene="hybE"
                     /locus_tag="OQL90_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1369795.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4359..4853)
                     /locus_tag="OQL90_003117"
     CDS             complement(4359..4853)
                     /locus_tag="OQL90_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1369796.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4853..6556)
                     /gene="hybC"
                     /locus_tag="OQL90_003118"
     CDS             complement(4853..6556)
                     /gene="hybC"
                     /locus_tag="OQL90_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1369797.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6553..7731)
                     /gene="hybB"
                     /locus_tag="OQL90_003119"
     CDS             complement(6553..7731)
                     /gene="hybB"
                     /locus_tag="OQL90_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1369798.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7721..8707)
                     /gene="hybA"
                     /locus_tag="OQL90_003120"
     CDS             complement(7721..8707)
                     /gene="hybA"
                     /locus_tag="OQL90_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1369799.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8710..9828)
                     /gene="hybO"
                     /locus_tag="OQL90_003121"
     CDS             complement(8710..9828)
                     /gene="hybO"
                     /locus_tag="OQL90_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1369800.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10017..10304)
                     /locus_tag="OQL90_003122"
     CDS             complement(10017..10304)
                     /locus_tag="OQL90_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1369801.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10423..>10659)
                     /locus_tag="OQL90_003123"
     CDS             complement(10423..>10659)
                     /locus_tag="OQL90_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1369802.1"
                     /translation="VAKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPG
                     VNHGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
BASE COUNT         2599 a         2918 c         2640 g         2502 t
ORIGIN      
        1 ggcaaagtga aatatcattt tttaacgata aaaagagtgt tatttcttgt gtatcagaca
       61 ataagcattc attcaaaacc tttagaaata atcaaaacac aggtattcca ctggttaata
      121 tattcacata tgaaatgaat aaatattaga acatataaat attgaatatt ttgattaatc
      181 cccgtactga ttattcttca taatcagtac ggggttgcta caacatgtat tactttttca
      241 ccacaattaa cggttcaata tcactctctt ttttgatgac cagtgattca tcaccgcgca
      301 aacacgtccc accgtagttg ccgcccacgg tgaaggtaca tacctgaatg tatttaccgt
      361 ccactttcgg caaacaccac agttgctgat agatgttttt ctgctcggca aatttaccgc
      421 tggttttgtc cagcacctct tcatgatggc tgaccaggtc gatattgcta ccacagcgac
      481 cggcaatcgg tttcactgcg taacctgttt tcaccaattc atcattaaca gtgaaatcgg
      541 tatccagcag gtaacgatgg tgcgggaaca gcgaccagag gatcggcaga atcgctttgt
      601 tgccggggat caccgtccac agcggctcaa agactagcac ttccgggcgc agcaatacgt
      661 cgataagacg cacttcgttt tgcggatgac cggtacggat tggcaccgca gcaaactcac
      721 ggtcgctaac ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt ttccacacgc
      781 agttaaccag tcgcccttcc ccatcaatca gttgcccggc agcatcccag cccagttcat
      841 ccaatccacg caagatacgc gtttcaaagc ccgcctggtg cagcgcctgc tccataaact
      901 gcgcgtgata gttttcctcg atatctttgt cctgcatgat atggacaaac ggacgtgcac
      961 gactgtgttt ccaggcacca gccaattcgt taatcagccc ttccgccgga ttgaagccgt
     1021 tgcctttata gccctgctcc gcccaacgtt cgaggatcaa gcccgcttcg gtatgacagg
     1081 aggcggagtc ggcgttgtac tcgtaaacct tcaggccacg ctcatccatg cagaaatcca
     1141 tacgaccagt gatcatatgg tgacggcgac gctgccagga gagacgcaaa cgtggccaga
     1201 ggattttcgg aatgtcgaac agcgccagca ggttgtcatc tttcagtacc ttgtcggttg
     1261 cgtgaagata catcaggtgc agctcgttgg tggctttaat tagctcctgc tcggcactct
     1321 cggtaatggt gtagtaatga taaggatcct gattgatcac ctgaccgttg gcctgcacat
     1381 aggcgttttg cagcggatct ttttcatcca gccatttacc gtcaaactgg cctttgtttt
     1441 ccaggcgcgc tccgctgatt ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt
     1501 attcagtatc ttccgtctgg atcatccagc ccagaatggt ggtgtcatca aaagtgtctt
     1561 tcagggtata gcagccgttt tcgaccacca tctccagctc acgcgtccac tgttgccctt
     1621 gcggcaacgg ggaatgaatc acgttctgtt ccgcaatacg gactttgttg ccatgcaatt
     1681 gggtaatgat ggcgacatgg ccagtgtctt taaattcacc gcctttatcc cagataagaa
     1741 gcgcacccgc gaccggcgca cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt
     1801 tgtcattaac cacttctcgc aggaagcgca gcgagaaaat ctcccacgcc atacccacgt
     1861 cagtaaagac cacaccgtaa ttcagaaaga gaaaacggcg agcaaattca acgcattgcc
     1921 acttgtggcc catatattcg tcgtcgatat agctacggaa tacggcgtca tcttcgtatt
     1981 cctgcggatc gagagaactg taatctgaag agtagattgc taccccacct ggggcgtagc
     2041 ccaataatgt cccgaacggg gcatcctggc tggtcgttcc tttgctcatc actttacctt
     2101 taacaataca acctaagcag ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca
     2161 ttaaccggac agaggtcaga ctaatcatac acctcatcgc agcggctggc gcaaagggtt
     2221 aaaaaattca cattctgtac agcaagtgac ctgccacact gcttcaacac taccactcag
     2281 aaggcaactc actatgacag acaatactta tcagcccgcg aaagtctgga cgtgggataa
     2341 atccgctggc ggcgcgttcg ccaatatcaa tcgcccggtt tctggtccga cgcatgaaaa
     2401 aacgctgccc gttggcaaac acccattgca actttattcg ctgggaacgc cgaacggtca
     2461 gaaagtaacg attatgcttg aggagctgct ggcgctgggc gttactggtg cagagtacga
     2521 cgcctggctg attcgtattg gcgatggcga tcaattctcc agcggctttg tcgaagtgaa
     2581 cccaaactcg aagatcccgg cgctgcgcga tcatacgcat aatccgccga tctgtgtgtt
     2641 tgaatctggt tcgatcctgc tttatctggc ggagaaattt ggctacttcc tgccgcagga
     2701 tttggcaaag cgtactgaaa cgatgaactg gctgttctgg ttacagggcg cggcaccgtt
     2761 cctcggcggt ggttttggtc acttttacca ttacgcaccg gtaaagattg agtacgccat
     2821 caaccgcttt accatggaag ccaaacgtct gctcgacgtg ctggataagc aactggcgca
     2881 gcataagttt gttgcgggcg atgagtacac cattgcggat atggcgattt ggccgtggtt
     2941 tggcaacgtg gtgttaggtg gtgtgtatga tgccgctgag tttcttgatg cgggcagtta
     3001 taagcatgta caacgctggg cgaaagaagt aggcgaacgt ccggcggtga aacgtgggcg
     3061 tattgttaac cgcaccaacg gaccgctgaa tgagcagttg catgagcgcc atgacgccag
     3121 tgatttcgag acgaatacgg aagataagcg tcaggggtaa gggttggtgt tcgtcgcagc
     3181 aagccatcca ggccggataa ggcgttcacg ccgcatccgg caatcgtgca caatgcctga
     3241 tgcgacgctg ccgcgtctta tcaggcctac gaaagctaat catcacgcgc tggtaatgtc
     3301 gtaatccatt tggcgcagtg cgtctaatgt ggctttggct tcatcttcgt cgatgatgct
     3361 catggcaaat ccgacgtgta ccagcaccca ctggcccagt agatcggcag ggttaccttc
     3421 acaaatcagg gcgatattca cgtcgcgctt gataccacat acttcaacct gcgcaagctg
     3481 gtgaatatct tcaccgacag ccagcacctg gcctggaacg ccaatacaca ttattaactc
     3541 cggttattca acttcaatac ttttgacgat cagcgaatcg ccggtatcga cacgcaaccg
     3601 ctcgccgtga cagagcggac actgcgcatc gtgctgatga atctccacca cctggctgca
     3661 atcccagcac caggcctggg cgggtttata gacgatatgt aaatcgcacc cttgcgccac
     3721 cgttccgtgg cagacaattt caaaactaaa acggacggcg ctctcctcaa cgcaggagag
     3781 cgcgccaatt tccagccaca cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg
     3841 ttggataatt tcaacggcgc tctggcaaag cgacaactca tgcattttcg ccactcctgc
     3901 gaccaaacag cagggcgcga cgccctgcgt gtggtacatc cggattcgtg actggcagcg
     3961 aaaggatcat tcgtgcgcag tcatccgtca ggcgttggcc ctcttcaatc gacatactgt
     4021 gcgaaagcgg tgacatcagc gaacaggaga ggtattgcga aacaccgtcc agttcgccaa
     4081 cggtaaaggt catggtgcca tacggcagtt gcagaccaat tttttcactc actttgcgca
     4141 gcggccagag ttgatccggg ccggggaaga taactgcact cagcatccac ggggtgatca
     4201 cacaccccgt ccactgacct tcgaacagcg taaaatcaga aacatacacc ggcattgaag
     4261 gatgcagaaa agagagatcg tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta
     4321 cttgcgcctt cggggaggtc tggaaacctg ctatctcttc agtcatgaat cgcctcccgt
     4381 gggatggctt ccacgcctga ttcgcgcagc gcagccagaa cctgctcaag cgcaggttca
     4441 atcattgctt caaccgtcgg cgttaagccg atgtgtggct ccagcgattc cgggatcacg
     4501 ccgaccaggg tcagcttttt cggaaactca ccggtgaagc gcagggccga caatacgtcg
     4561 gccaggccaa gctgatgcgg agagattttg ttggtaaaca acgccggaac ttcttcatcc
     4621 cgcaggatca tcatcgttcc cggcgtgttc tttttcgaca caatggcatc cgcaataatc
     4681 agatgatcgc gatttgccat gtcgccaagc agctccattc ccgccgtgcc gccatcgagg
     4741 atctcaacat aatccggcag aatgtatcgt tgctctaacg cttcgacaat ccgcacaccg
     4801 atggcttcat cggtcagcaa aatattgccg acccctaaga ctaaaatacg cattacagaa
     4861 ccttcactga aaccacttcg ttgccgtcgg catccactac gtgtaccgca caggccatgc
     4921 acgggtcgaa ggagtgaatg gtacgcacca cttccagcgg tttattcgga tcagcaaccg
     4981 gtgtacccac cagcgactgc tcgtaaggac cgacgtcatc attgaagtta cgcggaccag
     5041 agttccaggt tgatggaaca actgcctggt agttactgat aataccgtct ttaataacca
     5101 tccagtgaga gagcataccg cgcggcgctt cgaggaagcc aacacctttg aactcacccg
     5161 ttgccggaat gttcggtttc acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca
     5221 gtgcactgta ttgattttgc aggatatcct gcaattcgca gcagtgaacg gtacgaccaa
     5281 taatacggcc cagcgtggag tgcagctgcg ccacttccag cgtgttgcca gtcagtttct
     5341 gataaatcgc aacgatttca ttcagtttgt tctgggtaga ttcgcgacct gccgccagtt
     5401 tcaccagcat attagccagc ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt
     5461 tcacccagga atatttcccg tcgtcagacc aaccatcata agccggaatg gtggtacctt
     5521 cccacggtgc ctgcggcgct tcgtctttat accaggagtg cttcgcgctt tcctgaatgc
     5581 ctttgatcag gtattcatcg gaatgagaag tgatcggacg atacgaggac agatccgcat
     5641 tctcaatgta gccgcccggg aacaggaagc tgccgttttt actgtcggtc gggaattccg
     5701 gcacgctcag gtagttcacc gcacctttac cgcgcgtcag ccattccggg tagaacgcgg
     5761 caataactgc ggtatcaacc ttataaacct gttcaacaaa gtcgctcagc ttgtcgatga
     5821 aagacttgat gtacatcagg cgctcaaggt tcagcacgcc caaaccgtcg aggttgatcg
     5881 ggttcgcgac accacctacc gccaggttct gaatgtgcgg cgttttaccg cccagcagcg
     5941 ccacgacgcg gttagcgtca cgctggcact ccagcgcctg caggtagtgc gctaccgcaa
     6001 tcaggttcac ttccggcggc agtttcatcg ccgggtgacc ccagtagcca ttggcgaaaa
     6061 tacccaactg accgctggca accagatctt tgatcttgtt ctgaacttta gtgaactctt
     6121 ccggactgtt cagatgccag gtcgaaacgc ctttcagcat ttcggaggct ttggttgggt
     6181 cagcttgcag tgcagaagtg atgtccaccc agtccagcgc cgaaagctga tagaaatgaa
     6241 cgatatggtc atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa
     6301 ccggaacgtc gatattcagc gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg
     6361 tagtacatac gccacagata cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt
     6421 tcacgatctc ttccatgccg cgccacatgg taccggaagc ccatgctttc gaaacgacgc
     6481 cattttcgat ttcgcaatcg atgcgtaaat gcccctcaat acgggttacc ggatcaatag
     6541 taattctctg gctcatgctt tgctcgcctc atgacgatta tgatcgtttt gttttaaagg
     6601 aggaagtatc ggcagtagac gaatgagtac gatgtaagcg caaatctcaa tagccacaaa
     6661 accaatagaa atcaacagtt cttccctggt cgggaagtag gcgtaaccgc cgcccgggtt
     6721 gaatgccacc agcgaatagg tcagacgcca ggttgcacaa cctaacagtg cgctcagtgc
     6781 tgacaggaac agcatgcggg aatcattacg cagcttcgcc acacgcagaa cgaccagcgg
     6841 gaagagcatc agcaggactt caatccagaa catcacggag tagaagtcac cggcaaacgc
     6901 taacgacagc ttgtcgcgat agataagctc gccaaagcgc agcacgataa aaatcgccag
     6961 caacacactg atggtgttgg tcagcttgac gaacagactc ttttcatccg gaccgttgcc
     7021 acgcagaccc gcctgcacca gcgaaccttc aaagatgaca atcgagaagc ccatgatgaa
     7081 cgccgtcagc agcgagaaca gcggcaacat ttcatagctc tgccacaacg gatgcacctt
     7141 gtagcccgcc gagatcatca gtgaccccat tgaagactgg tgcatggtcg gcaacagcgc
     7201 gccaagtgca atgatgaaga acatcacctt gttcagacgc ttgagagaaa ccttccagcc
     7261 cagacgttca aacagtgccg gagcaaactc cagtgccatc acgccgatat agatggtcat
     7321 acagaccgcc gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa tgtagaagta
     7381 cggcaggttc cagtagcgac ccacgtcgat agtgatcgac aagccaccca gtgagtaacc
     7441 aaacagactc gccaacagcg ccggacgcac cagcggatgg tattgcccac ggttaaagac
     7501 gtataccgcc cacgccagcg cccagccgcc acaggcaaag ccggtaccaa tcagcaggtc
     7561 aaacgcgatc cacacgcccc acgggaagcc gccgttcagg tcagagacag agcccagacc
     7621 gaacaccaga cgcttcacaa tcaggagcat acagatgacg attaacggtc caaaaatcat
     7681 gaccggttta ctgatgattt tgccgcccag cggttgtgga tcatgactca tgatcgtctc
     7741 ctccgtcgtg atggtcgttt ttggtgttgc gacgaaccag cacggttaag cccgccagca
     7801 cagccagtgg tagcatcatg cctttataca gggtgtgttg aatattttcg gaacgcgcac
     7861 cggtagaaag atcgtccagt ttcggcaggt cgagattttc ataaggcaca cccgtcagta
     7921 ccagaacctg agtaccgccg ccctctttct cgccgtacag atgcggataa tatttcggca
     7981 ccgtatgcag gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg tattcgctgc
     8041 caggcttcag cgccagacgt tttttcgcct ccgccatcag ctcttcacgc gtaccgaaaa
     8101 tcaccgcgcc cgccgggcac acttctacgc agccaggcag accgccttta tcgagacgtt
     8161 ccacaccttt ctggttgcac agctcgcact tatgcagcgc accaaacggg ttgttgtagt
     8221 cgtacttcgg cacgttgtac ggacaggcga ccatgcagta acggcaaccg gtgcacacgt
     8281 ctttgtcgta atggacaatg ccggttttcg gatctttttt cagtgcagag accgggcaca
     8341 cagagacaca gttcggatcg acgcagtgca tacactgttt cttaatgtac gcgtagccgt
     8401 tctcctcctg gtctttgttg acccctgtgc cgctggtcca cacctgaatg atgttattgg
     8461 tatacggcga cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg ttacgttcag
     8521 ggaagttgat atcctgacac ttggtgacgc aagcctggca gcctacgcac aaggtcgagt
     8581 cgtacaacat ccccagcgat cccggaattg gcgggcggtt ttcagcagcc gcatgactga
     8641 cagacggcag cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata aaattacgtc
     8701 tgttcacggt tattctcccc gtgagtcagc gttatctttc ttttgctgac gacccagttc
     8761 acgcaccgcc atcacgctga caccggcaac cagcccaacc acaccgccga gcaaaccaat
     8821 agcgcctgca gagacgttac cgccctcttt agcgttaaca tccggtttct gtgaacgcgg
     8881 agtctgattt tcgacgttgg caagctgatg gatgccttta tggaagccga taccttcttc
     8941 gttacagcca tagcatgggt gaccaatcgc taccggccac acgccgccaa catcgcagaa
     9001 ttgcagcgtt gagcagttgc cgtaagtttc tggcccttta cagccgaggt ggtacaggca
     9061 ccagccttca cggtggcctt catcaccgaa ctctttggca aaacgaccag catcgaagtg
     9121 cgggcgacgt tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg gacgattttt
     9181 gtcatccagt ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt
     9241 gtgcgggttc ggcgggcagc ccggaatatt gataacggtt ttgcctggca gaacttcttg
     9301 caggctgact gcgccagttg ggttaactcc agctgcggca acaccgcccc acgcagagca
     9361 ggaaccgata gcgataatcg ctgctgcgcc ttctgccgct ttgcggatgt gatccacaat
     9421 cggctcacca gcaaccatgc aataaatacc gttatctttt aatgggatgg aaccatccac
     9481 aaccaacaca tactgccctt tgtacttctc aagtgcgtta tgtttgttct cttcgacctg
     9541 atgaccgaag gcggcggaaa gcacttcgtg atactccaga gagatagtct ccagcacgag
     9601 gttttctacc gttggatgcg ttgcacgaag cagagattcc gtacaaccgg tgcactcctg
     9661 cgcgccaatc cagataactg gcggacgctg cgggttagta accgattcgg ccatctctgc
     9721 agcggctttg ctacttaacc ccatggtggc ggctaatgct gcacaaagct tcatgaaatc
     9781 acgacggtta atgccgtgag aatggatgag ggtgttatct ccagtcattt atagttattc
     9841 cgttgcgaag acctggcata tattttgcct caatcgcaaa atcaataatg cgatcgatgc
     9901 gccatttacc acacatttat tatggttatc gatatcatga tactgcggcg gggcgctaaa
     9961 acgaagggaa gaagcattag tgtaattaat taaagcaaga taatacgtat gtttgatcaa
    10021 ttttcgaggc gataaccggc cataaaaaag cgcaacgtag aacaggaatt attctcacga
    10081 aagaaatcca ttaccatctc tttatccagt ccatagcggc gcgtcagaat acccgcttcc
    10141 caggcgctaa gctggcgatc tccggtcttt tcgtacatcc ggtgtgagta gcctaataca
    10201 aaaccgcgtt tataatcggc acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt
    10261 gcttttaatc ccgccattaa gcctttacca aaatggttat tcatcgttca cctcattcgc
    10321 tatatattat catatatagc aatattttag gcaaagaacg acttttgcgc acgctatttt
    10381 ttcatgcttg tttaagacgt aatcccgtaa gagataaacc catcaggaga gatatttatc
    10441 gaaccatttc agcgtccttt gccaggcaag atcggcggca gatttgtcat aacgaggcgt
    10501 ggaatcatta tggaatccgt gattaacccc cggatagata tacgcctcat agaccttatt
    10561 attggctttc aacgccgcct cataagcggg ccagccctcg ttgattcggg tgtccagttc
    10621 cgcgaagtgg agtagtaaag gcgcttcaat cttcgccac
//
LOCUS       DAJVOY010000140        10630 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVOY010000140 DAJVOY010000000
VERSION     DAJVOY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.140"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..175
                     /locus_tag="OQL90_003124"
     CDS             <1..175
                     /locus_tag="OQL90_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1369803.1"
                     /translation="IILQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCL
                     HNSCIIKICSICY"
     gene            complement(243..1493)
                     /gene="icd"
                     /locus_tag="OQL90_003125"
     CDS             complement(243..1493)
                     /gene="icd"
                     /locus_tag="OQL90_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1369804.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1665..2318
                     /gene="rluE"
                     /locus_tag="OQL90_003126"
     CDS             1665..2318
                     /gene="rluE"
                     /locus_tag="OQL90_003126"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1369805.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2328..2789
                     /gene="nudJ"
                     /locus_tag="OQL90_003127"
     CDS             2328..2789
                     /gene="nudJ"
                     /locus_tag="OQL90_003127"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1369806.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2843..3949
                     /gene="mnmA"
                     /locus_tag="OQL90_003128"
     CDS             2843..3949
                     /gene="mnmA"
                     /locus_tag="OQL90_003128"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1369807.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3985..4626
                     /gene="hflD"
                     /locus_tag="OQL90_003129"
     CDS             3985..4626
                     /gene="hflD"
                     /locus_tag="OQL90_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1369808.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4630..6000
                     /gene="purB"
                     /locus_tag="OQL90_003130"
     CDS             4630..6000
                     /gene="purB"
                     /locus_tag="OQL90_003130"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1369809.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6168..6839
                     /gene="phoP"
                     /locus_tag="OQL90_003131"
     CDS             6168..6839
                     /gene="phoP"
                     /locus_tag="OQL90_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1369810.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6839..8299
                     /gene="phoQ"
                     /locus_tag="OQL90_003132"
     CDS             6839..8299
                     /gene="phoQ"
                     /locus_tag="OQL90_003132"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1369811.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8375..9496
                     /gene="roxA"
                     /locus_tag="OQL90_003133"
                     /gene_synonym="ycfD"
     CDS             8375..9496
                     /gene="roxA"
                     /locus_tag="OQL90_003133"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1369812.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9545..>10630)
                     /gene="pepT"
                     /locus_tag="OQL90_003134"
     CDS             complement(9545..>10630)
                     /gene="pepT"
                     /locus_tag="OQL90_003134"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1369813.1"
                     /translation="INVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVN
                     PQIVENYRGGDIALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIM
                     TALAVLQQKNIPHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFEN
                     FNAASVNIKIVGNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAG
                     MKGTVERADMHYIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMR
                     EKVVEHPHILDIAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGK
                     HEFVTLEGMEKAVQVIVRIAELTAQRK"
BASE COUNT         2618 a         2723 c         2677 g         2612 t
ORIGIN      
        1 tattatcctt caggaaaaag aatatcctat tactatttat aacattgcac cattcaacaa
       61 gaatttatgg agtttactca aaagcagtca agagtgtcct ccaggagaaa gcaaaataac
      121 aaataaatgt ttacataata gttgcattat aaaaatatgc tcgatatgct attaataata
      181 tccacataaa taaaacaacg ggcgtgttat acgcccgttt caatatttaa ctcatggaga
      241 gattacatgt tttcgatgat cgcttcacca aactctgaac atttcagcag cttagcgcct
      301 tccatcagac gttcaaagtc ataagttacg gttttcgcgt tgatagcacc ttccatacct
      361 ttaacaatta aatcagccgc ttcagtccac cccatatggc gcagcatcat ctcagcggag
      421 aggatgatag agcctggatt tactttgtcc tgaccggcgt atttcggcgc agtaccgtgg
      481 gtggcttcaa acagggcgca ttcgtcaccg atgtttgctc caggagcgat accgataccg
      541 ccaacctgcg ctgccagggc gtcagaaatg tagtcaccgt tcaggttcat acaggcgata
      601 acatcatatt cagccggacg cagcaggatc tgttgcagga atgcatcagc aatcacgtct
      661 ttaatgacga tctctttgcc ggtgttcggg tttttaactt tcagccacgg gccgccgtcg
      721 atcagttcac cgccaaactc ttcacgcgcc agctggtagc cccagtcttt aaacgcgcct
      781 tcggtgaact tcatgatgtt gcctttgtgc accagagtca cagagtcacg atcgttagca
      841 attgcgtatt cgatcgctgc acgaaccaga cgtttggtgc cttcttccga acacggctta
      901 ataccgatac cacaatgttc cgggaagcga attttcttca cccccatctc ttcacgcagg
      961 aatttaatca ctttctcggc gtcggcagag tctgctttcc attcgatacc cgcataaatg
     1021 tcttccgagt tttcacggaa gataaccata tcggtcagtt cagggtgttt aaccgggctt
     1081 ggagtgccct gatagtaacg taccggacgc aggcagatgt agagatccag ttcctggcgc
     1141 agggcaacgt tcagagagcg aataccgcca ccaacaggag tggtcagcgg acctttaatg
     1201 gcaacgcgat attcacgaat cagatcaagg gtttcagcag gcagccagac atcctgacca
     1261 taaacctgtg tggatttttc accggtgtaa atttccatcc aggagatttt acgctcgcct
     1321 ttataggctt tctcgactgc agcgtcgacc actttcagca tggctggggt tacatctaca
     1381 ccgattccat caccttcaat gtaagggata atcggatttt caggaacgtt gagtttgccg
     1441 ttttgcaggg tgatcttctt gccttgtgcc ggaacaacta ctttactttc cattcacctc
     1501 tccttcgagc gctactggtt tgctcgtctg ccaccacgtt gcatatgcgt ttgcgtcctg
     1561 cgatacggat gctttagagc aattttttgt taatgatttg taattggctt gtcaatacta
     1621 ccctattgtt tgccgtcatg aaagatgcgc gttattaggc tataatgcgg caattcataa
     1681 tctctgaaaa taccatgcaa aaaacttctt ttagaaatca cctggttaag cgattcagct
     1741 cgcaacgttc taccaggcgt aaacctgaaa accagcccac gcgtgtgatc ctgttcaata
     1801 aaccctacga tgttcttccg cagttcaccg atgaagccgg acgcaaaaca ttaaaagaat
     1861 tcatcccggt tcagggcgtt tatgcagcag gtcgtcttga ccgcgatagc gaagggttac
     1921 tggtgctgac caataacggc gcgttgcagg cgcgtttaac ccagccgggc aaacgcaccg
     1981 ggaaaatcta ttatgtgcag gtggaaggta ttcccacaca agacgcactt gaagccttgc
     2041 gcaatggcgt aaccttaaat gatggcccta ccctgcccgc cggcgcggag ctggttgacg
     2101 aaccagcgtg gttatggccg cggaatccac caattcgtga acgcaaaagt attcccacca
     2161 gctggctgaa gatcacctta tatgaaggac gtaatcgcca ggtgcgccgc atgaccgccc
     2221 atgttggttt ccccacgctg cgactgattc gttatgcgat gggtgattac actttggata
     2281 accttgccaa tggcgaatgg cgagaagcaa cagaataagg atgcacaatg tttaaaccac
     2341 acgttaccgt tgcttgcgta gtgcacgcag agggtaaatt tttagtcgtt gaagagacga
     2401 ttaatggtaa agcgttatgg aaccaacctg ccgggcatct ggaagccgat gaaaccttag
     2461 tggaagctgc cgcccgtgag ctatgggaag aaaccggcat cagcgcgcaa ccgcaacact
     2521 ttattcgtat gcatcagtgg attgcgccag ataaaacgcc gtttttgcgt ttcctctttg
     2581 ccattgagct tgagcaaata tgcccgaccc agcctcatga cagcgatatc gactgctgtc
     2641 gttgggtcag cgccgaagaa attttaaagg cgtcaaatct tcgttcgccg ctggtggcgg
     2701 aaagtatccg ttgttatcaa agcgggcaac gctatccgct ggagatgatt ggcgatttta
     2761 actggccttt tacaaagggt gtcatctaaa aggctgcgtg atagaatacg ccgccttgaa
     2821 gttcaatgtc gtgagtgatc caatgtctga aaccgcaaaa aaagtaatcg tcggcatgtc
     2881 cggcggtgtc gattcctccg tttctgcctg gctgttgcaa caacagggat atcaggttga
     2941 aggcctgttt atgaagaact gggaagaaga cgacggtgag gaatattgca cagcggcagc
     3001 ggatctggct gatgcccaag ctgtctgcga caagctcggc attgaactgc acaccgttaa
     3061 ctttgctgcc gagtactggg acaacgtctt cgaactgttc cttgccgaat ataaagccgg
     3121 tcgcacgccg aatccggata ttctgtgcaa caaagagatc aaatttaaag ctttcctcga
     3181 atttgccgcc gaagatttag gtgccgatta tatcgctacc ggtcattacg tacgtcgggc
     3241 cgatgtcgat ggcaagagcc gcctgctgcg tggtctggac agcaataaag accagagcta
     3301 cttcctttat acgctcagcc atgagcagat tgcgcaaagc ctgttcccgg tcggcgaact
     3361 ggaaaaaccg caggtgcgta agattgctga agatcttggt ctggtcaccg cgaagaaaaa
     3421 agactctacc ggcatttgct tcattggcga acgtaaattc cgcgagttcc tgggccgtta
     3481 tctcccggcg caaccgggca aaatcattac cgtcgatggc gatgaaattg gtgagcacca
     3541 ggggctgatg tatcacactc tcggtcagcg taaaggtctg ggtatcggtg gcaccaaaga
     3601 aggcaccgaa gaaccgtggt atgtggtgga caaagacgtc gaaaacaaca ttctggttgt
     3661 tgcgcagggc catgaacacc cgcggctgat gtctgtcggg ttgattgccc agcagttgca
     3721 ctgggtcgat cgcgaaccat tcaccggtac tatgcgttgc acggtaaaaa cccgctatcg
     3781 ccagaccgat atcccttgca ccgtcaaggc gctggacgat gatcgcattg aagtgatttt
     3841 cgatgaaccg gttgccgccg tgacgccggg ccagtctgcc gtcttctata acggtgaagt
     3901 gtgcctcggt ggcggtatta ttgagcagcg tctgccgctg ccggtctgat tattatcttt
     3961 acttaaaaca aggaagcagt gaacgtggca aagaattact atgacatcac cctcgccctg
     4021 gccggtattt gtcagtcggc acgcctggtg caacaactcg ctcaccaggg gcattgtgat
     4081 gccgatgcgc tacacgtctc actcaacagt attattgata tgaaccccag ctcgacgctg
     4141 gcggtttttg gcggtagcga agccaacctg cgcgtcgggc tggaaaccct gctcggcgtg
     4201 ctcaatgcca gcagtcgcca gggcttaaac gccgaattaa cccgctacac actcagcttg
     4261 atggtgcttg agcgcaaact ctcctcagcg aaaggcgcgc tcgacactct gggcaaccgg
     4321 atcaacggcc tgcaacgcca gctcgaacac ttcgatttac agtccgaaac gctgatgagc
     4381 gcgatggctg ctatctatgt tgatgtgatc agcccgcttg gcccgcgcat tcaggtcacc
     4441 ggttcccctg ctgtactgca aagcccacaa gtgccggcga aagttcgcgc aaccctgctg
     4501 gcaggcattc gcgccgccgt gctctggcac caggtcggcg gcggacgtct gcaactgatg
     4561 ttttctcgta atcgcctgac cactcaggca aaacaaattc ttgctcattt aaccccggag
     4621 ttgtgatcta tggaattatc ctcactgacc gccgtttccc ctgtcgatgg acgctacggc
     4681 gataaagtca gcgcgctgcg cgggattttc agcgaatatg gtttgctgaa attccgtgta
     4741 caagttgaag tacgttggct gcaaaaactg gccgcgcacg cagcgatcaa ggaagttcct
     4801 gcttttgctg ccgacgcaat cggttacctt gatgcaattg tcgccaattt cagcgaagaa
     4861 gatgccgcac gcatcaaaac catcgagcgt accactaacc acgacgttaa agcggttgag
     4921 tatttcctga aagaaaaagt ggcggagatc ccggaactgc acgcggtttc tgaattcatc
     4981 cactttgcct gtacttcgga agatatcaat aacctctccc acgcattaat gctgaaaacc
     5041 gcgcgtgatg aagtgatcct gccgtactgg cgtcaactga ttgatggcat taaagatctc
     5101 gccgttcagt accgcgatat cccgctgctg tctcgtaccc acggtcagcc agccacgccg
     5161 tcaaccatcg gtaaagagat ggcaaacgtc gcctaccgta tggagcgcca gtaccgccag
     5221 cttaaccagg tggagatcct cggcaaaatc aacggcgcgg tcggtaacta taacgcccac
     5281 atcgccgctt acccggaagt tgactggcat cagttcagcg aagagttcgt cacctcgctg
     5341 ggtattcagt ggaacccgta cactacccag attgaaccgc acgactacat tgccgaactg
     5401 tttgattgcg ttgcgcgctt caacaccatt ctgatcgact ttgaccgtga cgtctggggt
     5461 tatatcgccc ttaaccactt caaacagaaa accattgctg gtgagattgg ttcttccacc
     5521 atgccgcata aagttaaccc gatcgacttc gagaactccg aagggaatct gggcctttcc
     5581 aacgcggtat tacagcatct ggcaagcaaa ctgccggttt cccgctggca gcgtgacctg
     5641 accgactcca ccgtgctgcg taacctcggc gtgggtatcg gttatgcgct gattgcgtat
     5701 caatccaccc tgaaaggcgt gagcaaactg gaagtgaacc gtgaccatct gctggatgag
     5761 ctggatcaca actgggaagt gctggctgag ccaatccaga cagttatgcg tcgctatggc
     5821 atcgaaaaac cgtacgagaa gctgaaagag ctgactcgtg gtaagcgcgt tgacgccgaa
     5881 ggcatgaagc agtttatcga cggtctggcg ttgccagaag aagagaaagc ccgcctgaaa
     5941 gcgatgacgc cggcaaacta cattggtcgc gccatcacca tggttgatga gctgaaataa
     6001 actcgtatca gtgccggatg gcgatgctgt ccggcctgct tattaagatt atccgctttt
     6061 tattttttca ctttacctcc cctccccgct ggtttattta atgtttaccc ccataaccac
     6121 ataatcgcgt tacactattt taataattaa gacagggaga aataaaaatg cgcgtactgg
     6181 ttgttgaaga caatgcgttg ttacgtcacc accttaaagt tcagattcag gatgctggtc
     6241 atcaggtcga tgatgcagaa gatgccaaag aagccgatta ttatctcaat gaacatttac
     6301 cggatattgc gattgtcgat ctcggattgc cagacgagga cggtctgtca ctgattcgcc
     6361 gctggcgtag caatgatgtt tcactgccga ttctggtatt aaccgcccgt gaaagctggc
     6421 aggacaaagt cgaagtatta agtgctggtg ctgatgatta cgtgactaaa ccatttcata
     6481 ttgaagaggt gatggcgcga atgcaggcat taatgcggcg taatagcggt ctggcttcac
     6541 aggtcatttc gctacctccg tttcaggttg atctctctcg ccgtgaatta tctattaatg
     6601 acgaagtgat caaactgacc gcgttcgaat acaccattat ggaaacgttg atacgcaata
     6661 atggcaaagt ggtgagcaaa gattcgttaa tgctccaact ctatccggat gcggagctgc
     6721 gggaaagcca taccattgat gtactgatgg gacgtctgcg caaaaaaatt caggcacaat
     6781 atccgcaaga agtgattact accgttcgcg gccagggcta tttgttcgaa ttgcgctgat
     6841 gaaaaaatta ctgcgtcttt ttttcccgct ctcgctgcgg gtacgttttc tgttggcaac
     6901 ggctgcggta gtactggtgc tttcgcttgc ctacggaatg gtcgcgctga tcggttatag
     6961 cgtcagtttc gataaaacta cgtttcggct gttacgtggc gagagcaatc tgttctatac
     7021 ccttgcgaag tgggaaaaca ataagttgca tgtcgagtta cccgaaaata tcgacaagca
     7081 aagccccacc atgacgctaa tttatgatga gaacgggcag cttttatggg cgcaacgtga
     7141 cgtgccctgg ctgatgaaga tgatccagcc tgactggctg aaatcgaatg gttttcatga
     7201 aattgaagcg gatgttaacg ataccagcct cttgctgagt ggagatcatt cgatacagca
     7261 acagttgcag gaagtgcggg aagatgatga cgacgcggag atgacccact cggtggcagt
     7321 aaacgtctac ccggcaacat cgcggatgcc aaaattaacc attgtggtgg tggataccat
     7381 tccggtggag ctaaaaagtt cctatatggt ctggagctgg tttatctatg tgctctcagc
     7441 caatctgctg ttagtgatcc cgctgctgtg ggtcgccgcc tggtggagtt tacgccccat
     7501 cgaagccctg gcaaaagaag tccgcgaact ggaagaacat aaccgcgaat tgctcaatcc
     7561 agccacaacg cgagaactga ccagtctggt acgaaacctg aaccgattgt taaaaagtga
     7621 acgcgaacgt tacgacaaat accgtacgac gctcaccgac ctgacccata gtctgaaaac
     7681 gccactggcg gtgctgcaaa gtacgctgcg ttctctgcgt agtgaaaaga tgagcgtcag
     7741 tgatgctgag ccggtaatgc tggagcaaat cagccgcatt tcacagcaaa ttggctacta
     7801 cctgcatcgt gccagtatgc gcggcgggac attgctcagc cgcgagctgc atccggtcgc
     7861 cccactgctg gacaatctca cctcagcgct gaacaaagtg tatcaacgca aaggggtcaa
     7921 tatctctctc gatatttcgc cagagatcag ctttgtcggt gagcagaacg attttgtcga
     7981 ggtgatgggc aacgtgctgg ataatgcctg taaatattgc ctcgagtttg tcgaaatttc
     8041 tgcaaggcaa accgacgagc atctctatat tgtggtcgag gatgatggcc ccggtattcc
     8101 attaagcaag cgagaggtca ttttcgaccg tggtcaacgg gttgatactt tacgccctgg
     8161 gcaaggtgta gggctggcgg tagcccgcga aatcaccgag caatatgagg gtaaaatcgt
     8221 cgccggagag agcatgctgg gcggtgcgcg gatggaggtg atttttggtc gccagcattc
     8281 tgcgccgaaa gatgaataaa tatgtccata cttcacgcat tacgttaagc atccgttata
     8341 atcggttgca gataccagcc tgtggatgct taacatggaa taccaactca ctcttaactg
     8401 gcccgatttt cttgaacgtc actggcagaa acgcccggtg gtgttaaaac gcggctttaa
     8461 taattttatt gacccgatct ctccagacga gttggcgggt ctggcgatgg aaagcgaagt
     8521 tgacagtcga ctggtcagtc accaggatgg caaatggcag gtcagccacg gcccgttcga
     8581 aagctacgat catctcggtg aaaccaactg gtcattactg gtacaggcag tgaaccactg
     8641 gcatgagccg accgccgcgc tgatgcgacc gttccgtgaa ctaccggact ggcgtattga
     8701 tgatctgatg atttcttttt ctgtacctgg cggcggcgtc ggcccgcatc tcgatcagta
     8761 cgacgtgttt atcattcagg gtaccggacg tcgtcgctgg cgagtgggcg aaaagctgca
     8821 aatgaaacag cactgcccac atccggatct gttacaggtc gatccgttcg aagccatcat
     8881 cgatgaagag ctggagcctg gtgatattct ttatattccg ccaggattcc cgcatgaagg
     8941 ctacgcgctg gaaaatgcga tgaactattc cgtgggcttt cgcgcgccaa atacgcggga
     9001 actgattagt ggatttgccg attatgtgct gcaacgtgaa ctgggcggca actactacag
     9061 cgatcctgat gttccacctc gcgctcatcc tgcggatgtt ctgcctcaag agatggataa
     9121 actgcgtgag atgatgctcg aattgatcaa ccagccggaa cactttaagc aatggtttgg
     9181 cgagtttata tcccagtcac gtcatgaact ggatatcgcg ccgccggaac cgccttatca
     9241 gccagatgaa atctacgatg cgctgaaaca aggtgatgtg ctggtgcgcc tgggtggtct
     9301 gcgcgtattg cgcattggcg acgacgtgta tgccaatggt gagaagatcg attccccgca
     9361 ccgtccggca ctggatgcac tcgccagcaa cattgcgctg actgcggaga attttggcga
     9421 tgcgctggaa gatccgtcat tcctcgcgat gctcgcggcg ctggtcaata gcgggtattg
     9481 gttcttcgaa gggtaagttt ggatttaagc cggatgcggc atcacatgcc gcatcccttt
     9541 tcgcttactt ccgttgcgcc gttaactcgg caatacggac gatcacctgc accgcttttt
     9601 ccataccttc cagagtcaca aactcatgct taccatgata gttgtaaccg ccagtgaaca
     9661 agttcgggca cggtaatccc ataaacgaca actgcgcgcc gtcggtaccg ccgcggatcg
     9721 gtttcagttc cggttcaata tcgcagtcac gcattgcctg ctgggcgata tcgagaatat
     9781 gcggatgctc aaccactttc tcgcgcatat tgtagtaact gtcttcaatc accagttcaa
     9841 tgtagcaatc aggatgtaac cctttgccca cttttttggc gatctccatc attttacatt
     9901 tacgcgcttc aaactgttta cggtcgaaat cacggatgat gtagtgcata tcggcccgtt
     9961 caacggtgcc tttcatgccc gccaggtgat agaaaccttc atagccttct gtcatttccg
    10021 ggctttcatc cgccggaact tccgcatgaa tacgtgccgc cagcgacagc gcatttacca
    10081 tcactccttt cgccgtgccc ggatgaacat tgttaccgac aattttgata ttgaccgatg
    10141 cggcgttgaa gttttcaaac tccagttcgc ctacgccacc accgtcaaca gtgtaagccc
    10201 agcgggcatc gaaggcatca acatcaaaat gtttcgcccc tttgcccact tcttcatccg
    10261 gggtaaaggc gacgcgaata tcaccatgcg gaatgttttt ctgttgcaat accgccagcg
    10321 cggtcatgat ttctgcaata cctgctttgt catcggcacc taacaaggtt ttaccatcgg
    10381 tggtaatcaa tgtctgaccc agtagctgat gcagcaccgg gaacataacc ggtgataaaa
    10441 cttcatcgcc gatacccagc gcaatatcgc caccgcgata gttttcaaca atttgcggat
    10501 taacattttt gccgctgcaa tccggtgagg tatccacatg agaaataaag ccaatcgccg
    10561 ggatatcgcc agggacgtta gccggtaacg tcgccatcaa agtgcccttc tcacttaagg
    10621 tcacattgat
//
LOCUS       DAJVOY010000141        10605 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVOY010000141 DAJVOY010000000
VERSION     DAJVOY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.141"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="OQL90_003135"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="OQL90_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1369814.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="OQL90_003136"
     CDS             complement(1606..1929)
                     /locus_tag="OQL90_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1369815.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OQL90_003137"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OQL90_003137"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1369816.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OQL90_003138"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OQL90_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1369817.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLHGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OQL90_003139"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OQL90_003139"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1369818.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OQL90_003140"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OQL90_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1369819.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="OQL90_003141"
     CDS             7529..8566
                     /locus_tag="OQL90_003141"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1369820.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OQL90_003142"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OQL90_003142"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1369821.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="OQL90_003143"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="OQL90_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1369822.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..>10605)
                     /gene="eamB"
                     /locus_tag="OQL90_003144"
     CDS             complement(10047..>10605)
                     /gene="eamB"
                     /locus_tag="OQL90_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1369823.1"
                     /translation="TYTLITAMTPRPNNILALSSATSHGFRQSTRVLAGMSLGFLIVM
                     LLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQAKPISFWASFA
                     LQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCWALAGHLFQRL
                     FRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2638 a         2693 c         2727 g         2547 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgcc tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgtataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccatgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
    10141 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
    10201 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
    10261 acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg
    10321 attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc
    10381 cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact
    10441 gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc
    10501 cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga
    10561 atattgttcg gtcttggcgt catagcggta atcagggtgt aagtc
//
LOCUS       DAJVOY010000142        10454 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVOY010000142 DAJVOY010000000
VERSION     DAJVOY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.142"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(28..1926)
                     /gene="espL1"
                     /locus_tag="OQL90_003145"
     CDS             complement(28..1926)
                     /gene="espL1"
                     /locus_tag="OQL90_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCS1369824.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSELINQFARQLITLLD
                     TSDLSSMLSYCITQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQLVYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLIYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDETIDIFLNALTTPIAQELLNKKIVMDILAMKTRDGELGLFAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAHGTPVLYIAMSKGNEDVVLSYISTL
                     NIFAKKYSFSQRQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            2448..4376
                     /gene="thrS"
                     /locus_tag="OQL90_003146"
     CDS             2448..4376
                     /gene="thrS"
                     /locus_tag="OQL90_003146"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1369825.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4380..4922
                     /gene="infC"
                     /locus_tag="OQL90_003147"
     CDS             4380..4922
                     /gene="infC"
                     /locus_tag="OQL90_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1369826.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            5019..5216
                     /gene="rpmI"
                     /locus_tag="OQL90_003148"
     CDS             5019..5216
                     /gene="rpmI"
                     /locus_tag="OQL90_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1369827.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5269..5625
                     /gene="rplT"
                     /locus_tag="OQL90_003149"
     CDS             5269..5625
                     /gene="rplT"
                     /locus_tag="OQL90_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1369828.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5742..5866
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5748..5792
                     /gene="pheM"
                     /locus_tag="OQL90_003150"
     CDS             5748..5792
                     /gene="pheM"
                     /locus_tag="OQL90_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1369829.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6075..7058
                     /gene="pheS"
                     /locus_tag="OQL90_003151"
     CDS             6075..7058
                     /gene="pheS"
                     /locus_tag="OQL90_003151"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1369830.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7073..9460
                     /gene="pheT"
                     /locus_tag="OQL90_003152"
     CDS             7073..9460
                     /gene="pheT"
                     /locus_tag="OQL90_003152"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1369831.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9465..9764
                     /gene="ihfA"
                     /locus_tag="OQL90_003153"
     CDS             9465..9764
                     /gene="ihfA"
                     /locus_tag="OQL90_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1369832.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(10068..10208)
                     /locus_tag="OQL90_003154"
     CDS             complement(10068..10208)
                     /locus_tag="OQL90_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCS1369833.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2748 a         2365 c         2666 g         2675 t
ORIGIN      
        1 taagttggca gcattaccaa tagttggtta taaatacgtc tttagttcat cagcactaaa
       61 actcaggctt tggctgatta catttatagc agcataatat gtttctacag ttttataatg
      121 attatgatga atggctatat gaacagctga catgttgtca tgatttttag cggccaacag
      181 tgtgaataac tgacgttgac taaaagaata tttttttgca aaaatattca gcgtcgatat
      241 ataagataac acgacgtctt cattaccctt gctcatggcg atgtataata caggggttcc
      301 atgtgctgta gcgcctttta ataaatccat gatgttagct ttgctcaact tgtatttaaa
      361 ggcgatgcca ttaattttag agaggaaccg tgtgacacac aaagggtgat tattttccat
      421 tgcagcaaac aaccctagtt caccatcacg tgttttcatt gctaaaatat ccatcactat
      481 ttttttgttt aaaagctctt gtgctattgg agtggttaat gcattcagaa agatatcgat
      541 tgtttcatcg aacccataca acatggagat atagagccca ggtgtttcgt tagcactttt
      601 agtttgcagt aatttaacca gattatcttc atgaatgata tgattgtata tcagtgactg
      661 aatttcttgc ataaatattt ttacgttgtc agtatgtccg ttcatcatag ccagaaataa
      721 agcaggtgta ccaacgctat tctgcgcagt tattatttca atgatttctt tttcagggag
      781 tatgggcaac tgtttgaata atgtaataag ctgttgagtc tggcctgtgg ccattattaa
      841 caacattgcc gatggatgta taggggcaaa tggcattcgt gatgcaatac ccgcgaaaat
      901 agcccctgga actcccacag gtaatagggg cagaatagca attgcacagt tgcgaacata
      961 cctatcagcc caggtctgag catcgggacc tgaactgatg aacttatcaa gcgaaagaaa
     1021 gccccgtgct gtcctgtggg tgcctctgac ggcaacgttg gtatcgttcg ggtcgtagac
     1081 ggacactaca tagtttaatt gtccatacac taactgcttt attcgtagcc ttactgacaa
     1141 agtatgattg tctatatata aaatcgcgga catatgactg acattgtttg ccatcattcg
     1201 acaaaagaaa atatctaaag cgaagccaaa atccttactc gcgataacgc gactccctgc
     1261 aggaggatgg cgaagtaatg ctctgaactc cgcatcccgg actaagggga catgctgctg
     1321 tatggattca acggaggagt aatgtttaat caggttgact ttgccttttc catttctcaa
     1381 tgaatcaagg gcgtattgca atgacagatg gcgacaaaaa atagcgtcag aaggagattg
     1441 caaaaaacat gcactaccat tgagatttac tgttggttgt atagtttttt ctctatggat
     1501 ttttttagac agtttcatac aatggttacg atcttcggca gttaactgtt cctgctcaat
     1561 ataatccagt atgcttcgca aatggttagc cttaataggc gtggtttcca gaaataaggg
     1621 aatgatttct tttttatagt gtaatgtaat aagtatattt tctgcatgga tgggtgaagt
     1681 ggcaaagtta atgataaaaa gggcagtgga gtaacaattt tgtgatattt ttcgacagtt
     1741 tgcggtaaat tcctgagtaa tacagtatga cagcatcgaa cttaagtcag aggtatcaag
     1801 tagggtgatg agttggcgcg caaattgatt aataagctct gatgaaggcg ttggtatgtc
     1861 gctatcgatg tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg
     1921 actcataatc tattcacctt atatatactg gaggttttga ggatgatgat taattattat
     1981 cgccagaacg aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg
     2041 atatgaccac ttcgtcatta atctcatgat cgtttaaata ctaccgccaa atcacgcaac
     2101 gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac ctttcagacg
     2161 caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact acaaacgaat
     2221 tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag tgttaacatc
     2281 gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg attgcgaacc
     2341 aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca aatttttctt
     2401 tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg cctgttataa
     2461 ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg gatgttgcgc
     2521 tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat ggcgaactgg
     2581 ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc gccaaagacg
     2641 aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat gcgattaaac
     2701 aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt ttttattacg
     2761 acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag aagcggatgc
     2821 atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg cacgcagcgc
     2881 gtgaaacttt cgccaaccgt ggggagagct acaaagtctc cattcttgac gaaaacatcg
     2941 cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg tgccgcggtc
     3001 cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg gcaggggctt
     3061 actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg gcgtgggcag
     3121 acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa cgcgaccacc
     3181 gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg ggtatggtat
     3241 tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt cgttctaaac
     3301 tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt gtcctgtggg
     3361 aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct tctgagaacc
     3421 gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc aaccaggggc
     3481 tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc caccgtaacg
     3541 agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccaa gatgacgcgc
     3601 atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc cgtttagtct
     3661 atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc actcgtcctg
     3721 aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg gcggttgcgc
     3781 tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc tacggtccga
     3841 aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt acagtacagc
     3901 tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac aacgagcgta
     3961 aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggaacgtttc atcggtatcc
     4021 tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag gttgttatca
     4081 tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa ctatcaaatg
     4141 cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa atccgcgagc
     4201 acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg gaatcaggca
     4261 aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta aatgaagtga
     4321 tcgagaagtt gcaacaagag attcgcagcc gcagtcttaa acaattggag gaataaggta
     4381 ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc gaaattcgcg
     4441 cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg agtctgagag
     4501 aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc cctaacgccg
     4561 agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag agcaagtctt
     4621 ctaaggaaca gaagaaaaag caaagagtta tccaggttaa ggaaattaaa ttccgtcctg
     4681 gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt ctcgaagagg
     4741 gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg
     4801 gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg gtcgaatcct
     4861 tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag aagaaacagt
     4921 aaggccttca agtagcactg tctgtggagc cttcgggctt cacaggcttt gttcgccttt
     4981 gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt aagaccgtac
     5041 gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac aagcacgcta
     5101 acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg cgtccgaaag
     5161 ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac gcataagccg
     5221 ttaacgtttt taacttttta attagaatat agatacagga gagcacatat ggctcgcgta
     5281 aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc taaaggctac
     5341 tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa agctggtcag
     5401 tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg gattgcacgt
     5461 atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa tggcctgaaa
     5521 aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt cgacaaagta
     5581 gcgttcaccg ctctggttga aaaagcgaaa gcagctctgg cataagccag ttgaaagagg
     5641 gagcttgtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta tcgccgtagc
     5701 cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg aatgctgcta
     5761 ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg tgagaagaga
     5821 aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc gcgttttgaa
     5881 aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggatac ggccagcgcg
     5941 gagaaaccgg agcgtattta agtacgtgag aatttcgagc actgcccggg accaaaatgg
     6001 caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc accactgaca
     6061 caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag gcggccatta
     6121 gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg ggtaaaaaag
     6181 ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag cgtccggcag
     6241 ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat gcgcgtaaag
     6301 cggaactgga aagcgcagcg ctgaatgcgc gtctggcggc ggaaacgatt gatgtctccc
     6361 tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc atcgaccgta
     6421 tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa atcgaagacg
     6481 attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc gctgaccacg
     6541 acactttctg gtttgacact acccgcctgc tgcgtaccca gacctctggc gtacagatcc
     6601 gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgc gtttatcgta
     6661 acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg attgttgata
     6721 ccaacatcag ctttaccaac ctgaaaggca ctctgcacga tttcctgcgt aacttctttg
     6781 aggaagattt gcagattcgc ttccgtcctt cctacttccc gttcaccgag ccttctgcag
     6841 aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggttgc gggatggtgc
     6901 atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt ttcgccttcg
     6961 ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg cgttcattct
     7021 tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga ttatgaaatt
     7081 cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgatg cgctggcgaa
     7141 tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg gtagcttcca
     7201 cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg acaaactgcg
     7261 tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgtg gtgcgccaaa
     7321 ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc cgggtgattt
     7381 caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt gctccttctc
     7441 tgaactggga atttctgacg atcacagcgg cattatcgaa ctgcctgcag atgcgccgat
     7501 tggcactgac atccgcgaat acctgaaact cgatgacaac accatcgaaa tcagcgtaac
     7561 gccaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg ccgtgctgaa
     7621 tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg acgacacgtt
     7681 gccgattaca gtcgaagcgc ctgaagcctg cccgcgctat cttggccgtg tggtaaaagg
     7741 cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc gttgcgggat
     7801 ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac tgggccagcc
     7861 gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga tggcaaaaga
     7921 gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg acactctggt
     7981 catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg aacactctgg
     8041 cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc cgctgtctat
     8101 caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt atgagcgtgg
     8161 cgttgatccg gcactgcagc acaaagcgat ggaacgtgcg acccgtctgc tgatcgacat
     8221 ctgcggtggt gaggctggtc cggtaattga tatcaccaac gaagcaacgc tgccgaagcg
     8281 tgcaaccatc actttacgtc gtagcaaact ggattgcctg atcggccatc atattgctga
     8341 tgaacaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag gcaaagacga
     8401 gtggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag atctggtcga
     8461 agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac aggcaagcct
     8521 gattatgggt actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa cgctgctcaa
     8581 cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag tgcagcagat
     8641 gatccatcca ggcgttgaag ccttactgct gccaagcccg atctctgttg aaatgtcagc
     8701 aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc agaaccgtca
     8761 gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata ctcaggcacc
     8821 gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc gttacgaaga
     8881 gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg atcttgaatc
     8941 cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag cgaatccggc
     9001 actgcatccg gggcaatccg cagcgattta tctgaaaggg gaacgtattg gttttgttgg
     9061 agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc tggtgttcga
     9121 actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga tttctcgctt
     9181 cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg cagcggatat
     9241 tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact tatttgacgt
     9301 gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc tgatcctgca
     9361 agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca aatgtgtaga
     9421 ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg cttacaaaag
     9481 ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat gccaaagaac
     9541 tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa caggtgaaac
     9601 tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga cgtaacccga
     9661 aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga cccgggcaga
     9721 agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatcagat ctaactaaaa
     9781 aggccgctct gcggcctttt ttctttcaat aactaattga tttataatgt tttatatctc
     9841 ttcgttccac gatgcgtcca catttagtgg ggggagggga gtaagcccgc tacattgtgg
     9901 gctttgtctt atagtctgat cactgtttca agtcggtgag gacaccaaaa agaacccccg
     9961 cagtttttac gctgcgggtt tgttgttcat gtctgtgaga tagggtgcct tatcgacctt
    10021 accctggcaa ccgattgacg ggggattgct cccccgtcgc ggtttcctta ctgcttacac
    10081 tgtaagaacg ccgcaaactc cgctccccag aagctcatcc gtatttcgca cagcgaaccg
    10141 tgcagcatcc agatgatgag gattgccgtc acgcagaacg tgatggccgt aagcgatttt
    10201 tgcgacatag cgcttgctcc tttttcgggg aggcgctaac ctatcacttg cttagggtag
    10261 acggttaggg cctcggttaa acaaaaatgt tttccggggc ctttccacat ctggccttca
    10321 ggtattccct ccgaccatca gccgaaaggc acccgcgcgt aatctatcgc ttttttgtta
    10381 ctccggcaat tctgctcgtt aattctgagg taagggcaaa ctcatctgat tgtttcctct
    10441 gtgtgaagtt ggca
//
LOCUS       DAJVOY010000143        10313 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVOY010000143 DAJVOY010000000
VERSION     DAJVOY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.143"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            112..978
                     /gene="folD"
                     /locus_tag="OQL90_003155"
     CDS             112..978
                     /gene="folD"
                     /locus_tag="OQL90_003155"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1369834.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQACIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            980..1192
                     /gene="ybcJ"
                     /locus_tag="OQL90_003156"
     CDS             980..1192
                     /gene="ybcJ"
                     /locus_tag="OQL90_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1369835.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1300..1821
                     /locus_tag="OQL90_003157"
     CDS             1300..1821
                     /locus_tag="OQL90_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1369836.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMEMLWWRRR"
     gene            complement(1857..3242)
                     /gene="cysS"
                     /locus_tag="OQL90_003158"
     CDS             complement(1857..3242)
                     /gene="cysS"
                     /locus_tag="OQL90_003158"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1369837.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3416..3910
                     /gene="ppiB"
                     /locus_tag="OQL90_003159"
     CDS             3416..3910
                     /gene="ppiB"
                     /locus_tag="OQL90_003159"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1369838.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3913..4635
                     /gene="lpxH"
                     /locus_tag="OQL90_003160"
     CDS             3913..4635
                     /gene="lpxH"
                     /locus_tag="OQL90_003160"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1369839.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4753..5262
                     /gene="purE"
                     /locus_tag="OQL90_003161"
     CDS             4753..5262
                     /gene="purE"
                     /locus_tag="OQL90_003161"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1369840.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5259..6326
                     /gene="purK"
                     /locus_tag="OQL90_003162"
     CDS             5259..6326
                     /gene="purK"
                     /locus_tag="OQL90_003162"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1369841.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6463..7354)
                     /locus_tag="OQL90_003163"
                     /pseudo
     CDS             complement(6463..7354)
                     /locus_tag="OQL90_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7351..8166)
                     /locus_tag="OQL90_003164"
     CDS             complement(7351..8166)
                     /locus_tag="OQL90_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS1369842.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8177..9436)
                     /locus_tag="OQL90_003165"
     CDS             complement(8177..9436)
                     /locus_tag="OQL90_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS1369843.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9446..10313)
                     /gene="fdrA"
                     /locus_tag="OQL90_003166"
                     /pseudo
     CDS             complement(9446..10313)
                     /gene="fdrA"
                     /locus_tag="OQL90_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2270 a         2927 c         2770 g         2346 t
ORIGIN      
        1 aagaggtcgc ttcacgcgat aaatctgaaa cgaaacctga cagcgcgccc cgcttctgac
       61 aaaataggcg catccccttc gacctacgta acagatggaa tcctctctct gatggcagca
      121 aagattattg acggtaaaac gattgcgcag caggtgcgct ctgaagttgc tcaaaaagtt
      181 caggcgtgta ttgcagccgg actgcgggca ccaggactgg ccgttgtgct ggtgggtagt
      241 aaccctgcat cgcaaattta tgtcgcaagc aaacgcaagg cttgtgaaga agtcgggttc
      301 gtctcccgct cttatgacct cccggaaacc accagcgaag cggagctgtt ggagcttatc
      361 gatgcgctga atgccgacaa caccatcgat ggcattctgg ttcaactgcc gttaccggcg
      421 ggtattgata acgtcaaagt gctggaacgt attcatccgg acaaagacgt ggacggtttc
      481 catccttata acgtcggtcg tctgtgccag cgcgcgccgc gtctgcgtcc ctgcaccccg
      541 cgcggtatcg tcacgctgct tgagcgttac aacattgata ccttcggcct taacgccgtg
      601 gtgattggcg catcgaatat cgtcggtcgt ccaatgagca tggaactgtt gctcgcaggt
      661 tgcaccacta ccgtgactca ccgtttcact aaaaatctgc gtcaccacgt agaaaatgcc
      721 gatctgttga tcgttgccgt cggcaagccg ggctttattc ctggtgactg gatcaaagaa
      781 ggcgcaattg tgattgatgt cggcatcaac cgtctggaaa atggcaaagt tgtgggcgac
      841 gtcgtgtttg aagacgcggc taaacgcgcc tcatacatta cgcctgttcc gggcggcgtt
      901 ggcccgatga cggttgccac gctgattgaa aacacgcttc aggcgtgcgt tgaatatcat
      961 gatccacagg gtgagtaaca tggcgacatt ttctttaggt aaacatccgc acgttgagct
     1021 gtgcgatttg ctgaaactgg aaggctggag cgaaagcggc gcgcaggcga aaatcgcgat
     1081 tgccgaaggc caggtgaaag tcgacggtgc ggttgaaacg cgcaaacgct gcaaaatcgt
     1141 cgccggtcag acagtgagtt ttgcaggtca cagcgtacag gttgttgcct gatccctttc
     1201 ccggcctgta tcaacaggcc ggttctcttt tcagactttt gccctctacg ccccgttcat
     1261 ccatgctgat agcatcgaca ttttgccgaa gcgaccatca tgccaaccgt tattacgcac
     1321 gccgccgttc ccctatgcat tggtttaggg ttgggttcga aagtcatccc tccgcgtctg
     1381 ttatttgccg gaatcatcct ggcgatgctg cccgacgccg acgtattgtc gtttaaattt
     1441 ggcgttgctt acggcaatgt ttttggtcat cgcgggttta cccattcgct ggtgtttgcg
     1501 tttgttgtcc cgctgttatg tgtgctgatt ggacgccgat ggttcagggc tgggcttatt
     1561 cgctgctggc tgtttttaac cgtctcgttg ctatcgcaca gcttgctgga ttcggtaacc
     1621 actggcggta agggcgttgg ctggctgtgg ccgtggtcag atgaacgctt tttcgctccc
     1681 tggcaggtga ttaaagtcgc gccgtttgca ttgtctcgtt acaccacgcc gtacgggcat
     1741 caggtgataa tttctgaatt gatgtgggtg tggttgccgg ggatggtgct tatggaaatg
     1801 ttgtggtggc gcaggcgata accggatgcg aaaactcgca tccggcaaca gcgcaattac
     1861 ttacgacgcc aggtggtccc ttgcgggcca tcttccagca cgatccccat ctcgttaaga
     1921 cggtcacgcg ccgcatctgc cgccgcccag tctttcgctt tacgggcatc cagacgctgt
     1981 tgaattaacg cttcaatctc agccacttcg ctgtcgtctg cctgcgcgcc gctttgcagg
     2041 aacgcttccg gttcttgctc cagcaggccc aatacagcgg aaagtttacg caggtgagat
     2101 gccattgcat tcgccgctgc catatcttct gctttcagac ggtttacttc acgcgccata
     2161 tcaaacagta cggaataggc ttccggggtg ttgaaatcgt cgtccatcgc ttcaataaag
     2221 cgcgcttcaa acgcttcgcc accggcaggc gcaacggttt tatctgtgcc gcgcagcgca
     2281 gtgtagagac gctccagcgc cgcacgcgtc tgcttcaggt tctcttcgct gtagttcaac
     2341 tggctgcgat agtggcccga catcaggaag taacgcacgg tttccgcgtc gtagtatttc
     2401 agcacatcgc gcacggtaaa gaagttaccc agcgatttgg acatcttctc gcggtcaacc
     2461 atcaccattc cggagtgcat ccagtagttc acatactgac catcatgggc acaggtggac
     2521 tgcgcgattt cgttctcgtg gtgcgggaac atcaggtctg aaccgccgcc gtggatatca
     2581 aagtggttac ccagctgctt gcagttcatg gccgaacatt caatgtgcca gccaggacga
     2641 cccgcgcccc acggagacgg ccagctcggt tcgccctctt tcgacatctt ccacagaacg
     2701 aagtccatcg ggttgcgttt gtcatcaacc acgtcaacgc gcgcgccagc ctgcagctgg
     2761 tcgagatcct gacgcgacag cacgccataa gtcggatcgg tcgggacgtc gaacatcacg
     2821 tcgccgttgt cagccacata agcgtgacct ttggcgatca gttgttcagt gagttcaata
     2881 atttctgcga tatggtgcgt cgcgcgcggc tccatatccg ggcgcagaat gttcaaagca
     2941 tcaaaatctt tgtgcatttc ggcgatcatg cgatccacca gcgccacaaa gctttcgcca
     3001 ttttcattgg cgcgtttgat gattttgtcg tcgatatcgg taatgttgcg cacatacttc
     3061 agcttatagc cgaggaaacg cagatagcgc gcaaccacgt caaaagcaac aaaggtacgc
     3121 ccgtgaccga tatgacagag atcgtaaacg gtgattccac acacgtacat gccgacttcc
     3181 ccggcgtgaa taggcttaaa ttcctctttt tggcgtgtca gagtattgaa gatttttagc
     3241 atcgaagatt ccgtttagac atgtgtgggt aattgagttg cgtataatac ccatatttcc
     3301 cgcgcgaatc agcatacatt gcgtgatgat ccgatcgtgc ggttatgcta taacaccacc
     3361 ctatatatga cccgaaccgg gttgaagcac caatcaaacg gaacaggatg caaaaatggt
     3421 tactttccac accaatcacg gcgatattgt catcaaaact tttgatgata aagcacctga
     3481 aacagttaaa aacttcctgg actactgccg cgaaggtttt tacaacaaca ccattttcca
     3541 ccgtgttatc aacggcttta tgattcaggg cggcggtttt gaaccgggca tgaaacaaaa
     3601 agccaccaaa gaaccgatca aaaacgaagc caacaacggt ctgaaaaata cccgtggtac
     3661 gctggcaatg gcccgtactc aggctccgca ctctgcaact gcacagttct tcatcaacgt
     3721 ggttgataac gacttcctga acttctctgg cgaaagcctg caaggttggg gctactgcgt
     3781 gtttgctgaa gtggttgaag gcatggacgt ggtagacaaa atcaaaggtg ttgcaaccgg
     3841 tcgtagcggt atgcaccagg acgtgccaaa agaagacgtt atcattgaaa gcgtgaccgt
     3901 tagcgagtaa tcgtggcgac actctttatt gcagatcttc atctctgcgt ggaagaaccg
     3961 gcgatcaccg ccggttttct gcgtttttta gcgggggaag ctcgcaaggc cgacgcgctg
     4021 tatattcttg gcgatctgtt tgaagcgtgg attggcgacg acgatcccaa tccactccat
     4081 cgccagatgg cggcagcgat caaagcggtg tccgattccg gcgttccctg ttatttcatt
     4141 catggcaacc gtgattttct gctcggcaaa cgctttgccc gtgaaagtgg catgacgtta
     4201 ttgccggaag aaaaggtgct cgaactttat ggccgccggg tgttgattat gcatggcgac
     4261 acgctgtgca ccgatgacgc gggttatcag gcttttcgcg ccaaagtcca caaaccctgg
     4321 ctgcaaatgc tattcctcgc cctgccgttg tttgtgcgca aacgcattgc cgcgcgaatg
     4381 cgcgcgaaca gcaaagaagc caacagcagt aaatcgctgg cgatcatgga cgttaaccaa
     4441 aacgcagtgg tcagtgcgat ggaaaaacat caggtgcaat ggctgatcca cgggcatacc
     4501 catcgcccgg cggtgcatga acttatcgcc aaccagcaac ctgcttttcg cgtggtactg
     4561 ggtgcctggc atacggaagg ttcgatggtg aaagtcacgg cggatgacgt tgagctgatt
     4621 cattttccgt tttaaaaaaa ccacaacttt gctgatttta cagccacgca accgttttcc
     4681 ttgctctctt tccgtgctat tctctgtgcc ctctaaagcc gagagttgtg caccacagga
     4741 gttttaagac gcatgtcttc ccgcaataat ccggcgcgtg tcgccatcgt gatggggtcc
     4801 aaaagcgact gggctaccat gcagttcgcc gccgaaatct tcgaaatcct gaatgtcccg
     4861 caccacgttg aagtggtttc tgctcaccgc acccccgata aactgttcag cttcgccgaa
     4921 agcgccgaag agaacggtta tcaggtgatt attgcgggcg caggtggcgc agcgcacctg
     4981 ccaggcatga ttgccgccaa aacgctggtg ccggtgctgg gcgtgccagt acagagcgcc
     5041 gcactgagcg gtgtcgatag cctctactcc atcgtacaaa tgccgcgcgg cattccggtg
     5101 ggtacgctgg cgattggtaa agctggcgcg gcaaacgcgg cattactggc agcacaaatt
     5161 ctcgcgactc acgataaaga gctacaccag cgtctgaatg actggcgcaa agcccagacc
     5221 gacgaagtgc tggaaaatcc ggacccgcga ggtgcggcat gaaacaggtt tgcgtcctcg
     5281 gtaacgggca gttaggccgt atgctgcgtc aggcaggcga accgttaggc attgctgtct
     5341 ggccggtcgg gctggacgct gaaccggcgg cggtgccttt tcaacaaagc gtgattaccg
     5401 ctgagatcga acgctggccg gaaaccgtat taacccgcga gctggcgcgt catccggcct
     5461 ttgtgaaccg cgatgtgttc ccgattattg ctgaccgtct gactcagaag cagcttttcg
     5521 ataagctcca cctgccgacc gcgccgtggc agttacttgc cgatcgcagc gagtggcctg
     5581 cggtgtttga tcgtttaggt gaactggcga ttgttaagcg tcgcactggt ggctatgacg
     5641 gtcgcggtca atggcgttta cgcgccaatg aaaccgaaca gttaccggca gagtgttacg
     5701 gcgaatgtat tgtcgagcag ggcattaact tctctggtga agtgtcgctg gttggcgcgc
     5761 gcggctttga tggcagcacc gtgttttatc cgctgacgca taacctgcat caggacggta
     5821 ttttgcgcac cagcgtcgct tttccgcagg ccaacgcaca gcagcaggcg caagccgaag
     5881 agatgctgtc ggcgattatg caggagctgg gctatatggg cgtgatggcg atggagtgtt
     5941 ttgtcacccc gcaaggtctg ctgatcaacg aactggctcc gcgtgtgcat aacagcggtc
     6001 actggacaca aaacggtgcc agcatcagcc agtttgagct gcatctgcgg gcgattaccg
     6061 atctgccgtt accgcaacca gtagtgaata atccgtcggt gatgatcaat ctgattggta
     6121 gcgatgcgaa ttatgactgg ctgaaattgc cgctggtgca tctgcactgg tacgacaaag
     6181 aagtccgtcc ggggcgtaaa gtggggcatc tgaatttgac cgacagcgac acatcgcgtc
     6241 tgactgcgac gctggaagcc ttaatcccgc tgctgccgcc agagtatgcc agcggcgtga
     6301 tttgggcgca gagtaagttc agttaagcag gtgctcattt gattgccgga tgcggcgtaa
     6361 acgccttatc cggcttaccg ctccggtacc cattgtaggc ctgataagac gcgtcaagcg
     6421 tcgaatcagg catggtgcac cgattgccgg atgctctcat cactacagcg aaatacaggt
     6481 ccccgcttcg cccgccagcg tctcttcaat tcgcgataac gccccaatcc acgcgggttt
     6541 actacggctt ctgacataac cactcaccgc cgttaccttc ggccccatcg aaccatcggc
     6601 tttggcaaat ggcgctaact catccggtgt ggcatggcga atggcacgtt gctgcggcgt
     6661 tccccagttt tcatataccg catcagcatc ggtgaggatc accagtccat cggcattaat
     6721 ctgctcggcg agcaacgcag tggcgagatc tttatcaatc accgcttcac tccctgcccc
     6781 atcttccgtc acaggcacac cgccgccgcc actgcaaatc accacatgcc cctctttgag
     6841 caacaactcg atggcttcgc tgtcgagaat tttacgcggt tgcggagacg ccaccacccg
     6901 gcgcaaatat ttaccatcac gtttcatctg ccagccgtaa gccgcttcca gtgcctcttg
     6961 ttcttctggc tgataaaccg gaccaataaa tttctctggc tgcaaaaacg ccggatcatc
     7021 aggcgaaacc tcaatgcgcg tcagcaccgt cgtaacaggc ggtatctgcg gctgtgcgct
     7081 caagctctgc gccagcatat agcctcatcc cctggctttc cgccaccagc acatccagcg
     7141 gatacggttc tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc
     7201 cgttgccgtg aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag
     7261 cactggcgat attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg
     7321 cgttgccccc aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc
     7381 aagccagaac cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc
     7441 aatcgcaaca gcggtacgtt tcgggcaact cagagcatgg acaaagtgca ggagtggcga
     7501 ggcgaaatat ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag
     7561 ttgaagattg tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta aagcgccata
     7621 ataccaagcc gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg
     7681 gcctttaccc agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca
     7741 gtgacgaaac tggcgtaatt cagcggtcag cggatcgctt gccgccatcg ccagtggtcc
     7801 gaaaagtccg gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcga
     7861 aggcggcgta atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag
     7921 gcgaatccct tgagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc
     7981 atcgaattgc gcgcggcgaa gcacccatcc tccggggccg aaaccactac cctgacgatg
     8041 cagcgttaac agttcgccgc tttccgtcat caggttaatc gcccgccgcc acactcccgc
     8101 taaccgccag gactggcgat aattcggtgc gctactgctg gcaagcagag gatggatgat
     8161 cgtcattgcg cgttcctcaa ccaatcccca tgctttcagc cagtgcttcc agcgcctgtt
     8221 caaagcacgc cagcggtgcc cgcacggtgc ctgcgccaat ctgcccgatc cccgcctctt
     8281 tatgggcgat accggtattg atgagtggcg taatgccggt ttctaccacg cgacgaatat
     8341 ccagccccag gcacgcgccc tgaaaatccc agcctgggat ctgcaactgc atattgcgtt
     8401 caaggtaaat ttccgccatc tcttcagata ccgccctggc cgcttccatg ccacccgcac
     8461 cgacaaagcg cgttacgcca ggcgctgcga tcattgccgc acctccgata ccaaaggttt
     8521 cggtaatcgc gctatcgccc atatccgggt tcgcctgctc ctgcgagaag ccggtgaaaa
     8581 acagaccttg cggagtgttt acaggcgcag taaaccagcg ttcacccagc ccgcttaccc
     8641 gaatcccgaa catattgccg ttgcgggtca tagccgtaac gatgctgcct gcgcggatca
     8701 tcgcgccagc atccatcgcc gccttgcagt aggccatcgc gaggttgagg aagaactgat
     8761 cggtcacgct gaggaaatcg atcacttccg caatgtgctg tttatcatga tcgaggcgag
     8821 caatttgtgg ggccagcgta cgcattaaca gtgctgagga agcaatattg cgttgatgga
     8881 actcatcgcc catcgtaatg ccctgcgcca tcatcgccgt gagatcgata ccgcgctcca
     8941 tgcgccccag cgccgcgctt aatactggca ttaacacatc gcgcatccag cgatggcgag
     9001 tcaggacatc ttcgccgtaa gcgccaaaac gcatcacttt gccgatacct tcgttgaggt
     9061 tgcagtacgc ccggttgccg tcggtcacgt tctcgaccac cagcatcggc atactggcag
     9121 aagtaatgcc gcccattggc ccgacggcat tcacatggtg acaaggaatg aagttcactt
     9181 ccccctgctc cagcatcgcc agtgcctgcg cttcatcttt cgcccagcct tcgaacagac
     9241 atccgcccac gcacgcccct ttcatgggtc cggtcatctc ctgccagcgc attggcggcc
     9301 cggcgtgaag cagtgttttg ccctcgttta gttcgctgat aagtgaaaaa gccggttgca
     9361 catccagcca gtgtggacga gcacgacgaa tttgttcgat aaccgcagca ttggcttgcg
     9421 ccactgatgt aaacatggga accccttatt gcaaacgttc taataaacga gccagttttt
     9481 tattgccgcc ggcgactggc gaccattggt aatgcacaac cggtttgctg gcgctttgta
     9541 gctccagcgc aaagctgcgt aatccgatgt taatcacggc gacgttttcc agtaatgacg
     9601 gtgtatgttg ctgtgtggca ggcgagagcg gataaattaa cgctgccgcc agcaaggtgg
     9661 cttccggcag cgaactcact accgcgatcc ccgcatcttc cagcgtggcg atttgctgcg
     9721 agcggcattg cgggtcacgt tcagtgcctg tcaccgtggc aatggcatac agtggttgat
     9781 tatctgaacg cgcggcacag gctttttgct aggcgcttac cagcgaggcg gcaggatcgg
     9841 cagtcgcacc gaagccaatc acgacatcaa gtagcaatac gcgcacttgt ggtttagcgc
     9901 cgagatcggc aattaactgg ttgcgtaagg ctgggtcgat catcggatgg ggacgcccga
     9961 cggtgtagaa atcatcgccg aggtcaataa tctggtgacc atcggcgtcg agcatcatgc
    10021 cgtgatgatg ggtatcgtcg gcttccacgc caaggtgtcc agcaagtaat cccgccgctt
    10081 cggcagccag cgtaccgccg gtatacaaac cgcaaataaa tccgctgctg acaggcgcta
    10141 ttgcgttaca tcgcgccgtg acgcgtgaaa gcaggcaagc caggcgtgcg gcctcatcca
    10201 gcgaggaggc aaaccagaca ttctcgtcgc gggccaccgc cggggtataa cctaaaaaca
    10261 gcgccaccat cggtttgccg gttgctttca tggcattaac aattttcaga cgc
//
LOCUS       DAJVOY010000144        10301 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVOY010000144 DAJVOY010000000
VERSION     DAJVOY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.144"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1258
                     /locus_tag="OQL90_003167"
     CDS             <1..1258
                     /locus_tag="OQL90_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCS1369844.1"
                     /translation="GTHVNYAGAFASAFPNGLPVGIGSGLLFTGKQGDALTFATITDR
                     GPNADSPKEGKNETKIFVTPDFAPLLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLAS
                     DVIGSTNEVAFSDTLHRLKGDNRGLDTEGITPDGKGGYWLCDEYGPFLINIDSKGKIL
                     AIHGPQAAEGEKAIAGGLPNILKWRQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKK
                     KALFTRLVSFDPASGKTAMYGYPIDSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKN
                     NRMRNLIYEVDLNKASDLSGFDKPGEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQ
                     QEKAEGLALIDSKTLAVANDNDFGVKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVET
                     TLRVKPLEKPESDSELWIVTLPEALK"
     gene            complement(1497..2882)
                     /locus_tag="OQL90_003168"
     CDS             complement(1497..2882)
                     /locus_tag="OQL90_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1369845.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(2932..3279)
                     /gene="tnpB"
                     /locus_tag="OQL90_003169"
     CDS             complement(2932..3279)
                     /gene="tnpB"
                     /locus_tag="OQL90_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1369846.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3276..3665)
                     /locus_tag="OQL90_003170"
     CDS             complement(3276..3665)
                     /locus_tag="OQL90_003170"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCS1369847.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4011..4445)
                     /locus_tag="OQL90_003171"
     CDS             complement(4011..4445)
                     /locus_tag="OQL90_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCS1369848.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4433..4834)
                     /locus_tag="OQL90_003172"
     CDS             complement(4433..4834)
                     /locus_tag="OQL90_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369849.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5094..5663)
                     /locus_tag="OQL90_003173"
     CDS             complement(5094..5663)
                     /locus_tag="OQL90_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCS1369850.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6408..6551)
                     /locus_tag="OQL90_003174"
                     /pseudo
     CDS             complement(6408..6551)
                     /locus_tag="OQL90_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(6885..7151)
                     /locus_tag="OQL90_003175"
     CDS             complement(6885..7151)
                     /locus_tag="OQL90_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369851.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7292..7498
                     /locus_tag="OQL90_003176"
     CDS             7292..7498
                     /locus_tag="OQL90_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1369852.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            7593..8195
                     /locus_tag="OQL90_003177"
     CDS             7593..8195
                     /locus_tag="OQL90_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369853.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            9395..>10301
                     /locus_tag="OQL90_003178"
     CDS             9395..>10301
                     /locus_tag="OQL90_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369854.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYR"
BASE COUNT         2478 a         2722 c         2621 g         2480 t
ORIGIN      
        1 aggcacgcac gtgaactacg ccggcgcatt tgccagtgca ttcccgaacg gccttccggt
       61 tgggataggt tctggcctgc tgttcaccgg taaacagggc gatgccctga cattcgcaac
      121 cattaccgat cgcggtccga acgcggattc accgaaagag gggaaaaacg aaactaaaat
      181 tttcgttacg ccagatttcg cgccgctgct gatgacgatt cgtgtacaaa acggcaaagc
      241 ggaggccatc gatccccgtc ctctgcatga cgataaaggc gcgatcaacg gtctgccgct
      301 ggcaagcgac gtgattggct ccaccaatga agtggccttc agcgacacgt tacatagact
      361 gaagggggat aatcgcgggc tggatacgga agggatcact ccggatggta aaggaggtta
      421 ctggctgtgc gatgagtatg gcccgttcct gattaacatc gacagtaaag ggaaaatcct
      481 ggcaatccac ggtccgcagg cggcggaagg ggaaaaagcg attgcgggcg gtttgccaaa
      541 tatcctcaaa tggcgtcagg cgaaccgcgg ctttgaaggc ctcacccgta tgccggacgg
      601 gcgtattatt gtcgccgtgc aaagtacgct ggatatcgac gctaagagca agaaaaaggc
      661 gctgtttacg cgtctggtga gcttcgatcc ggcaagcgga aaaaccgcga tgtacggtta
      721 tcctatcgac agcgcggcct acagcaaaaa cagcgacgct aaaatcggag acatcgtggc
      781 gctcgataac cagcacatcc tgctgatcga acagggaaga gataaaaaca acagaatgcg
      841 taacctgatt tacgaggtcg atctcaataa ggcgagcgac ctgtcaggct tcgacaagcc
      901 gggtgagtat ccggagtttg atgatgagaa aaccctgtca cagcgcggca ttacccttgc
      961 gcagaaaacg caggttgttg atctgcgctc cttgggctgg cagcaggaga aggctgaagg
     1021 gttggcgctg atcgacagca aaacgctggc ggtggcgaac gataacgact ttggcgtgaa
     1081 ggtggcgatg caacatccgg tcgagggcaa gacattcaag gattatcggg tgaacgcgga
     1141 aggaaagctg acgcttgacg acaagcaggt tgaaacgacc ctcagagtga agccactgga
     1201 gaaaccggag tctgacagtg aactgtggat tgtgacgctg ccggaagcgt tgaaataagt
     1261 ttataaggta cggacagcag tatcgcttag tttttcaaat tcatgtatgt gaacaatatt
     1321 ggggttccgg cgtagacccc caatgaaagc gtcaccacgt gaaaacatgc ctgcacggtt
     1381 tacacgtcat cccatggtgg cagccggtta actttcctgc gccaccagtg gaaaaaggcc
     1441 ataagcagtc cgaacaaaag taagcgtaca gcgagggccg tattgacggg gatgtgttat
     1501 tcagctggca gtgctatgcg ccacggaagc agttcgctga cccggttgac cggccagtct
     1561 gctatgacgc caagcacatg gcgaaggtag ctttctggat ccacgtcatt cagtttgcac
     1621 gtcccgatca ggctgtacag tagcgctccc cgctcaccac catggccaga gccgaagaac
     1681 agggtcttca tcttttcacg caaccagctt tccagggatt tcaacagcgg tttcgttttt
     1741 cgctgacgtt cagcaagccg ctgctctgcc ggcattcccc ttatatccgc ctctatggcg
     1801 tacaactgac cgatctgctc cagggcttct tccgtcagtg ctgacgggat gcggacgtgc
     1861 acatcgtgga tctttcggcg ggcatgagcc cagcaggcag cttccgttat cccaccattg
     1921 cgatacagct cgttgaaccc ggcgtacgca tccgcttgca gcacaccgct gaagcaagca
     1981 agatgagtct gcggatggat gctttttctg tccgggctgt aagcgaacca cactgcaggt
     2041 gccaacgctg acccggcatt gcggtcatca cgaacatacg cccacaaccg cccggtcttc
     2101 gtcttcttat tacccggcag cagtacctgg accggggtat catcggcatg gagtttgccg
     2161 tcagtcatga catagccatg aagcgcctct tccagcggag acagcagccg gcagcatgca
     2221 tccacccagc ccgacagcag tgaacggctc agctccacac cttgccggcc gtatatttct
     2281 gactggcaat acagcggggt gtgctctgca tacttcgagg tcagcacgcg ggccagcagc
     2341 cccggtccgg cgataccccg ctcgatgggc cgcgaaggtg caggtgcctg cacgatggta
     2401 tcgcactgag tacaggcatg tttttcccgt accgtccgga taacccggaa ggcgctacgc
     2461 atcaactcca gctgttcggc ggtatcctcg cccagatagc tcagtgaacc gccgcagttc
     2521 gggcagcacg gcgccgcagg caacagtcgc ttttcgtcac ggggtagtga ttcagggaac
     2581 ggcttacggg tgcgggtctg acgcaacgga cgctgtactg ccgggtcata caccctacca
     2641 gtcagcgtat cgctctcttt ctgaagccgg ttcagatcgg cttccatttg tgcgatacgg
     2701 cgggagactt tttcggaacg actgccgaag ttcatccggc ggagtttatc cagctgcgcc
     2761 tgcagatggt ctatttcgcg ctcccggttg ctcagctttt cctgcagggc gtggatcagc
     2821 gcttcctgtt cggccaggcg ctgtttcagc aggaagatgt cgtcagaaga gatgtcgttc
     2881 ataagcccgt attttaccgg gcttattctg tgacaaccag gataaagaga tttacagcat
     2941 ggtcagggag gtcagcagcc gcttgggctg tcgccagtcg ataccttcca gcagcatcgc
     3001 cagctgcgcc tgcgtaagga acactttgcc atcacgggct gacggccagg cgaagcgccc
     3061 acgctccagc cgtttggtca ggaggcacag tccgtcgccg gtggaccaca gcagtttaac
     3121 ctgactgccg ctgcggcccc ggaaaatgaa aatatggccg gacatgggat cgtctttcag
     3181 cgccgtttgt actttcgcag ccaggccgtt gaagctattt ctcatatcgg tgataccggc
     3241 aaccagccaa attttggtcc cggaaggtaa cgggatcatc gcttcagttc ctgtatcagc
     3301 agagtcagga gcttttcgct gacattgcca ttgaagcgga gcgtcccgtg ccggaacgtt
     3361 acctcacagc tgatactgag ggtttccggg tcctctgcga gcgattctgg ctgttcggca
     3421 gctgcatcga gagtcacagg aagtagctgg gggctctctg aagaaggtaa tagcagcttt
     3481 ccctcgcgcc attgttgtcg ccatttgaac aacagattgg cgttaatgcc attttcaaga
     3541 gcaagttttg agatggatat cccgggttca caggaggcag caacgagcag ctgtttaaat
     3601 tcgggaggat aattagggca gccttttcgc ctgccgggag tcacattttt ctgcatatct
     3661 gacactttgg ttcccactac ttatttggtg gacaccactt tgtctaattc gtcagattct
     3721 gaccagacgg ttcaggctgt acgcttacca ctattttatc cctgttgaat ttggttaagc
     3781 cggtgcagac gcgccgggaa acgcacacct gaaccggcac aataaaatac aatacaaccc
     3841 cctgttactg gagatacgaa tggaatgacg aggcagcctg tcagtcacag ggggctgttg
     3901 gcataaaatc aacggaaagg ccaccaacac cggaattaag ctccacagtc cccgcactgg
     3961 caatccattc ccgggaaagg catcaccgca aactgctccg gaggcaacca ttacagcctc
     4021 tgtaacggat tatcagccct ggcatcttcc gacggcacct cctgccgccc ccacgccctg
     4081 ctgtataccc cggtaccgca gcgtgggcag cacttttccc cctcataatc ttcgtcacac
     4141 atcacacaaa accactttct caccttcacc gtataatccg gaaccgtcat gccctgcaga
     4201 tgcaaacgac gggcctcttt ctctgccggg tcaagccagg tacatcgcag caggattttt
     4261 tcagcgacag tcatctggtc atgttcaaac cacggtggtt gtccgtcatt gccccgctga
     4321 agtgttaacg ggcatccaca ggacacacag cacaaaacgt cctgtcggtt gtgcttcgca
     4381 gcttctttcg ccgtaacaaa atggcctttg ctgtcattcg ccatataaca gttcagaatt
     4441 cgcatatttc ctcctgagtt gtccggggaa tactgaatcc tccggtctgg cagtgtgtgc
     4501 agtactgctc cccataataa tcgtggtgac attgtctgca gtgccagctg gctttacgca
     4561 ccacgggtaa ggcatccggt acgaattgct gcagacgttt aatcaactgt atttctctgc
     4621 gctccggcct gacataaggg cactgttgac cgtgctctgt cagcctgtcg tcagtgtgtt
     4681 caaaccaggg aagttcagtt tcgtattgcg gatggtatct gagtgcactg ccacacaagt
     4741 ggcaggtgta gttagcataa ggtgcggcct gtgcagtacg ggcgcccgtc agacgtccgt
     4801 tgccatcaag agcgataaag gattttgcgt acatagtcat atacctcagc cgccagacga
     4861 cacgcaggca acagccgtcc cggtacgggc agcgtggtca gggtgtgaat ggcggttcag
     4921 ttaaaatctg gactggagtt tgggtaaagc aggctggttg ctgttacagc actgtggttg
     4981 tcacacaatg gaacactgac gtaaaaaaca gtcagtgggc gagtggatac atcgttgccg
     5041 ccagacaaca acccatactt ttggtttgcc ggatattggc gcgccagtcg tgctcaactc
     5101 cggctacagc caaaattgcg ctcaccggtg cagtgagctt tggtatgctc cttcgccaga
     5161 tagtcagcac gttccagcac ttgctgaaag ccggtgtcat cattacgcgc cagccacacc
     5221 gccggccagg ccggaaaatg caccagcgtg gcatggcacc cgacatccac tcccagggca
     5281 ctgcaccacg cctgtttaat catcccggcc agagactccg gagcgcggta atcaccggca
     5341 cgacaccagg tatcccggtt gaccagcagc atcaggtgat agtgtttttt acctttgcac
     5401 tccccaaact cccgggccca gacgtaatgc agggttgtgg gatgcacgcg tttaccttcc
     5461 cgatgcttac gtttctggta agcgtcgatt cgggctttca gggcattgat gaagcgggat
     5521 atcacagctg cgtccgttgc tgccggtaca tccgggagac gcaggtcaac ccgcaatgcc
     5581 gtcaggcggg gatgaacatt cagtgcgtgc cgcaccgtct cacgaatacg ttgctgccag
     5641 aaggggttgt atttgtaggt catggttaaa tctccgtatg gttcatacgg aatagccacg
     5701 tcgtaaaaaa tacgcagagt ccctgaagtg acctccggca ggacactgtc tcaggcactc
     5761 tgtgataacc cgaacggtcg tctccggact gacggttgaa ttttcagcgt cgttttttaa
     5821 tgtaaaaaac cggttacggg taaggctgtg aggagggagt gatggtgata cgcaaaaaga
     5881 agtgcaggga ctgtggaaac gcgattactc ataatacagt gtgttgcccg tattgtggtt
     5941 ctgtcgaccc cttcggctac taccggaata cagaccgtat cgttaccata ctgctggcgc
     6001 tgattattgt ggtcctgctg actactgtct gcgtcagtgt gtgtatactg tgtagctggt
     6061 gagataacgg ttcatgtatc ccctgagctg acagtgtacc gtgtatatta agcgtaaccg
     6121 acaggttctc cttcatacca tattaaattt tccagggatt gccgctgcct tatttcgtcc
     6181 catacaggaa aactcatctg ggtttctgac aaatacgcga aactcacagc ccctcaatat
     6241 caaccacacg ttgtaagccg gtaatacctg aaaatcagcc tattaccgcc cacgcctccg
     6301 gcaccattcc cttttgattt cgaattcttt gttgtcgcta taccgcttaa tgttcccggt
     6361 cctccggtgc agataacacc ggagtcatcg tagcgctgct tcaatattca ggtgacgtat
     6421 ttccagacgg aagccggcgc gcgatcgtaa agtttgttca ttgtcagttc tgccagccgg
     6481 ttgtcaacgg cagagttaaa ggcttccagc tccgcaaggg taagtttata acgcgtgtgg
     6541 gaattcactt ttcccgcgtc tcctgacgta ttacgggctg ccacttacct ggtgtcccgc
     6601 aatacagcct tagtgttccg ttatcgggga tccggacgca ccgcaacaac cggggaagta
     6661 tttctggttt agagcagtgt caagagggat ttctcagccc ctcactcacc ggctcagtct
     6721 cagggcagga gcattaatac gctcagttca tgtcttatac aggaatgggc acagggggag
     6781 ctggcgtgtc actgctgatt ttacgcatca gcgttcatgt atctgcactc tgatacatga
     6841 acgattcaca gcgctctgtt accggagaga tttactctct tagcttactc tggcaaatcc
     6901 tttacgttac tctctgatga cttcttatat ttataatacc aatcagacag ccttatccca
     6961 tcggtgatat cagtctcctc ctcgccagga acgggctgac tcactgtaac tacctccgaa
     7021 aacaactctg caattatcag aacgcctgct tctctccctg tcctcacgaa aactatcccc
     7081 tctttatcgc gcgtgcgtgc ggaagcatct tttcgcaaca accacccggt ataccggtac
     7141 ggctcagcca ccgcaacctc cccgtttatc tcctggcggt cacattcccg attatttttt
     7201 acgtttctcc ccggttgtta tgccggtgaa ggtggcgcgc tgttttcatg accacaccgg
     7261 ctggtttaac aacatccgga ggaacattct catggctacc ccagtttcac tgatggatga
     7321 ccagatggtc gacatggcat ttatcactca actgaccggc ctgacagata agtggtttta
     7381 caggctcatc agggatgggg cctttccggc tcccatcaag ctgggccgca gctcccgctg
     7441 gctgaaaagt gaagtggaag cctggctaca ggcgcgtatt gcacagcccc gcccgtaatt
     7501 tctgaccgtt atccgttcac ccgcagcaga cgtaatcccc ggcctgccgg cggcattctg
     7561 ctgcctgtta tatccccgtg aggaatatga aaatgaaaca acagtatcag acccgctacg
     7621 aatggctcca cgaaagctac cagaaatggc tgaccggctt cacccggcac gccgtatcct
     7681 ggggcgtgtg tcatccgaat atctactact tccataatct gacgcccggt tgggtgtcat
     7741 tcaatggtga aaaaccggag attgccatcg taccccagag cctgcaccgg ctgatttatg
     7801 gccctgacaa acgggccacg ccgcccctgg atgatgatct gatagtgaat ttatgcacca
     7861 gtgaacatct gctggttcat catccgatgc tggaaggcat tctcctgtct gaatgtgaac
     7921 gtctgaggca gcgttctctg gcaaataaac tgatcagcct cttccgtcag tttggtggca
     7981 cggagttgcg cctcaaactg gtctggcttt gctggcttga tttaatgacc ggaaacagcc
     8041 ttgaagactg gaaagagaac ctgaaacgga aatcagaaaa agagctggag gaatggatca
     8101 tcaaccgtca gaggcagagc gcagcactga cggatctgat ggatcagtac gtgctcctgg
     8161 cataccgcac aacggttgac gatagccgca actgatgccg gttgcttccg gaaggtgtga
     8221 caaaatacgg aaatgatgat gctcccggcc cccatgctca cctgcagagc gtgttcgtcg
     8281 tcatcatact gagccatgac gggcgtctta agggcatcct gtcattgctg agcaggcaac
     8341 accaccagag gagccacgga cagggatgtt ctttacagcc cgatacacgc caggttctca
     8401 gcagactgaa tgtgcaggtg cggttattca ccagaaatgg catgctgcgg tacataccca
     8461 tcctggtgac aatatgtacg tggcgacagc gatcccggca ctgtggaaga gactcactca
     8521 gcctgcagga tattctttcc ggcatccggg aatgtccgcc tgaatgcaaa caggatgata
     8581 tttgaatccg taatactgtt ctttcatcat aaataattta tgcagataca gttaaccgtt
     8641 acagtagccg gtttgtttta ccgggaaata atatccgtca gactgatacc ggaacatatc
     8701 tgaaacacgt tatgatgttt tgttatcaga aatgttgtat attcttatcc gtggcgacag
     8761 tttaatgccc tctggtatca cctgataatg tatatcctgc gatgattcgt tatatttacg
     8821 ggacacgcct cttttcattg tgctcctttt cagacttcat tttccgtaag tgttccccgg
     8881 cataaaggag gtacctttct ggcgtttata actctcttta tcggtgacgc cggtaataca
     8941 gggaatacca caccgacaaa cgaggtatta acagacgata tttcagtgtc cataattttt
     9001 ctgtccggtt aaataacgtc agtctgctgc cctctgactg taatcatcaa agccggaatg
     9061 cataccgggt tacaacgtaa aaaaagtgat gctggccccc ggtgcagctg gcgggcgaaa
     9121 catgtttacc tttcaatacc ccctgtctgc agtctccact cttccatcct cttaagctac
     9181 gtcggccaca tgagtgcact tccatgataa caggtattcc tgtcagcata acgaagccgg
     9241 ttaccttccc ttcctcctgc atttcctgtc agaatgacta tacagcgcgc agattgcctt
     9301 tttattgacc atcttaatat accgttataa acttcttttt atcatattat tatcacctta
     9361 ttatgtttaa tttttaagca gtcggagaaa gaggatggtt cagaaaattt tgtcggataa
     9421 ggttatgaat gaaagaacaa atgcgtatta ctcttattat cttggagagc gtaatatctc
     9481 cgtactgccc cttaatgttt atgatccccc ggaacgtttt atcgcttaca taaaaaaaaa
     9541 cagagaaaat ctgaacatca cactttcaga ttttgaactt gaacaaataa tttctggtat
     9601 gcgtctgaag gccctggcct tcctggtccc cctggaaaaa atatcctgga tcgcgggcag
     9661 tgaacgcgca tgcttatttt cctggtatct gttgatgcag tttatccaga ataacagggc
     9721 aaaaataagc gcagacttgc ttcaaaagaa taaactgtat cttaaagaag agtatctgga
     9781 agggaatgcc ttcccgtcgg actcatcgac acagttcaga caaatactca gagttctcga
     9841 tattctttct gataagaatt tgcgggatga gtggataatc cagaccaaag acagatggat
     9901 gcgggcattt aaatcaaaga gtcctttcag ttacctcctc ccggagaacg aacatgaatg
     9961 catatggaca tggaattatt tgaagggaaa aaatattgca ctggaaaaac tggccagttt
    10021 tcctggttct gctgatattt accacgcaat tcacctttca tttgacatat gggttacctg
    10081 tccactcacc tcacctgacg atataaaaaa tttcagaaac agtttcaata aggccaaggc
    10141 gcagcgcaag tacaaaaaaa tgcaggaaga taaagtcaat gtacagtttt ttcttgacgc
    10201 agaaaccagg gcgcagctca aagaattatc gagggtcaga agactcagta ccggcgaaat
    10261 gctgcatgac ctgattgttg aagagtataa aaggtaccga c
//
LOCUS       DAJVOY010000145        10205 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVOY010000145 DAJVOY010000000
VERSION     DAJVOY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.145"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..883)
                     /gene="gapA"
                     /locus_tag="OQL90_003179"
     CDS             complement(<1..883)
                     /gene="gapA"
                     /locus_tag="OQL90_003179"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1369855.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFD"
     gene            1225..1638
                     /gene="msrB"
                     /locus_tag="OQL90_003180"
     CDS             1225..1638
                     /gene="msrB"
                     /locus_tag="OQL90_003180"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1369856.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1680..1952
                     /locus_tag="OQL90_003181"
     CDS             1680..1952
                     /locus_tag="OQL90_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1369857.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2113..2754)
                     /gene="pncA"
                     /locus_tag="OQL90_003182"
     CDS             complement(2113..2754)
                     /gene="pncA"
                     /locus_tag="OQL90_003182"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1369858.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2765..3781)
                     /gene="ansA"
                     /locus_tag="OQL90_003183"
     CDS             complement(2765..3781)
                     /gene="ansA"
                     /locus_tag="OQL90_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1369859.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(3948..5804)
                     /gene="sppA"
                     /locus_tag="OQL90_003184"
     CDS             complement(3948..5804)
                     /gene="sppA"
                     /locus_tag="OQL90_003184"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1369860.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5965..6516
                     /locus_tag="OQL90_003185"
     CDS             5965..6516
                     /locus_tag="OQL90_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1369861.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAILEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6633..7676
                     /gene="selD"
                     /locus_tag="OQL90_003186"
     CDS             6633..7676
                     /gene="selD"
                     /locus_tag="OQL90_003186"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1369862.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQACVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7681..9642
                     /gene="topB"
                     /locus_tag="OQL90_003187"
     CDS             7681..9642
                     /gene="topB"
                     /locus_tag="OQL90_003187"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1369863.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            9769..>10205
                     /locus_tag="OQL90_003188"
     CDS             9769..>10205
                     /locus_tag="OQL90_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369864.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLITPYRFYHKQWSETLEFWPRKPEPGK"
BASE COUNT         2572 a         2498 c         2699 g         2436 t
ORIGIN      
        1 catcgaacac ggaagtgcaa acttcgccgt tgaaatcggt agatactacg tcatcttcgg
       61 tgtagcccag aacgcctttc atttcgcctt cagcagcggc tttaacggca gctttgatct
      121 gctcgtaagt tgcagctttt tccagacgaa cggtcaggtc aactacggat acgttcgggg
      181 tcggaacgcg gaacgccata ccagtcagtt tgccattcag ttctggcagt actttaccta
      241 cagctttagc agcaccggta gaggacggga tgatgttctg ggaagcgccg cggccgccgc
      301 gccagtcttt gtgagacggg ccatcaacgg ttttctgagt agcggtagta gcgtgaacgg
      361 tggtcatcag accttcgatg atgccgaagt tatcgttgat aactttagcc agcggagcca
      421 ggcagttggt ggtgcaggaa gcgttggaaa cgatgtcctg gccagcatat ttgtcgaagt
      481 tagcgccttt aacgaacatc ggagtgttgt ctttagacgg accagtcata accactttct
      541 tcgcaccagc ggtgatgtgt ttacgagcag tttcgtcagt caggaacaga ccagttgctt
      601 cagcgacaac gtcaacacca acttcgtccc atttcaggtt agccggatca cgttcagcgg
      661 taacacggat ttttttaccg ttaacgatca gatgaccgtc tttcacttca acggtaccgt
      721 cgaaacggcc gtgagtggag tcatatttca gcatgtatgc catgtaatca gcgtctaaca
      781 ggtcgttgat tgcaacgatc tcgatgtcag aacgtttctg agcagcacgg aaaacaatgc
      841 gaccgatacg gccaaaaccg ttgataccta ctttgatagt catatattcc accagctatt
      901 tgttagtgaa taaaaggttg cctgtaaaat tacaaaaacc ttacgcagcg tcaagcggaa
      961 tcgtgtcaat cattgcgaca aatcaatcct gtgcctaagc attacgcgac tgactcgcct
     1021 cactcttcct ttgggcttga aaccacatgg ggtcggcgcc ccgaatttta aagggcaatt
     1081 acgataaaaa tgtgattttc atcacgattt aggtgcagcc atttcgtcga gatcaagttt
     1141 aggacaaaat tttgccagtt ggtgttaatg ttttgttaga atcagtcacg taatgtgagc
     1201 acgattaaag tgagatgtga gcaaatggct aataaacctt cggcagaaga actgaaaaaa
     1261 aatttgtccg agatgcagtt ttacgtgacg cagaatcatg ggacagagcc gccatttacg
     1321 gggcgtttac tgcataacaa gcgtgacggc gtatatcact gtttgatctg cgatgccccg
     1381 ctgtttcatt ccgaaaccaa gtatgattcc ggctgtggct ggcccagttt ctacgaaccg
     1441 gtgagtgaag aatccattcg ttatatcaaa gacttgtcac atggaatgca gcgcatagaa
     1501 attcgttgcg gtaactgtga tgcccatctg gggcatgtct ttcccgacgg gccgcaacca
     1561 acaggcgaac gttattgtgt taactctgct tctttacgct tcactgatgg cgaaaacggc
     1621 gaagaaatca acggttgaaa aaacgattca gcaacttatt ccacaggagc ggaaaaatta
     1681 tgaatcttga tgacattatc aacagcatga cgcctgaggt ataccagcgt ttgtcgaccg
     1741 ccgttgaact ggggaaatgg cctgatggcg ttgcgttgac cgaggaacaa aaagagaatt
     1801 gcctgcaact ggtgatgctg tggcaagccc gccataatac cgaagcacag catatgacaa
     1861 ttgacacaaa tggtcagatg gtcatgaaga gcaaacagca gttaaaagaa gattttggta
     1921 tctcagcaaa gcctattgct atgtttaagt gaatgagtta tctagtattt aagcctgtta
     1981 ttaagggagg gcaaatatac ttgttcggac gtcgtgtttt agctgcaagt aggttaaatg
     2041 caacactgac aagtattatc tggcctacaa tgtcattgag gaaatgtagg ccagagatga
     2101 attcaactaa tattacccct gtgtctcttc ccagtctgcc agcgtatata gcgttgcccc
     2161 agctgctgac atctccataa acgcgtgtgc actgtcctgg ggctggatat tcacgccacg
     2221 acaaccatcg gtaatcacgt tcaccttata acctaactgt aacgcgtcca gcacggtaaa
     2281 cttcacgcaa tagtcagtag ccaggcccat aacgatcaat tcatcgattt catgatcgcg
     2341 taaccagtca tcgagagagg ttttctgccg acggccgtta tcaaaaaagg cactgtaact
     2401 gtcaactaaa ggattttcgc ctttatggaa caccgctgcg atcgcttttt ggttcagtaa
     2461 cggatgtaat tgtgcgcctt cactgttctg cacacagtga tctggccaga aggtttgtgg
     2521 caaaccgtcg agttggcctg gcgtataagg ctctacaccg tgctgactgg caaaactgcc
     2581 gtgattcgcc ggatgccagt cctggctggc gataaccgct tctccgcgcg actggcacca
     2641 gtcaataagg cggttagcaa cctccaccgt actgtcacct tccggtacgg cgagcgcgcc
     2701 accagcacag aaatcatttt gtaaatcgac cagtaacagg gcgcgatggg gcattgcagt
     2761 ctccttaatc atccggcgtc agttcgccgc gcaggttttg gctcatggcc ttgcgaatgg
     2821 tttcagtatc cagttcctgg ctcagcaggt aatgcagttt ggttagcgtg gcttctacag
     2881 tcatatctgc accgccaata acgccggcat gggcgagggc gttaccggtg gcataaccac
     2941 ccatattcac tttaccggac atacattgtg tcaggttgac caccacaata ccgcgatcgc
     3001 tggcttcttg taattcctgc aggaaggctt tgttttgtgg cgcattaccc acgccatagg
     3061 agcgcagaat caatgctttc actggttggc gcagaaaatt gcgcacgaca tcagcagaaa
     3121 tccccggata aatcgtcact acgccaattg gttgtggcgt gattggatga acgattaatt
     3181 ccccttcacc gtgcggggcg ggtggcgtat tcaaacgacg aatatggata ccagcttcca
     3241 gtaacggagg aaggtttgga gaggcaaacg catcaaaacc atcggcatgg gctttggtag
     3301 tgcggttgcc gcgatacaat cggttattga aaaagagcgt tacttcgttg atcggataat
     3361 tcgccgcaac gtacaacgca ttcagcagat taatttgtcc gtcagagcgt aactcagcca
     3421 gcgggatttg tgaccctgtc acaatgaccg gtttaccgag attctcgagc atgaacgaca
     3481 gcgcagaggc ggtatacgcc atcgtgtcgg taccgtgtag aatgacaaaa ccatcatagt
     3541 cgtcatagtg cgctttaata tcttcagcaa tatgctgcca gtcatccggc gtcatatcag
     3601 aagaatccat caacggcgta tattcatgaa tggtgaaatc tggcatctcc gggcgatgga
     3661 attccggcat cagcgccagt tggcgttgta gatgacctga caccggaata taaccctgct
     3721 cggaacgctg catcccgatg gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca
     3781 tgatattgat atgaatgata tcgaaagagc aaaagtatat acgtgaggca ggaggagcgc
     3841 acctgatact atgcggataa ggaaatatgg ggcgagacag cgggaagagg attcgtcgag
     3901 gtaaaaaata atgtggccga cgatctgtcg gccactcagt acaagactta acgcacgttg
     3961 gcgcaggtca ggcaaaacgc ataacggttt tgtgggtcgt taaacgcggc cagcttgtcg
     4021 ctttcacttt taacggtaga ggctaccgag gcaagcggtg caggtaacat ggcctggaac
     4081 gcatctggca acattgcccg gacagaacca gacatgttgt ccatcacttt gtcgaagaag
     4141 gtcggttcat caacgtagta ttccagatgc cactgtttca cttttgcgag ctctgctgct
     4201 ttggcaaccg catcatcgaa atccccgaga ctatcgacca gcccgttagc ttttgcatcc
     4261 tgaccggtcc agacgtggcc ctgggcgatt ttatcaattt gctccggcgt cgaatgacgc
     4321 gcatcagcaa ccagcgtgat aaagcgttta tagccattct caatgcttaa ttgcatcatc
     4381 tgctgcgctt ccggcggcag tgccctggtg atagaaacat ccgccagcgg tgaagttgag
     4441 acaccatcag tatgaacacc aatcgaatcc agactatttt ctacggtggt gatcacgccg
     4501 aagataccga tagaaccggt cagggtgctg gggttagcca caatgtaatt agctggcgtg
     4561 gaaatccagt aaccaccaga tgccgccatg ccgcccatcg atacaaccac aggcttaccc
     4621 gctgcccggg ctgctgccag ttcagcgcga atcacttcag acgcggtaac gctgccgcct
     4681 gggctattaa cacgcaggac aatcgctttc actttcgggt caaggcgagc gtcgcggatt
     4741 tgtgccgcag tggtatcacc gccaacattc ccctgagttt cctcgccatc cataattgcg
     4801 ccattagcga agacgacacc gatgctgtca ccggtatctg ccggcgtttt caatgcgtaa
     4861 tcgtaataac tgatggcgcg ataattttta tcagtcttac tccagccgaa ctctttggtc
     4921 agagcttttt cgatttcggc actcgatgcc agtgcatcga ccagcttgtt ttccagtgca
     4981 tatttcgcgg tatcgccacc ggttttggtt aaaccctcaa gcaacccttg cgcgctaggg
     5041 aatacctgct gagcagggat ctgtcggtta gcggcaacag tattcagata gttttgccac
     5101 agctcaccaa tccagcggct gtcagcttcg cgggctgccg gtgacatatc atcacgaata
     5161 aacggttcaa cggcagattt atacgtaccc acgcggaaca catgggtgga aactttcagc
     5221 ttatccagca acgatttgta gtacagaccg ttggtagcaa aaccgtgcag atcaaccacg
     5281 ccttgcggtg acagccaaat tttattggcg aaactggcga gataatattg cccctggctg
     5341 tagttctcgc caacggcata aaccggtttc ccgctgtcac gaaactcttt cagggctttg
     5401 ccgatgtact gcatagacgg ctggtcgccg cctgcgaagt ttttcagatc catcacgata
     5461 ccggtgatat tgtggtcgtc cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag
     5521 ttttcctgca gacgatcgga actggcacca agcagctggc ggcttaattt actaaaccgc
     5581 tgagaactgt cgggtttatc gacgatcaca ccggaaatgt ccagcagcag tgcgccacga
     5641 ctggccgttt ctttcgaatc accaccactg acctgcatcc agatccccac accaaccagt
     5701 acgaggaaaa taaagaacag gttaagtacc atttcacgga cgaaattcag cagacgccac
     5761 gtccatttaa aaaatccggc aataaatcgc caaagggttc gcatgtattc tcccaactta
     5821 aggtcacacc tgtcacaggc gcaatagatg tggttatcgt aatgacccaa ccgacacttg
     5881 tcagcaggaa ttgcggactg tgctgtaaca aaatccactc tcgtgttaat tttgtgaata
     5941 aatatcacga caggagttaa tcaaatggat gcactcgaac tattgatcaa tcgccgtagc
     6001 gcctcccgcc tggctgaacc cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg
     6061 ggtatgcgtg cgccggacca taagtccatg caaccgtggc atttttttgt gattgaaggg
     6121 gaagggcgcg agcgtttcag cgccatactg gaacaggggg cgattgctgc cggtagtgat
     6181 gacaaagcta tcgacaaagc ccgtaatgcg ccgttccgcg caccgctcat catcacggtg
     6241 gtggcgaaat gcgaagaaaa tcataaagtc ccgcgatggg aacaggaaat gtctgccgga
     6301 tgcgcggtca tggcgatgca aatggcagcc gttgcccagg gatttggcgg catctggcgc
     6361 agtggcgcat taactgaaag tccggtagtg cgtgaagcat tcggttgccg tgagcaggat
     6421 aaaattgtcg gttttctcta cctcggcacg ccgcagttga aagcatctac gtcgattaac
     6481 gtcccggacc cgacgccgtt tgtaacttat ttctgataat tgtcgcgaaa ctgtctggat
     6541 tgtgagcaaa ggcgcaggaa ttcagacact ctcacttatc acttcacgga atgagggtta
     6601 ccatacccgg attgcattga caggagatgt ccatgagcga gaactcgatt cgtttgaccc
     6661 aatacagcca cggagctggt tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc
     6721 tgcacagtga gcaggcgaag tttgttgatc cgaatttgct tgtgggtaat gagacccgcg
     6781 acgatgcggc ggtgtacgat ctgggcaatg gcaccagcgt tatcagtacc accgacttct
     6841 ttatgccgat cgttgataat cctttcgatt ttggccgcat tgcggcgact aacgccatca
     6901 gcgatatctt cgcgatgggg ggcaaaccga ttatggcgat tgcgatcctc ggctggccga
     6961 ttaacaaact ttccccggaa attgcccgcg aagtaaccga aggtggacgc tatgcatgcc
     7021 gtcaggcggg tattgcgctg gctggcggtc actccatcga tgcgccggag ccgatttttg
     7081 gtctggcggt aacggggatc gtaccgaccg agcgggtgaa gaaaaacagc accgcacaag
     7141 ccggatgcaa actgttcctg acgaaaccgc tggggatcgg cgttcttacc acggctgaga
     7201 aaaaatcact gctgaaacca gaacatcagg gactagcgac ggaagtgatg tgccggatga
     7261 acatcgcagg cgcgtccttt gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg
     7321 gctttggtct gctgggccat ttgagcgaaa tgtgtcaggg cgcaggtgtg caggcgtgcg
     7381 ttgactatga cgcgatccca aaattgccgg gggttgaaga gtacattaag ttgggcgcag
     7441 tgcctggcgg caccgaacgt aactttgcca gctacggtca tctgatgggt gaaatgccgc
     7501 gtgaagtgcg cgatttgctg tgcgatccgc aaacttctgg cggtttgctg ctggcggtca
     7561 tgccggaagc agaaaatgag gtcaaagcta cagccgccga gtttggcatt gaactgacgg
     7621 caattggcga actggtgcca gcgcgcggcg gtcgtgccat ggttgagatt cgttaattca
     7681 atgcggttgt ttattgccga aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc
     7741 aaaccgcacc ggaaaggcga tggctttatc gagtgcggta atggtcaggt ggtgacctgg
     7801 tgtatcggtc acctgcttga gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc
     7861 tggaatcttg cggatttgcc gattgtcccg gaaaagtggc aattacagcc ccgaccctcc
     7921 gtgaccaaac aacttaacgt catcaaacgc ttcctgcatg aagccagcga aatcgttcac
     7981 gccggggacc cggatcgtga agggcaactg ctggtggatg aagtgctgga ctatctgcaa
     8041 ctggcaccgg aaaagcgcca gcaggtgcag cgttgcttga taaacgacct gaacccgcag
     8101 gcggttgagc gggcgatcga ccgtcttcgg tccaacagtg agtttgtgcc attgtgcgtt
     8161 tctgcgctgg cgcgagcgcg tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat
     8221 accattctcg gtcgcaatgc cggttatcag ggcgtacttt ccgtgggacg cgtgcagaca
     8281 cccgtgctcg ggctggtggt gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac
     8341 ttctttgaag tcaaagcaca tatcgtgaca cctgccgatg agcggtttac cgctatctgg
     8401 caaccgagcg aagcgtgtga accgtaccag gatgaagaag ggcgcttgtt acatcgtcca
     8461 ctggcggagc atgtggttaa ccgcattagt ggtcaaccag caattgtcac cagctataac
     8521 gataaacggg aatcagaatc cgcgccgctg cctttttcgc tttcggcgtt gcagattgaa
     8581 gcggcaaaac gctttggtct gagcgcgcag aacgtgcttg atatctgcca gaagctgtac
     8641 gaaacgcaca agctaatcac ttatccgcgt tccgattgtc gctatttgcc agaagaacat
     8701 tttgccggac gccacgcggt gatgaatgcc atcagcgttc atgcaccaga tctgttgccg
     8761 caaccagtgg tagatccaga tatacgcaac cgctgttggg atgacaaaaa ggtcgatgca
     8821 caccacgcca tcatcccgac cgcacggagt tctgcgatca acctgacgga gaacgaagcg
     8881 aaggtctata acctgattgc ccgtcagtac ttgatgcagt tctgcccgga tgcggtgttc
     8941 cgcaagtgtg ttatcgaact ggacattgcc aaaggcaaat ttgtcgctaa agcgcgtttt
     9001 cttgctgaag caggctggcg cacgctgtta ggcagcaaag agcgcgatga agaaaacgac
     9061 ggtacgccat tgcctgtggt ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg
     9121 gtagagcggc aaacccagcc gccgcgccat tttaccgatg caacactgct ttcggcgatg
     9181 accgggatcg cgcgctttgt gcaggacaaa gatctgaaaa agatccttcg tgcgaccgat
     9241 ggtctgggga cagaagcaac ccgtgccggg attattgaac tgctgttcaa gcgtggtttt
     9301 ctgaccaaaa aagggcgcta tatccactct accgacgccg gaaaagcgtt attccattcg
     9361 ctgccagaaa tggcgacgcg accggacatg accgcgcact gggaatcggt gctgacgcaa
     9421 atcagcgaaa agcagtgtcg ctatcaggac tttatgcagc cgctggtggg gacgctatat
     9481 cagcttattg atcaagccaa acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg
     9541 ggcagtggtg gcagtgctga taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa
     9601 agtccgccag cagatgaagt cggaagcggg gcgatagcgt aagcgagtga atctttcgtg
     9661 ctattcgagt catattctga aatatccagc gaatcaagaa aattcgttgg atattttttt
     9721 gcatggataa aattatcgcc tctaaagtat gtaataacag ggaatgtgat gaaaaaagtt
     9781 ttattacaaa accatcctgg gagcgagaag tattctttta atggctggga aatatttaat
     9841 agtaattttg aacggatgat taaagaaaat aaggccatgc tgctttgtaa gtgggggttt
     9901 tatttaacat gtgttgtcgc tgtaatgttt gtgttcgcag cgataacatc caacggtttg
     9961 aatgaaagag gcctgattac cgcgggatgc tcttttcttt atctattaat tatgatgggg
    10021 cttattgttc gggccggttt taaagcaaaa aaagaacaac tgcattatta tcaggctaaa
    10081 ggtattgagc cgctcagtat cgagaagtta caggcgctac aattgatcac accttatcga
    10141 ttctatcata agcaatggtc tgaaacgctg gagttctggc cgcgaaagcc tgaacctggc
    10201 aaaga
//
LOCUS       DAJVOY010000146        10205 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVOY010000146 DAJVOY010000000
VERSION     DAJVOY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.146"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OQL90_003189"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OQL90_003189"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1369865.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="OQL90_003190"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="OQL90_003190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1369866.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="OQL90_003191"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="OQL90_003191"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1369867.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="OQL90_003192"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="OQL90_003192"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1369868.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="OQL90_003193"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="OQL90_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1369869.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="OQL90_003194"
                     /pseudo
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="OQL90_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            5724..5885
                     /locus_tag="OQL90_003195"
     CDS             5724..5885
                     /locus_tag="OQL90_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1369870.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="OQL90_003196"
     CDS             6007..7080
                     /locus_tag="OQL90_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1369871.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="OQL90_003197"
     CDS             7077..7859
                     /locus_tag="OQL90_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1369872.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8073..8936)
                     /locus_tag="OQL90_003198"
     CDS             complement(8073..8936)
                     /locus_tag="OQL90_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1369873.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVGSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8908..>10205)
                     /locus_tag="OQL90_003199"
     CDS             complement(8908..>10205)
                     /locus_tag="OQL90_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1369874.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
BASE COUNT         2410 a         2667 c         2749 g         2379 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt tacgccgcat
      241 ccagcaaggc ttgcggcggg atacttaacc ttttttcgcc tggcgggtca taatggcgcg
      301 atataccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tttcatccag
      361 cacgtccgga taaggcagga aggcgttcgc tacaattcgc agctcgccgc cactattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc gtatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa aactttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgtg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctgaaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcttc
     2101 aaacgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gtttcgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgctaaaatc
     2581 ggcatcatca ccaacggttt tagcgccttg cagcaagtgc gtctggaacg gacgggcctg
     2641 cgtgattact ttgatttgct ggtgatttcc gaagaagttg gcattgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaactggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacttgc
     2821 tggctgaatg cgcaccatct cgagcaacca gaaggcatcg cgcccacctg gaccgtttca
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggacgc cggtaaaact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgctgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgcta gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac agcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctttatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga actgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggcccct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaatatg gacccgaaac atcgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 aacgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cacgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcggccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgag caaaaagtcg tgaccctgag cggtttcgtt gaaagctagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctcggtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtagat tctcaggcac
     5521 aaagtgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtt tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgtaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggttg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgattg acgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgacacc cactgccatt ttgatttcct gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgt gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattgggctt gcatccaggt atgttggaaa aacatagcga tgtgtctctt gagcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcagt gggggaggtc ggtctggatc
     7381 tcttcggcga cgatccgcaa cttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc tgcattcacg gcgcacacac gacaaactgg
     7501 cgatgcatct taaacgccac gatttatcgc gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagt
     7861 ttacccttcg taggcagtat ccggcagtcg gcgcataatg cctgatgcga cgcttgtcgc
     7921 gtcttatcat gcctacaggt ttgtgccgaa ccgtaggccg gataaggcgt tcacgccgca
     7981 tccggcagtt ggcgcacaat gcctgatgcg acgcttgtcg cgtcttatca tgcctacaag
     8041 tctgtgccga accgtaggcc gtatccggca tgtcacaaat agagcgccgg aaatatcaac
     8101 cggctcaccc cgcgcacctt taacgcatca gccaacggct caacgtcttc cggcgtggcg
     8161 ctcgcccagc tttgcgcctc gccatacacg ccgtgggcat gaaacgcatt caggcgaacc
     8221 ggaacatcgc caagtctctt gataaacgtc gccagctcat cgatgtgatg caaataatcc
     8281 acctggtctg gaattaccag caaacgcagt tccgccagct tgccgcgctc tgcaagcaaa
     8341 cagatgctgc gcttaatctg ctgattatcg cgtccggtta agtgttgatg acattcgctc
     8401 ccccacgctt tgagatcgag cattgcgccg tcacacaccg ggagtaattt ttcccagccg
     8461 gtttcgctca acatgccgtt actgcccacc aggcaggtga ggtggcgcag ttgcggatcg
     8521 tttttgatag cagtaaacag cgccactaca aacggcagtt gggtcgtggc ttcgccgccg
     8581 cttaccgtta tcccttcaat aaacagcacc gctttgcgga catggctaag cacttcgtcc
     8641 acgctcatgg attgcgccat tggcgtggca tgttgcggac acatcttcag gcaggtatca
     8701 cactgctcgc agaccacagc gttccacagc actttgccgt caacaatctg caacgcctga
     8761 tgcggacact gtggcacgca ctctccgcaa tcattgcagc gtcccatcgt ccacggattg
     8821 tgacagtttt tgcagcgcag attgcagccc tgcagaaaca gagccagacg actgcctggc
     8881 ccgtcaacgc aggagaaggg gataatctta ctgactaaag cgcatctgct gttcatggct
     8941 tatcacgcgc ggctggcgtt ccagaatacg agtgttgcgt gtggcttctt cgcccagcca
     9001 ggtggtgttg gtgcgtgaac cttcggcgcg atatttttct aaatccgaca aacgcaccat
     9061 ataaccggta acgcgaacca gatcgttacc gctgacattg gcggtaaatt cacgcattcc
     9121 ggctttaaag gcaccgaggc aaagctgtac cagcgcctgc ggattacgtt tgatggtttc
     9181 gtcgagcgtc agaatgtcgc tgatgccgga ataataataa gcatgatgcg gggcgacagt
     9241 ttgcagatgg atgatcggat ctggctcatc gccatacggc aaacgcgcgc ccggcgtggt
     9301 gccgatatcg gaactgatcc ccgactgtgc gtgtaacatg gcgcgttttt gccagccata
     9361 tttcacgggg gtattggcga caaactccgc cagttgcgcg ctgatgcgat aacccacttc
     9421 atttgcggcg gcttctttac cgtagcgcgc ggcaatccct tctttctcac acagcaagtt
     9481 aaccgcttcc gccagcccat acatgccaaa cattggcaca aaacgttcag ggttaatcag
     9541 cccttctttc accaggaagc tattctcaaa gaagtgtgat tgttgataga ggaattcaca
     9601 ccgcgcatcg atgatggcga tctgctgctg gcagtagtgc ggtagagtgc gcgtaaagaa
     9661 gtcatccagc gattcgctgc gctcggcaat ggctttcagg ttaaggcgta ccagcgtgct
     9721 gccaccaccc gccagcggca gtgagttgta acagctcaca atcccgtagc ccccttttgt
     9781 gaaaatttta tcatgcaccg gaccgttggc gatgtgcggt ttgctacatt cacagatgtt
     9841 cttcgccact tccagcagca ggtcatcagg ggggatttca ggatcgtaga taaaggtcag
     9901 gttcggtgaa acctgcttca actctgcgtc tgcacgtaag atcgcgcggg taatgggcga
     9961 atcagacggg ccgatattgg cgtgcataaa ggcgtctggc agggttccgt cgaggtaacg
    10021 ccagaaacgt tttattcgaa catcgatctc gtcttgtgtt agaattctaa catacggttg
    10081 caacaacgca tccagttgcc ctaggtagac cggcatcgat gtgaccgacg gtacgtggtg
    10141 gtaaagaatg gtcagcagag agagtgcgtc atcaagatct ttcgcgcctt ccagctccag
    10201 ccatt
//
LOCUS       DAJVOY010000147        10203 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVOY010000147 DAJVOY010000000
VERSION     DAJVOY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.147"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            131..1987
                     /gene="kup"
                     /locus_tag="OQL90_003200"
     CDS             131..1987
                     /gene="kup"
                     /locus_tag="OQL90_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS1369875.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSLTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2154..2573
                     /gene="rbsD"
                     /locus_tag="OQL90_003201"
     CDS             2154..2573
                     /gene="rbsD"
                     /locus_tag="OQL90_003201"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1369876.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2581..4050
                     /gene="rbsA"
                     /locus_tag="OQL90_003202"
                     /pseudo
     CDS             2581..4050
                     /gene="rbsA"
                     /locus_tag="OQL90_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4055..5020
                     /gene="rbsC"
                     /locus_tag="OQL90_003203"
     CDS             4055..5020
                     /gene="rbsC"
                     /locus_tag="OQL90_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1369877.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5045..5931
                     /gene="rbsB"
                     /locus_tag="OQL90_003204"
                     /pseudo
     CDS             5045..5931
                     /gene="rbsB"
                     /locus_tag="OQL90_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6057..6986
                     /gene="rbsK"
                     /locus_tag="OQL90_003205"
     CDS             6057..6986
                     /gene="rbsK"
                     /locus_tag="OQL90_003205"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1369878.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            6990..7982
                     /gene="rbsR"
                     /locus_tag="OQL90_003206"
     CDS             6990..7982
                     /gene="rbsR"
                     /locus_tag="OQL90_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1369879.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSYPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7948..9375)
                     /gene="mdtD"
                     /locus_tag="OQL90_003207"
     CDS             complement(7948..9375)
                     /gene="mdtD"
                     /locus_tag="OQL90_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1369880.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     RATWHWIFLINIPIGIAGLLYARKHMPNFTTAQRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9398..10090)
                     /locus_tag="OQL90_003208"
     CDS             complement(9398..10090)
                     /locus_tag="OQL90_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1369881.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2483 a         2457 c         2696 g         2567 t
ORIGIN      
        1 tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc cgttaatcgt
       61 gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata tttcaacgaa
      121 agactagtct atgagcactg ataataagca atcattgccc gcgattaccc tcgcggcgat
      181 tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg aatgtttgtc
      241 cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat cgctgatctt
      301 ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc gggcagataa
      361 cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata cgtcggcgcg
      421 aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct atggtgaagt
      481 cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa tcgtcgcccc
      541 gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat tatttatgat
      601 tcaaaaacat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc tgacctggtt
      661 tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag tgctgcatgc
      721 gctgaatcca atgtgggcgg tgcatttctt ccttgaatac aaaacggttt cttttattgc
      781 attaggggca gtggtgttgt cgattacggg ggtcgaggcg ctgtatgctg atatggggca
      841 ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt ccttgaccct
      901 taattacttc ggtcagggag cgctgttgtt aaagaacccg gaagcgatta agaacccgtt
      961 cttcctgttg gcaccggact gggcgctgat cccgctgctg atcatcgccg cactggcgac
     1021 ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc aggcggtacg
     1081 tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt cagggcaaat
     1141 ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga ttgtcagctt
     1201 tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa ccatggtgct
     1261 gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata agtattttgt
     1321 tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg ctaacctcga
     1381 taaactgctc tccggcggtt ggttgccatt gagcctcggt acggtgatgt ttatcgtgat
     1441 gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac atggtaactc
     1501 tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc ccgggaccgc
     1561 ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata accttaaaca
     1621 taacaaggta ttgcatgagc gggtgattct gttaactctg cgtaccgaag acgcgccata
     1681 tgtccataat gtccgtcggg tacagattga acaactgtcg ctcactttct ggcgcgtggt
     1741 ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc gctgcggtct
     1801 ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg agtcgaaacg
     1861 cccgtggtat ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta atgcgctgcg
     1921 tgcaccagat caatttgaaa tcccgccaaa cagggttatc gaactgggta ctcaggtcga
     1981 aatctaacgc cagacgcctc ctttctccat aagggggcgt ttttgttttc atggttaatc
     2041 accatgtaaa acgtttcgag gttgatcaca tttccgtaac gtcacgatgg ttttcccaac
     2101 tcagtcagga ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa aaaatgaaaa
     2161 aaggcaccgt tcttaattct gatatttcat cggtgatctc ccgtctggga cataccgata
     2221 cgctggtggt gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt atcgatatgg
     2281 cattaaccca gggtgtacct tcttttatgc aggtgctggg cgtcgttaca aatgaaatgc
     2341 aggtcgaggc tgccattatc gcggaagaga tcaaacaaca taatccgcaa ctccacgaaa
     2401 cgttgctcac tcaccttgag cagctgcaaa aacaccaggg aaataccatt gaaattcgtt
     2461 acaccacgca tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta attcgcagcg
     2521 gagaatgttc tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc tgaggccgtc
     2581 atggaagcat tacttcagct taaaggcatc gataaagcct tcccgggcgt aaaagccctc
     2641 tcgggcgctg cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg cgaaaacggc
     2701 gcgggtaaat ccaccatgat gaaagtgctt actggtatct atgctcgcga tgccggcacg
     2761 cttttatggc tggggaaaga aacgacattt accgggccga aatcttccca ggaagccggg
     2821 attgggatta tccatcagga actgaacctg atcccgcagt tgaccattgc cgaaaacatt
     2881 ttcctcggtc gtgagtttgt taatcgcttt ggcaaaattg actggaaaac catgtatgcc
     2941 gaagcggata aattgctggc taaacttaac ctgcgcttta aaagcgacaa gctggtgggc
     3001 gatctttcca tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag ctttgagtcg
     3061 aaagtcatca ttatggatga accgaccgat gcgctgaccg ataccgaaac cgaatccctg
     3121 ttccgcgtca tccgcgaact aaaatcgcaa ggccgcggta ttgtctatat ctcccaccgc
     3181 atgaaagaaa tcttcgagat ttgcgatgcc gttaccgttt ttcgtgacgg gcaatttatt
     3241 gctgagcgcg aagtggcatc actgaccgaa gattcgctga ttgagatgat ggtgggtcgc
     3301 aagctggaag atcaatatcc acacctggac aaagcgccgg gagatatccg cctgaaagtc
     3361 gataatctct gcggacctgg cgttaacgat gtctctttta ctttacgcaa aggcgaaatt
     3421 cttggcgtct ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt gctctacggc
     3481 gcactaccgc gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt tacccgttca
     3541 ccgcaggatg gcctggcaaa cggcattgtg tatatctccg aagaccgtaa acgtgacggt
     3601 ttagtgttgg gcatgtcagt aaaagagaac atgtcgctga cagcgctgcg ctacttcagc
     3661 cgcgctggcg gcagtttgaa gcatgccgat gaacagtagg ctgtgagtga tttcattcgt
     3721 ctgtttaatg tgaaaacacc gtcgatggaa caggcaattg gtctgctttc cggtggcaat
     3781 cagcaaaaag tgttgatcct tgatgaacct acccgtggcg tagatgtcgg cgcgaaaaaa
     3841 gagatctatc aactgattaa ccagttcaaa gccgatggct tgagcatcat tctggtgtca
     3901 tcggagatgc cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat
     3961 ctcagcgggg aatttactcg tgagcaggcc acccaggaag tgttaatggc tgccgctgtg
     4021 ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca acccagactg tctctggtcg
     4081 ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct
     4141 gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat
     4201 tctccagcaa acctcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac
     4261 ttcgggcatc gacttgtctg taggttctct gttggcgcta accggcgcag ttgctgcatc
     4321 tatcgtcggc attgaagtca atgcgctggt ggctgtcgct gctgctctcg cgttaggtgc
     4381 cgcaattggt gcggtaaccg gggtgattgt agcgaaaggt cgcgttcagg cgtttatcgc
     4441 tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc
     4501 agtgaatacc ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc
     4561 gctgggcgta ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat
     4621 gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac
     4681 gcgtctttct ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct
     4741 gctggcatcg ctggccggga ccattgaagt ggcgcgtctc tcctccgcac aacccacggc
     4801 ggggactggc tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc
     4861 gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa
     4921 taatggattg aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt
     4981 gattttgctg gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt
     5041 gaatatgaac atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt
     5101 cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc
     5161 gttctttgta tcgctgaaag atggcgcgca gaaagaggcg gataagcttg gctataacct
     5221 ggtggtgctg gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac
     5281 cgttcgcggc accaaaattc tgctgattaa cccgaccgac tccgacgcag taggtaatgc
     5341 tgtgaagatg gctaaccagg cgaacatccc ggttatcact cttgaccgcc aggcaacgaa
     5401 aggtgaagtg gtgagccaca ttgcttctga taacgtactg ggtggcaaaa tcgccggtga
     5461 ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgctgg
     5521 tacatccgca gcccgtgaac gtggcgaagg cttccagcag gccgttgctg ctcacaagtt
     5581 taatgttctt gccagccagc agattttgat cgcactaaag gtttgaacgt aatgcagaac
     5641 ctgttgaccg ctcatccgga tgttcaggct gtattcgcgc agaatgatga aatggcgctg
     5701 ggcgcgctgc gcgcactgca aactgccggt aaatcggatg tgatggtcgt cggatttgac
     5761 ggtacaccgg atggcgaaaa agcggtgaat gatggcaaac tagcagcgac tatcgctcag
     5821 ctacccgatc agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct gaaaggcgag
     5881 aaagttcagg ctaagtatcc ggttgatctg aaactggttg ttaagcagta gttttaatca
     5941 ggttgtatga cctgatggtg acataaatac gtcatcgaca gatgaacgtg taatataaag
     6001 aaaagcaggg cacgcgccac cctaacacgg tggcgcattt tatggacatc ccgaatatgc
     6061 aaaacgcagg cagcctcgtt gttcttggca gcattaatgc tgaccacatt cttaatcttc
     6121 aatcttttcc tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt gcatttggcg
     6181 gcaaaggcgc gaatcaggct gtggctgctg ggcgtagcgg tgcgaatatc gcgtttattg
     6241 cctgtacggg tgatgacagc attggtgaga gcgttcgcca gcagctcgcc actgataaca
     6301 ttgatattac tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg ctgatttttg
     6361 ttaatggcga aggtgagaat gtcatcggta ttcatgccgg cgctaatgct gccctttccc
     6421 cggcgctggt ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta ttaatgcagc
     6481 tggaatcacc actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa aataagacta
     6541 tcgttgcgct taacccggct ccggctcgcg aacttcctga cgaactgctg gcgctggtgg
     6601 acattattac gccaaacgaa acggaagcag aaaagctcac cggtattcgt gttgaaaatg
     6661 atgaagatgc agcgaaggcg gcgcaggtac tgcatgaaaa aggtatccgt actgtactga
     6721 ttactttagg aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag cgcgttcctg
     6781 gattccgggt gcaggctgtc gataccattg ctgccggaga tacctttaac ggcgcgttaa
     6841 tcacggcgtt gctggaagaa aaaccattgc cagaggcgat tcggtttgcc catgctgcag
     6901 ctgcgattgc cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt gaagagatcg
     6961 acgcattttt agacaggcag aggtgacgct tggctacaat gaaagatgtt gcccgcctgg
     7021 cgggcgtttc tacctcaaca gtttctcacg ttatcaataa agatcgcttc gtcagtgaag
     7081 cgattaccgc caaagttgaa gcggcgatta aagaactcaa ttacgcgcca tcagccctgg
     7141 cgcgtagcct caaactcaat caaacacata ccattggcat gttgatcact gccagtacca
     7201 atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa cgcggttata
     7261 gtctcgtcct ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat ctggaaacgc
     7321 tgatgcaaaa acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat caaccttcgc
     7381 gtgaaatcat gcaacgttat ccgacagtgc ctactgtgat gatggactgg gctccgttcg
     7441 atggcgacag cgatcttatt caggataact cgttgctggg cggagactta gcaacgcaat
     7501 atctgatcga taaaggtcat acccgtatcg cctgtattac cggtccgctg gataaaactc
     7561 cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt ctcaacattc
     7621 ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac gccatgcgcc
     7681 aactgttatc atatccgctg cgtcctcagg ccgtctttac cggaaatgac gctatggctg
     7741 ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat atcgcggtga
     7801 ttggctatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc actatccacc
     7861 aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg ataacccagc
     7921 cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc ggttcggctt
     7981 agatttacgc tgtcttttga tcaaattatt accatcggtt gttttcagaa gcatgaacat
     8041 tgctgctgaa gcaacagtaa taatgcccat cgtgataaac gtatagtgga attgttcgac
     8101 agtcgttgtg ccttccatcc cttcataaac gcgaaggacg gccgcactta cagcaacgcc
     8161 taaactaatc gacagttgct gcgtgaccgc cagaacactg ttaccgctgc tggcgttgtc
     8221 atcggtcaga tcggcaagtg tgatggtatt catcgcggta aattgcgtcg acatagccat
     8281 ccctaatata aacaacggca agatcagcat ccatatcgcc attgccggtg attgcaaaga
     8341 gaactgagcg atcattagcc caataatcac cgtgatcccc actaacgtat ggcgatagcc
     8401 cagacgacgt aagacttggg taaccatcga ttttgcaata atggaaccta acgctgtcgg
     8461 tgccatcata cagccggcaa taaacgcctg ataaccaaat cctacctgta acatcaatgg
     8521 cataaggaac ggtacactac cggtccccag acgggttgca atattgccta cgataccgat
     8581 cgagaaagtg cgggttttaa ataaatctaa tgaaattaat gggtttggcg tgtgtcgcgc
     8641 atggagaata tagagaagca gtaacccgat gctggtgaca attaccgtca aggcaatcca
     8701 gctggcgaca atcttttccc cgaatagctc tattccgctt gagaagagaa caaggctgag
     8761 gccaaacagc aaaaagccag tgatatcgaa tctgcgttgt gcggtggtga aattgggcat
     8821 atgtttgcgc gcgtaaagaa ggcccgcaat acctatgggg atattgatta aaaatatcca
     8881 gtgccaggtt gccctggtga ccagcacgcc gccaagaacg gggcctaaaa ttggccccac
     8941 cagacccggc atggcgacaa aattcaatac aggaagaagt tcattacgag gataagcacg
     9001 cagtaaggcc agccgagcaa caggcatcat cattgcgccg cctatcccct gaataacccg
     9061 gaagacaacc agctgtggta gcgaattaga aagtgcgcag gccagagaac ccaacgtgaa
     9121 cagactcacg gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta gccatccgct
     9181 taccggaata aacatcgcca ccgtcagcgt ataactgatg atggctgatt gcatcgcgag
     9241 aggagaacga ttaaggctat gagcgattgc gggtaaggcg gtattcagaa tagtggcatc
     9301 aagtgcctgc atgaagaagg ccatcgccgc gatccacggc aaacccgcca tactacgctt
     9361 ctttttatcg ctcattcaat gtcctgttat cgggttatca cttatcaggt gagcgtagca
     9421 gcgcctgaca agttttaaat gccgcgtcgc catcgctttg gataatcgca tcgacaatcg
     9481 cctggtgcag atccagcttt atcactgtgt cgctggtaat tgacgtgaag taagtgtgat
     9541 aaaccgaatg gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc atttcataga
     9601 tatgctcatg ccaggccata tcgacttcga tccagcgttc acggcgaaag ttctttttta
     9661 atgccgccat ttcggccatt aacgtattga gatgcgcctt ctgttccgcg gtgccaaccg
     9721 ttgctgccag taggcaggct tgcggctcca gacagatacg cataaccaga aagtgatcga
     9781 tgacctgatg aaagttctct tctgtcatcc accaggtaag caattcctga tcaagaaaat
     9841 tccagtttga ttgtggcatg acccgggtac caattcgcgg tcgcggtaaa accatccctt
     9901 ttgccgttaa cgttttgacc gcttcgcgta ccgctgtacg actcactcca aattgctcgc
     9961 ccagctcaat ctcaccaggc aaaatggtgc cgggttcata ttcacctttt aagatgcgtt
    10021 gcgccagctt ctcagccaga acatacgaaa ggtttttctg tgcagctaac tgttgtgcgc
    10081 ttaaaggcat tactaatctt cctttttctt tttattcctc cttagtatgc caccaggaag
    10141 tgtgattacg gttgcaaaaa cggcaaattg cttgttttat ggcacattaa cggggctttt
    10201 gct
//
LOCUS       DAJVOY010000148        10137 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVOY010000148 DAJVOY010000000
VERSION     DAJVOY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.148"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..237
                     /locus_tag="OQL90_003209"
     CDS             <1..237
                     /locus_tag="OQL90_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1369882.1"
                     /translation="ETLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDML
                     VYEKTYTAELSSIAGTPIEAGPFDTVVLFKINYN"
     gene            389..1435
                     /locus_tag="OQL90_003210"
     CDS             389..1435
                     /locus_tag="OQL90_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1369883.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTCM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1432..2223)
                     /gene="nei"
                     /locus_tag="OQL90_003211"
     CDS             complement(1432..2223)
                     /gene="nei"
                     /locus_tag="OQL90_003211"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1369884.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2259..2993)
                     /gene="pxpA"
                     /locus_tag="OQL90_003212"
     CDS             complement(2259..2993)
                     /gene="pxpA"
                     /locus_tag="OQL90_003212"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1369885.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2983..3915)
                     /gene="pxpC"
                     /locus_tag="OQL90_003213"
     CDS             complement(2983..3915)
                     /gene="pxpC"
                     /locus_tag="OQL90_003213"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCS1369886.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3909..4565)
                     /gene="pxpB"
                     /locus_tag="OQL90_003214"
     CDS             complement(3909..4565)
                     /gene="pxpB"
                     /locus_tag="OQL90_003214"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1369887.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4588..5331)
                     /gene="ybgI"
                     /locus_tag="OQL90_003215"
     CDS             complement(4588..5331)
                     /gene="ybgI"
                     /locus_tag="OQL90_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1369888.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5602..7083
                     /gene="dtpD"
                     /locus_tag="OQL90_003216"
     CDS             5602..7083
                     /gene="dtpD"
                     /locus_tag="OQL90_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1369889.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7125..8543)
                     /gene="phrB"
                     /locus_tag="OQL90_003217"
     CDS             complement(7125..8543)
                     /gene="phrB"
                     /locus_tag="OQL90_003217"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1369890.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8540..9049)
                     /locus_tag="OQL90_003218"
     CDS             complement(8540..9049)
                     /locus_tag="OQL90_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1369891.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9334..9708)
                     /locus_tag="OQL90_003219"
     CDS             complement(9334..9708)
                     /locus_tag="OQL90_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369892.1"
                     /translation="MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKY
                     ENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFKPAKI
                     AMNKNNLIIRTSDVEIAFKITK"
     gene            complement(9693..>10137)
                     /locus_tag="OQL90_003220"
     CDS             complement(9693..>10137)
                     /locus_tag="OQL90_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1369893.1"
                     /translation="IDKPAPVFNRDLNSDPYYLAVNNCYSYALNRYGNPGSRIFGGGG
                     LQPGELSGKEFSKLTCGSIFEASKNDGAKDLDNGSCPSGYHKAQLFIRPHNFIGMGGD
                     YHWYRQDANGEWSDKQGVGAIRFRGKDPFPPIDYPEKCGTIFLPN"
BASE COUNT         2361 a         2750 c         2437 g         2589 t
ORIGIN      
        1 gaaacgttgg ttgaagaaga taaagcattg ttgattggta atggtctgaa gctacgtttg
       61 ctggatgaaa acgcctcgcc ttataccttc aataaatatg ctgaatacgc cgatttcacc
      121 agcgacatgt tggtctacga aaaaacctat acagctgaac tttcgtccat tgcaggtacc
      181 cccatcgagg ctgggccctt cgatacagtg gtgcttttta aaattaacta taactgattt
      241 gccatgatgc cctttgcagc cggaaagggc atctctttca gaaaattgct gattttcctc
      301 ttttccttga ttactgttat tttttcctgg cccggctttc tttatgctgt atcgatacac
      361 caaagagaaa ataatgaggg agcgaaggat gccagttttg cagtggggaa tgttatgtgt
      421 gctatcactt ctcctttcta ttggtttcct cgcggtacat ctcccggcgg cgctattact
      481 tgggccgatg atcgccggga tcatcttcag tatgcgcgga attactctgc aacttccccg
      541 ctccgctttt ctcgccgcgc aggccattct tggctgcatg attgcgcaaa acctcactgg
      601 ttcgattctc accacgctgg ccgccaactg gccgatcgtg cttgctattt tactggtgac
      661 gctgctttcc agcgccatcg tgggttggtt attggtgcgc tatagctcac tgcctggaaa
      721 taccggtgcc tggggttctt ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga
      781 ttacggcgca gatattcgcc tggtggcgtt tatgcaatat ctgcgagtgc tgttcgtcgc
      841 gggggccgct gttctggtca cctgcatgat gctgggtgat aacgctgaag cggttaacca
      901 gcaaatcgtc tggttcccgc cagtgagcat taatctcctg cttacgattt tgctcgcagt
      961 cgttgccggt acggcgggat gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat
     1021 gctggcgggc gcagtgctcc agtctggtca gctcatcacc atcgaactac cggaatggct
     1081 gctggcgatg gcgtatatgg caattggctg gcggattggt cttggtttcg ataaacaaat
     1141 cttactgcgg gcactacgcc cactaccgca aatcctgctg tcgatttttg ctctgctggc
     1201 tatttgtgca ggtatggcgt ggggactgac ccggtttatg catattgatt ttatgactgc
     1261 ctacctcgcc accagtcccg acgggcttga taccgtggcg gtcatcgccg cagggagcaa
     1321 tgccgatatg gcgcttatta tggcgatgca aaccctgcgc ctgttcagta ttttgctgac
     1381 ggggccagcc attgcgcggt ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg
     1441 gcagcctgga caccagtaaa acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc
     1501 acagcgttcg cacagttcgc catcacgatg aaaaacctta aagcgaaaca gcgccccatg
     1561 atgcttattc tcatccacct gcccccgcgt agcgtaagaa aaccgaggaa tctccagtaa
     1621 cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct ttcgctttat gatttccagt
     1681 caacccaacc tgccagagga tctccacccg caaataattg ccaagcccag ccagaaacgc
     1741 ctgatcgagc agtaatcctg caaactgacg gttacgaaag cgcggtgaca ataaccgttc
     1801 ctttaccacc tccggcgtca gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa
     1861 cggatgcgtg gtcagttgtt ccggcctcaa catctcaata tccgaggcgc tataaagcag
     1921 aatggttttg tcagccgttt gcagttttac ccgcaatact cgcgtggtct gcggctcttc
     1981 gccggtatca accacgcgcc agacgccgta aagctgatta tggctgtaga gcgttaagtc
     2041 gttggaaaaa tgagttaaca acgccttacc acgcgttccc acatgggtaa cgtgttgacc
     2101 gataagttgt gattgataag tttttaactg cgggaaggca aaccagacat cagttagtgg
     2161 tttgcctttg atcgccgcct ccaggttatc cgctgcacgg cggatctccg ggccttcagg
     2221 catggtgata tccttttttc attctttatt aatcggggtt atgctgcgac aacaatctcc
     2281 ttttcggcaa atgtagagcg tagtcggcgg gcgaacgcca gtgcgtgctc gccgtcgcca
     2341 tgcaggcaga ctgtttgcgc cgtgaccgtt gcccattcgc cggtgatact tttgactctg
     2401 ccgtgttgca ccatctctag cgtttgcgcc agcgcctgtt cttcgttttc aatcaacgcg
     2461 cctggctgac ttcgcggcac cagcgagccg tcagcctgat aaccgcgatc ggcaaacact
     2521 tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa tcagctcgct tcctgccagc
     2581 ccaacgagaa ccaatgctgg atcgcaagcg tataccgctc tggcgatggc gtctgccagt
     2641 tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg gtttgacatg acgcattacg
     2701 ccgccttgcg cacgggcaat cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa
     2761 acggtttctg gcggcagctg catggcgctg cgaccaaaat tttccctgtc gggaaaactc
     2821 gggtgagcgc caatcgcgac accatttttt attgcttcac gcacgcaagc ctgcatggtt
     2881 tgggcatcgc ctgcatgaaa tccacaagca atattggcag aggaaaccag cgttaatagc
     2941 tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca ggtcaatttt cattgtgtag
     3001 ccgccacgct aattgttcga aataacgttg ttgatcttgc cgcgctttca gtgcctcttc
     3061 cagtgaacac tggacaaaat gaatcggctg accgagcgga atttgcgcca gatggtacat
     3121 atcagcctca atgatacagg caatacgcgg gtaaccaccg gtggtctgtg cgtcgttcat
     3181 caacacaatc ggctgcccgt tatgcggcac ctgcaccaca cccggtaaca aaccgtgaga
     3241 taacagttcg cgatcggtgg tgcgttttaa aatttgcccc tgtaagcgat agcccatgcg
     3301 gttactttgc gggctaagct gccagggcga acgccagaat gcatcctgcg aggcgcgatc
     3361 gaactcatga tattccggcc ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt
     3421 aacgccctgc gcttccataa aatcacgctt cgatttgcca atcgggagtc ggtcaccatc
     3481 cttcagtaaa cggccttcca gcccgccaat ccccacgttg agatcggtgc tgcatgaccc
     3541 cattaccggc ggaacatcaa taccacccgc gaccgccaga taactgcgca tgccgtgctg
     3601 cgggcgttta agcgttaaac gctggcctgc tttcatcggc aagcgccagc cggtccagac
     3661 ggcgttatca tccagccgcg cttcgcaacc ggcacccgtc agagcaaacc acccatcagt
     3721 ttcgaactca accgttaact gaccgagcgt gatctccagc gcgggggcat tggcgtcatt
     3781 acccaccagc aggttagcaa tgcgtaacgc aggcatatcc agtgcgccgc agtggctgat
     3841 acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca gtggtataca tgcccgcacg
     3901 aataatcttc agcatactcc ctccttctgc ggtacaaagc gcacgctgtc tcccggacgt
     3961 aataagatgg gttcgtcacg cgccggatca aacaggctga gtgaggtatg accaatcaac
     4021 tgccagccac ccggcgttgc cagcggataa acaccagtct gcggcccgcc gatcccgaca
     4081 gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc
     4141 aaactcccga gatacgggaa gcccggttga aaacctaaaa accagaccac gtattccacg
     4201 gaggagtgca attcaacaac ctgtttttcg ctcaacccgc aatgcgccgc gaccaccgcc
     4261 aaatccggtc cgcctgcacc accgtaaacc accggaattt caataaagcg agactcaggt
     4321 tccagggcct cgctctcctc ccaccagcgt tgcaaaagct ctatggcatc cagcgccagc
     4381 gactcaggat tacgcaaaat caccgtgata ttgttcatgc cgggaatggc ttcaaccaca
     4441 ttcggcatat ccaccagacg ctgcgccagt cgccagatcc gtttctggct agccagcgtc
     4501 accggcggtt ccagttccag cactaccgcc gtttcaccta tcagataaca acgcgctcgt
     4561 tgcactttcg atccctctga ttattcgtta tgcaggatta ggaatatcaa taaaggtcac
     4621 atcaagatcg gtattttcat tcagccactc gctcaatgcg cgaataccac cacgttcagt
     4681 ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg cgggctgaat gaatggtctg
     4741 ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa
     4801 actttgcccg ccgcccgtgc accaggcgac gcgctggacc acctcaggtc cggtatcgcc
     4861 acaccataac ggcttacgtc ccagacgcgc ttcaatccag gaggctagtt ccagtcccgg
     4921 cacaggcatg gtcagctctc cccacggcac caacggctca atttcgccca tgaccgtgat
     4981 ccccagtaac gccgccagct gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa
     5041 atgccagcca tacaggttga tatcattcgc cagcaacgtt tttaaacggt tacgcttcat
     5101 tccgcgaatg accggagact ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc
     5161 cgcgcccaga cgcactgcct catcgagcaa agcctggctg gcggtgacac cggtaacaat
     5221 tttttgcacc gtctctttgc cttccacctg caaaccattc ggcgcgtaat cactaatcgc
     5281 cgcgctgttc agtttttcgt taatcagttg ttccagttcg gtgtttttca tcttcgcgcc
     5341 ttccgtattc tgatagctac accgttaaca tagcgttacc tcgcctgttc gtcgatagcc
     5401 aatcttttga catattaaga aacaaacccg acgccaaaag acgcaaattg tttatatcat
     5461 cacgcttcag ctaatgtata tttatgcaat cagacagcat attgcataaa taatcagatc
     5521 tgtatcaaag ttttaacttt aaggaaacac aagcacgccg cgcaactatt acgtgacgtt
     5581 gccgtcaatt gtggaattat tatgaataaa cacgcatcac agccgcgcgc tatttactat
     5641 gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg gcatgcgtgc cctgctgatt
     5701 ctctatctca ccaatcaact aaaatacaac gatactcacg cctacgagtt atttagcgcc
     5761 tactgttcgc tggtgtatgt cacgccaatc ctcggtggct ttttggcgga taaagttctc
     5821 ggcaatcgca tggcggtgat gctgggggcg ttattgatgg cgatcggtca tgtggtgttg
     5881 ggtgccagtg agatccatcc gtcattcctc tatctgtccc tggcgataat tgtttgtggc
     5941 tatggtctgt ttaaatccaa tgtcagttgc ctgctcggcg agctgtatga gccaaccgat
     6001 ccgcgtcgtg atggcggttt ctcgctgatg tatgcagcgg gtaacgtggg gtctattatc
     6061 gcacctattg cctgtggcta cgcccaggaa gagtacagct gggcgatggg ctttggcctg
     6121 gcggcggtgg gtatgatcgc gggtctggtc attttcttat gtggcaatcg tcatttcaca
     6181 catacccgcg gcgttaataa aaaagtactg cgtgcgacaa actttctcct gcctaactgg
     6241 ggatggctgc tggttctgct ggtggcaacg cctgcgctga ttaccgtatt gttctggaaa
     6301 gagtggtcgg tatacgcctt aattgtcgcg actattattg gcctgggtgt actggcaaaa
     6361 atttatcgca aagcagaaaa ccagaaacag cgtaaggagc tggggctgat tgtgacgctc
     6421 accttcttca gtatgttgtt ctgggccttc gcacaacagg gcggcagctc gattagcctt
     6481 tatatcgacc gcttcgttaa ccgcgatatg tttggttata ccgttccgac tgctatgttc
     6541 cagtcgatta atgccttcgc agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa
     6601 gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa aatttgctct tggtctcggc
     6661 ctgatgagcg ccgggttctg cattctgacc ttaagcgccc gctggtccgc aatgtatggt
     6721 cactcttctc tgccactgat ggtattaggc ctggcggtga tgggctttgc ggaactgttt
     6781 atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa tccccggtgt gaccggcgta
     6841 ttaaccggca tttatatgct gctttccggc gcgattgcga actatctcgc gggcgtgatt
     6901 gccgatcaga catcgcaggc ttcgtttgat gcttccgggg cgatcaacta ctccatcaat
     6961 gcatatattg aagtatttga tcaaattacc tggggcgcac tggcgtgtgt aggactggta
     7021 ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc gcgcgctggc gctggagtca
     7081 taataggcca gcaccatacc tgataaaacg ctcgctgcgt tttatcaggc tcctttcctc
     7141 gcttcttcgt acgctgccag cgttcgcagc cttgcctctt tgtgatcaac tatcggttgc
     7201 ggataatcca gcatcacacc tgctttctgc gcccacttcc acggctcatg caccgctttc
     7261 cctggtacat cgcgcagctc cggtaaccat cgacgaataa actcgccctc acggtcaaat
     7321 ttctcgccct gggttgtcgg gttgaaaata cgaaaatacg gcgctgcatc ggttccggtt
     7381 gaagcggccc actgccagcc accgttattg gctgccaaat caccatcaat cagctgcgac
     7441 atgaaatatc gctcgccttc gcgccagtcg atcaacaaat ctttcaccag aaaactggct
     7501 gtaatcatcc gtagccggtt atgcatccag ccagtgctgt taagctgacg catggcagca
     7561 tcgacaatcg ggtatcccgt tttgccttcc tgccaggcct gtaaatgtgc gggattgctc
     7621 tgccactgca cacgatccgt ccaggcaata aacggacaat gtttacacaa cgaggggtaa
     7681 tacgtcatca gatgacggta gaactcgcgc cagatcagct cactaagcca gacactaccg
     7741 gccccaccgt ccagcgcctg cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc
     7801 gataacccgc ccgttgccag gctggcggac aaacgactgg tgccttccac tgccggaaaa
     7861 tctcgttgtt gctcatattc tccggcaccg ttctggcaaa actggcgtaa ttgcgcaatc
     7921 gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag actgacgagg ataattcagc
     7981 gtaatggatg gcgagggctc tatcgatccg ctactacgaa cttttggcgc agcgacgcac
     8041 tccggcatcc cttcccgcag ccgtttcagc caggcattct taaaaggcgt aaagactttg
     8101 tacatctcgt gattaccggt catcaccgcg ccaggcggca ggatcacgct gtcatcaaat
     8161 ccttcacaca ccacgttacg cagcgctctt tcaacttcca catcccgcgc ccgctcattc
     8221 acttcatact gatagttata aaacaggtgg gtaacgctgt tttccgcgca cacctgttta
     8281 actatttcga cactggcgac aaagtcatcc acttcacgga acaataaagg aatacctttt
     8341 tccgcaagcg ctatttgtag cccattcagt tgagcattga taagttcagc ctgacgcggc
     8401 gacatgttat gcgtcgccca ctggcgtggt gtagcgatat acaacgccag cacgcgtgca
     8461 gacgaattgc tgcaggcggc agccagtgcg agattatcgt gcaaacgtaa atcctggcga
     8521 aaccagacca gatgggtagt cataaaactc ctgaatggcg caagttaatc acacgcggcc
     8581 aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt
     8641 tgatacgcat cagcggcgca agaagtggtt gctcgccacg gcgataactg tcgataagcg
     8701 cagccagctg ctggcgttct gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca
     8761 tcaaaacatt ggtgtgatcg cgcgggttgg cggggtgaga aagcaaaaca actacgcgtt
     8821 ggcggtactg gttgtaatag tcatccagat tttgccactg gtgaatatca gccacgaacg
     8881 ggccaagttt gcggtactcc ggctgagaat gcgccagaaa gacgagttta tagcggctgt
     8941 gataatcgag aagcgccccg cgcgttaagc catgttcttt cagtcggtgt aactcatgaa
     9001 gggcaaagat acggattagg gtgctgtcat caaatcgttg tagattcatt actgctcctg
     9061 aacggtataa aggagtaagt gtagacgatg gtaaatgaat tttatggggc agagtttttg
     9121 cggggaagta ttttgttggt aaaaatatta tcaacttcat ctgagcgaga attattctac
     9181 tatttcccga gctgcattca ttttttgtat tttttatttt tagaaaataa cagttctcaa
     9241 agcttaatta cctcactatt aattgtattt caaactaaca atcgcactcc attttctctc
     9301 tttaaaaaac ataaacactg ttaaaacaaa tatttatttc gttatcttaa atgctatttc
     9361 tacatcactc gtccttataa tcaaattatt cttattcata gctatttttg caggtttaaa
     9421 atatcggctt tcaccaaagt cagccatgaa aattagacta caagttctcc cagcctttgc
     9481 acatgagaaa aaccacccat caactctatt tattcctgaa cctttaacac cataataaat
     9541 cagaatttct gggtattgtt catccttcac gttttcatat ttttggtatt taattgaaaa
     9601 ccaagagtct atactgccaa catcttcaaa aaatattgga gctgatgttt ccgcatgaac
     9661 atatgcagaa aatgctaaca aaatggcaat acttagtttg gtaaaaatat cgttccacac
     9721 ttttcaggat aatctattgg ggggaaggga tcttttcctc taaatctaat tgctcccact
     9781 ccttgtttgt cagaccattc gccatttgca tcctgacgat accagtgata atcccctccc
     9841 atgcctataa aattgtgagg acgaataaac aattgagctt tatggtaacc agatggacaa
     9901 ctcccattat ctaaatcctt ggcaccatca ttttttgacg cttcaaaaat actaccgcaa
     9961 gtgagtttag aaaactcttt tccagataat tctccaggtt gtaatcctcc gccgccaaaa
    10021 atcctactcc cagggtttcc atacctattt agagcatatg aataacaatt atttacggca
    10081 agataatacg gatctgaatt taggtctcga ttgaatactg gagcaggttt atcaatt
//
LOCUS       DAJVOY010000149        10133 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVOY010000149 DAJVOY010000000
VERSION     DAJVOY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.149"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..611
                     /locus_tag="OQL90_003221"
     CDS             <1..611
                     /locus_tag="OQL90_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1369894.1"
                     /translation="ILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQMIAL
                     RIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSWI
                     VQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTELGSRIICRDKQLIFE
                     EAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            608..1222
                     /locus_tag="OQL90_003222"
     CDS             608..1222
                     /locus_tag="OQL90_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCS1369895.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OQL90_003223"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OQL90_003223"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1369896.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="OQL90_003224"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="OQL90_003224"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1369897.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="OQL90_003225"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="OQL90_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1369898.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="OQL90_003226"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="OQL90_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1369899.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OQL90_003227"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="OQL90_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1369900.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="OQL90_003228"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="OQL90_003228"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1369901.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="OQL90_003229"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="OQL90_003229"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1369902.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="OQL90_003230"
     tRNA            9115..9190
                     /locus_tag="OQL90_003230"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            complement(9205..>10133)
                     /locus_tag="OQL90_003231"
     CDS             complement(9205..>10133)
                     /locus_tag="OQL90_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1369903.1"
                     /translation="KSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEENWKLRA
                     EACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKNK
                     RIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPE
                     ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANT
                     AWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPN
                     HLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
BASE COUNT         2427 a         2496 c         2641 g         2569 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgttct cgcatcatgg tttgcctcaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgtgt attaatcgcc
      121 agatgatcgc tttgcgcata atgcaacaag ttaaggccac gggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcacgt ggtgattact
      301 cctggattgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggcag
      361 ggcgtaaatg ggggcgcacc gagtgtaaat ggcgtctggc agcgaaatac acggtgacgc
      421 aactctcgcg gactgaactt ggcagtcgga taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggccg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggaca ggctaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgt ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggagc aatccgatgc gatccggtat gagacgctgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgtcttgttg aacaggcgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagtg aacggatcag gcagtgaatt tctacgttat tgtcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc aaccacacct ttggctacat cggagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca gtgcgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcaggtc cagttcttct gcatgacccg ccaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tcaccgccgg agtgaccgcc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccaaa
     2281 agcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg gaccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagtgc agaggccata ccaatgccgt tatccgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccggt ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgcgg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgagagctta aagtgctgaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat agttattgat gacctggtgg ataccggtgg tactgctgtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat attccgcaag atacctggat cgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcaggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggct
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggtattg acccacgtga aatcctcgac gtccaggcac gtattgtgat gagcgatgct
     3781 gaacgtaccg acgatgattt atacgatact gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccacccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgacactg gctgcatgcg gctacgttgt acaacattgc cgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa
     4021 gaggccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgtgat gctgactatt gatatgccgt cggtgggctt ttcttcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg tcttaaaggc gctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctgggac gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaattttgcc aatttggtaa aacagttgca tcacaacagg agatagcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgaattctgg ggctggtgga tggagctgga agcgcaggaa
     5041 tcccgtttta cctacagcta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagatactga agtggttgaa cgactggagc acaccctgcg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg ccggatgcat cacatccggc
     5281 aatatctatt aaaactgata cgtcatccca atcgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaatgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattaacca gatcttcacc accgatgccc
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gagtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggag
     5941 tcgccaccaa attccggaaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttttgct gtgcagtatc actctctgct ttattaccgg caaactctgc ttcccaacga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcgcctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact gtcgttatca ctcatataat gcatggcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttgtta tatatttctg cggcctgtac ggatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atatcgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcactt gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg tcgcgatatc gcccacggtg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgcc gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca ttctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggagttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac agtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacctgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcacaa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca tttaaatcaa taagttacac atcattagta cgttccttat ttcttgactg
     9241 ggacaaattt gggaccgatg ggttcagaat cgagtctatt tgccgtgcgt gttcggtaag
     9301 gtgattaggt gcgaggtgag catatcgacg aaccatttcg atagactccc agcctcccat
     9361 ttcctgtaac actgataacg ggactccggc ttgaaccagc caacttgccc aggtgtgtct
     9421 caagtcgtga aatctgaaat catcaatacc tgctcgtctc agcgccgctt tccaggctgt
     9481 gtttgcgtca taccgcatct tcctgactgt tggcgctttc gttccgtctg gtttggtaca
     9541 gctttcctta tacacaaata cccaacggtg atgattcccg atttgttttt tcaatacgcg
     9601 acatgcagta tcattcagcg caacgccaat tgcgcggttt gatttactct cttccgggtt
     9661 tatccatgcc acccggcgct gcatgtctat ttgttgccat tcaaggttga tgatgttcga
     9721 gcgtcttaag cctgttgcaa gtgcaaattc aacaacagac tttaatggct ccggacattc
     9781 atcaatcagc ctttgtgctt catgaggctc cagccagcgg atccgtttat tctttggttg
     9841 aggcacttta ataattggtg ccttatccag cattttccat tcacgctctg cggctcttag
     9901 cagggccttt ataaatgaaa gatgcgtagc cttcgttgca acggacgctg gttttggcgt
     9961 gtattctgga acaggtttcc ctttttttct gcatgcttct gccctgagtt tccagttttc
    10021 ctcatgacgc cggttcgtca ttttctgcat tgctgaataa atttttgatt cagtaatgtc
    10081 tcttagttgc attcctgcga aatgttgaag ccagaatccg atccggcttt tgt
//
LOCUS       DAJVOY010000150        10109 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVOY010000150 DAJVOY010000000
VERSION     DAJVOY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.150"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            205..357
                     /gene="hokA"
                     /locus_tag="OQL90_003232"
     CDS             205..357
                     /gene="hokA"
                     /locus_tag="OQL90_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1369904.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(545..757)
                     /gene="cspA"
                     /locus_tag="OQL90_003233"
     CDS             complement(545..757)
                     /gene="cspA"
                     /locus_tag="OQL90_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1369905.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1038..1328)
                     /locus_tag="OQL90_003234"
     CDS             complement(1038..1328)
                     /locus_tag="OQL90_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCS1369906.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1761..2471
                     /locus_tag="OQL90_003235"
     CDS             1761..2471
                     /locus_tag="OQL90_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1369907.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="OQL90_003236"
     CDS             complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="OQL90_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1369908.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3599..4258)
                     /locus_tag="OQL90_003237"
     CDS             complement(3599..4258)
                     /locus_tag="OQL90_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1369909.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4411..6744
                     /gene="bisC"
                     /locus_tag="OQL90_003238"
     CDS             4411..6744
                     /gene="bisC"
                     /locus_tag="OQL90_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1369910.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6713..7153)
                     /locus_tag="OQL90_003239"
     CDS             complement(6713..7153)
                     /locus_tag="OQL90_003239"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1369911.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7150..7713)
                     /gene="tag"
                     /locus_tag="OQL90_003240"
     CDS             complement(7150..7713)
                     /gene="tag"
                     /locus_tag="OQL90_003240"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1369912.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7871..8569
                     /locus_tag="OQL90_003241"
     CDS             7871..8569
                     /locus_tag="OQL90_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1369913.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8797..9999
                     /locus_tag="OQL90_003242"
     CDS             8797..9999
                     /locus_tag="OQL90_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1369914.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2423 a         2435 c         2591 g         2660 t
ORIGIN      
        1 ttttttagga ttatatcaag gagaagaaac aaacttatta agctagaata gctacgggtg
       61 cttgagactg tttgtctcag gtattcaccg aaaggcagac agagaaaagc cccacctgac
      121 tataaatcaa agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt
      181 ggaaatcaac acagggggct gggaatgccg caaaaatacg gattactttc attaatagtg
      241 atttgtttca cgcttttatt tttcacctgg atggtaagag attcactgtg tgaattgcat
      301 attaaacagg ggcgttatga gctggcggca tttttagcct gcaatttaaa agagtaagag
      361 tcttcggcgg gaaattattc ccgcctttct tacggcgttg cgcattctca ttgcacccac
      421 atttattctt cacaaaaata ataatagatt ttactacgcg atcgattatt tatttcctga
      481 aaaaaaataa aaaaaatccc cgccaaatgg cagggatctt agattctgtg cttttaagca
      541 gagattacag gctggttacg ttaccagctg ccgggccttt agcgccgctt tcgatggtga
      601 aggacacttt ctgaccttcg tccagagatt tgtaaccatc gttctggata gcagagaagt
      661 gtacgaacac atctttagag ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt
      721 tgaaccattt tacgatacca gtcattttac cggacatagt gtattacctt taataattaa
      781 gtgtgccttt cggcgatatg gcgtgcttta cagattttga agcgttaaag gaatgtgcac
      841 tacgaggggt atcaacgata actcttgaag ggacttgcct tactacactg ctttaatggt
      901 ctgtacgtca aaccgttgat gtgcattaag ccacgcattg gcgggtgatg caacaattat
      961 ttttcatatt tatgattaat cggttggaaa acggtcctgt catcaggacc gtaaacagca
     1021 ataaagtgga taaaagtcta ttccatcaac tgcttactta atgccgggtt ggcttgaatc
     1081 aaacgcatca attttagttc ggcacttgaa ggcttcacgc gtctggattc ccattccttt
     1141 accatggcga ctgatatgcc caaaacccgg gcgaaatcat cgatttttaa ccctgtccct
     1201 tttcgtaact gctcaatttc ggtacaggac gttgttctgt gcgtcagggt aattttctgc
     1261 gtttcatctt taaaaacaat ctgttccagg ctgctcaaca gctcatgcat tggatcttta
     1321 tattccattg aaaactcctc aaatcacact gcgggatcgt gaattgcatc gaagctcatt
     1381 aagaatagtc gggaaaagca gacacagcgg ggtgaagtca gggcatgctc tggctatgat
     1441 tcgattcagc cagtcattta gcgtgactta tagccaccta acgatctgat aaatgccgat
     1501 atatctctta ccactcctgt tttgtaaaga tttcgaaaat ctaaagttct gcgaaggcgt
     1561 aaatctcggg ctacaaaaaa gcaacggcaa tatcattttt taacaatgaa acaacaaatt
     1621 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1681 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1741 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1801 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1861 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1921 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1981 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     2041 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2101 tgaacggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2161 ctgcgcactc tgcgctaaaa cagaccgatg atttgaaacc ggtctttgat caggcattca
     2221 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggtt gcacaaactt
     2281 tcacgcaaca actggtgatg gttggagact acattgctca acaaggtact caggtgagct
     2341 ttgtagcaaa tggtatccag ttcccgactt cgcagcaggc aagtgaatat aacaaactga
     2401 ttgcgccgtt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2461 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2521 ttagtccgcg acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2581 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2641 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2701 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2761 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg agggtttcat
     2821 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2881 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2941 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     3001 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3061 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3121 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3181 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3241 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3301 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3361 ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg
     3421 aacggtgaaa tgctcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3481 aacggacggc ttcatgcttc tctccattca ctgataaggc gctcacctta gcctgaaata
     3541 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3601 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3661 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3721 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3781 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3841 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc agggtcgcgc
     3901 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3961 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     4021 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4081 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagtccagac
     4141 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4201 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4261 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4321 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4381 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4441 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4501 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4561 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4621 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc acgtgagttg ggatgaggcg
     4681 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4741 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4801 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4861 gcggcacagg cgatcatgcc gtatgtcgtg ggtggcagcg aagtttatca acagcaaacc
     4921 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4981 aatacgctga aaattgcgtg gaatgcatct gatgagcagg ggctttctta cttttccgca
     5041 ctgcgtgaca gcgggaaaaa gctgatctgt attgatccaa tgcgatcgga aaccgtcgat
     5101 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5161 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt
     5221 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcc
     5281 aaaaccgccg aatgggcggc agggatttgt ggggttcacg cagcgaaaat tcgcgagttg
     5341 gcggctcttt tccaccaaaa cacgaccatg ctgatggctg gctggggaat gcagcgccaa
     5401 cagcttggtg agcaaaaaca ctggatgatc gtcacgctgg cggcaatgtt ggggcaaatc
     5461 ggcacacccg gcggcggttt tggtctttct tatcattttg ccaatggtgg taatcccacg
     5521 cggtgttctg cggtgctctc ttccatgcag ggtagcttgc cgggtggcag cgatgcggta
     5581 gataaaatcc ctgttgcccg tattgttgaa gcactggaaa accctggctg cgcataccaa
     5641 cacaatggca tgaatcgcct cttcccggat attcgcttta tctggtgggc gggtggcgcc
     5701 aacttcaccc atcatcagga cactaatcgc ctgatacgag cttggcaaaa accggagctg
     5761 gtagttatct ccgaatgctt ctggacggcg gcggcaaaac acgcggatat cgttctgcct
     5821 gcgactacct cttttgagcg taatgatctc accatgaccg gcgattacag taatcagcat
     5881 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5941 tttgccgagt taagtgaacg ctgggagaag ggcggttatg cacgttttac ggaaggaaaa
     6001 agtgagttgc aatggctgga aacgttttat aacgttgccc gacagcgcgg ggcaagccag
     6061 caggttgaat tgccgccatt tgctgagttc tggcaagcca atcagttaat tgagatgccg
     6121 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgcaggcg
     6181 catccattaa aaaccgccag cggtaagatt gaaatcttct cgcagcgtat tgccgattac
     6241 gcttatccgg attgcccggg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6301 gccgaaccgg aacagttaca ggtactttct gctcatccgg cgcatcgtct gcacagccag
     6361 ctgaattaca gttctctgcg cgaattgtat gcggtggcaa atcgtgagcc tgtcaccatt
     6421 catcctgacg atgccctggc gcgcggcatt caagatggcg atactgttcg gctgtggaac
     6481 gcacgcgggc aaattcttgc cggagtggtc attagcgagg gaattaaacc tggcgtgatt
     6541 tgtattcacg aaggggcatg gccggatttg gatttaaccg ctgacggtat ttgtaaaaac
     6601 ggcgcagtca acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6661 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6721 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6781 aatcgacaat gtgaaaaccc tgcgcctggt aaaaatttat cgccggttga tttttttgat
     6841 aaacctccag catcaggtgg ggatggcgct gctgcacata ttgcatcagc gccttaccaa
     6901 taccgcgcct gacggccttc ggtgcgacaa acatcgctgc cagaaatcgg ccttccataa
     6961 tgctgacaaa accgagaagc ttactgtctt cttcccagac ccagttttgc gcattggcaa
     7021 gatacgcatc ccgcaccagc ggaatgcaat cacgccagta attcgctttt ataaagggat
     7081 gtccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7141 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7201 cagcccacat gcctgcataa aggagtaaca gattgtggta ccgacaaact taaaaccacg
     7261 ttttttcagt gctttagata gggcgtcgga ggcgggcgta gatgtgggga tttcgctcaa
     7321 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcggcaaa
     7381 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7441 tttcccgcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7501 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7561 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7621 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7681 cgggtcctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7741 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7801 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7861 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7921 cgtggtaatc agcgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7981 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     8041 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8101 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8161 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8221 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8281 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8341 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8401 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8461 tatgttgctc aatcaaaata ttgccgccta tgccgcgcta actcaggcag aaaataccac
     8521 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8581 caattgaaac cattgaataa caatagttgt ggtgatagtg ggtgctaact tacaaataat
     8641 aaatttggtg aataattgtc gcgtcattca ttcctgaact aaggcatttc attccgttct
     8701 gatggcattt catgccgttt ttccccaggc ataaaatgca cttcgttatg gttgtcggca
     8761 gagatttttc ctttttatta ctgcaggaat actgccatga caccttcaaa ttatcagcgt
     8821 acccgctggc tgacactcat cggtactatc attacccagt ttgcgctggg gtcggtttat
     8881 acctggagcc tgtttaatgg cgcgctttct gccaagctgg atgcgccggt aagccaggtc
     8941 gctttctctt tcggcttgtt aagtctgggg ctggcaattt catcttctgt tgcggggaaa
     9001 ttacaggaac gttttggcgt taaacgcgtc actatggctt ccggcatttt gctaggatta
     9061 ggcttcttcc tgacagcgca ttctgacaac ctgatgatgc tgtggttaag cgccggtgtg
     9121 ctggtcggtc tggcggatgg cgcgggttac ctgctgacgc tctctaactg cgtgaagtgg
     9181 ttcccggagc gtaaaggtct gatctccgcg ttcgctatcg gttcttatgg tcttggtagc
     9241 ctgggtttca aatttatcga cacgcagctg ctggaaacgg tgggtctgga aaaaacattt
     9301 gtgatttggg gagcgattgc gctggtgatg attgttttcg gcgcaacgtt aatgaaagac
     9361 gcaccaaaac aggaagtgaa aaccagcaat ggtgtggtgg agaaagatta cacgctggca
     9421 gagtcgatgc gtaaaccgca gtactggatg ttagcggtaa tgttcctgac tgcctgcatg
     9481 agcggcctgt acgtgattgg ggtagcgaaa gatatcgccc aaagtctggc acaccttgat
     9541 gtggtttccg cagccaatgc agtaacggtt atttccatcg ctaacctttc aggtcgtctg
     9601 gtgctgggca ttctgtctga caaaattgcc cgtatccgtg ttatcaccat tggtcaggtg
     9661 atttcgctgg tgggtatggc ggccctgctg tttgcaccat taaatgcagt gacgttcttt
     9721 gcagcgattg cctgcgtggc atttaacttt ggcggcacta ttaccgtctt tccatcactg
     9781 gtcagtgagt tcttcggcct caataacctg gcgaaaaact acggtgtgat ttacctcggt
     9841 ttcggtatcg gtagcatttt tggttcgatt atcgcctcac tgtttggcgg tttctatgtg
     9901 accttctacg tgattttcgc cctgctgatt ctgtcattgg cgctttctac gacgattcgt
     9961 cagccagagc agaaaatgtt gcgtgaggcg catatttaga aatatgttgc tactgaaata
    10021 aatacgagga gggagacttc aaagtctccc ttttttatgg acgcatatta aaagaacaat
    10081 aatattcata ccacaaatat attttattt
//
LOCUS       DAJVOY010000151         9984 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVOY010000151 DAJVOY010000000
VERSION     DAJVOY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.151"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(262..1626)
                     /gene="tdcG"
                     /locus_tag="OQL90_003243"
     CDS             complement(262..1626)
                     /gene="tdcG"
                     /locus_tag="OQL90_003243"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1369915.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1698..2087)
                     /locus_tag="OQL90_003244"
     CDS             complement(1698..2087)
                     /locus_tag="OQL90_003244"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1369916.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2101..4395)
                     /gene="tdcE"
                     /locus_tag="OQL90_003245"
     CDS             complement(2101..4395)
                     /gene="tdcE"
                     /locus_tag="OQL90_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1369917.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4429..5637)
                     /gene="tdcD"
                     /locus_tag="OQL90_003246"
     CDS             complement(4429..5637)
                     /gene="tdcD"
                     /locus_tag="OQL90_003246"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1369918.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5663..6994)
                     /gene="tdcC"
                     /locus_tag="OQL90_003247"
     CDS             complement(5663..6994)
                     /gene="tdcC"
                     /locus_tag="OQL90_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1369919.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(7016..8005)
                     /gene="tdcB"
                     /locus_tag="OQL90_003248"
     CDS             complement(7016..8005)
                     /gene="tdcB"
                     /locus_tag="OQL90_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1369920.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(8104..9042)
                     /gene="tdcA"
                     /locus_tag="OQL90_003249"
     CDS             complement(8104..9042)
                     /gene="tdcA"
                     /locus_tag="OQL90_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1369921.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            9231..9575
                     /gene="tdcR"
                     /locus_tag="OQL90_003250"
     CDS             9231..9575
                     /gene="tdcR"
                     /locus_tag="OQL90_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1369922.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         2690 a         2512 c         2271 g         2511 t
ORIGIN      
        1 aatattttgt agggcttatt atttttttcc gagccgcatc aaggcgatat gcggtctacg
       61 tttttgcagg cgagttatta gaataaagaa atgatacgcg cgaggtcaga gaaaatgttc
      121 acaatcaaga ctttgaaata acggagatgg ataagaattt tctactaaat taatcgcaag
      181 agaatattca ttctctattt gcggcagatc acaaaaaaag gtgcacattt gtgcacccaa
      241 ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc ctccgcgtga
      301 tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct cgatcacttt
      361 atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc
      421 tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg
      481 atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc atacctgcgc
      541 cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg ccacgccaat
      601 ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat acagcgcgcc
      661 aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg gacgacggaa
      721 cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag tcggtgccgt
      781 taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca tgttgatcca
      841 gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct gacgacgcag
      901 cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag tgttcatccc
      961 gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt caatttccgc
     1021 tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca ggccgttgta
     1081 atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg acgtttcgac
     1141 atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga cagagtaata
     1201 ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc cgttctcatg
     1261 gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat caacaatatg
     1321 cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa atgcagggat
     1381 ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa tggcgacatc
     1441 cgtggcatgg cctttgcccg tcagtgacaa cgccccatac agatcgacca caatatggct
     1501 cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc cggcattcat
     1561 tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat cgaatgcact
     1621 aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga ccttgcgacc
     1681 gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc cagcttcaca
     1741 tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc ctgatgctca
     1801 tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc ggtgatgaac
     1861 acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat cgctttgacg
     1921 ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc ggtctgcggg
     1981 caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc ctgaacataa
     2041 gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca tcctccggcg
     2101 tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc
     2161 gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt tttccgggtg
     2221 ctcgatggca tccagcagca tttcccgatt cattatgttg acgttgaggt gttgaccgcc
     2281 ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt tagttttacg
     2341 tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg agatcccatc
     2401 tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg cacctttgcg
     2461 gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc
     2521 cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca gaatcgactg
     2581 ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa agcgttcaac
     2641 caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc
     2701 gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca cgcgggcata
     2761 tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc atgccatagt
     2821 gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg
     2881 catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt ccatgaagtg
     2941 atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt
     3001 cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt agagcagcgt
     3061 tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca ccattgggct
     3121 gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca gatcgtcatt
     3181 ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt tgaaggcaat
     3241 cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg
     3301 cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt ccagccccat
     3361 cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat caaattccgg
     3421 tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca gttcctgtgc
     3481 ctgctgctca ttgagtacgc cagcttcaaa gtcgcgctca atgtagatat cgaggaacga
     3541 tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat ttcactgccg ccagataagc
     3601 gaagtagagc cactgcaccg cttcttgcgc attctgcgcc gggcgagaga tatcaaagcc
     3661 atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg ccagctcctc
     3721 acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact ggagatcggc
     3781 aaattgcagt tcgcgttcac gtaccagata actgatgcca tacagcgcta cgcggcgata
     3841 gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca cgccagattt
     3901 acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat gggttttacg
     3961 cagatcggta aacagatatt caaattcact gtccatttca cggccatagg cgtggaatga
     4021 acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg gcgcatccgt
     4081 ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat gagcggtaat
     4141 tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat
     4201 accttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg cgaggaaaga
     4261 ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat taatttcgtt
     4321 tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat cgctggtatc
     4381 aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt atgcaaattc
     4441 tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt
     4501 agtcggaata acagcgcaaa tgacacgcgc attttcactg gaaacaattc gctcaccaca
     4561 ggagttagag cgattattca tttctgtatc aatctctacg cctaatacag ccaaatgttc
     4621 catgaccaga cgacgaatta agcttgaatt ctctcctatt ccgccggtga atataattcc
     4681 atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc ggtgaacaaa
     4741 ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt ccagaacacg
     4801 taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca ctacgcgttc
     4861 caagtcaccc aagctctggt tggtttggct ggcgacccag gacatcgcac caaaatcgac
     4921 atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca ttgaggtgtc
     4981 aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat gcgccacaac
     5041 caggccggag tcatcttccg ctagattcag caaagaatgt gcgcgctggg aaacatagcg
     5101 gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat atttccacgg
     5161 caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat caaataccgc
     5221 cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta aattggcgta
     5281 attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgacct catcggtaat
     5341 aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa ttaaggccac
     5401 actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg caccttcgta
     5461 gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg cattttctga
     5521 gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat cgagtacgga
     5581 aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt cattcatctc
     5641 ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg atggtcagca
     5701 gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc agagacggtg
     5761 ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc ggtgcgccca
     5821 tggcttcaat aaggtcgagg atgttcgggt tggcgtaggc aacaacccag gtggagccca
     5881 tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt ttgtcgcctt
     5941 tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga tagtgaccga
     6001 agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc agtgtaatcg
     6061 cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa gaaagcactg
     6121 gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac aggcagctaa
     6181 aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg atttgggaac
     6241 atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt ttagaaacca
     6301 cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa atccccagcc
     6361 agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt
     6421 cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc aggctggcga
     6481 taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca aaccagatga
     6541 cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc ggtgcaaatc
     6601 cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca taaatccaca
     6661 gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca gttttaccaa
     6721 aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa agacacagac
     6781 gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac atcagaagaa
     6841 tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg gcgccgattg
     6901 ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac gattgttttg
     6961 tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga attaattaag
     7021 cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg ccggaaataa
     7081 tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat aatgcagcac
     7141 atgccagagc acctgcgcct tcggtgacga ctttattgcg ctgaattaag gcaatcatac
     7201 tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca cgaacgattt
     7261 cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg ccggtagttc
     7321 ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg ttttctgact
     7381 gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca ataccagcaa
     7441 ttaaaccacc accaccaatt ggcacaatca cgttatcgac atcatagaga tcttccataa
     7501 tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa ggtgggataa
     7561 aaatacgacc ttccatttcg acaatttcgc tcactttagc gatagtgtcg ttgaagttat
     7621 caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg gattttggcg
     7681 caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag agcgaaaccc
     7741 cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc gcatcggtca
     7801 gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc tgcatatttt
     7861 caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag cgaggcatgc
     7921 ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca tcaatagcaa
     7981 ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa atatgtagat
     8041 aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa taaaaacaaa
     8101 taactaacca acttctatta atagccttcg tctacaccca ttataagatg aatactcttt
     8161 ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg accataccgc
     8221 ggcatattgt gccacaggta atgtttcttc aactggaata gtaataaatt gatttgaacc
     8281 aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga gaacaagatt
     8341 ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc catttctttg
     8401 taacgtagta agaagctcgc tgtagtaccc catattggtt tgtggcaaca cccactgttc
     8461 gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac tggctaccag
     8521 cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca tttctgcact
     8581 taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga acgaagacag
     8641 ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact tgttgatcat
     8701 ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt ccaccaccgc
     8761 ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg taatggattc
     8821 ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat ttttccgcac
     8881 cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga cggccggttg
     8941 agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga cttcctgaaa
     9001 gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct acgttaatta
     9061 cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc gaccactcac
     9121 aaattaactt tcattacttt ccagggtatc gttattacaa ttaaatatct ttaaatatca
     9181 acaagttaaa acataaaaat cagcataaac cctgattttt atcaaaaaaa atgacgggga
     9241 taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa aaggggctga
     9301 gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta aatttaatga
     9361 gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact gcggcacgtc
     9421 acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat tttaaaattt
     9481 cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc tcttctgaca
     9541 ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca taggcgacga
     9601 atagcatttt gtgttgagga tcacaaaaca aataattgct ggtcgccgcg ataaggtcag
     9661 acgaagtcaa caaggggaat tttcactgtg ctttacatca gcgtaggaca cagaatgttg
     9721 cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgtaa gtgtcgtgcg
     9781 aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca atgaaaggct
     9841 tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgcagtg gttaacaatc
     9901 acaatagaaa tattgattat tggacggtaa aaagaaagtt tgcagaaatt gtctccacca
     9961 atgacgttaa aaaaatttac agta
//
LOCUS       DAJVOY010000152         9932 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVOY010000152 DAJVOY010000000
VERSION     DAJVOY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.152"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            235..432
                     /locus_tag="OQL90_003251"
     CDS             235..432
                     /locus_tag="OQL90_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369923.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(382..1575)
                     /locus_tag="OQL90_003252"
     CDS             complement(382..1575)
                     /locus_tag="OQL90_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1369924.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1711..3435
                     /gene="iucA"
                     /locus_tag="OQL90_003253"
     CDS             1711..3435
                     /gene="iucA"
                     /locus_tag="OQL90_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1369925.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3436..4383
                     /gene="iucB"
                     /locus_tag="OQL90_003254"
     CDS             3436..4383
                     /gene="iucB"
                     /locus_tag="OQL90_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1369926.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4383..6125
                     /gene="iucC"
                     /locus_tag="OQL90_003255"
     CDS             4383..6125
                     /gene="iucC"
                     /locus_tag="OQL90_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1369927.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6122..7459
                     /gene="iucD"
                     /locus_tag="OQL90_003256"
     CDS             6122..7459
                     /gene="iucD"
                     /locus_tag="OQL90_003256"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1369928.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7465..9660
                     /gene="iutA"
                     /locus_tag="OQL90_003257"
     CDS             7465..9660
                     /gene="iutA"
                     /locus_tag="OQL90_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1369929.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWIIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2382 a         2562 c         2641 g         2347 t
ORIGIN      
        1 gaaacctggc gttgggttga aggatataat aaagcaacag aactggctcg ccaatgccct
       61 gatacccatg tattgtcaat ttctgatcgt gaaggcgatt tttatgacct gtttgaacgc
      121 gcagagcaga ctcccggaat aaaagctgac tggcttgtca gaatgaaatt taataacaga
      181 gcaacgctga atattaatgg taaacgggat cataggttgt tgcacgaaag aatcatggag
      241 attacaccgc agcaactggt tgaattcact atacctgatg ggcgggggca gcaagccaga
      301 acactgcctg acatgctttc atcagacgca ggtcctctct catatcctct gatttactcc
      361 agtaaggtga tgatcatatc tttatttgat agtacggtaa tgccagataa atacagacac
      421 aatggtaaat aatatagttg caaataatgc tgccataatc agcgcgattg cgacacctgt
      481 tttcgatacc agcggcacca gcaaagcgca cagtccatag ccaagtgtgt gactggtagc
      541 gagccagccc gcgcctgcgc catcagccag cttatcattc agtagaagtt gataagcggg
      601 tgttgccaga gctgctccaa atgacagcac tgcacagccc gggtaaaaca accatagctg
      661 ttcggagagc atgatagcca gaccaccact catcaacact ccggcactca ggagcagggc
      721 caccggagtc aggcgctgcg gacggactac cccgaactgc gcgataagcg cagctactgc
      781 ggacagaccc aacagccagg ccacctgttg gctaatggcg gtggtatccg tgacgaactg
      841 gcgagtaagg gcaggcgaaa gtccaagctg catcatgctc accgccgcag ccagtaatat
      901 tgcgcaaagc agataaggca gacaatcccg cttcagactg acgctcttgc actccggtgt
      961 gggctgtggt ggtgtgccgg gcaaccgcag cagcatcagc agcgccagca ctggtgctgc
     1021 catcagtagc cccagtggcg ccagaggatg aatggccagc attgctgccg cgcacagcgg
     1081 cccgaatagc ctgccgcaac tcaggccgga gctgatggtt gccagagcgg ccatgcgatt
     1141 cccttctcca gctctctgca atgcccagac ctgacaggct ggcaccattg ccgacacggt
     1201 cagcccgtag gcgatccggg cgatgattaa tattcccagt cccaccgctg ttgttatggc
     1261 gctggtagcc atcagcacgc ttcccagccc tagcagggta aagcttatgc catatcctcc
     1321 cagagcccac aacaccacag gtttactgcc agtacgggaa atttgctttc cccaccacgg
     1381 ggacgatggc agaaacagca ttgagccaag catcagcagg gcagcccata cagacagact
     1441 cagatttgtc tgtatgacca gaacagggag cacaaccagc agaccgttct gtccgatacc
     1501 gagaagcccg gcactgaacg caagtggcca gcaggacagt gttttttggg gcgtatcttc
     1561 gatccctggt gacattataa tgcttcaact ccatgtatta attgtgttta tttgtaaaat
     1621 taatttatct gacaataaca tttattgttg ataatgagaa tcattatcga cataattgtt
     1681 attattttac tgtgtgggag ctgtttgact atgaccctgc cctctgaaaa accagccaca
     1741 gatgtggctg cgcagtgctt cctgaatgca ctgattcgtg aaaccacaga ctggaaactg
     1801 acagaatacc cgccagacga attgctcatc ccgctggatg agcagaaatc gctccatttc
     1861 agagtggctt atttctcccc aacccagcat caccgctttg catttcctgc acgtctggtc
     1921 acggcatcag gcagttatcc tgtcgacttt accactctct cccggcttat tattgataag
     1981 ctacgccatc aactgtttct gcccgttccc ctctgcgaaa ctttccacca gcgcgtgctg
     2041 gaaagccatg tccatacgca acaggcaatt gatgcccgtc atgactgggc cgctctgcgt
     2101 gaaaaagcgt tgaattttgg cgaggctgag caggcgctgc tgacaggaca cgctttccac
     2161 cctgcgccta agtctcatga accgtttaac cggcgggagg ctgaacgcta cctgcctgat
     2221 atggcacccc acttcccact gcggtggttt tcggtggata aaacgcaaat cgccggtgaa
     2281 agtttgcatc tcaaccttca acagcggctg acgcgatttg ccgcagagaa tgcgcctcag
     2341 ttactcaacg aattaagtga caaccaatgg ctgttcccgt tgcacccgtg gcagggggaa
     2401 tatcttttgc agcaggggtg gtgccaggca cttgttgcta aagggctgat taaagactta
     2461 ggtgaggccg gcacgtcgtg gctgccgacc acctcttccc gttccctcta ctgtgccacc
     2521 agccgcgata tgatcaagtt ctccctgagc gtacggctga ccaactccat ccgtactctg
     2581 tccgtgaaag aagtgaagcg tgggatgcgc ctggcacgcc tggctcaaac cgacggctgg
     2641 cagatgctac aggtccgctt cccgactttc cgggtaatgc aggaggatgg ctgggccggg
     2701 ctgctcgatc ttaacggcaa catcatgcag gaaagtctgt ttgccctgcg tgaaaatctg
     2761 ctggtggatc agccgaaaag ccagactaac gtactggtct ccctgactca ggccgcacct
     2821 gatggcggtg attcgctgct ggtttcggcg gtaaaacgcc tgagcgatcg cctcggtatc
     2881 actgtgcaac aggccgccca tgcatgggtc gatgcatact gtcagcaagt gctaaagccg
     2941 ctgtttacgg ctgaagcgga ttacggcctg gtgctgctgg cgcatcagca aaatattctt
     3001 gtccagatgc ttggggatct gccggtcgga tttatttacc gtgactgtca gggcagcgct
     3061 tttatgcctc acgcgacaga ctggctcgat tctattggcg aggcgcaggc ggaaaatatt
     3121 ttcacccatg agcagttgct gcgctatttt ccttattacc tgctggttaa ctccactttt
     3181 gctgtgaccg ctgcgctggg ggctgccggg ctggacagcg aatcgaatct gatggctcgt
     3241 gtacgagcat cgctggctga agtgcgtgat caggtgactc ataaaacctg tctcaactac
     3301 gtgctggaaa gtccgtactg gaacgtaaaa ggtaactttt tctgttatct gaacgatcat
     3361 aacgagaaca ccattgttga cccttcggtg atctatttcg atttcgctaa cccgctgcag
     3421 gctcaggagg tctgaatgtc tgaggcaaac attattcaca gcagatatgg actgcgctgt
     3481 gaaaaactcg acaagcccct gaatcttggc tggggactgg acaatagcgc ggtgttgcac
     3541 tgtcccgggg agctgccgac agggtggctg tgcgatgcgc tggatcagat atttatcgcc
     3601 gcaccacaac tgtcagcagt ggctctcccc tgggccgaat ggcgtgagga gccacaggcg
     3661 ctgacgcttt ttgggcaggt aaaaagcgac attatccatc gtactgcctt ctggcagtta
     3721 ccgttatggt tgagttcacc ggcaaaccgg gcctccggtg aaatggtttt tgatgcagag
     3781 cgtgagattt atttcccgca gcgcccaccc cgtccacagg gtgaggttta ccgccgttac
     3841 gatccacgta ttcgcaggat gctgagtttc cgcattgccg atcccgtttc tgatgcagaa
     3901 cgtttcaccc gctggatgaa tgatccgcgc gttgagtatt tctgggagca aagtggctca
     3961 ctggaggtac agaccgccta tctggagcgc cagctcaccg gtaaacatgc attcccgctg
     4021 attgggtgct tcgacgatcg gccattcagc tatttcgaaa tctactgggc ggcggaagac
     4081 cgcattggtc gtcactattc gtggcagccc tttgaccgtg gcctgcatct gctggttggt
     4141 gaacagcaat ggcgcggggc ccactatgtg caaagctggc tgcgcgggct gacacattac
     4201 ctgctgctgg atgagccccg tacgcagcgc acagtactgg agccacgcac cgataaccag
     4261 cgcctgttcc gccatcttga gcctgcggga taccggacaa ttaaagagtt cgacttccca
     4321 cataagcgct cgcgcatggt gatggcggat cgtcatcact tcttcacgga ggtcggtctg
     4381 taatgaatca caaggactgg gattttgtta accgtcggct ggtggcaaaa atgttgtctg
     4441 agatggagta tgagcaggtt ttccacgcgg aatctcaggg cgatgaccac tactgcatta
     4501 acctgccggg agcacaatgg cgcttcatcg ctgaacgtgg tatctggggc tggctgtgga
     4561 ttgatgccca aacgttgcgc tgcacggacg aaccagtact ggcgcagacg ctgctgatgc
     4621 agctaaagcc ggtgctgtca atgagtgatg ccactgttgc agagcatatg caggatttgt
     4681 atgccacgct actgggcgac ctgcaactgc tgaaggcccg tcgcggactg agtgccagtg
     4741 acctgattaa tcttgatgcc gatcgtctgc aatgcctgct gagcggtcat cctaaattcg
     4801 tttttaataa aggtcgccgt ggctggggca aagaggcgct ggaacgatat gcgccagagt
     4861 ataccaacac cttcagactg cactggctgg cggtaaaacg tgaacatatg atctggcgct
     4921 gcgataacga tctggatatt cagcagttgt tgactgccgc aatggatccg caggagttta
     4981 cacgcttcag tcaggtctgg caggaaaacg ggctggatca caactggcta ccgctgccgg
     5041 tacatccgtg gcagtggcag caaaaaatcg ccacagactt catcgctgat tttgtcgaag
     5101 gcaggatggt gtctctcggc gagtttggcg accagtggct cgcccagcag tcgctgcgta
     5161 ccctgaccaa cgccagccgc cggggagggc tggatatcaa gctgccgctg accatctaca
     5221 acacctcatg ctaccggggg atccctggca gatatatcgc tgccggacca ctggcttcac
     5281 gctggctgca acaggttttt gcgaccgacg ccaccctggt gcaaagcggc gcagtgatcc
     5341 ttggtgaacc ggcggcaggc tatgtgtccc atgaaggcta tgccgcgctt gcccgggctc
     5401 cctatcgcta ccaggaaatg cttggtgtta tctggcggga gaatccgtgc cgctggctga
     5461 aaccggatga aagtcctgtt ctgatggcaa cactgatgga gtgcgacgaa aacaatcagc
     5521 cgctggcagg cgcatatatc gaccgctccg gactggacgc tgaaacctgg cttacgcaat
     5581 tgttccgggt ggttgtggtt ccgctgtatc acctgctttg ccgctacggt gtcgcgctta
     5641 ttgcacacgg acaaaatata acgctcgcca tgaaagaggg ggtaccacag cgtgttctgc
     5701 tgaaagactt ccagggcgat atgcggctgg tgaaagaagc gtttcccgaa atggactctt
     5761 tgcctcagga ggtgcgtgat gccacatccc gtctgagtgc ggactactta atccatgatt
     5821 tgcagacggg tcacttcgtg acagtactgc gttttatttc gccactgatg gttcgtcttg
     5881 gcgtacctga aaggcgattt tatcaactgc tggcagcagt gttgagtgat tacatgaaca
     5941 aacatccaca aatggcagag cgttttgcgc ttttctcact cttcaggcca caaatcattc
     6001 gcgtggtgct gaacccggta aaactgacct ggccggacct ggatggcggc agtcgcatgc
     6061 tgccgaatta ccttgagaat ctgcaaaatc cgctgtggct ggtaactcag gaatatgaat
     6121 catgaaaaaa agtgtcgatt ttattggtgt cgggacaggg ccatttaatc tcagcattgc
     6181 tgcattgtca catcagatcg aagaactgaa ctgtctcttc tttgacgaac atcctcattt
     6241 ttcctggcat ccgggtatgc tggtaccgga ttgtcatatg cagaccgtct ttctgaaaga
     6301 tctggtcagt gcagttgcac ctacaaatcc ctacagcttt gttaactatc tggtgaagca
     6361 caaaaagttc tatcgcttcc ttacaagcag gctacgtaca gtatcccgtg aagagttttc
     6421 tgactatctc cgctgggctg ctgaagatat gaataacctg tatttcagtc ataccgttga
     6481 aaatattgat ttcgacaaaa aaagtcgatt gtttctggtt cagaccagtc ggggagaata
     6541 ttttgcccgc aatatctgcc ttggtacagg aaaacaacct tatttaccac cctgtgtgaa
     6601 gcatgtgaca caatcctgtt tccatgccag tgaaatgaat cttcgtcggc cggacctgag
     6661 tggaaaacgg ataaccgtgg ttggtggagg acagagtggt gcagacctgt tccttaatgc
     6721 attacgcggg gaatggggag aagcggcgga aataaactgg gtctcacggc gtaataattt
     6781 taacgcactg gatgaggctg cttttgctga tgagtatttt acacctgaat atatttcagg
     6841 cttctccgga ctgaaggaag atattcgcca tcagttactg gatgagcaga aaatgacatc
     6901 ggatggcatc actgccgatt ctttactgac catttatcgt gagttgtacc accgttttga
     6961 agttctgaga aaaccaagaa atatccgtct gctacccagt cgctcggtaa taactctgga
     7021 aagtagtggt ccgggctgga ggttattgat ggaacatcat ctggatcagg gcagagagag
     7081 cctggaaagt gatgtggtga ttttcgccac aggttaccgt tctgcattgc cacaaatact
     7141 tccctcactg atgcccttga tcaccatgca cgataagaac acctttaaag tgcgtgatga
     7201 cttcactctg gaatggagtg gcccgaaaga aaataacatc tttgcggtca acgccagcat
     7261 gcaaactcat ggcatcgccg aaccccagct cagcctgatg gcctggcgat ctgcacgtat
     7321 tcttaatcgc gtactggggc gtgatttatt cgatctcagt atgccgcccg cactgattca
     7381 gtggcgcagc ggcagccgga aaaaaccgca gccggaggct gcttctttaa ctcactatac
     7441 agcaaatatt caggaataac gatgatgcgc aaaaagtata tgccccgggc tcttggtccg
     7501 ctgcttcttg tcgtgctgtc accagctgtc gcccagcaaa acgatgataa tgagatcata
     7561 gtgtctgcca gccgcagcaa tcgaactgta gcggagatgg cgcaaaccac ttggattatc
     7621 gaaaatgccg aactggagca gcagattcag ggcggtaaag agctgaaaga cgcactggct
     7681 cagttaatcc ccggccttga tgtcagcagc cagagtcgaa ccaactacgg tatgaacatg
     7741 cgtggccgcc cgctggttgt cctgattgac ggtgtgcgcc tcaactcttc acgttccgac
     7801 agccgacaac tggactctgt cgatcctttt aatatcgacc atattgaagt gatctccggc
     7861 gcgacggccc tgtacggtgg cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa
     7921 ggtcagccgg aaaccatgat ggagtttgag gctggcacaa aaagtggctt taacagcagt
     7981 aaagatcacg atgagcgcat tgccggtgct gtctccggcg gaaatgacca tatctccgga
     8041 cgtctttccg tggcatatca gaaatttggc ggctggtttg acggtaacgg cgatgccacc
     8101 ctgcttgata acacccagac cggcctgcag cactccaatc ggctggacat catgggaacc
     8161 ggtacgctga acatcgatga atcccggcag cttcaactga taacgcagta ctataaaagt
     8221 cagggagacg acaattacgg gcttaatctc gggaaaggct tttccgccat cagcgggagc
     8281 agcacaccat acgtcagtaa ggggctgaat tctgaccgca ttcccggcac tgagcggcat
     8341 ttgatcagcc tgcagtactc tgacagtgat ttcctgggac aggaactggt cggtcaggtt
     8401 tactaccgcg atgagtcgtt gcggtactac ccgttcccga cggtaaatgc gaataaacag
     8461 gcgacggctt tctcctcgtc acagcaggat accgaccagt acggcatgaa actgactctg
     8521 aacagccaac tgatggacgg ctggcaaatc acctgggggc tggatgctga gcatgagcgc
     8581 tttacctcca accagatgtt cttcgatctg gctcaggcaa gtgcttccgg agggctgaac
     8641 aaccataaga tttacaccac cgggcgctat ccgtcatatg acatcaccaa tctggcggcc
     8701 ttcctgcaat ccagctatga cattaatgat atttttaccg ttagcggtgg cgtacgctat
     8761 cagtatactg agaacagggt agatgatttc atcgactaca cgcagcaaca gaagattgct
     8821 gccgggaagg cgatatctgc cgacgccatt cctggtggtt cggtagatta cgataacttt
     8881 ctgttcaatg ctggtctgct gatgcacatc accgaacgtc agcaggcatg gttcaatttt
     8941 tcccaggggg tggcattgcc ggatccgggg aaatattatg gtcgcggcat ctatggtgca
     9001 gcagtgaacg gccatctttc cctgacaaag agcgtgaacg tcagcgacag taagctggaa
     9061 ggcgtgaaag tcgattctta tgaactgggc tggcgcttta ccggtgacaa cctgcggact
     9121 caaatcgcgg catattactc gctttccaat aagagcgtgg aaaggaataa agatctgacc
     9181 atcagtgtga aggacgacag gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg
     9241 atcccggata ctgactggag taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa
     9301 gtgaacggtc aatggcaaaa atatgacgtg aaggaatcaa gtccatcgaa agcgacagct
     9361 tacattaact gggcgccgga accgtggagt ctgcgtgtac agagcaccac ttctttcgac
     9421 gtaagcgatg cagagggtaa cgatattaat ggttacacta ccgtcgattt tatcagtagt
     9481 tggcagcttc cggtgggaac actcagcttc agcgttgaga acctcttcga ccgtgactat
     9541 accactgtct ggggacagcg tgcacctctg tactacagcc cgggttacgg ccctgcttca
     9601 ctgtacgact acaaaggccg gggccgaacc tttggtctga actactcagt gctgttctga
     9661 cggggatgct tttgcaacaa gggtatgctg taaacaacat ggtccttggt ggccatgttg
     9721 tttacagcca gtataatata cagccttaac aagatattcc gtctccggaa tggactgttc
     9781 accactgaat aacattattt ctgttttatg gatctttgac tgaaatttat cgcatatcct
     9841 catttcattg tccgtctgaa aactaacctt acaagtaagc gtcgtctcag caccgtctgg
     9901 cagatcctga aattcctgag aggatgatct gc
//
LOCUS       DAJVOY010000153         9928 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVOY010000153 DAJVOY010000000
VERSION     DAJVOY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.153"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..2097)
                     /gene="fdeC"
                     /locus_tag="OQL90_003258"
                     /pseudo
     CDS             complement(1..2097)
                     /gene="fdeC"
                     /locus_tag="OQL90_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            3213..3314
                     /locus_tag="OQL90_003259"
     CDS             3213..3314
                     /locus_tag="OQL90_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369930.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            3678..3941
                     /locus_tag="OQL90_003260"
     CDS             3678..3941
                     /locus_tag="OQL90_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCS1369931.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            3941..4081
                     /gene="ykgO"
                     /locus_tag="OQL90_003261"
     CDS             3941..4081
                     /gene="ykgO"
                     /locus_tag="OQL90_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCS1369932.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(4116..4343)
                     /locus_tag="OQL90_003262"
                     /pseudo
     CDS             complement(4116..4343)
                     /locus_tag="OQL90_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5166..5708
                     /gene="ecpR"
                     /locus_tag="OQL90_003263"
                     /gene_synonym="matA"
     CDS             5166..5708
                     /gene="ecpR"
                     /locus_tag="OQL90_003263"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCS1369933.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINCRFLRKDIKKDKITDQEMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            5734..6369
                     /gene="ecpA"
                     /locus_tag="OQL90_003264"
                     /gene_synonym="matB"
     CDS             5734..6369
                     /gene="ecpA"
                     /locus_tag="OQL90_003264"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCS1369934.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSNLVVTSLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            6427..7095
                     /gene="ecpB"
                     /locus_tag="OQL90_003265"
                     /gene_synonym="yagY"
     CDS             6427..7095
                     /gene="ecpB"
                     /locus_tag="OQL90_003265"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCS1369935.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            7121..9644
                     /gene="ecpC"
                     /locus_tag="OQL90_003266"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             7121..9644
                     /gene="ecpC"
                     /locus_tag="OQL90_003266"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
     gene            9634..>9928
                     /locus_tag="OQL90_003267"
     CDS             9634..>9928
                     /locus_tag="OQL90_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369936.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNW"
BASE COUNT         2675 a         2449 c         2310 g         2494 t
ORIGIN      
        1 gtttatcagg attcagggtg atggacgaat gtgctgcatc aagcggcccg gcaacaaact
       61 tcagcgtttg ttgtaatgtg cccagctgat actgctcgct gaaagcacgc acacacgcac
      121 aacaacgttt ccggcacggg tcgaagaaac cgtagcgctg taaacgcctg gttttatctc
      181 cgaaattgta ccaatggtga caccattagt gtcttgcgga acaaatcgca agcggctggc
      241 ctctcccgtc accgggttac catcagtatc caccgcagta agcgtcagcg tgtgggattg
      301 ctgaccatca gcaaccacat ctcctgagag ttcattcacg ctcagagtag acttcgacac
      361 atccatcatc gtggcgcgca attctgcagt gacggttttg ctcatgccat caacgctaac
      421 agtaatcgtt gcctcacctg attgcgttcc ggtagtgaag acagactgat acaccccagc
      481 ttcagtttca gtgaactcac ccagtgttgg ctttgcctgt gatttagtgg ccttcaggga
      541 acgagtcaca atatttccag ccggtttgaa agttagttca gtcttgatct gatctttcat
      601 gcccgtgact ggctggccct cggcgtcgcg cagagacagc accagcggtt tttgctcatt
      661 accgttagca agcatttgaa tacggctctg accgtcaagc gttaacgccg tgcgatcggc
      721 gctcatacca gctccggtaa tgaccacctc tgtctgcacg cgttttgagg tattgccttt
      781 gttatcgtag gcaaccgcag aaatcgcata ataattgtct ttgcctggac gataagccgg
      841 gagcgttact tgccactgac taccctgacc ggtaattttg ccaccttcag ccagtaatga
      901 cggcgcttcc cactgcacat ttttcagtcc gtgagttgct ttgctgacca caagcccgag
      961 ggaaagtgtc tgacagccct tgccttcaat acgctcaggc agagcaatac ggatcacttc
     1021 agatttgcgg tactcaagaa cgatgttgtt attacgctca accaggtcat agcggctgcc
     1081 tgctagcatt cgacgctcgc gaatgctgtc tgtatcgagt tgtttctcca gaggttcgcc
     1141 aatccgataa ttaacttcca ggccaaagcg agtgtcattc tcaccactct tgccctgctt
     1201 atgcccggca ctcagggtca gaagaggcac aggcgtgtaa ttcacttcag cggaaatcgc
     1261 atgtggatct ttctggcgtt tatctttgcc aaacagccca acttcatcgc cataatactg
     1321 ctcatacatc aggcttgcgc caagctgcgg tcaggcgggt aaatagccct cagcacgaat
     1381 atcccagcca ttcgccgggc gttcctgata atcctcaata tccggcgatt ttttccagcc
     1441 agaagcccga atataaccat tggcgctcag tttcagataa tcgcgccagt attccgcacc
     1501 aacaccaatg cgggtatgac tacgggataa atcatggtcg ataaaggtgt tcaccccccc
     1561 cattcagtca tttcctgaaa aatgacgcca gccaaaacca atatttgact gagtacgatc
     1621 gtctgtacga tgtattgccc cctgagtgaa caacatattt gtcggcgtat cataaatcgg
     1681 ataaagcatt tccagcgaag aatccttcag cgagaaatct ttatcgacat tcagtttgac
     1741 gcgcgctgta ccatatttcc cgagccactc ctgtatttcc tggttagctt tagcggtggc
     1801 cattccggta ataaaattac gtgtcgcatc gctatctggc tgactgctta aaaatgtccc
     1861 ggcatttgcg gcaaacgacg cgacattttt ctccacgtta ttatcagcag ttaccgtagt
     1921 atttcccatg ctcaaccgtg gctgaaccgc atgctgcgca cgtgccgcca ttactggggt
     1981 aaaggtgaca gcgagtggaa aaagaacctg aacagagata tttgcccacg ccacgcagcg
     2041 ggccagaact gaataacgaa atcgtggttg tttatgacct gttttataac gtgacattga
     2101 gataatttcc tgatgaacga attaacgaac ataggcagac acaaataatg gcgtctgctt
     2161 gtcagaaaaa ccggggattt cccggtttcc ttttccgcag aaaaggaaat acctttatgt
     2221 aagaaagtgc accgccacct gatatatcca gtgtctgtag ccactcatca ggtggttggg
     2281 cgcgggatta tatttttatt tatgggtaaa aatttagctt tttgtgcaag tcattaaata
     2341 gatacctaag ttatatttct tactctgcgg acagtgaata attcattatt cagactgaat
     2401 aataatgacc tgcctttaat tagcaactat tggaaatacg tcgaagtagg tatatacgcc
     2461 aggataaacc gatatattta ttatggatat tcattttcct tcatcggact gtaaattgcg
     2521 gggatatcga acttacacta ctcatcgcaa cactcaaaca gatttatgaa tttcgttcat
     2581 aacccctgac aaatcctgcc gtctaatcga gcttttccct ttacgttatg cttctcatga
     2641 acgagaacac aacttattca tgaattaacc attccggata aactatggaa tcaaattcag
     2701 gcatcgaaag gaactggctg gtttccgtta atagattaaa tatctattac tctcccgaga
     2761 gtaaaactat caaatcaatg agtaaccgat acagcctcac aaattctttc agataattaa
     2821 cccgtcactg cggcacgttc tctcgtaagg cgtgcctgca gtgtaatcca gtatgtatcc
     2881 ggcatagttg acggtttaat agaactcaat tcctctccct ggtcaccaac aacgatatct
     2941 tgtataacac cccaggagat acgacattat gatgagatgc gatatgctcc tcagcaccaa
     3001 tcaacgccag gactgggcca gtcttgagat cagactcatt cccgggactg acttcagtcg
     3061 acatatatca atgacgttgg tgcaataaaa gatagccgat gtaatacatc tattcaaatt
     3121 tgtgatacat gtcaaaaaac tggttgacca aactcgttat ttatataagg gcacttacga
     3181 agtgcgctct tttttaaagc gaggaagtac caatgaaaga gaataaagta caacaaatca
     3241 gtcataaact aattaatatc gttgtttttg tcgcaattgt agaatacgcc tatttatttc
     3301 tccatttcta ttaataacgg caataaactg ttcacttcag tgatatttaa aatatgcatt
     3361 ctctcccttt ttgtaagtaa ttattatatc cgtgggagag gaatacacgc tgtcaggtaa
     3421 tcaatcatac tgcgataaat catcggccag taaagtggag ataacctcca ttgtcgaaaa
     3481 atccatactc tcagcgaaac catcatcaat cactcatcca ggcgtttatg ggagcgtcgc
     3541 caacggctgt taacaatgcc agacttcccc gttgcggaaa ttccacatac cactaatggt
     3601 cacagggatt gggtgttgaa atgatccgga tgagcatgta tctttatggt tatgctataa
     3661 cataacaggt aaaaatgatg aagcccaata tccatcctga gtatcgtact gtggtgttcc
     3721 acgacaccag tgttgatgag tactttaaaa tcggctcgac tatcaaaaca gaccgtgaga
     3781 ttgagctgga tggcgtaacg tatccgtacg tgacaattga tgtctcttct aaatcgcacc
     3841 cgttctatac agggaagctg agaacagtgg catcagaagg aaatgttgca cgattcaccc
     3901 aacgttttgg tcgttttgtt agcacgaaaa aggggtcgtg atgaaagttc ttaactctct
     3961 gcgtaccgca aaagaacgcc atccagactg tcagattgtg aagcgaaaag gacggctata
     4021 tgtgatttgt aaatctaatc cacgttttaa ggccgttcag ggtcgtaaga aaaaacgttg
     4081 attcaaaatt cgacggatta acgatatttg tctgattaat aatcagatcg gattaatgtt
     4141 ggtgtgttta taacaccaac attaattttc ctggggatat attcttcctg ttcatttgag
     4201 gccaactgcc tgacgtttct ctacgaatat tccattatct taatgttgac ttgttgacca
     4261 gcttcgcccc tgtatgctgg catcaactct cttttagact gaacacgcca ctcagtctcc
     4321 tccctttgcg gcgcagcctg cattttcact caaactgtta agatgataaa tgtggtaaat
     4381 ctgttggtac taacataaaa acgtttacgc cacaggaaca gtctgatcca ccggtaaccc
     4441 cgtcgccgac gttcgagtgc cagttagagt aacgcgcaca gataactgca ctcagtgccc
     4501 tgacaaagag gccatcgttc ctgtgacagc tggcagcctt cgtttaactt cacttaatct
     4561 ggctcttgag ggcttaccga acagatgacg tacatacgcc cgttcaattt tccatcactt
     4621 attgtaatga acacctataa ccattttgtg cggcatgtta atccattaaa atttcttact
     4681 gattggcaaa tcatcttcaa tgacagctca ttatagtttt atattctatc ccttaccctt
     4741 aaaacttgtt tttttactag tccatcacac agcgcattaa gactattcct aacacttcag
     4801 ggcaaagttc ctgaccaata taaaatgcaa gtaagaattg aacgttatat tgccaataac
     4861 cttatgaaac caaatgtctt tttcttctta tcaaaaaaac aatattttca gtttttctaa
     4921 atattgactt aaccattgaa ttccttttcc gttcacatat tgacactcat cgaaaaaaaa
     4981 acataaattt aagcccaatc gaaaataatt aaacttaatc tcgtttaacc tttattgata
     5041 tgtactacgt atcttattta cttccggttt actaaggaaa ctgaatgcac ctgtaaaaat
     5101 tacaggtttg gaaagtagtg acatggcaaa gtgattacag tagggactat gaggttaaaa
     5161 accatatgga atgtcaaaac cgttctgata aatacatctg gtctccccat gacgcctact
     5221 tctataaagg actatctgaa ctgattgtgg atatcgacag attaatttat ctatcgttgg
     5281 agaaaattag aaaagatttc gtgtttatca atctcaatac ggattcttta tctgaattta
     5341 taaaccgtga taatgaatgg ttatccgcgg taaaggggaa acaggtcgta ttgattgcgg
     5401 ccagaaagtc agaagcctta gcaaattatt ggtactacaa cagcaatatt aggggcgtgg
     5461 tatacgctgg actgagtcgt gatattagaa aagaactggc ctatgtgatt aattgcaggt
     5521 tcctgagaaa agatattaag aaagataaaa tcacggacca ggaaatggaa attatccgca
     5581 tgacggccca gggaatgcaa cctaaatcga ttgccagaat tgaaaattgt agtgtgaaga
     5641 cagtgtatac ccatcggcgt aatgctgagg ccaagctgta ctcaaaaata tataagttgg
     5701 ttcagtaaac tccaggcaag ttagttttaa aaaatgactc actgggacat cacgtcctca
     5761 attcaactcg ggaagaaatg caatgaaaaa aaggttctgg caatagctct ggtaacggtg
     5821 tttaccggca tgggtgtggc gcaggctgct gacgtaacag ctcaggctgt agcgacctgg
     5881 tcggcaacag ccaaaaaaga caccaccagt aatctggttg tgacgtcact cggtagcttg
     5941 gcgttccagt atgccgaagg cattaaaggt tttaactcac agaaaggtct atttgacgtg
     6001 gctatcgagg gtgactcaac ggctaccgcc tttaaactga cctcacgtct tatcaccaac
     6061 acattaactc agttggatac ctcaggttcc acactgaatg tgggcgtgga ttataacggc
     6121 gcggcagtcg aaaaaactgg cgataccgtg atgatcgata ccgccaacgg cgtactgggc
     6181 ggcaacctta gcccgctggc taacggttac aatgccagca atcgtaccac cgcacaggat
     6241 ggtttcacct tcaccatcat cagcggcacc accaatggta ccaccgcagt aaccgattac
     6301 agcactctac cggaaggcat ctggagcggc gacgttagcg tacagttcga cgcgacctgg
     6361 accagttaat ctctctgatg taccagcagg ggtagccccc ctgctttatt cctggacgga
     6421 attgttatga aaaagcacct tctgcctctc gctctgctgt tttccggaat atctccggcc
     6481 caggcgctgg atgtcggcga tatatcatcg tttatgaaca gtgacagcag cacgctgagc
     6541 aaaacgatca aaaacagtac cgacagtggt cgccttatca atatccgtct cgaacggctc
     6601 tcttcaccgc ttgacgacgg gcaggttatc tcaatggaca agccggatga gttgctactc
     6661 actcccgcca gcttgctgct acccgcccaa gccagcgaag tgatccgctt cttctataag
     6721 ggacccgcag atgaaaaaga gcgctactac cgcattgtct ggtttgatca ggccctcagt
     6781 gatgcgcagc gcgataatgc caaccgcagc gctgtggcca ctgcttccgc ccgcatcggc
     6841 accattctgg tcgtcgcccc tcgtcaggcg aactaccact ttcagtacgc caacggctcc
     6901 ctgacaaata caggaaatgc gacgctgcgg atcctcgcct acggcccctg cctgaaagcc
     6961 gccaacggta aggagtgtaa agagaattac tacctgatgc cgggcaagtc gcgtcgtttt
     7021 acccgcgtgg acactgcgga taacaaagga cgggttgcac tttggcaggg tgataagttc
     7081 attcccgtga aatagatagc tgtgcagatg gataacgaca atgcctttac gacggttctc
     7141 cccaggactg aaagcccagt ttgccttcgg catggtcttt ttgttcgttc agcccgatgc
     7201 cagcgctgct gacataagtg cgcagcaaat aggtggggtg attattccgc aggccttcag
     7261 tcaggcgctt caggacggca tgagcgtccc gctctatatt catctcgccg gtagtcaggg
     7321 tcgccaggac gatcagcgaa tcggcagcgc ttttatctgg ctggatgatg gacagctacg
     7381 catccggaaa atacagctgg aagagagtga agataacgcc agtgtcagcg aacaaactcg
     7441 acagcagctg atggctctgg cgaacgcccc gttcaatgag gcccttacca tcccctgact
     7501 gacaacgcgc agttggatct cagcttgcgc caactgctgc tgcagctggt ggtcaagcgc
     7561 gaagcgctgg gcaccgtact acgctcacgt agcgaagaca tcgggcagtc cagtgttaac
     7621 accctcagca gtaatctgag ctataacttg ggcgtctata acaaccagtt gcgtaacggc
     7681 gggagcaaca catccagcta tctgtcgctg aataacgtta ctgcgctgcg cgaacatcat
     7741 gtggtgctcg acggctcgct gtacgggatc ggtagcggtc aacaggacag tgaattatat
     7801 aaagcgatgt atgaacgcga ttttgccggt caccgatttg ccggtggaat gctcgacacc
     7861 tggaacttgc agtcattagg gccgatgacc gccatttcag cagggaagat ttacggcctt
     7921 tcctggggaa accaggccag ctccaccatc ttcgacagca gccagtcagc cacgccagtg
     7981 atcgcctttt taccggcggc gggcgaagta catctcaccc gtgatgggcg gctactaagc
     8041 gttcagaact tcactatggg caatcatgaa gtggataccc ggggtctacc gtacggtatt
     8101 tacgatgtgg aagttgaggt gatcgttaac ggtcgcgtga tcagcaaacg cactcaacgg
     8161 gtcaataagc tgtttagccg gggcgcggcg tcggtgcacc actggcgtgg caggtatggg
     8221 gcggtagctt tcatatggat cgctggtcgg aaaacgggaa aaagacgcga ccagctaaag
     8281 agagttggct agcaggtgcc tcgacctccg gctcattgag tacgcttagc tgggcggcaa
     8341 cgggatatgg atacgataat caggcggtgg gtgaaacccg tctgacgctg ccgcttgggg
     8401 gggcgatcaa cgttaacctg caaaatatgc tggccagtga cagctcatgg agcagcatcg
     8461 gcagcatcag cgctactcta ccgggaggct ttagttcgct gtgggttaac caggaaaaaa
     8521 cccgtattgg caatcaattg cgacgtagcg atgccgacaa ccgtgcaatc ggcggcacac
     8581 tcaacctgaa ctcactgtgg tcgaagctgg gcacattcag catcagctac aatgatgacc
     8641 gccgttacaa cagccattat tacacggcag attactatca aaatgtctac agcggtacct
     8701 ttggttcgct tggcctgcgg gccggtattc agcgctataa caacggcgac agcaacgcca
     8761 atacagggaa atatatcgct ctcgatctct cgctaccact gggcaactgg tttagcgcag
     8821 ggatgaccca tcaaaacggc tacaccatgg caaacctgtc agcacgcaaa cagtttgatg
     8881 aagggaccat tcgcactgtt ggtgccaatc tgtcacgtgc catctccggc gataccggtg
     8941 atgacaaaac cctcagcggt ggggcgtatg cacagttcga cgctcgttac gccagcggaa
     9001 cgctgaacgt caatagcgcg gcggacggct acgtcaatac caacttgacc gccaatggca
     9061 gcgtcggctg gcagggtaaa aacattgctg ccagcgggcg gactaatggc aacgctgggg
     9121 tgatattcaa caccgggctg gaggacgacg gtcagatcag cgccaaaatc aacgggcgga
     9181 ttttcccgct taacggcaag cgtaactatc tcccgctctc tccctatgga agatatgagg
     9241 tggagttaca gaacagcaaa aactcactcg acagttacga tatcgtcagc ggccgcaaaa
     9301 gtcgtctgac tctctatcca ggcaatgtcg ctgtcattga gccagaggtg aagcagatgg
     9361 ttaccgtctc cggtcgtatc cgtgcggaag acggcacact gctggctaac gcacggatta
     9421 acaaccatat cggccgaacc cgaaccgatg aaaacggcga gtttgtcatg gacgtggata
     9481 agaaataccc cactatcgat tttcgctaca gtggcaataa aacctgcgaa gtggctctgg
     9541 aactcaacca ggcgcgcggt gccgtctggg tcggtgatgt ggtctgcagc ggcctctcat
     9601 cgtgggcggc ggtgacgcag acaggagaag agaatgagag ttaacctact aataacgatg
     9661 ataattttcg cgctaatctg gccagtaact gcgctcagag cggcagtgag caaaacaacc
     9721 tgggcggatg caccggcacg cgagtttgtg tttgtcgaaa acaactcaga cgacaacttt
     9781 ttcgtcactc ctggcggggc gctggatccg cgcctgaccg gtgccaaccg ctggaccggt
     9841 ttaaaataca ctggttcagg aaccatctat cagcaaagcc tcggctacat tgataacggt
     9901 tacaacaccg gcctttatac caactgga
//
LOCUS       DAJVOY010000154         9886 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVOY010000154 DAJVOY010000000
VERSION     DAJVOY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.154"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OQL90_003268"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OQL90_003268"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1369937.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OQL90_003269"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OQL90_003269"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1369938.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3389)
                     /locus_tag="OQL90_003270"
                     /pseudo
     CDS             complement(2698..3389)
                     /locus_tag="OQL90_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3519..5279
                     /locus_tag="OQL90_003271"
     CDS             3519..5279
                     /locus_tag="OQL90_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS1369939.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5685..6542
                     /gene="focA"
                     /locus_tag="OQL90_003272"
     CDS             5685..6542
                     /gene="focA"
                     /locus_tag="OQL90_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1369940.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6597..8879
                     /gene="pflB"
                     /locus_tag="OQL90_003273"
     CDS             6597..8879
                     /gene="pflB"
                     /locus_tag="OQL90_003273"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1369941.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9071..9811
                     /gene="pflA"
                     /locus_tag="OQL90_003274"
     CDS             9071..9811
                     /gene="pflA"
                     /locus_tag="OQL90_003274"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1369942.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2532 a         2487 c         2355 g         2512 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtttc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgtccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgaag atggaataat ccagtatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgagg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgttttccc ccgctttcat atgaatgatt tcactacaac gaatacaggc atagtccgcc
     2821 gactccggca ccactttgta acgctgcgta caatcactgg gtaaaataag taaacccggt
     2881 ttcaccttgt catcttcaaa gaaatagaca ctgatttgcc cgttggtttc gagaatcgcc
     2941 agacgtacct gccccagctg ctccacgcca cgcaatcgta gctccataaa gaactcaaat
     3001 tccgtcatgt tggagttatt gagtttcgac caggccagct cgccatcttc aataataaca
     3061 accggcttac cttccagtag atcttccagt ttttcactgt gcgccatcaa ccacattacc
     3121 aggcggtata acaacgccag agtgataaag acgataagta ccggcaccat cggcacatca
     3181 tcataaaacg ccacatctcc cgccgctgaa cccagcgtga gaatgattaa cacttcaaac
     3241 agcgacatct gtcgcacacc gcgtcttccg gtcattttga ggaacaaaaa aaccagtaca
     3301 aaggtataaa gactacgtag tgcaacttcg ccaaggaaat caaaaggcac tttatcaaat
     3361 gccatacggt ggagatcgaa cgccttcatt tttatgctct tatcgtggtt tattcgctaa
     3421 aagcatagtc aacagttcat cggcatggcg gcggtgatag cgcctttgag ataaaacccg
     3481 tattattgcg cgctttccgt acgactaaag tgattttcat gacgcaaaca tttatccccg
     3541 gcaaagatgc cgctctggaa gattccatcg ctcgcttcca gcaaaaactt tcagacctcg
     3601 gctttcagat tgaagaggcc tcctggctga atcctgtgcc taacgtctgg tctgtacata
     3661 ttcgcgacaa agagtgcgca ctgtgtttta ccaacggtaa aggcgcaacc aaaaaagcgg
     3721 cgctggcttc tgcacttggt gaatatttcg agcgtctctc aaccaactac ttttttgctg
     3781 atttctggct gggcgaaacc atcgccaacg gtccgttcgt gcattatccc aacgaaaaat
     3841 ggttcccact gaccgaaaat gacgatgtgc cagaaggact actcgatgac cgtctgcgcg
     3901 cgttttatga tccggagaat gaactgaccg gcagcatgct gattgaccta caatccggta
     3961 acgaagatcg tggtatttgc ggcctgccgt ttacgcgtca gtccgacaat cagaccgttt
     4021 atattccgat gaatatcatt ggtaacctgt acgtctccaa cggtatgtcc gcaggtaata
     4081 cccgcaacga agcacgcgtt cagggattgt ctgaagtttt cgaacgctac gtgaaaaacc
     4141 gcattattgc tgaaagcatc agcctgccag agatcccggc agacgtgctg gcgcgttacc
     4201 cagcggtggt tgaagcgatc gaaacactgg aagcagaagg tttcccgatc ttcgcatatg
     4261 atggttccct tggcggccag tatccggtga tttgcgtggt actgttcaat cctgctaacg
     4321 gtacctgctt tgcctctttc ggtgcgcatc ctgattttgg cgtagcactg gaacgtaccg
     4381 tgaccgagct gctgcaaggt cgtggcctga aagatttgga tgtgtttact ccgccaacct
     4441 tcgatgatga agaagtcgct gaacatacca acctcgaaac gcactttatc gattccagcg
     4501 gtttaatctc ctgggacctg ttcaagcagg atgccgatta tccgtttgtg gactggaatt
     4561 tctccggcac cacggaagaa gagtttgcta cgctgatggc tatcttcaac aaagaagata
     4621 aagaagttta tattgccgat tacgagcatc tgggcgttta tgcttgccgt attatcgtgc
     4681 ctggcatgtc cgatatttat ccggctgaag atctgtggct cgcgaataac agtatgggca
     4741 gccatttacg tgaaacgatt ctttcgctac caggcagcga gtgggaaaaa gaagattacc
     4801 tgaacctcat cgagcaactg gatgaagaag gttttgatga ctttacccgc gtgcgtgagc
     4861 tgttgggtct ggcgaccggg tcggataacg gttggtacac cctgcgtatc ggtgaattaa
     4921 aagccatgct ggcgctggct ggtggcgatc tggaacaggc tctggtctgg accgaatgga
     4981 cgatggagtt taactcatca gtatttagtc cggaacgcgc caactattat cgctgcctgc
     5041 aaacgttgtt attactggca caggaagaag atcgccagcc gctgcaatat ctgaatgcgt
     5101 ttgttcgcat gtacggcgca gatgccgtgg aagccgccag tgcggcaatg agcggcgaag
     5161 cggcgtttta cggcctgcaa ccggtagata gcgatctgca cgcgtttgct gcacatcagt
     5221 cgttgttgaa ggcctacgaa aagctgcagc gcgccaaagc agcattctgg gcaaaataaa
     5281 atcaaatagc ctacgcaatg taggcttaat gattagtctg agttatatta cggggcgttt
     5341 ttttaatgcc ccgctttaca tatatttgca ttaataaaat aattgtaatt ataaggttaa
     5401 atatcggtaa tttgtattta ataaatacga tcgatattgt tactttattc gcctaatgct
     5461 cccttttaat taactatttt agcggaggat gcggaaaaaa ttcaactcat ttgttaattt
     5521 ttaaaattta tttttatttg gataatcaaa tatttactcc gtatttgcat aaaaaccatg
     5581 cgagttacgg acctataagc caggcgagat atgatctata tcaatttctc atctataatg
     5641 ctttgttagt atctcgtcgc cgacttaata aagagagagt tagtgtgaaa gctgacaacc
     5701 cttttgatct tttacttcct gctgcaatgg ccaaagtggc cgaagaggcg ggtgtctata
     5761 aagcaacgaa acatccgctt aagactttct atctggcgat taccgccggt gttttcatct
     5821 caatcgcatt cgtcttctat atcacagcaa ccactggcac aggcacaatg cccttcggca
     5881 tggcaaaact ggttggcggc atttgcttct ctctggggct gattctttgt gttgtctgcg
     5941 gagctgatct ctttacttcc accgtgttga ttgttgttgc taaggcgagt gggcgcatca
     6001 cctggggtca gttggcgaaa aactggctaa atgtctattt tggcaacctg gtcggcgcac
     6061 tgctgtttgt acttttaatg tggctttccg gcgagtatat gaccgcaaat ggtcaatggg
     6121 gactaaacgt cctacaaacc gccgaccaca aagtgcacca tacttttatt gaggccgtct
     6181 gtcttggtat cctggcaaac ctgatggtat gtctggcagt atggatgagt tattctggcc
     6241 gcagcctgat ggacaaagcg ttcattatgg tgctgccggt cgcgatgttt gttgccagcg
     6301 gttttgagca cagtatcgca aacatgttta tgatcccgat gggtattgta atccgcgact
     6361 tcgcatctcc ggaattctgg accgctgtcg gttctgcacc ggaaaatttt tctcacctga
     6421 ccgtgatgaa cttcatcact gataacctga tcccggttac gatcggtaat attatcggcg
     6481 gtggtttgtt ggttgggttg acatactggg tcatttacct gcgtgaaaac gatcaccatt
     6541 aatggttgtc gaagtacgca gtaaataaaa aatccactta agaaggtagg tgttacatgt
     6601 ccgagcttaa tgaaaagtta gccacagcct gggaaggttt taccaaaggt gactggcaga
     6661 atgaagtaaa cgtccgtgac ttcattcaga aaaactacac tccgtacgag ggtgacgagt
     6721 ccttcctagc tggcgctact gaagcgacca ccaccctgtg ggacaaagta atggaaggcg
     6781 ttaaactgga aaaccgcact cacgcgccag ttgactttga caccgctgtt gcttccacca
     6841 tcacctctca cgacgctggc tacatcaaca agcagcttga gaaaatcgtt ggtctgcaga
     6901 ctgaagctcc gctgaaacgt gctcttatcc cgttcggtgg tatcaaaatg atcgaaggtt
     6961 cctgcaaagc gtacaaccgc gaactggatc cgatgatcaa aaaaatcttc actgaatacc
     7021 gtaaaactca caaccagggc gtgttcgacg tttacactcc ggacatcctg cgttgccgta
     7081 aatctggtgt tctgaccggt ctgccagatg catatggccg tggccgtatc atcggtgact
     7141 accgtcgcgt tgcgctgtac ggtatcgact acctgatgaa agacaaactg gcacagttca
     7201 cttctctgca ggctgatctg gaaaacggcg taaacctgga acagactatc cgtctgcgcg
     7261 aagaaatcgc tgaacagcac cgcgctctgg gtcagatgaa agaaatggct gcgaaatacg
     7321 gctacgacat ctctggtccg gctaccaacg ctcaggaagc tatccagtgg acttacttcg
     7381 gctacctggc tgctgttaag tctcagaacg gtgctgcaat gtccttcggt cgtacctcca
     7441 ccttcctgga tgtgtacatc gaacgtgacc tgaaagctgg caagatcacc gaacaagaag
     7501 cgcaggaaat ggttgaccac ctggtcatga aactgcgtat ggttcgcttc ctgcgtactc
     7561 cggaatacga tgaactgttc tctggcgacc cgatctgggc aaccgaatct atcggtggta
     7621 tgggcctcga cggtcgtacc ctggttacca aaaacagctt ccgtttcctg aacaccctgt
     7681 acaccatggg tccgtctccg gaaccgaaca tgaccattct gtggtctgaa aaactgccgc
     7741 tgaacttcaa gaaattcgcc gctaaagtgt ccatcgacac ctcttctctg cagtatgaga
     7801 acgatgacct gatgcgtccg gacttcaaca acgatgacta tgctatcgct tgctgcgtaa
     7861 gcccgatgat cgttggtaaa caaatgcagt tcttcggtgc gcgtgcaaac ctggcgaaaa
     7921 ccatgctgta cgcaatcaac ggcggcgttg acgaaaaact gaaaatgcag gttggtccga
     7981 agtctgaacc gatcaaaggc gatctcctga actacgatga agtgatggag cgcatggatc
     8041 acttcatgga ctggctggct aaacagtaca tcactgcact gaacatcatc cactacatgc
     8101 acgacaagta cagctacgaa gcctctctga tggcgctgca cgaccgtgac gttatccgca
     8161 ccatggcgtg tggtatcgct ggtctgtccg ttgctgctga ctccctgtct gcaatcaaat
     8221 atgcgaaagt taaaccgatt cgtgacgaag acggtctggc tatcgacttc gaaatcgaag
     8281 gcgaataccc gcagtttggt aacaatgatc cgcgtgtaga tgacctggct gttgacctgg
     8341 tagaacgttt catgaagaaa attcagaaac tgcacaccta ccgtgacgct atcccgactc
     8401 agtctgttct gaccatcact tctaacgttg tgtatggtaa gaaaactggt aacaccccag
     8461 acggtcgtcg tgctggcgcg ccgttcggac cgggtgctaa cccgatgcac ggtcgtgacc
     8521 agaaaggtgc tgtagcgtct ctgacttccg ttgctaaact gccgtttgct tacgctaaag
     8581 atggtatctc ctacaccttc tctatcgttc cgaacgcact gggtaaagac gacgaagttc
     8641 gtaagaccaa cctggctggt ctgatggatg gttacttcca ccacgaagca tccatcgaag
     8701 gtggtcagca cctgaacgtt aacgtgatga accgtgaaat gctgctcgac gcgatggaaa
     8761 acccggaaaa atatccgcag ctgaccatcc gtgtatctgg ctacgcagta cgtttcaact
     8821 cgctgactaa agaacagcag caggacgtta ttactcgtac cttcactcaa tctatgtaat
     8881 tagatttgac tgaaatcgta cagtaaaaag cgtacaataa aggctccacg aaagtggggc
     8941 cttttttagc acgagagcct tttttgtcag ctatctatac tttaaggtga ctgccaaaac
     9001 agactcgacg tagccttcga gctgcgcacc aacacggcct cagatgggcc acatctggag
     9061 aaataccgca atgtcagtta ttggtcgcat tcactccttt gaatcctgtg gaaccgtaga
     9121 cggcccgggt attcgcttta tcaccttttt ccagggctgc ctgatgcgct gcctgtattg
     9181 tcataaccgc gacacctggg acacgcatgg cggtaaagaa gttaccgttg aagatttgat
     9241 gaaggaagtg gtgacctatc gccactttat gaacgcttcc ggcggcggcg ttaccgcatc
     9301 cggcggtgaa gcaatcctgc aagctgagtt tgttcgtgac tggttccgcg cctgcaaaaa
     9361 agaaggcatt catacctgtc tggacaccaa cggttttgtt cgtcgttacg atccggtgat
     9421 tgatgaactg ctggaagtaa ccgacctggt aatgctcgat ctcaaacaga tgaacgacga
     9481 gatccaccaa aatctggttg gagtttccaa ccaccgcacg ctggagttcg ccaaatatct
     9541 ggcgaacaaa aatgtgaagg tgtggatccg ctacgttgtt gtcccaggct ggtctgacga
     9601 tgacgattca gcgcatcgcc tcggtgaatt tacccgtgat atgggcaacg ttgagaaaat
     9661 cgaactcctc ccctaccacg agctgggcaa acacaaatgg gtggcaatgg gtgaagagta
     9721 caaactcgac ggtgttaaac cacctaagaa agagaccatg gaacgcgtga aaggcattct
     9781 tgagcagtac ggtcataagg taatgttcta atttccccgt aaagcggcaa ctttattgag
     9841 ttgccgcttt tttattccgc gcttcattct gctacagtgt ttttaa
//
LOCUS       DAJVOY010000155         9788 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVOY010000155 DAJVOY010000000
VERSION     DAJVOY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.155"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            116..736
                     /gene="fklB"
                     /locus_tag="OQL90_003275"
     CDS             116..736
                     /gene="fklB"
                     /locus_tag="OQL90_003275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1369943.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1045..2457
                     /gene="cycA"
                     /locus_tag="OQL90_003276"
                     /gene_synonym="dagA"
     CDS             1045..2457
                     /gene="cycA"
                     /locus_tag="OQL90_003276"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1369944.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(2502..3164)
                     /gene="ytfE"
                     /locus_tag="OQL90_003277"
     CDS             complement(2502..3164)
                     /gene="ytfE"
                     /locus_tag="OQL90_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1369945.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(3272..4237)
                     /locus_tag="OQL90_003278"
     CDS             complement(3272..4237)
                     /locus_tag="OQL90_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1369946.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            complement(4345..5205)
                     /gene="qorB"
                     /locus_tag="OQL90_003279"
     CDS             complement(4345..5205)
                     /gene="qorB"
                     /locus_tag="OQL90_003279"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1369947.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLTDSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            5294..5674
                     /locus_tag="OQL90_003280"
     CDS             5294..5674
                     /locus_tag="OQL90_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1369948.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(5803..7746)
                     /gene="cpdB"
                     /locus_tag="OQL90_003281"
     CDS             complement(5803..7746)
                     /gene="cpdB"
                     /locus_tag="OQL90_003281"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1369949.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIIGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            7936..8676
                     /gene="cysQ"
                     /locus_tag="OQL90_003282"
     CDS             7936..8676
                     /gene="cysQ"
                     /locus_tag="OQL90_003282"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1369950.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            8888..>9788
                     /locus_tag="OQL90_003283"
     CDS             8888..>9788
                     /locus_tag="OQL90_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369951.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLA"
BASE COUNT         2389 a         2359 c         2502 g         2538 t
ORIGIN      
        1 ttgctattgt ttctttttcc ccgggatttg tcatcattcc tgtgacaaag ttttacccaa
       61 agaagtgtgg ctgatatgct gcccgctact tttataccct aagaaaggaa atacgatgac
      121 caccccaact tttgacacta tcgaagcgca agcaagctac ggcattggtt tgcaggtagg
      181 gcaacaactg agtgaatctg gcctggaagg gctgctgcca gaagcactgg ttgcaggtat
      241 tgccgatgcg ctggaaggca aacatccggc tgttccggtt gatgtggtgc atcgcgcgct
      301 gcgtgaaatc cacgaacgcg ccgatgccgt tcgtcgtcag cgtttccagg cgatggctgc
      361 tgaaggtgtg aaatacctgg aagaaaacgc caaaaaagaa ggtgtgaata gcaccgaatc
      421 tggcctgcaa ttccgcgtga tcaaccaggg tgaaggcgca attccggcac gtaccgaccg
      481 cgttcgtgtt cattacaccg gtaaactgat cgacggcacc gtgtttgaca gctccgttgc
      541 tcgtggtgaa cccgctgaat tcccggttaa cggcgtgatc cctggctgga ttgaagcact
      601 gactctgatg ccggtaggtt ctaaatggga actgactatc ccgcaggaac tggcatatgg
      661 cgagcgcggc gcaggtgcat ccatccctcc gttcagcacc ctggtgtttg aagtcgaact
      721 gctggaaatc ctctaagcag cgcattctgt tcccctcgaa ccagagggga gcaggcattc
      781 agcaataaac ccttcagttt gccaaacggc gctattttgt gttgcaaaga ccccgtaagc
      841 gtgtattttt gtgagatgtt tcgcgttatc accgtgatat gacactcact ttaaacataa
      901 aattaacatt agatctaaat cttagtattc atcccgcgta ttgttaccta atatcgatga
      961 gtcccgatac agattcgtcg tatcatagac tgactaaagg ccgtagagcc tgaacaacac
     1021 agacaggtac aggaagaaaa aaacatggta gatcaggtaa aagtcgttgc cgatgatcag
     1081 gctccggctg aacagtcgct acggcgcaat ctcacaaacc gacatattca gcttattgcc
     1141 attggcggtg ccattggtac ggggttgttt ctggggtctg gcaaaacgat tagccttgcc
     1201 gggccgtcga tcattttcgt ttatatgatc attggtttta tgctcttttt cgtgatgcgg
     1261 gcaatggggg aattgctgct ttcgaatctg gaatacaaat cttttagtga cttcgcttcc
     1321 gatttactcg ggccgtgggc aggatatttc actggctgga cttactggtt ctgctgggtt
     1381 gtaaccggta tggcagacgt ggtggcgatc acggcttatg ctcagttctg gttccccgat
     1441 ctctccgact gggtcgcctc gctggcggtg atagtgctgc tgctgacgct caaccttgcc
     1501 accgtgaaaa tgttcggtga gatggagttc tggtttgcga tgatcaaaat cgtcgccatc
     1561 gtgtcgctga ttgtcgtcgg cctggtcatg gtggcgatgc actttcagtc accgactggt
     1621 gtggaagcgt cattcgcgca tttgtggaat gacggcggct ggttcccgaa aggtttaagt
     1681 ggcttctttg ccggattcca gatagcggtt ttcgctttcg tggggattga gctggtaggt
     1741 acaacagctg cggaaaccaa agatccggag aaatcactgc cacgcgcgat taactccatt
     1801 ccgatccgta tcattatgtt ctacgtcttc gcgctgattg tgattatgtc cgtgacaccg
     1861 tggagttcgg tagtcccgga gaaaagcccg ttcgttgagc tgtttgtgtt ggtaggtttg
     1921 cctgcggctg ccagcgtgat caactttgtg gtgctgacct ctgcggcgtc ttccgctaac
     1981 agcggcgtct tctctaccag ccgtatgctg tttggtctgg cgcaggaagg tgtggcaccg
     2041 aaagcgttcg ctaaactttc taagcgcgca gtaccagcga aagggctgac cttctcctgc
     2101 atctgcctgc tcggtggcgt ggtgatgctg tatgtgaatc ctagcgtgat tggtgcgttc
     2161 acgatgatta caaccgtttc cgcgattctg tttatgttcg tctggacgat tatcctttgc
     2221 tcgtacctgg tgtaccgcaa acagcgtcct catctgcatg agaagtcgat ctacaagatg
     2281 ccgctcggca agctgatgtg ctgggtatgt atggcgttct ttgtgttcgt ggtcgtgttg
     2341 ctgacactgg aagatgacac tcgccaggcg ctgctggtta ccccgctgtg gtttatcgca
     2401 ctggggctgg gctggttgtt tattggtaag aagcgggctg ctgaactgcg gaaataaccg
     2461 cattatcatg ctggatggcg caatgccatc cagcttttag atcactcacc cgccagcgcg
     2521 cgtgggaaca gtacattgtt ttccagactg atgtgatcca tcaggtcatc aatcagttca
     2581 ttaatgccgt tatacatcgc tttccaggtg gtgcaggctt ctggcggcgg tgtgacgtta
     2641 ttggtagtgt gtttgatcac ttccagcagt tcgcccgctt catcgtgctc gctttccatt
     2701 acgctgattg gccccattgc ctggctgccc atgccttgtt tgatcatcgg gaagaggatc
     2761 tgctcttctt tcatcatgtg gctggaaagc tcttcatgca gcatggtcag gtattttgtc
     2821 agcccttttg gcacgctcgg tttgtcggcg tgaacgcgct cgactttagt cgcttgcagg
     2881 atcagctccg gcagttgctc gcggtgacga tcgtggtagc gcacgatgat atggtcaatg
     2941 atttctgcca gcggggcgct acgccagtct ttctcaatcg gttgttcagc aagctttgcc
     3001 agttcagctt caatgacctc aacatccagt tctttacgtg ccgccgcgcg cgccagcgtc
     3061 tgcttaccgc cacagcagta atccatatta tatttacgaa acagagctga agcgcgagga
     3121 atagagagcg ccagttcacc taaaggttgg tcgcgataag ccatagctga tacctcattc
     3181 ttaataagat aagatgtatt ttaaatacat ctttaaggca aaagctataa cccttacgta
     3241 gcagaggctt atttaactca cagccagtat gtcaactttc tgagacagat tcagttaaag
     3301 gcttttccgg ttttgctctg actacaatga ccacgccaac cactaacagc gcgataccgc
     3361 tcagcgtcat cagcggcggc atttgctggc gtatcaaaaa ggtgtacagc aaacctgcca
     3421 gtgtttcgaa aacgatcagc ggcccgagaa tcactgtcgg taatcgctgg ctggcgacgt
     3481 tccagcagag tgcgccaacc catgagcaaa gcacggctat cgcaaccatc agactaataa
     3541 acaccagcgg acgggggcca aaaggtaggg agaagtccgg cgtttgcgta ttcagccagt
     3601 aacaggcgac gagatagccg atgagagaaa ccggcagtgt gaccagcgcc tgcgccgtcg
     3661 cccacatcat cggatgtttg tcgggattct cccgcagcca gcgggcgttg cgcagggcat
     3721 accatgccca gcagaccacg gaaactaacg ctagcacgat gcctgaggta taacgtgccc
     3781 agtcaaaatc ggggagtccg tggtttaact cagcaatatt cacacacgcc aggccgatgc
     3841 caatacaaac cagtgccggg gcgagttttc cccacgcgag tttgccgtcg cgctggctat
     3901 aaagcagatt ggcaaaaacg ggaatcacca ccggcagggt gccgataatc atcgtggaaa
     3961 caggcgcacc agtacgttga atggcactgg caaggcagaa gtaatagatg aggttgccca
     4021 tcatagtgag catcaaggcg gtaagccagt cgcgacgcgc caactgacgc agacgcacgc
     4081 gtcccagcca ggcaatgggc agcgcaatta accctaacgc cagataacgc cccatcgact
     4141 gcaacatcgc cgggtattcc ggtacgatca acggcccgac aaaaataagc ccccacatca
     4201 accctgctaa cagggcgtac agcacgccgc taatcattac tggcatccat tggtctgtca
     4261 gaagaaagtt caggaagcag acatgattgc tcccggcatc ccgtcattat tagagaggat
     4321 gcgggaggat gccactttaa ttaactagtt attaacatta aaaagatggc ttacgctttc
     4381 ggctaacgtt gtcgttgggc ggccaatcaa tttgctaagc gttttgctgt tatcaaacag
     4441 accacctttc gatgcgccaa cgtcagaatc cgtcagcata tccgccagtc cgtcgggcag
     4501 tccgacgctt ttcagtgccg cggcgaaatc ggcttcgctc agattttgat aggtaacctg
     4561 tttgccgctc tgtttggtca gctctgccgc taactgtgtc aacgtccagg cactatcgcc
     4621 cgccagttcg taaaccttgc cttcgtgacc ggcttcgcta atcacgcgtg ccgcagctgc
     4681 cgcataatct gcccgcgttg ctgaggcgat tttgccatcg cccgccgcac cgataaatac
     4741 gccgtgttcc agtgctgccg gggcgctggc gaggtagttt tcgctgtacc agccgttgcg
     4801 cagcagggtg taaacgatgc cagaatcagc caacattttc tccgtctcaa tgtgctcatc
     4861 ggcgaggccg agcggggagg tatctgcatg tagcaggctg gtataagcga taaatttcac
     4921 gccagccgcc tttgcggcat taataacatt acgatgctgc ggggcacgtt gacccacttc
     4981 gctggaagag atcagcagta gtttttccac tccctgaagt gcagatgtca gtgcggcttc
     5041 atcgccgtag tcagcctgac gcacggtaat gccttgtgct gccagagcct gggcttttgc
     5101 cggattacga acgatagcca ctatttggct ggcaggaacc gttttcatca aggattcaat
     5161 aatatagtga ccaagttggc cagtggcacc agtaatagcg atcatgggaa gtctccatcg
     5221 ttttgtcttg tgtttaaata cgctaacacc taaacttact tttagtaagt acgtacaaaa
     5281 aggtaagtat gaaatgagtc aggttagcct gtcgcaacaa ctgaaagagg gtaatctctt
     5341 tgcggaacag tgcccgtcgc gcgaggtgtt gaaacacgtc accagccgtt ggggggtgtt
     5401 gattctggtg gcgctacgcg aaggtactca tcgctttagc gacctgcggc gcaaaatcgg
     5461 cggggtgagc gaaaagatgc ttgcgcagtc gttacaggcg ttagaacagg atggttttct
     5521 taaccgtatc gcgtatccgg tggtgccacc gcatgtggaa tatagcctca cgccgttggg
     5581 cgagcaggtg agcgaaaagg ttgctgcact ggcggactgg attgagttga atttgcccga
     5641 ggtgttggcg gtgcgggatg aacgtgcggc ataactgccg gatgcgctga gcttatctgg
     5701 tctacggtgc atggctggta gggcggataa gatgcgccag catcgcatcc gccaataaat
     5761 gccggatgcg gttgataatt tatagaccga aaaagaagtg ttttacttac tcaaatccac
     5821 ctgataaatc gcaaacccga tatcatcggt cgcgacttta ttcatcggat attgcccttt
     5881 ctctttaata aacgctgcgg ctttatctga cggggaagtt tcgaaacgga tatccagttt
     5941 cttatcgcca gctatcggtg ctaaacgcca gttgttatct gccgccgggt gaatttctcc
     6001 cgcacgtttc gactcatcag caatccacgc tgtcagcacc gagcggttct catccggtga
     6061 agcaaaagcg atatgtctgt cgcccgtacc ggcaaatttg ccgccgtaag cgcgatagtt
     6121 attggtggca acgaggaaca tagcgttcgg atcaatcggc ttgccattaa aggtcaggtt
     6181 cttaatccgc tctgcattgg cattaatcat ctggcactcg ccgtcatagc gggcgggttg
     6241 ggtaacatca atctgataat tcacaccatc aataacatca aagttataag tgcggaaacc
     6301 atcccagttg atgagtgact gtggtttcgt gctgttagga tcaatctggt taaactgtcc
     6361 ggcggagcac tccagccact ctttcacctc tttaccgctg gctttcacta caatcagcgt
     6421 attggggtag agataaagat cggcggcatt acggaaggtc aactggcctt tttccacctc
     6481 cacatagctt gccgggtcat ttttgcgacc accaacttta aacggtgcgg cagctgaaag
     6541 caccggcagt tttgccagat ccggatcgcc ctgaatgtaa tgttcgacat acgctttttg
     6601 cgcgttgttc acgacttgca cggtcggatc gtcctgcacc agcgccagat agctatacat
     6661 attgtcggca gatttaccga ttggcttgct gacgaactgg cgtgtggcat cgtgatcggc
     6721 tttgagtgtt tctaccagct tgctgtcttc cgccgcgagg gattttttat tggcgatgtc
     6781 gtaaatcggc cgtgcttccg ctttcgcctg cgtcacctgc catttaccgc tgtcattact
     6841 gagttgtaag tcgaccaccc caagatgatc gccccacatg cccggcatta ccgccggaac
     6901 accattcagc gtgcctttgg tgatatcagc ctcttcgata tcagcaaaat ctttacccgg
     6961 gaaaacggcg tgagcatggc caaacataat ggcgttaacg cccggaattt cactgaggta
     7021 ataaactgag ttttccgcca ttactttata cggatcggca gacagcccgg aatgcgccag
     7081 aacgacaacg acatcggcac ctttctcgcg catttcaggc acgtatttgc gcacggtttc
     7141 ggtaatatca ttcaccgtca cttttccgga taaattagct ttatcccagc ctatgatttg
     7201 cggcggcacg acgccaatat agccaatctt cagcgtctgt ttttttccgt ctttatcgac
     7261 cacttcggta tctttaatta aatacggtgt aaacattggc tgtttggttc tggcgtcaat
     7321 gacgttggca tttacataag ggaatttcgc tcccgccagc gcatttttca ggtaatccag
     7381 accgtagtta aattcatgat tgccgagtgt accgaccgta taatccagcg tattcagcgc
     7441 cttataaacc ggatgaacat cacctgcttt taatcctttc gccgacatgt aatcggccag
     7501 cggactcccc tgaatcaaat cgccgttatc gaccagtacg ctgtttttca cttcattgcg
     7561 ggcatcgtta atcaggcttg ccgtacgtac cagtccgaat ttttccgtgg cggtgtcttt
     7621 gtaataatcg aaatccatca tgttgctatg cagatcagtg gtttccatga tacgcagatc
     7681 gaccgtcgct gcattcacac tggcggcaat cagcgtggcc aggagcgttg cgctaaactt
     7741 aatcatcagg gacatccttt tatcatcggg aatacgaaag aaaagggaga ataaacgtct
     7801 tacttataga acagtgaaga atgccacaat tttacgcttt gaaaatgatg acactatcac
     7861 agttggcgca ttcattaacg atagggtata agtaaaacaa taagttaaca ccgctcacag
     7921 agacgaggtg gagaaatgtt agatcaagta tgccagcttg cacggaatgc aggcgatgcc
     7981 attatgcagg tctacgacgg gacgaaaccg atggacgtcg tcagcaaagc ggacaattct
     8041 ccggtaacgg cagcggatat tgccgcacac accgttatca tggacggttt acgtacgctg
     8101 acaccggata ttccggtcct ttctgaagaa gatcctcccg gttgggaagt ccgtcagcac
     8161 tggcagcgtt actggctggt agacccgctg gatggtacta aagagtttat taaacgcaat
     8221 ggcgaattca ccgttaacat tgcgctcatt gaccatggca aaccgatttt aggtgtggtg
     8281 tatgcgccgg taatgaacgt aatgtacagc gcggcagaag gcaaggcctg gaaagaagag
     8341 tgcggtgtgc gcaagcagat tcaggtccgc gatgcgcgcc cgccgctggt ggttatcagc
     8401 cgttcccatg cagatgcgga gctgaaagag tatctgcaac agcttggcga acatcagacc
     8461 acgtccatcg gctcttcgct gaaattctgc ctggtggcgg aaggacaggc gcagctgtac
     8521 ccgcgcttcg gaccaacgaa tatttgggac accgccgctg gacatgctgt ggccgcagct
     8581 gccggagcgc acgttcacga ctggcagggt aaaccgctgg attacactcc gcgcgagtcg
     8641 ttcctgaatc ctgggttcag agtgtctatt tactaaattc agatggcaga aacagtgtat
     8701 ttcctgattc tgccatcctg atttctccca acctaaaaag ttataaataa aaagagattg
     8761 tatttaaagt gcaaaaattc aattgctaat aagttacatt ttaataatga gcgttttttg
     8821 atagtttact tctataatga gatatttaat ggcgacataa aataaccaaa taaaataagg
     8881 tagccatatg ttacccagga tcagacacaa taattttatt ggtgcggtgg agttattcgt
     8941 aaaatcatcg catacaaaaa cacattcaaa caattttttt aataatattc agcatgcatt
     9001 taagaaaaaa gattgggttt cgaattatga tagcctgtta actttgtggg agttttttcg
     9061 ttgcgcaact cagattgata aagatagtta tcaagtgctt tcatcaaaaa atgaaactgt
     9121 taaagccatg gataagtttc ttatcagttt cagccttaaa gataacggtg ccgaatatac
     9181 aatgacattg cgaggcagtg gatttgaata cgaagaaatc cccataacaa taaatgagta
     9241 taactcattc atggatttca aaaaccgtga atttccgtta gaacaaaaca gaagattgta
     9301 tgcttgggat attctgcaga aaaaacagtc tgacataccg aaaaggatta aaggttatat
     9361 tcaccaggcg attggtgacg tgtctttagg ttatgcctta ttggaggata tagtaagcaa
     9421 attaaaacgg ggtaaatttg aactccaaat accaggaggt ggaattaaag aatgcgatgg
     9481 atggtatatt tacgaaaaaa taattgatga taattttgcc atagtcatag aatctttggg
     9541 ctttgcgctt aaaatctatg gaggggatga acgttttcgt aatggttcct ctgttgtttt
     9601 ggaggatgaa gattactctc ttatttataa ttttctggtt aatgcaggtt gccaacaagt
     9661 cgaattagcc gaacaagtgg atgcgatagt aagtgcgaat ctggccgtag atagtgatat
     9721 taccgaagag gagatttgcg agaaatataa atcaaccatt gaggcgttta aaaaagagca
     9781 actagcgt
//
LOCUS       DAJVOY010000156         9772 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVOY010000156 DAJVOY010000000
VERSION     DAJVOY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.156"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(266..1609)
                     /gene="gdhA"
                     /locus_tag="OQL90_003284"
     CDS             complement(266..1609)
                     /gene="gdhA"
                     /locus_tag="OQL90_003284"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS1369952.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVGAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1845..2117
                     /locus_tag="OQL90_003285"
     CDS             1845..2117
                     /locus_tag="OQL90_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1369953.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2083..2490)
                     /gene="nudG"
                     /locus_tag="OQL90_003286"
     CDS             complement(2083..2490)
                     /gene="nudG"
                     /locus_tag="OQL90_003286"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1369954.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2577..3188
                     /locus_tag="OQL90_003287"
     CDS             2577..3188
                     /locus_tag="OQL90_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1369955.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3197..4504)
                     /gene="ynjE"
                     /locus_tag="OQL90_003288"
     CDS             complement(3197..4504)
                     /gene="ynjE"
                     /locus_tag="OQL90_003288"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCS1369956.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4571..5224)
                     /locus_tag="OQL90_003289"
     CDS             complement(4571..5224)
                     /locus_tag="OQL90_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1369957.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5224..6759)
                     /locus_tag="OQL90_003290"
     CDS             complement(5224..6759)
                     /locus_tag="OQL90_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1369958.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCTLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALAAKWVGYVRQ
                     GLR"
     gene            complement(6732..7898)
                     /locus_tag="OQL90_003291"
     CDS             complement(6732..7898)
                     /locus_tag="OQL90_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1369959.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7908..8456)
                     /locus_tag="OQL90_003292"
     CDS             complement(7908..8456)
                     /locus_tag="OQL90_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1369960.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8456..9163)
                     /locus_tag="OQL90_003293"
     CDS             complement(8456..9163)
                     /locus_tag="OQL90_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1369961.1"
                     /translation="MMKMQSRKIWYYCITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9178..9772)
                     /gene="ydjY"
                     /locus_tag="OQL90_003294"
                     /pseudo
     CDS             complement(9178..9772)
                     /gene="ydjY"
                     /locus_tag="OQL90_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjY"
BASE COUNT         2325 a         2670 c         2522 g         2255 t
ORIGIN      
        1 atatctgctg ctatcgcaga aagttaacgg atgctgaact tgaagcgtgt tatcgttacg
       61 acaagcagtt ttgggagcac tacagtaaga aatgccgttg gcccataaga aatgttccct
      121 ggggagcatc atccgttaaa tactcataaa cgcctgaaat tttgccgggg gcgctgcgct
      181 tgcccggcct acattagagc gtaactgcaa tgaattgtgc ccatttgtag gcctgataag
      241 cgtagcgcca tcaggcattt acaacttaaa tcacaccctg cgccagcatc gcatcggcaa
      301 ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt ggtttgctca ccttcaccac
      361 catgctcaac acaggcatgg tggatatcca gcatgatgtg atgcaaacgt gcgtcaactt
      421 tctcggcttt ccagcccagg cgcgcagcgt tttgcgccat ttcaaggcct gacgttgcta
      481 caccgccagc attagccgct ttacccggtg caaacagtac gcctgcctgc tggaacagtt
      541 cagtcgcttc gatggtggtc ggcatatttg ccccttcggc gacggcttta acgccattgg
      601 cgataagctg atgcgcggcg ccaacatcca gttcgttctg ggtggcgcaa ggcagggcga
      661 tatcaaccgg tacagaccac ggctgttggc cttcgagata gaccagacca aattctttgg
      721 cgtaatctgc cactcgacca tcgcggctgg ctttgatttc gataagacgt gccagtttct
      781 ctttcgtgaa tccgctttca tcaactacag tgccgctgga gtctgacgca gtgatcacac
      841 gagcaccaaa ttccatcgct ttttcgatag cgtactgggc gacgttgccg gagccagaaa
      901 cggaaacgcg catcccttca aaacccatac cgtggcgttt taacattgct tctgtgaaat
      961 aaaccagacc gtagccggta gcttccgggc gaataagact accgccaaat gaaaggccct
     1021 taccggtgaa gacgcaggcg gtattgttgg agagcttttt catcatcccc gccataaagc
     1081 cgacttcacg accaccaacg ccgatatcac ctgccggaac gtcagtatcc gcgcccagat
     1141 gacgataaag ctcggtcatc agcgcctggc agaaacgcat cacttcgctt tcgcttttac
     1201 ctttcgggtc aaaatcgcta ccgcctttac caccgcccat cggcagggta gtcagggcat
     1261 ttttgaaggt ttgttcgaag ccgaggaatt tgagaatgga gaggttaact gacggatgga
     1321 agcgcatacc gcctttgtac gggccgatgg ctgagctgaa ctgcacacgc catgcacggt
     1381 tgacctgtac ctggttgcga tcatcaaccc ataccacgcg aaactggatc acgcgctccg
     1441 gttcaaccag acgctccagt aatgacatct ggcgatattt tggattttgt tcaagaaaag
     1501 gccagagtgt ggtcattact tcacgaacgg cttgcgcgaa ctcggtttga ttcgggtcgc
     1561 gcttttggac atggttgagg aatgactcca gagaatatgt ctgatccata gatataaaac
     1621 ccttatatat taatacgatt gtgcttatgt tgtttatgtc atgtgctttt gcagttttcg
     1681 actataccat caagaatgtg acggtaagca agaaaaaaat gacccaggaa agcaaattaa
     1741 taacgagagt aatctcataa ccaaatgcga taactcagac gctaaagttt tgccacccgg
     1801 ctaccgttat cagttgcaga gacaataacg ttaaggagtt ccctgtgagt cgagcattgt
     1861 tcgccgtagt attagcattt ccattaattg cactggctaa cccgcattat cgtccggacg
     1921 tggaggtaaa cgtaccgcca gaagtgttca gttcaggcgg gcagtcagcg cagccctgta
     1981 ctcagtgctg cgtttatcag gatcaaaatt attctgaagg ggcggtaatt aaagccgaag
     2041 gaatactgct gcaatgccag cgggatgaca aaacccttag cactaatccg ctgctctggc
     2101 ggcgcgtaaa gccataaacg cctctaatag tggaatgtca gtaggggcca gcggatattg
     2161 cagcgcctct tcaggtgagc accagaccag cgcctgatgt tcatgtgcct gtaacgtccc
     2221 gtggaagtcg ggtacgtgcc aggcatgaag atggataatc cggcccgaaa cttctcgctg
     2281 atggctggca acatactcac ccacagttgc ttcgatgccc aattcttcgt ttaactcacg
     2341 caccagtgcc tgctgctggc tttcatccag ctcgacttta ccaccggcaa actcccataa
     2401 tcccgcctga tcgctctggg cggggcgttg cgcgagtaaa attttgccat cacgttcaat
     2461 gatggcggca acaacttcaa tcattttcat aaattacaca cgggcagata agcgaaaaat
     2521 gccataatat ccgcactgtt gctcaatcag aaaggcttta tatttaagga gtgcctgtgc
     2581 tagaccgcca tcttcatccc cggattaaac cgttgttgca tcagtgcgtg cgggttcttg
     2641 ataaaccggg cattacgcca gatggtttaa cattagtggg atttgccatc ggcgtgctgg
     2701 ctctgccgtt tctggcgctg ggctggtatc tggcggcgct agtcgtcatt ttgttgaaca
     2761 ggctgcttga tggtctggac ggtgcgctgg cgcggcgcag agagcttacc gacgcgggcg
     2821 gctttcttga tatttctctc gattttctct tttacgcgct ggtgccgttt ggctttattc
     2881 ttgctgcacc ggagcaaaat gcgctggcag gcggctggtt gttgtttgcg tttatcagca
     2941 cgggcagcag ttttctcgcc tttgctgcac tggcagcaaa acatcagatt gataaccccg
     3001 gttatgcaca taagtcgttt tactatctgg ggggattaac cgaaggcaca gaaacgatct
     3061 tactgtttgt gctgggatgt ttattccctg catggtttgc gtggatcttc ggtgcgttgt
     3121 gctggatgac gacctttacg cgggtgtgga gtgggtatct gacgctgaag tcgctccagc
     3181 gtcagtaatt gcaatgttat ttgctactgt ccgggccgcg ttcaccggtt gctaccggat
     3241 tttttggatc gctgctccat tcgtaccagc cgccgtcata cacggaaaca ttcttccaac
     3301 ccatggcgcg tgcgtacata aaggtttcgg acgcgcgcca gccggtgccg cagtagaatg
     3361 aaacttgctg ctctggtttg atattccacg ctttccacat agcggtaata tcatcggcgc
     3421 tgcgcatggt gccatccggg ttatgaaaat cttccatatg cgtcgagtcg ctaccagcgt
     3481 gtccccaacg tgctccggct atttcacctt ttggtttaat atagctgtaa ccgctggtcg
     3541 taccgataaa ttctggccac gaacgaatgc tcaccagcga tgcatcctgg cgatgcagca
     3601 gtccacgggc ctgttccata tcgagcatca actgcggttg tgccgggatc ttcacgccga
     3661 aatccggttc cgctttcact ttcggtggcg ttcctcgctc aacaggcagt cccgcgtcgg
     3721 accaggtttg ccagccgcca tccagcagac gcacatcttt cacgcctgcg tagagcataa
     3781 tttgcgccac acgcgctcca gcgtatacgt cacgcccgta cagaatgacc gtggtgtcat
     3841 ggcgaatgcc gtgttttgcc agcatcgctt ttagttgttc atcagaaact ttgttccaca
     3901 gcggttcgct ttccacttcg ttggtatcga tgtagtcagc accgggaata tggctgataa
     3961 ggtaaagctt aggagcgccc caggccgctt caatgacttt ccagtcaccg gcaggtttcg
     4021 ccgtaacctc tttaccttgt tgcaggtcgt gcagccattg cggataaacc agctgctcaa
     4081 agtgcggcag tttttgtaga cgggaaggtt cgcttagcgc gtcactcaga atggagatat
     4141 gcgtaaaacc tgctttttgc agtcgcgttt tgacggcgtc gacatctttg tcattaccgt
     4201 acagcgccac cggggcatcg gctttcaggt tatgttgctt gatccacgcg ttgagctgtt
     4261 cggtgctcat tttgtcgagc cagctggcag agaggtttaa ggcggcaggt tcatgaccag
     4321 aagggccatt taaggtttgt ggccagccgt tataaaacgc gctggggcga gtatctatcg
     4381 ctttgccatt ttgttgttga agctggtcaa gtgtaagagg cttcgccagt tcagcggccc
     4441 acgaagaagc gcaagccagc cctaaagcca ttgccagcgc ggtcatttga gaaacacgtt
     4501 tcatcgaaaa tcccgacatt gtagttttga gtgcgccatt ctgaactgac gcattgagaa
     4561 aaactttgcg ttatcgcagt ttgttgtaat tttctgacca ctgcgccata tccagaacag
     4621 aactatcagc aggaacatcc tggagatcgt gcgttacctg aacgacgggg atcgccagtt
     4681 cgcgaacttc gctgaacacc cactggcgaa aattatcgcg cagagccaca tcaagacggc
     4741 tgaatggctc atccaggagt aacgcttttg gctgagcgag aagggcgcgc agcagggcca
     4801 cccgcgctcg ctgaccgcct gataatgtcg cagggtcctg gtgataagtt tcagccagtc
     4861 ccgctcgatc aagcgcgtct ttcacggcat tgcgcctggc agtccccttg agcgtcgacg
     4921 gtagcgccag cagtaaattt tgcccgacgc tgaactggtc aaataacagt gcatcctgga
     4981 aaagaatgcc aatctgacgc tgtgcggtgg gtagcatgtc aatccgttgc tcattgagcc
     5041 atagctcacc tgtacaagaa aactgtccag ccagtgcacc aatcatccat gaaaacagag
     5101 tggattttcc acagccagac ggccccatta acgtgacgat gtcaccttta tcaaccgtaa
     5161 agttaacgtt tgtcagcaag cggctttctg gtaaacgtag cgaaacattt ttcacgcaga
     5221 gcattagcgg agtccttgtc tgacataacc tacccatttt gcggctaacg cggtcagggc
     5281 aaaaataata agcggtaaca acagttgcca taaagcctgg gcggcgagaa taccgttgct
     5341 gccgccgctg cttaatgcca ccgcctcagt ggtgagcgtc ggaaaacgcc ccgcgcccag
     5401 ccacagcgtc ggcatatact gcgcaatact gactgaaaat cccaccgcga aggcaatcag
     5461 cacagggcgc aacatgagtg tgcatttcac gtaaaagaag attttggccc gcgaccagcc
     5521 cagtgtttgc gcaatcaaaa ttaaccgtga atcaatgcgc tgccaggcgg gttgcaggat
     5581 aaacagcatc cacggcatta cccacagcag atgcccccag accaccgccg tccagcttcc
     5641 atccaggttc agccatagcg ccagcgtgta ctggcccgcc accagtggca gagcgggcaa
     5701 tagaatgggc aaccatagcc acaactggcg acgctgtggc ccccattcca gccacagcag
     5761 taacaggagc aaagcgatga atgttgctac cagccccatt gtcagactgt tgatgagtgc
     5821 ttcactattg atcgtcgact gatccgcgag gatcgccagc agaaccacac acagcacacc
     5881 ggttaagggt aaaaaactcg ccagcgtagt gcctggtaat aaaggtgtgg caggcttgcg
     5941 aatgccatct acgcggggaa tagtgcgccg ccagctgcgc catagcagat agctcagcaa
     6001 aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt tcgtttgttg
     6061 atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg gtggattacc
     6121 tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa tcgccagcat
     6181 cgccattgcc agcgaaggcg cgacggaggg caacagcagc cagttcaggc attgccagcg
     6241 gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt cgctcaatac
     6301 tgccgctaag atccacagca gaaatgcgct ttctttcact gcaagggtga ggcccagacc
     6361 gatgccaaat cggtccattg gcggagtgaa atacgggaaa tagtcatata gcagccctcc
     6421 gtcagcaaag agcagcaggg cgctggtggc aaaagccaca tggggaatgg cgagcagcca
     6481 cggcagacgg gcgcacatac gctgccattt cggcccaggc catagcgcca caatcaccag
     6541 cagggcaatc aacaatgccc cgaccgccgc gatggttgtc gacaccagcg ttgccagtaa
     6601 tgcatgcggt aactgtggat cggcaaataa cgcctgccag tgtgtcaacg acaacgcagg
     6661 cgatatcagc gtgagagctg ccgggatcag cggtgcataa atcaccgcca ccatcgccca
     6721 cagcaaaaag attaatgcgt accgtaacgg cgtaaccatt cttgttccag tgcattcacc
     6781 catcctgcgt gcggttcagc cagtaccggc ggcagatcct gcggcattct tgattgcaat
     6841 atttcgcgct gcccgtcagg cagtttttgc ggatcgagaa cagaaggatc gtcccagaca
     6901 acgggatctg ctttacgcag ttgcgcatcg ggtgagagca gaaaattggc aactaccttc
     6961 gccgcagcac tggcattcgc gttggctgga atggtgacga aatgcacgtt gccaatcatc
     7021 ccctcgcgaa agccaaaact gtaactgctt gccggcaaat cgccgctggc gattttttgc
     7081 tgcgcatgcg cggggttaaa ggtcagcgac aggcgcaatg tgccggcttt cagcagagca
     7141 tccatccgcg cgggcgaagg cgggaaatcc tttccttcgc gccacaaata cggatgcagt
     7201 gcatcaagat attgccacaa gggcgcagtg acacgggcga aagtcgcatc gtccggcgct
     7261 tcttttaatg cggcgggatc tggcgtcagc ataatcagca actgttcaag aaacgccgtg
     7321 ccggtaaagt ccggtgggcg cggataagta accgtgccgg gattggcttt agcaaactcc
     7381 agtaaggctt gcggcgtttg tggtggctgt ggcgtaacat cgcggcgggc gataaacgtc
     7441 agttgtgcgc cgccccaggg cgattccgca ccttgtgtcg gcactgaaaa atcttcccgc
     7501 accggcagct gtgtgtcgac atagcgccag ttgggcagag tctccgccca gcccgtttgt
     7561 aataaattgg cctcttttaa ggtgcggaag ttttcgccgt tcacccagag cagatccacc
     7621 gagccgcccg ttttacgacc ggcagcagct tcggtctgaa tgcgcttcac cgcgtctgcg
     7681 gcatccgcga gacgaacaat cttcaggttt atagcgtaat gggttttcat ctcgccgcta
     7741 acccagtcga gatagcggtt aattgcggta tcgccgcccc aggcgttaaa ccagacggtt
     7801 tgccctttgg cctcattttt aatttcctgc cagtcactgg catgtgttgc caaagaaaac
     7861 agcgataaca atcccagcaa ccacccacaa tggcgcatct tctctcctta agaacgtttt
     7921 tccggaatac ggcacagccc ctgagcggga atgtccatgg cagaattaaa acgagccgac
     7981 aggttttgca ggccgatcaa tgccgtcagt tcggtgagcg cctgagcgtc aaaatgcgca
     8041 gccagtcggg tacgcagggc atcatcgact gttggcggtg tgacgcttgc ggcttcggcg
     8101 tactccagcg ccagccgttc ttcatcgcta aagagcgggc tttgccgcca gtcagccact
     8161 gccagcagtt tatcggtgct gccggtacgc tcggcgactt tcatactggt gatatccaca
     8221 caaaactcac acaggcacat ttgcgcaatg cgcgcgctga caagcgatcg cactaccgga
     8281 tcgagcggtg agcgtttgcg ctccagccac ccaacaaaca ttgacaccaa ataaaagata
     8341 aacgggatcc gcccccacca gcgaatcggg cttagcacct cgccgtaatg gcgacgatgc
     8401 agccacgcct gcggacgtaa aatgaaagga attttgctaa gcggcggtaa acccatcagt
     8461 cattcctctt ctggcgctca agccatattt tcttcgccat aaaaatcacc acggtcaggg
     8521 caaacagaat aaacagtccg gtgacaaacc agaaagtacc gcctgttaac atgctgcccg
     8581 cccaggaata aacaatagtc gccggtaatt gaccaagccc ggtggcgata aaaaacgagc
     8641 gaaaacgtat tgaagtcaga ccggcagcat agctgattgg atcgaaaggg acaaaaggca
     8701 ataaccgaca gaccaggatg gtgtgtttgc cgtagcgagt gaaaaagccg tccatactgt
     8761 caagcacggt tttgccggtt aatttttcca ccacttcgcg gcccatcact ctggcgataa
     8821 aaaagcacag cgccgcgccg gccatcgaac tggtccacga cagcaagccg ccccagaacg
     8881 cgccaaacag cgacgcattg gcaaaggtga tcaaaaacgc aggtagcggt gcagcaatgg
     8941 cctgtaaaat catcaacaag aacgagacaa ctgccgccag tgcgccgtaa gactgaataa
     9001 agcgttctat accctgttgg tccacggcgg caaacgccgc aacgctgcga ttgataaact
     9061 catggacgcc gggaagcagc gcccatgcga gcagcatagc gaacaacagg atgatgaggg
     9121 ttatacagta gtaccagatt ttacgcgatt gcatcttcat catcctttat cctggcttta
     9181 ttcggtgatt ttaaaggtaa ccgttgccag cgtgttatcc gccgggagaa ctgaggcatt
     9241 gcctttgaat ttcacttcgc cgcgtttttc caccgcacca taagtgtatg ttgcattgct
     9301 gacgatgccg accgggcagc tatccagaca caccagtcaa cccgttttct tctcttctgc
     9361 tgccgttaaa ttaccgccaa agcgcatgtc cagtttcttg ccattactgt caacaatcac
     9421 ttcatcgaag gaatacgctt ttgccgcccc ttgccagttg accgaaatat ccagtttgct
     9481 gcctgtgaca tgagtcgttt ctttattatc catcgtcatg ttttcgcccg gcgtaccacc
     9541 tgcctctttc agggcttcat aaaatgcttt cggggtcgca taagccataa acagcgattt
     9601 atgtccgttg gagccatctt taaacacaat accgtgacga gtgtcgtcgg tgaggaaacg
     9661 accattaatt tgcaccagca tggttaccgt atggttattg gcatcgacgc gcagcggttg
     9721 gctgttcgaa agtccgtcgt attccacttt tgccgccgaa ttctcttttt ga
//
LOCUS       DAJVOY010000157         9626 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVOY010000157 DAJVOY010000000
VERSION     DAJVOY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.157"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(190..531)
                     /locus_tag="OQL90_003295"
     CDS             complement(190..531)
                     /locus_tag="OQL90_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1369962.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(534..4304)
                     /gene="tamB"
                     /locus_tag="OQL90_003296"
     CDS             complement(534..4304)
                     /gene="tamB"
                     /locus_tag="OQL90_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1369963.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAQEEVVEPKIENPQPDEKPLGETLKDLFSRPVL
                     PEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDSNQG
                     IVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGPVDM
                     DLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMRTAV
                     KGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTLNGI
                     NTAKEFPEWPSKLNGLIKTSGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGNSYM
                     QWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKVRGT
                     VEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNINLV
                     TLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPWSLT
                     RDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLKPFM
                     PETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTLNLT
                     AELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIFTRG
                     EKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMRSTL
                     AGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFEATP
                     NLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNLIVH
                     VGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGELLF
                     SGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSYLLR
                     GQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQVVVS
                     GYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4301..6034)
                     /gene="tamA"
                     /locus_tag="OQL90_003297"
     CDS             complement(4301..6034)
                     /gene="tamA"
                     /locus_tag="OQL90_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1369964.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6240..6878
                     /gene="msrA"
                     /locus_tag="OQL90_003298"
     CDS             6240..6878
                     /gene="msrA"
                     /locus_tag="OQL90_003298"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1369965.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7201..8544
                     /locus_tag="OQL90_003299"
     CDS             7201..8544
                     /locus_tag="OQL90_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1369966.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8606..8812)
                     /locus_tag="OQL90_003300"
     CDS             complement(8606..8812)
                     /locus_tag="OQL90_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1369967.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9137..>9626
                     /locus_tag="OQL90_003301"
     CDS             9137..>9626
                     /locus_tag="OQL90_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1369968.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAK"
BASE COUNT         2212 a         2647 c         2369 g         2398 t
ORIGIN      
        1 catactcacc gttcaaagcg tgcccccgtt ttacccctgc acatttgtga acgccagcag
       61 agttttttat gcgattgcag atatggaaag cggctcggag agaaaagcaa aagatggaaa
      121 agttacaaaa aaaaatcgcg agatacatcg cataaaacaa cgccacccga aggtggcgtg
      181 cttttattat tacttatccc tgtctaacca gtcgccgctt tcaattagct ttaatccctc
      241 gacgggtcgt tgataaacgt acatccatgc actcccatac ggcgtctgaa tcaactggcg
      301 cgcgtattca ccgcccctgg tgcgcaaggc atcaagttcg gccagcgtgg cgttgtcaat
      361 acgataaact tcaccgtgta ccgttccgtt ccccggaact gcgcctggat agtggcccag
      421 gctatacaac tggtagttat cgatactgaa atcgcccagt aactgggcat tggtcatcca
      481 gtgactgttg ccttgtttgt ggcgtaaact gccgtagaca aatattcgca ttgctaaaac
      541 tcgaactgat agagcaaatc cagtgcctgg tctacaccag acacggcttc cagatatagc
      601 ttaggcatca ggcgataacg taacgtgagt gttgctatag agtcaaatat acccacgccg
      661 tatttcactt gcaggcctgg caatacatag ccgctgacca ctacctggga ggagtcgcct
      721 actccctggg tgtcgagcgc taaattgctt acgccaaacg tctcgccgat tttacccaca
      781 atctggccac tttgcgcaac ccccagacca atcagcatcg aggtcattgc cgcactgtcg
      841 ctctgatcgc tctccagccc ttgtccacgt agcaaataag acaatgcagc ttgttgcgac
      901 atcgccgggt cagagaagat ctccgctttc ggttcgtccg ccagaccagt gacgcgaact
      961 ccggcgatta cgtcgtcttc tgtagcatcc gggttacgaa tagcttcaat attaagatac
     1021 ggttgatctg gcggaccaga gaacagtagc tcacccttac gcacaatcag atcctgacca
     1081 taggcatgga agcgcccttc agggatgttg atctgcccgt tcaggcccag cccttgtttg
     1141 tcctgaacga cgttaagatc gcccgtcagc cgcgctttca ggccaaaggc gtcaatgcgc
     1201 acattgttgc caacgtggac aatcaggtta ctgttaatcg gaatcgacgc cgttttcggc
     1261 tcttccggtt gcaggttatc gttaagcatc accacatcgc tggagacgcc tactgcgctt
     1321 tccggcagat cgtgcaccac gatgcgcgcc cacgggacat ccacgcgacc atcgagggta
     1381 aacaggtttg gtgtagcctc gaatacaaca tctggcgata catccattcg taccatcggc
     1441 ggcacggtga tccgcacttt actgcctttc gccgttactc gtgcccgcca gttttcaatt
     1501 tggctccagt cggcatcacc gttcaggtag atttcaccct gctgggtccg tactgtaccg
     1561 gcaagcgtcg agcgcatacc gttaaagttg accgcaagct ggctcggctg catatcaaac
     1621 ggcataaagt tgccgtcgat atccacaccc gtaacctgaa gctgaccaaa caactgcggg
     1681 ctttgcacat caccacccag acgcaagttg gcactcacca tccccgctgc tttttcccca
     1741 cgagtaaaga tggggtttat catcgcaagg ttgaagttac ggatattgac gttgccacca
     1801 agattacggc ggccttgcgg atcggtcacc agcacctgtc catcaaactg gccgttattg
     1861 gtcaggcgga tggtccagcc caattcggca cggttgttac gcaattccgc cgtcagattc
     1921 agtgtctgaa acgccaccgg cagcgccgca tcgttgacgg tttgcgttac ctgcacgtta
     1981 cgccccgaaa gggtgatact gccctgcggc agcccctctt tcgtggtgtc ccaggcaaca
     2041 tccgctttac ccgtgaagat accgctggcc tgagtggttt ctggcataaa cggtttcagc
     2101 atggcgaagt cgaagcggtt gagattcacc accgcacgcc cttcggcccc cgcatcgata
     2161 gtttgcggca cgcacagttc cgcattcggg ttaagccagc agtgtggccc gatgctgatt
     2221 ttttgctcct tattgcggta atccagcgca atatcgcggg tcagcgacca cgggccgacc
     2281 agcgtctgga agcgggtatt gctaagagtt cccttccagc gttcttcttt gcgatcaaaa
     2341 cttcctgcca gattaagctg cccggagaca ggctcgccct gaatccgcaa ctgtagctcg
     2401 tgctgctttt cgctgccttt ggcattcagg gtgacgaggt tgatatttac atccggctgc
     2461 gaaatttgct caacgcgtac gtcgagtttc ccggcgatct gatcggtgga tttaatgtcg
     2521 ccttccacgc gaacctgcgt cacggaaagt tcctgccagc gcaggccgcg cgcggtgata
     2581 tctgccagta gttgtggcgc ttccaccgtg ccgcgtactt tcaccagccc tttcgctgta
     2641 ccgccaagcc ccggcagcgc gttatccagc cccggcgcgt tgatggtggc atcaagattg
     2701 agatctttta cccccagctc gcctttcact tcggcactgt ttggccccag ttccagatga
     2761 agcccaggga tcatccactg catataactg ttgcctttca gcgtgccgtc aacgttcact
     2821 ttgttctgtt tgacgttacc ggtcagcttc agttctggca cctccatctg ccaggtgccg
     2881 ccgtacaggc taccgctggt tttaatcaag ccattgagtt tcgacggcca ctccgggaac
     2941 tctttggcgg tgttaatgcc attaagcgtt agctcaccgc gccaactaat ggcctgctgc
     3001 cagtcgagca acgccttgag ttcagttttc ccttccagcg ccgcgacggt gagtttgtcg
     3061 agattgacct gctgttcatt acctttggca tcaagggtaa tggtggctgg cgggatctcc
     3121 tgtcccttca ctgccgtacg catagagagc gtgtaatcgg tcattttgcc ggtaagtttc
     3181 agtttcagat catccgcctg atactgcttc tcaccagtga acggccagta aagctgtttg
     3241 ctgttcactt ccacgttgag cggcaatccg gcttccgcca gtcgcgtctg ggcgcgtaaa
     3301 tccatatcca ccggaccgga aagattaacg ccaatctcca gctgttcgcg cagcgcgccg
     3361 cccactttta gcttcacttt ttcacctttc aacggctcca cgttcagtgt gctgttgagg
     3421 gtgatatcca ccggccagtt gtctgacagc tgcgccgtgc cgctggcgtt gacgatcccc
     3481 tggttggaat cgatatccag ggcgtccagt ttagtattgc cgtcaatgct gctcactttc
     3541 agcagcatgg tgcgcacggt gatgtccgtg tcgcccgtca cgcgtaactg ctcgccttta
     3601 aactcttcaa tgttcaggtt aagcggcaaa tgcaagtcgg tcatttccgg caataccggg
     3661 cgagaaaaga gatctttcag cgtttcgccg agcggctttt catccggctg cggattttca
     3721 attttcggtt cgaccacttc ttcctgcgcc actttcggca gagcaatcag caggcctttc
     3781 agcgacgtcg gtttcagggt cagggttttc tcctgccagt tcaggccgga ggtgaagtcc
     3841 atcaccgaca ccgtggtgtc atcaatcttg atgttgacgt tgtccagcgc cacccgtgtc
     3901 agggtgatgg gatacggcgt ggagagatcc agcggaccgc tatcttcctc ttcttcaacc
     3961 tgttcagaag gaggcatttt tttactgtcg atgttgacct gaatgtcttt cagcgccagg
     4021 tcattaatac aaacgctact gttccacagg cactcaagac cgaccgccag atgcagatta
     4081 cccgctttta ccgccacgcc tggctgctca taacgaacgt cagacaaggt gagatcgcgc
     4141 cagccgccgg tgaccttgcc aatatccagt cctggcaccc agcgatccgc cgctttaaat
     4201 acaagatgca ggccgctggt ggtgcccacc agaaacgcca ccgatcccag caacagtaag
     4261 ataacgataa ccacgccgag gctgattttt ttccataaac tcataattct ggccccagac
     4321 cgatgtaaaa ctgtaacccg tgttcgtctt tatccgcgac cggtacggca aaatcgagtt
     4381 tgattggccc gaccggcgat tcccagcgca cgccgacccc ggtaccggtt ttaaagtcgc
     4441 tgcggcgaat atcgcttacc gcttcgccac tatcgacaaa caccgcgccc caccattttc
     4501 cggtcacgtt gtactggtat tccagcgatc cggttatcaa cttcgaggcc cctttcaggt
     4561 caccgttggc gtatttcggg gcgatagatt tgtatttgta gccgcgaata ctgcgatcgc
     4621 ccccggcgaa aaaacgcaga tccggcggta ctttgtcgaa atcaccggtt tcaatccagc
     4681 ccagcgtgcc gcgtgtaacg aaacgatggc gatcgtacag tgtgcggatc cagacgttct
     4741 gcgcctggaa aacggagaaa tcgacatctg agccccaggc cgtgttggag tagtcgatag
     4801 agtagcgttg cgagtcgccc caggttggca tcaggccacc acaagaacgc gtgcggctaa
     4861 tcatcacccc aggataaaac agcatcgtgg tgttggtaat ttcaccctga gtaaagtggt
     4921 cgagactcca gcgcaggtta atggcacgct gccaaccgct ggagagatcc cagtagcgag
     4981 aagccaccag cgtagtggag tcagattcgg tatcgttcag gtcagtgcgc ttaaaaccgc
     5041 cctgcaccaa ataatattgt tccagtggat tcttcagcag cggcatttta tagctgaagt
     5101 cgagggtctg ttccggcgcg gaaatactgg tactggtggt caaactgtga ccgtatgagt
     5161 tcatccacgg ctttttccac gtcgctttca cgcgcggtcc cacgtccgta gagtaaccga
     5221 ccccggtttc gatggtgttt tctgttcgcg gcgaaaccac gcccgtcaat ggtaatactt
     5281 tcgtttcgcg cgctttatca aattgtggag ccaccaccac cgagttaaac cagccggtag
     5341 cagaaagtcg gcggttcagt tccgccagat ctttcgattc atactcatcg ccctctttaa
     5401 acggcaccag attttgcagg tattcatcgc ggatttgtga tccttcaaag gtcacatgcc
     5461 caaagcggta acgttcgcca ctgttataat caatatccca gaaggcttta tgcaggccga
     5521 gcgcaatgcc cagctgcgct ttggtaaatt cgctatcgaa ataaccttta cgcaacgcaa
     5581 tactggttaa ggactttttg aaattttcat aatcgccctg gttcagtacc gtaccaatag
     5641 ccgggcgagt atcgagcaat ttcaaatagt ctttatcggt ccgcgcgccg ccgcgcaata
     5701 ccacatcggt gccgccaatt aacaccggca cacctggcgt gactttggcg atcaatacct
     5761 gccgcccttt ctttggcggt ggacggagat caaattcaat ggtcggctgg taataaccca
     5821 gcgctttcag accttcacgg atggcatcat cgacgcgtgc gcgaaagcgg cggtctggcg
     5881 tcacttcatc actttcaatc gtagaaagct gcgcacgaac gttcttttcc agctgtcccg
     5941 ataacccctc gacctgtaga cggacgttcg cggcgacggc agatccgctt aagcagagta
     6001 agcttacaca gcataactgt cggatatagc gcacattttc tcctgaatat ccttttttcc
     6061 tgcccctgga acgccgttaa acggcttaac aaaaatccag taatatggat taaaaagcag
     6121 actaaacccc aaatatttct tatgttttac tttagaccta ttcacggtgg ttattgtgtg
     6181 caaatacgcc tcttgttaca accttaaccc caatgaccga ttttcgggag agcgacacca
     6241 tgagtttatt tgataaaaag catctggttt cccccgccga tgccctgcct ggacgtaaca
     6301 ccccgatgcc cgtagccacg ctgcatgcgg tcaacggtca ctcaatgacc aatgaacctg
     6361 acggaatgga gattgccatt tttgcgatgg gttgtttctg gggtgtggag cgtctgttct
     6421 ggcagttacc cggcgtttac agcaccgccg caggctatac cggcggctat acgccaaatc
     6481 cgacttatcg ggaagtgtgc tccggtgata cgggtcatgc cgaagcggta cgcattgttt
     6541 acgatccttc cgtcatcagc tatgagcagt tgctacaggt attttgggag aatcacgatc
     6601 ccgcccaggg aatgcgtcag ggcaatgacc acggcacgca gtatcgttca gcgatttatc
     6661 cgctgacccc agaacaggat gccgcagctc gcgccagtct ggaacgtttt caggcggcga
     6721 tgcttgccgc cgatgacgat cgtcacatca ccacggaaat cgctaacgcc acaccgtttt
     6781 attatgccga agatgaccac cagcaatatc tgcataaaaa cccatatggt tactgtggaa
     6841 ttggcggaat tggcgtctgt ctgccgccgg aagcatagcg ttacgggtac aaatgtagtt
     6901 tgttgataaa gtgctctttg tctatgcctg atgcggctta aacgacttac caggcctaca
     6961 aaatcgtcca aattcaatat attgcaagga ctgcgtaggc ctgatcggca tagcgcatca
     7021 ggcagtattg catttatcag cagtctgaat tttaaccctc tggcgacttt acagtacctt
     7081 acgctatact agccactgaa aattccggtt cactttcttc gaatcggctt tcaatgtgta
     7141 tttcacacaa attaatcaac tttcccttcc gaggatctgg cctgaaaggt cggataagat
     7201 atgttaaaca gtattttagt catactctgc ttgatcgctg taagtgcgtt cttctcgatg
     7261 tccgagatct cgcttgccgc ctcacgcaaa atcaaactta aactgctggc tgatgaaggc
     7321 aatataaatg cccaacgcgt tctgaatatg caggaaaatc ccggcatgtt ctttaccgtg
     7381 gtccaaatcg gtctgaacgc agtggcgatt ctcggcggta tcgtcggtga tgcggcattt
     7441 tctccagctt ttcacagcct gttctcccgc tatatgtcgg cagagctctc tgagcaactg
     7501 agctttattc tctctttctc gttagtgact ggcatgttta tcctgtttgc ggatttaacc
     7561 ccgaaacgca tcggtatgat tgcgccagaa gcggtggctt tgcgtatcat caacccgatg
     7621 cgcttctgcc tgtacgtttg caccccgctg gtgtggttct tcaacggcct ggcgaacatg
     7681 atcttccgta ttttcaaact gccaatggta cgtaaagatg acatcacttc tgatgacatc
     7741 tacgcggtag tggaagccgg tgcgctggcg ggcgtgttac gtaaacagga acacgagctg
     7801 attgaaaacg tctttgagct ggaatcccgt accgttccgt cttcaatgac accgcgtgaa
     7861 aacgtgattt ggtttgatct ccacgaagat gagcaaagcc tgaagaataa ggtggcggaa
     7921 catccgcact ctaagttcct cgtctgtaat gaagatattg accacatcat cggttatgtc
     7981 gattctaaag acctgctgaa ccgcgtgctg gctaaccaaa gcctggcact gaacagcggc
     8041 gtacaaattc gcaacacgct gattgtgccg gatacgttaa ccctttcaga ggcgttggaa
     8101 agttttaaaa ccgcaggtga agacttcgcg gtgatcatga acgagtacgc gctggtggtg
     8161 gggatcatca ccctcaacga cgtgatgacc acgctgatgg gcgatctggt cggtcagggg
     8221 ctggaagagc agattgtcgc ccgtgatgag aactcatggc tgattgacgg cggcacccca
     8281 attgacgacg tcatgcgcgt gctggatatt gacgagttcc cgcagtcggg caactacgaa
     8341 accatcggcg gctttatgat gtttatgctg cgtaagatcc cgaaacgcac cgattcggtg
     8401 aaattcgccg gctacaaatt tgaagtggtg gatatcgata actaccgcat cgaccagctg
     8461 ctggtgaccc ggatcgacag caaggccacc gccctttcgc caaaactgcc tgacgctaaa
     8521 gataaagaag aaagcgtcgc gtaaccccca gaaacatcaa cggctcctga atcaggagcc
     8581 gtcttattac tgcatagcac tttggttaag ccatctctgt ttgcagacgc ataacctgac
     8641 ggttaacttc ggacatcact gacaaatgca gcttatcctt cacttttggg ataagaatct
     8701 tacccttatc aaattcaaaa gcgccaacgt ccttgatgta taaccgtcca cggaacagga
     8761 tctttacgta cttcgccacc tgaagtgggt tgtaccgttg gaaaattttc attgttatct
     8821 cctgctgagt attacgcctg tgcggtgcca caatcggccc aactattatg agacgcaaat
     8881 tttaatgctt agtgactata gactatccgg gcaatgtttc caccgcgtat aacttttttt
     8941 acctaaaggt tacaattatt cagaattatc tttttaccga agcgcggtct tcagtataag
     9001 cattcatttt tcatatgatt tgtgcgcttg accgcaaact ggcatcacac ttgcgggaaa
     9061 ttcgataaat agcacatatg attaaaactc agatccaagt ggtcggatca cctgcatatc
     9121 ataagaagga aacaccatga ccctacgcaa gattctggca ctcacctgcc tgctgttgcc
     9181 gatgatggct tccgcacatc agttcgaaac cggtcagcga gtgccgccga ttggcattac
     9241 cgatcggggc gagttggtgc ttgataaaga tcagtttagc tacaaaacct ggaacagcgc
     9301 gcagttagtg ggaaaagtgc gagtactgca acatattgct ggtcgcacct ctgcaaaaga
     9361 gaaaaacgcg acgctgattg aagcgattaa atcagcaaag ttaccgcacg atcgttacca
     9421 gaccaccacc attgttaaca ccgacgacgc aattccgggt tccggcatgt ttgtgcgcag
     9481 cagtctggag agtaataaaa agctttatcc ctggtcgcag tttattgtcg atagcaatgg
     9541 cgtcgcgcgc ggtgcctggc agctggatga agaaagttcc gctgtagtgg tgctggataa
     9601 ggatggtcgc gtgcaatggg ccaaag
//
LOCUS       DAJVOY010000158         9577 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVOY010000158 DAJVOY010000000
VERSION     DAJVOY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.158"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            45..728
                     /locus_tag="OQL90_003302"
     CDS             45..728
                     /locus_tag="OQL90_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1369969.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            772..5496
                     /gene="ehaG"
                     /locus_tag="OQL90_003303"
     CDS             772..5496
                     /gene="ehaG"
                     /locus_tag="OQL90_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCS1369970.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENA
                     TAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIA
                     LGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVG
                     NSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTY
                     NLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSK
                     DAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADAL
                     LWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIAN
                     NTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTT
                     GSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTA
                     AHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPT
                     YKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADG
                     EISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALN
                     AIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYT
                     TNKYIADALDGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGA
                     GAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDA
                     TYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVD
                     TGIALGSSSVSSQVIAKGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINV
                     ADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGD
                     KGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQ
                     NSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTN
                     LDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALG
                     TGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSI
                     DYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLS
                     KTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVY
                     KLQGSTNSQGEYSAALGAGIQW"
     gene            5864..7519
                     /gene="lldP"
                     /locus_tag="OQL90_003304"
     CDS             5864..7519
                     /gene="lldP"
                     /locus_tag="OQL90_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1369971.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7519..8295
                     /gene="lldR"
                     /locus_tag="OQL90_003305"
     CDS             7519..8295
                     /gene="lldR"
                     /locus_tag="OQL90_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1369972.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8292..9482
                     /gene="lldD"
                     /locus_tag="OQL90_003306"
     CDS             8292..9482
                     /gene="lldD"
                     /locus_tag="OQL90_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1369973.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         2434 a         2408 c         2474 g         2261 t
ORIGIN      
        1 tctaccggca ttgatcgctg gtcggaggaa ataaggattt ttacatgaac ttgaagaaga
       61 tttttttcag tgctgtcact gtttcagttc tctgcgcatt aacgggatgt gactacattg
      121 aagaaggcaa gccagaaagt agtttattga aacaacaaga agaacataac aataaaattg
      181 atctacttga aaaacaacaa gcacagctaa agagtcaact ggaaactatt caaaaacaac
      241 aaactggcat aataagcagt acaaagactt taactcacgt aattaaatcc gttaaagatc
      301 aacaaaacac ttttattttt accgagttta acccagcaaa aacgaagtat ttcatcctga
      361 acaatggttc ggtggcttta gcgggtcgag tgttatccat tgacgccaca gagaatggca
      421 gtgttattca tatttcactg gtcaacttat taagtacacc tatctcaaat attggtttta
      481 atgcgacatg gggtggtgaa aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat
      541 tacttttcaa cacatcaatg aaatccacat tgaaattatt accaggtcaa tggcaagaca
      601 ttaatttgac cctgaagggt gtctcgccca ataacctggg atatctgaag ttagccatca
      661 acatggaaaa tattcagttc gacaatcttc cctctgctga aaatcggcag aaaagaagca
      721 aaaaataaat gcagttagta cacttataac aaggaatagc gagcatcatt aatgaacaaa
      781 atatttaaag ttatctggaa ccctgcgaca gggaattata ctgttaccag cgaaacggca
      841 aaaagccgtg gcaagaaatc tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt
      901 ggaatgttgt cgtcgtttgg ggcattggcg aatgccggga atgacaacgg tcagggtgtt
      961 gattacggta gtggatcagc tggcgacggc tgggttgcta taggcaaagg ggcgaaagca
     1021 aatactttta tgaacaccag tggttccagt actgctgtgg gttatgacgc tatagctgaa
     1081 ggccaatata gctctgccat cgggtcaaaa acccatgcga ttggtggtgc atcaatggcc
     1141 tttggggtta gtgcaatatc agaaggcgat agaagtatag cactgggtgc ctcttcgtat
     1201 tcattgggcc aatactcaat ggccctcggc cgttattcaa aagcattggg taaattgtct
     1261 attgctatgg gggactcttc caaagcggaa ggagcaaacg ccattgccct ggggcgtagc
     1321 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
     1381 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
     1441 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
     1501 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
     1561 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
     1621 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
     1681 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
     1741 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
     1801 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacaactt
     1861 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1921 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1981 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     2041 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     2101 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     2161 gatgtcgaag ccaacaccgc caatatcgct actaatacca gcaacattgc cacgaatacg
     2221 gcaaatattg ccaccaatac caccaatatc accaacctga cggattccgt tggtgacctt
     2281 caggctgatg ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag
     2341 gataccacca gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat
     2401 gctgttaacg gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat
     2461 atcgccaata acacttccaa tattgccact aacaccacca acatctctaa tctgactgag
     2521 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg
     2581 gcagctcatg gcaccgagac caccagcaaa atcaccaacg ttaaagatgg cgacctgacg
     2641 actggcagca ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg
     2701 acgaatacca ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg
     2761 actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct
     2821 catggcaaca ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc
     2881 agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac
     2941 ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc
     3001 ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc
     3061 acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac
     3121 ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc
     3181 agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg
     3241 ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt
     3301 gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcttgac
     3361 gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga
     3421 aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc
     3481 agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg
     3541 gatggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg
     3601 aacgccgagt acaacaacgt cggtgacgct ctggatgcgc ttgatgataa cgccctgctg
     3661 tgggatgaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc
     3721 agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac
     3781 ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag
     3841 ctcgctggta acaccgacgc aacctatatc caagaaaacg gtgcgggtat taactatgtg
     3901 cgtactaacg acgacggctt agcgttcaac gacgccagcg cacagggtgt tggcgctaca
     3961 gctataggtt ataactctgt cgccaaaggc gatagcagcg tagctattgg tcagggcagc
     4021 tacagcgacg ttgatacggg tatcgcccta ggtagcagct ctgtttccag ccaagtgatt
     4081 gccaaaggct cccgtgacac cagcataacg gaaaatggcg ttgttattgg ttacgacacc
     4141 acggatggcg aactgctcgg tgcattgtct atcggtgatg acggtaaata tcgtcaaatc
     4201 atcaacgtag ccgatggttc cgaagcccat gacgccgtta cggttcgtca attgcagaat
     4261 gcgattggtg cggtcgcaac cacgccgact aaatacttcc acgctaattc aacggaagaa
     4321 gattcactgg cagtgggaac tgactcgctg gcaatgggtg cgaaaaccat cgtgaatggc
     4381 gataaaggta ttggtatcgg ttatggtgcc tacgtggacg cgaatgcact taacggcatt
     4441 gccattggta gcaatgcgca agtcattcat gtcaacagta ttgcgatagg taatggttct
     4501 acgaccactc gtggcgctca aaccaattat accgcctaca acatggacgc accgcagaac
     4561 tctgtcggtg aattctcagt cggtagtgcg gatggtcaac gtcagatcac aaacgtcgca
     4621 gctggttcag cggataccga tgcggttaac gtgggtcagt tgaaagtcac tgatgagcgc
     4681 gtagcgcaaa atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact
     4741 cgcgttacta atatcgaaaa cggtattggc gacattgtca ccaccggtag caccaagtac
     4801 ttcaagacca acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt
     4861 ggttctggtt ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca
     4921 gaggaagaaa atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta
     4981 gctgccggtg ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca
     5041 ggcggcgtgc gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc
     5101 ggtggtggca acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac
     5161 gacgcggtga actacgcgca gttgaagcaa agcgtgcagg aaacgaagca atacaccgat
     5221 cagcggatgg ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt
     5281 atcgcttctg caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg
     5341 gcttctattg gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg
     5401 gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa
     5461 tactccgccg cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta
     5521 agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgctccggg
     5581 tcaacaatct aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc
     5641 cccacaagaa ttggccctac caattcttcg cttatctgac ctctggttca caattcccca
     5701 attaaaactc acatcaatat tgccaataca caacatttag ttaaccattc attgtcatta
     5761 tccctacaca acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca
     5821 acagactcat tacacgatgt gcgtggactc caggagacct gcaatgaatc tctggcaaca
     5881 aaactacgat cccgccggga atatctggct ttccagtctg atagcatcgc ttcccatcct
     5941 gtttttcttc tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac
     6001 ggtagcaatc gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct
     6061 ggcctcggtg gtttatggct tcttctatgg tttgtggccc atcgcgtgga tcattattgc
     6121 agcagtgttc gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc
     6181 tattctttcg ataacccctg accagcgtct gcaaatgctg atcgtcggtt tctgtttcgg
     6241 cgctttcctt gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct
     6301 ggtcggcctg ggttttaaac cgctgtacgc cgccggactg tgcctgattg ttaacaccgc
     6361 gccagtggca tttggcgcga tgggcattcc aatcctggtt gccggacaag taacaggcat
     6421 cgacagtttt gagattggcc agatggtggg gcgtcagcta ccgtttatga ccattatcgt
     6481 gctgttctgg atcatggcga ttatggacgg ctggcgcggt atcaaagaga cgtggcctgc
     6541 ggtcgtggtt gcgggcggct cgtttgccat cgctcagtac cttagctcca acttcattgg
     6601 gccggagctg ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct
     6661 caaacgctgg caaccagttc gcgtattccg cttcggtgac ttgggggcgt cacaggttga
     6721 tatgacgctg gcccacaccg gttacaccgc gggtcaggtg ttacgtgcct ggacaccgtt
     6781 cctgttcctg accgctaccg taacactgtg gagcatcccg ccgtttaaag ccctgttcgc
     6841 atcgggtggt gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcgataaact
     6901 ggttgcccgt atgccaccag tggtcagcga ggctacagcc tatgccgccg tgtttaagtt
     6961 tgactggttc tctgccaccg gcaccgccat tctgtttgct gcactgctct cgattgtctg
     7021 gctgaagatg aaaccgtctg acgctatcag caccttcggc agcacgctga aagaactggc
     7081 tctacccatc tactccattg gcatggtgct ggcgttcgcc tttatctcga actattccgg
     7141 actgtcatca acactggcac tggcactggc gcacaccggt catgcattca ccttcttctc
     7201 gccgttcctc ggctggctgg gggtattcct gaccgggtcg gatacctcat ctaacgccct
     7261 gttcgccgcg ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt
     7321 tgccgccaat accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat
     7381 cgcctgtgcg gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa
     7441 acacagcctg atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt
     7501 aacgtggatg attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat
     7561 cgtgtgcggg cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgag
     7621 cgccaactgg cgatgcaact cggcgtatca cgtaattcac tacgcgaggc gctggcaaaa
     7681 ctagtgagcg aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt
     7741 catgacacat ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat
     7801 ccggattaca gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg
     7861 catgcggcaa tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca
     7921 acgctaagtg aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt
     7981 gccgaagcct cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg
     8041 caatcctcag tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa
     8101 ctgaccgaac aacatcaggc tgtcattgac gccatttttg ctggtgatgc cgacggggcg
     8161 cgtaaagcaa tgatggcgca ccttagtttt gttcacacca ccatgaaacg attcgatgaa
     8221 gatcaggctc gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg
     8281 gagaaaaacg catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc
     8341 tgccgccgtt cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc
     8401 gcaacgtgga agatttgtca gaagtggcac tgcgccagcg tattctgaaa aacatgtccg
     8461 acttaagcct ggaaacgacg ctgtttaatg agaaattgtc gatgccggta gcactggcac
     8521 cggtgggttt gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg
     8581 acacgcatgg cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg
     8641 cgccagccat caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta
     8701 tgcgtaacgc gctggagcga gcaaaagcag cgggttgttc gacgctggtt ttcaccgtgg
     8761 atatgccgac accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccaaatg
     8821 cggcaatgcg ccgctacttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc
     8881 tgaacggtcg tccacatgat ttaggtaata tctcagctta tctcggcaaa ccaaccggac
     8941 tggaagatta catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc
     9001 ttgagtggat ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg
     9061 aagatgcgcg cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg
     9121 gccgccagct ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt gcagatgcgg
     9181 tgaaaggtga tatcgccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc
     9241 gtatgattgc gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg
     9301 caacagcggg ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag
     9361 tggcgatgac gctgactggc gcgaaatcga tcagtgaaat tacgcaagat tcgctggtgc
     9421 aagggctggg taaagagttg cctgcggcac tggctcccat ggcgaaaggg aatgcggcat
     9481 agtcgtttgc gcccccgtta ctttattcgt accccttata atggggtgtt agccagccag
     9541 acccggcatg attactgccc ccagtcgtcc atgatcc
//
LOCUS       DAJVOY010000159         9489 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVOY010000159 DAJVOY010000000
VERSION     DAJVOY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.159"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..181
                     /locus_tag="OQL90_003307"
     CDS             <1..181
                     /locus_tag="OQL90_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1369974.1"
                     /translation="MMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLAT
                     LPSGDKVAAAVGITP"
     gene            complement(286..822)
                     /gene="rcdA"
                     /locus_tag="OQL90_003308"
     CDS             complement(286..822)
                     /gene="rcdA"
                     /locus_tag="OQL90_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1369975.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            906..2114
                     /locus_tag="OQL90_003309"
     CDS             906..2114
                     /locus_tag="OQL90_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1369976.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            2114..2929
                     /gene="ybjI"
                     /locus_tag="OQL90_003310"
     CDS             2114..2929
                     /gene="ybjI"
                     /locus_tag="OQL90_003310"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1369977.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            3015..3299
                     /locus_tag="OQL90_003311"
     CDS             3015..3299
                     /locus_tag="OQL90_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1369978.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3340..4570)
                     /gene="mdfA"
                     /locus_tag="OQL90_003312"
                     /pseudo
     CDS             complement(3340..4570)
                     /gene="mdfA"
                     /locus_tag="OQL90_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4855..5451
                     /gene="ybjG"
                     /locus_tag="OQL90_003313"
     CDS             4855..5451
                     /gene="ybjG"
                     /locus_tag="OQL90_003313"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1369979.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5509..6267
                     /gene="deoR"
                     /locus_tag="OQL90_003314"
     CDS             5509..6267
                     /gene="deoR"
                     /locus_tag="OQL90_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1369980.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6314..7516)
                     /gene="dacC"
                     /locus_tag="OQL90_003315"
     CDS             complement(6314..7516)
                     /gene="dacC"
                     /locus_tag="OQL90_003315"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1369981.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKDVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7763..8388
                     /gene="gstB"
                     /locus_tag="OQL90_003316"
                     /pseudo
     CDS             7763..8388
                     /gene="gstB"
                     /locus_tag="OQL90_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(8385..>9489)
                     /gene="yliI"
                     /locus_tag="OQL90_003317"
     CDS             complement(8385..>9489)
                     /gene="yliI"
                     /locus_tag="OQL90_003317"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCS1369982.1"
                     /translation="SFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLPDNHG
                     MLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWLSYSE
                     VGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYLFIAL
                     GENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQGMAMN
                     PWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGTEQP
                     VFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRILTDR
                     GQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2299 a         2451 c         2340 g         2399 t
ORIGIN      
        1 catgatgtcg ggtggaaaca ttctggcctt acaaaaaatt ctcggacacc acgatataaa
       61 aatgactatg cgttacgcac atctggcacc ggatcatctg gaaacggcgc tccgtttcaa
      121 tcctctggca acgctgccaa gtggcgacaa agtggcggca gcggttggca ttaccccgta
      181 ataaccacca ctgaccacca acctaactta ttgtttttaa tctaacttat tgttttcatt
      241 aacccgttta cataaatggg ttttttgtta tctaaaattt atctactacc ctgcaaccct
      301 ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga caaaatgcag
      361 cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa accattgttc
      421 gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata gcggttttcg
      481 gctagccagc gcgtagagct ggtacatcag ctccatatta tccggcgttg caacctgtga
      541 gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa catcgctaaa
      601 aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct ccagcaacaa
      661 ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg caagggtagc
      721 aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca gcgtggcctg
      781 gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc ctctgtctgc
      841 aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt attttgacgt
      901 gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg gcgctgttta
      961 tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc aacccgtacg cctgctatcc
     1021 gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt ctgtcgatcg
     1081 gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg acacgtaatg
     1141 tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt ctggcactct
     1201 ggctgacatc gcccctgctc tttgccgttg gtctcggcgt ctttggggca agttttggtt
     1261 ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga gcgagaaatg aataaaacgg
     1321 ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct ggtgtcggga
     1381 tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg gcgctggtag
     1441 gtatcgcgcc tatttatatc gccattcagg caatccctga cggtacgggc aaaaatgctg
     1501 ccgatggcac ccagcatggc gaaaaaggca ttccttttta tcgcgatatc cagttgctgt
     1561 tgattggtgt tgtagtgctg gcgatggcct ttgccgaagg ttctgccaac gactggttac
     1621 ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg atttatgccg
     1681 gttttaccct ggggatgacc gttggacgct tcactggcgg ttggttcatc gaccgttaca
     1741 gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc gttgggtatt gggctgatta
     1801 tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga ctgggtgcct
     1861 cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat gcaccaaccc
     1921 gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg ccgctgctgg
     1981 gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg gcgctggtta
     2041 ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg cagacggcga
     2101 tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta ctttcttaag
     2161 cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa tgaaagcaca
     2221 aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct ctttcttccc
     2281 tgaaattgct aatgaaattg cctttgtggc tgaaaacggc ggctgggtag tgagtgaagg
     2341 caaagatgtt tttaatggcg agctgtcgaa ggatgccttt actactgtcg tggaacattt
     2401 gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct atacgctcaa
     2461 aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc tggaatacgt
     2521 tgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc tttccgatga
     2581 actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta tggtgccagt
     2641 ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag ccaatggcct
     2701 tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct ttggcgatgg
     2761 cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg aaaatgccgg
     2821 cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg aaggcgtact
     2881 ggatgtgatc gataaagtgc ttaaacacga agcgccattt gaccaataaa aacggcagca
     2941 cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta acccgctgca
     3001 ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt ttaatgggct
     3061 ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga tacaaagtgg
     3121 ttgtagttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact gtgccgcaaa
     3181 ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat gctcgccatc
     3241 ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat caccattaat
     3301 tcgagaatgc ctgatcgcac aaagcagtca ggcatttttt tacccttcgt gagaatttcc
     3361 catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc cgttgacaag
     3421 gttgaagaga ttaaactgtc cgttgccccc gttcagccag gcatgtttgc tgatttcaat
     3481 accaacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg tacctttact
     3541 catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac caataccgaa
     3601 agcataaata cttaacccgg cagtcatcca taaatacgcg tgcgatgaga taaccgttgc
     3661 cgcagcagcg accaacagac caatcataat cggccagccg cccataatta tcagcgaacg
     3721 tacggtgcgg cacgaggtca gacgcgctaa cagcaagtta cccgcaatta acgccccgaa
     3781 aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg taatgatgat
     3841 aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca gcgccagcgc
     3901 ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga gttctttcag
     3961 tgacagtttc tcgcctatac gcgtggcggt ttcaggcata gctcgttgca gaccgaaaaa
     4021 ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca gcacatggat
     4081 ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt tcgccatcag
     4141 cgcggtgatc ttgatacaaa ccgcctcttc gaaagattcc tgaattgcgg cgtatcccac
     4201 agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagagtga attgttcaat
     4261 gttttgcgcc agtaatattg ccagacaggt gatgataaac cacaccaccc ccgccagcat
     4321 caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta aaaacatccc
     4381 gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct gatattgttc
     4441 caccacggcc aacataccgg gttgaatcat atctgccgat ataggttgaa aattcgtaaa
     4501 gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg gaagctaatt
     4561 tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa aagcgcagct
     4621 aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac attattgcga
     4681 gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc acccaccgta
     4741 aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc tgcactttat
     4801 tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga agttatgctg
     4861 gaaaatttga atctctctct attctctctt attaacgcga cgccagactc ggctccgtgg
     4921 atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc gctgctggcc
     4981 gcggtgcttt ggttgtgggg gcttacagca caacggcaac tggtgataaa aatcgctatc
     5041 gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc gcacgaccga
     5101 ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga ctcattccca
     5161 agcgaccacg gtacggtgat tttcaccttt gcactggcat ttttatgctg gcatcgcctg
     5221 tggtccggct cacttttaat ggtgctggcc gtcgtcattg cctggtcgcg cgtttatctt
     5281 ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat tggctgcctt
     5341 agtgcccaga ttatctggca agcgatgggg cataaactct atcaacgtct gcaatcgtgg
     5401 tatcgcttct gttttgcatt accgatccgc aaaggctggg tgcgtgactg atctctgcta
     5461 aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat ggaaacacgt
     5521 cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa gttacatctt
     5581 aaagacgccg ccgccctgct tggggtttcg gagatgacga ttcgtcgcga tctgaacaac
     5641 cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgca tagtgccagc
     5701 cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg ggcggcaaaa
     5761 ctggctgcga cactggtaga acccgatcag accctctttt ttgactgtgg caccaccacg
     5821 ccgtggatta ttgaagcgat tgataatgaa atccctttta ccgccgtttg ttattcgcta
     5881 aatacctttc tggcgctgaa agagaaaccc cattgccgcg catttctgtg cggtggtgaa
     5941 tttcacgcca gcaacgccat tttcaaaccc atcgattttc agcaaacgtt gaataatttt
     6001 tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg cgctacctgt
     6061 tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca aaagcatgtg
     6121 ctggttgtcg accacagtaa atttggcaag gtccgtccgg cgcgcatggg tgacctgaaa
     6181 cgctttgata ttgtggtgag tgattgttgc ccggaagatg agtatgtgaa gtacgcgcag
     6241 acgcaccgta ttaagttgat gtattaatga cgtataaccg gatgacgttt cgcgccatcc
     6301 ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc atcatcacga
     6361 aatcccacat ccgaccaaag aatccgccct cttccacatt ttccatcacg atcagcggac
     6421 gctgctcaat ggatttaccg ttaagctgga aatcaatggt cccgacaacc tgaccttttt
     6481 tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg tttttcagct
     6541 gcccacgcgg tatggtcact gagcccgctt cgcctgcccc gagattcact tcgctcttat
     6601 caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc gtcacggttt
     6661 caaagaagcg gaaaccccag gtcaataatt tctcagactc attaaaacgg atacggtcag
     6721 ttttcgcccc caacactacg gagattaaac gcatatcgcc ctgggtagcc gaagcaacca
     6781 gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc atcaacattc agattgctgc
     6841 tccacagcag acggttacgg ttaggctgac gaattttgtt gaaggtgaac tctttctctt
     6901 tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc tttacccagc aatgccatat
     6961 cgcgcgcggt actgaactgc cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt
     7021 tggtcagacc cagttttttg gcataaccat tcatcagacc aataaatgac tcctggctcc
     7081 cggcaacgta atcggccagc gcaatacagg cgtcattacc ggactggata atcacatctt
     7141 tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag gaacattacc gatgaaccac
     7201 gcagtgccgg atttcccgtc gcccaggcat ctttaccgac cgtcaccata tcggtgagtt
     7261 taatcttatc ggccttaagc gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc
     7321 tcgcgggatc cagtttctca tccgcattgc cttctgccag cactttaccg ctggcgtaat
     7381 ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc aacggtttgt tcggccgcga
     7441 atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc tgcaagacca cgaaggagag
     7501 aggagtattg cgtcataaat gccacccgag tatccattca aaaaaaatac gttatatcgc
     7561 cgtatgataa gttcggtgat taataacgca cacacaaact taaagaaacc gtaagttggt
     7621 aaagttttta aagtttatgc aatatcctgc cgcttcgctg cttctggtgt catttttttg
     7681 ccagctgttg cttaaaagtt aactttactt caccatcgga gtcctgttca gcctgtggct
     7741 gcctgtcgga aaaggggtga gtatgattac gctgtggggt cggaataatt caaccaacgt
     7801 aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat tctcgcgggc
     7861 cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa cgggctggtg
     7921 ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc cattgtccgc
     7981 tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg
     8041 gaagcggaaa aatggatgga ctgggcaaac cagacgctca gtaatgccca tcgagggatc
     8101 ctgatgggat tagtcagaac accaccggaa gagcgcgatc aggccgccat tgatgccagt
     8161 tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg
     8221 ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat ctacaatttg
     8281 tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta tcagcaactc
     8341 actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt gcgtgggcta
     8401 actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca
     8461 gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc ttctgtcact
     8521 ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc aataaataat
     8581 ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct cacagcgggc
     8641 gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc tttcgcttcc
     8701 ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc gtagtttttg
     8761 cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt cagccacagt
     8821 gcattactcc acggattcat cgccattcct tgcggattac gaatgccata agaccagatc
     8881 tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc gccctgatcg
     8941 gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg gcgctgattg
     9001 ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg cccgccaaaa
     9061 tggttgccgg tagacagttt tggcatctgg cggaagacgg tgcggaagtc ggtcactttt
     9121 gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc atcatcgcca
     9181 acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc taaaaccacg
     9241 tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat
     9301 aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat taacataccg
     9361 tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg cagtacttcg
     9421 acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag gggcacaagg
     9481 aaaaaggat
//
LOCUS       DAJVOY010000160         9384 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVOY010000160 DAJVOY010000000
VERSION     DAJVOY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.160"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(313..891)
                     /gene="mocA"
                     /locus_tag="OQL90_003318"
     CDS             complement(313..891)
                     /gene="mocA"
                     /locus_tag="OQL90_003318"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCS1369983.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            994..1764
                     /gene="yqeC"
                     /locus_tag="OQL90_003319"
     CDS             994..1764
                     /gene="yqeC"
                     /locus_tag="OQL90_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCS1369984.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1812..3437
                     /locus_tag="OQL90_003320"
     CDS             1812..3437
                     /locus_tag="OQL90_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCS1369985.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHINRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OQL90_003321"
                     /pseudo
     CDS             complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OQL90_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OQL90_003322"
     CDS             complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OQL90_003322"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCS1369986.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5896..7107)
                     /locus_tag="OQL90_003323"
     CDS             complement(5896..7107)
                     /locus_tag="OQL90_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCS1369987.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7165..8361)
                     /gene="dpaL"
                     /locus_tag="OQL90_003324"
     CDS             complement(7165..8361)
                     /gene="dpaL"
                     /locus_tag="OQL90_003324"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCS1369988.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8419..>9384)
                     /gene="ygeW"
                     /locus_tag="OQL90_003325"
     CDS             complement(8419..>9384)
                     /gene="ygeW"
                     /locus_tag="OQL90_003325"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCS1369989.1"
                     /translation="FSYASALNLLGLAQQDLDEGKSQIAHGETVRETANMISFCADAI
                     GIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQCDIDHPTQSMADLAWLR
                     EHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFGMDVTLAHPEGYDLIPD
                     VVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKVMEERTELLRANDHEGL
                     KALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADISGVSCKEGEVTEGVFE
                     KYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQERVK"
BASE COUNT         2194 a         2290 c         2455 g         2445 t
ORIGIN      
        1 cttagcttat acagatatca ttgagtgacc ggtgttgaga aatccggtgg tttaggggga
       61 tagagcaaca atcgcaccac tcttgccagg tttggcggga aatataaaaa aatgtgaggc
      121 ggctcgaaac ttaagggctt tttgtgaatg aactcacagg tgctaaatga gaattaaaat
      181 cactcctgaa aattattatt gccagcccat atatcacttt ttctcttttc tcaaaatcag
      241 tgattcctta tcattatgat aaagagtgct ggagggatta aatatttgaa cgcaatcgtt
      301 taactacgtt ttttaaattt tagtatacct tttttgcact gtaataaagt cgtccggagt
      361 atcaatatct aaaattattt ctgcattttc tacttcgacg gaataatgag ctcccataag
      421 gagtgcctga cgcatattgg tgatattggg gcgcttgatt gcctgcatca ggcatggttt
      481 tgacactaaa atcggatggc cggggatgcc attatggagt ggcagtattg cgccatcatt
      541 tcgtaacgac cagatttttc tgaaaatatc gatggtgagg gttggcatgt caccgtgggt
      601 gagaaaacaa tgttctgttt gcactgcggg tactgcggcc tttactgatg ttagtaaacc
      661 ctgcgcataa tctgggttgt ggataatagt gatattgctc tggttcacat aacgttcgtg
      721 cagttcatta ccacgatagc cggtgactaa aataattcgg ctacaaaact gcaacgcatt
      781 tttgatactt gtatcaagaa ttgtcccctg ttgccagggt aacatcattt tccattgccc
      841 cattcttgat gataatccgg cagcggtaat tatacagtcg atggctgaca tagtggttcc
      901 attatttata ggggctaata acgcctgata attgcgttac ttcctgatgg attaagttgg
      961 ggataattta tcccagagag gtcataaaga ctcgtgaaaa gtatagttga cccatcggca
     1021 ttattcattg atttaggtgc gcagaaacgt cccactgtga tttctgttgt tggtgctggg
     1081 gggaaaacca gcttgctttt ttggctggca gaactgctcc aggctagcgg caggcgtgtg
     1141 ttaatcacga cgacaacaca tatgtttatg ccaacgtctc actggcccgt ggttttctgt
     1201 cgtgatcccg ccatgcttcc tcatgcgtct cttacatccc ccatttcatt ttgttttcac
     1261 agctggaaag cgaaccaggg aaaagtgcag ggatttacgc cagaagcgat tgatgcactg
     1321 gtgcaacgac cagagtgtga cgtaattctc attgaagcag atggctcgcg tgcaatgccg
     1381 ttaaaagcgc ctgatgagca cgaaccttgc atacccaaaa gcagttgctg cgtgattgct
     1441 gtgatgggag gacatatttt gggcgcgaaa gtgagcacag aaaatgtcca tcgctggtcg
     1501 cagtttgctg atattactgg gttaacacct gatgcaacct tgcaactgag cgatctcgtt
     1561 gcgctggttc gccaccctca gggggcgttt aaaaacgtac cgcaaggttg tcggcgagtc
     1621 tggttcatta accgtttttc tcaatgtgag aatgcgattg cacaaagcga gctccttcaa
     1681 ccgctgcaac aacacgacgt agaggcaatc tggctaggcg atatacaaga acatcctgca
     1741 atcgcgcgca gatttgtgaa ttagcggccc gggtattccg ggttactgaa aggatatcca
     1801 ttgaggttgg tatgaatatt ttcacagagg ctgcaaaact cgaagagcaa aattgtccgt
     1861 ttgcgatggc gcaaattgtt gatagccgag gctcgactcc ccgccattct gcacaaatgt
     1921 tagtgcgcgc cgatggttct atcgtcggta caattggtgg cggaatggtt gagcggaagg
     1981 tgattgaaga gtcacttcag gcattgcagg aacgtaagcc gcgattattc catggacgta
     2041 tggctcgtaa cggtgcggat gctgtcgggt cagattgtgg aggtgcaatg tcagtattta
     2101 tcagcgtcca tggtatgcgt ccacgtctgg tgttgattgg cgcggggcat atcaaccggg
     2161 cgatagccca gagtgccgcg ctattaggat ttgatattgc cgttgccgat atttatcgcg
     2221 aaagcctcaa tcctgaacta ttcccaccat caaccacgct tctccatgct gagtcgtttg
     2281 gtgcggcagt ggaagcactg gatattcgcc ctgataattt tgtcctgatt gccacgaata
     2341 atcaggatcg tgaagccctc gacaaactca ttgaacagcc cattgcatgg ttggggttgc
     2401 tggcaagtcg ccgcaaggtt cagcttttcc tgcgtcaatt gcgtgagaaa ggcgtggctg
     2461 aagaacatat tgcccgttta catgcgcccg ttggttacaa cataggtgcg gaaacgccgc
     2521 aggagatcgc catcagcgtg ctggcagaaa tattacaggt gaaaaataac gcgccgggtg
     2581 ggctgatgat gaaaccttct catccttccg gacaccagct ggtggtgatt cgcggtgcgg
     2641 gagatatcgc cagtggtgtg gcgctacgtc tgtatcatgc gggttttaaa gtgatcatgc
     2701 tggaagtgga aaaaccgaca gtgattcgtt gtaccgtggc gtttgcccag gccgtgttcg
     2761 atggcgaaat gacggtcgaa ggcgtcactg ctcgcctggc aaccagctct gcggaagcga
     2821 tgaaacttac cgaacgcgga ttcatccctg tgatggtaga tcccgcctgt tcattgcttg
     2881 atgaactgaa accgctttgc gtggtggacg ctattctggc gaaacagaat ttgggaacgc
     2941 gggcagatat ggcaccagta acaatcgcgc ttgggccggg ctttactgca gggaaggatt
     3001 gtcatgcggt aattgaaaca aatcgcgggc actggctcgg tcaggtgatt tactctggtt
     3061 gtgcgcagga gaataccggt gttcctggca atattatggg gcataccacc cgacgggtga
     3121 tccgtgctcc tgctgcaggc attatgcgat ccaacgtgaa attaggcgat ctggtgaaag
     3181 agggcgatgt gattgcctgg attggtgagc atgaaattaa agcaccgttg acggggatgg
     3241 tgcgtggctt gttgaacgac ggcctggcag tggttggtgg ttttaaaatt ggtgatatcg
     3301 atcctcgtgg tgaaaccgct gatttcacca gcgtttctga taaagcccgg gcgattggcg
     3361 gcggcgtact tgaggcgtta atgatgttga tgcatcaggg cgtgaaagcg acaaaagaag
     3421 tgctggaagt ggcttaaatg aaaatggccg gatggtatta cccacccggc cttaaaatta
     3481 ctgacaaatc accgttccgg ttttgccttc aatcccttct ttcgctttgc tcaatacggt
     3541 aatcagtgct tcacgacctg cgcgagagcg agcaaacgag gcggcagctt ccactttagg
     3601 cagcatagaa cctttcgcaa aatgaccttc ttcgataaag cgttcagcat cacttaatga
     3661 caggcgatcg agccactgtt cattctcttt accgaagtta atggccactt tttctaccgc
     3721 agtcagaatg atcagcatat cggcatcaat catttctgct aaacgggcgc tggcccagtc
     3781 tttatcgata accgcgctgg caccacgcag atggttacct tcacgaataa cagggatacc
     3841 accaccgcca acggtgatca ccacctggcc tgcatctacc agagctttaa cagtttcttt
     3901 ttcaatgata tcaaccggtt ttggcgaggc aacaacacgg cgatagccgc gaccagcatc
     3961 ttctttcaga gtgtaaccct gttttgtcag ttgctctgct tcttgctcgg taaagaacga
     4021 accgatcggc ttggtcgggt tgaggaatgc cggatcgtta gcgtcgactt cgacctgagt
     4081 aaccagcgtt gctacaggtt tattaatgcc ccgagaaagc agttcttctc ttaacacgtt
     4141 ttgtagatcg taaccaatat aaccctagct taaggcaaca cagacagaca tcggcagcat
     4201 tggcgagtgt gcttcagttt ttgcggcagc ttcaaaggcc tggttaatca tgcccacctg
     4261 cggcccgtta ccatgagtga cgataacttc atgtccttgg gcaattaaat caacaattgc
     4321 ctgagaagta atttttaccg ctttcatttg tccggccagg tcatcgccca gcgcattccc
     4381 gcccagggcg agaacaattt tcttactcat ttttcctcac tcatcattct tgattgtgta
     4441 tagatccctg acagtggtta gagcacggga gggacaaacg gtttgcgtcg caggaaacga
     4501 cctcgcccgg ctttgcctgt aaaggtgccg tcacagaaaa tcgtttcacc acgggataag
     4561 gttctgacaa tcgcgccctg acaggtaaaa ccctcccatg gcgagtagtc ggcgttgtcg
     4621 tggagatggc gatgctgaat ttgttggctc tgacgtgggt cgataatcac cacgtcgcca
     4681 tcggaaccgg gcgctaataa tcctttttgc ggccacaggc caaacagcct ggcgggcatt
     4741 gcgctggtta attcaacaaa gcgttccggt gttatacgtc ccgtcatcac gccactggaa
     4801 aacagtaact gcatgcggtt ttccacaccg ggtaagccat ttgggcagcg actgaaatcg
     4861 cctttagaaa tttgcaggcg ttgagccatc gagaaggtgc agtgatcggt cgccaccacg
     4921 tcaatcgcac catcgctgat gccacaccac agtttgtcct gctcgcgtac gttacgcagc
     4981 ggtgggctaa gaatgaactt catgccatct tctgtatcgt aactgcgttc gtccaacagg
     5041 agatattgtg ggcaggtttc aacccagact ggctggtgat tcgcacgggc aagacgcaga
     5101 taatccagac ctaagccgtt agacaggtgc acgatataga gcggggcgtt accggcaatt
     5161 tgtgccaggt taatcatgcg ggcgatggct tccgcttcgc attccagagg gcgactcaag
     5221 gcatgatagc gcggcgcggt taaccctgcg gcgataaatt ccgcccgctt gctggcgata
     5281 gccgcatcat tttccgggtg cacggtggtc agcgcgccgg attcatgcaa gcggcgtaat
     5341 gcctgcaaaa cctcgtcatc gttgagtttg tattgatagg ttaaatagag tttaaaactg
     5401 ctcagtcctt cctcgaccat catcgggatt tcgtcgagga ttgcgtgatt aatgtgctgg
     5461 atcacaccgt gaaagctgta atcgatgacc gctttatggg cggcataacc acgataaacc
     5521 tccagttgat ggcgtaaccg acagccgttt gggccaaatc ccatatggtc aataatggtt
     5581 gttgtaccgc cacacgcagc tgcgcgggta ccggtaaaaa aatcatcaca actgcgcgcg
     5641 atgccgacat caatattgaa atgcgtatgg acatccacgc cgcctgggaa aacgtaacag
     5701 ccagtggcat caatttcttc atacgggagc tgcggcgaaa tattgttgcc caactggcga
     5761 acaatcccgc tttcaatcag caaatcctgt ttggcttgtc catctgcgtt aacgacagtg
     5821 ccgtttttga tcaatacgcg catagcaaac tccaaaaacc ctccggcgaa ccggagggga
     5881 ataagtgaag agtaattact ctgttgccag ccagcttaac gggattgcag cgtacatcgc
     5941 ggcacaggtc accaggtgag atttccaggt tttttcgtta ggtgcatgcg cttcaggttc
     6001 tttacccggg ccaaagccga tcaccggaat gccgtgacgg cccatgatag atacgccgtt
     6061 agttgagaag gtccacttat caacaaccgg cgctttgcca aacagccctt cgtaggcatt
     6121 caccagtgct ttaacggtga agtgatcttc ttccactttc caggtcggga agtagcattc
     6181 ggttgggtaa accaggccag tccaggacgg acggtcgtag ttgtacatag aaacaaccgc
     6241 gttagctttc tgtactgcag gcagggcgcg gatttcgtcc agcgcgcctt cccaggtttc
     6301 gccccaggtc agacggcggt caatagagac ggcgcagcta tctgctacag cgcaacggct
     6361 tggggatgtg aagaagattt cagaaacggt gagggtgcct ttgccgagga attcgtcata
     6421 acccagacgt tgggagagtt cttgtaattc gccaagaatc ggacccattt tgaaaatggc
     6481 gttgtcaccg cgttctggcg cagaaccgtg gcagctaaca ccctgaacat caatacgaat
     6541 ttccatacga ccgcgctgac cacggtatat ctggcagtcg gttggttcgg tactgaccac
     6601 aaattccggg cgaatgccgg attgttcaat aatgtactgc cagcacagac cgtcgcagtc
     6661 ttcttcctgc acagtaccgg taaccagcag ggtatattca tcttccagac cgaggtcttt
     6721 aatgatttta ccggcataaa ccatagatgc catgccgcct tcctggtcgg aagtaccgcg
     6781 accaccgatt agctcatcag tttccatgcc ttcgtacgga tcgaagtccc agtttttgat
     6841 gttgccaatg ccgacggtat cgatatgagc gtccattgcc accagacgcg ggccgtggcc
     6901 gatataaccg agaacgttgc ccatcgggtc gatttcaact ttatcgaagc cgactttttc
     6961 catctcttct ttaatacgat gtactacgcg tttttcgtcg cagctttcac tgggaatagc
     7021 aaccatgtcg cgcaggaagc gagtcatgtc cgcctggtaa tcttttgctt tttcaagaat
     7081 cagtttgaat ggaatattct tagccattat ttttctccag attttttact ccccgttttg
     7141 cagggagtag ttccaatagg gtgattaagg tgctacagcg tgtttgcctt cccagacaac
     7201 ttcgcggtag tgcttcacgt cggtgtcgcc ttcagtactg ataaccagca ctacggcatc
     7261 tttgttcagc gccagttttt ccatcaggct ttgacgttgc gggtgataat gaaccgctgc
     7321 gagaacgccc aaaccgacag cgccggattc accggagatg atgcgcgggt cgttgccgta
     7381 cggattaccc agcacgcgca tacctaatgc ggcaacgctg tcctggcagg agatgaattg
     7441 ggtggcgcag ttacgtagga tttcccagcc cagcgggtta ggttcgccac aggccaggcc
     7501 cgccatgatg gtggccatat caccgccaac gttgacgatg tcgcctttga cgccggagcg
     7561 ataaatacag tcagctttgt caggttcaac aataatgctg tgcagatttt gcgggctgta
     7621 gacgtcgacc agataaccca gcacaccacc ggccattgct ccgacaccgg cttgcagcag
     7681 aacgtgcgtc ggggttacgc ccatttcacg catttgctcg acggcttcat ctgccagggt
     7741 tgcgtagcct tgcatgatcc aggttgggat tttggtgtaa ccttcccatg ccgtgtcctg
     7801 taccacttcc cagccgtgct gctgcgcgtg ttgcatggtc aggcgaacgg tatcgtcata
     7861 gttcatatcc gtgacgatgc actcggcacc gaggttcaga atggcgtcaa cgcgttcctg
     7921 agcagaacct ttcggcatgt aaatcaccgc attctgtccg agttgctgtg ctgcccacgc
     7981 cacaccgcgc ccgtggttgc cgtcggtggt ggtcgcgaaa gtcatttttt cgccgatggc
     8041 atttttcagg tgctcaaatg acagcgtttc gatatcaaga tgatattttt cacacaataa
     8101 ttgagcgatg gcgtacgcac cgccaagcat tttgaaggcg ttcagaccga atcgttttga
     8161 ttcgtctttg acgagaattt tcttcacacc aaaaaggttt gcgagatcgt ccagcgcaca
     8221 aagcggtgtt gggcgataac cagctatttt ctggtggaac tggcgcgcca gtttggcctg
     8281 gctttgcgaa aagagcggtg atgtttcgcc gttgaaaaat ttgttatcgg cgatatcaat
     8341 cttcaatgag aaaacggaca tatcctatcc ttttggcctc tggaaataga gggccgacca
     8401 gctgatcggc ccgaggggtt atttcacgcg ttcttgcgct tctttcagca gttgctcgag
     8461 cagtgcacca ggtttggcgt atttacggga caggatcatc gcggcgatga tataaggctt
     8521 ccagctggct tctttgtagg tagcgatacg gtatttttcg aatacgcctt cagtcacttc
     8581 accttctttg caggatacgc cgctgatatc agctggcagg cagtgcatgt acagggcttc
     8641 gccatcacgg gtcagttcca tcatctcttc agtacaatgc cagtctttgt gttgcgcgtt
     8701 ctgtgccaga cactgttttt ccagtgcttt taagccttcg tggtcgttcg cacgcagcaa
     8761 ttcagtacgc tcttccatca ctttgtaagg tgcccatgac ttcggataaa cgatgtctgc
     8821 gtctttgaag gcttcttcca tgctggtgac ctgacggaag ctaccaccgg aggctttagc
     8881 attgtttttc gccacttcaa ccacatccgg gatcaggtcg tagccttccg gatgggccag
     8941 ggtgacatcc ataccgaagc gagtcatcag accgatgatg ccttgtggta cagagagcgg
     9001 tttgccatag cttggagagt aggcccaggt catggcgatt tttttacctt tcaggttttc
     9061 cagtgaacca aagtgttcac gtaaccacgc gaggtcagcc attgactgag tcgggtggtc
     9121 aatatcgcat tgcaggttca ctaaagccgg acgctgtggc agtacaccct gcttgtaacc
     9181 gtcatcaagt gcagcgccaa cttcacgcat ataggcgttg cctgcgccca gatacatatc
     9241 gtcgcgaata ccaatagcgt cggcgcagaa ggagatcata ttggcggttt cacgcacggt
     9301 ttcgccgtga gcgatttgtg atttgccttc gtcgagatct tgttgtgcaa ggccgagcag
     9361 gttaagcgcg gaagcataag agaa
//
LOCUS       DAJVOY010000161         9089 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVOY010000161 DAJVOY010000000
VERSION     DAJVOY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.161"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            52..936
                     /locus_tag="OQL90_003326"
     CDS             52..936
                     /locus_tag="OQL90_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1369990.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OQL90_003327"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OQL90_003327"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCS1369991.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OQL90_003328"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OQL90_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1369992.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OQL90_003329"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OQL90_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1369993.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OQL90_003330"
     CDS             5160..6443
                     /locus_tag="OQL90_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCS1369994.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="OQL90_003331"
     CDS             6590..8065
                     /locus_tag="OQL90_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1369995.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPSQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVSDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            8159..8245
                     /locus_tag="OQL90_003332"
                     /pseudo
     CDS             8159..8245
                     /locus_tag="OQL90_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            8246..>9089
                     /locus_tag="OQL90_003333"
     CDS             8246..>9089
                     /locus_tag="OQL90_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1369996.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
BASE COUNT         2394 a         2181 c         2106 g         2408 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tagtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacatta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca ttcttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgt tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgaccagg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcaggagc tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgcgacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgagtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgccct gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggatcgt atacgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaactgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggcattttat
     8281 cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg tcgccagcga
     8341 cagagcaatg agtgcctatc tgcgctatat cgtgcagaaa gcagactcct cctttcttta
     8401 tgataagtat cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca
     8461 gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt ctgccaataa
     8521 tacccatggc ttaaacctga ctgctcataa atacccgggc ttacgcggca cactacaaac
     8581 cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg cttttgatca
     8641 ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga tggccgaaga
     8701 gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg agccggttaa
     8761 tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta ttggtatcga
     8821 tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca tttaccagga
     8881 tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtgtatgttg cagggcagct
     8941 aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct tttacactca
     9001 tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg attcggggcg
     9061 tgaagtggcc aaattcagtg tgccacttc
//
LOCUS       DAJVOY010000162         9005 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVOY010000162 DAJVOY010000000
VERSION     DAJVOY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.162"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..869
                     /gene="maeB"
                     /locus_tag="OQL90_003334"
     CDS             <1..869
                     /gene="maeB"
                     /locus_tag="OQL90_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1369997.1"
                     /translation="LIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYF
                     QIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSVVKNVFGY
                     RDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVAL
                     LSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDRMPDSPLK
                     GSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASVRRIVNMV
                     ALAVVEAQTQPL"
     gene            1161..1496
                     /gene="eutS"
                     /locus_tag="OQL90_003335"
     CDS             1161..1496
                     /gene="eutS"
                     /locus_tag="OQL90_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS1369998.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            1509..1988
                     /gene="eutP"
                     /locus_tag="OQL90_003336"
     CDS             1509..1988
                     /gene="eutP"
                     /locus_tag="OQL90_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1369999.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            1963..2664
                     /gene="eutQ"
                     /locus_tag="OQL90_003337"
     CDS             1963..2664
                     /gene="eutQ"
                     /locus_tag="OQL90_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCS1370000.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGVLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            2661..3470
                     /gene="eutT"
                     /locus_tag="OQL90_003338"
     CDS             2661..3470
                     /gene="eutT"
                     /locus_tag="OQL90_003338"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCS1370001.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQKPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            3460..4476
                     /gene="pta"
                     /locus_tag="OQL90_003339"
     CDS             3460..4476
                     /gene="pta"
                     /locus_tag="OQL90_003339"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1370002.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            4515..4808
                     /gene="eutM"
                     /locus_tag="OQL90_003340"
     CDS             4515..4808
                     /gene="eutM"
                     /locus_tag="OQL90_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCS1370003.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            4915..5202
                     /gene="eutN"
                     /locus_tag="OQL90_003341"
     CDS             4915..5202
                     /gene="eutN"
                     /locus_tag="OQL90_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCS1370004.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            5214..6617
                     /locus_tag="OQL90_003342"
     CDS             5214..6617
                     /locus_tag="OQL90_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCS1370005.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            6628..7464
                     /gene="eutJ"
                     /locus_tag="OQL90_003343"
     CDS             6628..7464
                     /gene="eutJ"
                     /locus_tag="OQL90_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCS1370006.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            7454..>9005
                     /gene="eutB"
                     /locus_tag="OQL90_003344"
     CDS             7454..>9005
                     /gene="eutB"
                     /locus_tag="OQL90_003344"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HCS1370007.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
                     RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSN
                     ADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAV
                     IGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQS
                     ICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQV
                     TMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDC
                     CYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLNL
                     RPSPEFERW"
BASE COUNT         2055 a         2354 c         2674 g         1922 t
ORIGIN      
        1 tccttatcgg tcgtccgaac gtgatcgaaa tgcgcattca gaaactgggc ttgcagatca
       61 aagcgggcgt tgattttgag atcgtcaata acgaatccga tccgcgcttt aaagagtact
      121 ggaccgaata cttccagatc atgaagcgtc gcggtgtcac tcaggaacag gcgcagcggg
      181 cgctgatcag taacccgaca gtgatcggcg cgatcatggt tcagcgtggg gaagccgatg
      241 caatgatctg cggtacggtg ggcgattatc atgaacattt tagcgtggtg aaaaatgtct
      301 ttggttatcg cgatggcgtt cacaccgcag gtgcaatgaa cgcgctgctg ctgccgagtg
      361 gtaacacctt tattgccgat acctacgtca atgacgaacc agacgcagaa gagctggcgg
      421 agatcacctt gatggcggca gaaactgtcc gtcgttttgg tattgagccg cgcgttgctc
      481 tgttgtcgca ctccaacttt ggttcttctg actgcccgtc gtcgagcaaa atgcgtcagg
      541 cgctggaact ggtcagggat cgtgcgccag atctgatgat tgatggtgaa atgcacggcg
      601 atgcagcgct ggtggaagcg attcgcaacg accgtatgcc ggacagccct ttgaaaggtt
      661 ctgccaatat tctggtgatg ccgaacatgg aagctgcccg cattagttac aacttactgc
      721 gcgtttccag ctcggaaggt gtgactgtcg gtccggtgct gatgggcgtg gcgaaaccgg
      781 ttcacgtgtt aacgccgatc gcatcggtgc gtcgtatcgt caacatggtg gcgctggccg
      841 tggttgaagc gcaaacccaa ccgctgtaat tcttttttaa ctctcacgct taccctgaat
      901 attcagggta agcggtttgg ctgcaatata ttagtaaagc ttattactga gtttgcgaat
      961 aataaaaaag cagtctatat aatatctcga tattatttta tttatattca tgcgttgcat
     1021 atgaaagttt atgcaccaca gcgaatatct ctccattctt agtgatctac ctcacctttt
     1081 aaacgcgctt gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag
     1141 tttttcacag gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca
     1201 aacaggtcac actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg
     1261 gcgttccgga tgcgggcgca attggcatta tgacgctaac tcccggcgaa acggcgatga
     1321 tcgccggtga tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgcttca
     1381 gcggtgcatt ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg
     1441 tcagcggttt aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg
     1501 aggcattcat gaaacgaatt gcgtttgtgg gtagtgtcgg ggcaggaaaa acaacgttat
     1561 ttaatgcgtt gcaggggaat tataccctcg ccagaaaaac acaggccgtg gaatttaatg
     1621 ataagggcga tattgatacg ccgggtgaat attttagcca tccccgctgg tatcacgcct
     1681 taattaccac gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg
     1741 aaagtcggtt gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca
     1801 tcagtaaaac ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga
     1861 cgggtttcga agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac
     1921 tggtggatta tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca
     1981 gcgaatgata ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc
     2041 gccagcatta ttaccccgga agcccgtgaa gtggcggatc tactgggttt taccatcacc
     2101 gaatgtgatg aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa
     2161 accgaaagcc agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc
     2221 gaaagcctgg tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctggagcag
     2281 ggcgcaatgc agccgagctt caaatcggtg accggcaaag gcggcatcaa agtgattgat
     2341 ggcagcagcg tcaagtttgg tcgtttcgac ggcgcagagc cgcattgcgt gggcttgact
     2401 gatcttgtga ccggagacga cggcagcagc atggccgccg gttttatgca gtgggagaac
     2461 gcattcttcc cgtggaccct gaactacgac gaaatcgaca tggtgctgga aggggtgctg
     2521 catgttcgcc acgaaggcca aaccatgatc gccaaagcag gcgacgtgat gtttatcccg
     2581 aaaggctcca gcatcgaatt tggtatgaca tccagcgtga aattcctgta tgtcgcctgg
     2641 ccggctaact ggcaatccct atgaaagatt tcatcaccga agcatggcta agagcgaacc
     2701 atacgctcag cgaaggcgca gagatccatc tccctgcgga cagtcgcctg acgccctctg
     2761 cccgggagtt gctggaaagc cgccatctgc gcatcaagtt tattgacgag cagggccgcc
     2821 tgtttgttga cgatgaacag cagaagccgc agcccgttca tgggctgacc agtagcgatg
     2881 aacatccgca ggcgtgctgc gaactgtgcc gccagccggt ggcaaaaaag ccggatacgc
     2941 tgactcacct gtcggcggaa aaaatggtcg ccaaaagcga tccgcgtctg ggttttcgcg
     3001 cggttctcga cagcaccatt gcgctggcag tgtggctgca aattgaactg gcggaaccgt
     3061 ggcagccgtg gctggcggat atccgttcgc gtcttggcaa cattatgcgc gccgatgcgc
     3121 tgggggaacc gctgggctgc caggcgattg tcgggctttc tgacgaagat ctgcatcggc
     3181 tttctcatca gccgctgcgc tatctcgacc acgatcatct ggtgctggaa gccagccacg
     3241 ggcgcgatgc tgcgttgctc aatctgctac gcaccaaagt gcgggaaacc gaaaccgtag
     3301 cggcgcaggt ctttatcacc cgcagctttg aagtattacg cccggacatt ttgcaggcgc
     3361 tgaaccgcct ttccagcacg gtctacgtga tgatgatcct gagcgtaacg aagcagccgc
     3421 taacggtgaa gcagatccaa caacgactgg gggaacgcaa tgattattga acgttgtcgt
     3481 gaactggcgt tgcgagcgcc cgccagagtg gtttttccgg atgcgttaga tcaacgtgtg
     3541 ctgaaagccg cgcaatattt acatcaacaa ggtctggcaa cgcccattct ggtcgccaat
     3601 ccgtttgaac ttcgtcagtt tgcgctcagt cacggcgtgg cgatggacgg gctacaggtg
     3661 attgatccgc atggcaacct cgcaatgcgg gaagaatttg ctcatcgctg gctggcccgc
     3721 gcgggcgaaa aaacgccgcc ggatgcgctg gaaaaactta ccgaccctct gatgttcgcc
     3781 gcagcaatgg tcagcgccgg taaagcggat gtctgtatcg caggcaacct ctcttccacg
     3841 gcaaatgtgc tgcgtgccgg attacgcatt atcggcttgc agccaggctg taaaacgctc
     3901 tctttcattt tcctgatgtt gccacagtac agcggcccgg cgttgggctt tgccgattgc
     3961 agcgtggtgc cacagccgac ggcggcgcag ctggcggata tcgcgcttgc cagtgccgaa
     4021 acctggcgcg ccatcaccgg agaagagccg cgcgtggcga tgctgtcgtt ttccagcaat
     4081 ggtagcgccc gtcacccctg tgttgctaac gtccagcagg cgacagaaat cgtccgtgag
     4141 cgcgcaccaa agctggtggt ggatggcgag ttacagtttg acgccgcctt cgtgccggaa
     4201 gtggcggcgc aaaaagcgcc tgccagcccg ctacagggca aggccaatgt gatggttttt
     4261 ccgtcgctgg aagccggaaa tattggttac aacatcgcac aacgactcgg cggatatcgt
     4321 gccgtcgggc cactgataca aggacttgcc gtgccgatgc acgatctctc tcgtggttgt
     4381 agcgtgcagg aaattatcga gctggcgctg gtggcagctg tgccgcgtca gacagaagtg
     4441 aaccgcgaaa gcagtttaca aacactggtt gaatgaacgg tcccgttctg gaccctgata
     4501 aagaggaaag cacaatggaa gcattaggaa tgatcgaaac ccggggcctg gttgcactga
     4561 ttgaggcttc tgacgcgatg gtgaaagctg cgcgcgtcaa gctggttggt gttaagcaga
     4621 ttggcggcgg cctgtgtact gccatggttc gtggtgatgt tgcagcctgt aaagccgcga
     4681 ccgatgcagg tgccgcagcg gcacagcgta tcggcgagct ggtatctgta cacgttatcc
     4741 cgcgtccgca cggcgacctg gaagaagtct tcccgatcgg cctgaaaggc gatagcagca
     4801 acctgtaatc accctgtgtg acgtcgattc gggcgttacg ctctctcctt tttcatttta
     4861 ccttctgcgg agggtagggg gagcttttac ctgaaatacg ccacggaggc gggtatgaaa
     4921 ctggcagtcg tcactggaca aattgtttgt accgtacgcc atcacggact ggcgcatgac
     4981 aaattgctga tggtggaaat gattgatcca caaggtaatc ctgacgggca atgcgccgtc
     5041 gccatcgaca atattggcgc gggaaccggg gagtgggtgt tactggtgag tggcagttcc
     5101 gcccgccagg cgcataaaag cgaaacgtca ccggtcgatc tgtgcgtgat tggcattgtc
     5161 gatgaggtgg tgtctggcgg tcaggtaatt ttccacaaat aaggcagaac atcatgaatc
     5221 aacaggatat tgaacaggtg gtgaaagcgg tactgctgaa aatgcaaagc agtgacacgc
     5281 cgcccgccgc cgttcatgag atgggcgttt tcgcgtccct ggatgacgcc gttgcggcag
     5341 ccaaagtcgc ccagcaaggg ttaaaaagcg tggcaatgcg ccagttagcc attgctgcca
     5401 ttcgtgaggc aggcgaaaaa cacgccagag atttagcgga acttgccgtc agtgaaaccg
     5461 gcatgggccg cgttgaagat aaatttgcca aaaacgtcgc tcaggcgcgc ggcacgccag
     5521 gcgttgagtg tctctctccg caggtgctga ccggcgacaa cggcctgact ttaattgaaa
     5581 acgcgccctg gggcgtggtg gcttcggtaa ctccgtccac taacccggcg gcaaccgtaa
     5641 ttaataacgc catcagcctg attgccgcgg gcaacagcgt catttttgcc ccgcatccgg
     5701 cggcgaaaaa agtctcccag cgggcgatca cgctgctcaa ccaggcgatt gttgccgcag
     5761 gcgggccgga aaacttactg gttactgtgg caaatccgga tatcgaaacc gcgcagcgtt
     5821 tgttcaagtt tccgggcatc ggccttctgg tggtgaccgg cggcgaagcg gtagtagaag
     5881 cggcgcgtaa acacaccaat aaacgtctga ttgccgcagg cgctggcaac ccgccggtag
     5941 tggtggatga aaccgccgac ctcgcccgtg ccgctcagtc catcgtcaaa ggcgcttctt
     6001 tcgataacaa catcatttgt gccgacgaaa aggtactgat tgttgttgat agcgtagccg
     6061 atgaactgat gcgtctgatg gaaggccagc acgcggtgaa actgaccgca gaacaggcgc
     6121 agcagctgca accggtgttg ctgaaaaata tcgacgagcg cggaaaaggc accgtcagcc
     6181 gtgactgggt tggtcgcgat gcggcgaaaa tcgcggcggc aatcggcctg aacgtgccgc
     6241 aagaaacgcg tttgctgttt gtggaaacca ctgcagaaca tccgtttgcc gtgaccgaac
     6301 tgatgatgcc ggtgctgccc gtcgtgcgcg tcgccaacgt ggcggatgcc attgcgctgg
     6361 cggtgaaact ggaaggcggt tgccaccaca cggcggcaat gcactcgcgc aacatcgaaa
     6421 acatgaacca gatggcgaat gctattgata ccagcatttt cgttaagaac ggaccgtgca
     6481 ttgccgggct ggggctgggc ggggaaggct ggaccaccat gaccatcacc acgccaaccg
     6541 gtgaaggggt aaccagcgcg cgtacgtttg tccgtctgcg tcgctgtgta ttagtcgatg
     6601 cgtttcgcat tgtttaagga gtaacagatg gcgcacgacg aacaatggct caccccacgt
     6661 ctgcaaacgg cggcaacgct gtgtaaccag acgcccgccg cgacggaatc tccgctgtgg
     6721 ctgggggtgg atctgggcac ctgcgatgtg gtgtcgatgg ttgtcgaccg cgacggtcag
     6781 ccggtggcgg tatgcctcga ctgggccgat gtggtccgcg acggcatcgt ctgggatttc
     6841 ttcggcgctg tcaccattgt tcgtcgccat ctcgacacgc tcgagcagca attcggtcgc
     6901 cgttttagcc atgcggcgac ttcatttcct ccaggtacgg acccgcgtat ttccatcaac
     6961 gtgctggagt ctgctggact ggaagtgagc catgtgctgg atgagccaac cgcagttgcg
     7021 gagctgctgc aactggacaa cgccggtgta gtggatatcg gcggcggcac taccggcatt
     7081 gccatcgtga aaaagggcaa ggtgacgtac tcggcggatg aagcaaccgg cggacatcac
     7141 atctctctga ctctcgccgg aaatcgccgt atttcgctgg aagaggcgga gcagtacaag
     7201 cgcggtcacg gtgaagagat ttggcctacg gtgaaaccgg tgtacgaaaa aatggcggac
     7261 attgtcgccc ggcatattga agggcagggc attactgatt tatggctggc gggcggctct
     7321 tgtatgcaac cgggcgtggc ggaactgttt cgcaaacaat tcccggcgtt acaggtgcat
     7381 ttaccgcagc acagtttgtt tatgaccccg ctggcgatcg ccagtagtgg gcgagagaaa
     7441 gcggaggggc tatatgcaaa atgaattgca gaccgcgctc tttcaggcgt tcgataccct
     7501 gaatctgcaa cgggtaaaaa catttagcgt tccaccggtg acgctttgcg gtccgggctc
     7561 ggtgagcagt tgcggacagc aagcgcaaac gcgtgggctg aaacatctgt tcgtgatggc
     7621 agacagcttt ttgcatcagg cagggatgac cgccgggctg acgcgcagcc tggctgttaa
     7681 aggcatcgcc atgacgctct ggccatgtcc ggtgggcgaa ccgtgcatca ccgacgtgtg
     7741 tgcagccgtg gcgcagttgc gtgagtcagg ctgtgatggg gtgatcgcat ttggcggcgg
     7801 ctcggtgctg gatgcggcga aagccgtggc gttgctggtg acgaacccgg atagcacgct
     7861 ggcagagatg tcagaaacca gcgttctgca accgcgcttg ccgctgattg cctatgaaga
     7921 tgactgcgtg acgcggctga ttcaggacga tgttaacgaa acggcctaca accagattaa
     7981 aaactggagc atcagcgaac tgcgtgagta tgtgctgagc gatgaaacta gcgtggacga
     8041 cattgccttt acccgcaaag ggctgacctc ggaagtggtc gcggcggtag cgaagatttg
     8101 ctccaacgcg gacctgatct acggcgcgaa gaaaatgccg gtaatcaaaa aggccaatac
     8161 caccatcggt attccgggca ccttcagcgc ccgtttgcag ccaaatgaca ctcgtgacga
     8221 cgtgcaaagt atcgccgcgc aaatctacga agggctttcc ttcggggtgg gcgatgcggt
     8281 gatcggcgtc aacccggtaa ctgacgacgt ggaaaactta agccgcgtgc tggataccat
     8341 ttatggcgtg atcgacaaat tcaacatccc aactcagggc tgcgtactgg cgcacgtcac
     8401 cacccagatc gaagcgattc gtcgcggcgc gcctggcgga ctgattttcc agagtatctg
     8461 tggcagcgaa aaagggctga aagagtttgg cgtggaactg gcgatgctcg acgaagcgcg
     8521 cgcagtgggt gcagagttca accgtatcgc cggggaaaac tgcctctact tcgaaaccgg
     8581 acaaggctct gcgctatccg ctggcgctaa cttcggcgct gaccaggtga cgatggaagc
     8641 acgtaactat gggctggcgc gtcattacga tccgtttatc gtcaacaccg tggttggctt
     8701 tattgggccg gagtatctct acaacgaccg ccagattatc cgtgctggct tagaagatca
     8761 ctttatgggc aagctgagcg gcatctctat gggctgtgac tgctgctaca ccaaccacgc
     8821 tgacgctgac cagaacctca acgaaaacct gatgatcctg ctcgccaccg caggctgcaa
     8881 ctacatcatg gggatgccgc tgggtgatga catcatgctc aactaccaga ccaccgcatt
     8941 ccacgatacc gccactgtgc gtcagttact caacctgcgc ccgtcaccag agtttgaacg
     9001 ctggc
//
LOCUS       DAJVOY010000163         8899 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVOY010000163 DAJVOY010000000
VERSION     DAJVOY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.163"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            48..881
                     /gene="ppsR"
                     /locus_tag="OQL90_003345"
     CDS             48..881
                     /gene="ppsR"
                     /locus_tag="OQL90_003345"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1370008.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1037..2083
                     /gene="aroH"
                     /locus_tag="OQL90_003346"
     CDS             1037..2083
                     /gene="aroH"
                     /locus_tag="OQL90_003346"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1370009.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARTSHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2215..2406
                     /gene="hemP"
                     /locus_tag="OQL90_003347"
     CDS             2215..2406
                     /gene="hemP"
                     /locus_tag="OQL90_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1370010.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2410..3846)
                     /locus_tag="OQL90_003348"
     CDS             complement(2410..3846)
                     /locus_tag="OQL90_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1370011.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(3909..4622)
                     /gene="ydiV"
                     /locus_tag="OQL90_003349"
                     /pseudo
     CDS             complement(3909..4622)
                     /gene="ydiV"
                     /locus_tag="OQL90_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            complement(4868..5332)
                     /locus_tag="OQL90_003350"
     CDS             complement(4868..5332)
                     /locus_tag="OQL90_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1370012.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5410..6159)
                     /gene="btuD"
                     /locus_tag="OQL90_003351"
     CDS             complement(5410..6159)
                     /gene="btuD"
                     /locus_tag="OQL90_003351"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1370013.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6159..6710)
                     /gene="btuE"
                     /locus_tag="OQL90_003352"
     CDS             complement(6159..6710)
                     /gene="btuE"
                     /locus_tag="OQL90_003352"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1370014.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6773..7753)
                     /gene="btuC"
                     /locus_tag="OQL90_003353"
     CDS             complement(6773..7753)
                     /gene="btuC"
                     /locus_tag="OQL90_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1370015.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(7962..8291)
                     /locus_tag="OQL90_003354"
     CDS             complement(7962..8291)
                     /locus_tag="OQL90_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370016.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
     gene            complement(8399..8761)
                     /locus_tag="OQL90_003355"
     CDS             complement(8399..8761)
                     /locus_tag="OQL90_003355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370017.1"
                     /translation="MKFHLDELKRMATLDNFARFLNESSTNEKCLSCGDTDMYMYLTN
                     IVEVGPEPKTAEECDLGTFVMLDYIGPFTGYPGYEGHDRENIHNYEFRLTCNRCGFVH
                     RYSARAFMNWVGKQGDEK"
     gene            complement(8764..>8899)
                     /locus_tag="OQL90_003356"
     CDS             complement(8764..>8899)
                     /locus_tag="OQL90_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1370018.1"
                     /translation="MNGGNILTLQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGA
                     "
BASE COUNT         2340 a         2365 c         2031 g         2163 t
ORIGIN      
        1 taatttatgc tttcatagaa ttatgtctgc atcacgggaa gaacaaaatg gataatgctg
       61 ttgatcgcca cgttttttat atttctgatg gtacggcaat aactgcggag gtattaggac
      121 acgcagtaat gtcacaattt cccgtcacta tcagcagcat tacgctgccg tttgtcgaaa
      181 atgagagccg tgcacgggca gtgaaggatc agattgacgc aatttatcac cagacaggtg
      241 tgcgcccgct ggtcttctac tccatcgtgt tgccggagat tcgcgccatc atcttgcaaa
      301 gtgaaggctt ttgccaggat atcgttcagg cgctggttgc cccgctacaa caagagatga
      361 aactggatcc aacgccgatt gctcatcgta cccatggcct taaccctaat aatctcaata
      421 aatatgatgc gcgcattgcg gcgattgatt acacccttgc ccacgatgac ggcatttcgt
      481 tgcgcaatct ggatcaggct caggtgatcc tgctcggtgt ttctcgctgt ggtaaaaccc
      541 ccaccagtct gtatctggca atgcaatttg gtatccgcac ggcaaactac ccctttattg
      601 ccgacgatat ggataatctg gtgctacccg cgtcgctcaa accgcttcag cataaattgt
      661 tcggcctgac tatcgacccg gaacgtctgg cggcgattcg cgaggaacgt cgggagaaca
      721 gtcgctatgc ctcgcttcgt cagtgcagaa tggaagtcgc ggaagtggaa gccttgtacc
      781 gtaaaaatca gatcccgtgg attaacagta ccaattattc ggtagaagag attgccacca
      841 agatcctcga tatcatgggc cttagtcgcc gaatgtacta gagaactagt gcgctggttt
      901 gttttttgtt atcatgctaa ccacccggcg aggtgtgaca cacctcgcac ttgaaatcag
      961 ctgcgattgg tttatcgtga tgcgcatcac ttcccggtag tcctgccgta gaagcaacaa
     1021 atttctgaga cttgtaatga acagaactga cgaactccgt actgcgcgta ttgagagcct
     1081 ggtaacgccc gccgaactcg cgctacggta tcccgtaacg cctggcgtcg ccacccatgt
     1141 caccgactcc cgccgcagaa ttgaaaaaat actgaatggt gaagataagc gactgttggt
     1201 cattattggc ccctgctcga tccacgatct caccgctgca atggagtacg ccacccgtct
     1261 gcagtcgctg cgcaaccagt accagtcacg gctggaaatc gtaatgcgca cctattttga
     1321 aaaaccacga actgttgtcg gctggaaagg gctaatctcc gatccggatt taaacggcag
     1381 ctatcgggta aatcacggtc tggagctggc gcgcaaatta cttttacagg taaatgagct
     1441 gggtgtccct accgcgactg agtttctcga tatggtgacc ggtcagttta ttgctgattt
     1501 aatcagttgg ggcgcgattg gcgctcgtac taccgaaagt cagatccacc gcgaaatggc
     1561 ttcagcactc tcctgtccgg taggttttaa aaatggtacc gatggcaaca cgcggattgc
     1621 cgtggatgct atccgcgcag cccgcaccag ccatatgttc ctctcgccag acaaaaatgg
     1681 tcagatgacc atctatcaga ccagcggcaa cccgtatggc cacattatta tgcgtggcgg
     1741 caaaaaaccg aattatcatg ccgatgatat cgccgcagcc tgcgatacgc tgcacgagtt
     1801 tgatttacct gaacatctgg tggtggattt cagccacggt aactgccaga agcagcaccg
     1861 tcgccagtta gaagtttgtg aggatatttg tcagcaaatc cgcaatggct ctacggcgat
     1921 tgctggaatt atggcggaaa gtttcctgcg cgaaggaacg caaaaaatcg tcggcggtca
     1981 gccgctcact tacggtcaat ccattaccga cccgtgtctg ggctgggaag ataccgaacg
     2041 cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc tgaatgcgtg cccatgcctg
     2101 atgggatggg catttctgcg caacttgttg tcttctcaac acattactgc ttgctctggc
     2161 ctgccataat attgataata agaatcattg ttatatcaat tattattaat ttttatgcgt
     2221 tatacggata gcagaaaact cacgcctgaa acggatgcca atcacaagac cgcttccccg
     2281 cagcccatcc ggcgaattac cagccagaca ctgttaggtc cggatggcaa actgattatc
     2341 gatcatgacg ggcaagaata tctgctccgt aaaactcagg ctggcaagct gctgttaact
     2401 aagtagcctt taactcgagc agctgacttc cagccgttta ccccagtcag gtggacgact
     2461 gacatagtca tcatctctgt cgctgaaagg atttcgcaac gcctcatgca ggcggtgcaa
     2521 ttccatcata tcaccctttt ctgccgcttc aatcgcccgt tgcgccaacc agttgcgcaa
     2581 taccagagct gggttaacgc tttgtatcag ttgctgacgc tcactatcgc taacctcgtc
     2641 ttgttgcaaa cgcccccgat aacgggcaaa ccagtcatca aatgccgcac gatcaataaa
     2701 ctcatcacgt agcggtgacg ccgcgctgtg ctgctcggtc agactcagca tgcggaatgt
     2761 gcgggtataa tcgctgcgct ctcgcgccat cagactgaat aattcattca gtagcgcgtt
     2821 atcctctttt tgctccgtca tgaagcccag tttctgccgc atccgttgtc cataatgcgt
     2881 caacaaaacc tgctgatagc tgtccagggc ctcattcagg gcatctacgg caacaaatgg
     2941 tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg acagcaggtt gattatcaaa
     3001 gctataacgc ccttgatgat ccgagtgatt acaaataaaa ccgggttcgt aatcatcaag
     3061 aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc gacatgttgt cggtattcat
     3121 caccccatgt gcaaagccga ccgtctgcca ttgggcaatt aacgacgcgg tacgtgcgac
     3181 aacatcgcta aaccagagac ggtatttgtc ctcatcatct tcaagatgtg accagtaatg
     3241 acgaatagca aagtcagcta actgacgaac cttttccgac tcgcggcggt agtaaaaatg
     3301 ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc atcagcatcg cgcctggctc
     3361 cgccgtttcc cgatacactg gcgaatcgct ggtgacgata cttaacgcgc gggtcgtcgg
     3421 aatgcccaga taatgcatcg cctcactggc gagactttct cgtatcgtcg aacgtaaaac
     3481 cgcccgtcca tcacccattc gcgaataagg cgtcaggcca gcacctttca gatgccagtc
     3541 cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa atgccgcgcc catcacccag
     3601 ttggcccgcc cagacgccga actgatgacc actgtaaacc tgcgccagtg gtgacatacc
     3661 aggcagtagg gcttcgccgc cccagacacc tgcgccattt ttaaacagcg acgatggaat
     3721 actcagcgtg ttagccagtt cgatattatg ccaaatcagc cgggcattat ttaaaggcgt
     3781 aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc cagcgggtaa caaaagacag
     3841 ggtcatagat cctcctgttt gatagtgtag acggttactc tcgtcttaaa caccagcaaa
     3901 cagaagggtt atcgctgaac caacgtcgtt atctgattta tgggaacggc aggccacagg
     3961 catccctgga gagcatggaa atcgaagggg gtaatctgtg ccaatatttc tgccgtatca
     4021 atgcctcccg caatgatgca attgcaacaa ggggaaattt gcgcctagat cgcacgaata
     4081 aacggttcga aggagcggtg cgtgatttgt tgctgaataa aacttttatc cagcatgacg
     4141 cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat tgcctgctcc caaattacct
     4201 aacacaaggg gatacacttg cgataaggat aaaagaccgc gattatcttt tccttcattg
     4261 agatgcgggt aattctcatt aatcaacagt tcaataaacg gatattttaa tagttcgcct
     4321 gcgtaattat ccctctctaa taataatgta gccacttgtg gcgttaaatt aagccaggca
     4381 aagagtttat gttgaataaa aaaatgttgg catgatttta gtaattccaa ctgctctgaa
     4441 aataattgcc aatgttgctc ctcggtaagt tgcgctataa cccgactcgt cgggatacgc
     4501 accgtgccat cttcactgga gaaatgggta attaactcaa cgccgacaag gacttgctgg
     4561 ttatctctta tcggcaggaa atagcaatca gaatgataaa gattctccaa aaaaatcttc
     4621 atggtaaccg tccctctcga aggatgtttt tcagtatccg gctaaaaggg ataaggccat
     4681 aagacataac aattacagaa ggagtaactt tcatttgttc catgttaacc actttttcag
     4741 gggtcttttt ttagattatc ctgattataa acgaataatc catttacgga atttttgtct
     4801 gcaaaatact actgtattca ggagtaaatg gacggcgatg tatcatcgcc gtccattttt
     4861 cgacatacta gattcgtctc gcctgccaga aatttttctg ccaatagaca ttatcaagtg
     4921 aggaacgcat cactcccttg ctggtagagg cgtggataaa ttggttgttg gtatcgtaaa
     4981 tacctacatg caaaccattt tgtccggacc ccgttttgaa aaagaccagg tcacctggca
     5041 gcaacttgtc tttatcaatt tgcgtgccaa tagaggcttg ttgtttggtt tcacggggca
     5101 gctgcaaatc gaaacgatcg cgcatcgtca caaccacaaa tcccgaacag tccacaccgc
     5161 gccgcgtcat gccaccataa cgatacggcg tgccatgcca gctttgtagc tggtcgttca
     5221 aaccggcaat aacggtaatc gaatcagaaa gtctggcatt tggcggcggt gctttatggt
     5281 ggctacaccc ggccagaaac agtgctgtga tcaaaataag gcagaaacgc attccgtacg
     5341 gttcctctgt tttttattct tgcattaatt tagcgtcgta attacccgat tttcaagata
     5401 ctaatgaaat cagatggtca aaatcagcat tctgtgacct tcgatatcca gacggcgaaa
     5461 attcatccca taggcctgcg ccagatttgg cggcgtgagc acctcttctc tgcgtccact
     5521 ggccagcatt tttccacctt ttagcaacca cgcccgatgc gcatgacgca acgtgtggtt
     5581 gagatcgtga ctgctcatca caatcgccag tccttgctga cacagcgcgc tcagaatttt
     5641 gtctaacgca ctttgttgcg caacatcaag actgttcatc ggctcatcaa gaagcagcaa
     5701 ttggcctgcg ggattggctt gtggtgtgat ttgcaacacc accgcagcaa gacgtacgcg
     5761 ttgccattca ccgccggaaa gttgattggt gctacgaccg agtttgtcat caagagccag
     5821 cgcccctgcg acatcattca gtagttcggt acgcgtttta tcgtgctgat gcagtgtcag
     5881 gtagtgccag accggcattg caaacggcgg cgtctgctgt tgtgaaagat aggcgcgatg
     5941 cagcgcgagt tttgttgcgg accatgcttc caggggttgc cccgcgaact gaatgcttcc
     6001 cttaccgctg gtcattccgg ccattcgtgc aagcaaggta ctcttacccg cgccattcgg
     6061 ccccaccagg tgcaggatct ccccagcccg aacctcgcca gaaagtggcc ccaggcgggt
     6121 agattccgca acatcctgta actgcatcac aatagacatt attttgccaa cgccagttta
     6181 atgctttcca tcacaatggg gtcttccggc gtcatatccg gggaaaaacg ctggatgacc
     6241 aatccgtccc tgccaactaa aaatttctca aaattccata aaatatcatc agggtacagc
     6301 ggtgcacgac ctttgctgac catacgggca tagaatccgc tctcttccgg cgcgactgcg
     6361 gtcggcgctg cggcaatcaa tttttgatac agcggatggc gtccttcgcc gttaacttca
     6421 atcttactga acatcgggaa cgtcaccccc catgtggtgg tacagtaagt tttaatctct
     6481 tcatcgctgc ccggttcttg ttccagaaac tggttgcacg ggaatcccag caccacaaaa
     6541 cctcgatcgg cccaggcttt ctgaatattc tccaactgct catattgcgg cgttaagcca
     6601 cactttgagg cgacattgac aatcaacagc acattaccgg cgtacttctc cagcgtggtc
     6661 acttcaccgt cgatatcttt cactacggtc gtcagaatgg aatcttgcat cgtttctcct
     6721 gggtgtggtc agtaaaaatc ttagctttta atcatagacc gtctttttgc ggctaacgtc
     6781 ttgcttttaa caataaccag ataaacaccg gcgcacccaa cgttgcggtg accacgccaa
     6841 taagcagctc tgcggcagct aatgccaggc gcgctacaat atcggccagc agcaatgcgc
     6901 tcgcccctgc cagcgcgcag ccgggaagta atacgcgatg atcggttaaa ccacacaacc
     6961 gaagaatatg tgggatcacc agaccaataa agccgatagc acccgccagc gccacactga
     7021 cgccaaccat ccagccggtc gcggccacca gcacattgcg ccagaaccac aggggtaaac
     7081 ccagttgccg cgccgagatc tcgccaagtg ctaacatatt catcggcctg gactgacaac
     7141 agatccacaa caacacgggg atcaatgcca gcatcagcca gctttgccgc cagtctacgc
     7201 cgccaaaacc gcccatcatc cagtacatca gctgacgtaa atcaacagag gtggaaaagt
     7261 agatagccca cgtcattagt gcgctacaga taatccctaa tgcaacgcca gccagcaata
     7321 accgactggt cgaaagatga cgacgggcga aacgtaagag tattaaagta atgataagcg
     7381 cgccagcaat cgcacacagc cctagcgccc agttggggag ttgcccttgc ccaagcaata
     7441 ccgcggcgat aagccccacg cctgcgccgt tagagacgcc aagtagtcca ggttctgcca
     7501 gaggattttc aaacaacgcc tgcattacag cgccggatat agccagcgcc gcaccaacca
     7561 acaatacagc cagcgtacgt ggcaggcgaa tttgccagac gaacagttcg ccacgaggag
     7621 taaaccagtc acctagcgag atccattgtt caccggcgca aaggcttaaa agaagcgcca
     7681 gcagcatcaa aactgacagg cataataacc agcgaatatt ttgtcgctgt tgttggcggg
     7741 caagtgtcag catggtatcc gttctgctga agtgtcatgg cgttgatttt acggtgactc
     7801 ttcgacagtg aaaagaaaaa aggccgcaga gcggccataa acacaagtaa aaatcaacaa
     7861 gttagataat tatcaaagac ttacagacac acaaaaacac agccaaccac aacaaataac
     7921 agggatgtgg tcactttgtg gatcatatcg ccgccataaa tttacttaat aacagtaacc
     7981 acagccacaa gcacggccat cgcagacagg acaatacctg tgagtaacca agtctgattt
     8041 gcaaatgatt tctgaagttc agtacgatgc tcagccatct caactttcag agactggcgt
     8101 aaatcagcca tctcagactt cagggattgg cgcgattctg ccatctcaag cctcaaccct
     8161 tcgcgaatct ccagtacatc agatttggta gcaaaggatt cgcttcttgt ggtgagagta
     8221 atcaggttgt ttttgatttc agtcacatca ctttccaggc aactgacccg ccgttcaagg
     8281 tcgtcattca tgccatcacc tccatcatta ccacttcctg ttacttggtt gttggataca
     8341 ttataggcat tctctttctt ttttctgaac cgcgaaatat gtgcaacatt ctctgccatc
     8401 acttctcatc accctgcttg cctacccagt tcataaaggc ccgggccgag taacgatgaa
     8461 caaacccaca tctgttacag gtaagtcgga attcatagtt atggatattt tcccgatcat
     8521 gtccctcata tcccggatac ccagtaaatg gaccgatgta atcaagcata acaaaagtgc
     8581 ctaaatcaca ctcctcagca gttttcggct caggccccac ctcaacaata ttcgtcaaat
     8641 acatgtacat atccgtatcg ccacacgaca aacatttttc attcgtagac gactcattga
     8701 gaaaacgcgc aaaattatcg agcgttgcca ttcttttaag ttcatctaaa tgaaacttca
     8761 ctcttaagca cctcctagtg gattaaacct caccgcatcc tgcaggtaat ccggcgcaag
     8821 atgggcataa atcatcgttg tctgaatctt tgcgtgcccc agaattttct ggagcgtcag
     8881 aatattgccg ccgttcatc
//
LOCUS       DAJVOY010000164         8735 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVOY010000164 DAJVOY010000000
VERSION     DAJVOY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.164"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            245..1156
                     /gene="ttdA"
                     /locus_tag="OQL90_003357"
     CDS             245..1156
                     /gene="ttdA"
                     /locus_tag="OQL90_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCS1370019.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1153..1758
                     /gene="ttdB"
                     /locus_tag="OQL90_003358"
     CDS             1153..1758
                     /gene="ttdB"
                     /locus_tag="OQL90_003358"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1370020.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1807..3270
                     /gene="ttdT"
                     /locus_tag="OQL90_003359"
     CDS             1807..3270
                     /gene="ttdT"
                     /locus_tag="OQL90_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1370021.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3313..4326)
                     /gene="tsaD"
                     /locus_tag="OQL90_003360"
     CDS             complement(3313..4326)
                     /gene="tsaD"
                     /locus_tag="OQL90_003360"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1370022.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4563..4778
                     /gene="rpsU"
                     /locus_tag="OQL90_003361"
     CDS             4563..4778
                     /gene="rpsU"
                     /locus_tag="OQL90_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1370023.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4889..6634
                     /gene="dnaG"
                     /locus_tag="OQL90_003362"
     CDS             4889..6634
                     /gene="dnaG"
                     /locus_tag="OQL90_003362"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1370024.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6829..8670
                     /gene="rpoD"
                     /locus_tag="OQL90_003363"
     CDS             6829..8670
                     /gene="rpoD"
                     /locus_tag="OQL90_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1370025.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2110 a         2330 c         2334 g         1961 t
ORIGIN      
        1 acctgcaagt ctttggctaa aggccagcta ttcagcatcg ttaattatcc gcagttgtga
       61 taagcgcagt gtatttcgca aaacattgcc attccctgct ctggcgcaat attcattctt
      121 attttcgcgt tgattcacga actgttaatt acatattcac ggtggcaaaa aatataaaac
      181 cacattttta gtggtagttt atggcggtga atttttccag acaaatacaa aaactggagt
      241 tgccatgatg agcgaaagta ataagcaaca ggcagtgaat aagttgacag agattgtcgc
      301 taactttacc gccatgattt ctacccgaat gcctgatgac gtggtggata aactaaaaca
      361 gctaaaggat gccgaaacgt cgtcgatggg gaaaattatc taccatacga tgttcgacaa
      421 catgcaaaaa gcgattgacc tgaatcgtcc tgcctgtcag gacaccgggg agattatgtt
      481 cttcgttaaa gtcggttccc gcttcccact gcttggcgag ctgcaaagca tactcaaaca
      541 agccgtggaa gaggcaaccg tcaaagcgcc actacgtcac aatgcggtag aaatttttga
      601 cgaagtaaac accggcaaaa ataccggtag cggcgtaccg tgggtcacct gggacatcat
      661 ccccgacaat gacgatgcgg aaatcgaagt ttacatggca ggtggcggct gcacgctacc
      721 tggccgctcg aaagtgttaa tgccgtcaga aggctacgaa ggcgtggtga aattcgtctt
      781 cgaaaatatc tccaccctcg ccgtaaacgc ctgtccaccg gtactggtgg gcgtgggcat
      841 cgccacctcg gtggaaaccg ccgccgtact ctcgcgtaaa gccattttgc gcccgattgg
      901 cagccgccat cccaatccaa aagcggcaga actggagcta cgcctggaag aaggactcaa
      961 ccgtctgggg attggtccac aagggctgac tggcaacagt tcagtgatgg gcgtacatat
     1021 cgaatctgcc gcccgccatc cgtcaaccat cggcgttgct gtctctaccg gctgctgggc
     1081 gcatcgtcgc ggcacactgc tggttcatgc cgatctcacc tttgaaaatc tgtctcacac
     1141 ccggagcgcg ttatgaaaaa gatcctgaca accccgatca aagctgaaga tctgcaagat
     1201 attcgcgtcg gcgatgtgat ctacctgacc ggtacgctgg tgacctgccg tgacgtttgt
     1261 caccgccgct tgatcgaact gaaacgtccg atcccttacg atctcaacgg caaagcgatt
     1321 ttccacgctg gccccatcgt gcgcaaaaac ggcgataaat gggagatggt ttccgttggc
     1381 ccgacaacca gtatgcgtat ggaaagtttt gaacgtgaat ttatcgagca gaccggcgtg
     1441 aaactggtgg ttggcaaagg cggaatgggg ccgctgaccg aagaaggctg ccagaaattc
     1501 aaggcgctac atgtgatttt cccggcaggc tgcgcggtgc tggcggcaac ccaggtggaa
     1561 gagattgaag aagtgcactg gacagagctc ggaatgccgg agtcgctgtg ggtctgccgg
     1621 ggcaaagagt tcggcccgct gattgtctct attgataccc acggcaacaa tctgatagcc
     1681 gaaaacaaaa agctgttcgc cgaacgccgc gatcccatcg tggaagagat ctgcgagcac
     1741 gtccattaca tcaaataacc ctcccggaga ggctcgcccc ctctcctttt tcgcaggcat
     1801 aacacgatga aaccttccac tgaatggtgg cgataccttg cgccgctggc ggtcatcgcc
     1861 attattgctc taattccggt tcccgcaggg ctggagagtc atacctggct ctactttgcc
     1921 gtttttactg gcgtgatcgt tggactgatc ctcgaacccg tgccgggtgc cgtggtggcg
     1981 atggtgggta tctccatcat cgccatactc tctccctggc tgctgttcag ccccgaacag
     2041 ctcgcccagc caggctttaa gttcaccgcc aaatccctct cctgggcagt ttttggtttt
     2101 tctaattcgg ttatctggct cattttcgcc gcctttatgt ttggcacagg ctatgaaaaa
     2161 accgggcttg gacgccgcat tgcgctgatt ctggtgaaaa agatgggaca tcgcacgctg
     2221 tttctcggct atgcggtgat gttctccgag ctgatcctcg cacctgtaac accgtccaac
     2281 tcggcgcgtg gtgcggggat tatctatccc atcatccgta acctgccacc gctctatcaa
     2341 tcacaaccaa acgacagcag ttcgcgcagc attggctcgt acatcatgtg gatggggatt
     2401 gttgccgact gcgtgaccag cgccattttc ttgacggcga tggcaccaaa cttgctgtta
     2461 attggactga tgaaaagcgc atctcacgcc acgctgagtt ggggcgactg gttcctcggg
     2521 atgttgccgc tcagtatttt gctggtgctg ctggttccct ggctggctta tgtgctgtac
     2581 ccgccggtac tgaagtctgg cgatcaggtg ccgcgctggg cagagacgga actgcaggca
     2641 atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc tgatggtagg cgcgctggtg
     2701 ctgtggattt tcggcggtga ttatatcgat gccgcgatgg tcggttacag cgtggtggca
     2761 ctgatgctgc ttctgcgcat tatcagttgg gacgacattg tcagtaataa agcggcgtgg
     2821 aacgttttct tctggctggc ctcgcttatc accctcgcta ccggactcaa caacactggt
     2881 tttattagct ggtttggcaa actgttagca ggcagcttaa gcggttattc gccaacgatg
     2941 gtgatggtgg cgttgattgt ggtgttttat ctgctgcgct actttttcgc cagcgccacg
     3001 gcgtatacct ccgccctcgc accgatgatg attgccgctg cgctggcgat gccggaaatc
     3061 ccgctgccgg tgttctgcct aatggttggc gcggcaattg gtctggggag cattcttaca
     3121 ccatacgcca ccggacccag tccgatttac tacggtagtg gttatctgcc aacggcggat
     3181 tactggcgac tgggggcgat ttttgggctg atattcctcg tgttgctggt gattaccggc
     3241 ttactgtgga tgcccgtggt gttgctttaa gtgaggacgg tgccggaaat tgctctccgg
     3301 cacccaatag ttttacgcag ccggtaactc cgccagcggc cagcgcggac gcacgctaac
     3361 gccgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt ccggcatagg cgatcatcgc
     3421 gccgttatca gtacaaaact ccggacgcgc gtagaacact tcgccgcggc gttttttcat
     3481 catttccgcc agcttcgccc gtaacgtgcg gttagcactc acgccgcccg ccatgaccag
     3541 tcgcttaaag cccgtctgat ccaacgctcg cttacactta atcatcaacg tatcgaccac
     3601 cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg tcgtcggtgc cgttgtcacg
     3661 aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg aaatccagcc ccggacggtc
     3721 ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc tgagccgcca ttttcgacag
     3781 taacggtccg ccaggataat ccagccccag cagcttcgcg gttttatcaa acgcttcacc
     3841 ggcggcatca tcgatagact cgccgagcag ctcgtactga ccaatgccag tcacgctgat
     3901 taactgcgta tgaccgccgg acaccagcag cgcgacaaac ggaaattccg gcgggttatc
     3961 ttccagcatc ggcgctaaca gatgcccttc catatggtgt accgggattg ccggaacgtt
     4021 ccaggcaaac gccagagaac gccccacggt tgcgccaacc agcagtgcgc cgactaatcc
     4081 agggcctgcg gtataggcca cagcatcaat gtcttttgcc gttaagccag actcctttag
     4141 cgccgcctgg atcaacggta cggttttgcg cacgtgatcg cgggaggcca gttcaggcac
     4201 gacgccgccg tagtcagcgt gcaatttcac ctgactatac aattggttgg ctaacaaacc
     4261 tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag gaagtttcaa tacccagtac
     4321 acgcatgact tgttttacct cgctttatta ccgcgcagtg taggaccaat gcgggttgat
     4381 gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac tcctcaccct tataaaagtc
     4441 cctttcaaaa aggccgcggt gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc
     4501 attttgaaat aagctggcgt tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc
     4561 acatgccggt aattaaagta cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca
     4621 agcgttcctg cgaaaaagca ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa
     4681 aaccgactac cgaacgtaag cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac
     4741 tggctcgcga aaacgcacgc cgcactcgtc tgtactaatt ccccgagagc gttgctctcc
     4801 gatcagaccg agttgtagtt gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc
     4861 gtttatgaat tgctaaaaat cggggcctat ggctggacga atcccacgcg tattcattaa
     4921 tgatctactg gcacgcactg acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa
     4981 gcagggcaag aatttccacg cgtgttgtcc attccacaac gagaaaaccc cgtcattcac
     5041 cgttaacggt gagaaacagt tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat
     5101 cgacttcctg atgaactacg acaagcttga gttcgtcgaa acggtcgaag agctggcagc
     5161 aatgcacaat cttgaagtgc catttgaagc aggcagcggc cccagccaga tcgagcgcca
     5221 tcagaggcaa acgctttatc agttgatgga cggtctgaat acgttttacc aacaatcttt
     5281 acaacaacct gttgccacgt ctgcgcgcca gtatctggaa aaacgcggat taagccacga
     5341 ggttatcgct cgctttgcga ttggttttgc gccccccggc tgggacaacg tcctgaagcg
     5401 gtttggcggc aatccagaaa atcgccagtc attgattgat gcggggatgt tggtcactaa
     5461 cgatcaggga cgcagttacg atcgtttccg cgagcgggtg atgttcccca ttcgcgataa
     5521 acgcggtcgg gtgattggtt ttggcgggcg cgtgctgggc aacgataccc ccaaatacct
     5581 gaactcgccg gaaacagaca ttttccataa aggccgccag ctttacggtc tttatgaagc
     5641 gcagcaggat aacgctgaac ccaatcgtct gcttgtggtc gaaggctata tggacgtggt
     5701 ggcgctggcg caatacggca ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc
     5761 cgatcacata caactgttgt tccgcgcgac caacaatgtc atttgctgtt atgacggcga
     5821 ccgtgcaggc cgtgatgccg catggcgagc gctggaaacg gcgctgcctt acatgacaga
     5881 cggccgtcag ctacgcttta tgtttttgcc ggatggcgaa gaccctgaca cgctggtacg
     5941 aaaagaaggt aaagaagcgt ttgaagcgcg gatggagcag gcgatgccac tctccgcatt
     6001 tctgtttaac agcctgatgc cgcaagttga tctgagtacc cctgacgggc gcgcacgttt
     6061 gagtacgctg gcactgccat tgatatcgca agtgccgggc gaaacgctgc gaatatatct
     6121 tcgtcaggaa ttaggcaaca aattaggcat acttgatgac agccagcttg aacgattaat
     6181 gccaaaagcg gcagagagcg gcgtttctcg ccctgttccg cagctaaaac gcacgaccat
     6241 gcgtatactt atagggttgc tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct
     6301 tgagaatctg gatgaaaata agctccctgg acttggctta ttcagagaac tggtcaacac
     6361 ttgtctctcc cagccaggtc tgaccaccgg gcaactttta gagcactatc gtggtacaaa
     6421 taatgctgcc acccttgaaa aactgtcgat gtgggacgat atagcagata agaatattgc
     6481 tgagcaaacc ttcaccgact cactcaacca tatgtttgat tcgctgcttg aactgcgcca
     6541 ggaagagtta atcgctcgtg agcgcacgca tggtttaagc aacgaagaac gcctggagct
     6601 ctggacatta aaccaggagc tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg
     6661 atcgggaagc ccccgacagc cgcactgaga ggcagcggca aatatataag tacgccctcg
     6721 taattatcgt tggcggtaaa caaccgttgg atttcagcgt taacggctga aggacatcgg
     6781 gtcaatcgcc caacaccaac ctcatgaaat aagtgtggat accgtcttat ggagcaaaac
     6841 ccgcagtcac agctgaaact tcttgtcacc cgtggtaagg agcaaggcta tctgacctat
     6901 gccgaggtca atgaccatct gccggaagat atcgtcgatt cagatcagat cgaagacatc
     6961 atccaaatga tcaacgacat gggcattcag gtgatggaag aagcaccgga tgccgatgat
     7021 ctgatgctgg ctgaaaacac cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt
     7081 tccagcgtgg aatctgaaat cgggcgcacg actgacccgg tacgcatgta catgcgtgaa
     7141 atgggcaccg ttgaactgtt gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa
     7201 gacgggatca accaggttca atgctccgtt gctgaatatc cggaagcgat cacctatctg
     7261 ctggaacagt acgatcgtgt tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc
     7321 tttgttgacc cgaacgcaga agaagatctg gcacctaccg ccactcacgt cggttctgag
     7381 ctttcccagg aagatctgga cgatgacgaa gatgaagacg aagaagatgg cgatgacgac
     7441 agcgccgatg atgacaacag catcgacccg gaactggctc gcgaaaaatt tgcggaacta
     7501 cgcgctcagt acgttgtaac gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc
     7561 gctcaggaag agatcctgaa actgtctgaa gtattcaaac agttccgcct ggtgccgaag
     7621 cagtttgact acctggtcaa cagcatgcgc gtcatgatgg accgcgttcg tacgcaagaa
     7681 cgtctgatca tgaagctctg cgttgagcag tgcaaaatgc cgaagaaaaa cttcattacc
     7741 ctgtttaccg gcaacgaaac cagcgatacc tggttcaacg cggcaattgc gatgaacaag
     7801 ccgtggtcgg aaaaactgca cgatgtctct gaagaagtgc atcgcgccct gcagaaactg
     7861 cagcagattg aagaagaaac cggcctgacc atcgagcagg ttaaagatat caaccgtcgt
     7921 atgtccatcg gtgaagcgaa agcccgccgt gcgaagaaag agatggttga agcgaactta
     7981 cgtctggtta tttctatcgc caagaaatac accaaccgtg gcttgcagtt ccttgacctg
     8041 attcaggaag gcaacatcgg tctgatgaaa gcggttgata aattcgaata ccgccgtggt
     8101 tacaagttct ccacctacgc aacctggtgg atccgtcagg cgatcacccg ttctatcgcg
     8161 gatcaggcgc gcaccatccg tattccggtg catatgattg agactatcaa caaactcaac
     8221 cgtatttctc gccagatgct gcaagagatg ggccgtgaac cgacgccgga agaactggct
     8281 gaacgtatgc tgatgccgga agacaagatc cgcaaagtgc tgaagatcgc caaagagcca
     8341 atctccatgg aaacgccgat cggtgatgat gaagattcgc atctggggga tttcatcgag
     8401 gataccaccc tcgagctgcc gctggattct gcgaccaccg aaagcctgcg tgcggcaacg
     8461 cacgacgtgc tggctggcct gaccgcgcgt gaagcgaaag ttctgcgtat gcgtttcggt
     8521 atcgatatga acaccgacca cacgctggaa gaagtgggta aacagttcga cgttacccgc
     8581 gaacgtatcc gtcagatcga agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct
     8641 gaagtgttgc gtagcttcct ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca
     8701 cccggcacta gtccctctgc acaaacgcca ccttt
//
LOCUS       DAJVOY010000165         8650 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVOY010000165 DAJVOY010000000
VERSION     DAJVOY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.165"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..269)
                     /locus_tag="OQL90_003364"
     CDS             complement(<1..269)
                     /locus_tag="OQL90_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1370026.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVW"
     gene            complement(363..925)
                     /locus_tag="OQL90_003365"
                     /pseudo
     CDS             complement(363..925)
                     /locus_tag="OQL90_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1129..1335)
                     /locus_tag="OQL90_003366"
     CDS             complement(1129..1335)
                     /locus_tag="OQL90_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCS1370027.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1442..2005)
                     /locus_tag="OQL90_003367"
     CDS             complement(1442..2005)
                     /locus_tag="OQL90_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1370028.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3380..5215)
                     /locus_tag="OQL90_003368"
     CDS             complement(3380..5215)
                     /locus_tag="OQL90_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370029.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5316..5603
                     /locus_tag="OQL90_003369"
     CDS             5316..5603
                     /locus_tag="OQL90_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1370030.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5575..7104
                     /locus_tag="OQL90_003370"
     CDS             5575..7104
                     /locus_tag="OQL90_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCS1370031.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7274..8491)
                     /locus_tag="OQL90_003371"
     CDS             complement(7274..8491)
                     /locus_tag="OQL90_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1370032.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
BASE COUNT         2682 a         1955 c         1616 g         2397 t
ORIGIN      
        1 caccagacat cgttattaag tagcagcacc ataagataag gagtggcatc gttagccaca
       61 gcttccctac tccagaggta atataaaggg gtgggctcga ctgatttatc tttacgtcgc
      121 ttacactgca aatgttcaga aattagtcta tgcagctcac cagtaaaatc agcaatcaga
      181 gaaggaattg ccttattcat accagggcaa ggtattaatt taaactgtaa taatttaatt
      241 ttaggatgtg tagcagcagc ccgatacaga gttgcaagga cacacttttg ccagagggcg
      301 ttacaggaaa gcttaatgtt tgattctgta tacataataa atcaccttat agttacaata
      361 ggtcaaaaac cgctgtagcc aaagttacgc tggcctgatg ctttagtgtc gagcttcgtc
      421 aaataatcca gacgtaccaa taagcgctgg tactgctctg ggaaattagg atcgttaata
      481 tccaggatgt aacgtccatt agcagggaaa tgaactaatc cctggtgatc ttccagttcc
      541 aaccctaagg cactgtacca ggctccggta attaaccctc tcagggtgtc agagcgctca
      601 tagtttccca gatggtaata ggcgtctttg ttaaaaaaag gcagacgtga taatgacatt
      661 taaaggactg cgtatattct ttcgcccaga taatacaaac cgacgttgcg taggtgcgtt
      721 tgccttgttg ttttttacgg tgttgatctg cattaatttt cgcattcagc gagttacgga
      781 aacgagatat ctctccgggg atcaggttag ggaaacaggt aatgatatcc ccgttgtcaa
      841 tcacaggagg gtaatggaga tccccccgta ttgctgttat tctggaagtg tttttaatat
      901 tcgtattgat agttctcaga gatatatcac actctagata ttttaattaa gtatcagttc
      961 gatatcggca tattgtacac tacctcaggt agatgatgag atcagataca gggatcaact
     1021 gacggtgagc gcagtcccca cattttatcc agggcttact gcatattccc aacagccatt
     1081 cgttagccag gtagacgaat accctgattt gccgctggtt ttgctttcct atcgaagggc
     1141 ccatacatca tcacgcccac gaaacaaccg acgaaataac gcaactttct catccgtgga
     1201 taatacgaaa acgcactgca cagggctctg cggtttacgt cgccattcaa tcccatgagc
     1261 ttcaagtaat gagaccagcc gggtattttc tgcccgcagt actgctagtt catcgctgtc
     1321 agatttataa accatatgcg ttatcacttg tacctgtctc ccggcggcac cacgactgaa
     1381 attcgtgtga aggcaaatcc acgcgataac ttacgatagt tcggacataa gcccccataa
     1441 ttcagttatg tcgtggtcga ccccggttac ttttttcggg aagcctgcta cggcccagaa
     1501 taataccgtg ttctttctga tcctgtttag ccagatagct aagggcgtaa cgtaagtcgt
     1561 ctaccgcaga tttatcacta tagtgaatca cgtgatcgat acgcaccggg tatttgtctt
     1621 tagcccggca cagatgaaaa taaccctccc cttccgtgat ccgcctccag atatctccca
     1681 gttgcctaga tatctgataa gagttcttgt gacgttgtcc atccaggtag ccgataaaat
     1741 gaatatgaag ccctttgtct ggtgtatatt ccattaccca gtaatatccc gccaacatcg
     1801 tctgggtttc gctaagtaaa cggtatattt ccatacacat actgtgctta caggaatgcc
     1861 cgaaactggg cgtgtctttc ctgtaagcaa aatcaattct gaaaggtaac agtttagaaa
     1921 aacgttgaaa cataccatcc atgtgttcat tcacgtcttt cagaatcatg aaatccattt
     1981 tgtaattggg attggcatta tacattttat agtccttact ttataaaact cacaggaaga
     2041 gagaattacg taaatactga gagtagagaa acgccgtacc acggcattaa taaacgaagt
     2101 aaaccagcag cacagacaac ctgataaaac tgttttgtat taccgcatta ctgtgatgag
     2161 gtaatacttc agaatacagt gagactctct taaactgaga accagtacga attacaatgg
     2221 aaaaacacac ctcatagaat tgagtttcag gaagcaccac taaattcttt acaggaagta
     2281 atctgacatt cacaaaactc agtatcggct agctacaggc aacatcgcta ttaatattgt
     2341 cgaaccatta agtaaagccg agagagacca cgggtattta atcattagca taaaagaata
     2401 atggtataca gaacataccc acacaccata agaagattgt tccatacaga aaagatcata
     2461 tttacctata cctataaact gtaacggagt cataacacgc tctaatagtg ttccgatacg
     2521 taaccagtgt taagtacgta caggacaaca ttacacacat aattcaccat acagcttccc
     2581 ccatatgctt cactgcgtag tgtatggaga tatatgcatt acacacatac agaatactta
     2641 gcataaagag gagtgacatt accacgtaaa gagataagtt atacaacaaa catacaaaca
     2701 atacggcaca gacacacaat accataactc caactaataa tactaattaa ccttaattat
     2761 ttatggtata tgaaataaat gtgattctcc cggaactaac taacctaacc agacagatga
     2821 tcactgtata aaaacttcag ttaatactca tttgttttgt cgactcagta tataatgagt
     2881 gaccagtaat caggataatc acacttacat aacctgtaaa aaagaacaag aagaataagc
     2941 cactaacata atgcaaaatg gcatctgaat cgatcgcagt ttggcattcg aatcgatcat
     3001 gatttggccc aatccgtgat cgaatagagc ggaatacgca acataattca ccagacaaga
     3061 gcttacaccg aattacagat tatgtattaa tgtcaaaact ggcatatttt acagatacaa
     3121 tgaacatgca acccatcaat ctaatagggg cggtatgtac atctgcccat gaatgggatg
     3181 gcgctaagaa tacaaaacaa ttagacatag atcacaaaaa cacaccattc taattgaaaa
     3241 acaaaacaaa caaataaatc atactattat agaattttct cttgccagac atagataata
     3301 cccctgctaa ttttgctaat ggttagcaaa attagcaggg tatataaaga ttattgaccc
     3361 aaaacacact cagatgaatt cacgacagct caggagggtt attcctgatg tcatcttcac
     3421 aaataatggt taacgcatca tatatcattc tgcgtctgtt cctgttttcg gccttatcgc
     3481 catacaacat catcacattt ttaatttcat cgaccggtga tttcacatag tgaactaata
     3541 caccaagctt ctcaacacat tgcagtaaag tatatttctt actgcctgtg aaaagcccta
     3601 tcatagtttc tttacgttcg cgactggata atactttgtt ttccataatg acttctgcca
     3661 gcgtcttacc atcataaaca tactctttat ctatcagaat atttatctta tccagcacct
     3721 tctctacgtc atcaagcaaa cctgtaatat atgggggcgc aatatatggt ttcgtactga
     3781 tcttttcaag catacaatta tacatcttaa taacacgcat cagggtaaaa ttgttcgcat
     3841 catactgtat ttcaccctcc ttatatacag gatcaataag tggtattttt tcaataaatg
     3901 catcctgata ggcttttaca tggtcaagcc aataaaacaa cgagccatac tttatatttt
     3961 ttctatttac tttaacctcc agtgctgtcc gaagcaacga taatgccccc ctgacatatc
     4021 ctgaaggtaa ttcaccaaac aatccttttc tttccatgtc atcataatgc tgcagcacat
     4081 cattaatttt atttttcctt atcagacaaa tgtacaccgc aagtgtttcg tagcagctgg
     4141 tcacatgaac agcctcattc tgcaactgcc caaaatagct tataatcttt ttcagatatg
     4201 cgtcatcaca accattctta tcattaatat taaatacaag atctttcaga cgggaaatat
     4261 attcctgaga tttatcatca agtgaaacac tataacgaga caatgttcct tctgcactgt
     4321 tttgaaactt ataaaacatt tccagtaatt tttttgcata aggcgttgta tcaaagctgg
     4381 taaagtcata aagatattgc gcagcgtaac catacaaacc atgaatccag agtccttctc
     4441 tcaccaacac tctgagataa tatgcaaggc tttgaagtat cttcgtcttt cgttgtatat
     4501 caagtaagac acttaatgaa taaaatgcat tatagaattt atcaatattc ctgagcactt
     4561 tctccagttc atccttatcc agcttcttct cccggtaatc ctttttatat tcatttttga
     4621 tagccatgat atattgttgc atcaggctct tgcttttccc gttatccttc cgtatagttc
     4681 ctgtatattt ccccagagca tacagctttc cattttcatg aaaatcttcc ttgttgaatc
     4741 ccagttcagc cagatattcc cgaagataac gtttaacagc agcttcatcg ctttctgtat
     4801 cagcgtgatc aagacaagga caatacgatt tctgtaaaaa attataaata tgataataaa
     4861 aagcagagtc attctctggt ctgtatgtat tatacatatc tacaacaaga aaaataaccc
     4921 caaaagtgcg cagacaatag ctgcgaagga attcaggttt tagtgtggaa tgcactggat
     4981 aagaattcat catgtaataa aaatgttcat atcgggcata tatgatccgc cgttgctcct
     5041 gactgaacac cccttcagca tcaataatac gactgatctc attataacgt tgatcccaga
     5101 atttttgatg catttcaaaa ttggtactct tccgcttgtg tgccggattc tcgccttcct
     5161 taaacaaccc caaaacagta caaatatcat acaacatatt tgtaaatgtg cgcatatgct
     5221 gtgctctccg gttggtttgc attttatcgc agaggacatt ctgagcttaa cgtatcaaac
     5281 tgacacaacg acatgcaatg catatggaga acaatatgtc aaatcaaacc ggacagcttt
     5341 acacgccttc acgcatcctg cgtatggctg aacttacccg actgctgggt atatcaagat
     5401 ctactattta cgtgaaaata aatccggcct cgaaatacca cgaccctacc tttcccaaac
     5461 caataaggct tggtagtgca tccgtaggct ggcgggcatc tgccatcgat gagtggctga
     5521 tgttacatac ggcaccagcc tggtctgaac ctgaaaacaa actaaaggaa cttaatgatg
     5581 acgacaaaac tatcactccc tgaacttgac acaactaaac tgcctgacag gtgccaggca
     5641 ttacttgatg aaatgcatga agaaacagga atacgccgtg aaatacttct gtctgttatg
     5701 ctgacggtca tggctgcctc ggtccaggat acgcatgaag ttgaactttc cggagggcaa
     5761 cgtaccagcc ttcagatatt tatgtgtctt tcatcagcat caggcagtgg aaaaacctct
     5821 gcctgcgcaa aattaatcgc tcctgttcat gaaacagaag aagaactaca tcaggcatat
     5881 atcgacgata aaaaaaacta tgaccgaatg atggaaatgt ggacaacaga taaaaaaatc
     5941 ctggagcgga gatataaaaa ggaaattgaa agatccccgg aaaatgctgc tgctgcacga
     6001 gcagcacttg aaaagtgtat agcaaataaa cccgaacctc ccgtacaaca ggttctcatc
     6061 gtgaatgacg cgacaccaga agggatagct ttgaaactca gccagtcacc ctctcttctg
     6121 ttgctgtctg atgaaggagg tactattctg gataagcgtt tcgaacgcaa atcggcactg
     6181 tacaacacat tatggagtgg gcaaccagtc aacgttgaac gggcatccag acccgggttc
     6241 cggctgaatg atgtccgtct cactatactt atactgactc aaccggtaat atttaataag
     6301 ttttttactc tcactggcga tcagatccgg ggcaatggct ttctggcccg gttgctattt
     6361 tgcgaacccg gagcaattaa aataatgacg acagaaccgc atactgccac tcctgttgtg
     6421 acgcaacaat gtgcaagctg ttttcgtaag aaaagttttg gcagtcagat cagagactct
     6481 ctgagagcat ccagagaaag acgtgcaaaa agtaaagagc gcatctgcat gacattatca
     6541 aatgcagcat cccgcgcact ggagcgcttt cacaaagaaa atatggatac tgtcagacag
     6601 aatcctcata tgggtacatt tgaagacatt attgtcagaa aaagagagca ggttgtcagg
     6661 atagcagcac ttctggaact ggagaaagat ccagactcga cagtaataac gctggaaagt
     6721 actaatagcg ccatttatct cattgaattt tatttcaagc atcttatata taaacttgaa
     6781 tcccttcggg aaatatcccc ggctgagaaa cttgataaat ggcttcagaa aagaattatc
     6841 acaacagcag gttacatatt ccagaaaagt tatattttac aatatgcccc atatgctctc
     6901 aggaaaaaat gtgttctgga tgaagcactt gacatactgg cggaacaaag gaaaataagg
     6961 attgatgata acctggttgt ttatatagga aacacgatta caccatccga actggcaaaa
     7021 aaactaaata ttcctgcatt cgatgccgga gttttcattt gtgatcatca aaacatactg
     7081 aagtatcacc gaaacagact atagatacta tcggacaaaa aataaattat cacaagtgta
     7141 ttattcgtta taataatgaa taatcgttat ctgagtgcaa tgccaggttt tcattacgaa
     7201 ctggcattgc atttattttt caaaaaagaa agcagtaacc atttggctac tctgagtatg
     7261 ccagtccatg aaactaagcg aattccttag tgtttttagc aagttgagac atcacataat
     7321 ccgcccatgc ctgcatcatc ggtacacgct gctccagtag atcagtacga tgatatgccg
     7381 cctcaacctt atttttcagc gtatgagcga gcgccctttc cgccagatcc cgcgaatacc
     7441 cctgttcact acaccagtcc ctgaatgttg agcgaaaacc atgtgccgtg gcaactcgcc
     7501 ccggaatgtc actgacggct ttctttttac gcagaaaact tgtcaacacc atatcggaaa
     7561 ggatctgctg ctttctgggt gaagggaaca ccagttcatc atgcaggcca cgtatatttt
     7621 ccagaatgta aatagcctgc cgggataaag gaacacgatg ctgtagccta gctttcattc
     7681 tttctgcagg tatagtccat acccgcttat gaaaatcaat ttcagcccag cgcattcccc
     7741 tggcttcgcc cgagcgagtt gctgtaagta tcaccattaa taacagtgcg cgggtaacat
     7801 tataaggttc atcactatac acactggtcg ccacaaaaag cggtaactgc ctccagggca
     7861 ttgcgggttg gtgttcatca cgtcctcttg tctgctgagg aagcaaatgg tcaaccacat
     7921 caacaggatt tgccacacaa aaaccgtgcg cccatcccca ctgcataaca acatgaatgc
     7981 gctgtttaac ccggcttgcc gtttctgaca aggttaacca gactggacgc agtgtttctg
     8041 ccacgtccgc agccgtaatc gaatccagcg tttttgctcc cagttgagga aacgcgtaat
     8101 tctcaagcgt cgataaccac tgccttacat gctttggatt ctcccatcca ggagacagtt
     8161 ctgcatgtac acgcctggct gcatcggcaa atgttgggat aacgactttc tcagattcag
     8221 cctttttaat ctccagagga tcatcacctg cagcaagttg ctctcgcatt atccgggcag
     8281 tacgtgcagc ttcagcaata ctgacctctg ggtaagttcc caatccagca ttacgtcttt
     8341 tttgtgtcac cggacttaca taacgaaaaa cccatttccc ccgccccttt actgaagaag
     8401 gatgaagggt cagtccggta attcccccat ggggcaatgg tttgtcatca ggtttgatat
     8461 gtcttgcttt cgtatccgtc aatactgcca tacgctaatt cccgtcactc tggtatgcca
     8521 tccagtatgc cattagtccc tcgcttcact aagaatacat tagataacat cggacaacaa
     8581 aaagtgtaat acattgttta taaagaaatt atataacacc cctggattaa gtcagattta
     8641 tttcaggcgg
//
LOCUS       DAJVOY010000166         8624 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVOY010000166 DAJVOY010000000
VERSION     DAJVOY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.166"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..66
                     /locus_tag="OQL90_003372"
     CDS             <1..66
                     /locus_tag="OQL90_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCS1370033.1"
                     /translation="RYQAKYVSAIWQKLIPSQADF"
     gene            125..328
                     /locus_tag="OQL90_003373"
     CDS             125..328
                     /locus_tag="OQL90_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCS1370034.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            328..1353
                     /locus_tag="OQL90_003374"
                     /pseudo
     CDS             328..1353
                     /locus_tag="OQL90_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1381..1470)
                     /locus_tag="OQL90_003375"
     tRNA            complement(1381..1470)
                     /locus_tag="OQL90_003375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1434..1436),aa:Ser,seq:cga)
     gene            1564..2361
                     /gene="mtfA"
                     /locus_tag="OQL90_003376"
     CDS             1564..2361
                     /gene="mtfA"
                     /locus_tag="OQL90_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1370035.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2462..2537
                     /locus_tag="OQL90_003377"
     tRNA            2462..2537
                     /locus_tag="OQL90_003377"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2495..2497,aa:Asn,seq:gtt)
     gene            2851..>8624
                     /locus_tag="OQL90_003378"
     CDS             2851..>8624
                     /locus_tag="OQL90_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1370036.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYILGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFNNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPSWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLKALNTNENGIAEVTLKGTVLGVYTAEAILLNGKSDTKIVNIVPDTSNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGMHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTKPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLPNGNGYTTTVNIAPDTSNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNGVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGETTSLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQLTATLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTAS
                     MTGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDI"
BASE COUNT         2468 a         2067 c         2102 g         1987 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagctatc tggcaaaaac tgatcccgag tcaggctgat
       61 ttttaatgtt caaccctaat tgccgctaac cgtatatagt tagcggcggt tatgagatat
      121 agctatgagc agcttatttt taaccgaaga tgaattgcta atattaacgg gctgcaaata
      181 tgcaagccac cagcgaaaat ggttaatgga aaacgagctt ccgttctata ccaatcgtag
      241 tggcaaaccg attgtcagcc gggatctatt tacctgcaat aaaactttac caccacgcga
      301 ggtagagccg aattttggtg cgatctgatg ggaagacgaa ggaaaaatcc tgagcacgaa
      361 aaattacctc caaatgtata cccaaataaa tatagttatg tatggaaacc aacatccagc
      421 gaatctgtaa cactaaccgc catcaaggat ggtttagctg ctttatggaa aaagtatgag
      481 gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc gcttgtggga aaaattcctc
      541 gccagcgcct attacagtga cctcagtcca agaacacaaa aagattatct gcaacatcaa
      601 aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca gaacacatcc
      661 gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat gaagtcccgc
      721 gtttacagtt gggggtatga gcgagggtac gtgaaggcta acccatgtgc aggtgtaagt
      781 aaattcaagg ccaaaaaccg cgaacgatat gtaaccgaca aagaatacca ggcagtatta
      841 agcgttgcac ctcttcctgt ttttatcgca atggaaattg cctatctgtg tgcagcgagg
      901 gtttccgatg tgttatcgct gaaatgggag cagattggaa acgacgggat ctttatccag
      961 caagggaaaa caggaaaaaa acagataaaa gcatggagtc cacgattaca ggcggcgatc
     1021 gaaaaagcaa aacagttacc aacatccgcc tatgtaatca gcaatcaata cggcaaccga
     1081 tatatgtaca aaggctttaa cgaaatgtgg gtagaagcaa gaaatcgcgc aggcaaaatt
     1141 tcaggtattt taaccgactt cacctttcat gatctgaagg cgaaaggaat ttcagactat
     1201 gaaggaagca gtcgggataa gcaacttttc tctggtcaca aaaccgaggg gcaagtgcta
     1261 atctatgaca ggaaggttaa agtttcaccg acacttgatg tcccgttacc tgaaaatatt
     1321 ccaagtaatt ccacgtgtga tttttgtcac tgacttaatg atgtataagt gattgaattt
     1381 tggcggagag agggggattt gaacccccgg tggagttgcc cccactccgg ttttcgagac
     1441 cggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat gccaggaaat
     1501 ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa
     1561 acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc ccttccctgg
     1621 caggaagcac tatcgatccc ccttttaacg tgtctgacgg aacaggaaca aagcaaatta
     1681 gtcactcttg ccgaacgttt tttacagcaa aagcggcttg ttcctttaca gggctttgaa
     1741 ctggattcat taagaagctg ccggatagca cttctatttt gcctacccgt tctggagtta
     1801 ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc
     1861 gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt tcagtcaggt
     1921 cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga ttcttttgat
     1981 gcttctggtt ttaacctgat tattcatgaa gttgctcata agctggacac ccgtaacggc
     2041 gatcgcgcca gcggagttcc ctttattccg ttgcgtgagg ttgctggctg ggaacacgat
     2101 cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tggttggcga gaatgcggcg
     2161 agcattgatg cttatgcagc cagcgatcct gctgaatgtt ttgccgtact ttctgaatat
     2221 ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca acgtttctgc
     2281 caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac agactcgttt
     2341 tcggcgacga atgttcatta attaacaact ttgcatatta attaatcaat tgaaatgact
     2401 tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc gttcacacga
     2461 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     2521 tccagtcaga ggagccaaat tctaaaaatt cgctttttta gcgcaatgtc actgacctta
     2581 gttgaacatt gttttttaac ggatagcggg tttttaacat cttaagcgcc ctcgaccttt
     2641 atggttgagg gcgttttgct atgaacgcca tcaccatttt cccctcgatt ataaaacttg
     2701 agttattcag tagtctcccc tcttgcaact cacacccaaa actgcctaac gaaaagttat
     2761 taattttcaa tcatattgct atcagtattt acattttttc gctgtgctag aaagggcgca
     2821 tttatgttag ctcgttcagg gaaggtaagc atggctacga agaagagaag tggagaagaa
     2881 ataaatgacc gacaaatatt atgcgggatg ggaattaaac tacgccgctt aactgcgggt
     2941 atctgtctga taactcaact tgcgttccct atggctgcgg cagcacaagg tgtggtaaac
     3001 gccgcaaccc aacaaccagt tcctgcacaa attgccattg caaatgccaa tacggtgccc
     3061 tacatccttg gagcgctgga atcggcccaa agcgttgccg aacgtttcgg tatttcggtg
     3121 gctgagttac gcaaactcaa ccagtttcgt acgtttgctc gaggttttaa taatgtccgc
     3181 cagggtgatg aactggatgt cccggcacaa gttagtgaaa aaaaattaac cccgccgccg
     3241 ggtaatagca gtgacaacct cgagcaacag atagccagta cttcacagca aatcgggtct
     3301 ctgctcgccg aagatatgaa cagcgagcaa gcggcaaata tggcgcgtgg atgggcctct
     3361 tctcaggctt caggcgcaat gacagactgg ttaagccgct tcggtaccgc aagaatcacg
     3421 ctgggcgtgg atgaagattt tagcctgaag aactcccagt tcgattttct ccatccgtgg
     3481 tatgaaacgc ctgataatct ctttttcagt cagcatactc tccatcgtac tgacgagcgt
     3541 acgcagatta acaacggctt aggttggcgt catttcactc ccacatggat gtcaggcatc
     3601 aacttctttt tcgaccacga tcttagccgt taccactccc gcgccggcat tggcgcggag
     3661 tactggcgcg actatctaaa attaagcagt aacggctatt tgcgactgac caactggcgc
     3721 agcgcacctg aactggacaa cgattatgaa gcacgcccgg ccaatggctg ggatgtacgc
     3781 gcagaaagct ggctaccctc ctggccgcac cttggcggta aactggtcta tgaacagtat
     3841 tatggcgatg aagtggccct gttcgataaa gacgatcggc aaagtaatcc tcatgccata
     3901 accgctggac ttaactatac ccccttcccg ctgatgacct tcagcgcgga gcaacgccag
     3961 ggtaaacagg gcgaaaatga cacccgtttt gccgtcgatt ttacctggca acctggcagc
     4021 gcaatgcaga aacagcttga cccgaatgaa gtcgctgcac ggcgtagcct tgcaggcagc
     4081 cgttatgatc tggtggatcg caacaacaat atcgttctgg aatatcgcaa aaaagaactg
     4141 gttcgcctga ccctgacaga ccccgtgaca gggaagtcag gagaagtgaa atcactggtt
     4201 tcgtcgctac aaaccaaata tgccctgaaa ggctataacg tcgaagccac cgcactggaa
     4261 gctgccggtg gcaaagtggt cacaacgggt aaagatattc tggttaccct gccggcttac
     4321 cggttcacca gtacgccaga aaccgataac acctggccga ttgaagtcac cgccgaagat
     4381 gtcaaaggca atttgtcgaa tcgtgaacag agcatggtgg tcgttcaggc acctacgcta
     4441 agccagaaag attcctcggt atcgttaagt acccaaacat tgaacgcgga ttcccattca
     4501 accgccacac tgacttttat tgcgcatgat gcagcaggta atcctgttgt cgggctggtg
     4561 ctctcgacgc gtcacgaagg tgttcaggac atcacccttt ctgactggaa agataatggt
     4621 gacggaagct atacccagat cctgaccaca ggagcgatgt ctggcacgct gacgctgatg
     4681 ccacagctga atggtgtgga tgcggctaaa gcccccgccg tggtgaatat catttctgtt
     4741 tcgtcatccc gaactcactc gtcaattaag attgataagg accgttatct ctccggcaat
     4801 cctatcgagg tgacggtaga actgagagat gaaaatgaca aacctgttaa ggaacaaaaa
     4861 cagcaactga ataacgcagt cagcatcgac aacgtgaaac caggagtcac tacagactgg
     4921 aaagaaaccg cagatggcgt ctataaggcg acctataccg cctataccaa aggcagtgga
     4981 cttactgcga agctattaat gcaaaactgg aatgaagatt tgcataccgc tggatttatc
     5041 atcgacgcca acccgcagtc agcgaaaatt gcgacattat ctgccagcaa taatggtgtg
     5101 ctcgccaatg agaatgcagc aaacaccgtc tcggtcaatg tcgctgatga aggaagcaac
     5161 ccaatcaatg atcataccgt cacgtttgcg gtattaagcg gatcggcaac ttccttcaac
     5221 aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga cttttgatct gaaaagtagt
     5281 aagcaggaag acaacacggt tgaagtcacc cttgaaaatg gcgtgaaaca aacgttaatc
     5341 gtcagttttg tcggcgactc gagtaccgcg caggttgatc tgcagaagtc gaaaaatgaa
     5401 gtggttgctg acggcaatga cagcgccaca atgaccgcga ccgtccggga tgcaaaaggc
     5461 aacctgctca atgacgtcat ggtcactttc aatgttaatt cagcagaggc gaaactgagc
     5521 caaaccgaag tgaatagcca cgacgggatc gccacagcta cgctgaccag tttgaaaaat
     5581 ggtgattata gggttacggc ctctgtgagc tctggttccc aggctaatca acaggtgaat
     5641 tttatcggtg atcaaagtac tgctgccctg accctcagtg tgccttcagg tgatatcacc
     5701 gtcaccaaca cagctccgca acatatgact gcaaccttgc aggataaaaa tggcaaccca
     5761 ctaaaagata aagaaatcac cttctctgta ccaaacgacg tcgcaagtcg gttctcgatt
     5821 agcaacagcg gaaaaggcat gacggatagc aacggggttg caatcgcctc cctgaccggc
     5881 acgttagcgg gcacgcatat gatcacggct cgtctggcta acagcaatgt cagcgataca
     5941 cagccaatga cgtttgtggc ggataaagac agagcggttg tcgttctgca aacatcgaaa
     6001 gcggaaatca ttgggaatgg cgtggatgag acaactctga cagcaacagt gaaagatcct
     6061 tttgataacg tggttaaaaa tctgtcagta gtcttccgca cctcccccgc agacacgcaa
     6121 ctgagtctga aggcgcttaa tactaatgag aacggtattg ctgaagttac ccttaagggc
     6181 acggttttgg gtgtttatac agccgaagcc atactgctta acggtaaaag cgatacgaaa
     6241 atcgtcaata ttgtgcccga taccagcaac gcgcaggtca ccctgaacat cccagctcaa
     6301 caggtggtga cgaataacag cgacagcgtg cagctgacgg cgacggtgaa agatccgtcg
     6361 aatcatccgg tggcgggaat aacggtgaac ttcaccatgc ctcaggacgt ggcggcaaac
     6421 tttacccttg aaaataacgg tattgccatc actcaggcca atggcgaagc gcatgtcacc
     6481 ctcaaaggta aaaaagcggg catgcatacg gttaccgcaa cgttgggtaa caataatgcc
     6541 agcgatgcgc aacctgtcac attcgtggcg gataaggaca gcgcagttgt cgttctgcaa
     6601 acatcgaaag cggaaatcat tgggaatggc gtggatgaga cgactctaac agcaacagtg
     6661 aaagatcctt ttgataacgc agtaaaagat ctacaggtta ccttcagtac caagccggca
     6721 gatactcaac ttagtcagag cacaagcaat actaacgaca gtggtgttgc tgaagttacc
     6781 cttaagggca cggttttggg cgttcataca gtcgaagcca cacttcctaa cggcaacggc
     6841 tatacaacga cagtcaatat tgcgcccgat accagcaacg cgcaggttac gctgaacatc
     6901 cctgctcaac aggtggtgac gaataacagt gacagcgtgc agcttacggc gatggtgaaa
     6961 gatccgtcga atcatccggt ggcggggata acggtcaact tcaccatgcc acaggacgtt
     7021 gcggcaaact ttaccctcga aaataacggc attgccatca cccaagccaa tggggaagcg
     7081 catgtcacgc tgaaaggtaa aaaagcgggt acgcatacgg ttaccgcaac gctgggtaat
     7141 aacaatacca gtgattcgca gccggtaacg tttgtggcgg acaaaacctc ggctcaggtt
     7201 gtcctgcaga tgtcaaaaga tgagatcaca ggtaatggcg tcgataacgc aacgctaact
     7261 gcaacggtta aagatcagtt cgacaatggg gtgaataatc ttccggtaac attcagctca
     7321 gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc tggcatcgcg
     7381 caggccactc tcgcaggcgt tgcctttggt gagaagacgg ttactgcatc actggctaat
     7441 aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc ggcaaaaatt
     7501 atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa cagctccggc
     7561 agcgtcatca ccgccacagt cgttgataat aatggctttc cggtgaaagg tgtgactgtg
     7621 aacttcacca gcaacgcagc gacagccgaa atgacgaacg gcggtcaagc cgtgacgaac
     7681 gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga atcaggagcg
     7741 agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag cacatcaatt
     7801 aatgtcaacg ctgatgcgtc tacggcacat ctcaccttgc tacaggcact ttttgataca
     7861 gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta cggcaacggt
     7921 gtcccccagc aggaggtaac cctcagcgtt tcaccaagtg aaggcgtgac ccccagtaat
     7981 aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac cgctacaaaa
     8041 gccggggttt atcaattgac ggcaaccctc gaaaatggcg attcgatgca acaaacagtg
     8101 acctatgtgc cgaacgtcgc gaatgctgaa atcacgctgg cagcctcgaa ggatccggtg
     8161 attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac agagggcaat
     8221 gcgatagcca acactgaggt aacatttact ctgccggaag atgtgaaggc gaacttcacg
     8281 ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt cacgctgaaa
     8341 ggtacaaaag caggcgctca tactgttaca gcatcgatga ctggcggtaa gagtgagcag
     8401 ttggtggtga actttattgc ggatacgctc actgcgcagg ttaatcttaa cgttaccgag
     8461 gacaatttta tcgctaataa cgtcgggatg accaggctgc aggcaacagt gactgatgga
     8521 aacggcaacc cgttagccaa tgaggcggtg acattcacgc taccggcaga tgtgagcgca
     8581 agctttactc tcggacaagg cggttccgcc attactgata tcaa
//
LOCUS       DAJVOY010000167         8612 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVOY010000167 DAJVOY010000000
VERSION     DAJVOY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.167"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..190
                     /locus_tag="OQL90_003379"
     CDS             <1..190
                     /locus_tag="OQL90_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370037.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OQL90_003380"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OQL90_003380"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1370038.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OQL90_003381"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OQL90_003381"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1370039.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OQL90_003382"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OQL90_003382"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1370040.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OQL90_003383"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OQL90_003383"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1370041.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OQL90_003384"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OQL90_003384"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1370042.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OQL90_003385"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OQL90_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1370043.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OQL90_003386"
     CDS             7144..7353
                     /locus_tag="OQL90_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1370044.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OQL90_003387"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OQL90_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1370045.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..8612
                     /locus_tag="OQL90_003388"
                     /pseudo
     CDS             8228..8612
                     /locus_tag="OQL90_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT         2029 a         2046 c         2309 g         2228 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcattt cgcgattaat ag
//
LOCUS       DAJVOY010000168         8609 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVOY010000168 DAJVOY010000000
VERSION     DAJVOY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.168"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(198..1521)
                     /locus_tag="OQL90_003389"
                     /pseudo
     CDS             complement(198..1521)
                     /locus_tag="OQL90_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1521..3137)
                     /locus_tag="OQL90_003390"
     CDS             complement(1521..3137)
                     /locus_tag="OQL90_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1370046.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3433..4149
                     /locus_tag="OQL90_003391"
     CDS             3433..4149
                     /locus_tag="OQL90_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1370047.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4146..5816)
                     /locus_tag="OQL90_003392"
     CDS             complement(4146..5816)
                     /locus_tag="OQL90_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1370048.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGSIGKLYWFMPPSANLALRELGIVLFLSVVG
                     LKSGGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMT
                     DPPALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6012..6440)
                     /gene="ibpB"
                     /locus_tag="OQL90_003393"
     CDS             complement(6012..6440)
                     /gene="ibpB"
                     /locus_tag="OQL90_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1370049.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6552..6965)
                     /gene="ibpA"
                     /locus_tag="OQL90_003394"
     CDS             complement(6552..6965)
                     /gene="ibpA"
                     /locus_tag="OQL90_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1370050.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(6959..7031)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7271..7603
                     /locus_tag="OQL90_003395"
     CDS             7271..7603
                     /locus_tag="OQL90_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1370051.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7605..>8609)
                     /locus_tag="OQL90_003396"
     CDS             complement(7605..>8609)
                     /locus_tag="OQL90_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1370052.1"
                     /translation="PKSEKYVFIHGPENPDETWYYDFHHRRGVIAEGGKVSNLDAMDI
                     TAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNVGVAAPFGPVNVQ
                     KQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGDTLSPKGEKV
                     PELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRAYPGLVNVPR
                     HWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAFNWHPSGEWL
                     GFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWMEGGQLWITETDR
                     "
BASE COUNT         2187 a         2286 c         2161 g         1975 t
ORIGIN      
        1 tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac agcaaacacc
       61 tggttaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt ttacggcgca
      121 ccggataaat ttggttatgc gccgtcagcc gtgctaaaga atacggacca gcacccaacg
      181 gatgccagtc cacacaatta acgtaactct ggccagtacg ctttgttggc ctccagcaac
      241 tcatccagaa tagctttggc gaccgaggcg ctcggtaccg ttttcgataa cgtaatcgcc
      301 tgccacaggt gctgatatga acgctgttcc caggcgtcca cgaccagttt ttccaccgcc
      361 acttgctgac tcattaaccc tttctgaaac tgcgggatat cgccgaccac cagtggttct
      421 ggtccgttgt gcccgaccag acacgggatc tcgaccatcg cttcatcatc aaagttatgg
      481 attgccccgt tgttaggcac aatcagcaac atccgctcct gagtgttgaa ggcaattgcc
      541 gccgccagat cgacgatgta tgacgcatgt tcgtcaatct ccagcttggc cgccgctgaa
      601 tttcctgccg ctgtaatggc gcggcaagca tcgaaaacct gtttctcgcg atgttccatt
      661 cacctcattc gcgcgggtat gttcagggtt ggaatgctga acaacataat ccgggaagag
      721 ataatatttc agataggtgt tcggcaatgt atccgggtcc agtgcctgga catcccgcgc
      781 ttttgcatag gtgtcgttcc agctcgcttc agtatgtgta tcttgctgcg gtggaacata
      841 accgtgctta gagacatatt gccgcagctg gggcatcagg tcgttgccct gcaaatcttc
      901 aattgatgtc caccagccaa agtggttcag gccgtagtag cgcacgcgca tctgtttgcg
      961 atcttgcagc ccaacaattt gcgccatccg gctttcaata ccgattggca tgtcacagat
     1021 gttgaggatt ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa ttgctgccgg
     1081 gttggagtag ttgagcatcc aggcatttgg tgaatatttt tccatataat ccaccagttc
     1141 caggacgccg ccaatggaac gcatgccgta cgctattccg ccgggtccgc aagtttcctg
     1201 accaacaacg ccgtggcgca gcgggatttt ttcatccagt tcgcgcatcg ggtatttgcc
     1261 gacgcggatg tgcgccatga caaaatcaac gtcgctgaat gccacttcag gatcggtggt
     1321 gtaactaaac gcaatgtccg gcgctttctc tttaaggatg actttacagg cttcggcaat
     1381 cacttcctgc cgcgcaccat cgttatcata aaatttcagt gcacgaagcg ggaaacggtc
     1441 ctgattcgcc aggagcatca acacgatgcc tggcgtaaag gtgcttccac cgcctgcgac
     1501 aaccactgag aatttggtca ttatactgcc tccgtaatgg caacattttc tgctgattga
     1561 tgagaattaa ttaagctatc gagctgttca cgcagctggg atacatgcag accaatgatc
     1621 acctgaatgg catcgccact acggaagacg ccgtgcgctc ccagcttttt aaagacttcg
     1681 tcatccagcg tttgtgacat gtcatgcagt gcaatacgta aacgcgtcgc gcaattgtta
     1741 atgctggaga tattgccgac cccgcccagg gcttgcagga taccggcagc ctgatccagc
     1801 tctttttttg gctctgcagc ggtggtttgg cctcgcgagg ctttgtattc ggcttttgag
     1861 tagagtttca cttccgcatc ttcacgtccc ggcgtgcaca tgttgaattg cagaatcagt
     1921 gtgcggaaaa ccacgaagta cagcagggta aagcacaacc caatggcgat ttgggtcagc
     1981 atcatatccg cgtggttgct gaacatcggg atccagtttt gcggtaaaac ctggtcaatc
     2041 agacctccgc ccatgttgcc caccacacca aagagataca tcacggtcga cattgaggcc
     2101 gccagcacgg cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt aaattccagc
     2161 ggttcggtaa ttcccaccag catggcggtt aaggttgcgg gaatcagcaa gcccgccact
     2221 tttacccgat tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc cacggcacca
     2281 aagatttttg agttaccgtg cagggcaaaa ccgccttccg ggaacaacga tttcagcggc
     2341 tcggcgctca gactgaactc ttgcagatgc tgcgcccagt acatctgaat gccgccttca
     2401 acagctgccg gaccaaagat aaactgtccg tagatgaagt ggtgtaaacc ggttgggatc
     2461 agaatacgtt cgaggaaggt gtaaacccac cccccaagcg cacccgccga acgcaggaac
     2521 gcttgcagag attcaatccc catttgtact tttggccaac cgagcaacgt cagccaggca
     2581 caggggatca tcaccaggaa ggcgataatc accacataag acgtcccctg gaaaatgccg
     2641 agaaaaaccg gcagtttttt atcgaacaga cggttatgca gcgccgtcac aatgccggaa
     2701 atgataattg cgccgataat gctggtatcg agggttttaa tcccggccat cattgtcaga
     2761 ccgctacctg ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc ccagatcatt
     2821 cccatcgcgt tgatgaaata gttccaggtc aggaaactca ccatcaccgc cagacaagca
     2881 cgcccctgcg cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat cagcggcata
     2941 ttacggaata ccgtccaacc gccctcttca ataatgtgta cgatttgcgc gaataaactg
     3001 ttcggatcgg tcagtgattc cccgacaaac atcgggtttt gcagcaagat ggcaagaccc
     3061 accacaatcc cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt
     3121 tgaatttgac tgagcatttc cacattctct tttctcaatt ctgtaagtag gaagcctgat
     3181 tggttatgcg gcaggcctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc
     3241 caatgggatt cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc
     3301 gaaaaagtag acatctgtga gcactggtac acagcatcga acacatcttt aaaaaaaaga
     3361 tgttttttca atcgattaag cagaacttgt gggcgcatta cccgggcttg caggcaaaaa
     3421 agagatctag agatgatcta caaaagcatt gcggagcggt taagaattcg acttaattcc
     3481 gcggatttca cgctaaacag ccttcttccc ggtgaaaaaa agttggcgga agaatttgca
     3541 gtatcccgga tgaccatccg taaagcgatt gacctgctgg tagcgtgggg gctggtggtc
     3601 cgccgccacg gcagcggcac ttacctggtg cgcaaagatg tgctgcatca aaccgccagc
     3661 ctgaccggac tggtggaggt gttaaaacgg cagggaaaaa cggtcaccag ccaggtgctg
     3721 atttttgaaa tcatgcctgc gcctccggcc attgccagcc agttacggat tcaaatcaac
     3781 gagcagatct acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct gatgctggaa
     3841 gacagctata tgccggttaa actgttccgt aatctttcgc tgcaacatct ggaagggtcg
     3901 aagtttgaat atattgaaca agagtgtggg attttgattg gcggtaatta tgaaagcctg
     3961 acgccggtgc tcgccgatag actgctggcg cggcaaatga aggtagcgga acacacgcca
     4021 ctgctgcgga tcacctcgtt gtcatatagc gagagcgggg agtttttgaa ttattcagtg
     4081 atgttcagaa atgccagcga ataccaggtg gagtaccatt tacggcgact ccacccggaa
     4141 aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat aattcgcaga
     4201 aacatcacta acggatagac agtggcgtaa gagagcgccg gcgcaccact ggttggatga
     4261 agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag catcccgcac
     4321 atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt aatcagcgga
     4381 acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatt gaccagggta
     4441 ttcacaaaat ccccaccaga tttcagacca acgaccgaga ggaacagcac gatccccagc
     4501 tcccgcagcg cgaggttggc acttggcggc ataaaccagt acagcttgcc gatactgccg
     4561 atactgccga tacgcccgag gatcaacgcc ataatcagcg gaccgcccgc cagccccagt
     4621 ttcaacgcgg ccgggaaccc aggcacaaag acgggaatag aacctaacaa tacgccaagc
     4681 ccgatgccaa taaacactgg cagcatctga acctgttgca gtttttgctg cgcattcccc
     4741 agcacattgg caacggcatc aattgcggac ggacgcccca ccagattgag gatatcgccg
     4801 aactgcaggc tgatatcgcc actggcgacc agttcgaccc cggcacggtt caggcgcgag
     4861 ataacaacgt catagcgttc tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt
     4921 tcattggtga ccaccacacg ctcgacgcgc aaatcagtgc ctttcgtgga cagcgaagta
     4981 tcgacctcct gaccaatcac cagttgcgca ttatgtaaat ccgctggctg ccccaccaga
     5041 tgcagcaaat cgcccagttg gataatggta tctggcgaag gaacttttag ggtttcttcg
     5101 cgtttcagac gcgagcagat aattttgtcg ccgttgagaa tcggtacatc tttaatagct
     5161 aaatcatgca ggttagggtt ctcaacgcga atattgatag tcttgatcag cgcgccgcca
     5221 ttggtgcgtg aagactcgtg ctgctgagct tctgtctcga cattgacgcg gaaaataacc
     5281 cgcaacatcc acatggtgaa caaaatcccg caaatgccga atggatacgc catcgcgtaa
     5341 ctcatcccca tctgatcgac catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc
     5401 cctgccccca gcgctggcgt attggtaacc gcaccggaga aaatccccag cactaccggc
     5461 agtggaatat caaacagttt atgcaggatg gcggtaacca gaccaccgat gatgacgatc
     5521 agaacagcaa acaggttgag gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc
     5581 cctacttgaa tcccgatggt ataaacgaac aggatcaggc caaattcctg aataacatgc
     5641 agcatatcgc tacttaatgt catccccgcc tgagaaacaa aatggccgac gatgatccca
     5701 ccaaacagca cgccgccaat acctaatcct atgccgcgaa atttgacgtt gccgataaac
     5761 aaaccgacga ctgccaccaa agccagaata ctgaccgtta atgctatatc actcatcgtc
     5821 catttctctt gagataacat tttaattgct acggattatg gctgaatcgg tgaagcagat
     5881 ttgccttata gcgcacatta tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa
     5941 atttaacgca ttgttgaaga agaaagacaa aaaaagcccc accagaatgg cggggcaaag
     6001 agaatagcta gttagctatt taacgcggga cgttcgctga tagcgatacg ctgcgctgcg
     6061 atgggttcag gctcattacg aattaaatca atatgcagta aaccgtttac gaaggttgcg
     6121 ccagagactt ccatattttc agccagcgta aagctcaggc taaatggctg attcataagc
     6181 ccttgatgca gccatttttt ctcttctttt ggctgctccg gcgtgccttt tacgctcagg
     6241 cgcgtacctt ccagttgaat ctctaaatct tcctgacgga aacctgccag cgcaagggta
     6301 atgcggtagt ggttatcgtc gcttttctca atgttgtacg gcgggaagct ctggctttca
     6361 ccggcgtttt gcagtgcgtt ggccagtttg tcaaaaccga tccattgacg catcagtggg
     6421 gataaatcga agttacgcat agtcatttct ccttctaaga agcgagtaag tacctgcaaa
     6481 tccgaagatt cgcatatgct ccctgacggc gagcatggag atgtcaggcc gcgtcaggcg
     6541 gccttaggga attagttgat ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt
     6601 tcgagatcga tatacagcaa accatttacc aggttagccc cacgaacatg aatgttctca
     6661 gctaactgga atttgcgttc aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc
     6721 tctttttgtt cgtcggcgtg agcacctttc accaccagca gattatcctg ggcggtaatt
     6781 tccagttcgc tctcagcaaa accagccaca gcgatagcaa tgcggtaatg gttttcgtct
     6841 accagttcaa cgttatacgg agggtagccg ccattactct ggctctggtt gttttctaag
     6901 tggttaaaca aacggtcaaa tccaatagca gaacggtaaa gcggggataa atcaaagtta
     6961 cgcataatca atagctcctg aaatcagcga gaatgtaata ccttccacaa tggacaggtc
     7021 aggtagccag aacacccatc aggcgcgtcc tcatcggcta cgatgtaaaa atgggtctgg
     7081 aaatgaactt ttcaagcctt atcagcggac ttttttttgc agtttatggt ctattgcata
     7141 gactgagggg gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg acgtaatgat
     7201 gatgttattt tcagcaaggg atcgctataa ctcatcaggc caacacccac gatcaacaga
     7261 tgaatttatt atgataagaa atgtgttgtt agcgttcatg atatgcagcg gaatgacatt
     7321 actcggagga tgctccagcg tgatgtccca taccggaggg aaagaaggaa cgtatcccgg
     7381 cacccgcgcc agcgcaacca tgattggcga cgatgaaacc aactggggca ctaagtcgct
     7441 ggcgattctc gatatgccgt ttaccgctgt gatggacacg cttttactgc cgtgggatgt
     7501 gttccgcaaa gacagctcgg tgcgctcaca cgttgaaaaa agcgaagcca acgcgcaagc
     7561 caccaatgcg gttattccac cggccagaat gcctgataac tgatttagcg atcagtttcg
     7621 gtgatccaca gctggccgcc ttccatccac gccagccatt gaccatccgg cgagaagacc
     7681 acggcgtccg cagaaggtgg attggcgtgg ttttcggtta aatactcaac ctcgccactt
     7741 tgcgcatggg cacaagcaat tcgattatcc agcacaaagc ccaaccattc tcctgacgga
     7801 tgccagttaa atgcagactg aatatccgtt ttgttatggg ttaactggcg cggctcgccg
     7861 ccctgtggcg agataagcca cagttgcaca atgccgttat catcacgcat taaaaacgcg
     7921 atttgcgtac cctgcggatt acagcgcacc cagtggcgcg ggacgttgac taaccccgga
     7981 taagcccgat ggtgggtaaa ggttaaacgt cgctgcacga cgccgcgcgg tggcgcgggc
     8041 agggtggttt ccgttccact taacggcgca tcacctgccg ctttccagcc agcttcatct
     8101 tgcggtaact caacgataaa cagctccggc actttctcgc cctttggcga aagtgtgtcg
     8161 ccaataaacg ccagcgcgtg atttcctacc catccttctt cataagcacg attgatttca
     8221 tcgctgccag gctgcggcgt gggcgtggtt ttactcacca gcacgcacca gtggctaccg
     8281 ctgtattcac gcggatgctg cttttgtacg ttgaccgggc caaacggcgc agcaacaccg
     8341 acgtttcgca aatccagcgc cggatcgagt tcatgcatta catggtcgtt ataggtaaag
     8401 ctcacccttt caccgttcgg gctaaagaca tgcacatggc tgccgccgcg cagcgctcct
     8461 ggcgtatacg gcgcggtaat atccattgca tcgagattgc tcaccttgcc gccttcagca
     8521 atgactccgc gtcgatgatg gaaatcgtaa taccatgttt catcaggatt ttccgggcca
     8581 tggatgaaaa catatttctc tgacttcgg
//
LOCUS       DAJVOY010000169         8598 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVOY010000169 DAJVOY010000000
VERSION     DAJVOY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.169"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..183
                     /locus_tag="OQL90_003397"
     CDS             <1..183
                     /locus_tag="OQL90_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCS1370053.1"
                     /translation="EAIKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQ
                     KGEVVGVDDQCKEHKE"
     gene            402..644
                     /locus_tag="OQL90_003398"
     CDS             402..644
                     /locus_tag="OQL90_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370054.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OQL90_003399"
     CDS             complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OQL90_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1370055.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OQL90_003400"
     CDS             complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OQL90_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1370056.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OQL90_003401"
     CDS             complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OQL90_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1370057.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OQL90_003402"
     CDS             complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OQL90_003402"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5910..5912),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1370058.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6691..7524
                     /gene="fdhD"
                     /locus_tag="OQL90_003403"
     CDS             6691..7524
                     /gene="fdhD"
                     /locus_tag="OQL90_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1370059.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7678..>8598
                     /locus_tag="OQL90_003404"
     CDS             7678..>8598
                     /locus_tag="OQL90_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCS1370060.1"
                     /translation="MKRNLLSSAIIVAIMALSLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPY"
BASE COUNT         2050 a         2175 c         2272 g         2101 t
ORIGIN      
        1 gaggcaatta aacggctgaa caagcttcgg ggctggcgta aggtttcacg atctgcaatt
       61 ttacgcgaag cggttgagca atatctggag cggcagcaat ttccggtgcg taaagcaaag
      121 ggaggcaggc aaaaggggga ggtagttggt gttgatgatc agtgtaagga gcataaggaa
      181 tgaaaaaaat ttttcattct tgaatataaa aaaacagatg cccttattct ggtattaata
      241 caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcgtatc tggaaaacgc
      301 ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact
      361 tttctctcca tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta
      421 tagatatggg cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc
      481 tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc
      541 tgataaatca atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg
      601 aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt
      661 caatcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc
      721 cggcctacaa agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc
      781 agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt
      841 tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa
      901 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
      961 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
     1021 catagccttc ttgctccatt cgcgcgtcca gtaccagaga agcgaggtca tctgccacgg
     1081 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
     1141 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
     1201 tctgttcaca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc
     1261 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
     1321 aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct ttgccgggga
     1381 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
     1441 cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
     1501 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
     1561 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
     1621 gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca
     1681 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
     1741 gcaggtaatc atccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
     1801 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     1861 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     1921 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     1981 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     2041 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     2101 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     2161 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     2221 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     2281 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     2341 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     2401 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgctaggat
     2461 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     2521 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     2581 tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca
     2641 aagccaacag ctgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     2701 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     2761 ataacgtgtg taccaccgac gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg
     2821 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     2881 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     2941 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     3001 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     3061 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag acagcctgga
     3121 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     3181 acttccgaga agcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac
     3241 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     3301 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     3361 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga
     3421 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     3481 aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaaagtatt ggcaataaag
     3541 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     3601 ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag
     3661 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     3721 gactccccga tttccacaaa ctgctctggc tgcaaaattg cgttcaacag cgcgtgtttg
     3781 gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     3841 gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt cgagataacg
     3901 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     3961 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     4021 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     4081 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     4141 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     4201 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc
     4261 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     4321 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     4381 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     4441 tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc gccaccctgt
     4501 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc gtcagtcagc
     4561 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg
     4621 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     4681 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     4741 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     4801 ttgttcggga atgaggcaac agggttaaaa ccctggcaga tatagccatt gaccttgccc
     4861 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     4921 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     4981 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     5041 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     5101 cctggcaggc tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     5161 cgcagggcgt taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc
     5221 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     5281 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     5341 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     5401 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     5461 ccgttttcgt ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca
     5521 tagccggtga aaaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg
     5581 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     5641 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtt agctaccgcc
     5701 gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     5761 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     5821 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     5881 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     5941 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     6001 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     6061 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     6121 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     6181 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     6241 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     6301 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     6361 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca
     6421 aaacctagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     6481 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     6541 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     6601 cagaattggc tcgatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     6661 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa
     6721 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     6781 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     6841 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     6901 ctttccgagg ggattatcga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     6961 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     7021 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     7081 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     7141 gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact
     7201 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt
     7261 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     7321 caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     7381 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     7441 gagcgctgta atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc
     7501 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg
     7561 acttattcat ttcgtgaatt ttattatttt tatttataaa taaaacgcgt gtagtggcgt
     7621 tttctttttt catttattat gttcactctc ttatctatat atttataagg caattaaatg
     7681 aaaaggaatt tattatcttc cgctattata gtcgccatca tggccctcag tctgacgggt
     7741 tgtgatgata aaaaagccga aacagaaacg cccccgcctg ccaatagtca acctgccgca
     7801 ccagctcctg aagctcccgt tgcaaaagca gaagctaaac ctgaaacacc tgcgcaaccg
     7861 gtggtcgatg aacaagcggt tttcgacgaa aaaatggatg tctatatcaa gtgctataac
     7921 aagttacaga tccctgtaca gcgcagtctg gcgcgttatg ttgactggct gaaagatttt
     7981 aaacaggggc ctaccggtga agagcgtact gtttatggca tctacggcat tagtgaatcc
     8041 aacctcgctg agtgtgaaaa aggcgtaaaa agtgctgtgg cgttaacgcc tgcgctgcaa
     8101 ccaattgatg gcgtggcggt gagttatatc gatgctgccg tggcgttggg taataccatt
     8161 aacgaaatgg ataaatatta cacccaagaa aattataaag acgatgcgtt tgcgaaaggg
     8221 aaaacgctgc accagacatt cttaaaaaat ctggaagcct ttgaacctgt agcagaatct
     8281 tatcatgcgg cgattcagga aattaacgat aagcgccagc ttgcggaact gaaaaatatt
     8341 gaagaaagag aagggaaaat attccactac tattctctgg cagtcatgat ttcagcgaaa
     8401 caaattaata acctgatatc gcaagagaag tttgatgttg atgcggcaat gaagaaagtg
     8461 tctgaactgg aaacgctggt ggcgcaggcc aaagaagcgg ataaaggcgg catgaatttc
     8521 tcgtttatta attcggcagg ccagtatcaa ctcgaggcta aaaaatacgt tcgccgcatc
     8581 agagataaag tcccgtac
//
LOCUS       DAJVOY010000170         8563 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVOY010000170 DAJVOY010000000
VERSION     DAJVOY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.170"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..49
                     /gene="yobI"
                     /locus_tag="OQL90_003405"
     CDS             <1..49
                     /gene="yobI"
                     /locus_tag="OQL90_003405"
                     /inference="COORDINATES: protein motif:HMM:NF038179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1370061.1"
                     /translation="HFFTEYVIFKYLLPI"
     gene            188..799
                     /gene="ruvA"
                     /locus_tag="OQL90_003406"
     CDS             188..799
                     /gene="ruvA"
                     /locus_tag="OQL90_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1370062.1"
                     /translation="MIGGLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            808..1818
                     /gene="ruvB"
                     /locus_tag="OQL90_003407"
     CDS             808..1818
                     /gene="ruvB"
                     /locus_tag="OQL90_003407"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1370063.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1868..2653)
                     /gene="znuB"
                     /locus_tag="OQL90_003408"
     CDS             complement(1868..2653)
                     /gene="znuB"
                     /locus_tag="OQL90_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1370064.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2650..3405)
                     /gene="znuC"
                     /locus_tag="OQL90_003409"
     CDS             complement(2650..3405)
                     /gene="znuC"
                     /locus_tag="OQL90_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1370065.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3484..4416
                     /gene="znuA"
                     /locus_tag="OQL90_003410"
     CDS             3484..4416
                     /gene="znuA"
                     /locus_tag="OQL90_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1370066.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4432..5754
                     /gene="mepM"
                     /locus_tag="OQL90_003411"
     CDS             4432..5754
                     /gene="mepM"
                     /locus_tag="OQL90_003411"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1370067.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5874..6845
                     /gene="lpxM"
                     /locus_tag="OQL90_003412"
                     /gene_synonym="msbB"
     CDS             5874..6845
                     /gene="lpxM"
                     /locus_tag="OQL90_003412"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1370068.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(6934..>8563)
                     /locus_tag="OQL90_003413"
     CDS             complement(6934..>8563)
                     /locus_tag="OQL90_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1370069.1"
                     /translation="PHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTSESAT
                     KAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCISRVYL
                     ETEKEYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININNNWL
                     KARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSENGDLISLYDKRYGKEYIT
                     ENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYHYNS
                     SYLRFAFTLTQDSPLVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDRPTH
                     SDDSFAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLKLTLLRSQHKPGEV
                     VKTDNYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPVKPHP
                     GELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYHYVQY
                     VDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2317 a         1988 c         2106 g         2152 t
ORIGIN      
        1 gcacttcttc actgaatacg taatatttaa atatttgctt ccaatataac ctgtagaata
       61 aatcatactg cgccattttt cagttcatcg agacacctcg caagttttct tcatccttcg
      121 ctggatatct atccagcatt tttttaccat acagcattat ctttgattca ttacgcagga
      181 gcgtcatgtg ataggcggac tcagaggcat catcattgaa aaacaacccc cgctggtgtt
      241 aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt tttatgaact
      301 ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca
      361 actgctgtac ggttttaaca ataaacaaga gcgcacattg ttcaaagagt tgatcaaaac
      421 caacggcgtc ggcccgaagt tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt
      481 cgttaatgcc gttgaacgtg aagaagtggg ggcactggtg aaactgccgg gtattggcaa
      541 aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt tgcatggcga
      601 tctgtttacc cctgccgccg acctggtact cacatcacct gccagcccgg cgaccgacga
      661 tgctgaacag gaagcggttg ccgcgctggt ggcgctgggc tataaaccac aagaagcaag
      721 ccgcatggtg agcaaaatcg ctcgccctga cgccagcagt gaaacattaa ttcgcgaagc
      781 cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat ttctgccggt
      841 accactttgc cggaagatgt agcagatcgc gccattcgcc ctaaattact ggaagagtat
      901 gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc gaaactgcgc
      961 ggcgatgccc tcgatcattt gttgattttt ggtcctccgg ggttgggtaa aactacgctt
     1021 gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg tccggtgctg
     1081 gaaaaggcgg gcgatctggc agcgatgctc actaaccttg aaccgcatga cgtgctgttt
     1141 attgatgaga tccaccgttt atcgccagtt gttgaagaag tgctgtaccc ggcaatggaa
     1201 gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat taaaattgat
     1261 ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag gttcgctgac atcaccgttg
     1321 cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga tctgcaatat
     1381 atcgttagtc gcagtgcacg ctttatgggg cttgagatga gtgatgacgg cgcgctggaa
     1441 gttgctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg tcgagtgcgt
     1501 gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc tcaggcgctg
     1561 gacatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt gttgctggcg
     1621 gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc ggctattggc
     1681 gaagaacgtg aaaccattga ggatgtgctg gaaccttatt tgattcagca aggctttttg
     1741 cagcgtacac cgcgtgggcg tatggcgacg acgcgggcgt ggaatcactt tggcataacg
     1801 ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg catccgacaa atgtgttcag
     1861 cgatagatta gctggcctgc tttttcatca tactgataat aaataacagt gctgcacata
     1921 gcaccaccga cggacccgcc ggcgtatcgt aaaatgcgga aaaggttaaa ccgcccgtta
     1981 cggcaaccat ccccaccaac acagcgacac ctgccatctg ttccggcgtg cgggcaaagc
     2041 gacgtgcagt agcagcagga ataatcagca gtgaagtaat aatcaacgcg ccaacaaatt
     2101 tcatcgctac accaatcgtc aatgccgtca ccagcatcaa caacaatttc acgcgctgta
     2161 atttcacacc atcaacaaac gccagatccg ggctaatcgt catcgacagc aaattgcgcc
     2221 attgccagaa caaaatagcc accacgatga ccacgccaat cgcaatagcg atgagatctt
     2281 ctggcgtcac tgccagcaaa tcaccgaaca ggtaagccat caaatcaaca cgaatgttag
     2341 acatcagact aacgaccacc aggcccagcg acagggcact gtgcgccata atccctaata
     2401 acgtgtcaag cgccagctgt ggacgtttct ccagccatac cagaccgccc gccaggagca
     2461 gcgtaacggc aattaccgca tagaatggat tcacgtccag caacaaacca aacgcgacgc
     2521 caagtaatga ggcatgagcc agcgtatcac cgaaataaga catacgacgc cagactacaa
     2581 acgaacccag cggacccgcg gcacaggcga gcatgatccc ggctaaccaa ccgggaaata
     2641 ataattcaat catgagcgat catttccccg acgcaaaaca attcgtccct gtaaatcgtg
     2701 acgatgatta tgatgatggc gatagatacc cagttgttca gcaccacgag gaccaaacat
     2761 agaaataaac tccggatgta gggaaacaac ttccggtgtg ccggaacaac aaatgtggtg
     2821 attcagacac agcacttcat cggtttttgc cattaccaga tgcagatcgt gagagaccat
     2881 taaaacgcca caatccagtt cgcgacgcaa ctggtcaata aggtcatata acgccacctg
     2941 gccatttaca tccacgcctt gagtgggttc atccagcact aataattgcg gtcgatttaa
     3001 caatgctcgc gctaacagta cacgctgtgt ttcgccacca gagagctttt gcatcggtgc
     3061 gttaatcaga tgcccggcct ggacacgttt cagtgcaggc aaaatatctt ctttatgtgt
     3121 gccagggcgt aagcgtaaaa aacggtttac ggtcagtggc aacgtggtgt cgagatacag
     3181 cttctgcggt acatagccga tgcgtagttt tccgttgcgc ttgataaccc cttcatcggg
     3241 tgttaccagc ccgagcacta cccgtaccag tgtcgactta cctgcgccat ttggcccaag
     3301 taaagtcaaa atttttccag gtttaagttc cagcgacaca tcagagagga cgcggcgttg
     3361 gccaaaagaa accgagacat tttccaggga aaccagactt gtcatgttaa ttttagtctt
     3421 gcagtagtca tgagatgtta taatatcaca cttctcatat tcattacgat gattggtcgc
     3481 attatgttac ataaaaaaac gcttcttttc gcagcattat ccgccgctct ctggggaggt
     3541 gcaacacagg ccgcagatgc cgccgttgtc gcttcgctta aacccgttgg gttcatcgct
     3601 tctgccattg ctgatggtgt aacagaaacg gaggttttac tgcctgacgg cgcttcagaa
     3661 catgattatt cactgcgtcc atcggatgta aaacgcttac agaacgcgga cttagtcgtt
     3721 tgggttggcc cggagatgga agcgtttatg caaaaaccgg taagcaaatt actaggagcg
     3781 aagcaggtaa cgattgcgca gcttgaagat gtgaaactgc tactgatgaa aagtattcac
     3841 ggcgatgatg atgatcacga ccacgcggaa aaaagtgaag aagatcacca tcacggcgat
     3901 ttcaacatgc atctttggct ttccccagag atagcgcggg ctacagcggt tgcaatccat
     3961 ggaaaattag tggaacttat gccgcaaagt cgagccaaac ttgacgccaa cctgaaggat
     4021 tttgaggcac aattagcctc aaccgaaacg caggttggta acgagctcgc gccgctcaag
     4081 gggaaaggtt atttcgtttt tcacgatgct tacggctatt tcgaaaaaca gttcggactg
     4141 acaccgcttg gtcattttac cgttaaccct gagattcaac ctggcgcgca gcgtttacat
     4201 gaaataagaa cacagttggt tgagcaaaaa gcaacctgcg tttttgctga gccacagttc
     4261 aggccagcgg tcgttgagag cgtcgcacga gggacatccg ttcgtatggg aacgttggat
     4321 cccttgggga cgaatatcaa actgggtaaa acaagctatt cagaattcct gagtcaatta
     4381 gccaaccagt atgcgagctg cctgaaagga gattaatgag gaagtgatta cgtgcaacag
     4441 atagcccgct ctgtcgccct ggcgtttaat aatttaccgc gaccacaccg cgttatgttg
     4501 gggtcgctca ccgttcttac tctggccgtc gctgtctggc ggccctatgt ttatcaccgc
     4561 gatgccacgc caattgtcaa aaccattgag ctggaacaga acgaaattcg ttcgctctta
     4621 cctgaagcca gtgagccgat tgatcaagct gcacaagaag atgaagccat tccccaggac
     4681 gaactggatg acaaaatcgc cggtgaagcg ggcgtgcatg aatatgttgt ttccactggc
     4741 gatacgctaa gcagcattct caatcagtat ggtattgata tgggtgatat cacccaactg
     4801 gctgcggccg acaaagaatt gcgtaacctg aaaatcggtc aacaactctc ctggacatta
     4861 accgcggacg gcgaactgca gcgcctcacc tgggaagtgt ctcgtcgtga aacccgaacc
     4921 tatgaccgta ctgccgctaa cggttttaaa atgaccagcg aaatgcagca aggagagtgg
     4981 gttaacaatc tgctgaaagg taccgtcgga gggagctttg ttgccagcgc cagaaacgcc
     5041 ggtttaacca gcgccgaagt gagcgcagtg attaaagcca tgcagtggca aatggatttc
     5101 cgcaaactga aaaaaggcga tgaatttgcg gtgttaatgt ctcgagagat gcttgatggt
     5161 aaacgtgagc aaagccagct gctgggcgta cgtttgcgtt cagaaggtaa agattattac
     5221 gcaatccgcg ctgaggatgg caaattctac gaccgtaacg gtactggtct ggcgaaagga
     5281 ttcttgcgat tcccaacggc gaaacagttc cgtatctcgt ctaactttaa cccgcgtcgt
     5341 actaatccgg tgaccggtcg cgttgcacca cacagaggtg ttgatttcgc catgccgcag
     5401 ggtacgccag tgctttcagt gggtgacggt gaagtggtgg ttgccaaacg cagtggcgca
     5461 gcaggttatt atgtggctat tcgtcatggt cgcagctaca ccacgcgtta tatgcacttg
     5521 cgcaagattc tggtgaaacc gggacagaag gtgaaacgtg gcgaccgtat cgcgctttcc
     5581 ggtaataccg gacgttcaac cgggccgcat ctgcactatg aagtatggat aaaccagcag
     5641 gccgtaaacc cgctgacggc aaaactgccg cgtaccgaag ggctgaccgg ctccgatcgt
     5701 cgcgaattcc tggcgcaggc caaagagatt gtgccgcagc tacggtttga ttaattaaca
     5761 tccattcgca gccggtacgc agtcagtacc ggcttttttt atttggtgcg gggcaagttg
     5821 cgccgctaca ctatcaccag attgattttt gccttatccg aaactggaaa agcatggaaa
     5881 cgaaaaaaaa taatagcgaa tacattcctg agtttgataa atcctttcgc cacccgcgct
     5941 actggggagc atggctgggc gtagcagcga tggtgggtat cgctttaacg ccgccaaagt
     6001 tccgtgatcc cattctggca cggctgggac gttttgccgg acgactggga aaaagctcac
     6061 gccgtcgtgc gttaatcaat ctgtcgctct gctttccaga acgtagtgaa gctgaacgcg
     6121 aagcgattgt tgatgagatg tttgccaccg cgccgcaagc gatggcaatg atggctgagt
     6181 tggcaatacg cggaccggag aaaattcagc cgcgcgttga ctggcaaggg ctgcagatca
     6241 tcgaagagat gaggcgtaat aacgagaaag ttatctttct ggtgccgcac ggttgggccg
     6301 tcgatattcc tgccatgctg atggcctcgc aagggcagaa aatggcagcg atgttccata
     6361 atcagggcaa cccggttttt gattatgtct ggaacacggt gcgtcgtcgc tttggcggtc
     6421 gtctgcatgc gagaaatgac ggtattaaac cattcatcca gtcggtacgt caggggtact
     6481 ggggatatta tttacctgat caggatcatg gcccagagca cagcgaattt gtggatttct
     6541 ttgccaccta taaagcgacg ttgcccgcga ttggtcgttt gatgaaagtg tgccgtgcgc
     6601 gcgttgtacc gctgtttccg atttatgatg gcaagacgca tcgtctgacg attcaggtgc
     6661 gcccaccgat ggatgatctg ttagaggcgg atgatcatac gattgcgcgg cggatgaatg
     6721 aagaagtcga gatttttgtt ggtccgcgac cagaacaata cacctggata ctaaaattgc
     6781 tgaaaactcg cagaccgggc gaaatccaac cgtataagcg taaagatctt tatcctatca
     6841 aataaaaaaa agcctctcaa attattgaga ggctgtactc gataaaaaat ctcaatgagt
     6901 gttcccacta ttttgagttt ttaggttagt acgctattga gttaatcgta gcgtgataat
     6961 ttcaaaaggt ttgaactcaa gattgcaata ttgattgcaa taaaaactgt catctaactt
     7021 attttcttga agatcgacat attgaacgta gtgataatta catacccacg aaagcatggc
     7081 tgaaatttct aatccatgac tctcatataa tcttacgatt ataccgtcgc cgtcttcagc
     7141 taattttatt gtttcgatga taacgctgtt agtatcgata gcaaaaaatg atagtgaagg
     7201 gggtaattca cccgggtgcg gtttgacagg gacgacctgt aagggatgat tcattatata
     7261 ggcttgatta ataacaccac ccacacggta atctccatga tgaggaaata aggagaatat
     7321 gaatttttgt ttaccaatat ctgcaaaatt attaatcagg aaattatcat aattgtctgt
     7381 cttaacaact tccccaggtt tatgctgcga acgcagcaaa gttaatttaa gcgtctggtc
     7441 tttgacacga tagccatatt tgttttttgc tattaaagct atgccagttt ctcgatcaga
     7501 aaggtcgacc cagtgatgtg ctggtatttc atctttggca aaagcgaacg aatcatcgct
     7561 atgcgttggt ctgtcaatta caccgaactg aatttgacat tgtgcaagag atgtttgtac
     7621 tgacaccggg aatttgactt tgagagaaac attaggttcg tgccaatcaa taaaagtttg
     7681 gacatcaacg agtggactgt cctgtgtcag ggtaaatgcg aagcgaagat aagaggaatt
     7741 gtagtgataa atcagcgtca tcgtagtttt aggcccgctg attacagttg tcattttttc
     7801 agcgagtaaa acatatgatt ccccatcacg ataatttgaa gcaaaatccc aggccgcgaa
     7861 aaaacctgca tcttcgtggt atgcacggat ctcactgtgc atattttcag taatgtattc
     7921 tttaccatat cttttatcat atagcgaaat taaatcaccg ttttctgaaa aaagaagttt
     7981 aatgtaatta ttttcaatac ttcgagattc tgccttcaga ccatttacga tcttattctt
     8041 tggatcaatc acagcataac cataagagtt tactcgtgct ttgagccagt tgttgtttat
     8101 attgatccat tcgttacgag caaaacaggt ggtattaaat aaaatgtgcg gatctttata
     8161 agaagaagta tcaattttac ttaataaagt agactgcgta tcagaaataa ttttctcggt
     8221 ttttgcttct agtttaatat attccttttc agtttcaaga taaactcttg aaatacatga
     8281 tccgggaagg atgtcatgga attgcaaggt cagcatctct ttccatattt catcaaactc
     8341 actgcgaaga attgatgtca tattattagt aatagttgta aaaaattcag catcatgtaa
     8401 tttattttcc atatttcgat tatgagcttt agttgctgac tctgatgtaa agcacccttg
     8461 atgagcctcg aaataaagtt cacctgaaat aatgggcaag ctttcgcgaa aagcgtcggc
     8521 gtaagtgaag aacttatcaa cacggctgaa atcaacatga ggt
//
LOCUS       DAJVOY010000171         8550 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVOY010000171 DAJVOY010000000
VERSION     DAJVOY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.171"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            96..971
                     /locus_tag="OQL90_003414"
     CDS             96..971
                     /locus_tag="OQL90_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCS1370070.1"
                     /translation="MFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVTVEDAVRG
                     ACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVWTDFHALA
                     LRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGMDFRAQAD
                     AIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVLKAYDIIS
                     HRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATMHALFNEP
                     LQGESANTSNIVEIF"
     gene            971..1567
                     /locus_tag="OQL90_003415"
     CDS             971..1567
                     /locus_tag="OQL90_003415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370071.1"
                     /translation="MGKSKKNRAAATKQTQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILISTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRR"
     gene            1601..2002
                     /locus_tag="OQL90_003416"
     CDS             1601..2002
                     /locus_tag="OQL90_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1370072.1"
                     /translation="MYMTDAAQNQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGL
                     QIIPLSEVAAKDEFLNIKNVSRDDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLEFVR
                     NELIPLQKRMMEINKLVSENIVKFDSYKLAT"
     gene            complement(2109..2990)
                     /locus_tag="OQL90_003417"
     CDS             complement(2109..2990)
                     /locus_tag="OQL90_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370073.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(2974..4623)
                     /locus_tag="OQL90_003418"
     CDS             complement(2974..4623)
                     /locus_tag="OQL90_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1370074.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            complement(4916..5149)
                     /locus_tag="OQL90_003419"
     CDS             complement(4916..5149)
                     /locus_tag="OQL90_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCS1370075.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(5160..5348)
                     /locus_tag="OQL90_003420"
     CDS             complement(5160..5348)
                     /locus_tag="OQL90_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370076.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(5502..7916)
                     /locus_tag="OQL90_003421"
     CDS             complement(5502..7916)
                     /locus_tag="OQL90_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCS1370077.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(7913..>8550)
                     /locus_tag="OQL90_003422"
     CDS             complement(7913..>8550)
                     /locus_tag="OQL90_003422"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCS1370078.1"
                     /translation="RVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYR
                     GLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVV
                     YCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFT
                     HHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT         2309 a         2120 c         1766 g         2355 t
ORIGIN      
        1 ccttaaaatc accatcatac actaaatcag taagttggca gcattaccca ccatccagtc
       61 tgacccacag tgcttatccg ttctggtccg gtgcgctgtt caaccgtggg cgcaacaaag
      121 ctgacaaggt ggacatcgac ctgtcccaca gcaatctggc ccccggccgg ctgtgcgcag
      181 acgggcaata ccgccagata gtcaccgtgg aagatgcggt gcgcggcgcc tgtaacctgt
      241 tcgaccttga ccagttgcgc atggagtaca gccctgacga ataccagaac ctgctgatgt
      301 gcgagttcgt ggacgatctc gcgtccgtgt tcccgctcag cgaattgcag gcgtgcatgg
      361 tggacagctg ggaagtctgg accgactttc atgcactggc cctgcgcccg tttggctggc
      421 gcgaagtgtg gatcggttat gacccggcaa aaggtacgca gaacggcgac agcgccggat
      481 gcgtggtggt ggcaccgcca gccgtgccag gcggtaagtt ccgcattctt gagcgtcacc
      541 agtggcgcgg aatggacttt cgcgcccagg ctgacgccat caaaaaactg actgaacagt
      601 acaacgtgac ctatatcggc atcgactcaa ccggcgttgg tcacggggtt tacgagaacg
      661 tgaaagcgtt ttttcctgcc gtccgggagt ttgtctacaa ccccaacgtt aaaaacgccc
      721 tggtactcaa ggcctacgac attatcagcc accgccgtct tgagtttgac gccgggcaca
      781 ccgacattgc gcagtcattc atggcaatcc gtcgcgccac caccgccagc ggcaaccgcc
      841 cgacctatga agccagccgc agcgaagaag ccagccatgc cgatctggcc tgggcaacaa
      901 tgcacgcact gtttaacgaa ccgctgcagg gcgagtccgc caataccagc aatattgtgg
      961 agattttttg atgggaaaga gtaagaagaa ccgcgctgcg gcgacgaaac agacccagca
     1021 taaaaaccag acttcagccg aagcattcag cttcggcgat cccgttccgg ttctggaccg
     1081 ccgtgaacta ctggactatg tggaatgcgt acagacagat cgctggtatg agccgccagt
     1141 gagttttgac ggactggcgc gcaccttccg cgccgccgtg catcacagtt caccaattgc
     1201 ggtgaaatgc aacattctga tcagtaccta catccctcac ccgctgctca gccagcaggc
     1261 tttttcacgt tttgtgcagg attatctggt atttggtaac gcttacctgg agaaacgcac
     1321 aaaccggttc ggagaggtca tcgctcttga gcctgctctg gcaaaataca cccgacgcgg
     1381 attagaccta gatacctact ggtttgtgca atacggcatg accacgcagc cgtatcagtt
     1441 cacgaaagga agcatctttc atctgatgga accggacatc aaccaagaga tctacggcct
     1501 gccaggttac ctttctgcca ttccgtcagc cctgctcaac gagtccgcca cgctgttccg
     1561 ccgatagtat tacattaacg gcagtcatgc aggcttcatc atgtacatga ccgatgccgc
     1621 gcaaaaccag gaggatgtga acaacctccg caatgcgatg aaaagcgcca aaggaccagg
     1681 taacttccgc aatctgttta tgtactcgcc taacggcaaa aaggacgggc ttcagatcat
     1741 cccgttgtca gaagtcgcgg cgaaggatga gtttctgaac atcaagaacg tgagccggga
     1801 tgacatgatg gcggcgcatc gtgtgccacc gcaaatgatg gggattatgc ctaataatgt
     1861 cggggggttt ggggatgtgg agaaggcgag cctggagttt gtacgtaacg agttaatacc
     1921 gttacaaaaa agaatgatgg aaataaataa attggtaagt gagaacattg ttaaatttga
     1981 tagttataaa ctagctactt aacgtaatca ctaaatgagg ttctttaaca cagctaaaaa
     2041 atttattaat agccagcagt ttttatgaat ttcttcagcg tgcagataca cgctgaagat
     2101 gcaaaaaatc atatgagacg accgtctgga cttctgttac ctaaatgtaa ttcacagagt
     2161 atccacctaa tcacatcagg tcgaattaac ccagctacaa aaaccgaatt ccaattattt
     2221 ttacctttat gtttttcatc caacagcaac aactcgcgag tcaacaactc atatacatcg
     2281 ctataagtag aaagactctt attcagtcct ctaataacaa gctcaggata taagtaaaac
     2341 gactcccatt gttcagacaa atacccctta atattattct tgataacaac attatcaaga
     2401 tgatacttaa cattgttata ttctggtgac attgggtcaa ttatgatttt tacatcaatt
     2461 gtaggtgttc gccctaattt agaaaaatta gcacatatta atctttgttt taatatatta
     2521 tcaaaatatg gatgcagcat ccgatggcct gcagaattag ctatagtagt acttttgaaa
     2581 gtattacatt tgcaagcagg aaccaaattc ttagtgaata aagaaaattc tggatagttt
     2641 tccttgggta aaacatgatc caaagtcccg ctatgcattg aaccacacat tggacataga
     2701 gagttagaat tagctgctct tatatcatca attatagata aacactttgc gggattgtta
     2761 taataatatc tcaaagcttc agcaatattg ttactcaata cgccacaaga attgacatta
     2821 agataccctt tattctttat gtacgatcta tatgcattaa gtataaatgt atcctgtcca
     2881 ttcagaacag aatgatgttt taatctctta tttgcagata gttttttaaa tgcagaataa
     2941 tctttaaaaa caggtctttt taatttattc atttcaattg cccatttcat ttctcaacgc
     3001 agttaataca ttaagtgata agtcctcgct atatttagat tcaacatcat cccaagacat
     3061 attctttgca attatattgt tttttacttt atttaaaatt tgagttggtt cactctcacc
     3121 gaaaacaaaa tatgaaatat ttcctatatt cgccccaaaa gtactcaatc tcggtttttc
     3181 gctaacaatt accccatcat ctcgtcttct gagtattgtc acttgctcct taaaaacctc
     3241 ccgaacaaga taaaccgaat gcgttgctat gatagctgaa gagcctgtct tttctaataa
     3301 attatcaagc aatgccataa atttattaat aaatgaagga tggagatgtg tttctggctc
     3361 atctattaat agaagagtcc cattctcaat atacaaacag acttgtacac aaaacttaat
     3421 aaacgatatt tccccactac tcaaagggta acatttattt tcaataagcc tcaaaggctc
     3481 gccagagaga ataatttcag aatataatga tagtctagat tcttcgcctc gagaattaag
     3541 ctcatttaat ttaacaaatg gtgaattttc accgccacat ttgattacta aatcgttgtt
     3601 actatttaga acactcagtg catcaacaaa tatattccac ctttgattat agccgatagc
     3661 gtcgttactc ctcgctagtt gcactattaa atcattcaag tttaatttgg attttgattc
     3721 acgatttaaa gtcaatcttt tgtaccatat caatgatttt attcttttat cggttggaaa
     3781 tgaacactta ctttcatttg ttggagaaaa agcaatgagc ctactaattt gaattcgttc
     3841 ttcaatgctc acaccgttag aatcaactac agccaccaaa ttatcctttc ctttaatcgc
     3901 agccaatgcg atttctctaa gcgtttggct ttttcctaca ccattctcgc ctataattat
     3961 ggaaattctt tttggtaaat catgctcgtg atcaaaatta aaaacataag atacatcttc
     4021 ttcattaact ttattcgaat agctaatctt gatgtgctgt gataaattac ccaactcctc
     4081 atattgaata ccggaaagaa ctgttcctgc atttttgaaa gtaaaatacg actcagactc
     4141 tctaatgaaa gatttattaa agatatcaga agagattgct tttttcctca aattctctgc
     4201 actagcgtca cttttcttag aaacaacatc attaatgaaa tttaatatat atttggattc
     4261 attcacccca aaaaatctta ctagtcttcg ataattattc atatcctgaa gcatgacaaa
     4321 ataatcatta aacccctcat cctttaaatt agattcttcc tttttcttca ctcgccttat
     4381 cagttctgct tttccatttc gttcctcctc tgaattcaaa aaaccaatat aaccattcag
     4441 tgaaattata tccttacact cttttttatt aaaaatagat atttcgactc tactcaaata
     4501 tccaaaatca ttccaatcgc ttcttgatgg gactattttt atataattac cagaatcaga
     4561 tatctgttga ttatttgtaa ttaaaaaata taaactataa tttttatttt tcttgactaa
     4621 cattattacc ctctcacttc attagtgatg atttattttg gaattatttt acacacttat
     4681 acctagcgcg caatcgtatc cccgccacgc ctgcccgctt tatgtagagg ttttcatgca
     4741 cctgcatgat ctacgcaaaa gcccgccagt tctggcgggc cttagcaaaa acgatcctca
     4801 aacgatcatg cgatctcatg cggcatagac atgcactaca gagctaacgc ctcgcaaggg
     4861 ctcgttgttc aaccttgctg acgccagaag ctagttcaga cgccagcaac gtttcttaat
     4921 gcagccagct gtcgtcttcc cacaccttct gcataatttt catcacttgt tttctttctt
     4981 cgtccagttg caatccagta agttccacac cgttagagct acctttgcgg atacgaatta
     5041 ccgttttggg atacagaggg cgcagattgc ggtaaagctc ggattcaaga gcgtccaggg
     5101 tagactggct aatcttctgc tctttatcga tcattatttc aatgcgcata aaagtcacct
     5161 cagctgatga catccattga gcggttgtat tcgtgggttc tgatttttgc catgagttca
     5221 tcagtcaatt cagaaaccca ctgcagagcc agccccttct cttcatcact acactcacta
     5281 gccgctacaa gcttaagaaa aaaatcaatg cgctggagct tcaaagactc caaaaaatag
     5341 tcctgcatct ttcctcctat gacaccacac gcaatactgt atgcataacc actgtttata
     5401 tttacagcat ataataatct tactgatgta aaacgttttt ttacgttcat cagcctgata
     5461 tgcctggtat tattaagagc acgaattgtt aacccgcgta attaatacag gttccgccac
     5521 tgatcatctt cctgcaaacg ctggttccga tagaagatac gcaggcctgc tcctgacgga
     5581 atactgcctc cgcgaaggag taaatcgacc tctttctcgc tgccatcaaa tcctctggac
     5641 ttcagctcat acacgagctg cagtcgctga tggtctgtaa ttcgctgttt gtagtcttta
     5701 cgccgtttcg gtttcaccag gcgtaacctt gctgccagtt cccggcgctc ttttttgctc
     5761 atactgtgca ggtaatcgtg caactccttg tcatccatgc tggtaatgtc cgttctgggg
     5821 ttcccatcag ctgatttatc tttctcctgt tggttcaaat tttcagcaag gagacagtta
     5881 ttgccacgag tccaaggggc gcaagcgccc tggtcggctg ccgcctcctg aacgtcaacg
     5941 gctttacgaa ccattttcca cttcactgca tgagtgcaga tcttgccttc tgcaatgggt
     6001 gaccagatgc cataaatacg aataccgtga tcgccatagg cggtcggctc ttcattgatt
     6061 tcataagcgg ttctgatcaa gtgatatttg cgaggaacca gcacgccgcc ctgcctcata
     6121 atgtaggtgg caaaacagcc agcatcagca gcagccagga tggcatcaag gcgcgggtta
     6181 tccagtaccg gcgcacctgc ttttttgtca ccctgctgcc ttgccgcctg accagccaac
     6241 aaccgcagtt cacggtaagc ctgacgccct ggaataccaa agaagcggaa ttgctgaaca
     6301 cgatgcagag acgcccaggg attaacgtat tcagcgttat cacgcaggga tttacccgtt
     6361 tccttgctga tctcgccagc cagaccacgc ccgtcaatgt tcttactgat gtatttcgcg
     6421 atgtagcttg tcggcgttcc tttgcgcggg tttatcagct cagatttaaa gcgtggacca
     6481 gtgttattac ccagttcctc gcggtcttca cggatggcaa acttacgcaa caatgcagta
     6541 atggcgcggc ggtctttttt gcgcataaaa cacaacaggt gccagtgaac tgtaccgtca
     6601 tgatgcggct cagccacccg cacgccatac cagcgcaacc cggctttgtg catcgcctta
     6661 cgaaatgcag caaacatgcc gaccagataa tcgctgcttt gtcttaccgt cgcatttgtc
     6721 caggttgggt ttggtctgcc gttattgagc gtggaatgga aacgtgacgg acaggtaatg
     6781 gtgtagaaaa cggcgcagtc accgcgcatt tccgcgataa gctccagacc tttaacacag
     6841 gccatcatct cattgcggcg gtgcgccggg ttgctgctgc tggcgtttac cacgtcttcc
     6901 atatccagcg tgtcgccctc ttcgttcacc agttcatgag aacggaaaaa ctccaatgac
     6961 ttgcggcgct gctcacgttt atgcatcacg gcttcatagc tgacataggg agatgctttt
     7021 ttgctgacca ggcagacagc acgcaactgc tcttcccgcc attcgcaacg cattttccat
     7081 aatttccggt accaccagtc ggcgcacaac atacgcgcca gcgaacccgg aatgagttca
     7141 tagggcacgg gtttacggcg gtttcttttc cgacggagtt gctcaaacgc aggcgggatg
     7201 acatccagac gcagggtttc cgctgccacc ttttcccatg tcttgcggat ttcttctggc
     7261 ttaacgtcat cggtggcata caaatcacca caagctgcat caaggcacat gctcatatgc
     7321 gcagcgacaa gggtagacag gcgtttcacc tgatcctgac tcatttcagg cagaatcagc
     7381 aggccgtcca gccctacatg gcttgccata aaacgaaaag atgcagatag ctggctgtcg
     7441 cgtacatgct ccagtcgttc cagacatggc ttaatcgtct cacgtaaata gcgggaataa
     7501 gcctttggcc tgcccaggct gctgaagtat tcaatacgtt gcatcagcgg cttgctgata
     7561 tgggaaggct gggcgttgac gtccgccaga atgaccatat ccggattaaa acgctgctgc
     7621 tcatgcgcca gctttgcccg gctaatgagc ttatcctgct ccatttcgtg ctggacagga
     7681 tcacgtgatt cataaaaaaa ataacgctcc cagacctgat cactcagtgc ctcgcggcgc
     7741 aactgttcct gctcgttatc ggcagcgtac agagtgatca ggtttgaaag cgcagaaacc
     7801 ggcgcaactt ccgccgggtc cagataaggg ttaatggcct ttttcgggct gttccatgag
     7861 aacgctgcgg cagcctcgtt aaagccgcag cagttgttca tatcggcatg gctcatgcac
     7921 gtactccgta cacggcagaa ctatccacgc cacgcgaata atcaaatccc acccagcagc
     7981 gcggcccgga aacagcaatg atttctgttg ctgatttacc ctcgccagct gccacaccga
     8041 tgctgcgttt taccttgata tagtggtgag taaaattgcg atacagcgaa cggatcaggg
     8101 atgtgtcact gttagaaaca atgaccggat gtccttctga tgaccgatgt tcaagaacgg
     8161 atgctaggtg atactggtca tcttcagtga aaccatcagt gtgatagccg gaaaacgtac
     8221 cgtcatacgg tggatcgcaa tacaccacat cccccgcctt caacatcgcc agcgtttcat
     8281 caaagctggc gcagataaac gttgctcgct gggctttttc tgcaaatgcg cgaatttctt
     8341 tttcagggaa atacggattt ttataattgc cgtagggaat gttgaaatgc ccgctcttgt
     8401 tatagcgaca taaaccacgg taaccgtgac gattgagata caggaaatat accgctttca
     8461 tgaaatcagt aatttcagtt gagtaattaa actcctgcct tatgttgtaa taagccacct
     8521 ccctgtttgc gatctcaaat aaaactctgg
//
LOCUS       DAJVOY010000172         8264 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVOY010000172 DAJVOY010000000
VERSION     DAJVOY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.172"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..77)
                     /locus_tag="OQL90_003423"
     CDS             complement(<1..77)
                     /locus_tag="OQL90_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370079.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVI"
     gene            complement(88..786)
                     /locus_tag="OQL90_003424"
     CDS             complement(88..786)
                     /locus_tag="OQL90_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1370080.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(786..1127)
                     /locus_tag="OQL90_003425"
     CDS             complement(786..1127)
                     /locus_tag="OQL90_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370081.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(1120..4380)
                     /locus_tag="OQL90_003426"
     CDS             complement(1120..4380)
                     /locus_tag="OQL90_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1370082.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATEHLISLADEWGSVNA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQAWESA
                     RQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQ
                     YVLANPGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLA
                     GLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRL
                     PQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYIS
                     NALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTA
                     QGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQ
                     GERLRERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQE
                     AFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGSWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            4425..4754
                     /locus_tag="OQL90_003427"
     CDS             4425..4754
                     /locus_tag="OQL90_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCS1370083.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            4741..5133
                     /gene="tnpB"
                     /locus_tag="OQL90_003428"
     CDS             4741..5133
                     /gene="tnpB"
                     /locus_tag="OQL90_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1370084.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            5140..6741
                     /locus_tag="OQL90_003429"
     CDS             5140..6741
                     /locus_tag="OQL90_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCS1370085.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(6876..7136)
                     /locus_tag="OQL90_003430"
     CDS             complement(6876..7136)
                     /locus_tag="OQL90_003430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370086.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(7184..7393)
                     /locus_tag="OQL90_003431"
     CDS             complement(7184..7393)
                     /locus_tag="OQL90_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCS1370087.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(7489..7863)
                     /locus_tag="OQL90_003432"
     CDS             complement(7489..7863)
                     /locus_tag="OQL90_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCS1370088.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(7878..>8264)
                     /locus_tag="OQL90_003433"
     CDS             complement(7878..>8264)
                     /locus_tag="OQL90_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HCS1370089.1"
                     /translation="FLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLD
                     KTLTVNTGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTC
                     VVTDSAEKAQSVTSVECTVTVSAAAG"
BASE COUNT         1786 a         2611 c         2016 g         1851 t
ORIGIN      
        1 cttatcacga agccgcacga ctccgcaggc gcacaccgcc gggcatgcgc cagaatcgct
       61 gattcagtct gtgtcataaa tcgggattta ctgcgaaagt ttattaatgg aaaggaaacc
      121 gccaaaattg ccgacattcc tgcgcagttc acacccgcgc atgcacttgc tgcatctgtc
      181 cttacggata tccgtggtgg gtttatcgaa ctcatccgcc acagccccgc ccgtgtaacc
      241 acactcatca gagcggtagg tccacataca ggtgttcgcc agcatgatac gaccgggaaa
      301 cagcgccccg tccgtctcgg tcggtgtagc cagcacaaac gaggccgtca tggctgtcag
      361 ctgcgacatc tgctccacca cccagcggtc actcagctcc tgctccgggt ccgcctccgg
      421 attgcccgca acgaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac
      481 cgtggccccc accagactct gcaggtcttc cgccatcccg gtgaccagac cgaacagatt
      541 ggacaccgtc agcgacggtc tggcactgct gccccggccg ttcatctcaa agccgctgcc
      601 gtcaatcggg tatgcctcat acttacgccc ctgccaggtg accggctccc ctttttcatt
      661 cagctcatta cagaaaaaat accgctcacc accctgcacc gtcaggtcaa tttcccagag
      721 taccacccgc ggtgactgct ctgatttaac cgactcgctc agactttctt cgtgaatatc
      781 ctgcatcagt tcaccacctg ctcaatcgta caactgaaat cactgtacct ggcgttatct
      841 gtgacgctcc actcccggca taccaccctc accgtccggt tatgtttcgg cggtcgccac
      901 aaaaaggcac ggtaaccacc atgccacgat aaaaactctt ccagccatcg ccgggtcgcc
      961 tcatccgtca cccggaacac cgcctgaaac gtcttcagtc gggcattaag tcccgtcggg
     1021 cggcgctgtt cataaccgtc accaaaccgt accctcgcca ccgacggttt ctcactcacc
     1081 tgcatccctt cacgcgggac cagatgcagc gtttttatct cagccactca gcattcctcc
     1141 gtcacgtcgc atggacagca tcaccgcctg cacccgctgg tcaatcagtt gcacaaggct
     1201 gcccgcagct tccggcccta tctggccatt agtcccgtca ttctgaatgg cgatatggta
     1261 gaccggggaa tacaccagac ctgcactgcc gttcatactg cccaccgctc gcacacccag
     1321 cgagccatcc gccgcccggg tcaggggcat aatggcttca ggaccggcct cccccatcag
     1381 tcccgcccct tttgcaaagg caaagtacgt gggcgtatcc acaatactgt tgctgtacgc
     1441 actcaggttt gccgaggtat acacaccgcc ttttgcattg gccaccgcac cgcccagcca
     1501 gctaccaata ctgccgataa atcctcccgc tccggacata ctgtttgccg ccgtcttgat
     1561 cccgttgaca atcgcggcat tcataagaac ttttgatatt tcctgcagca ctgatgaggc
     1621 ccagctgcgc cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc
     1681 tgagataccc tccgtcgcaa gctgtgctgc ctgtgaggcg taatcggacg cattatccac
     1741 ccagttactg aatccctcct gcagcccttt ctgccagtcc gcacgctgca catccgattc
     1801 ggcataaaag gcttcctgct ctttcagacg ttcactcaga tactgcgcgt tctgtgccag
     1861 agcctgtctg taaaaatcct cactgatatc cccggtctga tactgagact gaaggtccgc
     1921 atccttctgg cggaaactgt cgcggatctg ctgcaactcc cgcatgcgtt cacggagccg
     1981 ctctccctgc ccgtacccca gcagttccgc ttcatttgat gcacgagcag ccacattatc
     2041 attcttcagg gtctcttccc gggaccgcaa ctgctcccgg attttttgct ggtcaatcag
     2101 ggccgcattg cgcagcagtt cctgcttctg catctccgtc agggttttca gttcgccctg
     2161 cgcagtctgg tatttcagct tcgccagctc tgtattctgc cccgccagtg ccagttgctc
     2221 tttctgctgc ttcagcagcc gggaaaaact gtcttccgct ttttccgtct ctgattttcc
     2281 accccgggat ttgggtttat tcgcctcgtt attgcgccag gcttccaggg cattactgat
     2341 ataacgttgt ctcgcctcct gatacggatc acccacaaaa ccgaggtcat ccgccgcata
     2401 ccccagtcgg acacgctctt tttcttcccc tttcagtctg gacagggcca gctcacgctc
     2461 tgtttttgtc agggcactct gctgtttatc atccagagtg gcctgtggca gccgtaacgg
     2521 cacattcacc agtccctgcc gctgctgaag cagttcatta cccagcccca gcagacggtt
     2581 gaattccgta tgctgaccgt tcataaccag catggactgg tacaccttat tctgctctgc
     2641 cgcctgctga cgaattaacg ccacacgacg gtcttccagc ccggcaagca catcctgaat
     2701 ggactgcgct ttttcctgca tcagtgccag acgggactgc tcaacggcaa gctgctctgt
     2761 tgcctgagca agcccttccg ttacggtctt caccgaggtc agatggttta tcatgaatcc
     2821 gtcaccggtc gtccagcccg ggttcgccag aacatactga tatcctgcga ttttttcctg
     2881 cagggatttc acccgactag cctgttcatc aatcagccgg ttctgctctg tcagcgccgc
     2941 ccgtgttcgt ccttcattat ctgaggcttc aggcaaagac attgacggcg ttttatgcgc
     3001 gatttcatct atcgtcagtg catactggcg cgcagactcc caggcctgct cctgattctg
     3061 gtacagcgtg taccatgctg cagcccccag catcaccagt ccgggtacgc caccaaccag
     3121 ccccagcgca ccgctcatca gacgtgagcc caccgccgtt gtactgttca gcgcattctg
     3181 ggcggcgctt ctggcagcaa tatttctgtt caggcgttcc tgtgtggccg ccagacgggc
     3241 ctctgcagca atctgcatct ccgtcccgcg ggctgccgcc acggcctgct gagcacggta
     3301 cacggctgcc cttgcccgcg ccgtggcaat ctgcgttccc ctgaactgtg cttccgccag
     3361 tgcaacttca ttacgtgcag ccgtcacaag tcctgccgtg gcagacatcg ctccggaggc
     3421 catattgcca aagtaccggg caaccccgac ggcaaccagc gcccccacag ctgttgccac
     3481 attatcaatc tgtccggcaa caccgttcag cacgccggag agcgtttttg tcaccccgca
     3541 ggcctcattc acttcgccca cccaggccat aaaggcgttt tccaccttcg tgatactacc
     3601 ggaaaccgtt tccggcatgg ccgcatattc atcacgtaat atccccagct ggctgattaa
     3661 cgcagggacc actttatccg ctgtcagttt tccgtcatcc gccattgcct tcagatcttt
     3721 acgggccaca cccatccccg cagcaagcgc gcggatcacc cggtcgccat tttcgttaac
     3781 agcattaaat tcttcgccac gcagtacacc ctgtgccagt gcctggctga actgggtgat
     3841 caccgaaccg gattccgcaa ctccagcccc tgacagtttc agtcctgtcg aaatggcctc
     3901 cgtcaccttc agcacatcat cagcactgta accatattca cgcatcgagg cagccgaacg
     3961 ggcaaacagg gccgcattat ccgaaaacgc ggtacctgtt cgctgactga tatccatcag
     4021 cactttctgt gatgacgaga attcatccga tgactgagat gcctgtttca gacgggcatt
     4081 cacggaaccc cactcatcgg ccagcgaaat caggtgttcg gtggcaaagg caccggcaaa
     4141 tgcgccagcc attccgacag cagaaccgcg aatttccgtc aactgactgt tcagttctgc
     4201 aagggcgcgg cgctgttccc gtgcggcagc agcggcatga cgtccgccat tctgcagggt
     4261 ccggtaatat tcactgccca tacgggacgc ccgctggatc tccgactgga atgactgtga
     4321 atttgccgaa attttgagtg tcgttacggg gaagtcatct tcacagggat cttcggtcag
     4381 ttagtattac cgacatccat tcaccaactt accggagata atcgatgtca aaaccccgat
     4441 ggaccattga acagaaaagg cagcacgttg ctgcctggcg cgccagtggt cttacacgtc
     4501 agcaatactg tgaactcaac gatattccct tcacatcact tcgcgaatgg ccgcaggatg
     4561 tcgcaaaagc tgaacgccgg gcaaatgaac cgaccgttct tcccgttcat attgcgccac
     4621 cactgcatgc tgatgtacca cagcccgtca cgaatgagcc agtcacgctc ttccttcccg
     4681 gtggagtacg gatgtgctgt cagccgtcac aacttaccga tgtcttcagg gcactcaaat
     4741 atgctcagtc ctgataatgt cttcatcgca attaaacctg ttgacatgcg ccggggcatc
     4801 gactcactga cgcagtatat acaggatgaa ctccggtcga catggcatga gggagccgcc
     4861 ttcgtctttg ttaacaaagt ccgttcgcgt atcaaagttc tccggtggga taaacacggg
     4921 gtgtggttgt gtacccgccg tctgcacaaa ggcagcttcc gctggccacg tgcaaatgac
     4981 gctgcctggc acctcactcc cgacgagttt aactggctga ttgccggtgt tgactggcag
     5041 caggttaagg gacatgacct gacgaaatgg gtctggcaga atgaacctga actgcgccct
     5101 gaaaacacgc aaaatacact gctaactcag tgaaaaataa tgaatatccg tatctggagt
     5161 ggtatactcc cctgtatgga tatctccgct ctcaacacca cgaatgacat cgaaaaactg
     5221 cgtgctatgg cacttgccat ggtacaagaa gtcatgtcgg agaatgccga aaaagagcgg
     5281 gaattactgg agaaaagccg gcgcatccag cttctggaag aaatgctgaa actggttcgt
     5341 caacagcgct tcggaaaaaa atgtgaaacg ctggctggta tgcaacgctc cctattcgaa
     5401 gaggatgttg atgccgatat cgccgcgctt accgcacatc tggataaact gctcccgcaa
     5461 tcccctgaag aagatgaaaa agcgtcccgt tcacgcccga tacgcaaacc cttaccggtt
     5521 catcttccac gggtggaaaa aatgatccag ccggacactg accattgccc tgaatgtgac
     5581 gagccgctgc actatatccg cgatgcggtg agtgaaaagc tggagtatat tcccgctcac
     5641 tttgtggtga accgttatgt ccgtccgcaa tacagttgtc cctgttgcca gaaggtgttc
     5701 agcggtgaaa tgccggcaca tatcctcccg aaaagtgccg ttgagccatc agtcatcgca
     5761 caggtgatca tcaataaata cggtgaccac ctgcctctgt atcgccagca acaggtcttt
     5821 gcccgttcag atgtcgggct gcccgtcagt tcgatggctg acatggttgg cgcggcgggt
     5881 gccgcattat ctcccctggc ggcgttactc catcgcgagt tgataaaccg tccggtggtg
     5941 catgcagatg agactaccct gaagatcctg aacacgaaga aaggcggtaa atcctgctcc
     6001 ggttatctgt gggcatacgt cagtggagaa aggacgggac cgtcagttgt gtgcttcgac
     6061 tgccggaccg gacgtagcca tgagtatcct gaaaactggc ttcagggctg gggcgggacg
     6121 ctggttgtcg acggacataa agcttaccgg actctggcaa acaaagtgcc ggagatcacg
     6181 ctggccggat gctgggccca tgcccgcagg ggcttcgccg acctgtataa aatcagtaaa
     6241 gatccacggg ctgccatagc cgtgaagaaa atcgcggggt tgtaccgtct tgagaagaag
     6301 atcagtagcc gccccgtgga aaaaatccgc cagtggcgac agcgttatgc ccgtccgata
     6361 ctggaagaac tgtggtcatg gcttgaagag caggaaccgc aatgttctcc gggaaaggca
     6421 ttacacaaag ctattgccta tgcgctgtct catcgcgtgg aactgagccg cttcctggaa
     6481 gatggtgcgg tgccgctgga taataatgtg tgtgaacggg ccatcaaaaa cgtggttctg
     6541 ggcagaaaat cgtggctgtt cgccggttcg cagatggcgg gagaacgcgc cgcgcaaata
     6601 atgagcttgc tggaaaccgc gaaacgcaac ggtctggagc cgcatgcctg gttgacagac
     6661 gtcctgatgc gtctgccgga gtggccggag gagcgactgg cagagttgct gcctcttgag
     6721 ggatttacct tctccgggtg agtgatacct gccgtcaggt gttcgtgcac cgggccataa
     6781 cctgcagtcg ggagttgaac tcctgactgc aggaaatgag ccagaccagc aaccacgcgg
     6841 gctgacagcg aataagaacc tgaatcagcc cggagtcatt cccgggtcag tacatcatca
     6901 cctgcaatta atccccgagt cagtacttcg cggcctgacg cgatcacgct actcagtact
     6961 tcagagcccg cctccggtac ctgcctggcg atcgcctctg tgcaacaaaa taaatcaacc
     7021 gttatcaaac cacgagcgtt cgtatgcgcc gccgatgacg cgcgcctgaa aaacgcgtca
     7081 atatgagttc cccggaagct tacaattttg ataatcagtt cacgtaacgt cgccattcac
     7141 ctttctccgg gcgtaaaaaa accgcctcag cggttctcat cattcatgac tgtgctgcaa
     7201 agctcagcgc gtcttccagc gccgcaaacg gatccacctc cggcttatcc tcatcctcgc
     7261 cccagcagag catgacgtcc ttcagtgcaa cattcatccc ctgtgccccg aaaaccgctt
     7321 tcacgatctg tgcattacgg atatccccgc gctcatcacc cagcggggat atcctgtcga
     7381 actccatcca catcatcgcc tcgctcgcac tcaggctgtg gcgcagttcg gataaggtgc
     7441 gccccagacg gagcgcaagt cgcatcagaa agcgaatttc cgggcgggct acttttttct
     7501 ggccgactct gcatcagcga tcagttccag tgcctgacgc agcaaccggg catgtaccgg
     7561 accatagacg gccagcacct gctcacggtc gtccggagtg aacacccgct gcaggtccgt
     7621 atcacacagg acatcgcaga acagcgtcac atccgcttcc aagttacggc gggttttcgc
     7681 caccaccgac agggtatcgt catcctctcc atcaccattg agcacttcct gccacagata
     7741 ccaggcctct gccgaaggct cccgcagcac cacgctgaca ttcccccatt ccggcacctt
     7801 caccgtttta tgacgaaacc ctgacagtct ggccagcgcc agcgttttca gatccttttt
     7861 catgatgacc catcccctta tccggcagct gcgctcactg tcacggtgca ttcaacagac
     7921 gtcacactct gtgctttctc tgccgaatcg gtcaccacgc aggtatattt ccccgcatca
     7981 gcggactgcg cacctggctt actgaaggtg tctgtcgtct gcccgtcaac cggctgacca
     8041 tccttcttcc aggcgtattt atacggcggc attcccccgt tggcactgac tgacattgtc
     8101 agcagcgcac cggtattcac ggtaagtgtc ttatccagat ttttcacaaa cgccagcggt
     8161 accacaaagg acaccggttt gcctttcaga cgcagtgaga acgttgcagc caccacaccg
     8221 ttggtaccgg atgaccaggt gtgctgacgc acttccgcca ggaa
//
LOCUS       DAJVOY010000173         8211 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVOY010000173 DAJVOY010000000
VERSION     DAJVOY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.173"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(60..2603)
                     /gene="mdoH"
                     /locus_tag="OQL90_003434"
     CDS             complement(60..2603)
                     /gene="mdoH"
                     /locus_tag="OQL90_003434"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1370090.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2596..4131)
                     /gene="mdoG"
                     /locus_tag="OQL90_003435"
     CDS             complement(2596..4131)
                     /gene="mdoG"
                     /locus_tag="OQL90_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1370091.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4525..5682
                     /gene="mdoC"
                     /locus_tag="OQL90_003436"
     CDS             4525..5682
                     /gene="mdoC"
                     /locus_tag="OQL90_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1370092.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5690..7171)
                     /gene="clsC"
                     /locus_tag="OQL90_003437"
     CDS             complement(5690..7171)
                     /gene="clsC"
                     /locus_tag="OQL90_003437"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS1370093.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLVAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7113..7646)
                     /gene="ymdB"
                     /locus_tag="OQL90_003438"
     CDS             complement(7113..7646)
                     /gene="ymdB"
                     /locus_tag="OQL90_003438"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1370094.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7742..8056)
                     /locus_tag="OQL90_003439"
     CDS             complement(7742..8056)
                     /locus_tag="OQL90_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1370095.1"
                     /translation="MNVQTFFKLSYARQFFFPFIAIAGSTAQGGVIHFYGQIVEPACD
                     VSTQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIE
                     YN"
BASE COUNT         2044 a         2151 c         1952 g         2064 t
ORIGIN      
        1 caataaattg caggaacgat gtaggcctga taagcgtagc gcatcaggca actacgtttt
       61 tattgcgaag ccgcatccgg tttacgcaat gccagtggat tgagctttat cccttcgtaa
      121 taactcaccc atgaagaata tctctccggg gaattccaga cacggaaatg cagacgggcc
      181 atcgtcaccg gatcgcttag cagcaccagg cgacgatcgc gattcagctt ctctggcgtc
      241 tcgttaagcg cctgttcaac gtggcggtca cgggcgattt ccagcacctt actggcgcgg
      301 tgacgcgcgg tcgccattgc ggttgccaga gcgttaaatg acgggttaaa caccgcgtgc
      361 ataaaaccat catcaaggga gcgttgacga ttcatctcaa ggaagcgatc ggtatcaacc
      421 agcacctgcg gcggtgaata ctcttccggg atcaggaaca gtttccagtg tttggttcgc
      481 agaccaacgg tggcacggct ggaaatcacc gaaacaaacg gtgacaggat caacgagaag
      541 acaatcggtg ccagccagaa caggaaacgc agatccagcc acgccatccc aacagcccac
      601 actaacccta acagcagctg tgagccgtgg cgtttgaacg cttcacccca ggaagtggag
      661 tcatcatcac gctgcggtga attccacacc acttcccagc caaggaacgc gctgacaacg
      721 aagaccgtat ggaacagcat gcgtaccgga gccagcagca cggaaaaaag cacttccagt
      781 agcaacgata atgtaacgcg ccagaagccg ccgtattctt tcgttccttt gcaccagata
      841 agcaaaatgc tcaataactt cggcaggaac aacagcacca tagtcgaagc aaaaagtgca
      901 atcgccagct caggacgcca ctgcggccac accgggaaca actgccgtgg ttgcaggaag
      961 tattgcggtt cggtcaacgc atgtactacc tgcaatgcag tagagagcgc gaggaacata
     1021 aaccacagcg gagcggagag ataagacatc acgcccgtca ggaacaccgc acggtgaacc
     1081 gggtgcatac ccttcaccag gaacagacgg aagttcatca ggttaccgtg gcaccagcgg
     1141 cggtcacgtt ttagctcatc aagcaagtta ggcggtaatt cttcataaga acccgggaga
     1201 tcgtaagcaa tccagacccc ccaacctgca cggcgcatca acgccgcttc tacaaagtca
     1261 tgtgacagga ttgaaccggc aaaggaacct tcgcccggca gcggagccag tgcgcagtgc
     1321 tcgataaacg gtttcacgcg gataatcgcg ttatgtcccc agtagtgcga ctcgccaagt
     1381 tgccagaagt gcaaaccggc tgtaaacagt ggcccataca cgcgggtcgc gaactgctga
     1441 cagcgcgcat acagcgtatc cataccggac gctttcggcg acgactgaat gatcccggcg
     1501 ttcgggttgg cttccatcag gcgcaccagc ccgcacaaac aatcaccggt cattaccgag
     1561 tcagcatcca gcaccaccat gtagctgtac tggctgcccc agcgacggca gaagtcatcg
     1621 atattaccgc ttttacgctt cacgcggcga cggcggcggc gatagaaaat ctgaccttcg
     1681 ccaccgactt cagcgataag ctccatccag gctttttgct ctgcgacgca gatatccggg
     1741 ttataactgt cactaagaat gtagacatca aagtgtttgg cattcccggt ggcttttact
     1801 gattcccacg ttgcacgcag gccagcaaaa acacggttca cgtcttcgtt acagataggc
     1861 atgatcaacg ccgtgcgatg ctccgggttt aatggttcat cgccaactgt tgacgcagat
     1921 atactgtatt tatcgcgacc aataagcagt tgcaggaagc ccattaacgc cgtccagaat
     1981 ccggcggaca cccaacagaa cagtaccgca aagaggatca ggataccggt ttgcagcata
     2041 taaggcagaa gctgcataaa ggaaacccac aaatcctgac caaccatatc cataggatta
     2101 atcagcgccc aaccctgata aggaagaatg gtcttcatat accaggtcgc gacgacagtt
     2161 tgcgcgagcg tcaggatcaa cagaatgtaa cggcggatgg taccgacggt acgccacttt
     2221 tgctcgctct cctgctcttc tttggtcaaa cgagccagat agcgcggcgt gacatcgcgt
     2281 ccacgcaggc gatcccagaa acggcctacc gggttggtac gccacgggtc gggaaacatc
     2341 gaggagcgtt ttgcttctgg catcgccttc agctgatcgc gcccttcgtc gtctttaatt
     2401 aactgtccat cagcaagtga atctggccag gcttgttcca gacgcgcctt taccgagcct
     2461 tgcggggaat catcctcccg cgcccaggtg cggtgttcgg catccagcgc ctgatgtacg
     2521 gcgcggatat cagtcttcgg caatgccgct ttctcgcttg cggcgatggg cattgcgtca
     2581 atgtactcag ttgtcttatt cattggcagg taactggtag ctccaggttt cactcaacgt
     2641 ctgatcggca ttcaccagcg cagcacgcat ttcagtggtt ttcttggcat ctttcacttt
     2701 cacacgcatc accagacgcc agcctttggt aaccgggtta tagcgcaccg tgctttcaac
     2761 tatctcacca ttatcaccaa tgctggtttg cgctgtgacc ggggtatcct ctggcagttt
     2821 tttcatctct gcgccggtaa aatcgaccac aaaggcgata gtaccgtcag gctggcgaat
     2881 caggttcgac tgcttcacat cccccgttga acgacgcgtt tgttgcaccc atgcgttatc
     2941 tggcgcatgc agtttgtctt catcacggct gaaggtgatg gtgtatttaa agttcatctc
     3001 tttacccggc tccggcagct gatccggcgt ccagtaagcg acgatgttat cgttggtttc
     3061 atcgttggtt ggaatttcca ccagctcaac gctgccttta ccccactccc ctttcggagt
     3121 cacccatgcg cttggacgaa gatcgtaacg atcatcgaga tcttcaaagc gggagaaatc
     3181 acgaccgcgc tgcaacagac cgaagccttg cgggttctcc atcgagaagc tgctaaccgc
     3241 taaatgtttc gggttattca acggacgcca gatccattcg ccattaccgg catggataga
     3301 gagaccgtta gagtcgtgca actccggacg atagttattt gcaggcgacg gttggttcgg
     3361 cccaaacagg aacatactgg ttaacggtgc accccccagt ttgccgactt tatcgcgcag
     3421 atagattttc gactgcacat ccacaaccgt gtcacgccct ggcataacca cgaatttgta
     3481 agcacctgtc gcgcgcggcg agtcaagcaa tgcataaatg gttaaacgtt tatcagtcgg
     3541 ttttggacgc tcgatccaga actctttgaa gcgtggaaat tcttcacccg atggcaaggc
     3601 ggtatcaatt gccaggccgc gggcagaaag gccataaacc tgacctgcac caatcacgcg
     3661 gaaatagctg gccccgagca tgctgacgat ttcatcgttt ttatctttgc tgttgatcgg
     3721 gtaaagcact ttaaaaccgg caaaaccaag gtcttttacc gtgtctttgt catgctgaac
     3781 atcgccgaaa gtgaaataat ccgggctgta tttgattcgt ttgactgcgg tggcagtcac
     3841 ttcatttatt ttgaccgggg tatcgaagta cataccctga tggtagaact cgagtttgaa
     3901 tggggtcttc agattgttcc agtacgcttt gtcatgatta aactggatct gctgatagtc
     3961 cgcgtatttc atatcgcgga aaacggaggg caagttgctt ttgggcgcct catagccttt
     4021 cccggctaag gattgagctt gctttgcgac atcatcaata ctgaaagccc agcttgaaga
     4081 tgtatacagg gttaacatta ctgcagcact caaccaacgc attttcatca tttgtagttt
     4141 atgtttcata attagtaagc acttccccct ttgtgtgctt atatcgatcc gatctatttt
     4201 aatggataac cagccattcc gacaacagaa tcccggtatc gttcagcacg ctggctcatg
     4261 gattaagcac ctgattgcaa ccgttttcac tttccgtgct tctcctaaag acagaagcat
     4321 cgacttcgtg tagggtttgc cagcaatttt atttatcaac cgtcttacag acgcttccat
     4381 tctggttagt ggtagcgatt atgctatgca taaaaacctt gaagcctgcg cctggcgcgg
     4441 tcaggtcggt gtcgctatgg caacaagcct aaaggcataa cccgacaggg ataagacgaa
     4501 aaatccgaga ttagttaacc atatatgaat ccagtacccg cgcaacgtga atatttcctc
     4561 gactccatcc gcgcctggct gatgttgtta gggatccctt ttcatatttc tttaatctat
     4621 tcgagccata catggcatgt gaatagcgcc gaaccgtcat tgtggctgac cctttttaat
     4681 gacttcatcc actcgttccg catgctggta tttttcgtta tatccggcta cttttcctac
     4741 atgctttttt tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga acgtgtaggt
     4801 atcccgatgt taacagccat ccccttactg acattgccgc aatttattat gctgcaatac
     4861 gtcaaaggga aagcggaaag ttggcctggg ctgtcattgt atgacaaata taatacgttg
     4921 gcctgggaat taatatcaca cctgtggttt ttactggtgt tagtagtcat gacgacgctg
     4981 tgcgtatgga tatttaaacg catcagaaat aatttagaaa attctgataa aacgaataaa
     5041 aaattctcga tggtaaaact atcggtgatt tttttatgcc tcggcatcgg ctatgcggta
     5101 ataagaagaa cgatttttat tgtgtatccg cccattctga gtaatggcat gttcaatttt
     5161 attgtcatgc aaacgctatt ttatttgccg ttctttatcc tcggtgcact ggctttcatt
     5221 ttccctcatc ttaaagcctt gtttaccacg ccgtctcgtg gctgtaccct tgcagcagca
     5281 ttggcatttg tcgcttattt actcaaccaa cgctatggca gtggcgatgc ctggatgtac
     5341 gaaaccgagt cggtgatcac catggtcctc ggtctgtgga tggtgaatgt ggtcttctcc
     5401 tttggccacc gtttgcttaa cttccagtca gcgcgggtga cttactttgt taacgcatcg
     5461 ctatttatct atctggttca ccacccgtta acgctgtttt tcggcgcata cattacaccg
     5521 cacatcacct ccaactggct tggttttctc tgtggcctga tattcgtagt agggattgcg
     5581 ataattctgt atgaaattca tttacgcatc ccgttactga agtttttgtt ttctggtaaa
     5641 ccggttgtta agcgtgagaa cgataaagca ccagcccgtt aagccacatt tacaataacc
     5701 attccacggg caatatcgac gccagtctga ccataacccg cttccagaaa ctggtggcgg
     5761 gttctttttt gagaataatc tctttctttg catgacgatc aacccagttg atccgtcccc
     5821 acctgtccag acggagctgc caggccgcat catactggct ctgtataaag cgtttatcaa
     5881 ttaactgtgc cagcgtttcg ctctctatca cgaagcccat ttcagtattg agcaatgtcg
     5941 aacgtggatc gaaattgaaa gagccgataa acaccgtttt accatcgatg ctaaaggttt
     6001 tagcatgcag gctggcaccg gaattaccgg ttatgccgcg atcgtgtaac gtactacttt
     6061 gttcacgcgt cggcttgagt tcatataatt ccacgccata gcggagcaat tttttgcgcc
     6121 agcgcgcgta tccggcatgg acgacagcaa catcgttagc ggcaagagaa ttggttagga
     6181 tcgcaatctt tacccctttt ctcaccatcc gtaagagttg cgccacacct gcgcgtgtcg
     6241 gtacaaaata ggaagagata atatcgatgc gttcactggg tgagcccatg atatcgaaca
     6301 ggcgctgcgg taacagtgaa tgccgttttg ccttgccctc ccctttcgcc ggatcatcac
     6361 ttaataaacg tgttttcgcc cagataagcg gcaacgttcc atcaaccaga tgatttataa
     6421 acggactgga ttccatttgg cgtaagtaac gatgcgtcat ggcatcgtta tgccaggagg
     6481 cgggtaactc gatgcgatcc gccatttcac cttccgggac atccagcacc tgctgtaagg
     6541 gtgaaaccga tttgcaatac cagtagcggg cgaaatcatc ggcaacgtcc tctaccacgg
     6601 gtcctattgc cataacatct aaatccgaaa aaagtggctc ttcccctgcc ccaaaatagg
     6661 catcaccaat atttcgtcct cccaccaagg taaccacgcc atcgacagtg aaacttttat
     6721 tgtgcatacg gcgattgaga cgggaaaagt cggtgatata accaagcgga cgcagcaagc
     6781 gaaacgagaa aggattaaaa agccggactt caatgcgggg atggctgtca agcaagcgta
     6841 aaatatcgtc aagtccgggc gtattgttgt cgtccagcaa caaacggacg cgaacgccac
     6901 gctttgctgc gactaacagg gcggaaaaca gtaaccgacc cgacatatcg tcctgccaga
     6961 tgtaatactg aacatccagc gtatgctctg ccatttcggc cagacgatac cgggcggcaa
     7021 acgcatccag acttttctcc agagggaaaa gaccacactg accgggatgt tgcgaacaca
     7081 gtggcagcac cgcgctcgcc agccggggca aatcattcat ctccttgttg ggtaaggagt
     7141 ctttcgtaga ggtgggcgtt ttcttcatca taacagacaa agtatacctg ttcgggtaaa
     7201 gcgtgacggg taataaattc tgaaacggtt tttactgcga tttcagccgc tgccgcacga
     7261 gggtaaccat aaaccccagt actgattgca ggaaatgcca ctgacgtata gctgtttgcc
     7321 gccaccagtc gcagactatt gagataggca tcctgcaaaa gctggtcttc gttttgttca
     7381 ccaccacgcc agactggccc gacggtgtgt actacggctt tagcgggaag atcgcctgca
     7441 agcgtaataa cggcatgccc cgtagggcaa tcgccctgct gttgcctgac ttttaaacag
     7501 gcatccagca gggccggacc cgctgcgcga tgaatggccc catcgacgcc gccgcctccc
     7561 attaatgacg gattagccgc attcacaatc acatcaacgg ccagtttggt aatatcaccc
     7621 tgcacaacat gaatacgcgt tttcataacg cctccttaac accttcttgt ttcttaagtg
     7681 tacagcaggc tttttggtta aatcgcattt ttctactcag caacgtgcgt tgttcagaaa
     7741 cttagttata ttcgatgttc attacagcca gctttttctg tttatcaata tactgcactt
     7801 ttactgaggc tacttgcgcg tttttgacat tgccgctcat taacgctttg ctggaatagg
     7861 tttttcccgg aacagaacca ttctgtgggc agttcatttc tacgggtgat gactgggtgc
     7921 tgacgtcaca tgccggttcc acaatttggc cataaaaatg gatcacgccc ccttgcgcgg
     7981 tgcttcctgc gatggcaata aaaggaaaaa aaaactgccg agcataagag agtttaaaaa
     8041 aggtctgaac atttattcta tttcctcaat gattagtcat ccttgagggt tgtgttatcc
     8101 atactttcag gttggctgat tcgcagcaga ccattctctc cagattcatc ttatgctcga
     8161 catttcaaca aattaagact tttctgaaga gggcggccat tgttgtgata a
//
LOCUS       DAJVOY010000174         8162 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVOY010000174 DAJVOY010000000
VERSION     DAJVOY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.174"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..783)
                     /locus_tag="OQL90_003440"
     CDS             complement(<1..783)
                     /locus_tag="OQL90_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1370096.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="OQL90_003441"
                     /pseudo
     CDS             875..961
                     /locus_tag="OQL90_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1262..1351
                     /locus_tag="OQL90_003442"
                     /pseudo
     CDS             1262..1351
                     /locus_tag="OQL90_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1375..5889
                     /locus_tag="OQL90_003443"
                     /pseudo
     CDS             1375..5889
                     /locus_tag="OQL90_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            5890..7539
                     /locus_tag="OQL90_003444"
     CDS             5890..7539
                     /locus_tag="OQL90_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1370097.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7544..>8162
                     /locus_tag="OQL90_003445"
     CDS             7544..>8162
                     /locus_tag="OQL90_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370098.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQV"
BASE COUNT         1993 a         1964 c         2290 g         1915 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggt aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatgtt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgctcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccactgcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcaactaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga agacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 gctaagtagt tgattcatag tgactgcata tgttgtgctt tgtcgcattt tgtagtctat
     1201 tatttaatct gtaacaacga taatacaatg aattttaatt ggtttttgtg attttgtcct
     1261 tcttctgaca tggaaggaca cccgatggat acccaacgat tccaatccca atttcattgg
     1321 catttatcgt ttaaattttc tggcgcgata gcagcgtgct tgtctctgtc tcttgtggga
     1381 acagggcttg ctaatgctga tgattcgctt ccttccagta actatgcgcc gcccgccggg
     1441 ggaacattct ttttgcttgc tgacagcagt tttggcagca gtgaagaggc gaaagtgcga
     1501 ctggaagcgc cggggcgtga ttatcggcgc tatcagatgg aagagtacgg cggcgtggac
     1561 gttcgcctgt atcgtattcc tgacccgatg gcatttttgc gccagcagaa aaacctgcat
     1621 cgcattgtgg tgcaaccgca atatctgggc gacgggctga acaatacgct gacctggctg
     1681 tgggataact ggtacggcaa atctcgccgc gtaatgcagc gtactttctc ttctcagtca
     1741 cggcagaatg tgactcaggc attacccgaa ttacatctcg gcaatgccat tattaaacct
     1801 tcccgttatg tacagaacaa ccagttttcc ccgctgaaaa aatatccctt ggtggaacag
     1861 ttccgttatc cactatggca ggctaaaccg gtcgagccgc agcaaggggt aaaactggaa
     1921 ggcgcatcca gcaatttcat ctcgccgcag ccgggtaaca tttatattcc tctcggccaa
     1981 caagagccgg gactgtacct cgtcgaggcg atggttggtg ggtatcgggc gacgacggtg
     2041 gtgtttgttt ccgataccgt ggctcttagc aaagtgtcag gcaacgagct tctggtgtgg
     2101 accgcgggta aaaaacaggg tgaagcgaag cccggctcag agatcttgtg gactgacggt
     2161 cttggcgtga tgacccgcgg tgtgaccgat gacagcggta ccttgcagtt acaacatata
     2221 tcgccagaac gttcatacat tctgggtaag gatgctgaag gcggcgtttt tgtctccgag
     2281 aacttcttct acgaaagcga aatctacaac acccgcttgt atatttttac cgatcgcccg
     2341 ctatatcgcg caggcgatcg tgtcgatgtt aaagtgatgg gccgcgagtt ccacgatccg
     2401 ttgcattcat cccccatcgt cagcgccccg gcgaagcttt cggtgctgga cgctaacggc
     2461 agtctgttgc aaaccgtcga tgtcacgctg gatgcgcgca atggcgggca gggaagtttc
     2521 cgcctgccag aaaatgccgt tgccggaggt tatgagttac gtcttgctta ccgcaatcag
     2581 gtctatagca gcagttttcg cgtggcaaac tacatcaagc cacatttcga gattggttta
     2641 gctctcgaca aaaaagagtt caaaactggc gaagcggtca gcggcaaact gcaactgctc
     2701 tacccggatg gcgagccggt aaaaaatgcc cgcgtgcagt taagtttgcg cgctcagcaa
     2761 ttatcaatgg tcggtaacga tttgcgttat gccggacgtt tccccgtgtc gctggaaggc
     2821 agcgaaacgg tgtccgacgc cagcggtcat gtggcgttaa atctccccgc cgccgataaa
     2881 ccgagccgtt atttgttaac cgtctccgcc agtgacggcg cggcgtatcg tgtcaccacc
     2941 accaaagaga tcctcattga acgcggtctg gcgcattact cattaagtac tgccgcacaa
     3001 tacagtaata gcggcgagtc ggttgtgttc cgttatgccg cgctggaatc ttcaaaacag
     3061 gttcctgtta cgtatgaatg gttgcgtctc gaagaccgca cgagccatag cggagagcta
     3121 ccgtcaggcg gcaaatcctt taccgtcaat ttcgctaaac ctggcaacta caatctgaca
     3181 ttacgcgata aagacggctt aattctcgct gggttaagtt atgccgtcag cggtaagggc
     3241 agcacggcgc atacgggtac ggtagatatc gtggcggata aaacgctgta ccagccaggc
     3301 gaaaccgcga agatgctgat tacctttccg gagccaattg atgaagcatt attgacgctg
     3361 gaacgcgatc gcgtggaaca gcagtcgctg ctttcgcatc cggcaaactg gctaacgcta
     3421 caacgtttaa acgataccca gtatgaagcc cgggttccag tgagcaattc ctttgcgcct
     3481 aacatcactt tttcggtgct gtatacccgt aatggtcagt acagttttca gaacgccggg
     3541 atcaaagttg ccgttcctca gcttgatatc cgggtgaaaa cggacaaaac ccattaccag
     3601 ccaggtgaac tggtcaatgt cgaattaacc tcgtcgctga aaggtaaacc tgtttctgcg
     3661 cagctaacgg taggcgtggt cgatgaaatg atctacgcgc tgcaaccaga aatcgcgccg
     3721 aatatcggca aatttttcta tccgctgggg cgtaacaatg tgcgtaccag ctccagtctg
     3781 tcgtttatca gctacgacca ggcgctctcc agcgagccgg ttgcgcctgg cgcaactaac
     3841 cgcagcgagc ggcgagtaaa aatgcttgaa cgtccacggc gtgaagaggt ggataccgcg
     3901 gcatggatgc cgtcactcac aaccgataaa caaggcaaag cgtatttcac gttcctgatg
     3961 cctgattcgt taacccgctg gcgtatcacc gcgcgtggga tgaacggcga cgggctggtc
     4021 gggcaggggc gtgcttatct gcgttcggaa aaaaatctct acatgaagtg gagtatgcca
     4081 acggtgtatc gcgtgggcga caaaccggcg gcaggactgt ttatcttcag tcagcaggat
     4141 aacgaaccgg tggcgctggt gactaaattt gcaggcgctg agatgcgcca gacgctgacg
     4201 ctgcacaaag gggcgaatta tatttcgctg gcgcagaata ttcagcaatc tggcttgtta
     4261 agtgcagaac tgcaacaaaa tgggcaagtg caggacagca ttagcacaaa actgtctttt
     4321 gtggacaaca gcaggcccgt tgaacagcag aaaaatgtca tgctcggcgg tggcgagaac
     4381 gcgctgatgt tgcccgagca ggcgagtaat atccggctac aaagtagtga aacgccgcag
     4441 gagattttcc gcaacaatct tgatgcgtta gtcgatgaac cgtggggtgg ggtgatcaac
     4501 accggtagcc gtctgatccc gctcagtctc gcctggcgtt cgcttgccga tcatcaaagt
     4561 gccgccgcta acgacattcg tcagatgatt caggataacc gtctgcggct gatgcaactg
     4621 gcggggcccg gagcgcgctt tacctggtgg ggtgaagatg gcaatggtga cgccttcctt
     4681 acggcatggg catggtacgc cgactggcag gccagctagg cgctcggcgt aacgcaacaa
     4741 ccggaatact ggcagcatat gctcgacagt tatgccgagc aggcggataa catgccgtta
     4801 ttgcatcggg cgctggtgct ggcgtgggca caggagatga atctgccgtg caaaacgttg
     4861 ttgaaagggt tggatgaagc tatcgcccgg cgcggaacta aagatgaaga tttctctgag
     4921 gaagacatcc gcgatatcaa tgacagcctg atcctcgata caccggaatc tccactggca
     4981 gatgcggtgg caaacgtctt aaccatgacg ttgctgaaaa aagcgcagtt gaagtccacg
     5041 gtgatgccac aggttcagca atatgcgtgg gataaagcgg caaacagcaa tcagccgctg
     5101 gcgcacacgg ttgtgctgct caatagcggt ggtgacgcta cccagacggc cgctatttta
     5161 agtggtttga ccgctgagca atccactatt gagcgcgcgc tggccatgaa ctggctggcg
     5221 aaatatatgg cgacaatgcc tccagttgtt ttgcctgcgc ctgcgggcgc atgggctaaa
     5281 cataagttaa ctggaggggg cgaatactgg cgttgggttg gtcagggcgt gccggacatt
     5341 ctctcttttg gtgacgaatt atcgccgcaa aatgtgcagg tccgctggcg tgagccggca
     5401 aaaatggctc aacaaagtaa cattccggtg accgttgaac gccagttgta tcggcttatc
     5461 cctggtgaag aagagatgag ctttattctg caaccggtga ccagcaatga gattgacagc
     5521 gatgcgctgt atctcgatga catcacgctt accagcgagc aggatgcagt tctgcgctac
     5581 ggtcaggtgg aagtaccgct gccaccggga gccgacgttg agcgcacaac atggggcatt
     5641 tcggtcaata aacccaacgt cgcgaaacag caggggcaat tgctggaaaa agcgcgaaat
     5701 gaaatgggcg aactggccta tatggtgccg gtgaaagaac tgacgggaac ggtcactttc
     5761 cgccatttgc tgcgcttctc gcaaaaaggg caattcgttc tgcctcctgc tcgttatgtg
     5821 cgttcctatg cacctgcaca gcaaagtgtt gcggcaggga gtgaatggac cgggatgcag
     5881 gtgaaataag tgaactggcg cagaatcgtc tggctattgg cgttggttac tcagccaacg
     5941 cttgcggagg agacgccgtt acaactggca ctgcgtggtg cgcagcacga tcaactgtat
     6001 cagctgtcgt catcaggcgt gaccacagtc tcagcattac ctgactcgct caccacgccg
     6061 ttagggagtt tgtggaaact gtatgtttac gcctggctgg aggataccca tcaaccggag
     6121 cagccctacc agtgtcgcgg aaactccccg gaagaggttt attgttgtca ggctggggag
     6181 agcataacgc gcgataccgc gctggtacgt tcctgtgggc tctattttgc gccgcagcgg
     6241 ttgcatattg gcgcagatgt gtgggggcaa tactggcaac agcgtcaggc gcctgcgtgg
     6301 ttagcgtctc ttactacgct gaagccagaa acctcggtaa cggttaaatc gctgcttgat
     6361 tcattagcga cgttacctgc gcaaaacaaa gctcaggaag tcttactgga tgtggtgctg
     6421 gacgaggcga aaatcggtgt cgcatccatg ctcggtagtc gggtacgggt gaaaacctgg
     6481 agctggtttg ccgatgataa acaggaaatc cgtcagggtg gttttgccgg ctggttaacc
     6541 gatggtacac cgctgtgggt tactggcagt ggcaccagca aaacggtact aacccgttat
     6601 gcgacagtgc ttaatcgggt tttgccagtg ccgacgcagg ttgccagtgg gcaatgtgta
     6661 gaggtggagc tgtttgcccg ctatccgctg aagaaaatca cagcggaaaa gagtacgacg
     6721 gcagtaaaac ccggcgtact taatgggcgc tatcgtgtga cgtttactaa tggtaaccac
     6781 attacgtttg tcagccacgg tgagacgacc ttactgagcg aaaagggcaa actgaaattg
     6841 cagtcgcatc tcgatcgtga agagtacgtt gccagagttc tggatcgcga ggcgaaaagt
     6901 acgccccctg aggcagcgaa agccatgacc gtggcgattc acactttttt acagcaaaac
     6961 gccaatcggg aaggcgattg cctgaccatc cccgacagca gcgccacgca gcgtgtttct
     7021 gcttcgccag cgaccacggg ggcgcgaacg atgaccgcct ggacgcagga tctcatctat
     7081 gcaggcgatc cggttcatta tcatggcagt cgagctaccg aagggacact ttcctggcgg
     7141 caagccacgg cccaggcggg gcagggagag cgttacgacc agatactcgc gtttgcttat
     7201 cccgacaaca gccttagccg ctggggagcg ccacgctcaa cctgccagtt attacccaaa
     7261 gcgaaagcct ggctggcgaa aaaaatgccg cagtggcggc gtatattaca aggcgagacg
     7321 gggtacaacg aaccagacgt gtttgcggtc tgtcgtttag tctcaggttt cccctatacc
     7381 gatcggcagc agaaacggct gtttattcgt aacttcttta cgcttcagga tcggctggat
     7441 ttaacccatg agtatctgca cctggccttt gatggttatc ccaccgggct tgatgagaac
     7501 tatatcgaaa cgctgacccg acaattatta atggactaat gctatgcgaa agatttttct
     7561 tccgttgtta ctggtggcac tttcgcctgt cgctcacagt gaaggtgtgc aggaagtcga
     7621 gattgacgca ccgctctctg gctggcatcc ggcagagggc gaagatgcca gtttttcaca
     7681 atcgatcaat tacccggcgt cgtccgtcaa catggcagat gatcaaaata tctcagcgca
     7741 gatccgcggc aaaataaaga attatgctgc ggcgggtaaa attcagcagg gccggctggt
     7801 ggtcaacggt gccagtatgc cgcagagagt tgaatccgat ggttcatttg cacgccctta
     7861 tattttcact gaaggcagca acagcgtgca ggtcatcagc ccggatgggc aaagccgaca
     7921 aaaaatgcag ttttactcaa cgccgggtac aggaacgatt cgtgcacgtt tacggctggt
     7981 tctctcgtgg gatacggaca ataccgatct cgatctccat gttgtcacgc ccgatggcga
     8041 acacgcctgg tacggtaaca ccgtgctgaa aaacagtggt gcactggata tggatgtcac
     8101 gacggggtac ggacccgaga ttttcgccat gccagcgcca gttcacggac gctatcaggt
     8161 gt
//
LOCUS       DAJVOY010000175         8140 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVOY010000175 DAJVOY010000000
VERSION     DAJVOY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.175"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..263
                     /locus_tag="OQL90_003446"
     CDS             <1..263
                     /locus_tag="OQL90_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1370099.1"
                     /translation="AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFR
                     FYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(402..1031)
                     /locus_tag="OQL90_003447"
     CDS             complement(402..1031)
                     /locus_tag="OQL90_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1370100.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1154..2800)
                     /gene="fumB"
                     /locus_tag="OQL90_003448"
     CDS             complement(1154..2800)
                     /gene="fumB"
                     /locus_tag="OQL90_003448"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1370101.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRI
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2878..4218)
                     /gene="dcuB"
                     /locus_tag="OQL90_003449"
     CDS             complement(2878..4218)
                     /gene="dcuB"
                     /locus_tag="OQL90_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1370102.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4789..5508)
                     /gene="dcuR"
                     /locus_tag="OQL90_003450"
     CDS             complement(4789..5508)
                     /gene="dcuR"
                     /locus_tag="OQL90_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1370103.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5505..7136)
                     /gene="dcuS"
                     /locus_tag="OQL90_003451"
     CDS             complement(5505..7136)
                     /gene="dcuS"
                     /locus_tag="OQL90_003451"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1370104.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7317..7547
                     /locus_tag="OQL90_003452"
     CDS             7317..7547
                     /locus_tag="OQL90_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCS1370105.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7559..7831
                     /locus_tag="OQL90_003453"
     CDS             7559..7831
                     /locus_tag="OQL90_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1370106.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8058..>8140
                     /gene="ghoS"
                     /locus_tag="OQL90_003454"
     CDS             8058..>8140
                     /gene="ghoS"
                     /locus_tag="OQL90_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1370107.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRL"
BASE COUNT         2022 a         1967 c         2082 g         2069 t
ORIGIN      
        1 ttgcggttgt gttagggatg attggctatg taccgaatgt tgaacagtct acgcaagccc
       61 tattaggtat gcagtttatt atgattgctc taccaactct gtttttcatg gtaacgctga
      121 ttctctactt ccgtttctat cgcctcaatg gtgacacgct gcgcaggatc cagatccatc
      181 tgctggataa atatcgcaaa gtaccgcccg agcctgttca tgctgatatt ccggtcggtg
      241 cagtgagtga tgtgaaagcc tgacgtggac aatagctaac gaaaatggcc tgatgcgata
      301 tgtttatcag gccacgtctt aatgtaatgc tttggatttg caaattttgt agaccggata
      361 aggaattcac gctgcatccg gcatcaacaa agcgcacgtt gttatccggt tatcaagcca
      421 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
      481 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      541 tcatccgctc cctgacccat tgccagcgcc gcccaccact cgattaattc atacattgcg
      601 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      661 atcccgcgaa cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaagtgc
      721 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccaacg
      781 gggactttcg cgtaggtgta ttgtccgccg accatcagga tgatggcgtg aaggaaaatg
      841 agcgtataga gcagcggcgt taacggataa cgtctggcgg tggcaagcag tagcggcacg
      901 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      961 gtataaatca ggattaatgt tagcgccagt gcgccggtgt taagaattaa cggcttgagt
     1021 gtgcgggtca tggtgagttc gcaaatcagg gaaaatactg actattcacc agcaagcgtg
     1081 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
     1141 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
     1201 agtcgttacc tttgtcatcg accaagataa acgccgggaa atcttctact tcgattttcc
     1261 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
     1321 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
     1381 acgtgtcggt aacctgctga ctgcggttac ctttcgccag catgatcatg ctgccgccgt
     1441 gggattgcag cagatccacg taggagtcca tacggcctgc ggtggttggg ccaagtgaac
     1501 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1561 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1621 ctcggcccac gataatggtg ccggtgagcg acaaacgagt ggataccggg tattgcgaaa
     1681 gctgggcgag gatctctttc atcgggcggt taaggtcaac tttcaccgct tcgccttcac
     1741 cggcctggcg cagttcttgt ggaatgtact ggcctgggtt gtgttccagt ttttcgatcc
     1801 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1861 tgccgaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1921 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1981 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     2041 cgtaatagtg agcgcttgct aacttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     2101 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgaggata cgcattttct
     2161 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     2221 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     2281 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     2341 tgtccagcgc cgcattctgt gaatagcgca ggttatcttc gatataggtg ttatagacgc
     2401 ctttcgacag cgcttcttca tcaccgccgc cggtccacac gcgctggcct tttttaccga
     2461 cgatgatcgc gatgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2521 aatttcttaa gaattgcagc gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2581 gaatagccgc aacctgtttc tggtgtgccg ggcggagcat aaaagaagcg tcgtgaaagg
     2641 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2701 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2761 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2821 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2881 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2941 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     3001 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     3061 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca acgcgaccgg
     3121 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     3181 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     3241 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     3301 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     3361 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3421 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3481 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttatccag
     3541 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3601 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3661 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3721 accatcaaag gtgacattac ccagcatcgc aaccagagac acgaccgcaa ccgacaccgg
     3781 actggcgata atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3841 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3901 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3961 cgggttgcgg cgcagcaact tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     4021 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     4081 tttacctggc tgaaggtgga agacaaagac cagaatgacc agaccgatac cgcctaataa
     4141 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     4201 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     4261 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     4321 tggtcgttac cggctttagc aaatacctca cagtaaatat tggctgatta aagcagcggc
     4381 aaatttatgc actgtgataa gcggcttttt cagagagaaa aactctctgg cgatgggttt
     4441 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4501 gtctattttt tgttctcagg gcagatatca ttaggtttaa agattaatct tatctaccca
     4561 tttgtgggtg aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4621 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatggaggg
     4681 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4741 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4801 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaaccg gacgccccgt
     4861 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4921 acggcaggaa acacgcgaga tgttaacctc gttagccagc tcgtcggttg aaaactcata
     4981 gtcctgatgc gcgtcaatcc actggcacag cgtgcgtaac gtctgcggcg ttaagccttt
     5041 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     5101 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agccggtgag
     5161 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     5221 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     5281 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     5341 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     5401 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5461 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgctcatct gtttgacctc
     5521 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5581 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5641 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5701 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5761 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5821 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag caggctttca
     5881 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcggt taatcttgct gattaaaaaa
     5941 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc cttatagtta
     6001 ttggctgttt tgagactgta atcttccaac tgcttataac tcttcagatg caataatccg
     6061 agaatcacat gcaatttatt cataaattcg tgggatcgtt cacgaagtgc atcagcatag
     6121 ttgaccagac cgtcgagtcg ctgcatcagt ttacgaactt cagttttgtc cctgaaggtt
     6181 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     6241 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     6301 acatctacca cctgtgacca tgagtggctt agcgtcgaca gtttctcatc gtcctgcgac
     6361 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6421 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgctg ctcaaacagc
     6481 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6541 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6601 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgctcaccac cacgccaatt
     6661 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6721 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6781 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atccgtaacg
     6841 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gattccgctc
     6901 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agtcggcgag ggtacgcgcc
     6961 actgccagtg ccttgttggc cagcccgtct cgtgtcatat cactgatttg cgagaagtaa
     7021 atcagatgca ccaccaatag caccgagaac tgtaccgcgc tgaccattaa gatcactgtg
     7081 gtactcaatt tcatcggacg tttgagtaac atgcggtagg gcaatgaatg tctcatcagc
     7141 ttccttgtgt gacaaatttc ttaagcatta tctctgatga ggcgggtaat tcaaagggag
     7201 taagaatgat tggctatata ggggaagaga ctctggcaac ggaaactgcc agtgctgtat
     7261 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     7321 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     7381 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7441 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgtcact gtggttaaag
     7501 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7561 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacagggca agcaaatcgc
     7621 tgaaattgtc tttgtgccga ccggagagaa tttagcgatt atcgaacata ccgatgtcga
     7681 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcgaaagtcg tggaaaaaat
     7741 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7801 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7861 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7921 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7981 ctcctatatg agaatcatca atcggggtta ataagttttg cgtccccaga gcattcaata
     8041 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     8101 attatccatc atcttattcc tcagtgttct taaggttaag
//
LOCUS       DAJVOY010000176         8047 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVOY010000176 DAJVOY010000000
VERSION     DAJVOY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.176"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..1510)
                     /gene="hscC"
                     /locus_tag="OQL90_003455"
     CDS             complement(<1..1510)
                     /gene="hscC"
                     /locus_tag="OQL90_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCS1370108.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFK"
     gene            complement(1594..2529)
                     /gene="rihA"
                     /locus_tag="OQL90_003456"
     CDS             complement(1594..2529)
                     /gene="rihA"
                     /locus_tag="OQL90_003456"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1370109.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2647..3372)
                     /gene="gltL"
                     /locus_tag="OQL90_003457"
     CDS             complement(2647..3372)
                     /gene="gltL"
                     /locus_tag="OQL90_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1370110.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3372..4046)
                     /gene="gltK"
                     /locus_tag="OQL90_003458"
     CDS             complement(3372..4046)
                     /gene="gltK"
                     /locus_tag="OQL90_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1370111.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4046..4786)
                     /gene="gltJ"
                     /locus_tag="OQL90_003459"
     CDS             complement(4046..4786)
                     /gene="gltJ"
                     /locus_tag="OQL90_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1370112.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(4956..5864)
                     /gene="gltI"
                     /locus_tag="OQL90_003460"
     CDS             complement(4956..5864)
                     /gene="gltI"
                     /locus_tag="OQL90_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1370113.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6319..>8047
                     /locus_tag="OQL90_003461"
     CDS             6319..>8047
                     /locus_tag="OQL90_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCS1370114.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYV"
BASE COUNT         2026 a         1970 c         2013 g         2038 t
ORIGIN      
        1 ctttaaaggt gcgattgatg agcatatcgc gcggataaat tttcaatgcg gataaccgcg
       61 tccgactctc ttcaatctgc tgcgcgctca atgttaccgg gctgtggtta atcactctgg
      121 acttaacgct gccgtcttcc agaagcacgt caacttcaag caacccgttg atatcataac
      181 taaagcgaat atcaatcgac tgataagccc cggttttctt caacggcaca tcgaaggatt
      241 caaccagaat gttattttta actttgtggt tttctccctg atagacgtta accgtaattg
      301 aatcctgctc cgggtgcatg gtcgagtaag tttctacacg cgacaccggt actgtggtgt
      361 ttcgttcaat aatcggcgag aaaatgccgg aaacgccctg gcggttaact tcaacgccca
      421 acgagtaagg gcaaatatca gtgaggatta cctcttcaat atcttcactg cgtaagcggc
      481 aggcggcctg gattgctgcg cccagcgcga caatggtgct cggatcgtaa ctttgatacg
      541 gtaatttgcc aaacagacgc acggcgattc gctgcaccag cggcatttgt gacgcgccgc
      601 caaccagcac cagactgtcg atttgactcg gcttcagacg cgcatcgcgc aacgcctgtt
      661 caatcggcac tcgcaagcga ttgagcagcg gcaaccacaa atcttccagt tcgttctcgt
      721 aaaattcgca ttcccgcgtt tcttcctgat actgccagcg aatgtgcaat ggcgattgat
      781 tgctacattt tgccgcttcc acacaggcgt acaaggctgc cagttcactc tcgttaagcg
      841 tggtcctggc aacttccgcg cgttttaaaa cctcatcgac cagcatatgg gtaaaatctt
      901 cgccaccgag gaagctatcg ccagcggagg cgtgaatttc aatcaccggc gtggcgtact
      961 caagcaccgt aacgtcaaac gtgccgccac cgagatcaaa aaccagcgag cgggtatttt
     1021 gttgcgtatg caggccatat gccatcgcag ctgctgtggg ttcattaatt aagcgtaccg
     1081 catttaaccc ggctaactcc gcagctaaac gggtatgctt gcgttgttca tcgctgaaat
     1141 aagccggaac ggagatcacc acatctttaa tcggacgttg cagaaattct tcggcatctt
     1201 cttttaatga gcgtaatacc aaagaggaca gttctggcgc gttaaatgtg tcgctgccta
     1261 accgccagtt ggtattactg cccattgcgc gtttaaataa cgctgccgtt ttatccggat
     1321 gcgaagtacg ccgtgataca gccggttttc cgactaaaat atgattattt tcatccatgc
     1381 taattatgga tggtgttaaa tattcaccga acttatttgg aattaattgc gcggcaccgt
     1441 ctttccagac ggcaattaaa ctattggtag tgccgagatc gataccaatg gtgagttctg
     1501 cattatccat tgcatttata tcttgaaaga aaaagagata aacagattaa gaccacccag
     1561 gtaacgcgtg gtgatcttaa tcaatgacgt gtgttaagcg taaaatttca gacgatcagc
     1621 cagtaaatca acaaagccct gacgatcaac atcgaccatt acagtggcat tcggtttatt
     1681 gtcagtcaga taataataat caacaaccgt cataccctgg gtatatttcc cctgtgtttc
     1741 cacgccaacc cagcgctcaa cagtggtaaa caactcgggt ttcaacagcc aggcgatggt
     1801 gcatgggtca tgcagcggtg caccgacaaa gccccatttt tcgtctttat gatattcgag
     1861 gaagaaatcc agcagttcgg caacaatggt tgaaacaggg ttgccaatcg cgcggaaacg
     1921 ctcggtgtct tcaatgtgga tttgcgcttt atgagtaaca tccagaccgg ccatcaccac
     1981 tgggatccct gactggaaga caatttctgc cgcttccggg tcaacgtaaa tattaaattc
     2041 agccgcaggc gtccagttac caagccccat tgcgccgccc ataatcacga tacgggcaat
     2101 tttgctatgc agttccggat ggctattgag cagcaaggca acgttagttt gcggtccggt
     2161 agacacaatc gtgacaggtt ccgcactttc gcgcagcgtt ttcgccatca gctctaccgc
     2221 cgtacagttt tgcggtgcga acgtcggttc cggtaatgcc gggccatcca gaccgctttc
     2281 gccgtgcaca ttgtccgcga taatcaactc acgcattaac ggttttaccg cgccgcctgc
     2341 taccggaata tcggtgcgat taagcaaggt cagcatacgc agaacattgc gtaaggtttt
     2401 ttctggtgtc tggtttccgg cggaagacgt aattgctttg acatcaagct ctggtgaggc
     2461 gagggcgaga actattgcga tagcgtcgtc atgacctggg tcgcaatcta acagaattgg
     2521 cagtgccatt gttgctcctt gttgtgtgct tctttgcgac aagggtaacg ccaggatgta
     2581 acagatacga ggggcgaaac gataaagcgt gagatggcgc gcacttgggt atgcgcgcca
     2641 gagtgattaa tgcaggattt tcgcgaggaa gtctttcgcg cggtccgatt tcggatcatc
     2701 gaagaaagcg tctttcggcg agtcttcgac aattttaccc tcgtccataa agatcacccg
     2761 attcgccact ttgcgggcaa agcccatttc gtgggtcacc accatcatgg tcattccttc
     2821 gttcgccagt tccaccatca cgtccagcac ttcgttgatc atctccggat ccagcgccga
     2881 tgtcggttcg tcgaacagca tcgcaatagg atccatacac aacgcgcgag cgattgccac
     2941 acgctgctgc tgaccgccgg aaagctgcgc cggaaattta ttggcatgtg cggaaagtcc
     3001 cacgcgctcc agcaatttca atgctttttc acgagcgggc gctttatcgc gtttaagcac
     3061 tttcacctgc gccagagtca ggttttcaat aatcgacaaa tgggggaaca actcaaaatg
     3121 ctggaacacc atcccgacgc gggaacgcag ctttgccaga tcggttttct tgtcattaac
     3181 cacgatacca tcgacggtga tttcaccttg ctgcactggt tcgaggccgt tgacggtttt
     3241 aatcagcgtt gatttgccgg aaccagacgg gccgcaaacc accaccactt cgcctttttt
     3301 cacttcggtt gagcagtcgg tcagcacctg aaagtgacca taccattttg aaacattttt
     3361 cagggtaatc attatgctgt ccttcttttc aagtagctga ccaacaacga cgcgctaaga
     3421 ctaataacga aataaacaaa tccggcaaac aggatcatct caacctgcgt accatcacgc
     3481 tcaccaatgg ttgaggcggt acggaagaaa tcggccagag ataacacata caccagtgag
     3541 gtatcctgga acagtacgat gccctgagtg agcagaagcg gcaccatcgc gcggaacgcc
     3601 tgcggcagaa taatcagttt catcgactgc cagtgagtca ttcccaacgc cagcgcggcg
     3661 ctcgactgac cacgagaaat actttgaata ccagcacgga taatctctga atagtaggcc
     3721 gcttcaaaca tcgaaaacgc caccatcgcc gaaattaaac ggatatcatt ttttggtgat
     3781 aatcccagca cgttttgcag aaaacctggc acgatcaggt aaaaccacag caaaaccata
     3841 actaaaggaa tcgagcggaa tatgttaacg taggctttgg caaaccacgc tacgggcgca
     3901 aagctggaca agcgcatcac cgccagcatg gtgccccaca aaataccaat cacgactgcc
     3961 gtgacggtga ttttcagcgt gatcaccagc ccgtcgagca gatatggcag ggaagggaca
     4021 atggaactcc agtcaaactc gtacattatt tgccccccat gttgccaggc aggcgaactt
     4081 tacgttcaac cagcgtcatt accagcatga taatcgcgtt aatcaacaca tacgccagcg
     4141 taatagcggt aaacgactcc caggcgtggg ctgagtaatc aagcaattta cccgcctgcg
     4201 ccgccatatc caccagacca atagtcgagg cgatggcgga gtttttcacc aggttcatca
     4261 tctcggaagt catcggcggg acgataacgc gataagcatt aggcagcagt acgtatcgat
     4321 aagcctgcgg tagcgtcagg cccatcgcca gcgcggcatt tttttgccca cgcggcaacg
     4381 actgaatggc ggcgcgtacc tgttcgcaaa cacgggcggc ggtaaacagc cccaggcaga
     4441 gcatggatga aaggaaaaac tgaatattgg gatccagctc ggccttaaac cacatgccga
     4501 ttttctccgg caacagctcc gggatcacca gataccaggt aaagaattgc acaatcaacg
     4561 gcacgttgcg gaacagttcg acatacaacg tacccagacc ggagagaaaa cggtttggaa
     4621 cggtacgtaa aatgccgaaa aatgaaccga cgaggaaagc gataatccag gcgcagatcg
     4681 acaaagcgat cgtcacctga aaaccactcc agatccaacc aagataggtg gtgttgccga
     4741 acggggcttg ttgtaaaaaa ataccccagt tccagtctat agacataaat ctactccaga
     4801 aaaaagaggg tagcagcgtt aactgctacc ctcgaagatt gttacccagc gtattgcggt
     4861 tttcaggccc gatggggaac gaccatcagg cgtatagtct gtccgtgcta cgtaacaatc
     4921 gagagggctg gaatttccgc ccctggttct tgtaattagt tcagtgcttt gtcattcggt
     4981 tctttgaaca gtgctttcat ttcgtctgac agttcgaaat tcatgttcag gtttttcggc
     5041 ggaattggat ttttgaacca tttatcaaac catttttccg cttcaccgga agtctgcacc
     5101 tgagcgatag tgtcatccat cagctttttg aactgcggat catctttacg caacatgcaa
     5161 ccgtaggctt cctgagactg cggcttgccg acgatatccc agttgtctgg tttcttcgct
     5221 ttcgcacgtt caccggccag cagggcatca tccatcataa aggcaacggc acgaccgctt
     5281 tccagggtgc ggaaagagtc accgtgatct ttggcgctga tgatgcgcat attcattttt
     5341 tgctcttcat tcagtttgtt gagcaaaact tcagaggtag tgccggaagt gacgactacg
     5401 gctttgtctt tcaggtcggc aaaatctttg atatcgccgc cctttttggt cagcaggcgc
     5461 gtaccgacca cgaaaatagt atcagagaaa gccgcctgtt tttggcgttc gacgttgttg
     5521 gtggtagaac cacattcaaa atcgaaagtg ccgttttgca gcagtggaat acggttttgt
     5581 gaggtaatcg gaatcagttt tacctgcaag tccggtttgt tgagtttctt tttcactgct
     5641 tcaacaatgg cgttggagta atcctgcgag taacccacca ctttttgctg attgtcgtaa
     5701 taagagaaag gtactgaaga ttcacggtga ccgacgacaa tcacaccgtt tttggcgatt
     5761 ttgtccagcg tactgcctgc tgccggggcg gcgtcatctg cctgtgccag tcctgcggaa
     5821 agcgccaggg cgaggattgc tgtggcaggt ttacgtaatt gcatatccaa ctcctttatc
     5881 ttctgcgtta agaacgcatg gatacccgtt gtgagtgttt gtgttgttat cgtctgcaac
     5941 tttattgtgc agtgttgtgc ctgttaggta acatttagtt tggctaaatg taaagatatt
     6001 gctgttttat tgtttgtttt tgcgagatgc gccgcaccat tccgaagcaa aattcttaaa
     6061 ccgcaccttt ttggtgctac cgctgatttg tggtggtgca acctggttgc gtcgcggctg
     6121 atttcatcgc ttacacagta aataaagcaa cttacatgcc aaaaaatact taggggcaaa
     6181 ttaattacga ttgtaatttt aggctgaaaa agtcatattt cggacagaat tatcttttcg
     6241 gcatgggacg tttgccggga tgataactgc acacgggaaa aatgcttttt acctgtcttt
     6301 tcaaggaaga aacaccttat gtctgcatct tcggttaagc cgttaaatgt tcaattaccc
     6361 gcaataaccc ttatcctttt tgcactctgt attgggatat tttgttatct cgcacaatgg
     6421 atgagttatg aagaggtcga tcaatccgca cttatccatc tcggcgcaaa cgttgcgcct
     6481 cttactttgt cgggtgaacc ctggcgctta ttgagcagta tctttctgca cagtagtgtt
     6541 tctcatctgc tgatgaatat gtttgcattc ctggtcgtgg gaggcgtggc ggaacagatt
     6601 ctggggaaat ggcgactcct gattacctgg ttattctccg gcgtctttgg tgggctcatc
     6661 agcgcctgtt atgcgttacg cgatagtgat cagatagtca tcagcgttgg ggcatccggg
     6721 gcaattatgg gaatagctgg cgctgcgata gcaacacagc ttgcttcagg tgcgggcaca
     6781 taccataaaa accagcggcg agtattttct ctgttgggta tggtggcgct gacactgttg
     6841 tatggtgccc ggcaaacagg aatagataat gcttgtcaca ttggcggcct gattgcgggt
     6901 ggcgcgttgg gttggctgag cgcgcgttta tctgggcaaa accgactcgt tacggaaggc
     6961 gggattattg ttgcgggcag tcttcttctg accggggcta tctggcttgc gcagcagcag
     7021 atggatgagt cagttttaca agtcaggcaa agcctgcgtg aagcgtttta tccacaggag
     7081 attgaacaag agcgacggca aaaaaagcag cagttagcgg aggaacgcaa cgccctcagg
     7141 gaaacattat ccgctccggt aagtcgtgaa caggccagtg gtgatttgct cgctgagatt
     7201 gccgatatcc atgatatggc gatcagtcgg gatggtaata tgttgtatgc cgcaattgaa
     7261 aacacgaaca gcattgttgt tttcgacctc ggacaaaaga aaatcctgca tacctttaca
     7321 gcgcccatag cgaaagaaaa gtcagtcaaa cattgtggtg gctgtaaaga tcagggcgtc
     7381 agatcgctgg cattaagccc ggatgaaaag ttgatttatg cgacttcatt tgaagcgaat
     7441 gcgttatcgg tcattaacgt ggcgacaggg gagattattc agtcgattac caccggtgca
     7501 catcctgaca gccttatcct ctcgcgtgat ggcacaaaag cctgggtgat gaatcgcacc
     7561 agtaatagtg tgtcagcgat tgatctggtg acttatcagc atgtggcgga tatcccgctg
     7621 gagaaatacg acggggcggg gacgagcggt aaaccaggcg cctgggttat ggcactttcc
     7681 ccggatgaaa gaacattact ggttccggga gcaggcagag gtaacatcgt gcggatcaat
     7741 accatcacgc atcagaaaga agactttccc gcaggtgatg cgcgtggaac gatatcggcg
     7801 atgcgttttc gacctgaaaa cggcgaggtt atttttgcag atagtcaggg gatttcacgt
     7861 ataagcgtag gggatcaaca agccagcatt atgacgcaat ggtgtagcag gagcgtttat
     7921 tccgttgagg gtattagccc ggacggtcaa tatttagcgt tggtgtcata tggcctgcaa
     7981 ggttatgtca tcctgctcaa tattaatgcc gggcagatta ttggcgttta tcctgccagc
     8041 tacgtta
//
LOCUS       DAJVOY010000177         7908 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVOY010000177 DAJVOY010000000
VERSION     DAJVOY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.177"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(127..858)
                     /gene="dnaQ"
                     /locus_tag="OQL90_003462"
     CDS             complement(127..858)
                     /gene="dnaQ"
                     /locus_tag="OQL90_003462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1370115.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            923..1390
                     /gene="rnhA"
                     /locus_tag="OQL90_003463"
     CDS             923..1390
                     /gene="rnhA"
                     /locus_tag="OQL90_003463"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1370116.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1387..2109)
                     /locus_tag="OQL90_003464"
     CDS             complement(1387..2109)
                     /locus_tag="OQL90_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1370117.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2143..2898
                     /gene="gloB"
                     /locus_tag="OQL90_003465"
     CDS             2143..2898
                     /gene="gloB"
                     /locus_tag="OQL90_003465"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1370118.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            2970..4328
                     /gene="mltD"
                     /locus_tag="OQL90_003466"
     CDS             2970..4328
                     /gene="mltD"
                     /locus_tag="OQL90_003466"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1370119.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4377..4973)
                     /locus_tag="OQL90_003467"
                     /pseudo
     CDS             complement(4377..4973)
                     /locus_tag="OQL90_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(5004..5804)
                     /locus_tag="OQL90_003468"
     CDS             complement(5004..5804)
                     /locus_tag="OQL90_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1370120.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6045..6959
                     /locus_tag="OQL90_003469"
     CDS             6045..6959
                     /locus_tag="OQL90_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1370121.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(6956..7749)
                     /gene="dkgB"
                     /locus_tag="OQL90_003470"
                     /pseudo
     CDS             complement(6956..7749)
                     /gene="dkgB"
                     /locus_tag="OQL90_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
BASE COUNT         2106 a         1899 c         1920 g         1983 t
ORIGIN      
        1 tgttccgttg ggaacgaggc gaatattacg gattgcctca cccttcgtca acggtttttc
       61 tcatcttttg aatcgtttgc tgcaaaaatc gcccaagccg ctatttttag cgccttttac
      121 aggtatttat gcccgccaga ggcagcttcc gcccttcttc tccaccagat caagacgggc
      181 ttcatgagct gcaagctctt catctgtcgc aaaaacaacg cgtaacttac ttgcctgacg
      241 tacaatgcgc tgaattgttg cttcaccttg ttgctgctgt gtctctcctt ccatcgcaaa
      301 agccatcgac gtttgaccac cggtcatcgc cagataaact tccgcaagga tctgtgcatc
      361 gagtaatgcc ccgtgtagcg ttcgtttact gttatctatt tcgtagcggg cacataacgc
      421 atcgaggctg ttgcgcttac caggaaacat tttcctcgcc accgcaaggc tatcggtgac
      481 cttacagaag gtattggtct tcggaatatc gcgcttaagc aacgaaaact cgtagtccat
      541 aaagccgata tcgaacgctg cgttatggat caccaactcc gcgccgcgga tatagtccat
      601 gaactcatcg gctacttcgg caaacgtggg cttatcgagc aaaaattcat cggcaatacc
      661 atgtacgcca aaggcttccg gatccaccag ccgatcgggt ttgagataaa catggaagtt
      721 attgcccgtc aggcgacggt tcaccacttc aacggcacca atctcaatga tcttgtggcc
      781 ttcatagtgc gcaccaatct ggttcatacc ggtggtttcg gtatcgagaa cgatctggcg
      841 tgtaattgca gtgctcatag cggtcattta tgtcagactt atcgttttac agttcgattc
      901 aattacagga agtctaccag agatgcttaa acaggtagaa attttcaccg atggttcgtg
      961 tctgggcaat ccaggacctg ggggttacgg cgctatttta cgctatcgcg gacgcgagaa
     1021 aacctttagc gcaggctaca cccgcaccac caacaaccgt atggagttga tggccgctat
     1081 tgtcgcgctg gaggcgttaa aagaacattg cgaagtcatt ttgagtaccg acagccagta
     1141 tgtccgccag ggtatcaccc agtggatcca taactggaaa aaacgtggct ggaaaaccgc
     1201 agacaaaaaa ccagtaaaaa atgtcgatct ctggcaacgt cttgatgctg ccctagggca
     1261 acatcaaatc aaatgggaat gggttaaagg ccatgccgga cacccggaaa acgaacgctg
     1321 tgatgaactg gctcgtgctg cggcaatgaa tcccacactg gaagatacgg gctaccaagt
     1381 cgaagtttaa gcttgtggtt tacgacattg ccgggtggct ccaaccgctt ggcgaattcg
     1441 tggcttgttt ttactctgtt tcatcgggtt tagcgttaaa ggaatagtcc gtttccgggc
     1501 aacaataagt tgtaagcaac caagcgcagg aatatgcgca ttcaatagtt ttcctccgtg
     1561 tttgttccac gggagaacgt ggaaacggct ggcgtgcagc acttcaaaat tcaacaaaga
     1621 gagccagtcc agttgccgca tcagagtaaa catccggctg ttatagggcg aggtttttcg
     1681 caataccggc acaagtttgc gtaatcccat taaactaatg ggattgaagc cactaatcac
     1741 cagccagcca tcatcaatca atacccgatc ggtttcacgc aataaacgat gcggatcggt
     1801 gcaccacggc aatgtatgtg ccagtagaca aacatcaacg gatttatcgg caaaaggaag
     1861 atgaagtgga tccgcctgca cctggacggg cattccttgc gcagaaacat tcacttgatg
     1921 agaaaccgcg cacgcttcgc aattgatttc tgcgcttaaa ttgccaatct taagcagatg
     1981 aaaaccatac attttagtga accatgggtt gagctggcgc tccagcgcct tgcgataaag
     2041 ctctccccag ggcaaatcgc cccagcgatc aggagccacg acagtttgag ggactcttgc
     2101 cggtttcatc acaaccttcc gtttcacact aagaggtaat ctatgaatct taacagtatt
     2161 cccgcctttg atgacaatta catctgggtt ttgaatgatg aagcaggtcg ctgcctgatt
     2221 gtcgatcccg gagacgcaga gccagtatta aacgccattg ccgccaataa ctggcaaccg
     2281 gaggccatat ttctcaccca ccatcatcac gatcacgttg gcggcgtaaa agaactggtg
     2341 gaaaagtttc cgcaaattgt ggtgtatggt ccacaagaga cacaagataa gggaacaaca
     2401 caggtagtca aagatggcga aactgccttc gttttggggc atgaatttag tgtaattgcc
     2461 acaccgggtc acactttagg acatatctgt tacttcagta aaccttatct attttgcggc
     2521 gacacgctgt tttctggtgg gtgtggtagg ttgtttgaag ggacagcatc acaaatgtat
     2581 caatcactta ataagttaag tgcgttacct gacgatacat tggtatgttg tgctcatgaa
     2641 tatacgttat caaatatgaa gtttgctttg agtattcttc cgcacgattt gtccataaat
     2701 gattattatc gtaaagttaa ggagttacgg gcaaaaaatc aaataacact acccgtaatt
     2761 ctgaaaaatg agcggcaaat taatgttttt ttaagaacgg aagatattga tttaattaat
     2821 gtaattaatg aagaaacatt attgcaacaa cctgaagagc gttttgcatg gttaaggtca
     2881 aagaaagata ggttctgata aaactttctt gtcatcggct ccgttcgccg ttatgatcgg
     2941 tcgtctttta agcaactatt gacacacaca tgaaggcaaa agcgatatta ctcgcctctg
     3001 tcctgctcgt gggttgccag agtaccggca acgttcaaca gcacgcacag agcctttctg
     3061 cagctggtca aggggaagca gcaaagttta caagtcaggc acgatggatg gacgatggga
     3121 cgtctatcgc gccagatggt gacttgtggg ctttcattgg cgacgagcta aagatgggaa
     3181 ttccggaaaa tgaccggatt cgcgaacaga aacagaaata tttacgcaat aagagctatc
     3241 tccacgatgt aactttacgg gcagagccgt atatgtactg gatagcaggg caagttaaaa
     3301 aacgtaacat gcctatggaa ctggtactac tacccatagt ggagagcgct tttgatcctc
     3361 acgcaacgtc tggcgccaat gccgcaggca tctggcagat cattccgagc acggggcgca
     3421 attatggttt gaaacagacc cgcaattatg acgcgcgtcg cgatgttgtt gcttcaacaa
     3481 ctgccgcgct gaacatgatg cagcgtctga acaagatgtt tgacggcgac tggcttctga
     3541 ccgtagcggc ttataacagc ggcgaaggtc gggtcatgaa ggcaattaaa acgaacaaag
     3601 cgcgtgggaa atccacggac ttctggtcgt taccgttgcc gcaggaaacg aagcagtacg
     3661 tgcctaaaat gctggcattg agtgatattc tcaaaaacag caagcgttat ggcgtacgtc
     3721 tgccaacgac cgatgaaagc cgtgctctgg cgcgtgtgca cctgagcagc ccggttgaaa
     3781 tggcgaaggt tgcagatatg gcggggattt ccgtcagcaa gctgaagaca ttcaacgctg
     3841 gcgtaaaagg ctccacgctg ggcgcaagtg gcccgcagta cgtgatggtg ccaaagaagc
     3901 atgcagatca actgcgtgat tctctcgctt caggcgaaat tgctgctgta cagtcgacgc
     3961 tggttgccga caatacgccg cttaacagcc gtgtttacac cgtacgctct ggcgacacgc
     4021 tttcaagcat cgcttcacgt ctcggcgtaa gcaccaaaga tttgcagcag tggaacaaac
     4081 tgcgcggatc taagctgaag ccaggccaaa gtttgacgat tggtgcaggc agtagcgcac
     4141 agcggttggc aaacaacagc gatagcatta cgtatcgtgt gcgcaaaggc gattcgcttt
     4201 caagcattgc taaacgccac ggcgtgaaca tcaaagatgt gatgcgctgg aacagtgaca
     4261 ctgcgaatct gcaaccaggc gataagctga cgttgtttgt gaaaaacaac aacatgccag
     4321 attcctgaca aaccagataa taaaaaaggc accgattccc ccggtgcctt ttttatttat
     4381 gccgctttat gcgcttctac catgatgata tcactggtga aagagccatc agtctgcaag
     4441 gaaaaatacg ttctcacctc tgtcgatgcg ctctgttggt aaatgcgaat agcgtctact
     4501 aacgcttctg gcgtacgcat tctcacgacc catgaagaaa attccagcgg taacttatct
     4561 gtaattaaat tatcaactat cagattggct tcattgatta acgtcaacca cgcaccgctg
     4621 gcgtagtttc gtacgtgaga ggtatcgcgt aatgcttcta ccgcctgtaa ccagatgtcg
     4681 cgcactgggt gacccggaga cattacatcc atcacaatca gcctaccgcc aggtttcaat
     4741 atcctattca cttctcgcag tgctgcacca acatcatgcc agtgatgggc agaataacgg
     4801 ctgataacaa tatcaaatgc gttatcggca aatggcagac ttgcggcata tccctggcgg
     4861 gtggtgatat ttttcagttg ccgggcttcg gcagcttgtg ccacaacatc cagcatgtgg
     4921 gccgataagt catacgccac caccgcgctc acgttttgcg cagcgccaaa cgttggtctg
     4981 ccctgtggca gacctgacat accttattta tcaggcttgc cgggactgaa ttcaacgagt
     5041 agcggattgt gatcggaagc gcgcgtaacc agtacagaag cttcactgac gttcagacca
     5101 cggtagaaaa caaaatcgag cgggcgacca aacgcccgac ggcgctgatc atcggtaaaa
     5161 cgcacctggc gcagcgacat ttcccgcgca aagcgatata acgcgttcat ccttctacgg
     5221 ctccaggcat tgaaatctcc cgccataatg accgggccgc tgtggtgagc tatctgatca
     5281 ccaataggaa gtaactgctt actatagaca tccacgccca gactgaagtt gacggcgtgt
     5341 atattaacca ccatcaacag gcgggtgtca ggtaatggat agaccgtcac cagtgccgac
     5401 ttcgccagac gcaaaatggg ttctcgttca cgtaacgggc agcaatacac tggatgtgcc
     5461 gccgaaaggg tcattacgcc agaaggatgt tgtggcagca cgaaagcggg tacctgatcg
     5521 gcggcaagat agttagcggt cgcaaactgt actaactctg gcgttgtctg cgcttcctgc
     5581 aataacacca ggtgtgcatc tttgccgtag ttctttaata ccgacaacca ttcagcgcgt
     5641 tgctgtttgt atatgttcca caccaggatc cgaatacgct cttcggtact taacggttcc
     5701 ccaggtggta atgcctggcc gatgctcgca aaagaccccg gcggtaagat cctttccgca
     5761 ggttgtccgg caacatagcg catggcatag gtgttttttc gcacttttga ctttgaaacc
     5821 tctattacgt caacccttcc gcactcggga gggttctact attataggga ttttagctca
     5881 ctctttcaac gagcaattgt atattgttat gtaagcaagt gcttacaccg gtcagaattt
     5941 tgctcattat ggagaacatt aaatcgcatg acatatcacg catcctgatg gtttaatgac
     6001 aaataaattc aaaatatctc ttctattttg caacaggagc aaaaatgaaa gccacgtcgg
     6061 aagaactcgc catttttgtt tcggtcgtcg aaagcggcag ctttagccgg gcagcggaac
     6121 aattagggca agcaaactca gcggtaagcc gggcggtgaa aaagctggag atgaaacttg
     6181 gcgttagcct gcttaatcgg accacgcgac aacttagcct gacggaagaa ggcgagcgtt
     6241 attttcgtcg cgtacagtca attttgcagg agatggcagc ggcagaatca gaaattatgg
     6301 agacgcgtaa tacaccgcgt ggactgttac ggatcgatgc cgcaactcca gtggtgctgc
     6361 actttctgat gccgttaatt aagcctttcc gtgaacgcta tccggaagtc actttgtcac
     6421 tagtctcctc cgaaacgatt attaatttga tcgaaagaaa agtggatgtc gcgatacgcg
     6481 ctggtacgtt aacggattcc agcttacgtg ccaggccgtt atttaacagt tatcgaaaaa
     6541 ttatcgcctc ccccgattat atttcccgct acgggaagcc agaaacgatc gacgatttaa
     6601 agcaacatgt ttgcctggga ttcactgaac ccgcttccct caatacctgg ccgatagcct
     6661 gtagtgatgg acaattacat gaggtgaaat acggtttgtc atccaatagt ggggaaacac
     6721 tgaaacagct ttgcctgagt ggcaacggga ttgcgtgttt gtccgactac atgatcgaca
     6781 gagaaatcgc tcgcggagaa ttggtggagt taatggcaga taaagtgttg ccagtggaaa
     6841 tgccattcag tgccgtctat tacagcgacc gtgcggtaag tacgcgcatc cgggctttta
     6901 tcgatttcct tagcgagcat gtaaaaacag ctcccggagg agctgtcaga gaggcttaat
     6961 cccattcagg agccagacct tccgggctaa ccaggcggtc gttgcaatcc agtgcggcga
     7021 tcgctttttt atcttcggca tcaagctgta aattttgtgc cttaagatta ctttccaggt
     7081 ttttacgttt agtagaagaa ggaattactg agtaaccttc ccccatagcc cacgccagaa
     7141 tcacttgtgc cggagtcgca ttgtgtttag ctgcgatacg agcaataacc tcatctttca
     7201 gggccttacc atacgccagc gtcatatagg aagtaatatg tatgccgtgc tgtttagccc
     7261 aggcaaccac tttacggttt tgcagataag gagagagttc aatctggtta gtagcgatgt
     7321 tttcagcgcc aacagcagca atcgcctttt ccatcaatgg gatcgtgaag ttggaaatac
     7381 cgatctcacg cgtcagccct tgttttttgg cttccagcag cgcctgcata accccttcaa
     7441 cagagacttc atcgtttggt gacggccagt ggattagcgt cagatcaaca taatcggtac
     7501 gcaatttttg caggctctct ttcagacttg ggatcaattt gtctttgctg agattttcaa
     7561 tccagatttt agtggtgatg tagagttcat gacgtggcac gccactttct gcaatcgcct
     7621 gacctactgc ggcttcgtta tcatagattt gtgcggtatc aattgcgcga taaccaagtt
     7681 caagcgccgt tttcacagat gaaataacaa cgtagtcttt caacctaaac caaatgcagg
     7741 gatagccata atatgcctct tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc
     7801 agactgttga ggaaaaaagc gtaaaatgca gaggattttt gcgattctgg caataataga
     7861 tatacagacg aaaaaaaacc tcgagcgtta actcgaggct tcttaata
//
LOCUS       DAJVOY010000178         7866 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVOY010000178 DAJVOY010000000
VERSION     DAJVOY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.178"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(498..860)
                     /locus_tag="OQL90_003471"
     CDS             complement(498..860)
                     /locus_tag="OQL90_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1370122.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            1145..1354
                     /locus_tag="OQL90_003472"
     CDS             1145..1354
                     /locus_tag="OQL90_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370123.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1366..1953)
                     /gene="mtlR"
                     /locus_tag="OQL90_003473"
     CDS             complement(1366..1953)
                     /gene="mtlR"
                     /locus_tag="OQL90_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1370124.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1953..3101)
                     /gene="mtlD"
                     /locus_tag="OQL90_003474"
     CDS             complement(1953..3101)
                     /gene="mtlD"
                     /locus_tag="OQL90_003474"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1370125.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3331..5244)
                     /gene="mtlA"
                     /locus_tag="OQL90_003475"
     CDS             complement(3331..5244)
                     /gene="mtlA"
                     /locus_tag="OQL90_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1370126.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5781..6143
                     /locus_tag="OQL90_003476"
     CDS             5781..6143
                     /locus_tag="OQL90_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1370127.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6146..7282
                     /locus_tag="OQL90_003477"
     CDS             6146..7282
                     /locus_tag="OQL90_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1370128.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1964 a         1909 c         1903 g         2090 t
ORIGIN      
        1 ttgtccattg tattattggt gatttatgtt gctttacatt aatgacagtg atgtgtgtcg
       61 attaaatatt gttttaagca ttcatcataa aaatgtatca ttcgtttcaa aagatgtctg
      121 tgtttgtatg aatatattat tttcgtggat tctaatgatg attattaata aatgtcaaat
      181 atgaaagaaa ccatgtctgg gaaaaaattt acagataatt tattgtatga atatgatgat
      241 taaaatttca caattaaatc cgaaaaaaag agacgtaact gcttgttaat taataaatga
      301 tatcttctat atatttttat tgtttatagg tggctatttc ggtaaaaatg cattttcaga
      361 atatcaacca gcggtaaatc gacgtaaagg gagtgaattg tttggtaatt aggcgagtag
      421 acaggagagt ccccgcattg accagctaag gcaagccaga tgcgggttga tatgatcgaa
      481 ccagtcgtct gaggcgctta aaacgcagtc ttagagaatc cagtcatctc ctgcaggccc
      541 atttcacggc ccagcgcggt cattgggtgc acaaccacca gtccgcgaac acttttcttc
      601 agcttgccca tatcagcctg ctcttttttg gtaattgcgc gctgaaacgg catcttcatc
      661 agcttctgtg cttctttact cagtttttgg ctatgaacct cacgcaggcg agcaatttct
      721 gcttccagcg tggctttctc tttttccagc tcggcatatt tgtctgcggc ttcaaccagc
      781 gacagatcgg cctgctggtg gcggatggcg tccaggcgat cgctgagacg tttgatttcg
      841 tttttttcga cttctttcat gtgatggctt ctgataaaga taaatttata gcccctagta
      901 tgcgcgacca ggtgctttgt tcacaactga tgacattgta atagttctgc aacgtaatca
      961 ttaacatgct caaaaactac gcaattcgtt gattaattaa gaaatctata gaaaaaagat
     1021 aataaacagc agcggatgat tattgaggat tcagcacgtt ttccttaatg tcatctttac
     1081 cgggaaccta aactaaaatt ggtggccgcg cattcccggc gcggggtaat acggagatat
     1141 catcatgggc aaattaggtg aaaacgttcc gcttcttatc gataaagccg tagatttcat
     1201 ggcatcaagc caggcgttcc gggagtatct gaaaaaactt cctccccgta acgcgattcc
     1261 gtccggaata cccgatgaaa gcgtgccgtt atatctacaa cgtctggagt attatcgtcg
     1321 gctttatcgg ccgaagcagg tagaggggca gtaagcagac gttgcttact tttgaaaggc
     1381 tttttttaag ctgattttgg aaatcagctc agtcagtgaa aggaccattg ttgatcgcac
     1441 agcctgttga tagcgctgaa tttgcattgc atacaaactg gagtctgctg gttcaaactg
     1501 tggcggcggt ggtaacgccg ccacgcagtg cagttcacca aagggtccaa ggatttcgtc
     1561 gtcggtaaag gcgtactcgt tgccgtcgtg atttagctct tcacgcaatg ccatcagcag
     1621 ttccgcatct tcgtattcct ggcggttaat gacgcccaac ccgtaaatga gttttaaacg
     1681 cacagaaaga tcgcccagcg gaccgtcgcc gtcgagtaac ggttctacag catacttcac
     1741 cgcgtaatcg tctttgcgga atacctgaag caccagaaga tttaccgcct cggtcaaaag
     1801 ctcgacggcg gtgatcagaa agcttcgcac ggttttgcca gcattcagac gctcaagcac
     1861 acggttttca aaggcctggg tttgttccat tgttgcctgc atatctgaca atctgttatt
     1921 cgggcgcagg gtgtcctgcg cctggtccac cattattgca ttgctttata agcggttacc
     1981 gcctcggata caacctcact gttggcatca agaccggaaa tctgtgccag cgccgcctgc
     2041 ggacctttgt cagcgatcag tgctgccagt tcctgagcct gcggatcatc ttcactgcgg
     2101 aagtgcattg caccagcaat cccctgaatc agattcttgt gcggaaggct gtattccagc
     2161 gtaccgagca gtggcttgat cagacggtcg ccagcactca gtttacgcag cggctgacgg
     2221 cctacgcgct ctacatcatc tttcagatac gggttctcaa aacgaccgag gattttctgg
     2281 atgtacgccg catgtttgtc tgcgtcaaag ccgtagcgct tgatcagtac cgcaccactt
     2341 tcttccatcg caccttttac caccgcgcgg attttctcgt cgagaatagc gtcacgaatg
     2401 gtctgatgac cggccagttt tccgaggtac gcggttatag catgacccgt gttcagggtg
     2461 aagagtttac gttcgacaaa tgccatcagg ttgtcggtta actccatgct tgggatgttc
     2521 ggcagtgcgc ctttgaactg cgttttatcg acaatccatt cgctgaaagt ttctaccgtc
     2581 acttccagcg gatcgttagt tgccgaagcc gaaggcggta cgatgcggtc aacggcggaa
     2641 tcgacaaagc caacgtgttc ttctacccac gctttggcgt cttccggcag ggcgttcatc
     2701 acatggcctt tcagctgcgt ggtaccgcgt accatgtttt cacaggcgat gatgttcagc
     2761 ggggattcat taccttgttc tttgcgtttc accagccctt tggcgatagc cggagcaata
     2821 cgttccagca caaccgggcc aacggcggta gtgactaaat cgacctgagc aatcagatca
     2881 acgacatcat caccaatgct gctgacagca ttgacgccgg aaacggtatc tacctgctcg
     2941 gtttcaccaa ccacatgaac ctgatagcta tgacgggcat tcagggcatc aagtaccacc
     3001 tgattgacat cggcaaacgt cagttggata cccgcgtctg ccagcagttt accgataaag
     3061 ccacgaccga tattacctgc gccaaaatgt aatgctttca tagtattaac cttcatcaat
     3121 gtttttaccc gagagggctg gggtgaggct tttccctcac ccaaaccctc tccgtgagga
     3181 gaggggcagc gccggatgcg gcgtaagcgc cttatccggc ctacaagaac gtgcaaattc
     3241 aacaaatcgt gatgcatcct gtaggcctga taagcgtagc gcatcaggca attaacccca
     3301 ccttctccat gtggagaggg tgggattgga ttacttacga cctgccaaca gttccagcac
     3361 ttcatccacg ctggtggtgt gtgccagacg ctcgatgacg gactcatcat ccagtgcatt
     3421 ggtcaggctg gtgataacct gaatgtgctc gttgttacgg gctgcaatac caatcaccag
     3481 acgggcaatg tcatcttcct cttcaccgaa gcgcacgcct tccgggtact ggcagaacac
     3541 cacgcccgtt ttcagtacgc gatctttcgc ttcaaccgta ccgtgtggca ccgcgataga
     3601 ctcacccaga taagtcgggg tcagtttttc acgatccagc atcgcctgaa cgtattccgg
     3661 ctcaacgtaa ccgcctttca ccagctgctc gccagcaaaa cgaatcgctt cttctttggt
     3721 tgctgctttg cgaccgagga agatgttctc cgcgcccagt ttgaacaggt tagcactgga
     3781 atcgtcaaag ctgtctttca gactgtcttt cactttcact tcgttttcag tatggcgctg
     3841 ggcagcaacc agacgttcgg ttaggctggt gtacaggccg ctgtcgagga agttggtcag
     3901 cgaaatatgc tgtgcctgcg gaacctggcg catagcgcgt tcggtcaggt cacggtgagt
     3961 gatgacgagg tccacatctg gcggcaggtt gttgatcgcg ctgttagtga ccgaaatctg
     4021 cgacagacct gcatcctgaa ttttcttacg cagcacgcct gcgcccatcg cacttgaacc
     4081 cataccggca tcacaggcaa cgatgatttt acgtacgtgg ctcaggtcgt tagtcacatc
     4141 gcccgcagac agcggagatg tgcctttaga ctcagctttc atgtcctgca tacgacgagt
     4201 tgctgcttca atatcatctt cttctttcac tttgctggtt ttcagcaaaa tagcagagac
     4261 aacgaaggag acagccatcg ccgcacacac acccgcgatg ttagcgaaat aagccccttt
     4321 tggtgtcatc gccagtacag caaggataga acccggagat gccggagaaa ccagaccacc
     4381 gcccaggata gtcagcgtga acacgccagt cataccgccg aggatgactg cgaggatcag
     4441 acgcggattc atcagcacat acgggaagta gatttcgtgg ataccaccca ggaagtggat
     4501 gattgccgca ccgcccgcag actgtttagc gctaccacga ccaaagaaca tgtacgccag
     4561 cagcacgccc atacctggac ctgggttagc ttcaatcagg aagaagattg atttacccag
     4621 ttcatgggac tgctgaatac ccagcggcga gaagataccg tggttaatgg cgttgttgag
     4681 gaacaggatt ttcgccggct caacaaagat agacgccagc ggcagcatgt catggacaac
     4741 catgaagtta acgcccgcag ccagcatttt ggacagggct tcaacaatcg ggccaatgcc
     4801 gaggaatgcc agaatagcga ggatcatccc gatgatgcct gcggagaagt tattcaccag
     4861 catctcaaaa ccggatttga tcttaccgtc tacccagcgg tcgaagtgct taatgcacca
     4921 gccgcccagc ggacctgcaa tcatagaacc gaggaacatc ggcatgtctg cgccgacgat
     4981 aacgcccatg gtggtgatgg caccgactac gccgccacgt tcgccgccta ccagcttacc
     5041 accggtataa ccgatcagca gcggcaggag ataagtgatc atcggcccga ccagcttcgc
     5101 cagcgtctcg ttcgttaacc accctgttgg aataaataac gcggtgatga taccccacgc
     5161 gataaacgcg ccgatatttg gcatcaccat gttgctgagg aaacgaccaa agctttgcac
     5221 tttgatctta atatcggatg acataaaaac accccttctt atgtttgctg tcgcgcaggc
     5281 tggaagcccg aggttttttg ttaatgtggc ggcagaggta gccggacctt gtagatactg
     5341 cgaaatctgg cactgaatcg gttaactgtc cagtcgacgg ccttttgtgt gatattaatc
     5401 acataaatat aggaggtaga gaggtaattg acgtgacgtt catcacaaaa cgcttgtgta
     5461 aaaaagcaac aaggcggcga ttttcgctaa aaatggcatt taaatgtgag tagtgtcaca
     5521 tttttgtttt gatggtttgt tgaatctttg tgatctgaat cacaagatat tttcttacgg
     5581 cgttcacctg atgtgatcta cagcatgtta tgccttcgtc atacaatcgt tatgtaacta
     5641 agcaactcat cacctctaaa aattattctt tggtgttatg gcgagaatcg cgaacccgga
     5701 ttattgtcaa aatatcagcc tgactccctt ctggctgcca gcccaaaaac aacactgata
     5761 aacgtgaatc cgaggcagat atgtttctaa actatttcgc gttgggagtg ctgatcttcg
     5821 tctttctggt gattttttat ggaatcatcg cgatacatga catcccttat ctgattgcca
     5881 aaaagcgcaa tcacccccat gccgacgcta ttcatacggc gggctgggtg agcctgttta
     5941 ctctgcatgt tatctggccg tttctgtgga tctgggcgac gctctatcaa ccggagcgtg
     6001 gctggggtat gcagtcacat gttgcgtcgc aggagaaagc gactgacccg gaaatcgcct
     6061 cactttctga ccgaatttcc cggctggagc atcaactcgc cgccgagaaa aagactgact
     6121 attccacgtt cccggagatc taattatgga tctattgatt gttttaactt acgtggcgct
     6181 ggcgtgggcg gtctttaaaa tcttccgcat tccggtaaat cagtggacgc tggcgacggc
     6241 ggcgctggga ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact acaaccaccc
     6301 ttacactttt accgcgcaaa aggcagtgat agcgatccct atcacgccac aggtgacggg
     6361 aattgttact gaagtcactg acaagaataa tcagcttatt caaaagggcg aggtgctttt
     6421 taagctcgac ccggttcgtt accaggcgcg agttgacaga cttcaggctg acctgatgac
     6481 ggcgacgcat aatataaaga cgctgcgtgc gcagctcact gaagcgcagg ccaacaccac
     6541 ccaggtttca gcggagcgcg accgtctgtt taaaaattat caacgttact tgaaaggcag
     6601 ccaggcggcg gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc aaaatttcct
     6661 cgcgcaggat gcgctggtga aaggctcggt ggcggagcag gcgcagatcc agagccagct
     6721 cgacagtatg gttaacggcg agcaatcgca gattgtgagc ttaagagcgc aacttactga
     6781 agcaaaatat aaccttgagc agactgtcat tcgcgcgccg agcaatggct acgttactca
     6841 ggtactgatc cgcccaggta catacgcagc tgccttgccg ctgcgtccgg tgatggtctt
     6901 catccccgag caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc tgttacgtct
     6961 gaaacccggc gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg tgtttcacgg
     7021 caaactgact agtattttac ctgtcgtgcc aggcggttct tatcaggcgc aggggggatt
     7081 gcaatcatta acggtcgtgc ccggcacgga cggtgtgcag ggaaccattg aactggaccc
     7141 taacgatgat atcgatgcct tacccgacgg catctacgcc caggtggcgg tttactccga
     7201 ccatttcagc catgtttcgg tgatgcggaa agtgctgcta agaatgacca gctggatgca
     7261 ttatctttat ttggatcatt gagggggagg ggggagatta tggaactttg ctccttgtaa
     7321 ggactaatta tttttgcata ttacttcatt agctgcgatg atttttcttt aatattcatt
     7381 atgttaataa gttctggatg agtgtgctct cattggattt tttatccgtc ttgttctgcc
     7441 gcaggatgag actatacatc gtcgagatcg caatgatgat atgagttgtt cctcctgcga
     7501 cggctctaat caaaaaagag caggagtgct tttccatcta ttgcgatgat ggctttatta
     7561 tctgaaggat gacagtcaca tttaaaaaat atttaatcta tagatcacaa tgtagggtca
     7621 tggaatatat gtatacaact attttcttct tcacttttaa ttatcgtatt cattataaaa
     7681 ctaagttaaa aaaactcctg gagagtgagt tattaatata tttttaaata aaattaagtt
     7741 atcattcatc gatgcatttt atagaatcat caatggccat aatagtacta ttaactctca
     7801 attttatatt tttatcatca ctacaaatat taactgaatt tatatcaatg atttttttat
     7861 tcttat
//
LOCUS       DAJVOY010000179         7820 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVOY010000179 DAJVOY010000000
VERSION     DAJVOY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.179"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            270..413
                     /locus_tag="OQL90_003478"
     CDS             270..413
                     /locus_tag="OQL90_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1370129.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            426..635
                     /gene="cspE"
                     /locus_tag="OQL90_003479"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             426..635
                     /gene="cspE"
                     /locus_tag="OQL90_003479"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1370130.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            801..1610
                     /gene="rlmA"
                     /locus_tag="OQL90_003480"
     CDS             801..1610
                     /gene="rlmA"
                     /locus_tag="OQL90_003480"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1370131.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OQL90_003481"
     CDS             complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OQL90_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1370132.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2601..3059)
                     /locus_tag="OQL90_003482"
     CDS             complement(2601..3059)
                     /locus_tag="OQL90_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1370133.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3114..3965)
                     /gene="manZ"
                     /locus_tag="OQL90_003483"
     CDS             complement(3114..3965)
                     /gene="manZ"
                     /locus_tag="OQL90_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1370134.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3978..4778)
                     /gene="manY"
                     /locus_tag="OQL90_003484"
     CDS             complement(3978..4778)
                     /gene="manY"
                     /locus_tag="OQL90_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1370135.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4841..5812)
                     /gene="manX"
                     /locus_tag="OQL90_003485"
     CDS             complement(4841..5812)
                     /gene="manX"
                     /locus_tag="OQL90_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1370136.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6275..>7820
                     /gene="yoaE"
                     /locus_tag="OQL90_003486"
     CDS             6275..>7820
                     /gene="yoaE"
                     /locus_tag="OQL90_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1370137.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDVDNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1988 a         1914 c         1940 g         1978 t
ORIGIN      
        1 acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt gaactatgtt
       61 agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca atgatatatc
      121 ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg
      181 tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt gataacagat
      241 ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa gaagtcctga
      301 gtaaacacgt tgatgttgaa taccgcttct ctgccgagcc ttatattggt gcctcatgca
      361 gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt
      421 ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa ggttttggct
      481 tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct atccagggta
      541 atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag gacggccaga
      601 aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc tgaagtgtga
      661 atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact atgctttatc
      721 ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact tgcggccttt
      781 agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag cctctttcgc
      841 gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg aaagaagggt
      901 atgtcaatct gctgcccgtt cagcataaac ggtctcgtga tccgggcgac agcgcggaaa
      961 tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg cgtgatgcaa
     1021 ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg gatattggct
     1081 gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg
     1141 gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg caggtcactt
     1201 tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac gccataatac
     1261 gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc ggcggctggg
     1321 tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg atttacaatg
     1381 aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta cagcagagtg
     1441 cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga
     1501 cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac actggcagca aaagaagtgt
     1561 tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa ccgtggaagt
     1621 gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga atttccgctt
     1681 ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt gacataatca
     1741 aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg aaagcaagac
     1801 caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc agtagccaga
     1861 aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa ccctcaataa
     1921 tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc cattcaagga
     1981 caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg gtttcgacgg
     2041 caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg agggtggcac
     2101 ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga agaacagtag
     2161 cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca tcgcccccag
     2221 caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca ggcatagcga
     2281 ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc cacgttttgc
     2341 aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc ccaaatgagg
     2401 gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt atttcattgc
     2461 tttattgata acgaattcat ttagatttat atatcaatga aacgttaaaa taaataacaa
     2521 ataagggaag aggtaatata gcctaagcta tgtctgatta ttaaaaatac actaaacata
     2581 gctttggcta aattcataac ttattgagtt aaaacgataa gcttataaat tctttccaga
     2641 ttgtcgatat ttcgaacgcg gattaacagc cgacgctgtt ctaattgcat caccaacacg
     2701 ccatcttccg acaagttcat cgctttgatt cggctatatt caatccagac attggcgaag
     2761 aaaaaacctt tttgtttaaa gatgatcttc ggaacgcgga tccagaatat ataaaaaccc
     2821 atcagagcca gtgcgcttaa taaccatgtc gttattaatg caccatgatt cgtgacgttg
     2881 ttatagataa gaatgacaat caatccgacg aagataacgc tatcgatgcg accacgccgg
     2941 agcaaaggaa ttgccagcag ggtggggccg ttacggcggg gcatgatgaa ctgatcgtag
     3001 atcgcgaagg ccaggagtgc ggcgatgaaa agaatcagta ccaggtccgt gattgtcatt
     3061 aatcctccag ataaaaaaac ggggccaaaa ggccccggta gtgtacaaca gtcttacagt
     3121 cccagcaggc cgcaagcgta accagcgata ccgatgacga agaagccaac gatgatccac
     3181 agcgggttaa cttttttgcg cagtagccac atacaagcaa aggtcagcag cagtggtacc
     3241 aggcctggca ttaactggtc cagaatagtc tggacagtag taacgtgttc tttgcccgtc
     3301 tggtcagtaa tgcgagagac aaccagcggg atgttgacat gtgtccactt gttaaccaat
     3361 gcccccatga caaacaggcc gaggatagac gccccttccg tcagtttttg caggaagcca
     3421 ccacccatat ctttaacgat atcgatacct ttggagtaac catacgctac gccgtagtaa
     3481 cgggttgcca gacgcaccag gttaaacagg atgaagaaca gcagcggacc taacaggctg
     3541 ccgctcatcg cgataccggc acccagtgct gcaaataccg gacgtacggt tccccagaag
     3601 atcgggtcgc ctacaccagc cagcggcccc atcaaaccga ctttgatacc gttgatagca
     3661 ccgtcgtcga tctctgcgcc attagcacgc tgttcttcca gcgccagggt tacgccgaga
     3721 atcggcgcag ccacgaacgg ctgggtgtta aagaactcca ggtgacggcg aatagcttgt
     3781 ttacgagctt cgttgttctc agggtagagg cgacgaattg ccggtaccat agagaagcag
     3841 aaacccagtg cctgcatacg ttcgaagttc catgaaccct ggaagaggtt agaacgcagg
     3901 aagacgccac gaatatcact ttgagtgagt tttttctcgg tggtagtttg agttgtatca
     3961 accatttcgc tcacctgtta gtccagttcg ttatcgagat cgttgttacc agcagcctga
     4021 gcaggcgcac cggctacgcg gttgtatttc gggctaagtt ggatgtagag cactgccata
     4081 acagtaccga tcacacccag agcaaccagg ttaaagttgg tgaatgctgc ggttacgaag
     4141 ccgaggtaga agaacggcat caggtagcca gcacgcatca tgttgataac catcgcataa
     4201 ccaaccacca cgatcatgcc accggcgata ttcagaccat tggtcaccac ttccggaatc
     4261 gcattcagca tgttctgtac ttcgctggta ccaacagaca gcgcaacgat gacggccgga
     4321 atagccacac gcattgcttg caggaacaga gaagaaacgt ggatccagga aatcgctgtc
     4381 aggttgccgt tatcagcagc cttatccgca gcgtgctgga aagcaacggt aatagtacga
     4441 acgatgatgg tcagtacctg gcccgcagcg gccagaggga ttgccagtgc gatacctgca
     4501 ccaatgctct gatgacctgc gataaccaga atggtagaaa tgatagaagc cagagcggcg
     4561 tcaggcgcaa ctgcagcacc gatgttcatc cagcccagcg cgatcatttc cagcgtacca
     4621 ccgataataa taccggtttt catatcccca agaacgatac ccaccagggt acacgcgatt
     4681 agcggacggt gaaactgaaa ttcatcgagg attgatccca tacctgcgat acaggctacg
     4741 ataaatacca gcacaatttg aagagtggta atctccattg tacttctcct gtttacgtaa
     4801 gacttaagtg tgaaaactga gtgataatca acacaatacg ttacttatcg attttgctga
     4861 tcagatccat cattttcagt ttcggatcgg tggaaacctt acggacttcc agctcaatac
     4921 cgcgcgcatt cagtttcttg aacgcctcga tatctttttc atcaaccgaa accgcgttat
     4981 tcacctgggt tttaccctga cggaatgcca taccaccgac gttaacagag gtgattttca
     5041 cgccgccttc aacgagacgc tctacatctg ttgggttggt aaataacagc attacgcgtt
     5101 cgccagcata tttcgggttg ttgtagacgc gaatcatttt ggcaacatca actacgtgtg
     5161 ctgttacgcc cggaggtgca acctgggtga gcagtgtctt acgaacggta tccgcagcca
     5221 cttcatcact aacaacaata atacgggaga cattggtttc tttggtccag cgggtggcga
     5281 cctgaccgtg aatcagacgg tcgtcgatac gcgcaaggcc aataaccatg tagtcgtttg
     5341 gccccattgg ttttgccgga gttggagccg cttttggtgc tgcggcagcg ggtgctggcg
     5401 cggctttttc aaccggtttg gctttcagtg ctttcacacc ttcacggcct gtttctactg
     5461 ccagcgcaac cagttcatca aagcttgggt catcatcacg agccattaac gtttccacga
     5521 gcattggaat gttaacgcct gcaatgactt cataatgctc tttgtcgacg acaatgcggc
     5581 tggcagcatt gaacgggctg cctccccatg tatcaacgag aaacagcacg cctttagtgg
     5641 tgtcgagttt tgccaactga gcgttgtact tttcaatcag cgtttcggca ttttcacctg
     5701 gaacgaaatc gatccagccg acgttttcct gctcgcctaa cagcatttct gccgttttaa
     5761 gcaactgctc tgcagcccaa ccatgtgtgc ctataacaat agcaatggtc acttgctacc
     5821 tcctttatta tcgttaacac ctcaacgtgc cagatgtatt tttgaatcgt cgtccacaat
     5881 cgaatcgatt cagataaggg ctacaaccaa aaacccctgc gtttcgcgat ttattttaga
     5941 tatcgaaaaa attattttat gtgatgaaga tccgtaattt aactttcgat tagcagaaat
     6001 ttcgaaaggt aaaatatcct tgtcacattc gtttgcaaag gaaggtaaat ctttgccaaa
     6061 tcagaggcgt ctctgatatg tttaactccc gtttaacaac catggagtat agggcagtag
     6121 cccgcagtat ggatcgtcac cgacgtcatt tcagcatcag gccttttaac gcctgccttt
     6181 ctggcactct atgccgcacc tttcgtttgc attttgtcgt tacgcctgca ttatttctgg
     6241 cgtcgaatag ctattcctta agcaggagct tgtcatggaa ttcttaatgg acccctcaat
     6301 ttgggcgggg ctactcacgc ttgttgttct cgaaattgtg ctgggtatcg ataacctggt
     6361 cttcatcgcc attcttgctg acaaactgcc gccaaaacaa cgcgataaag cgcgtttgct
     6421 ggggttatca ctggcgctga ttatgcgtct ggggctgctg tcgctgattt catggatggt
     6481 cacgctgacc aaaccgctat ttaccgtcat ggatttctcc ttctccggac gcgacctgat
     6541 tatgttgttc ggggggatat tcttgctgtt caaagcaaca accgaactgc atgaacggct
     6601 ggaaaaccgc gatcatgatt ccggccacgg taaaggctac gccagtttct gggtggtcgt
     6661 cacacagatt gtcatccttg acgccgtctt ctcgttggat gcggtaatta ctgcagtagg
     6721 gatggttaac catctgccgg tgatgatggc ggcggtagtg attgcgatgg cggttatgtt
     6781 gctggcatcc aaaccgctga cgcgattcgt taaccagcac cccacggtgg tggtgctctg
     6841 tctgagcttc ctgttaatga ttggtcttag tctggtggca gaaggtttcg gtttccacat
     6901 tccgaaaggt tacctgtatg ccgcgattgg cttctcgatc atcatcgaag tgtttaacca
     6961 gattgcgcgt cgcaacttta ttcgccacca gtcgactttg ccgctgcgag cgcgtactgc
     7021 cgatgccatc ctgcgtttga tgggcgggaa acgtcaggcc aatgttcagc acgatgtcga
     7081 taacccgatg ccgatcccgg aaggtgcatt tgccgaagaa gaacgttaca tgattaacgg
     7141 cgtactgacg ctggcgtcgc gttctctgcg cgggatcatg acgccgcgcg gtgaaataag
     7201 ctgggttgac gctaatctcg gggtcgatga aatccgcgag caactgctct cttcaccgca
     7261 cagtctgttc ccggtatgtc gcggtgaact ggatgaaatc atcggtattg tacgtgctaa
     7321 agaactgctg gtggcgctgg aagagggcgt tgatgtggcg gcgattgctt cggcgtctcc
     7381 ggcgattatc gtcccggaaa ccctcgatcc gatcaacctg ttgggcgtgc tgcgtcgtgc
     7441 tcgcgggagc tttgttatcg tgaccaacga gtttggtgtg gtacaaggtc tggtcacgcc
     7501 gctggatgtg ctggaagcca ttgcgggtga attcccggac gctgacgaaa cgccggaaat
     7561 cattactgat ggtgacggct ggctggtaaa aggcggtaca gatttgcatg ccttgcagca
     7621 ggcgcttgat gttgagcacc ttgccgatga cgatgatatc gcgacggtcg cgggcctcgt
     7681 gatctcggca aatggtcaca ttccccgtgt gggcgatgtg attgatgtag ggccactgca
     7741 tatcaccatc attgaagcca atgattatcg tgttgatctg gttcgcattg ttaaagagca
     7801 accggcgcac gatgaagatg
//
LOCUS       DAJVOY010000180         7817 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVOY010000180 DAJVOY010000000
VERSION     DAJVOY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.180"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            301..636
                     /locus_tag="OQL90_003487"
     CDS             301..636
                     /locus_tag="OQL90_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1370138.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            796..1239
                     /gene="yjdN"
                     /locus_tag="OQL90_003488"
     CDS             796..1239
                     /gene="yjdN"
                     /locus_tag="OQL90_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCS1370139.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1372..2160
                     /gene="phnC"
                     /locus_tag="OQL90_003489"
     CDS             1372..2160
                     /gene="phnC"
                     /locus_tag="OQL90_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1370140.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2185..3201
                     /gene="phnD"
                     /locus_tag="OQL90_003490"
                     /pseudo
     CDS             2185..3201
                     /gene="phnD"
                     /locus_tag="OQL90_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
     gene            3327..4106
                     /gene="phnE"
                     /locus_tag="OQL90_003491"
     CDS             3327..4106
                     /gene="phnE"
                     /locus_tag="OQL90_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCS1370141.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4127..4852
                     /gene="phnF"
                     /locus_tag="OQL90_003492"
     CDS             4127..4852
                     /gene="phnF"
                     /locus_tag="OQL90_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCS1370142.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4853..5305
                     /gene="phnG"
                     /locus_tag="OQL90_003493"
     CDS             4853..5305
                     /gene="phnG"
                     /locus_tag="OQL90_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCS1370143.1"
                     /translation="MHADIATRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5302..5886
                     /gene="phnH"
                     /locus_tag="OQL90_003494"
     CDS             5302..5886
                     /gene="phnH"
                     /locus_tag="OQL90_003494"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCS1370144.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5886..6944
                     /gene="phnI"
                     /locus_tag="OQL90_003495"
     CDS             5886..6944
                     /gene="phnI"
                     /locus_tag="OQL90_003495"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCS1370145.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            6937..7782
                     /gene="phnJ"
                     /locus_tag="OQL90_003496"
     CDS             6937..7782
                     /gene="phnJ"
                     /locus_tag="OQL90_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCS1370146.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
BASE COUNT         1790 a         2335 c         2121 g         1571 t
ORIGIN      
        1 tatttagtgt gttgtttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca
       61 gaaagataaa aatatcatgt gaataaaaaa agaacaggta gagcattaac attatcttaa
      121 ataataaata gaggcaaaaa gattattttc tttttgcgtt tcctttcaaa tgaaaacgat
      181 cgtcgtctaa aatcagcagt acccccgaca aactcaggga ttttgtgtat aattgcggcc
      241 tttttcggca atctgccgtt ttttggcgct tttgccctgc tgacttttga ggaaatccac
      301 atgtcattac cacactgccc aaaatgcaac tccgaataca cttacgaaga taacggcatg
      361 tacatctgcc cggaatgtgc ctacgaatgg aacgacacag aacctgcaca ggaaagcgac
      421 gagctgatcg ttaaagatgc taacggcaat ctgctggctg acggcgacag cgttaccatc
      481 attaaagatc tgaaggtgaa aggtagctct tcgatgctga aaattggcac caaagtgaaa
      541 aacatccgcc tggttgaagg cgaccataac atcgattgca aaatcgacgg ttttggtccg
      601 atgaaactga aatctgagtt tgtgaaaaag aactgattgt attgtgatcg gtaagccgga
      661 taaggcgctc gcgccacatc cggcaacgat gccgactgcc tgatgcgacg ctgaccgcgc
      721 cttatcaggc ttccctctca tctgtataaa tttcgaacta cacttaactg gcttctctta
      781 actgaggtca ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac
      841 gcgattgcct attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc
      901 gaaatgccaa aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc
      961 gataccgcca tcgcccacgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat
     1021 gctataccgt caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag
     1081 gtcgaagaag gaaaacgctg gtttgataac cttgccgcta acggaaaaat tgaaatggcc
     1141 tggcaggaaa ctttctgggc gcatggcttc ggcaaagtca ccgataaatt tggcgtaccg
     1201 tggatgatta atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc
     1261 ccgcactgtc atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa
     1321 tcgtcacaat aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg
     1381 attatccgtg tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt
     1441 gatctgaaca ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa
     1501 tccacacttt tacgtcactt aagcggtttg attaccggcg ataaatccgc cggcagccat
     1561 atcgagctgc tgggccgcac agtccagcgc gaaggccgtc tggcgcgcga tatccgcaaa
     1621 agccgcgccc ataccggcta catcttccaa caattcaacc tggtgaatcg cctgagcgta
     1681 ctggagaacg tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc
     1741 tggttcaccc gcgagcagaa acaacgcgcg ttacaggcgc tgacccgcgt tggcatggtg
     1801 cattttgccc atcaacgcgt ttccaccctc tccggcggac agcagcagcg tgtggcgatt
     1861 gcccgcgcgc tgatgcagca ggcgaaggtg attctggccg atgaacccat cgcctcgctg
     1921 gacccggaat ccgcccgcat cgtgatggac accctgcgcg acatcaatca gaacgacggc
     1981 atcaccgtag tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc
     2041 gtcgccctgc gccaggggca cgttttctac gacggcagca gccaacagtt tgataacgaa
     2101 cgttttgacc atctctaccg cagcattaat cgcatcgaag agaacgcgaa agctgcctga
     2161 catccccatc attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc
     2221 agcatgttca gcctcagcac cctgttaagc ccggcacacg ccgaagagca ggaaaaggcg
     2281 ctgaatttcg gcattatttc aacggaatca cagcaaaacc tgaaaccgca atagacgcca
     2341 ttcttacagg atatggagaa gaagctgggc gtgaaggtga acgccttctt tgccccagac
     2401 tacgcaggca ttatccaggg aatgcgcttc aataaagtgg atatcgcctg gtacggcaac
     2461 ctgtcggcaa tggaagcggt ggatcgcgcc aacggccagg tcttcgccca gacggtcgcg
     2521 gcggatggat cgccaggtta ctggagcgtg ttgatcgtca acaaagatag tccgatcaac
     2581 aacctgaacg atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac
     2641 tccacctctg gcttcctcgt ccccggttac tacgtctttg ccaaaaacaa tatctccgcc
     2701 agcgacttta agcgcaccgt caacgctggg catgaaacca acgcgctggc cgtcgccaac
     2761 aagcaggtgg atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg
     2821 ccagagaagc tgaaagaact gaaggtgatc tggaagtcgc cgctgatccc aggcgatccg
     2881 atcgtctggc gcaagaatct ctccgaaacc accaaagaca agatctacga cttctttatg
     2941 aactacggaa aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggccccgttc
     3001 cgcgcctcca gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagatg
     3061 cagggcgtaa aaagcaataa aggactgaat gagcaggaca agctggcgaa aaccaccgag
     3121 attcaggcgc agctggatga cttggaccgc ctgaacaacg cgttaagcgc gatgagttcg
     3181 gtgagtaaag cggtgcagta atgtgctcga tcggtcccct ctcccttgag ggagagggtt
     3241 agggtgaggg ggaaatgcgc acgctgatgc cctcatcccg gccctctccc acagggagag
     3301 ggagaaaaca ttaaacggag ccaaacatgc aaaccatcac catcgcccca cccaagcgta
     3361 gctggttctc gcttctgagc tgggccgttg ttctcgccgt gctggtcgtc tcgtggcagg
     3421 gcgcggaaat ggccccgctt acgctgatta aagacggcgg caacatggca accttcgttg
     3481 ccgacttctt cccgcccgat ttcagccagt ggcaggatta cctcaccgaa atggccgtca
     3541 cgctgcaaat cgccgtctgg ggcaccgcgc tggcagtggt tctctccatc cccttcggtc
     3601 tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca gcccgttcgc cgcctgatgg
     3661 atgcctgccg cgccattaac gaaatggtct tcgccatgct gttcgtggtc gccgtcggtc
     3721 tcggcccgtt cgctggcgtg ctggcgctat ttatccacac caccggcgtg ctctccaagc
     3781 tgctttccga agcggtggaa gcgatcgaac ccggcccggt agaaggcatt cgcgccaccg
     3841 gcgccaacaa gctggaagag atcctttacg gcgtgctgcc acaggtaatg ccgctgctga
     3901 tctcctactc cctctatcgc ttcgaatcca acgtccgctc ggcgaccgtc gtcggcatgg
     3961 tcggtgcagg cgggatcggc gtcaccctgt gggaagcgat tcgcggtttt cagttccaac
     4021 aaacctgcgc cctgatggtg cttatcatcg tcacggtcag cctgctggat ttcctctctc
     4081 aacggttgcg taagcacttt atctgataag cgaggcattg atatctatgc acttgtctac
     4141 acatccgacc agctacccaa cacgctatca ggagatagcc gcaaaacttg agcaggagct
     4201 tcgtcaacac tatcgctgcg gcgactatct tcccgccgag cagcagctgg cggcgcgctt
     4261 tgaggtaaat cgccacaccc tgcgccgcgc catcgaccaa ctggtggaaa aaggctgggt
     4321 acagcgccgt cagggcgtcg gcgtgctggt gctgatgcgc ccgttcgatt acccgctcaa
     4381 cgcccaggcg cgttttagcc agaacctgct ggatcagggc agccatccca ccagcgaaaa
     4441 actgctgtcg gtgctgcgcc cggcatctgg tcacgtcgct gacgcgctgg ggataaccga
     4501 gggggagaac gtcatccacc tgcgcaccct gcgccgggtc aacggcatcg cgctctgttt
     4561 aatcgaccac tactttgcgg acctcaccct ttggccgacg ctgcaacgct tcgacagcgg
     4621 ctcgctgcac gattttctgc gcgagcaaac cgggattgcg ctgcgccgca gccagacgcg
     4681 gatcagcgcc cgccgcgccc aggccaaaga gtgccagcgt cttgaaatcc cgaatatgtc
     4741 gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt gaaagcagcc cggcggagta
     4801 ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact atggagcact gaatgcacgc
     4861 agatatcgcg acccgccagc actggatgtc catgctggcg cacagccaac cggctgaact
     4921 ggcagcccgc ctgaacgcgc tgaacatcac cgccaactat gaagtgatcc gcaccgccga
     4981 aaccggcctg gtacagattc agccgcgaat gggcggtacc ggcgaacgct tttttgccgg
     5041 cgacgccacg ctgacccgcg ccgccgtacg cctgactgac ggccccctcg gctacagctg
     5101 ggtgctgggg cgcgacaaac agcacgccga acgctgcgcg ctgattgacg cgttgatgca
     5161 gcaatctcgc cattttcaaa acttatcaga aacccttatt gccccactgg acgctgaccg
     5221 tatggcacgc attgccgcac gccaggccga agtgaacgcc agccgggtcg acttctttac
     5281 gatggttcgc ggagacaacg catgaccctg gaaaccgctt ttatgcttcc tgtgcaggat
     5341 gcccagcaca gttttcgtcg cctgttaaag gccatgagcg agccgggcgt gattgtcgcc
     5401 ctgcatcagc tcaaacgcgg ctggcagccg ctgaatatcg ccaccaccag cgtgctgctg
     5461 acgctggctg ataacgacac gccggtgtgg ctttccgcgc cattaagtaa cgatatcgtc
     5521 agccagagcc tgcgttttca taccaacgcg ccgctggtca atcagtcgga actggcgacc
     5581 ttcgcggtaa cggatgaggc gatttccagc gaacagctca acgctctttc cagcggcacc
     5641 gctgttgcgc cggaagcggg cgcgacgctg attttacagg tcgtcagcct gagcggcggg
     5701 cgcatgctgc gccttactgg cgcgggtatt gccgaagaac gaatgatcgc tccgcagctg
     5761 ccggagtgca ttctgcacga actcaccgag cgcccgcacc cgttcccgct cggcatcgac
     5821 ctgatcctga cctgcggcga gcggctgctg gctatcccgc gaaccactca tgtggaggtg
     5881 tgctgatgta cgttgccgtg aaagggggcg agaaggagat cgacgccgcc cacgccctgc
     5941 aagagagccg acgccggggc aataccgatt tgcctgaact gagcgtcgcc cagattgaac
     6001 agcagcttaa tcttgcagta gatcgcgtga tgaccgaagg cggcattgcc gaccgcgaac
     6061 tggcggcgct ggcgctgaaa caggccagcg gcgataacgt cgaagcgatt ttcctgctac
     6121 gcgcctaccg caccacgctg gcgaagctgg cggtaagcga gccactcgac accacggaga
     6181 tgcgtctcga acggcgtatt tcagcggttt ataaagatat tcccggcggt cagttgcttg
     6241 gcccaaccta cgactacacc catcgcctgc tcgatttcac cctgctggca aacggcgaag
     6301 cgccgacgct gaccaccgca gacagcgaac aatccccgtc gccgcacgtt ttcagcctgc
     6361 tggcgcgtca ggggctggcg aagtttgaag aggatagcgg cgcacagccg gatgacatca
     6421 cccgcactcc gccagtttac ccctgttcac gttcttcccg tttgcagcag ttgatgcgcg
     6481 gcgacgaagg ctatttgctg gcgctggcct actctaccca gcgcggttac ggacgcaatc
     6541 acccgttcgc gggcgaaatc cgcagtggtt acatcgacgt gtcgattatg ccggaagagc
     6601 tgggatttgc ggtgaacgtc ggcgaactgc tgatgaccga gtgtgaaatg gtcaacggtt
     6661 ttattgaccc gccggatgag ccgccgcact tcacgcgcgg ctacgggctg gtgttcggca
     6721 tgagcgagcg caaagcgatg gcgctggttg accgtgcgtt gcaggctccg gagtacggcg
     6781 agcacgcgac aggcccggcg caggatgaag agttcgtgct ggcacatgcc gacaacgtcg
     6841 aagccgcagg ctttgtctcg cacctcaaac tcccccacta cgtcgatttc caggccgaac
     6901 tggagctact caaacgtctg caacaggagc agaaccatgg ctaatctgag cggctacaac
     6961 tttgcctacc tcgacgagca gaccaaacgc atgatccgcc gcgccatctt aaaagcggtg
     7021 gcgatccccg gttatcaggt gccgtttggc gggcgcgaga tgccgatgcc ctacggctgg
     7081 ggaaccggcg gcattcagct caccgccagc gtgattggcg aaagcgacgt gctgaaggtg
     7141 attgaccagg gcgcagacga caccaccaac gccgtgtcga ttcgcaactt ctttaagcgc
     7201 gtgaccgggg taaacaccac tgaacgtacg gacgatgcga cgcttatcca gacgcgccat
     7261 cgcatccccg aaacgccgct gaccgaagat cagatcatta tcttccaggt gccgatcccc
     7321 gaaccgctgc gctttatcga gccgcgcgaa acggaaaccc gcaccatgca cgcgctggaa
     7381 gagtacggcg tgatgcaggt gaaactgtat gaagatatcg cccgcttcgg tcatatcgcc
     7441 actacctacg cctatccggt gaaggtgaac gggcgctacg tgatggaccc gtcgccgatc
     7501 ccgaaattcg ataacccaaa aatggacatg atgcccgccc tgcaactgtt cggcgcgggg
     7561 cgcgagaagc gcatctatgc ggtgccgccg tttacccgcg tggaaagtct cgatttcgac
     7621 gatcacccgt tcaccgttca gcagtgggat gagccatgcg ccatctgcgg atcgacccac
     7681 agctatcttg atgaagtggt gctggatgac gccggaaacc gcatgtttgt ctgctccgat
     7741 accgattatt gccgccaaca gagcgaggca aaaaaccaat gaatcaaccg ttactttcgg
     7801 tcaataacct gacccac
//
LOCUS       DAJVOY010000181         7750 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVOY010000181 DAJVOY010000000
VERSION     DAJVOY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.181"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..592
                     /locus_tag="OQL90_003497"
     CDS             <1..592
                     /locus_tag="OQL90_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCS1370147.1"
                     /translation="PGDNKDATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIH
                     FQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKA
                     QFEKLSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQ
                     LISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSER"
     gene            955..1992
                     /gene="dusA"
                     /locus_tag="OQL90_003498"
     CDS             955..1992
                     /gene="dusA"
                     /locus_tag="OQL90_003498"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1370148.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2126..2368
                     /gene="pspG"
                     /locus_tag="OQL90_003499"
     CDS             2126..2368
                     /gene="pspG"
                     /locus_tag="OQL90_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1370149.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2533..3516)
                     /locus_tag="OQL90_003500"
     CDS             complement(2533..3516)
                     /locus_tag="OQL90_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1370150.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3599..5014
                     /gene="dnaB"
                     /locus_tag="OQL90_003501"
     CDS             3599..5014
                     /gene="dnaB"
                     /locus_tag="OQL90_003501"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1370151.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5067..6146
                     /gene="alr"
                     /locus_tag="OQL90_003502"
     CDS             5067..6146
                     /gene="alr"
                     /locus_tag="OQL90_003502"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1370152.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6399..7592
                     /gene="tyrB"
                     /locus_tag="OQL90_003503"
     CDS             6399..7592
                     /gene="tyrB"
                     /locus_tag="OQL90_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1370153.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1878 a         1854 c         2026 g         1992 t
ORIGIN      
        1 tcctggggat aataaagatg cgactcccat tgaacaatgt acttttgatg tagaaactaa
       61 gaatggcctt ccctccacgg ttcagctatt ttatgaggaa tctaaggata atgaaccttt
      121 agcgaatata cattttcaag caataggttc tggtttttta acgtttgtta atgcctgcca
      181 ggaacatgat gacaacagct taaaattatt tgcttcgctg ttaatttcac tttcatattc
      241 tagtgcctac gcagatttat cagaaacagt gtatattaat gaaaataatg agagctacct
      301 gaaagctcag tttgaaaaat tatctcaacg tgatatgaag aagtacctgg gagagatgaa
      361 gcgtctggct gatgggggag aaatgaattt tgatggctat ctggataaga tgtcacatct
      421 ggtgaatgaa gggacgctcg atcctgatat tttaagcaaa atgcgagatg ctgcaccaca
      481 attaattagc ttcgcgaagt cgtttgaccc aacctcaaag gaagagatta aaatacttac
      541 agacacttct aaattaattt atgatttgtt cggggttaaa tcggagagat aatatgtgaa
      601 gttcttcgat agtatggaag gcattatata aaaggaccca atatttattg ggtccttttt
      661 tctctatcaa tgctattagc agggagatat atcaccaaag tttaatgtgt gattttttat
      721 ttatcgtcga acctggattg tttatcattg gccttaacaa agttaacggc taataagatt
      781 atttccatca cttcgtgaga gcttcatgcc ttgagaggat ctcaattttc ttttgcaatg
      841 agacaggcgc ttcctgttgt tatggtatag taccccgcta ttgagcctcc tgaatagtga
      901 tgctgaataa cataacccca tgatatatcg ataaaataat ctctacattt gaaaatgcac
      961 ggtaattctg aaatgcaaaa aatcaaccaa accagcgcaa tgcctgaaaa aactgacgtt
     1021 cactggagtg gtcggtttag cgttgcacca atgctcgact ggacggacag acattgccgc
     1081 tatttcttgc gtctgctttc ccgcaatacg ttgctgtata ccgaaatggt gaccacaggg
     1141 gcgattattc acggtaaagg tgattacctg gcgtacagtg aagaagaaca tccggtagcg
     1201 ttgcaactcg gcggtagcga tccggcggcg ctggcacagt gtgcgaagct ggcagaagcg
     1261 cgtggatatg atgagatcaa cctgaatgtc ggctgcccgt ctgaccgggt gcagaacggc
     1321 atgtttggtg cgtgtctgat gggtaatgcg cagctggttg ccgattgcgt gaaagcgatg
     1381 cgcgatgtgg tgtcgattcc ggtgacggtg aaaacgcgta ttggcatcga tgaccaggac
     1441 agctatgaat ttctctgcga tttcatcaac accgtttccg gcaaaggcga gtgtgggatg
     1501 ttcatcatcc atgcacgtaa agcctggctt tcggggttaa gcccgaaaga aaaccgtgaa
     1561 atcccgccgc tcgattatcc gcgtgtgtat caactgaagc gtgactttcc gcatctgaca
     1621 atgtcgatta acggtggtat caagtcgctg gaagaggcca aagcacacct gcaacatatg
     1681 gatggcgtga tggtcgggcg cgaggcgtat cagaatccgg gtattctggc ggcggtagac
     1741 cgggagatct ttggttcctc ggataccgat gcctatccgg tggcggtagt gcgcgccatg
     1801 tatccgtaca ttgagcgtga actcagccag gggacgtatc tcggccatat tacccggcat
     1861 atgttgggct tgttccaggg tattcctggc gcgcggcagt ggcggcgtta tttaagtgaa
     1921 aatgcccata aagcgggtgc agacattaat gtgctggaac acgcgctcaa actggtggcg
     1981 gataagcgtt aacttttcac caaaaagtag tcaaattcac cacgccctgc gcaccgtcgc
     2041 ggggcgtttt gctgttaaat caatagatta tttttggcat gattcttgta atgccagcaa
     2101 gagatttcat atttgggaga gcatcatgct ggaactactt tttgtgattg gcttttttgt
     2161 catgctgatg gtcaccggcg tttcgttgct gggcattatc gccgcgctgg ttgtggcgac
     2221 ggccattatg ttcctcggcg gtatgctggc attgatgatt aagttgctgc cgtggttact
     2281 actggcgatt gcggtggtgt gggttattaa ggcgattaaa gcaccaaaag tgcctaaata
     2341 tcagcgttat gaccgctggc gttactaagg gattgtgcgg atgatcacaa cctaaggttt
     2401 tatccttaga acaaaatagg aattgataat caaatctgtc actattgcgc ctctaacaga
     2461 ttcatcgtgc tgtaccctac atacagccga ctataaaaag aaagggcttc ccaggtggaa
     2521 gccctatttc ttttatggaa tcagcaggct ggaaccttgc gtcgcccggc tttccagaat
     2581 ctcatgcgca cgctgcgcat ccttcagcgg atatttctgc tgctcggcga catcgacctt
     2641 aatcacaccg ctggcaatca aagagaacag ttcattactg gcctcggtta attcctcccg
     2701 tgtggtgata tagccttgca gggaagggcg tgtcacatac aacgagcctt tttgattgag
     2761 aatgcctaag ttcacaccgg taaccgcacc tgatgagttg ccaaaactga ccattaagcc
     2821 gcggcgttgc aggcaatcca gcgaccgttc ccaggtgtct ctgcccacgg aatcgtacac
     2881 tacgcgcact ttcttaccgc cggtgatctc tttcaatcgc tcgaccagat tctcttcacg
     2941 atagttaata acctgccacg cgccggcttt tagcgcgctc tgcgcttttt gcgcggttcc
     3001 tacggtgccg ataagtttcg cgcccagagc ttttgcccac tggcaggcaa tcaagccaac
     3061 gccgccagcc gctgcgtgga acaggaactg ttcatcgggt ttaatttcat aggttttgcg
     3121 cagcagataa taaaccgtta agcctttcag gaaagatgct gcagcttgct caaaagaaat
     3181 agccgcaggc agaatcgccg ctttatccgc attaatgtta tgcacagagc tgtaagcgcc
     3241 taacgccgac tgcgcataga ctacacgatc gcctgcctta atatgcttta caccactgcc
     3301 gactttactc acgatgcctg ccgcttcggt acctgatccg ctgggtagtg atggcggcgg
     3361 gtaaaggccg ctgcggatat acgtgtcgat aaaattgatg ccgatggctt tattttcgac
     3421 ctggatttca ttctctgccg gatcggcagg agtgaactct acggcttgaa gtacttccgg
     3481 gccaccgtgc ttgtgaaatt caattcgtgt tgccatgtgt cctccagaac gtatcgtcag
     3541 ggtctgcttc atatggtaaa gtttcgaccc attctttatc tcggtaactc cattcactat
     3601 ggcaggaaat aaacccttca acaaacagca ggctgaaccc cgcgaacgcg atccacaagt
     3661 tgccgggctg aaagtgcctc cgcactcgat cgaagcggag cagtcggtgt tgggcggttt
     3721 aatgctggat aacgaacgct gggacgatgt agccgagcgt gtggtggcag acgattttta
     3781 cacccgccca caccgccata tctttactga aatggcgcgt ttgcaggaaa gcggtagtcc
     3841 tatcgatctg attacccttg cggaatcgct ggaacgccag gggcaactcg atagcgtcgg
     3901 tggttttgct tatctggcag agctgtcaaa aaatacgcca agtgcggcga acatcagtgc
     3961 ttatgctgac atcgtgcgtg aacgtgccgt tgtccgtgag atgatctcgg ttgcgaatga
     4021 gattgctgaa gccggttttg atccgcaggg gcgtaccagc gaagatctgc tggaccttgc
     4081 tgaatcccgc gtctttaaaa ttgccgaaag tcgtgcaaac aaagacgaag ggccgaagaa
     4141 catcgccgat gtgctcgacg caaccgtggc gcgtattgag cagttgtttc agcagccaca
     4201 cgatggcgtt accggagtaa acaccggtta tgacgatctc aacaaaaaaa ccgctggctt
     4261 gcagccgtcg gatttgatca tcgtcgccgc gcgtccgtcg atgggtaaaa caacatttgc
     4321 gatgaacctc gtcgaaaacg cggcgatgtt gcaggataaa ccagtactta tcttctcgct
     4381 ggagatgcct tcagaacaga ttatgatgcg ttctctggcg tcgctgtcgc gcgttgacca
     4441 gactaaaatc cgtaccgggc agctcgatga tgaagactgg gcgcgcattt ccggcaccat
     4501 gggtattttg ctcgaaaaac gcaatatcta tatcgatgac tcctccggcc tgacgccaac
     4561 ggaagtgcgt tcccgcgcac gacgtattgc ccgtgaacac ggcggcatcg ggcttatcat
     4621 gatcgactac ctgcaactga tgcgcgtacc ggcgctttcc gataaccgta cgctggaaat
     4681 tgcagaaatc tctcgctcgc tgaaagcact ggcgaaagaa ctgaacgtgc cggtggtggc
     4741 gctgtcccag ttgaaccgtt ctctggaaca acgtgccgac aaacgcccgg tcaactccga
     4801 cctgcgtgaa tctggctcta tcgagcagga tgcggacttg atcatgttta tctatcgtga
     4861 tgaggtgtat cacgaaaaca gtgatttaaa aggcatcgcg gaaattatta tcggtaaaca
     4921 acgtaacggc ccaatcggga cggtacgcct gacctttaac ggtcaatggt cgcgcttcga
     4981 caactatgcg gggccgcagt acgacgacga ataataatta ttttatgaat taggcaatta
     5041 aagcaaacac ttatcaagga acacaaatgc aagcggcaac tgttgtgatt aaccgccgcg
     5101 ctctgcgaca caacctgcaa cgtcttcgtg aactggcccc tgccagtaaa atggttgcgg
     5161 tggtgaaagc gaacgcttat ggtcacggtc ttcttgagac cgcgcgaacg ctccccgatg
     5221 ctgacgcctt tggcgtagcc cgtctcgaag aagctctgcg actgcgtgcg gggggaatca
     5281 ccaaacctgt actgttactc gaaggctttt ttgatgccag agatctgccg acgatttctg
     5341 cgcaacattt tcataccgcc gtgcataacg aagaacagct ggctgcgctg gaagaggcta
     5401 gcctggacga gccggttacc gtctggatga aactcgatac cggtatgcac cgtctgggcg
     5461 taaggccgga acaggctggg gcgttttatc atcgcctgac acagtgcaaa aacgttcgtc
     5521 agccggtgaa tatcgtcagc cattttgcgc gcgcggatga accaaaatgt ggcgcaaccg
     5581 agaaacaact cgctatcttt aatacctttt gcgaaggcaa acctggtcaa cgttccattg
     5641 ccgcgtcggg tggcattctg ctgtggccac aatcgcattt tgactgggtg cgcccgggca
     5701 tcattcttta tggcgtctcg ccgctggaag atcgctccac cggtgccgat tttggctgtc
     5761 agccagtgat gtcactaacc tccagcctga ttgccgtgcg tgagcataaa gccggagagc
     5821 ctgttggtta tggtggaacc tgggtaagcg aacgtgatac ccgtcttggc gtagtcgcga
     5881 tgggctatgg cgatggttat ccgcgcgccg ctccatccgg tacgccagtg ctggtgaacg
     5941 gtcgcgaagt accgattgtc gggcgcgtgg cgatggatat gatctgcgta gacttaggtc
     6001 cacaggcgca ggacaaagcc ggggatccgg tcattttatg gggcgaaggt ttgcccgtag
     6061 aacgtatcgc tgaaatgaca aaagtgagcg cttacgaact tattacgcgc ctgacttcaa
     6121 gggtcgcgat gaaatacgtg gattaatcgt tctgtaatat ttgattgtct gtgccggatg
     6181 cggcgtgaat gccttatccg gccaataaaa tcctaaaaat tcaataagtt gatgttcttt
     6241 catgctctta taaaggtcgt gcctctggcg gatgttcgtt tgtcatgagt ctcactctgt
     6301 tgctaattgc cgttcgctcc tgaacatcca ctcgatcttc gccttcttcc ggtttattgt
     6361 gttttaacca cctgcccgta aacctggaga accatcgcgt gtttcaaaaa gttgacgcct
     6421 acgctggcga cccgattctt acgcttatgg agcgttttaa agaagaccct cgcagcgaca
     6481 aagtgaattt aagtatcggt ctgtactaca acgaagacgg aattattcca caactgaaag
     6541 ccgtggcgga ggcggaagcg cgcctgaatg cgcagcccca tggcgcttcg ctttatttac
     6601 cgatggaagg gcttaacagc tatcgccatg ccattgcgcc gctgctgttt ggtgcggacc
     6661 atccggtact gcaacaacag cgcgtagcaa ccattcaaac ccttggcggc tcaggggcat
     6721 tgaaagtggg cgcggatttc ctgaaacgct acttcccgga atcaggcgtc tgggtcagcg
     6781 atcctacctg ggaaaaccac gtagcaatat tcgccggggc tggattcgaa gtgagtactt
     6841 acccctggta tgacgaagcg actaacagcg tgcgctttaa tgacctgttg gcgacgctga
     6901 aaacattacc tgcccgcagt attgtgttgc tgcatccatg ttgccacaat ccaacaggtg
     6961 ccgatctcac taatgatcag tgggatgcgg tgattgaaat tctcaaagcc cgcgagctta
     7021 tcccattcct cgatattgcc tatcaaggat ttagtgccgg tatggaagag gatgcctacg
     7081 ctattcgcgc cattgccagc gctggattac ccgctctggt gagcaattcg ttctcgaaaa
     7141 ttttctccct ttacggcgag cgcgtcggcg gactttctgt tctgtgtgaa gatgccgaag
     7201 ccgctggccg cgtactgggg caattgaaag caacagttcg ccgcaactac tccagcccgc
     7261 cgaatttggg tgcgcaggtg gtggctgcag tgctgaatga cgaggcattg aaagccagct
     7321 ggctggcgga agtagaagag atgcgtactc gcattctggc aatgcgtcag gaattggtga
     7381 aggtattaag cacagagatg ccagaacgca atttcgatta tctgcttaat cagcgcggca
     7441 tgttcagtta taccggttta agtgccgctc aggttgaccg actacgtgaa gaatttggtg
     7501 tctatctcat cgccagcggt cgcatgtgtg tcgccgggtt aaatacggca aatgtacaac
     7561 gtgtggcaaa ggcgtttgct gcggtgatgt aatgcaggaa agcaggctgg agttacccag
     7621 cctgcagtga aattaaactg tcgtcgcttt cactatttct ttgtagatga tttttttgat
     7681 gccgttgttc tacgtgagag ataataaaac gttgtcagtt cttttattgt taagtttatc
     7741 ccaattaact
//
LOCUS       DAJVOY010000182         7650 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVOY010000182 DAJVOY010000000
VERSION     DAJVOY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.182"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            380..988
                     /locus_tag="OQL90_003504"
     CDS             380..988
                     /locus_tag="OQL90_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1370154.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1086..2477
                     /gene="selA"
                     /locus_tag="OQL90_003505"
     CDS             1086..2477
                     /gene="selA"
                     /locus_tag="OQL90_003505"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1370155.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2474..4318
                     /gene="selB"
                     /locus_tag="OQL90_003506"
     CDS             2474..4318
                     /gene="selB"
                     /locus_tag="OQL90_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1370156.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4508..5659
                     /gene="yiaY"
                     /locus_tag="OQL90_003507"
     CDS             4508..5659
                     /gene="yiaY"
                     /locus_tag="OQL90_003507"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCS1370157.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            5824..7362
                     /gene="aldB"
                     /locus_tag="OQL90_003508"
     CDS             5824..7362
                     /gene="aldB"
                     /locus_tag="OQL90_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCS1370158.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT         1808 a         1917 c         2136 g         1789 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgctgccgg ttttccgctc atttctcatc cttgttttat cgggttattt
      181 gtctgctgac aggcaaaacc ctacagattt ataagaaagc ctgaagtgag atatccatca
      241 gagtgaaata attaggaaaa tatttatatc accatgataa atatgtgatt tatttggaag
      301 taacaaacca tcaacatttt ctgccctttt tcccatcacc aggccatact gatatttttc
      361 ctcgcacaaa ggactcggca tgaaactcgt cggtagctac accagcccgt ttgtacgcaa
      421 actttctatt ctgttgttag aaaagggcat aactttcgaa tttattaatg aactgcccta
      481 taacgcggac aacggcgtgg cgcaatttaa cccgttagga aaagtgccgg tgctggtgac
      541 cgaagagggc gaatgctggt ttgattcgcc gatcatcgct gaatatattg agttaatgaa
      601 tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg ttgcgggtgc gcaaaattga
      661 ggcgctggcg gatggcatta tggatgccgg gctggtatcg gtgcgtgaac aggcgcgtcc
      721 ggcggcgcag cagtctgaag atgaattgtt acgccagcgg gagaaaatca accgcagtct
      781 ggatgtgctg gaaggatatc tggtcgatgg cacactcaaa accgatacgg tcaatctggc
      841 gactatcgcc attgcctgtg ctgtcggata tctcaatttc cgccgcgttg cgccaggttg
      901 gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac ctgtttagcc gcgaaagttt
      961 tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt cctcctgacc catctcacgt
     1021 tacaatccgt ggttatgtta aacgcccttc tccatgtgag agggccttga ttagccaggt
     1081 ttcctatgac aaccgaaacg cgttccctct atagtcaact tccggctatt gatcgcttat
     1141 tgcacgatag ctccttcctt tctttgcgtg atacttatgg tcacacccgc gtggtggaat
     1201 tgttgcgtca gatgctcgac gaagcgcgag aagtgattcg tgacagccag acgcttcctg
     1261 cgtggtgtga aaactgggcg caagaagtcg atgcccggtt gacgaaagag gcgcagagcg
     1321 cgctgcgtcc ggtgatcaac ctgacgggaa ccgtgctgca taccaacctt ggacgagctt
     1381 tacaggcgga agccgcggtg gaagccgttg cgcaggctat gcgttcgcca gtgaccctcg
     1441 agtacgatct ggacgacgct ggacgcggac atcgcgatcg ggcgctggcg caactgctgt
     1501 gccgtattac gggggcggaa gatgcctgta tcgtcaataa caatgcggcg gcggtgttat
     1561 tgatgttggc ggccactgcc agcgggaaag aggtggtagt atctcgcggc gaattggtgg
     1621 agattggcgg cgcgtttcgt attcccgatg ttatgcgtca ggcaggctgc accctacacg
     1681 aagtagggac caccaaccgc acgcacgcga atgattatcg tcaggcggtg aatgaaaata
     1741 ccgcactgtt gatgaaagta cataccagta actacagcat tcaggggttc accaaagcga
     1801 tagatgaagc ggaactggtg gcgctcggca aagagctgga tgttcccgta gtgactgatt
     1861 taggcagtgg ctcgctggtc gatcttagcc agtacggttt gccgaaagag ccaatgccgc
     1921 aggagttgat tgcggcgggc gtcagtctgg tgagcttctc cggcgacaag ttgttaggcg
     1981 ggccgcaggc agggattatc gttggtaaaa aagagatgat cgcccgcctg caaagccacc
     2041 cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc ggcgctggaa gccacgttgc
     2101 gtctttattt acaccctgaa gctctgagtg aaaaattacc gaccctgcgc ctgcttaccc
     2161 gcagcgcaga ggtcattcaa atccaggcac aacgtttaca ggctcccctt gccgcacatt
     2221 acggcgcgga gtttgcggta caggttatgc catgtctttc gcagattggc agtggttcgc
     2281 tgccggttga tcgcctgccg agcgcggcat taacgtttac accccatgat ggacgcggta
     2341 gccaccttga gtcattagcc gcccgctggc gtgaattgcc agtgccggtg attggtcgta
     2401 tttatgacgg acgattgtgg ctggatttac gctgccttga agatgagcaa cggtttttgg
     2461 agatgttgtt gaaatgatta ttgcgactgc cggacacgtt gaccacggca aaacaacctt
     2521 attgcaggcg attactggcg taaatgctga ccgtctgccg gaagaaaaaa agcgcggcat
     2581 gaccatcgat ctcggctatg cctactggcc gcagccggat ggtcgcgtgc ctagttttat
     2641 cgacgtaccc ggtcatgaaa agtttctttc caacatgctg gcgggcgttg gtggtatcga
     2701 tcacgcgctg ttggtggtgg cgtgcgatga cggcgtgatg gcgcagaccc gtgagcatct
     2761 ggcgattttg cagctgaccg gtaacccgat gctgacagtg gcgctgacca aggccgatcg
     2821 cgtggacgaa gcgcgtgttg atgaggttga acgccaggta aaggaggtgc tgcgggaata
     2881 cggttttgct gaggcaaaac tgtttatcac cgcagcaacc gaaggtcggg gaattgatgc
     2941 cctgcgcgag catctgcttc agttaccagg acgtgaacac gccagccaac atagtttccg
     3001 cctcgcgatc gaccgcgcat ttaccgtaaa aggtgccggg ctggtcgtca ccggtacggc
     3061 gttaagcggg gaagtgaagg taggcgattc actctggctg actggtgtaa ataaaccgat
     3121 gcgtgtacgt gcgctgcatg cacaaaacca gccaacagaa accgcccatg ccgggcagcg
     3181 tatcgcgctt aacatcgcgg gtgatgcgga aaaagagcag attaaccgtg gcgactggct
     3241 gcttgccgat gcgccgccag agccgttcac acgggtgatt gtcgagcttc aaacccatac
     3301 accgctgacc cagtggcagc cgctgcatat tcaccacgcc gccagccacg tcacgggacg
     3361 cgtttcactg ctggaagata accttgctga actggtcttc gacaccccgt tatggctggc
     3421 agataacgac cgcctggtat tgcgcgatat ctctgcccgc aacacgctgg ccggagcgcg
     3481 cgtcgtgatg cttaacccgc cgcgtcgcgg taaacgtaaa ccagagtatc tgcaatggct
     3541 ggcttccctc gccagggcac agaatgatag cgaagcgtta gccgttcacc tgcaacgcgg
     3601 cgcggttaat cttcctgatt tcgcctgggc gcgccagctc aacggcgaag ggatgcgcga
     3661 actgctgcaa cagcctggct atattcaggc tggttatagc ttgttgaatg cgccggttgc
     3721 cgctcgctgg caacggaaaa ttctcgacac attagcgact tatcatgagc aacatcgcga
     3781 tgaacctggg cctgggcgcg aacgtctgcg acgtatggcg ttgccaatgg aagatgaagc
     3841 gctagtactg ttgctgattg aaaagatgcg cgaaagcggc gacatcctca gtcatcacgg
     3901 ttggctgcat ctgccggatc acaaagcggg cttcagcgaa gagcagcagg ccatctggca
     3961 aaaagcagag ccgctgttta gtgacgagcc gtggtgggtg cgtgatctgg caaaagagac
     4021 gggtaccgac gagcaggcaa tgcgcttgac gctaagccag gcggcgcagc aaggaataat
     4081 taccgcgatc gtcaaagatc gttattaccg taacgatcgg attgtcgaat ttgccaatat
     4141 gatccgcgat ctcgatcagg agtgtggttc aacctgcgcg gcggatttcc gcgatcgctt
     4201 aggcgtaggc cgaaagctgg caattcagat tctggaatat tttgaccgca ttggctttac
     4261 gcgtcgtcgt ggaaatgatc atttattgcg cgacgcatta ttatttccgg aaaaataagg
     4321 aaatgattaa ttaattttta aaataaatta atacaaattt cttatgattt taaaaaaagc
     4381 acattgttta atgaatacaa tgtgcttttt attagattaa ttttgataat taataataat
     4441 gatcatgttt tcgaatgaaa atcctcagta agcttcccac ccttttttac actttcagga
     4501 gtgtgttatg gcatcttcaa ctttctttat tccttctgtg aatgtcatcg gcgctgattc
     4561 attgactgat gcaatgaata tgatggcaga ttatggattt acccgtacct taattgtcac
     4621 tgacagtatg ttaacgaaat taggtatggc gggcgatgtg caaaaagcac tggaagaacg
     4681 caatattttt agcgttattt atgatggcac ccaacctaac cccaccacgg aaaacgtcgc
     4741 cgcaggtttg aaattactta aagagaataa ttgcgatagc gtgatctcct taggcggtgg
     4801 ttctccacac gactgcgcaa aaggtattgc gctggtggca gccaatggcg gcgatattcg
     4861 cgattacgaa ggcgttgacc gctctgcaaa accgcagctg ccgatgatcg ccatcaacac
     4921 cactgcgggt acggcctctg aaatgacccg tttctgcatc atcactgacg aagcgcgtca
     4981 tatcaaaatg gcgattgttg ataaacatgt cactccgctg ctttctgtca atgactcctc
     5041 tctgatgatt ggtatgccga agtcactgac cgccgcaacg ggtatggatg ccttaacgca
     5101 cgctatcgaa gcatatgttt ctattgccgc cacgccgatc actgacgctt gtgcactgaa
     5161 agccgtgacc atgattgccg aaaacctgcc gttagccgtt gaagatggca gtaatgcgaa
     5221 agcgcgtgaa gcaatggctt atgcccagtt ccttgccggt atggcgttca ataatgcttc
     5281 tctgggttat gttcatgcga tggcgtacca gctgggcggt ttctacaacc tgccacacgg
     5341 tgtatgtaac gccgttctgc tgccccatgt tcaggtattc aacagcaaag tcgccgctgc
     5401 acgtctgcgt gactgtgccg ctgcaatggg cgtgaacgtg acaggtaaaa acgacgcgga
     5461 aggtgctgaa gcctgcatta acgccatccg tgaactggcg aagaaagtgg atatcccggc
     5521 aggcctacgc gacctgaacg tgaaagaaga agatttcgcg gttctggcga ctaatgccct
     5581 gaaagatgcc tgtggtttta ctaacccgat ccaggcaact cacgaagaaa ttgtggcgat
     5641 ttatcgcgca gcgatgtaat catcatttcc acaacggctg gcaaattgtc agccgctttt
     5701 tcaactatct ctgtaaccct tgcccgtaaa ttcgtgatag ccgtcgtaaa gctgttaccg
     5761 actggcgaag atttcgccag tcacgtctac ccttgttata cctcacaccg caaggagacg
     5821 atcatgacca ataatccccc ttcagcacag attaagcccg gcgagtatgg tttccccctc
     5881 aagttaaaag cccgctatga caactttatt ggcggcgaat gggtagcccc tgccgatggt
     5941 gagtattacc agaacctgac gccggtgact gggcagctgc tgtgcgaagt ggcgtcttcg
     6001 ggcaaacgag acatcgatct ggcgctagat gctgcgcaca aagtgaaaga taaatgggcg
     6061 cacacctcgg tgcaggatcg cgcggcgatt ctgtttaaga ttgccgatcg aatggaacaa
     6121 aacctcgagc tgttagcgac agctgaaacc tgggataacg gcaaacccat tcgcgaaacc
     6181 agtgctgccg acgtgccgct ggcgattgat cattttcgct atttcgcctc gtgtattcgg
     6241 gcgcaggaag gtgggatcag tgaagttgat agcgaaaccg tggcctatca tttccatgaa
     6301 ccgttaggcg tggtggggca gattatcccg tggaacttcc cgctgctgat ggcgagctgg
     6361 aaaatggctc ccgcgctggc ggcgggcaac tgtgtggtgc tgaaacccgc acgtcttacc
     6421 ccgctttctg tactgctgct aatggaaatt gtcggtgatt tactgccgcc gggcgtggtg
     6481 aacgtggtca atggcgcagg tggggaaatt ggcgaatatc tggcgacctc gaaacgcatc
     6541 gccaaagtag cgtttaccgg ctcaacggaa gtgggccaac aaattatgca atacgcaacg
     6601 caaaacatta ttccggtgac gctggagttg ggcggtaagt cgccaaatat cttctttgct
     6661 gatgtgatgg atgaagaaga tgcctttttc gataaagcgc tggaaggctt tgcactgttt
     6721 gcctttaacc agggcgaagt ttgcacctgt ccgagtcgtg ctttagtgca ggaatctatc
     6781 tacgaacgct ttatggaacg cgccatccgc cgtgtcgaaa gcattcgtag cggtaacccg
     6841 ctcgacagcg tgacgcaaat gggcgcgcag gtttctcacg ggcaactgga aaccatcctc
     6901 aactacattg atatcggtaa aaaagagggc gctgacgtgc tcacaggcgg gcggcgcaag
     6961 ctgctggaag gtgaactgaa agacggctac tacctcgaac cgacgattct gtttggtcag
     7021 aacaatatgc gggtgttcca ggaggagatt tttggcccgg tgctggcggt gaccaccttc
     7081 aaaacgatgg aagaagcgct ggagctggcg aacgatacgc aatatggcct gggcgcgggc
     7141 gtctggagcc gcaacggtaa tctggcctat aagatggggc gcggcataca ggctgggcgc
     7201 gtgtggacca actgttatca cgcttacccg gcacatgcgg cgtttggtgg ctacaaacaa
     7261 tcaggtatcg gtcgcgaaac ccacaagatg atgctggagc attaccagca aaccaagtgc
     7321 ctgctggtga gctactcgga taaaccgttg gggctgttct gatataagaa gctggtcgca
     7381 ttgggtattc attgcctgat gtgacgctta cgcgtcttat catgcctacg ggaacctgac
     7441 cgtaggccgg ataaggcgta cacgccgcat ccggcataaa caaagcgcac gttgttacaa
     7501 tttacgtttt aaacctacgt gcgcaataat ccagactacg ccaacagaag tgcgtttctg
     7561 gggcgtgttt tgtcaggagt ttaatctcgc gtttcgatgg tgagcgggcg aactaggccg
     7621 taaatcacca ttggtgatcc gaggccaatc
//
LOCUS       DAJVOY010000183         7604 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVOY010000183 DAJVOY010000000
VERSION     DAJVOY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.183"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..291)
                     /locus_tag="OQL90_003509"
     CDS             complement(<1..291)
                     /locus_tag="OQL90_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCS1370159.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKE"
     gene            complement(285..1523)
                     /gene="preT"
                     /locus_tag="OQL90_003510"
     CDS             complement(285..1523)
                     /gene="preT"
                     /locus_tag="OQL90_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCS1370160.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(1920..2159)
                     /locus_tag="OQL90_003511"
     CDS             complement(1920..2159)
                     /locus_tag="OQL90_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1370161.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2162..2881)
                     /gene="sanA"
                     /locus_tag="OQL90_003512"
     CDS             complement(2162..2881)
                     /gene="sanA"
                     /locus_tag="OQL90_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1370162.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(3031..3915)
                     /gene="cdd"
                     /locus_tag="OQL90_003513"
     CDS             complement(3031..3915)
                     /gene="cdd"
                     /locus_tag="OQL90_003513"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1370163.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPYAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(4045..4740)
                     /locus_tag="OQL90_003514"
     CDS             complement(4045..4740)
                     /locus_tag="OQL90_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1370164.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYGSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(4737..5135)
                     /locus_tag="OQL90_003515"
     CDS             complement(4737..5135)
                     /locus_tag="OQL90_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1370165.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5374..6324
                     /gene="dusC"
                     /locus_tag="OQL90_003516"
     CDS             5374..6324
                     /gene="dusC"
                     /locus_tag="OQL90_003516"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS1370166.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(6711..6794)
                     /gene="yohP"
                     /locus_tag="OQL90_003517"
     CDS             complement(6711..6794)
                     /gene="yohP"
                     /locus_tag="OQL90_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1370167.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            7018..>7604
                     /locus_tag="OQL90_003518"
     CDS             7018..>7604
                     /locus_tag="OQL90_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCS1370168.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEW"
BASE COUNT         1962 a         1955 c         1905 g         1782 t
ORIGIN      
        1 ttctttcagc cgacgcaggg cggccagatt ctcctccagc ggatgctcgg caatctgctc
       61 catatttttg aagccgataa aaccggtatc ttctttcacc agatgatcaa aacgcggcga
      121 gacttcgttg gcgataaaaa agccgatcgt tttaaacacc acaccgcccc aacctgtgtc
      181 gtaggctttg gcacacatct catagcagtt gcctaccggc gaagaagaga ggcagaacgg
      241 gttgggaaac ttcacgccgc aaaaagtaat cgaaagatct ttcgttaaca tgagcaagct
      301 ccctctaaat agtgatgaat cgcctcggcg gcttctttcc cggttttcac ggcatagacc
      361 atggttttgt caccctcaac aatatcgcca gcagcaaaga cttgcgggtc gcgggtctgg
      421 taattttgtg ttttgatggt gttacgctgc ggctctaact ccgcaaaggc atctagtctg
      481 gcatgctgac cgacggcgag aataatttta tctgccgcca tcgtcagttc gcccgataac
      541 cgtacatgct taaacgtgac tttattgcct tccacggcta ctggcgtgaa tccatcgatg
      601 atcgaaacgc ccagctcccg ggcgctggta aactcttttt cgcttgccgg aaactcatct
      661 aactcttcac gcgctacgca agttaccgcc tgacagccga gaactttcag cgtgctggct
      721 acgtccatcg tgacatcacc gccgccgata attaatgcgc tttgtggaat gctgatatcg
      781 ccttgcgcct gtcgtgcacg ttgcaagaag tcgacggcaa tctcaacgtc actatgctca
      841 aacagcggta gcccggaacc gcttgataac cccacggtga ccagtaccgc gcggttttct
      901 gctttcagct gctcaagggt gagtgtgtta ccgacttcgt tgttgcactt aatggtcacg
      961 cccatttttt caatacgggc gatctctgca tccagtactg attgcggtaa ccggaattgc
     1021 ggaatgccgt tacgcaacca gccaccgggt tgtgcttctt tctcatagat cgtgacgtca
     1081 taaccctggt ttgtcagtgt cacactggcc tgcaatccgg caggacctgc gccaataatc
     1141 gcgactttgc cgagcgtttt agtaccgggc tgataaattt ccatcccggt ttgttgttca
     1201 aaatcagtaa caaaacgctg taagcggccg atatcaatgg gagcatcaac accggcacgg
     1261 gtacaaccgc tttggcataa tttctccgtc gggcacactc tggcacaaac ggcaccgagg
     1321 gcattatttt cgcgaattgt ctcggcagcg cctttaaaat tacgaaagta gattgagcga
     1381 ataaatttcc ccggatcggt ctgtgccggg caagcctgac tacagggagc gtcgtgacat
     1441 aataaacagc gagaggcttc tttaatcgcc agtaacggcg taaaagccgg agtgagttca
     1501 tccagataat tttgttgcgg catgtgatct tccttaatgc tatgagtaat taaggcgaac
     1561 gctaaatata ctcgtcatac ttcaagttgc atgtgctgcg tctgcgggtt attcggccca
     1621 tccatgggcc tcacccctta tggggccgct gcaggcagcg ttcaaatccg ctccatgcgg
     1681 atttgtcgct caccccagtc acttacttat gtaagctcct ggggactcac tcacttgtcg
     1741 ccttcctgca actcgaatta tttagagtat aaatattgcg tgctgatatt aaggctatgc
     1801 gcgtggggaa atttatactg tgaaaggatt cacaaatagc tgcggttcag aatgttatat
     1861 ccgcagctta ttgagtgaag taataattat tctcaataat taacagaaaa gagaaaatat
     1921 taacgataga aaataagata aacaatcatg ccgatagata aaattccgaa gccgatatat
     1981 gccaccatat aagcgaaaaa gagtccggga ttttttgctc gccagacata caccggttcc
     2041 ggtgcatttt taacccgttt atcaatcgcg cctgcgcgcc agcctttcca tgcttcgcgg
     2101 aaacaaaata tcggaataag gaaaaaaacg gcaacgacaa aaaactgctg tacatccata
     2161 actactttcc ttgtttcttt tgtaattcaa gtaactgttc gggtgtgacg gcgggatacc
     2221 cctgcgcatc ttccggcacc tggtgcatag ccggaatagg gaccagcggc cctaaaaaac
     2281 gcggttcacg tttaaaaata taaaggtcag ccagcgcacc gaaacgggcg gcaaattcgc
     2341 gaatacgtac tgacagcata tctttcggtg acggtacggc ataacactga gcctgaatcc
     2401 ccatatgcag cgcaataaat aatgcccgct cacagtggaa acgttgggtg ataataatga
     2461 aatcattagt atcgaaaact ttgcgtgtac gcacgatgga atccagcgta cgaaagcctg
     2521 cgtaatcgag aacaatatct gatgggtcga caccagcagc gattaaatct ttgcgcatgg
     2581 tcatcggctc attataactt tgcaatgcgt tatcgccgct cagtaataga taatttacct
     2641 taccgctgtt ataggcatta atcgctcctt gaatgcggta gcgataatac tgattaatta
     2701 cgccagtacg ataatatttt gctgttccga gcaccacacc gacctggcgg taggggagat
     2761 cctgcaattc gtcgtagata taaggcgcgg ttttccagct catccagcga tcgaggccga
     2821 gcacagtcaa caacagcaag ccgatcagga ctaacaggct gaggaacacg cgctttaaca
     2881 tgcaggagac tcgatctaag gaaaatttcc atcaggctac tttacccgct ggttttgcgc
     2941 aagaaacggc aaactgtttg ccgggaattt cgacaccagg agagggctgg caagccggac
     3001 aaggcgttca cgccgcatcc ggcaccaggc ttaagcgaga agcactcggt cgatactgtg
     3061 acagccgaga gctttcagcg ttgcggaggt ggcatcccac tgaatcaacg gcgcatcggc
     3121 tttttctgcc agaaccgcgc gctggatatc cgggtaatca taacccttga gattcaacag
     3181 aattaacgct ccctgcaacg gtggcagagt cgggttgaat gcggcgtttt cagcgtagct
     3241 gccactgaaa atacgaccgt ctttacattc cagcgcgaca ccgcttggcg acttactgta
     3301 aggcatgtgc gaacggtttg ccgctgcaat cgccgcctga gaaagcgcat caccagtcag
     3361 cgcatagccg tgatcctgct cgtccatcag cagcgtttta atctccagat ctttcggccc
     3421 aaaggcatac ggcagatagt cacgcagcgc gtgtgcctcg cggcccggca gatgaatacg
     3481 cagatccaga ccgctgttca gttcattcat aaactgacgg cagtgaccac aaggcgtgta
     3541 gttaacggtg atggctgcaa gcgctttttc accactcaac caggcgtggc tgatcgcgct
     3601 ttgttcggca tgaacggttt gctgcattgt cgcaccaata aattccatat tggcaccgaa
     3661 ataccaggtt ccgctcacac cgcgcgcaat tgcgccaaca ttaaaattcg acaatggcgt
     3721 acgcgcacag gccgctgcca gcggaagtag tgcgaatgcc agcgcgtctt cgtccagccc
     3781 cgttgcgctc ttcagcgatg agacttgctc cccggtcaac aaagcgggga agtacttgtc
     3841 tgccagaata ggttccagtg cagattgcaa gttatccgca agttgggcaa aagcggtttg
     3901 aaaacgtgga tgcatggcgt tgcctcataa tcatgtaatg ggataacagt ttacgaaccc
     3961 gttatggctt tatatgtgat ctgaatctca ttaattatgc aacgcgtgaa acttacatca
     4021 aaaatgcgcg acgcatcgca aattttagcc cattactgcc agaataatcg ggaaaaggaa
     4081 cggtgcgatc agcgaagtaa ttatcccgca taacaccagc gctagcgaac taaatgcacc
     4141 ttcctgataa tccagctcgg cgcagcgcgc cgtaccgagg gcgtgcgagg cagtccccat
     4201 tgccagaccg cgcgcagctt tggtacgaat acgcatcgca ttaagcaatg tatggccaaa
     4261 taccgcgccg aggatgccga cgaaaatcac gcaaactgcg ctgattgccg gaataccgcc
     4321 aatactgccg ccaaccgcca ttgcaatagg cgtggtgact gatttcggca ggattgacgc
     4381 ggcgatttcc ggtgaagcgc ccatcaacaa tgccacggaa gtccccgtca ccatcgcaac
     4441 cacgctgccg ataaaacaga tggtgatgat cgatttccag cgcgcgcgga tctggtgtag
     4501 ctgctcatat aaaggatagg ccagcgcgac gaccgccggt tgcagcagat cgtttagcac
     4561 ttcgctacct ttaaagtagc tgccgtaaga gatgccggtc agcattaaaa aagggatgat
     4621 caccaccatt gctaccagca acgggttaag caagggaaac ttataccgtg ccgccagttt
     4681 gcgggcggca aaaaagacga tcaatgtcag tggtaatgac caccagatat tagccatcat
     4741 tcttctgatc ctttctgacc tacgacttta cgttcaccgt gcaccagttg cgaactccag
     4801 ctcaccacca gaaaaaccac cagtgtactg acagcacagg aaaccactac cgggccaaac
     4861 tgtgcgcgga gcaaatcaaa atattgcatg acgcctacgc caatcggcac aaacaatagc
     4921 gccatatagc gaatcagtac gtagcacccc ggattgaccc attttgccgg aagaatttgc
     4981 aaggccagca ggacaaacag gatcagcatc ccgatgatgc tgcccggaat ggttaccggt
     5041 agcagggaag caatgaaaat gcctgcatac aggcaggcat aaatcaggac gaaagcgcgt
     5101 aaatattgcc agataatgtt cagtgtctta ctcataatca acaccccaga tgagatgaat
     5161 tcatcataca attaattcgt aaaacgtgct accgatcaca ttaagaaatc agcgcttatt
     5221 ttttcgctcc ccggcattac gcaatgcgca acgaactcag gcatacatca agcatagaga
     5281 gggattacag tttgcagcaa tccctctttg cagaccttta atccgttgac cataggcgct
     5341 aacatagcgc ctcatttttt tgcgggtgaa gatatgcgtg tgttactggc tccgatggag
     5401 ggtgtgcttg actctctggt gcgtgaattg ctgaccgaag ttaacgacta cgatctgtgc
     5461 atcaccgagt ttgtccgcgt ggtggatcaa ctgctgccgg taaaagtttt tcatcgcatt
     5521 tgccctgagc tacaaaacgc cagccggaca ccatccggta cgctggtgcg cgtgcagttg
     5581 ttgggtcagt tcccacaatg gctggcagag aatgccgccc gtgcggtgga attaggttcc
     5641 tggggcgtgg atctcaattg cggctgcccg tcgaaaacgg tcaacggtag cggtggtggg
     5701 gcgacgttac tcaaagatcc tgaactcatc taccagggtg caaaagcgat gcgtgaagct
     5761 gtaccggcgc atttgcccgt cagcgtgaaa gtgcgtctgg gctgggacag cggtgagaag
     5821 aaatttgaaa tcgccgatgc ggttcaacag gctggtgcta cggagctggt ggtgcatgga
     5881 cggacaaaag agcagggtta ccgcgcggag catattgact ggcaggcgat tggcgagatt
     5941 cgccagcggc tgaatattcc ggtgattgcc aacggtgaaa tctgggactg gcagagtgcg
     6001 caacaatgta tggcgatcag cggctgcgac gcagtgatga ttggtcgcgg ggcgctcaat
     6061 attcccaacc tgagccgggt ggtaaaatat aacgaaccgc gaatgccgtg gccggaggtg
     6121 gttgctttgc tgcaaaaata tacccgtctg gaaaagcagg gcgataccgg gttatatcac
     6181 gttgcgcgga ttaaacagtg gttgagttat ttgcgtaaag aatacgatga agcaacggaa
     6241 ttatttcagc atgttcgggt gttgaataat tcccctgata ttgcaagggc tattcaggca
     6301 attgatatcg ataagctgcg ataatagtgg aatcgcagtt tatcagagat aatgaatcgc
     6361 ttattcagct tcatcatctt gttgcgctcc tttcatgagc taagccacat attgccactg
     6421 gcgcaaggag cgcgcagggg gaggacaatc gcctcctccc cctgccccct gggctctggc
     6481 gaacaaaatc gccgctgcgc ggtaccttca gcttatcccc ttcggctacc gggtcgggcg
     6541 cgaggtaacg tccttgtaag acgcgccctc agcccacatc catgtgggct gccccggcct
     6601 tcggggaacg cttcagcgat tttgacgcca ccaacaccgg agctgtttgc aaatcaagta
     6661 cacaaaatat ctagttcctg acctgacaac ttatatgcca tatctgattt ttaaaatatc
     6721 atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa tacagcccag
     6781 agtataattt tcattggcgt tcctttattt gtgagtgttg aataagtata ggaaaaaacg
     6841 aagccctttg ccgttatcca taaacgatta agataaatcg gaagatatca ttcacttagt
     6901 ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc gaaactaaaa
     6961 aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta ttcccccatg
     7021 aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttattgat gctggccggg
     7081 tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc tgcacaagtt
     7141 gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg gtggctggag
     7201 tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc accggatatg
     7261 caggtggctg aacagcgcat tcagctagct gaagcccagg cgaaggcagt tgccactcag
     7321 gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc ggcagaaggc
     7381 ttaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggccc gtggtacacc
     7441 aacggtactt ttggcttaac ggcgggctgg catctcgata tctggggaaa gaatcgggcg
     7501 gaggttactg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga gcaaacccgc
     7561 caattgctgg ctggcagcgt agcccgcctg tactgggagt ggca
//
LOCUS       DAJVOY010000184         7464 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVOY010000184 DAJVOY010000000
VERSION     DAJVOY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.184"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..341
                     /locus_tag="OQL90_003519"
     CDS             <1..341
                     /locus_tag="OQL90_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1370169.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            716..1150
                     /locus_tag="OQL90_003520"
     CDS             716..1150
                     /locus_tag="OQL90_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS1370170.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1161..1802
                     /locus_tag="OQL90_003521"
     CDS             1161..1802
                     /locus_tag="OQL90_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370171.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1799..2149
                     /locus_tag="OQL90_003522"
     CDS             1799..2149
                     /locus_tag="OQL90_003522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370172.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRIETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2843..3454
                     /locus_tag="OQL90_003523"
     CDS             2843..3454
                     /locus_tag="OQL90_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370173.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELSVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3992..4534
                     /locus_tag="OQL90_003524"
     CDS             3992..4534
                     /locus_tag="OQL90_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCS1370174.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4527..4886)
                     /locus_tag="OQL90_003525"
     CDS             complement(4527..4886)
                     /locus_tag="OQL90_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370175.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5320..6144
                     /gene="dinD"
                     /locus_tag="OQL90_003526"
     CDS             5320..6144
                     /gene="dinD"
                     /locus_tag="OQL90_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1370176.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6436..7053
                     /locus_tag="OQL90_003527"
     CDS             6436..7053
                     /locus_tag="OQL90_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1370177.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7050..7464)
                     /gene="ligB"
                     /locus_tag="OQL90_003528"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7050..7464)
                     /gene="ligB"
                     /locus_tag="OQL90_003528"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         1998 a         1831 c         1907 g         1728 t
ORIGIN      
        1 agcagacgga ggcgtttttg aatgcctatg ggctgagtcg attcgcgccg ctgggttatg
       61 acccgcgcga tttacccatc cgtgatttag ccgggtacag gaagaagggg aaccacgacg
      121 gcgacccgat aattttctac accttcccgg cagcgtttga gcaggagatt gcaaagggct
      181 tcaataccaa acagtttgcc gaagttctaa agaatgcagg aatgcttaca ccaccaacga
      241 gcgggcgagg ctatcagggg cgagtaaggg aggatgggcg acagattcgc gtctatgtac
      301 tcaatttcat ggcagaggaa agcagccagc cagaagaata attatttctt cacatgcgta
      361 gttttagtgt tgtctcggtt gtctttgttg tctcaatttg taattaagat tgatttataa
      421 ggattttatt ttaatttctg agacaacacc gagacaacat ttagccattt tgagacaaca
      481 gggcgaaagt gtgtttttag tctggcaggc agtgagacaa catttatgcc gggtgagaca
      541 acacgaaatt gcaaatgttg tctcagcaaa acccagacgt ggcgcggcct gtagagccgt
      601 gagacaacaa agacaacaga gacaacacta tttttgctta ctatgaaaaa aaatcggcag
      661 gtgtcccaca ttaagaaatc gtcattagca aaactaataa tccagtgagg ggaaaatgac
      721 agcacaaatt tcagcatacg ggcggctggt ggcagatccc cagacccgca ccacaacgaa
      781 cggtaacaac atggctatgg cgcggctggc ggtgtctctc ccctgtaatg cggcagaggc
      841 gggagagtca accttctggc tgggcgttat tgcttttggg aagcaggccg atgcgctggc
      901 aaagcaccag aaaggcgacc ttgtgagcgt ggcaggcaat atgcaactca accagtggac
      961 agggcaggac ggcggtacac agcaggggta tcaggtagta gccgacagtg tgatcagcgc
     1021 cagaacggta cgccccggcg ggaaggcagg acagcgggga caggcaacgg acgcgctgag
     1081 acgtgcgcag cagccatccc gtgatgaata cgaccaacgc ccaccattcg acgatgaaac
     1141 gccattctga gagggaagat atgagtacgc aacccatcat tattgagttc ggcagctatc
     1201 gctggcccag acatcttgca atacatgaag cagatcatgc gatagccgca tggtttttcg
     1261 gcatacagga ggtagaaatt gcgttatcgg gtaataaccg agtggcaaaa acctacctta
     1321 gcggcgatgt tgatgaatgt ggagctgtcg cggtatggca cgccaactat ccacggaatg
     1381 tggctgaggt taagtcttac accctctcga atgatgacac caggttacgt ctgatagacc
     1441 aggtatgccg ggctatggtt atctctctgg ctggtatcgt cgcgcaatca cgctatacgg
     1501 gggatgatat taacgcactg atggaaacgt ccgggaaagc agacatgaaa aatgttcgtg
     1561 atttatcaga ggtataccgg gtggctggtg gggaggatgc tgatatacag agaatatccc
     1621 tttcccgtgc tgaggcgctg attgccctga aatggtggga tgttatcgcg ctggcgaaaa
     1681 tactggagaa ccgtaaccat atgcctgcca gcaacttcca gcgcattatg ggtgatattg
     1741 acaagcctgt accaaagcct atgacgctgg ctgaactgga tgaattggtg ggtgaagaat
     1801 gaagccatac attctggcag ccggcctgat ctcctcggca ccggcactat ctgctatcca
     1861 ttgtgggggc tttaccatac agccagaccg ttatcggaca acgattaacg gcgatgtggt
     1921 tcatattgtt gatgcgcaat tttacggcgc accgcgagac tttagccagg ccgccataaa
     1981 gttattgccg gatgccatta ccactaccga taggcaatac cgcatagaga cgcagggagg
     2041 caatgccttg cttgagttga tgaccagaga aaacccgcca cgtgtgctta atcgtgagcg
     2101 ctgcgatgcc gggttaacaa gtttcggttg gtacacttct gcgccgtgat gttcgttccg
     2161 agcgcagcct aaaatattgg actgttcttg acggatttaa aactctaatg gagccaagtc
     2221 cttgccagta gcgggctagg agcctaattg tgattcaggt cgtcaaaaat attcaggttt
     2281 gcggcattgg ttcgcagtac gcagtccggt acgcggcaaa actgcgcatt taacgtgcgt
     2341 accacctgac gaattctaac gccggcattg caaacagatg cgtgcgcaaa atggctaaga
     2401 cgtctgatat tgctggcttt ggctcaatca gtttggaggt ggcggtatca gtacgatgct
     2461 aatttttgct aaggttttag tacgcaactc aggtacgcac tctcaccaga tacgaaaccg
     2521 attcgcagtt gcgtaccagc ctgtaaggcg cattatcgct gggtttctac agtcgaatgc
     2581 agtacgcaaa gtggaaaatc ctccgaatac gaaacccgag cattgcgcag taaattgcga
     2641 attgcgcagt taacaaaata tctccgcaaa acgaaacgga tatcaatgcg caaaccatcc
     2701 tcaacccccg cgcggttact ggctttattg atgtgaaatt gcaaaattgg tgcgcaattt
     2761 acagtaaaat acgcatatta tttcgtattc aatttgcgca ccgttcgcag ttaatgcgca
     2821 gtaattcgca ggaggccagc cagtgagaga cagaacgcgc agctatacca ccgacctgcc
     2881 ccgtataggg ttgccattcc ttgcgaacat gcgtagcagg ctggcggatg ctgaaccggg
     2941 tacgcagctt tatacccaga ccgaaagcgg aacgttgtat gccttccggc aagccgacag
     3001 ctacgccatg acaatcaacg gcgtgacgcg ggctattcgc accacaacca cgaaggccgg
     3061 gtatggcgtc cgggaatggt acgcttgccc ccactgtatg aagcgggctg caaagctgta
     3121 tatcggcaag aaagacattg gctgccgggc gtgctggaaa cttcactata aaagccagag
     3181 tgccgacata ctggacagga tgcgcatgaa gatacgccag cagcgatgcg cgatatgggg
     3241 cagcaatgac cttgccaata atctgtttaa tgacattcgc atgttcccaa aaccaaaggg
     3301 gatgcgctgg gctacgtttg acaggaagcg ggcagagtta tcagttatgg agatggctta
     3361 ctggcaggca ttcagcccgg tagtggacag aatcacgggc gttattgagc gtaaagcagg
     3421 aaaagcagcc agaggcattg gcctgactct ataaaagcaa aacgcccgca cggggcgtca
     3481 gagaacggga tagacttctg cgacaatttg cattcgccgc caatatagca agccgtccac
     3541 atcaagcaat aaactatcac cgggaggggg aggccaaatc tctgcaatcc tgagccttaa
     3601 ggactgcccg ccccatcgtt ttttcatacc cgcgaaaaat gaaatttaat ccgggcgtgt
     3661 ttcatccttc cagagggtat gacgtatgac accacgcgaa atagccttat tgaccatcgc
     3721 caaacttgag catggaggcc accagcttac acaggcagat caacgggaga tagaacgctc
     3781 agttaatgcc gatatagccc ggcgcgacag gttccgcgaa atgatgcgag cacctgccta
     3841 ccagtggaag aagccagcgc cgcgcaggta gatacgggat tattccgcat gtaacttatg
     3901 cgtattaagc aaatttcctt tattcacctt tacagaattg tgagtacgtc agtatcgtta
     3961 tccttccgat tcgcatttat tcaggatttg catgtaccag ctaaagataa ccatccgaga
     4021 cagcaaacca cctatatggc ggcgtgtgct ggttccagag caaatcccct ttagtgaact
     4081 tcacgcggtg atccagttgg cttttggctg gaatgacgaa cacctgtata tgttcgagaa
     4141 agggcgtaaa ggtgatcccg gtagcgagta tcgcgtatgg ggtgaggatg aaagcatggg
     4201 taatgcggca atcacaccac tatgggcagc gctccagaat gatggtgaca agctggttta
     4261 cacgtatgac tttggcgact ggtgggattg cgtcattgtg ctggagaagc aaacacacga
     4321 tacgagcaat cagcccatta gctgcctgcg tgggaaaggc accaccccgg tggaaaactc
     4381 aggcggcttg catggttaca atgaattgct attacaggcc agagagtctg ataatcctga
     4441 gcaggccgag atccacaatt tcctgatgct ggatattgag cgccgggttt acgacctgag
     4501 cagcatcaac gacagattgc agactcttaa ctgacccgca tatattcgcc tgatattgcg
     4561 ttatcagcgc cgccagtatc gcgcataaca gcgcactgac cgggcaaacc gtagcgcacg
     4621 accgttgcgt tagtctcacc gccgcgccgt atctggcgcg attcatacac tgtcacccgc
     4681 tcaagcagtc caccagcaac catgctttcc agtgtgcgcc gggttgattc gagctggtga
     4741 cgcttatcga acgacaccat gccatgaagc aggtaggcca cacccgacac atcgaacggc
     4801 ggcgcaccaa tctcaccagt cacccattcg aggttatccg gttcaaagta gctaagtatc
     4861 tcttttttgc ggctggtcat tctcatagct ggcaggttcc ttattgtggg atagcactat
     4921 catacaataa ggcgcaaaag ataagaggcc agaacagcaa gcatgagagc ataaaacgag
     4981 cagccaaaga cagcgcctca aaataccgtg tacacaaggg tgtacctact gagaaaacgc
     5041 attacaagaa acacagcaac cacgggcgtt taaagccact actcaataaa cagattcaac
     5101 tcgtaacgcc aattcttact tttcagcctt cacaaatgcc gcccactcaa acagagcggc
     5161 atttttcttc cccgcaactt caattctgtt ttgcgtgcct gctccagatt ttgcgatgtt
     5221 tttttgccca gcacactgag aacgtgagat actcacaact gtatataaat acagttacag
     5281 atttactttc tttgcaatcg atatcacatg gagtgggcaa tgaacgaaca tcatcaacct
     5341 tttgaagaaa tacggcatta tggcactgaa gggcaagaat tttggtcagc tagagaattg
     5401 gcaccgttac tggattatcg ggactggcgc aattttcaaa aggtactggc ccgcgcgact
     5461 caagcgtgcg aagccagcaa tcaagctgca tctgaccatt tcgttgaaac caccaaaatg
     5521 gttgtattag gttcaggggc tcaacgagaa cttgaagatg ttcatctctc ccgctatgcc
     5581 tgttacctgg tagtacaaaa cggcgaccct gcgaaaccgg ttattgcggc agggcaaact
     5641 tattttgcta tccagacccg acggcaggag ctggctgacg atgaagcttt caggcaactt
     5701 cgtgaagacg aaaagcgtct ttttctgaga aatgaattga aagaacataa caaacaattg
     5761 gttgaggcag cgcagcaagc tggggtagcg acagctatcg atttcgccat atttcagaat
     5821 catggttacc gggggctgta tggtggatta gatcagaaag ctattcatca gcggaagggg
     5881 ctgaaaaaga atcagaatat cctggatcat atgggttcaa cagaactggc ggctaatctc
     5941 tttcgagcta cccaaacaga agaaaaactc aagcgggatg gcgtgaattc aaaacagcaa
     6001 gcaaacacca cacactttga cgtgggtcgc aaggtgaggc aaaccattca ggaacttggc
     6061 ggaaccatgc ctgaggagtt gccgaccccg caggtcagca tcaagcagtt ggaaaacagt
     6121 gtaaaaatta cagagaagaa atagcagaaa ctaaaaactc tttttcttaa ttgagatacc
     6181 cgatgcgtaa gccaaagtcc aggtgcagtg aacacaatgg ctaaacattg cacctttctt
     6241 ttccacccct caattttaac ctattttttc ttatgcattt tctcagacaa gaactcagaa
     6301 taatgcatct acgctacaga aaatagcgat ttcacataac tacaagttat caatttcctc
     6361 ttaccttaaa aaatctcaat cgtgacaatg cgcacaaatc gctaccctgc cagacagatt
     6421 tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcatcac tgccgaagcc
     6481 atgacggggg cgctggcggc cggacgacgg cgcatggata catttggcgt aattattatt
     6541 gcgaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg ccactatccg
     6601 ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc agtactcacc
     6661 actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct cgatgcgctc
     6721 ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg gcacggccca
     6781 attattgccg ttgtcgcggc ggtaaccacc ggcgtgtttg gcggcgtttt gcgcgatatg
     6841 ttctgtaaac gcatcccact ggtattccag aaagagttgt atgctggtgt ctcatttgcc
     6901 tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt ggtgattatc
     6961 tccaccctgg tattcggctt ttttgcccgc ttactggcgc tacgtcttaa gctgggatta
     7021 ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat ctgctgggca
     7081 gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat aacctgtctg
     7141 gcgcgcccag atccagtccc cggcagctgc tgtcagaact gctccgtgct aaataaaagt
     7201 tgcgactagg accgttcatc actggcatta agcgccgccc gggttagcgg tattcccatt
     7261 gccatcaccc agcgagtaaa aggctgctta cgagccagat taaactgatg ccataactgc
     7321 gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt taataaaagc
     7381 caagaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc ggcctcacca
     7441 atgccatcca gcccaagaac ctgt
//
LOCUS       DAJVOY010000185         7277 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVOY010000185 DAJVOY010000000
VERSION     DAJVOY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.185"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            198..3611
                     /locus_tag="OQL90_003529"
     CDS             198..3611
                     /locus_tag="OQL90_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1370178.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            3675..5309
                     /locus_tag="OQL90_003530"
     CDS             3675..5309
                     /locus_tag="OQL90_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1370179.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5318..6388
                     /locus_tag="OQL90_003531"
     CDS             5318..6388
                     /locus_tag="OQL90_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCS1370180.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6513..7229)
                     /locus_tag="OQL90_003532"
     CDS             complement(6513..7229)
                     /locus_tag="OQL90_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370181.1"
                     /translation="MLSETKKRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEE
                     GVSTTEFVVGYGAISMVKKSESMATKGLVGLERVDLIREVVFENGYYDWGCVVNDVLP
                     NICKGNARIPIFHFLNHAKLINPDGSIINETELSGGVLSRLNITPISFQSQGWDKRRS
                     ETVPEVKVGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDEAMNTQVL
                     SSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2096 a         1675 c         1714 g         1792 t
ORIGIN      
        1 ttagtgagca aggtatccct tgagtagcca gggctgaaga cgcccattga tgggcgaaaa
       61 ccaaggttct ggccgttaaa gcggctatgt cgttaaacac atcaccacca tatgccatcc
      121 ggctcacttc gtcggccaaa ttagtgtgat attctttcca gaatttctgt tgtatccaca
      181 acataaggcg atgattgatg gagcagtctc tcaattttga aatgttgcgc agccagtggc
      241 cggaactggc agaactcgca tgtatggcag agcgctatgt tcactccgat ccggaaagct
      301 gcctggttaa gctgcgcaac tacaccgaat tgatggtgcg ctggttgtat cgtcaggagc
      361 ggttgccgga aggtattaag gctaatcttt acgatttaat gaacgctgat gtctttacca
      421 gcatgatgcc ggaagccatc atcatgaaaa tggatgcgct gcgtatccat ggcaaccgtg
      481 ccgcgcacgg cggacgtatc aaagctaaag atacttactg gctgctcaaa gaagcgtatt
      541 tgttgggaat ttggctgtat gttcgctacg cccacggtaa tgttgatgac tgccccaaat
      601 ttacactccc tccattaaca caatcttcag gtcgtgcaga tgaaaaacgt ctggaagatg
      661 caatcaaggc tcaggatgaa agccgtgagc gcgaactggc gctacaacgc gcactacagc
      721 aagaacagga aaaggccgaa cacctcactc aacgcctgaa tgaagccaga gcgcgtaacc
      781 agcatgttgc cgatattctc tctattgatg aagcggaaac ccgccgtcgc ctgattgact
      841 ctcgcttact tgcagccgac tggaatgtag gggaagaact taaaaacacg gatcaagtca
      901 cacaagaaca tccagtcaaa gagcaaccca ccacctctgg tgacggttat gcagactatg
      961 tcttgtggga tgaggcacac aaaccgttgg cggtggtgga agcaaaaaaa accagcgtca
     1021 atgccgagca aggacgaatt caggcccggc tgtatgcaga ctggctggaa aaagaatacg
     1081 gtcagcgtcc ggtcatcttc tacaccaacg gctatgatat ctggctgtgg gatgaccata
     1141 aaactcatgg ttatcccccg cgtcgggtgt ttggcttcta cagcaaggaa agcctgcaat
     1201 atcttattca gcagcgtgaa acccgtctgc cgctgaacag agtgccacac gtaaaagata
     1261 acgaaggtaa ggccgttgcc ggacgtttgt accagctcga aaccattgcc cgcgtcagtg
     1321 aacggtttac caacaagtac cgacaatcgt taattgttca ggctacgggt acaggcaaaa
     1381 cccgcgtggc aattgcacta agcaaactga tgattgatgc ccgctgggta aaacgcgtcc
     1441 tgtttctttg cgaccgtaaa gaactgcgta aacaggcggc aaatgccttt aatcaattca
     1501 ccaatgagcc gctgtatgtg gtcgggaaat cgaaaaaagc agacaggcag aatgccagaa
     1561 tctacatcgc tacctatccc ggtatgatga aaatcatgga ccatttcgat gtcggttatt
     1621 tcgatttgat catcgccgat gaatcccacc gctctatcta caacgtctac ggcgatctgt
     1681 ttaagtattt tgatgccctc cagattggcc tgacggccac gccaattgac atggtgagta
     1741 aaaccacttt cggcctgttt ggttgtgaag gacgtattcc taccgccaac tacagcctgg
     1801 aagatgccat cgccgataac aatctggttc cctatgaggt tgtcacacac acaacagaat
     1861 tcctgcgcga aggtatcaaa cgggagaaat taagcgacgc acaaatccgc gagctggaag
     1921 aacaaggcat agatcccaat acgctggagt ttgatggcaa ggcgctggat gaggcgatct
     1981 acaacaaaga caccaaccgc tatatcctgc gtaacctgat ggaaaatggc ctgaaagatc
     2041 gggacggcca gttgcctggt aaaaccatca ttttcgcccg taaccacaaa cacgcgctgc
     2101 tgttgaatga attgttcgac gatatgtacc cacagtttgc cgggcgcttc tgccaggtca
     2161 tcgacaacta cgatccccgc gccgagcaac tgattgatga ttttaaaggg ctggatgaaa
     2221 gcaccaacaa agagctgacc atcgctatct ccgttgacat gctcgatacc ggtattgatg
     2281 tcccggaaat tgtgaatctg gtctttgcca aaccggtcaa atcaaaagtg aagttctggc
     2341 agatgattgg ccgtggtacg cgcctctgtc cggggttgta cggttacgac gataacggca
     2401 aaccgctgga taaacagaaa ttccgcattt tcgatcactg gggcaatttt gagtatcacg
     2461 agctgcatac cgaagaggcg gaagtcacag cgacaaaatc gcttgcgcaa aaacgcttcg
     2521 aagcgtgggt tatgctgggg gccgctgcgc agcgtaaatt cgacaaacag gcggtggatt
     2581 tagttgctca ccagcttcgc gagcaaatca acgcactgga tgaaaagtcg attgccgtac
     2641 aggaaaaatg gcagcagaaa gcgcagtaca gtgatgaaaa ggtgctgcgc cagctttctc
     2701 cgaaaacaca gcaggatctg ctatctgttc tggccccgtt gatgcagtgg ctggacgtgc
     2761 gcgggcaaag cgatgccata cgctttgata tggatattct ggcggcgcaa actgcccgtt
     2821 ataccaaccc ggaagagctg gatgttctct ggccgatcat cgtcgaaaaa gtggagcgcc
     2881 tgccgccgca tttggcgcag gtgcagcaac agggacaacg aatcaatcag cttcgtaatt
     2941 taagctggtg gaagcaggcc agcctggaag agctggagga tattcgcatt catttgcgcg
     3001 gtatcatgca cctgatggaa aaagacgcga cgcctaaatt tggttcgata caggtggata
     3061 ttaccgaaga tgcgaacctg atccagacgg agacccgcaa aactaatatc cgctcgattg
     3121 atttcaaact ctatcgccag caggttcagg gggcgctgga gccgctgttc cagcaaaacc
     3181 cggttctgaa gaaaatccgc aacggcgagc cagtcacgca aaacgagctg gatgagctgg
     3241 cgaagctggt gctgatccag aaccccaacg ttgatattcg tgcgttgaaa gagttttatc
     3301 cgcaggcaac cgccagcctg gataaacttt tgcgtaccat catcgggatg gacagcgacg
     3361 cggtggaagt gcgttttgcc cagttcgccg ctgataacag cctgaccagc caacaactgc
     3421 gcttcctgtc attgctgaaa aaccacattc gcgattacgg caccattgaa atgcggcagc
     3481 tctttgaaca gccttttacg catatccaca acgaaggcgt taccggtgtg ttccctgata
     3541 tagcgcaaat cgcccgcctg caaaagatag tcgaagagct gggtgttgtg accgacgcag
     3601 cgacggtata atacacgcat gacaagcccc ggcagcgggg cttttgtatt taaaacagga
     3661 tataaacgct aacaatgatt actggcgact taaaaagtaa aatcgacgga ctatgggaag
     3721 atttctgggt gggtggtatc accaacccgc tgaccgtaat cgaacagatc acctatctga
     3781 tgtattcccg gatgctggat acccaggaac aacgcgacga aaagcgtaaa caaatcgcgg
     3841 gtattgattt taaaccgcgt tttgcgccag aacagcagga gttccgtttc agtcactata
     3901 gcaaccttgg ctcggatgag atgatggaag tggtgcgcga tggcgtattc cagcatttcc
     3961 gtcagctcgg ccaggccgat gcttcgaagg tgacgctgct gggcaacttt atgaaagatg
     4021 ctcgtctgga gattgttaag ccgtcattgc tgaccaaagc ggttgaggta atcaaaaacc
     4081 tgccattgga tcgcggcgac accaaaggcg acctttacga atacctgtta agcaagctga
     4141 caactgccgg aatcaacgga cagttccgca caccgcgcca cattatccgc acgatggttg
     4201 aaatgatgga gccgaacccg gcccgcggcg agacgatttg cgatcccgcc tgtggcaccg
     4261 gtggttttct ggcaaccagc tatgaatatc tgctggagaa gtacagctcg ctggaatcca
     4321 ttcatactga gattggcacc aacgaacgtg gcgagctgga agagcaaaaa atctttaccg
     4381 gcgatctgct gacaccgtgg cgtaaccatg tggataacaa catgttccac ggttacgact
     4441 ttgacaccac aatgctacgt atcgccgcca tgaacctgat tatgcacggc gtggatgcgc
     4501 ctgatatcca ctatcaggac acaatgagcc agagtttcag cacaaacttc ccgcaggcca
     4561 gtaaaaacgc cttcaacctg attctggcga acccgccgtt taccggttca ctggacgagg
     4621 aagatatcga ctccacgctg tcggcaatgg tgaaaaccaa aaaaaccgaa ctactgttcc
     4681 tggcgcgtat tctgcaaatg ctgaaagtgg gcgggcgcag tgccactatc gtgccgcagg
     4741 gcgtgctgtt tggctctagc aaggcgcacc agtcacttcg caaaacgctg gtggaagata
     4801 accaactgga aacggtgatc aatctgcctt ctggtgtatt taaaccttac gctggcgtgg
     4861 cgacggcgat cctgatcttt accaaaggcg gtcaaacgga tgaggtctgg ttctacgatc
     4921 tacaaaatga cggctacagc ctggatgata agcgcaaccc gataaaagac aacgatctgc
     4981 cgcatctgct ggcaagctgg aagcattacc gtactttacg cgggctaccg gttgataact
     5041 ttatgggtaa gaagttagcc tcgttgctta aacagcagta cccggaaggg attaatgctg
     5101 gcgttgattt taaagatcgc acgcaggcgg cgtttgttgt accgaaagcg gatattgctg
     5161 cgcagaaata cgatctatcc atcaaccgtt ataaagaagt cgtgtatcag gcggaggaat
     5221 atgaagatcc gaaggtgata ttgaagcggt taaaggatct ggaaaaagag attctggcgg
     5281 atttggatga gctggagggg atgctgtgag ttcggcgatg gtgaagttag gggatgttat
     5341 taatgttcac tacggaaaag cattaaaagc tgatcagcgt gtatcgaatg gaagtgtcca
     5401 tgtgtttggt agttcaggaa ttgtcggcaa ccacgataaa acgctttgca gctatcccac
     5461 gataattatt ggccgcaaag gcagcgttgg tgctattacc tgggctccaa gcggaggctg
     5521 gatcattgat actgcttatt acgttgagat caaagataat aataagttag atctccgata
     5581 cttgttttat attctgagcg gaattgatct cactaagaaa acgattacca caagtattcc
     5641 tggattaaat agagacgatc tctatgatac atttattaaa ttaccacctt ttgaagagca
     5701 gaaacgaatt gtggatcttc tcgataaagc tgagggtata cgccaaaaac gtgagcaatc
     5761 catcaaactg gctgatgatt ttttgcgtgc tacgtttgct acaatgtatg ggaatcctat
     5821 tacaaacccc aaaaaatggc ctgtccatct tatgggagaa attattgaat ttaaaggtgg
     5881 aaatcaacca cctaaaagtg attttatttt tgaacctaaa cagggttata ttagattagt
     5941 acaaattaga gattttaaat ccgataaata tgcgacgtac attccgcaag aaaaagcaaa
     6001 acgtattttc gaagttgatg atgttatgat tgctcgttat gggccacctg tgtttcaaat
     6061 acttagaggt ttatccggca gctacaatgt agctttaatg aaagcttcac ccaaagaaaa
     6121 tattaggaag gggtttatat tctatcttct acaacttccg gaatatcatg atgttgttgt
     6181 aaaaaactct gaaagaacag caggacaaac cggtgtaaat cttgaactat taaacaaatt
     6241 caatgttccc ctcccaccta tatactatca agatgagata ttagatagat tagctagaat
     6301 tgaaaaattt aaagaaaaaa tagaaatatc attaaatcat ttagaaatac aatttttatc
     6361 actacagaag agattaatag gtttttaaat ccttagtatc gaaataaaca ttaatttatt
     6421 atattaaatt aagatatttt aggaaattaa ggcgtcactc ttaggtaacg ccttttttta
     6481 ctatctcgcc ccctaagaag gttatatctt acttaatacc tgtgactatt tttgatataa
     6541 tcataaatac acactaattt gcaaaaatta cttgacaata cctgagtatt catagcttca
     6601 tctatatgtt ttttcaatat tttgagaagc tctcccatat cgcgcttgat taatccaggc
     6661 tccatataag gcatcattct taaaaagaaa ctgaaatcat aagcaagata taactcattg
     6721 acccccacct tgacctcagg aacggtttca gagcgtcgtt tatcccatcc ttgagactga
     6781 aaactaatag gagtgatatt taatcgtgat aaaacgccac ctgagagttc tgtttcatta
     6841 attatactcc catcaggatt aattaattta gcgtgattta agaagtgaaa aataggaatg
     6901 cgcgcattgc ctttgcatat atttggcaaa acatcattaa caacacatcc ccaatcataa
     6961 tatccatttt caaatacaac ctctcggatg agatctacac gctccagtcc aaccaatcct
     7021 ttagttgcca tcgattccga tttttttacc atcgaaatag caccatatcc cacaacaaac
     7081 tcagttgtac tgaccccttc ttcaattttc tctgaatccc tcacatataa ttttcctttc
     7141 ggatcatcag ttaagaccaa ctcataaagt tcatctttta tcattcggaa taaatgtgct
     7201 ggaatccttt ttttagtttc actcagcacc tcaaatattt ctttatagga tggtgcctga
     7261 acatatttta ccgggat
//
LOCUS       DAJVOY010000186         7215 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVOY010000186 DAJVOY010000000
VERSION     DAJVOY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.186"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            600..884
                     /locus_tag="OQL90_003533"
     CDS             600..884
                     /locus_tag="OQL90_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1370182.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            872..1357
                     /locus_tag="OQL90_003534"
     CDS             872..1357
                     /locus_tag="OQL90_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1370183.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            1391..2569
                     /gene="ltrA"
                     /locus_tag="OQL90_003535"
     CDS             1391..2569
                     /gene="ltrA"
                     /locus_tag="OQL90_003535"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1370184.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2652..2806
                     /locus_tag="OQL90_003536"
                     /pseudo
     CDS             2652..2806
                     /locus_tag="OQL90_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2860..3342)
                     /locus_tag="OQL90_003537"
                     /pseudo
     CDS             complement(2860..3342)
                     /locus_tag="OQL90_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3598..4578)
                     /locus_tag="OQL90_003538"
     CDS             complement(3598..4578)
                     /locus_tag="OQL90_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCS1370185.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4578..5777)
                     /locus_tag="OQL90_003539"
     CDS             complement(4578..5777)
                     /locus_tag="OQL90_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1370186.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(6099..6437)
                     /locus_tag="OQL90_003540"
                     /pseudo
     CDS             complement(6099..6437)
                     /locus_tag="OQL90_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6504..>7215)
                     /gene="istB"
                     /locus_tag="OQL90_003541"
     CDS             complement(6504..>7215)
                     /gene="istB"
                     /locus_tag="OQL90_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCS1370187.1"
                     /translation="AAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMYTRMAA
                     FPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGYE
                     AVRAGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQKEA
                     KLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKGES
                     YRLRQKRKAGVIAEANPE"
BASE COUNT         1918 a         1644 c         1729 g         1924 t
ORIGIN      
        1 ggcggcatcc ggcatatagg cagcatgaac gcaacacagg ctctcgtacg gaacgtggga
       61 acctgtcgtc tcgatgctaa gggagcggcg cggagcagag gcactgcaag cgcctgagta
      121 ccgatgcgag acacaggggc agatggcttc gtagtagtga ggaagtgcct gtaatgggca
      181 tggagcgaag ggggtcagtc gaggcgtttg aggttaccaa tcgaagctat acccggtaat
      241 ggcgcaggtt gacctgcact tggccaagtg gattgcccgc aaacacaagc gagcgcgagg
      301 tagtctggtg cgtgcattcg agtggttact gcgaattcgt cacgactgcc cgacgctgtt
      361 tgcacactgg tgcttagctt acgatacttg aatggttcaa cgacaagagc cgtatgacgc
      421 gagagtgtca cgtacggttc cgtgggagcg taggggggcg gtgcccctgc gccacccgat
      481 atcccctttg agtgcggtta cgtccacaga ggtaaatgcc gctgacgcgg catctgtaat
      541 ctgttacttt gtgtacatca tttgctatat attgtcaata tgctattgtt gtaaagcaaa
      601 tgatatacgg aggctttatg aaatcaggtg ttcagctcaa tctcagagca agagaatcac
      661 agcggatact cattgatgct gctgcagaaa ttcttcataa gtcgcgtaca gatttcattc
      721 tggaaatggc ctgtaaagct gctgaagatg ttattctcga ccggcgggtg tttaatttta
      781 atgacaggca atatgaagag ttcattgaaa tgcttgatgc tccggttgca gatgatcctg
      841 ccatcgaaaa attgctggca aggaaacctc aatgggatgt gtaactgcac cagaaccttt
      901 atcctcattt catcaggtcg ctgaatttgt cagcggtgaa gctgtacttg atgactggtt
      961 aaagcagaag gggctaaaaa atcaggcaat cggagccacc cggacttttg ttgtatgcag
     1021 aaaaggtacg caacaaatag tcggttttta ctcactggcc actggtagtg tcaatcatac
     1081 agaagctaca ggtaaccttc ggcgcaatat gccagacccc attcctgtaa ttatacttgc
     1141 acgcctggct gtagatgtgt catttcgagg aaaagggctt ggcgcggatt tacttcatga
     1201 tgcagttcgt cgttgttaca gggttgccga aaatatcggt gtgcgtgcaa ttatggttca
     1261 tgcgcttacg gaaaacgcaa aacagtttta tattcatcat ggttttaagc catcaaaaac
     1321 tcaggtgcaa acattgtttc ttaaactccc tcaataggga gtaatgcact ggtctgggca
     1381 tcatatctgg atgtgcgtcg caacaaggga gccccccgga tgcgacgggc agactttgaa
     1441 aatgttcgac cagcaacgtg atggcaatct atacaagatc tggaaccggc tatgttcggg
     1501 aacatggttt ccgccaccag tgctggaaaa acggatcccg aagtcgaatg gcaaggaacg
     1561 cattctgggg atcccaacag tatccgatcg aattgctcag ggagcgataa aacttttcat
     1621 ggaagaaaag cttgacccga ttttccatgc tgattcatat ggttaccgac caggcaaatc
     1681 agctcatgac gctctgaaac aatgtgccat ccggtgctgg cgttatagct ggatactgga
     1741 agtggacatc agtgcgtttt tcgaccatgt gaaacatgat ctggtgctca aagctctgga
     1801 acaccacggg atgcctaaat gggtcatcct gtactgtcga cgctggatgg aagcgccaat
     1861 gcagagttgt gaaaatggag aattaataac ccgaacacga ggcactccgc agggcggggt
     1921 cattagtccg ctacttgcta acttgttcct tcactatgca tttgatttgt ggatggaaag
     1981 agaatatcgg ggggtaccgt ttgagaggta cgctgacgat attgtagtac attgttcacg
     2041 aatgagtgat gcaacaaggc taaagaacag gttatcggag cgcttctcgg aagtcgggct
     2101 ggtcctgaat gcagggaaaa cgaatatcgc ctacattgac acgtttaaaa ggagaaacgt
     2161 cgcaacgagt ttcacctttc tcggatatga cttcaaagtg cgtacgctga agaatttcaa
     2221 aggcgaacgg taccgaaaat gcatgccggg tgcgtcaaat gcagcaatgc gcaaaataac
     2281 agaaacaatc aagaagtggc gtatacatcg ctcaacagct gagagtttgc tggattttgc
     2341 gaggcgctac aatacgatag tgagaggctg gatcgagtac tacggaaagt tctggtccag
     2401 aaatttcaac tatcgactgt ggagtgcaat gcagtcacgt ctgctcaagt ggatgcagtc
     2461 taaatacaga ctttcgaacc ggaaggctca gcgaaagctg acgctggtaa ggaaggagta
     2521 tccgaagcta tttgttcact ggtatttact acgtgcatca aatgagtgat caagagccgt
     2581 atgacgcgag agtgtcacgt acggttccgt gagcagccgg ggagtgaaat tctctccggc
     2641 tgactcgact cgcgggttcc gacagcgatg acgaacatgc ggcggtgttg tactcgctga
     2701 tcggcacatg ccgtctgaac aatgtggaac cagaaaatgg ctgcgttacg tcattgagca
     2761 tatccaggac tggccggcaa accgggtacg tgatctgttg ccctagaaag ttgatctgag
     2821 ctctcagtaa atatcaatac ggttctgacg agccgcttac ttttggcgta attctgctgc
     2881 gcctttactg aacacaatgc cgtattcagc aagaagtcca cgaatgtgat ttatcagttg
     2941 tgtcctttcc attgaaacca accgacgcat attcaaaaaa gtttggacgt cctgttgctc
     3001 cagagttttt attggtacgg gtcgtatagt tccatgttgg caggcctcag ctattgcctg
     3061 tgcgtcgtta taatcatttt tctggcaccg ctgataagct ttaacatgtt gtgccggaag
     3121 caatattaca tcatggtcga gtttcctgat ttcccgggcc cagtaatgag atgccccgcc
     3181 agcctccatc gcaacagtca ttttaggctg ttgcaccagc cagttaagca gttgattgcg
     3241 agaaagtttg gtctttcctg cgggtttgcc atgagcattg atactgaaaa gataaaagtt
     3301 cgttttagcc agatcaatgc cggtgatttt ctgttcagac atggtatccc gcttactttt
     3361 gtggttaaag tttatccatc atggcgcaat tgaaggctgc cgtgaaagag ggggaagact
     3421 atctcatcgg ttactgttaa acggctaaga gaaactatct ttttcaagca aagaagatac
     3481 ggtgaaacag gcgatttcac cgtgaaatac tgttcaaaaa aaacgctaat aaaagcaaaa
     3541 tttcttttaa aacagccaat taaaaactgg tgagttgctg gcgaaaaact tgaattttca
     3601 cagcgaattc agattatctt ttagtacaag agcaatcata cgatctaact tatcctgaac
     3661 ttccaacggt aatctgccga attcatatga gaacgtacgt ccctttactc gttttcgagc
     3721 aaatttatct ttactatcaa atttccataa caaagttaca accgctttat cctttactcc
     3781 tgaatcaagc aatgaagaca tttccttggt tataagccgc ataatactgt ttttaacctc
     3841 gtcttcagaa agattgtcat cagcagtaat aagctcaata ctcggcgaga tattcttcac
     3901 aacttcatca atagatatat cacccagcct cagtcgttca cttaattcag ccagttgcct
     3961 gtaatcggcg tatgtcagct cagactgaac aggaaaaaga gacacaaaat ctttaggtac
     4021 ggacgctgct tggagggcac gagtcacctt agctgccgac aatccctctc tttctgcaat
     4081 ttgcttctgg ttcataccgg cctctttgag acgtactaaa cgcaacccaa tttcacgaat
     4141 attatgttca agtgacgtct gcaaatcttt cgccagatgc tgagcttcag aaacagtaag
     4201 ctcatcatcc gttacaagta cacgtaatcc aaccttgcat agcaaagctg ctgcccgacg
     4261 acgggaacca tccaaaatct caataagatc gccagtccga atcccaatac atggatagaa
     4321 ttgttgtaaa cgtatagttc tggtgatgtc ttttagtgag tccggcgtca gggctgtctg
     4381 ttcgcggcca ttcacctctt caaccacaaa agtttgagtg tccactttgt catgatcaac
     4441 cttgatctca gtaaattttg cctttcggcc agtattgagg gtaaatacat gaactggagc
     4501 cgatatttct tcagtattat tcaggatatt tgtattcagc gtacggccaa tagtcggtcg
     4561 gtgctttctg ttttccatca gtagttggcc cttataaatt caatacgatc aaaaactgct
     4621 ttagcgaaat cttctgctgc catacgtgca tttttcaaag cttcaccact gccaacataa
     4681 gtaacaggat tcgcagatat cacggtatca aacgactcgc cgcttctctc aaaaccatca
     4741 agacgtggca ttgaaacatc aagcatgtca ccaccgaaaa tctcttttgt caggctatgg
     4801 cagtatttat gatctgactt attcgccagt ttcgacatga agccaatatt tgcctgcaaa
     4861 cggcatgaac atccatcctg ttcaataatc tgtacaagtt caggtaaacg agccaaatat
     4921 ttaagcgtgg aatggaaatc aacttgtgct ggcggcaccg gtgtaaacat gatatctgcg
     4981 gcagcaatgg catttttcaa aaatgcgtcc agatgcgggc ctgtatcaat aaggatgaag
     5041 tcgaaatcat gcttcagctt atcaataata ttctcacgca atatcgcgtg tttgttttgt
     5101 cctaatagat gctcctcgca cagagtatcc cagtttgatg caataaaggc gtcgtcaata
     5161 gaagccggca taacataaac gccagggata actgacggaa cgataaaatc ttccagtaat
     5221 tcctcacgac tcacgttctg caacatagcc tgcggtgcgg ttgtatcaac cagccccacc
     5281 gcatgcaagt agttcagaaa catagtggct gatgactggg gatctaaatc aaggagcaga
     5341 atacgtaaat cttctgacag caggtgagga tgagcacgca gggcgtgagc tacggaaaca
     5401 ctactcacgg ttttagacac accgcctttt agactaccga tgaatatgga atatgcctca
     5461 ctataacgat cgcgatattt aggaatgcca cgatgtgcat agatatcgat aatgttctgg
     5521 atggttaatg catattttgt cgcggttcct gctgggcgtt tttcaaactg atagccctga
     5581 gcctccatct caccgactgc accatccacg cttgcgcgcg ttaattttgg aagcttagct
     5641 acagcagact ttgaatagac ttgataaaaa acatcagcct gaaactcctg cttttgctcc
     5701 tgaatctgtt ttgttagggc gagcaacatt ttatctgcac gctgcgcaac cttacttaac
     5761 tgctcaaaac tagtcattcc ccctccgtgc tatgtaggtt tttaacttat tacaaaaaaa
     5821 actgcattag cagggtaatg catgcaagtg catattgtac atgcttatat taaaaatgcg
     5881 cgcattgctg atttattaat catcggaatg aatattactg taagatagta tgcctttccg
     5941 ggagaaagac gctgatttcc cccgaataat ccagttcacc accacactcc ggacaacagc
     6001 tttcttcagg ctccagatgg tgtatttcgc gggaaacatg ttccggtaac ggacggcgat
     6061 gacgtgactg tcgcaacagg cgggggtagt gcttccaatc agtagaacat gtgcttttca
     6121 atgaaggagc cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta
     6181 cgtgccagcc gcttgatgcg ggtgcggagg gtccggttgt tacgttcaat acgttgcgtg
     6241 aagattttgc cggtcaggtg tttctccttc ggcacttctc tggcgtagct gccccagtca
     6301 tcgctggtca ccatgccgat acagaacgga gtcaacaggg ctaacagttc ccgacaggtc
     6361 tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt gttgtacgca
     6421 taccagagcc agtgttgtgt caacgacgga tgaaaagtga tccacttata tctccaccaa
     6481 cggcccaata ttgatccacc gttttactca ggattagctt ctgctataac cccggccttt
     6541 cgtttctgtc tgagtcgata gctttctcct ttgatttgaa cgacatgtga gtggtgtaag
     6601 atacggccca gcatcgctga ggtcagtgct gcatcaccgg cgaacgtttg atcccactgc
     6661 ccgaacggta gattggatgt caggatcatt gcgctctttt cataacgttt agcgatgacc
     6721 tggaagaaca gttttgcttc tttctgactg aacggcagat agcctatttc atcaatgatg
     6781 agcaggcggg gggccattac tccacgctga agcgtcgttt tataacggcc ctgacgttgt
     6841 gccgtagata actgaagtaa cagatctgct gctgttgtga agcgaacttg gatacctgca
     6901 cggactgctt catagcccat cgcgattgcc agatgggttt tccccacacc tgatggcccc
     6961 agtaatacga tattttcatt acgttctatg aagctgagtg agcgtaacga ctggagttgc
     7021 ttctgcggtg ctccggtggc gaatgtgaag tcatactctt cgaacgtttt caccgccggg
     7081 aaggctgcca ttcgggtata catcgcctgt ttacgttgat gacgtgccag tttttcttca
     7141 tgaagcagat gctccaggaa gtccatataa ctccattcct ggtctactgc ctgttgtgac
     7201 agcgcaggcg ctgcg
//
LOCUS       DAJVOY010000187         7203 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVOY010000187 DAJVOY010000000
VERSION     DAJVOY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.187"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(235..789)
                     /locus_tag="OQL90_003542"
                     /pseudo
     CDS             complement(235..789)
                     /locus_tag="OQL90_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            932..1054
                     /locus_tag="OQL90_003543"
                     /pseudo
     CDS             932..1054
                     /locus_tag="OQL90_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1316..1882)
                     /gene="ipgB2"
                     /locus_tag="OQL90_003544"
     CDS             complement(1316..1882)
                     /gene="ipgB2"
                     /locus_tag="OQL90_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCS1370188.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2164..2478)
                     /locus_tag="OQL90_003545"
     CDS             complement(2164..2478)
                     /locus_tag="OQL90_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370189.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2556..2666)
                     /locus_tag="OQL90_003546"
                     /pseudo
     CDS             complement(2556..2666)
                     /locus_tag="OQL90_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="OQL90_003547"
     CDS             complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="OQL90_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCS1370190.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGVPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4038..4175)
                     /locus_tag="OQL90_003548"
                     /pseudo
     CDS             complement(4038..4175)
                     /locus_tag="OQL90_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4257..4555)
                     /locus_tag="OQL90_003549"
                     /pseudo
     CDS             complement(4257..4555)
                     /locus_tag="OQL90_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5144..5303)
                     /locus_tag="OQL90_003550"
                     /pseudo
     CDS             complement(5144..5303)
                     /locus_tag="OQL90_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5307..6461)
                     /locus_tag="OQL90_003551"
                     /pseudo
     CDS             complement(5307..6461)
                     /locus_tag="OQL90_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="OQL90_003552"
     CDS             complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="OQL90_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1370191.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6828..>7203)
                     /locus_tag="OQL90_003553"
     CDS             complement(6828..>7203)
                     /locus_tag="OQL90_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1370192.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
BASE COUNT         1855 a         1678 c         1560 g         2110 t
ORIGIN      
        1 aaaccttctc atgtttagag ttttttatta tgtttatcat aatgatatga cgatctgagt
       61 ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac agtgcctgtg gataactttg
      121 cgtaaatccg gaggacagat cactctgaag caagacaaca gcatctgaaa acaatatgct
      181 cacgtttcaa ccacagggcg taagtgatcc tacccacgta atatggacac aggcctaagc
      241 gaggttctgg ttttcaaatt gttccggact gaggccgcca caccaactgt gccgtcgcca
      301 gcgattgtaa tcacattcga tataattaaa cactgttgcc cgcattattt cccggctgat
      361 aaagtgttct ccgtggagac attccacttt cagcgaatga aagaagcttt ccacgcaggc
      421 attatcgtag cagcagccct ttgcgctcat actgccacgc agattatgcc acttcagcag
      481 cgcctgataa tctgctgaac agtactgact tcctcggtcc gagtgaacga taacgttccg
      541 gggtctctta cgccgccaca gcgccatctg cagggcatcg caggccagtt gcgccgtcag
      601 gcgtggtgac atggaccagc caataacggc acgtgaccac aggtcaatga ccacagccag
      661 atacagccag ccttcatctg tacgtaagta cgtgatgtct cccgcccact tctggttcgg
      721 accactggcg taaaaatcct gctccagcag attttctgac acaggcaggc catgtgcgcg
      781 gtaactgacc gtattgctgg gtaagatcaa tttgctcact ggtatttact acgtgcatcg
      841 aatgagtgat caagagccgt atgacgcgag agtgtcacgt acggttccgt gagcagccgg
      901 agagtgaaac tctctccggc tgactcgact cgcaggttcc gacagcggtg acgaacatgc
      961 agcggtgttg ttctcgctga tcggcacatg ccgtctgaac aatgtggagc cagaaaaatg
     1021 gctgcgttac gttattgaac atatccagga ctgaccggca aaccgggtac gcgatctgtt
     1081 gccttggaaa gttgatctga cttctcagta aatatcaata tgattctgac gagccgctta
     1141 cctttgagca gagaaagggg agagggatcc cacatccatg agctaggaaa atacaaaagc
     1201 tggctataca acaattcaat atattaggac ttacttgcga ggagaaatat agcaccgctc
     1261 aggcaattcc tgggtattta cttttcttag tttctttact cgggatgttc tgtcatcaga
     1321 aaggcgattc taaatttgta atataatcac tcatattccc acgtaatttc tttagatcaa
     1381 tatattgcac atttttctca aaaaactgat cggaaataag atttgcaaca ttgcttgttg
     1441 tatcattttt aacgcttcta ttcatccctt cacaaagttc gtccattttc tttttgaaat
     1501 aactgcttcc tgatacaaga tgatttatgg aactttgtgc agcattgatg tctagagaaa
     1561 tgtgataatc tttctcaacc tgtttaaata tctgtttacg gagattatcg ccgatttttc
     1621 ggctgtgttc tgcacaaaac ttatcaatac attggtttac cacccgggat acgtagactt
     1681 ttttttcatc atttttccaa tctgtaatat tttcaccgat ttgttctgaa acagagctca
     1741 aaatactagt atttattttg gttgatgata tttttactat ccttttcccc attgtacagc
     1801 ggataatctc atcaaccttt cctgaaaaat atcttttgtg agataaactg attccaaaat
     1861 tattaaaaga tgttccaagc attataatca ctttgtcatg taaaatagta acattgtcat
     1921 tattttagct gattcaaaca attctgtaaa tgctttgagt tctttactcc cttggctcaa
     1981 tgatggaccg tctggaatcc cagagactaa actctctgag aaagagagta tgaaaaaact
     2041 tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat aaccggcatc attcataatg
     2101 atgccctgtt agttgtagtt atgaagattt acgtcattaa aattgctgtt cgtagtgtca
     2161 gtcctatggt ctggcatcag ctgaggatcg tagccgatac atcactggct tcacgacatt
     2221 cgtgttgagg ctattcatga aaactccacg ctgaaaacgc ctttttgtat aaacggtcac
     2281 ggtatgcccg gagccactgt tgctgatgaa ttcgataaaa ccttggcgtt tcttgaagct
     2341 atcgttaatg cggataatga aacaacgatt ggtgagattc gctcttttgc cgacaccctg
     2401 ggcgctgtcc ggttcaatcg caacaaaatc aaccggcagc tgagcaaacc caaccttgcc
     2461 tctctggcgc tggagcatga agtgatttag ctgggacgta gccactgaca gcttctataa
     2521 aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg gttttcaaat
     2581 tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta atcgcacttg
     2641 atataattaa acactgtata tttcatttgt ggagtctcca gtctgagggg gctttatgca
     2701 ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc ctgtatttcc
     2761 cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta catttattgt
     2821 gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg ttctatgggt
     2881 agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag atcacacaat
     2941 tcggggagag ctccttctaa gtgcaattct ttgcagctat ttagcacact taaaacaact
     3001 cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta agtctgtctg
     3061 ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc atcatagcct
     3121 cgatattccc tttccctgcg gccagaaata atcctggtac cccctcatta tctcttacat
     3181 ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa tctccataag
     3241 cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat tccccagcat
     3301 tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct gccaacagcg
     3361 attcaccgta tttacgcgct gcatcagcat ttccatgcca aatcaatgaa aacaaattat
     3421 ggcggttata ctccttatta ttctttgtcg gaatggatat gctctgtatt ttggccaatt
     3481 ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg gcgtgggata
     3541 tagcggtatt tgtcacgtta atgatattac gtttcattcc tttattttca ttagcagtat
     3601 tgctgcctat tattaggtgc atatttttgt tgtttgctgg atgtaatcca tagttattta
     3661 ttgacatttt tgttcatcct ttttgctaaa aaaaacaata acctattttt gtaatgtaat
     3721 agatattagt ctattgctaa tctaaaaaaa ccattgccac attgaatggc tatattcacc
     3781 aataaattct ttcgatttcg tatattgaca tatcgtagat atatcttatc cctaagattc
     3841 aataatcgag tccgtttacc attctgtgca agcgccttca ctcagaagtg accgtcaagg
     3901 cgatcaccaa acttgataat aaagcagctc attgccatac gccagtcctt aaggggcgtt
     3961 gtccatttct gtgatgctgc ctagatttaa ctgcctgctc cggcttatct acatctaaat
     4021 atgtgagcct aataccgcta cactttggcc agactatgtt tttctccggg aagtgggcta
     4081 accgtctcca taaagtgacg aacttttttc aacagtggcc atattgaacg gcactgatga
     4141 ttacgggtta ttgtctcatg aagagccggc cacatcactg acagcagtga cttcaggcct
     4201 tcagcaccag acagcctgaa ccagttaatc catcgcccga cagatttagt aatctagggc
     4261 tggtttttct tcagctgttg cggttcgaaa gtgccattac gatcacgcag agtacggatt
     4321 tccagaggtt catctccagt agtgataatc ttcgttaagc aaccattgcg ggaattgctg
     4381 ccagatctaa gctgattttt ctcgcaccca agatgatgag tcatattagc attgagtacg
     4441 gtttcatttt cagcatccaa tcaaactgac tgagatcttc aggatttttg agacttttgg
     4501 ctagttcatt agctaaagcc cgcaacttat tagatgtcaa ctgtttttca ttcataaatt
     4561 caccttcatt tgatatcaaa ttaaatagtt caacatcaga gaattataca aacttatgta
     4621 caggctccta tgatcttcga atcctgaaga ttatagtgaa aataagtttt cacgttgaaa
     4681 tcccacatag ttagtatatg ataaataatc atggatgacc tgattaacct cttggggtag
     4741 tgcttttata gaagaataaa tattctacta atttaataca tcacctaaaa tagtattgtt
     4801 ttttatggtt ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat attccagtgc
     4861 cagatcttac ttataataaa ggggcttggc agtttggata aactattgtt cttattccac
     4921 taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatactga aatccccgga
     4981 agtattcaat caaacaatta caccataaaa ggaagagggg gccctaatta agttgtcaag
     5041 catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac atataaaacg
     5101 tctttattga tcctacccac ataatatgga cacagtcgta agtgaggctc tggttttcaa
     5161 attgttccgg actgagacca ccacaccaac ggtgccgtcg ccagcgattg taatcgcact
     5221 cgatataact aaacactgtt gcccgcacta tttcccgatg gataatagtg ttctccgtgg
     5281 agacattcca ttttcagcga gtgaattttc atctgttgct gtatccagtc atacagtgac
     5341 tgcatcagtg gcgcggctct ggcttttctt gccgccagac gctgttctgc tgaacatccc
     5401 cggacttctg cctcgatggc atacagttca ccgatacgca gcagggcttc cgttattctg
     5461 ccggattcgt acaacgcccg gtaaccaccg taagcatcgg cctgaagcac tccgctgtaa
     5521 ccggccagat gattctgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
     5581 gccggaggca tttccgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5641 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5701 ttaccgggca tcagcacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     5761 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     5821 atttctgact ggcggtataa cggcagatag cctgcatatt ttccagcgac aacatgggcc
     5881 agaagccccg ctccggcata actgcgtgca atgggttttg aaggcaccgg tgcctgcacg
     5941 atatggtcac accggcaaca ggccagtttc ggacgttgtg tttcgataac cttaaaggcg
     6001 ctgctgataa gctccagctg ctctgacaca tcacatccca gagaactgag ttcgccacca
     6061 caggccgggc agcattcctc ttccggacgg atgacccggg tttcacgggg aagtgaggca
     6121 ggtaacggtt tacgggctga agactggcgc aggggagatg gcagtaccgg gtcatattgc
     6181 tcacccagcg tttccgccat ttcttcctga agtgcgctga ttcgctcctg tgcttccagt
     6241 atctgccgtt cggtttttgc gcgaagtttt tctgagcttt aaccgaactg catgcgctgc
     6301 agtttcgcaa ccagcgcctt cagccggttg atttcggaag cataagccgc cacccgctgt
     6361 gagagcaggc ggttgtattt cgccatctgg cggatggtgt cctgttgcgt ctgcaacagt
     6421 gcccgcaggc gggcattctc atgagcaagt gaggtgtcca tatcctcact ttacaacggg
     6481 ttatatgcgg atgccagcgc gttccgttcg tttcgggtgt ttccagtcga tgccctcaag
     6541 aagcatggat aactgggccg gagtaaggtg caccttgccg tcacgggtga ccggccagac
     6601 gaagcggccc cgctccaggc gtctggtaaa gaggcacagt ccgtcactgt cagcccacaa
     6661 cacttttatc tggtcacccc ggcgtccgcg gaagatgaac agatgcccgg agaacgggtc
     6721 atccttcagg acgttctgaa cttttgaggc caggccgtta aagccatttc gcatatcggt
     6781 gatacctgca accagccaga tacgcgaacc tgcagggaaa gatatcatca gtggctgctc
     6841 ccttttattt cgcggataag tatctgtaat aacgccggcg tcagtttacc tttaagcctg
     6901 agagttccgg ccggcagaac cagctcacag cacagactgt cggacggtgt atttatctgc
     6961 tctggttcct gtgccggagc cgggatttta ttatccggct ccggcgttaa cgtcacggga
     7021 agcagtgcag gcatattttt tccggaaggc agcaggccac ctttccggta ttgatggcgc
     7081 cagttgaaga gcaggttatc gttgattccg ttttcacggg cgatctgcgc cacacaggct
     7141 ccgggctgca gtgactgctc cactaaggcg attttaaatt cataaggaaa attaggccgc
     7201 cgg
//
LOCUS       DAJVOY010000188         7192 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVOY010000188 DAJVOY010000000
VERSION     DAJVOY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.188"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            168..275
                     /locus_tag="OQL90_003554"
     CDS             168..275
                     /locus_tag="OQL90_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370193.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(328..1086)
                     /locus_tag="OQL90_003555"
     CDS             complement(328..1086)
                     /locus_tag="OQL90_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1370194.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1373..2302
                     /gene="pfkB"
                     /locus_tag="OQL90_003556"
     CDS             1373..2302
                     /gene="pfkB"
                     /locus_tag="OQL90_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1370195.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2403..2693
                     /gene="ghoS"
                     /locus_tag="OQL90_003557"
     CDS             2403..2693
                     /gene="ghoS"
                     /locus_tag="OQL90_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1370196.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2799..3659
                     /locus_tag="OQL90_003558"
     CDS             2799..3659
                     /locus_tag="OQL90_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1370197.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3700..4236)
                     /locus_tag="OQL90_003559"
     CDS             complement(3700..4236)
                     /locus_tag="OQL90_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1370198.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4383..5051
                     /gene="hxpB"
                     /locus_tag="OQL90_003560"
     CDS             4383..5051
                     /gene="hxpB"
                     /locus_tag="OQL90_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1370199.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5214..5792
                     /locus_tag="OQL90_003561"
     CDS             5214..5792
                     /locus_tag="OQL90_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1370200.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKH"
     gene            5937..>7192
                     /gene="tcyP"
                     /locus_tag="OQL90_003562"
     CDS             5937..>7192
                     /gene="tcyP"
                     /locus_tag="OQL90_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCS1370201.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEP"
BASE COUNT         1807 a         1750 c         1773 g         1862 t
ORIGIN      
        1 tattttcgat atatatgttg atgcataata ttattttttc ataaaacgca gaaacagtga
       61 ccaggtgtac aacgccaccc aacggctgta ataacaaaaa atcccttgat gcccgtccct
      121 tttgttacac tccgttatca cgcacaagag atatgcagga cactggaatg ccgactaaac
      181 gctttgataa aaaacactgg aagatggtgg tagtgctact ggcaatctgt ggcgctatgt
      241 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
      301 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
      361 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtaa cgttgtaagt
      421 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
      481 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
      541 ccatgcataa gccccggagg catagcccag cggctgagtt tcactggcat tgtcggtata
      601 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
      661 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
      721 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
      781 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
      841 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
      901 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
      961 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1021 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     1081 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     1141 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     1201 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     1261 tatatatatt tatctgcaaa attttaaata aagctccaat aaatcatatt gttaatttct
     1321 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     1381 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     1441 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     1501 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     1561 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     1621 agaagccaaa gactggaccc ggcagaattt acatgtacat gtggaagcaa gcggtgagca
     1681 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     1741 gcaagttctg gaaattgaat ccggggccat cctagtcata agcggaagcc tgccgccagg
     1801 tgtgaagctg gaaaaattaa cccaactgat ttccgcttcg caaaaacaag ggatccgctg
     1861 catcgtcgac agttctggcg aagcgttaag tgcagcactg gcaattggta acatcgagtt
     1921 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccgga
     1981 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     2041 ttcccttggt ccacaaggag cgctgggtgt tgatagtgaa aactgcattc aggtggtgcc
     2101 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     2161 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     2221 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     2281 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     2341 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     2401 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat
     2461 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     2521 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     2581 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     2641 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     2701 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     2761 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     2821 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     2881 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     2941 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3001 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     3061 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     3121 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     3181 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     3241 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     3301 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     3361 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     3421 ttttcgaccc tgcctgctac tggggtgacc gagagtgcga cctggcgatg ttaccgctgc
     3481 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     3541 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaaggttat
     3601 ttggcggtca gcatttggtt attgctcagc agtcactgga tagattatta gcagcatgat
     3661 atgggttgaa gataatggcc gctctgtgcg gccttttgat taaataaacc caagcagaga
     3721 aaagaacaca taacctgcag caatacaaat aaccggcagg atatacagtg agaaaaactg
     3781 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     3841 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     3901 cgcctggcga ctcattcggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     3961 gaaaatcacc cagaacataa cgttcacgcc accattaaaa ttgggcgtcg gggagttata
     4021 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagtaaa
     4081 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     4141 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     4201 caaaacagca atgtgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     4261 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     4321 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     4381 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     4441 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtggata
     4501 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     4561 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     4621 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     4681 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     4741 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     4801 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     4861 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     4921 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     4981 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     5041 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     5101 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     5161 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     5221 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     5281 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     5341 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     5401 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     5461 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     5521 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     5581 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatccta gttccccaaa
     5641 agggcaacca actggaacgt tttatctgta tggcattatt tgtctggtcg gtatggatgc
     5701 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaataatc aataccttgc
     5761 agatccagtt tcatcggctt attaagcatt aggttgaata ctaaaaagac aaaaatcacc
     5821 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     5881 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     5941 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6001 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     6061 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     6121 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     6181 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     6241 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     6301 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     6361 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatctga
     6421 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     6481 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     6541 tgaaactgct gaaggatgat gcgccgaaag gtgaacgcgt cttagccgct atcgataccc
     6601 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     6661 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     6721 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgcagtgcat ggcattctgc
     6781 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     6841 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     6901 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     6961 gtcagaacgg ctgcgccggt ttgtatccgg caatgctggc ggtgatggtt gcgcctacgg
     7021 ttggcattaa cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta
     7081 gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc
     7141 ctgcgatggg cctgccagta accctggtgg cgctgttaat ctccgttgaa cc
//
LOCUS       DAJVOY010000189         7173 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVOY010000189 DAJVOY010000000
VERSION     DAJVOY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.189"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(784..1314)
                     /locus_tag="OQL90_003563"
                     /pseudo
     CDS             complement(784..1314)
                     /locus_tag="OQL90_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1657..2307)
                     /gene="zinT"
                     /locus_tag="OQL90_003564"
     CDS             complement(1657..2307)
                     /gene="zinT"
                     /locus_tag="OQL90_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1370202.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2564..3199)
                     /gene="msrQ"
                     /locus_tag="OQL90_003565"
     CDS             complement(2564..3199)
                     /gene="msrQ"
                     /locus_tag="OQL90_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1370203.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3200..4204)
                     /gene="msrP"
                     /locus_tag="OQL90_003566"
     CDS             complement(3200..4204)
                     /gene="msrP"
                     /locus_tag="OQL90_003566"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCS1370204.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4313..4726)
                     /gene="hiuH"
                     /locus_tag="OQL90_003567"
     CDS             complement(4313..4726)
                     /gene="hiuH"
                     /locus_tag="OQL90_003567"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1370205.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4859..5530
                     /gene="hprR"
                     /locus_tag="OQL90_003568"
     CDS             4859..5530
                     /gene="hprR"
                     /locus_tag="OQL90_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1370206.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5530..6888
                     /gene="hprS"
                     /locus_tag="OQL90_003569"
     CDS             5530..6888
                     /gene="hprS"
                     /locus_tag="OQL90_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1370207.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASLGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(6996..>7173)
                     /locus_tag="OQL90_003570"
     CDS             complement(6996..>7173)
                     /locus_tag="OQL90_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS1370208.1"
                     /translation="HLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANAL
                     GKLAAQEMLAAYAG"
BASE COUNT         2013 a         1480 c         1621 g         2059 t
ORIGIN      
        1 tctgaatgac acctgaaaac aggtggtgga ctcaagaagg cttggaatat tcttctttaa
       61 tgttatgtaa ttcattgatt tttcgtgtac gattttaaga cattttttcc aagaaaaatt
      121 tttaactctt tgatttttcg actctgtatc atcggtctcg aaaaccggag taggggcaac
      181 tctaccgggg gggcaaatcc ccctctctcc gccactttat caatgactta tctcccgact
      241 tcccgcctta cttttcctaa acagaacaat cgtagaatat tcttgaaggg ttagatcgtc
      301 actgttttct gttcgatact gtgacattca gcacttgatt cgctatggat ctgacaggaa
      361 gctttcgagc gaaaatgtgc agttattcag tcgttttctt atcgctcacc attattcttt
      421 tagacattca tcctatgaaa ctgccgcaaa gttggtggtg ggaactgaag ttgcgtagag
      481 aaggggtcaa taattgagtc aaaaaaggaa cgcggccgtc agattaaagc cgcgggacaa
      541 agtccataat ttccaagagt tgttgaattt cccagctttt ttgaaagcct ttgtatcata
      601 ctaacaagta aaaaaggttt aaataccaaa attaacctgt atttttgata agtcttacca
      661 ttgtacgatt tacacagttt ctgctcctcc ttgttgatag tggaaagggt gcattgtgca
      721 cttgcgtaaa catttatgac gcgttgatgt ccatggttca catttccttg tcaggggatc
      781 gacttatttg cggcgaggca tcattcgtaa cagagtatta tctttctgaa tgtagtgatg
      841 aaagagtgct gcgccagcgt gagctgcgat taaaaagtaa cctatatttg cccaggtttc
      901 gtgaatattt tatatcagtg ctttaatttc gctgtttggg gtaacaaagg gagacacatt
      961 gaaaccaagg aaactccacg attttccact gtaagccatc aaagcaatcc ccagcagagg
     1021 aagtgcaagg aaggttatat aaagcatgat gtgcattagc gaagccgctt tcatttgcca
     1081 ggcaggtggc ggtggcacaa tagaaggatc actataacgg tgttttataa tcaggcgtga
     1141 aaacattaac acccaaacga atatacccgc attgtaatgt gtttctcgca tcagcagata
     1201 agtactacta cctttaggaa accagccacg gagttccatc gcggcatagg ttattgcaac
     1261 gaaaagcagc gttatccaat gtaaataaat ttgagttttt gagaatcggt tcatttttgt
     1321 agctcctgta atttttacag tcaagctatc ccacgaacct taaaaattcc ttatacagat
     1381 gtggttttaa gaatttgtta tttaaaaaat tgatagtgca cattgtaacc agccaatata
     1441 agcaatggcg tgagcacagg tatctttctc tgttggccgt attgtgtatg gaatccgtta
     1501 ttggttaact caatgcgggt tagcttatta atatacagac acgtcatagt gatgagcgtg
     1561 tctgtagcta gcttgcgttc agtggcgttt gtggatggac cagtggcagt tatgatgact
     1621 tactaaagcg gcatcgaggc gttatcatga gaatactcaa tgagacatca tttcctcgac
     1681 cacttcttcg ctactcaact gatatggata atacgttggc cagttttcca tttcatttaa
     1741 taaagattgc tgtgaatcat tacccataaa aatgtgaaaa tgagatgatt ttcgtggtgc
     1801 aattatatga tcgctaaatt gtatatattt aggggctttg ctttcaggat ctttacattc
     1861 gaataagtaa cgaacgcctt tcttgcctga tttataggtg agtattttgt atccatcgta
     1921 atcatattta caggatgttg tttcattatt tctatggaat tcaacaatgc cgtcctcaat
     1981 gccaatcatc tcgatatctg ttgcataacc tttgtgataa taatctttaa tttcagcaaa
     2041 tgttttagtt ttatctgcat ccgctttctt ctgaaagacg gggtcaagtt tgccactttg
     2101 cagtaaagga taaacggatt gccagacccc atcccagtca ctgagcgttc ggttttgtac
     2161 attggcatca tcaaaaacac cattagcagc tttttgttcg acctctgtta agggtttgcc
     2221 gtgtgagtga tgaccatgcg aaaaggcagg agcgctaaca ataaagacac ctaacgcaac
     2281 agccagtttg tgaagacgaa tcgccaaaac agtttcctcc agagtcattg tatatgatgt
     2341 gtaatgttat attgtaacat atggcaaaat gcaacaactc ttaggagatg agagcatagt
     2401 tttctcaggt gtacttatct atcttattga aataaaatga attatattga taatttgagg
     2461 ttgatgacaa acgaaacata atctgatgtg atctgcgtag gccggatagg gcgctcgcgc
     2521 cgcatccggc agggataacg cgtactttgt cggcaatatg aagttataca gataatttgt
     2581 tgtgaacctg cttgcgtaac cggttaaaaa gggaacgcaa cttcttatac cgtaaggcta
     2641 aaagcagtac agccagccca gcgtagatga gcggctgcgg tgaaataatc ttcactgacc
     2701 acagataatg tattggggcc aggatcgcga caagatagac gaaattatgc aactgttgcc
     2761 agtgcttgcc gagttttcgc tgcatcgcct gggttgaagt aaacgctaaa gcaagcaaaa
     2821 taacccagct gataattcct aacgttaaat aaggccgggt aattaactcc ttgcctaata
     2881 acgccagatt attcacgccc agctccagca atgcgtaact ggttaaatgc agtgtcgccc
     2941 aggcaaagca ccataagcct aacaggcggc gagtgcgtat caataacggc tgttttgcgt
     3001 agcgtgccag aggtgtgatt aacaaggtcg ccagcaaaaa tttcagtgca gtgcgaccgg
     3061 taaaatgctg aatatctttc accggatcgg cacccagtcc accgtgattg atcgcccaga
     3121 ccagccagag aaacggcaac aatccggcaa gatgcaggct aactttcagc catgtcacct
     3181 gttttgctgt cagacgcatt cagaaattct cccgcaaatc caggccacga tacagcgatg
     3241 ccacctggtc ggcgtaacca ttaaacagta gcgttggctg gcgctgaaca tcgaggatgc
     3301 cgcctgaacc aataaatcgt tcggtagcct gtgaccagcg cgggtgatca acatgcggat
     3361 taacgttggc gtaaaaaccg tattcgtcag gcgctgccag attccaggtg gttggcggac
     3421 gctcgcgggt cagcttaata ctgactatcg atttaatccc tttaaagcca tatttccacg
     3481 gcacaatcag tcgtaccggc gcgccatttt gtggcggtaa cgccttgcca taaacaccca
     3541 cggtcatcag tgtgagcgga tgcattgctt cgtcgagacg caatccttcg acataaggat
     3601 atttcagccc gccgccgata aagcggtcct gctggcctgg catttgttcc ggtgcataaa
     3661 ttgtttcgaa agcgacatac ttcgcattgc tggtgggttc ggcaagcgcc agcaatttgt
     3721 gcagcggaaa accaatccac ggcaccacca tcgaccatgc ctccacgcag cgcatacgat
     3781 aaatacgctc ttccagcggg aagcgacggg ttaaatcatc gtgatcgagg gtcaatggtt
     3841 ttgccacttc gccgctgatt ttcagtgtcc atggatcggt tttcaggcta ccagcattag
     3901 cggcgggatc ggctttatcc agcccgaatt catagaagtt gttataaccg gagactttat
     3961 ctactggcgt cagtggcagg ttattttgcc aggcggcagg cttgctgaac tccagcggtt
     4021 ttccggcggg ggcgggcggg cgatcgttcc ctttaaacca gctaagcaga tcggcatgcg
     4081 cagcgtgagg caaagaaagt gcagctgcac tgatgcccag tgcttttaac acctgccgac
     4141 gcttcataaa gaataccgac tcggccgtaa catctgattc ttttaaaaat tgattctttt
     4201 tcataagcca tcacactttt ttgtcgttag ccagcaagag ccgtttactg ttaatctgtc
     4261 agggagtgtc ctccctaaca gtagcagaga tctgctttta catatttatg cattaactgc
     4321 cacgataggt tgaataccca tattggctaa gtaataaagg cacatgataa tgctcgttca
     4381 ctttattaat atgaaattca acggggatct cagggaagaa actttcaaga ttttgtttct
     4441 tgaaatagtc cccggtttta aatacgacac ggtaatcgcc cgtagttgca gtttgctcgg
     4501 gccacagtgc tttaattcgt ccatccttat ctgttttggc ggtattaagt tgtaaccagc
     4561 cgttatccgc cttcttttca agagtgactg tcacgtcagc agcgggcttt ccggtttgct
     4621 ggttcaaaat gtgcacgcta agaatgtttt gttgtgcggc ataaaccaaa gaaggtaatg
     4681 aaaatgctgc cgttgctacc gagagtacta aataacgctt taacatgttt atatccttgt
     4741 catgtgaatg agttttgagg atagcgaaaa agcggccagg gtaaccttac agcagcatta
     4801 caattttgta atgaaccagg ctgtttctat aacatatgat ttatggcata ttattttcat
     4861 gaagatttta cttattgaag ataatcaaag gacccaggag tgggtaacgc aggggctttc
     4921 cgaagcgggt tatgtcattg atgccgtttc tgatggcaga gatgggcttt atcttgcgct
     4981 gaaggatgat tatgcattga taattctgga tattatgctt ccgggtatgg atggctggca
     5041 gatcttacaa acgttaagaa cagcaaagca aacccctgtt atttgcctta ctgcaaggga
     5101 ttctgtcgat gacagagtca gagggctgga cagtggagca aatgattatc tggtaaaacc
     5161 tttttcattt tctgagttgc tggcaagggt tcgggcacaa ttaaggcaac atcacgcttt
     5221 gaattcaaca ttagaaatca gtggcttaag aatggactct gtaagtcaaa gtgtgagcag
     5281 ggacaatatc agtattacac tgacgcgcaa ggagtttcag ttactttggc tactggcctc
     5341 cagagctggc gaaattatac ccagaacggt tattgcgagt gaaatttggg gaatcaactt
     5401 tgatagtgat accaatacgg tggatgtcgc cattcgcagg ctccgcgcaa aagttgatga
     5461 tccttttcct gaaaagctaa tcgccacaat ccgggggatg ggctattcat tcgtagcggt
     5521 aaaaaaataa tgaaaagatt gtctataacc gtccgtttaa ccttgctttt tatattgctg
     5581 ctgtctgttg ctggcgctgg aattgtctgg actctctata atggcctggc aagtgagttg
     5641 aaatggcgcg atgatacaac actcattaac cggacagcac agatcaagca gttgttaatt
     5701 gatggggtaa atccagatac gttacctgta tactttaacc ggatgatgga tgttagtcag
     5761 gatatcttga tcattcatgg tgatggcatc aataaaattg ttaaccggac aaatgtcagt
     5821 gatgacatgt taaataacat acctgctagt gagacaatca gcgcagctgg catttacaga
     5881 agcattatta atgatactga gattgatgct ttacgaatta atattgatga agtttcgcca
     5941 tcattaacgg ttactgtggc taaattggct tcagctagac ataacatgct tgaacagtat
     6001 aaaattaata gcattataat ttgcattgtc gccattgtac tttgctcagt attaagtccg
     6061 ctgttaatca gaacgggatt acgagagatc aaaaagttga gtggtgtaac ggaagcgctg
     6121 aattataacg atagccgaga gcctgttgag gttagcgcat taccgagaga actaaaacct
     6181 cttgggcagg cgttgaataa aatgcatcaa gccttagtca aagattttga acgcctaagt
     6241 caatttgctg acgatctcgc tcatgaactt agaacgccca ttaatgcatt actgggtcag
     6301 aatcaggtta cgctcagtca aaccagaagt atcgctgaat atcaaaaaac aattgccggt
     6361 aacattgaag agttggaaaa tatttcgcgg ttaacagaga acatactgtt tcttgcccgg
     6421 gcagataaaa acaatgtttt ggtgaaactg gactcgcttt ctctcaataa ggaagtcgaa
     6481 aatttgttgg attatcttga atatctttca gacgagaaag agatttgctt taaggtcgag
     6541 tgcaatcagc aaatctttgc ggataaaatt ttactgcaac gaatgttatc gaatcttatt
     6601 gttaatgcca ttagatattc accagaaaaa tcgcgtattc atataaccag ttttcttgat
     6661 accaacggct atcttaatat tgatatcgcc agtcttggaa cgaaaataca tgagcctgaa
     6721 aaactcttcc gtagattttg gcggggagat aattcgcgtc attccgtagg tcagggactt
     6781 ggtctttctt tagtcaaagc gattgccgaa ttgcatgggg gaagtgcttc atatcactat
     6841 ctcaataagc ataatgtgtt ccgaattacg ttaccgcaaa gaaattaaca tagggcttca
     6901 gtacgccata aattaaccgt gaaaaaacgc agccacgtcg tgagtactaa cgcggctgcg
     6961 attaattatg cgcttacatt caaacgtaac agggattaac ccgcgtaagc tgccagcatt
     7021 tcctgcgccg ccagtttacc caacgcattc gctgcgaaag gactgtcgcc ggtgagaagt
     7081 ttacggtcct tatgtactcg cccggtgatg tcgtcattaa tgatgttcat gcccattttc
     7141 ttcagctctt cgccgaagta ccaggtgaga tga
//
LOCUS       DAJVOY010000190         7111 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVOY010000190 DAJVOY010000000
VERSION     DAJVOY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.190"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..649
                     /locus_tag="OQL90_003571"
     CDS             <1..649
                     /locus_tag="OQL90_003571"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCS1370209.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            740..1987
                     /gene="tnaB"
                     /locus_tag="OQL90_003572"
     CDS             740..1987
                     /gene="tnaB"
                     /locus_tag="OQL90_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCS1370210.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2119..3294
                     /gene="mdtL"
                     /locus_tag="OQL90_003573"
     CDS             2119..3294
                     /gene="mdtL"
                     /locus_tag="OQL90_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1370211.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3269..4228
                     /gene="yidZ"
                     /locus_tag="OQL90_003574"
     CDS             3269..4228
                     /gene="yidZ"
                     /locus_tag="OQL90_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1370212.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4385..5134
                     /locus_tag="OQL90_003575"
     CDS             4385..5134
                     /locus_tag="OQL90_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1370213.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5156..5722
                     /gene="yieF"
                     /locus_tag="OQL90_003576"
                     /gene_synonym="chrR"
     CDS             5156..5722
                     /gene="yieF"
                     /locus_tag="OQL90_003576"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCS1370214.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5776..>7111)
                     /gene="adeP"
                     /locus_tag="OQL90_003577"
     CDS             complement(5776..>7111)
                     /gene="adeP"
                     /locus_tag="OQL90_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1370215.1"
                     /translation="SQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVYI
                     VFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVVV
                     QAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMGL
                     KNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLGW
                     MLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVTD
                     KAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAVV
                     VGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAVM
                     MPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1689 a         1822 c         1803 g         1797 t
ORIGIN      
        1 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
       61 gggcggcttg ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac
      121 cctttgcgtg gtgcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga
      181 gcgtctggcg gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc
      241 gcaggtacag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
      301 cggtcacgcg gcattcgttg atgccggtaa actgctgccg aatatcccgg cagaccagtt
      361 cccggcacag gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
      421 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
      481 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
      541 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta
      601 cgaaccaaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
      661 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
      721 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
      781 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
      841 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
      901 tcattccggg ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa
      961 caccatcacc aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc
     1021 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga
     1081 aacgatatca atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc
     1141 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt
     1201 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     1261 cgattactcc atcctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta
     1321 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     1381 cctgattatt tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc
     1441 gctgctggcg ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg
     1501 cgaaagcttt aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt
     1561 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc
     1621 tgttgccagt tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     1681 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     1741 tgcgttgttg tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct
     1801 gtgcgccacc atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa
     1861 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     1921 ctttggtata accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     1981 tggctaaatc cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt
     2041 atgtccaaat ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact
     2101 atcaggacgc tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt
     2161 tatcccgccg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     2221 gccagcgaag cgcagttgca tattgcgttc tccgtatatc tggcggggat ggcagctgcg
     2281 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa agccggtcgc catacccggc
     2341 gcggcgctat ttattattgc ctcggtgttc tgttcactgg ctgaaaccag cacgttattt
     2401 cttgcaggcc gatttctaca ggggttgggc gcaggctgtt gttacgtagt ggcgttcgcc
     2461 attttgcgcg acacgctgga tgatcgacgt cgggctaaag tgctgtcatt actcaacggt
     2521 attacctgca tcattccggt gttagcgcca gtgctcggac atctgattat gcttaaattc
     2581 ccgtggcaga gtctgttctg ggcgatggca atgatgggca tcgcggtact gatgttgtct
     2641 ttgtttattt taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa
     2701 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     2761 gtttcggtga tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt
     2821 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     2881 tcattctcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg
     2941 caggtgttat tccttgcagc ggggatcacc cttgccgttt caccttccca tgcggtttct
     3001 ctgtttggta tcacactgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt
     3061 caggcgttag gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
     3121 caggtttgcg gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg
     3181 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc
     3241 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     3301 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     3361 gatgaacgtg accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     3421 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
     3481 ggagcaaaat ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca
     3541 ccagacaccg cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat
     3601 gcttaatgcg ctgtcgaaac ggatctacca acgttacccg caggcgacca tcaaactacg
     3661 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc
     3721 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     3781 ttatgaagtg ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact
     3841 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     3901 acagagcgac acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     3961 tgctatgagc ctgccggaat ttgagcagtc actgtttatg gctgcgcaac ccgacaatct
     4021 gctactggcg accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt
     4081 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac
     4141 cctgctgtgg cataaacgga acagtcataa tccgaagatc gtctggttac gggaaaccat
     4201 taaaaacctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca
     4261 ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct
     4321 gtccatcatt ccagtaaatg attactctta tattcataat ggaccattaa gcatggagcg
     4381 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     4441 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     4501 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     4561 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc
     4621 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     4681 tggcctcgat atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga
     4741 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     4801 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     4861 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     4921 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     4981 cacgcccgcg attgaaaagc tcagtgtctg ggagttagat ggcaaacaca gctggaaaag
     5041 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     5101 gctctctacc gtgaagtctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     5161 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     5221 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     5281 cattgccgac attcccctgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     5341 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     5401 atataactac tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     5461 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     5521 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     5581 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatcggc aaaccggaga
     5641 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     5701 tattcagcga gttaagatct aaataaaaaa cccgccagta atcatgcatg gcgggttttt
     5761 aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa acagcagcgc
     5821 aacgatgatt acgcacgggc taaggtcacg cagacgcccg gtaccaatct tcatcacgca
     5881 gtaggagata aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac
     5941 ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     6001 tgaggtcatc aacacgccaa cgtagatcaa cgcgccagct gcagcgtagc ctggcaccat
     6061 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     6121 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     6181 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     6241 catgcgcggg aatttcccct tctcatccgc cagacctgct ttatcggtca cgccaatcag
     6301 cgtaccggag gagtcaaaca agttgaccaa catgaaagag aaaatcaccc ctgccagccc
     6361 gaggttaaac gacccggcta aatctacatg acccacaact gtcattacgc tcggcggcgc
     6421 agaaacgatg ccattgtagt gcacatcacc cagcatccag cccagcagcg tcgtcaccac
     6481 gatagaaacc agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa tgatgaagaa
     6541 gccgaggata cccagaagta cgctgtgaga agtcagatta ccgatgctca ccagcgtttc
     6601 cgagttagcg acaatcacac ctgcgttttt cagccccatc atgccgataa acaggccgat
     6661 accgctggta atacccacac gcagacttac cggaatgtta gcaatcatcc agtaacgaac
     6721 gcggaaaatg gtcaaaagaa gcagtccgac agcgccccag aagattgcgc ccataccgat
     6781 ctgccacggc agccccattg cctggacaac cacaaaagcg aaaaaggcgt taagccccat
     6841 cgcaggagcc agcgccaccg gaaggtttgc aaacaatccc atcataatgc taccgaaagc
     6901 agcaatcaga caggttgtta cgaagacggc actggtgtcc atgccagcaa cgcccagaat
     6961 ttgtgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     7021 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     7081 gccagaagcc tgggttgtgt gttgttgact c
//
LOCUS       DAJVOY010000191         7016 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVOY010000191 DAJVOY010000000
VERSION     DAJVOY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.191"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..237
                     /locus_tag="OQL90_003578"
     CDS             <1..237
                     /locus_tag="OQL90_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370216.1"
                     /translation="LFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLN
                     KMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(206..1942)
                     /gene="aceK"
                     /locus_tag="OQL90_003579"
     CDS             complement(206..1942)
                     /gene="aceK"
                     /locus_tag="OQL90_003579"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCS1370217.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASELWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2223..3520)
                     /gene="aceA"
                     /locus_tag="OQL90_003580"
                     /pseudo
     CDS             complement(2223..3520)
                     /gene="aceA"
                     /locus_tag="OQL90_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3550..5151)
                     /gene="aceB"
                     /locus_tag="OQL90_003581"
     CDS             complement(3550..5151)
                     /gene="aceB"
                     /locus_tag="OQL90_003581"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCS1370218.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5420..6349)
                     /gene="metA"
                     /locus_tag="OQL90_003582"
     CDS             complement(5420..6349)
                     /gene="metA"
                     /locus_tag="OQL90_003582"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1370219.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6506..6953
                     /locus_tag="OQL90_003583"
                     /pseudo
     CDS             6506..6953
                     /locus_tag="OQL90_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1682 a         1802 c         1824 g         1708 t
ORIGIN      
        1 ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc gccacaaaaa
       61 tactctgcct ttgagttggc ttttgaattt ggtcaccggg ttattgctga attaatcctt
      121 aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg atacattgcg
      181 gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt acgctaaatc
      241 tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg cggttttgta
      301 gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac agcggaccaa
      361 tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg cccggcgaga
      421 cgctgtacca cagttcgctg gcaagttcgt cttccggata gcgcggcggc gggatgtcgc
      481 ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc acacgcccgt
      541 gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta gcagcggcaa
      601 gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga ccttccactt
      661 gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg cgaattacaa
      721 tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat tccattaatg
      781 ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg tccgccattc
      841 ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga gcggcagaca
      901 tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca aagcccggca
      961 gcgtaaacac caacatcacc attccacgaa tacccggcgc ttcaatgaac tgctcattac
     1021 agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc ttctggcagc
     1081 cgatagccat atacaattca gcagtggttt tacctggcag aatttcccgt agccactcga
     1141 ccagcgctgc gggcagcggt gcataaacca taaaataaga acgcgcaaag ccaaaaacaa
     1201 tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg tcgtccgtct
     1261 ggtggatcgg cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg cccaccagcc
     1321 aggcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt tccgcgaggt
     1381 tgtctgtccc cagcgtttcc gtcagatggc gaatgatgta atgaatgtca cggcttttat
     1441 tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg catcagtaga gattcccagc
     1501 cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag cggcgctctg
     1561 gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca aataaccgac
     1621 agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc ggcaacagcc
     1681 gcgtgtaatg ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg ccgttagtaa
     1741 tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg atacggtttt
     1801 tcatcgccag ctggacagca tgccagtcgg cctgttcgaa acgctgctgc gccccggaag
     1861 tcacttcgag gaatcgacca tactgagcgt cgaagccttg caaaatggtt tgagcaatca
     1921 gtaattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt gccggatgcg
     1981 gcgtaaacgc cttatccagc ctacgttcgg caacggttgt aggcatgata agacgcgcaa
     2041 gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtaaatgcct tatccggcct
     2101 acgttcggca acggctgtag gcatgataag acgcgcaagc gtcgcatcag gcatcgtgca
     2161 ccgattgccg gatgcggcgt gaacgcctta tccggcctac agtcagcaac ggttgttgtt
     2221 gcttagaact gcgattcttc agtggagccg gtcagcgcgg tgactgaaga cgtgccgccc
     2281 tgaataatag tcgtcacttt atcgaagtaa cctgtaccca cttcctgctg gtgagatacg
     2341 aaggtatagc catctttcgc ggcggcaaat tccggctgct gcactttctc aacgtagtgc
     2401 ttcataccct cgccctggtc ataggcgttt gccaggtcaa acatgttgaa ccacatgctg
     2461 tggatacctg ccagggtgat gaactggaac ttgtagccca tatccgacag ctgctgctgg
     2521 aagctggcaa tagttttgtc gtcgaggttt ttctgccagt tgaacgacgg cgagcagtta
     2581 taagccagca gtttgcccgg atatttcgcg tggatagctt gtgcaaagcg acgcgccagt
     2641 tccagatccg gcgtggaggt ttcacaccag accaggtcag catatggcgc atacgccagg
     2701 ccacggctga tcgcttgctc aatgcccgca tgagtacgga agaagccttc actggtacgc
     2761 tcgccggtaa taaattcgct gtcatacggg tcgcaatcgg aggtgatcag atccgcggca
     2821 tcggtacgag caaccagtag ggttggaacg cccgtcacgt cagctgccag acgcgccgcg
     2881 accagtttct gaatagcttc ttgagttggc actaaaactt tgccgcccat gtgaccgcat
     2941 ttcttcactg acgccagctg atcttcgaag tgaactgccg ctgcaccggc ttcaatcatc
     3001 gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg
     3061 atcggcagga agtaatcgac atagcgcgga tcgcccggct caatgcccgc ggaccattgg
     3121 atctgatccg cacggcggaa agtattgttg atccgctcca ccacagctgg caccgagttt
     3181 gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc
     3241 tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg
     3301 ccagtcagtg caccgaggct gttgatgtag cttttttcga ctcgccatgc agcagacgcc
     3361 acattctcgc cgcgcccagt tgcgccaggg tgcattcagg attgactgaa tcgcgtaatt
     3421 tcaccacatc ttccgcgctg tatgggcgag taatgccttc ccaacgcggt tgagtccact
     3481 ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc tccatagtta
     3541 tgtggtggtt tacgctaaca ggcagtagcc tggcagggtc aggaaatcaa ttaactcatc
     3601 ggaagtggtg atctgttcca tcaagcgtgc ggcatcgcca aaacgcccct gggagaaacg
     3661 ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct ggcggaacaa
     3721 ggctttagtt accggtttgc cattgctcaa cattttttga tgatggatcc actgccagat
     3781 tgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa tcggcacaca
     3841 gccgttgcca gagatccacg cttcgatgta ctgcacggct acgcgaatat ttgcgcgcat
     3901 accttcttcg gtgcgttcac cgtcacaagg tgccagtagc tgatcggcag taatcggcgc
     3961 gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt cgttgaatac
     4021 cgccattgcc gtatccgcaa ggcctgggtg agcgatccat gtgccatcgt gaccgttatt
     4081 ggcttccagc gatttatccg cttttacttt gttgagcacc tggttattgt gctcttcatc
     4141 tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt tatggcaggt
     4201 tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtca ctgcctgtct
     4261 gtctggcagg acgcgatcgg gatagttttt caacgttttg atatagctga agatgtaatc
     4321 ccaacgaccg cagttcagac caacaatatg gtcacgcagc gcgtgaagga tttcatccat
     4381 ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag
     4441 attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt cctgccagga
     4501 ctgggttttc ggcagataga aatagggacc actgcccttt gccaacagag cctgatagtt
     4561 gtggaagaaa tagagcgcaa aatcaaacag gctgccgggg attgcctcac cacgccaggt
     4621 gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg ctggattggg
     4681 cttgagctgg taaattttgc ctgcttcatt ggtatagctg atggtaccgt taactgcatc
     4741 acgcaggtta atttgcccgt cgatcacttt gttccagtcc ggggccagtg aatcttcgaa
     4801 atcggccata aagactttca cattggcgtt gagcgcgttg atcaccatct tgcgctctac
     4861 cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc gaattttcca
     4921 atcagcatcg caaatggaag ctgtttccga aataaaatca ggcaacgttc cgttatcaat
     4981 atcttgctgc tgctgaatgc gcgctgccag aagtttattt cgttgtggcg taaaatgcgt
     5041 caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct cctgctcgcc
     5101 atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca tcgtgcagct
     5161 cctcgtcatg gatccgaaaa cttccccact gtgaacgaag gatcgttgtg cgcttttcgt
     5221 tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc cattttaatt
     5281 gaaaatgctt gataacttat tgataacaat gtgaataaaa tttattcacc tcctgaggtg
     5341 cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga atcgcttaac
     5401 gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga tcgtatggcg
     5461 tgatctgata gacgtaatag ttgagccagt tggtaaacag caaattaccg tgactacgcc
     5521 agctcgctcg cggtgtattt tgcggatcat tgtgcgggaa atagttatac ggtacttccg
     5581 ggtctagtcc ggcttccaca tcgcggaaat attcctgcgc cagcgtttgc gcatcatatt
     5641 cgggatggcc cgtcacaaag gcaatgcgct tatctttgct ggcaaacaga tatgcatccc
     5701 cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac gctgccggaa
     5761 agtcagcata gcgcgaatgt ggtgccagga atgaatcatc aaagccacgc gtcagaagcg
     5821 catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg cgagtttgct
     5881 taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc ccagcagaca aacagcgtcg
     5941 aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag taagcgacat
     6001 cattaaactc caccaggccc agcggcgcac cggttacaat cagaccgtca aagttctgtt
     6061 cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc gtgttgcgcg
     6121 attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag tttgaaagca
     6181 ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg atcagaacct
     6241 taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag acgttttctt
     6301 cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aatcggcata acctgattac
     6361 ctcactacat acacttatac gtttagacat ccagatagct gaagataacc agaaaatttg
     6421 ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaagact acgttagaat
     6481 ataagaatga ctaaaaggag agaaaatggt tattagtatt cgccgctcac ggcatgagga
     6541 aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat gccacacacg attttttatc
     6601 agcagagtat cgggccgagc tggaggtgct agctagttcg ttccttcctg ccggaagcgc
     6661 cgttgtggat cgcggttaat gaacgggatc agccggttgg gtttatgttg ttaagtgggc
     6721 agcatatgga tgcgctgttt atcgatcctg atgtgtgcgg ctgcggcgtg ggtcggatgc
     6781 tggtggagca tgcgctctcg atggcaccgg aactgacaac caacgttaat gagcaaaatg
     6841 agcaggcggt tgggttctat aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg
     6901 acgatttggg gaaaccgtat ccgttgctga atctggcgta cgtgggggaa taagccgccc
     6961 ctgccgtgcc tgcccaggct ggagaagacg cgccagcgtc gcacccggca tttttt
//
LOCUS       DAJVOY010000192         6849 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVOY010000192 DAJVOY010000000
VERSION     DAJVOY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.192"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..167
                     /locus_tag="OQL90_003584"
     CDS             <1..167
                     /locus_tag="OQL90_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1370220.1"
                     /translation="VQSGIKVLGLAALDSTATPCYDRDTAQALVNVGAQIAAMTPGEL
                     ASWLAENLQS"
     gene            164..2164
                     /locus_tag="OQL90_003585"
     CDS             164..2164
                     /locus_tag="OQL90_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCS1370221.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAV
                     GFYRLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            2289..2750
                     /locus_tag="OQL90_003586"
     CDS             2289..2750
                     /locus_tag="OQL90_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS1370222.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2791..3261)
                     /locus_tag="OQL90_003587"
     CDS             complement(2791..3261)
                     /locus_tag="OQL90_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1370223.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3308..4027)
                     /gene="yehT"
                     /locus_tag="OQL90_003588"
     CDS             complement(3308..4027)
                     /gene="yehT"
                     /locus_tag="OQL90_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1370224.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(4024..5709)
                     /gene="btsS"
                     /locus_tag="OQL90_003589"
     CDS             complement(4024..5709)
                     /gene="btsS"
                     /locus_tag="OQL90_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1370225.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            5931..5996
                     /locus_tag="OQL90_003590"
                     /pseudo
     CDS             5931..5996
                     /locus_tag="OQL90_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            6224..6472
                     /locus_tag="OQL90_003591"
     CDS             6224..6472
                     /locus_tag="OQL90_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1370226.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(6588..>6849)
                     /locus_tag="OQL90_003592"
     CDS             complement(6588..>6849)
                     /locus_tag="OQL90_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1370227.1"
                     /translation="QHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQDAVD
                     LEIATEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
BASE COUNT         1736 a         1762 c         1674 g         1677 t
ORIGIN      
        1 gtgtccagag cggcatcaaa gtgctgggac tggcagcgct cgatagcaca gcaacacctt
       61 gctatgaccg cgatacggcc caggcgctgg ttaatgtcgg cgcacaaata gccgccatga
      121 caccgggcga gctggcatca tggcttgcgg agaatcttca gtcatgaatt cactacgtcc
      181 ggaattatta gaactgacac cgcaggccct gacggcgtta agcaatgccg gttttgttaa
      241 gcgcagtctt aaggaactgg aaaatggcaa cgtcccggag atcagccatg agaacggcgc
      301 tttaatcgcc accttcagtg acggtgtccg tacccagctg gcgaacggcc aggcactgaa
      361 agaggctcag tgcagctgcg gggccagcgg tatgtgccgt catcgcgtga tgctggtgtt
      421 aagttatcaa cgactttgtg ctaccgccca gcctacggga aaagaagagg aatgggatcc
      481 ggcaatctgg ctggaagaac tggctaccct tcccgatgcc acccgcaaac gcgcacaggc
      541 gctggtcgct aaaggcatca ccattgagtt gttctgtgcg ccgggtgaaa ttccctctgc
      601 ccgcttaccg atgagcgatg tgcgttttta ttcccgcagc agtattcgtt tcgcccgttg
      661 tgattgtatt gaaggcacac tttgcgaaca tgtcgtactg gcagtacagg cctttgtcca
      721 ggcaaaagcg cagcaagcgg aattgactca tttaatctgg cagatgcgca gcgaacacgt
      781 cacatcatct gacgatccgt ttgccagcga agaaggcaaa acgtgtcgtc aatatgttca
      841 acaactaagc caggcattat ggctgagcgg catcagccag ccgcttattc actacgaggc
      901 cgctttcagc cgcgcgcagc aagcagcgga acgctgcagc tggcgatggg tgagtgaatc
      961 gctacggcaa ctgcgcgcca gcgttgatgc cttccacgcc cgcgccagcc actatcatgc
     1021 cggagaatgc ttacgtcagc ttgcggcatt aaacagtcga ttaaactgcg cacaagagat
     1081 ggcccggcgc gacagtgttg gtgaagttcc tcctgtgccg tggcgcacgg tcgttggctc
     1141 tggcattgcc ggagaagcaa agcttgatca tctgcggctg gtgtctttag gtatgcgttg
     1201 ctggcaggat attgagcatt atggtttacg catctggttt accgatcccg acaccggcag
     1261 tattttgcat ctttcgcgta gttggccgcg aagtgaacag gaaaactcac cggcagctac
     1321 gcgtcggctg tttagttttc aggctggcgc actggcgggt gggcaaattg tttcacaagc
     1381 agcaaaacgc agtgccgatg gcgagctgct gttagctacc cgcaaccgct taagcagcgt
     1441 tgtgccgctg tcgccagacg cctggcaaat gttgagcgcg ccgttacgcc agccgggcat
     1501 tgtggctttg cgggaatatt tacgccagcg tccccccgcc tgcatacggc ctcttaatca
     1561 ggtggataac ttatttattc tgccggtcgc tgagtgtatt tcactcggtt gggacagcgg
     1621 ccgccagacg ctggatgcgc aggtaatcag cggcgaaggt gaagataatc tgctgacgtt
     1681 atcccttccg gcatctgcct gttctccttt tgccgttgaa cgcatggcgg cgcttttgca
     1741 acaaacagac gaccccgtga gtctggtttc tggctttgtc agctttgtag aggggcaatt
     1801 gacactggaa ccacgggtga tgatgacaaa aacccgtgcc tgggcgctgg acgcggaaac
     1861 tgcgcctgtg gcaccgctac cttctgccag cgttttgcct gtgccgtcta ccgctcatca
     1921 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
     1981 ggaacagagc gctattagtc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
     2041 ttatcggctg gcacatgtgt tggtacaatt tcgtaataca gaaagcgagg cacgggtaga
     2101 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
     2161 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
     2221 ttaggcatat cctatatagc ccctggtgag agtcaccagt ttcttgatta aataaaatgg
     2281 agttttacat gaaggctttc aataagctgt tttccctcgt tgttgcatct gttctggttt
     2341 tctctcttgc tggctgcggt gacaaagaag aatcgaagaa attcagcgcc aatctgaacg
     2401 gcactgaaat tgccattacc tatgtctaca aaggtgacaa ggtgcttaag caatcttctg
     2461 aaaccaaaat tcaatttgcc tccattagtg caaccaccaa agaagatgct gccaagacac
     2521 ttgagccgtt aagcgccaaa tacaaaaaca tcgcgggtgt tgaagaaaaa ttaacctata
     2581 ccgataccta cgcgcaggaa aacgtgacta tcgatatgga aaaagtggat tttaaagccc
     2641 tgcagggtat ttcaggaatc aacgtttctg ctgaagatgc caaaaaaggt atcactatgg
     2701 cgcaaatgga actggtgatg aaagccgctg gttttaaaga agtgaaataa tctgtcggcg
     2761 gccatgtttc gtatggccgc catacccgtc ttagcttttc ttcacatgct ggcgcgctgc
     2821 cagtccacgc agaaaataac gtaaaaattg atcgccgcat tcgcggaagt ttttatgatc
     2881 cggtgcgcgc atcatcgctg taatttccgg catcgaaacg cggaactgct gttcggtgag
     2941 gatagccaga atgtcatcgg ttttcagcga aaacgcgatg cgtaattttt tcagcacgat
     3001 gttgttatta atgcgacgtt ccggctccag tgccggagca gactcatcct tgccgcgttt
     3061 ttcataaatc aggccattga ggaatgacga caaaacaatg tccggacaac gctgaaaacc
     3121 ctcttcgtct tctttacgta gccagacggc gatctgttcc gcggtggctt cgacattacc
     3181 cagcgccaga atacgcacca ggtcattatt attggctttc aaaatgtagc gcacgctgcg
     3241 cagaatatcg ttacttagca tgaggccttc aggtgttgat gaggcaaaaa gccattttag
     3301 cagtctttta caggccaatc gcctctttta agcttttcag ataacggcgg ctgaccggca
     3361 cggttaagcc attacgcaaa atcaactcgg cctggccgtt atcttccaga cgaatctcct
     3421 gtaaatgcgc gaggttaacc agatactgac gatggcagcg cagtagtggt gtacgacttt
     3481 ccagggtacg taatgtcaat tcggtaaagc cctctttccc ttcgtggctg gtaacgtaga
     3541 caccgctcat ccgactgctg acaaatgcca catctttcat ttgcagcaaa taaatccgac
     3601 tatgccccgt acaagggata aatttcagcg cctgttgatt ttccggtaac agcgaaacat
     3661 cctgcttgct gcgctcctga cgcaatcgcg ccagcgtttt ctccagtcgc gcttcatcaa
     3721 ttggcttcag cagataatca aaggcatgtt cttcaaaggc tttaatagcg tattcgtcaa
     3781 acgcagtgag aaaaacaata tacgggcgat gttccgggtc aagcatcccc accatttcca
     3841 gaccactgat gcgcggcatc tggatatcga gaaacagcac atccgggcgc agtttatgca
     3901 ccgcgccgat cccttccacg gcgtttgaac actctccaac gatttcaata tcgctctgct
     3961 cctgcaaaaa tacacgcagg ttctcccgtg ctaacggttc atcatcgaca attaagactt
     4021 taatcatgcc tcgtccctcc atggtagttg taacgttatt cgggtgtaac tatcaggctc
     4081 acaggcgacg cttattccat agtcatcgcc aaaccgttca cgtaaacgct tatccaccag
     4141 attcatcccc agcccactgg cattggttac cggttgatac aaaccggcat tgtcttcgat
     4201 ctccagcatc aaatgttgcc cctcacgtcg ggcgctgatt gccactcgcc ctgtatccag
     4261 cagttgtgat gtcccatgtt taatggcgtt ttccactatc ggttgcaggg taaacgcggg
     4321 caattgctgc tgggataatt cttgcggaat agcaatgttg acctgcaacc gcgactggaa
     4381 gcgcgccttt tcaatttgca gataagcgtt cacatgttca atttcgtcgg cgagagtaac
     4441 aaactccgaa ggccgcttta agtttttgcg gaaaaaagtg gaaagatact gcaccagctg
     4501 gctggcctgt tcgctgtcgc ggcggatcac cgctttaatg gtgttaagcg cattaaacaa
     4561 aaaatgagga ttcacctggg cgtgaagcag tttgatttct gactgggtga gcatcgcttt
     4621 ttgccgctca tattgcccgg caagaatctg cgccgaaagc agttgcgcaa tcccctcgcc
     4681 cagcgtgcgg tttattgaac tgaataaacg gtttttggct tcatacaatt tgatggtgcc
     4741 catcacccgc tgattttcac cacgcaacgg aattaccagc gtcgacccca gtttgcattg
     4801 cggatgcaaa gagcaacgat aaggtacttc gttgccatca gcgtagacca cttcaccggt
     4861 ttcaatcgct tttaaggtgt aagtcgaaga aatcggtttg ccgggtaaat ggtggtcgtc
     4921 accaattcca gtaaaggcca gcaatttctc tcgatcggta atcgcgactg caccaatatc
     4981 cagctcctga tacagcacct gtgccacttt catgctgttc acttcgttaa acccctggcg
     5041 caaaatgcct tccgtcgagg cagccacttt cagcgcagtg gcagaaaaag ccgaagtgta
     5101 tttttcaaac atcgcgcgtt tatcgagcaa tatacgcata aacagcgccg cgccgacggt
     5161 attggtgacc atcattggtg cagcaatatt actcaccaga cgcaccgcat cttcataagg
     5221 tcgggcgatc gccaggatga tcaacatttg caccatttca gcgacgaacg tgacggcacc
     5281 ggcggtaatg gggttaaaga ctttatcagt acgcccgcga cgaatcagga tgctgtgtac
     5341 caggccgccg agcaatcctt caacgatggt cgagatcatg caacttagcg cggtcatgcc
     5401 ccccatcgaa tatcgatgta acccgccggt caggccaacc agcccaccga cgaccggacc
     5461 gccgagtaag ccgcccatta ccgcgcctat cgcacgggta ttggcaatag aatcgtcaat
     5521 gtgcaaccca aaccaggtgc ccatgatgca gaagatggaa aagacgatgt agcagagaaa
     5581 tttatgcggc agacgaaccg tgacctgcat taacggtatg aataatggcg ttttactcat
     5641 taaccacgca atgactaaaa aaacgcacat ctgctgaagc agcagcaaca ccagattaaa
     5701 atcgtacata cccgcaaacc acacttccct ttaaaacgcg taacatacat tgcctgcgtt
     5761 taactttctt tgaactcttg cagaaaaatg agaattcgtg agtacgatca ctcaaaatcg
     5821 cctggcaaaa ataaaatcac cctatagatg cacaaaaaac gggcaaaact acctggttcg
     5881 caaaactgcg tctaaagtta aaccgggacc tcgcgagcaa gggtgagacg atggcgcttt
     5941 acacaattgg tgaagtggcg ttgctttgtg atattaaccc tgtcacgtta cgcgcgtgac
     6001 gcaacatttt aaaaaatcga cgaagatcgt ccaggagcgc cgtttttcaa gggttggata
     6061 gatgattcgt ttaccatgtc gacgaacttc gacgaatttc gccgataaaa agtgtcccat
     6121 acatgcccca ttgatgcacc ataaacaata tcagaaattg atactggtta tccatacaga
     6181 taaaaataac acctgtacat aactacagtt acaggagggt gttatgcgtg ttgagatctg
     6241 tatcgctaaa gaaaaaatca ctaaaatgcc aaacggtgct gtggatgcgt taaaggaaga
     6301 attaacccga cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag
     6361 caacgatggc ctttctgtta cacgcaccgc agataaggat tctgcaaaaa catttgttca
     6421 ggagactctg aaagatacct gggagtctgc tgacgagtgg tttgttcact aataaacacg
     6481 taaaatcggt aacggctgga aatcattcaa tactcgcact atcgaaagtt taccagccaa
     6541 ccgcagcacg tcctgcatac gtcgtgtcta cggtttttct ttttcgctta cattgtgtct
     6601 ggttcttccg gccactcaat atcaggtgca gttgatgtat caacacagtt cagcaacacc
     6661 cgatacttct tccaggcttc cagcaacgag ttttcttcct ccgttgcgat ctccagatct
     6721 acagcatcct gaagtggcgc aatatgctca ctgaattcct ggatgtagaa ctgtgtggtg
     6781 acggtcttcc agccattcgg ctcctgctgt atcgaagcat accaggctat ttcaatatcg
     6841 ctatgctgc
//
LOCUS       DAJVOY010000193         6802 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVOY010000193 DAJVOY010000000
VERSION     DAJVOY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.193"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            187..2103
                     /gene="dnaK"
                     /locus_tag="OQL90_003593"
     CDS             187..2103
                     /gene="dnaK"
                     /locus_tag="OQL90_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1370228.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2192..3322
                     /gene="dnaJ"
                     /locus_tag="OQL90_003594"
     CDS             2192..3322
                     /gene="dnaJ"
                     /locus_tag="OQL90_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1370229.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3426..3578)
                     /gene="mokC"
                     /locus_tag="OQL90_003595"
     CDS             complement(3426..3578)
                     /gene="mokC"
                     /locus_tag="OQL90_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1370230.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4164..5330
                     /gene="nhaA"
                     /locus_tag="OQL90_003596"
     CDS             4164..5330
                     /gene="nhaA"
                     /locus_tag="OQL90_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1370231.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLCPSV"
     gene            5396..6295
                     /gene="nhaR"
                     /locus_tag="OQL90_003597"
     CDS             5396..6295
                     /gene="nhaR"
                     /locus_tag="OQL90_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1370232.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6333..>6802)
                     /locus_tag="OQL90_003598"
     CDS             complement(6333..>6802)
                     /locus_tag="OQL90_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1370233.1"
                     /translation="RFNYNLNTILLVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHI
                     RFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDG
                     TNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1739 a         1600 c         1757 g         1706 t
ORIGIN      
        1 tctgcgcaaa agcacaaaaa atttttgcat ctcccccttg atgacgtggg ttacgacccc
       61 atttagtagt caaccgcagt gagtgagtct gcaaaaaaat gaaattgggc agttgaaacc
      121 agacatttcg cccctattac agactcacaa ccacatgatg accgaatata tagtggagac
      181 gtttagatgg gtaaaataat tggtatcgac ctgggtacta ccaactcttg tgtagcgatt
      241 atggatggca ccactcctcg cgtgctggag aacgccgaag gcgatcgcac cacgccttct
      301 attattgcct atacccagga tggtgaaact ctggttggtc agccggctaa acgtcaggca
      361 gtgacgaacc cgcaaaacac cctgtttgcg attaaacgcc tgattggccg ccgcttccag
      421 gacgaagaag tacagcgtga tgtttccatc atgccgttca aaattattgc tgctgataac
      481 ggcgacgcat gggtcgaagt taaaggccag aaaatggcac cgccgcagat ttctgctgaa
      541 gtgctgaaaa aaatgaagaa aaccgctgaa gattacttgg gtgaaccggt aactgaagct
      601 gttatcaccg taccggcata ctttaacgat gctcagcgtc aggcaaccaa agacgcaggc
      661 cgtatcgctg gtctggaagt aaaacgtatc atcaacgaac cgaccgcagc tgcgctggct
      721 tacggtctgg acaaaggtac tggcaaccgt actatcgcgg tttatgacct ggggggtggt
      781 actttcgata tttctattat cgaaatcgac gaagttgatg gcgaaaaaac cttcgaagtt
      841 ctggctacca acggtgatac ccacctgggt ggtgaagact tcgacagccg tctgatcaac
      901 tacctggttg aagaattcaa gaaagatcag ggcattgacc tgcgcaacga tccgctggca
      961 atgcagcgcc tgaaagaagc ggcagaaaaa gcgaaaatcg aactgtcttc cgctcagcag
     1021 accgacgtta acctgccgta catcactgca gatgcgaccg gtccgaaaca catgaacatc
     1081 aaagtgactc gtgcgaaact ggaaagcttg gttgaagatc tggtaaaccg ttccattgag
     1141 ccgctgaaag ttgcactgca ggacgctggc ctgtccgtat ctgatatcga cgacgttatc
     1201 ctcgttggtg gtcagactcg tatgccaatg gttcagaaga aagttgctga gttctttggt
     1261 aaagagccgc gtaaagacgt taacccggac gaagctgtag caatcggtgc tgctgttcag
     1321 ggtggtgttc tgactggtga cgtgaaagac gtactgctgc tggacgttac cccgctgtct
     1381 ctgggtatcg aaaccatggg cggtgtgatg acgacgctga tcgcgaaaaa caccactatc
     1441 ccgaccaagc acagccaggt gttctctacc gctgaagaca accagtctgc ggtaaccatc
     1501 catgtgctgc agggtgaacg taaacgtgcg gctgataaca aatctctggg tcagttcaac
     1561 ctggatggta tcaacccggc accgcgcggc atgccgcaga tcgaagttac cttcgatatc
     1621 gatgctgacg gtatcctgca cgtttccgcg aaagataaaa acagcggtaa agagcagaag
     1681 atcaccatca aggcttcttc tggtctgaac gaagatgaaa tccagaaaat ggtacgcgac
     1741 gcagaagcta acgccgaagc tgaccgtaag tttgaagagc tggtacagac tcgcaaccag
     1801 ggcgaccatc tgctgcacag cacccgtaag caggttgaag aagcaggcga caaactgccg
     1861 gctgacgaca aaactgctat cgagtctgca ctgactgcac tggaaactgc tctgaaaggt
     1921 gaagacaaag ccgctatcga agcgaaaatg caggagctgg cacaggtttc ccagaaactg
     1981 atggaaatcg cccagcagca acatgcccag cagcagactg ccggtgctga tgcttctgca
     2041 aacaacgcga aagatgacga tgttgtcgac gctgaatttg aagaagtcaa agacaaaaaa
     2101 taatcgccct ataaacgggt aattatactg acacgggcga aggggaattt cctctccgcc
     2161 cgtgcattca tctaggggca atttaaaaaa gatggctaag caagattatt acgagatttt
     2221 aggcgtttcc aaaacagcgg aagagcgtga aatcaaaaag gcctacaaac gcctggccat
     2281 gaaataccac ccggaccgta accagggtga caaagaggcc gaggcgaaat ttaaagagat
     2341 caaggaagct tatgaagttc tgaccgactc gcaaaaacgt gcggcatacg atcagtatgg
     2401 tcatgctgcg tttgagcaag gcggcatggg cggcggcggt tttggcggcg gcgcagactt
     2461 cagcgatatt tttggtgacg ttttcggcga tatttttggc ggcggacgtg gtcgtcaacg
     2521 tgcggcgcgc ggtgctgatt tacgctataa catggagctc accctcgaag aagctgtacg
     2581 tggcgtgacc aaagagatcc gcattccgac tctggaagag tgtgacgttt gccacggtag
     2641 cggtgcaaaa ccaggtacac agccgcagac ctgtccgacc tgtcatggtt ctggtcaggt
     2701 gcagatgcgt cagggtttct ttgccgtgca gcagacctgt ccacactgtc agggccgcgg
     2761 tacgctgatc aaagatccgt gcaacaaatg tcatggtcat ggtcgtgttg agcgcagcaa
     2821 aacgctgtcc gttaaaatcc cggcaggggt ggacactgga gaccgcatcc gtcttgcggg
     2881 cgaaggtgaa gcgggccagc atggcgcacc ggcaggcgat ctgtacgttc aggttcaggt
     2941 taaacagcac ccgattttcg agcgtgaagg caacaacctg tattgcgaag tcccgatcaa
     3001 cttcgctatg gcggcgctgg gtggcgaaat cgaagtaccg acccttgatg gtcgcgtcaa
     3061 actgaaagtg cctggcgaaa cccagaccgg taagcttttc cgtatgcgcg gtaaaggcgt
     3121 caagtctgtc cgcggtggcg cacagggtga tttgctgtgc cgcgttgtcg tcgaaacacc
     3181 ggtaggcctg aacgagaagc agaaacagct gctgcaagag ctgcaagaaa gcttcggtgg
     3241 cccaaccggc gagcacaaca gcccgcgctc aaagagcttc tttgatggtg tgaagaagtt
     3301 ttttgacgac ctgacccgct aacctcccca aaagcctgcc cgtgggcagg cctgggtaaa
     3361 aatagggtgc gttgaagata tgcgagcacc tgtaaagtgg cggggatcac tccccgccgt
     3421 tgctcttact cggattcgta agccgtgaaa acagcaacct ccgtctggcc agttcggatg
     3481 tgaacctcac agaggtcttt tctcgttacc agcgccgcca ctacggcggt gatacagatg
     3541 acgatcaggg cgacaatcat cgccttatgc tgcttcattg ctctcttctc cttgacctta
     3601 cggtcagtaa gaggcactct acatgtgttc agcatatagg gggcctcggg ttgatggtaa
     3661 aatatcactc ggggcttttc tctatctgcc gttcagctaa tgcctgagac agacagcctc
     3721 aagcacccgc cgctattata tcgctctctt taacccattt tgttttatcg attctaatcc
     3781 tgaagacgcc tcgcattttt gtggcgtaat tttttaatga tttaattatt taactttaat
     3841 ttatctcttt atcgcaatta ttgacgacaa gctggattat ttttgaaata ttgtcctaac
     3901 aaataacgcc gactgacaag aaattaatta ttacttttcc taattaatcc ctcagaaatc
     3961 ctcaccttaa gctatgatta tctgagctta gggtcactcg tcagcgctta cagccgtcaa
     4021 aaactcatct caccgctgat ggcgaaaatt ctacaaaggc tcgtaaaaaa cgaattattc
     4081 ctacactata atctgatttt aacgatgatt cgtgcggggt aaaatagtaa aaacgattta
     4141 ttcacctgaa agagaaataa aaagtgaaac atctgcatcg attctttagc agtgatgcct
     4201 cgggaggcat tattcttatc attgccgcta tcctggcgat gatgatggcc aacagcggcg
     4261 caaccagtgg atggtatcac gactttctgg agacgccggt tcagctccgg gttggttcac
     4321 tcgaaatcaa caaaaacatg ctgttatgga taaatgacgc gctgatggcg gtatttttcc
     4381 tgttagtcgg tctggaagtt aaacgtgaac tgatgcaagg atcgctagcc agcttacgcc
     4441 aggccgcatt tccagttatc gccgctattg gtgggatgat tgtgccggca ttactctatc
     4501 tggcctttaa ctatgccgat ccgattaccc gcgaagggtg ggcgatcccg gcggctactg
     4561 acattgcctt tgcacttgga gtgctggcgc tgttgggaag tcgtgttccg ttagcactga
     4621 agatcttttt gatggctctg gctattatcg acgatcttgg ggccatcatt atcatcgcat
     4681 tgttctacac taatgactta tcgatggcct ctcttggcgt cgcggctgta gcaattgcgg
     4741 tactcgcggt attgaatctg tgtggtgtac gccgcacggg cgtctatatt ctggttggcg
     4801 tagtgttgtg gactgcggtg ttgaaatcgg gggttcacgc aaccttggcg ggggtaattg
     4861 tcggcttctt tattcctttg aaagagaagc atgggcgttc tccagcgaag cgactggagc
     4921 atgtgttaca cccgtgggtg gcgtatctga ttttgccgct gtttgcattt gctaatgctg
     4981 gcgtttcact gcaaggcgtc acgctggatg gcttgacctc cattctgcca ttggggatca
     5041 tcgctggctt gctgattggc aaaccgctgg ggattagtct gttctgctgg ttggcgctgc
     5101 gtttgaaact ggcgcatctg cctgagggaa cgacttatca gcaaattatg gcggtgggga
     5161 tcctgtgcgg tatcggtttt actatgtcta tctttattgc cagcctggcc tttggtagcg
     5221 tagatccaga actgattaac tgggcgaaac tcggtatcct ggtcggttct atctcttcgg
     5281 cggtaattgg atacagctgg ttacgcgttc gtttgtgtcc atcagtgtga caggacggtt
     5341 taccggggag ccataaacgg ctcccttttc attgttatca gggagagaaa tgagcatgtc
     5401 tcatatcaat tacaaccact tgtattactt ctggcatgtc tataaagaag gttccgtggt
     5461 tggcgcagcg gaggcgcttt atttaactcc acaaaccatt accggacaga tccgggctct
     5521 ggaagagcgc ctgcaaggca aattatttaa gcgcaaggga cgtggtctcg aacccagcga
     5581 gctgggagaa ctggtctatc gctatgccga taaaatgttc accttaagcc aggaaatgct
     5641 ggatattgtg aactatcgca aagaatccaa tttattgttt gacgttggcg tggctgatgc
     5701 actttccaaa cgcctggtca gtagcgtgct gaatgctgca gtggtagaag gcgagccgat
     5761 tcatcttcgc tgcttcgaat ccacccacga aatgctactg gagcaattaa gccagcataa
     5821 actggatatg atcatttccg actgtccgat agactctacg cagcaggaag gcctgttctc
     5881 cgtgagaatt ggcgaatgtg gcgtgagttt ctggtgtaca aatccaccac cagaaaaacc
     5941 gttcccggct tgtctggaag aacggcgact tttgattcct gggcgacgtt caatgttagg
     6001 gcgcaaattg cttaactggt ttaactccca gggattaaac gtagaaatcc tcggcgagtt
     6061 tgatgatgcc gcgttgatga aagcttttgg tgcaatgcac aatgcaatct tcgttgcccc
     6121 aacgctttat gcatatgact tttatgccga taaaactgtc gtagaaattg gtcgcgtcga
     6181 gaatgtgatg gaagagtacc atgctatttt tgctgagcgg atgattcagc acccggcggt
     6241 acagcgaatc tgcaatacgg attattctgc gctttttagt ccagcggcgc gttaatcggc
     6301 agctccccca aagttaaagt ggaggagata gattagttgt acataaccac gattttgact
     6361 cggctcatta tttgcccgct tgagacatta tttccatatg tacgcgggcg aataaataga
     6421 ggaatctgat tacttccttc atggggatgc tgaaaagagt agtaattgct tttaccatac
     6481 tggatttcat tcgtaccatc attggagagt tgaaagccta cttgacccgc accgtcctca
     6541 ctgttttcta actcattttt taatatgctg gttcctgact cctgtatcgt cactggttca
     6601 aagtaatatt caacatcact gggttcaaaa actaattcac cttgctcgcc ctggatgcag
     6661 tcaaatctta tatgatgaaa ctgataattt tcattattga tacgagacag tacaaatcgt
     6721 ccagtgtcga tggattgatc ttcaaaacca caggtcatta aatttaccag taaaatagta
     6781 tttaaattat aattaaagcg ga
//
LOCUS       DAJVOY010000194         6779 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVOY010000194 DAJVOY010000000
VERSION     DAJVOY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.194"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OQL90_003599"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OQL90_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1370234.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OQL90_003600"
     CDS             2089..2238
                     /locus_tag="OQL90_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370235.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2994..3434)
                     /locus_tag="OQL90_003601"
     CDS             complement(2994..3434)
                     /locus_tag="OQL90_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1370236.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3652..4041
                     /locus_tag="OQL90_003602"
     CDS             3652..4041
                     /locus_tag="OQL90_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370237.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4085..4489
                     /locus_tag="OQL90_003603"
                     /pseudo
     CDS             4085..4489
                     /locus_tag="OQL90_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4596..5432)
                     /locus_tag="OQL90_003604"
     CDS             complement(4596..5432)
                     /locus_tag="OQL90_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1370238.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5432..5707)
                     /locus_tag="OQL90_003605"
                     /pseudo
     CDS             complement(5432..5707)
                     /locus_tag="OQL90_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5782..6255)
                     /locus_tag="OQL90_003606"
     CDS             complement(5782..6255)
                     /locus_tag="OQL90_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCS1370239.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6276..6779)
                     /locus_tag="OQL90_003607"
                     /pseudo
     CDS             complement(6276..6779)
                     /locus_tag="OQL90_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1582 a         1755 c         1771 g         1671 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttttacatca ttccttcagc tcatacgtca
     2641 ctttcatcct ctgtgccatt tgtacatcac ccccttcctg aacgtgctga cagcgggttt
     2701 tccggctgcg ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt
     2761 tcggatggga agaacgccgg aaacaggact taccccggct gaatggtgtg aatttccgcg
     2821 ctatgcactt gtgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg
     2881 ggttttcggg tggtttgttg cctgttttac cggtttcccg tcagaaacgc cctgagggcc
     2941 tctcaggcgg tgctgataac agatgttatg gtaaatcaga gtcacctgca ctgttactct
     3001 gaaccggagc gaaacagccg cgaccagaat ccccgctttt tttcctgtgc cagcgcgtca
     3061 gccagacggc taatttcggc ctgtagttgt tccctctcct catttcgcag gcggttttct
     3121 gcttcccgcg ccatcttgtc ttcaagcatt agggtaacag tctgctgcag agcgtccagt
     3181 ttctgctgaa tgacgtctag ctgagcattt tccgcctggc taacgacaga tttggagtgt
     3241 ccctccttgt gcctttttgg tgtctcagaa gtgcgcagct cgccataggc ccgaatcagt
     3301 tcactggtct cgaactcccg gcgatcattt tttcctgtgc gatagctgac aaggccttta
     3361 gccatgtcac gatataattg cgagcgcgac tttcctgtta atttcagtgt ctcttgaact
     3421 gtgtgccatg ccataaaagt gacagtgtct cctgtgtgta ccgtattgta acagcagaac
     3481 atattactgt tttaacacaa aaggtctgag gctgcccgtg aggcgtgaaa aatacacaaa
     3541 aaacctgttg acggtgtggt tttgacgggc gtagtatttc gaatactgta tataaacaca
     3601 ttgttataaa tacagttttt gctgttttta aaagtcaaag aggataattt tatgagtaca
     3661 gagttaaata gcggtactgt cggtggtttt ggtcacatgg gaccaaatga cccctacaat
     3721 gatggtcata gcggctctgg aagttcggga ggtagtttta acggtggtgg taatggtggc
     3781 catgataata gtagtggttc atcttatact gcatcctttg gtctcatcac tggccgacac
     3841 ccgataactg tcccaggtac gaagaatatt aattctgtcg atctttctgc tgtcattaat
     3901 aacattatac atcatgaagg tgcacgcaat aacatgtacc tagatactgc gaacaatgta
     3961 agagtgggag tgacgggcac tggctggcaa tgtctagcaa cggcaggcat ttcggctgag
     4021 ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg
     4081 gaacatgcct catgtggcgg ccaggacggc cagccgggat cgggatactg gtcgttacca
     4141 gagccaccga cccgagcaaa cccttctcta tcagatcgtt gacgagtatt acccggcatt
     4201 cgctgcgctt atggcagagc agggaaagga attgccgggc tatgtgcaac gggaatttga
     4261 agaatttctc caatgcgggc ggctggagca tggctttcta cgggttcgct gcgagtcttg
     4321 ccacgccgag cacctggtcg ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg
     4381 ggcgcggcgg atggccgaaa gtgccgcctt gctggttgat gaagtactgc ctgaacaacc
     4441 catgcgtcag tgggtgttga gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg
     4501 cgggaagggg cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga
     4561 tgtgttccca ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc
     4621 ttgatcgcgg ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct
     4681 gcggaaaggc acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc
     4741 gcacgagaga atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct
     4801 ctgtcagccg gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg
     4861 acgggatcta tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg
     4921 ccatgtagcc cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg
     4981 tggacgtagt cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct
     5041 gcaaagcgat cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac
     5101 agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg
     5161 atgtgagaga gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg
     5221 agcaaccgga ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca
     5281 tcagcaatgt cttctatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat
     5341 gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc
     5401 caatgagcag gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca
     5461 gatagaaggc aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag
     5521 cgaaggcgcg ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga
     5581 gtgccaaatc ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc
     5641 attgaagagt tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa
     5701 ccatatcgtc gctatcaatc gcggaaaaag gcgtagtgcg cgaagcgaac gaagcttttt
     5761 gccgtcgatt gcatagcttt gttaaccctt tttccaaatt tgatagcaat agttaatgtt
     5821 tgaagtaaag tgttgctcaa aaacaacttc gaaggtattt ggaatactcg ggaagaaaac
     5881 atccccctct ggctcgatgt cgatcgtcga taagtggagc gtagaggcca tgggtaatgt
     5941 ttctcggtaa atttctccgc caccagacac tataacgtga ccggtgaatt cagctagcct
     6001 gtccatggcc tcttcgattg actgaaatac aactacattg tcatcatttg atgtccaacc
     6061 tgagcgggta acgaccgcgt atttcctatt ggggagtgcg cccatagatt caaacgtctt
     6121 gcgacccacc agaagccact gattgtaggt caatgcttta aaaagtagct gctccccttt
     6181 cgcggaccag ggtatgtctg gaccgcaacc aatcacgccg tttttcgctt tcgcagctat
     6241 caatgatact ttcaaggttc tcatcctggg ttaacggtgc gctcttggtc gagccgcacc
     6301 ggtagctcta gttcgacacg agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc
     6361 ggtaagaagt cgggattgac ggcattgcgg gacaccacat caacggcgcg tccgaacatt
     6421 cgcgacagcc tgcgctcaaa cggacattga tcaaccgata ggctgtgaac agcgccaagt
     6481 tgctgcccca ttgacggcca cgctttgagc aaatccgctc cagacagatc agccgccggt
     6541 actcccggaa ttgccgttat caccaagcat gcaccctcct gttcctcctg ccagttgatg
     6601 acctcggggc aagccacacc tcgacctttg agccaaatga ggcggtcacg ctctccagcg
     6661 agctcaccgc ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga
     6721 aaaacaaaat caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacc
//
LOCUS       DAJVOY010000195         6759 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVOY010000195 DAJVOY010000000
VERSION     DAJVOY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.195"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..297
                     /locus_tag="OQL90_003608"
     CDS             <1..297
                     /locus_tag="OQL90_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1370240.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(448..3771)
                     /gene="mscM"
                     /locus_tag="OQL90_003609"
     CDS             complement(448..3771)
                     /gene="mscM"
                     /locus_tag="OQL90_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1370241.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3793..4761)
                     /gene="psd"
                     /locus_tag="OQL90_003610"
     CDS             complement(3793..4761)
                     /gene="psd"
                     /locus_tag="OQL90_003610"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1370242.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(4858..5910)
                     /gene="rsgA"
                     /locus_tag="OQL90_003611"
     CDS             complement(4858..5910)
                     /gene="rsgA"
                     /locus_tag="OQL90_003611"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1370243.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6005..6550
                     /gene="orn"
                     /locus_tag="OQL90_003612"
     CDS             6005..6550
                     /gene="orn"
                     /locus_tag="OQL90_003612"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1370244.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1563 a         1817 c         1813 g         1566 t
ORIGIN      
        1 cttccttacc tgttcctcgc gctggctttc ccattcttta aagcacgtca ggatctcgac
       61 agaccatttg tgattttcaa aacgcgtatg tcggcaatga ttgcgacggt ggttgtcgta
      121 ctggtggtga catttgcgaa cgtcttcacc attattccgc ctgtggttga agccggagac
      181 tgggacagca cattgtggat gattggcggc cctgtcttct tctcgctgtt agcgatggcg
      241 atttaccaga actattgcag tcgcatggcg aataaacctg agttagctct cgactgatca
      301 tatgaaatcc tgcaactcat cgtctctccg gcgatgagtt gtcaggaagt aatgcgtatg
      361 gatgatactc tccagggccg gtgataactg aagatgatgt taagctgcta aatgagccac
      421 gttgacatca tttggcagct tcatgcatta caaacttccc gcctgacgac ctttgcccgc
      481 agacgtcagc gttctccccg tttgtttacc gttgaggctt tccagacgca tctggaaggg
      541 cgggaatggc atatcgatac cgtgggcatg gaagccagcc agaatcagct ggtggatctc
      601 atggcgtagc ggcatacggt gacccatctc agcggcataa atacgcagct cgaaaatctg
      661 aatcccttgt tgcagatcca ccaggaagac ttccggtgcc gggttgtcga tcaccagcga
      721 gcagcgacgc gctgcggtga gcaggatttc cgtcacttct tcactgttgg catcagcagg
      781 ggccggaatc gtcaacacta cgcgcgtaac cgagtcagag agcgaccagt tgataaactg
      841 ctcggtaata aacgccttgt tcggcacgat tatctcttta cggtcccagt cgctgatggt
      901 ggtggcgcgg gtgttaattt tcgtcacgct accggtgaga tcgcgaattg tcaccgtatc
      961 gccaatgcga atcggttttt cgaacaggat aatcagacca gagataaagt tggcgaaaat
     1021 ttcctgcaaa ccaaaaccga gaccaacacc gagcgcggcg accagccact gcaatttcga
     1081 ccattcaata ccaatcatcg agaagccgac cagcccgcca atcagcatca gcagatattt
     1141 ggtgatggtg gtgatggcgt agcctgtacc cggcgttaaa tccaggtgct gcaaaatcgc
     1201 cagttccagc agcgcgggca ggttgcgcac cagctgcgtg gtgatgataa acaccagaat
     1261 ggcaatcagc accgcaccga gggtaattgg ctccagactt tctacgcctt gtaccgtgga
     1321 ggtgacatcc cacagcgaaa tattttcgag gaagccgaaa gcggaatgga tttctgacca
     1381 cagcacaatg acagaaagca gggcgatcaa catcaaaatt gagcgcacca gccgcaagga
     1441 ttgcgcactg atggcatcga gatcgacttc gctttcatcg acttcaattg ctccttccgg
     1501 gctactgtga tgatgcgctt cctcttcgcc acgcgcacgt tgcgctaaca tctctgcccg
     1561 gcgatgcttc gcccggtcaa acgccagcct gcggcgctgg atcagcatcc agcggcggat
     1621 aacgtgataa accaccagta gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa
     1681 cagcgcctgt gccgttgcca gataacccac agccgacgcc agaatggcaa ccaacggcgc
     1741 gccaatcatc atgttccaca gcatatggtt ggtaatgttg tcgccgctgc cctctttgtt
     1801 gagatacagt gggatcccgg cctttttcag gctgagggtg accaccgcca gcgcaccgca
     1861 aatgaggata aagcaaagcc gtcccagcga accggagaat tcacggtcgt cgaggttatc
     1921 gaacatcatc agcgccataa tcagcggcac aataagcccg atgctcatca ggtagtagcg
     1981 catcccacgg gaaacacgtt cgcgcggcca gccaaaatga gcgataaaca agccgttcgg
     2041 gcgggcaaag gtggcgcaaa tcatcactac ccacagcagc ggcacggtag ccgttacacc
     2101 atcgccaatc gcgaccgcca gcggataagg ccacgcctcg cgcaagccgt aacccagcgt
     2161 catccacagc accggtaacg gtgacgcgac gagaatcgac cagaaaagag tgcgcaacgt
     2221 cagccagaag tgatcctgag tcactttgcc aactttcgcc gccgaacgtt caagaaaacg
     2281 ggtgaaatag cggcgcgagt aaatactgca accgaccaga atcaacgcgc caaacagcgg
     2341 caaaattgtc tctttgctgg tcagcatcat cacactggct ttgcccaact gactgaaggt
     2401 gtccagcgaa atgagacgac gcagatcctg ggcgatttcc agcggccagg cgatggtcat
     2461 cgggcgcacg tcagaggtcc agaacagata gcggtgcgtt gcttcgttca cctctttcag
     2521 cgcatcctcc agttgcccgt tggagacttt cagcttggtc agttccaata gtagcgtgtc
     2581 gccaccctgc aacaatgagt tcagcaactc acgctgagtg cgcagttgtg cttccagaat
     2641 acggttttgc tcggcggtca gcggctgacc gtcggcctga tggatttgcc gtagcagcgg
     2701 ctgtttatta agcagatcct cataacgtaa ccgttgcaca cgcaactgcg ccatttcggt
     2761 atcaagctgt tgtggttttg gcatttctgg cagccgtgcc acctgcgccc gcagcgcttc
     2821 gccgagcaga ttggacgatc ccagccattg cgactgttca cgcagcgtat tcaacgcctg
     2881 ccggacctgt aacgtctggc tggcagcctg acgctgttgc gaggcaacga gatccatccg
     2941 ctgcgcctgt tgattcaaag ccgccgatag ttcgcggtta attttgaatt gcgcgacgat
     3001 atctttcggc aaatcggcgc tgttttctgc cagcaattcg gtactttcca gcgcccgctc
     3061 cgcctcaagc tgacgttggc tgtttaattg attacgcaag gcctgcaaat acgcatccag
     3121 ttgctggctc tctttttccg ccagctctga gcgtaagcgc gctaattcct ggcggttatt
     3181 ggcagacagc tgcgccagct ccagttcatc aacgagcgcc ttaagacgtg cagagtcaga
     3241 ctgcaacgcg aaattttgtg cctgattgag cggagtattg ccggtaagcg ttcccaggcg
     3301 gcgctcgatc tcatttaact gacggcgggc gtcggtttgc tgttgcggca gttgattcag
     3361 cgaatcggca atctcgcggg tgcgctcctg ctcttgctgg gcctgacggc ttttatccag
     3421 caactggctg ctgacctgga gaatttcctg attcagcgcg tcggtagaca ttcccggcga
     3481 cacgctgcgc ggctcgtcac gcatgttgtt taattgtgcg cgcagagtag cggagagttt
     3541 cggataatta tcgataactt gctgatattg tttgatgcgc tcaagggaac cttttcgttc
     3601 ctcaagcgca tttaaggcag actggagcgc ctctacgact tccggctgtg cgggtttcgc
     3661 cgcttttgcc tgctccagtt cctgagtgat ttgtttgcta tcgggggccg tcgcggcgta
     3721 cgccccccaa ctgaggcacc aggccatcag aaaagtgata atcaggcgca cgtcagcgtt
     3781 tcctttgatg gattagacct ggtctttttt atcgtcaacc aatgggctgg cgtcgtgttc
     3841 tgcttcgatc tcttcagcag gaagtggggc gggttcagcg tctggcgtaa caaaggtttc
     3901 ggtagatact gccagcggct ggccaatttt cgtgacagac aggctttcca gttgctcaac
     3961 cagattcact ttacccggtg caaacaggtt gataacggtg gaaccgagtt taaagcgacc
     4021 catttcctgg cctttcagca gtgccacaga accgtcgttt tccccggcag gccaggtcca
     4081 gcgcttgatg ataccttcgc gcggcggcgt aatggtgccc gcccagaccg tctcaatgct
     4141 gccaacaatc gtcgctccga ccagaatctg cgccattggg ccaaattcgg tatcgaaaag
     4201 gcaaatcacg cgttcgttac gggcaaacag attcggcacg ttctgagcgg tgagatggtt
     4261 aacggagaag agatcgcccg gcacgtagat catctcacgc agaataccgt tgcacggcat
     4321 gtgtacgcga tggtagtcac gcggggagag gtaagtggtc acaaacgtac cgttgcggaa
     4381 caggtccgcc atcagatagt tgcctgccag cagggcttcg aggctgtagt tgtggccttt
     4441 ggcttgcagg attttatctt cttcgatttt acccaactgg ctgataacgc catcggcagg
     4501 catgaccagt acattcggat cggtatcgat tgggcgtact tcgtcacgca gcggacggac
     4561 aaagaattcg ttaaaggtgc ggtagctggc ggtgtccggc ttttgcgcct ctttcatgtc
     4621 gaccttgtag tatttaacga acagatcgat aaccagtttt gtcagccatc ctgcccgctt
     4681 gcttgcgccc caacccgcca ggcgagtaag ccatagtttc ggcagaatgt actgtagcga
     4741 aagtttaaat gaatttaaca aggtagcctc caggccattg ttttgtcgtt cctgatccgg
     4801 ccaacatgcc ggatcctgaa aaaaagggga cgattctaac gacggttagc ttaattgtca
     4861 gtcatccgta tcagaaaagt ttttacgcgt ttttacctgc gccatgcttt ccagaatacg
     4921 gtgatagttt tcgaaacggg tttccgcgat tttcccttcc tcaaccgctt cccggatagc
     4981 gcagcccgga tcggtatcgt gtttgcaatc gcgatattta cacagaccta aatagtcatg
     5041 gaattcgaca aagccctgag tgatttgttc cggctccagg tgccagaggc cgaactcacg
     5101 cacaccaggg gaatcaatca catcaccgcc gtgcgggaag tgatacagcc gagcggcggt
     5161 cgtggtgtgc tgaccgagcc ccgagttgtc agagacatcg ttggtcagga tctctttttg
     5221 cagccccagc aacgcattca gcaggctgga tttaccgacg ccagactgcc cggcaaaaat
     5281 gctgatgcgc ccggtcaacg cctcttccag cggttttagc ccatcctgag tatggctgga
     5341 aaccatcaat acgcgataac cgatattgcg gtagatatcc atctgctcgt tgacgaacgc
     5401 catgccttcg tcgtccagca ggtctatctt gttgagcaca ataatcggct caatctgcaa
     5461 ggtttcgcag gccaccaggt aacggtcgat aatattgagt gacagctccg gcaaaatggc
     5521 ggagacaatg acaatctggt cgatgttggc ggcaataggt tttacgccgt cgtagaaatc
     5581 cggacgcgtc aacaccgagg tacgctcatg caccgcttcc acgatccctt tgacatttac
     5641 gccttccgcc gccggtttac ccggacgcca gactacacgg tcgccggtca ccagcgaacg
     5701 gatggtacgg cgaatattgc agcggtgaac gtcgccatcg gcggattcca catcagcgtg
     5761 cataccaaag cggctgatga cgataccttc atcaggctcg ccaaacagat tgtcgtcgta
     5821 gtcgggcttc tccttagacg ttttaagacg acgctggtga ttggcgttca cgcggcgctg
     5881 ctggcctttg gagagtttat ttttactcaa tcgtacagac tcctggtcgc ccctgatggg
     5941 caaaacatct atgatacacg caattgtgga tcaatatagt cactgtgaat gggtggaaaa
     6001 tagcatgagt gccaatgaaa acaacctgat ttggatcgat cttgagatga ctggtctgga
     6061 tcccgagcgc gatcgcatta ttgagattgc cacgctggtg accgatgcca acctgaatat
     6121 tctggcagaa gggccgacca ttgcagtaca tcagtccgat gaacagctgg cgctgatgga
     6181 tgactggaac gtgcgcaccc ataccgccag cgggctggta gagcgcgtga aagcgagcac
     6241 gatgggcgat cgggaagctg aactggcaac gctcgaattc ttaaaacagt gggtgcctgc
     6301 gggaaaatcg ccgatttgcg gtaacagcat cggacaggac cgtcgtttcc tgtttaaata
     6361 catgccggag ctggaagcct acttccacta ccgttatctc gatgtcagca ccctgaaaga
     6421 gctggcgcgc cgctggaagc cggaaattct ggatagtttt accaagcagg ggacgcatca
     6481 ggcgatggat gatatccgtg aatcggtggc ggagctggct tactaccgcg agcattttat
     6541 caagctgtaa aatttttcct ggtcacgtaa gcgccggaat ccacttgcgg cggcgtgatt
     6601 ttgacgctaa attgaacgtc ttgctgataa tttcagcact tgagataaaa acgcaaaaaa
     6661 aacttttttg gggggttgca gagggaaaga tttctcgtat aatgcgcctc ccgtaacgac
     6721 gcagaaatgc gaaaattacg aaagcaaaat taagtagta
//
LOCUS       DAJVOY010000196         6722 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVOY010000196 DAJVOY010000000
VERSION     DAJVOY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.196"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            164..892
                     /gene="mpaA"
                     /locus_tag="OQL90_003613"
     CDS             164..892
                     /gene="mpaA"
                     /locus_tag="OQL90_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1370245.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPSSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(867..1832)
                     /gene="ycjG"
                     /locus_tag="OQL90_003614"
                     /pseudo
     CDS             complement(867..1832)
                     /gene="ycjG"
                     /locus_tag="OQL90_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            1951..2457
                     /gene="tpx"
                     /locus_tag="OQL90_003615"
     CDS             1951..2457
                     /gene="tpx"
                     /locus_tag="OQL90_003615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1370246.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2501..4042)
                     /gene="tyrR"
                     /locus_tag="OQL90_003616"
     CDS             complement(2501..4042)
                     /gene="tyrR"
                     /locus_tag="OQL90_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1370247.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4190..5251)
                     /locus_tag="OQL90_003617"
     CDS             complement(4190..5251)
                     /locus_tag="OQL90_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1370248.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5248..6645)
                     /locus_tag="OQL90_003618"
     CDS             complement(5248..6645)
                     /locus_tag="OQL90_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1370249.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1606 a         1915 c         1642 g         1559 t
ORIGIN      
        1 ctaagtcagc ataattctaa tatctgcaca tcaataaaaa tgcaaacaac ttccggcaaa
       61 aaaagtgata caagatcgat gacatagcgt tgaagaaaag gatttgataa tccgcaaaat
      121 ttgcacaaca ctgcctgtaa tacttcctga accagagacg atcatgaccg taacccgccc
      181 acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat tacggacgtt cattattggg
      241 cgcgccgtta atctggtttc ctgcccctgc agccagccgc gaaagtggtt tgattctggc
      301 tggcactcac ggtgatgaaa actcttcggt cgtcacgctc tcctgtgctc tgcggacgtt
      361 gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg gctgccagct
      421 ggggttacgg gccaatgcta atggtgtgga tttaaacaga aactttccgg cggcgaactg
      481 gaaggaaggt gaaacggttt atcgctggaa cagcgccgct gaagaacgtg atgtcgtatt
      541 actgacaggt gacaaacctg gctcagaacc tgaaacccag gcgctgtgcc agcttataca
      601 ccgcattcaa cctgcctggg tggtctcatt ccacgatcca ctggcctgta ttgaagatcc
      661 cagacacagc gaattaggcg aatggctggc ccaggcgttt gaattgcctc tggtaaccag
      721 cgtcggttat gaaacgccta gctctttcgg cagttggtgt gcagatctca acttgcattg
      781 catcaccgca gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat atctctttgc
      841 gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt cgcccgtcgt gaactgaagc
      901 gccggttcca catctaccgc cagccaggtc ggtccgtcaa gatcggcgaa actgacctgc
      961 ggcaccagcg gtaaagcggc gctaatggca cgagaggtac acaacatgca gcccagcatc
     1021 agactgaaac cttgtgcacg cgcttcagtc gccagcgcca gcgcttccgt cagaccaccg
     1081 gttttatcga gcttaatgtt aaccatctca tagcgccctt tcagcgcctt cagattgcta
     1141 cgtgtatgac aactctcatc agcacaaatc ggcaacggat gaataaaatt ctccagcgcc
     1201 gcatcgtcct gcgcaggaag gggttgttca agcatcgcaa cgcctaaatc cgccaatagc
     1261 tggcaacgcg ccgccaaccc ttatgcacgc caggattcat ttgcatcaac gatcagcgtc
     1321 gcatcgggca cagctgtgcg aattgccacc atccgctcac taataagatg gttatccagc
     1381 ttcactttca gtaatttcgc gcctgcctgc cagagtgttg atgcactatt ggccatctga
     1441 tcaggcgtac cgatgactac cgtctgtgca gtgataactg tctcgggaag cgttatgccg
     1501 atcaaatcag ccagcgattg ctgctgtttt cgcgcggcca gatcccacaa tgcacaatcc
     1561 agcgcattac gtgctgcgcc ggcagggaga attttttgca actcctcccg tgtcagccct
     1621 ttctctaatt gcgacacgac gctcataatt tgcgccatta ccgaggcatc actttctcca
     1681 taacgcggat acggcgtgca ttcgccggtg cctttaatac cctcttcttc cagttcaacc
     1741 accaccacgc gcgcttcact gcgacttccc cgggcaatca caaacggggt atgtaagggc
     1801 taggcttcct caaatacctt aacggttctc atctttactc cttgttgtcg cggtggtctg
     1861 cggattaagc gcaaatagcg tttgctgtgt tattgacagt tagcataaac taggtgtgac
     1921 gttaactata tgtaaacagg aaagataatc atgtcacaaa ccgttcattt ccagggcaac
     1981 ccggttacag tcgccaattc catcccgcag gcgggtagca aagcgcagac ttttactctc
     2041 gtggcaaaag atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg caaagtgctg
     2101 aacattttcc cgagtattga taccggtgtt tgcgccgcat cagtacgtaa gtttaaccaa
     2161 ctggcaaccg agatcgacaa caccgttgtg ctgtgtattt ctgccgatct gccgttcgcc
     2221 cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta tcaccctctc cactttccgt
     2281 aacgctgaat ttctgcaagc cttcggtgtg gcaattgctg atggcccact gaaaggtctg
     2341 gcagcgcgtg ccgttgtggt tattgacgaa aatgacaatg tgattttcag ccagctggtg
     2401 gatgaaatca ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa agcataagtt
     2461 aatatgcctg atggtgttgc accatcaggc atattcgcgc ttactcttcg ttcttcttct
     2521 ggcttaagcc atattcccgc aacttattgg caatcgcggt atgtgaaacg ccgagacgtt
     2581 ttgccagttt gcgcgtgctg ggataattgc gataaagctg ggttaatacc gagcgttcaa
     2641 aacggctggt gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg gctaccgttg
     2701 cggcgtcata atccggcaac aaaatatcct gtggacgcag ctcataaccg tccagttgtg
     2761 tcagtgcgcg atatatagcg ttctttaact gccgcacatt tcccggccac gcataacgcg
     2821 taagtacagt attcaggtca gcggccagtt tcggacgcgg cacgccctgc tcgtcggcaa
     2881 agcgggcgac gaacagctca gttaacggca tgatgtcctg cggacagtca cgtagcggcg
     2941 gcagattgag cgtcaacacg ttcagacgat aatagagatc ttcacggaac atgccttttt
     3001 gcaccagttc gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg acatgcacct
     3061 catggtcttc gccaacccga cggaaagtgc catcattaag aaaacgcagt aatttcgcct
     3121 gcatccgtgg tgacatttcc cctatttcat ccaacagcac cgaaccaccg ttcgcctgct
     3181 caaagaatcc tttcttccct tccggagcat gaccaaacag ttcactctcg accgcatctt
     3241 ccggtataga cgcacagttc agcgccaggt aaggtttgcc cgctctgggg cttgcctgat
     3301 ggcaggcgta ggcaaagaga tctttacctg tacctgtgtc acccgtaatc agcagcggcg
     3361 cgcttagcat cgccagtttc tgcgcctgtt cgacaacatg cttcattttc gggctgacgg
     3421 cgacaatttg actgaaggcg ctgacgtcct gggcggcgac attttgcaac tggcggccca
     3481 tacgaatcgt tgatcgcaac atcaccaccg caccggtcag gacgtgttga tcattttcat
     3541 cctgaagata aacaggcgta atctccatca ggaaattctg cccattaata acgacatgct
     3601 cgttatgcga atcttgcggt tcgctttcca gccaacgtaa aaaattaaag ccgttaatca
     3661 attgtgcggc ggtatggttg cgcaggcgat ccaatttttg cccaaaaagc tgacagctcg
     3721 ccgggttcgc catatccact ttgcttttca tatcgacaga gagcacaggt tcgggcaacg
     3781 cctccagtaa cgcgctcaac gccagatgct cacgttcgga aggcatccac gggacagtac
     3841 gcacatcggt aacacctgca atacggcgta tttcggccat cagactgctg aaactctcaa
     3901 actccagttc agcaaaattg aggtagattc gcccaatggg atcaatctca ataccgcgta
     3961 aatcaatgcc tcttagcacg agtagatcga gtaattcgcg ggtcagaccg agtcggtctt
     4021 cacaaaagac ttccagacgc atgggaacct tcacctgaaa aaagaacaat taatatgatg
     4081 atatgacact atttggatag caggaaggtt tctgtcaaca atcattgaca caaagacgga
     4141 aaaacgggca cttcggcgta aagatatccg gtcagcaccc gatattgcat tatttttcgc
     4201 tgggcgtttt gcctttttgc agcgtttctt tcacctgacc gataagctga cgacggaaat
     4261 ccccgaggcg aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc tccatagctt
     4321 taatcccgag tcgtgccgtc agaagtccag caccaattcc ctgagctgcg cgggtagaca
     4381 aacgagcagc gagatcttgc gacatccagt ccatccccac ttcgcgcacc agttcgctgg
     4441 ctccggcaaa agcgatattc agcaatacca gcttaaacag acgcaaacgg ctgtaatacc
     4501 ccagttcaat gccatacagc gtggcgatgc gattaattaa acgcagattg cgccaggcga
     4561 taaacgccat atcgaccaac gccagcgggc tgaccgcaat catcaacgtt gattccgccg
     4621 ccgaacggca gatttcgcgc cgcgcctggg catctaaaac tggctggacc aaatgcgcat
     4681 acaaactgac cacttcacgg tcgttttgcg tttcatggat tgaggcatac cagcgttgca
     4741 gcgctggatg agactgatca atacccgcct gctgcgccag tttttcgcaa aatgcgcggc
     4801 ctttgcccgt gccgtggctg tgcaacaaat cgcgcgcttc gtcgcgttca tgggcgcgct
     4861 gtcgcaagcg ccataagcgc cgccactctg ttaccacaga acctacgcca gcgccgataa
     4921 tcaatgcccc agcggcacat ccacccagcg ccacccagtc ctgagtttgc caggcattca
     4981 ttgtccactg tacaccctgc ccgacaacgc ttgcgccaaa cagagccagc ccgcccatca
     5041 ccattttgcg ccacaggctg cgtttcggac gtaacgctgc atccattacc gcttcaactt
     5101 gcccctcttc ttcaggcgct tcatcgagcg tggccggggc aaaattttgc gcctgatttt
     5161 cgtcaaaggt ctgctgcgcc ctgaattttg gattctgatc gacctccagc ggaccgtcga
     5221 aatcaatacg tggttttaac ggttcggtca tcgcaattta tctcctatta aaaattccag
     5281 cgcggcatca agacgaatat gcggcagcgg tttgtcgaca tccatcacct gcgggcgaaa
     5341 cgcttcaaac tggaaccctt gtttgtccca gaacgcctga ccgggcaaac gcgcaggaac
     5401 ttcaccggga taaacggtga gtggcgcacc atcgctgagc cggttaccgc gcaatgccgg
     5461 aattttctcg ccgttgacgt caataatgcc gctggtggtc gcctgcaccg aggccagccc
     5521 caggcaatcc atactaatcc cttcaaacgc cgcattttgc caggcatcct gaatcagttg
     5581 ttgcagtaat gaaaccatat tggcgtgctg atcgatggtc acatggtccg ctttagtggc
     5641 agcaaacaat agcttatcga taaccggcga aaacaaacgc cggaacaagg tacgctgccc
     5701 gtagtggaaa ctttgcatca gctgcgtcag tgccagacgc atatcattaa atgcctgtgg
     5761 cccactgttg agaggttgca ggcaatcaac cagcacaatc tggcggtcaa agcgcagaaa
     5821 atgattctta tagaacccct tcaccacctt ctcgcagtaa taattaaacc gctcgcgcag
     5881 cattccggca ttggtgtgct tatcggcctg cgccagtttg gactcgcccc aggtatcgac
     5941 atccggccac gggaagaatt gcagcgcggg cgcacctgcc atatctcctg gcaagacaaa
     6001 gcgccctggc tgaataaagt gcagcccctg ctgtttacag tggtggagat aatcggtcca
     6061 cgcggcggca atgtctgcca gccggttttc gtcggcaggt gctagcgggt ccagcccttc
     6121 gcacatcatt cgccatttga ccgaccattc gccgcgctga ccattgagta agcccgtcat
     6181 ctggcgtgac cagcttaaat agtcctgcgc cagcatcggc aggtcgagca accattcgcc
     6241 gggataatcc acaatctcca gatacagcgt ggaggtgtcc ttgaagtggc gtagcagcga
     6301 atcgttcgat ttaaaacgta gcgccaggcg gatttcactg acgccgcgcg ttggcgtcgg
     6361 ccaggcggga ggatcgccat ataactgcgc cagtccttca tcatatgtga agcgcggaat
     6421 gccaaagtca cgctgaggaa tgcgttttac gcccagcagg cgctcttcac gcaccgcgct
     6481 taacagcggc aagcgtgctc cggcgtgaat attgagcaac tgattgacca tagcagtgat
     6541 aaacgctgtt ttgccgctgc ggcttagtcc ggttacggcg aggcgcagat gtctgtcgac
     6601 accccgattc accagcgcat taagttcatt tttaagtcgc ttcattgctg tcctgtgctg
     6661 ctctgaagac cagaaaaaaa tcattcgtaa ggatatgaat gtgggggcca ggccattgat
     6721 ta
//
LOCUS       DAJVOY010000197         6700 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVOY010000197 DAJVOY010000000
VERSION     DAJVOY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.197"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            185..1555
                     /locus_tag="OQL90_003619"
                     /pseudo
     CDS             185..1555
                     /locus_tag="OQL90_003619"
                     /inference="COORDINATES: protein motif:HMM:NF015188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1715..2548
                     /locus_tag="OQL90_003620"
                     /pseudo
     CDS             1715..2548
                     /locus_tag="OQL90_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2676..3398)
                     /locus_tag="OQL90_003621"
     CDS             complement(2676..3398)
                     /locus_tag="OQL90_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1370250.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3545..6172
                     /gene="gyrA"
                     /locus_tag="OQL90_003622"
     CDS             3545..6172
                     /gene="gyrA"
                     /locus_tag="OQL90_003622"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1370251.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDAAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6321..>6700
                     /locus_tag="OQL90_003623"
     CDS             6321..>6700
                     /locus_tag="OQL90_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HCS1370252.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVMHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLD"
BASE COUNT         1600 a         1666 c         1913 g         1521 t
ORIGIN      
        1 aaacgttggc tccacccaac aaacctttgt gcactcattg acgggctttc agaatggcac
       61 tttaaatatc gatgctggtg gcaatgttac tgttaatcaa ggtagttttg ctggcaccat
      121 cgaaggtgct ggtcagctca ccattgcgca aaacggcagc tacgtgctgt cgggggcgca
      181 gtcgatggcg ctaaccggcg atatagtcgt tgatgatggt gcggtgtttt cgctggaagg
      241 cgacgcggca gatctcgccg ctctccagga cgatccgcag tcgatcgtgt taaacggcgg
      301 tgtgctcgat ctctctgatt tctccacctg gcagagcggc acatcataca atgatggcct
      361 tgaagtcagt ggcagcagcg gaacggttat cggcagtcag gatgtggtag atcttgcagg
      421 tggcgacaat ttgcatatcc gcggcgacgg gaaagatggc gtctacgtgg tggtcgatgc
      481 gagcgacggg caggtaagtc tggcaaacaa taatagttat ttgggcacaa cacaaatcgc
      541 ctccggtacg ctgatggtga gcgacaactc gcagcttgga gatacccact ataaccgcca
      601 ggttatcttt accgataagc aacaagaaag cgtgatggag attactgcca atgtcgatac
      661 tcgctctacg acgactgagc atgggcgtga tattgaaatg cgcgccgacg gtgaagtggc
      721 agttgatgcg ggggtagaca cgcagtgggg cgcactgatg gctgacagca gcgggcagca
      781 tcaggatgag ggtagcacat ttactaaaac gggggcgggt acgctggagc tgaccgccag
      841 cggtacaacg cagtcggcag tacgtgtcga agaaggcacc ctgaaaggcg atgttgcgga
      901 tatctttcct tatgcttcgt cactgtgggt cggtgatggg gcgacgttcg ttactggcgc
      961 ggatcaggat attcagtcta tcgatgctac ttccagcggc actatcgata tcagcgatgg
     1021 tacggttttg cgtctgaccg ggcaggatac ttccgtcgcc cttaatgcct cattgtttaa
     1081 cggcgatggg acgctggtga atgctaccga tggcgtgacg ttgacaggtg agcttaatac
     1141 caaccttgaa actgacagcc tgacctatct tgccgacgtg acggttaatg gtgatctgac
     1201 caatacgtcc ggtgcggtta gcctgcaaaa tggcgtcgct ggcgatacgc taacggtaaa
     1261 cggtgattat actggcggcg gtacgctact gttcgacagc gaattaaacg gcgatgactc
     1321 ggtaagcgat caattggtga tgaacggtaa tactgctggc aacacaactg tggtggttaa
     1381 ctccattaca gggattggtg agccgacatc gacaggcatt aaagtggttg atttcgcagc
     1441 tgatcccacg cagtttcaaa acaatgcaca gttcagtctg gctggcagtg gctacgtcaa
     1501 catgggagcg tatgactaca cgctagtgga agataacaac gactggtatc tgcgatcgca
     1561 agaagtaacg ccgccatcgc cacctgatcc agacccggac ccaacacccg atcctgaccc
     1621 aacacccgat ccaatccccg cttaccagcc ggtgctgaat gccaaagttg gcggttatct
     1681 caataacctg cgggcggcaa atcaggcgtt tatgatggag cgacgcgatc acgctggtgg
     1741 cgatggtcag acgctgaatt tacgtgttat cggcggacgt tatcattaca cggcggcagg
     1801 gcaactggct cagcaggaag acacttctac ggtgcagctt agcggcggct tgtttagcgg
     1861 gcgctggggc acggatggcg agtggatgct tggggcggtt ggtggctaca gtgataatca
     1921 gggcgacagc cgctcgaata tgaccggaac tcgcgccgat aaccagaacc acggttatgc
     1981 cgttgggctg acctcaagct ggtatcagca cggtaattag aagcaggggg cctggctgga
     2041 tagctggctg caatacgcgt ggtttaacaa tgatgtttcc gaacatgaag atggcgtgga
     2101 tcgttaccat tcgtcgggga ttatcgcctc gctggaagcc gggtatcagt ggttaccggg
     2161 gcgtggtgtg gtgattgaac cgcaggcgca ggtgatttat caggacgtgc agcaggatga
     2221 ttttaccgcc gctaaccatg cgcgcgtgtc acaatcgcag ggggatgata ttcagacgcg
     2281 gctgggttta cacagcgaat ggcgtaccgc ggttggtgtc acaccaacat tagatctgaa
     2341 ttattatcac gatccccatg cgacggaaat tgaagaagat ggcagcacta tcagtgacga
     2401 tgcggtgaag cgacggggtg aaataaaagt gggagtcacg ggcaatatca gtcagcgagt
     2461 gtccctgcgc ggcagcgtgg cgtggcagaa agggagtgat gattttgccc agacggcagg
     2521 gtttttgtcg atgacggtga aatggtaatc actgtctagt cggcacggtt gccggatgcg
     2581 gcgtaaacgc cttatccggc ctacggggag catttgtagg cctgataaga cgcggcaagc
     2641 gtcgcatcag gcattggctc ggccaaacaa caacctcact tattctgcgt gtgcagcata
     2701 tagttcacat ccacgccggg gccgagttta aaagtattag tgagcgggtt gtaatgtagc
     2761 ccagttatat gtcgctcttt aaggcttgtc tgatctaccc agccaagcag ttctgccggt
     2821 ttaataaact tcttcacatc atgcgtgcct ttgggcacca tgcgcaaaat atattccgcg
     2881 ccaaccaccg ccatcagcca tgacttgccg ttgcggttaa gcgtcgagaa aaagacatcg
     2941 ccgcctggtt tcaccagttg cgcacaggct ctgaccactg actgcggatc ggggacgtgc
     3001 tccagcatct ccatgcaggt caccacatca tactgcccgg catgttttgc cgcgtgctct
     3061 tccacggttt cctgcacata atccacctga atgccgcttt ccagcgcgtg cagttttgcc
     3121 acctgcaatg gctcaaagcc catatccaga ccggtcaccg tcgcgccttc gcgcgccata
     3181 ctctcggcca gaatgccgcc gccacaaccg acatcgagca cctttttgcc aaataaaccg
     3241 ccagcacgct cggcaatata gcccagacgc agcgggttaa tgcggtgcaa cggtttgaac
     3301 tcaccttcaa gatcccacca gcgggaggcg acggcttcaa atttagcgat ctcttcgtgg
     3361 tctacgttat ggttttccgg cgatttttcg gcattcattg gcacttctac tccgtaattg
     3421 gcaagacaaa cgagtatatc aggcattgga tgtgaataaa gcgtataggt ttacctcaaa
     3481 ctgcgcggct gtgttataat ttgcgacctt tgaatccggg atacagtaga gggatagcgg
     3541 ttagatgagc gaccttgcga gagaaattac accggtcaac attgaggaag agctgaagag
     3601 ctcctatctg gattatgcga tgtcggtcat tgttggccgt gcgctgccag atgtccgaga
     3661 tggcctgaag ccggtacacc gtcgcgtact ttacgccatg aacgtactag gcaatgactg
     3721 gaacaaagcc tataaaaaat ctgcccgtgt cgttggtgac gtaatcggta aataccatcc
     3781 ccatggtgac gcggcggttt atgacacgat cgtccgtatg gcgcagccat tctcgctgcg
     3841 ttacatgctg gtagacggtc agggtaactt cggttccatc gacggcgact ctgcggcggc
     3901 aatgcgttat acggaaatcc gtctggcgaa aattgcccat gaactgatgg ccgatctcga
     3961 aaaagagacg gtcgatttcg ttgataacta tgacggcacg gaaaaaattc ccgacgtcat
     4021 gccaaccaaa attcctaacc tgctggtgaa cggttcttcc ggtatcgccg taggtatggc
     4081 aaccaacatc ccgccgcaca acctgacgga agtcatcaac ggttgtctgg cgtatatcga
     4141 tgatgaagac atcagcattg aagggctgat ggaacacatc ccggggccgg acttcccgac
     4201 ggcggcaatc attaacggtc gtcgcggtat tgaagaagct taccgtaccg gtcgcggcaa
     4261 ggtgtatatc cgcgctcgcg cagaagtgga agttgacgcc aaaaccggtc gtgaaaccat
     4321 tatcgtccac gaaattccgt atcaggtaaa caaagcgcgc ctgatcgaga agattgcgga
     4381 actggtaaaa gaaaaacgcg tggaaggcat cagcgcgctg cgtgacgagt ctgacaaaga
     4441 cggtatgcgc atcgtgattg aagtgaaacg cgatgcggtc ggtgaagttg tgctcaacaa
     4501 cctctactcc cagacccagt tgcaggtttc tttcggtatc aacatggtgg cattgcacca
     4561 tggtcagccg aagatcatga acctgaaaga catcatcgcg gcgtttgttc gtcaccgccg
     4621 tgaagtggtg acccgtcgta ctattttcga actgcgtaaa gctcgcgatc gtgctcatat
     4681 ccttgaagca ttagccgtgg cgctggcgaa catcgacccg atcatcgaac tgatccgtca
     4741 tgcgccgacg cctgcagaag cgaaaactgc gctggttgct aatccgtggc agctgggcaa
     4801 cgttgccgcg atgctcgaac gtgctggcga cgatgctgcg cgtccggaat ggctggagcc
     4861 agagttcggc gtgcgtgatg gtctgtacta cctgaccgaa cagcaagctc aggcgattct
     4921 ggatctgcgt ttgcagaaac tgaccggcct tgagcacgaa aaactgctcg acgaatacaa
     4981 agagctgctg gatcagatcg cggaactgtt gcgtattctt ggtagcgccg atcgtttgat
     5041 ggaagtgatc cgtgaagagc tggagctggt tcgtgaacag ttcggtgaca aacgtcgtac
     5101 tgaaatcacc gccaacagcg cagacatcaa cctggaagat ctgatcactc aggaagatgt
     5161 ggtagtgacg ctctctcacc agggctacgt taagtatcag ccgctttctg aatacgaagc
     5221 gcagcgtcgt ggcgggaaag gtaaatctgc cgcacgtatt aaagaagaag actttatcga
     5281 ccgactgctg gtggcgaaca ctcacgacca tattctgtgc ttctccagcc gtggtcgcgt
     5341 ctattcgatg aaagtttatc agttgccgga agccactcgt ggcgcgcgcg gtcgtccgat
     5401 cgtcaacctg ctgccgctgg agcaggacga acgtatcact gcgatcctgc cagtgaccga
     5461 gtttgaagaa ggcgtgaaag tcttcatggc gaccgctaac ggtaccgtga agaaaactgt
     5521 cctcaccgag ttcaaccgtc tgcgtaccgc cggtaaagtg gcgatcaaac tggttgacgg
     5581 cgatgagctg atcggcgttg acctgaccag cggcgaagac gaagtaatgc tgttctccgc
     5641 tgaaggtaaa gtggtgcgct ttaaagagtc ttctgtccgt gcgatgggct gcaacaccac
     5701 cggtgttcgc ggtattcgct taggtgaagg cgataaagtc gtctctctga tcgtgcctcg
     5761 tggcgatggc gcaatcctca ccgcaacaca aaacggttac ggtaaacgta ccgcagtggc
     5821 ggaataccca accaagtcgc gtgcgacgaa aggggttatc tccatcaagg ttaccgaacg
     5881 taacggttta gttgttggcg cggtacaggt agatgactgc gaccagatca tgatgatcac
     5941 cgatgccggt acgctggtac gtactcgcgt ttcagaaatc agcatcgtgg gccgtaacac
     6001 ccagggcgtg atcctcatcc gtactgcgga agatgaaaac gtagtgggtc tgcaacgtgt
     6061 tgctgaaccg gttgacgagg aagatctcga taccatcgac ggcagtgccg cggaagggga
     6121 cgatgaaatc gctccggaag tggacgttga cgacgagcca gaagaagaat aattttactt
     6181 cttcatgcca aaagggagct atctcccttg tttgaattga aaagtccagg ctgcaaagtc
     6241 tgggcttttg tcgtattagg gcacggtaaa gtttggctgt gcccgtaaaa aatggctggc
     6301 tatacacaag gaatgtggca atgagtggtg aaaaaaaggc gaaaggctgg cggttctatg
     6361 gtcttgtagg ttttggcgca atagtactgc tttccgctgg cgtctgggcg ttgcaatatg
     6421 ctggcagtgg gccagaaaaa acgttgtcgc cgctggtgat gcacaacaat ctgcaaatcg
     6481 atctcaatga gccggacctc tttctcgaca gcgactctct gagccagctt cccaaagatc
     6541 tcctcaccat tccgtttctc cacgatgttc tgagtgaaga tttcgttttc tattatcaga
     6601 atcatgccga tcgtctgggc attgaaggca gcattcgtcg cattgtctat gaacacgatc
     6661 tcacgctgaa agataaactc ttttcgtcac tcttagatca
//
LOCUS       DAJVOY010000198         6686 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVOY010000198 DAJVOY010000000
VERSION     DAJVOY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.198"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            157..465
                     /locus_tag="OQL90_003624"
     CDS             157..465
                     /locus_tag="OQL90_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1370253.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(524..1618)
                     /gene="yaiW"
                     /locus_tag="OQL90_003625"
     CDS             complement(524..1618)
                     /gene="yaiW"
                     /locus_tag="OQL90_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1370254.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(1631..2851)
                     /gene="sbmA"
                     /locus_tag="OQL90_003626"
     CDS             complement(1631..2851)
                     /gene="sbmA"
                     /locus_tag="OQL90_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1370255.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            3203..4360
                     /gene="ampH"
                     /locus_tag="OQL90_003627"
     CDS             3203..4360
                     /gene="ampH"
                     /locus_tag="OQL90_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1370256.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(4361..4984)
                     /gene="iprA"
                     /locus_tag="OQL90_003628"
     CDS             complement(4361..4984)
                     /gene="iprA"
                     /locus_tag="OQL90_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCS1370257.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(5072..>6686)
                     /locus_tag="OQL90_003629"
     CDS             complement(5072..>6686)
                     /locus_tag="OQL90_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1370258.1"
                     /translation="SQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSYSTV
                     NLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNV
                     ISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTEL
                     DNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKADLGA
                     YTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADN
                     GGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMND
                     RSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAW
                     GFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDA
                     GYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKN
                     FSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         1688 a         1716 c         1602 g         1680 t
ORIGIN      
        1 ccattttgtg actcacaacg aagattcacg cttcgggaat attcccaaca tcaatttacc
       61 atgattctga tattgacaat ctaaatgacc tgtcagactt gccataagcc gctggtatca
      121 caccagaaga gaagcagatg acttccggag gtttttatgg ctgatttcac cctgtcaaaa
      181 tcgctgttta gcggaaaata tcgcaatgcc tcttcaacgc ctggcaacat tgcctatgcg
      241 ttgtttgtgc tgttttgctt ttgggctggg gcgcaattgc tgaacctgtt agtgcatgcg
      301 cccggcgtct atgagcgttt aatgcaggtc caggaaacag gtcgcccacg ggtggaaatt
      361 ggtttaggtg tcggcaccat tttcgggctg atcccgtttt tagtaggctg cctcattttt
      421 gcagtggtgg cgctatggct gcactggcga catcgccgcc agtaagattt acgttgccag
      481 atacggccta aaacgcctta tctggcatga cattcctgtt tgatcactgt ttcatacaac
      541 gcgcccgccg ttcgtctacg cgcttcgcaa accaggccgt agtcaggttg cgggtgattt
      601 tcgggctttc cagttgaatg ccaggcaaca tttcgcgggg tagtgattta cccgttttcg
      661 cttcggcaag ttgataaact ttcttgtaca gcgccgtctc ctcaaaagag aagctgtcac
      721 ctttctctaa ctgacggcga atttcgctgt cgctcatccc cagttttccc gccagtttgc
      781 gcgttgccag ttcggttttc ccgggttctt tactgtcgta acgaattaaa tcaccgtcca
      841 gtgccagctt cacgccgctg gccttactga ccgcgttctg aaacgcggca ttacggctgg
      901 cgtaccagcc agcgttaaaa tcagcaaagc ggtatatcgg tgcgctatag ctggcgggat
      961 aattcagtaa atggtaagta ccaaaccaca acccgccgcg gcgactgaag acttcctgac
     1021 gaactgtacc gtccattttc cacggatacc ctttggtatg ctgttcggca aaagcaatgc
     1081 tgacctgcat cggcccaccg gtgcgcaccg gattaagcga accaaacagc gtctgcccca
     1141 ttggtaccat gctgatcaga tcgtcaaaaa tcgcgctcaa ttgcttttct gtacgcaccg
     1201 aatctaaccg ttcgctataa cttttgccgt ttggcgattt gatttttagc gcggtatgaa
     1261 ccagaaaagc aggaatgtgc atccgttcag cacgacggtc aatctcttgc caggcgatct
     1321 tgcttaagcc cggaacggcc ggatctgcct gataatttga ctcctgctgc gccaccgcca
     1381 gcaccgagca gacgttttcc agcgtcggtg ccagcccctg actttcaaag gtggtggcga
     1441 gatctttcgc ccaggcatcg cggtctttca cgctcgcagg catcttctgc cgcacgacgc
     1501 tcgctacatc tatcgctttt tcgccctttt tcaacggttg cggagcctga ctactacacc
     1561 ccgctaaaac cagaaccgcc aacaatgaca gcgaagatag atgattgacg cgcgacatta
     1621 cttctccttt ttagctcaag gtatgggtta cttcctgaat tttgtcacca tccagctcat
     1681 gttcaaagct gcgcagacgt ttgtagatag acatcaactc aaccagtgtg gtccatgagt
     1741 taatcaggta ctggaaagca ccgcgaacct gaccaaaaac gttggtaatc tgcgtcatca
     1801 ggccgagcgt aatcgtaccg gcaacaattg acggaaacag caagaacaaa ccgaaaacgt
     1861 tatcgacctg caaatagaga atgcgggcga tgttgaaata catatagtga aaatagaggc
     1921 gaaaatagtt tttccgtacg gcgctaaaca gctcgcgtac cgtaggcggc gtcgcgcgcg
     1981 tggcatcgtc ttcaccataa accagctctt tacggtaggc ggcctctact cgctggtttt
     2041 taaactccag ccccggcagt ttgatcccta ccactgccag caatccggtc cccatcagcg
     2101 accagacgat agcggcaatc accagaccat acggaatgtg cccgataata ggcagctccg
     2161 gcacatgcgc ggagagcgtt accagcaccg gcaggaaggc gaccaacgtc atgatggcgt
     2221 tgataaaact gacgcccata ttctccagcg ttgaagcaaa acgcatggtg tcttcctgca
     2281 cacgctgtgc ggcaccttcg atatgacgca gttgttgcca gttcgccatg taatattcgt
     2341 tcatcgccgt acgccagcgg aacacatagt gactgacaaa gaagttgttc agtacactga
     2401 tcaccaccgc gatcagcgca atccccagaa agacgcccac ttcgcggtaa aattgttcga
     2461 tagtgacttt atgcggcgaa cttagcgcgg tttgaatcag atcatagaac ggtgcatacc
     2521 aggcgttgac ggcgactccg acttccacca aaaaccaggt gacgaagatg atcagtgcag
     2581 taccgagtat tgaccagtat tgccaacggt gcgggctgta gataaaccag aaaaatgcaa
     2641 aaagtcctac gcaaacaatg tagtaagcgt aaaaaatcag gaaatccaac gaccagaaac
     2701 gcgcggcgct aatcgggatc tgcccggaag cgccggtgat acgcgccacc cagtctcccc
     2761 caccggcttg ccagaagata acggcaatca atgcccaaac aaaggccgag agaaaaaacg
     2821 ctcccggctt tgggaaaaaa gacttaaaca tatgcactcc tgctaacttc ttatcgtttt
     2881 aacaactgct gtgtattaac cgcatgacca ctacaaagcg tcagccaaat gaaaccgccc
     2941 attgtgaccc atagcaaggc gcttagttcg ccagcctgac cataagaatt gtttcgacgg
     3001 atttcacaaa ccaaagattg tttacataag cggattaaca cgcgcaggat cacaaatgca
     3061 ggcattggtg agcaggaaaa agatcgtgtt tacgcgccgt tacgttttta acactattat
     3121 tgcactaatt cgccctttgc aatccatcaa tgagtagtat aaatacgctc agttaccttc
     3181 attcaatcta tggacaccac cgttgaaacg tagtctgctt ttttctgccg tgctgtgtgc
     3241 ggcgtcattg acctccgtcc atgcggcgca gccgatcact gaaccggagt ttgcctctga
     3301 tattgtcgat cgttatgccg atcatatttt ttacggcagc ggtgccacgg ggatggcgct
     3361 ggttgttatc gacggtaacc agcgcgtctt tcgcagttat ggcgaaacgc gacctggtaa
     3421 taacgttcgc ccgcagctgg attcggtcgt gcgtattgct tccctcacca agctaatgac
     3481 cagtgaaatg ctggtgaaat tgctcgacca ggggaccgtg aagttaaacg atccgctaag
     3541 taaatatgct ccgccaggcg cacgcgtgcc aacctacaat gggacgccca ttaccctagt
     3601 gaatctggca acccatacca gcgccctgcc ccgtgaacag cccggtggcg cggcacatcg
     3661 tccggtgttt gtctggccaa cgcgcgagca acgctggaaa tacctttcta cggcgaagct
     3721 gaaagccgcg ccaggcagtc aggcggcgta ctctaacctt gcgttcgacc tgctggcaga
     3781 tgcactggcg aatgcgtccg gcaagcctta tacccagttg tttgaagagc agattacccg
     3841 tccattgggg atgaaagaca ccacctacac cccctcaccg gatcagtgcc gccgtttaat
     3901 ggttgctgag cgcggtgcca gtccgtgcaa taacacgctg gcggcaattg gcagcggcgg
     3961 cgtctattcc acgcctggcg atatgatgcg ctggatgcag cagtatttat catctgattt
     4021 ttatcagcgc agtaaccagg cagatcgcat gcagacgttg atttatcagc gcgcgcagtt
     4081 taccaaagtg attggcatgg atgtccccgg caaagcggat gcgcttggtt taggctgggt
     4141 atatatggcc ccgaaagagg gtcgtccggg gattattcag aagacaggcg gtggtggcgg
     4201 tttcattacc tatatggcga tgatcccgca gaagaatatt ggcgcgtttg tggtggtcac
     4261 tcgttcgccg ttgacgcgct ttaaaaatat gagcgatggc atcaatgatc tggtgaccga
     4321 gctaagcggg aataaaccgt tggttatccc tgcatcctga ttaatactct gaaggcaaac
     4381 ggttgatagc gaccagtttg cctttattca tttcgatata gccgccttta cgcaaagcag
     4441 cgagaacttc tgcgacgaca gaacgcgata tgcgtgtacg ttgatggata taattcatca
     4501 caccaatacg tgatcgcaat tcttcattcc agtcaatcat tgataataat gttgcgcgga
     4561 tttgttcgta ggaattatgc ccaatgagct gcacgtcgcg taattccaga attctatttt
     4621 gccaggctaa ccagtaaaaa gcgtctcgcc agagttgatt ttgttcaata agcgtaatgg
     4681 tttgtttggc tggtagatga tatcccgtac aatttccttc tgatattaat ttgtatggta
     4741 tatcgttttt cattaaacca tcagccagcc ccataatata agaagcctgg gtaataccga
     4801 taagtacgtt ttcttctcta cgcagagaga taacgccctc taaaataaag aaagtatctt
     4861 cgctattgac atcactggaa aatataactt gcttttcatt attaaactcg aagcgcgtac
     4921 cgtatctgga caaacattta tcgagcttac caaattcctg aagaggttta actacagata
     4981 acatttgcgc gtcctttgca gtaatgcccg tcaaatcctt gacgggcatt atttagatta
     5041 aattaccaga atttcttcgg agtgaagaat attaccaagt atatttaaca cccacgttcg
     5101 cggaccagtc ttgatctacg tcaccaccac cgaggtagtt agcatcggta taggcgctga
     5161 agttcttggt gaagctgaac tgagtaccca gaccaacacg taccgcagac ccttcagtac
     5221 cgttatcgat ggaatcaccg ttcacatcgt tatcgttgtt tgagtcgtcg tagacgtaag
     5281 ccagtttgaa gtacggagtc agagcctgat cttcgctgta ggtgaaggta taacctgcat
     5341 ctacacccag ttcataacgc atgctgtcgt aagactgacc gtcaactttc atgtcgttgc
     5401 tcagctggta gtcatcacca gactggaaca gaccagaaac gctgccgtaa ggagtctcgt
     5461 aaccagcatc acccagtttg aagtcgtaac cggctttcaa accgaagccc cacgcgtcag
     5521 agttggtgct accgtcaacg taagtaccgt tgctcatggt tgcagacagg tcgttgttga
     5581 agtgagagta gctcaagcta ccatcaacaa agacgttgtt cgcgaagtga gcagaagagt
     5641 agatgtaggc agtctggctg tcttgatcca cctgaccaga acggtcattc atgtcacctt
     5701 tagcgaagcc tgcagccgca ccgacgatcc acttagcgtt gttaccgtca attttggtat
     5761 caacaccgac catgatgccg ttaacatcct gatcatagtt gatggtgccg ttgtcgccgt
     5821 tgaagttacc accgaagtag cttacccatg cgccgccgtt atcagccagg ccatgacgag
     5881 agttggtcag acgagtacca acggtgtctt gttccaggtt ccagatattg gtgttcgcag
     5941 acgggatgct cagcgccatg ttagcgtagt cggtcagctc catctgttgc agaacaacgg
     6001 tgttaccgcg ctgttcagcc tgataggtgt atgcacccag gtcagctttg ttagcagcag
     6061 agaaggtcgc gttggtgtta gcgtcgttta cccgaataag ttctttatct ttgtaatcag
     6121 cgatagtgcc agcgccagtt gcgttgtcga tacgaacttt gtagttaccg gttgcagcaa
     6181 caacgttatc aacagagctg ttatccagtt cagtaccgtt gtctacgtcg ccgttaccat
     6241 tgatagtcag gtgaccatca gagttcatag caacaatacc gtaaccgtag ttagacttgg
     6301 aagtatccca cgtacggtcg ttcaccagat cagcgttcag aacgtaatcg ctgctgttga
     6361 tgtcgaacac accagaaatt acgttgccat tatggtcagc aatgttgccg ctggtcagtt
     6421 ccagtttatc agtacggaaa gcttcagtat cgaactgatc aacattcaca tccagcacac
     6481 caccgttagt cacagtgata gtattggtat acagattaga ataattattc caaccggtag
     6541 attcggttaa attaacggta ctgtagctat caacagtcag gtggtctgta gcaaccacgc
     6601 catttgcacc aatattcagt gaagaaccgt tggttaaacc aatggtatct gataccagag
     6661 aggaatcagt aacatttacc tgagag
//
LOCUS       DAJVOY010000199         6640 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVOY010000199 DAJVOY010000000
VERSION     DAJVOY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.199"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..668
                     /locus_tag="OQL90_003630"
     CDS             <1..668
                     /locus_tag="OQL90_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1370259.1"
                     /translation="GFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFW
                     MTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNV
                     LSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMN
                     WQDVSGKSAANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWA
                     GQQ"
     gene            846..2099
                     /gene="avtA"
                     /locus_tag="OQL90_003631"
     CDS             846..2099
                     /gene="avtA"
                     /locus_tag="OQL90_003631"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCS1370260.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(2250..2723)
                     /locus_tag="OQL90_003632"
     CDS             complement(2250..2723)
                     /locus_tag="OQL90_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1370261.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(2825..3673)
                     /gene="yiaJ"
                     /locus_tag="OQL90_003633"
     CDS             complement(2825..3673)
                     /gene="yiaJ"
                     /locus_tag="OQL90_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCS1370262.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            3874..4872
                     /gene="yiaK"
                     /locus_tag="OQL90_003634"
     CDS             3874..4872
                     /gene="yiaK"
                     /locus_tag="OQL90_003634"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCS1370263.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            4884..5352
                     /locus_tag="OQL90_003635"
                     /pseudo
     CDS             4884..5352
                     /locus_tag="OQL90_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            5470..5943
                     /gene="yiaM"
                     /locus_tag="OQL90_003636"
     CDS             5470..5943
                     /gene="yiaM"
                     /locus_tag="OQL90_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCS1370264.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            5946..>6640
                     /locus_tag="OQL90_003637"
     CDS             5946..>6640
                     /locus_tag="OQL90_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1370265.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRS"
BASE COUNT         1600 a         1706 c         1682 g         1652 t
ORIGIN      
        1 atggttttcg ggtcgatacc gccaaacatg ttgagttgcc cgcctggcag caactgaaaa
       61 ccgaagccag ctccgcgctt cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg
      121 acaaaccttt ctggatgacc ggtgaagcct ggggccacgg cgtgatgcaa agtgactact
      181 atcgccacgg cttcgatgcg atgatcaatt tcgattatca ggagcaggcg gcgaaagcag
      241 tcgattgtct ggcgcagatg gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt
      301 tcaacgtgtt gagctacctc tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca
      361 aagcagcaga gttattacta ttagcgccag gcgcggtaca aatcttttat ggcgatgaat
      421 cctcgcgtcc gttcggtcct acaggttctg atccgctgca aggtacacgt tcggatatga
      481 actggcagga tgttagcggt aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc
      541 agttccgcgc ccgccatccc gcaattggcg cgggcaaaca aacgacactt tcgctgaagc
      601 agggctacgg ctttgttcgt gagcatggcg acgataaagt gctggtcatc tgggctgggc
      661 aacagtgact tttccggctt cccgttcatc agtacctcgg gaagccgcca accaggataa
      721 aatgtcagcc ctaatcagcg ttgcaggata aagcaccgct cactcttcaa cagaccgatt
      781 tgcaccccag caaatgtagc gttattgtta ccttccttgc tacagagttc gacagatatc
      841 ccgctatgac attctccctt tttggtgaca aatttacccg ccactccggc attacgctgc
      901 tgatggaaga tctgaacgac ggtttacgca cgcctggcgc gattatgctc ggcggcggta
      961 atccggcgca gatcccggaa atgcaggact acttccagac gctactgacc gacatgctgg
     1021 aaagtggcaa agcgactgat gcactgtgta actacgacgg tccacagggg aaaacggagc
     1081 tactcacact gcttgccgga atgctgcgcg agaagttggg ttgggatatc gaaccacaga
     1141 atattgcact aacaaacggc agccagagcg cgtttttcta cttatttaac ctgtttgccg
     1201 gacgccgtgc cgatggtcgg gtcaaaaaag tgctgttccc gctggcaccg gaatacattg
     1261 gctacgctga cgccggactg gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac
     1321 tgctgccgga aggccagttt aaataccacg tcgattttga gcatctgcat attggcgaag
     1381 aaaccgggat gatttgcgtc tcccggccga cgaatccaac aggcaatgtg attactgacg
     1441 aagagttgct gaagcttgac gcgctggcga atcaacacgg cattccgctg gtgattgata
     1501 acgcttatgg cgtcccgttc ccgggtatca tcttcagtga agcgcgcccg ctatggaatc
     1561 cgaatatcgt gctgtgcatg agtctttcca agctggggct gcctggctcc cgctgcggca
     1621 ttatcatcgc caatgaaaaa atcatcactg ccatcaccaa tatgaacggc attatcagcc
     1681 tggcacctgg cggtattggt ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc
     1741 tgcgcctgtc tgaaacagtc atcaaaccgt tttactacca gcgtgttcag gaaactatcg
     1801 ccatcattcg ccgctattta ccggaaaatc gctgcctgat tcataaaccg gaaggagcca
     1861 ttttcctctg gctatggttt aaggatttgc ccattacgac cgagcagctc tatcagcgcc
     1921 tgaaagcacg cggcgtgctg atggtgccgg ggcacaacgt cttcccggga ctggataaac
     1981 cgtggccgca tacgcatcaa tgtatgcgca tgaactacgt accagagccg gagaaaattg
     2041 aggcgggggt gaagattctg gcggaagaga tagaaagagc ctgggctgaa agtcactaaa
     2101 aattgcctga tgcgctacgc ttatcaggcc tacatgattt ctgcaatata ttgaatttgg
     2161 aagaatttgt aggccggata aggcgtttac gccgcatctg gcataaacaa agcgcactat
     2221 tccggcgttg agaaacgccg gaaaacgttt caaacaggct gctgccgtag ccgatgccgc
     2281 tgcactttct cgacatccat gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc
     2341 ggccccgtct cccgatgcca gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa
     2401 gaaacgacct ccatcgcacc aaacgggcaa gccagcatac agcttttaca gccaatgcaa
     2461 cgtgtttgtt caacgaaaat atgcccatgt tcgcggctta tcgcgtcaac agggcagaca
     2521 ttcgcgcacg gtgcatcttc acactgatga caggctactg ccgtggtcca gctgtggtct
     2581 ttaatgacac gaatacggga aataaactcg tctggtgaca acgcagcgca atcctgattc
     2641 tcctgatgcg acactgcgca agccacttca caggtacggc aaccgataca tttcgtcgca
     2701 tccgcaataa taaaccggtt catgatcact cctttgcact aaaacagcag agtgccagag
     2761 agcgcgtccc tactgctttg atccaacgaa ggaaatgtta aagttttggc acttgtccaa
     2821 agcgttatgt gatcgcgccc aggtcatccc tgacagtaaa tcccagttca ttagaaatag
     2881 cctgcgcggt ttcacgcagt ggtttcagga gatttttctc tcccacctgt ttcagacgtg
     2941 atgtcgaaag cgaaatcgac acggcgtacg gcacccgccc atgaatatca aacaccggaa
     3001 cagcaataca ggagacgccg agttcgtttt cttctctgtc catcgccgct ccgctttcac
     3061 gaatgtgcgc cagttcgtcg aacatcgcgg gcagctcggt aatggtattg cgggttaacg
     3121 gctggatctc atgctgatgg ttttcccagt atgacttcac gtagtccggg tgaccaaacg
     3181 ccatgtagat cttgcccatt gcggaacagt agagcggcat atgctggcca atataggcac
     3241 gggttcgcag catcccggtt gtgggttcca gcttataaat caaaatagcg tgatcgtctt
     3301 cgcggctgga gaagttaatg gtttcaccag tggcgatgtt cagtgcctca agatgcggag
     3361 cggcgatatg aatgatattc agcgaagaca gcgccttctg cccgacggca ataaatttgg
     3421 tagtcaggcg gtaactccct gcggcgggcg cggtggtcac atagccgcag gactgtaatc
     3481 cctgcaataa gcgatggacg gtactcttat ttaaaccagc cagctccgaa agatgcgcca
     3541 acggacaacc gtttggatag ttgctcaaaa tctcaatcag catcaacccg cgaaacagac
     3601 tctggcttcc ggctggacgc tctttttcct gcgccatctc gttctctttt tttcccatca
     3661 cttctttccc cattttgtcg cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc
     3721 tcgacagata atttataacc acttgatttt tatgtctttt gaaattcatc aatcagattg
     3781 ctttgttaaa aagtgatcga tatatttgaa atcaagtttc gcatattgaa attttaagcc
     3841 aaaaaagcga tcaaaaaaac aaggaagcct gggatgaaag tgacatttga gcagttaaaa
     3901 gcagccttta atcgggcctt aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt
     3961 gcagagatgt tcgcccgcac caccgaatcc ggcgtttatt ctcacggcgt taatcgtttc
     4021 cctcgtttca ttcaacaact ggaaaacggc gatatcattc ctgatgccca acccaaacgt
     4081 ataaccagcc tcggcgcaat tgaacagtgg gatgcccagc gttcgatcgg caacctgacg
     4141 gcgaaaaaga tgatggatcg cgccattgaa ctggctgccg atcacggtat tggtctggtg
     4201 gcacttcgta atgccaacca ctggatgcgc ggcggcagct acggctggca ggcggcggaa
     4261 aaaggctata ttggcatttg ctggaccaac tccatcgccg taatgccgcc gtggggcgca
     4321 aaagagtgtc gcattggcac taacccgctg atcgtcgcca ttccttccac gccgatcacc
     4381 atggtcgata tgtcgatgtc gatgttctct tacggcatgt tagaagttaa ccgcctggcg
     4441 ggccgtcagc tcccggtcga tggtggcttt gatgatgagg gcaatttgac caaagaacct
     4501 ggcgttatcg agaagaatcg ccgcattttg ccgatgggct actggaaagg ttctggcatg
     4561 tcgattgtgc tggatatgat cgctactctc ctttccgacg gcgcatcggt tgccgaagtc
     4621 acccaggaca acagcgacga atacggcatt tcacaaattt ttattgccat tgaagtggac
     4681 aagcttatcg acggtcccac ccgcgatgcc aagctgcaac gcatcatgga ttacgttact
     4741 accgctgaac gcgctgacga aaaccaggcc atccgcttac ccggccacga atttactacc
     4801 ctgctggccg aaaaccgccg taacggcatc actgttgatg acagcgtgtg ggccaaaatc
     4861 caggcgttat gaggagataa gtcatgattt ttggacatat cgcgcagccc aatccgtgcc
     4921 gtttgcccgc cgccattgaa aaggcgctcg attttctgcg cgctaccaac ttcaacgtcc
     4981 tggagccggg cgttgtcgaa atcgacggca agaatattta tgcgcaaatt atcgatttaa
     5041 ccacacgcga agcggtggaa aaccgtccgg aagtgcatcg tcggtatatc gatattcagt
     5101 ttctggcgtg gggtcaagag aaaatcggca ttgctattga tacaggaaat aataaagtca
     5161 gcgaatcact attagagcag cgcgatatta ttttttatca tgtcagtgaa catgaatcat
     5221 ttatagaaat gataccgggc agctacgcta ttttcttccc gcaggatgtt caccgaccag
     5281 gttgtattct gcaaacaggc ctctgagata cgaaaaattg tggttaaagt cgcattaaca
     5341 gcgctgaatt aatcaaataa tcgtctacgc cagaacgact gggtaatgtt attgctctaa
     5401 ctatattaat aatttaggtt gctgcacatg caacttgaat tatgacagac atagcctcaa
     5461 ggaatagcta tgaaaaaaat acttgaagta atactggcga ttaatctcgc cgtactttcc
     5521 tgtattgtgt ttataaacat tattttaaga tatggttttc agacaagcat tttatctgta
     5581 gatgaattgt cacgttattt atttgtctgg ttaacattta ttggcgcgat tgtggctttt
     5641 atggataacg cccacgttca ggtcaccttt ctggtggaga aactctcccc cgcatggcaa
     5701 cgacgagtcg ctctggttac ccattcctta atcttattta tttgtggcgc actggcctgg
     5761 ggcgcaacac taaaaacaat tcaggactgg agcgattatt caccgatcct cggtttgccc
     5821 atcggcctga tgtatgccgc ttgtctgccc accagcctcg ttatcgcatt ctttgaactg
     5881 cgtcatttat atcaactcat cacgcgtagc aattctctaa cgtcgccacc gcaaggagct
     5941 taatcatggc tgtgctgatt tttctgggct gtctgttggg tggtattgct atcggtttgc
     6001 ctattgcctg ggcactgttg ttgtgcgggg cggcgttaat gttctggctg gacatgtttg
     6061 atgtccagat catggcacaa acgctggtga acggagccga tagcttctcc ctgctggcga
     6121 ttccgttctt tgtgctggcg ggtgaaatca tgaatgcggg cggcttgtca aaacgcattg
     6181 ttgacctgcc gatgaaactg gtggggcata aaccaggcgg gctgggctac gtcggcgtgc
     6241 tggcagctat gattatggcg agcctttctg gttctgccgt tgcggatacc gccgccgttg
     6301 ccgctctact ggtgccgatg atgcgcagcg ccaactaccc ggtcaaccgg gcggcggggc
     6361 tgattgcctc tggcggcatt atcgcgccaa ttattccccc ttccattccg tttattatct
     6421 tcggcgtttc cagcggatta tccatcagta agctgtttat ggcgggcatt gcccctgaca
     6481 tgatgatggg cgcaacgctg atgcttacct ggtggtggca ggccagtcgc cttaatttac
     6541 cccgccagca aaaagcaacg atgcaggaaa tctggcactc ttttgtctcc ggtatctggg
     6601 ctctgtttct tccagtcatc attattggcg ggtttcgctc
//
LOCUS       DAJVOY010000200         6455 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVOY010000200 DAJVOY010000000
VERSION     DAJVOY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.200"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..357
                     /locus_tag="OQL90_003638"
     CDS             <1..357
                     /locus_tag="OQL90_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370266.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPLIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILFFSPDHFNNDFY"
     gene            379..1557
                     /locus_tag="OQL90_003639"
                     /pseudo
     CDS             379..1557
                     /locus_tag="OQL90_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(1945..2321)
                     /gene="rnpB"
                     /locus_tag="OQL90_003640"
     ncRNA           complement(1945..2321)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQL90_003640"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2354..3499)
                     /gene="garK"
                     /locus_tag="OQL90_003641"
     CDS             complement(2354..3499)
                     /gene="garK"
                     /locus_tag="OQL90_003641"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS1370267.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTVL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3596..4486)
                     /gene="garR"
                     /locus_tag="OQL90_003642"
     CDS             complement(3596..4486)
                     /gene="garR"
                     /locus_tag="OQL90_003642"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1370268.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4516..5286)
                     /gene="garL"
                     /locus_tag="OQL90_003643"
     CDS             complement(4516..5286)
                     /gene="garL"
                     /locus_tag="OQL90_003643"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCS1370269.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(5302..>6455)
                     /gene="garP"
                     /locus_tag="OQL90_003644"
     CDS             complement(5302..>6455)
                     /gene="garP"
                     /locus_tag="OQL90_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1370270.1"
                     /translation="VPKSYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDM
                     FPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLAL
                     FSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIYNPTDHPRMSAEELKFISENGA
                     VVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQ
                     EKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLAST
                     IILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVAS
                     IVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1758 a         1685 c         1409 g         1603 t
ORIGIN      
        1 gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa
       61 ccggaaaata tgagtccatt aatcatagat atttctacag gagaacatga taattatatc
      121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
      181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
      241 acccaaaaag aagccatctt aacagcccct acgcacttta ataaaacctg cattacttca
      301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
      361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
      421 ttagtccgtc gaaaaatgaa tgaccttcct gaaatcccca tagaaaataa tctcgaagca
      481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
      541 gcgttacaac caggtggaaa atgtattatc actggtgatt ttcaaaaaga attagcgtac
      601 ttacaaaatg taattcttta taatgactcg tttcttcgtc tggatttttt aggttataac
      661 gcccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
      721 aaccaggaaa tatctacagg caatatcaat gttaattgtc cattaaaaga tatttacaat
      781 gaaatcagga ggttgaacgc aacctttagt tgtgggactg gagatatagt tgatctatcc
      841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
      901 aatactattc taaatccttt taaattggat tcaacaaatt ttactaatgc caacatgttt
      961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
     1021 ataacgcctg ttttaataag tattagcgat atgtattctg aagaaaaaat caagtttgtc
     1081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
     1141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
     1201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
     1261 gagatgaaag aattctcatc aaaaaaaaac atatgattac atcgatataa tcattgaaga
     1321 ttatgaaaat atcaaagaaa atgcttatct ggtcgtcccc caaattaatt atactatgga
     1381 tttaaacata gaagactcca gctcagaaga gttaataccc tcgagaaaga cgaaaattct
     1441 tcggacaatg gctttgaagt cgaagaatat aacgcatatg aagcatataa ctcagagaat
     1501 ctatatttta ccagagagga atatacgtat gattacgacc tttcaaatgc aatatagact
     1561 taataacttc ttacaaaatc tataattaat accgtagata ttcattcatc ttaccctgtg
     1621 caccaaaagc aaaaactcac tgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct
     1681 gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt
     1741 gtaggcagga taaggcgttc acgccgcatc cggcatgaat aaagcgcact caacaatctg
     1801 aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa gccgctttct
     1861 tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg
     1921 ccgaagcggg tttttacgta aatcaggtga aactgaccga taagccgggt tctgtcgtgg
     1981 acagtcattc atctaggcca gcaatcgctc actggctcaa gcagcctacc cgggttcagt
     2041 acgggccgta ccttatgaac ccctatttgg ccttgctccg ggtggagttt accgtgccac
     2101 ggactgttac cagccgcgcg gtgcgctctt accgcaccct ttcaccctta cctgatcccg
     2161 cttgcgcggg ccatcggcgg tttgctctct gttgcactgg tcgtgggttt cccccccagg
     2221 cgttacctgg caccctgccc tatggagccc ggactttcct cccctccgcc cgtctccccc
     2281 gaagaggacg acgacgaagc ggcgactgtc tggtcagctt cggcgcgcag tatagagggt
     2341 ttgcgcgccc ttgtcacccc gcgttgcgca ttccaatcgc cagtgtcgcg gcgatattac
     2401 gtgaagcacg gcagatattg tcataagccc cgcggaatgc ttcgtccaac gtacctatgc
     2461 tggtcaatac gctgaagacc gcatcaatgc catgctgatg tacaacgcca acatcatcgg
     2521 tcaggctacc cgcaatgcca atcaccggtt tatggtactt cttcgccacg tttgcgacac
     2581 caatcggtac cttgccgtga atactctggc tgtcaatacg cccttcaccg gtgatcacca
     2641 gcgtacaatc gtgaatatgt tcttccagat tcagcaccgt agtgacgatt tcaataccac
     2701 ttttcagttc cgcaccaaga aacgccatca gcgccgcgcc cataccgccc gcagctcctg
     2761 cgccggggac atctttcaca tcaacatgca gcgctttttt aatgacctcg gcatagtgag
     2821 agaggttatt gtccagctca acaatcatcg cttcactggc tcccttttgt gggccaaaga
     2881 tgcgcgatgc gccgttatcg cccaccagcg gattggtgac atcacaagcg acgcgaatga
     2941 cgcaatcttt taagcgcgga tcgaggccgg aaatatcaat atcattcaga gtattaagac
     3001 taccgccgcc aaaaccaatt tcattgccgt tggcgtcgca taatttcgcc cccagcgcct
     3061 gtaccatgcc tgcgccgcca tcatttgtag cgctgccgcc aatgccgata ataatgtttg
     3121 tcgcaccgct ctccagcgcc tgcaggatta actcgcctgt gccgcgtgaa gtggtcacga
     3181 gtggatcgcg tttttccgca ggtaccagct ccagcccact ggccgccgcc atttcaataa
     3241 acgcggtttt gccatcgccg gagatccccc aactggcatt cactttctcg cccaacggcc
     3301 ctgtaaccca ggcgtgacgt tcggaaccct gggtggctgc aatcatcgct tccaccgttc
     3361 cttcgccacc gtcggcaacc ggaacagaaa cgtactgtgc atcaggaaaa atttcccgaa
     3421 atcctttttc tatcgcctgc gcaacctcgc tggcagataa actttcttta taagagtctg
     3481 gggcgattac gattttcata cctatgcctg ttaccacatg acgccggagg gcgtttctct
     3541 tattcggcgt ggattccagg cccggattgc aatacgccat ccgggcacga cgtcattaac
     3601 gagtaacttc gactttcgcc agtttttcgt agtagcacgc cagagcgcta tgatccgccg
     3661 ttcctaaacc atctgcacgc agtgcctgca tcatctccat aaccgcagct gtgagcggca
     3721 gttgcgcgcc gacgccgtga gaagtatcca gcgcattcgc cagatcctta atatgcagat
     3781 caatgcggaa gcccggcttg aagttgcggt ccatcaccat cggcgctttg gcatccagca
     3841 cggtactgcc cgccagccca ccgcgaattg cctgataaac caggtccggg ttaacgcccg
     3901 ctttagttgc cagcgttaac gcttctgaca tcgcagcaat attcagcgcc acaatgacct
     3961 gatttgccag tttggtgacg ttacctgcac cgatttcccc ggtatgcacc acggaacccg
     4021 ccatcgcttt catcaaatca tagtatttgt cgaaaatagc cttgtcgccg cccaccatca
     4081 ctgacagcgt accgtcgatg gctttcggtt caccgccgct caccggagca tccagcatat
     4141 caatgccttt cgctttcagc gcttcgctga tttcacggct tgccagcggt gcgatagaac
     4201 tcatatcgat caataccgta cctggcttcg cgccttcaat aatgccattc tcacccagcg
     4261 ccacctcttt cacatgaggg gagtttggca gcatggttat gatgacgtcg cactgttcag
     4321 cgatcgcttt agccgtagac gctgtttctg cacctgcagc aatcacgtca gcaatagctt
     4381 ctgggttacg gtcagcaacc accagcgagt aacctgcttt cagaaggttt ttactcattg
     4441 gtttacccat aatccccagg ccaataaaac caactttcat agtcatatca atcatctctc
     4501 ttgttgcggt ggtggttatt ttttaaaggt atcagccagt ttctgagtgg cagagcggaa
     4561 gacgccaaga tcgctgccga cagccacaaa cgtcgcgccc cattccagat aacgacgcgc
     4621 atcggcttcg accggcgcga gaataccgct gggtttgccg tgcgcactgg cacggttaaa
     4681 aatatgctga attgcttttt gtacatccgg atgcgaggca ttgccgagat ggcccaatgc
     4741 cgcagccaga tcgctggggc cgacgaagat accgtctaca ccttcggtag cggcaatagc
     4801 atcgacgtta tctacgccct gctgactttc tatctggacc agaatagtga tgttcttgtt
     4861 cgactgagcg aaataatccg ccacggtgcc aaacatattg gcgcggtgag aaacggagac
     4921 gccgcgaatg ccttccggcg ggtaacgagt tgatgccacc gcctgctctg cttcctcttt
     4981 tgtttctaca aaaggaatca ggaagttata gaaaccgata tccagaagac gcttaataat
     5041 taccggctcg ttggtcggca ctcgcactac tggcgcgctg gcgctgcctt tcagggccat
     5101 taactgcgga ataaacgtgg aaatatcgtt tggcgcatgt tcgccatcca gcaccagcca
     5161 gtcaaaccca gccaaaccaa gaacttcagt gctaatcggg ttagagagtg ctgaccagca
     5221 accaatttgt acctgtttcg cagccagtgc ggctttgaat ttattcggga aaacatcgtt
     5281 attcatcgct tatacctttg cttatttctg caattccata cgtttaatgt cgccaactac
     5341 gaagaggtag cagaccatcg ccatcagcgc tgaacatccc acgaaaacca gtgctgcatt
     5401 gaaggagtgc agttcactta ccaggtagcc aatcaccagt ggagtgacaa tggaggcaac
     5461 attgccaaag acgttaaaga cgccgccaca gaggccaaca atctctttcg gcgcggtgtc
     5521 agaaatcacc ggccagccca gagcaccaaa tcctttgcca aaaaaagcca gcgccatcag
     5581 catgaccacc agcgtggtgt tgttggtgta gttacataag atgatggtgg aagccagcaa
     5641 cattcccagc acaatcggta gcttacgtgc cagggtcagg gataaaccgc gtttgatcag
     5701 ataatccgag aagacacctc ccagcacgcc gcccgcaaaa ccacacagtg ccggaatcga
     5761 ggcgaccaga cccactttca gaatcgacat gcctttttcc tgcaccagat aaatcgggaa
     5821 ccaggtgagg aagaaccagg tgatggtgtt gataaaatat tgtccgaaaa atacgcccaa
     5881 catcatgcgg ttagagagca attgcttgat gtaatgcagt ttgggaccgc ttgctgccgc
     5941 gctgcccggc tttttgtggt ccatatcgac caccgcgcca ttttcagaga taaacttcag
     6001 ctcttccgca gacatacgtg ggtgatctgt cgggttataa atcaacttga tccacagcgc
     6061 cgtcagcaca aaaccaatca cccccataac ggtgaagacg tgctcccagc cccaggcgaa
     6121 agtcagccag ccaagcagag gcgaaaagag cgccagcgag aaatattgcg ccgagttaaa
     6181 gatggcggag gcagtaccac gttctttcgt cgggaaccag gcggcgacaa tacgggcgtt
     6241 cgccgggaat gatggcgctt ccgagaagcc gagcataaag cgcataaaga acatagagat
     6301 ccctgcccag gccagcggga acatatcaac aaagccttgc aggaaggtga atagcgacca
     6361 gaaaaagagg ctgtaggtgt aaactttttt cgagccaaac ttatcaagca gccagccgcc
     6421 gggaatttgc atcagcaggt aggacttcgg tacca
//
LOCUS       DAJVOY010000201         6384 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVOY010000201 DAJVOY010000000
VERSION     DAJVOY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.201"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..209)
                     /locus_tag="OQL90_003645"
     CDS             complement(<1..209)
                     /locus_tag="OQL90_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCS1370271.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNS"
     gene            688..2466
                     /locus_tag="OQL90_003646"
     CDS             688..2466
                     /locus_tag="OQL90_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCS1370272.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(2506..2985)
                     /gene="xdhC"
                     /locus_tag="OQL90_003647"
     CDS             complement(2506..2985)
                     /gene="xdhC"
                     /locus_tag="OQL90_003647"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS1370273.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2982..3860)
                     /gene="xdhB"
                     /locus_tag="OQL90_003648"
     CDS             complement(2982..3860)
                     /gene="xdhB"
                     /locus_tag="OQL90_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1370274.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3871..6168)
                     /gene="xdhA"
                     /locus_tag="OQL90_003649"
     CDS             complement(3871..6168)
                     /gene="xdhA"
                     /locus_tag="OQL90_003649"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1370275.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
BASE COUNT         1592 a         1508 c         1614 g         1670 t
ORIGIN      
        1 gagttgtcgc ggaatacgga aatacctaat ccactattaa agactttggt tgagatgttt
       61 tcagcacgca gtgcttttaa tgctgcggca acgtccagta cttgtttcag ttcatctggc
      121 gtctgttccc acgttaacaa aaaatctttc tcgtgaaggt gagaggtcag cgaattgata
      181 tccttaatca gctcattaac agttttcatc ataaagtccc ggtagtggaa tgacaggcaa
      241 agtggtttat ttcactgtga cttgccaaag tgattggtgt tttcacagct atacctggga
      301 ctaacaataa gtgtgccaga attatccgcg cggctgcaaa agactaattt gacggataat
      361 gtgtggggtt gatcacaaat tgatgccagc ttctggttgt ttgtaaaata cgaaaacgtt
      421 tggctttttt tatcgcgtta tttatttgct gctaagtatc agaaataaga taaaggggca
      481 ggctatctct cttcagtttg ataaactcac cgccgtaatt ctcatattga tatagaaatg
      541 tgatatctct tccataattt cattagaaaa tgataaaaat cttatgtttt tatcaaagcc
      601 aatttcgcct gagttaatat catcatgatc tgtgaaccat caacgtcttc aatgatcgac
      661 ttttaagtaa gtctggaagg taaagctatg gagcttgcta ctacgcagtc agtattgatg
      721 caaattcaac cgacaattca gcgttttgcc agaatgcttg ccagcgtttt gcagcttgag
      781 gttgagatcg ttgatgaaaa cttgtgtcgc gtcgccggaa cgggcgcgta tgggaagttt
      841 cttggtcgcc agttgagcgg caactcacgc ctgctccgcc acgtcctgga aacgaaaact
      901 gaaaaagttg tgacacagtc tcgcttcgat cccctttgcg aaggttgcga tagtaaagaa
      961 aattgccgtg aaaaagcatt tctgggtacg cctgtcattt tacaggatcg ttgtgttggg
     1021 gtgataagtt tgattgccgt tacccacgag caacaagagc atatcagtga taatttacgc
     1081 gaattttctg attatgttcg ccatatatcc accatttttg tttcgaaact tctggaggat
     1141 caggggccag gagataacat cagtaaaata ttcgcgacca tgatcgataa tatggatcag
     1201 ggcgtattag ttgttgatga tgaaagtcgg gttcagtttg ttaatcagac tgccttaaaa
     1261 acacttggtg ttgtacaaaa taatattatt gggaaaccta tccgtttcag accattaaca
     1321 tttgagagta attttactca tggacatatg cagcatattg tttcgtggga cgataaaagt
     1381 gaattaatca ttggtcaatt gcataacatt cagggccgac aattattttt aatggcattt
     1441 caccaatcgc ataccagttt ttctgtagca aatgcacctg atgaaccaca tattgaacaa
     1501 ttggttggcg agtgccgtgt tatgcggcaa ttaaaacgac tcattagccg tattgcaccc
     1561 agcccatcca gcgttatggt ggttggtgaa agcggcacgg gtaaagaagt cgtcgcccga
     1621 gcaatccata agttgagcgg aagacggaat aaacccttta ttgctatcaa ctgtgccgcg
     1681 attccggagc agcttctgga aagcgaactg ttcggttatg ttaaaggcgc atttactggc
     1741 gcttctgcca acggtaaaac agggttgatt caggcggcga atacgggcac gctgtttctc
     1801 gatgaaatag gtgatatgcc attaatgttg caggctaaat tactgcgcgc tattgaggcg
     1861 cgtgaaattc tgccgattgg tgccagtagc ccaatacaag tcgacattcg catcatttct
     1921 gcaactaatc agaatttggc ccagttcatt gccgaaggta aattccgcga agatctcttc
     1981 taccgactta atgttatccc gataactctg ccaccgctgc gtgaacgtca ggaagatatt
     2041 gaactattgg tgcattactt tttacatctg catacccgtc gtctgggatc ggtttatcct
     2101 ggcattgctc ccgatgtcgt cgaaatattg cgtaagcatc gttggcccgg aaacctgcgc
     2161 gagttaagca atttgatgga atatctggtt aacgtagttc cttcaggtga agttatcgac
     2221 agcacgctat tgccgccaaa tctgctgaat aatggcacaa cggagcaaag tgatgtaaca
     2281 gaggtcagtg aggcgcacct gtcactcgat gatgcgggcg gcacggcgct ggaggagatg
     2341 gaaaagcaaa tgatccgcga ggcgctttca cgtcataaca gcaagaagca agttgctgat
     2401 gaactgggca tcggcattgc tacgctctat cgcaagatta agaaatatga gttgttaaac
     2461 acataacctc agtgaatccg gcctgaattc aggccggata tccttttact tcgttttctc
     2521 gcaatccaga actgtattta caatcatctg ataccccgtg cagcgacaaa gatttcccgc
     2581 cagtccgcga cgaatttccg taatggttaa tggcttctcg cgtggtttcg ccagcattgc
     2641 cgtggtagcc ataatcaggc caggcgtaca aaacccgcac tgcactgcgc cggatttcgc
     2701 ataagcctgc tgaacatgag aaagttttcc gcctttcgct tcaccttcca gcgtgcggat
     2761 ctcttttcct tcagcccagg cggcaaggta taagcaactg tctattgctg tgccgtcgac
     2821 caacaccgta caggcaccac attcacccac gcagcaccct tgtttgacac ttagcagtcc
     2881 ttgttcgcgg agtaattccg agagcggcgt gcctggtgcg gcgtgaagct gaaaaggcat
     2941 cccgttaatg gtgcattcga tggtaattgt ttcgctgtga ttcattgcaa ttttcccccc
     3001 gccgcggcga cggcttcgct aatcactttt ttggtcatcg tctggatgag atgcagacga
     3061 aactctttac tggcccgcca tgaagaacgc ggggcgacat cttgcaggac agattcgctg
     3121 atagcttcca gcgtttgcag gtttaatggc gcattttgtg cagtctgttc ggcatgttgg
     3181 cagcgaatcg gcgttggcgc ggcaacacca aatgccaggc gtaattcgct gaaattgccg
     3241 ttatccagtc ggcaatgtgc ggcgcagcca atcgttgaaa tatccattgc gtcgcgcatg
     3301 gcatatttaa aatgcgcgct gcccgcgtgt tctttcggct gtggcggaaa atgaaaggcg
     3361 acgaggattt cgtcatgctc aagagacact ttgcccggcc cggtgtgaaa gccattaatc
     3421 gggacgaaac gaacaccgcg cggggagtgg atctccagtt tcgcgtcata aattagcgtt
     3481 ggcgtggcag aatctgcgct ggtggcaccg ttgcaaatat ttccaccgta ggtagcgacg
     3541 ttacggatct gcagtccagc aatggacatg gccgcagcac ataacgccgg gagatgacgt
     3601 tgagttatag gatcttctat tagctgggta aatgtcgttg cagagccgat acgtagcgag
     3661 ccatcttccg ccagcgtaat tccccgcagc tccgccagat tatgaatatc aacaatatgg
     3721 cgataacggt cattgtgatg gtggagctga atcagtacgt cagtgccacc ggcgagcagt
     3781 ttggcctgcg ggttgttagc cagcaggttg atggcatcgg caagggttgc tgcgcgatgg
     3841 taagaagcaa aatcaaacat gatgttatcc tcaaatcaat cctgccagat ggaactcttc
     3901 atataaccgt tttggcgtca gcggcagtgt attgattgca acaccggtag ccatcttcac
     3961 cgcgttacga atagcagcgg caacaggaat tattggtggc tcacccagtg acttatgtcc
     4021 gtatgcggat tgcggctcat tgatttcgac gaacgcgctt tccagttgtg gcagatccgg
     4081 catggtcggc attttgtaat ccagcagatt ggggttacgg accacgccgc ttttagcatc
     4141 gatgatcatc tcttcaaata gcgcccagcc aatgcccatt cccattccgc cgtgtacctg
     4201 accttctgcc agcagtggat taagaatatg ccctgaatca tgaacgttga ggatgcggtt
     4261 gatggtgact ttgcacagcg caatatcgac cgtcagatca acaaaggtac agccaaacgc
     4321 cggtgggtta gtggtggttt tgatggagct ttcagcagag agctgcccgc cgcgttcagg
     4381 gtggtagaaa gcgtccatcg ccaaatcttt taacgacatt aacggctctt ccggtcgttc
     4441 aaccagcacg atatggcctt ttatcagggt cagattcatc gctgactgat gtagcatgac
     4501 tgcggcgtga gcgatgattt tctcttttaa taatagtgcc gcactgcgca gcgcaggcgc
     4561 ggcaacatag ctctggcgtg aggcaaatgt gccgggatcg aacggcgtaa cgtcggtatc
     4621 ttgagttgaa ataacgcgaa cgtcgctgac cggaaccccc acggtttctg ccaccatttg
     4681 cgagaagacg gtgtcggcac cctgaccgat ttccgtcgcg ccgctttgca cgttgatggt
     4741 tccatcctga ttcatcagaa ggcgcgcgcc tgctatttct acgccgacag gccaggtgtt
     4801 agaggtgtag ctaaaacagg cgacgccaac gccgcggcgc aaattgcctt gctggttctg
     4861 gcattctgca cggcgttttt cccattcaaa gattttccgg cctttttcaa gacactccgg
     4921 caaccctgcg ctgtaaatac gtttgcccgt gagcggatta gcatctcctt cgcgggcggc
     4981 gttgcgtaaa cgaatttcaa caggatcaat acctaacgct gtcgcggcgt catcaagcat
     5041 agactcaacg gcaaatacga cttgtggcgc gccataacca cgcatcgcac cagccgaggg
     5101 gaggttggta tagcaggtct ttgaactgta agcgtaggca caacgaggat aaaggtaagc
     5161 gactttattc ccccccgcag aagcgatgga gtgcccgtga gatgcataag cgccggtgtt
     5221 agacagaaca tccagactat aacctttcaa tgttccgtcg cggttcacgc ccatttgccc
     5281 gtcaatggta aaagcgtggc gggtacgggt tgcgaggaaa cactcttcac ggctaaggga
     5341 aactttcacc ggaatgccgc caagcttgct ggtcaggaat gccgccattg gctcttccag
     5401 tacatcctgt ttattaccaa aaccgccacc gacaaatggt ttgatgactc gtacgcatga
     5461 ccagggaata tccagcgcct gaccaaccac gcggcgaaca atgtgcggga tctgggtgct
     5521 ggaaacgatg gtaattcgcg agtcatcctc catccacgcc agcgatgtta cgctttccat
     5581 gtgacaatgt tgaataacgg gggtctgata gtgcccctgt acctggtagt cggcggcatc
     5641 gattgtttgt tggacattac ccgtcgacat cgtgctttgt ttcagtaaat tgccaccgtt
     5701 atggattggt gcagcgtctt ctgccagcgc cgcttctggc gtggtgataa cgggtaattc
     5761 ttgccactca atgctgacca attgcgccgc tttttctgcc gtgagttcat cgcgggccac
     5821 gacgatggca atggcgtcac catgatgacg aacatggcga gttagcagtg cgcgatcggc
     5881 ggtatcgcgc ttgttttcgt caagtgtcca ggcatgccca gctgtagcga atggaatatc
     5941 aggcacatct tcccaggtaa aaatcgccag tacgcccggc aaacttctgg cttgttcatc
     6001 attaatactt acggcataac catgtgcgat agggctacgt acatatttcg cataacacat
     6061 gcccgccata acataatcgt cagtatatcg tgcccgcccg gtgaccttag caatggcatc
     6121 gacgcgcatg catgattcac ccgtagcggt tgcttcccgc gcttccatag aaatcccctc
     6181 gatttgaaca tttggttctt atggcaaaga tttagtctaa gcaggcaaga tttacgccag
     6241 aaacgacgat aagcgttagg ggaggtagga agtgtgatga cgttaaaaaa acacagatat
     6301 gatacgaaac aggagtctga ataggggcag tgatacgtat caatatgagt ggcgataaaa
     6361 tcaatgtgat aaattgggat tatt
//
LOCUS       DAJVOY010000202         6335 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVOY010000202 DAJVOY010000000
VERSION     DAJVOY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.202"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            168..1013
                     /locus_tag="OQL90_003650"
     CDS             168..1013
                     /locus_tag="OQL90_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1370276.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLSN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            1036..2547
                     /locus_tag="OQL90_003651"
     CDS             1036..2547
                     /locus_tag="OQL90_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1370277.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGGRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2572..3570
                     /locus_tag="OQL90_003652"
     CDS             2572..3570
                     /locus_tag="OQL90_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1370278.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3603..4697
                     /locus_tag="OQL90_003653"
     CDS             3603..4697
                     /locus_tag="OQL90_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1370279.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4709..5581
                     /locus_tag="OQL90_003654"
     CDS             4709..5581
                     /locus_tag="OQL90_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1370280.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5629..6051)
                     /locus_tag="OQL90_003655"
     CDS             complement(5629..6051)
                     /locus_tag="OQL90_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1370281.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6148..>6335)
                     /locus_tag="OQL90_003656"
     CDS             complement(6148..>6335)
                     /locus_tag="OQL90_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HCS1370282.1"
                     /translation="LCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGK
                     TFDAKLLIDENIALKSL"
BASE COUNT         1466 a         1515 c         1835 g         1519 t
ORIGIN      
        1 ttgttaataa aaaccctaca ggaggcacta tgcctaaaaa aatgagaaca acccgtaatc
       61 tattattaat ggctacgcta ttaggtagcg cactatttgc cagggctgcg gataaagaaa
      121 tgaccattgg tgcaatatac cttgatacct agggatatta cgctggagtg cgtcagggcg
      181 ttcaggatgc ggcaaaagat tcttcagtac aggtacagtt aattgaaact aacgcccagg
      241 gtgatatttc gaaagaaagt acctttgttg ataccctcgt ggcgcgtaat gtcgatgcca
      301 ttattttatc ggcagtgtct gaaaatggca gtagccgtac cgttcgtcgc gccagtgaag
      361 cgggtattcc ggtgatttgc tacaacacct gtattaatca aaagggtgtc gataaatatg
      421 tctcggcgta tctggtcggc gatccactgg aatttggtaa aaaactgagt aacgctgccg
      481 ccgattattt tattgccaat aaaattgacc agccgaaaat tgccgtcatc aattgcgaag
      541 cctttgaagt ttgtgtgcag cgacgtaaag gatttgaaga agtattaaaa tcccgcgttc
      601 ccggcgcgca aattgtcgct aatcaggaag ggactgtttt agataaagcg atttccgttg
      661 gtgaaaaact gattatctcc acgccggatc tcaacgccat tatgggggag tcgggcggtg
      721 cgacactcgg cgcggtaaaa gcggtacgta atcaaaatca ggccggaaaa attgctgttt
      781 tcggttcgga tatgacaacc gaaattgctc aggagctgga aaacaatcag gtgctgaaag
      841 cggtagtgga tatttccggt aagaaaatgg gcaatgctgt tttcgcgcaa acattgaagg
      901 ttatcaataa acaagccgac ggtgaaaaag tgattcaggt gccaatcgat ctctatacca
      961 aaacggaaga tggtaaacag tggctggcaa cgcacgttga tggtctgccc taatcgctga
     1021 ttcgcagagg tccctatgtt cacggcaaca gaggcagtcc cggtagcaaa agtggtggca
     1081 ggaaataagc gttaccccgg cgtcgttgcg ctggataacg ttaacttcac gctcaataaa
     1141 ggcgaagttc gtgcgctgtt aggtaaaaac ggcgcgggca aatcgactct cattcgaatg
     1201 cttaccggca gcgaacgccc ggatagcggt gatatctgga ttggcgagac gcgactggaa
     1261 ggtgacgaag ctacgttgac tcgccgtgcc gctgaactgg ggggtcgtgc ggtttatcag
     1321 gaattaagtc tggtggaagg gctgacggtg gcagaaaacc tctgccttgg tcaatggcct
     1381 cgccgcaacg gcatgattga ttacctgcaa atggcgcagg atgcccaacg ttgcttacag
     1441 gcgctgggcg ttgacgttag tcctgaacaa cttgtttcaa cgctaagccc ggcgcaaaag
     1501 caactggtgg aaattgcacg ggtgatgaag ggcgagccgc gcgtggtcat tcttgatgaa
     1561 cccaccagct cgcttgccag tgcagaagtt gaactggtga tcagtgcggt gaaaaagatg
     1621 tcggcactgg gcgtggcggt gatttatgtc agccaccgga tggaagaaat ccgccgcatt
     1681 gcctcctgtg ccaccgttat gcgcgatggt caggtggcgg gcgatgtgat gctcgaaaac
     1741 acctccacgc atcatattgt gtcgctaatg ctagggcgtg atcacgttga tattgcgccg
     1801 gttgcccctc aggaaattgt ggatcaggcc gtactggaag tccgtgcgtt acgccataag
     1861 cccaagctgg aggatatcag ctttacgtta cgtcgcggcg aagtgcttgg cattgctggc
     1921 ctgctggggg cagggcgcag tgaattgctg aaggcgattg ttgggctgga gacgtatgaa
     1981 cagggcgaaa ttgttatcaa cggcgagaaa atcacgcgcc ccgattacgg cgacatgctg
     2041 aaacgcggca ttggctatac gccagaaaac cgcaaagaag cggggatcat tccctggttg
     2101 ggcgttgacg aaaatacagt gctgaccaat cggcaaaaaa tcagcgccaa cggagtgctg
     2161 caatggtcca ccatccgccg cctgaccgaa gaggtgatgc agcggatgac ggtcaaggcc
     2221 gccagtagcg aaacgcccat cggcacgctt tctggtggca atcagcaaaa agtggtgatc
     2281 ggtcgttggg tctatgccgc cagccagatt ttgttgctcg acgagccaac gcgcggcgtc
     2341 gatatcgaag ccaaacagca gatttaccgt attgtccgtg agctggctgc cgaaggaaaa
     2401 agcgtggtgt ttatctccag tgaagtggag gagttgccgc tggtgtgcga ccgcatcctg
     2461 ttgttacagc acggtacgtt ctcgcaggag tttcactcgc cggtcaatgt ggatgagctg
     2521 atgtccgcca ttctgtctgt gcactgatga attttaccga ggaacctgaa gatgtctgct
     2581 tcgtcattat cattgccgct gggcaagagc gtctcgctca aacaatttgt cagtcgccat
     2641 attaatgaga tcggtttgct ggtggtaatc gccattcttt atctggtctt ctccctgaat
     2701 gcgcctggct ttatctcatt gaataaccag atgaacgtgc ttcgcgatgc tgccaccatc
     2761 gggatcgccg cctgggcaat gacgctgatt attatctccg gtgaaattga tgtcagcgtt
     2821 ggaccgatgg tggcttttgt ctcggtgtgc ctggcatttt tgctgcaatt tgaagttccg
     2881 ttggcgattg cttgtctgct ggtgttgctg ttaggcgcgc tgatgggaac gctcgccggg
     2941 gtgctgcgcg gcgtgtttaa cgtgcccagt ttcgttgcca cactggggct gtggagcgcc
     3001 ctgcgcggaa tggggctgtt tatgaccaac gcactgccag tgccgattga cgaaaacgag
     3061 gtgctggact ggctgggcgg acaatttctc ggtgtgccag tatccgcgct gatcatgatg
     3121 gtgttgtttg cgctgtttgt gttcattagc cgaaaaaccg ccttcgggcg ctcggttttt
     3181 gccgttggcg gtaatgccac ggcggcgcag ttgtgcggta ttaacgttcg tcgagtgcgc
     3241 attcttatct ttaccctttc gggattatta gcggcggtga ccggcatttt gctggcggca
     3301 cgcctcggtt ccggtaacgc aggtgccgca aacggtctgg agtttgacgt catcgccgcg
     3361 gtcgtggtcg gcggtacggc actttccggc ggtcgcggtt ccctgttcgg tacattgctt
     3421 ggtgtgctgg tgattacgct aatcggtaac ggtctggtgc tgctcggtat taactccttt
     3481 ttccagcagg tggtacgcgg cgtcatcatc gtggtggcgg tgctggcgaa tatcttgctg
     3541 acccagcgaa gcagtaaagc gaaacgctaa accttaacgc ccctggcgcg ggtgcggcag
     3601 ggatgaaaat taatcgtatt gttaatgagg gatttatgaa aacgatgctg gcagcttatt
     3661 taccaggaaa ttcaaccgtc gatctgcggg aagttgcggt gccgacgccg gggattaacc
     3721 aggtactgat caaaatgaaa tcctccggga tttgcggaag cgatgtccac tatatctatc
     3781 atcaacaccg tgccacggcg gcggcacccg ataaaccgtt ataccaggga tttatcaacg
     3841 gtcatgaacc atgcgggcag attgtggcga tggggcaagg ctgtcgccat tttaaagagg
     3901 gtgatcgcgt gctggtgtat cacatttctg gctgtggttt ttgcccgaac tgccgccgcg
     3961 gctttcctat ctcttgtact ggcaaaggaa aagcggctta cggctggcag cgtgacggcg
     4021 gtcatgccga atacttgctg gcggaagaaa aagatctgat cctcctgccg gatgcgctta
     4081 gctacgaaga tggtgcgttt atcagttgcg gcgttggtac agcgtatgaa ggaattttgc
     4141 gcggcgaagt ttccggcagt gataacgtgc tggtggtcgg tctggggcca gtcggcatga
     4201 tggcgatgat gctggcgaaa ggtcgcggtg caaaacggat catcggcgtt gatatgctgc
     4261 cggaacgtct ggcgatggca aaacagttag gggtgatgga tcacggctat ttagcaacca
     4321 ccgaaggtct gccgcagatt atcgccgaac tcacccacgg tggcgcggat gttgcgctcg
     4381 attgttctgg taatgccgca ggtcgcttgc tggcactgcg atccaccgct gattggggac
     4441 gggtggttta catcggagaa accggaaaag tggagttcga ggtcagcgcc gatctgatgc
     4501 accatcaacg gcggattatc ggctcctggg tgaccagtct gttccatatg gaaaaatgcg
     4561 cccatgatct gacggactgg aaactgtggc cgcgtaacgc cattacccat cgcttctcgc
     4621 tggaacaggc tggagatgcc tatgcgctga tggcgagcgg caaatgcggg aaagttgtga
     4681 ttaacttccc ggattaagga gttagcggat gaccatctat accttatctc acggaccgct
     4741 aaagctggat gtctctgacc agggcggcgt aatcgaaggc ttctggcgtg acacgacgcc
     4801 gcttttacgt cctggtaaaa aaagcggtgt ggcaaccgat gcttcctgtt ttccgctggt
     4861 gccgtttgca aaccgggtga gcggcaatcg ttttgtctgg caggggcgtg agtatcaact
     4921 gcaaccgaat gttgagtggg atgcgcacta tctacacggc gatggctggc tgggcgagtg
     4981 gcaatgtgtc tcgcacagcg atgatagctt gtgtctggtg tatgaacatc gcagcggtgt
     5041 ctatcactat cgggtaagtc aggcgtttca tttaacggcg gatacgctga cggtgacgct
     5101 ctctgtcacc aatcaagggg cagagacgct gccatttggt accggctggc atccttattt
     5161 tccgttgtca ccacaaacgc ggattcaggc gcaggcgagc ggttactggc tggagcggga
     5221 gcagtggctg gcgggtgagt tttgcgagca gctaccgcag gaactggatt ttaaccagct
     5281 cgcgccgttg ccgcgccagt gggtgaacaa tggttttgcc ggatggaatg gtcaggcccg
     5341 catcgagcag ccgcaggaag gttatgccat catcatggaa acgacgccac ctgcaccgtg
     5401 ttatttcatc tttgtttccg accctgcgtt tgataaagga tatgcgtttg atttcttctg
     5461 tctggaaccg atgagccatg cgccggatga tcatcatcgc ccggaagggg gtgatctcat
     5521 tgcgctagca ccaggggaat caacaacttc agagatgtcg ttgcgggtag agtggctgta
     5581 atttattaaa tgccggataa ggcgttcacg ccgcatccgg catctttcct aacgccgatc
     5641 gagagaaatt gcccccggcc cggtaattgc caatagcaag aacgcgccag cgatactgac
     5701 attcttccag aaattaatca tatttggccc aaccgcatcg ccggtcatat cccagtaatg
     5761 gtgaccaatc accgccgtac ccagcgtgta gaaaataaac agcaccgcca gcggacgggt
     5821 gaaaaagcca agcacgatta atatcgcggc gggcacttcc ataactaccg caataatcgc
     5881 tgccagcatc ggcattggcg cgcccaacga ggccatatat tggaccgtac cgtcaaagcc
     5941 catcattttg ggaaaaccca aaataatgaa aattaagacc acggcgatac gggcaattaa
     6001 taacaaaacg gggcgggcag ctccaaaatc aaaataacgt agtgtgttca taaccaatcc
     6061 ctttgcaaga taatgtgatt taaacgtaat acacactctt gcacctcgcc acgccccagg
     6121 cgttgagata ttcgctaatt atcctggtta cagtgattta agcgcgatgt tctcatctat
     6181 cagcagtttc gcatcaaacg ttttgccgct ctggatccat ttgcgaattg ggcgaatctc
     6241 acgcccctga ttcagcgtga ccgcaccgat aagcacgccg ttttgcagat taaaccaaat
     6301 cgccttctga gtttccgggt tgccacgaca aagcc
//
LOCUS       DAJVOY010000203         6297 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVOY010000203 DAJVOY010000000
VERSION     DAJVOY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.203"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            275..616
                     /gene="raiA"
                     /locus_tag="OQL90_003657"
     CDS             275..616
                     /gene="raiA"
                     /locus_tag="OQL90_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1370283.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    719..844
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            720..767
                     /gene="pheL"
                     /locus_tag="OQL90_003658"
     CDS             720..767
                     /gene="pheL"
                     /locus_tag="OQL90_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCS1370284.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            867..2027
                     /gene="pheA"
                     /locus_tag="OQL90_003659"
     CDS             867..2027
                     /gene="pheA"
                     /locus_tag="OQL90_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1370285.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGLADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2070..3191)
                     /gene="tyrA"
                     /locus_tag="OQL90_003660"
     CDS             complement(2070..3191)
                     /gene="tyrA"
                     /locus_tag="OQL90_003660"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1370286.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3202..4272)
                     /gene="aroF"
                     /locus_tag="OQL90_003661"
     CDS             complement(3202..4272)
                     /gene="aroF"
                     /locus_tag="OQL90_003661"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1370287.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4480..4845
                     /locus_tag="OQL90_003662"
     CDS             4480..4845
                     /locus_tag="OQL90_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1370288.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4995..5513
                     /locus_tag="OQL90_003663"
     CDS             4995..5513
                     /locus_tag="OQL90_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1370289.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5503..>6297
                     /gene="dgcN"
                     /locus_tag="OQL90_003664"
     CDS             5503..>6297
                     /gene="dgcN"
                     /locus_tag="OQL90_003664"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCS1370290.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRS"
BASE COUNT         1707 a         1608 c         1415 g         1567 t
ORIGIN      
        1 agtcagttgc ttaactgact ggcattacag cccttgagct gccgtttttt tattctgtta
       61 gttgtgaaac tgaagcgatt tagtcactat cgatctcatc aaatatggct cgctttgaga
      121 tattcctcaa gtaaaaaaac acctcttcct gcgatttctc acaaaaaaga ttcgttgaca
      181 aaaagtgaca aaattatgag attcccatca cacattttga catcaggaac ggtatgctga
      241 attcaccaag acgggaagac aagaggtaaa atttatgaca atgaacatta ccagcaaaca
      301 aatggaaatt actccggcca tccgccaaca tgtcgcagac cgtctcgcca aactggaaaa
      361 atggcaaaca catctgatta atccacatat cattctgtcc aaagagccac aagggtttgt
      421 tgctgacgcc acaatcaata cacctaacgg cgttctggtt gccagtggta aacatgaaga
      481 tatgtacact gcaattaacg aattgatcaa caagctggaa cggcagctca ataaactgca
      541 gcacaaaggc gaagcacgtc gtgccgcaac atcggtgaaa gacgccaact tcgtcgaaga
      601 agttgaagaa gagtagtcct ttatattgag tgtatcgcca acgcgccttc gggcgcgttt
      661 tttgttgaca gcgtgaaaac agtacgggta ctgtactaaa gtcacttaag gaaacaaaca
      721 tgaaacacac accgtttttc ttcgcattct tttttacctt cccctgaatg ggaggcgttt
      781 cgtcgtgtga aacagaatgc gaagacgaac aataaggcct cccaaaccgg ggggcctttt
      841 ttattgataa caaaaaaggc aacactatga catcggaaaa tccattactg gcgctgcgag
      901 agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcgg cgcgaactgg
      961 ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgagat attgatcgtg
     1021 aacgcgattt actggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt
     1081 acattactcg cctgttccag ctcatcattg aagattccgt attaactcag caggctttgc
     1141 tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca
     1201 aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca
     1261 ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggcctggccg
     1321 actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaacgac gtttacgatc
     1381 tgctgcaaca taccagcttg tcgattgttg gcgagatgac gttaactatc gaccattgtt
     1441 tgttggtctc cggcacgact gatttatcca ccattaatac ggtctacagc catccgcagc
     1501 cattccagca atgcagcaaa ttccttaatc gttatcctca ctggaagatt gaatataccg
     1561 aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt
     1621 tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt attgaagcga
     1681 atcagcaaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt aacgtgtctg
     1741 accaggttcc ggcgaaaacg acgttgttaa tggcgaccgg gcaacaagcc ggtgcgctgg
     1801 ttgaagcgtt gctggtgctg cgcaaccaca atctgattat gacccgtctg gaatcacgcc
     1861 cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc aatcttgaat
     1921 cagcggaaat gcaaaaagca ttgaaagagt taggtgaaat tacccgttca atgaaggtat
     1981 tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg
     2041 gatgatgtga atcatccggc actggattat tactggcggt tgtcattcgc ctgacgcaat
     2101 aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg
     2161 cggaaactgt caataaacgc ctgcttatcg ccctgcttca gcaactcaat cgcctcgccg
     2221 aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtcg
     2281 gcataaagct gcggatcctg agcaaacagt cgcccgacca tcgccagctc aaggcggtaa
     2341 atcggcgaag agagcgccag aagttgctca agctgaacat tttcttctgc cagatgcagc
     2401 ccataagcaa aagtagcaaa gtggcgcaga gcctgaataa acgccatatt ctgatcgtgc
     2461 tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc cagaaaccat
     2521 tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgctttgc caggctaccg
     2581 ctgtccgggc cgaacatcgg gtgtaacccc agtaccgggc catcgtgcgc cgccagcatg
     2641 gcctgtaatg gtccattttt cactgatgcc agatcaacca gaatacaatc tttcggtaaa
     2701 ggcggtaatt tgccgataac ttgctcagta acgtggattg gcacactaac aatcaccatt
     2761 ccggcatcgg caacaatatc agccgctcga tcccagtcat gttgctccag aatccgcacc
     2821 tgataacccg agagtgtcag catcttctcg aacaggcgtc ccatctgacc gccaccgccg
     2881 acgataacca ccggacgcag tgacggacaa agtgttttaa atcctttgtc gttttcactg
     2941 gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtaccccc
     3001 agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc cggaacataa
     3061 ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc cagacgcttc
     3121 gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat
     3181 tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg
     3241 atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga
     3301 tacaccgtat ttcatttcac tgcgcggttg ctcagaagac tgattgccct cgtggatatt
     3361 actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga
     3421 ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac
     3481 catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc
     3541 agggctatag ttcggcgctt taccaccgcg caggatgaca tgcccgtccg gattcccctg
     3601 agtttgtagc aacgcaacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc
     3661 ggcggcgcgc atagcgttaa ttgctgttgc cagactgccg tcggtgccgt ttttaaaacc
     3721 aaccggcatg gaaagcccgg aggccatttc acggtgagtt tgcgattccg ttgtacgagc
     3781 accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc
     3841 ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg
     3901 cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatta accctttcca
     3961 gccgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct
     4021 gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt ccagatcatg
     4081 aatggaacaa ggaccacaaa ctaccagcag acgaggatcg cgcccggcga taatatctga
     4141 aatggtttta cgcgagtcag caatctgggc ttcttgttgc aggctcaatg gaaaagcggc
     4201 cttcagttgt tccggagtca ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc
     4261 gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat cctcagcgag
     4321 gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta cgcttttctt
     4381 tacacgaaca atttactcgc gctttgatgc ttatttcgta caaataaatt tacaccccct
     4441 ttaaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc
     4501 cccttattgg ctttactggt tagcggatgt cagattgatc cttatactca cgcgccaacc
     4561 ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg cagcgccata
     4621 aaagatgacg atgcatttgg cgattcacag gcggatcgcg gtctatacct taaaggatat
     4681 gccgaaggac aaaagaaaac ttgccagacc gattttactt atgcccgggg actttccggt
     4741 aaaagctttc ctgccagctg taacaatgtt gaaagtgcca gccaactgca tgaagtatgg
     4801 caaaaagggg ctgatgaaaa tgccagcaca atacgattaa attaattaaa accccacaaa
     4861 taaattaaac ataagatttt actcattgtg ttttatttct cacattgatg acgatcgcat
     4921 gaaaaaatga tattttaatg agcattctga aaaaatatcg atgcatttcg agcgaagaag
     4981 gtggggatcc ctgaatgcgt ttttctcacc gactgttctt acttctgatt ctcctgctga
     5041 cgggagcacc aatactcgct caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg
     5101 tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta
     5161 tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca tctttgcctt
     5221 atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc aatggttttt
     5281 attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaagg
     5341 cgttattatt aattgccaaa caaaataccg agtgcattat tggtagcgcc ttttgtctga
     5401 tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg cgtagcggtg
     5461 taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga taacgataat
     5521 tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata
     5581 tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct gaccctgaaa
     5641 cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca
     5701 gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct gggccagcaa
     5761 gggcaatttt caactgcaga agtacgtgat aagcagcaaa atattctggc gtcctggcat
     5821 tacacccgta aggatccagg cgatactttc agcaatttca taagccactg gctcttcccc
     5881 gcccccatca ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc
     5941 gctcgcgaca gttcaatcag ccattttatc tggttttcgc tcgccgtact gaccggttgt
     6001 attctgctgg catcaggcat cgcaattacc ctcacccgcc atttgcacaa tggcctagtg
     6061 gaagcgctga aaaatatcac cgatgtcgta catgatgtgc gttccaaccg caatttttcc
     6121 cgacgagttt ctgaagaacg tatcgctgag tttcaccgct tcgctctcga cttcaacagt
     6181 ctgctggatg aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc gcagcttctg
     6241 cgtaccgcgc tacatgaccc attaaccggg ctggctaacc gcgcagcgtt tcgtagc
//
LOCUS       DAJVOY010000204         6154 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVOY010000204 DAJVOY010000000
VERSION     DAJVOY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.205"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..492)
                     /gene="ssb1"
                     /locus_tag="OQL90_003665"
     CDS             complement(<1..492)
                     /gene="ssb1"
                     /locus_tag="OQL90_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1370291.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAP"
     gene            746..3568
                     /gene="uvrA"
                     /locus_tag="OQL90_003666"
     CDS             746..3568
                     /gene="uvrA"
                     /locus_tag="OQL90_003666"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1370292.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3603..3959)
                     /locus_tag="OQL90_003667"
     CDS             complement(3603..3959)
                     /locus_tag="OQL90_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1370293.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLRPEEKRKLLVQL"
     gene            complement(3963..4379)
                     /locus_tag="OQL90_003668"
     CDS             complement(3963..4379)
                     /locus_tag="OQL90_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1370294.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLANMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4490..5203)
                     /gene="aphA"
                     /locus_tag="OQL90_003669"
     CDS             complement(4490..5203)
                     /gene="aphA"
                     /locus_tag="OQL90_003669"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1370295.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5605..5827
                     /locus_tag="OQL90_003670"
                     /pseudo
     CDS             5605..5827
                     /locus_tag="OQL90_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1546 a         1528 c         1542 g         1538 t
ORIGIN      
        1 cggcgctgcc ggagcggact gctgcgggcg agactgcgcg ccgccgctga actgattgcc
       61 accctgcggc tgctgaggct gaccccaacc gccctgcggc tgaccaccac cgatattacc
      121 acctgccgga gcgccaccac cctgacgacc acccagcatc tgcatggtgc cgccaacgtt
      181 caccacgact tctgtggtgt agcgatcctg accggattga tcggtccatt tacgggtacg
      241 cagctgacct tcgatataaa cctgagaacc tttacgcaga tattcgctgg ccacttctgc
      301 cagtttgccg aacagcacaa cgcggtgcca ttcagtctgt tctttcatct cgccggtcgc
      361 tttatcacgc caggattcgg aagtagccag cgtaatgttg gcaactgcgc caccatttgg
      421 catgtagcgt acttccgggt cctgacccag attaccaacg agaataacct tgtttacgcc
      481 tctgctggcc atgttcgtgt ctcctgaaaa aaatcgttct gaataagtgt aaacgcgcga
      541 ttgtaccatt accaatagcg cttttactat gttgtgacct cggttccggg aaacaaacct
      601 ggccagacat tgttacacaa cactccggat aatgcattcc aatactgtat attcattcag
      661 gtcaatttgt gtcataatta accgtctgtg atcgccggta gcaccatgcc accgggcaaa
      721 aaagcgttta atccgggaaa ggtgaatgga taagatcgaa gttcggggcg cccgcaccca
      781 taatctcaaa aacatcaacc tcgttatccc ccgcgacaag ctcattgtcg tgaccgggct
      841 ttcgggttct ggcaaatcct cgctcgcttt cgacacctta tatgccgaag ggcagcgccg
      901 ttacgttgaa tccctttccg cctacgcgcg gcagtttctg tcactgatgg aaaagccgga
      961 cgtcgatcat attgaggggc tttctcctgc catctcaatt gagcagaaat cgacgtctca
     1021 taacccgcgt tctacggtgg ggacaatcac cgaaatccac gactatttgc gtttgttgtt
     1081 cgcccgcgtc ggcgaaccgc gctgtccgga ccacgacgtc ccgctggcgg cgcaaaccgt
     1141 cagccagatg gtggataacg tgctgtcgca gccggaaggc aagcgtctga tgctactcgc
     1201 gccaatcatt aaagaacgca aaggcgaaca caccaaaacg ctggagaacc tggcaagcca
     1261 gggttacatc cgtgctcgta ttgatggcga agtctgcgat ctttccgatc cgccgaaact
     1321 ggaactgcaa aagaaacaca ctattgaagt ggtggttgat cgcttcaagg tgcgtgacga
     1381 tcttacccaa cgtcttgcgg agtcgtttga aaccgcgctg gagctttccg gtggtacagc
     1441 ggtagtggcg gatatggacg acccgaaagc ggaagagctg ctgttctccg ccaacttcgc
     1501 ctgcccaatt tgcggctaca gtatgcgtga actggagcca cgactgtttt cgtttaacaa
     1561 cccggcaggt gcctgcccaa cctgtgacgg ccttggcgta cagcaatatt tcgatcctga
     1621 ccgcgtgatc caaaaccccg agctgtcact ggctggcggt gcgatccgtg gctgggatcg
     1681 ccgcaacttc tattacttcc agatgctgaa atcgctggca gatcactata agttcgacgt
     1741 cgaagcgccg tggggcagcc tgagcgcgaa cgtgcataaa gtggtgttgt acggttctgg
     1801 caaagaaaac attgaattca aatacatgaa cgatcgtggc gatacctcca tccgtcgtca
     1861 tccgttcgaa ggcgtgctgc acaatatgga gcgccgttat aaagagacag aatccagtgc
     1921 ggtacgtgaa gaattagcca agtttatcag caatcgccca tgcgccagct gcgaagggac
     1981 gcgtctgcgt cgggaagcgc gccacgttta tgtcgagaat acgccgctgc ctgctatctc
     2041 cgacatgagc atcggtcatg cgatggaatt cttcaacaat ctcaaactag ctggtcagcg
     2101 ggcgaagatt gcagaaaaaa tccttaaaga gatcggcgat cgtctgaaat tcctcgttaa
     2161 cgtcggcctg aattacctga cactttcccg ctcggcagaa acactttccg gcggtgaagc
     2221 ccagcgtatc cgtctggcga gccagattgg tgcgggcctg gttggcgtta tgtatgtact
     2281 ggacgagccg tctatcggcc tgcaccagcg cgataacgag cgcctgttgg gtacgcttat
     2341 ccatctgcgc gatctcggta ataccgtgat tgtggtggag catgacgaag acgcaattcg
     2401 cgccgctgac catgtgatcg atatcggtcc tggtgcgggt gttcacggcg gtgaagtggt
     2461 cgcagaaggt ccgctggaag cgatcatggc ggtgcctgaa tcgttgaccg ggcagtacat
     2521 gagcggtaaa cgcaagattg aagtgccgaa gaaacgcgtt ccggcgaatc cagaaaaagt
     2581 gctgaagctg acaggtgcac gcggcaacaa cctgaaggac gtgacgctca cgctgccagt
     2641 cggtctgttt acctgcatca caggggtttc aggttccggt aaatcgacgc tgattaacga
     2701 cacactgttc ccgattgccc aacgccagtt gaatggtgcg accatcgccg aaccggcacc
     2761 gtatcgcgat attcaggggc tggagcattt cgataaagtg atcgatatcg accaaagccc
     2821 aattggtcgt actccacgtt ctaacccggc gacctatacc ggcgtgttta cacctgtgcg
     2881 cgaactgttt gcgggcgtac cggaatcccg tgcgcgtggt tatacgccag gacgtttcag
     2941 ctttaacgtc cgtggcgggc gctgcgaagc ctgtcagggc gacggtgtga tcaaagtgga
     3001 gatgcacttc ctgccggata tctacgtgcc gtgcgatcag tgcaaaggta aacgctataa
     3061 ccgtgaaacg ctggaaatta agtacaaagg caaaaccatc cacgaagtgc tggatatgac
     3121 catcgaagag gcgcgtgagt tctttgatgc ggtgccagct ctggcgcgta agctgcaaac
     3181 gttgatggac gttggtctga cgtacattcg cctggggcag tccgcaacca cactttctgg
     3241 tggtgaagcc cagcgcgtga agctggcgcg tgagctgtca aaacgcggca ccgggcagac
     3301 gctgtatatt ctcgacgagc cgaccaccgg tctgcacttc gccgatattc agcaactcct
     3361 cgacgtgctg cataaactgc gcgatcaggg caataccatt gtggtgattg agcacaatct
     3421 cgacgtgatc aaaaccgctg actggattgt cgacctggga ccagaaggcg gcagtggcgg
     3481 cggcgaaatc ctcgtctccg gtacgccaga aaccgtcgcg gagtgcgaag cttcgcatac
     3541 ggcacgcttc ctcaagccga tgctgtaatc gttaaggccg ctttctgagc ggccttttcc
     3601 tttcagagtt gtaccagcaa tttacgcttt tcttccggtc gtaaattcac tgcctgctga
     3661 taagacgcat ccaccagata atagatttgc gaatccggca gcgaaccgtc gagatacacg
     3721 gtgctccagt gcgctttatt cagatggcgg ctaggacgca catcgctgtg ctgctgacgt
     3781 agcagctccg ccagctccgg gctggttttc agcgaaacag ctgggcgatt ttctacttct
     3841 ttcaccatcg caaacagtac atcttccact ttgatctgcg tcgctttcca gtcgttatgc
     3901 acgctctgtt ctgcgcctag ttttgccatg caatattgta gcaactccga aatggtcatt
     3961 ttttactccc cttgtagtgt cgcgatgatg cgacgcgatc cgccgtggat gcgatgttcc
     4021 cccagccaga tgccttgcca ggtgccggtc tgaatacgcc ctttatgtaa cggcaataca
     4081 agcgatgttc cctgcattga ggatttgata tgagaaggca tatcgtctgc tccctcatag
     4141 tcatgctcat aatttccgtt gtcgggaacg gtgcggagga aaaaacgctc catgtcgtgg
     4201 cgtacggtgg gatcgcagtt ctcattaagt gtcagagagg cggaggtatg ttgcagcaac
     4261 agatgcagta agccgatgtt aacgcgcggc atattagcca gctgattcag aatttcatcc
     4321 gttaccagat gaaacccacg agatttggcg ctaagcgtga gcgtcttttg ataccacatg
     4381 tgctgctcct tgataaaact ctcttaatca gtttgcagca agacagcgaa aggataaagg
     4441 tgtgattaaa aaaacagcat tcgggagagc atcacgctct cccgctctgt cagtattctg
     4501 aattgacgat cacctcttca ccaaacgcac ccgcttgtgg caagggtttg taggtagagt
     4561 tggaggcgcg cagaatgcgg ataccacgag cgccgacatc gcgtgcggcg gtaatatcat
     4621 tatcagaatc gccataaaaa attcggatat ttttatcctg cagccattgc gattttgtat
     4681 tttgccctgg tttatcgccc gcaaagatca ccggattcat gttggtggca ggaatatgaa
     4741 aattatccgc cagcgttttt gaaaccgttt ctgttttcgt cggactacga ccagtcacaa
     4801 agaagatcgc gtcaccgcgg cgtacatgca tatcaatcag ctggcgagcg acctcttttg
     4861 gaatgctgaa ttcatcccag ccattgttca ttttttccca gaacacagga tttttcagat
     4921 aatcttcgct ttctggcgag aaggtttttt tgccgcgcca gaagcccggg ctggaaaaaa
     4981 gtaccgtgtc atcgatatca aaccccaccg ccattggcgg acgccctgcg aggctatttt
     5041 caatttgtgc gaccgaaacc caatgaattg gtgcctgttc agcaagcctg gcaacgttag
     5101 taccagggtt aagcggtgaa ggagatgagg ccagggcaac agcggaactg tttagagcga
     5161 acaataagca aacggcactg attgcctgtg tgatcttgcg catatttttc cctaaatagt
     5221 cagtatgttg aaacttttta ttgtgagatt tgttgcaaaa accatctgac cataacgcca
     5281 gctgcagggg aaaggaagga ttatctgcgg tttttgtgat gaaaagcagg atgattatat
     5341 ttaacccagt aaatacgttc ctgtcttttt ttgtataaaa aatgtttatt actcgatgta
     5401 aggatacatt aattttacgt ttattatgat gaatcctctg caaatgagcg aaatttgata
     5461 gaacatttat ctgctgtacg atttaatgaa cttaatgata cagcattaca aaaacaatcg
     5521 aagtttataa agatgattcc tgattgacca gaaccctatc ctacaatggt gattaatttt
     5581 attactcgcg atggagatga tgatatgaat atatcatata tgaatagcaa caaaacgaca
     5641 tctttacctg ttgagctaga tgcactaaat aataaagata tctcttatgc caaagatttt
     5701 tcttatgtca aagatttctt tttatacata gaaacactgt taaaaattgc aaaagatttt
     5761 tgtagacttg gagaagaagt atcaagttct attgcaagta aagtttttca cgcatttatt
     5821 gatttagtta acaaaatcag gggtaagaaa gactttatgt atatttgcac gctttgctgt
     5881 tttgccgagg aggttaaagg tgattattct cattacagga cctttttatt tgatattggt
     5941 aatcaatata aggttaaact tacacaaagc ggaaaaaaag agttctcttt aacattagaa
     6001 tttaacgata ctataattga atctcagaaa gtcacaggca ataaggcaaa gcatattctt
     6061 gaggatatag aaatattcta tcgtaacaaa cccgatactt attattaaat ttaataatgg
     6121 ttagatgtgc agaaattatt actccacacg aaaa
//
LOCUS       DAJVOY010000205         6102 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVOY010000205 DAJVOY010000000
VERSION     DAJVOY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.207"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(24..605)
                     /locus_tag="OQL90_003671"
     CDS             complement(24..605)
                     /locus_tag="OQL90_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370296.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OQL90_003672"
     CDS             complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OQL90_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCS1370297.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4870..5874)
                     /locus_tag="OQL90_003673"
                     /pseudo
     CDS             complement(4870..5874)
                     /locus_tag="OQL90_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5871..6102
                     /locus_tag="OQL90_003674"
                     /pseudo
     CDS             5871..6102
                     /locus_tag="OQL90_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1520 a         1360 c         1368 g         1854 t
ORIGIN      
        1 gggctaaaat accgggacag agatcaggcg tgattaatct gcagcatttt taataacaga
       61 agtactgagc ttactttacc tttgatgatg taatagttat ttccgtcggt actcattttt
      121 ccctgaaata atgtcacgga tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc
      181 atatttttaa tgagctgaga tccgggtggc aaggggtatt tgctgtcaga tactgggcga
      241 ctgagtctga tgtggcgcgt atacagcccg ttttcgtaca gggtggcctc tgttatggtg
      301 tcagtgacca gcatgtggga gacaatgctg tattcttttt taaagcaccc accagaaacg
      361 gtgattgtaa tgataatggt cttctcgatc gagatgtaaa tacccgtttc gttatactgt
      421 ccgtatcgga accatgagaa ctcagttgca ttccagccat gctccagaat atgtgcattt
      481 ttttgcctga tagtttcttt ttttattcgc tcatgtttaa ggcataacag gcagataaat
      541 aatgtcagaa aaagaaagct aataatgaga gcaagagcga tatagtctgt cacagggtaa
      601 tacatattct ctccggtaga agaagggccg caaacgcggc ccgggctgtt atcagaaaga
      661 gtaacggaag ttggcgttga tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc
      721 aaactcaaga ccaaagcgaa gattatcgcg aatttcggcg ttgagaccaa cattcatgag
      781 aatacgaccg tctttttcac ctttgatacg tttctcaccg gacgcatcac gcagtacggt
      841 ttcaccgttg gcaaacaggt caaactggta gccaaggccg gcgcgggctg tgactttcca
      901 gtccttaccg gagaaggatt tacccacatc aacaccggta cgcccaatca gcggattaaa
      961 gtccttatcc ttcatggtca cgctcattcc gcgatcctgc cagtcgaacc gtttacctga
     1021 tacggcccca taaaccagtt ctgcctgcgg ctcaatccag gagtcttctg ttacatgata
     1081 gcggtagcct gcttcagcac ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag
     1141 cccagcaaag gtcgccgagt actcattatc atggtgaaca tacttactaa tcaggtcgat
     1201 ataggcacca gagtcaaata tagcggaagc atacagacct gccccgacgg acttcgtctt
     1261 gccactgaat gcattactgc tggcatgact gtcggtatac gtcatagtca gaccagtgaa
     1321 aaggtcaagt ccatccagct catgtttttt atccacacca atctgcacat gggtgtagtt
     1381 gtcactgtat ccaccagaag ctgaacctgc tccgctcatg atgcgggccc atgcaccgac
     1441 ctcgccattg gtgtcacgca gatcccccat tcgtttattc aggttgttga cttctgtcag
     1501 gaaggctttg taattaccgg acatatacga ttttgctgca ctggaagatg ctgtattttc
     1561 agccacctta tatccggtca gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat
     1621 cacaggagat atgttgctga aacctatggc ctgagtactg gctttaaata tctctttatt
     1681 gcttcctttt ggggctgtta tcaggtcaac gtttaatcct gagttattat ttcccacttt
     1741 ctgtatatcg accgacagac tgttattgct tccggttaac tgatttttaa caatgagttt
     1801 atccgattga tttacagact ggctcagatc agcccgcata atcacagcag tattagtggc
     1861 atccagtgtg tttactgtca cagtatggaa acccgaattc tctatgttac taaacaacaa
     1921 aagactgtcg cgggttttta atgattgcag ggctgactgt ccgtttactc tccataaggt
     1981 gttatacatg ctggctgagg cagcaggagc atcaatacct ccggtataag aagtatcaaa
     2041 gccacccagc aaacctgtag ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt
     2101 atactggtca acaccaaaca gaatagatgc ttttttgtct gattttatat taccattgac
     2161 ccaggcataa ttttgagcct ttaacgtggc accatcttct gtcatattat atccatctga
     2221 catatagatt gccgggctgt atttattatt ttctgtaggg gtgcctgtta atgtcagggt
     2281 tccggccagt tcaatagctt tatctacata catttttccc tgggcggtaa cagtagctcc
     2341 tttgttgata atcaggtctg tattaattaa ggataatgca ccagtcatag taacagttga
     2401 tttatcacca acagttattt tgctgttttg agcactcaga ctgccgttga aactactttc
     2461 gcctgctgaa tccccggagc gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt
     2521 tcttcctgag aacatgtcaa tgtatgcagt tttatttccc agaacaattt gagagttttt
     2581 agcctctatg tgtcctttta cattagcatc ccggcctata ctcagggttg cgttttctaa
     2641 atttaatttc cggaaattaa atttacgact ttcccagtcg ggctgggaaa aagaggctat
     2701 ctgattgttg gacttatatt ccgtattgta acgtttattt acagtgcttt cctggtcctt
     2761 tatggcagca gaatagtctt tttgacataa aaaaggaatt ggacagttgt tattgccttc
     2821 tttaaatatg gcatgttctg tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga
     2881 gatatcgttt ttgatatcga ctgagccatc aaagatcaga ttggcattat tgttatgttg
     2941 ctgggtagta ttgataatat gatttatgct tatattacct gatacattcc catggtacat
     3001 atatgcatca gtattagtaa gtgataatgt tgattgcttc acgtttgagt tcgttacggt
     3061 tgttcccgca tctgttgctg cgattttctg aaatgactgg tcatagccgt ttagatccag
     3121 tgttccgcca tttctggtaa aatagatttc gccattaccg ctttcgctta aagcgtcttt
     3181 ggcatttatt tttaccgtac ctttacctcc ggccatgtaa attttattga aggctttttc
     3241 agcactaagg atgacagtcc cattacctat tttaaggtta tttccctgtg ctatttttac
     3301 atccagagta ccactaccta ttttatgcag attatctccg gcaacgccct taacattcca
     3361 ttcaacggtg gtattattat caatatctat cccggcacct ttataagact tatcttttcc
     3421 gtacacttta tatgtctggt tcttatcgaa tataagcccg ccaatgccaa gatcggtatc
     3481 ctgcttgagc gttaattccc ctcctcctga gacagacagg tccttatctt ttataatcga
     3541 tgataactct tgttttttac catccagctg catagtgttt gtgaaattct tttttaaatt
     3601 gtttaccaga atattattaa ataacgagta gtttgtccag gctatattgg tactactgat
     3661 aaaaccaact gtactggtaa caccatggaa aacccatttt tgttgttctt tatcatatag
     3721 ataagaacca gaaccactat cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt
     3781 ataaatgtaa ctcttgtttt tccagtccag attaaaaaga cttgcgctta acaagtcggg
     3841 attatagttt tgtccaaaca gatattgttt gccgtttaat atcgttgagg ttgttccggc
     3901 acctgctctg aagccaatta tttgttcctt accgttgtag tttacgccat atcttttcat
     3961 cgcatcttcg ggagaaagtg agaagtcggc actctccgta acccctgttg tttccacgac
     4021 gaatttattc agacgatgaa ccgaaaagtc gccagatgat actctgttac ttgctttata
     4081 tgctgtctga tcccatgact gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc
     4141 agtaacactg tacgcacctc caattgatgt tgttgcacct ttgttggaaa cagcagaaaa
     4201 atcaggtaca ggtagttctg gaaaatgaaa tatctttcca tctttcatca ttaattgaac
     4261 atttgttgca cccggtctga actctcctct gttttgagca aggtcaagat aatcgcgggt
     4321 ccatatatta tttatatcca gcattgctgc aaatgctgtt tcaccataac ttccatatat
     4381 tgaggataaa ataataccga ggataacttt ctttcttgat gtaccgacac taactttccg
     4441 ggtcagttca gaaacagcaa ctaatccacc tgtaatatga ctatatttca gtgaataaat
     4501 tttattcata cgtgaactcc gatatttctg agttgtggtt atgggatagc atttaattac
     4561 aacggggaac tatcagtcat ggtacgtaac tgataagggt ataatgataa tcactgtcag
     4621 ttatatttgt atgacagatg taacaaaatg aaaaaaaaga taagcacaag tgattgattg
     4681 gtcagatatt gatcacctgg tgtttgtatt ttgacctttg cgatcaaaat ttgatctttg
     4741 ttgtttccgg tcaatccttt acaggttttc tgtattatcc gggggagtta agtctgtcga
     4801 tgtccgacag acttttgatg atggaaacaa catgcagcga taatgtgcat gctgttttgc
     4861 aggtggaact cagaaggtga actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc
     4921 gatcaggcct gcaacatcaa ggtgaaccac atcaaacggt gagatcccca ccccggccgt
     4981 gaatgcactg ccgtcgttac cggccagatt ctgacggtaa ccggcgcgca gctgtgccca
     5041 tttccaggca ttaaattctg cgccaatggc ggcaaactga cggttgctgt cggaggtgaa
     5101 accgctggcc ggggtcagat ccacatcaaa tgcggtggtg aacatcgcgt tgtgccagga
     5161 gacaccggcc gtcacctgcg ggcggatcct gaaggtttcc gtgataccgt tcacttcttt
     5221 tgtctcaata ctacggggga tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc
     5281 caggttcgta ctggcaccga tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc
     5341 gcctttgaag gcgctgctgt cataatttct gaccagcacg ttgtagttga acaggtcgac
     5401 gcgctggaat ttggggggga agcccagaga ccatttctgc ccggccgttt ccagttccgt
     5461 agccagagca atacccacat cagtgatcag tgccgcgcgg gcaaaggcct gtgatgtcag
     5521 gcggcttttg tccacctcct gcccggtgct gtttgccacc tcttccaggt aagtgaggtc
     5581 agcatcagag actttaccgt cgacactgac ggtgccgtgt gatttaacca tcagtgcggc
     5641 agagaggcga tcaccgggaa gggccgccac agcagaaacg cccagctgtg cggcggcatg
     5701 tgtgttacgg aagtcccgga ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg
     5761 gttactttcg atggcgcggt cgaaggcctt ccactcatca ctgatccggt cggcgttgtc
     5821 ggtgatatta tccggattgg agagctgcgc gccgacggac ggaagcagga tgctgggctc
     5881 atgcgcgacg caaaatccac gatgtatata tcagtactaa aagcgcgacg gcggaagaag
     5941 ctctgaaact aatcggtgaa ctgtacgcca tagagcacga tatacgcggg ttaccggtgt
     6001 ctgaacgcct ggcggtcagg caaatgcaga gtagaccgct cctgacttcc ctgtataagc
     6061 tgatgcagga gaaagaacac acgttatcga agaaatgtcg tc
//
LOCUS       DAJVOY010000206         6042 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVOY010000206 DAJVOY010000000
VERSION     DAJVOY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.208"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..226)
                     /gene="yneK"
                     /locus_tag="OQL90_003675"
     CDS             complement(<1..226)
                     /gene="yneK"
                     /locus_tag="OQL90_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS1370298.1"
                     /translation="MVTPISISNYIFLPDDFPVRNIAPQVKEVLKNFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFR"
     gene            complement(304..1185)
                     /locus_tag="OQL90_003676"
     CDS             complement(304..1185)
                     /locus_tag="OQL90_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1370299.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            1286..2674
                     /gene="sad"
                     /locus_tag="OQL90_003677"
     CDS             1286..2674
                     /gene="sad"
                     /locus_tag="OQL90_003677"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCS1370300.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2738..3664
                     /gene="glsB"
                     /locus_tag="OQL90_003678"
     CDS             2738..3664
                     /gene="glsB"
                     /locus_tag="OQL90_003678"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1370301.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3664..4023
                     /locus_tag="OQL90_003679"
     CDS             3664..4023
                     /locus_tag="OQL90_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCS1370302.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            4162..5580
                     /locus_tag="OQL90_003680"
     CDS             4162..5580
                     /locus_tag="OQL90_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1370303.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            5807..>6042
                     /locus_tag="OQL90_003681"
     CDS             5807..>6042
                     /locus_tag="OQL90_003681"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1370304.1"
                     /translation="MKTLNRRDIPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNE"
BASE COUNT         1439 a         1393 c         1616 g         1594 t
ORIGIN      
        1 cccgaaaaga agtcctctga atatatgata agttatcaag gttgttaaat atcttttttg
       61 tggctgagtt ggtgtttaaa gacgtgcgcc actcttcatc acatattatg gtactgagtg
      121 catcaataaa attctttaaa acttctttta cctgaggtgc aatattacgt acgggaaaat
      181 catcaggaag aaatatgtaa ttactgatgc ttattggcgt aaccatattt gccccatata
      241 gtcataagtc atcggaacat cactatatag ggcatttgag ctgcaagaac tatcaaaaat
      301 agctcattgg taaccctgtt ttgccgagtc aggcacatcc agtagttgaa taaatgcctc
      361 aagcggacgc gttttcgcac cacgacgcca gaccagccag gttgttaacc aacgccattg
      421 ctcagctaac ggccacgctt caacctggtg atgccccggc atactttcca gcatagagcg
      481 tggaataagc gcaatgcctg ctcctgcgac cacacaggcc aacatcccgt gatacgactc
      541 catctcgtga atagttcccg gagcggcacc gtcagcatga aaccagctct cgaagtggcg
      601 acgatacgaa caattggcgc ggaaggcata aatgttactg ccgttaacct gactggcgcg
      661 gattactggc gcatgacctt gtggcgtgac gatcatcagc tcctcgcggt ataccggtat
      721 cccgtcaatg gcagtatggt taatgggccc atcaataaat gccgcattca gttttccctc
      781 cagcactcct tccagcatag tgccggaagg gccggtggaa agtgaaaact gaattttggg
      841 ataacgatgg ttgaattccg ccagcgtggc cggaatgcgc actgctgcgg tgctttccag
      901 cgaaccaaga gaaaataaac cttgcggctc atcgccagcg acaacgctcc gcgcttcatc
      961 cactaacgcg agaatttgtt ggctatagcg taaaaagtta tgcccggcag gagagagacg
     1021 taaacgctga ttctcgcgaa taaacagatc tacccccagt tctgtttcca gctggcgtag
     1081 acggatagtg agattcgacg gcacgcgaag cactattgct gctgcctggg ttatgctgcc
     1141 agcttcggca accgcgttga acatctccag ttgggtcaga tccataatat tctcgattcg
     1201 tgaataagtg gcttaatatt attcatttta aagcaagagt aaatctgcgt atcttcatac
     1261 catgactcat aaaggagata ccccgatgac cattactccg gcaactcatg caatttcgat
     1321 aaatcctgcc acgggtgaac aactttctgt gctgccgtgg gctggcgcta acgatatcga
     1381 aaacgcactt cagctggcgg cagcaggctt tcgcgactgg cgcgagacaa atatagatta
     1441 tcgtgctgaa aaactgcgtg gtatcggtaa ggctctgcgc gcccgtagcg aagaaatggc
     1501 gcaaatgatc acccgtgaaa tgggcaaacc aatcaatcag gcgcgcgctg aagtggcgaa
     1561 atcggcgaat ttgtgtgact ggtatgcaga acatggtccg gcaatgctga aggcggaacc
     1621 tacgctggtg gaaaatcagc aggcagttat tgagtatcga ccgttgggga cgattctggc
     1681 gattatgccg tggaactttc cgttatggca ggtgatgcgt ggcgcggttc ccatcattct
     1741 tgcaggtaac ggctacttac ttaaacatgc gccgaatgtg atgggctgtg ctcagctcat
     1801 tgcccaggtg tttaaagatg cgggaatccc gcaaggcgta tatggctggc tgaatgccga
     1861 caacgacggt gtcagtcaaa tgattaaaga ttcgcgcatt gctgctgtca cggtgaccgg
     1921 aagtgttcgt gcgggagcgg ctattggcgc acaggctgga gcggcactga aaaaatgcgt
     1981 actggaactg ggcggttcgg atccatttat tgtgcttaac gatgccgatc tggaactggc
     2041 ggttaaagcg gcggtagccg gacgttatca gaataccgga caggtttgtg cagcggcaaa
     2101 acgctttatt atcgaagagg gaattgcttc tgcatttacc gaacgttttg tggcagctgc
     2161 ggcagccttg aaaatgggcg atccccgtga tgaagagaac gctctcggac caatggctcg
     2221 ttttgattta cgtgatgagc tgcatcatca ggtggagaaa accctggcgc agggtgcgcg
     2281 tttgttactg ggcggggaaa agatggctgg ggcaggtaac tactatccgc caacggttct
     2341 ggcgaatgtt accccagaaa tgaccgcgtt tcgggaagaa atgtttggcc ctgttgcggc
     2401 aatcaccgtt gcgaaagatg cagaacatgc gctggaactg gctaatgata gtgagttcgg
     2461 cctttcagcg accattttta ccaccgacga aacacaggcc agacagatgg cggcacgtct
     2521 ggaatgcggt ggggtgttta tcaatggtta ttgtgccagc gacgcgcgag tggcctttgg
     2581 tggcgtgaaa aagagtggct ttggtcgtga gctttcccat ttcggcttac acgaattctg
     2641 taatatccag acggtgtgga aagaccggat ctgacgcgac atattcagct ctgatatact
     2701 cgcaggtctt ttcagacctg cggtccagga gtagaaagtg gcagtcgcca tggataatgc
     2761 aattttagaa aacatcttgc ggcaagtgcg gccgctcatt ggtcagggta aagtcgcgga
     2821 ttatattccg gcgctggcta cagtagacgg ttcccgattg ggcattgcta tctgtaccgt
     2881 tgacggacag ctttttcagg ccggagacgc gcaagaacgt ttttccattc agtctatttc
     2941 caaagtgctg agtctcgttg tcgccatgcg tcattactcc gaagaggaaa tctggcaacg
     3001 cgtcggcaaa gatccgtctg gatcaccgtt caattcttta gtgcaactgg aaatggagca
     3061 gggtattccg cgtaatccgt ttattaatgc tggtgcgctg gtggtctgcg atatgttgca
     3121 agggcgatta agcgcaccac ggcaacgtat gctggaagtc gtgcgcggct taagcggtgt
     3181 gtctgatatt tcatacgata cggtggtagc gcgttccgaa tttgaacatt ccgcgcgaaa
     3241 tgcggctatc gcctggctga tgaagtcgtt tggcaatttc catcatgacg tgacaaccgt
     3301 tctgcaaaac tactttcatt actgcgctct gaaaatgagc tgtgtagagc tggcccggac
     3361 gtttgtcttt ctggctaatc aggggaaagc tattcatatt gatgaacctg tggtgacgcc
     3421 aatgcaggcg cggcaaatta acgcgctgat ggcgaccagt ggtatgtacc agaacgcggg
     3481 ggagtttgcc tggcgggtgg ggctaccggc gaaatctggc gttggtggcg gtattgtggc
     3541 gattgttccg catgaaatgg ccatcgctgt ctggagtccg gaactggatg atgcaggtaa
     3601 ctcgcttgcg ggtatcgccg tccttgaaca attgacgaaa cagttagggc gttcggttta
     3661 ttaatgcagt ctctcgatcc actcttcgcg cgtttatccc gttcaaaatt tcgctctcgc
     3721 tttcgtctgg gcatgaaaga gcgtcagtat tgcctggaga aaggcgcgcc agtcatcgaa
     3781 caacatgcgg cggattttgt cgctaaacgt cttgctccgg cattaccggc taatgacggt
     3841 aagcaaacac ccatgcgcgg gcatccggtg tttatcgctc agcacgccac cgcgacctgc
     3901 tgtcgcggtt gtctggctaa gtggcataac attccgcagg gtgtttcgtt aagcgaagaa
     3961 caacaacgtt atatcgtggc ggtaatctat cactggctgg ttgtacagat gaatcagcca
     4021 tgagaattgc ggaaaacgat aaaatagcat tcccagggta cctttatttg ccgccgctgt
     4081 tagcgaacat gctatccttt atgagaattt acttattacg ttgagataat tgccacccgc
     4141 aattgtatgg acagataatt aatgcatgta caccccattt caactttccg tttgttccag
     4201 gaaggtcatc tgctacgtaa tagcatcgct atttttgcgc taaccacgct gttctatttt
     4261 attgttgcag agttacggct ggttcacgaa ctttctcttt tctggccgct gaatggcgta
     4321 atggcggggg tgtttgcccg ctatgtctgg cttaatcgac tgcattacta tgcgatcagt
     4381 tatgtggcga tgctggttta tgatgccata accaccgaat ggggactggt ttcactggtt
     4441 atcaatttct ccaatatgat gtttattgtt accgtcgcct tactggtcgt gcgggataag
     4501 cgtctgggga aaaataagta tgagccagta agtgccttac ggctatttaa ttactgtctg
     4561 attgccgcat tattatgcgc tattgtcggg gcgattggtt cggtcagtat tgatagtctg
     4621 gatttctggc ctttgcttgc ggactggttc agtgagcaat tctcaacggg cgtgttgatc
     4681 gtgccttgta tgctgacgtt ggctattcct ggagtgctgc cgcgctttaa agcagaacag
     4741 atgatgcccg ccatcgcgct tattgtgtcg gttattgcct cggtagtcat tggcggagcg
     4801 gggagtctgg cgttcccgct gcctgcatta atctggtgtg cagtgcgcta tacgccgcag
     4861 gtaacatgtc tgttgacctt tgtcaccggt gcggtggaaa tcgtactggt ggcaaattcg
     4921 gtgattgata tctcggtcgg ttcgccgttc tccattccgg aaatgttctc cgcacgtctc
     4981 ggtattgcca cgatggcgat atgcccaatt atggtttctt ttagcgtggc agcgatcaat
     5041 tcgctaatga agcaagttgc gctgcgagcc gactttgatt ttctgactca ggtttactca
     5101 cggtccggtc tttatgaggc gctgaaaagt ccatcgctga aacagacaca acatctgacg
     5161 gtcatgctgc ttgatatcga ctatttcaaa agcattaacg ataactatgg acatgaatgt
     5221 ggcgataaag tgttaagcgt gtttgcccgg catattcaga agattgtcgg tgataagggg
     5281 ctggtggcgc gcatgggcgg cgaagaattt gctgttgcag tgccgtcggt gaatcctgtc
     5341 gatggtctgc taatggcgga aaaaatccgt aaaggcgttg aactgcaacc attcacctgg
     5401 caacaaaaaa cgctttatct cacggtaagt attggcgtcg gtagtggtcg cgcatcgtac
     5461 cgaacgctga ccgatgattt taataaattg atggtcgaag ccgatacgtg tctgtatcgc
     5521 tcgaagaaag atgggcgcaa ccgtaccagc actatgcgtt acggtgagga agttgtctga
     5581 catctttatt cattccgcgc gatttgaatg caatgtgttc taccacttta tgatccagga
     5641 aaaggcgtat ttcctcgatt aaaccatgat ccgcgccaca cttttctcat aaaactgctc
     5701 attaatcatc aggagttgat caaaacaggg gttcggcgta ttaaattttg ctaacctgtc
     5761 gtaccaattc agacgggttg tatgactaat cagaagggaa cccattgtga aaacactaaa
     5821 tcgtcgcgat attcccggtg cacagtatcc agaacgtatc attcagtttg gtgaaggtaa
     5881 cttcctgcgc gcctttgttg actggcaaat cgatctcctg aatgagcaca ccgatctgaa
     5941 ttctggcgtg gtcgttgttc gtccgattga aacttcattc ccgccgtcac tgagcacgca
     6001 ggatggtctg tacaccacca ttatccgtgg cctgaatgag aa
//
LOCUS       DAJVOY010000207         5982 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVOY010000207 DAJVOY010000000
VERSION     DAJVOY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.209"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..157
                     /locus_tag="OQL90_003682"
     CDS             <1..157
                     /locus_tag="OQL90_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370305.1"
                     /translation="ILQLCRNDSLMIGRESFSVDRITPDDGGCSYIRLRREGAGNVRA
                     GGYYEGA"
     gene            144..731
                     /locus_tag="OQL90_003683"
     CDS             144..731
                     /locus_tag="OQL90_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370306.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            728..1129
                     /locus_tag="OQL90_003684"
     CDS             728..1129
                     /locus_tag="OQL90_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370307.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1140..1880
                     /locus_tag="OQL90_003685"
     CDS             1140..1880
                     /locus_tag="OQL90_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1370308.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1939..2325
                     /locus_tag="OQL90_003686"
                     /pseudo
     CDS             1939..2325
                     /locus_tag="OQL90_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
     gene            2334..2662
                     /locus_tag="OQL90_003687"
                     /pseudo
     CDS             2334..2662
                     /locus_tag="OQL90_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2634..5675
                     /locus_tag="OQL90_003688"
     CDS             2634..5675
                     /locus_tag="OQL90_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1370309.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5675..>5982
                     /locus_tag="OQL90_003689"
     CDS             5675..>5982
                     /locus_tag="OQL90_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370310.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     "
BASE COUNT         1450 a         1369 c         1941 g         1222 t
ORIGIN      
        1 tattttacag ctgtgtcgca atgattcgct gatgattggc cgggagtctt tcagcgtgga
       61 tcgtattact cctgatgacg gcggatgtag ttatatccgg ttgcggcgtg aaggagcagg
      121 aaacgtaagg gcagggggat attatgaggg ggcttgagaa tgccatccgc aatctgaaca
      181 gtcttgatac ccgtatggtg ccgcaggcca gcgcatgggc agtaaaccgg gttgctgcaa
      241 aagttgtctc ggttgccacc cggcaggtcg ccaggaatac cgttgcggga gataaccagg
      301 tgaagggggg ccccctgaaa ctggtgcgtc agcgcgtccg ggtatttaaa gccagtccgg
      361 caggaaaaat gtatgcccgt atccgtgtta accggggcaa tctgcccgcc attaagcttg
      421 gcacaacatg ccggcgtgcc ggtgacggac tgaaggtggg gaaatacttt tttcgtggtg
      481 cgtttatcca gcagctggcg aacggtcgct ggcatgttct caggcgtctt cctgaagcgt
      541 gttttgaaac aggaaaggac agtaaggggc ggcccagaaa aaatcgtctg cccgtggagg
      601 ttgtgaaaat tccgctggcc ggaccgctga cacaggcatt tgaggatgcc cgcgaccgca
      661 tcattgccgc ggaaatgccg aaacagctgg ggtatgccct gaaacagcaa ctgaggatac
      721 atttgggatg aacaggcaca cacaaatccg ccaggcagta ctggcaagcc tgaagggcga
      781 atgcgggggg aatgtcgtgc tttttgacgg gcttccggca tttattgatg cgcaggaact
      841 gcccgccgtg gcggtgtggc tgagtgatgc gcagtacacc ggaaaaatga cggatgagga
      901 cgactggcag gctgttctgc atattgccgt tttcgtccgt gcacaggcgc cggattctga
      961 gctggatacg tggatggaga acaccatatt tccggccatg aaggatattc cggtgctttc
     1021 cggactcatc gacaccatga tcccactcgg ttttaactat caacgtgata atgagatggc
     1081 cacctgggcg atggcggaaa tcacgtacca gatcacgtac acgaattaag gaggtggcaa
     1141 tgaccacacc aaatccactg gcaaagacga aaggtgcggg aacgacgttc tggatgtaca
     1201 ccggcaaggg cgatgcgttt gcgaaccctt tgtcggacac tgactggctg cgtcttgcga
     1261 tggtgaagga tctgcaacct ggcgaaatga ccgctgatgc agaagatgac acttatctcg
     1321 atgatgaaga tgcagactgg aaaacgacaa cccaggggca gaaatccgtc ggtgatactt
     1381 tggcgacgct ggcctggcgt ccgggtgaca gcgggcagaa aaaactggtt cagttgttcg
     1441 attccggtga agtctgcgcg tttcgtatca aataccccaa cggtacggtt gatgttttcc
     1501 gtggctggct gagttcactg ggtaaaacca ttacctcaaa agaggtgatg acccgtactg
     1561 taaaaatcag cggtgtgggg cgtccgtatc tggcagagga gggcgctgaa accgtgggtg
     1621 ttaccggact gacggtggca ccggcatctg tcagtgtcaa agcgggagca accaccacac
     1681 tgacctttac agtaaaacct gacggggcca gtgacaaagc gatcagtgtg cattcgtcag
     1741 atcctcagac tgcttcggtg accctgagcg ggcttgtggc cacggtgaaa ggcgtgaagc
     1801 agggcagtgt cagcatcgtg ggcatgacct ctgacggaga gtttgtggca gtggctgcgg
     1861 tgaccgtcag cgcaccataa caggacgata ctcatcattg ccccggttat ccggggcttt
     1921 ttgcatccgg agaacatgat gtttctgaaa caggatacgt ttaattatga aaaacagtcc
     1981 gtggtgctca gtgagctgtc cgggctgcag agaattgaat atctgacgtt tgttcagcag
     2041 cgaacggcaa agtttgatgc acaggaggga gaactgccgg aggctgaacg acagattgct
     2101 tttctgcgta tgggaatgga tatcaatgcc tggctggttt cccgctcact gtggaatgct
     2161 gagcagtctc aggatgttga gacgctttga gcatccatta tgacaacatg gtcgtatgat
     2221 gcgctgggcg cgggggcgga gagggttctg tcgctgagcg gtatggggac cattgagaat
     2281 gccggggatg atgatcatga ggcgctgacg ccggaaaagt cctgacgcgg gaaatgcagt
     2341 ttgtcatgcg gcttgcccgg gagttccggc gggcagactg gcggcggatg ctgtcggaaa
     2401 tgtcggccac tgagcttggt gagggggcga ttatttccgg atgcagagct tcagtgatgt
     2461 gtggatggat gcgcagtttg cctcgctgaa ggcattgatc gtgagaatgg tgtccggcag
     2521 cagtgatgct gcggtggctg atttcagcct tttaccggaa gagaacggga taccggagcg
     2581 aacggacgaa gaactgatgc atcttgggga aggtatttcc ggaggtgtgc gttatggacc
     2641 agatagccaa cctggtcatt gatttgggga ttgatgcggc agagtttaaa aatgaaattc
     2701 cccgtatcaa aaaccttctg aatggtgcag ccggtgatgc agaacggtct tctgcccgta
     2761 tgcagcgttt tatggagcgt cagactcagg cggcccggca gacaatgcat gcggcttctt
     2821 cggctgcaac agcagcatca gcccatgcgc agacggtgga gaagaatgca cgggctcatg
     2881 aacgcatggc ccgtgaggtg gaacaaaccc gtctgcgcgt ggatgccctg aatcagaaaa
     2941 tgcgcgagga acaggcgcag gccagggcac tggcggaggc gcaggataaa gcggctgccg
     3001 ccttttatcg ccagattgac agtgtgaaac aggccggtgc ggggcttcag gaattacagc
     3061 gtattcagca gcagatccga caggccagaa acagtggcgg ggttggtcag caggattatc
     3121 tggcgctgat ttcggagatc acggcgaaaa cccgtgccct gacgcaggca gaggaacagg
     3181 ccacccggca gaaagcggcg tttatccgcc agcttaaaga gcaggcaacc cgccagaatc
     3241 tgtcgtcttc tgagttgctt cgcgccaggg cggctcagct gggggtaagc agtgctgcag
     3301 aagtgtatat ccgcaaaatg gagcgggcag gaaaagccac acattcgctg gggctgaaaa
     3361 gtgcggcagc ccggcgggag ctgggggtgt taatcagtca gatggcgcgc ggcaattttg
     3421 gtgcgctgag gggatccggg ataacgctgg ctaaccgtgc cggatggaca ggcgcactga
     3481 tgtcgccgaa aggcatgatg actggcggcg ttattggcgg acttgtcgcg gcggtcctgg
     3541 gtctgggtaa agcctggcat gacggccgga aggagggcga ggaatttaac cgtcagctgg
     3601 cgctgacggg acattatgcc ggtgtcactg tcgggcagtt gtggaaactc agccgggcca
     3661 tatccgggaa tggtatcacg caacatgcgg cagccggtgc gctggcacag gtggtgggca
     3721 gtggggcatt tcatggaaac gatattggta tggtggcgaa agctgccgca cagatggagc
     3781 gatcggttgg ccagtcggtc agcgatacca taaatcagtt taagcggctg aaggatgatc
     3841 ctgtaaatgc cgcgaaggcg ctggacaatg cgctgcattt tttgactgcc actcagcttg
     3901 agcagatacg cgtccttggg gagcaggggc ggtccagtga tgcggcccgg atcgccatgt
     3961 ctgcgctggc agaggaaacc ggtaaacgca catctgatat tgataataat ctcaatgcgc
     4021 tgggtagtac gctgcaaacc ttgtctgact ggtggaagca gttctgggat gcggccatga
     4081 atattggtcg tgaagactcg ctggatgcgc agattgatgc gttacaggaa aaaattcagc
     4141 gcgcgaaaaa atatccgtgg acaaacgcct ccacacaggt ggagtacgat cagcagcgtc
     4201 ttaacgatct tcaggagaaa aaacgccgga aggatttgca ggatgcaaaa gcgcaggcag
     4261 aacggaatta ccaggagcaa cagaaacgcc ggaatgctga aaatgccgcg ctgaaccgga
     4321 tgaatgaaac ggaagctgca cgacatcagc gggaaattgc gcgtattaat gccatgcagt
     4381 acgccgacca ggctgtcagg gatgcggcga tacagcgtga aaatgaacgt tacgaaaaag
     4441 ccattaagaa aaatacacgg gcaacccgta atgatgaggc cacccggtta ttgctgcagt
     4501 acagtcagca gcaggcacag gtggaagggc agattgctgc tgccagacag tcagcaggca
     4561 ttgccactga aaggatgact gaagcgcata aacagcttct ggccctgcag cagcgcatca
     4621 gcgacctgga cgggaaaaag ctgacggcag atgaaaagag tgtgctggcc cgtaagaatg
     4681 agctgattca ggcgctgacg ctgctggatg tgaaacagca ggagctgcag aaacagacag
     4741 cgcttaacga cctgagaaaa aaaacggttc agctgaccag ccagctggca gacaaagaac
     4801 gtgcactgcg tgagcagcac aatctggata ttgccactgc aggtatgggg gataagcagc
     4861 ggcagcgcta ccaggcacag ttgcgcatcc ggcaggaata ccggcaacag ttgcaacagc
     4921 ttgagaatga cagtcgccag aaaggcactt acgggacgga ggactaccgg agggctgagg
     4981 aggtgctgaa ggggagcctg aagcgacaac tgaatgaaaa caaacgctac tggcaggaac
     5041 tggaagtggc gcagggcgac tggaaaaacg gtgccatgcg ggcgtttcag aattttacgg
     5101 cggatgcgga taatgcggtg ggaacggcag aacagatgtt tacagtggca ttcagcagtg
     5161 ccggtaatgc actggcgaca ttctgtacca ccggtaagct gaattttaaa tccttcacct
     5221 cttccctgtt gtcagatatg gccagaatta tggcacagat ggccatgatg caggcggtaa
     5281 agggcgtcgg ctctttattc ggcttcacga ctaatgctga tggcggtgtt taccagtctg
     5341 ctgatttgag tcgctacagt ggcacggtgg ttaaccgtcc gacgtttttt gcttttgcaa
     5401 aaggcgcggg tgtgatgggg gaagcgggac ctgaagccat tctgccactg cgtcgcggtg
     5461 ctgatggtaa gctgggggtt gtggcggata ctggtggttc aggcatggtg atgtttgccc
     5521 cgcagtacaa catcgggatc aataacgacg gcacgaacgg gcagataggt ccggcagcac
     5581 tgaaggtggt ttatgacctc gggaaaaaag cggccgcgga ctttatgcaa cagcaggccc
     5641 gtgatggtgg ccggttaagt ggagcatacc ggtaatggag acgtttcgct ggaaggtgcg
     5701 cccggatatg aatgtggtat cagagccaaa agtggtggca gtgaagctgg gtgatggtta
     5761 tgaacagcgt cgcccggcgg gactgaataa tctgctgtcg acttacagcg tgacgatacg
     5821 tgttcgtaaa ggtgaacacc cacctttaaa agcctttctg gaacggcacg gtggcgtccg
     5881 cgcatttcag tggacgccac cttatgactg gaaaccgatc agggtggctt gtcgtaaatg
     5941 gtcggcaagc gtgggggcgt tatgggtgac tgtaacggcc ga
//
LOCUS       DAJVOY010000208         5961 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVOY010000208 DAJVOY010000000
VERSION     DAJVOY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.210"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(17..385)
                     /locus_tag="OQL90_003690"
     CDS             complement(17..385)
                     /locus_tag="OQL90_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370311.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            911..1606
                     /gene="minC"
                     /locus_tag="OQL90_003691"
     CDS             911..1606
                     /gene="minC"
                     /locus_tag="OQL90_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1370312.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1630..2442
                     /gene="minD"
                     /locus_tag="OQL90_003692"
     CDS             1630..2442
                     /gene="minD"
                     /locus_tag="OQL90_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1370313.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2446..2712
                     /gene="minE"
                     /locus_tag="OQL90_003693"
     CDS             2446..2712
                     /gene="minE"
                     /locus_tag="OQL90_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1370314.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3191..3310)
                     /locus_tag="OQL90_003694"
     CDS             complement(3191..3310)
                     /locus_tag="OQL90_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1370315.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3271..3456)
                     /locus_tag="OQL90_003695"
     CDS             complement(3271..3456)
                     /locus_tag="OQL90_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370316.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3557..3730
                     /locus_tag="OQL90_003696"
     CDS             3557..3730
                     /locus_tag="OQL90_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370317.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3732..4076
                     /locus_tag="OQL90_003697"
     CDS             3732..4076
                     /locus_tag="OQL90_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1370318.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4080..4415
                     /locus_tag="OQL90_003698"
     CDS             4080..4415
                     /locus_tag="OQL90_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1370319.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4472..>5961)
                     /locus_tag="OQL90_003699"
     CDS             complement(4472..>5961)
                     /locus_tag="OQL90_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1370320.1"
                     /translation="TTTLKLSENTIWNMKDDSVVTHLTNSDSIINLSYDDGQTFTQGK
                     TLTVKGNYVGNNGQLNIRTVLGDDKSATDRLIVEGNTSGSTTVYVKNAGGSGAATLNG
                     IELITVNGDESPAEAFRQGDARIAAGAFEYQLKQQGKNWYLTSYQSVNEEDNSSEGNS
                     EPTETPTPVLRPEAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQ
                     IGGHNAWRDSNGQLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSS
                     VSDYRSKGSVRGYCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSA
                     KGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDA
                     NNNIQTRLGFRTFIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGAS
                     NLGEIKLGVNGNLNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1632 a         1379 c         1368 g         1582 t
ORIGIN      
        1 taatgtaaac aaggcatcac tcacgacaca taaataacat ctttgtggtg atttgattga
       61 tttttccact ccgcttacca tccacaccaa aataacgatc atcacgacat aatttttctt
      121 taacatcgca aaatacaccg ttagcgaatg taaattcgct gggatcgtag ccttgtaatg
      181 attgtaagtt ttcagcggct ttttcgccga gatatttcct tgttaactct ggggaaatac
      241 catctgagtt cacacaaata tacgggtcgc aaacaacgcc gcacgccgga gattgaagtc
      301 cgccagcgtc atgtccagac gccattgatg agaacatcat ccccacacct gacaatccga
      361 tataaaaaat acgcatcata ttcatagcac atcctgataa tccaaccgta caacacttat
      421 gctcatttca tcatcaatcc gtaatcaaaa ccgctgacct ggacgagtta cgatgacctg
      481 acagaagtat cagacgtctt ctgcggtttc tatgattatg atagagcaat tctatggcgg
      541 atattaagat aacgggtatc tgttctttaa tgatgctgca aaaaatggaa agttaagaaa
      601 gcctggcgtg gtgaaaaaat acacttgcct caatactgga tatttaaaca ggtctggaga
      661 tgctgtcacg attagggtgc ggaaaaagga agcgtaaata agaacaatca aaagtacgac
      721 ggcaatgggt tgattgacaa gggtattttt taagctatga atcagcgcca tttatcacag
      781 aatagacttt tactctgaat aaatgggagg gtgacttgcc tcaatataat ccagactata
      841 acatgcctta tagtcttcgg aacatcatcg cgcgctggcg atgattaata gctaattgag
      901 taaggccagg atgtcaaaca cgccaatcga gcttaaaggc agtagcttca ctttatctgt
      961 ggttcatctg catgaggcag aacctaaggt tatccatcag gcgctggaag acaaaatcgc
     1021 tcaggccccc gcatttttaa aacatgcccc cgttgtactc aacgtcagtg cactggaaga
     1081 cccggtaaac tggtcagcga tgcataaggc ggtgtcggca accggtttgc gggttattgg
     1141 cgtaagtggc tgcaaagatg cgcaacttaa agccgaaatt gaaaagatgg ggctgcctat
     1201 cctgacggaa ggaaaggaaa aagcgccgcg tccagctccc gcaccacagg ctccagcgca
     1261 aaatacaacg ccggtcacaa aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg
     1321 tatttatgct ccacaatgtg atctgattgt tacaagccac gttagcgctg gggccgaatt
     1381 gattgccgat gggaacattc atgtctatgg catgatgcgc ggtcgtgcgc ttgcaggggc
     1441 aagtggtgac cgggaaacgc aaatattttg tacgaacctg atggcggaac tggtgtccat
     1501 cgcaggtgaa tactggctga gtgatcaaat cccagcagaa ttttatggca aagcggcgcg
     1561 actgcagtta gtcgaaaacg ctttgaccgt tcaaccgtta aattgatccc tttttaacaa
     1621 ggaatttcta tggcacgcat tattgttgtt acttcgggca aagggggtgt tggtaagaca
     1681 acctccagcg cggccatcgc cactggtttg gcccagaagg gaaagaaaac tgtcgtgata
     1741 gattttgata tcggcctgcg taatctcgac ctgattatgg gttgtgaacg ccgggtcgtt
     1801 tacgatttcg tcaacgtcat tcagggcgat gcaacgctaa atcaggcgtt aattaaagat
     1861 aagcgtactg aaaatctcta tattcttccg gcatcgcaaa cacgcgataa agatgccctc
     1921 acccgtgaag gggtcgccaa agttcttgat gatctgaaag cgatggattt tgaatttatc
     1981 gtttgtgact ccccggcagg gattgaaacc ggtgcgttaa tggcactcta ttttgcagac
     2041 gaagccatta ttaccaccaa cccggaagtc tcctcagtac gcgattctga ccgtatttta
     2101 ggcattctgg cgtcgaaatc acgccgcgca gaaaatggcg aagagcctat taaagagcac
     2161 ctgctgttaa cgcgctataa cccaggccgc gtaagcagag gtgacatgct gagcatggaa
     2221 gatgtgctgg agatcctgcg catcaaactc gtcggcgtga tcccagagga tcaatcagtg
     2281 ttgcgcgcct ctaaccaggg tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa
     2341 gcctacgcag ataccgtaga acgtctgttg ggagaagaac gtcctttccg cttcattgaa
     2401 gaagagaaga aaggcttcct caaacgcttg ttcggaggat aagttatggc attactcgat
     2461 ttctttctct cgcggaagaa aaacacagcc aacattgcaa aagaacggct gcagattatt
     2521 gttgctgaac gccgtcgcag cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat
     2581 attcttgagg tcatttgtaa atacgtacaa attgatcctg agatggtaac cgtacagctt
     2641 gagcaaaaag atggcgatat ttctattctt gagctgaacg tgaccttacc ggaagcagaa
     2701 gagctgaaat aagcccgctg taaaaacgca tttatcttca aggcagagtc atttctgcct
     2761 tgagtttttc atccctctca tccacgttgt ggtaaagcgg cgagtattct tgctgatact
     2821 cctcattgct atttccacct ccccctttac accttaaggc tgttcccacg cacagaacaa
     2881 aatgtttatg cctggttgag taaataacct cattgttagt gtggatacac tgggggatga
     2941 taaccccgca atttcattat tcattgttct cccataggat gaggtttccc gccgtaactg
     3001 gcgggctttt tttgccccag aattttccct tttagccccc tgtaaacgaa aactgcgccg
     3061 aagcgcagtt taatcgttga tggtcagaac gaccagttca cgatatatac tgcgggtatc
     3121 gactgatttc gattccatgc cattggataa atgatagtag cgtctaagtg acctaagctc
     3181 tgctgcaaca tcagcccgca ggcaccagac caggggattc atcctgaaga gacagcgcag
     3241 gtgtattgtg ttcaccactc accaaagaca tcatgatgaa atgatgatat cccgcataag
     3301 aaagaggcat tttttaaacg cagtgcgctg aagtgtagtt ggataaaaaa tcactctttt
     3361 cagataatac tgtcgtacgc ttatctttcg acaatgaggc cgtcggcaaa ataaaatgac
     3421 taatgtaatc gttgctgatg aatatcttct gctcacataa aaatcacaca ataatattga
     3481 taaatcagat tatcttacct ttacaacatt gcgttaacag tcaatgacac ttcttgaata
     3541 aaaatattgc caatcaatga gttattcctc atttaagatt attctcattc acgtaaaaaa
     3601 cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata gcgagcgttc
     3661 tttagttaag cagatcttaa ttgaagacga aattattgtg cgtgcaactt acttaatacc
     3721 ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct
     3781 ccacgccagc gatggcggat atgaatcgca ccacgaaagg tgcattgcta ggcgcaggcg
     3841 ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg
     3901 gtgttggtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag
     3961 taggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga
     4021 ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa
     4081 tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca atgacctggg
     4141 cgcaggacta caatattaaa aacggtttac cgtcagaaac ctatattact tgcgcagaag
     4201 ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta
     4261 atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag
     4321 tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg atcacggcaa
     4381 ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc gcgtcgatga
     4441 atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg cccaccatca
     4501 ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc cagacgctgg
     4561 ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta ctcgccccat
     4621 cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta tggatccagt
     4681 tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc tcctgagtac
     4741 gaataaaggt gcggaaacca agtcgggtct ggatgttgtt atttgcgtca ttctcaatac
     4801 gtgagccatt ggcttcggtg tgcgcattat gatcgacgcc catccagata gcctgtgcct
     4861 gtggctggaa tatccacgtg tatttcgccg cttccagacc aaaggattta ttcagggcaa
     4921 agccgtaacc cgcttccagc gagacggttg cgcctttcgc actataggat tcataggcta
     4981 attcatcccc tttcaccgag tttttaaacc agctatattg cgcccaggcg tcaatgtatg
     5041 cgcctttttt actgatgtca tcggcaaacc aagtggcata cagccctgcg caatagcctc
     5101 tgacactccc tttcgaacga taatccgaca cgctggaatg agttgagttg tagtcgcggg
     5161 cataaccagc catcactccc aaacgccaac tgtcactgtc ggtaaaacca ccggttaaca
     5221 gatcggcccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt ccgttgctgt
     5281 cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa cgctcctgtt
     5341 cagttacagg atcgatgtag cgcatttcac ccgcacggtc gttcagacgc ataacaaaaa
     5401 gagtgttagc ggctgccagg ttggcgacgt aacttccagc ttcggggcgc aggacggggg
     5461 taggcgtttc tgttggttct gaatttccct ctgagctgtt atcctcctca ttcactgact
     5521 gataacttgt caaataccag tttttgcctt gttgttttag ttgatattcg aaagctccag
     5581 ctgcaatcct tgcatcacct tgtctgaagg cttctgctgg agattcatcg ccattcacag
     5641 ttatgagttc gatcccgttt agcgtggccg cgccgcttcc tccagcattt ttcacataga
     5701 cggtagttga acccgaagta ttaccctcaa caataagtct gtccgtagcc gatttatcat
     5761 cacctaatac ggtgcggata ttaagctgac cattattacc gacataatta ccttttacgg
     5821 ttaatgtttt tccttgggta aatgtttgac catcatcata cgaaagattg ataatactgt
     5881 ctgaattagt cagatgggta acaacggagt catctttcat attccagatt gtattttctg
     5941 aaagcttcag cgtggttgtg c
//
LOCUS       DAJVOY010000209         5934 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVOY010000209 DAJVOY010000000
VERSION     DAJVOY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.211"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..2090)
                     /locus_tag="OQL90_003700"
     CDS             complement(<1..2090)
                     /locus_tag="OQL90_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1370321.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPSDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAVGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIA"
     gene            2332..3393
                     /locus_tag="OQL90_003701"
     CDS             2332..3393
                     /locus_tag="OQL90_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCS1370322.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3506..5005)
                     /gene="ansP"
                     /locus_tag="OQL90_003702"
     CDS             complement(3506..5005)
                     /gene="ansP"
                     /locus_tag="OQL90_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCS1370323.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5272..5889
                     /locus_tag="OQL90_003703"
     CDS             5272..5889
                     /locus_tag="OQL90_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCS1370324.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLTLENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1569 a         1510 c         1425 g         1430 t
ORIGIN      
        1 cacgcaatat tcatgccgac accataattt cgtccgggcg aaggttcgta atatcgcccg
       61 tttgactcat tgacaatgac agaaccaacg tattctttat cgaataaatt atcgacacga
      121 ccaaataaat ccacagttaa attgtggtaa ttgtatttat acccggtgaa taagccgacg
      181 agagtataag acggcgcttt tgccgtattt tcatcatctg ccataatatc gcccatataa
      241 cgcgcttctg tgcctgcata ccaaccatct tccggtacat aacctatcga cgcaaagccc
      301 atattacggg cgatccctgg catccgatta ccgttacaat cctgttcatt gcaaacattg
      361 ctgcgatagg tcgcatcaag ccaggtccag gacgcgttta cgcgaaaatc tcctgcgaaa
      421 cgttgatccc atgccagttc agcgccttga cgacgggtct ttccggcatt tttgtaagtc
      481 gtacgcccac cgctactgct atcgacaaca atttcatcat cagtgtcggt ctgaaacaat
      541 gcgagactaa gcagcccatc accaatacgc gttttactgc cgatctcaat tgtatcgttg
      601 gtggatggtt ttaaaccaaa gttcataccg ctttgcccat cagcacgata agacagctca
      661 ttaatcgtcg gcgtttcaaa acctcgcccg actgccagat agatattcca ggcatcggtc
      721 attgcatatt ttaacgaacc ggcaggtagc catttatgat aactggcatc accgctgtca
      781 tcgccgttac ccggagtaac gtaatggtcg ttggaatcaa accacacgga gctgtagcgc
      841 acgccagcat ccagcgacag tttttcgctc agctgccact gcgtctgtaa atagggatcg
      901 atgttccaca tcagattgcg ttcgtcgcga cgcaactcac ctttttgccc gtactccggc
      961 atgccgctat tcaggcggaa gttattgtag cccttgcggt tttcactcat gttttcgtag
     1021 ttcaggccgg tagtgaacgt gaccggaacg cccagttcac cacggtgtgt ccagcggctg
     1081 tctatcccct ggtaatggcg ttgcagggta atcacgccgc ccgcatgtga cgggttaagt
     1141 tgtggtgcca tgggtattga ctggtactgg gtcgtttctc gctctccggc atacatcatc
     1201 acactcatat catcttgcgc gctcaggcta cgctcatagc gcaaccctgc ctgagtttgc
     1261 ttgatggttt ttcgcgtgtc gtactgttct gcacgaggcg cttgttgtgg attagccttc
     1321 cattctgctt tggttagccc acctgggtca tctgctttga tatccacact attgaaaatc
     1381 agacttaatt tgctggcttc atcaatgcgt acgcccagtt tggcattggc taaatttttc
     1441 tgtgcgccac tatggtcacg atagccgtgg gtcgtaaaac gcgtggttga gacggtgtaa
     1501 tcgacatcgc taggctgtgt gccgtctccc gttgcgcccg ttgctttcag cccatagcgc
     1561 cagctgccaa aactgccgta gtaactactg gcttcaatgg ttggtggctg ttgtccggtc
     1621 tgggtggtga cattcattac cccaccagac gcgttgccat acagggcaga gaaggggcca
     1681 cgcagcactt ccacattttg cacactgctt aaatcgatgt tggatgtttg cccttgcccg
     1741 tcgggcatgg tggcgggaat accgtccaca tacaggcgaa taccgcgaat accgtaagtg
     1801 gagcgggagc caaatccgcg aatcgacagc tgtaaatctt gcgcatagtt ctgccggttt
     1861 tgtacctgca aaccaggcac gccggtcagt gattcggaca agttaatgcg cggtgttgcc
     1921 aggcgcatct cctcgccatc caccacgctt actgctgctg gggtatccag ttctgaaacc
     1981 acctgcggtg cggcactgac aatcatagtc tgttcatcag cggcaaaaac aacgggggaa
     2041 aggacaagca gtgcgggcaa aacggtctgt cggacggaaa aaatcttcat gaaaaaagcc
     2101 aggttaagaa tgggaaaacg ctgtcatggt aataaaattg taaacttatg caaaacgaaa
     2161 cggcacgata cgttaattaa ttgagaaaat tgtagtcgta acggcaagaa atgctccaca
     2221 tttgagaaaa taatgattac cattcccatt tataacaaga gcgtgacgat gattacgctt
     2281 agcgaagcat tgtgaagcag caaaaatatt ggttcatcaa agggagtcgt catgcattta
     2341 cgtcatctgt tttcatcgcg cctgcgtggt tcattactgt taggttcatt gcttgttgct
     2401 tcatcattca gtacgcaggc cgcagaagaa atgctgcgta aagcggtagg taaaggtgcc
     2461 tacgaaatgg cttatagcca gcaagaaaac gcgctgtggc tcgccacttc gcaaagccgc
     2521 aaactggata aaggtggcgt ggtttatcgt cttgatccgg tcactctgga agtgacgcag
     2581 gcgatccata acgatctcaa gccgtttggt gccaccatca ataacacgac tcagacgttg
     2641 tggtttggta acaccgtaaa cagcgcggtc acggcgatag atgccaaaac gggcgaagtg
     2701 aaaggccgtc tggtgctgga tgatcgtaag cgcacggaag aggtgcgccc gctgcaaccg
     2761 cgtgagctgg tagctgatga tgccacgaac accgtttaca tcagtggtat tggtaaagag
     2821 agcgtgattt gggtcgttga tggcgagaat atcaaactga aaaccgccat ccagaacacc
     2881 ggtaaaatga gtaccggtct ggcgcttgat agcaaaggca aacgtcttta caccactaac
     2941 gctgacggcg aattgattac catcgacacc gccgacaata aaatcctcag ccgtaaaaag
     3001 ctgctggatg acggcaaaga gcacttcttt atcaacatca gccttgatac cgccaggcag
     3061 cgtgcattta tcaccgattc taaagcggca gaagtgttag tggtcgatac ccgtaatggc
     3121 aatattctgg cgaaggttgc ggcaccggaa tcactggctg tgctgtttaa ccccgcgcgt
     3181 aatgaagcct acgtaacgca tcgtcaggca ggtaaagtca gtgtgattga cgcgaaaagc
     3241 tataaagtgg tgaaaacgtt cgatacgccg actcatccaa acagcctggc gctgtctgcc
     3301 gatggcaaaa cgctgtatgt cagtgtgaaa caaaaatcca ctaaacagca ggaagctacc
     3361 cagccagacg atgtgattcg tattgcgctg taatagatat ggcgagggtg aaaacggtga
     3421 gacttgcctg ccttttatca cggcgtatcc ggcactttcg tcagtaatct gaatttgccc
     3481 ggcacgtagc cgggcaattt tgcaattaac tcgccgtttc aggcttaaac acaatttcct
     3541 gtttttcttc atcttcctcg acgactggcg cagtgctgtg aatttcagca acgcgtttgc
     3601 gcacaccaaa ccagcctata accagcagaa taccgataat cggcagcgcc gcgatagtat
     3661 aagtcccgtt cgggtaatcg aacgccatca gcacaaggac actcagtaaa aacagtaatg
     3721 tcagccagga agtgaagggc gcgccaggca gtttaaaact gacatccgct gctttgcctt
     3781 ctttaatcgc tttacgcagg cgcatctggc acacgatgat aaacgcccat gaagcgatga
     3841 ttcccagcga cgcgaagttc aacacaatct caaatacgcg cgacggcacc agatagttga
     3901 ggaatacgcc gacgacatac acaaccagtg tcgctagaat cccggcatac ggcacatgct
     3961 gacgactcat tttcgccata aaactcggtg cggaaccgcc catcgccatt gagcgcagaa
     4021 tacgtccggt gcagtacaga cctgagttca ggctggagag ggcagcggtc agcaccacaa
     4081 tgttcataat gctgccgata tatggcacac ccagtttaga gaaaaacgtc acgaacggac
     4141 tttgccccgc ctgatacgcg ctccacggca ataacataac cagcaacacc acggagccga
     4201 cgtaaaacag gccaatacgc caaatcacac tgttaatggc tttaggcacc atggtctgcg
     4261 gatctttaca ttctccggca gctgtaccca ccatttcaat ggaggcaaaa gcaaacacta
     4321 cgccctgaat caacaccagc gcaggcagca aaccgtgggg gaagaagccg ccattatcgg
     4381 tgattaaatg aaagccagtg gtgttgccat ccagcggctg accactaccg aggaacactg
     4441 tacccacgac caaaaaggtc acaatggcga gcactttaat aagcgcaaac cagaactcca
     4501 tctccgcaaa ccatttcaca ccgatcatat tcatggtgcc aacgatggta agtgcagcga
     4561 gcgcaaagac ccactgcggc acgccgccaa acgcacccca gtaatgcata tacagagcga
     4621 cggcggtaat atcaacaatc cccgtcatcg cccagttgat gaagtacatc cagccagcaa
     4681 cataagcggc tttctcaccc aaaaactcac gggcataaga gacaaaactg ccactggaag
     4741 ggcggtgtag caccagctca cccaatgcac gcagaataaa aaacgaaaac aagccacaaa
     4801 ttaaataaac cagtgccagt gctggacccg ccatttgcag tcgggctcct gcacctaaaa
     4861 acaagccggt gccaatcgcg ccgccaatgg cgatcatctg tacctggcga ttgcccatcg
     4921 ctttgtgata cccctcttcg tgggcattaa gccagcggcg tttcgcggcg tgttgatctg
     4981 aagtgtcggt gtcgtgttta ctcattgctc tccctgattg ctttaatgaa aaagtcatat
     5041 aagttgccat gaacaatgtt tattcatcct ggcgatgata gttatcacca ttattcttta
     5101 tgggtataaa actatcggcg cagcatccta cccgttatcg atatacgatg caaaacatcc
     5161 ccttacaatc ctgaagggga ttcatacaac tgacgaaaaa atgacaaata cttttgctgg
     5221 ttaacctgtg tactgtccta cacttaatct ttaaaagatt gtgaggggca tatgattaag
     5281 gtgtatggcg tacccggatg gggctctaca atcagtgagc tgatgctaac actggctgat
     5341 attccttatc agttcgttga tgtcagtggt tttgaccacg agggaacttc acgcgagtta
     5401 ttgaaaaccc tgaacccgtt gtgtcagatt ccgacactga cgctggaaaa tgacgagatt
     5461 atgacggaga cagcggcgat tgcattgatg gttctcgatc gtcgcccgga ccttgcgcca
     5521 ccggttgggc gcgccgaacg tcagcagttt caacggctat tggtctggct ggttgctaat
     5581 gtctatccaa cattcacttt cgccgattac cctgagcgtt gggctcctga cgcacctgaa
     5641 cagttaaaga agaatgttat tgaatatcgt aaatcgcttt atatctggct gaattcacaa
     5701 ttgaccgctg aaccttatgc gtttggtgag caactaacgc tggtggactg ttatctttgc
     5761 actatgcgca catgggggcc tgggcatgaa tggtttcagg ataatgccac gaatatcagt
     5821 gcgattgctg atgctgtatg tcagttaccg aaattacaag aagtattaaa aaggaatgaa
     5881 attatttgag tgggatacca gaggattatg ttatttatta ttcagtaact ttat
//
LOCUS       DAJVOY010000210         5883 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVOY010000210 DAJVOY010000000
VERSION     DAJVOY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.212"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1264
                     /locus_tag="OQL90_003704"
     CDS             <1..1264
                     /locus_tag="OQL90_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1370325.1"
                     /translation="ADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLETVLA
                     AMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALAR
                     RKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFPSTMVDRIV
                     PAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPY
                     EEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQGVD
                     LQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVA
                     GWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNS
                     LFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1300..1503)
                     /gene="ydfZ"
                     /locus_tag="OQL90_003705"
     CDS             complement(1300..1503)
                     /gene="ydfZ"
                     /locus_tag="OQL90_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1370326.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1681..2365)
                     /gene="ydfH"
                     /locus_tag="OQL90_003706"
                     /pseudo
     CDS             complement(1681..2365)
                     /gene="ydfH"
                     /locus_tag="OQL90_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
     gene            complement(2454..3200)
                     /gene="ydfG"
                     /locus_tag="OQL90_003707"
     CDS             complement(2454..3200)
                     /gene="ydfG"
                     /locus_tag="OQL90_003707"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1370327.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3337..5382
                     /gene="dcp"
                     /locus_tag="OQL90_003708"
     CDS             3337..5382
                     /gene="dcp"
                     /locus_tag="OQL90_003708"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1370328.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5427..>5883)
                     /locus_tag="OQL90_003709"
     CDS             complement(5427..>5883)
                     /locus_tag="OQL90_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS1370329.1"
                     /translation="SALSQRLIADQLRLTTAESCTGGKLASALCAAEDTPKFYGAGFV
                     TFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAIERADADVSIAITGYGGLEGGE
                     DGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAVRFAHVQLLQLLL"
BASE COUNT         1457 a         1424 c         1526 g         1476 t
ORIGIN      
        1 tgccgattta caacagcaag ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg
       61 gacggctcgc gtcgttggcg tcgttaaaaa agccttgcac gtacagatgg atggcttaga
      121 aaccgtgttg gctgcgatgt gtgaaccgca aatcgcgatt gtctctctga caatcaccga
      181 aaaagggtat ttccactctc cggcgaccgg acagttaatg ctcgatcacc cgatggtcgc
      241 tgccgacgtg caaaatcccc accagccgaa aacagcaaca ggggtgattg ttgaggcgct
      301 ggcccgccgt aaagcggcag gacttcccgc atttaccgtc atgtcatgtg acaacatgcc
      361 agaaaacggt catgttatgc gtgacgttgt cacttcatac gcgaaagctg ttgatgtaaa
      421 actggcacaa tggatcgaag ataacgtgac tttcccatca acaatggtgg accgtattgt
      481 gcccgcagtg acagaggata cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc
      541 tgcgggcgtt gcctgtgaac ctttccgcca gtgggtaata gaagataact ttgttgccgg
      601 acgtccagaa tgggaaaaag cgggagccga actggttagt gatgtgctgc cttatgaaga
      661 gatgaagttg cgcatgctca acggcagtca ttcattcctg gcgtatttgg gttatcttgc
      721 cggatatcag cacattaatg actgcatgga agatgaacat tatcgttatg cggcgtatgg
      781 cttgatgttg caggaacaag cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta
      841 cgctaaccga ttaattgaac gctatagcaa tccggcgtta cgtcatcgaa cctggcagat
      901 tgcgatggat ggtagccaga aattgccaca gcggatgttg gattctgttc gctggcatct
      961 ggcgcatgac agcaagttcg atctgctggc gctgggcgtc gcgggttgga tgcgttatgt
     1021 cggtggtgtt gatgaacagg gaaatccgat agaaatcagt gacccactgt tacctgttat
     1081 tcagaaggct gtacaaagta gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat
     1141 taaggcaatc tttggtgatg atttgccaga caatagcttg tttactgcaa aagtgacgga
     1201 agcgtacttg tctttattag cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa
     1261 gtaaaaaaag caatgccgcc atcggcggca ttcacacact tagttcatgc cgagacgaat
     1321 caggtccaga ggtgccagtt tctcttcaca accttcaaca actacggttt ttccgcggcg
     1381 gatttgctca gcggtcagac cttcagtatc aatcgacagg atcttgccag tgtgaccggt
     1441 gccgctaacc ataacacggc tgccagtggt gattgcgtta cggttacgat cgtaggtcgt
     1501 cataggtgtt ttctcctttc tgatttacag tcgaagccca cgtaacgggc gcaatataaa
     1561 tactcctgtc gagtgaaata ttttttgttt ttgatcaagc tcacatcttt tttgatgcat
     1621 gcatgagccg taaggcgggg ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa
     1681 ttactcttcg ctaaaccagt cgctgttttc ctggcggatc tggccacgga ttcgctgatt
     1741 tcctgcaaat gttgcgtcat tgcacgttct accgcatcgc catcacgttt ttgcagggca
     1801 gagaaaatat caagatgctg gcgtaacagc atttctggtg gagaaacatg gtcgaaactc
     1861 atatagcgca cgcgatcaac ggtcgctttc aggttctcaa tggtatccca cgccagttga
     1921 cagtcggcaa tctgcgtcag gagttgatgg aagttgtcat caagttcaaa aaatcatcca
     1981 gttgcttgtg ctcaatggca atgcgttgct ggtgaagatt ttgttccagt tggtagcact
     2041 ggctttcggt aatcatgctc gccgcccgac gcgccaccgc gcactcaatg gcctgacgga
     2101 taaaactgcc gttgcgcacc tgggccatgg aaattttgtt gacgtagctg ccacgctgtg
     2161 gacgaatttg aatcaggccg ttttccgcca gtttaataaa ggcttcacga accggctggc
     2221 gtgacacatt gaaacgaaca gaaacttctt tttccgacaa cggtgtgcct ggagcaatca
     2281 ggcaatggac aatgtcgcga cgaaggatac gataaatttg ctgattgaca ggctgtgtgg
     2341 gattaagttg cgtttcgacg gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt
     2401 ctaccacttt tttgtgacaa gcaataaccc ggcagttacg ccgggtataa aaattactga
     2461 cggtggacat tcagtccggc atagctttgg gtaaccggca tcatttccag ggtattgata
     2521 ttgacgtgag caggcagcgt tgacacccac caaacggctt cgctgacatc ttctggcgtc
     2581 aatgcaacgg tattttgata ggttttttcc gctttaccgt catcgccttt aaagcggaca
     2641 ttggaaaact cggtgccacc caccagaccc ggttcgatgt cggtgacgcg caccgccgta
     2701 ccatgcaggt ccgtacgcag attcaggcta aactgacgaa caaacgcttt cgtcgcaccg
     2761 taaacgttac caccggcata aggccagcta cctgccgttg agccaatgtt aataatatga
     2821 ccatgattac gttcaaccat acccggtaag acggcgcgcg tcatatatac caggcctttg
     2881 ttgttggtat caatcatcgt ttcccagtct tcaacgctgg ctttatgcgc aggctccatg
     2941 cccaacgcca ggccggcatt atttaccagg atatcaatat tgcaccactc ggcaggaagc
     3001 gatgccagca tctcttcaat agcggcgcgg ttgcgaacgt ccagttgggc gatgtacaga
     3061 ttatctccca gttcgtcttt taactcctgc aaccgttcct ggcgacggcc agtggcgata
     3121 actttatgcc cttgttgaat aaaacgacga gtaatgcatt caccaaaacc tgccgttgct
     3181 ccagttacta aaacgatcat ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc
     3241 acgtgatgct taagagcggg taatactggt ttgtaagatt gcactccgtt atcagtaagc
     3301 ctacgctatg gaacacctcg cgttcaggag aagaaaatga caacaatgaa tcctttcctt
     3361 gtgcaaagca cactaccgta tctggctccc cattttgatc aaattgccaa tcatcactat
     3421 cgcccggcat tcgatgaggg aatgcagcaa aagcgggcag aaattgctgc catcgcgctt
     3481 aacccgcaaa cgcctgattt caacaatact attctggcac tggaacaaag cggagaatta
     3541 cttacccgcg ttaccagcgt cttttttgcg atgactgcgg cgcataccaa tgatgaatta
     3601 cagcgtcttg atgaacagtt ttccgctgaa ctggcggaac tggctaatga tatctatctg
     3661 aacggtgaat tattcgcgcg ggtagatgct gtctggcagc gccgtgaatc cctggggctt
     3721 gatagtgaat ccatccgcct ggtggaggtg attcatcaac gttttgtcct tgccggagcc
     3781 aaacttgcgc aagctgataa agcaaaatta aaagtactga atacagaagc tgcgaccctg
     3841 accagccagt ttaaccagcg attactggca gcaaataaat ccggcggtct ggttgtgaac
     3901 gatatcgcgc agctggcagg aatgagtgag caagagattg cgctggcggc agaggcggct
     3961 cgcgagaaag gtctggataa caaatggctg attccgctgc tgaataccac ccaacaaccg
     4021 gcgcttgccg agatgcgcga tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg
     4081 cgagcggaaa aaaatgatgc caatgatacc cgcgctatca ttcaacgtct ggtagagatt
     4141 cgcgcacagc aggcgaaact gcttggtttt cctcattatg ccgcatggaa aatcgccgat
     4201 cagatggcaa aaacgccaga agcagcactc aactttatgc gggaaattgt tccagcggcg
     4261 cgtcaacgtg ctagcgatga attagcctcc atacaggcgg ttatcgataa gcagcaaggc
     4321 gggtttagcg cgcagccgtg ggactgggca ttttatgccg aacaggtacg gcgggagaaa
     4381 tttgatcttg atgaggcgca gctcaagcca tattttgaat taaacacggt gttgaatgaa
     4441 ggtgtattct ggaccgcgaa tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt
     4501 cctgtctacc atcctgacgt tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg
     4561 gcgttatttt acggtgattt cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc
     4621 aattttgttg agcaatcaac gcttaatgaa acgcatccgg taatttataa cgtttgcaat
     4681 tatcagaaac ccgctgccgg tgagcctgcg ttgttactct gggatgatgt cataacctta
     4741 ttccatgaat ttggtcatac gctgcacggc ctttttgccc gccagcgtta tgccacgctt
     4801 tccggcacca acacgccgcg tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg
     4861 gcaacgcatc cgcaggtatt cgctcgctac gcccggcatt atcagagcgg ggcagcaatg
     4921 cctgacgaac tgcaacagaa aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg
     4981 agcgaactgc ttagcgccgc acttctcgat atgcgctggc attgcctgga agaaaacgaa
     5041 gcaatgcagg atgtcgatga ttttgaattg cgggcgctgg tggcggaaaa tatggatctt
     5101 cctgctatac cgccacgcta tcgcagcagt tatttcgccc atatttttgg tggcggatat
     5161 gctgcaggtt attacgctta tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg
     5221 tttgttgagc agggcggatt aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt
     5281 tccagaggta acagcgaaga tctggaacgc ctgtatcgac aatggcgcgg taaggctcct
     5341 cagattatgc cgatgctgca acatcgtggc ttgaatatat aaacaccacc aatggctggc
     5401 ctggagatat cgccaggcca gcctggttat agcagtaatt gcagcagctg gacgtgggca
     5461 aacctcaccg ctaaagctaa taccgtttcg cagtcgccag caaaatgcat aaccgcagtg
     5521 taggtctggc ctttaatatg ccacgcgaac cagacggtac ccgctggcgt accatcttca
     5581 ccgccctcca gtccgccgta gccggtaatg gcaatactga catcagcatc cgcacgctct
     5641 atggcacctg ttgccatttc tgctgccact ttctcactca ccgcagaata tcgttcaaga
     5701 gattgctggc ttacgctgag gattttcatc tttgcctgat cggtgaaagt aacaaagcct
     5761 gcaccgtaaa atttgggtgt atcttcagct gcacacaggg cgctagccaa cttaccgccg
     5821 gtgcatgatt cggcggtagt taagcgtaat tgatccgcga taagtctttg actcaacgcg
     5881 gat
//
LOCUS       DAJVOY010000211         5875 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJVOY010000211 DAJVOY010000000
VERSION     DAJVOY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.213"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..368)
                     /gene="dsbG"
                     /locus_tag="OQL90_003710"
     CDS             complement(<1..368)
                     /gene="dsbG"
                     /locus_tag="OQL90_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1370330.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFA"
     gene            740..1303
                     /gene="ahpC"
                     /locus_tag="OQL90_003711"
     CDS             740..1303
                     /gene="ahpC"
                     /locus_tag="OQL90_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1370331.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1474..3039
                     /gene="ahpF"
                     /locus_tag="OQL90_003712"
     CDS             1474..3039
                     /gene="ahpF"
                     /locus_tag="OQL90_003712"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1370332.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3160..3588)
                     /gene="uspG"
                     /locus_tag="OQL90_003713"
     CDS             complement(3160..3588)
                     /gene="uspG"
                     /locus_tag="OQL90_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1370333.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            3809..5047
                     /locus_tag="OQL90_003714"
     CDS             3809..5047
                     /locus_tag="OQL90_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCS1370334.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            complement(5278..5688)
                     /gene="rnk"
                     /locus_tag="OQL90_003715"
     CDS             complement(5278..5688)
                     /gene="rnk"
                     /locus_tag="OQL90_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1370335.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
BASE COUNT         1514 a         1400 c         1523 g         1438 t
ORIGIN      
        1 tcggcgaaga cgtagacaat gaccggcgca tcttttttac cgtcgaggag ccagtgggat
       61 tgttccatcc gttgccacat ttcgcgtccg gctggtgcgt aaatttcttt ttcgataagt
      121 gtgttactca ggttttcacc tttctcgttg tacatgtaac cagagatagc gtgcttacca
      181 tctggagtca ggtagatggt gacgcccata tcctgatact ttccgagata acctttcatt
      241 cctccggggg catcgaacgt tttgatgatt gtaatgccct gtttttcaat cgcttttact
      301 ggagcaggaa gttcctctgc gaaggcgatt gcaggaagca gagccagtaa aagtatcttt
      361 tttaacatta atttgtcctt ttcagtcagt gcaaaagtcg agtaaaaggc ataacctatc
      421 actgtcatag gtaagagctt agatcaggtg attgcccttt gtttatgagg gtgttgtaat
      481 ccatgtcgtt gttgcatttg taagggcaac gcctcagcct gcaggcaggc gctgaagata
      541 ccaaagggta gttcagatta cacggtcacc tggaaagggg gccattttac tttttatcgc
      601 cgctggcggt gcaaagttca caaagttgtc ttacgaaggt tgtaaggtaa aacttatcga
      661 tttgataatg gaaacgcatt agccgaatcg gcaaaaattg gttaccttac atctcatcga
      721 aaacacggag gaagtataga tgtccttgat taacaccaaa attaaacctt ttaaaaacca
      781 ggcattcaaa aacggcgaat tcatcgaaat caccgaaaaa gataccgaag gccgctggag
      841 cgtcttcttc ttctacccgg ctgactttac tttcgtatgc ccgaccgaac tgggtgacgt
      901 tgctgaccac tacgaagaac tgcagaaact gggcgtagac gtatacgcag tatctaccga
      961 tactcacttc acccacaaag catggcacag cagctctgaa accatcgcta aaatcaaata
     1021 tgcgatgatc ggcgacccga ctggcgccct gacccgtaac ttcgacaaca tgcgtgaaga
     1081 tgaaggtctg gctgaccgtg cgaccttcgt tgttgacccg cagggtatca tccaggcaat
     1141 cgaagttacc gctgaaggca ttggccgtga cgcgtctgac ctgctgcgta aaatcaaagc
     1201 agcacagtac gtagcttctc acccaggtga agtttgcccg gctaaatgga aagaaggtga
     1261 agcaactctg gctccgtctc tggacctggt tggtaaaatc taaatttcct tcgtctttcg
     1321 cgctaccaac tccggcgcga aatacttcgg aaattcacct aattcttcgg gtgctgcggc
     1381 acccgatttt cttccccgca ctatgatgca agctgcatcc aggtagccgt agaggccgct
     1441 tgcatgatga tgtttaaagc ccaggagata aacatgctcg acacaaatat gaaaactcaa
     1501 ctcaaggctt accttgagaa attgaccaag cctgttgagt taattgccac gctggatgac
     1561 agcgctaaat cggcagaaat caaggaactg ttggctgaaa tcgcagaact gtcagacaaa
     1621 gtcaccttta aagaagataa cagcttgccg gtgcgtaagc cgtctttcct gatcaccaac
     1681 ccaggttcca accaggggcc acgttttgca ggctccccgc tgggccacga gttcacctcg
     1741 ctggtactgg cgttgctgtg gaccggtggt catccgtcga aagaagcgca gtctctgctg
     1801 gagcagattc gccatattga cggtgatttt gaattcgaaa cctattactc gctctcttgc
     1861 cataactgcc cggacgtggt gcaggcgctg aacctgatga gcgtactgaa cccgcgcatc
     1921 aagcacactg caattgacgg cggcaccttc cagaacgaaa tcaccgatcg caacgtgatg
     1981 ggcgttccgg cagtgttcgt aaacgggaaa gagtttggtc agggccgcat gacgttgact
     2041 gaaatcgttg ccaaaattga tacgggcgcg gaaaaacgtg cggcagaaga gctgaacaag
     2101 cgtgatgctt atgacgtatt aatcgtcggt tccggcccgg cgggtgcagc ggcagcaatt
     2161 tactccgcac gtaaaggcat ccgtaccggt ctgatgggcg aacgttttgg tggtcagatc
     2221 ctcgataccg ttgatatcga aaactacatt tctgtaccga agactgaagg gcagaagctg
     2281 gcaggcgcac tgaaagttca cgttgatgaa tacgacgttg atgtgatcga cagccagagc
     2341 gccagcaaac tgatcccagc agcagttgaa ggtggtctgc atcagattga aacagcttct
     2401 ggcgcggtac tgaaagcacg cagcattatc gtggcgaccg gtgcaaaatg gcgcaacatg
     2461 aacgttccgg gcgaagatca gtatcgcacc aaaggcgtga cctactgccc gcactgcgac
     2521 ggcccgctgt ttaaaggtaa acgcgtagcg gttatcggcg gcggtaactc cggcgtggaa
     2581 gcggcaattg acctggcggg tatcgttgag cacgtaacgc tgctggaatt tgcgccagaa
     2641 atgaaagccg accaggttct gcaggacaaa ctgcgcagcc tgaaaaacgt cgacattatt
     2701 ctgaatgcgc aaaccacgga agtgaaaggc gacggcagca aagtcgttgg tctggaatat
     2761 cgagatcgtg tcagcggcga tattcacaac atcgaactgg ccggtatttt cgtgcagatt
     2821 ggtctgctgc cgaacaccaa ctggctcgaa ggcgcagtcg aacgtaaccg catgggtgag
     2881 attatcattg atgcgaaatg cgaaaccaac gtcaaaggcg tattcgcagc gggtgactgt
     2941 acgacggttc cgtacaagca gatcatcatc gccactggcg aaggtgccaa agcctctctg
     3001 agtgcttttg actacctgat tcgcaccaaa actgcataag aagaagtaag attcacctgc
     3061 aattgcttag ccgccggggt caaacctagc ggctttttta tggcattaaa aagccctgcc
     3121 gggatgttcc aggcagggcg gcataaatac ttttatctgt tagcgaacga ccagtacagg
     3181 cagattggcg tggcggatca cgctcgaggc gttagaacct aacagatggg tcgaaatcga
     3241 tgggttgcga gaaccgataa ctacaacatc agcccccagt tcttctgcca actcattgac
     3301 ttcatcccgc acgctaccaa aacggacatg ttgtttaatg cgggaaggat cgatggtgaa
     3361 gtggctgacc atcgtttgca gacgttcttc tgcttcatgt tgcagatgct cttcaaaacg
     3421 acgcacatca gcggcaaaac ggtccaggct caggctggct gacccgggta gtacgtgaag
     3481 tagatgaata actccgtcat cctgggtgag gaattcagcg tggcgaacag ctttgtcgct
     3541 caattccatt tcaaatacat caactggcat aatgattgtc ttatacataa ccctttctcc
     3601 ctgttaatca tgaacaaatc attcgccatg attataatat ttatccctga tatttgtctg
     3661 gttctttttc cttacgaacg gtttctgtga ttaatatatt ctcactgaac accaggaatt
     3721 ctcccaaaac ctgcggtacc gcccgttttc ccgctgtgat agctaccctt aaagactgac
     3781 tcttttttga actgtctctg gaggttgcat gaaagcattg acttatcacg gcccacatca
     3841 cgttcaggta gaaaatgttc ccgatccggg cattgaacag gcagatgata ttattctgcg
     3901 tattacggca acggcgatct gtggctctga tctccatctt tatcgaggca aaatacccca
     3961 ggttaaacat ggcgatattt ttggtcatga atttatgggg gaagtcgttg aaaccggaaa
     4021 ggacgtaaaa aatttgcaaa aaggcgaccg ggtggtaatt ccgtttgtca ttgcttgtgg
     4081 cgactgtttt ttctgtcgaa tgcagcaata tgccgcctgc gaaaatacca atgcgggtaa
     4141 aggcgctgcg ctcaataaaa aacagatacc agctcccgcg gcattgtttg gttatagtca
     4201 cctgtatggt ggcgttcctg gtggacaggc ggaatatgtc cgcgtcccta aagggaatgt
     4261 ggggccgttt aaagtaccgc ctttgctttc agatgataaa gcccttttcc tttctgatat
     4321 tctgccaacg gcatggcagg cagcaaaaaa tgcgcagatc caacaaggtt caagcgttgc
     4381 agtctatggt gccggtccag tgggattgtt gacaatcgcc tgtgcacggt tgctcggtgc
     4441 ggaacagatt tttgttgttg atcatcatcc ctaccgcttg agtttcgccg ctgaccgcta
     4501 cggcgcgatc ccgattaatt tcgatgaaga tagcgatccg gcacagtcaa ttattgaaca
     4561 aacggcaggt caccggggcg tggatgcagt aatagacgcc gtcggttttg aagcgaaagg
     4621 cagcaccacg gaaacggtgc tgactaacct gaaactggag ggcagcagcg gtaaagcgtt
     4681 gcgccagtgt attgcggcgg tcaggcgtgg cggcattgtt agcgtaccgg gcgtctacgc
     4741 tggatttatt cacggtttcc tgtttggtga cgcctttgat aaagggttga cgtttaaaat
     4801 gggacagacc cacgttcacg catggctggg agaattatta ccgttaattg agaaaggatt
     4861 actgaaacca gaagaaattg ttacccacta tatgccgttt gaagaggccg cccggggata
     4921 tgagattttc gaaaaacgtg aagaggagtg ccgtaaggtg attctggtac ccggtgcaca
     4981 aagcgcagag gaggcgcaga aggcagtttc aggtctggtg aatgcgatgc cggggggaac
     5041 aatatgatcg tcaggagtgg ttttcgaggt aaaggacagc catgacgata atcgccgcca
     5101 taatcagaaa tcctatcagg atgtaaaatg cttctgccat ggttattccc acaaacgaaa
     5161 cggaataatt ttgcagcaaa agtgaacagt gagaaccagg aaaaattgct gattttgcgt
     5221 aaagaggatg cgagtgcatc ctctgggcaa agcgagttat cgcttgtgct gatgggatta
     5281 aagcaggtag tcgccagcag cttctggctg gtattcgagt tccagcactt caaggtgggt
     5341 tgcaacgccg cccggaagtt cccagtgaat ggaatcgcca acgcgcagcc ccagcagtgc
     5401 ggcacctacc ggagccataa cggaaagctg agtattgcta tcggtcattt ttgccggata
     5461 caccagcgtg cgcacacgca cttcgccatc gctaagattg cggaatttaa cccggctgtt
     5521 cattgtcacc acgtcgtgtg gcatctcttc tggcgaacac atttgggcgc gatccaactc
     5581 tgcgtttaac gcgtcggcga ttggcaaacc agcataggcg ggttgctcca gcagaatatc
     5641 gatgcgttcg gcatccaggt cgttaatgat gatagttggt ctggacattt ttactccatg
     5701 tcgtcggtac tgcgagtgtc gcagataaac atacccaaaa gaaaaccctc accgtcaggc
     5761 ggcgagggtt taactcacat gatgatactg actgttgctc actctttgaa gtgatttgcg
     5821 tcacattcag ggaattcctc aatgcagcgc attatgtata attcttaatc gtcac
//
LOCUS       DAJVOY010000212         5803 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVOY010000212 DAJVOY010000000
VERSION     DAJVOY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.214"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..843
                     /gene="pdeC"
                     /locus_tag="OQL90_003716"
     CDS             <1..843
                     /gene="pdeC"
                     /locus_tag="OQL90_003716"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS1370336.1"
                     /translation="AGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQPII
                     DIKTEKCIGAEALLRWPSEQEQVMNPAEFIPLAEKEGMIEQVTNYVIDNVFRDLGDYL
                     ATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMTPI
                     ILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHII
                     ELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAREL
                     TRGQ"
     gene            complement(846..1169)
                     /gene="soxS"
                     /locus_tag="OQL90_003717"
     CDS             complement(846..1169)
                     /gene="soxS"
                     /locus_tag="OQL90_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1370337.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1255..1719
                     /gene="soxR"
                     /locus_tag="OQL90_003718"
     CDS             1255..1719
                     /gene="soxR"
                     /locus_tag="OQL90_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1370338.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2265..3614
                     /gene="ghxP"
                     /locus_tag="OQL90_003719"
                     /gene_synonym="yjcD"
     CDS             2265..3614
                     /gene="ghxP"
                     /locus_tag="OQL90_003719"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1370339.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3766..5415
                     /locus_tag="OQL90_003720"
     CDS             3766..5415
                     /locus_tag="OQL90_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1370340.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5569..>5803)
                     /locus_tag="OQL90_003721"
     CDS             complement(5569..>5803)
                     /locus_tag="OQL90_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1370341.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1259 a         1372 c         1599 g         1573 t
ORIGIN      
        1 gccggtattt tggggagtct tgttctgcta ttactctggc tacgtattcg acaaaactat
       61 ttgtctccca aacgcaaatt gcaacgcgct ctagaaaaac atcaactttg tctttattac
      121 cagccaataa tcgatatcaa aacagaaaaa tgtatcggcg ctgaagcgtt gttacgttgg
      181 cctagtgagc aggagcaagt aatgaatccg gcagagttta ttccgctggc agaaaaggag
      241 gggatgatag aacaggtaac taattatgtt attgataatg tcttccgcga tctgggcgat
      301 tacctggcaa cacatgcaga tcgctatgtt tctattaacc tgtcggcctc cgattttcat
      361 acgtcacggt tgatagcgcg aatcaatcag aaaacagagc aatacgcggt gcgtccgcag
      421 caaattaaat ttgaagtgac tgagcatgca tttcttgatg tcgaaaaaat gacgccgatt
      481 attctggctt tccgccaggc tggttacgaa gtggcaattg atgattttgg tattggctac
      541 tctaacttgc ataaccttaa atcattgaat gtcgatattt tgaaaatcga taaatcgttt
      601 gttgaaacgc tgaccaccca caaaaccagt catttgattg cggaacacat catcgagctg
      661 gcgcacagcc tggggttaaa aacgatcgct gaaggcgtcg aaactgagga gcaggttaac
      721 tggctgcgca aacgcggcgt gcgctattgc cagggatggt tttttgcgaa ggcgatgccg
      781 ccgcaggtgt ttatgcaatg gatggagcaa ttacccgcgc gggagttaac gcgcgggcaa
      841 taaaactaca ggcggtggcg ataatcgctg ggagtgcgat caaactgccg acggaaaacg
      901 cgggagaagg tctgctgcga gacataaccc aggtccattg cgatatcaaa aatcggacgc
      961 tcggtggtgc gcaactcaac ggcggccagt aacaggcgac gttggcgaat gtaatcgcca
     1021 agcgtctgat gcgtcaccgt gcggaacatt cgttgcaagt accactttga atagcctgat
     1081 ttttttgcga ctacatcaat gttaagcggc tggtcaatat gctcgtcaat ccatgcgata
     1141 agatcctgaa taattttctg atgggacata aatctgcctc ttttcagtgt tcagttcgtt
     1201 aattcatctg ttggggagta taattcctca agttaacttg aggtaaagcg atttatggaa
     1261 aagaaattac cccgcattaa agcgctgcta acccccggcg aagtggcgaa acgcagcggt
     1321 gtggcggtat cggcgctgca tttctatgaa agtaaagggt tgattaccag tatccgtaac
     1381 agcggcaatc agcggcgata tagacgtgat gtgttgcgat atgttgcaat tatcaaaatt
     1441 gctcagcgta ttggcattcc gctggcgacc attggtgaag cgtttggcgt gttgccagaa
     1501 gggcatacgt taagtgcgaa agagtggaaa cagctttcgt cccaatggcg agaagagttg
     1561 gatcggcgca ttcatacctt agtggcgctg cgtgacgaac tggacggatg tattggttgt
     1621 ggctgccttt cgcgcagtga ttgcccgttg cgtaacccgg gcgaccgctt aggagaagaa
     1681 ggtaccggcg cacgcttgct ggaagatgaa caaaactaaa gcgccacaag ggcgctttag
     1741 tttgttttcc ggtctttgtc tttctctcta tcccgctggt acacaggagg gtttcccccg
     1801 acgtcaacac acctcattcg agcacgtggt ggaggttccg gttggtgttg atgctttaat
     1861 tgtatgtcac cgacgtttct tcgccagtgt aaaagtatac tttttaaccg caatattttt
     1921 gtcatctcag acgatttttt atcgcaatcc tgaacggtat acggctcgat aacgctgcaa
     1981 tcttgcgcac cgacgataac gtttgcgcat caattgcctg ttttttcatc gtcaagacaa
     2041 taaaagagaa aaaagcagca aacttcggtt gaaaaagccg ctatgatcgc cggataatcg
     2101 tttgcttttt ttaccacccg ttttgtatgc gcggagctaa acgtttgctt ttttgcgacg
     2161 cagtaaattg tcgcaaacct ggagcaggaa gataacgttt cgctggcagg ggattgtccg
     2221 ccacgcatct tgacgaaaat taaactctca ggggatgttt tcttatgtct acgccatcag
     2281 cgcgtaccgg cggttcactc gacgcctggt ttaaaatttc acaacgtgga agcactgtcc
     2341 gtcaggaagt ggttgccggg ttaacaacgt ttctggcgat ggtctactcg gtcatcgtcg
     2401 ttccaggtat gttgggtaaa gcaggcttcc cgcctgcggc agttttcgtt gcaacctgtc
     2461 tggttgccgg actcggttct atcgtgatgg gtctgtgggc taatctgccg ttggcgattg
     2521 gttgcgccat ctccctgacg gcgtttaccg cattcagcct ggtgctgggg caacatatta
     2581 gcgtacctgt cgcgctgggt gccgtgttcc tgatgggtgt gctgtttacg gtaatttctg
     2641 ccacgggtat ccgtagctgg attttgcgca acttgccgca cggtgtggcg cacggcacgg
     2701 ggatcggtat cggtctgttc ctgctgctca ttgccgctaa tggtgttggt ctggtgatta
     2761 aaaacccgct tgatggtctg cccgttgcgc tgggtgattt cgcgaccttc ccggtgatta
     2821 tgtcactggt aggtctggcg gtgatcatcg gcctggaaaa actgaaagtc cctggtggca
     2881 ttctgctgac cattatcggt atctcaattg tcggtttgat cttcgatcct aacgtccatt
     2941 tctccggcgt tttcggcatg ccttcattga gcgatgaaaa cggcaattca ctgattggca
     3001 gcctggacat tatgggcgcg ctgaatcctg tagtcctgcc aagcgttctg gcgctggtga
     3061 tgacggcagt atttgatgcc accggaacta tccgtgccgt cgccggccag gcgaacctgc
     3121 tggataaaga tgggcagatc atcgacggtg ggaaagcact gaccactgac tccatgagca
     3181 gcgttttctc tggcctggtg ggtgctgctc cggcagcggt atacatcgag tctgcggcgg
     3241 gtacggcggc gggcggtaaa accggtttga cggctatcac cgttggcgtg ctgttcctgc
     3301 tgattctgtt cctctctccg ctctcttacc tcgttccggg gtatgcaacg gctccggcgc
     3361 tgatgtacgt tggcctgctg atgctgagca acgtggcgaa aatcgacttt gctgattttg
     3421 ttgatgcgat ggcgggtctg gttacggcgg tattcatcgt gctgacctgt aacatcgtaa
     3481 caggcatcat gatcggcttc gcgactctgg tgattggtcg tctggtttcc ggcgaatggc
     3541 gcaagttgaa catcggtacg gtcgttatcg ccgtggcgct ggtgaccttc tatgcgggtg
     3601 gctgggctat ctaatctttc atcggattct gaaaacgggt ggcaatggct gcccgttttt
     3661 attttctccg cacattgtgt gagcttttgc gatattctga aaaaatgaga attcaggcat
     3721 aacgcctttc tcagatcata aaaaaacatc gcaaacaggg aacgcatgga aatcttcttc
     3781 accatactga taatgaccct cgtggtctcg ctgtccgggg tggtcactcg tgtcatgccc
     3841 tttcagatcc cgcttccgct tatgcaaatc gccatcggtg cgctactggc gtggccgacg
     3901 tttggtttgc atgtggagtt tgatcctgaa ctctttttag tcttgtttat cccgccgttg
     3961 ctgttcgctg atggctggaa aacgccgacc cgtgaatttc tcgaacatgg tcgagagatt
     4021 ttcggcctcg cgctggcgct ggtggtggtc accgtggtcg gcattggctt ccttatttac
     4081 tgggtggtgc cgggcattcc gctgatcccc gcctttgcgc tggcggcggt actttctccg
     4141 accgatgctg tggcgctctc cggaattgtt ggcgaagggc gcatcccgaa aaaaatcatg
     4201 ggcattttgc agggcgaagc gttgatgaat gacgcctccg gcctggtgtc gttgaagttt
     4261 gccgtggcag tggcgatggg gacgatgatc ttcaccgtcg gcggtgcgac ggtcgaattt
     4321 atgaaagtag ccattggtgg tattcttgcc ggttttgtgg tgagctggct gtatggtcgc
     4381 tcgctgcgat tcctcagccg ctggggcggt gatgaacccg cgacgcagat cgttctgctg
     4441 ttcttgctgc cattcgcttc ttatctgatt gccgaacata ttggcgtttc cggcatcctc
     4501 gctgcggttg ccgccgggat gaccatcacc cgctccggtg tgatgcgccg tgcgccgctg
     4561 gcaatgcgcc tgcgcgcaaa cagcacctgg gcgatgctgg aatttgtctt taacggcatg
     4621 gtgttcctgc tgttaggtct gcagctgccg ggtattctgg agacgtcgct gatggcggca
     4681 gaaatcgacc ctaacgtcga aatctggatg ctgtttaccg atattattct gatatatgcg
     4741 gcgctgatgc tggtccgttt cggctggctg tggacgatga aaaagttcag caaccgcttc
     4801 ctgaagaaga agccgatgga gtttggttcg tggaccacac gagaaatcct gatcgcgtct
     4861 ttcgccgggg tgcgtggggc gatcactctg gccggtgtgc tctctatccc gctgctcttg
     4921 ccggatggta acgtcttccc ggcgcgctat gagctggtgt tcctggcggc tggcgtcatt
     4981 ctcttctcgc tgtttgtcgg tgtggtgatg ttgcctattc tgctacaaca cattgaagtc
     5041 gcggatcatt cgcaacaatt gaaagaggaa cgtattgcgc gagcggcaac ggcagaagtg
     5101 gcgattgtgg cgatccagaa aatggaggag cgtctggcgg cggataccga agagaatatc
     5161 gataaccagc tgcttacgga ggtcagttct cgcgtcattg gtaacctgcg tcgtcgcgcc
     5221 gatggacgta acgacgttga aagttccgtg caggaagaga accttgagcg tcgcttccgt
     5281 ctggcggcat tgcgttctga acgtgctgaa ctgtaccacc tgcgcgccac gcgggagatc
     5341 agcaacgaaa cgctgcaaaa attactgcac gatctcgatt tgcttgaagc gttgctaatt
     5401 gaggaaaatc agtaagccga aagcgacgta accaggcccg aggtaatctg aaaaaggtgc
     5461 aatcttttgc acctttaaca ggttagctcg ggctaacatt catgattcta aaacaaataa
     5521 gttatctctc ttgtaaagtc aattcattgg ctgttcttcg acttaacctc aatctgcgtg
     5581 ttgcataata tctgcagtaa tcttcatagc gactgcatta atattatcct cagtttgtga
     5641 catgccagta tgagaggtac aatcttcttc aaagaatttc tgtagtgtta ccagggtatc
     5701 actatcaatg gggatatttt cctctccacg gagtatggaa atgcccattc cctggacgct
     5761 agcctcatca aaattaaccc gatagagacg ggtattaaac ata
//
LOCUS       DAJVOY010000213         5750 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVOY010000213 DAJVOY010000000
VERSION     DAJVOY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.215"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..383)
                     /locus_tag="OQL90_003722"
     CDS             complement(<1..383)
                     /locus_tag="OQL90_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="regulates several genes involved in high affinity
                     phosphate uptake; under conditions of high phosphate
                     concentrations downregulates the PHO regulon; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate transport system regulator PhoU"
                     /protein_id="HCS1370342.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSL"
     gene            complement(398..1171)
                     /gene="pstB"
                     /locus_tag="OQL90_003723"
     CDS             complement(398..1171)
                     /gene="pstB"
                     /locus_tag="OQL90_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1370343.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1262..2152)
                     /gene="pstA"
                     /locus_tag="OQL90_003724"
     CDS             complement(1262..2152)
                     /gene="pstA"
                     /locus_tag="OQL90_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1370344.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2152..3111)
                     /gene="pstC"
                     /locus_tag="OQL90_003725"
     CDS             complement(2152..3111)
                     /gene="pstC"
                     /locus_tag="OQL90_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1370345.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3198..4238)
                     /gene="pstS"
                     /locus_tag="OQL90_003726"
     CDS             complement(3198..4238)
                     /gene="pstS"
                     /locus_tag="OQL90_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1370346.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4486..5559)
                     /locus_tag="OQL90_003727"
     CDS             complement(4486..5559)
                     /locus_tag="OQL90_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1370347.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(5570..>5750)
                     /locus_tag="OQL90_003728"
     CDS             complement(5570..>5750)
                     /locus_tag="OQL90_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370348.1"
                     /translation="LADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLST
                     PESESGLIEQGVTCH"
BASE COUNT         1467 a         1490 c         1367 g         1426 t
ORIGIN      
        1 agacttacca gcaacggctg atgctgctgg gagaatttct ccagcgcagt acggcagatt
       61 ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg ttttactgat caccataacc
      121 agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg
      181 atcgccactt ccatcatgtt gacgttcttg tcgccttcga tcacgcgctt cgccagatcg
      241 ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct gctgctccac catgccgccc
      301 atggtcatca cctgcgtgcg gatactttcc agttcggcgt tgaactggcc ggaaatatgt
      361 ttattaagat tgagactgtc cataacgcac tcctgaatca accgtaacga ccggtgatgt
      421 agtcttctgt ttgtttcttc gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa
      481 tcaattcgcc caggtacata aacgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt
      541 tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac
      601 gcccggtaga gatagggtcg agcgccgaac acggttcgtc gagcagcagc acttccgggc
      661 gaatggcgat accacgcgca atacacagac gctgttgctg accaccagag agagagtaac
      721 cgctctggtg caatttatct ttggtttcgt tccacaatgc ggctttggtc aatgcccact
      781 gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa cagacgaacg ccaaaagcga
      841 tgttgtcgta gatggacatc ggaaacggcg tcggtttctg gaacaccatg cccactttcg
      901 cacgcagcag tgcgatatcc tgagagttgg tcaggatgtt gtcgccatca agcagaattt
      961 caccttccgc acgctgctcc gggtacagtt caaacatttt gttgaaggta cgcagcagcg
     1021 tcgatttacc gcagccggac ggcccgataa acgccgtaac ctggttttta gcgatatcca
     1081 ggttgatgtt tttcagggca tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa
     1141 ttttactcgg ggcagtttca accatactca ttgcaatctt ttcctcatcg tgcctgatgc
     1201 acttcgctaa tcaggcttac cgtaggccgg ataaggcgtt cacgccgcat ccggcaaagt
     1261 gttaaccgtg tttattcttc gcaaaaacaa cgcgcgctag aatgttcagc agcagtacgc
     1321 acagggtaat aatcaatacc ccggcccagg ccagttgttg ccattccgca aacgggctca
     1381 tcgcaaactt aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc
     1441 agaactggtt ggagagcgcg gtaaacagca gcggcgcggt ttcaccagca atacgggcaa
     1501 tcgccagcag gataccggtc atgatcccgg acaccgacgc tttcagcgta atcgcagaga
     1561 tcatcttcca cttcggtgta cccagcgcat aagccgcttc acgcaggctg tccggcacca
     1621 gtttcagcat gttctcggtg gtgcggataa caatcggcac ctgcaacagc gccagggcaa
     1681 tcacgcccgc ccagccagag aagtgctcca tctgcgccac cacgatggtg taaacaaaca
     1741 gaccaaccac aatcgacggc gcggagagca gaatgtcgtt aatgaagcga atcacttctg
     1801 ccagccagga tttacgacca tattccgcca gataaatccc cgccataatg cccagcggcg
     1861 taccgaatac cgtggcccac aaaattaaca gcccgctacc cgccagggcg ttcgccagac
     1921 caccaccttc cgtattgggc ggcggcgtca tttcagtgaa cagcgccagc gacataccgt
     1981 cgataccgcg agtgatggtg gacattaaaa tccagatcag ccagaacagg ccgaaggcca
     2041 tcgtcgccat cgagagcgtc agcgcaatac ggtttttgag gcggcgacgc gcctgcattt
     2101 tgcggcgaga ttcagccagc gccgcagtgg tttggatttc aaccatagcc attagcgtgc
     2161 cccctcattc ttagccaggc gcataatcat aaacttcgat gcggcgagga cgatgaaggt
     2221 aatcacaaac aggatcaggc ccagttccat cagtgcggca acgtgcagac cggattccgc
     2281 ttccgcaaat tcgttcgcca acgcagaggt gatactattg cccggcatgt acagcgaggc
     2341 gctgtcgagc tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccgagcgc
     2401 gcggcccagc cccagcatga tgccgccgat aacaccattt ttggtgaacg gaagaacgat
     2461 acgccagata acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac
     2521 cggggtttgt tcgaacacat cacgcattac cgccgcaatg tacggaataa tcatgatggc
     2581 gaggatcacg cctgccgcga ggataccgat accaaatgcg gggccagaga acagcgcgcc
     2641 aacaatcggg atattcgaca tgatattgcc gaccggctcc tgaaagtaaa cggcgaacag
     2701 cggcgcaaag ataaacaggc cccacatgcc gtaaacgata cttggaatgg ctgccagcag
     2761 ctcaatggcg atacccagcg ggcgtttcag ccagccaggc gcaagctcag tcaggaacag
     2821 ggcgataccg aaactcaccg ggacggcgat cagcagcgcg ataaacgaag tcaccaacgt
     2881 accgtagatc ggcaccagcg ccccgtagat atcgttcggt gcatcccact ctttggtcca
     2941 taggaaagcc agaccaaatt tctgaatgct cggccaggag gagatgatca gagagacaat
     3001 aatgccaccc aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat
     3061 tatgtcgccc tttttacccg gtgggttaaa agcaggcttg gttgcagcca taagttactc
     3121 ttcagttaaa cgcgtttaca aagtcatttt gtaatgccgg atgcggcgta aaacaccgta
     3181 cccggcctgg agttttatta gtacagcggc ttaccgctac tgtctttaat attggtcttc
     3241 cacgcagcgc gaacctgttc aactacacta tccggcaggc tggcgtaatc caggtcgttc
     3301 gcctgtttag ccccggtttt gtacgcccag tcgaagaatt ttagcacttc tgtgccttgt
     3361 tctggtttct tctgatcttt gtggatcaga atgaacgtgg tagaggtaat aggccatgca
     3421 tcttcgcctt tctggttggt cagatcctga gcgaaggttt tgctccagtc tgcacctttt
     3481 gctgcattag cgaagttttc ttcggtcgga ctaaccggtt taccatcagc ggagatcagt
     3541 ttggtgtacg ccaggttgtt ctgcttcgcg taagcatatt caacataacc aattgcaccc
     3601 ggcagacgct gaacgaacgc ggcgataccg tcgttacctt taccgcccag accgatcggc
     3661 cattttacgg tagagccagt accaacgttg tttttccact cttcgttcac tttcgccagg
     3721 tagctggtga agacgaagga agtcccggag ccatctgcgc ggcgtactac agcaatgttt
     3781 tgtgaaggca gtttcagacc cggattcagt ttggcgatgg cttcatcatc ccacttcttg
     3841 attttgccca ggtagatgtc gccgagggtt ttaccatcca gcaccagttc gccagacttc
     3901 agccctggaa tgttaaccgc cagcaccacg ccgccaatca cggtcgggaa ctggaacaga
     3961 ccttcctgag ccagtttttc gtcagacagc ggcgcgtcag aggcaccaaa atcaacggta
     4021 ttagcgataa tctgttttac gccaccggaa gaaccgatac cctggtagtt aactttatta
     4081 ccggtttctt tctggtaagt gtcagcccat ttggcataca ccggcgcagg gaaggttgca
     4141 cctgcacctg tcaggcttgc ttctgcaaac acagagaaag cgctcatcga taaggtcgcg
     4201 gcgacaacag ttgcgacggt ggtacgcata actttcataa tgtctcctgg gaggattcat
     4261 aaagcattgt ttgttggcta cgagaagcaa aataggacaa acaggtgaca gttatatgta
     4321 aggaatatga catttttatg acagaaagga attaccaaat aacttactga ttttaaatgt
     4381 gaagatgaaa atcccacatg tcatcttttt gtcatgatgc atttaaaagt gataaagaaa
     4441 tgggtgagat gacaacgatg tgatgaacaa tcgttgtcag acctattagc gcacaatctc
     4501 aacagttatc gtcgctgttg cagtaaattg ccccggggct gggcgagctc cagtaaggct
     4561 cgcaggagca gctgaaaaag ttacactacc tgatttttca ccatacactg ttgatttacc
     4621 catgtctgtt ggcaactcac caccgttcac tttgatttcc ttattatttt tgtcgtaaat
     4681 ttttatcccc acgttagagt ttgaagttac gaccattgaa ttgtccgtat tgggttcggt
     4741 agcataaaaa gacgtactga tatcatcggt attcagtatc cccaggtttt cacactcaat
     4801 agtgaccgtt ttagtaattt tatgtgttat taccgcactt ccaacaacag atgaaaaatc
     4861 tgcagctggt atttctttaa aatcaaaata aactggctgt aaattattta tttcgcaact
     4921 ttgcggagct gtaatatcgc cccgaatata aacatcagcc atcggctgtg agtaatccac
     4981 cggggtgtca cggctaattg ttccatacaa tgtcactatc tttgtttcag gaataatcaa
     5041 ttttcctgca aatgtcttct tgatataaaa atatactatc gctttagccc cactagatat
     5101 acttaaaggt tcaatgcggt tgcaattgta ctttgtatta ccagacgggt tgttagcatg
     5161 atattcaaaa ggtgcattca catacccaac cccaagaata tctatagcaa tcccaacatc
     5221 aaggttcgga ttaagatcat aataattaat attagagtga accccagcat aagttaatgc
     5281 ctggtttatt tttgacgtat aatagatata ggggaattct cctacgttac cagtatcaca
     5341 atgacagcgt ccaggatatg aggatccatt agttacagtg gtgttaaatg tcgttccaac
     5401 cttattttcg ttcgcggtaa taagatatcc cgagaaatca agcgtactgt gaaatacgcc
     5461 attatcaggg gtacagatac ctttaggagc tgcaagtgct acactactta acgagagcat
     5521 aaacacagca aagcaccact gtttgatata cttgttcata aaaatccttt taatgacatg
     5581 tcacaccctg ttcaattaat ccgctctcac tttctggtgt cgaaagtgaa taattgaaga
     5641 tgcattttgt tgaggcgttt tctccccagg ataacgtaac ctgtcctttt tctggtagac
     5701 cagaaagata aagttcgcca cggtcaccga caattcctga atctgccaaa
//
LOCUS       DAJVOY010000214         5745 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVOY010000214 DAJVOY010000000
VERSION     DAJVOY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.216"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..383)
                     /locus_tag="OQL90_003729"
                     /pseudo
     CDS             complement(1..383)
                     /locus_tag="OQL90_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(569..3877)
                     /gene="icsA"
                     /locus_tag="OQL90_003730"
                     /gene_synonym="virG"
     CDS             complement(569..3877)
                     /gene="icsA"
                     /locus_tag="OQL90_003730"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCS1370349.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4406..5608
                     /gene="virA"
                     /locus_tag="OQL90_003731"
     CDS             4406..5608
                     /gene="virA"
                     /locus_tag="OQL90_003731"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCS1370350.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1777 a         1299 c          949 g         1720 t
ORIGIN      
        1 cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt aggatcttca
       61 ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct gttactaatt
      121 tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc tacttggaaa
      181 tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg tacagaacta
      241 ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat tgctcgacct
      301 tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg aggcgcataa
      361 ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc tgtgtaacgc
      421 caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg atcgcaggca
      481 taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag agaaatgcag
      541 gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc aaaatacctt
      601 gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag atgcttaggt
      661 tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg ttgcctatct
      721 ggtgatttga catgccattc ataattacac catattgctt tgagctccat ttccaattca
      781 cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaacta tgactaatta
      841 aggatgtacg caatcccaat tttgcttgaa tattattgac cccagaggat gtgaccatag
      901 tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc tggacttgtg
      961 gattgagata caatgcgttg tcaatattat tatgagtagt ccagcgtatg gttgggtaga
     1021 tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca ccagttaacc
     1081 catctccttt gactgtatta ttaaaccaac cgtactgcaa ccaagcatcc atatatagac
     1141 ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta taaccgctca
     1201 cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc tgatatccat
     1261 aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc ttcaaaatat
     1321 ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta tcagctaaat
     1381 tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg agctgacctg
     1441 tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt
     1501 tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg tgaatactac
     1561 cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta ctcatttgag
     1621 tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta gttagatacc
     1681 acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca taactaccag
     1741 caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc gaaataattt
     1801 gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca acataggtaa
     1861 taccagacgc atggcctgta atttccagcc ggtcagtttt actgttgtca ttgccgagaa
     1921 ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga acataggtct
     1981 gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc agagtcatgt
     2041 tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg attagcacag
     2101 tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac
     2161 ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc atttttagaa
     2221 tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa atgagagttc
     2281 cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga tggctgagat
     2341 tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg aactctccat
     2401 gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc tggtcactga
     2461 tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg atagatatat
     2521 tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag tccttctgcg
     2581 cccagttatt tataatagct tccactttta cattgttgcc aaatgacata tttccagaag
     2641 cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac
     2701 ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca gcacataaat
     2761 caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc agaatggatg
     2821 aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt aatgaattgt
     2881 ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca tcttctttac
     2941 ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata gcatcccccc
     3001 aatgtccacc attaccgcct aaaatagctc cattgttgat tatagacagg ttatctcctg
     3061 tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct ccgttgcctc
     3121 cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca tcacctccat
     3181 taccgccatc accctcacca taattgttac ctccattacc accggaaata gtccctttgt
     3241 tgattatggg tagattgact cctgtgacgg catcacctcc attcccccca tcactatcac
     3301 tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata aacagattgt
     3361 ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg ccgctaccac
     3421 caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag tcaccaccat
     3481 taccgccaca actattacca tcattattat caccagcacc accatgacct gcgctaatca
     3541 taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg gaagaagtta
     3601 tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg
     3661 ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc gaaagaggaa
     3721 tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag
     3781 acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc tgtgaacatt
     3841 gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc agtaagtggt
     3901 tgataaaccc ctaaaaagat tccgttttat ccggaataaa gggacgatat atgcaaaaac
     3961 atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat gcaacgggag
     4021 aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt cgataataaa
     4081 ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct ttctgtacgc
     4141 ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc tgccccttga
     4201 tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc taccagactc
     4261 tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct taaagagaag
     4321 aataacattc cattttatta tgttcccaat ttaattatat taatatatat tggcatcaat
     4381 aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc atgaaagaaa
     4441 tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga ataaattgag
     4501 tttttgtgac gttttactga aaataataac tttcggaata tattctccac acgaaactct
     4561 agccgaaaaa tatagcgaaa aaaaactaat ggatagtttc tcaccatcac tatcacaaga
     4621 taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac ggctatgcct
     4681 taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat ccactcaact
     4741 ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat tcaacttagg
     4801 aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca agcctgcacc
     4861 agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg atgattactt
     4921 tcccgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag atattcgagc
     4981 atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac taaaattaat
     5041 tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa aagagcttca
     5101 aatgctaagt tacaaccaat taaataaaat aatcaattca aacttccccc aacaagacct
     5161 atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg catttatatc
     5221 cgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg tggaacacat
     5281 atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc ctgaacaacg
     5341 agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta aaaacgacac
     5401 tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat taccatcgca
     5461 cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg ttcatgctgg
     5521 aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc
     5581 atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca gactgtaaaa
     5641 aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga tggggaccaa
     5701 tagaatctca gcccatcctt tttatatcat tatgaaccgc cccgg
//
LOCUS       DAJVOY010000215         5721 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVOY010000215 DAJVOY010000000
VERSION     DAJVOY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.217"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..257)
                     /locus_tag="OQL90_003732"
     CDS             complement(<1..257)
                     /locus_tag="OQL90_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370351.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYR"
     gene            510..827
                     /locus_tag="OQL90_003733"
     CDS             510..827
                     /locus_tag="OQL90_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCS1370352.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            896..1225
                     /gene="yjdP"
                     /locus_tag="OQL90_003734"
     CDS             896..1225
                     /gene="yjdP"
                     /locus_tag="OQL90_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCS1370353.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1372..2130)
                     /gene="phnP"
                     /locus_tag="OQL90_003735"
     CDS             complement(1372..2130)
                     /gene="phnP"
                     /locus_tag="OQL90_003735"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCS1370354.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(2132..2581)
                     /gene="phnO"
                     /locus_tag="OQL90_003736"
     CDS             complement(2132..2581)
                     /gene="phnO"
                     /locus_tag="OQL90_003736"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCS1370355.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVSSKL
                     LAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2568..3125)
                     /gene="phnN"
                     /locus_tag="OQL90_003737"
     CDS             complement(2568..3125)
                     /gene="phnN"
                     /locus_tag="OQL90_003737"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCS1370356.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(3125..4261)
                     /gene="phnM"
                     /locus_tag="OQL90_003738"
     CDS             complement(3125..4261)
                     /gene="phnM"
                     /locus_tag="OQL90_003738"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCS1370357.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(4258..4938)
                     /gene="phnL"
                     /locus_tag="OQL90_003739"
     CDS             complement(4258..4938)
                     /gene="phnL"
                     /locus_tag="OQL90_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCS1370358.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(5049..>5721)
                     /gene="phnK"
                     /locus_tag="OQL90_003740"
     CDS             complement(5049..>5721)
                     /gene="phnK"
                     /locus_tag="OQL90_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCS1370359.1"
                     /translation="PGEVLGIVGESGSGKTTLLKSISARLTPQQGEIHYESRSLYAMS
                     EADRRRLLRTEWGVVHQHPLDGLRRQVSAGGNIGERLMATGARHYGDIRATAQKWLEE
                     VEIPANRIDDLPTTFSGGMQQRLQIARNLVTHPKLVFMDEPTGGLDVSVQARLLDLLR
                     GLVVELNLAVVIVTHDLGVARLLADRLLVMKQGQVVESGLTDRVLDDPHHPYTQLLVS
                     SVLQN"
BASE COUNT         1165 a         1614 c         1684 g         1258 t
ORIGIN      
        1 cgataatatg gtttgccatt agcatcgcgc agaatacctt catccagcag cggagcgata
       61 aatccacgct gagcgtcgtc aaaatcctga cgatcgctaa acggtaatga tttggcgtaa
      121 ttctgattaa cctgttgggt atattgagtg gcggctttcg cttcctcttt cgcgttagcg
      181 gtatttacca tcatgccgct ggcggcagtt aacgcaataa caattctgct caaacggaat
      241 aaccgagagt tattcatata aatctccatt tatcatttat gtcgtaaata tgtaatccat
      301 acaaaatacg tcatttaaag ctggggttat cataattggg aatttgcgac tttaataagt
      361 ggaagtgtga gctgaacgat ccattttatt aggcatttgt gctgaagaga gaaaattacg
      421 cgaatttatt atctctgtaa ataatatgag gtgttttata tatttattat atatattgta
      481 cttcggcaaa tactgagcac aatttatcaa tgcaaccggt gtttcttttt aaaaaacgta
      541 ccggagcaat gggcggtcat gtcgctggca ccgcaggatt ctgcgttgct gtgccagcct
      601 tttattcatg gtcaatgagt gaggtgattg ataaagacac caggtcgtca gagcgcactg
      661 attatccgca ttatgccagt gaagttgcac tggcttttgg ttcacgagtg gttggcctcg
      721 aactggcaaa aatgattgtg gatgcgtggc tgggcgcaca gtacgaaggc ggtcgtcatc
      781 aacaacgcgt ggaggcgatt acggcaatag agcagcggag aaattgagat tcatccacta
      841 cttgcatgga tgagtaatga ttaatgtgga tagagtttct ttttgaggtt ggctaatgaa
      901 acgctttccg ctatttcttt tattcaccct gctcacgctg tccaccgttc cggcccaggc
      961 cgatattatc gacgacacca ttggcaatat tcagcaggcg attaacgacg cttataaccc
     1021 tgaccgtggg cgagactatg aagattcgcg cgacgacggc tggcagcgtg aagtgagcga
     1081 cgatcggcgc agacaatatg acgaccgccg ccgccagttt gaagatcgcc gcaggcagct
     1141 ggacgatcgc cagcgccagc ttgatcagga gcgtcggcag ctagaagatg aagagcggag
     1201 aatggaagat gagtatgggc gatgaggttg ggtatggagc aggcatcgcc cctcacccca
     1261 atggggagag ggagaaaacc agcgcaatat tcaatatcga gcacaatcgg tcccctcgcc
     1321 cctctgggga gagggttagg gtgagggggc cagcgcgcac attccctctc atcacgccac
     1381 cccaatctcc atcccatcaa atcccgcctc aaaccccgac ggcagtgcgt tttccatcag
     1441 ccacgcatca aactggtggc tgatatgggt cagaatcacc cgtggcgagc ggataacctg
     1501 attcagcgcc agcacggtgt ttaaatcaca gtgactacgt ggcgcatcct cgcgcggcgg
     1561 gtggctgcaa tcaatcacca ttacctgcgg ctgattattg cgtaaaaatt tcagcgtttt
     1621 ttccggcaag cctgcggtgt cagacagcca cgccacccgg ctgtgtgccg tttccagcag
     1681 ataaccgaag gtcagttttg agtggttgag cggcaggggc gtgacctgta acccctgcaa
     1741 atcaaacacc acaaacggtt ccaccgtgtg gctgaaatca agcaggcccg gatgtttaaa
     1801 cagatcgtcg cagccctgtt catccggcgg gccgtaaacc gggatcggat cgcccacgcc
     1861 ccagcgcagc gggaatagcc cctgcacgtg atccatatgg tagtgcgtca gcaaaaactg
     1921 ctggaacgat ccgggcggcc agcgatcggc gagatcgtgc agcccggcgt cgatcagggt
     1981 gattgcgtcg ttaaacttca ctacgccgct gcatggctgg cggcgatact gcggtgagcg
     2041 ccgcgctctg gcgcaggcta cgcactcgca gccccatgcc ggaacgccct gtgcgccgcc
     2101 ggtgccggtg agcgtgaggg tcaggctcat gttacagcgc cttggtgaag cggaagtggc
     2161 tctgctcgta gccttcacgc aggtaaaaac ggtgcgcgtc gtggcgcttc acgttggtgg
     2221 aaagttcggt catttcggcc ccggcctggc gggcttcttc ttctgcccac gccagtaact
     2281 tactgctgac gttcagaccg cacgcctgcg gcatcaccac cagttcctga atttcgccga
     2341 tccagttgac atgatgcaga tgaaactgca aatgcaggcc gatcatgccg acaacttcgc
     2401 catccagcag cgccagatgg tagcgcatgc tcgggtagcg catgctcggg tcgcgcaggt
     2461 tggcgttaaa gcccacgcga aacgcgtggt ggtcaaactc cgcctgtttt agctcacaaa
     2521 tcagcgcgta aaccgcgtcg gtatcgtact gcgtggccgg gcgaagctca caagcaggca
     2581 tggtgtttct ccttctgatg aatcagcgtc agcagtgtgt cgaccgactg gcgcaggctg
     2641 ccgtcattgt tgagcgtatg gcaatcctgt ggagtatagc gggcggcgcg cgccagccgg
     2701 gcgttaattt cactggtgtt ttcacggcca cggtttttca ggcgctggcg caggatctcc
     2761 ggcgaaacct gtaaacagac gggcagcagc gccgattgat agcgcgcccg tgcctgcggc
     2821 aaatgcgcgc gtgaaccgtt gacgactacg tcgaatccag cgtgcagcca gaggtcaatc
     2881 tcgatgccga cgccataata cagaccgtta gcgtgccagc ttaaggccag caggttttgc
     2941 cccgcgcggg taaaaaactc ccgctcactc agggcgatat ggttttcact tccggcgctg
     3001 gcgtcgcgcg tgatgtagcg atgcgccacc agtaactgcg tttgttcccg caggcggagt
     3061 tccgccagca ggctgtcttt cccggagccg gacggcccca ttaaccaaat cagttttccc
     3121 atcatcagaa caccctttta ccctgacgcc agacgtggtc gatatgaatg tgattgccct
     3181 ggcgatgcgc cagcaccagg tcagcgcgtt taccctcgcc aatcaccccg cgatcctgaa
     3241 gattcagcgc ctgcgccgga tttttggtca ccagcctcac cgcctgcggt agcgtaaagc
     3301 ggttgctctc gtcatcggcg acgcggaacg ccgcatccag caggctggcg gggtagtagt
     3361 cggaagagag gatatccagc agaccaagct gcgccagttc actggccgcc acattgccgg
     3421 agtgcgagcc gccacgcacg atatttggcg cacccatcag cacgttcatg ccatgcttac
     3481 gcgaggcttc cgccgcttcg aacgtggtgg taaattcggc gatcacgctg ccaagttggt
     3541 gggattcggc aacgtgggcg tgcgtggcgt catcgtggct ggcgagcgca atttgtcgcg
     3601 cgcggcacag ggcggcgatg gattcgcgat tcggctgcga ccagcgtgcg gcgagcgcca
     3661 attgctcttc ttcgtactgc tgcatctgcg catcggtgag ggagtatttg ccctgataat
     3721 attcgcgata cttctcgcgg ttagcgaact ggcgctggcc cggtgagtgg tccattagcg
     3781 acaccagcgt caccggttcg cgctgaacca gtttttcaaa cagcggcagt gtggtgtgat
     3841 gcggcagctc gcagcgcagg tgcagacggt gctcggcgcg gttgacgccg cgtttctgcg
     3901 tctcttcgat ggtgttgatc atcttctcca gattctccag ctgatcgccg ccgtcgcgca
     3961 cgtcgccaat cgccaccgcg ttcagcacgg tggtgatgcc gcttgccacc atcagcgcgt
     4021 cgtggctgct catcgccgaa tgggcgggcc agtcgacttt cgggcgcggg gtgaagaatt
     4081 tatcgaggtt gtcggtatgc agctcaatca ggccgggtag tagccagcca ccttcgccgt
     4141 ccatcgcttc tggcaggcgg ctctggcttt cggcaaaggc gcggatttcg ccatcctgca
     4201 cctccagcga accgtgcacc acctcgtttt ccagcaccag cttaacgtta ttgataatca
     4261 tgaagaggct cccattgggt gcaggcggtc ggcgacgtca ttacgtacag cttcgtcatg
     4321 gaagatgccg acgatggctg cgccacgggc tttggcttcg tgaatcagtt ccaccaccgc
     4381 ggcgctgttt ttggcgtcca gcgaggcggt aggttcgtcg agcagcagaa tggggtagtc
     4441 gacgataaag ccgcgggcga tgttgacgcg ctgctgttcg ccgccggaga aggtcgatgg
     4501 tgccaggtgc cacaggcgtt ccggcacgtt caggcgggtg agaagacgcg cggctttagc
     4561 ggcgcaggct tcacgcggaa cgccggtatc gagcagcggc tgcatcacca cttccagtgc
     4621 tgagatacgc gggatgacgc gcagaaactg gctcacccag ccgactgtgg ttttgcggat
     4681 ttccaccact ttgcgcgctg gcgcggtgac caggtctacc cactcgtcac cgtgtttgat
     4741 ctggatttga ccttcgtcgg gtagatagtt ggcgtacagc gagcgcagca gggttgattt
     4801 gccgctgccg gagtgaccgt ggagcaccac gcattcgcct gcgttgacgg tgagcgaggc
     4861 acgttgtaag acgggcaggc gtacgccgtt ttgctgatgc aggatgaagg ttttactgac
     4921 gttttgtacg ttaatcattt ttcgcctcgt ggttcgggtg tcggatgcga cgctgacgcg
     4981 tcttatccga cctacgggga gcgcatttgt aggccggata aggcgtttac gccgcatccg
     5041 gcaccggctc aattctgcaa aaccgatgac accagcagtt gtgtatacgg atggtgcggg
     5101 tcgtcgagca cgcggtcggt taacccactc tccaccactt gcccctgctt catcaccagc
     5161 aaacggtccg ccagcaggcg ggcgacgcct aaatcgtggg tgacaatcac taccgcgagg
     5221 ttcagctcca ccaccaggcc gcgcagcagg tcgagcaggc gtgcctgtac cgacacatcc
     5281 agtccgccgg tcggctcgtc cataaacacc agcttcggat gcgtcaccag gttgcgggcg
     5341 atctgcaaac gttgctgcat gccgccggaa aaggtggtcg gcaggtcgtc gatccggttg
     5401 gcgggaattt ccacctcttc cagccacttc tgcgcggtgg cacgaatatc gccgtaatga
     5461 cgtgccccgg tcgccatcag ccgctcgccg atattgccgc ctgccgacac ctggcggcgc
     5521 aggccgtcga gcggatgctg atgcactacg ccccattcgg tgcgcagcag gcgacggcgg
     5581 tcggcctcgc tcattgcgta cagcgaacgg ctctcatagt gaatctcccc ctgctgcggc
     5641 gtcaggcgcg cggagattga tttcagcagc gtggtctttc cggagccgga ctccccgaca
     5701 atgcccagca cttcccccgg c
//
LOCUS       DAJVOY010000216         5660 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVOY010000216 DAJVOY010000000
VERSION     DAJVOY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.218"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            19..1404
                     /locus_tag="OQL90_003741"
     CDS             19..1404
                     /locus_tag="OQL90_003741"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1370360.1"
                     /translation="MEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEA
                     IDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
                     GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTCYKEKVKYWMTFNEIN
                     NQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFN
                     IGCMIAMYPIYPLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLD
                     ITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQ
                     IDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKK
                     AVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWY
                     RDLIANNGENI"
     gene            complement(1461..1679)
                     /gene="marB"
                     /locus_tag="OQL90_003742"
     CDS             complement(1461..1679)
                     /gene="marB"
                     /locus_tag="OQL90_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1370361.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1711..2094)
                     /gene="marA"
                     /locus_tag="OQL90_003743"
     CDS             complement(1711..2094)
                     /gene="marA"
                     /locus_tag="OQL90_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1370362.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2114..2548)
                     /gene="marR"
                     /locus_tag="OQL90_003744"
     CDS             complement(2114..2548)
                     /gene="marR"
                     /locus_tag="OQL90_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1370363.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2760..3425
                     /gene="marC"
                     /locus_tag="OQL90_003745"
     CDS             2760..3425
                     /gene="marC"
                     /locus_tag="OQL90_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1370364.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3450..4640)
                     /gene="ydeA"
                     /locus_tag="OQL90_003746"
     CDS             complement(3450..4640)
                     /gene="ydeA"
                     /locus_tag="OQL90_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1370365.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4790..5660)
                     /gene="yneK"
                     /locus_tag="OQL90_003747"
                     /pseudo
     CDS             complement(4790..5660)
                     /gene="yneK"
                     /locus_tag="OQL90_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         1530 a         1253 c         1387 g         1490 t
ORIGIN      
        1 gcggtagccg cgcatcagtt ggaaggtggc tggaatgaag gaggaaaagg catcagtatc
       61 gctgatgtga tgactgctgg cgctcacggg gtgccgcgtg aagtgacaga aggcgttatc
      121 gacgggctta attatcccaa tcatgaagca attgattttt atcatcgcta taaaacagat
      181 attcagttat ttgccgagat gggattcaaa tgctttcgaa cttccattgc ctggacacga
      241 atctttccgc aaggtgacga acaggagccg aatgaagagg gtttacaatt ttatgatgat
      301 ctgttcgatg aatgccttaa gcagggaatg gaacctgtgg tgacgctttc acattttgag
      361 atgccttatc atctggtgac aaaatatggt ggctggcgaa accgtaaact gatcgacttt
      421 tttatccgct tcgcatcaac ggtcttcacg tgctataaag aaaaagtaaa gtactggatg
      481 acgtttaacg aaatcaataa tcaggtgaat ttcagcgaaa gcctgtgtcc atttactaat
      541 tccggtatct tgtattcgcc agaggaagat atcaatgagc gcgaacaaat aatgtaccag
      601 gcggtacatt acgagttagt tgccagtgcc ctggcggtac agactggaaa atcgatcaat
      661 cctgaattta acattggctg tatgatcgcc atgtatccca tctatcctct gacgtgtgta
      721 cccaacgata tgatgatggc cacgaaagca atgcatcgtc gttactggtt tactgatgtt
      781 catgctcgtg gatattatcc gcaacatatg ctgaattact ttgccaggaa aggattcaac
      841 ctcgatatca caccagaaga taacgcgatt cttgccagtg gttgtgtcga ctttatcggc
      901 tttagctact acatgtcttt tacgacacaa ttttcgcccg ataacccgca actggattat
      961 gttgaaccac gagatttggt cagcaaccct tatatcgata catccgaatg gggatggcaa
     1021 attgatccgg cagggctacg ttattcactc aactggttct gggatcattt ccagttgccg
     1081 ctgtttattg tcgaaaatgg atttggtgcg gttgaccaga gacaagctga cggcacggtg
     1141 aacgatcact atcgcattga ttactttgct tcccatattc gggaaatgaa aaaagccgta
     1201 gttgaagatg gtgttgactt aattggctac accccgtggg gctgcattga cctggtttct
     1261 gccggaacag gggaaatgaa aaagcgctac ggaatgattt atgtcgataa agacaacgaa
     1321 gggaagggaa cgctggaacg gatacgtaaa gcgtcgtttt actggtatcg ggatctcatc
     1381 gccaacaatg gcgaaaatat ttgagacttg aggaagatat tacggagatg tcaggctctg
     1441 tgatcagagc ctgacaataa ctacatagcg tgttgattat aatagggcac gccgagcgca
     1501 tccgacttat cactgccagt acccatgtga tttaaatcaa acggtggttg ttcctgcgat
     1561 gggggaacaa ccacgacatt ggcacaagaa gtaacaactg gctgcgtggt ttgttccgca
     1621 acgccctgcg cggaaaagag aataagcgca gctgctattg cggatgaaag tggtttcatg
     1681 attgcctcag tgacgttgtc acgttatcaa ctagctattg taatgattta atggatgtaa
     1741 aaagcgcgat tcgccctgca tattggtcat ccggtattta tgtggcggaa catcaaagta
     1801 atttttgaag gttcgggtca gagtttgttg cgactcgaag ccatatcgtt ctgccagata
     1861 gagtatcggc tcgttacttt ccttcagctt ttgcgcgatt tccgtcatct tacggctgcg
     1921 gatgtattgg cctaatgaat gaccggtttc ttttttaaac atccgttgca ggtgccattt
     1981 agagtaacct gaacgctctg acactttctc cagtgacagt ggcgattcca ggttgtcctc
     2041 gatccagtcc aaaatgctat gaatggtaat agcgtcagta ttgcgtctgg acatcgtcat
     2101 acctcttttt tgtttacggc aggactttct taagcaaatg ctcaagtgtt gccacttcgt
     2161 ccgccgtcag gttttttgtt aattcttgat gcaggtcctg gccaactaat tgatggcatt
     2221 gttcgcatat tgccgcgccg ctggtggtaa gttttaccag tacgccgcgc ttatcattcg
     2281 ggttcggcaa cctttcaacc cagcctttac agaccaggcg atccagcata cgggtcagtg
     2341 ctccaaggtc gacagacaac acttttttca gttcaaccgg agtaatacac gccgcgcagc
     2401 ggatagagca gagcacctta aactgtgccg cggtaatatc cagcggagac agatactcgt
     2461 taagcagacg atctttcttc tgattaacca tatggattaa gcgacccaat ggaataattt
     2521 cattgaacag atcgctggta cttttcacat tagttgccct ggcaagtaat tagttgcagg
     2581 gggtaatatt gcccaggcaa gtataagtca aataaatgaa ttgaccgatg ccacgttttg
     2641 ctaaatcggt tcaagttttg gtcaatgcga tgcaaggtta gctaaagagt gacactttat
     2701 ccttgtttac cccttatact tttcgctgat aatccagata cactggataa caaccaccaa
     2761 tgttagattt gtttaaagca attggcctgg ggctggtggt gttactgccg ttagctaacc
     2821 cattaacaac tgtcgcgtta tttcttggcc tggcaggcaa catgaacagt gccgaacgta
     2881 atcgtcagtc gttgatggcc tcggtgtacg tatttgccat catgatggtg gcgtattacg
     2941 ccgggcaact ggtgatggat acatttggca tttcgattcc cggtctgcga attgcaggcg
     3001 gcttaatcgt cgcgtttatc ggttttcgga tgctctttcc gcaacagaaa gcgattgatt
     3061 caccggaggc gaaaagcaag tcggaagagc tggaagatga acccagtgcc aatattgcct
     3121 ttgtgccgct agcaatgcca agtaccgctg gtccgggaac catagcgatg attattagct
     3181 cggcgtccac agtgcgtcag agttcaacct tcgctgattg ggtactgatg gttgcgccgc
     3241 cgctgatctt tttcctggtg gcggttattt tgtggggaag cctacgcagc tccggcgcaa
     3301 ttatgcggct ggtgggcaag ggtggcattg aagcgatctc ccgtctgatg ggattcctgc
     3361 tggtatgtat gggtgtgcag tttattatta acggcatcct ggaaatcatt aaaacgtacc
     3421 attaaaaaag gcccgaatgg gcctttcaac tattgcgtct gttcttcgag tgtcactggc
     3481 cagcggcgaa atataatgat tgaccatatt aacgcggcaa aagcaggcac cgcgcccaca
     3541 taaccaatca tcgacattga ccagtgcaaa ctcacctgat tacctaccaa cgcacccgcc
     3601 ccgattccaa tattaaatat gccggagaat agcgccatcg cgacgtcggt agcatctggt
     3661 gccagcgcca gcactttaac ctgcatacca agcccgatga tcatcatcgc gatcccccag
     3721 aaaatactca gcaccccgag gtgtatttca ctgttcgccg caggtagcag cagtgccaga
     3781 cacaccagca acagcgcaat cgccgtactc accaacgcag acgcatactg attacccagt
     3841 ttaccgaaaa tcacgctgcc aataatgccc gcaccaccga gtaataacag taatgccgtg
     3901 gcaaagttgg cgctgaatcc cgcaatgttt tgcacaaaag gctcgatata gctgtatgcc
     3961 gtgtaatggg cggtgacaac caccacagtt aacaaataaa tgctcatcaa tgccgggcgg
     4021 cggaacaata gcgggaggct tttcaatgaa ccggaatgct cactgggcag taagggaagt
     4081 aacttaatca ggcacaaaag ggtgataagc gccccaatac caatcgcgaa gaaggtcatt
     4141 cgccaaccga aatactgccc cacaatgcgc ccgagaggta aacctaagac catcgccagt
     4201 gctgtaccgg tggcaattaa actcaaggcc tgtgcccgct tcccggccgg agccatacgg
     4261 atcgccagag acgccgtaat cgaccagaaa atcgcatgtg caaaagccac accaatgcga
     4321 ctgatcacca gaacggtaaa gctccacgat aaaaacgaca gtacgtggct ggcaataaac
     4381 accacaaaca ggcagatcag taatttgcgc cgttcaacct gactggtcat taacataaaa
     4441 ggcaatgaca ttagcgctac tacccatgcg taaatggtca acatgatgcc gacctgagcg
     4501 gtttgcatgt gaaaactttg cgcaatgtca gagagcaggc caacagggac aaattcggtg
     4561 gtgttgaaga tgaaggcggc gactgccagc gtaacgaccc gtagccacgc cactttgcgg
     4621 gaaacagtgt ttgttgtcat aacagattat cgggtgagtt gcgaaaaagt gatgaaatga
     4681 tcttaaaacg gttaatgatg tgaatacaat gcatttgtga tctggatcgc gttttccgtt
     4741 tctctgacgc tgattctgtt ccgcgtcaga gagtcggtaa catcacggtt taatgacgtt
     4801 cagggaggtc tggcgttttg gcggttgcgg gtataggtgg tctgacaggg gagcgaggcc
     4861 tgtttttcac caggtcttcg tgagtaactt ttggcggcgt ttcatttttt acaaacagtt
     4921 tgagggtcct gcgcgccata tgcttgataa ttttatcatc acttaatgat gctggcatca
     4981 acacgtcatt cagatcactt tcattgggat tcataaattg gtgatagtgc aataacatat
     5041 tggcgaaaaa taaaaaaaag tcctgaaaat gaggagattc ttgttctgct aaaagggata
     5101 aaaagcagat gcagacttcg ggtagtacat gtttgttaga acctaatgaa tccatcaggc
     5161 tttgcaataa atcctctttt aattcgccgt ctgttatgtc gagaatggcg gtgatcaagt
     5221 taggatcttt attgacgaac catctgtgaa cctcagagtt actccataat cgggggagta
     5281 tgacaaaatt accgcgagag ataaaattca ggacaagcaa atcaaactgg cgtaagtaat
     5341 ctgcagcggt atggtgatcc ggaggcaggg gttcaatatg tggtgtatgc gcttttttga
     5401 tggggacgcc aaggtggatc attggaaatt gttcaataaa atcattcaga aacagaaaga
     5461 gcacacgctc gacgccggtg tgaaatacgc gccccatagt gacaggcttt gggttgggag
     5521 cgcaaaaata ttaatttctc catcatggcg aaaattatct aaataaatag ggtttaaatt
     5581 tattactacc tgaaattcaa tattttcttt gtgtcttcca ttcttgacgg gataagtgag
     5641 tttaaactga accttttcat
//
LOCUS       DAJVOY010000217         5638 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVOY010000217 DAJVOY010000000
VERSION     DAJVOY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.219"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..509
                     /locus_tag="OQL90_003748"
     CDS             <1..509
                     /locus_tag="OQL90_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1370366.1"
                     /translation="GFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGTVVRRKV
                     YAHFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGR
                     IMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGF
                     LSINKLSQ"
     gene            515..1258
                     /locus_tag="OQL90_003749"
     CDS             515..1258
                     /locus_tag="OQL90_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370367.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            1195..1827
                     /locus_tag="OQL90_003750"
     CDS             1195..1827
                     /locus_tag="OQL90_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1370368.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKDRTPRTQTTDNGKQN
                     TCFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1888..5286
                     /locus_tag="OQL90_003751"
     CDS             1888..5286
                     /locus_tag="OQL90_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1370369.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRVLQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGAGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGNVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            5353..>5638
                     /locus_tag="OQL90_003752"
     CDS             5353..>5638
                     /locus_tag="OQL90_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1370370.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNR"
BASE COUNT         1284 a         1427 c         1754 g         1173 t
ORIGIN      
        1 gcggttttga actgaatggc aaaggcacca gtacgcgccc cacgctgacg gtttctaacc
       61 tgtacggtat ggtcaccggg atggcggaag atatgcagag tctggtcggc ggaacggtgg
      121 tccggcgtaa ggtttacgcc cattttctgg atgcggtgaa cttcgtcaac ggaaacagtt
      181 acgccgatcc ggagcaggag gtgatcagcc gctggcgcat tgagcagtgc agcgaactga
      241 gcgcggtgag tgcctccttt gtactgtcca cgccgacgga aacggatggc gctgtttttc
      301 cgggacgtat catgctggcc aacacctgca cctggaccta tcgcggtgac gagtgcggtt
      361 atagcggtcc ggctgtcgcg gatgaatatg accagccaac gtccgatatc acgaaggata
      421 aatgcagcaa atgcctgagc ggttgtaagt tccgcaataa cgtcggcaac tttggcggct
      481 tcctttccat taacaaactt tcgcagtaaa tcccatgaca cagacagaat cagcgattct
      541 ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgcggc ttcgtggtaa gcacgccgga
      601 gggggaaaga tattttccct gcgtgaatat ctccggtgag ccggaggcgt atttccgtat
      661 gtcgccggaa gactggctgc aggcagaaat gcagggtgag attgtggcgc tggtccacag
      721 ccaccccggt ggtctgccct ggctgagtga ggccgaccgg cggctgcagg tgcagagtga
      781 tttgccgtgg tggctggtct gccgggggac gattcataag ttccgctgtg tgccgcatct
      841 caccgggcgg cgctttgagc acggtgtgac ggactgttac acactgttcc gggatgctta
      901 tcatctggcg gggattgaga tgccggactt ttatcgtgag gatgactggt ggcgtaacgg
      961 ccagaatctc tatctggata atctggaggc gacggggctg tatcaggtgc cgttgtcagc
     1021 ggcacagccg ggcgatgtgc tgctgtgctg ttttggttca tcagtgccga atcacgccgc
     1081 aatttactgc ggcgacggcg agctgctgca ccatattcct gaacaactga gcaaacgaga
     1141 gaggtatacc gacaaatggc agcgacgcac acactccctc tggcgtcacc gggaatggca
     1201 cgcatctgcc tttacgggga tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa
     1261 acgggggctg aagccatccg ggcactggcc acacagctcc cggcgtttcg tcagaaactg
     1321 agcgacggct ggtatcaggt acggattgcc gggcgggacg tcagcacgtc cgggttaacg
     1381 gcgcagttac atgagactct gcctgatggc gctgtgattc atattgttcc cagagtcgcc
     1441 ggggccaagt caggtggcgt attccagatt gtcctggggg ctgccgccat tgccggatca
     1501 ttctttaccg ccggagccac ccttgcagca tggggggcag ccattggggc cggtggtatg
     1561 accggcatcc tgttttctct cggtgccagt atggtgctcg gtggtgtggc gcagatgctg
     1621 gcaccgaaag acagaactcc ccgtacacag acaacggata acggcaaaca gaacacctgt
     1681 ttctcctcac tggataacat ggttgcccag ggcaatgttc tgccggttct gtacggtgaa
     1741 atgcgcgtgg ggtcacgcgt ggtttctcag gagatcagca cggcagacga aggggacggt
     1801 ggtcaggttg tggtgattgg tcgctgatgc aaaatgtttt atgtgaaacc gcctccgggc
     1861 ggttttatcg tttatggagc atgacgaatg ggtaaaggca gcagtaaggg gcataccccg
     1921 cgcgaagcga aggacaacct gaagtccacg cagctgctga gtgtgatcga tgccatcagc
     1981 gaagggccgg ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa cagtacgccg
     2041 gtgctggaca gtgaggggaa taccaatata tccggcgtca cggtggtgtt ccgggccggt
     2101 gagcaggagc agacaccgcc ggagggattt gaatcctccg gttccgagac ggtgctcggt
     2161 acagaagtga aatatgacac gccgatcacc cgcaccatta cgtctgcaaa catcgaccgt
     2221 ctgcgcttta ccttcggtgt acaggcactg gtggaaacca cctcaaaggg ggacaggaat
     2281 ccatcggaag tccgcctgct ggttcagata cagcgtaacg gtggctgggt gacggaaaaa
     2341 gacatcacca ttaagggtaa aaccacttca cagtatctgg cctcggtggt ggtggataac
     2401 ctgccgccgc gcccgttcag tatccggatg cgcaggatga cgccggacag caccacagac
     2461 cagctgcaga acaaaacgct ctggtcgtca tacaccgaaa ttatcgatgt gaaacagtgc
     2521 tacccgaaca cggcactggt cggcgtgcag gtggactcgg agcagttcgg cagccagaag
     2581 gtgagccgta attatcatct gcgcgggcgt gttctgcagg tgccgtcgaa ttataacccg
     2641 cagacgcggc aatacagcgg tatctgggac ggaacgttta aaccggcata cagcaacaac
     2701 atggcctggt gtctgtggga tatgctgacc catccgcgct acggcatggg gaaacgtctt
     2761 ggtgcggcgg atgtggataa atgggcgctg tatgtcatcg gccagtactg cgaccagtca
     2821 gtgccggacg gctttggcgg cacagagccg cgcatcacct gtaatgcctg gctgaccacg
     2881 cagcgtaagg cgtgggatgt gctcagtgat ttctgctcgg cgatgcgctg tatgccggta
     2941 tggaacgggc agacgctgac gttcgtgcag gaccgaccat cagataaggt gtggacctat
     3001 aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag cttcagcgcc
     3061 ctgaaggacc gccataatgc cgttgaggtg aactggattg acccggataa cggctgggag
     3121 acggcgacag agcttgtgga ggacacgcag gccattctcc gttacggtcg taacgtcacg
     3181 aagatggatg cctttggctg taccagccgg gggcaggcac accgcgccgg gctgtggctg
     3241 attaaaacgg agctgctgga gacgcagacc gtggatttca gcgtcggcgc agaagggctt
     3301 cgccatgtgc cgggtgatgt tattgaaatc tgcgatgatg actatgcggg gatcagcatc
     3361 ggcgggcgcg tgctggcggt gaacagccag acccggacgc tgacgctcga ccgtgaaatc
     3421 acgctgccat cctccggtac cacgctgata agcctggttg acggaagtgg caatccggtc
     3481 agcgtggagg tccagtccgt caccgacggc gtgaaggtaa aagtgagccg tgttcctgac
     3541 ggcgttgccg gatacagcgt atgggggctg aagttgccga cgttgcgcca gcgcctgttc
     3601 cgctgcgtga gtatccgtga gaacgatgac ggcacgtatg ccatcaccgc cgtgcagcat
     3661 gtaccggaaa aagaggccat cgtggataac ggggcgcact ttgacggcga ccagagcggc
     3721 acggtgaatg gtgtcacgcc gccagcagtg cagcatctga ccgccgaagt cactgcagac
     3781 agcggggaat atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa gggcgtgagc
     3841 ttcctgcttc gcctgaccgt ggcagcggat gacggcagtg agcggctggt cagcacggcc
     3901 cggacggcgg aaaccacata ccgcttcacg cagctggcgc tgggacggta cacgctgaca
     3961 gtccgggcgg taaatgcgtg gggacagcag ggcgatccgg cgtcggtatc gttcaggatt
     4021 gccgcaccgg tagcaccgtc gcggattgag ctgacgccgg gctattttca gataactgcc
     4081 acgccgcatc ttgcggttta tgatccgacg gtacagtttg agttctggtt ctcggaaacg
     4141 cggattaccg atatcaggca ggttgaaacc acagcccgct accttggcgc ggggctgtac
     4201 tggatagccg ccagtatcaa tatcaaaccg ggccatgatt attactttta tatccgcagt
     4261 gtgaacaccg ttggcaaatc ggcattcgtg gaggccgtcg gtcgggcgag cgatgatgcg
     4321 gaaggttacc tggatttttt caaaggcaag ataaccgaat cccatctcgg taaagagctg
     4381 ctggaaaaag ttgatctgac ggaggataac gccagcagac tggatgagtt ttcgaaagag
     4441 tggaaggacg ctaacgataa atggaatgcg atgtggggcg tcaaaattga gcagaccaaa
     4501 gacggcaaac attatgtcgc gggtattggc ctcagcatgg aggacacgga ggaaggcaag
     4561 ctgagccagt ttctggttgc cgctaaccgt atcgcgttta ttgacccggc aaacgggaat
     4621 gaaacgccga tgtttgtggc gcagggcaac cagatattca tgaatgacgt gttcctgaaa
     4681 cgcctgacgg ctcccaccat taccagcggt ggaaatccgc cggcattttc cctgacacca
     4741 gacggaaagc tgaccgctaa aaatgcggat atcagcggta atgtgaatgc aaattcaggg
     4801 acgctcaaca atgtcacgat taatgaaaac tgtcagatta aggggaaact gtcagccaat
     4861 cagattgaag gcgatattgt caaaacggtc agcaagtctt tcccccgcac gaacagttat
     4921 gccagtggca ccatcacggt aagaatcagt gatgatcaga aatttgaccg gcaggtcatg
     4981 ataccgccag tgttattccg cggtggtaag catgagaatt tcaacagtaa taaccaacag
     5041 tcatactggt attcaacctg ccggttaaga gtgacccgca atggtcagga gatttttaat
     5101 cagtccacga cggatgctca gggcgtattt tcctcagtta tagatatgcc tgccggacag
     5161 gggacgctga cactgacatt caccgtatct tcatcaggag cgaataactg gacaccaaca
     5221 accagtatca gcgatctgct ggttgtggtg atgaaaaaat ccacagcagg tatcagtatc
     5281 agctgaattt tataacccag aacgggcgtc agaaatgacg ccttttttat tgcagaaaag
     5341 cgagaggtaa ttatgcgtaa actttatgcc gccattttgt ccgcagccat ttgtctggcc
     5401 gtatccggtg cgcctgcatg ggcgtctgaa catcagtcca cgctgagcgc ggggtatctt
     5461 catgcccgga cgaacgttcc cggcagtgat gatctgaacg ggattaacgt gaaataccgt
     5521 tatgagttta cggacacact ggggatggtg acgtcgttca gctatgcagg agacaagaat
     5581 cgccagatta cccgttacag cgatacccgc tggcatgaag attccgttcg taaccgct
//
LOCUS       DAJVOY010000218         5591 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVOY010000218 DAJVOY010000000
VERSION     DAJVOY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.220"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OQL90_003753"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OQL90_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1370371.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OQL90_003754"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OQL90_003754"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1370372.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OQL90_003755"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OQL90_003755"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1370373.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OQL90_003756"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OQL90_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1370374.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1391 a         1337 c         1515 g         1348 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcggca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaaa
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc gcttgtcagg ccggaataac tccctataat
     5461 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
     5521 gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcgggaaag cgcattatgt
     5581 acaccccgcg c
//
LOCUS       DAJVOY010000219         5338 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVOY010000219 DAJVOY010000000
VERSION     DAJVOY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.221"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(891..1832)
                     /gene="fabY"
                     /locus_tag="OQL90_003757"
                     /gene_synonym="yiiD"
     CDS             complement(891..1832)
                     /gene="fabY"
                     /locus_tag="OQL90_003757"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1370375.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1877..2314)
                     /gene="dtd"
                     /locus_tag="OQL90_003758"
     CDS             complement(1877..2314)
                     /gene="dtd"
                     /locus_tag="OQL90_003758"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1370376.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2311..3184)
                     /locus_tag="OQL90_003759"
                     /pseudo
     CDS             complement(2311..3184)
                     /locus_tag="OQL90_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3178..3777)
                     /gene="yihX"
                     /locus_tag="OQL90_003760"
     CDS             complement(3178..3777)
                     /gene="yihX"
                     /locus_tag="OQL90_003760"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1370377.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3918..4805)
                     /locus_tag="OQL90_003761"
     CDS             complement(3918..4805)
                     /locus_tag="OQL90_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1370378.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4795..>5338)
                     /locus_tag="OQL90_003762"
     CDS             complement(4795..>5338)
                     /locus_tag="OQL90_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1370379.1"
                     /translation="GIVYISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQK
                     SLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGT
                     KVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIE
                     PTDEQETASYRYIREQSHEN"
BASE COUNT         1441 a         1346 c         1253 g         1298 t
ORIGIN      
        1 ctgataaatg aagaaaaacc gtattcatcg ccattaccac caccttacct atattactca
       61 ttaatgtatg tgcgaaaatt acgccggaaa atcattaaaa aaaagcggat ttttgcatgt
      121 tgttttgttt ttgcgaggcg ttttccagaa ataataagag tgttgcagtt gtttcattta
      181 ttactcaaaa atggtttagt tatcgcccag gatgaattat gggtgctatg aatatattat
      241 attcagagat atttatttgc ataatctgaa tggtgtagcc gaaaaacgtc agcatcctaa
      301 cagcaccagg atttaaggtg aaattaatct ttcatcacag accggtcagt actgccacca
      361 accggtcttt acttgctcat cgttataatt ttgttggtaa cagttcggga aagttgtttt
      421 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      481 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      541 gtaaatgaat gatactgaca atgatgccag cccattatct ttgcataatg tttgtgagct
      601 atagatttta aaaatattta tgaggaaagt atggcaccag aggaggtttt gactatcaaa
      661 aatcgctcaa acctgaacta agatgatgag ttaagcatta tctgcgataa ttgccactca
      721 gctctacaaa taatatttaa atgctcctga acagtgatgt agcaagacac gtatggtgcc
      781 aacgaagaat aaaaatttat agagttctta ttcaatatta ccggcataga cagggaatga
      841 tgtaaatgac cggaatgaat gagagaggca gcaccgcccc tctccataag ctactcttct
      901 tcgttcccgc cctcttcata cgggccaaat ggcttcgcgg gcagaacgat atacgtgcct
      961 tcaaacactg cacccggcgt ctcgtcgcca aagatttcaa cttgcatctg cacccgtgct
     1021 tttcgtccgc gcgccagacg gtcgagatcg ccgcttaagg caccaaggtc ggctaccgca
     1081 tgaggtttac cgctaatcgg cttgctgtag cggatatgcg catcggccag aataatcgtt
     1141 ccgccgaggt ggcgttcgcg cagcatcagc cagataagcc cccaaccggt gagcgttgcc
     1201 agcgagaata aactcccggc aaacagcgta tggtgcggat tctgattacc ggtttccggc
     1261 atggtggtga taaatttttg cccggtatat tgctgaatgc gcacgcccat tttttcacta
     1321 agcgggatgt gttcgtacca cgcctgttgc agctgcgcgc accagtcgcc gcgatgcaaa
     1381 atatcatcca gagtggcaac aggtttaatc atcaaaaaat ggcgaatcgg cgtggtggtt
     1441 ggtgtggtga tttctccctg attaacaaac cccagcttgg cgaaaaactc caccgcgtct
     1501 tcacgggcgc tacaggtcac gcgcttaacg ccttcctgac gcgccaccga ctccagggtc
     1561 atcgccatca acgtccccag ccctttgtcc tgcacgtcgg gatgaacggc cataaagcga
     1621 atggacgctt cattgtcggc attaatatac agtcggccta ccgccaccag attcccctgc
     1681 tcgtcgacga ccatctgatg atgcgccatc gcatcccacg cgtcgcgttc cgaacctttt
     1741 ggttgatgca ggggcttacg caacatttcc cagcgaaact gatagtaacg ctctaactct
     1801 tcttctgttt gtggaacccg aaggtgatac atagcgatac tctctcttgt ttcccgtgac
     1861 aaccctggaa gctggctcat acctgcaacc agaatgtcac ggggccatca ttgaccagcg
     1921 atacctgcat atcggcagcg aagcgtcctg tttgcgtgtt catctcttgc tggcggcagc
     1981 gttcgacgaa atagtcatat aacgcctctg cgcgatccgg tgatgcgcct ttggagaaac
     2041 ttgggcgcat cccccgttcg gtatctgcgg cgagggtaaa ctgggaaacc accagtacac
     2101 tgccgcccgc ctgttgcacg ttgagattca tcttgccttc ggcatcgcta aagatgcggt
     2161 agccgagcac acgctcgcac agacggtttg ctttctgttc gtcgtcatcc ttttcgacac
     2221 ccaataacac caaaagtccc gcgccaattt cgcccgtcac ttctccctcc acggtgacgc
     2281 tggcacgggt tacgcgttga attaatgcaa tcatggttcg tcgtcttctt cttgttcagc
     2341 tgcttgtttt agtttgcggt attccccgag agtgacagta atttccgcgc caagcaagac
     2401 gatacaccat gtccagtaga cccaaacaaa gagaatgggg atcaccgcca gcacaccgta
     2461 aatgagctga tatgacggga acatggtgat ataaagcgcg aaacctttct ttcctgcttc
     2521 gaacaggagt gcggcgacaa acgcgccgac aatcgcgtcg cggttaggta cgcggatggt
     2581 aggaacaatg ctgtacagca accagaagga gatccacgac aacagcaacg gaaaaatacg
     2641 cagcacgttg tcgatgacag tattgagatc gctcgcccag cgcagggaga gcaaatagga
     2701 actgatcgcc agactggccc ctgccagcag cggccccagc gttaaaatca tccagtacac
     2761 ggcgaacgag taaatttttg gtcgcgctcg tttactgcgc cagatggtat tcaacgcgct
     2821 atcgatggag tacatcaaca ataacgcggt gacgatcagc ccgcacgccc caacggcggt
     2881 catcttgttg gaattggcaa caaattgttc gatataccgc tgaataacat cgccagtagc
     2941 aggcagaaag ttggcaaaaa taaagtgacg taactgaatg ctgacgtcgg aaaacatggg
     3001 aaaagcggcg aaaagcgcaa aaacaacggc aaccagcggc actaatgaga gcaacgacca
     3061 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     3121 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     3181 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     3241 aatgcccagc tgattggctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3301 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3361 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3421 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3481 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3541 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3601 ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3661 atgaaaactc ttcttaagcg tagccagtgg aacacgcgtt aaatcgctcc aggctcccag
     3721 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3781 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3841 gcaataaggg taatcttgtg atgctgattg cgaaaaagca gagtaatttc actggctttt
     3901 tcgcaataga tgaatggtta agattcaggt gtaaattgcc aacgagtttc gatgaccagc
     3961 ggcctatctg gtgtcaccaa ttccggcgtt agatttattg cgttaggtgc ctgagtcagc
     4021 gggttaatac aggtggcatc gggctgttta tcaaatacca ccagactgtt tgcagaagag
     4081 atcatggtca tcgcgagttt accaggccac agtaacttaa ccttcacccc gttataaaaa
     4141 ccaaaacagt catcccaggg accggcttga ggagagatac gatttccagt tggcagttca
     4201 tcactacccg cttcttcttg ccagtccgca tcaaaaagca cctgcaacga ttctgtattt
     4261 tgcggagtca gctttttcgc aaaccacgga tgccagccag cggaggccgg gaatgtatcg
     4321 gcatatgaat ggacttccag ttgcaaaacc agcgcatcgt tctccagcaa aaaagtctga
     4381 atcacttccc cttgccaggg ccacggtgaa gccaggggca tacgtaaagt cagggaatct
     4441 gctgttttgt cgatgatttc ccacgttgag taacaagcca taccatgcaa cgcatgtggt
     4501 ggtttattgg cgggtaaaga ataacattgc ccaccagcat tcagcgtcgc attccccagt
     4561 cgccctgccc acggcaccat tgggaaacag ccatactgaa aagctctgcg ctgcggctgc
     4621 cattggcgta atacttcaga accaaatgcc tttaacgagg ttatcctggc accatcatgc
     4681 ggataaatct gcaggtgaat attgcctttt tccagcgtca ctaactctcc tacaggccca
     4741 ggccatggcg cgtcaaacgc ctgttgtagc gccaggtaat gctcgttttt tcgttcagtt
     4801 ttcatgagat tgttccctga tgtagcggta agaagcggtt tcttgttcat cggtcggctc
     4861 aatcagccag acgcaaacaa aggaaagaac tgagacagca aataaaaaca gagcgacata
     4921 ccacggtttt cctcccccta gcgccagcaa acttgaggcg ataagggggc ttagtccgcc
     4981 aagaatcgcg gaaaattgat aagcaaaaga aaggccggta tatcgtactt tggtaccgaa
     5041 catgcgggtg aaaagtgttg gctgcacggc aaacatcatc gtcggaccta agttgtagcc
     5101 gaggatcata ctgcaccaga tgatgagcgt acttttgcta tccagtaata agaagaaagg
     5161 aaacgcgaac aggacacaaa atcctgctcc cgacaaatac agactttttt gacctatacg
     5221 atctgataac catccaacca atggacaact gaaaataccg atcgccgagg caatcaacat
     5281 acccgtcagg ggaatatctc gcgacaatac taattggctg gaaatataga caatacca
//
LOCUS       DAJVOY010000220         5332 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVOY010000220 DAJVOY010000000
VERSION     DAJVOY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.222"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..1638)
                     /gene="dhaR"
                     /locus_tag="OQL90_003763"
     CDS             complement(<1..1638)
                     /gene="dhaR"
                     /locus_tag="OQL90_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCS1370380.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSV"
     gene            1866..2975
                     /gene="dhaK"
                     /locus_tag="OQL90_003764"
     CDS             1866..2975
                     /gene="dhaK"
                     /locus_tag="OQL90_003764"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCS1370381.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWDKLGESNVTEQNSNC"
     gene            2947..3579
                     /gene="dhaL"
                     /locus_tag="OQL90_003765"
     CDS             2947..3579
                     /gene="dhaL"
                     /locus_tag="OQL90_003765"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCS1370382.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3590..5008
                     /gene="dhaM"
                     /locus_tag="OQL90_003766"
     CDS             3590..5008
                     /gene="dhaM"
                     /locus_tag="OQL90_003766"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1370383.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDYIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1342 a         1355 c         1325 g         1310 t
ORIGIN      
        1 gacgctgtaa agttcaaaat cgttgcctgg ccatgcacaa gaaaccaggc gagcgagggc
       61 atcgtcatca attttcagcc gcgtagagaa gcgtttttca agactgcgta atttgttatt
      121 caccagcgcc ggaatgctgc cacgccgcat acgcagaggc gggatggtaa tttcaaatgc
      181 atgcagcgcg taatacagct ggcgactaaa acgattttgt tccaccagca ttgcgaggtc
      241 cgcggtcgtt gttgcaatca ctttgacatc aattggtatt aaacgccgcg catccagtcg
      301 cgtgataaca ccctgcttga taacctgaag caaagcagac tgtaactcca ccgccagata
      361 ttcaatcttt tcaagaaaca gcgtgccgcc gtgcgccagt tccagccgac tcagacggcc
      421 attttcattg tccgtacgat cgccaccaat aaattcttcc gccagtgccg catcaccata
      481 taactcacaa ttgacggcga tataaggacc tgcagcacgc tcgctttcat tatgaattgc
      541 ctgacttagc agtgccttgc ccacgccctc ttctccacaa agcaggacag gaaagctact
      601 gcgcgccgcc tggcgaccaa aatgaatcaa gcggcgggtt tgcggatcgt cctgtggcat
      661 atgagcaaag gtatggctga cttttcctaa ttgactggtc atcaactgcc gcatctgttc
      721 cacaggatgg agcaacaaaa taaagctggt tccctgcgtt tctattatcg gtttaagggt
      781 tatcaccgca tcaataaact gatgctgact ttcaaaggtt gcttctacgt gtttgagcgg
      841 atgtgcctgt tttattgctt gttgcaatac ggcgggtaac gttaagagtt cagtgattgc
      901 ccgtccctga cttgccgtcg cgtcaaggcg caagacccgc gccgcctggg cattgataaa
      961 ttgcaaatta ccctgctcgt cccagctaat cacgccatca tccatacttt ctaacagggc
     1021 attaagttga tttaaatgac ggttagtttc agcgagcaaa ctgtccgtca gcagtaaatt
     1081 tccgacctcg cgggcgattg ccaacgtcaa cggcaaatca gctgcggtag tttgctcaac
     1141 cggacacgcc agcgctattg ttcccgtcaa tcggcccttg ctgtcaaaca acggcgttgc
     1201 acaaaaggcc cagttccaga gtgcctgttt gaaatgttga tcggccatcg ttttcacggc
     1261 ctgaccagag atagccgcta acgatagcgc acaagtacca ataattccct cggcgcaata
     1321 cgtgccgtca tggaatccaa gtgcacttag ctgctgcaag gtttgcggat cgccattacg
     1381 gctgagaatg caggcggttt catcgaggat aaacagcgca cactctcgcg gtgccatata
     1441 ttcccaggca tcttccagcg cagcctgccc gagcgtcagc atcgctttct tacgccgata
     1501 aatagaagca aatgtcacgc cctgggcctg atgtggtacg ctccatgtct cccgtttcat
     1561 cagcttattg catcgctccc aggaggttgc aattaaggga gatatgcccc gaccatcgtt
     1621 gttaaaagcg ccactcatat cccgcatccc ttatgacaaa aggtgttccg gctcgcaatt
     1681 ttaagtatta atacacgtaa aagtttcaat atggaacatt cattaaagca tatgtttcac
     1741 tatgaaacat aatttgggct aattttcttt tcccttgcct gatgcacaat ggattcatcg
     1801 gcagtcagtg gtcgccgtgt cgttgaacat catccatgcc ctaccgtaat tgctggagca
     1861 aaataatgaa aaaattgatc aatgatgtgc aagacgtact ggacgaacaa ctggcaggac
     1921 tggcgaaagc gcatccatcg ctgacactgc atcaggatcc ggtgtatgtc acccgagctg
     1981 atgcccctgt tgcaggaaaa gtcgccctgc tgtcgggtgg cggcagcgga cacgagccga
     2041 tgcactgtgg gtatatcggt caggggatgc tttcgggggc ctgtccgggc gaaattttca
     2101 cctcaccgac gcccgataaa atctttgaat gcgccatgca agttgatggc ggcgaaggtg
     2161 tactgttgat tatcaaaaat tacaccggcg atattcttaa ctttgaaaca gcgaccgagt
     2221 tactgcacga tagcggcgta aaagtgacca ctgtggtcat tgatgacgac gttgcggtaa
     2281 aagacagtct ttatactgcc gggcgacgcg gcgttgccaa caccgtatta attgaaaaac
     2341 tcgtaggcgc agcggcggag cgtggcgact cactggacgc ctgtgcggaa ctggggcgta
     2401 agttgaataa tcaaggccac tcaataggta tcgctctcgg tgcctgtacc gttcctgccg
     2461 cgggcaaacc ttcttttacc ctggcggata atgagatgga gtttggcgtc ggcattcatg
     2521 gtgagccggg tattgaccgc cgctccttct cttcccttga tcaaaccgtc gatgaaatgt
     2581 tcgacaccct gctgggaaat ggctcatacc atcgcacttt acgtttctgg gattatcaac
     2641 aaggcagttg gcaggaagaa caacaaacca aacaaccgct ccagtctggc gatcgggtga
     2701 ttgcgctggt taacaatctt ggcgcaactc cgctttctga gctgtacggc gtctataacc
     2761 gcctgaccac acgttgccag caagcgggat tgactatcga acgtaattta attggcgcgt
     2821 actgtacctc actggatatg accggtttct caatcacctt actgaaagtt gatgacgaaa
     2881 cgctggcact ctgggacgcc ccggtccaca ccccggccct taactgggat aaattaggag
     2941 aaagcaatgt cactgagcag aactcaaatt gttaactggc tcactcgttg tggcgatatt
     3001 ttcagcaccg agagcgagta tcttaccgga ctggatcgcg aaattggcga cgctgaccac
     3061 gggctaaata tgaaccgagg ctttagcaaa gtggtggaaa aactccctgc tatcgcagat
     3121 aaagatatcg gtttcattct caagaatacc ggtatgacgc tgctttccag cgtcggtggt
     3181 gccagtggtc cgctgttcgg taccttcttt atccgcgccg cacaggcgac ccaggcacgg
     3241 caaagcctga cactggaaga gctttatcag atgttccgcg atggcgcgga cggcgtaatc
     3301 agtcgcggga aagccgaacc tggcgataaa accatgtgtg atgtgtgggt gccggtggtg
     3361 gaatcgttac gtcagtccag cgagcaaaat ctctctgttc cggtggcgct cgaagctgcc
     3421 agtagcatcg ccgaatccgc tgcacaaagt acgattacga tgcaagcccg caaaggccgc
     3481 gccagttatc tcggtgaacg cagtattggt caccaggatc ccggcgcgac ctcggtgatg
     3541 tttatgatgc aaatgttggc gttagccgca aaagagtaag gaattggtga tggtaaacct
     3601 ggtcatagtt tcacatagca gccgactggg agaaggtgtc ggtgaattag cccgtcagat
     3661 gttaatgagt gatagttgta aaatcgccat tgccgcggga attgacgatc cacaaaatcc
     3721 tattggtacc gatgccgtca aagtgatgga ggccatcgaa tctgttgctg atgccgacca
     3781 tgtgctggtc atgatggata tgggtagcgc attattgagt gctgaaactg cgctggaatt
     3841 gctggctccc aagatcgccg caaaagtacg tttgtgtgct gcgccgttgg tcgaaggtac
     3901 actggcagca acggtcagcg cggcctcggg agcggatatc gacaaagtta tctttgacgc
     3961 tatgcatgcg ctggaagcca aacgtgaaca actgggttta ccgtcctccg acactgaaat
     4021 ctctgacaca tgtcctgcgt acgatgaaga agcccgttct ctggcggtgg tcataaaaaa
     4081 ccgtaacggc ctgcatgtac gtccggcctc ccggctggtt tataccttat cgacatttaa
     4141 tgccgatatg ttgctggaaa aaaacggcaa atgcgtcaca ccagagagta ttaaccagat
     4201 tgcgttacta caagttcgct ataacgatac gctgcgcctg attgcgaaag ggccagaagc
     4261 tgaagaggca ctgatcgctt tccgtcagct ggctgaagat aactttggtg aaacggagga
     4321 agtcgctcca cctactctgc gtcccgttcc gcctgtttcg ggtaaagcct tttattatca
     4381 accagtttta tatacggtac aggcaaaatc aaccctgacc gtggaagaag aacaagatcg
     4441 attacgccag gctattgact tcacgttatt agatctgatg acgttaacag cgaaagcaga
     4501 agccagcggg cttgactata ttgccgcaat cttttctggt caccatacac tgttagatga
     4561 tccggaactg ctggcggcgg caagcgaact ccttcagcat gaacattgca cggcagaata
     4621 tgcctggcag caagttctta aagaacttag ccagcaatac cagcaactgg atgatgaata
     4681 tctacaagct cgctatattg atgtggacga tcttctgcat cgcaccctgg tccacctgac
     4741 ccaaacgaaa gaagaactcc cgcagtttaa ctcgccaact attctactgg cggagaacat
     4801 ttatccttcc acagtactgc aactggatcc ggcggttgta aaaggtatct gccttagcgc
     4861 cggaagtccg gtatcccaca gcgccctaat cgcccgtgaa ctggggattg gctggatttg
     4921 ccagcagggt gagaaactgt atgcgataca accagaagaa acgctaacgc tggacgttaa
     4981 aacgcaacgt ttcaaccgtc agggttaagc cactgttcgg atggcatcgt tctgatgtca
     5041 tccggcaatt tactcagcac taatggtttt taactacctg ttatattttt caccaattta
     5101 tgatgatttt tcttcgctaa tcacgaaccc aacgccgctg gctggctaat aaacagacgt
     5161 tatttgatca ctctatttcg ccgttattta tccctttaat tcccttttct aaaatgcctg
     5221 acagttcgca gaatgagatt tcgatcatgc agctagtgcg atcctgaact aaggttttct
     5281 gatacttgaa taccgtttta ttcggtttcg ccaaaggaga atgattgatg aa
//
LOCUS       DAJVOY010000221         5289 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJVOY010000221 DAJVOY010000000
VERSION     DAJVOY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.223"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1065
                     /locus_tag="OQL90_003767"
     CDS             <1..1065
                     /locus_tag="OQL90_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCS1370384.1"
                     /translation="RQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLR
                     YGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLV
                     YHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQW
                     YEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFH
                     GVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPH
                     LLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVA
                     MEYAFLVALAQGTLPATPAD"
     gene            complement(1062..1724)
                     /gene="exoX"
                     /locus_tag="OQL90_003768"
     CDS             complement(1062..1724)
                     /gene="exoX"
                     /locus_tag="OQL90_003768"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1370385.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1748..2404)
                     /locus_tag="OQL90_003769"
     CDS             complement(1748..2404)
                     /locus_tag="OQL90_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCS1370386.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(2506..2736)
                     /gene="holE"
                     /locus_tag="OQL90_003770"
     CDS             complement(2506..2736)
                     /gene="holE"
                     /locus_tag="OQL90_003770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1370387.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            2875..3249
                     /gene="yobA"
                     /locus_tag="OQL90_003771"
     CDS             2875..3249
                     /gene="yobA"
                     /locus_tag="OQL90_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1370388.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3253..4125
                     /gene="copD"
                     /locus_tag="OQL90_003772"
                     /pseudo
     CDS             3253..4125
                     /gene="copD"
                     /locus_tag="OQL90_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            4138..4479
                     /locus_tag="OQL90_003773"
     CDS             4138..4479
                     /locus_tag="OQL90_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1370389.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(4861..>5289)
                     /locus_tag="OQL90_003774"
     CDS             complement(4861..>5289)
                     /locus_tag="OQL90_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1370390.1"
                     /translation="TNMRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVE
                     QCRMVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEVRSL
                     AGRLYKETCGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         1313 a         1259 c         1318 g         1399 t
ORIGIN      
        1 cgtcagcgcg ggttaaccag tttgcgccaa attaaccgca agacccggga agtcattggt
       61 attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga acctgaaacc
      121 gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt tgaactggat
      181 atggataccg gtgagcgtcg tgtattaaaa caaacggaag ttcctggttt tgatgcggcg
      241 aattaccgca gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg
      301 ttggtctacc atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt gtatggctat
      361 ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag tttgttagat
      421 cgtggctttg tctacgccat tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg
      481 tacgaagacg gaaaatttct gaagaagaaa aatacgttta atgattatct tgatgcctgc
      541 gatgcattgt taaaactggg ctatggctct ccttcgcttt gttatgcgat gggcgggagt
      601 gcggggggca tgttgatggg cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt
      661 atcgcccagg taccgtttgt tgatgttgta acaacgatgc ttgatgaatc aattcctctt
      721 accactggtg agtttgaaga gtggggtaac ccgcaggatc cgcaatatta cgagtacatg
      781 aaaagctaca gcccgtatga caacgtcacc gcacaggctt atccgcattt actggtaacg
      841 accggtttac acgattctca ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg
      901 cgcgagctga aaaccgataa ccatctttta ttgctctgta ccgacatgga ctcaggccat
      961 ggcggtaaat ctggccgctt taaatcgtac gaaggcgtag cgatggaata tgcttttctg
     1021 gtcgcgctgg cgcagggaac attacccgct acgcctgcgg attaagtatt ttccagataa
     1081 tgtttcagtg ttaaacgcag ctccgggctc atgctgtcca ggttattaaa tgaccagcgc
     1141 agatagcccg gatcgcgttc ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag
     1201 gtgaaggtcg tcatcaacga cggacgtccg gtgatatcgg ccatctgttc tgccgtccag
     1261 ccggaggtgt tcataatatc gataagcaac gcggcggtga tataacagtc atacaacgcg
     1321 cggtgatgat gcaggcccgg cggcgtctgt acattgagct tccgtgtttt atataacgcc
     1381 atattgctgt acttgatccc tggccacaaa cgacgggcca gtttcatagt gcaaatccac
     1441 tcaccgggca tctcaggcag tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat
     1501 tcactaccgt aatagtgtgg gatcacatct tcaatccacg gtttatcggc gaccatggct
     1561 tcggtgatgc gatgaatcgc catcgcttgt ggactaatag gacgatcggg gcgcaccagg
     1621 tggctcatgg ggttgacgat ttttccgtca atgacatcaa cagaggcaat ctcaacgatc
     1681 cctccctgca aaccgcaggt ttctgtatcg ataatgcgca acatggcatg ctccaggccg
     1741 aaaaagccta gcgtaatgga atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga
     1801 ctaacaataa tgaaccacga tcggcgcgaa cgatgagacg accatattca tcccatagtg
     1861 cactgttacc acgggcattt gccatcagca cggcaatcga gtactgatgt gaaaaaaatt
     1921 gtaagcgacg ggccgacgcc agcaggtcgg gttctcccag acattgccct gtagtaaaca
     1981 gtgaacaggt cgggtccata tccatacctt gcggttgttc atcgaccacc gtgatggttc
     2041 tggaacgacg gcccagacat gcaccatgac tctgatggta gatcccaggc gtttttcgcc
     2101 acggtgcgaa caccgcaatt ccacgaataa agcgatcgtt atattcaacg ggaaggccgg
     2161 caatgatggt cattcgccag gtcgttgcgg cataacaaag cgggtctaac agtgagagat
     2221 cgggtggggc aggaagggca cgtcgcgaat aatcacacgc cagtaaagaa agcgatggaa
     2281 aaactaacag ctgacattgt tgcctggcgg cggccctcac aaactccaga tgatgagcta
     2341 cctgttcggt gagtgaggtt ttacgaggct catattgcgc tgctgcaact ttccagaacg
     2401 acataatgac ttccttttca aaatgactgc aaattatggg tgcggtttag aaaatactca
     2461 taatttagca ggcgttatgt aagaaagtgt aactctgtat aagttttatt taagtttggg
     2521 ctcgtaaggt aaacgtgaca gtttgaccga agccaaacgg tgggcaataa gcctctcgcg
     2581 aaaccagctg cgcaaatgtt caggctgttc acgttcaacc gcttcagcga tcaccggcat
     2641 attgtagcgt tctttaaatg ccaccccggc cgccgccaaa tcgacattca ctttatccat
     2701 ttctgtttga tccagtttag ccagattctt cagcattctt aatctcctca tcattcgcgg
     2761 gcaaaagtta ctaagagtga gccaggatgg caagccggaa ttgatgatcc tcgactcaga
     2821 cattagacaa ggttatgctc tgtaaaaaag ttattacaaa aagtaataga taatatggct
     2881 tcaactgcac gctcccttcg ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct
     2941 tccgtctggg cacatgcgca tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca
     3001 gctgcaccgc aggcaatcac tttaaacttc tcggaaggtg ttgaaacagg attcagtggt
     3061 gcaaaaatca cggggccaaa aaacgaaaat attaaaacat taccggcgaa gcgaaatgag
     3121 caggaccaaa agcaactgat tgttccactg gccgattcgc tgaaacccgg aacctacacc
     3181 gttgactggc atgttgtttc ggtggacggg cataaaacga aaggacacta cacctttagc
     3241 gtgaaataaa tcatgctggc gtttacctag atcgcgctgc gatttatcca tttcacctcg
     3301 ctgatgctgg tttttggctt cgcaatgtac ggtgcctggc tggccccctt gacgattcgt
     3361 cgcttgctgg cgaagcgttt tctgcgttta caacagcacg ctgccgtctg gagtttgatc
     3421 agtgccactg caatgctcgc cgttcagggc gggttgatgg gaacgggatg gacagatgta
     3481 ttttcgccaa acatttggca ggctgttttg caaacgcagt ttggtggcgt ctggttatgg
     3541 caaattgttc tcgccctcgt aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca
     3601 cgtttgctgt ttatgctcac taccgcgcaa tttattctgc tggcgggagt ggggcatgcg
     3661 acgctgaatg aaggggtaac ggcgaaaatc catcagacta atcacacgat tcacctgatt
     3721 tgtgcggcgg cgtggtttgg gggattactg ccggtgctct ggtgtatgca actcatcaaa
     3781 ggccgttggc gacaccaggc tattcaggcg ctgatgcgtt tttcatggtg cgggcatttt
     3841 gcggtgatcg gcgtactggc aagcggcgtg cttagtgcat tgttgattac cggatttccc
     3901 cccacgctta cgacttactg gggccaactc ttattgctca aagccatcct ggtgatgatt
     3961 atggtggtca ttgcgctggc taatcgttat gttcttgtac cgcgcatgag gcaggatgaa
     4021 gatcgtgcgg caccctggtt tgtgtggatg actaaactcg aatgggcgat aggtgcggta
     4081 gtgctggtga tcatcagcct gcttgcgact ctcgaacctt tttgatggat tacgaaaatg
     4141 atgaaaaaaa gtattctggc gtttctgtta ctcaccagtt ctgcagcggc gctggctgca
     4201 ccgcaggtga ttaccgtcag tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc
     4261 gaagaggtga tgctgacttg ccgaccgggt aatgctttgt atgttattaa tccgagtacc
     4321 ctcgtgcagt atcctttaaa cgatatcgca caaaaggaag ttgccagtgg gaagaccaat
     4381 gcccaaccca tttcggtgat tcagattgat gatcctaaca atcccggcga aaaaatgagt
     4441 ctggcaccgt ttatagaacg agctgaaaaa ctctgttaat tacctaaaat agccttttga
     4501 tttccaataa aaaaaccgcc tcagttcttt caccagaacg ggcggtttta acatttcagc
     4561 tgatgaccac cacgcttttt attgaccatt ttgcacgcaa actggaaaac ctggcgtcgt
     4621 catctattct taaagggcaa ggcaactaag cctgcattaa tgccaacttt tagcgcacgg
     4681 ctctctccca agagccattt ccctggaccg aatacaggaa tcgtattcgg tcttttttta
     4741 attgcatttg aaatcaatta gttgcaaatg tctccccgaa atttactcga atttctgtat
     4801 tccggtcttt tttggttata tcacaaccaa aatacattta acaatccatt tacgttaaaa
     4861 tcagagcagt aagtacgttt tttctctctc atcaagatac atttttgttg tcttctccga
     4921 tgtgtggcca agtagacgct gagcaaattc ttctccacat gtttctttgt acaatcgtcc
     4981 agccaggctt ctgacttcgt gaaaagttgg tgggttttca ctgaactgga tacctgttaa
     5041 ttttctggct gcgacaaatt tctttgtcag gccgtccggg tgaatgctgc cgtcagggct
     5101 gttttttcta atcccggcac tgattagata atccccccta cttaccatgc ggcactgttc
     5161 aactactgca ccaagtcgta aaccagcgac aggaaggctg agtgacaggg ggatagcaat
     5221 catcattcct gtcttaattt gcctgatgtg gagacgatca tcataaatat cactgaaccg
     5281 catattcgt
//
LOCUS       DAJVOY010000222         5210 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVOY010000222 DAJVOY010000000
VERSION     DAJVOY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.224"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..119
                     /locus_tag="OQL90_003775"
     CDS             <1..119
                     /locus_tag="OQL90_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCS1370391.1"
                     /translation="DWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(169..1917)
                     /locus_tag="OQL90_003776"
     CDS             complement(169..1917)
                     /locus_tag="OQL90_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCS1370392.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLVLLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFLITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1917..2987)
                     /locus_tag="OQL90_003777"
     CDS             complement(1917..2987)
                     /locus_tag="OQL90_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1370393.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2977..4428)
                     /locus_tag="OQL90_003778"
     CDS             complement(2977..4428)
                     /locus_tag="OQL90_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCS1370394.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4439..4885)
                     /locus_tag="OQL90_003779"
     CDS             complement(4439..4885)
                     /locus_tag="OQL90_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS1370395.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1318 a         1344 c         1316 g         1232 t
ORIGIN      
        1 ctgactggga taaagagcaa cttcaggacg caaactcaag ctggatggtc gaagactctt
       61 tcccgagagc attacgcgag tacaacgaaa tggttgatga ctataatagc ctgcgttaaa
      121 cgtttttgat gataaatgca gtgtcggatg cggcgcatct gacacagttc accccttgtg
      181 aagtaactcc ataaatgcct catatcctgt taacccggca ataacctctg ccggatgctg
      241 acacaatatg cttgccagat agctaaatat ttttagctca tgacgcgcat cggcggcggc
      301 gcaggcgatc aagacatggt taatgacttc gttattcacc tcaactggtt gggcgagggt
      361 aataagaaat ccacgaaaac gtcgctcctg ttcgctccag caatgtggga tggcgaggcg
      421 attaacaatc aggttttcac cttcgccttc gcgggcgatg atgcgttgtg cttcatcggc
      481 ggtaatatgg tgttgtgcga ctaattgcgc acaaatttgc cgggtaatat gttgccagct
      541 ttcaccgcgt acattcgaat aatgaaaact tccgggggca gagaaaaaac gctccggttg
      601 ctggcgaata aatgccgtcg ccagaaaatg ttttatttgt tcgataccgg cagccgtaat
      661 gatatttttt acggtgatgt aattatttac cgcgtcttcc agtaaataat ggctattgtt
      721 aatgatcaat aacggctcaa tctcttcgcg aatggcaaca agttcgctta agctacgggc
      781 aataattacc cgacaattta aaacatcgcg ctcaatggcg agctgattaa tagtggcaat
      841 cgcgttctgg tccgagagca aaatgatcgg ctggcgttcg ttttgatgcc gctccagcgc
      901 acaggcaaaa tacaaaccga tcagatcgct gtcgaaaagg ggaatatcga gctgttcacg
      961 cagtagcgta ataaagtgca gactcatatc aaaggccgca ggccaggctg ctttcaggtt
     1021 attcatgcta ctttggcggt gctcagcaat ccacaccggt gcggcaaggc aacgtaataa
     1081 atgcccggta atattctcaa ccaactgcgt atcaataata ttcaatgacg cgatgtctgg
     1141 caccctttgc agcaacccca gcacatgatc ggctgaaatt acctgcgctt gctgatgctg
     1201 tttctctatc aaacctgtca gggttcttac agcgttatca ccaagaaaca tgccgctttg
     1261 ttcaacgatt tgttttatct cgtcaccggg atattgcggc cactcatcgg tcagttgatt
     1321 acgcagggcg taaatcgcca gaatcaggct ggagagataa tcaccctgca tgagcggcca
     1381 gcggtgttgg ttgtcgcagg ccgtggataa atgctgaagg ttgtctcgtg ttacgcccgc
     1441 cagcggaatt aaaaacggat ctttgcgcag caagttcgcc agcaagataa cgcgcttctc
     1501 ttctgtctca tcaatgaaat ggcccaaacc ggggcggctg gccaggcaac aagcgcgttc
     1561 ataacgctgt tttaaccggg gcagacgctc tgcgacccac gtttctggca aattaagcgc
     1621 cgaggcgagt tgcgcacggg gagtaaaggt attcagtaat aacagtacca gcagccgata
     1681 gtcgttatcg tgcttttgca gcaactgaaa aaagctgcgg cgctcgaaga tttccagctg
     1741 atagcccgca ctaccactgg cggaaatgcg ggctttgccg ttaagggtaa agttgagata
     1801 gtcaatatca cgcaggatgg tcctgcctga aacgccagtc tgttgcgcca gctcaccagg
     1861 cgtgcgcggc tgttgctcca gcagatcgac aatctttaac tggcgttcgt ttagcattaa
     1921 tccatctgac ggaactggct aaacgcgttg actttctccg cggttaaggt ggtcagaaaa
     1981 tcccgtatca acgtgagcag agcatcgtaa tcggcttttg aaatcatacc gctgttagcg
     2041 tgcagatagc gggttggcag gcacagcgca acaaccggac gcccgccgcc catcacgttg
     2101 tagcgcccgc catccgtcgc accggttttc atggtggaaa attgcagcgg taaatcgtta
     2161 tgtgcggcac agctttttaa cgctgctacc agtttctggt tggggaagta gcgcttgtca
     2221 aacagcatca gccccggccc ctggcccagt ttcagcgggt atttaatgtt atcaatgccc
     2281 ggaacatcgc ctgcgacagc ggtatccagc acgatcacta catccggttt aatgtgttcc
     2341 gccgaggttt gtgccccgcg tagcccaact tcttcttcca cgctgccgac gccatacagc
     2401 gtaatttcgg gattattcac cgtctgcaat agttcagcca tcattgcgca gccgatgcgg
     2461 ttatccagcg ccttgccgac cactttatct tcgccccagc aggcaaaatt ggcttccggg
     2521 ctaataaaat cgcccatcgc cacaccgcgt ttttccacct catcgcgact gtttgcgcca
     2581 atatcaatga acatctcatc aaatgacagc ggccgttgcc tttgcttttc cgttaacgca
     2641 tgaggcgcga cggaaccaat cacaccaggg attttcactc ccttgtgtgt gcgtatggtt
     2701 acccggtggt tgagcatcga ctgattccac cagccaccaa tggtggtgaa acgcagaaaa
     2761 ccgctctcgt cgatgtgggt gaccataaag ccgacttcat ccatatgccc gacaacggca
     2821 acttttggac ctttattccc cttacgggca acaaagctgc ccagaccatc aaaggtgatc
     2881 tcattaacgc aaggttccag cgtgtttatc agaatgtcgc gaacttcctg ttcatcgccg
     2941 ctgacggcgc tggcttcgca caactgttgc agtaactcaa tgttcatgga caagctcctg
     3001 ttgcgcggct ttcgctttgc ggcgtaacca caatcctttc aggacgataa tgaatgaaat
     3061 gttcagcgcg aggccaatcg ccagcaccag ataaaacgaa cccaccggcg acatcaggcc
     3121 gatcagcggg tcaaagatac ccaaaccagg tgccagacgt ttgattccga aagcgatcac
     3181 cagcacgccg gtaatgccac ccgccagcgt attggcggta atcatcggca acggagctgc
     3241 cagagcgtaa ggtatagccg gttcggttgc gactgtggca ccgaccacaa tggcactact
     3301 ggcagcctct ttttcctgtc gggtaaacag tttgggcgca atgaacgtcg caagacccgc
     3361 cgcaacgggc ggcatcaacg cgacaacgcc gacgatggcg taccagtcat aaatgtgttt
     3421 ttccagcagt gagaagcaga agaaccaggc ggttttattg atggggccgc ccatatcgaa
     3481 cgccagcatc gcaccgacca gaaacgctgc tgcaaacttc attgatggag gaatggtatt
     3541 caggaagtgg agcagcccgc ccatgaggtc ggacatcacc ggcccgataa cgtagtaagt
     3601 gagcacgccg aaaaccagca gagtaacgaa cgggatcagc attgaaccga gcagcggttg
     3661 taatgctttg ccaaggcgca ctttgcggaa ccagaagacg aaatagccaa tcgccagccc
     3721 cagcaccacc gcgccgagga atcccgcacc cgactgggtg ccgagcagcg ctttgtcgtt
     3781 cgccagatag caaaccagaa acgccggagc gaaagccggt ttatcggcaa tcgacgaggc
     3841 aatataagcg cccatgatgg ggatcataaa ggtgaagcca aggtaaccga tggattcaac
     3901 tacccaggta aacgacggcg cgcctttcga catatcggta tacggcaaac cgaactgcac
     3961 cagcatgttg gcaagcgcca ccaggatacc accgccgatg acaaacggca gcgccgcaga
     4021 gacgcccgcc atcaggtggc tcatcacgct gccgctttgc atttcctgtt tgccaagctt
     4081 cacgccacta tctgcggcaa aaagctgcga gtttgtcggt aattcgctga aaatctggtc
     4141 gatatttttc aacgcctggg agatggcaat ctcataaact ttcttcccgg taaaccgcgc
     4201 gcgatcatca ccgctcaggc cacgcccggt agcgagaatg acgtaatcgg cagcggcaat
     4261 ctcttcgctg gataagcggt tttcaacgcc actggaccct tgagtttcca cttttatggt
     4321 atggccgaga gaacgggctt tctgttccag ggcttccgcc accatgtagg tgtgagcgat
     4381 cccggcgggg cagttggtga tcgcgacaat acgtaaggat gactccatcg ccacttcctt
     4441 atgagctgag ggtttgattt aacaaggcaa gcacttggtc ggtatcgccc tgttgcagtt
     4501 gatgaataaa ttccttgtga ataattttgc gacacagtgt gccgatgatt ttgacctgat
     4561 cctcttcgcc gctttgcggc acgccgaggc agatccagca attgacgtct tcgccatcgc
     4621 tggctttcca gtcaatagcc tgcgctttgc gggcgaataa tacgaacggt tgtttaacgc
     4681 aggcgctttt accgtgcggc acggcgacac cggaaccaaa tccagtagag tgcatttttt
     4741 cgcgcagcag cagggtttgc aggaactggt gtgagtcggt gataaaaccg ttttgtaacg
     4801 ctattgtcgc caactgcttc agaacggaat aagcgccatt gccctgaata ttcaggtcaa
     4861 tacagcttgc agtaagagct gccattaatc ggactccata aaatatattt taatcaattg
     4921 taatggtgga gcgattaagt gatgagaggc gatatgtcgg taaacaatga caatttgaga
     4981 cagagtgaaa ggtcagattt gcggagtaat gcacataatg gttatttaaa taaaccacat
     5041 gaatcattaa tgggttattc attatttttg tgatttactc acaatgtatc agggaaatat
     5101 aacttaccgg gagatgtaat atgtttaatt tttcaaatcg aaatttaaaa tattgtgccg
     5161 gaggcatctc cggcacattg ggcaattacg gcaggtaaaa cacttcttgc
//
LOCUS       DAJVOY010000223         5198 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVOY010000223 DAJVOY010000000
VERSION     DAJVOY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.225"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..684
                     /gene="adeQ"
                     /locus_tag="OQL90_003780"
                     /gene_synonym="yicO"
     CDS             <1..684
                     /gene="adeQ"
                     /locus_tag="OQL90_003780"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1370396.1"
                     /translation="CGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSF
                     MLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIE
                     STSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARV
                     NWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAAL
                     FALKIILVD"
     gene            737..1189
                     /locus_tag="OQL90_003781"
     CDS             737..1189
                     /locus_tag="OQL90_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1370397.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1400..2590
                     /gene="nepI"
                     /locus_tag="OQL90_003782"
     CDS             1400..2590
                     /gene="nepI"
                     /locus_tag="OQL90_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCS1370398.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRCYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2631..2924)
                     /locus_tag="OQL90_003783"
     CDS             complement(2631..2924)
                     /locus_tag="OQL90_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370399.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3146..3964
                     /gene="nlpA"
                     /locus_tag="OQL90_003784"
     CDS             3146..3964
                     /gene="nlpA"
                     /locus_tag="OQL90_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCS1370400.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(3991..4890)
                     /locus_tag="OQL90_003785"
     CDS             complement(3991..4890)
                     /locus_tag="OQL90_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1370401.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5001..>5198)
                     /locus_tag="OQL90_003786"
     CDS             complement(5001..>5198)
                     /locus_tag="OQL90_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1370402.1"
                     /translation="YFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1246 a         1196 c         1334 g         1422 t
ORIGIN      
        1 tgtggattat ttttcggtga tgttcatttt agcggcgtct attccattcc gcctgatatt
       61 agcggcgtca ttggtgaagt agatttgagc ggcgcgttaa cacttgaact cgccggtatc
      121 attttctcct ttatgctgat caacctattt gattcatcag gaacattaat tggtgtaact
      181 gataaagcgg gcttaataga tggtaacggt aaattcccca atatgaataa ggcgctgtat
      241 gttgatagcg tcagttcggt ggcgggtgcg tttatcggca cctcgtctgt tactgcctat
      301 attgaaagta cttctggtgt ggcagtcggt ggccgcacgg ggctgactgc ggttgtggtt
      361 ggcgttatgt tcctgttggt tatgttcttc tcaccgctgg tggcgatggt tcctccttac
      421 gcaaccgccg gagcgttaat ctttgttggc gtgctgatga cttcgagcct ggcgcgcgtt
      481 aactgggatg attttaccga atcggtgcct gcgtttatta ccacggtgat gatgcccttt
      541 actttctcga tcaccgaagg gattgcactc ggctttatgt cgtactgcat catgaaagtg
      601 tgcaccgggc gctggcgcga tctgaacctg tgtgtggtgg tggtcgcagc tctgtttgca
      661 ctgaagatta ttctggtgga ttagtaactg gcgagggttt ccagatatca tgagttctga
      721 ttacgcagga gaactcatga tctggataat gctcgccacg ctggcggtag tgtttgtggt
      781 tggttttcgg gtgctgacat ccggggccag aaaagcgatt cgccgtctca gtgatcggct
      841 gaatatcgat gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt cagccggtga
      901 cgaattttta cgttatttgc atcgcccgga tgagtcgcac ctgcaaaacg ccgcgcaggt
      961 gttactcatc tggcaaattg tcattgtcga tggtagcgaa cagaacctgc tgcaatggca
     1021 tcggatttta caaaaagccc gtcttgccgc gccgattacc gatgctcaag tcaggctggc
     1081 gctgggtttt ctgcgcgaaa ccgaacctga aatgcaggat attaatgctt ttcagatgcg
     1141 ctataacgcg ttctttcagc ctgccgaggg cgttcactgg ctgcattaaa accgcttcgt
     1201 cttatcagcc tacagaccgc gcctgacctg tcgggtggat aagacgttca cgccgcatcc
     1261 ggtaatcttt gccctgttcc gtttcgatct taactccagc tcaagcaaca ccgtaaaagt
     1321 tgcaaaaacg ttaaatgcat cacacatttc aatgttaaag tggtcggctt ttcccctgaa
     1381 acatgccacg ggtaacacca tgagtgaatt tattgccgaa aaccgcggcg cggatgccat
     1441 cacccgaccg aactggtcag ccgttttctc ggtggcgttt tgtgtcgcct gtctgattat
     1501 cgttgagttt ttgcccgtca gtttgttgac gccaatggcc caggatttag gcatttcgga
     1561 aggggttgcc gggcaatcgg tgaccgtgac cgcctttgtg gcaatgtttg ccagtttgtt
     1621 tattacccag acaattcagg ctactgaccg ctgctacgtt gttattttgt ttgccgtttt
     1681 gctgacgctc tcctgcttgc tggtttcctt tgctaactca ttcagtttgc ttttaatcgg
     1741 tcgtgcctgt ctggggctgg cgctgggcgg gttctgggcg atgtcggcgt cgctgaccat
     1801 gcgtctggtg ccgccgcgta cggtgccgaa ggcgctgtcg gtgatcttcg gcgcggtttc
     1861 tattgcgctg gtgattgccg cgccgttggg cagtttttta ggcgagctta tcggttggcg
     1921 caatgtcttt aatgcggcgg cggtgatggg cgtgctgtgt attttctgga ttatcaaatc
     1981 attgccttca ctgccaggcg aaccctcgca tcagaaacaa aatactttcc gcttattaca
     2041 acgtccgggt gtgatggcag ggatgatcgc catcttcatg tctttcgccg ggcagtttgc
     2101 tttcttcacg tatattcgcc cggtgtatat gaacctggcg ggattcggcg tggatggctt
     2161 aacgctggtg ctgttgagtt ttggtatcgc cagctttatt ggtacgtcgc tttcgtcgtt
     2221 cattcttaaa cgttcggtaa aactggcctt agcaggcgcg ccgttaatac tggctgtgag
     2281 tgcgttggta ctgacgttgt ggggaagcga taaaatcgtt gctaccggcg tggcgattat
     2341 ctgggggcta acttttgcat tggttcccgt cggctggtca acgtggatca cccgctcgct
     2401 ggccgatcag gcagaaaaag ccgggtctat tcaggtggcg gttattcagc ttgctaatac
     2461 ctgtggcgcg gcaatcggcg gttatgcgct ggataatatt ggtctgactt cgccgttgat
     2521 gttgtccggc acattgatgt tgctgactgc attgttggtt actgcaaagg tgaaaatgaa
     2581 gaaatcctga tatcaggtaa agaagcgttg gtcagtagtt gttgctgttc ttacatatcc
     2641 gggcattcta ccttacccaa cgcttcccaa taattgctct gattattacg ttccatcgcc
     2701 acgatggcct cgcgtatatg ttgtttattg ttttcatctg acaacattaa cttttcccac
     2761 gcttcatctg ataatgacgc ctgtggcgtg caaattttac gtaaatcttg taataccgtg
     2821 gttttctctt ttgcggactg cagtgagcta aaaggcgact cggcgtatac gatgcctggc
     2881 atagcgaaaa aagtgaaaat aagcagtagt gtcgttggtt tcatggattc aaccttttac
     2941 aaatgtgtat cgaatgaagc ataagtgtag tttgctttca tcgctctcat cattctgtac
     3001 gaaacgcgct tgcgtggtca gtaagaagtg ccagacttta tattccactt ttattccttt
     3061 ttattcttat cgatagcgtt tcgtttttta aaccgcagcg accttaccgc tatagtcagg
     3121 taatcattaa taaaaggata aaaaaatgaa actgacaaca catcatctac ggacaggggc
     3181 cgcattattg ctggccggaa ttctgctggc aggttgcgac cagagtagca gcgatgcaaa
     3241 acacattaaa gttggcgtta taaatggcgc agaacaagat gtcgcggaag tcgctaaaaa
     3301 ggtggcaaaa gagaaatatg gcctcgatgt tgaactggta ggatttagcg gttcgttgct
     3361 accgaacgat gcgacaaatc atggcgaact tgatgcgaat gtcttccagc atcgaccctt
     3421 tcttgaacaa gataatcagg cgcatggtta taaactggta gcggtgggaa atacgtttgt
     3481 cttccctatg gcgggttatt ccaaaaaaat cacaaccgtg gcgcaaataa aagaaggcgc
     3541 gacagtggcg atccccaacg acccgaccaa ccttggtcgc gcacttttac tgttgcaaaa
     3601 agagaagcta attactctga aagagggaaa agggttatta cataccgcgc tggatataac
     3661 cgataatcct cgtcatttgc agattatgga actcgagggg gcgcagcttc cgcgcgtact
     3721 ggatgatccc aaagttgatg tagcgattat cagcaccact tacattcagc agacagggct
     3781 ttctccggtg cacgacagcg tatttattga agataagaat tcgccgtatg tgaatatttt
     3841 ggtggcacgg gaagataata agaatgcaga aaacgtgaag gaatttctgc aatcttatca
     3901 atcacccgaa gtcgctaaag cggcagaaac catttttaac ggtggcgcgg tgcctggctg
     3961 gtaattctca ctcatgccgc gccggacgat ttatttttct ggccggcgac gggaatccat
     4021 tgaaatcaaa atcaccgatg acagaatcag cagcgttccc agccagtccg gtaatgtgaa
     4081 cgtgatcccc agcaacagca aagagagtag cgcgctactt aatggttctg cacagctcaa
     4141 aatgctggct tttggaccgc caattaattg tgctcctttc aggtacagac taaatgtcag
     4201 ggacgtacca atgaccacca aataaaaaaa cgccagaatc aaactgccgt taacgacaaa
     4261 gtttgttcct tgtctggcat aaaaaggcaa cagaatcaga ccgccaatca gcatactcca
     4321 gccgacgact ggtaacgtgc catagcgggc aattagcgtt gagggatagg tggtatagaa
     4381 tgcagcagca aatgccgagg caatgcccca gaacaacgcg gcaggagaga gcgataatga
     4441 cgtcggatta ccgtgtgtca ccaataaaaa agtcccgacc agcgatgtca aaatagcgca
     4501 gaaaaccaga atgcccgggc gcgatttacg caccagtgag aaccaggcga cgataatcgt
     4561 cggtgagagg aattgcagca ccgttgccgt ggctgcgttc gatttttcga tggttagcaa
     4621 aaaagtgagc tgtacagtta gcgcgccaac cacggaaaaa atcagcaggc taatggcatc
     4681 tttatgattg ttaataatag aaaagatttt atcgccatga acaaatgaca gcgtcagtag
     4741 aatcaaaccg gcgaatatca aacgcgtcat agtcaaaaac tgcgacgaca tctggctttg
     4801 ctccatgatg tattgcgcgc agacccctga acttccccac aacacggcgg caatcagaac
     4861 gttcagcatc ccctttctgg tggaacccat ttttccctct gcatactgtt ttgtcgttta
     4921 tgtatgcagg catgatagca aaaatggcga ggatggctct gtttgcgcca cgccgatttt
     4981 tcttagccca gactggtgag ctaaatatct ttaataaaca gcaggcaaat catcgcaata
     5041 cccaacatcc ctatcgccag ccagaacaac gcatggtagc tccagatttc aaccataatt
     5101 ccgtcaacag agccggcgat aatccagccg acgcgtgaag tatttgcata taacgttgtc
     5161 gcagagccta ttttttcagg cattaagtcc tgaaaata
//
LOCUS       DAJVOY010000224         5108 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVOY010000224 DAJVOY010000000
VERSION     DAJVOY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.226"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..493)
                     /gene="hyfE"
                     /locus_tag="OQL90_003787"
     CDS             complement(<1..493)
                     /gene="hyfE"
                     /locus_tag="OQL90_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1370403.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHL"
     gene            complement(505..1612)
                     /locus_tag="OQL90_003788"
                     /pseudo
     CDS             complement(505..1612)
                     /locus_tag="OQL90_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(1624..1734)
                     /locus_tag="OQL90_003789"
     CDS             complement(1624..1734)
                     /locus_tag="OQL90_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCS1370404.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(1751..2698)
                     /gene="hyfC"
                     /locus_tag="OQL90_003790"
     CDS             complement(1751..2698)
                     /gene="hyfC"
                     /locus_tag="OQL90_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1370405.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(2709..4726)
                     /gene="hyfB"
                     /locus_tag="OQL90_003791"
                     /pseudo
     CDS             complement(2709..4726)
                     /gene="hyfB"
                     /locus_tag="OQL90_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(4726..>5108)
                     /gene="hyfA"
                     /locus_tag="OQL90_003792"
     CDS             complement(4726..>5108)
                     /gene="hyfA"
                     /locus_tag="OQL90_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1370406.1"
                     /translation="SLCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQADGSLKDGE
                     ENAPTQHALLRWEPGVQTVAVKCDLCNFLPEGPACVRACPNQALRLITDDSLQRQMKE
                     KQRLAASWFANGGEDPLSLTQEQH"
BASE COUNT         1392 a         1442 c         1355 g          919 t
ORIGIN      
        1 ccagatggga gccgttttcc atcaggcagt aaccaaaaat ttgccgcagg atattgcgct
       61 ggctgacaat gcacagcagg ccaagcagaa aatgacctaa cgctaccgcc agcgccggtt
      121 tcagcccggt agccatcggt agcttcacgg gctgaacgac aaatgcgcaa agcaggacaa
      181 ttaacgccgc gagcagtgcc atcattgccg gaccgaataa cgctttttcc gggatgttct
      241 ggggaatatt tcgtgcagcg taagtcatga ttaacggtac cagcagcact ttggtgataa
      301 aggcgctggc ggaccagatc agcagttgct ctgcggcgaa caggcacgag agagtggcga
      361 aaatagacac cagcaccagc gactggcagg cgtaaaatcc gcatgacagg cgatagcttt
      421 tgacgctaat cacaaacagc gatgtcagca tcatcagtcc cgccagatta tttacgatca
      481 tagaaccgat cattcatcat ctccttactg caaccaggtc gcggcgatta cgctggaaat
      541 cagcgacatc acaatcagta caatcaacac caggcgcatt gaccctggca gcggtgcggc
      601 atcggcgacg gcctcgctcg gtttgccagg cacaacgcga ccaaaccagc gaataaacca
      661 ggcgaaactg gcgaccgatt caatcatcag cagaatcatg gcgggcagca ggatccagta
      721 ctccactgac aacgcaaaac cggcggcaaa cagcgggaat ttactgaaga agccgttgaa
      781 cggcggcaca ccggtaatcg ccagcgctgc cacgcagaaa cccacgcctg gcaacggcag
      841 ggagtgcaat acgccacgca gacgcggcaa caagcgcgtg ccgcagctgt aactcagcgc
      901 acctgctaca aggaaaaaca ggcttttagc gaacgcgtgg ttgacgatgt aggcgatgct
      961 gccctccagt gccagccgcc cactttcacc atcgatgcgg cgtggagata ggctctgatc
     1021 ggtgttggcg cttccattgc gtccggtagc cacgcttgca tcggtagctg ggccgatttc
     1081 ccccacgcgg caaacaggat gccgccataa accagataac gtgcgtcgcc atgtaactcg
     1141 ctcatcgcgc taagcacaaa cgttccggtc tgcaaaaaca gcgtggcggc ggcaagatac
     1201 aaccccaacg aaccgatatg ggtgataagc aacgctttta gtgttgaacg ctgcgcttta
     1261 tcgctctggt aataactgat caacgcccag gagcagccgc cctaatttca aaaaacaaca
     1321 actgaccgag cagcgtcgag gagagtacca gtcccgccat cgcgccgata aacaccagca
     1381 aaaatgcgta ataacgattc gtgccgttat gcgggtgttc gcgattttta tccgtcagat
     1441 aacccgtgga gtagatcgtg accagcaaac cgagaaacac caccacaaac agaatcagcg
     1501 tactcacgcg atcaatgacc aggccaaaca atgccacatc ccccacgttg accaacgtca
     1561 acgtgacggc aactttatcg gcctgataaa acgcggagat cagcgacaac atgcacagcg
     1621 tggtcagtgc ggcgaacaaa acccccattc ggcggcccga cgttgtggcg aaaacgaaac
     1681 gaccagtgcg ccgataaaag gcagcattaa cgtcgtcaga gcaagatttt ccatattccg
     1741 tcagtgctcc ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc
     1801 caagccaggt cacatggtga atgagtaaaa aacgcccgcg tgccagcgtg ttttctgcga
     1861 ttgaggccag tacaaaaatc agcaaaacct tgagcagcgt aacgacaagt gaagtcagca
     1921 ggcaggcgag agaaagttct tgcgcgcgcc caaagggcag aaacagggcc acaaacagtg
     1981 ctgccatcac gacctgtttc agccccagcc cccatttcgc tagcgccagc ccggcaccgg
     2041 aatattcggt cagcgggcct tcctgtaatt cctgttctgc ttcagcaaca tcaaagggaa
     2101 tttttcccat ctcaatgaag caggcaaaac cacaggccag taacgccagt acggtggtta
     2161 gtggcgagtt ccagcccgtt gccagcgtat tgctgatcat ctcgatatgc gtggaacctg
     2221 ctatcagcgc caataccagc agtgagagaa tgagcatggg ttcgaccaga atgccgagcg
     2281 tcaactcgcg actggcaccg actcccgcaa acgggcttcc ggtatccagt ccggaaagag
     2341 caaagaaaaa acgaaacagg gcaagaagat agataagggt gatcagatcg ccgccgcccg
     2401 caaaagggga aacggtaata aaaagtggta gggccatcgc cagcaccagc atgctgctga
     2461 ttaataccca cggcatcagg cggaacatca gacctgaaga tataggcgca acttcctggc
     2521 gtttaaacag tttgtggatc tcgcgataat cctgccagat ccccggcccg cggcgggagt
     2581 gcatacgcgc gcgtatctgc cgggaaatac ccgtaaaaag cggggttagc agcagcagaa
     2641 tcacggcctg tgctaacgca aaaatgacca gatatccgtc gcaaagagtt tgtctcatgg
     2701 tgatttcctt agacggcaat agcgattagc agcacaacca gtgcggcaac tagatacagg
     2761 caatagagac gaaagtcgcc gctttgcaga tgctgtattt ctttggccag ccgttgggtg
     2821 gcgctcacga tggggcggat cacccgctca tcccagaagg gttctgtgct ctgagccctg
     2881 gcggtgacgt gcgcaagatc tttatttagc ctcagcgtag ggtcgagctg ttgtcgtaga
     2941 cgaaataacg cagagaagac cacacgcagc ggctgcatca cgccattgcc tgacggagcc
     3001 atcgcgtttt cccagccgta gccgcatgcc caggcatctc ctgtgcgacg aaacgctgcg
     3061 cggcgcgaac gacaccacag ccagtaaagg ccaggcagca aaggcatcgc cagtagtaac
     3121 agcaacagta atgaaggagt gacccgtgta tgaaacgtgc cgggtacaag tgctattccg
     3181 ctggcgacag tgacgggagg ggtattggta aacgcatggg caatatgcat tatcttcggt
     3241 gccagccaac tggcactaag cgcaattaat acgcagagtg ccgcgagcag tagcatggcg
     3301 acgatcatcg tatttggcac ttcctgtgcc tcttcagcgt gtgtactgcg cggcgcacca
     3361 cagaaagtaa taccgtacat tttgacgaag cacattactg ccagcccacc agtgactgcc
     3421 agcattacca tagcaatagg acccgcaagt tgtagcgcta cggcttccac acgacttagt
     3481 gagaacagcg actgccaggt gtaccattcg ctgataaaac cattcagcgg aggaatggct
     3541 gatatcgcga ggcaaccaat caggcatgct gcggctgtcc acggcatccg tttcgccagt
     3601 gcccccattt tttccatgtc gtgggtatgc aaacgcgaaa taatcgcgcc cgcgccgaga
     3661 aatagcagcc ctttgaacag cgcatggttg agcagatgaa acagtgcgcc gagcagtcca
     3721 accacggtga gcagcgggtc gtgcagtgac agaccgtcca tcgccacacc gactgccagc
     3781 aaaataatgc cgacgttttc gacggtactc caggccagca gccgtttgat atcctgttcc
     3841 gccagcgcat atagcacgcc caggagcgcg gagattgcgc cgatcgccat caccagaatg
     3901 ccccaccaca gaggcaaacc cgtttgcgcc agcagatcca tcgctacttt caggatgccg
     3961 aaaataccga ttttgaccat tacgccagac atcaacgccg aagcgtgcga tggtgcggca
     4021 gggtgagcgc gcggcaacca gctgtgcaac ggcatcatcc cggctttcgc gccaaaaccg
     4081 aaaaaggcca gcagaaacat cgccgacgcc agccccggag aaagtgaaag cgtgcgaaac
     4141 tggcaaaatc gaggctgccg ctttcgcgcc ccatcagcaa gaaggcgatc ataatcagca
     4201 ccgatccggc gtgggcgata aaaaagtaga gcatgccagc gttgatcgac gttttatcct
     4261 gcctggcaat gaccagaaac caggaagaca gcgacatcat ttcaaacagc acgatgaacc
     4321 aaaaagcgtt gtccatcacc agcagggcaa ccatcgatgc gatgaaaaga ttcataaaga
     4381 agcccatcgc cgccgcgcct ttgccctcgt attcgcgcat ataagtcaat gaatagagcg
     4441 aacaaaccac caccagcaag gagataacca gcaccataaa taccgagagg ctatccattc
     4501 gcagggttaa atcggcaaag tcaaaagggg tggcaaaacg ggcgactaaa gttacaccag
     4561 catgtaattg cgtaattccg ctgatgatgc caatcacgcc gcccaccagc gatgtgatgc
     4621 cggaaagctt aatagccagt ctgtccagac cgagtaaaaa cagcgaagcc agactggcaa
     4681 acagaaagag gatcagcgac caggttaata attgcagggc atccattagt gttgctcctg
     4741 agtgagggaa aggggatcct ccccgccatt ggcaaaccag cttgcggcaa ggcgctgttt
     4801 ttctttcatc tgacgttgca ggctatcgtc ggtaatcagc cgtaacgcct gattcgggca
     4861 agcgcgaacg caggccggac cttctggcaa gaaattacac aggtcgcatt tcaccgcgac
     4921 ggtctggaca cccggttccc agcgcagcaa agcatgttgg gtcggcgcgt tttcttcgcc
     4981 atctttgagt gagccgtcag cctgaaaaac atattgcgca ggggcattca ccggacggct
     5041 tcctgaagcg ctgattgcgc caaatgggca gaccacggcg caaagcttgc agccaataca
     5101 gaggcttt
//
LOCUS       DAJVOY010000225         5025 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVOY010000225 DAJVOY010000000
VERSION     DAJVOY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.227"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(4..79)
                     /locus_tag="OQL90_003793"
     tRNA            complement(4..79)
                     /locus_tag="OQL90_003793"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Val,seq:tac)
     gene            complement(126..201)
                     /locus_tag="OQL90_003794"
     tRNA            complement(126..201)
                     /locus_tag="OQL90_003794"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Val,seq:tac)
     gene            complement(246..321)
                     /locus_tag="OQL90_003795"
     tRNA            complement(246..321)
                     /locus_tag="OQL90_003795"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Val,seq:tac)
     gene            581..1996
                     /gene="gltX"
                     /locus_tag="OQL90_003796"
     CDS             581..1996
                     /gene="gltX"
                     /locus_tag="OQL90_003796"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1370407.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            complement(2047..2439)
                     /locus_tag="OQL90_003797"
     CDS             complement(2047..2439)
                     /locus_tag="OQL90_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1370408.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2441..2800)
                     /locus_tag="OQL90_003798"
     CDS             complement(2441..2800)
                     /locus_tag="OQL90_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1370409.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3021..3096
                     /locus_tag="OQL90_003799"
     tRNA            3021..3096
                     /locus_tag="OQL90_003799"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3054..3056,aa:Ala,seq:ggc)
     gene            3136..3211
                     /locus_tag="OQL90_003800"
     tRNA            3136..3211
                     /locus_tag="OQL90_003800"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3169..3171,aa:Ala,seq:ggc)
     gene            3420..>5025
                     /locus_tag="OQL90_003801"
     CDS             3420..>5025
                     /locus_tag="OQL90_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCS1370410.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTP"
BASE COUNT         1157 a         1226 c         1285 g         1357 t
ORIGIN      
        1 aagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc
       61 ccagctgagc taatcaccca cttcggtact tcacattgtt acaagaaatt tagctggtga
      121 gaaagtggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc
      181 tcccagctga gctaatcacc cacttctcaa tttcttgcta cacggcggag actacataaa
      241 gtagttggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc
      301 tcccagctga gctaatcacc ccccgctgtg tggagtcgca ttatagggag agttcaaaat
      361 gagtcaacgc attttctaaa gaaattgttc gttcgtcgta aatttaagca agatgatcgc
      421 aaaatagacc gtgttgcgca atttgtcaac gaaaacaata atgcgtaagg tagaaacccg
      481 aactacattg aggaatcagg cgggagtgat agaatatcgc ccactcaatt tttccaggat
      541 ttgccggttg tcggcatctt tctaaacgta aggccatttc atgaaaatca aaactcgctt
      601 cgcgccaagc ccaacaggct atctgcacgt tggcggcgcg cgtactgccc tttactcctg
      661 gctttttgca cgtaaccacg gcggtgagtt cgtgctgcgt attgaagaca ccgatcttga
      721 gcgttccacg ccggaagcta tcgaagccat tatggatggc atgaactggc tgagcctgga
      781 gtgggatgaa ggtccgtact accagaccaa acgttttgat cgctacaacg cggtgatcga
      841 tcagatgctg gaagagggca ctgcttataa atgctattgc tctaaagagc gcctggaagc
      901 gctgcgcgaa gagcaaatgg cgaaaggtga gaagccgcgt tatgacggtc gctgccgcca
      961 cagccatgag catcacgctg atgatgaacc gtgcgttgta cgttttgcta acccgcagga
     1021 aggttctgtt gtttttgacg atcagatccg tggtccgatc gagttcagca accaggaact
     1081 ggacgatctt attattcgcc gtaccgatgg ttccccaacc tataacttct gtgtggttgt
     1141 cgatgactgg gatatggaaa tcacccacgt tatccgtggc gaagaccata tcaacaacac
     1201 gccacgccag atcaacattc ttaaggccct gaaagcgccg gtgccggttt acgcgcacgt
     1261 ttctatgatc aacggcgatg acggtaaaaa actgtccaaa cgtcacgggg cggtcagcgt
     1321 aatgcagtat cgtgatgacg gttatttgcc agaagcactg ctgaactatc tggtgcgtct
     1381 gggctggtcc cacggcgatc aggaaatctt cactcgtgaa gagatgatca aatacttcac
     1441 cctgaatgcc gtcagcaaat ctgccagtgc gttcaacacc gacaagctgc tgtggctgaa
     1501 ccatcactac attaacgcgc tgccgccgga gtatgttgct actcacttac agtggcacat
     1561 tgagcaggaa aatatcgata cccgtaacgg cccgcagctg gctgatctgg tgaaactgct
     1621 gggcgaacgc tgcaagacgc tgaaagagat ggcacagagc tgccgttatt tctacgaaga
     1681 ttttgctgag ttcgatgccg acgccgcgaa aaaacatctg cgtccggtag cgcgtcagcc
     1741 gctggaagtg gttcgtgaca aactggccgc gattactgac tggaccgctg aaaacgttca
     1801 tcacgctatt caggcgacgg cggatgagct ggaagtgggt atgggtaaag ttggtatgcc
     1861 gctgcgtgtc gccgtaaccg gtgcggggca gtctccggca ctggatgtta ccgttcacgc
     1921 aattggtagg acccgcagta ttgagcgtat caacaaagcg ctggctttta ttgctgaacg
     1981 cgaaaaccag cagtaattag cgcataaaag ataaacggca ggagataata tcctgccgtt
     2041 ttttatttat gctgattcgt caatatcgag acgggcgagg aaattgcgaa ttccttcacg
     2101 tgacaaaaat tgcgcgactt tttcctgctc aatgtctgtc atattatcta tcgcgttgat
     2161 gatttgtcga tacgccgggg cgcgtactcc aggagcgtat tcagcaattc tctcttcagc
     2221 ttccatcaac attggtgtgt tatggaaggc tggcgtgttc tgaataaact cgcaaacttg
     2281 cgtatcgacc agaataagtc tggcacgacc gcctttcacg ccgggaaatt tttccgtttt
     2341 ccagcctttt tctctgatcc aacggttaac agtttgttta gccacaccga ggcattcagc
     2401 cagttcttcg gtggtcattt tattgcgtaa tcttttcata ttatttgcta tcgcgaatcc
     2461 ctaagcgttg taacaatccg ataatccctt cccgcagaag caaggatgta aactgttttt
     2521 gttcaactgg cgtcatctct ttcgccagtg tcaccagcaa tacttcaagc ggcgcatcac
     2581 cagaaagggc aggcgcttct ccctgacctt ctggacgttc agcattgcga atatattcac
     2641 gaacctgttc attgacgtga accagtcggg ctttgccacc ctggacgcca ggttttggtg
     2701 acgttgtcca gccttccttg cgtacccatt tattaatggt ctggcggcta tagccggtca
     2761 gtctggcaag ttcatctggt gtcatcctct ccttgaacat aacaattccc tgaatgtatg
     2821 tgggtctatt agtagttcat tttataacac cactttacag gaaaccgtga catgaataac
     2881 tactgaatgc gagcggtttt ccaaaattgt tcatttgcct gatttttcag cgattgaaac
     2941 tacacgttgc aatttgccgt tgacacattc gggcggaatt catatgatgc cgcccgtcaa
     3001 ctcgacaagc tttacgtgac ggggctatag ctcagctggg agagcgcttg catggcatgc
     3061 aagaggtcag cggttcgatc ccgcttagct ccaccaaatt ttgcacccag caaatttggt
     3121 acgtaaacgc atcgtggggc tatagctcag ctgggagagc gcttgcatgg catgcaagag
     3181 gtcagcggtt cgatcccgct tagctccacc aaatttccaa ccctcgctgc aaagcggggg
     3241 ttttttgtct ctgctttttg ccgcttttgt aatacagtct acgtccgggt tagtgccgcc
     3301 tggtgaaagc atcattggat gaaaaatcgg caacaggctg gccccctgtt tgcttcgcga
     3361 tgcgaataaa cttattattt gtgtgcctga aaactccgat cagtgagagt agtgtactca
     3421 tgtttgtgga gcataacctg ataaaaaata tcaagatatt cacactagcg tttacgctca
     3481 ccgtggtact tattcagcta tcccgtttta tttcgccact tgccattatc cattccagtt
     3541 atatctttct ggcgtggatg ccactgtgcg taatgctgtc aatcttgttt atctttggct
     3601 ggcgaggtgt cgttcccgtt ttatgcggga tgttttgcac caatctgtgg aactttcatc
     3661 tctctttttt acagactgcg gtcatgctcg gtagccagac gtttgtcgtg ttgtgtgcct
     3721 gcgcaatatt acgctggcag ctggggacgc gttggcgtta tggattgacc agccgatatg
     3781 tctggcaacg tctgttctgg cttggtttgg tgacgccgat cggcatcaaa tgcagcatgt
     3841 atcttgtggg gagcttcttt gatttcccgc taaagatatc cacctttttc ggcgatgcgg
     3901 atgccatttt cacggtcgtt gatttgctaa gccttttcac cgctgtgctg atttacaaca
     3961 tgcttttcta ctatctcact cgcatgattg taagtcccca ctttgcgcag atattgtggc
     4021 gcagggatat cgcgccgtcg ttgggcaaag agaaacgcgc atttacctta agctggctgg
     4081 cagcgttaag cgtgcttcta cttctgttgt gcacacctta tgaaaacgac tttattgccg
     4141 gttacctggt acccgttttc ttcatcatct ttaccctcgg ggtcggtaag cttcgctatc
     4201 cgtttttaaa tctcacctgg gctgtttcaa cgctttgcct tctgaattac aaccagaact
     4261 ttttgcaagg ggtggaaacc gaatattcgc tggcatttat tcttgcggtg ctgatttcct
     4321 ttagcgtttg cctgctctat atggtgcgca tttatcatcg cagtgaatgg cttaatcgcc
     4381 gctggcattt gcaggcgctg accgatccgt taacgctcct gcccaacttc cgtgcgttgg
     4441 aacaagcgcc ggagcaagag gcgggcaaga gtttttgctg cctgcgcatt gataatcttg
     4501 agtttatgag tcgtcattac ggcttaatga tgcgcgttca ctgtatccgc tcaatttgcc
     4561 gtacgctgct gccgttgatg caggaaaacg aaaagttgta tcaattgccg ggtagtgaac
     4621 tgctgttagt gctgagcggg ccggaaacgg aagggcgact ccagcatatg gttaacatcc
     4681 tgaatagtcg gcaaattcac tggaacaata ccgggctgga tatgggctat ggtgctgcct
     4741 gggggcgttt tgatggaaat caggaaaccc tgcaaccctt gttggggcag ttaagctggc
     4801 tggcggagca atcctgcgca catcatcatg tgctggcgct ggatagcaga gaggagatgg
     4861 tttccgggca gaccactaaa caggtgctat tgctgaatac cattcgcacg gcgttagatc
     4921 agggtgattt gctgctctac gcccagccaa ttcgcaacaa agagggtgaa ggttatgatg
     4981 agatcctcgc gcgactgaaa tatgacggcg gcattatgac cccgg
//
LOCUS       DAJVOY010000226         5016 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVOY010000226 DAJVOY010000000
VERSION     DAJVOY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.228"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            148..264
                     /locus_tag="OQL90_003802"
     CDS             148..264
                     /locus_tag="OQL90_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1370411.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            628..1470
                     /locus_tag="OQL90_003803"
     CDS             628..1470
                     /locus_tag="OQL90_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1370412.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1473..2561
                     /gene="capU"
                     /locus_tag="OQL90_003804"
     CDS             1473..2561
                     /gene="capU"
                     /locus_tag="OQL90_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1370413.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2566..3516
                     /gene="virK"
                     /locus_tag="OQL90_003805"
     CDS             2566..3516
                     /gene="virK"
                     /locus_tag="OQL90_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCS1370414.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQDLN"
     gene            3581..4525
                     /gene="lpxM"
                     /locus_tag="OQL90_003806"
                     /gene_synonym="msbB"
     CDS             3581..4525
                     /gene="lpxM"
                     /locus_tag="OQL90_003806"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1370415.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRYNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(4679..>5016)
                     /locus_tag="OQL90_003807"
     CDS             complement(4679..>5016)
                     /locus_tag="OQL90_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1370416.1"
                     /translation="SKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHAERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT         1411 a          964 c         1156 g         1485 t
ORIGIN      
        1 tatcggtggc aatagcctgt gcaaattttt tataacccgg gatcgtgtca ggattcagag
       61 tatgataaag acagattttc atggctgccg gtttccgtcg tgcaaaaaac atgctgattt
      121 tacgaaggaa tgccttactg aacaaccttg cccggaatac cccccgaaat cagatggaac
      181 aatcagaaaa atgctctgca gttatggaat aaagaaggtg gggtggtacg ccgggagggc
      241 gatccgctgg aaatggcgca gtagctgcag gcctgtcatg atgccggaat agaagttcgt
      301 gtacaggtta atgaaagcgt caccccgtaa ataaactctg cgctgaaatt cctttttttc
      361 attggagcac ctgtgttgtt ctgaggtgaa catatcacct ctgttcaggt agccaaattc
      421 agtgtgccac ctcaaggttc ttagtgagtc gtttttaaaa gatatgataa tatatcattg
      481 tttatgaatt gttgatgttt aatttagtaa aatatatcaa gtatgccatg ggtttgctat
      541 gttattcatc cagtatctgg aaataaaatg caccagcttt attgggtttt ccttttgtct
      601 ctgcttacta ggtgccagaa actttaaatg ttaaacgaag ggggcatttt gttcaaagcc
      661 aatcatgttc cggtattaat gtatcatcat gtcagtcatt gccccggact ggttacactt
      721 tctcccgtga cattccggaa gcaaatgaaa tggttggctg aaaataactg gaaaacactg
      781 tcatcagatg aactggagtt tttttatcgg ggcggaaaac tcccccggaa aagcgttatg
      841 ttgacatttg atgatggata ccttgacaac tggtttcagg tatatccttt attaaaagag
      901 tttaatctca aagcacatat ttttttaatt accggtttta ttgggaatgg tccggttcgt
      961 cactctcctg gaaaggaata ttctcatcgc gattgcgaac atcaaatagc gacagggaat
     1021 gctgataatg ttatgcttcg ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc
     1081 gaatttcatg ttcatacgca tactcataca cgctgggata aaaagttcag ttcacgagaa
     1141 gaacagtgta aacatcttcg gcaggacttg ttgagtggga gagaatattt gaaagaaatg
     1201 acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt attataacaa ggattatatt
     1261 caggtcgctg aagaacttgg attctattat ttatatacaa cagaaagaag aatgaatgct
     1321 cctgctaaag gaactacacg gataggaaga ataagcacca aagaacggga aagttgcgca
     1381 tggctgaaaa gacgactttt ttattataca acaccgtttt tttcatcgtt gctggccttt
     1441 cataaggggc caagattacc ggatgattaa taatgaatat actatttacg gaatcatcac
     1501 cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg
     1561 ggcattcagt tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat
     1621 tgggaattga tatcacattc gcgttatttc gaaacagtct tcacatccct actgcatgga
     1681 gattactcgg aatagttcat ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg
     1741 atagcaatat tgttggttta gtacggttat tcacttggaa acatccattc agaattatca
     1801 ggcaaaagac atatttgaca caaaaaacaa aagttttttc aataaatcat ttttgcgatg
     1861 aggtgattgt tcccggaaca agtatgaaga cacatctgga gcaggaagga tgtcgaaccc
     1921 gggttactgt tgtgcctcca ggctttgatt tccagaaatt atacgttgat tcccgaaaca
     1981 gtttgcctcc aaatgttctt tcttggctgg cgtcccgaag gggatgcccc gttattgctc
     2041 aggtaggaat gttgcgcccg gaaaaagggc acgaatttat gttgaattta ctgttccatt
     2101 taaaaatgaa tggacgacag ttctgttggt tgattgtggg gtctggttcg cctgaactgc
     2161 gggagcattt acagtatcag attgacagta tgggcatgca tgatgatgtt tttattgctg
     2221 acaatgtttt tcctgccgcc cccgtatatc gggttgccag tctggtggtt ctgccttcag
     2281 aaaacgaatc gtttggtatg gtactggcag aagcatcggc attttctgtg cctgtactgg
     2341 ccagtcagat tggtggaatc cctgatgtta ttcagaacaa ccagaccggg acattgttac
     2401 cagcaggtaa taagcacgca tggatgtgcg ccctgaatga tttttttaat gaccctgggc
     2461 gtttttatca gatggctcgc caggcaaaac aggatataga agagcggttt gatattaata
     2521 aaactgcgtt aaaaatactc acattagcga agcacaagta acaatatgtt ttctgtaagt
     2581 aacttatcat ttatcggttt ccttaaaagg attgtttttt cctcagattc actcccgggg
     2641 aagtgggaac acagaaaatt ccggttcatg tacattttgc gatgtgctat aaatccggtt
     2701 gccagtattc gatattatta cgaactgcgt tccttgccgt gcattgagga tattctggca
     2761 atacacccca cgttgccagc aagaattcat cgcccttatt tgcataaagg ggggcgcgcc
     2821 tggacccgtg gacaatatat tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa
     2881 tattctaaat ttttgtttcc acaaaaatcg gtatcattag tccaattcat cgggaaagat
     2941 ggagagaatt ttgatatcca gtgttcttcc agtggttttg accgggaagg agagttgatg
     3001 ttgtcattgt tttacaataa aacagttatt acccggttaa cattttcagt cattctgact
     3061 caaaatggac acattgcttt tattggcgga ttacagggag cccctaaaaa taccggacct
     3121 gatgttattc ggtgcgcaac ccgggcctgt tatgggattt tcccaaaacg gattattttt
     3181 gaagcatttt gtgcgttgat gaaagcttgc aatatctctg agtgtctggc agtcagtgag
     3241 cacagtcatg ttttccggca attgagatac tggtatcaga aacgcaaaac ctttgttgcg
     3301 gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta
     3361 ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgagtac
     3421 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
     3481 tatcctgtac actcagaaca tcaggattta aattaaggtt tgatgtattc tctgaataaa
     3541 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
     3601 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggtcta catggttcct
     3661 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
     3721 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
     3781 tttatcgctt tgtttcccag aaaagagtga tacagaacgg gaaataattg tcgacaaaat
     3841 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
     3901 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcgatacaa
     3961 taacagaaat gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt
     4021 gctggcagct cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat
     4081 tgattatgtc tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga
     4141 tgggataaaa ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga
     4201 tcaggatcat ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac
     4261 attaccaatt attggacgtc tgatgaacat cagtcaggct atgattatac cgcttttccc
     4321 ggtttatgat gaaaaaaaac atttcctgac tattgaagtt cggccaccaa tggatgcatg
     4381 cattgccagc gcggacaata aaatgattgc ccgacaaatg aacaaaacag tggaaatttt
     4441 ggtggggtca catccggaac agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc
     4501 aaacgaagcg gacccgtacc cttgaaatta taaagtacag gtatttccac tagttgtttc
     4561 ttacaggtta ccaatcgaaa cacatccccc ttccgtgtgt tgcccgtttg gcacggagga
     4621 ctgtgtagat gcagttcgtt aaatttaatt tatgcctaaa tagctgcgct taataccgct
     4681 acacttttgc cagtccatgt ttgcctccgg ggaatgggct gacggtttcc ataaaatggc
     4741 gaactttttt caacagttgc cacattgagc ggcactgatg attacgcgtt attgtgtcgt
     4801 gaagtgcctg ccatagccgt tcagcatgat tcacccatgg cgagtaaacc ggctgataaa
     4861 tgaccctgaa cttcgggttc tccttcagcc agctctgtgt ttcccggctt ttgtggataa
     4921 tgtagttgtc cacgatcagc gtgatggttt tcgcccgacg gtatgtcgct ttaagccgct
     4981 tcagcaggct gatgaacagc gccgaacttt tgctgt
//
LOCUS       DAJVOY010000227         4941 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVOY010000227 DAJVOY010000000
VERSION     DAJVOY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.229"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..237)
                     /locus_tag="OQL90_003808"
     CDS             complement(<1..237)
                     /locus_tag="OQL90_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HCS1370417.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARF"
     gene            495..1274
                     /gene="deoC"
                     /locus_tag="OQL90_003809"
     CDS             495..1274
                     /gene="deoC"
                     /locus_tag="OQL90_003809"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1370418.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1401..2723
                     /gene="deoA"
                     /locus_tag="OQL90_003810"
     CDS             1401..2723
                     /gene="deoA"
                     /locus_tag="OQL90_003810"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1370419.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2775..3998
                     /gene="deoB"
                     /locus_tag="OQL90_003811"
     CDS             2775..3998
                     /gene="deoB"
                     /locus_tag="OQL90_003811"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1370420.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            4078..4797
                     /gene="deoD"
                     /locus_tag="OQL90_003812"
     CDS             4078..4797
                     /gene="deoD"
                     /locus_tag="OQL90_003812"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1370421.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1190 a         1258 c         1359 g         1134 t
ORIGIN      
        1 aaaacgggcg taatcgggta agacatagcg cggtttgtac ggcgcatgac cttcaaacat
       61 atcgcagatt acaccctcat ccagcgcgcg gcgggcttcg gcaggaagct gcggataagg
      121 cagattgttt tctgcttcca gagccagaaa atggcgcttc tgctccgggc taagcactgg
      181 gctggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag tgggcatctt
      241 cttttccttt tatgccgaag gtgatgcgcc attgtaagaa gtttcgtgat gttcactttg
      301 atcctgatgc gtttgccacc actgacgcat tcatttgaaa gtgaattatt tgaaccagat
      361 cgcattacag tgatgcaaac ttgtaagtag atttccttaa ttgtgatgta tatcgaagtg
      421 tgttgcggag tagatgttag aatactaaca aactcgcaag gtgaatttta ttggcgacaa
      481 gccaggagaa tgacatgact gatctgaaag caagcagcct gcgtgcactg aaattgatgg
      541 acctgaccac cctgaatgac gacgacaccg acgagaaagt aattgctctg tgtcatcaga
      601 ccaaaactcc ggtcggcaat accgccgcta tctgtatcta tcctcgcttt atcccgattg
      661 ctcgcaaaac gctgaaagag cagggcaccc cggaaatccg aatcgctacg gtaaccaact
      721 tcccacacgg taacgatgac atcgaaatcg cgctggcaga aacccgtgcg gcaatcgcct
      781 acggtgctga tgaagttgac gttgtgttcc cgtaccgcgc gctgatggcg ggtaacgagc
      841 aggttggttt tgacctggtg aaagcctgta aagaggcttg cgcggcagcg aatgtactgc
      901 tgaaagtgat catcgaaacc ggcgaactga aagacgaagc gctgatccgt aaagcgtctg
      961 aaatctccat caaagcgggt gctgacttca tcaaaacctc taccggtaaa gtggcagtga
     1021 acgcgacgcc ggaaagcgcg cgcatcatga tggaagtgat ccgtgatatg ggcgtagaaa
     1081 aaaccgttgg tttcaaaccg gcgggcggcg tgcgtactgc ggaagatgcg cagaaatatc
     1141 tcgccatcgc agatgaactg ttcggtgctg actgggcaga tgcgcgtcac taccgctttg
     1201 gtgcttccag cctgctggca agcctgttga aagcgctggg ccacggtgac ggtaagagcg
     1261 ccagcagcta ctaagcaaga tgctttacgc ctgatgcgct gcgcttatca ggcctacgag
     1321 acgtatctac ccgtaggccg gataaggcgt agacgcatcc ggcaaaagcc gcctcatact
     1381 cttttcctcg ggaggttacc ttgtttctcg cacaagaaat tattcgtaaa aaacgtgatg
     1441 gtcatgcgct gagcgatgaa gaaattcgtt tctttatcaa cggcattcgc gacaacacta
     1501 tctccgaagg gcagattgcc gccctcgcga tgaccatttt cttccacgat atgacaatgc
     1561 ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc gactggaaaa
     1621 gcctgcatct gaatggcccg attgttgata aacattccac cggcggcgtc ggcgatgtca
     1681 cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg atgatctccg
     1741 gtcgcggcct cggtcatact ggcggtacgc tcgacaaact ggaatccatc cctggcttcg
     1801 acattttccc ggatgacaac cgtttccgcg aaattattaa agacgtcggc gtggcgatta
     1861 tcggtcagac cagctcactg gctccggcgg ataaacgttt ctacgcgacc cgtgatatta
     1921 ccgcaaccgt agactccatc ccgctgatca ctgcctcgat cctggcgaag aaactggcgg
     1981 aaggtctgga tgcgctggtg atggacgtga aagtgggtag cggcgcgttt atgccgacct
     2041 acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc gctggcgtgc
     2101 gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca ggtaacgcgg
     2161 ttgaagttcg tgaagcggtg cagttcctga cgggtgaata tcgtaacccg cgtctgtttg
     2221 atgtcacgat ggcgctgtgc gtggagatgc tgatctccgg caaactggcg aaagatgacg
     2281 ccgaagcgcg cgcgaaattg caggcggtgc tggacaacgg taaagcggca gaagtctttg
     2341 gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt tgagaactac gcgaagtatc
     2401 tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt gtcagtgaaa
     2461 tggatgcccg cgcgctgggg atggcagtgg ttgcaatggg cggcggtcgt cgtcaggcat
     2521 ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc gaccaagtag
     2581 acggtcagcg tccgctggcg gttatccacg cgaaagacga aaacagctgg caggaagcgg
     2641 cgaaagcggt gaaagcggca attaaacttg ccgataaagc accggaaagc acaccaactg
     2701 tctatcgccg tatcagcgaa taacggtata ctgatctgat catttaaatt tgaagcactg
     2761 agtacggaga acatatgaaa cgtgcattta ttatggtgct ggactcattc ggcatcggcg
     2821 ctacagaaga tgcagaacgc tttggtgacg tcggggctga caccctgggt catatcgcag
     2881 aagcttgtgc caaaggcgaa gctgataacg gtcgtaaagg cccgctcaat ctgccaaatc
     2941 tgacccgtct ggggctggcg aaagcacacg aaggttctac cggtttcatt ccggcgggaa
     3001 tggacggcaa cgctgaagtt atcggcgcgt acgcatgggc gcacgaaatg tcatccggta
     3061 aagatacccc gtctggtcac tgggaaatcg ccggtgtccc ggttctgttt gagtggggat
     3121 acttctccga tcacgaaaac agcttcccgc aagagctgct ggataaactg gtcgaacgcg
     3181 ctaatctgcc gggttacctc ggtaactgcc actcttccgg tacggtcatt ctggatcagc
     3241 tgggcgaaga gcacatgaaa accggcaagc cgattttcta tacctccgct gactccgtgt
     3301 tccagattgc ctgccacgaa gaaacctttg gcctggataa actctacgaa ctgtgcgaaa
     3361 tcgcccgtga agagctgacc aacggcggct acaacatcgg tcgtgttatc gctcgtccgt
     3421 ttatcggtga caaagccggt aacttccagc gtaccggtaa ccgtcacgac ctggctgttg
     3481 agccgccagc accgaccgtg ctgcagaaac tggttgatga aaaacacggt caggtggttt
     3541 ctgtcggtaa aattgcagac atctacgcca actgcggtat caccaagaaa gtgaaagcaa
     3601 ctggcctgga cgcgctgttt gacgccacca tcaaagagat gaaagaagcg ggtgataaca
     3661 ccatcgtctt caccaacttc gttgacttcg actcttcctg gggccaccgt cgcgacgtcg
     3721 ccggttatgc tgcgggtctg gagctgttcg accgccgtct gccggagctg atgtctttgc
     3781 tgcgcgatga cgacatcctg atcctcactg ctgaccacgg ttgtgatccg acctggaccg
     3841 gtactgacca cacgcgtgaa cacattccgg tactggttta cggcccgaaa gtaaaaccgg
     3901 gttcactggg tcaccgtgaa accttcgcgg atatcggcca gactctggca aaatattttg
     3961 gtacttctga tatggaatat ggcaaagcca tgttctgata atgggtgcgg cttgatgccc
     4021 tcaccccggc cctctcccac agggagaggg tgaaaacatg ttgataagga taaaacaatg
     4081 gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt gatgccaggc
     4141 gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg tgaagtgaac
     4201 aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat ttccgtaatg
     4261 ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat caccgatttc
     4321 ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca cgtaaaactg
     4381 cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg catccgtttt
     4441 aaagatcatg actttgccgc tatcgctgac tttgacatgg tgcgtaacgc ggtagacgcg
     4501 gctaaagcac tgggcattga tgctcgcgtt ggtaacctgt tctccgctga cctgttctac
     4561 tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg gcatcctcgg cgtggaaatg
     4621 gaagcggctg gtatctacgg cgtcgctgca gaatttggcg cgaaagccct gaccatctgt
     4681 accgtgtctg accacatccg cactcacgag cagaccactg ccgctgagcg ccagaccacc
     4741 ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc tgggcgataa agagtaattg
     4801 tgtttcgctg aaaggcgatt gtcgtgtgaa gccggagtgg gagactgctc cggcttttta
     4861 gtatctattc atcaatagca actgatttat agatttacta ttatttttca tatggataga
     4921 aaataaaatc tattcaagta t
//
LOCUS       DAJVOY010000228         4941 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVOY010000228 DAJVOY010000000
VERSION     DAJVOY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.230"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..902
                     /locus_tag="OQL90_003813"
     CDS             <1..902
                     /locus_tag="OQL90_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCS1370422.1"
                     /translation="FGPRQFTVSFDETLKPFVRDASGSRLKDLPKPNKSDDESRSNDA
                     VNRYKLLKKDARIVAAQQVARQESAMCLRRRWSPENFKLFLVEHPLVRHLTRRLIWGV
                     YSAENQLQACFRVAEDNSYSTADDDLFTLPEGGISIGIPHVLEISPTDAAAFGQLFAD
                     YELLPPFRQLDRNSYALTGAERNASELTRWAGRKCPSGRVMGLANKGWIKGTPQDGGW
                     IGWMIKPLGRWSLVMEIDEGFAVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQE
                     AQFSVIDAITASELINDIEALFE"
     gene            912..1154
                     /locus_tag="OQL90_003814"
     CDS             912..1154
                     /locus_tag="OQL90_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCS1370423.1"
                     /translation="MDKELPWLADNAQLELKYKKSKTPLSHRNWPGEPVSVITESIIQ
                     TLGDELLQKAEKKKRLSGVMKIFHWSGSPPSRRPST"
     gene            2678..4543
                     /locus_tag="OQL90_003815"
                     /pseudo
     CDS             2678..4543
                     /locus_tag="OQL90_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            4605..>4941
                     /locus_tag="OQL90_003816"
     CDS             4605..>4941
                     /locus_tag="OQL90_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370424.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDERKLRGESPEPVPVIFNCELQNIFNQ
                     LIEYYFIKVG"
BASE COUNT         1285 a         1247 c         1255 g         1154 t
ORIGIN      
        1 atttcggccc acgccagttc accgtcagct ttgatgaaac cttaaaaccg tttgtgcgtg
       61 acgcttccgg cagccgcctg aaagacctgc ccaaaccaaa caaaagcgat gatgaatcgc
      121 ggtcgaacga tgcggttaac cgctacaaat tgctgaaaaa agatgcgcgt atcgtcgccg
      181 cccagcaggt agcaaggcag gaatccgcca tgtgcctgcg tcgccgctgg tcgccagaaa
      241 actttaagct cttcctggtt gagcatccgt tggttcgcca cttaacccgc cgtctgattt
      301 ggggcgttta tagcgccgaa aaccagctac aggcgtgctt tagggtggcg gaagataaca
      361 gctacagcac cgctgacgat gatcttttca ccctgccgga aggcggtatc tctatcggca
      421 ttcctcacgt tctggaaata tcaccgacgg atgctgccgc ctttggtcag ctttttgccg
      481 actacgaact gctaccacca ttccgtcagc ttgaccgtaa cagttacgcc ctgacaggag
      541 ccgagcgcaa cgccagcgaa ctgacccgct gggcaggcag aaaatgcccg agcggtcggg
      601 tcatggggct ggcgaataaa ggctggataa agggcacccc gcaggatgga ggctggatcg
      661 gctggatgat caaacccctg ggccgctggt cgttagtcat ggaaatcgat gaaggttttg
      721 cggtaggcat gtcgccagcc gaactcagcg ctgagcagct cttaagcaag ctgtggctat
      781 gggaaggcaa agcagaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct
      841 ccgtaatcga tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat
      901 aaggaaagag catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga
      961 aatataaaaa aagcaaaacg ccgctcagtc atcgcaattg gccgggcgaa ccggtgtctg
     1021 ttatcactga aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga
     1081 aaaaaagatt gtctggcgtt atgaaaattt ttcactggag tggcagtccg ccatcacgca
     1141 ggccatcaac ttgatcggcg aacacaaacc ctcagtcccg gcccggacaa tggcggcgct
     1201 agcctgtatc gcgcaaaatg acagccaaca gttgctcgac gaaatcgtcc aacaagaggg
     1261 gctggaatat gcgactgagg tggtaattgc acgcctgttt attgcgcggt gttatgagag
     1321 tgatcctctg gtagtgacat tgcagtatca ggacgaggat tatggctatg gttatcgctc
     1381 agaaacctat aacgaattcg atctccgact gcgtaagcat ctctctctgg cagaggaaag
     1441 ctgctggcag cgttgcgccg acaaactcat tgccgcacta ccaggaataa ccaaagttcg
     1501 ccgccctttt attgcgctga tcctcccgga aaaaccagaa atagccaatg agttggtagg
     1561 ccttgaatgc ccgcgaactc attttcattc taagaagtgg ttaaaagttg ttgctaatga
     1621 ccccacagcg gtgagaaaac tcgaacacta ctggagccag gatatattta gcgatcgaga
     1681 agccagctac atgtcgcatg aaaaccactt cggctacgcg gcctgcgccg cccttttgcg
     1741 cgaacaagga ctggcagcca ttccgcgcct cgcgatgtat gcccataaag aagattgcgg
     1801 cagtctgctg gtacaaatta accatccgca agtcatccgc accttgctac tggtggctga
     1861 taaaaacaaa cccagcctgc aacgtgtagc taaataccat aaaaacttcc cccatgcgac
     1921 gctcgccgca ctggcagaac tgctggcgtt aacagaacca ccagcccgcc ctggttatcc
     1981 aatcatcgaa gacaaaaagc tgcctgcaca gcaaaaagca cgcgatgaat actggcgtac
     2041 gctgttacaa acgctgatgg catcgcagcc acaactggca gaagaggtga tgcagtggtt
     2101 aagtactcaa gccagggcag tgctgaatag ttatttatcg gcaccgccca aaccggttat
     2161 tgatagtacc gataacagca atctgcctga aatcctcgtt tcgctaccgt ggcgtagtaa
     2221 gaaaaaaatg acagctccac gtcttgattt ggcaccgctc gaattaactc cgcaagttta
     2281 ctggcaacca ggcgaacaag agaggcttgc cgccactgag tctgcccgtt atttcagcac
     2341 ggaatctctt gcgcaacgca tggaacaaaa aagtggacga gttgtattac aggaactggg
     2401 ttttggggat gatgtatggc tgtttctgaa ttatatactc cccggaaaac tggatgctgc
     2461 acgcaattca ctcattgttc agtggcatta ctaccagggg cgggttgaag agatcctgaa
     2521 tggctggaac tccccggaag cacaattagc agaacaggcg ctccgcagcg gtcacataga
     2581 agcgttaatt aacatatggg aaaatgacaa ctactcacgt tatcgtccgg aaaagagtgt
     2641 ctggaacctg tatttattgg cacagttgcc gcgtgagatg gctttgacct tctggctgcg
     2701 tatcaatgag aaaaagcatc tgttcgcggg tgaggactat tttctcagta tcctcggatt
     2761 ggatgcgcta ccaggtctgc tgttggcttt ttcacatcgt ccaaaagaaa catttccgtt
     2821 aattttaaat tttggcgcaa cagaactggc gctgcctgtt gcccgcgtct ggcgacgttt
     2881 cgcggcgcag cgtgatctgg ctcgccagtg gattttacaa tggccggaac atacggctag
     2941 tgcacttatc cctcttgtct ttaccaaacc cagcgataat agcgaagccg cattacttgc
     3001 cctgcgttta ctgtacgaac agggacatgg cgaattgcta caaaccgtgg caaaccgctg
     3061 gcagcgtaca gatgtatggt ctgccctgga gcagttgctt aaacagggtc caatggacat
     3121 ttacccggca cgcattccaa aagcccctga tttctggcat ccggcaatgt ggtccaggcc
     3181 tcgacttatc actaataatc agcctgttac cggtgacgct ctggaaatta tcggcgaaat
     3241 gctgcgcttt acctaggggg gacgttttta tagcgggctg gaacaactga aaacgttctg
     3301 ccagccacaa acgctggccg cttttgcctg ggatctcttc actgcctggc aacaagcggg
     3361 ggcccccgca aaagacaact gggcatttct ggcattacgt ctctttggtg acgaaagcac
     3421 ggcacgggat ctgacgacac agatcctcgc ctggccacaa gaaggcaaat ctgcccgtgc
     3481 tgtcagtggt ctgaatatac ttaccctgat gaataatgat atggcgctga tgcagctgca
     3541 tcatgtatca caacgggcga aatcacgccc cttacgtgaa aacgcagcgg aatttcttca
     3601 ggtggtcgca gaaaatcgcg ggctaagcca ggaagagtta gcggacagat tagtcccaac
     3661 cctgggcctt gatgatccgc aggcgttgag ttttgatttt ggtccccggc agtttaccgt
     3721 tcgcttcgat gaaaacctca acccggttat ctttgatcag caaaacgttc gccagaaaag
     3781 cgttccccgg ttgcgcgccg atgacgatca actgaaagcg cccgaggcac tggcccgact
     3841 aaaagggcta aaaaaagatg ctactcaggt gagcaaaaac ctgctcccgc gtcttgaagc
     3901 tgccctacgt accacccgac gctggtcgct ggcagatttt cattctctgt ttgttaatca
     3961 tccctttacc cgtctggtta cccagcgatt aatatggggg gtttatccgg caaatgaacc
     4021 gcgttgttta ctcaaagcct ttcgtgtggc cgcagagggg gagttctgca atgcgcaaga
     4081 tgagccaatt gacctgcctg cggacgctct gattggcatt gcccacccgt tagaaatgac
     4141 agtagaaatg cgcagtgaat ttgcacagct ttttgccgat tacgaaatta tgccgccttt
     4201 tcgccaattg tcgcgccgca cggtgctgct cacacctgac gagtcaacca gtaacagcct
     4261 gactcgctgg gaaggtaaat ccgctaccgt tgggcagctt atgggaatgc gatacaaagg
     4321 ctgggagtca ggttatgagg acgcatttgt ctatgacctg ggtgagtacc ggctggtcct
     4381 taagttttca cccggtttta accactacaa tgttgatagc aaagcgctaa tgagcttccg
     4441 ttctcttcga gtgtaccgtg acaataaatc cgtcactttt gccgaacttg atgtgtttga
     4501 tttgagtgag gcgttaagcg cacctgacgt cattttccat taaaaacaca gcgggcagtg
     4561 atgcaactgc ccgttatcaa cacgacttac cagcggggaa agcgatgatc gtgcaaaaag
     4621 agctggttgc tatttacgat tatgaggtcc ctgtacctga aaatccgttt tccttcagac
     4681 ttgagatcca taaatgctct gaattattta caggttccgt ctatcgactg gaacggttcc
     4741 ggctacgtcc aacatttcat caacgtgatc gagaggatgt tgacccgcta ataaatgatg
     4801 cgttgattta tataagagat gagtttattg atgagcggaa attacgaggt gaatcacctg
     4861 aacctgtacc agtaattttt aattgtgaac tacagaatat attcaaccaa ctaatagaat
     4921 attactttat taaagtggga a
//
LOCUS       DAJVOY010000229         4914 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVOY010000229 DAJVOY010000000
VERSION     DAJVOY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.231"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            718..2571
                     /locus_tag="OQL90_003817"
     CDS             718..2571
                     /locus_tag="OQL90_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1370425.1"
                     /translation="MAFKHYDVVRAASPSDLAKQLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTVGLNALTLTDITKNATYGVEIESLVLEINAPASS"
     gene            2721..2936
                     /locus_tag="OQL90_003818"
     CDS             2721..2936
                     /locus_tag="OQL90_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1370426.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2941..3291
                     /locus_tag="OQL90_003819"
     CDS             2941..3291
                     /locus_tag="OQL90_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCS1370427.1"
                     /translation="MTQNYKLIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            3355..3888
                     /locus_tag="OQL90_003820"
     CDS             3355..3888
                     /locus_tag="OQL90_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1370428.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLSEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            4187..4624
                     /locus_tag="OQL90_003821"
     CDS             4187..4624
                     /locus_tag="OQL90_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1370429.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GNGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT         1267 a         1161 c         1350 g         1136 t
ORIGIN      
        1 ctgtgccgga tacatcacag tttctggaac cttaggtact acagtatcag tcagggtgct
       61 atattttcag atgtgatgaa agcctgtcag caggcagggc gtatcggaaa tgacccagta
      121 gagaaaacgt tgactcagat accggtgctg agttaccggg aaaccggcat cacatgaccg
      181 ctatccttcc aggcccatcc gctccggtgg gcctttttac tgcagaaaac aggttccccg
      241 ttaaatgcta tgttgctcac aattcagtaa gttgacagtt gcctgtcaga ctgggcattt
      301 gttaaaaaaa tttcgcatgg tgaatccccc tgagcggagg ggcgactggt gacggtataa
      361 tctctgatta tcaaaacgag aatgacgcgg gtttagtggc accgggctga actcaccggg
      421 aggcacccgg caccatgtgc atgatgatac agatacgcgg ctttagcccc tctccggagg
      481 ggctttctta tggacaaaaa aagcccgcgc tgggagacgc gggcggcaag gaataaacaa
      541 taaaacgtga agtaatattt cagctggcga ataatacccc atagtaatca ctctgcgcaa
      601 ctgcgcggtc tttttcgaat tgcgggctgt agtctccctt ctgccattgt cctgtaactt
      661 ccggacttca gcctgctcct tatctgactc acaacattat cccgcccggg aggattcatg
      721 gcatttaaac actatgacgt ggtcagggcg gcatcgccgt cagaccttgc gaaacaactg
      781 acacaaaaac tgaaggaggg ctggcagccg tttggtagtc cggtggccat aaccccttat
      841 accctgatgc aggcgattgc agcagaaggt gatgtggtcg tcagtggtgc aactgagccg
      901 gagtggtact acgtcatcgt actggccggg caatccaatg ccatggctta cggtgaaggg
      961 cttccgcttc cggattcata cgatgcgccc catccgcgca ttaagcaact ggcccgtcgt
     1021 aacacagtga ctcccggtgg tgaagtatgc gtatttaacg acatcattcc tgctgaccat
     1081 tgtctgcatg atgttcagga tatgagtacg attaaccatc cccgggctga cctgagcaaa
     1141 gggcagtacg gctgtgtcgg acagggctta catattgcca aaaaactgct tccgtatatc
     1201 cctaataatg cggggatcct gctggtacca tgctgtcgtg gtggttcggc attcacccag
     1261 ggcacggagg ggacattcag cgagtccacg ggagccagtc aggattcggc tcgctgggga
     1321 gtgggtaagc cgttatatca ggatctgctt ttccgcacga aggcagcatt gcagaaaaac
     1381 ccgaaaaacg ttttgctggc gatatgctgg atgcaggggg aattcgatat gacgaatgcc
     1441 agttacgccc agcagccagc agcatttctt gcaatggtac agcagttccg tgctgacctt
     1501 gccgggctgg cagcgcagtg tcacggtgga agtccggcat cagtcccctg gatttgtggc
     1561 gacacgacat acgcgtggaa acaagaacac ggtacgcaat atgaagtggt atatggtgca
     1621 tataaaggta aagaatccca gcagatttat tttgttccct ttatgaccga tggtagcgga
     1681 gttaatacac cgacaaacaa cccgtcagaa gatcctgata ttgccgggtc tggttattac
     1741 ggttcggcat cccgaacgaa caaaaactgg gtatcatcaa atcgcccgac gcatttcagc
     1801 tcatgggcgc gtcgtggcat tattcccgat cgtatggcaa ctgctattct gaacgtagcc
     1861 ggtcgcacct tagccttcat tagtggtaag gcaccggaaa tcaaaccctc gcccggcggc
     1921 gacacgccat cggggccgtc tgaagatgca tccgtacgca caatctccct gttgccgaca
     1981 gccggagatg ctgctgcgca gggctggagc attaagaatg gcggaattca gttgtcagat
     2041 ggtgtattta agatcaccaa gcagagcaat aaagcctggt ccctgacgcg cccggtggat
     2101 gacgcagtct ccctgctgac acggggtggc agactgagct gtaagtttcg actgtcaggc
     2161 gcactgacca acaaccagtt cggtctggga atttatctgt ataccgatgt agcgttacct
     2221 gacgtcgtgg cgatgaccgg gactggtaac ccgttcctga tgtcgttctt cacccagacc
     2281 acagacggca aactgaatct gatgcatcac aagaaagccg gaaacacaaa gttgggcgag
     2341 ttcgggaatt acagtaacga ctggcagacg ctggagctgg tgttcaccgc cggcagtgcc
     2401 acggttactc cgaaactgaa tggagtggct ggcccggcat tccaggtcat aaaagacagt
     2461 ctgacagtgg gactaaatgc actgacgctg acggatatta ccaaaaatgc aacgtatggc
     2521 gttgagatag aaagtctggt gctggagata aatgcaccgg catcatcata aaaagtgagc
     2581 cagtcaaatg gaaggtatcg ttaaactcac cggtagtgtc agtgggtcgt ctgagacgcc
     2641 tgcatgagtt atcagagcca tcagtactta actggtggct tttttattgt tgtcagcttc
     2701 cggataacgg gagacggggt atgtaccaga tggaaaaaat cacaacaggt gtgtcataca
     2761 ccacgtcagc ggtgggaacg ggctactggt tcctgcagtt gctggacagg gtttccccgt
     2821 ctcagtgggc ggcaataggc gtgctgggga gtctgctgtt tgggctgctg acatatctga
     2881 ctaacctgta tttcaaaatc agagaggacc gtcgtaaggc tgcacgggga gagtaattca
     2941 atgactcaaa actataaact gattgtgaaa gggatccgca attttgagaa taaagttacg
     3001 gtaactttag cgttacggga caaaaaacgc tttgacggtg aaatttttga cctggacatc
     3061 tcgctggacc gtgttgaagg tgccgcgctg gagttttatg aggcagcagc cagaaggagc
     3121 atcagacagg tcttcctgga tgttgctgcc gggttatgtg aaggggatga gcagtcgccg
     3181 gaaaagcgcc ccgtaatttt agaggcgcag aatgtatgga taacctacaa aggaaagcta
     3241 ccgggaagaa ttactggttc tctgaagact cctccggaat cacaacctta agtcactgac
     3301 cggaacagat aaacctgtcc gtgggcagaa accgataaat cctgataaat atccatgaac
     3361 gcaaaaatca gatacggcct gtcggctgcc gttctggcgc tgattgccgc tggtgcgcct
     3421 gcgcctgaca ttctcgacca gtttctggat gaaaaggaag gtaaccacac cacggcatac
     3481 cgtgatggcg cgggtatctg gaccatttgc cgcggtgcca tcctggtgga tggcaaacct
     3541 gtcgttccgg gcatgaagtt gtcgaaggaa aaatgcgacc gggttaacgc cattgagcgt
     3601 gataaggcgc tggcatgggt ggagaaaaac atcagagtgc cattgagtga accccagaaa
     3661 gcggggatcg cgtcattctg tccgtacaac attggccccg gtaagtgttt tccgtcgacg
     3721 ttttataaac gaattaatgc aggtgatcgc aggggagcgt gtgaggcgat tcgctggtgg
     3781 attaaggacg gtggcagaga ctgccgtatt cgctcaaaca actgttacgg tcaggtatcc
     3841 cgtcgcgacc aggagagtgc gctggcgtgc tggggaattg acagataagc agaatatttt
     3901 gctgaaaaat gcggtttgct cacacggacg gataacacga aatcctgcga actgacaaaa
     3961 actaagagaa taaaagtaaa aaccccgttt gttggctgca agcggggttt tgtgtttcct
     4021 gactccggaa aagtcaaagg agaaagtgtg tttgatttta gcaaactgat tcgggagatt
     4081 cgagtgatgg ctgaaaaatt atccacctgg aagttcattc ttatctggct ggtgtttgtg
     4141 attatggcct ccggttattt catcggtcag atacgctggt ggtgaaatga accgcgtact
     4201 gtgcgtggtc atcattgccc tgctggtggc ctgtggtgcg cttagtctgg ggctgaatca
     4261 ttaccgtgat aacgccataa cctacaaaga gcagcgcgat aaagccacat ccacaatcgc
     4321 tgatatgcag aagcgtcaac gtgacgtagc agaactcgat gccagatata caaaggagct
     4381 tgctgatgct aacgcgacta tcgaaagcct ccgtgctgat gtttctgctg ggcgtaagcg
     4441 cctgcaagtc gccgctacct gtgcaaagtc aacgaccgga gccagcggca tgggcaatgg
     4501 agaaagccca agacttacag cagatgctga actcaattat taccgtctcc gaagtggaat
     4561 cgacaagata accgcgcagg tcaactacct gcaggagtac atcaggacgc agtgcttaaa
     4621 ataattttaa tttcactgaa atttaatacg tgactttcag gaaaatgcct cgcagatgcg
     4681 gggcattttt gtacaggtat ttcaccgcgc accgcagtgc actcaaccac gtcgaaccaa
     4741 accctttgga atgagccttt gagtagtcag ttagtgctgg tgagccttga cgggctgatc
     4801 tcctatgcag caaaggttca tctcaaagta aggcgaacgc tatgacaaac caagattcta
     4861 tagacctatc tgatcttcgt ggaatggtca gttttccaga caaaaggtaa tcac
//
LOCUS       DAJVOY010000230         4852 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVOY010000230 DAJVOY010000000
VERSION     DAJVOY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.233"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            298..1272
                     /gene="hemB"
                     /locus_tag="OQL90_003822"
     CDS             298..1272
                     /gene="hemB"
                     /locus_tag="OQL90_003822"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1370430.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1378..2229)
                     /gene="tauD"
                     /locus_tag="OQL90_003823"
     CDS             complement(1378..2229)
                     /gene="tauD"
                     /locus_tag="OQL90_003823"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1370431.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(2244..3053)
                     /gene="tauC"
                     /locus_tag="OQL90_003824"
     CDS             complement(2244..3053)
                     /gene="tauC"
                     /locus_tag="OQL90_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1370432.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            complement(3050..3817)
                     /gene="tauB"
                     /locus_tag="OQL90_003825"
     CDS             complement(3050..3817)
                     /gene="tauB"
                     /locus_tag="OQL90_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1370433.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3830..4792)
                     /gene="tauA"
                     /locus_tag="OQL90_003826"
     CDS             complement(3830..4792)
                     /gene="tauA"
                     /locus_tag="OQL90_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1370434.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQAGNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKLGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1138 a         1404 c         1227 g         1083 t
ORIGIN      
        1 acgcgttata aatatcaaaa acacaaacca gataaatatc ttatcaatat acaacaaagt
       61 ttgaattatt caatttatag cagcacaacc aaacgcaaaa aaaaataata accaagagct
      121 ataaataaag attaaaaata ttttcagtga atatgaactt attgctattt ctattacaaa
      181 gatgtcctgg tctctctttc attctcttct tgtcaacatc acgacaactt tcgtaaaaca
      241 ctcctgcccc gcttcaggta tactgtgcac cttaattcca caaacatcag gcagaccatg
      301 acagacttaa tccaacgccc tcgtcgcctg cgcaaatctc ctgcgctgcg cgctatgttt
      361 gaagagacaa cacttagcct taacgacctg gtgttgccga tctttgttga agaagaaatt
      421 gacgactaca aagccgttga agccatgcca ggcgtgatgc gcattccaga gaaacatctg
      481 gcacgcgaaa ttgaacgcat cgccaacgcc ggtattcgtt ccgtgatgac tttcggcatc
      541 tctcaccata ccgatgaaac cggcagcgac gcctggcggg aagatggact ggtggcgcgt
      601 atgtcgcgca tctgcaagca gactgtgcca gaaatgatcg tcatgtcaga cacttgtttc
      661 tgtgaataca cttctcacgg tcactgcggt gtgctgtgcg agcatggcgt cgacaacgac
      721 gcgactctgg aaaatttagg caagcaagcc gtggttgcag ctgctgcagg cgcagacttc
      781 atcgcccctt ccgccgcgat ggacggccag gtacaggcga ttcgtcaggc gctggacgct
      841 gcaggcttta aagatacggc gattatgtcg tattcgacca agttcgcctc ttccttttat
      901 ggcccgttcc gtgaagctgc cggaagcgca ttaaaaggcg accgcaaaag ctatcagatg
      961 aaccctatga accgtcgcga ggcgattcgt gagtcactgc tggatgaagc ccagggcgca
     1021 gactgcctga tggttaaacc tgctggagcg tacctcgaca tcgtgcgtga gctgcgtgaa
     1081 cgtactgaat tgccgattgg cgcgtatcag gtgagcggtg agtacgcgat gattaagttc
     1141 gccgcgctgg cgggtgctat agatgaagag aaagtcgtgc tcgaaagctt aggttcaatt
     1201 aagcgtgcgg gtgcggatct gattttcagc tactttgcgc tggatttggc tgagaagaag
     1261 attctgcgtt aatttttttc ctctcagaaa gggggagtta acctgttgaa agtacggtga
     1321 acgccccctc accctaaccc tctccccaag ggggcgaggg aaccgtccgc actcgtatca
     1381 ccccgcccga taaaacggtt tatccccaag aatcgtcacc cgatgcataa tccgccgctg
     1441 tggcaggtaa tcggcattgg catagtgctg agtcacgcgg ttatcccaaa tcgcaatatc
     1501 attcggttgc cagcgccaac gcacctgaaa ctccggttta gtgatatggg cgaataaaaa
     1561 gcctaacaag gcttcgctct ctttctcgct gacatcgaca attcgcgtag taaagccttc
     1621 attcacaaac agcgcctgtt taccgctcac cggatgcgtt cgcaccaccg gatgtagcaa
     1681 cggaggattt ttcgcgaccg cctcgcgcca gcgttgatgt tcctcctcgg ttttgcggta
     1741 tttgtattcc gggaacgatt tacggaaatc atgctccgca cgcagcccgc tcagcagctg
     1801 gcggaaggga acagagagcg cctcataggc cgtaataccg ctggtccaga gcgtatcacc
     1861 gccggtcgaa ggtaattctt tagctgccag aatcgcccct gcgggtggcg tttcaataaa
     1921 tgtcacatcg gtatgccagt tgtcgttatc tggcggatta tcgttatggg tatccagcac
     1981 gatgatctcg tcaacccctt cggcatgagg gtaaacaggg tgaatatgca gttcgccaaa
     2041 acgctgggcc agcgcgcgtt gctgctgcgg cgtaatggct tgatcgcgca gaaacaccac
     2101 ctgatggcgc agcaccgcat ggtaaagctg ttcaaactga ttatcgctta acgggcgcgt
     2161 caggtcggca cccgaaattt gtgcgccgat atacggcccc agcggggtaa tgctcagacg
     2221 ttcactcatt gtacttctcc atgctagggc gtcagacggc gctgtaacgc gcgcaaaccc
     2281 agttctaaaa gaaaggcgat aatcgcaatc accgcgattc ccgccagcac cacgtcagtc
     2341 gcgagaaatt cacccgctga ctgaaccata aatcctaaac cgcgcgtcgc ggcaatcagc
     2401 tccgccgcta ccagcgtaga ccagcccaca cccagaccaa tgcgtaatcc ggtgaggatt
     2461 tccggcagcg cgccgggcaa aatgacaaac cacagcacct gcgcacggct ggcacccagc
     2521 gactgggcgg cacgaatgcg aacctgctgc gcgcttttca cccccgccag cgccgacatc
     2581 gccaccggtg caaaaatcgc taaatagatc agtaagatct tcgaggtttc accaatacca
     2641 aaccagatca ccatcagcgg caaataagcc agcggcgaca ccggacgata aagttcgatt
     2701 accggatcca gaatgccgcg caccgtcggg cttagcccca tggcgatccc gaccggaata
     2761 ccgatcacca ccgccgcgag tagcgccagc acaatgcgcg tcaggctggc tgccagatgc
     2821 tgccacagcg tggcgtccat aaatccctgc ggtccggcaa tggtgagcag ttttgccagt
     2881 acttgttgcg gcggcggcaa aaacagcggg ctaatcagtt gcagcgccgc caccgtccac
     2941 catacagtga gtaaaaccgc taacgtgcca atgcttaagg tcacctgacg cgagagcggc
     3001 cagcgccatt tcagccgccg cgaatgcagt ttttcattaa tgagcacact catgagaacg
     3061 cctcccgttg ctcaaatacg cggcttaaaa catattcgcg catggcgatg aattgtggat
     3121 cggacttgat gctgcggctc gactctcccg caacaaagcg gcgagcaaag ttgagcggca
     3181 gccgctccag cacacggcca gggccggatg aaagcagaac cagttcagtc gccataaaca
     3241 ccgcttcttc tatatcgtgg gtaatcaaca gcacctgctt gcccgtctcc tgccagagtt
     3301 tcagcagcag agtttgcatc tggtcgcggg tgaaggcgtc cagcgcacca aacggttcgt
     3361 cgagtaataa cagctgggga ttcgccgcca gcgcgcgggc aatccccacc cgctgacgtt
     3421 gaccaccgga aagctgccag atatagcgtt tttctgcgcc ttccagcccc acttttttca
     3481 gcacctggtg cgcgatttcc agtcgctgca ttttctctat acctgccaat tgcaggccga
     3541 acgccacgtt gtcctgtaca ttgcgccacg gtagcagccc ttcattctga aaaaccaccc
     3601 cgcgctctgc tcccggtccc tcaatacgct tacccgccag ttgaatgctg ccatgctgat
     3661 aaggcacaaa accggcaatc agattcagca acgtggtttt accgcagccg gacggcccca
     3721 gcaccaccag tagctcgccg ctttccagcg tcaggttgat atcttccagt gccggtttgc
     3781 cgccataatc ggcatagaga tgagagattt gcagcatctg cgcctccttt tattgcacga
     3841 agcgcgaggt aacgtactgg ctgtaatcat tcgctacagc cgggaccttg ccctgctctt
     3901 tcaaaaactg cgcggtgtcg atgattactt tgttcaccgg tccggtcagt tctgccgttt
     3961 gttgctgcgg cgtcagatag gtattcccct taaccagccc cggaacgtca ccttccggca
     4021 cgccgcttaa acgcgccagt ttgctgatgt tttccggctg tttcagccac gcgtctgggt
     4081 tagcaatgta cggttgctga gcatcgatgg cgcttttagc gaacgctttc acgacctcag
     4141 gatgtttctc ggcaaaatct ttgcgcacca cccagacgtc cagcgttggc gcaccccact
     4201 gcccgacctg ttcagaatcg gtcaacacct tgccgtcttt ttccagggcg ttaaccgccg
     4261 gtgcccagac ataagcacca tcaatatcac cccgctgcca tgcggcgata atcgcgggcg
     4321 gctgcaggtt cacaatctcc acttgcccta gtttaatccc ccagtgtttc agcgccgcca
     4381 gcaggctgta gtgggtggtg gagataaacg gtacggcaat gcgtttgcca atcaaatctt
     4441 cgggtttgct gatagttttc tttaccacca gcgcttcgga gttacccagt tttgacgcca
     4501 gcaagaagac ttcaatcggc acctgttggc tggctgcaac cgctaatggg ctggaaccga
     4561 ggttgccgat ttgcacgtcg ccagaagcca gcgcccgcac gatgctggct ccgctgtcaa
     4621 acttacgcca gtccacgctc gccccgctct ctttagcaaa ggtgttgccg gcctgagcca
     4681 ctttcgccgg ttcggctgag gtttgatacg ccacggtgac gttcaccgcc tgtgcctgaa
     4741 aagcgatgaa tgccagtgcg gcaagaagtg tgttacgcga tgaaattgcc atgattgtct
     4801 gctccccagt cttgttatgg gagcagtatt caggaataaa aacattcatt aa
//
LOCUS       DAJVOY010000231         4833 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVOY010000231 DAJVOY010000000
VERSION     DAJVOY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.234"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            162..566
                     /locus_tag="OQL90_003827"
     CDS             162..566
                     /locus_tag="OQL90_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCS1370435.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(544..1167)
                     /gene="tetR(B)"
                     /locus_tag="OQL90_003828"
     CDS             complement(544..1167)
                     /gene="tetR(B)"
                     /locus_tag="OQL90_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1370436.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1249..2454
                     /gene="tet(B)"
                     /locus_tag="OQL90_003829"
     CDS             1249..2454
                     /gene="tet(B)"
                     /locus_tag="OQL90_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1370437.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2567..3160)
                     /gene="tetC"
                     /locus_tag="OQL90_003830"
     CDS             complement(2567..3160)
                     /gene="tetC"
                     /locus_tag="OQL90_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1370438.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3293..3664
                     /locus_tag="OQL90_003831"
     CDS             3293..3664
                     /locus_tag="OQL90_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1370439.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3674..>4833)
                     /locus_tag="OQL90_003832"
     CDS             complement(3674..>4833)
                     /locus_tag="OQL90_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCS1370440.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1295 a          914 c         1039 g         1585 t
ORIGIN      
        1 taagttggag tcattaccct gtggcgaata tatcagcttc aacggcgagc agtcatttat
       61 ctaaattatt agattgccag ctaatcacag tagtagctca aggcaagcat cgttattttc
      121 ggctagcagg aaaagatatt gctgaattga tggaaagtat gatggggatc tccttaaacc
      181 atggcgtaca tgccaaagtt tccacgccag tgcatttacg aaaagcacgt acttgctatg
      241 atcatttagc tggcgaagtt gccgttaaga tctatgattc cctttgtcaa cagcaatgga
      301 tcactgaaaa tggttcaatg atcacattaa gtggtattca atattttcat gaaatgggaa
      361 ttgacgttcc ttccaaacat tcacgtaaaa tctgttgtgc gtgtttagat tggagtgaac
      421 gccgtttcca tttaggtggg tacgttggag ccgcattatt ttcgctttat gaatctaaag
      481 ggtggttaac tcgacatctt ggttaccgtg aagttaccat cacggaaaaa ggttatgctg
      541 cttttaagac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      601 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      661 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      721 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      781 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      841 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      901 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
      961 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
     1021 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
     1081 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
     1141 cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga cactctatca
     1201 ttgatagagt tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg
     1261 acaaagatcg cattggtaat tacgttactc gatgccatgg ggattggcct tatcatgcca
     1321 gtcttgccaa cgttattacg tgaatttatt gcttcggaag atatcgctaa ccactttggc
     1381 gtattgcttg cactttatgc gttaatgcag gttatctttg ctccttggct tggaaaaatg
     1441 tctgaccgat ttggtcggcg cccagtgctg ttgttgtcat taataggcgc atcgctggat
     1501 tacttattgc tggctttttc aagtgcgctt tggatgctgt atttaggccg tttgctttca
     1561 gggatcacag gagctactgg ggctgtcgcg gcatcggtca ttgccgatac cacctcagct
     1621 tctcaacgcg tgaagtggtt cggttggtta ggggcaagtt ttgggcttgg tttaatagcg
     1681 gggcctatta ttggtggttt tgcaggagag atttcaccgc atagtccctt ttttatcgct
     1741 gcgttgctaa atattgtcgc tttccttgtg gttatgtttt ggttccgtga aaccaaaaat
     1801 acacgtgata atacagatac cgaagtaggg gttgagacgc aatcgaattc ggtatacatc
     1861 actttattta aaacgatgcc cattttgttg attatttatt tttcagcgca attgataggc
     1921 caaattcccg caacggtgtg ggtgctattt accgaaaatc gttttggatg gaatagcatg
     1981 atggttggct tttcattagc gggtcttggt cttttacact cagtattcca agcctttgtg
     2041 gcaggaagaa tagccactaa atggggcgaa aaaacggcag tactgctcgg atttattgca
     2101 gatagtagtg catttgcctt tttagcgttt atatctgaag gttggttagt tttccctgtt
     2161 ttaattttat tggctggtgg tgggatcgct ttacctgcat tacagggagt gatgtctatc
     2221 caaacaaaga gtcatcagca aggtgcttta cagggattat tggtgagcct taccaatgca
     2281 accggtgtta ttggcccatt actgtttgct gttatttata atcattcact accaatttgg
     2341 gatggctgga tttggattat tggtttagcg ttttactgta ttattatcct gctatcgatg
     2401 accttcatgt taacccctca agctcagggg agtaaacagg agacaagtgc ttagttattt
     2461 cgtcaccaaa tgatgttatt ccgcgaaata taatgaccct cttgataacc caagagggca
     2521 ttttttacga taaagaagat ttagcttcaa ataaaaccta tctattttat ttatctttca
     2581 agctcaataa aaagccgcgg taaatagcaa taaattggcc ttttttatcg gcaagctctt
     2641 ttaggttttt cgcatgtatt gcgatatgca taaaccagcc attgagtaag tttttaagca
     2701 catcatcatc ataagcttta agttggttct cttggatcaa tttgctgaca atggcgttta
     2761 ccttaccagt aatgtattca aggctaattt tttcaagttc attccaacca atgataggca
     2821 tcacttcttg gatagggata aggtttttat tattatcaat aatataatca agataatgtt
     2881 caaatatact ttctaaggca gaccaaccat ttgttaaatc agtttttgtt gtgatgtagg
     2941 catcaatcat aattaattgc tgcttataac aggcactgag taattgtttt ttatttttaa
     3001 agtgatgata aaaggcacct ttggtcacca acgcttttcc cgagatctca tctattgaaa
     3061 cagcttgata gcctttttca acaaacaata ttcgtgctga gttaaccagt gattgatagg
     3121 tactcttaaa attttcttgt tgatgatttt tattttccat gatagattta aaataacata
     3181 ccgtcagtat gtttatggta tcatgatgat gtggtcgtga caatcttaag aacatttagg
     3241 ttattttatg tatattgaac agcattctcg ctatcaaaat aaagctaata acatccaatt
     3301 aagatatgat gataagcagt ttcatacaac ggttatcaaa gatgttctat tatggattga
     3361 acataattta gatcagtctt tactgcttga tgatgtggcg aataaagcgg gttataccaa
     3421 gtggtatttt cagcggctgt tcaaaaaagt aacaggggtc acactggcta gctatattcg
     3481 tgctcgtcgt ttgacgaaag cggctgttga gttgaggttg acgaaaaaaa ctatccttga
     3541 gatcgcatta aaatatcaat ttgattccca acaatctttt acacgtcgat ttaagtacat
     3601 ttttaaggtt acaccaagtt attatcggcg taataaatta tgggaattgg aggcaatgca
     3661 ctgagagatc ccctcataat ttccccaaag cgtaaccatg tgtgaataaa ttttgagcta
     3721 gtagggttgc agccacgagt aagtcttccc ttgttattgt gtagccagaa tgccgcaaaa
     3781 cttccatgcc taagcgaact gttgagagta cgtttcgatt tctgactgtg ttagcctgga
     3841 agtgcttgtc ccaaccttgt ttctgagcat gaacgcccgc aagccaacat gttagttgaa
     3901 gcatcagggc gattagcagc atgatatcaa aacgctctga gctgctcgtt cggctatggc
     3961 gtaggcctag tccgtaggca ggacttttca agtctcggaa ggtttcttca atctgcattc
     4021 gcttcgaata gatattaaca agttgtttgg gtgttcgaat ttcaacaggt aagttagttg
     4081 ctagaaccca tggctccttt gccgacgctg agtagatttt aggtgacggg tggtgacaat
     4141 gagtccgtgt cgagcgctga ttttttcggc ctttagagcg agatttatac aatagaattt
     4201 ggcatgagat tggattgctt ttagtcagcc tcttatagcc taaagtcttt gagtgactag
     4261 atgacatatc atgtaagttg ctgataggtt tccagttttc cgctcctagg tctgcatatt
     4321 gtacttttcc tcttactcga cttaaccagt accaacccag cttctcaacg gatttatacc
     4381 atggcacttt aaagccagca tcactgacaa tgagcggtgt ggtgttactc ggtagaatgc
     4441 tcgcaaggtc ggctagaaat tggtcatgag ctttctttga acattgctct gaaagcggga
     4501 acgctttctc ataaagagta acagaacgac cgtgtagtgc gactgaagct cgcaatacca
     4561 taagtcgttt ttgctcacga atatcagacc agtcaacaag tacaatgggc atcgtattgc
     4621 ccgaacagat aaagctagca tgccaacggt atacagcgag tcgctctttg tggaggtgac
     4681 gattacctaa caatcggtcg attcgtttga tgttatgttt tgttctcgct ttggttggca
     4741 ggttacggcc aagttcggta agagtgagag ttttacagtc aagtaatgcg tggcaagcca
     4801 acgttaagct gttgagtcgt tttaagtgta att
//
LOCUS       DAJVOY010000232         4680 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVOY010000232 DAJVOY010000000
VERSION     DAJVOY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.235"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..1251)
                     /locus_tag="OQL90_003833"
     CDS             complement(<1..1251)
                     /locus_tag="OQL90_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGCS family amino acid carrier protein"
                     /protein_id="HCS1370441.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMA"
     gene            1530..2483
                     /gene="tal"
                     /locus_tag="OQL90_003834"
     CDS             1530..2483
                     /gene="tal"
                     /locus_tag="OQL90_003834"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1370442.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2598..3185
                     /gene="mog"
                     /locus_tag="OQL90_003835"
     CDS             2598..3185
                     /gene="mog"
                     /locus_tag="OQL90_003835"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1370443.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(3220..3786)
                     /gene="satP"
                     /locus_tag="OQL90_003836"
     CDS             complement(3220..3786)
                     /gene="satP"
                     /locus_tag="OQL90_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1370444.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3935..4648)
                     /gene="msyB"
                     /locus_tag="OQL90_003837"
     CDS             complement(3935..4648)
                     /gene="msyB"
                     /locus_tag="OQL90_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1370445.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
BASE COUNT         1238 a         1213 c         1188 g         1041 t
ORIGIN      
        1 ggccattatg atatctgcca gttgccacat cagcggaagg cttagcaagg tgccgccgat
       61 gaccgttgcg aaggtgcaga tccgcaaaca ccagatcgct ttaggggtgt tcaggcgtaa
      121 aaagaagaga ttgttttcgg cataaatgta gttggcaacg atggagctga aggcaaacag
      181 aataaccacg agggtaacaa actcagcacc ccaggaaccc attagcaccc gcatcgcctt
      241 ctggataagc tgaatacctt ccagcggcat gtaggttgtg tcgttacccg ccagtaatat
      301 cagcatggcg cttgccgtac agatgaccag ggtgtcgata aaaatgccaa tcatctggac
      361 aatcccttgc gctgccggat gcggaggcca ggacgccgct gccgctgccg cgtttggcgt
      421 cgaacccatt cccgcctcat tggaaaacat actgcgctga aaaccgttag taatcgcctg
      481 gcttaaggta tatcccgccg cgccgcctgc cgcttcctgc cagccaaaag cactctcaaa
      541 aatagaccaa atgacgtggg gaagttgccc gatatttatt acgcaaatca ccatgctggt
      601 cagcacccag attatcgcca tcaacgggac aaacccctgc atgagccggg cgacgccatg
      661 aagaccgcga gtgattgcca gcagagcaaa gacagcgaga ataatgcctg tcaccagcgg
      721 gggaaaatca aaagaaaaac tcagggctcg ggcaacggcg ttcgcctgaa ctccgctgaa
      781 aattatgccg taggcgatga gcaaaaagac ggcgaacaga acgcccatcc aacgcatccc
      841 cagcccgcgc gccatatacc atgccggtcc gccacgaaac tgcccattga cgtcacgttc
      901 tttataaagt tgtgcaaggg aacattcggc aaacgaggtc gccatgccga taaacgcggc
      961 aacccacatc caaaagacgg ctctaggtcc accggtggta atagccagcg caacgccggc
     1021 caggttgccg ctacccacgc gcgccgcaag actggtacac aatgactgaa atgaggttaa
     1081 accgcctggc tgtggatgaa tgctattttt aagacttttg ccaaactggc ggatgtagcg
     1141 aaactgcaca aatccggtgc gaaaagtgaa ccaacaacct gcgccgaaga gcaggtaaat
     1201 cattaccgat ccccaaagga cgctgttaat aaaggagaaa aaatctggca tgcatatccc
     1261 tcttattgcc ggtcgcgatg actttcctgt gtaaacgtta ccaattgttt aagaagtata
     1321 tacgctacga ggtacttgat aacttctgcg tagcatacat gaggttttgt ataaaaatgg
     1381 cgggcgatat caacgcagtg tcagaaatcc gaaacagtct cgcctggcga taaccgtctt
     1441 gtcggcggtt gcgctgacgt tgcgtcgtga tatcatcagg gcagaccggt tacatccccc
     1501 taacaagctg tttaaagaga aatactatca tgacggacaa attgacctcc cttcgtcagt
     1561 acaccaccgt agtggccgac actggggaca tcgcggcaat gaagctgtat caaccgcagg
     1621 atgccacaac caacccttct ctcattctta acgcagcgca gattccggaa taccgtaagt
     1681 tgattgatga tgctgtcgcc tgggcgaaac agcagagcaa cgatcgcgcg cagcagatcg
     1741 tggacgcgac cgacaaactg gcagtaaata ttggtctgga aatcctgaaa ctggttccgg
     1801 gtcgtatctc aactgaagtt gatgcgcgtc tttcctatga caccgaagcg tcaattgcga
     1861 aagcaaaacg cctgatcaaa ctctacaacg atgctggtat tagcaacgat cgtattctga
     1921 tcaaactggc ttctacctgg cagggtatcc gtgctgcaga acagctggaa aaagaaggca
     1981 tcaactgtaa cctgaccctg ctgttctcct tcgctcaggc tcgtgcttgt gcggaagcag
     2041 gcgtgttcct gatctcgcca tttgttggcc gtattcttga ctggtacaaa gcgaatactg
     2101 ataagaaaga gtacgctccg gcagaagatc cgggcgtggt ttctgtatct gaaatctacc
     2161 agtactacaa agagcacggt tatgaaaccg tggttatggg cgcaagcttc cgtaacatcg
     2221 gcgaaattct ggaactggca ggctgcgacc gtcttaccat cgcacctaca ctgctgaaag
     2281 agctggctga gagcgaaggg gctatcgaac gtaaactgtc ttacaccggc gaagtgaaag
     2341 cgcgtccggc gcgtatcact gagtccgagt tcctgtggca gcacaaccag gatccaatgg
     2401 cagtagataa actggcggaa ggtatccgta agtttgctat tgaccaggaa aaactggaaa
     2461 aaatgatcgg cgatctgctg taatcattct tagcgtgacc gggaagtcgg tcacgctacc
     2521 tcttctgaag cctgtctgtc actcccttcg cagtgtatca ttctgtttaa cgagactgtt
     2581 taaacggaaa aatcttgatg aatactttac gtattggctt agtttccatc tctgatcgcg
     2641 catccagcgg cgtttatcag gataaaggca tccctgcgct ggaagaatgg ctgacatcgg
     2701 tgctaaccac gccgtttgaa ctggaaaccc gcttaatccc cgatgagcag gcgatcatcg
     2761 agcaaacgtt gtgtgagcta gtggatgaaa tgagttgcca tctggtgctc accacgggcg
     2821 gaactggccc ggcgcgtcgt gacgtaacgc ccgatgcgac gctggcagta gcggaccgcg
     2881 agatgcctgg ctttggtgaa cagatgcgcc agataagcct gcattttgta ccaactgcga
     2941 tcctttcgcg tcaggttggt gtgattcgca aacaggcgct gatccttaac ttacccggtc
     3001 agccgaagtc tattaaagag acgctggaag gtgtgaagga cgctgcgggt aacgttgtgg
     3061 tacacggtat ttttgccagc gtaccgtact gcattcagtt gctggaaggg ccatacgttg
     3121 aaacggcacc agaagtggtt gctgcattca gaccgaagag tgcaagacgc gacgttagcg
     3181 aataaaaaaa tccccccgag cggggggatc tcaaaacaat tagtgggatt caccaatcgg
     3241 cagaacggtg cgaccaaact gctcgttcag tacttcaccc atcgccagat agattgcact
     3301 ggcaccacag atcagaccaa tccagccggc aaagtggatg attgcggcgt taccggcaat
     3361 gttaccgatc gccagcaggg caaacagcac agtcaggcta aagaaaacga attgcagaac
     3421 gcgtgcgcct ttcagcgtgc cgaagaacat aaacagcgta aatacgcccc acagacccag
     3481 gtagacacca aggaactgtg catttggcgc atcggtcaga cccagtttcg gcatcagcag
     3541 aatcgcaacc agcgtcagcc agaaagaacc gtaagaggtg aatgcggtta aaccgaaagt
     3601 gttgcctttt ttgtactcca gcagaccagc aaaaatttgc gcgatgccgc tgtagaaaat
     3661 gcccatggca agaataatac cgtccagagc gaaataaccc acgttgtgca ggttaagcag
     3721 aatggtggtc atgccgaagc ccatcaggcc cagcggtgcc ggattagcca acttagtgtt
     3781 gcccataatt cctcaaaaat catcatcgaa tgaatggtga aataatttcc ctgaataact
     3841 gtagtgtttt cagggcgcgg cataataatc agccagtggg gcagtgtcta cgatcttttg
     3901 aggggaaaat gaaaattttc cccggtttcc ggtatcagac ctgagtggcg ctaaccatcc
     3961 ggcgcaggca ggcgatttgc agtacggctg gaatcgtcac gcgataggcg ctgccgctga
     4021 ccgctttaac cccatttagt gccgcaccta cagggcctcc cagccccgcg ccgcgcagca
     4081 aaccatgccc aagtacgctc attgctgcgt gggtgcgtaa aatgcgggtc agttggctgg
     4141 aaagcaaatg cgacacgcct tttgccaata atttgtcttt catcagcagc ggcagcagct
     4201 cttccagctc attcaccctc gcatcgaacg cgtgcagaaa ctcctgctta tgttcctcgt
     4261 ccattttctt ccaggtatta cgcagaaatt gttccagtaa ctgttgctca atttcaaacg
     4321 tagacatctc tttgtcggct ttcagcttca atcgttttga aacatcgagc aaaatggccc
     4381 gatacaattt accgtgtccg cgcagtttgt tggcgatact atcgccacca aaatgctgta
     4441 attctccagc aatcagctgc cagttgcggc gatgttgctc gggatgccct tccatcgatt
     4501 taaacagttc gttgcgcatc agtacgctgg agaggcgagt tttgcctttt tcattatggg
     4561 tgagcaatcg ggcgaaattt gccaactgtt cctcactaca atgctgaaga aaatccagat
     4621 ctgaatcatt caggtaatta acattcattt tttgtggctt ctatattctg gcgttagtcg
//
LOCUS       DAJVOY010000233         4655 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVOY010000233 DAJVOY010000000
VERSION     DAJVOY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.236"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            50..1849
                     /gene="cysJ"
                     /locus_tag="OQL90_003838"
     CDS             50..1849
                     /gene="cysJ"
                     /locus_tag="OQL90_003838"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1370446.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            1849..3561
                     /gene="cysI"
                     /locus_tag="OQL90_003839"
     CDS             1849..3561
                     /gene="cysI"
                     /locus_tag="OQL90_003839"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1370447.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3636..4370
                     /gene="cysH"
                     /locus_tag="OQL90_003840"
     CDS             3636..4370
                     /gene="cysH"
                     /locus_tag="OQL90_003840"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1370448.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
BASE COUNT         1150 a         1217 c         1328 g          960 t
ORIGIN      
        1 gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca tgacgacaca
       61 ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc ttcaggcggc
      121 cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct ggggcgtact
      181 caatcagcag cctgctgcgc ttgcagcgac gccagcgcca gccgcagaaa tgccgggtat
      241 aactattatc tccgcctcgc aaaccggcaa tgcgcgccgg gttgctgaag cattacgcga
      301 tgatttatta gcagcaaaac tgaacgttaa gctggtgaac gcgggcgact ataaattcaa
      361 acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg aaggggaacc
      421 gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc caaagctgga
      481 aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt tctgccagtc
      541 cgggaaagat ttcgacagca agctggcgga actgggtggt gaacgcctgc tcgaccgtgt
      601 cgatgccgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg tggttgatgc
      661 gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg gcgcggtaaa
      721 tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc tctctgttaa
      781 ccagaaaatt accgggcgta actctgaaaa agacgttcgc catatcgaaa ttgacttagg
      841 tgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc agaacgatcc
      901 ggcactggtg aaagaacttg tcgaactgct gtggctgaaa ggcgatgaac ctgtcaccgt
      961 cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac tgaccgtcaa
     1021 caccgccaac attgttgaga attacgccac gcttacccgc agcgaaacac tgctgccgct
     1081 ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgccgattg tcgacatggt
     1141 gcgtttctct ccggcgcaac tggatgccga agcgctgatt aatctgctgc gcccgctgac
     1201 cccgcgcctg tattccatcg cctcctcgca ggcggaagtc gagaacgaag tacacgtcac
     1261 cgttggtgtg gtgcgttacg acgtggaagg ccgagcccgt gccggtggtg cctccagctt
     1321 cctcgcggat cgcgtggaag aagagggcga agtccgcgta tttatcgaac ataacgataa
     1381 ctttcgcctg cccgctaacc cggaaacccc ggtgattatg attggcccag gcaccggtat
     1441 tgcgccgttc cgcgccttta tgcagcaacg cgccgccgac gaagcgccag gtaaaaactg
     1501 gctgttcttt ggtaatccgc actttacgga agacttcctg taccaggtgg agtggcagcg
     1561 ctacgtcaaa gatggcgtgc tgacacgtat cgatcttgcc tggtcgcgcg accaaaaaga
     1621 aaaagtttac gtacaagaca aactgcgcga acagggcgcg gagctgtggc gctggatcaa
     1681 tgatggtgcc cacatttatg tctgcggcga cgctaatcgc atggcgaaag acgttgagca
     1741 ggcacttctg gaagtgattg ccgaatttgg tggcatggac atcgaagcgg cggatgaatt
     1801 tttaagtgag ctgcgcgtag agcgccgtta tcagcgagat gtctactaat gagcgaaaaa
     1861 catccagggc ctttagtggt cgaaggaaaa ctgacagacg ccgagcgcat gaagcttgaa
     1921 agcaactacc tgcgcggcac cattgcggaa gatttaaacg acggtctgac cggcggcttt
     1981 aagggcgaca acttcctgct gatccgcttc cacggcatgt atcagcagga tgaccgcgac
     2041 atccgcgccg aacgtgctga acagaagctg gagccgcacc acgcgatgct gcttcgctgt
     2101 cgtctgccgg gtggggtgat taccactaaa cagtggcagg cgatcgacaa atttgccggt
     2161 gaaaacacca tctatggcag cattcgcctg accaaccgcc agacattcca gttccacggc
     2221 attctgaaaa agaacgtcaa accggtgcac cagatgctgc actcggtcgg tcttgatgcg
     2281 ctggcgacag ctaacgacat gaaccgtaac gtactctgca cctcgaaccc ttacgagtcg
     2341 cagctgcacg cggaagcgta cgagtgggcg aagaagattt ctgagcatct gttgcctcgt
     2401 acccgcgcgt atgcggagat ctggctcgac caggaaaaag tcgccactac tgatgaagaa
     2461 ccgatccttg gtcagaccta cctgccgcgt aagttcaaaa ccacggtagt gatcccgccg
     2521 cagaacgata tcgatctgca tgccaacgac atgaacttcg tggcaatcgc cgaaaacggc
     2581 aagctggtag gcttcaacct gctggtgggc ggtgggcttt ctatcgaaca cggcaacaag
     2641 aaaacctacg cccgcacggc gagcgagttt ggctatctgc cgctggagca tacgctggcg
     2701 gtggccgaag ctgtcgtgac aactcagcgt gactggggta accgaaccga tcgtaaaaat
     2761 gccaaaacca aatacacgct ggagcgcgtg ggggttgaga cgtttaaagc ggaagtggag
     2821 cgtcgcgcgg ggatcaaatt tgaaccgatc cgtccatatg agttcaccgg acgaggcgat
     2881 cgtattggct gggttaaggg cattgatgat aactggcacc tgacgctgtt tatcgaaaat
     2941 ggtcgcatcc ttgattatcc ggggcgtccg ctgaaaaccg gcctgctgga gatcgcgaag
     3001 atccacaaag gcgatttccg cattacggcg aaccagaatc tgatcatcgc cggtgtaccg
     3061 gaaagcgaga aagcgaagat cgagaagatc gccaaagaga gcgggttaat gaatgccgtc
     3121 acgccgcagc gtgaaaactc gatggcctgc gtgtcattcc cgacttgccc gctggcgatg
     3181 gcggaagcag agcgtttcct gccgtctttt atcgacaaca tcgataattt aatggcgaaa
     3241 catggtgtca gcgatgagca tatcgtgatg cgtgtaacag gctgcccgaa cggttgtggt
     3301 cgcgcgatgc tggcggaagt gggcctggtg ggtaaagcgc cgggtcgcta caacctgcat
     3361 cttggcggca accgcattgg gacacgtatc ccacggatgt ataaagaaaa catcaccgag
     3421 ccggaaatcc tggcgtcgct tgatgaactg atagggcgct gggcgaaaga gcgcgaagcg
     3481 ggtgaaggct tcggcgactt tacggtgcgt gcgggcatca ttcgcccggt gctcgatccg
     3541 gcgcgcgatt tgtgggatta accatcagcc cggtcttgta ggcctgataa gaacgcgtga
     3601 gcgtcgcatc aggcaaggca aacagtgagg aatctatgtc caaactcgat ctaaacgccc
     3661 tgaacgaact gccgaaggta gatcgcattc tggcgctggc ggaaactaac gccgaactgg
     3721 aaaaactgga cgctgaaggc cgcgtagcct gggcgctgga taatctgccc ggtgaatatg
     3781 tactttcttc cagctttggc attcaggcgg cggtgagcct gcatctggtg aatcaaattc
     3841 gcccggatat tccggtgatc ctcaccgata cgggttactt gttcccggaa acctaccgct
     3901 ttattgacga gttaacggac aaactcaagc tcaacctgaa agtgtaccgt gctaccgaaa
     3961 gcgcagcctg gcaggaagca cgctacggaa aactgtggga gcagggcgtt gaaggcattg
     4021 aaaagtacaa tgacatcaac aaagtcgaac cgatgaaccg ggctctgaaa gaacttaacg
     4081 cgcaaacctg gtttgctggc ctgcgccgtg aacaatccgg cagtcgcgcc aatttaccgg
     4141 tgctggcaat tcagcgtggc gtatttaaag tgctgccgat tatcgactgg gataaccgaa
     4201 ctatttatca gtacctgcaa aaacatggcc tgaaatatca cccattatgg gatgaaggat
     4261 atttatcggt cggtgatacc catacaaccc gtaaatggga acctggcatg tcggaagaag
     4321 aaacacgttt ctttggctta aaaagggaat gtggattgca cgaagggtaa atattgccgg
     4381 ataaattaaa cgcctctgtc agaaatgatg ggggcgtttt tattttgcga gcagtattcc
     4441 tgattttaaa cgtgaacgcc gctggaagaa ttatcaggta tagaatggcg tcgggtgctt
     4501 gaggctgtct ggcgttaagc attcgcgagg ttccagatgg acaaaagccc caggcgatat
     4561 ttctatcaac ctgaggccag cgttcgaacc caaacaattc gaatgttagt ctcttctttg
     4621 cagggaggca agacatgctg acaaaatatg ccctt
//
LOCUS       DAJVOY010000234         4652 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVOY010000234 DAJVOY010000000
VERSION     DAJVOY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.237"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..745)
                     /locus_tag="OQL90_003841"
     CDS             complement(<1..745)
                     /locus_tag="OQL90_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCS1370449.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLA"
     gene            complement(876..2240)
                     /gene="sdaA"
                     /locus_tag="OQL90_003842"
     CDS             complement(876..2240)
                     /gene="sdaA"
                     /locus_tag="OQL90_003842"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1370450.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2424..3002)
                     /locus_tag="OQL90_003843"
     CDS             complement(2424..3002)
                     /locus_tag="OQL90_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1370451.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3006..4367)
                     /gene="pabB"
                     /locus_tag="OQL90_003844"
     CDS             complement(3006..4367)
                     /gene="pabB"
                     /locus_tag="OQL90_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1370452.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4441..4620
                     /locus_tag="OQL90_003845"
     CDS             4441..4620
                     /locus_tag="OQL90_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1370453.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1168 a         1196 c         1209 g         1079 t
ORIGIN      
        1 ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc cccggaccat
       61 taacgttaat ggtaaaagga aagcgttgcg aagaaacctg atagttttca ttgtcttcag
      121 gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg tccaccgtca
      181 ggctggcgct actgatttgc tgcagttgtg gctcaaggag cattgccgtc agtaagtcga
      241 tgttaatcat ctccatgacc ccagcattgc tactgcccgc tgcaggcgtc cattgaatca
      301 aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt ggttgcggtg
      361 caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa atgctggagc
      421 aatagagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt tttgccgcct
      481 gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga agcaagactt
      541 cgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg tgattggcga
      601 attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg ctcagggtcg
      661 aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgt cgataggttt
      721 taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc gacaaacgcc
      781 tgacgcaaca gtggatgtgt agtggggaat attacagcag acgagaaagc gggtataaat
      841 tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac tttgattgcc
      901 agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt ttcgtacatc
      961 gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag agccatccgt
     1021 gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg cacctgaacc
     1081 tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat ttccgccgcc
     1141 acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc agccattgaa
     1201 caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga ggcgttcatt
     1261 ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat gtccgggctg
     1321 accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc gcaggcaccg
     1381 ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac tgccagcgca
     1441 aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc gctggaaacc
     1501 agcatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg cagtacacct
     1561 tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg cgcgaaatac
     1621 tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag accagacagc
     1681 gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa cggatacggc
     1741 acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat aaaaccgccg
     1801 ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc gtggatttgc
     1861 ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt gtcgcgcggg
     1921 aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttc tacgtcgcga
     1981 ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc aagacccata
     2041 ataatggcaa tatcggtgtg gtggccttta cccgtcagcg acagtgaacc ataaacgtcc
     2101 acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc gacgaactgt
     2161 ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc caccttaaac
     2221 atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt ccagtaacga
     2281 actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta ttcacatgaa
     2341 ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca agtagtgttt
     2401 tgtagatgta aagataagta tagtcagggt ttcacaccaa tttgcagcgc cagctcacga
     2461 attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag ccatacccga
     2521 tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg taatgcctgg
     2581 gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata cggcagatcg
     2641 ggcgggataa tgccgatcac tggggttacc tgatagccag ttacgctatc gacgggcggc
     2701 agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc agcttcgcgc
     2761 agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg gaatgccact
     2821 tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc cggttgcggt
     2881 cgacggacga tggggattaa cacggcagcc tgacgatgat ttagggtttc ccggttaatt
     2941 tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct acggtattcc
     3001 acgtcttact tctccagttg gcgtaatatc ttagtaactt tatcaaaagt ttcctgatat
     3061 tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta tatttgtccg
     3121 ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca aaagctcaaa
     3181 tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc gataatttcc
     3241 atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc gcgcagcaga
     3301 tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag atgatgcacg
     3361 gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc tacggcaaca
     3421 cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg atctttcgct
     3481 gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag gcgtggtagc
     3541 gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa ccgctctggc
     3601 gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg cgcgcggttg
     3661 gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc atgaaaacgc
     3721 tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac ctggcgaaat
     3781 ttttcgccgt actgctcgcg ggtcatattg gattgccagt cggaagtgag cgtgaaatct
     3841 tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac atcattatga
     3901 ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc gtagataccc
     3961 actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga ctcaaaacgg
     4021 cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa atcttcgtta
     4081 tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag cgggtcatca
     4141 gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttaccgaa agtggttaaa
     4201 gtgcaaatcg gctcggcgac cacaatatca aagcggctat acggatgatc ggcatagccg
     4261 gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa ttcagcggcg
     4321 tcctgacgcc agggtaaagt aatcacagcg ggagataacg tcttcatcag tcctgactct
     4381 actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca ggagttaaca
     4441 atgtttgcag gtttaccttc actcacccat gaacagcagc aaaaagctgt cgagcggatc
     4501 caggaactga tggcacaagg gatgagcagc ggccaggcaa tcgcgctggt ggcagaagag
     4561 ctgcgtgcca accactccgg cgagcgaatc gtggcgcgtt ttgaggatga agacgagtaa
     4621 cacactaatc cgtatcgatt tcgttataga ag
//
LOCUS       DAJVOY010000235         4540 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVOY010000235 DAJVOY010000000
VERSION     DAJVOY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.238"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            91..840
                     /locus_tag="OQL90_003846"
     CDS             91..840
                     /locus_tag="OQL90_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1370454.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARWILTEETIL"
     gene            1016..1513
                     /gene="rayT"
                     /locus_tag="OQL90_003847"
     CDS             1016..1513
                     /gene="rayT"
                     /locus_tag="OQL90_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS1370455.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(1737..3449)
                     /locus_tag="OQL90_003848"
                     /pseudo
     CDS             complement(1737..3449)
                     /locus_tag="OQL90_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            3421..4206
                     /gene="lafU"
                     /locus_tag="OQL90_003849"
     CDS             3421..4206
                     /gene="lafU"
                     /locus_tag="OQL90_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS1370456.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHIDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
BASE COUNT         1078 a         1198 c         1188 g         1076 t
ORIGIN      
        1 atagtaatat gcaaataagc aattaataat cccgtttact cctccggtct tttaaaattc
       61 tcaccttcat tcagccggtt tatgtccttc atgtcttcgt tcttgctcgg cagatttctg
      121 catccaggcg tgttttcgtt atgcgttttg ctccctttac tcgccagcgc caccacatca
      181 catatctctt tcagctacgc cgcccgccag cggatgcaaa accgtgcgcg tttattaaaa
      241 cagtaccaaa cccatctgaa aaaacaggcc agctacattg tggaaggcaa tgccgaaagc
      301 agaagggcgc tacgccagca caaccgggag cagataaaac agcatccaga atggtttcct
      361 gcgccgctca aggcaagtga cagacgctgg caggcgttgg tagaaaacaa tcatttcctc
      421 agcagcaacc acctgcataa tatcaccgaa gtggcgattc accgtctgga gcaacagctt
      481 ggcaagcctt acgtctgggg cggcacgcgg cccgatcaag gcttcgactg tagcgggttg
      541 gttttttatg cctacaacaa gatccttgag gctaagctcc cgcgcacggc taatgagatg
      601 tatcactacc atcgggcaac cattgtggca aataacgacc tgcgccgggg agatttgctg
      661 tttttccata tccacagccg tgaaattgcc gaccatatgg gcgtgtatct gggagacggt
      721 cagtttattg agtcgccgcg cacgggggaa accattcggg taagtcggtt agctgaacct
      781 ttctggcagg atcatttttt gggcgcgcgg tggatattga cggaagagac gattttgtag
      841 gacggataag gcgtttacgc cgcatccggc ggtggtgcgc agatgcctga tgcgacgctg
      901 acgcgtctta tcatgcctac aaatctgtgc gtttttgcga gccgctttcc tgttattcca
      961 tcacctcatt gccgaccttt ccttttcttc ttaccgttga gaaaaaggag tcgccatgtc
     1021 tgaatatcgt cgttattaca tcaagggggg aacctggttt ttcacggtga atttacgcaa
     1081 tcgtcgaagc caactcttga ccacccagta ccagatgctc cgtaacgcca ttattaaagt
     1141 taagcgagac aggccttttg aaatcaacgc ctgggtcgtt ttgccagagc atatgcattg
     1201 catatggaca ttacctgaag gcgatgatga tttttcctct cgctggcggg aaattaaaaa
     1261 gcaatttacc catgcttgtg gattgaaaaa tatctggcaa ccacgttttt gggagcacgc
     1321 catccgcaat accaaagatt ataggcatca tgttgattat atttatataa atccagtaaa
     1381 gcatggttgg gtaaagcaag tgagtgattg gccattctca acgttccatc gcgatgtcgc
     1441 gcgagggtta tatcccatcg attgggcggg ggacgtaacg gatataaatg ctggcgagcg
     1501 tatcatttta taattatgcg cagatgcctg atgcgacgct ggcgcgtctt atcatgccta
     1561 caaacctgtg ccggatcggt aggccggata aggcgtttat gccgcatccg gcggttatgc
     1621 gcagatgcct gatgcgacgc tggcgcgtct tatcatgcct acaaacctgt gctggatcgg
     1681 taggccggat aaggcgttca cgccgcatcc ggcagttgtg caccaatgct ggatacttac
     1741 atcaacgccc ccataatctt caactccagc tcgtccggca cttcgttata cgacagcaca
     1801 tgcagccccg gcgcaaacaa ccttgcataa cgcgccagca aagggcgcag ctgcggtggc
     1861 accagcagca ccgggtcttt ccccgccgct ttcatctgct ctttcacctg tggcatcgta
     1921 ctctggaact ggttgagcat attcgggtcc actggcacgc tgtcgagcat cactttaccg
     1981 ccctgttgcg cctgattcac taggttagtc agcagatttt ccagctcatt attcagggta
     2041 tacaccgtca gctcctgctt gcgaacgaac ggatgggtaa tgctgcgccg cagcgccagg
     2101 cgcacatcgg ctgccagcag aatatgatct ttggtcaccg cgctactggc gaccagcacg
     2161 gtggcgatgg tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc ccggtacact
     2221 ttcagcaact ggctgtaatt gagcaccgcg cttaaatcct ccgccaggcg cggtgccatc
     2281 gacgacaaac ggttatgcaa ctgcgtaata tcgtcatagt taaataaatc aggaatatag
     2341 ctgcgcacaa tcttatttac atgggtagca atcacgctgg cgctgtcgat cacctgatac
     2401 cccatattca gcaccttcgc tttctgcgca ggctggatcc aggtcaccgg catcccgtac
     2461 gccgggtcgt tccccagcac gccgtctatc tcgccgtagg tttcgctgga gggctgggcc
     2521 atcagtttat ccgccggaat atccgcttca tcagccttaa tgccgttgat gaaaatagcg
     2581 tactggctgg gcttaaggcg gaagttttcc cgaatgcgga tctccggcag cagcacgccg
     2641 ttgccgtcgg atatcacctg tcgcacgccg cgaatacgct gggtgagcgg gttgccctgg
     2701 gctttatcga ccagcgccac cagcttgtag ccgaggctta aactgatagg ctcgataagc
     2761 ggaatggttt cccaactgac ctgttgctcg ctggtttcgg tgatggtgcg ggtcagcgtt
     2821 tcgagacttt tctcttccgc ttccgccgcc tgcgggcgtt tgctcatccg ccagccggta
     2881 aagccaagca gggcgctgaa cagcaaaaac ggcagatgtg gcattcccgg cactaccgcc
     2941 agtacgaaca taattccggt agcggtgtag agcaccgatg ggcttgccag caactggtta
     3001 cgcacgtcat gggcgatatc gccgttgtcg ctgacgcggg tgaggataat cgccgccgcg
     3061 gtggagagta gcagggaagg gatttgcgcc accagcccgt cgccgatggt catcagcaca
     3121 tactgctgga aggcggcgtc ggcgctcagg ttgtatttga agatcccgat acagacgccg
     3181 ccgatcaggt tgatcgccag aatcatcatc ccggcgatag catccccgcg cacaaatttc
     3241 gatgccccgt ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt acgtcgggtt
     3301 tgtgcctgcg cctgattgat caacccggca ttaagatcgg cgtcaatcgc catctgctta
     3361 ccgggcatcg catccagggt aaagcgggct gaaacctcgg aaatacgctc ggcccctttg
     3421 gtgacgacga taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc
     3481 gccgcgctgc acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc
     3541 agcccgtctc atccgccaaa acctgccacc gtggcggtgc cggaagaaac ggagaaaaaa
     3601 gcgcgcgacg tcaatgagaa aacggcgctg ctgaagaaga aatccgccac cgagcttggc
     3661 gagctggcaa ccagtatcac caccattgcc cgtgatgcgc atatggaagc gaatctggag
     3721 atggagattg ttccccaggg attacgcgtg ctgattaaag acgaccagaa tcgcaatatg
     3781 ttcgaacgcg gcagcgcgca gattatgccg ttctttaaaa cgctgctggt ggagctcgcg
     3841 ccagttttcg actcgctcga taacaaaatt attattaccg ggcatatcga tgcgatggct
     3901 tacaaaaaca atatctacaa caactggaac ctttcgggtg accgcgcgct ttcggctcgt
     3961 cgggtgctgg aagaggccgg aatgccggaa gataaagtga tgcaggtaag cgcaatggcg
     4021 gaccagatgc tgctggattc caaaaatccg caaagcgcgg gcaaccggcg cattgagatt
     4081 atggtgctga ccaaaagtgc gtccgatacg ctgtatcaat actttggtca gcatggggat
     4141 aaagtggtgc agccgctggt gcaaaagctg gataagcagc aggtgctttc gcagcgaacg
     4201 cgttaaatgc tgaatcttta cgcatttctc aaactctgaa atcactgtat actttaccag
     4261 tgttgagagg tgagcaatgc gtaaaatcat gattttcact tcctaaaaca ccccttataa
     4321 tctggtcata gagtcattat agcactggat gaatatacag gtgaggaagg tttacagtta
     4381 cattcgcttt tcgtcaacga aacaagcttt tggtgatagt catcgaagac agagtaaggc
     4441 catacaggat tggttagcca gccatccaga ccacatccta gacgaatctc ttagttttga
     4501 agatttagga cgttcagcct ttcacggtga ccatctaaaa
//
LOCUS       DAJVOY010000236         4524 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVOY010000236 DAJVOY010000000
VERSION     DAJVOY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.239"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..594)
                     /locus_tag="OQL90_003850"
     CDS             complement(<1..594)
                     /locus_tag="OQL90_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1370457.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWFNSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLY"
     gene            complement(587..2866)
                     /locus_tag="OQL90_003851"
     CDS             complement(587..2866)
                     /locus_tag="OQL90_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370458.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQRNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLANAVTGCYLTPYSEKHLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASIADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAVMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(2877..3965)
                     /locus_tag="OQL90_003852"
     CDS             complement(2877..3965)
                     /locus_tag="OQL90_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCS1370459.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1052 a         1197 c         1235 g         1040 t
ORIGIN      
        1 atacaacttg ccgtgttgcg ggtgccagtg ttgcaggttg gcgcgcagag tacttttgaa
       61 atcaaagttt cgcgccagtg gaataaatga gcggcgacgg cgatcgcgga caccagaaaa
      121 agcctgacga acttcctttg ccagtcgcgc cataatttct tcaacaacct ggcgcacaat
      181 cctgcgggcg gcagccagta cttcggggtt catcagatgt ttggtgtgca aaacggcgcg
      241 tagcaggctt tcggaaggct gcatacgttc cagcacgtcg agatttgtca ccacatcttc
      301 aatgccgtag cgcagtacgg catcgctttc cagccgctca atcacctgtt gcggaaacag
      361 cgtgtggata ctgttgaacc actcaggagt ggtgagattt gagccaccta atccaccaga
      421 gcgttcacca cgctggagcc gttcaggatc gcgcccatac agccactcca gcgcgtgatc
      481 tatctgccgg gcgttgtcat ccagcccaca aagcgtcgtt tctgccgctt cgccaagaat
      541 taatcgccag cgttgtagct cacgggtggt cagaagatcg ttcagttcag acatggaaaa
      601 ctccccagtg ttgcagcgat gccagtgcct gctgttcgag ttgttgatga tgtgcaatgg
      661 cttgcggtgg acaatgcaac ggcatttgca gtgccgaaac gggaagttgc gccagttgat
      721 aatgctcaag cacctgatgc gccagcgtcc cgcgttctcg tggcggtagc caggccatca
      781 ccgcgcgtaa atcgggcagg gcattgataa aattggcatc actcagttga ttcaaatggc
      841 tgctgaaacc ggcgataaat gcaggttgac aggccagttg atgacgggct aatgccagca
      901 aaccgtgcag cgcctcgccg gattgtgccg gggataactg cgccagcatc gtcagcgccg
      961 cttcggcact ggcgttggga tgttccagac gcattagtgc gccaagagcc gcgccgcgat
     1021 ccagcgccgg ggcatgaatc gcctgactgc gtcgctccag taaggctacc gccgcagaaa
     1081 gcgaaacgcc ggataaatta acgccgctat gtagatcgcg cagaatatgg caaagcgctt
     1141 gccagctatg caggtgagcg tgaaactcct tttcatccgg tctgccgtta gattcacaca
     1201 gccacagcgt gcgatcgata gccgcgcata acgtcgtttg taatatctgc gcgccttgca
     1261 taccgctaat ttcatccagc cgccataagg catatagcac ttccagcgcc tggcccattt
     1321 cggcaaattg attttcctgg gcgattaata atgtgagttg ctccagtaat tgttgactga
     1381 aggacgccag acccgctaac gccgcctggc taagacatgt ggtgatactg ccgatgccac
     1441 ccgcgtccag catatcggct tctaatttat ggcgtgcggc ttcctgaagt gttgcaccaa
     1501 agcatgcggc ctcaatcaat gccgcatgct ggctaagcgg gcgggttaat ttccagtgtt
     1561 cttcaccgtt gcctgcaagt gtcagtgtac ttccctgctg gcgtacaatc ccagggatct
     1621 ccagtattgc cagccgatgt aacacctgac tttgcgccag cccatcgggg gtaaagcgat
     1681 ttaaggtaag ctccgccgga agagagattg cggtacgttc cagttcgcag gtgacatctt
     1741 ttggcagagg cggttgtggc gtggaagggg caagtttacc gaatccgtca cccgctaatg
     1801 tgtctatcaa cgttagcaga atcggatcgg tatcgggatg aataacgccg cgatagctcc
     1861 acggcaacgg tgcattcagg gcttctttaa tcagcgagcc tgctatggca tccagccagt
     1921 cagtgcgtaa cggtagtgta tgaccgcgca actgtgccag tgccattgca tgcagatgtg
     1981 cggcagccat atccgcgatc gaagcaggca agttgtgctg gcgcaaacgg gtgagaaccg
     2041 tttttagcag ttgttcaccg gcctgctgta agccccactg ccagcaccag ttttgccaga
     2101 ccggggcagg cataccggaa aggtatcctg ccagcacatc aaggtgcttt tcactgtagg
     2161 gggtgagata acaacctgta acggcatttg ccagcgaggg caattctggc ttgttaattt
     2221 cctgcggaca ttcgcgccac atttttgcca gtgcaggagc gtgccagcca ccgcagacga
     2281 ctaacacgtc gccattattt cgctgcaccg cccatgcaat ccagcgagcc ataaaggctt
     2341 cgcgctgacg attgagtgca tcgccgggga aatcgccccg cagttgggca aaatagtgcg
     2401 ccagcgcaga gggtaatgca atttgctggc tttcatcttc gaacaagtga tcccacaggg
     2461 tgtcgctgtt atccatgcgg gtggcacgca gcagcaaggc ctggctttcc tcttgcgtat
     2521 caggcgaatc gtccacttct tcactctgcg cccagcaagg caaatcgatg aagtaagttt
     2581 gtgcctgaat acgacgtgcg gcttgtagcg cctgccactc cggcgaaaat tcagcaaatg
     2641 gcgtccaggc accacgcccg ggggctgcac cgtcctgata ctggcaataa ctgtaaatag
     2701 ctaccggaag ctggtgggct aaaaacagtt cgtctacccg gtcattaaaa tcagccgggc
     2761 cttcaatcaa tacgtatcgt ggccgctggc tttcgattaa cgatttcacc agccgggcgc
     2821 aggccggact atgatgccgg atgccgacaa ttaacggctc gctcatgcat ctttcctcac
     2881 ggcaggcggt ggcgggcttg ataataagcc tgccagtgag cttctttatg tgtggcgact
     2941 cgttgttcga agtaacggcg cagacgagcg cgatcttctt cgttatcttt gacgatggtt
     3001 ccggcaatgc agtccaccag gtcggctggc tcacctgcgc gattcgctaa aaaccaggcg
     3061 cggacgccta cggcgtgggc cacgttcacg gcttcagcgg tggacataat cgctgttagt
     3121 gtatccattg acgttttctt ttcgccgttg gcacgcagat cgcggaaggt gcggaccagt
     3181 aactccagta ccgcgtcagg cactttatgc ggaataccgc tatgagccag caaacgcgcc
     3241 gaagcactgg cgaccagttc cagctcctgg gcgaaatcca taatcggaaa gacagtttca
     3301 aaatcgaagc gacgctttag cgcagcactc atttcattga cgccgcgatc gcgggtattg
     3361 gcagtggcaa taatattaaa gccttctcgc gcatagagct gactggcttc accagtgagt
     3421 tccggcaccg tcatcaccct gtcagaaagc atacctaaca gacagtcttg cacttccagc
     3481 ggtgtacgcg ttatctcttc aaaacggacg attttgccgt cgcgcattcc ctggtaaagc
     3541 ggggcaggga ccagcgcttc cgttgatgga ccatgattga taagcagggc gtagttccag
     3601 ccgtacttga tttgatcttc ggtagtagat gcgccgccct ggatggttaa cccggcatcg
     3661 ccgctaatag cggttgccag taattcagac aggagagatt ttgcggtgcc aggttccccc
     3721 accaacatca aaccgcgtcc ggtggcaaga gtgactaaca tacgttcgac agcggaggga
     3781 ctaatcacca ctttacggct aatattttgc gcgctatcgc caaggataaa agcacgggcc
     3841 gcaggcaaac ttaactgcca accgggcggg caaggtgcgt tatcattttg ttttaatttt
     3901 gccagttcat cggcgtataa cacagcagca ggcggacgct gcagatgatt attctgcggt
     3961 gacatatatt catccctgaa atggattaat gaaataaacg atattttacg cgcaataaat
     4021 gtttggcgca ttagttgttt gagcgaaaaa tatctggaaa gcagatcaca gtaaataaaa
     4081 aaacctgctg gtgaggcagg ttcttttatt tactactttc aggcctaaca ccacagcctg
     4141 tgcaacatcg tgttccaacg aacgaattat aaccacggct ggaccccgtt gcaattaaga
     4201 taatgagatt tataatccag accgttggga cgttgcgccg gatcgaatct atttcaagtc
     4261 ctggctaccg gacgggtggt gctgcaggtg gtgccctgat ccggagtgca acatcgtggc
     4321 ccaacatacg taaaccagta tcgacaggca gtgtaagggt aaggtgctgg tgttgcgctc
     4381 cttaataagg agagcggatg gacacgctga cacaaaagtt aaccgtgctc attgctgtac
     4441 tggagctatt agtggctctg ttacggttga ttgatttgtt gaagtaacgg gcaacttgaa
     4501 acgcatctaa gggggaatac agtt
//
LOCUS       DAJVOY010000237         4495 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVOY010000237 DAJVOY010000000
VERSION     DAJVOY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.240"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..526
                     /locus_tag="OQL90_003853"
     CDS             <1..526
                     /locus_tag="OQL90_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1370460.1"
                     /translation="LPWLSEADRRLQVQSDLPWWLVCRGAIHKFRCVPHLTGRRFEHG
                     VTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEATGLYQVPLSSAQPGDV
                     LLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHREWHASAF
                     TGICNDLAAASTFV"
     gene            463..1065
                     /locus_tag="OQL90_003854"
     CDS             463..1065
                     /locus_tag="OQL90_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1370461.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1126..>4495
                     /locus_tag="OQL90_003855"
     CDS             1126..>4495
                     /locus_tag="OQL90_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1370462.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFSVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMVWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCSLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTL"
BASE COUNT         1019 a         1117 c         1425 g          934 t
ORIGIN      
        1 tctgccctgg ctgagtgagg ctgaccggcg gctgcaggtg cagagtgatt tgccgtggtg
       61 gctggtctgc cggggggcga ttcacaagtt ccgctgtgtg ccgcatctca ccgggcggcg
      121 ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcttatc atctggcggg
      181 gattgagatg ccggattttc atcgcgggga tgactggtgg cgtcacggtc agaatctcta
      241 tctggataat ctggaggcca cagggctgta tcaggtgccg ttgtcatcag cacaaccggg
      301 cgatgtgctg ctgtgctgtt ttggttcatc ggtgccgaat catgccgcca tttactgcgg
      361 cgacggtgag ctgctgcacc atattcctga acaactgagt aaacgagaga ggtataccga
      421 caaatggcag cgacgcacac actccctctg gcgtcaccgg gaatggcacg catctgcctt
      481 tacggggatt tgcaacgatt tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa
      541 gccatccgcg cactggccac acagctcccg gcgtttcgtc agaaactgag tgacggctgg
      601 tatcaggtgc gcattgccgg gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat
      661 gagccgctgg caaatggtgc agtgatccac atagtaccgc gtctggtggg agctaaaagt
      721 ggcggtgtgt ttcaggcggt gctgggggca gctgttatgg cggttgctat atggatgccg
      781 ggggtaggaa ttatggcgag taatctgctg ttttctctcg gtgccagtat gacgcttggc
      841 ggtgttgcac agatgctggc accgaaagcc agaactcccc gtacacagac aacggataac
      901 ggcaaacaga acacctgttt ctcctcactg gataacatgg ttgcccaggg caatgttctg
      961 cctgttctgt acggtgaaat gcgcgtgggg tcgcgggtgg tatctcagga gatcagcacg
     1021 gcagacgaag gggatggtgg tcaggttgtg gtgattggtc gctgatgcaa aatgtttcat
     1081 gtgaaaccgc ctgcgggcgg ttttgtcgtt tatggagcgt gaggaatggg taaaggcagc
     1141 agtaaggggc ataccccgcg cgaagcgaag gacaacctga aatccacgca gttgctgagt
     1201 gtgattgatg ccatcagcga agggccggtt gacggtccgg tggatggatt aaaaagcgtg
     1261 ctgctgaacg gtacgccagt cctggacagc gaggggaaga caaacttttc cggtgttacg
     1321 gtggtgttcc gcgccggcga gcaggagcag acaccgccgg aggggtttga atcttccggc
     1381 tcagagactg tgctgggtac ggaagtgaaa tacgacacgc cgatcacccg gaccatcacg
     1441 tcggcaaaca ttgatcgtct gcgcctgacc ttcagtgtgc aggcactggt ggaaaacacc
     1501 tcaaaggggg accggaatcc gtcggaagtc cgcctgctgg ttcagataca gcgtaacggt
     1561 ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacttcgca gtatctggcc
     1621 tcggtggtgg tgggtaacct gccgccgcgc ccgtttaata tccggatgcg caggatgaca
     1681 ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcgta caccgaaatc
     1741 atcgatgtga aacagtgcta cccgaacacg gcgctggtcg gcgtgcaggt ggattcagag
     1801 cagttcggta accagcaggt gagtcgcaat tatcatcttc gcgggcgcat tctgcaggtg
     1861 ccgtcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg aacgcttaag
     1921 ccagcataca gcaacaacat ggtctggtgt cggtgggaca tgctcactca tccgcgctac
     1981 ggcatgggga aacgtcttgg tgcggcagat gtggacaaat gggcgctgta tgtcatcggc
     2041 cagtactgcg accagtcagt gccggacgga tttggcggca cggagccgcg catcacctgt
     2101 aacgcttacc tgaccacaca gcgtaaggcg tgggatgttc tcagtgattt ctgctcggcg
     2161 atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga ccgaccgtcg
     2221 gataaagtgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc
     2281 cgctacagct tcagcgccct gaaggaccgc cataatgtcg ttgaggtgaa ctggattgat
     2341 ccggacaacg gtcatgagac ggcgacagag cttgtggagg acgcgcaggc cattctccgt
     2401 tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagccgggg acaggcgcac
     2461 cgcgccgggc tgtggctgat taaaacggaa ctgctggaaa cgcagaccgt ggacttcagc
     2521 gtcggcgcag aagggcttcg ccatgtaccg ggtgatgtca ttgaaatctg cgatgatgat
     2581 tatgccggta tcagcaccgg tggtcgcgta ctggcggtga acagccagac ccggacgctg
     2641 acgctcgacc gtgaaatcac gctgccgtcc tcctgtacca cgctgataag cctggttgac
     2701 ggaaatggca atccggtcag cgtggaggtt cagtccgtca ccgacggcgt gaaggtgaaa
     2761 gtgagccggg ttcctgacgg cgttgcagaa tacagcgtgt gggggctgaa gctgccgacg
     2821 ctgcgccagc gcctgttccg ctgcgtgagt atccgtgaga acgacgacgg cacgtatgcc
     2881 atcaccgccg tgcagcatgt gccggaaaaa gaggccatcg tggataacgg ggcacacttt
     2941 gacggtgacc ggcgcggcac ggtgaatggt gtcacgccgc cagcggtgca gcatctgacc
     3001 gccgaagtca ccgcagacag cggggaatat caggtgctgg cgcgatggga cacgccgaag
     3061 gtggtgaagg gtgtgagctt cctgcttcgc ctgaccgtgg cagcggacga cggcagtgag
     3121 cggctggtca gcacagccag gacgacggaa accacatacc gcttcaggca gctggcgctg
     3181 gggaattaca gtctgacagt ccgggcggta aatgcccggg ggcagcaggg cgatccggcg
     3241 tcggtatcgt tccggattgc ggcaccggca gcgcctgtca ctattgaact gataccgggg
     3301 tattttcaga taacggcggt cccgaaactg gctgtatacg acccgacggt gcagtttgag
     3361 ttctggtttt cggaaaagcg gattgcggat atcaggcagg ttgaaaccag cgcccgctat
     3421 cttggcacgg cgctgtactg gatagccgcc agtatcaata tcaggccggg ccatgattat
     3481 tatttttacg ttcgcagtgt gaacaccgtt ggcaaatcgg catttgtgga ggccgtcggt
     3541 cgggcgagcg atgatgcgga aggttatctg agtttttata aagggttgat caataacacg
     3601 catctcggca aggagttgtg gacgcagatt gataacggtc agcttgcgcc ggacctgact
     3661 gaaatcagga cgtccattac gaatgtcagc aatgaaatca cgcaaaccgt caataaaaaa
     3721 ctggaaaatc agagtgctgc aatccagcag atacagaaag ttcaggttga tacaaataat
     3781 aacctgaaca gcatgtgggc tgtgaagctg caacagatga aggacggacg cctttatatt
     3841 gcgggtatcg gtgccggtat tgagaatacg ccagcaggaa tgcagagtca ggtgctgctg
     3901 gcggcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc gatgtttgtt
     3961 ggtcagggcg atcagatatt catgaacgac gtgttcctga aacgcctgac ggctccgacc
     4021 attaccagcg gcggtaatcc tccggcattt tccctgacac ctggcggacg gctgacggcg
     4081 aaaaatgccg atatcagcgg taacgtgaac gcgaactccg ggacgctcaa caacgtcacg
     4141 attaataaga actgtcgggt tctgggaaaa ttgtccgcga accagattga aggcgatctc
     4201 gttaaaacag tgggcaaacc tttcccacgg gactcccggg caccggagag gtggccatca
     4261 gggaccatta ccgtcagggt ttatgacgat cagccgtttg atcggcaaat tgttattccc
     4321 gcggtggcgt ttcgcggtgc taaacatgag cggaagaata acaatattta ttcgtcatgc
     4381 agcctgatag tgaagaaaaa cggtgctgaa atttataacc gaacgaccct ggataatacg
     4441 ctgatatata cgggtgttat tgatatgcct gccggtcacg gtcacatgac gctgg
//
LOCUS       DAJVOY010000238         4418 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVOY010000238 DAJVOY010000000
VERSION     DAJVOY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.241"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            117..446
                     /locus_tag="OQL90_003856"
     CDS             117..446
                     /locus_tag="OQL90_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1370463.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            694..939
                     /gene="nrdH"
                     /locus_tag="OQL90_003857"
     CDS             694..939
                     /gene="nrdH"
                     /locus_tag="OQL90_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1370464.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            936..1346
                     /gene="nrdI"
                     /locus_tag="OQL90_003858"
     CDS             936..1346
                     /gene="nrdI"
                     /locus_tag="OQL90_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCS1370465.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1319..3463
                     /gene="nrdE"
                     /locus_tag="OQL90_003859"
     CDS             1319..3463
                     /gene="nrdE"
                     /locus_tag="OQL90_003859"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1370466.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENVQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3473..4387
                     /gene="nrdF"
                     /locus_tag="OQL90_003860"
     CDS             3473..4387
                     /gene="nrdF"
                     /locus_tag="OQL90_003860"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1370467.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYSDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSRIGN"
BASE COUNT         1092 a         1126 c         1128 g         1072 t
ORIGIN      
        1 ttacctgatt gcgcattcac aacgctttca gaaaagtcca taaagccgcg atgaacctca
       61 gttaagaaaa ttatggtcta cactgaaaat tacatcgaat tctaatgggg gatcatatgt
      121 ttaaccgacc gaaccgcaac gacgttgatg atggcgtgca ggatatccag aatgatgtca
      181 atcaattagc tgacagcctc gaatctgtat tgaagtcctg gggcagcgac gccaaagggg
      241 aagctgaagc cgcacgcagt aaagctcagg cgttgctgaa agaaaccagg gcgcgaatgc
      301 atggtcgtac tcgcgtccag caagccgcgc gcgatgccgt tggctgcgcg gattcttttg
      361 ttcgtgaaag accctggtgt agcgtgggta cagcagctgc ggtaggtatt tttatcggcg
      421 cactgttaag catgcgcaaa tcgtagtgca aaaatgataa taaatacgcg tctttgaccc
      481 cgaagcctgt cttcggggtt tctttttgcc tggtgaatca caaaaatccc cctcccccgt
      541 cacgctcata tccagggtaa tttcgatcac tatttgctat atattgtgta gttgaatctt
      601 ttttcaacta catctagtat ctctgtatca acagagagac aacccgacgc gtatcatcgc
      661 gccgtatctt cattttaaac ggaaatacga atcatgcgca taactattta cactcgtaac
      721 gattgcgttc agtgccacgc caccaaacgg gcgatggaaa accgggggtt tgattttgaa
      781 atgattaatg tcgatcgcgt tcctgaagca gcggaagcgt tgcgtgctca gggctttcgt
      841 cagttgccgg tagtgattgc tggcgatctt agctggtctg gtttccgtcc ggacatgatt
      901 aaccgtctgc atccagcgcc acacgcggcc agtgcatgag ccagctcgtc tacttctcca
      961 gcagctccga aaacacgcag cgttttatcg aacgtttagg tctgcccgcg gtgcgcatcc
     1021 cgctcaatga gcgggaacgg attcaggtag acgagcctta catcctgatc gtgccctctt
     1081 acggcggcgg cggtacggct ggcgcggtgc cacgacaggt aattcgcttt ttaaacgacg
     1141 agcacaaccg ggcgttgctt cgcggcgtta ttgcttctgg taatcgcaac tttggtgagg
     1201 cgtatggccg cgccggagat gtgattgccc ggaaatgcgg cgtgccgtgg ctgtaccgtt
     1261 ttgaactcat gggtacgcaa agcgatatcg aaaacgttcg taaaggagta accgaatttt
     1321 ggcaacgaca accgcagaat gcctgacgca ggaaacgatg gattaccacg cgctgaatgc
     1381 gatgcttaac ctctacgata gcgcaggtcg cattcagttc gataaagacc gccaggccgt
     1441 tgacgccttt atggcgacgc atgtgcgtcc gaacagtgtg accttcagta gccagcagca
     1501 gcgcctgaac tggctggtca acgaaggtta ctatgatgaa agcgttctta atcgctactc
     1561 tcgcgacttt gtcattacgc tgtttgccta cgcacacacc agcggttttc gtttccagac
     1621 attcctcggg gcatggaagt tttacaccag ctatacgttg aagacattcg acggtaaacg
     1681 ttatctggaa gattttgccg atcgagtcac gatggtggcg ctgacgctgg cacaaggcga
     1741 tgcgacgctg gcgttgcaac tgaccgatga aatgctgtca ggacgctttc agccagccac
     1801 gccgacattc ctcaactgcg gtaagcagca gcgcggcgaa ctggtttcct gttttctgct
     1861 gcgtattgaa gacaatatgg agtcgattgg ccgggcggta aattccgcac tgcaactgtc
     1921 gaaacgcggc ggcggcgtgg catttttgct gtcgaatctg cgagaagcgg gcgcgccaat
     1981 taaacgtatt gaaaatcaat cttctggcgt aattccggtg atgaaaatgc tggaagacgc
     2041 attttcctat gccaaccaac tcggcgcgcg tcagggggca ggtgcagtct atttacatgc
     2101 tcatcatccc gatattctgc gttttctcga tacaaaacgg gaaaatgccg acgaaaaaat
     2161 ccgcattaaa acactgtcgc tgggcgtggt gatcccggat atcactttcc atctggcaaa
     2221 agagaatgtg cagatggcgc tgttttcgcc ctatgacgtt gagcgagttt atggcaagcc
     2281 gtttgccgat atcgccatca gcgaacacta tgacgaactg gttgccgatg aacgcatccg
     2341 caaaaaatac ctcaacgccc gtgatttctt ccagcggctg gcagaaatcc agtttgagtc
     2401 cggctatccc tacatcatgt atgaagacac ggtaaaccgt gctaaccctg tcgccgggcg
     2461 cataaatatg agtaatctct gctcagagat tttgcaggtc aactgtgcct cagagtatga
     2521 cgagaatctc gactatgccc gcacaggcca tgatatctcc tgcaatttag gttcgttgaa
     2581 tattgcgcac accatggatt cccccgattt tgcccgcacg gtagagactg ccgtgcgcgg
     2641 tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg ccgtccatcg aagccggaaa
     2701 tgccgcctca cacgccatcg gactggggca gatgaattta cacggctatc tggcgcgaga
     2761 aggcatcgct tatggttcgc cggaagcact ggatttcacc aatctctatt tctatgccat
     2821 cacctggcac gcactgcgta cctcgatgtt gctggcgcgc gaacgcggtg aaaccttcgc
     2881 cgggttcaaa cagtcacgtt atgccagtgg tgaatatttt agccaatatc tgcaaggtaa
     2941 ctggcagccg aaaacggcga aagttggcga actgtttgcc cgcagcggta ttacgttacc
     3001 tacccgtgag atgtgggcgc aactgcgcga cgacgtgatg cgctacggca tttataacca
     3061 gaatcttcag gcggtgccgc caaccggttc tatctcttat atcaaccatg ctacgtcgag
     3121 tattcatccg attgtggcga aagtagagat acgcaaagag ggcaaaacag gacgcgttta
     3181 ctaccctgcc ccgtttatga ctaacgagaa tctggcgctg tatcaggatg cttacgaaat
     3241 cggcgcagaa aagatcatcg acacctacgc cgaagcgact cgccatgtcg atcaggggct
     3301 gtcgctgacg ctgtttttcc ccgataccgc caccactcgc gatatcaaca aagcgcagat
     3361 ttacgcctgg cgcaagggta tcaaaacgct ctattacatt cgcctgcgtc agatggcgct
     3421 ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt taaggaatat ctatgaaact
     3481 ctcacgtatc agcgccatca actggaacaa gatatctgac gataaagatc tggaggtgtg
     3541 gaatcgcctg accagcaatt tctggctacc agaaaaggtg ccgctgtcga acgatattcc
     3601 tgcctggcag acattaactg tcgtagaaca acaactgacg atgcgcgttt ttactggcct
     3661 gacgctgctc gacacgctgc aaaatgttat cggcgcgcct tcgctgatgc cctatgcact
     3721 cacgcctcat gaagaagcgg tattatcgaa tatcagcttt atggaagcgg ttcatgcccg
     3781 ctcttacagt tcgattttct cgacgctgtg tcagaccaaa gatgtcgatg ccgcctacgc
     3841 ctggagtgaa gaaaacgcac cgttgcagcg aaaagctcag attattcagc aacattatcg
     3901 cggtgatgat ccgctgaaaa agaaaatcgc cagtgtgttt cttgaatctt ttttgttcta
     3961 ttccggtttc tggctgccga tgtatttttc cagccgcgga aagctgacca ataccgcaga
     4021 tctgatccgt ctgattatcc gcgatgaagc agtccacggt tactacatag gctataaata
     4081 tcagaaaaac atggaaaaga tatctctggg acaacgtgaa gagttgaaga gtttcgcctt
     4141 cgatttgttg ctggaactct acgacaacga gttgcaatac agcgatgagc tgtacgccga
     4201 aaccccgtgg gctgacgatg tgaaagcgtt tctctgttac aacgccaata aggctttgat
     4261 gaatctgggc tacgaaccgt tatttcccgc agaaatggcg gaagtgaatc cggcaatcct
     4321 cgccgcgctt tcgccgaatg ccgatgaaaa tcacgatttc ttttccggtt cacgtatagg
     4381 aaattgaaaa actaagcgat acccctgtcc gcgcctta
//
LOCUS       DAJVOY010000239         4380 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVOY010000239 DAJVOY010000000
VERSION     DAJVOY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.242"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(24..1238)
                     /gene="ydcO"
                     /locus_tag="OQL90_003861"
     CDS             complement(24..1238)
                     /gene="ydcO"
                     /locus_tag="OQL90_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCS1370468.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVARATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAEILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTVASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITEMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNQY"
     gene            1291..1827
                     /gene="sutR"
                     /locus_tag="OQL90_003862"
     CDS             1291..1827
                     /gene="sutR"
                     /locus_tag="OQL90_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCS1370469.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1900..3861
                     /gene="rlhA"
                     /locus_tag="OQL90_003863"
     CDS             1900..3861
                     /gene="rlhA"
                     /locus_tag="OQL90_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCS1370470.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMNYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVAEPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3953..4183)
                     /locus_tag="OQL90_003864"
     CDS             complement(3953..4183)
                     /locus_tag="OQL90_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCS1370471.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1136 a         1127 c         1089 g         1028 t
ORIGIN      
        1 cgtctttcag ggcgtgtagg atgtcaatat tggtttctgt cagcgattaa attcaacacc
       61 acgtaacaaa cgcctccggc aattaatccc cagaacgcag aaccaatccc gaccagcgtc
      121 aatccacttg ccgttaccag aaatgccacc accgccgcgt cgcgctcacg ctcattatgc
      181 agcgcctgat acaaactgcc gccaatggta ctcaacagcg ccagacctgc cagcatctgg
      241 atccaactta cgggcagggc agccatcatc tcggtaatgg cactaccaaa cagacctgcg
      301 agcaaataga aaatgcctgc aacggcagct gccagccaac gttgatcttt atccggatgc
      361 gcttccgggc tttggcaaat agccgcggtg attgccgcaa taccgacgga ataaacgccg
      421 aaaggggaaa aaaccagtgc cagcaatcca gtaaatacaa ttaatggcga aacaggagcc
      481 gaatatccag ctgctttcat tgctgcgata cccggtgcgt tttgcgatgc caccgtcacc
      541 agaaaaaggg ggagtgcaac gctcaggctg tgagcaaacg aaaaatcagg ggtaatataa
      601 gtggggagaa cgggtttaaa gacaacatca gttgtgacaa cgtcaccttg cgcgatgacg
      661 atcacgatcc caataatcat cgcggcaatt accgcatagc gcggcgcaac ggccttggtt
      721 gccagccata ccagcaacat acttccacac aacgtaaatt gaccgtccag actggcaaac
      781 gcctgtaaac caaagcgtaa taaaatctcg gcaagcattg ccgccgcaag cgagtgcgga
      841 ataatgcgca tcagacgagc aaagagtccc gttatgccgc agaggactat taacgcgttg
      901 gtgacaataa aaacgccgat ggcttcgtta agtgttagtc cctgcaatcc ggtgaccaac
      961 aaagccgcgc caggcgttga ccatgcggtg agaacaggta cgcgatacca taatgtcaga
     1021 gtcagcgtac tgacgcccat tgccagcccc agcgccgtca tccagccaga gatttgtgca
     1081 gtggtggctc tggcgacaat cgctgcttgc cagattattg ccgctgaact ggcgtagcca
     1141 attaataccg ccagaaaccc cgccagtagc gtgggtggag gaatagaaaa cagacgcata
     1201 gtcacctcgt acgttatagc gtacaggcac cgtagcattt gtccgttata acgcacaagt
     1261 gataaacttc cgttttgccg gaggagtcgc atggaaaatc tcgctcgctt tttatccacc
     1321 acacttaaac aactacgcca gcagcgcggc tggagtcttt cgcgactggc agaagcgacg
     1381 ggcgtttcta aagcaatgtt agggcagatt gagcgtaatg agtccagtcc gacggtcgcg
     1441 acattatgga aaattgccac cgggttgaac gtaccatttt caacatttat ttctccgccg
     1501 cagtccgcta cgccttcagt ttatgatcca caacagcagg cgatggtgat cacttcgctg
     1561 tttccttatg atccacaact ctgctttgaa cacttctcga ttcagatggc atctggtgca
     1621 ataagcgaat cgacgcccca tgagaagggg gtaatcgaac atgtcgtcgt cattgatgga
     1681 caacttgatc tgtgcgttga tggggaatgg cagaccctta attgtgggga aggcgttcga
     1741 tttgctgcag acgtcacgca tatctatcgt aatggtgggg agcaaaccgt acattttcat
     1801 tccctcatcc attacccgcg cagttaagcg ggaaaactat ttcgcaacgt cgcgcttgtg
     1861 gctaaaatag ccgccatttt tcagctactg gataagaatg tgaccgtatc ttctcatcga
     1921 cttgaactgt taagcccggc acgcgatgcc gccattgccc gcgaagctat tttgcacggt
     1981 gccgatgctg tttatatcgg cggccctggt tttggtgccc gtcataatgc cagtaatagc
     2041 ctgaaagata ttgccgagct ggtgccgttt gcccatcgtt atggtgcaaa aattttcgtc
     2101 acgcttaaca ccattttgca tgatgatgag ctggaacccg cgcaacggct gattactgac
     2161 ctctaccaga ccggtgtcga tgcgctgatt gttcaggata tggggattct ggaacttgat
     2221 attccgccga ttgaactgca cgccagtacg cagtgcgaca ttcgtacagt tgaaaaagcg
     2281 aagttcctct ctgatgttgg cttcacgcag attgtgctgg cgcgagagct gaatcttgat
     2341 cagatccgcg cgattcacca ggctacggac gcgaccattg aattctttat tcatggggca
     2401 ctgtgcgtgg cctattcggg tcagtgctac atttctcatg cgcaaacagg gcgtagcgcc
     2461 aaccgtggcg attgctcgca ggcgtgccgt ttgccataca cattgaaaga cgatcagggg
     2521 cgggtggttt cctatgaaaa acatctgctg tcgatgaaag ataacgatca gactgccaac
     2581 ctcggcgcgc tgattgatgc tggtgtacgc tccttcaaga ttgaagggcg ttacaaagat
     2641 atgaactacg tgaagaatat caccgcccat tatcgccaga tgcttgatgc cattattgaa
     2701 gaacgtggcg atctggcgcg cgcttcatca ggtcgtactg aacatttctt tgttccatcg
     2761 acggaaaaga ctttccaccg tggtagcaca gattattttg tgaatgcccg taaaggcgat
     2821 attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg tgggtgaagt attgaaagtg
     2881 gcgaaagatc atctcgatgt tgccgtcacc gagccactgg caaatggtga tggcctgaac
     2941 gtgatgatta aacgtgaagt catcggtttt cgtgccaata cggtcgagaa aaccggagaa
     3001 aatcagtacc gcgtctggcc caatgaaatg ccagcagatt tgcacaaaat tcgtccacat
     3061 cacccactaa accgtaatct tgatcataac tggcagcagg cactgacaaa aacctccagc
     3121 gaacgtcggg tggcggtaga cattgaactg ggcggctggc aggaacaact gattctgacc
     3181 ctcaccagtg aagagggtgt cagcatcacg catacgctgg acgggcagtt cgacgaagcc
     3241 aataacgccg aaaaagcaat gaacaatctg aaggatggtc tggcaaaact ggggcaaacc
     3301 ctctattacg cccgcgatgt gcaaattaat ttgccggggg cgctgtttgt accaaacagt
     3361 ctgttaaacc agttccgccg tgaagctgct gacatgctgg atgctgcgcg tcttgccagt
     3421 taccagcgcg gcagccgtaa accggttgct gagcctgcgc cggtttatcc gcaaacgcat
     3481 ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg aattttatca tcgctatggt
     3541 gtgcagctga ttgacgcggc gtatgaggca catgaagaga agggcgaagt cccggtgatg
     3601 atcaccaagc attgtctgcg ctttgccttt aatctgtgcc cgaaacaggc gaaaggcaat
     3661 atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg gcgatgaagt attaacgcta
     3721 aagtttgatt gccgcccatg cgagatgcac gtcattggca aaatcaaaaa tcacatactg
     3781 aaaatgccgt taccgggaag cgtagtggca tccgtaagtc cggatgagct gctgaaaaca
     3841 ttgcctaagc gaaaagggta aaacgccagt tttctggtta ctcacaactc attgaatctg
     3901 catgatattg cctgccgggt aaggcgtcac gccgcatccg gcatcaaacg actcaacgtt
     3961 gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg ctcgcgcaaa ccttccgccg
     4021 actcttccgc cacagcacgc aattcatcgc tgtccgcttc atgacgcagc tgatgatcca
     4081 cattctttac ccacacaaat tcatgtcctt tgtgccctgc catgagttgt cctgagaaca
     4141 gcgcacacgt taataagaca accgataacg ccttcgtaaa catcctgcca cctttttctt
     4201 accttttgcc gctatgatgc cgatcgtttc ttgaggttat tattcagttt tgcaaattag
     4261 cgcaaagaaa ttctggaatc ttccttcctg attttgcatt gcattctgtc gttgcggcga
     4321 tttagtgcta ttttcgagca aattacacac agaggtaaac actaatgttt actttgttga
//
LOCUS       DAJVOY010000240         4370 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVOY010000240 DAJVOY010000000
VERSION     DAJVOY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.243"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..339
                     /locus_tag="OQL90_003865"
     CDS             <1..339
                     /locus_tag="OQL90_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370472.1"
                     /translation="EGHLSSFFVQTDKASINVAYHHTVNQQKELGTFGAFDHDWHTLA
                     FKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDTVE
                     VKANKAGAAA"
     gene            479..661
                     /locus_tag="OQL90_003866"
     CDS             479..661
                     /locus_tag="OQL90_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCS1370473.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            585..932
                     /locus_tag="OQL90_003867"
     CDS             585..932
                     /locus_tag="OQL90_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCS1370474.1"
                     /translation="MIKGPLRKSAPVSQPEANPKNPLLLNNPFQRENPMSEITSLVTA
                     EAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDAILDELLGVAAVPEPEGIAGDGSAS
                     DGGEPTPDSDMMM"
     gene            1009..1224
                     /locus_tag="OQL90_003868"
     CDS             1009..1224
                     /locus_tag="OQL90_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1370475.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1229..1573
                     /locus_tag="OQL90_003869"
     CDS             1229..1573
                     /locus_tag="OQL90_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCS1370476.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1624..2157
                     /locus_tag="OQL90_003870"
     CDS             1624..2157
                     /locus_tag="OQL90_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1370477.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2313..2495
                     /locus_tag="OQL90_003871"
     CDS             2313..2495
                     /locus_tag="OQL90_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370478.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            2822..2974
                     /locus_tag="OQL90_003872"
     CDS             2822..2974
                     /locus_tag="OQL90_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCS1370479.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPAPDWQTPLNGII
                     SPSESG"
     gene            3060..3284
                     /locus_tag="OQL90_003873"
     CDS             3060..3284
                     /locus_tag="OQL90_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370480.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            3629..3955
                     /locus_tag="OQL90_003874"
     CDS             3629..3955
                     /locus_tag="OQL90_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCS1370481.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(4087..4287)
                     /locus_tag="OQL90_003875"
     CDS             complement(4087..4287)
                     /locus_tag="OQL90_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCS1370482.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
BASE COUNT         1119 a          935 c         1197 g         1119 t
ORIGIN      
        1 gaaggccatc tgagttcttt ctttgtccag acagataaag ccagtattaa tgttgcatac
       61 catcatacag ttaatcaaca aaaagaactt ggcacgtttg gcgcattcga ccatgactgg
      121 catacgcttg catttaaatt taagggcagt aacagcatta atgttactcc ggtgcttgat
      181 ggtgtggatg gacaggcgtt tgacctggtg aaatgggcca atactgctaa tggactcaac
      241 aggtttgtca ttacggatat tacaggtagt gcagaaacct accctgtact tattgatacg
      301 gtggaagtta aagcaaacaa agctggagca gccgcataat tgctaaaaaa agccgccagc
      361 ggcaggaatg gaagctggcg gaggtaatcc caatggagaa tgtaaagaaa agatgctttc
      421 gtacattggt tttttaaatg aaaacaattc tcattgtcaa ccataacggt aagaaattat
      481 gacatttatt catcaggtga tgctgtactt ctgtacggcg gtctgtgtgc tgtatcttct
      541 ttcgggtggg tacagggctg tgcgcgattt ctggtgcagg cagattgata aaagggccgc
      601 tgagaaaatc agcgccagtc agtcagccgg aagcaaaccc gaagaacccg ttactcctta
      661 acaacccctt tcagcgagaa aatcccatgt cagaaatcac atccctggtc actgcagagg
      721 cagtgaagga agtcctgcgc tctgaagaag tccggagcgc actgaaacag aaacttcgcc
      781 agaatcttga gtcgcgtctt gatgcagaag tggatgccat tctggatgag ctgctgggcg
      841 tagcagcggt tcctgagccg gaaggtatcg cgggtgacgg gagtgcttca gatggcggtg
      901 aacctacacc tgacagcgac atgatgatgt aagcatgcgc aagggaccat cggtgtgtgc
      961 cggtggtctt tatattgttg tgagcttccg gattgcggga gacggggtat gtaccagatg
     1021 gaaaaaatca caacaggtgt gtcatacacc acgtcagcgg tgggaacggg ctactggttc
     1081 ctgcagttgc tggacaggtt ttccccgtct cagtgggcgg caataggcgt gctggggagt
     1141 ctgttgtttg ggctgctgac atatctgact aacctgtatt tcaaaatcag agaggaccgt
     1201 cgtaaggcgg cgcggggaga gtaaagcgat gaagaaaaaa tacgaactgg ttgttaaagg
     1261 gataaataat tacccgaata agattactgt tactgtggca ctggaaattg gtgggtatcc
     1321 gtcactgttg ttgccagatg tggcgattag tcttgaccgt actgaagatg ccacgctgga
     1381 gttttacgaa gctgaggcga aaaagcaggc gaagcagttt ttcatggatg ttgctgccgg
     1441 gttatgtgaa ggggatggtc cgttgccgga aaagcgcccc gtaattttag aggcgcagga
     1501 tgtgttgata acctacagag gaaaactacc gggaataatt actggttctc tgaagactcc
     1561 accgctggcc tgaagactta acatatccag ggatttgaaa tcgataaacc ctgataaata
     1621 tccatgaaca ccaaaatcaa atacggcctg tcggctgccg ttctggcgct gattgccgca
     1681 ggtgcgcctg cgcctgaaat cctcgaccag tttctggatg aaaaggaagg taaccacacc
     1741 acggcatacc gtgatggcgc gggtatctgg accatctgcc gcggtgccat cctggtggat
     1801 agtaaacctg tcgtcccggg catgaagttg tcgaaggaaa aatgcgacca ggtcaacgcc
     1861 attgagcgtg ataaggcgct ggcatgggtg gagcgcaata ttaaagtgcc gctgaccgaa
     1921 ccccagaaag cgggtatcgc gtcattttgt ccctataaca ttggccccgg taagtgtttc
     1981 ccgtcgacgt tttacagacg gattaatgct ggtgaccgca ggggagcatg cgaagcgatt
     2041 cgctggtgga ttaaggacgg tggcagagac tgccgtatcc gctcaaataa ttgctacggt
     2101 caggtatccc ggcgagacca ggaaagtgcg ctggcgtgct gggacatcga cagataagca
     2161 gaatattttg ctgaaaataa ggcatgagca cgcggacgga taacacgaaa tcctgcgaac
     2221 tggcgaaacg taagtgaata aaagtaaaaa ccccgtttgt tggcaccagg cggggttttg
     2281 tgtttctgac cttgagtaag gcaagggaga acatggcgaa gtataaacga attctgttga
     2341 ggttgaccat gaaaaacggc cttgaactga aagcgcctgt aactgatgac atcagcagag
     2401 cactggcttt tgccattaag tgggtggcgg tcggtgttgc tgtgtccccg atgctgtatg
     2461 ggctggcaaa actggtcatt gcgttgaaat cgtgaaggga ggattaagca tgtcagacaa
     2521 actcataacg ctggcgaaga tcctctgtgt aattgtcggc atttcatttt cactaatgct
     2581 ggttgctctt tttctttcca tggcctggat gatgttgtct tcgtcggggc tgctggggtg
     2641 aacataaacc gaatgctttc cgcgtttacc gttattctgc tggtggtctg tggtgcgctg
     2701 tggctggcaa cagaccatta ccgtgataac gccatcacct acaaagcgca gcgcgataaa
     2761 aaagccagag agctggagct ggcaaacgca accattactg acatgcagca gcgccagcgt
     2821 gatgttgccg ctggtcgtaa gcgcctgcgg atcaacgcca cctgctccgg taccgtgcgt
     2881 gaagccaccg gcacctccgg aatggataat gcaaccggcc cccgactggc agacaccgct
     2941 gaacgggatt atttcaccct cagagagcgg ttgatgacaa tgcagaagca actggaaggg
     3001 gcgcaggaat atatccgcac tcagtgtatt aactagtatt tttgttatcc ggagaatgca
     3061 tgaagaaatt actggtaacc gtaaagcctt ttcagggaac aattccgttc cgtattttgc
     3121 agcgtggtcg tgttcttgtt gaaggttcgt tcagtggtaa atgtacgcaa ttacactccc
     3181 ggacctttca ggtgaatgcc acgaatgaag agctaaccgt tgagtgtacg atgaatgccg
     3241 ctaaatgccg catggtatcc gctgcattac agccagtgtg ttgagcgacc ttattaacca
     3301 tgcgcggtat tgtcgccgta tccccgcatt aacagagacc gcagcccgac cgggagactc
     3361 ctctgcgcga gtgtgcgggg ataatcaaaa acgatacaca ccggggttta ccgcgttaac
     3421 ggagcgcggc gttgtcccct catggtcgct ggtccggtgc gatggtggaa gaaaccggac
     3481 gatgtgttac ctcgcaagct ctgttatgtc atgtgtctga tttgtgattt aagtcggata
     3541 attgtcgttg ccattaagca gaggattgat gaccgacagg gtggcattgt tagaataaga
     3601 cttattctta tctgtgccgg gaatgaaaat gaaaagaaac cttccgttaa ttattttgtt
     3661 gtcttctctg gttatgggct gtacgcaaca taaaacagat atgccccgac agttggttaa
     3721 ggcattacca caatatccgg cctatgcagc ggcaaattat ataaagggac gggttgatgt
     3781 gaagtttgat attggtgctg atggtactgt cacccgaatt gagtttatcc gttcagagcc
     3841 gcaccatttg tttgatgagc aggttgtaaa ggcgatggca aaatggcgat ttgagaagga
     3901 caggccgtgt aaaggcgtga agaaaacgtt tatctttagt ccttctgcac cctgattatt
     3961 tcatcagaaa ttaattatca ctctgttgtt attctgtaca tcccggcagg gtaagtcttg
     4021 ttccgtcgga tatgaagatg aaatattgtt ggaggacagt gggtacctgc tcctgtaacc
     4081 gaacgttcat ttctcgttat ttttcatgct ggccgggcgc agatgcgttg catctgttgc
     4141 cagccttctc ctgcaggctt caataaccca cgctgaaaag tttcctgaac ctttcagatc
     4201 aagagcgatg ttaatttgtt caatcatctg gtttggaaat cggatgttgc gggttgttgt
     4261 tctgcgggtt ctgttctttg atgacataat gtttccccat attcagtgtt gctgatttgt
     4321 attatctgaa gttgctttta cgttaatttg atgcagatca atcaatacga
//
LOCUS       DAJVOY010000241         4288 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVOY010000241 DAJVOY010000000
VERSION     DAJVOY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.244"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            40..2202
                     /gene="ybiO"
                     /locus_tag="OQL90_003876"
     CDS             40..2202
                     /gene="ybiO"
                     /locus_tag="OQL90_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCS1370483.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2262..3188)
                     /gene="rlmF"
                     /locus_tag="OQL90_003877"
     CDS             complement(2262..3188)
                     /gene="rlmF"
                     /locus_tag="OQL90_003877"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1370484.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3464..3723
                     /locus_tag="OQL90_003878"
                     /pseudo
     CDS             3464..3723
                     /locus_tag="OQL90_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            3988..>4288
                     /locus_tag="OQL90_003879"
     CDS             3988..>4288
                     /locus_tag="OQL90_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1370485.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          996 a         1129 c         1076 g         1087 t
ORIGIN      
        1 atacttatct ttttgctctg ttttctcact ggaggagtca tgcggtggat cctgttcatc
       61 ctcttctgcc tgctgggcgc acctgcccac gcggtatcca tacccggcgt tacaaccaca
      121 acgacaacgg actcaacgac tgaaccggcc ccggaaccgg atatcgaaca aaaaaaagcg
      181 gcctatggcg cactggcgga tgtgctggat aacgacacct cgcgtaaaga attgatcgac
      241 cagttgcgta ctgtcgccgc gacgccccct gcggaaccgg tcccgaagat cgtgccgccg
      301 acgctggttg aagagcaaac cgtgctgcaa aaggtcaccg aagtcagccg ccattatggt
      361 gaagcccttt ccgcccgctt cgggcaactt tatcgcaata ttaccggctc cccgcataag
      421 ccgtttaatc cacaaacatt cagcaatgcg ctgacccatt tttcaatgtt agcggtatta
      481 gtgtttggtt tttactggct gattcgcctg tgcgcactgc cgctgtatcg caaaatgggc
      541 ctgtgggcgc ggcaaaaaaa tcgtgagcgc agtaactggt tgcagcttcc ggcgatgatt
      601 atcggggcgt ttattatcga cctactgtta ctggcactga cattatttgt cggccaggta
      661 ttaagcgaca acctgaatgc aggcagtcgc accatcgctt tccaacaaag tttgtttctc
      721 aacgcctttg ccctgattga atttttcaaa gctgtactac gcctgatttt ttgcccaaac
      781 gtggctgaac tgcgcccgtt cacgattcat gacgagaccg cccgttactg gagtcgtcgc
      841 ctgagctggt taagcagttt gataggttat ggcctgattg tggccgtgcc gattatctct
      901 aatcaggtga atgtacagat aggtgcgctg gcgaacgtca tcattatgct gtgcatgacc
      961 gtctgggcgt tgtacctgat ctttcgcaat aaaaaagaga ttacccaaca tttgctcaac
     1021 tttgcggagc attcgctggc ctttttcagc ctgtttatcc gcgcctttgc gctggtgtgg
     1081 cactggctgg caagcgccta ttttatcgtg ctgtttttct tttcgttgtt cgatccgggc
     1141 aacagcctga aatttatgat gggtgcaacg gtgcgcagcc tggcgattat tggtatcgca
     1201 gcgtttgttt ccggtatgtt ttcccgctgg ctggcgaaaa ccatcaccct ctcgccacat
     1261 actcagcgta actatccgga gttgcaaaaa cggctgaatg gctggctttc ggcggcgctg
     1321 aaaacggcgc gtattctgac cgtctgcgtg gcggtaatgt tgctgttgag cgcatgggga
     1381 ttgttcgatt tctggaactg gctgcaaaac ggcgcggggc agaaaaccgt agatatcctg
     1441 atccgtatcg cacttattct ttttttctcg gcggttggct ggacggtgct cgccagtttg
     1501 atcgaaaacc ggctggcttc ggatattcat ggccgcccgc tacccagcgc ccgtacgcgt
     1561 accctgctga cgctgtttcg taacgcgctg gcggtgatta tcagtaccat caccatcatg
     1621 attgtgttgt cggaaatcgg cgtcaatatc gcgccattgc tggcaggtgc cggggcatta
     1681 ggtctggcta tctcgtttgg ttcgcaaacg ctggtgaaag atatcattac cggggtgttt
     1741 attcagtttg aaaacggcat gaacactgga gatttggtga ctatcgggcc gttgaccggc
     1801 acagtggaac ggatgtcgat tcgctccgtg ggcgtgcgcc aggataccgg ggcgtatcac
     1861 atcattccgt ggtcttcgat aaccaccttt gctaacttcg tccgcggtat tggttcggta
     1921 gtggcaaact acgatgttga tcgccatgaa gatgctgata aagccaatca ggcactgaaa
     1981 gatgcggtag cggaattgat ggaaaacgaa gaaattcgcg ggctgattat tggtgaaccg
     2041 aattttgccg ggattgtcgg cttaagcaat accgcgttta cactgcgtgt ttcgttcacc
     2101 acgctgccac tcaaacagtg gacggtacgc tttgccctcg acagccaggt gaaaaaacat
     2161 ttcgacctgg tgggcgttcg cgcgccagtg cagacttatt aggtgctgcc tactccgggc
     2221 gcgaccccgg ctgaaccgtt accgccgggg gagccaacgc tttaacgctg gcgattgaca
     2281 aaacggcggc gctgctcgtc gttcataaag gtccaggcaa taaagcgact ctgcttttgc
     2341 ccctgggcca tctctttttt taccaccttc accgcgccca cgtcggtcag agcacgatac
     2401 aacggcggta agttttcacc acgagatacc agcgaggtaa accacatcac ctgcttcgcg
     2461 aagcctttgc tctcttcaat catctttttg ataaaggcga cttcaccacc ttcacaccac
     2521 aactcctgtt gctgaccacc aaagttcagt gcatcgtctt tgttcagccc caggttacgg
     2581 cgtttacgct cactacctgc ccgcgctgca gcggcggaat cgtggaatgg cgggttacac
     2641 aaggtcgcgt cgtattgctc gtttttatgg atgatgccgt taaaaattgc cccactctct
     2701 ttttgccgac gcagacgaat ggcgcggtta agccccggat tagcactgat aatcgcctgc
     2761 gcactgctta acgcctggct gctggtttca ctaccggtaa aacgccagcc atattcatgt
     2821 acgccaatca gcggataaat acagttcgca ccaacgccga tatccagaat gctggcattc
     2881 gctggaattg ttccgcttgc ttcagccagt aaatcggcaa ggtgatgaat ataatccgcc
     2941 cggccgggta ctggtgggca gagaaaaccg tcggggatat cccagttcgc tacggcgtaa
     3001 aaatgggcca gcaacgcctt attgagcgcc ttcaccgcca gcggattggc aaagtctacg
     3061 ctttgctccc cggcgggtgt aagcgtgagg aattgcctga gttcaggatt gacctgacaa
     3121 agcgtggcga gatcgtagcg gctgtgatga cggttgcgcg gatgcaaccc cggtttctgg
     3181 gcggacatag cattctcctt taagcagcgg cgtaagatac ccgcataacg cgacagggta
     3241 aatatctggc tgcgtttatc attccccaaa accttaacat ggctatggtt tatggcaatt
     3301 catcataaaa atcagcatta tgcatgtcat tgatttgtct tattttgtaa aaatatatcg
     3361 ttcaaaaaat aagcacatct ctgtcaattt tttgcgtcat ttgcatcgcg caggcgacct
     3421 acacttatca ggcactaccc gttgtattta tgaggatgtc attatgaaaa agtgcctcac
     3481 actactgatt gccaccgtcc tgagcagaat ctctctcacg gcttatgccg cgcaaccgat
     3541 gagtaacctg gacagcggtc agctacggcc cgcggtaccg tttcggcaac tggcgcatca
     3601 aacctaagcg atctggagga taaactggca gaaaaagcgc gcgaacaagg cgcgaaaggt
     3661 tatgtcatta attccgcagg cggaaatgac cagatgttcg gtactgcaac catctacaaa
     3721 taaccccacc aacgtaactg cattcgcctc tggatgcagt tttcgcctgt tttgtaatca
     3781 aaaaacatca aatatgaact caatgtaaat aaatgtattt ctttttcgcg caatgggtga
     3841 tagaaaatcg cgccaagtga taatgcttat caaaattatt atcacttgca cgaacactat
     3901 cacgggatta acagtggcat cgcatccgca gagatgcttt ctcgtgacag tgaaaatttc
     3961 aacatataag aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc tgccagacaa
     4021 tttcattcgc tcacgttctt tgccggtctt tgtattggca tcacgcctgt ggctcaggca
     4081 ctcgccgccg aagggcaaac taacgcggat gacacgctgg ttgtcgaagc atcaacgcct
     4141 tcgctttatg cgccacaaca atctgccgat ccgaaattct cgcgtccggt agcggatact
     4201 acccgcacga tgacggtgat ttctgaacaa gtgattaaag atcagggcgc aaccaacctt
     4261 accgacgcgc tcaaaaacgt ccccggcg
//
LOCUS       DAJVOY010000242         4226 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVOY010000242 DAJVOY010000000
VERSION     DAJVOY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.245"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..882)
                     /locus_tag="OQL90_003880"
     CDS             complement(<1..882)
                     /locus_tag="OQL90_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCS1370486.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESI"
     gene            complement(1037..1663)
                     /gene="dsbA"
                     /locus_tag="OQL90_003881"
     CDS             complement(1037..1663)
                     /gene="dsbA"
                     /locus_tag="OQL90_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1370487.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1680..2666)
                     /gene="srkA"
                     /locus_tag="OQL90_003882"
     CDS             complement(1680..2666)
                     /gene="srkA"
                     /locus_tag="OQL90_003882"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1370488.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2743..3012)
                     /locus_tag="OQL90_003883"
     CDS             complement(2743..3012)
                     /locus_tag="OQL90_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1370489.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            3082..3666
                     /gene="mobA"
                     /locus_tag="OQL90_003884"
     CDS             3082..3666
                     /gene="mobA"
                     /locus_tag="OQL90_003884"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1370490.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3648..4175
                     /gene="mobB"
                     /locus_tag="OQL90_003885"
     CDS             3648..4175
                     /gene="mobB"
                     /locus_tag="OQL90_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1370491.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1147 a         1000 c          942 g         1137 t
ORIGIN      
        1 aatagattca gcaatcaatg accactcacc actaccgagt tgctgattcc cttttgttaa
       61 cttatcaagt tcgtaggtca gattgacatt gatataatct ttgtctttaa cgcgatccag
      121 cctggttttt gcaacgaaag catcgatttg ggcaacgtct tccccctgat tgataatatt
      181 aacctgatta aatttcgact cgctttcacc aaccggaaat ttcttttctt ctaatcgtgt
      241 cagagaatca atggtaaaac tttttgccgt tactttgttc attggtgata attgcagaac
      301 aatatcttcg actttgcctt caatatccag gttagacaat gaactaccat caccattaaa
      361 agtaaactgg ccctcaccaa aagaaaactt tccatactct gcaggggcaa catctaatgt
      421 cgtagagaat gagccaccaa acgcgaatcg caatgtcgct tccacgggcg atttattttt
      481 cgcggcgata aatagcggtt gtgtcagttc attattcact aagttcactt ttgccgctgc
      541 cagtgctggg atgacattac catgcattaa cattgtgatg ggcaacggac cgtgctcaac
      601 atccacatca aatgcaactt tctggcctgg cttgatattg agatcgggtg caccgttatc
      661 aaatgaaatt tgcatttgaa aatgggaatt aaaaaagcct ttgtcaaagt ttttataact
      721 taacgtcata tcgtaggcat gttcaccttt ctttttagca tcgttaaaat ctttaataaa
      781 tttttcgaca ccaggctgaa tttgcgtacc tgtgtaccat atgccaccac cccagattac
      841 ggctaacgca acaataacac ctgtagctga cttacgtatc atataaatat tccctgataa
      901 aacagcaaaa ctttgaatat ccacttatgc tgaaataatg tccagcggca ggatgtatta
      961 tcagaatgta tttatattta ttaaataata aaaaaagccc gtgaatattc acgggcttta
     1021 tgtaatttac attgaattat tttttctcgg acagatattt cactgtatca gcatactgct
     1081 gaacaaaaac atccatattg ctggtatcca taccctgcgg attcagctga tatttaccgt
     1141 taacaaacat cgccggaaca ccgcgcaatt gcacgtcagc tgcagctttt tcctgctgag
     1201 ctaccagaga tttcaccacg aagctgttcc acgccgcgtc gtactcttca cctttaatac
     1261 ctgcgttgat aaatacatcg cggatatcag aagcagaacg aatggtctgg gttttctgta
     1321 cgccttcaaa cagcggaaca gtcactttgt cttccacgcc cagcgccatc gccacagccc
     1381 atgcctgagt cagatctttg cccaggtcac cacccatgaa gttgacgtgg tatttagtca
     1441 tcttcacgcc ttccggcagt tttttcttca cattatcaga aatatgcaga acttcttcaa
     1501 actgatagca gtgcggacag aagaaagaga aaaactccag cacttgcggc gcgccagcta
     1561 ccggtttttc cagggtagtg tactgtttac catcttcata ctgcgccgcc gatgcgctaa
     1621 acgctaaaac taaaccagcc agcgccagcc aaatcttttt catgatctac tctctccgat
     1681 taatacatag gtgttaattg taaagggggt tcttgtagaa cttttgcctg ttctataaaa
     1741 gtcgccgttt gtcgcagcca gtaatcttcc ccggttaacc acgggaaatt tttcgggaac
     1801 gcgggatcag cccaacgccg cattagccag gcaagataat aaaccaaacg catggcgcgt
     1861 aaaggttcaa tcagtccgat ttcagcggtg tcgaactcgc taaattcttc ataagcttca
     1921 ataatagttt ccagttgcat ccgctgctcg gctttatcgc cattgagcaa catccacaaa
     1981 tcctgaatag ctggaccatt acgtgcatca tccagatcaa caaacattgg accatcgcgc
     2041 cagagaatat tcccggcgtg gcagtctcca tgtaagcgca gaacggtgga atcttcccgc
     2101 cagtgcgctg taacggcggc gatcagctca tctgtcgctt tcaggaatgc cgctttcaac
     2161 ccggaaggta tcagtgtagc gtcctcaaac agcttgcgtg gctcaatgag atattcattc
     2221 agaccgatgg tcgggcgatg gataaaaagc tgtttgcgcc ccgtctggtg catacgccct
     2281 aaataacgcc caaccgcttc catctgatcg atattatcag cttcgaactg gcgaccaccg
     2341 acgcttggaa aaacagcgaa ataaaatccc tgatgattca ataaagtctg accgttaaag
     2401 gccacaggtg ctgcgaccgg aacttcatca tttaccaact gcaacgcaaa ttgatgttct
     2461 tcgaggattt gatcggctgt ccaacgttca gggcgataaa atttgacgac aatacgtcga
     2521 cgatcttcgt cctgaaattg atagacacgg ttttcatagc tgttaagcgg ggtaagaccg
     2581 gaatccaccc ggatcccttg ctcaaataga gcatccatga tggtatccgg gtgtagtgtc
     2641 tggaaagtaa aagcgctgtt gttcatccca tcatccggaa aacattacga atgatgaagg
     2701 atatcatttc acgatgattt tggtggcgcc gcttacaagc ttttactctt taattacgcc
     2761 gcgagcgcgt aacagcgccg ttttgaagtc ttcttcataa tctttctgca aacccgggat
     2821 caccgcatct ttcgcggaat cacgcatttt caggtgatag atcagaatgt catccgtcaa
     2881 atccgccagt tcgccgtcaa aacctgactc tttcgccagt ttctgcaaaa attgcaacag
     2941 gttaaggtct ggctcttttt gccaggctgg ctggaggaat tcaataactt cattcagacg
     3001 tttacatttc atagtgatgc tccttactct tgagacagac acgttagcag ggtcaatccc
     3061 acaataaaag aggcgatatc ggtgaatctg atgacgacga taacaggcgt tgtgctggca
     3121 ggcggtaaag ccagacgaat gggtggcgta gataaaggat tgcttgaatt aaacggcaaa
     3181 ccattatggc aacatgtcgc tgacgcgctt atgacgcagc tctctcacgt cgtggttaat
     3241 gccaatcgtc atcaggaaat ctatcaggca agcggtctga aagtgattga agattcactg
     3301 gcggattacc caggccctct ggcaggaatg ctttcagtaa tgcagcagga agctggtgag
     3361 tggtttttgt tttgcccgtg cgatacgcct tacattcccc ctgatttagc agcccggctt
     3421 aatcatcagc gcaaagatgc gcctgtcgtg tgggtccatg acggtgaacg cgatcacccg
     3481 actattgctc tggtaaaccg cgctattgag cctttattac tggaatatct gcaagcagga
     3541 gaacgccggg taatggcatt tatgcgtctg gctggcggtc atgcggttga tttcagcgat
     3601 cataaagatg catttgttaa cgtgaatacg ccagaggagc ttgcccgatg gcaggaaaaa
     3661 cgatgatacc gttactcgcc tttgccgcgt ggagtggtac cggaaaaact acgcttttga
     3721 aaaaactgat cccggcatta tgcgccagag ggatccgtcc agggctgatt aagcatacgc
     3781 accatgatat ggatgttgat aagccaggca aagatagcta tgagctgcgc aaggctggcg
     3841 cggcacaaac catcgttgcc agccagcaac gatgggcctt gatgacggaa acaccagacg
     3901 aagaagagct ggatctacag tttctcgcaa gtcgaatgga tacctcaaag ctggatttga
     3961 ttctggtcga agggtttaag catgaagaga tcgcaaagat tgtgctgttt cgcgatggag
     4021 ccagacatcg acctgaagaa ttagtgatag acaggcatgt tattgctgta gccagtgatg
     4081 tgccgcttaa tcttgatgtc gcattactgg atattaatga tgttgagggg ctggctgatt
     4141 ttgtggtgga gtggatgcaa aagcagaacg gataagcaag agtaggctgg atcagacgca
     4201 gccagtgtcg catccgacct ttttct
//
LOCUS       DAJVOY010000243         4220 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVOY010000243 DAJVOY010000000
VERSION     DAJVOY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.246"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..815)
                     /locus_tag="OQL90_003886"
     CDS             complement(<1..815)
                     /locus_tag="OQL90_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCS1370492.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLVDTYTSLNRQYVELQSEYKHLRRYFGVTAQVPYTDVWT"
     gene            complement(923..1163)
                     /locus_tag="OQL90_003887"
                     /pseudo
     CDS             complement(923..1163)
                     /locus_tag="OQL90_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1390..2211)
                     /locus_tag="OQL90_003888"
     CDS             complement(1390..2211)
                     /locus_tag="OQL90_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1370493.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            complement(2208..2588)
                     /locus_tag="OQL90_003889"
     CDS             complement(2208..2588)
                     /locus_tag="OQL90_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1370494.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(2589..3572)
                     /locus_tag="OQL90_003890"
     CDS             complement(2589..3572)
                     /locus_tag="OQL90_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1370495.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     DERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQNPPQEKTVVSI
                     AVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGYGLGGMGTKA
                     DDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(3646..3918)
                     /locus_tag="OQL90_003891"
     CDS             complement(3646..3918)
                     /locus_tag="OQL90_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370496.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(4086..>4220)
                     /gene="hokD"
                     /locus_tag="OQL90_003892"
     CDS             complement(4086..>4220)
                     /gene="hokD"
                     /locus_tag="OQL90_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1370497.1"
                     /translation="LIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE
                     "
BASE COUNT          970 a         1192 c         1000 g         1058 t
ORIGIN      
        1 gtccacacat ccgtgtacgg cacctgcgcc gtcacaccaa aataccgccg cagatgctta
       61 tattcactct gcagctccac atactgccgg ttcagtgacg tatacgtatc caccagctgg
      121 tggtggggct tttccagttc accgcgctga tgtttctctt ctgccacccg ggcaaacagc
      181 gactgtaatt tcagataatc gctttcgttc ggtagctgcc actgactggc actgaaccag
      241 tgcgacacca tgtttttctt tcctgtggca tctgcaatct gttttgccgt tatccccagg
      301 gccgcgcgcg catcacgaaa gtaagaaatc agcggggcca tcacatgctg tttcagtgca
      361 ctgcccttcg ccgcataccc ggcatctttc ggacgatacg gcccctgata atgttccgcg
      421 aacagaatgc gctctgtggc ggggaaatac gcccgcaggc tttccttgtt gcatccgttc
      481 cagcgtccgg acggcttcgc ccagataata tggttcagca cactgaagcg ttcacgcatc
      541 atgatttcga tatcagatgc caggcgatga ccacagaaca ggtaaagact tccggcaggt
      601 ttcagcaccc gccagaactg cgccagacac tggtccagcc acttcaggta atcatcgtcg
      661 cccttccact ggttatccca gccctcaggc ttcactttaa agtacggcgg gtccgtgact
      721 atcaggtcaa cagaattttc gggtaacgac cggataaatt ccaggcagtc ggcgttgatt
      781 aactcacaac tggatatttt tacagtatta agcatggatc attaagcctg tctctgatag
      841 gctcattctg cttttgcgca aagcagtggg cctgaggttt gcttgtgaac ccaacgcatg
      901 agcagatggc tggtgggtgc ccctaacacc caccagccgc ccatttacca caaataaaaa
      961 agccttcact gcggaaggcg tctgtaacaa ccgaactgat agtctgccag acccgccata
     1021 accagctggg tcagtattaa ctggcagcgt tcgcgtgaaa ggtaagtatt ctgtgcaatc
     1081 tccccgactg tcgccggttc ggtaacgctt aattcattaa acaccactct ggcggtttct
     1141 gtcatatcct gctgttttag catgtctttt tcccttttcc ggttaacgtg acacaccaat
     1201 aactcttgtc gaaaaagcca gcaagctgaa agacaggtat tcaccgccac cagcgcgttt
     1261 actgtactga cgcgatttca gtcataaaaa acccgccagg cggcggggtg taaaaaatct
     1321 tctaacgtca ggcataaaac gcccatcgtt agagcaaatt taccacagat tcgggaaaaa
     1381 tcaacaacac tatcgcgtta ccctctttaa ctgccgctcc gcccatgcct cttcaatgtc
     1441 aaaccgaacc accaacgtat cgtaaaagcg tttcactgat tttttccacg tatcaagcgt
     1501 gatagcactc gtcactttgc atatggcatt aaatgcctcc gttgatggta gtctttcaca
     1561 gccacgacca ccacaacgct ggcagtctct gataacaggc ataccacgtt ttaccgactc
     1621 ttcacgatga atggcgacac cacgcccacg gcaatcctta caggcggtgg aaacctcacc
     1681 ctttccgcca cactccggac aggcaacttt taccacctcc ctgacttttt tccattcttc
     1741 ccagtaagac ggatacacac ctttcgtaca ctttgcccat accggcggct taccatccgg
     1801 atactggacc ttgtttgtaa aaactacgct ttcaataaat ttttccccat agcaacaagg
     1861 gcactgcttt ttactcgctg cgctgcgggc ataatcctca aaagcgtacg aagccataat
     1921 gcgcatcact accggtttta tttctgccgg gagttttctt aacgccgcca cgcgatcaca
     1981 ccgactgagt gcatattctg tcagcaattc tgttgcccgc tctctgtcat tcatactaat
     2041 gcccattttc ccaaggaacg cagaaaaccc catctcagcc cgattctgtg tcatgccctg
     2101 cgcggccatc acatcagtga tactcagcgc atctttcgac gttgaggccg atgcatcagt
     2161 caggccgggg gattttgggg agtagtattt cggtaaatct tccagtttca ttttttgacc
     2221 tgcccttcaa gcattatggg gtaaatcttc acccccagac gtccaccaga tactggctga
     2281 gcacgaacga tattgatttc atcaaactgc tcatcgtcca ttagcaaccc cgcatgcgtc
     2341 agcgcatcca gcggcgcttt cagaatattg tccaggtcac ggcggcgctt atccggtggt
     2401 tctgcaataa tttttattgc caaccttccg gacaggctta atttcagccg ctgctggcga
     2461 acaataagcg ccactgcccg gcgataacgc tccccggctt ttgatacaaa atatgtgctg
     2521 ccacgacgac gccagtaagt gttcaccgtt ggcgggtaag gcaaaacaaa ttctatgcgt
     2581 tcagtcattt atgctttcca cttcagaaca cccgaatttc tcgcgtgcat taaaaaacga
     2641 atcagcaaca acagctggct gccgtgtttt tcttcaaaat cttttacccc ggcgtgtagt
     2701 tcgctatggc atttacggca cagcggaata acaaacaaat catcagcctt tgttcccatc
     2761 cctcccagtc cataaccaat gatgtgatgc ggatcatctg cctgattgcc acacgtcatg
     2821 catttctgcg tttttaccca acgcgtgtat acaggcatct cttcccgttg tgatttctgg
     2881 cgctggagat actgagccgg tgactccgga tcaacggcaa tgctgaccac cgtcttttcc
     2941 tgtggcgggt tttgctggtg ggcgtgaggc agcggcgcaa gattttttgt gcgctgcttc
     3001 agtatgctgg tggcggtctg ctctcccggt acgatgtcgc tttcacggta cattgagcgg
     3061 attttttccg cacgcaaccc cagcgaacga cgtaataccg cttccggtag cgcgtccgcc
     3121 acctgattgc ggaccgccca ccaggataat tcagccagcg ataattcccg ttcctgcgag
     3181 ccattcattg catggcgtat gacgtcaatc atccatgcaa acaggttttg gtgagcaagt
     3241 tgcccgagtg attcggaggt ctggtcgcgc agctggttgt cgcagtgcca gcacaacacc
     3301 attgcgccgg taccataacg gtgaatgacg gtttcactgt ggtgataatc gccgtgtggc
     3361 cactggcagg atttaacatg gcgcagtaac cagtcagaca atgcgccagc gccaccagca
     3421 gcacgaatca ctcgttcgtc gctgaaaaat ggcagtaatg atttatcctc cgccagcggc
     3481 tggcgaacgg caggaacgac cccggacggc agattacgca tgcttttcgg ttccggctcc
     3541 accagtaccc gggtattgtg gaataccggc atggattcac ggcccggctt aacgatcacc
     3601 agcccgagtt ccggtaccag aacaggtcga agtaataccc gcacgttacc tccagatgcg
     3661 ttgctggaat gtgcgggacg gacgcggtgg gcgttcagag taaggaagcc tgacggagat
     3721 tatccagtga cgataatcga ggctgagggc tttcttaatc tcgtatccgc gtctgcggta
     3781 gttatgaatt agccattcgg cctgttcttc agtacatggt gggtgttggt accagtcggt
     3841 tttaaatgcg tgtgaacgcc gcccatgccg gatggcaagg tcggtatcag aattgtgaaa
     3901 tttggttttg tgcgccatct gttttctctg ctggcgcagc aggtgtcagg tgttcaggct
     3961 gacgtgcgaa ttgtaaacca gaatgccaga aaaaaacaaa acccgccgaa ccgggttaag
     4021 tgcgggtgcg ttgaggatgc ctgacacatc agaggtggcg agggatttct cccccgccag
     4081 gtctcttact cctcaggttc gtaagctgtg aagacagcga cctccgtctg gccggttcgg
     4141 attcgtacct cgcagaggtc tttcctcgtt accagtgccg tcactatgac ggttaaacag
     4201 atgacgatca gggcgattaa
//
LOCUS       DAJVOY010000244         4183 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVOY010000244 DAJVOY010000000
VERSION     DAJVOY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.247"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQL90_003893"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQL90_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1370498.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OQL90_003894"
     tRNA            complement(324..399)
                     /locus_tag="OQL90_003894"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OQL90_003895"
     tRNA            complement(406..480)
                     /locus_tag="OQL90_003895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OQL90_003896"
     tRNA            complement(597..681)
                     /locus_tag="OQL90_003896"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OQL90_003897"
     tRNA            complement(690..765)
                     /locus_tag="OQL90_003897"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OQL90_003898"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OQL90_003898"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1370499.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQL90_003899"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQL90_003899"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1370500.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQL90_003900"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQL90_003900"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1370501.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNVVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1040 c          932 g         1132 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gtaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc acattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DAJVOY010000245         4181 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVOY010000245 DAJVOY010000000
VERSION     DAJVOY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.248"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(42..212)
                     /locus_tag="OQL90_003901"
                     /pseudo
     CDS             complement(42..212)
                     /locus_tag="OQL90_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            522..2294
                     /gene="aspS"
                     /locus_tag="OQL90_003902"
     CDS             522..2294
                     /gene="aspS"
                     /locus_tag="OQL90_003902"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1370502.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2412..2864
                     /gene="nudB"
                     /locus_tag="OQL90_003903"
     CDS             2412..2864
                     /gene="nudB"
                     /locus_tag="OQL90_003903"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1370503.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2893..3633
                     /locus_tag="OQL90_003904"
     CDS             2893..3633
                     /locus_tag="OQL90_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1370504.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3668..>4181
                     /gene="ruvC"
                     /locus_tag="OQL90_003905"
     CDS             3668..>4181
                     /gene="ruvC"
                     /locus_tag="OQL90_003905"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1370505.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGR"
BASE COUNT         1033 a         1032 c         1179 g          937 t
ORIGIN      
        1 tcaaccggct gttttaatgc ttcggcgtaa tcggcagatc agccaactcg caccagaaac
       61 acgggctgac cgctggcgcg aaatttcgcc gccagcttcc cggcgcgatt aactacctca
      121 tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc
      181 aacgcagtgg ttttagcatt aagttcaagc atgctgactc ctgaaaaaac aaaatcgtgc
      241 ctcacacctt aatgcgatta gccgatggcg gaacgacaaa ttttgttaat ttttatgagt
      301 tcgggcaaca cctttatagc aaactggcgt agagtctgat ttcaggcccg atataaagtg
      361 gtaacgaggc gttccagcgc gggctaggct cttacggtgc ggcagaattt ccagtataat
      421 agccgccttt tttcattcag ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc
      481 agcagggggc atccgccggc agctaagtta agggatatct catgcgtaca gaatattgtg
      541 gacagctccg tttgtcccac gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc
      601 gtcgtgatct tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg
      661 tatttttcga tccggatcgt gcggacgcgt taaagctggc ctctgaactg cgtaatgagt
      721 tctgcattca ggtcacgggc accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata
      781 tggcgaccgg cgaaatcgaa gtgctggcgt cctcgctgac tatcatcaac cgcgcagatg
      841 ttctgccgct tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc
      901 tcgacctgcg tcgtccggaa atggctcagc gcctgaaaac ccgcgctaaa atcaccagcc
      961 tggtgcgccg ttttatggat gaccacggct tcctcgacat cgaaactccg atgctgacca
     1021 aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat
     1081 tctacgcact gccgcaatcc ccacagttgt tcaaacagct gctgatgatg tccggttttg
     1141 accgttacta tcagatcgtt aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc
     1201 ctgagtttac tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag
     1261 tgatggaagc gctggtgcgt catttgtggc tggaagtgaa gggcgtggat ctgggcgatt
     1321 tcccggtaat gacctttgcg gaagcagaac gccgttatgg ttctgataaa ccggatctgc
     1381 gtaacccgat ggaactgacc gacgttgccg atctgctgaa atctgttgag tttgcagtat
     1441 ttgcggggcc ggcgaacgat ccgaaaggcc gtgttgccgc tctacgtgtt ccgggtggcg
     1501 catcgctgac ccgtaagcag atcgacgaat acggtaactt cgttaaaatc tacggcgcga
     1561 aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg tctggaaggt atcaacagcc
     1621 cggtagcgaa gttccttaat gcagaaatca tcgaagccat cctggatcgt actgccgcgc
     1681 aagatggcga tatgattttc ttcggtgccg acaacaagaa aattgttgcc gacgcgatgg
     1741 gcgcactgcg cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac
     1801 cgctgtgggt tattgacttc ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa
     1861 tgcaccatcc gttcacctca ccgaaagaca tgactgctgc agaactgaaa gctgcaccgg
     1921 aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt
     1981 cagtacgtat ccataatggt gatatgcagc agacggtgtt tggtattctg ggtatcaacg
     2041 aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc cctgaaatac ggtactccgc
     2101 cgcacgcagg tctggcattc ggtcttgacc gtctgaccat gctgctgact ggcaccgaca
     2161 atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag
     2221 caccgagctt tgctaacccg actgcactgg ctgagctgag cattcaggtt gtgaagaagg
     2281 cagagaataa ctgatatgac tcaaatacac gaaatcattc gcgttgcatc aaggcggcaa
     2341 ttgagtgaac tcccatgagc atagataact atgtgaatgg gatgagcgaa ggcagtcaac
     2401 gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta gtggtcatct
     2461 atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc gatttctggc
     2521 agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag
     2581 taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt gacagtcagc
     2641 gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc
     2701 gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc gttttcactg
     2761 aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga
     2821 gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc agggctatct
     2881 ggagatattt ttatggcagg tcatagtaaa tgggccaaca ccagacatcg taaagctgcg
     2941 caggatgcta agcgcggtaa aatcttcact aaaatcattc gtgagctggt aaccgcggct
     3001 aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc gtgcggcggt ggataaagca
     3061 ctgtctaaca acatgacccg tgacacactg aaccgcgcaa ttgcacgtgg tgtgggcggt
     3121 gatgatgatg caaacatgga aaccatcatc tacgaaggtt acggtcctgg cggcacggca
     3181 atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt gcgtcatgca
     3241 ttcagcaaat gtggcggtaa cctcggtaca gatggttccg tagcctatct gttcagcaaa
     3301 aaaggcgtga tctccttcga gaaaggcgat gaagacacca tcatggaagc agctctggaa
     3361 gcaggtgctg aagacgttgt gacctatgat gacggcgcga ttgatgtcta cactgcatgg
     3421 gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag gtctgaaagc agacagcgcg
     3481 gaagtttcca tgatcccgtc caccaaagct gatatggatg cagaaaccgc accgaaactg
     3541 atgcgtctga tcgatatgct ggaagattgc gacgacgtgc aggaagttta ccataacggt
     3601 gaaatctctg atgagattgc agcgactctc tgatgaggcc tgctaaacag caaaacggag
     3661 acgcgtgatg gctattattc tcggcattga cccgggttcg cgcgtgaccg gctatggcgt
     3721 catccgccag gtaggcaggc aactgtccta cctgggtagc ggatgcatac gcaccaaggt
     3781 ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc gtgacggaaa tcatcaccca
     3841 gttccagcca gattatttcg ccattgaaca agtctttatg gccaaaaatg ccgactcggc
     3901 tctgaaactg gggcaggcgc gcggcgtggc gattgtggcg gcggtgaatc aggaattgcc
     3961 ggtatttgaa tatgcggcac gtcaggtaaa gcaaacggtg gtaggtattg gtagtgccga
     4021 aaaaagtcag gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta atccacaggc
     4081 ggatgccgcc gatgcgttag ctatcgcgat tacccattgt cacgtcagcc agaatgcaat
     4141 gcagatgagc gaatcgcggc tgaacctggc gagagggcga c
//
LOCUS       DAJVOY010000246         4083 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVOY010000246 DAJVOY010000000
VERSION     DAJVOY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.249"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            32..931
                     /gene="pgrR"
                     /locus_tag="OQL90_003906"
     CDS             32..931
                     /gene="pgrR"
                     /locus_tag="OQL90_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1370506.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1268..2881
                     /gene="mppA"
                     /locus_tag="OQL90_003907"
     CDS             1268..2881
                     /gene="mppA"
                     /locus_tag="OQL90_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1370507.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMKQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2932..3963)
                     /gene="ynaI"
                     /locus_tag="OQL90_003908"
     CDS             complement(2932..3963)
                     /gene="ynaI"
                     /locus_tag="OQL90_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1370508.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1147 a          896 c          960 g         1080 t
ORIGIN      
        1 aaatgattaa tagattgcat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat
       61 ggcgtttgtc gtcgttgcag aggagcgtag cttcactcgt gcagcagccc gcctgagcat
      121 ggcgcagtca gctttaagcc agatagtgcg tcgtatagaa gaacgattgg gattgcggct
      181 tctgacgcga accacgcgca gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt
      241 tcttggccct atgttgcatg acatagattc agccctggca tccctgagcg agctgcagaa
      301 ccgcccatcc gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt
      361 accagcaatg cgcacattcc tgaaatcgca tcctgaaatt gatattcagc tcaccattga
      421 ttatggtttg accgatgtcg tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga
      481 gatggataaa gatatgatcg ccattcgaat cgggccagat ataccaatgg ctattgttgg
      541 ctcaccggat tatttttctc gccgaagtgt tccaacgtca gtgtcacaat taatagatca
      601 tcaggcaatt aatttgtatc ttcccacatc gggtacagca aatcgctgga gattaatacg
      661 cggtggacgt gaagttcgtg ttcgcatgga aggtcagctt ttactgaata cgatagacct
      721 gatcattgat gctgcaattg atgggcatgg attggcgtat ctaccttatg atcaggttga
      781 gcgggctatt aaagaaaaaa aactgatacg tgttttggat aaattcacac cagatttacc
      841 cggttatcac ctgtactatc cacaccgtcg acatgctggc tcggcattct cattatttat
      901 agataggctg aagtataaag gtgctgttta gcactacttg ctgatacatt aatttaattt
      961 ctctcttaac gtattctcag ttcctttcaa cgttttggtt attttttatt cttcgtacaa
     1021 tggcgacaga tgctgatttt gataccgaaa actggtttga acgtgcgaag cctgaacgta
     1081 gtattcggtg ttctatgtgc tttgccgcat tttggcgtcg attatttaca ctaaatctga
     1141 tttaatatat tgatacataa aacattttat gcttcctgtg tcactttttt gatggaagtt
     1201 gtttgcattt ctttaaggcg aaacaaataa ttacgcatca attttaatgt cggttagagg
     1261 gaaacttatg aagcactctg tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc
     1321 tctttcgtat gctgcagaag ttccgagcgg cacagtactg gcagagaagc aggagctggt
     1381 gcgccacatt aaagatgagc ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga
     1441 gattcaggtc attcgcgatc tgtttgaagg actggtgaat cagaacgaga aaggggagat
     1501 tgtccccggc gttgcgactc agtggaaaag taatgacaac cgtatctgga cttttaccct
     1561 gcgcgataac gcaaaatggg cggatggcac accggtaacg gcgcaagatt ttgtctacag
     1621 ctggcagcgt ctggtggacc cgaaaacgct atcgccattt gcatggtttg ccgcgctggc
     1681 gggaatcaac aacgcacagg cgattattga tggtaaagct acgcctgacc agcttggcgt
     1741 caccgcagtt gatgcccata ccttgaaaat tcagcttgat aaaccgttgc cgtggtttgt
     1801 gaatttaacc gctaactttg ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa
     1861 agagtggacg aaacccggaa atctgatcgg caatggcgct tatgttctta aagaacgcgt
     1921 agtcaatgaa aaactggtcg tggtaccgaa tacccattat tgggataacg ccaaaacggt
     1981 actgcaaaaa gtgaccttcc tgccaattaa tcaggaatcc gcagccacta agcgttacct
     2041 cgcgggggat attgatatca ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa
     2101 ggatattccg gggcaggttt atacgccgcc gcagctcggg acctattatt atgcgtttaa
     2161 cacgcaaaaa gggccgacgg cagatcagcg cgttcgcctg gcattaagta tgacgataga
     2221 tcgccgcctg atgaccgaaa aagtgttggg aacgggcgaa aagccagcgt ggcattttac
     2281 accagatgtt accgccggat ttacgccgga accttcgccg tttgaacaaa tgagtcagga
     2341 agaactgaat gcgcaggcaa aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc
     2401 gctgaagctg acgcttttgt ataacacttc agaaaaccat caaaaaattg cgattgctgt
     2461 agcatcgatg tggaaaaaga accttggcgt agatgttaaa ttgcaaaatc aggaatggaa
     2521 aacctatatc gatagccgta acaccggcaa ttttgatgtt atccgcgcct catgggtggg
     2581 ggattataat gaaccctcca ctttcctgac attattaacg tcaacgcatt caggaaatat
     2641 ttcacgcttt aacaatccgg catacgacaa agttctggcc caggcatcga cggaaaatac
     2701 cgttaaagcg cgtaatgccg attacaacgc ggcagaaaaa atcctcatga agcaagcacc
     2761 gattgcacca atttatcaat ataccaatgg acgattaatc aagccgtggc tgaaaggtta
     2821 tcctattaat aacccggaag atgtggcgta cagccggatt atgtatattg tgaagcattg
     2881 atgtgatggg aactggcgtt acccttgtgg ataacgccag tgatgtctga tttagcgccc
     2941 ctgttccggc ggtgtaatgt tatccatata cagcgtctgg ctggggaagg caaagtccgc
     3001 gccgtgtgac tgcacaatat cgataatctt caaataaacg tcttgttgtg cagcaagcca
     3061 ttcagcccat accgtggttt tggtaaagca ataaaccata atattcaatg acgagtcggc
     3121 aaactggttg aaataaacca gtaaggtttg tcgctggtcg atggccgggt gatttttcag
     3181 catctcacgt acggcttcga caataacgcc cacttttgcc gcatcctcat aacgtaaacc
     3241 aatggtcgtg gtaatgcggc ggttggtcat tcgtcctggg ttttctacgc tgatcgacga
     3301 aaacagcgag ttcggtacgt acaatggacg attatcaaag gtcgtaattt tggtaattcg
     3361 ccagccaatt tccgctactg taccttcgat atttctgtcc ggtgaacgga tccagtcgcc
     3421 aatactgaaa ggacggtcga aatagagcat gatcccggaa aagaaattac tcagaatatc
     3481 tttaccggcc ataccgacag ccagaccacc aataccacca aaggtcagca agccagaaag
     3541 gctcatgccg aaatgttcgc cataaagcag aacaagcacc acaataatgg tgattttgat
     3601 aatgcgcgac ataatccgcg cactggtgat atcgcgacct tttttaatct gctgtttttc
     3661 aaactgatta atcagcaaaa atagcttaat cgtcagaata accgcaatca gggacgtaca
     3721 gataaaatcg ataacgcctg gggtgataaa tttgagttta tagttttcta taacataatt
     3781 aataatgcta ccaacagcac tgataattat ggtgtagatt aaaaattgca ccgcatggaa
     3841 taaaaaacct tttcttttac gatttccgcg gcgaaaccaa aagctcatca gaatcaatgc
     3901 tgcgcagcta ccgaaaataa tgaccagatt aagcgcatta tttgtaaaca gttcagcgat
     3961 cattgtttta tcaggctcct ccagataatt gtcgtcatgc cggaaccccc tggcggggct
     4021 attttaccgc gacaattcat tcagatcatc aatagtcagg gaaggaagta gcaacattag
     4081 cta
//
LOCUS       DAJVOY010000247         4078 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVOY010000247 DAJVOY010000000
VERSION     DAJVOY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.250"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..1046)
                     /locus_tag="OQL90_003909"
     CDS             complement(<1..1046)
                     /locus_tag="OQL90_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1370509.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1647
                     /locus_tag="OQL90_003910"
     CDS             1537..1647
                     /locus_tag="OQL90_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS1370510.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="OQL90_003911"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="OQL90_003911"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1370511.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..3915)
                     /locus_tag="OQL90_003912"
                     /pseudo
     CDS             complement(2963..3915)
                     /locus_tag="OQL90_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1093 a          829 c          957 g         1199 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgactaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagtgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcatctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggca gcacgaacgg
     3421 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
     3481 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
     3541 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
     3601 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
     3661 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa
     3721 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
     3781 taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa
     3841 tttagcggca ttgttaaacg aactggactc tgccagtatg acaaatgcct tcatacttga
     3901 taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggccgt
     3961 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
     4021 gctaaagtca ttagagatgc tttgccctta atgtaaccat atcgcaataa gggtaatg
//
LOCUS       DAJVOY010000248         4064 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVOY010000248 DAJVOY010000000
VERSION     DAJVOY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.251"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(188..655)
                     /locus_tag="OQL90_003913"
     CDS             complement(188..655)
                     /locus_tag="OQL90_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370512.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
     gene            complement(1723..1914)
                     /locus_tag="OQL90_003914"
     CDS             complement(1723..1914)
                     /locus_tag="OQL90_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1370513.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            1982..2305
                     /locus_tag="OQL90_003915"
     CDS             1982..2305
                     /locus_tag="OQL90_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCS1370514.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            2268..3011
                     /locus_tag="OQL90_003916"
                     /pseudo
     CDS             2268..3011
                     /locus_tag="OQL90_003916"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2983..3543
                     /locus_tag="OQL90_003917"
     CDS             2983..3543
                     /locus_tag="OQL90_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCS1370515.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            3550..3783
                     /locus_tag="OQL90_003918"
     CDS             3550..3783
                     /locus_tag="OQL90_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1370516.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
BASE COUNT          970 a          934 c         1227 g          933 t
ORIGIN      
        1 aaaaaaacag tgtcatgagg tttacgcagg caaaaccagc gttattcaca tggctgaata
       61 gctatgcata cggttaaaat ttatcaggtg cgatcgcggc agcttttcgg gtggtttgtt
      121 gctattttta cctgtcagca gccgtgatcg cgctgaacgc atttcagcgg tgcgtacaat
      181 taagatatta cgttaaattg tgagggaatg gtccccatat actgacttta tcatagggat
      241 agggttggta gtgggtcgat tgggtttcgg cattgtttcc gttaatgcca attgcatttt
      301 tgtatttgat gcaacagaag gcgtgagatt ctcctgggcc tggaaaggct aagtgtttag
      361 aattgatggc aaaatatctt ccgtccgatt tttgaagtag ttgttgtttg gctgtctccc
      421 aaagggtgct gttacaaact tcttgaattt gtagtttttg cagggctctt attactgcgt
      481 tgtcatgttc caattcttta ggggagtgta ttattctctc ttttaataag ctgtttacca
      541 cagggtttag ttgttgtcta aagcacgcag ataatgccat tgctacacaa ttttgttccc
      601 gaggaagcat gtttggcggg gtatgccgtg tttgatgtga tatgtctatt ctcatagaag
      661 gtctctggct tatagcatca acaaatacgg ctataaaaca aaattttcat agagtgattc
      721 aagatcttct tgagatcctt ttttgcggcg cgtaatctgc tgcctgcaaa caaaaaaacc
      781 accgctacca acggtggttt gtttgccgga atctagagct accaactctt tcttccgaag
      841 gtaactggct tggtggagca cagataccaa atactgtcct tctagtgtag ccgcagttag
      901 gccaccactt caagaactct taatatctca atccaccttg tctagttacc aatggctgct
      961 gccagtggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1021 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1081 tacaccgaac tgagatacca acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1141 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1201 ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac ctctgacttg
     1261 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccaccggcg
     1321 cggccttttt cgggctcttg ttttttgctc acatgtttct tttggcttta tcccctgatt
     1381 ctgtggataa ctgtgttacc gtctttgagt gcgctgatac cgctcgccgc agccgaacga
     1441 ccgagcgtag cgagtcagtg agcgaggaag cggaagagcg ccggaagcta cattttctcc
     1501 ttacgcatct gtgcggcatt tcacacccgg catggtgcac ttttcataca atccgcactg
     1561 atgccgcata agtaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     1621 gccccgacac ccgctaaaac ctgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     1681 cgctcacagg ccagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccatc
     1741 atccccgaag cgtgcgaggc agctgcggta aagctcgtca gcgtggtcgt gaagcgcttc
     1801 gcagatgtca gcctggtcgt cggcgtccag ttcattcagt ttttccagca gggtcagcgt
     1861 ctggcttctg atgaagcggg ccatgttaag ggcggttttt tcctgtttag tcacagttct
     1921 gtttctccat gtgtggggag tctgtcggag gtttttaatg ataccggtga aacgcgaaag
     1981 gatgctcacg atacgggtca cggatgagga acatgcgcgg ctgctggcac gttgtgaggg
     2041 aaaacaactt gcgtcatgga tgcgtaaggt ctgcctgggg gcaccgccat cgaaaacgtc
     2101 aggtcttccg acactggcac cgccgctgtt gcgccagttc gcttcggtcg gcaataacct
     2161 gaatcagata gcccggaaga taaacagcgg acagtggtcg gggcatgacc gggttcatgt
     2221 ggtggctgca ctgatggcca tcggacgtga actgagtgag ctgagggatg aggtccgtaa
     2281 acagggagag cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     2341 cggttgatta cctgctgggc cgggagcgta accgggaagg ggcgagagtt ctgcggggtg
     2401 ctcccgaaga ggtgcgggaa ctcatcgatg ccacgccgtt tgcgaaaaaa tacacctcgg
     2461 gggttctgtc ttttgcagag cagaccctgc caccgggaga gcgtgaaagg gtgatggaga
     2521 gctttgagcg ggtactgatg cccggtcttg aaaaaaatca gtacagcatc ctgtgggtgg
     2581 agcaccagga caaggggcgt cttgagctga attttgtcat cccgaatatg gagctggcga
     2641 gcggaaaacg gctccagccc tattacgacc gagcggaccg tccgcgaatc aatgcctggc
     2701 agacactggt gaatcaccat tacgggctgc acgacccgaa cgcaccggag aacaggcgga
     2761 cacttgtcac cccgaataat ctgccgaaag cgaaacagga ggccgcagaa gcgattacga
     2821 ggggactgga ggctctttac catgccggag cgctaaaaac gcgtcaggat gtcactgagg
     2881 cgcttacagc ggcaggtttt gaggttgtga ggacaaccag gagcagtatc agtatcgccg
     2941 acccggaggg cgggcgtaat ctcaggctga aaggggcgct gtatgagcaa tcttttacaa
     3001 atggggacag acttcgagaa gaaactgaaa gagcgtgccg tctctacaga gaacatactg
     3061 aacagcgagt tcaggaagct cgaagcgtct gtagacaggg aactgaactt aaacgggcag
     3121 aaaatcagag acgctatcag cgcacacaca gcagcggtga aagaacaact ggagacgctg
     3181 aacagtgccg tgaacgcgca gttcagcgcc acagagaacg aactggcccg gcggcaggaa
     3241 gagctgctct ggaagctggt caaagggcgg atactgtacc cgtcgctgac ggcgctttgc
     3301 gtcacgggcg ggatttttct ggcgtcatgg gggctgattc agtggcagga aagccggatt
     3361 gcggcgaaca tcctggctat cagggaccag gaagagacgc tggcgaaact ccgggaaaaa
     3421 acatggggtg tgacgtatca cgagggcagg aacgggaaat ttctggtatt gccgtccggg
     3481 gtgaaggggg aaaacaactg gacggtggag aagaagaacg cagtaagact ggtcagggag
     3541 taagtgagta tgacagagct ggaaatgcac ttactgaacg ccttagagca attgcagcag
     3601 gactatacgc agcggctgaa agactgggag agcgccttca cggatttgca gcatatgttc
     3661 gagctgacac gacaggagaa cgggcaactg agggaacagt gcgacgactt gagtcagcag
     3721 gtgcagagat tagtcgagca ggtggaccac ttgagtcggt tatttatcac gaacagcaga
     3781 taagggatga acaggaaagg caggcacgcc tggaacggga gcgtgcgcgg tcctggcatg
     3841 aagagcagga acgggaagca cgggtatggc gtggcccgtc aatagggata tgacggtgag
     3901 ttgttccggg aggttgccag atggtaccgg aacgcgttaa aaacgcatcc gggcgcgatt
     3961 ctggcgtgtc gtttttgttg ctgtgttttg cctctttgcc agatcgcgct cattcctcag
     4021 cctcatcccg cgatgtctgc gtatgtcatc aactgaaaag atgg
//
LOCUS       DAJVOY010000249         4005 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVOY010000249 DAJVOY010000000
VERSION     DAJVOY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.252"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..202
                     /locus_tag="OQL90_003919"
     CDS             <1..202
                     /locus_tag="OQL90_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1370517.1"
                     /translation="IAKSFVKTIKCDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSA
                     LDYRSPREYLRQRANDNRCLEI"
     gene            complement(764..1711)
                     /gene="icsP"
                     /locus_tag="OQL90_003920"
     CDS             complement(764..1711)
                     /gene="icsP"
                     /locus_tag="OQL90_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCS1370518.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGTHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3092..3244)
                     /locus_tag="OQL90_003921"
                     /pseudo
     CDS             complement(3092..3244)
                     /locus_tag="OQL90_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3300..3992
                     /gene="ospZ"
                     /locus_tag="OQL90_003922"
     CDS             3300..3992
                     /gene="ospZ"
                     /locus_tag="OQL90_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCS1370519.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
BASE COUNT         1248 a          761 c          745 g         1251 t
ORIGIN      
        1 aatagcaaag agcttcgtga aaacaataaa gtgtgactac atcagtatca tgcccaaacc
       61 tgacgggtta acggcagcaa agaaccttgc agaggcattc gagcattata acgaatggca
      121 tccgcatagt gcgctggatt atcgctcgcc acgggaatat ctgcggcagc gggccaatga
      181 taacaggtgt ctggaaatat aggggcaaag ccacccacga acgatccagc atataatcaa
      241 gaaattagat ttcatttaac ataagataga ttatgtgcac caagaggcat ttccttaatt
      301 tctgaattta caggctcttt tgctctcttt gctatgctgt ggatttggaa agatagagtg
      361 ctggctgatt atttaggtat tttcatcagc atgtccctga tagcactgtt ccatcagcgc
      421 tttcaggcga taatagtaga ccttgggtag cctctgctca tgtgtaactt gataccccga
      481 gaacagccac gatgatctaa gcggttctta tttctccggt ctcagggtcg gtaatgccaa
      541 cagtaagtcc acagtaatca acgaagagtt ttttgaaccg ccccggaaat cctggagact
      601 aaacgccctg agaaagaggt aaacagaatg actaaaaata cacgtttttc ccccgaagtc
      661 cgtcaacggg cagttcgtat ggttctggaa agtcagggcg aatatgactc acaatgggcg
      721 gcaattttta gccatgaaaa cgtaatcaac tcggtgaacc aattcaaaaa atatacttta
      781 tacctgcgga agtgagaaaa ccaatatatt caatacttgc actattttta atggtgccag
      841 atatattggt actttgctca taaagagatg tatcaccttt tttattagaa atgtaatttc
      901 tggagccttc tatataaaat tttgcactgg gggtgacata gtatccaata ttcgcagcaa
      961 cagagcagta attttggttt tttatttcat ctttaaaaat tgtctcagtc tgatagtgtt
     1021 tatctgtatc agatgaacga acccaaccac tatatttcag ttctgctcca aactcaaaat
     1081 tctcatggcg gtaattagca gttaatccaa tataaggtat tttgaagagc tgtttgtaac
     1141 ctattgttct ttcaccacta ggatgtgtcc cttttttatt cctgttcccc ccgttttcac
     1201 tataaatata actccctccc attgcattaa aactgtaacg actctcttgg taacctgcta
     1261 ttagtccaag tcgataatcc aaattattta ataaccaacc tttaagattc agctcgaatt
     1321 cgttagcatc acggagatgc gtattgggat gccagctctg gtcggtccat acctgagggg
     1381 tattggaatt gttccagtct ttatcaacca tgcttgcttt ctggttacct aaagtagtcc
     1441 aaccggaaac tccgaaagac acttttggaa ttagcttcca atcgatgcca cctctaacaa
     1501 tagtcgcatt actgtatttc cagtccagtt gactaatttt tctcccccct tccttgggat
     1561 gataaacgcg ttcttttgtt ttgccactca gagatcctag actaatatcc gtactgatgt
     1621 tatccggtat gaaaagtgga tagttagtgg tagcatgtgt agtaaagatc gcaggtacac
     1681 aaagtgcaag tacaaggaat tttaatttca tcgagaactc tacttttttg gttgaaatgt
     1741 ccatgatctt cttactttat aagataaacg tcaatgccaa gcattatttt aattaaatat
     1801 ttgtttatgt taccaagaac atatctttta ttataatatt tattgaactt ttgtaaaaat
     1861 aaaaaaatac ctcattgtgc gaataaagta acgggggcca tagatttctc tacctacctt
     1921 ttggtgttca tacctgaggc tgatagtttc aggattccgc gacaattcta ctcaccttct
     1981 tgttctctcg ccaatatgaa atcaatcaga aaagcgttcc ataagaacaa cgataagaca
     2041 ataaattgtc atcacggctg caatagccaa cgtgcctttc agtaccaaca tgacaacctc
     2101 ctgtatttga cgtaacgtgt gctgataaat atgtatttgt atctcaactg actcaataca
     2161 acacttaagt atagtttttc agattttgtt tttcccgtga aatattatgg aaatggcaga
     2221 agcaatacat tgaattccga ttcgagtgaa tacggctagc gggcacctca ctttagcact
     2281 gaagccatca acttttattt gacttatttc aatggaaagc tttctcacta tcagtacagg
     2341 taattcccat ccttttaaag tcgagagtca gactgcgact aatacagtta aagtgaccaa
     2401 actcagttat cctctacaca ttttacctca tgtttacttg gacaattcat tggtgctttt
     2461 ataaaagaat aaatattcta ctaatttaat gcatcaccta aaatagtatt ttttataata
     2521 gaactgtatc tatagtatat tattaaagct taattattta ttatgcttta atgtggttgt
     2581 ccgattaagg actaatcctt gaaaacgccg tatagggcgg gagttcactc tattgcgata
     2641 acgtaaccgt acaatattgt gcgtttccca aattgaccac tactgaacta ttctgggatt
     2701 taaatgcaat aaaatggttg gttgaaggtc gtggtgatga tgttagtcct tttgagaata
     2761 ttccagtgcc agatcttact tataataaag aggcttggca gtttggataa gctattgtcc
     2821 ttattccact aatacaaact ttttgtaaag tatattaaat atacttcatt tcatactgaa
     2881 atccccggaa gtattcaatc aaacaattac accataaaag gaagagggag ccccaattaa
     2941 gttatcaagc atgtttatga tacctgcagg gtataaattt tctggctgca agcagacaca
     3001 tataaaacgt ctttattgat cctacccaca taatatggac gcagtcgtaa gtgaggctct
     3061 gtgagcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
     3121 ccggactgag gccaccacac caacggtgcc gtcgccagcg attgtaatcg cactcgatat
     3181 aattaaacac tgttgtccgc actatttccc gatagataaa gtgttctccg tggagacatt
     3241 ccacttagga acaattatat tgttcatggt aatgtttata tttgagtata gagattaaaa
     3301 tgattagtcc catcaagaat attaagaacg tatttccaat aaatactgca aatactgaat
     3361 atatagtgag aaatatttat cctagagttg aacatggtta ctttaatgaa tcaccaaaca
     3421 tatatgacaa gaagtatatc tctggtataa ccagaagtat ggcacaatta aaaatagagg
     3481 agtttattaa tgagaaatcc agacgactta attatatgaa gacaatgtat tctccatgtc
     3541 cggaagattt tcagcctatt tccagagacg aagccagtat accggaagga agctggttga
     3601 cagttatatc tggaaaacgt cctatggggc aattttctgt ggatagtttg taccatcctg
     3661 acttacatgc attatgtgag ctcccagaga ttagttgtaa gatcttccct aaagaaaata
     3721 gtgatttttt gtatataatt gttgtattca gaaatgattc tccccaagga gaactacggg
     3781 caaatcggtt tatagaacta tatgatataa aaagagagat tatgcaagtg ttaagagatg
     3841 agtcgccaga gttaaagtct attaaatctg agattattat tgctcgtgaa atgggagaac
     3901 tttttagtta tgcatcagag gaaatagata gctacataaa acaaatgaat gacaggtttt
     3961 ctcaaatcaa ggctcgaatg tctgttactt aatgtatatt aattg
//
LOCUS       DAJVOY010000250         3975 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVOY010000250 DAJVOY010000000
VERSION     DAJVOY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.253"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            7..3480
                     /locus_tag="OQL90_003923"
     CDS             7..3480
                     /locus_tag="OQL90_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1370520.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTLGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            3548..>3975
                     /locus_tag="OQL90_003924"
     CDS             3548..>3975
                     /locus_tag="OQL90_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1370521.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTH"
BASE COUNT          932 a         1025 c         1217 g          801 t
ORIGIN      
        1 gatgtaatgg gcaaaggtgg cggcagggcg cacacaccgc gtgaggcgaa agacaatctc
       61 aaatccacgc agatgatgag cgtgattgat gcgattggtg agggaccgat agaaggcccg
      121 gtgaaaggcc tgcagagtat tctggtgaac aaaaccccgc tgacggacac ggacggtaat
      181 cccgtgatac acggtgtgac cgcggtctgg cgtgccgggg agcaggagca gacaccaccg
      241 gaaagctttg agtcctccgg ctctgaaact gtactgggtg tcgaagtgac cagggcaaaa
      301 ccggtaacac gcaccattac gtcagcgaac attgaccgcc tgcgggtgac cttcggggtg
      361 cagtcactgg tggagaccac gtcaaagggt gaccgtaatc cgtcctctgt ccgtctgctg
      421 attcagttac agcgtaacgg taactgggtg acagaaaagg atgtcaccat taacggcaag
      481 accacctcac agttccttgc gtcggtgatt ctggataatc tgcctccccg cccctttaac
      541 atccggatgg tcagggagac ggcggacagc accacggacc agctgcagaa cagaacgctg
      601 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggccattgtg
      661 gggatgcagg tggatgcgga gcagtttggt ggtcagcaga tgacggtgaa ctaccatatc
      721 cgcggtcgca tcatccaggt gccgtcaaac tatgacccgg aaaaacgcac gtacagtggt
      781 atctgggacg gcagtctgaa accggcatac agcaacaacc cggcctggtg cctgtgggac
      841 atgctgactc acccgcgcta cggcatggga aaatgtctgg gggcggcgga tgtggacaag
      901 tgggcgctgt atgccatcgg gcagtactgc gaccagacgg tcccggatgg tttcgggggg
      961 accgagccgc ggatgacctt taatgcgtac ctggcacaac agcgtaaggc gtgggacgtt
     1021 ctcagtgatt tctgctctgc gatgcgctgt atgccggtat ggaacggtca gacgctgacg
     1081 ttcgttcagg accgcccgtc ggatgtggtg tggccgtaca ccaacagcga tgtggtggtg
     1141 gatgataacg gcgtgggatt ccgctacagc ttcagtgccc tgaaggaccg gcacacggcg
     1201 gtggaggtga attacaccga cccgcagaac ggctggcaga cctccacgga actggtggaa
     1261 gacccggaag ccatactgcg ctacggacgc aacctgctga agatggatgc gtttggctgt
     1321 acctgccgcg gtcaggctca ccgtgcaggt ctgtgggtga taaagaccgg actgctggaa
     1381 acgcagacgg tggacttcac gctcgggtca caggggctgc gtcacacacc gggtgacatt
     1441 attgaaatct gtgataacga ctatgctggg actatgaccg gcggacgtgt cctgtccatt
     1501 gatgctgcca cccgcaccct gacgctggac cgtgaggtta ccctgccgga gacaggtaca
     1561 tcggcggtga acctgattaa cggcagtggt aagccggtga gtgtggacat caccgcacac
     1621 cccgcgccgg accggataca ggtcagtacc ctgcctgatg gtgtggagac atacggggtg
     1681 tggggactct ccctgccgtc actgcgccgt cgcctgttcc gctgtgtctc cgtccgggaa
     1741 aacacggatg gcacctttgc catcacggcg gtgcagcacg taccggaaaa agaagccatc
     1801 gtggataacg gtgcccgctt tgagccgcag tcaggctccc tgaacagcgt catcccaccg
     1861 gcagtgcagc acctgacggt ggaggtgagc gcagctgacg gccagtatct ggcacaggcg
     1921 aaatgggaca cgccgcgggt ggtgaagggg gtgcgcttca gtctgcgact gaccagcgga
     1981 agcggagaag acagccgtct ggtgaccacc gctatcactg cggatacaga gcatcgtttc
     2041 agtggtctgc cgctcgggga atacaccctg acagtcaggg caattaacag ttatggccag
     2101 cagggcgaac cggccaccac caccttccgg attaacgcgc cagcaaaacc cgccaccatt
     2161 gaactgacgc tggggtattt tcagataaca gcggtaccgc gtcttgcggt gtatgacccg
     2221 acggtacagt ttgagttctg gttttcggag gcaaaaatcg cagacacatc tcaggtggaa
     2281 acctctgccc gttatctggg gaccggcagt cagtggagtg tatccggccc gcacattaag
     2341 cccgggaagg atttctggtt ttacgtgcgc agcgtcaacc tggtggggaa atctgctttt
     2401 gtggaggcca gtggacgggc cagcaatgat gcagaagggt atctggggct gtttcgggaa
     2461 aaaataggaa aactgcatct ggctcagggg ctgtgggagc tgatagacaa cagccagctt
     2521 gcggatgaga tggcggagat gaagaccacc atcaccgaaa cccgcaatga aatcacacag
     2581 acggtcagta aaacgctgga ggaccagagc gccaccatac agcagataca gcgcgtgcag
     2641 aaggacacaa atgatgacct tgctgcactt tacatgctga aggtacagaa aacaaaaaat
     2701 ggcataccct atgttgccgg tattggagcg gggattgagg atactgatgg ccagcccctg
     2761 agcaacatac tgctgctggc tgaccgtatt gcgatgatta acccggagga cggcaacacc
     2821 acgccgttat ttgtggcgca ggggaatcag ttgttcatga acgatgtgtt cctgaagcgg
     2881 ctgtttgcgg tgagtatcac ctcgtccggc aatcccccga cgttttccct gacgccggag
     2941 ggcaggctga ccgcaagaaa tgctgatatc agcggtaacg tgaatgcgaa ctccgggacg
     3001 ctcaacaacg tcacgattaa cgagaactgc cgggttctgg gaaaactgtc cgcgaaccag
     3061 attgaaggcg atctcgttaa aacagtgggt aaggcttttc cgcgggactc ccgggcaccg
     3121 gagcggtggc catcagggac cgttaccgtc aggatttatg acgatcagcc ttttgaccgg
     3181 caaattgtta ttccggcggt ggcattcagc ggcgctaagc atgagagaga gcatactgat
     3241 atttactcct catgccgtct gatagtgcgg aaaaacggtg ctgaaattta taaccgtacc
     3301 gcgctggata atacgctgat ttacagttgc gttattgata tgcctgccgg tcacggtcac
     3361 atgacgctgg agttttcggt atcagcatgg ctggtgaata actggtatcc cacagcaagt
     3421 atcagcgatt tgctggttgt ggtgatgaag aaagccaccg caggcatcag tatcagctga
     3481 attttataac ccatatacgg gcgccagaaa tggcgccttt tttattgcag aaaagcgaga
     3541 ggtaattatg cgtaaagttt gtgcagccat tttgtccgca gccatctgcc tgtccgtatc
     3601 cggtgcgcct gcatgggcgt ctgaacatca gtccacactg agcgcggggt atcttcatgc
     3661 ccgtacgaac gctcccggca gcgataatct gaacgggatt aacgtgaaat accgttatga
     3721 gtttacggac gcgctggggc tgattacgtc cttcagttat gccaatgctg aagatgagaa
     3781 aaaaacgcac tacagcgata cccgctggca tgaggattcc gttcgtaacc gctggttcag
     3841 cgtaatggcg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg cgatggcggg
     3901 tgtggcttac agccgtgtgt cgactttctc cggggattat ctccgcgtaa ctgacaacaa
     3961 ggggaaaacg cacga
//
LOCUS       DAJVOY010000251         3895 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVOY010000251 DAJVOY010000000
VERSION     DAJVOY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.254"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            124..627
                     /gene="yeaK"
                     /locus_tag="OQL90_003925"
     CDS             124..627
                     /gene="yeaK"
                     /locus_tag="OQL90_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCS1370522.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(628..732)
                     /locus_tag="OQL90_003926"
     CDS             complement(628..732)
                     /locus_tag="OQL90_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370523.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            901..1347
                     /locus_tag="OQL90_003927"
     CDS             901..1347
                     /locus_tag="OQL90_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCS1370524.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1304..2125)
                     /gene="nimR"
                     /locus_tag="OQL90_003928"
     CDS             complement(1304..2125)
                     /gene="nimR"
                     /locus_tag="OQL90_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCS1370525.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2222..3418
                     /gene="nimT"
                     /locus_tag="OQL90_003929"
     CDS             2222..3418
                     /gene="nimT"
                     /locus_tag="OQL90_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCS1370526.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3458..3805
                     /locus_tag="OQL90_003930"
     CDS             3458..3805
                     /locus_tag="OQL90_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCS1370527.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3827..>3895)
                     /gene="yoaF"
                     /locus_tag="OQL90_003931"
     CDS             complement(3827..>3895)
                     /gene="yoaF"
                     /locus_tag="OQL90_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCS1370528.1"
                     /translation="LPGGETIDEWALWRRDHPAGEK"
BASE COUNT          895 a          996 c          976 g         1028 t
ORIGIN      
        1 acacctcagt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
       61 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt
      121 gaaatgactg aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca
      181 caagaaggtg ctaacttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
      241 tcagaaattc gtggcaccgc tttaggccag ggagctaagg cactggtatg taaagtcaaa
      301 ggcaatggcg taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg
      361 agccaacttg ccagccatat aggcggatta cgcgcttcgt tagccagccc tgctgaagtc
      421 gatgaactca caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc
      481 aaactggttg ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggg
      541 atgctggata aatccgttat tttgaaaacc acggattatt tgcgcattgc gcaaccagaa
      601 ctggtcaatt tccgccgcac tgcgtaacta gcctgtccgt tcaataagca gaacggacaa
      661 aaccagcaca acagcaatgg caaaaaacga tgacgtgata atcagtgtct cgacgaacat
      721 ttgatcgttc atagcatgcc cccgggagat agctgttttc acgttattgt tagcgtgcac
      781 aaatggcagt ttgatgacag ttcgcctgat ttttatgcaa aaaacgtaaa gattttctac
      841 ttccttcctg cagcaagcgt aaagtaagca ggcttattat tttttggcaa ggaaaccacg
      901 atgtttgatg tcactttgct gatcctgctc gggttagctg cgctgggctt tatcagtcat
      961 aacaccactg tcgccgtttc aattctggtg ttgatcattg tccgcgtcac accgttaagc
     1021 acctttttcc cgtggattga aaaacagggg ctaagtatcg ggataatcat tctgacgatt
     1081 ggcgttatgg cccccatcgc cagcgggacg ctaccgccct caaccttgat tcactcgttt
     1141 ctgaactgga aatcgctggt ggcaattgca gtaggcgtga ttgtctcctg gctgggtggg
     1201 cgcggcgtga ccttaatggg cagccagccg caactggtcg ccgggctgct ggtaggcact
     1261 gttttaggcg tggcgctgtt tcgcggcgta ccggtcggcc cgcttattgc cgcgggtctg
     1321 gtttcgctga ttgtggggaa acagtagtta atcccgcaat atatcggcct ggcgtttgcc
     1381 ccaggccttt cttaaacata gtaataaagg ccgttgttga atcatacccc agcgtatgag
     1441 caactttctg tacagtatcg cctttcacta atccctgaag tgccataatc agctgtaact
     1501 gctgacgcca ctggcgaaaa ctcaaccccg tctcttttac gatcagtcgc gcaagattgc
     1561 gttcactcat cgcaaaaaag ccagcccatt gccctaatga cccccactcg acgggccctt
     1621 tcgccatcat ctccaccatc gtgcggattt taggatgaga agaaacgggc aggtgcagct
     1681 gttgttgtgg ctgctgaggg agttcatcaa acaggacctg tattaatctg cgggtcatcg
     1741 gctcagcacg ctgagtcgtc gtcctgtttg ccagggttaa aatcaattcc cggcataagg
     1801 gcgatatttt caatgtgcaa catgttgtcg gcattgtcac tgcgctgggt tcgataaaca
     1861 gaaaacagag ttcagcgtta gctgtcactt gattgctatg ttccaccccg cccggtatcc
     1921 aaaccgcata ttgcggcggc accatccaca aagcattttc caccgtacag gtaattgcac
     1981 catgtaatgc tagtatcagc tgtcctttgc ggtgctgatg tacaggactg gttaactcat
     2041 ccgttccggc gtgaatacaa aatgcgaccg cggcttcatg gtgtcggtcg ggttcatagc
     2101 cattgagatt caacctgtgc atcattttgt ccgaatttag cgataatttg tcattttagc
     2161 ttgattcaac ataacaataa aaacggtaag gtacagcctc gtttgtaaca atgagaagca
     2221 tatgacctgt tcaacttcat taagcggcaa aaacaggatt gtccttatcg ctggcattct
     2281 gatgattgcc acaacattac gcgtcacctt taccggcgca gcaccgttac tggatacgat
     2341 tcgttccgct tactcgctga cgacagcgca aaccggctta ttgaccaccc tgccattatt
     2401 ggcctttgcg ctaatctcac ctttggccgc cccggtagcg cgacgttttg gtatggaacg
     2461 tagcctgttt gccgcgttac ttttgatctg tgctggtatc gcaattcgct ctctcccttc
     2521 gccttactta ttatttggcg gtacagcggt cattggcggt gggattgcat taggcaatgt
     2581 cttactgcca ggattaatta aacgcgattt ccctcattcc gtcgccagac ttaccggcgc
     2641 atattccctg acaatgggag ctgcggcggc acttggatcg gctatggtag tgccgctggc
     2701 tttgaacggt tttggctggc aaggcgcgtt gctcatgctg atgtgttttc ctctgctggc
     2761 tcttttttta tggctgccac agtggcgaag ccaacaacat gcaaatttga gtacctcgcg
     2821 cgccttacat actcggggta tctggcgttc gccgcttgcc tggcaggtca cattgtttct
     2881 tgggagcaac tcactggtct attacgtgat tattggctgg cttccggcga tcctcatcag
     2941 tcacggctat agcgaagcac aggcgggttc actgcatggt ttgctgcaac tagccacagc
     3001 agctcccggt ttgctgatcc ctcttttctt acatcatgtg aaagaccagc gtggtattgc
     3061 agcgttcgtt gccttgatgt gcgcagtggg cgctgttggg ctctgcttta tgccagcgca
     3121 cgcgatcacc tggactctgc ttttcggttt tggttccggc gcaacaatga tactggggtt
     3181 gacgttcatt ggtcttcggg caagttctgc gcatcaggcg gcggcactct cggggatggc
     3241 acaatccgtc gggtatttgc tggcagcctg tgggccgccg ctaatgggta aaatacacga
     3301 tgctaacggt aactggtccg taccactgat gggtgttgca atactttcac tactgatggc
     3361 gattttcgga ctttgcgccg ggagagacaa agaaattcgc tcatatccgg tgctatagtg
     3421 acgtgacaaa tcatgcgtga aagggagaac aaacacgatg aatattcagt gcaaacgcgt
     3481 ttatgatccg gctgaacaga gcgatggtta tcgcgtactg gtcgaccgcc tctggccgcg
     3541 cggtatcaaa aaaaccgatt tagcccttga tgagtgggat aaagaaatca cgccgtcaac
     3601 ggaactgcgc aaagcctttc acggcgaagt cgtcgatttt acaacctttc gcgagcaata
     3661 tcttgcagaa ctggcgcaac acgagcaaga aggaaagcgg ctggcggaca tcgccaaaaa
     3721 acagccgctg accctgctct acgcagcaaa aaacaccacg cagaaccatg cgctggtgct
     3781 ggccgactgg ctacgtagct tgtgatttta gtacagcatc cggcggttat ttttcaccag
     3841 ccggatggtc ccgtcgccac aatgcccatt catcaatcgt ttcaccgccc ggtaa
//
LOCUS       DAJVOY010000252         3861 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVOY010000252 DAJVOY010000000
VERSION     DAJVOY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.255"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(37..594)
                     /gene="ymfL"
                     /locus_tag="OQL90_003932"
     CDS             complement(37..594)
                     /gene="ymfL"
                     /locus_tag="OQL90_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCS1370529.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDIEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(638..838)
                     /locus_tag="OQL90_003933"
     CDS             complement(638..838)
                     /locus_tag="OQL90_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1370530.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            929..1603
                     /locus_tag="OQL90_003934"
     CDS             929..1603
                     /locus_tag="OQL90_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCS1370531.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPIGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALHPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3133..3669
                     /locus_tag="OQL90_003935"
     CDS             3133..3669
                     /locus_tag="OQL90_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HCS1370532.1"
                     /translation="MSFIQTLTGKKFNYINSTIDDIYIEDIAGALSNICRFAGHLPDF
                     YSVAQHSVLCSQLVAPEFAFEALMHDATEAYCQDIPAQLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPLLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            3660..>3861
                     /locus_tag="OQL90_003936"
     CDS             3660..>3861
                     /locus_tag="OQL90_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370533.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFD"
BASE COUNT         1174 a          839 c          806 g         1042 t
ORIGIN      
        1 tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacaacc
       61 agaagcaatc gacgccacga cgcccggagc tgcacactcg cgggcgtcat tactttctga
      121 aatgcaaaaa attttgtaga caagtgctgc atgctcctgc agcttcgaaa ttgagagata
      181 cagctcgtcg ttaattgctg tcttctcatg cggttccact acaccgtctt cgattgctga
      241 acgaatctgt tttgaataac tgccgatctg ttcaatgact tccagcagac gctggttaat
      301 atcggcgttg tccacatcct cgatgtcagg aagagacaca aagacgccat ttgcagactg
      361 cgccacagca tcagcaatga agtgagtgcc accagcacgc tgtaaaacca ttgcccatcc
      421 cagcgggaaa atctgatcgc catctgcacg aaggcggttg aataaagcgt tttctgttac
      481 atcgagccag tcagccgctt cagcgtaacc acccggcaac tttgcgatag tttttctgac
      541 agctttcacg taccactcag gctgtttttc tattttccag tgatgcttac ccacgattag
      601 cctcatcgtt ctgtggttaa aaattgaaag tgttctgcta atctttcgga tagatatccg
      661 gtcttaagtc agatttcgta attgcacctg acgtgcattg ctcaagtttt ttagccagca
      721 caaaactggc ttttttatag ccattgaaaa ccagccgtaa gtagccttgt gttgagccaa
      781 cttttccggc caactcaccc tgctgttctt tggttaaaga gtcccaatac gctttcatac
      841 aatatgtacc tccagtgtac acatcacatg attgaaatga accttcaaga tacttgtacc
      901 ttagcggtac aagggtttta atttcgttat gaaaacaatc catgacatcc ggcggtctaa
      961 cgccagaaaa ctgagagatg gtgttggcgg gaattcttcc tttgccacta tgattgatcg
     1021 cgagccaacc cagaccagca ggtttatggg agatggtgct actaaaaata tcggtgacag
     1081 catggcacga cacattgaaa aatgtttcga cctgcctatc ggatggctcg atcaagaaca
     1141 ccagacaacg aacatcacaa aaaaacctga tgtttcaatc actaacaaac aaataacgtt
     1201 agtccctgtc atatcatggg tacaggccgg agcatggaaa gaagttggct attctgaggt
     1261 tgatttgagc acagcagaaa cttatccctg ccctgtaccc tgtggcgaaa tgacttatat
     1321 cttgcgggtg attggtgatt caatgattga tgagtaccgt ccgggagaca tgatttttgt
     1381 tgatcctgaa gtccctgctt gccacggtga cgacgttatt gcgttgatgc acgatacagg
     1441 cgaaaccacc ttcaagcgat tgatagaaga tggaacacag cgttatctca aagcattaca
     1501 cccaaactgg cctgagccct acattaagat taacggtaat tgctctataa ttggcactgt
     1561 gattttctcg ggaaaaccaa gaagatacaa aataaaggcc taatcaatat ttatgaacct
     1621 gcttcggcag gtttttttat acttgacaat gtaccccatg gacacacaat gtacccccat
     1681 ggacacacaa tgtaccaaaa cgaaacaaca aacaggcagg atgcccacga agtagccgcc
     1741 tggggcatat gaagtccaag atgattcgtt agcaacaaaa cgcccatagg acgcttcgct
     1801 ctttaacaat ttgaatattt tcagcgtcaa tgatctgttt agctccctac aagtaaatgt
     1861 agcgtagcac catgataaat cctgctagtg tttagttaaa aatcgcgcga gagcaagaac
     1921 gacgtgaggg caggcaatac tgatagtgtt attgctatta gtttatcaaa aatatttaga
     1981 cgacccagat gaatattttt aatacatact ccccctaatt taacaggagt aaatgttgat
     2041 gaataacgag acatctatac tcaacaattg tatgatatga acaattatat aacataatgt
     2101 aggaaaaata aaaatggatt ggcatgacct tatatcaaag gtaattatcg gacttgttac
     2161 aggctcaatc gcagcttact taactgcgcg atatgcactc ggccgttatt ataaagaaaa
     2221 atggtgggac aaaagaatag atttgtatac gtaattagtt gactgtgttt atcttctaaa
     2281 aaacacagct gactattggt ataaacatga gttacatcac gcttcacaat tcccaaccta
     2341 ctttagttac aaatcaaaag aagaaaatga aatccttagc aaatctttct ctaatgagct
     2401 tgaaaaactc agtagaatag gtgagctatc ttcattattc ttaacagaag agtgtgggga
     2461 taaaatccgt tcattcattg aaaatgaaga aaatctattt atacaatggg tgtttgatag
     2521 tatagagttg atagatgccc acaagtcaag acttgataaa ttaaaagtat tactagacga
     2581 tattatctct gaagcaaaga gagagcttaa ggtcaataaa aaacataaca gcctaaagaa
     2641 tataaagtgg tccataacta tgccagacca aaaaaatgat gagccaatta attatcacga
     2701 caatcattaa aacactttag aacccgctgg gcgggttcta ttcaaatata ctttgcacat
     2761 cacaaataaa actataacgt ccttcttctt ttcctaatat cttatatcta cttatttata
     2821 agcggcttac agatcttcaa cagataaacc taatgaaggc gacaatgttc tgatgaatag
     2881 ccaccttttt actcaactga aagcgcgttc tgtcccttgt cattaagtgc cagttcgtta
     2941 agtccaaaac ctccggaacg cgctttcaat tgagtgggga aaataactgg cgatggcagc
     3001 cgcccgttgc actaagtgcc tctggatggg gcatttactg aaacgaaacc aaaactttta
     3061 atcgccttat ggcgagggat tcgtgcaacc aaaaatcgtg cggtgcagcg caccaattaa
     3121 ggagaactaa cgatgagttt tatccagact cttaccggta agaagttcaa ttatatcaac
     3181 tccaccattg acgatattta tatcgaggat atcgccggtg cgctttcaaa tatctgccgc
     3241 ttcgcgggac acttgccgga tttttacagc gtggcgcagc attccgttct gtgcagtcaa
     3301 cttgtggcgc cagagttcgc ctttgaagcg ttaatgcatg atgcaacaga agcgtattgt
     3361 caggacatcc ccgcgcaact gaaacgcctt cttcctgact ataaacggat ggaagaaaaa
     3421 atagacgccg taatccgtga gaaatacggg ttacccccgg ttatgagcac gcccgtgaaa
     3481 tatgccgatc tcatcatgct ggcaaccgaa cgccgcgatc ttgggcttga tgatggctct
     3541 ttctggcctc tactagaagg tatcccagca acagagatgt tcaaagttat tccactgtcg
     3601 ccaggccatg cctacgggat gtttatggaa cgttttaacg aattatcgga gttacgcaaa
     3661 tgcgcatgaa tgttttcgaa atggaagggt ttcttcgcgg gaaatgtgta ccgcgagatc
     3721 tgaaagtgaa tgaaacggat gctgaatacc tggtgcgtaa attcgatgcg cttgaagcta
     3781 aatgtgcggc actggaaaac aaagtaatac cagtgtcaac tgaactgcca ccagcaaatg
     3841 aaagtgtttt gttattcgat g
//
LOCUS       DAJVOY010000253         3772 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVOY010000253 DAJVOY010000000
VERSION     DAJVOY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.256"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..199)
                     /locus_tag="OQL90_003937"
     CDS             complement(<1..199)
                     /locus_tag="OQL90_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCS1370534.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVE"
     gene            complement(340..2373)
                     /gene="metG"
                     /locus_tag="OQL90_003938"
     CDS             complement(340..2373)
                     /gene="metG"
                     /locus_tag="OQL90_003938"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1370535.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQESRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2505..3614
                     /gene="apbC"
                     /locus_tag="OQL90_003939"
     CDS             2505..3614
                     /gene="apbC"
                     /locus_tag="OQL90_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1370536.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHCLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          906 a          966 c          969 g          931 t
ORIGIN      
        1 gctccacata ccccttcttc accttctccg caatcagctt aagctccgct tttgccgctg
       61 ccgcagcatc tgaaaaactt tttatctggc tttgcccttt ggtgccaact tttccccaac
      121 tgatatgcaa ctcgtttccc tgttgctcaa ctgcccagaa tttatgtgat ttttcgtcct
      181 gatagataaa gtgtctcatt ctgcatccct gtaattttat ccagttgaat gcagatgcta
      241 ccagtattta tgcgggttag agagagacaa aatcgcaaga aaacaacttt aggaatttat
      301 aaagaaaagg cgctgtcgat gcagcgcctt gaagggggat tatttcacct gatgacccgg
      361 tttagcaccg gcatccgggc ttagcaggaa aatatctttc ccgccaggac cggcagccat
      421 caccatgcct tcagagatac cgaagcgcat tttacgtggt gctaggttag ccaccataat
      481 ggtgtgacga ccaatcagtg cctgcggatc cgggtaagca gaacgaatac cggagaagac
      541 attgcgtttt tcaccgccga gatccagcgt caggcgcagc agtttgtcag aaccttcaac
      601 aaactctgcg ttttcaatca gcgccacgcg caggtcaact ttagcgaagt cgtcaaaggt
      661 gatggtttcc tgaatcggat catctgccag cgggccagtt accggcgcgg cagcggcttt
      721 tacttcttct ttagaggctt ccaccagtgc ttcaacctgc ctcatatcga tgcggttata
      781 cagcgccttg aacggattca ctttgtggcc cagcagcggt tgctggatac catcccaggt
      841 cagttccgta ttgaggaatg cttctgcacg ctcggtcagt ttcggcagta ccggcttcag
      901 gtaagtcatc agcacgcgga acaggttgat gcccattgag caaattgcct gcaggtcggc
      961 atcgcggctt tcctgtttcg ccaccaccca cggagcctgt tcatcgacat agcggttagc
     1021 cagatcagcc agcgccatga tttcgcgcac ggctttacca aattcacggc tttcccacgc
     1081 ttcaccaatc acttcagcgg catcagtgaa ggttttgtac aactgcgggt cagccagttc
     1141 gcttgccagc acgccgtcaa aacgcttgtt gataaagccc gcattacggg aggccaggtt
     1201 aaccactttg ttaacgatat cggcattcac acgctgaacg aaatcttcca ggttgagatc
     1261 gatatcatcg atgcgcgaag agagtttcgc agtgtagtag taacgcaggc tgtctgcgtc
     1321 aaaatgattc agccaggtgc tggctttaat aaaggtgccg cgagacttgg acatcttcgc
     1381 gccgttcacc gtcacatagc catgaacaaa caggttggtc ggcttgcgga agttgctgcc
     1441 ttccagcatg gcaggccaga acaggctgtg gaagtaaacg atatctttac cgatgaagtg
     1501 gtacagctcg gcggtggagt ctttcttcca gtattcatcg aagcttacgc tgtcgccgcg
     1561 cttgtcgcac agattcttga aagaacccat gtagccaatc ggtgcgtcca gccagacgta
     1621 gaaatatttg cccggcgcgt tcggaatttc aaaaccgaag taaggtgcgt cgcgggagat
     1681 atcccactgt tgcaggccag attcaaacca ctcctgcatt ttgtttgcca cctgctcctg
     1741 caacgcaccg ctgcgggtcc atgcctgcaa catttcgctg aaagagggca gatcaaagaa
     1801 gaagtgttca gaatcacgca ttaccggggt agcgccagaa accaccgatt tcggctcgat
     1861 cagttcagtc gggctgtagg tcgcgccgca gacttcgcag ttatcgccgt attgatccgg
     1921 ggatttacat ttcgggcagg tgcctttcac aaaacggtcc ggcaggaaca tgcctttttc
     1981 cggatcgtac agctgagaga tggtgcggtt tttaataaaa ccgttttctt tcaggcgaga
     2041 gtagataagt tctgacaact ggcggttctc ttcgctgtgt gtcgagtgat agttgtcata
     2101 gctgatgtta aagcctgcga aatcagtctg atgctcctga ctcatttcgc caatcatctg
     2161 ctccggggtg ataccaagct gctgagcttt cagcatgatc ggtgtaccgt gggcatcgtc
     2221 ggcgcagatg aagttgacct cgtggccgcg cattcgctgg taacggaccc agacatcagc
     2281 ctggatgtgc tccagcatat ggccgaggtg gattgagccg ttagcgtacg gcagtgcgca
     2341 cgtcaccaga attttcttcg cgacttgagt catagtaggc attacttctt aatagtgaaa
     2401 aggggttaga tagtaccaaa tgggaaaatg ttaagtaagt acttctctat attcttcagg
     2461 taaactatca gctgatggtt tatctataca aaaaaggagt cgggatgaac gaacaatccc
     2521 aggccaaatc accagaagcc ttgcgcgcaa tggtcgccgg gacactggcc aattttcagc
     2581 acccaaccct gaagcataac ctcaccacgc tgaaagcgtt gcaccatgtc gcctggatgg
     2641 acgacacgtt gcatgtggaa ctggttatgc cattcgtctg gcatagcgcg tttgaagagt
     2701 taaaagagca atgtagcgcc gaactgctgc gtatcaccgg ggcgaaagct atcgactgga
     2761 aactgtcgca caatatcgcc accttaaaac gcgtgaagaa tcagccgggt attaatggtg
     2821 tgaagaacat cattgcggtc agttccggta aaggcggcgt gggtaaatcc tccacggcgg
     2881 taaacctggc gctggcactg gcggcggaag gggcgaaagt cggcattctg gacgccgata
     2941 tctacggccc atcaattccc accatgctgg gcgcagaaaa tcagcgtcca acttcgccgg
     3001 acggtaccca tatggcaccg attatgtctc attgcctggc aaccaattca attggttatc
     3061 tggtcactga cgacaatgcg atggtgtggc gtggaccgat ggcgagcaag gcgttaatgc
     3121 agatgttgca ggaaaccttg tggccggatc tcgactatct ggtgcttgat atgccgccag
     3181 gcaccggtga cattcaattg acgctggcgc aaaacattcc agtaactggt gcagtcgtgg
     3241 tcaccacccc gcaggacatc gcgttgatcg atgcgaaaaa aggcattgtg atgttcgaga
     3301 aagtcgaggt tccggtgctt ggtattgtcg agaacatgag tgtgcatatt tgcagtaact
     3361 gtggtcacca tgaaccgatt ttcggtaccg gtggtgcaga gaaactggct gaaaaatacc
     3421 acacacagct gctggggcag atgccgttac acatctcctt acgtgaggat ctcgataaag
     3481 gcacgccaac ggttatcagc cgtccggaga gcgaatttac cgctatctac cgccagctgg
     3541 ctgaccgcgt tgcagctcag ctctactggc agggtgaagt cattccaggc gagatttcct
     3601 tccgcgcggt ctaacgccta ttaaccattc tggttattct tcatacaccg cagagatgtt
     3661 acattgatgc ggtgtattag tttttgccgc attaatgaac acttatttaa tactgcgaat
     3721 attctgcgga ccttcgtccg aaactctaca aagtcgattg atcatacagg ca
//
LOCUS       DAJVOY010000254         3758 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVOY010000254 DAJVOY010000000
VERSION     DAJVOY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.257"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..1411)
                     /gene="maeB"
                     /locus_tag="OQL90_003940"
     CDS             complement(<1..1411)
                     /gene="maeB"
                     /locus_tag="OQL90_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1370537.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKP"
     gene            1700..2650
                     /gene="tal"
                     /locus_tag="OQL90_003941"
     CDS             1700..2650
                     /gene="tal"
                     /locus_tag="OQL90_003941"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1370538.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            2670..>3758
                     /locus_tag="OQL90_003942"
     CDS             2670..>3758
                     /locus_tag="OQL90_003942"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1370539.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT          926 a          980 c          971 g          881 t
ORIGIN      
        1 tcggtttcgc cagtcccagc gttaccagtt cctgagtggc atgcagaacg cgcgcctctt
       61 ccccttctgg cagaacaacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca
      121 taaacaggtt ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag
      181 caatcggacg cgtcgccacg cccgactcca tcgcggcttt agcgaccgca ggagcgatct
      241 taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat
      301 cctgatcgcc atacgctgaa gccaccactt cgctctgttc cgcatgggcg agttctgcaa
      361 tcgcacgtac cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg
      421 cgccacggaa gatgaacggg aagcacagga cgttgttcac ctggttcgga tagtcagaac
      481 gaccggtgca aatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg
      541 gttccgggtt cgccagcgcc aggatcattg gcgcacgagc cattttcttc accatttcct
      601 gggtcagcac tttcgggccg gaacatccga ggaaaatatc cgcgccttca atcacatcat
      661 cgagggtacg tttgccgtcg tccaccaccg catatgcggc tttggtttcc gccatgtttg
      721 gctcacggcc ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca
      781 gacccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca
      841 ccatccgcac gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag
      901 tgctgatgat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc
      961 gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgattccgc
     1021 cgaaggtcgg ttcgagcgca gcgacaactt caataaattt gtccgggtcg agttcatcaa
     1081 cttcaatgtc aaatacatca atcccggcga atttcttaaa cagaacgccc ttgccttcca
     1141 tcaccggttt gccagccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag
     1201 agatcaccgc caccaggtta ccgcgcgcgg tatatttgta ggcttttaac gggtcttttt
     1261 cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg
     1321 ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat
     1381 caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca
     1441 aataaagtgt gtgggcaaca gcccctgccc acaacgtggc gcacattatt accccgccgg
     1501 agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat
     1561 cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt
     1621 ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac
     1681 ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt
     1741 ggcagacagc ggcgatattg agtccattcg ccattatcat ccccaggatg ccaccaccaa
     1801 tccttcgctg ttgctcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc
     1861 tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga
     1921 caaactggcg gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtc gcgtgtcaac
     1981 agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct
     2041 ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attctgatca aactggcttc
     2101 gacctgggaa ggaattagcg cggcagaaga gctggaaaaa gaaggtatta actgcaacct
     2161 gacgctgctg ttttcttttg cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat
     2221 ttcgccgttt gtcgggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta
     2281 tgtggtggaa gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca
     2341 acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc
     2401 cttaaccggc tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa
     2461 agtttcgcca gtggtacgta aattaatccc accttctcag acgttcccac gcccagctcc
     2521 catgagcgaa gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact
     2581 gtctgaaggc attcgtctgt tcgccgttga tcaacgcaaa ctggaagatc ttcttgccgc
     2641 caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc
     2701 gcgcactcag tatggatgcg gtacaaaaag ccaactctgg tcatcccggc gcgccgatgg
     2761 gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataac cctaccgacc
     2821 caaccgggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct
     2881 acagtttgct gcatctgacc ggttacgacc tgccgctgga agagctgaag aacttccgtc
     2941 agttgcattc gaaaacccca ggccacccgg agattggtta tacgcccggt gttgaaacca
     3001 ccaccggtcc acttggacaa ggtttggcga acgccgtcgg actggcgata gcagagcgta
     3061 cactggcggc gcagtttaat cagccggatc atgaaattgt cgatcacttc acctatgtgt
     3121 ttatgggcga cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcgggca
     3181 cgctaggact gggcaagctg attggttttt acgatcacaa cggtatttcg attgatggag
     3241 aaaccgaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg
     3301 tgatccatga aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc
     3361 aaagcgtgaa agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc
     3421 cgaataaagc aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc
     3481 tggcacggca aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc
     3541 acgcctggga tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg
     3601 ccgcctataa aaaggctcat ccgcaactgg cagaagagtt tacccgacgg atgagcggtg
     3661 gtttaccgaa ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc
     3721 cggcgaaaat cgctacccgt aaggcttcgc aaaatacg
//
LOCUS       DAJVOY010000255         3748 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVOY010000255 DAJVOY010000000
VERSION     DAJVOY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.258"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..345
                     /locus_tag="OQL90_003943"
     CDS             <1..345
                     /locus_tag="OQL90_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1370540.1"
                     /translation="YLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFT
                     GDIFGTAGKIARIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPF
                     SAWDKSGKLVKE"
     gene            353..1681
                     /locus_tag="OQL90_003944"
     CDS             353..1681
                     /locus_tag="OQL90_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1370541.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1728..3053)
                     /gene="rimO"
                     /locus_tag="OQL90_003945"
     CDS             complement(1728..3053)
                     /gene="rimO"
                     /locus_tag="OQL90_003945"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1370542.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3266..3649
                     /gene="bssR"
                     /locus_tag="OQL90_003946"
     CDS             3266..3649
                     /gene="bssR"
                     /locus_tag="OQL90_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1370543.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSILSLQKHAMLEKA"
BASE COUNT          973 a          836 c          926 g         1013 t
ORIGIN      
        1 tatctcaata ttgaacaact gcgtaaacaa ggggtacgga tatcgattga tgactttggc
       61 accggtttgt caaacctgaa acgtttttat gaaattaatc cagacagcat aaaggtggac
      121 tcgcaattta ccggcgatat tttcggtact gcgggaaaaa ttgcgcgcat tattttcgat
      181 ctggcacgct ataaccggat cccggtgatt gcggaaggcg tagagagcga agacgttgca
      241 cgcgaattaa ttaaattagg atgtgttcag gctcaggggt atctgtacca gaaacctatg
      301 ccattctccg cctgggataa aagtggaaaa ttagtaaaag agtagtttac gtatgtccag
      361 aatcaataag ttcgtactta cagtcagtct gctgattttt atcatgattt cagcagttgc
      421 ctgcgggatc tacactcaaa tggtaaagga acgggtgtat agcctgaaac agtccgttat
      481 tgatactgct tttgcggtgg caaatattgc tgaatatcgg cgtagcgtgg caattgatct
      541 tatcaacacg ctaaatccca cggaggaaca gctgttggtt ggtttgcgca cagcttacgc
      601 cgactcggtt tccccctctt atttgtacga tgtcggtcct tatctgattt ccagtgacga
      661 atgtattcag gtaaaggagt tcgagaaaaa ttattgtgca gatattatgc aggttgtgaa
      721 gtatcgacat gtcaaaaata cagggtttat ctcttttgac ggtaaaacct tcgtctatta
      781 cctctatccg gtaactcaca atcgtagtct gatatttttg cttggtctgg agcgtttttc
      841 tttactgtca aaatcgctgg cgatggacag cgagaacctg atgttctctc tatttaagaa
      901 cggtaaaccg gtgaccggtg atgaatataa tgctaaaaac gccatcttca ccgtttcgga
      961 agcgatggag cacttcgcct atttgccgac cggattgtac gtatttgcgt ataaaaaaga
     1021 tgtttatttg cgggtctgta cattgattat tttctttgcc gcattggtgg ctgtgatatc
     1081 gggtgccagt tgcctctatc tggtacgcag agtgattaat cgtggtattg tggagaaaga
     1141 agccatcatt aataaccatt ttgaacgcgt actggatggc gggcttttct tttcggctgc
     1201 cgatgtcaaa aaactctaca gtatgtataa ctcggcgttc ctggacgacc tgaccaaagc
     1261 aatgggcaga aaatcctttg acgaagattt aaaagcgctg ccggaaaaag gcagttattt
     1321 gtgcctgttt gacgtcgata aattcaaaaa cattaacgac accttcggtc atttgctggg
     1381 cgatgaagtg ttgatgaaag tggtgaaaat ccttaaatca cagatcccgg tagataaagg
     1441 taaaatctac cgcttcggcg gtgacgaatt tgcggtgatt tatacgggtg gaacgctgga
     1501 agagttgcta tcgattctaa aagaaatcgt tcatttccag gtgggaagca ttaatttaag
     1561 taccagtatc ggtgtagcgc attcaaatga atgtaccacc gtcgaacgct tgaaaatgct
     1621 ggcggatgag cggctgtata agagtaagaa aaacggcagg gctcagatta gctggcagta
     1681 atcattattc gcaggccgga caaatgattt tgcccggcct gaattaatta aacccggcta
     1741 ccccacaaat cgtactcatc ggcgtgctcg actttcacac gcaggatatc acccggctta
     1801 acgttggttt caccattgag ataaaccgcg ccgtcgattt ccggtgcatc tgccatgctg
     1861 cgaccaatcg cgccttcttc gtccacttcg tcgataatca ccagaatttc acggcccact
     1921 ttctcttgca ggcgctcggc ggaaatctgc tgctgcagct gcatgaaacg gttccagcgt
     1981 tcttctttaa cttcttccgg aacctggtca ggcagggcat tggcgtctgc accttcaacc
     2041 gggctgtatt taaagcagcc aacgcgatcc agacgcgctt ctttcaggaa gtcgagtagc
     2101 atctggaaat cttcttctgt ctcgccaggg aagccgacaa taaaggttga gcgtagggtc
     2161 agttccgggc agatttcgcg ccactgtttg atgcgcgcca gttggcgatc tacagaaccc
     2221 ggacgcttca tcagtttgag aatgcgcggg ctggcgtgct gcaacggaat gtccagatac
     2281 ggcaggattt tgccttctgc catcagtggg atgacgtcgt ccacatgcgg ataagggtaa
     2341 acgtagtgca gacgtgtcca gatccccagt ttcgataact gttcgcacag gctgaccatg
     2401 ctggttttta ccggctcgcc gttgtggaag ccagtacgat gtttaacatc aacgccatag
     2461 gcggaagtat cctgcgagat caccagaatc tctttaacgc ccgcatctac cagacgtttc
     2521 gcttcactta acacttcgcc aatcggacgg ctcaccaggt cgccgcgcat agacggaata
     2581 atgcagaagg tgcagcggtg attacagcct tccgaaattt tcagataggc ataatgacgc
     2641 ggcgtcagtt tcacaccttg ttctggcacc aggctcagga atgggttgtg tttcggtttt
     2701 ggcacgtagt gatgaacgtg ctccagaacc tgctcatagc tatgaggccc ggtgatttcc
     2761 agcactttcg ggtggacttc gcggatctga tcttcttttg cccccagaca accggtcaca
     2821 attaccttgc cgttttcatt caacgcttca ccaatggctt ccagtgattc ttgtaccgcg
     2881 ctgtcaataa agccgcaggt gttgacgatc accatgtccg catcgtcata gctcggtacc
     2941 acgtcataac cttcagtgcg gagttcggtg agaatacgct ctgaatcgac aaggtttttc
     3001 ggacagccaa gggaaacaaa gccgattttc ggctggggag ttactttgct catagcttaa
     3061 aaaatattca gttacaggaa aggtcagggc agggattcta cagagttctg gataaaattt
     3121 gtatcgcaat ctcattcgct ggcggaggcg aaggaaatgt aaattttgtt aattcggcgt
     3181 gaagaattga tcctggacag cattttgctc aaaaaatagc catactattt aattgcaaca
     3241 aggctggaaa gaggaggatc gaagtatgtt cgttgacaga cagcgaatcg atctgctgaa
     3301 ccggttgatc gacgcacgcg ttgacctcgc tgcatacgtg caactgagga aggcaaaagg
     3361 atacatgtcc gtcagcgaaa gcaatcatct acgagataac ttttttaaac tgaatcgcga
     3421 actgcacgat aaatcgctgc ggttgaatct tcatctggat caggaagagt ggagtgctct
     3481 tcatcatgct gaagaagcat tagcgacagc cgcagtatgt ttgatgagtg ggcaccatga
     3541 ttgcccgact gttattaccg tcaacgccga taagcttgaa aattgtctga tgagcttaac
     3601 gctgagtatc ctgagcctgc agaagcacgc catgcttgag aaggcctgaa aactaagggg
     3661 gagaaagcgt ctcccccttc atgtttaagt ttttgtaaaa atgaatttgt tatctcctcc
     3721 actgactacg ctttaagcca gagtcaat
//
LOCUS       DAJVOY010000256         3725 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVOY010000256 DAJVOY010000000
VERSION     DAJVOY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.259"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(67..855)
                     /locus_tag="OQL90_003947"
     CDS             complement(67..855)
                     /locus_tag="OQL90_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1370544.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(895..1791)
                     /locus_tag="OQL90_003948"
     CDS             complement(895..1791)
                     /locus_tag="OQL90_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1370545.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            1964..2842
                     /gene="yihU"
                     /locus_tag="OQL90_003949"
     CDS             1964..2842
                     /gene="yihU"
                     /locus_tag="OQL90_003949"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS1370546.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            2867..>3725
                     /locus_tag="OQL90_003950"
     CDS             2867..>3725
                     /locus_tag="OQL90_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1370547.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAE"
BASE COUNT          917 a          898 c          952 g          958 t
ORIGIN      
        1 aggttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt ttccgaaatt
       61 tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt cacttaacaa
      121 ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat tccgtgcatt
      181 gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta acaatgtatt
      241 ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttagatcaa attccagtag
      301 ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc ggaagccttc
      361 aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta aagtgcctcc
      421 cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac gtaggctgtt
      481 agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt ctacggttgt
      541 accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg cctcagcgat
      601 cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat tcatgacgct
      661 ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac tgagttttcg
      721 gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt cttcaatatt
      781 catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat tacctgtcac
      841 ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac cattttacgc
      901 ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc gaccgccagg
      961 ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt ttgttggcat
     1021 tttttctgcc aacgctaccg cgagtgcgcc gtgaaaaaca tcgcctgctc cagtggtatc
     1081 aaccacattg acagaaaatg cctcctgttg acacaaatga ccatcttcaa tccacaacga
     1141 tccttcagaa cccagcgtaa catacacttt acctgctgtt tgtgtcgttg cctggaacaa
     1201 accttcttca ggagactgca ggccagtcat gcgtttcagc cctggtgttg agaacacggc
     1261 gtgatcggca agcgcgacaa gtggagaaat atcctgcggt gtcatatcgg cgtctaaaag
     1321 cgttgtcaca cccgcaaggc gcgctagcga gaatgctttt tcagtaccgc tatgccagcg
     1381 aacatcggcc agaatcagat tataacgaga gaaatcgatc gcttctaacc attcggcatc
     1441 agtaccgaga tcaggacttg gataattaac gataatccgt tcgccatgct gatcaacgag
     1501 gatcgctgat tgcgacgaac gggcgttagg atatcggcgg caaaatgcgg tattgacgcc
     1561 ccaaccctca agttctgcaa gtaaggtatt tccacaactg tcatcgccga cgcgaccgat
     1621 gaaatcaacc tccacgccaa gctttgcgat tgcaacagcg gctgttgctg ccgggccgcc
     1681 accaatctca agataatgat ttgcctgata tttcccgccg ccctccggca gagtgggcaa
     1741 agaataaatt ctgtcctgaa ctgtgatacc cacgcatgca attctggtca tatttgtctt
     1801 gtcctgttta attttgtcgt ctttggtcaa tttaaatgac gcaaatgatc aaattagtga
     1861 tgcgtgtcgt gtttttgaac ataaatcaca aatatgctca aaaacagaca aaaacgaatt
     1921 ttcaattgtc attattttgt caatgtcaga caggagagta ttcatggcaa ccattgcatt
     1981 tctcgggctg gggcagatgg gtagcccaat ggctaacaat ctattgcaaa aagggcattc
     2041 attgcaggta tttgatgtca atgcacaggc cgtcgatgcg ctagttacgc agggggccac
     2101 tgcggcacaa acccaagcgg aagcagcagc aggcgctgaa tttatcatca ctatgcttcc
     2161 caatggtgac attgttcgac aggttctgtt gggggaaaaa ggcgtctgtg aggctgtttc
     2221 atctgacgcg ttattgattg atatgtcgac tattcatccc ttacagaccg atgcattaat
     2281 acgggagtta caagaaaaag gcatcagtat gatggacgct cccgtgggca gaacgtccgt
     2341 gaatgcgatt gatggcacat tgctgatttt agcgggtggc actgatgagc agattgcacg
     2401 tgcacgcccg attcttatgt gcatgggtaa tgagctggtc gaggctggcg gcccaggaat
     2461 ggggatccgc gtgaagctaa ttaacaacta tatgagcatt gcgcttaatg cgctgtcctc
     2521 cgaggccgct gtgttatgtg agtcactggg attaaatctt gatgtcgcaa ttaaggtgat
     2581 gagtggtacg gcagcaggga aaggacattt tacgaccacc tggcctggca aagtgctgaa
     2641 aggcgatctt tcacccgcct tcatggtgga tctggcgttg aaagatctgc gtattgcagt
     2701 tgacgtcgct cgggagacgg ggacaccgct caatatgggg attgcagcag aaagttatta
     2761 cgctgcagca agtcaagacg gcaaagggcg gcaggactgg agtgccctgc taaatcaggt
     2821 tcgccagcag gctggtcttt aattttatct attaacagga gtttctatgt catcccactt
     2881 caccccattt acgctgaaag atatctcccg cccaggtggt ggatttgcca tgttagctgt
     2941 cgatcagcgt gaggccatgc gcttgatgtt tgcggcagca gggcagccaa aacccatcgc
     3001 cgacagtgta ctcaccgatt ttaaagttgc cgcgacccga atcctttctc cttatgcttc
     3061 tgccgttctg gcggataaac aattttgtct tgagcaaata gtcgagcagg gcgctgttgc
     3121 taattcttgc ggcctgattg tcgctgccga tctttttatc ccaggtaatg gcatcccggt
     3181 tgatagcgtt gaaatagata tgtccgtcga cccgcataaa gcccgtgaaa tgggggctaa
     3241 ggcaatgaaa ttacttgtac tctggcgtga agatgaaccc gcagaggaac gtctggcaat
     3301 ggttgataaa tttgttcgtc gctgccgtag tgccgggctg gtgagcatta ttgaacctgt
     3361 ggtgcgtcca cctcgtcgcg gttgggactt tgatcgtgaa agcgccattg ttgccgccgc
     3421 cgcagagctt ggtggaacag aggctgatct ttacaaagcc gagatgccat tagggggcaa
     3481 aggcgatgaa aaaaccttac ttgccgcctg tcagcaactt aacgatcaga tgaagatgcc
     3541 gtgggtaatt ctttcttctg gcgttgatgc cgatattttt ggccgtgcag taagtattgc
     3601 catgaaaggt ggtgcgtcgg gcttcctggc tggacgagcg gtttgggcgt cggttgttgg
     3661 tgcacaagat ccacaaacta tgctgcgcga tgtctctgtt cctcgtttac aacggttggc
     3721 agaaa
//
LOCUS       DAJVOY010000257         3724 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVOY010000257 DAJVOY010000000
VERSION     DAJVOY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.260"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..461)
                     /locus_tag="OQL90_003951"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="OQL90_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
     gene            686..1606
                     /gene="lpxL"
                     /locus_tag="OQL90_003952"
     CDS             686..1606
                     /gene="lpxL"
                     /locus_tag="OQL90_003952"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1370548.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            1778..3002
                     /gene="mdtG"
                     /locus_tag="OQL90_003953"
                     /pseudo
     CDS             1778..3002
                     /gene="mdtG"
                     /locus_tag="OQL90_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            3085..3459
                     /locus_tag="OQL90_003954"
     CDS             3085..3459
                     /locus_tag="OQL90_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1370549.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(3460..3687)
                     /locus_tag="OQL90_003955"
     CDS             complement(3460..3687)
                     /locus_tag="OQL90_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1370550.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
BASE COUNT          911 a          946 c          952 g          915 t
ORIGIN      
        1 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
       61 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
      121 cacgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
      181 ggcgtaaacc ctctaaagcc gggtcgaagg cagagctgcg cgcgaaatgt ttcaacattg
      241 ctcgcaggta cgctgatttg cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa
      301 acattcagcg cggtaaacag ctgatataaa gcgtcacggg tcgccttagg atcggcgatg
      361 tggaaatact tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa cattttggct
      421 tttagcgcgt cgttggaaat gcggttgtgt aacactggca tggtgtacgg ttcctgcgag
      481 atgggaaagt aaaaatccgc ggcatgatat agcaattatc gataattaac atccacacat
      541 tttacgctac atttgcgcat taaaaattat ttgttattta caagcgcggc aatttcgccc
      601 agtcttcagc cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat acaattgccc
      661 gaataggttg aaaaacagga ttgatatgac gaatctaccc aagttctcca ccgcactgct
      721 tcatccgcgt tattggttaa cctggttggg tattggcgta ctttggttag tcgtgcaatt
      781 gccctacccg gttatctacc gcctcggttg tggattagga aaactggcgt tacgttttat
      841 gaaacgacgc gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga
      901 acaagaacgc cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg gcctgatgga
      961 aaccggcatg gcgtggttct ggccggaccg ccgaatcgcg cgctggacgg aagtgatcgg
     1021 catggaacac attcgtgacg tgcaggcgca aaaacgcggc atcctgttag ttggcatcca
     1081 ttttctgaca ctggagctgg gtgcgcggca gtttggtatg caggaaccgg ggattggcgt
     1141 ttaccgcccg aacgataatc cactgattga ctggctacaa acctggggcc gtttgcgctc
     1201 aaataaatcg atgctcgacc gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg
     1261 cgaagtggtc tggtacgcac cggatcatga ttacggcccg cgctcaagcg ttttcgtccc
     1321 gttatttgcc gttgagcagg ctgcgaccac gaccggaacc tggatgctgg cacggatgtc
     1381 cggcgcatgt ctggtgccct tcgttccacg ccgtaagcca gatggcaaag ggtatcaatt
     1441 gattatgctg ccgccagagt gttctccgcc actggatgat gccgaaacta ccgccgcgtg
     1501 gatgaacaaa gtggtcgaaa aatgcatcat gatggcacca gagcagtata tgtggttaca
     1561 ccgtcgcttt aaaacacgcc cggaaggcgt tccttcacgc tattaaatct cccctgccgg
     1621 atgcttcaga atggcatccg gcattaccac agcaaatccc cctgatttag cgataaaagc
     1681 tctctggatt gcgccccctg gaagtcgggc gcataattag tgtgcttatc ttttcttctt
     1741 atgttcaccg cgcctggcgc accaacagcg gattgctatg tcaccctgtg aaaatgacac
     1801 ccctataaac tggaaacgaa acctgatcgt cgcctggcta ggctgttttc ttaccggtgc
     1861 cgccttcagt ctggtaatgc ccttcttacc cctctacgtt gagcagcttg gcgttaccgg
     1921 tcactccgcc ctgaatatgg tccggtattg tcttcagcat tacattttta ttttcggcca
     1981 tcgcctcacc gttttggggt ggactcgccg accgtaaagg ccgaaaactc atgctattac
     2041 gctctgccct cggcatgggc atcgtgatgg tgttgatggg gctggcacaa aatatctggc
     2101 agtttttgat cctgcgggcg cttcttgggt tacttggcgg atttgtcccc aacgctaatg
     2161 ctcttatcgc cacacaagta ccgcgtaata aaagcggctg ggcgctgggt acgctctcca
     2221 caggcggcgt tggtggtgcg ttgctcggcc caatggctgg cggcctgctc gccgatagct
     2281 acggcttacg tccggtattc tttattacca ccagtgtgct catactctgc tttttcgtca
     2341 ccctgttttg catcagagaa aaattccagc cggtcagcaa aaaagagatg ctgcacatgc
     2401 gggaagtggt gacatcactt aaaaacccga aactggtact cagcctgttt gtcactacgt
     2461 taatcatcca ggtggcgacg ggctcaattg cccccattct gacgctgtat gtccgcgaac
     2521 tggcgggtaa cgtcagtaac gtcgccttta tcagtggcat gatcgcctcg gtgccaggcg
     2581 tggcggctct gctaagtgca ccacgactcg gcaaacttgg cgatcgaatc ggacccgaaa
     2641 agatcctgat tacagcgctg atcttttctg tactgctgtt gatcccaatg tcttacgttc
     2701 agacgccatt gcaacttggg attttacgtt ttttgctcgg tgccgccgat ggtgcactac
     2761 tccccgccgt acagacattg ttggtttaca actcgagcaa ccagatcgcc gggcgtatct
     2821 tcagctataa ccaatcgttt cgtgatattg gcaacgttac cggaccattg atgggagcag
     2881 cgatttcagc gaactacggt ttcagagcgg tatttctcgt caccgctggc gtagtgttat
     2941 tcaacgcagt ctattcatgg aacagtctac gtcgtcgtcg aataccccag gtatcgaact
     3001 gatttttcgc ctttcatact tgcaaaagcg gagaatcagc tatccttttc cctgaaacct
     3061 catcaactca aagggagaat cgtgatgacc atgtacgcaa cgcttgaaga agccattgac
     3121 gctgcacgcg aagaatttct tgcagacaac cccggcatcg acgccgaaga tgcgaatgtg
     3181 caacagttca atgcccaaaa atacgttttg caggacggcg acatcatgtg gcaagttgag
     3241 ttttttgccg acgaagggga agaaggtgaa tgtttaccta tgcttagcgg tgaagccgcg
     3301 caaagtgttt ttgatggcga ctatgatgag atagagatac gccaggagtg gcaggaagag
     3361 aatacattac atgaatggga cgagggggaa tttcagcttg agccaccgct ggataccgag
     3421 gaaggacgcg cagcagctga tgagtgggat gaacgttaat cactcatacg ggccatgatg
     3481 aatgtcgatc ggcagcagta aagtatcaaa aaccagggag aatggcagat caaggattgt
     3541 gacataacgc caggcggagt cacgcacatc ccattgcaca ccgggataat attggttgcc
     3601 atgcccctgc cccggtatag tgcgactaat aatgctgcca cagccactca gcaaggtcac
     3661 catgatgctc accacaatta aacgcatttg atacctcttc cgtaaaaaaa taccggcata
     3721 acgc
//
LOCUS       DAJVOY010000258         3719 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVOY010000258 DAJVOY010000000
VERSION     DAJVOY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.261"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..329
                     /locus_tag="OQL90_003956"
                     /pseudo
     CDS             1..329
                     /locus_tag="OQL90_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            446..595
                     /locus_tag="OQL90_003957"
     CDS             446..595
                     /locus_tag="OQL90_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001500869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370551.1"
                     /translation="MSHYKVQQMLSCQKRRQKVQPVMQSGMQRPQQKKPGSQQKAHSQ
                     RNKAG"
     gene            628..1291
                     /locus_tag="OQL90_003958"
                     /pseudo
     CDS             628..1291
                     /locus_tag="OQL90_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117976.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            1377..1889
                     /locus_tag="OQL90_003959"
     CDS             1377..1889
                     /locus_tag="OQL90_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370552.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            1965..2177
                     /locus_tag="OQL90_003960"
     CDS             1965..2177
                     /locus_tag="OQL90_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1370553.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(2319..2644)
                     /locus_tag="OQL90_003961"
                     /pseudo
     CDS             complement(2319..2644)
                     /locus_tag="OQL90_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(2780..3028)
                     /locus_tag="OQL90_003962"
     CDS             complement(2780..3028)
                     /locus_tag="OQL90_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1370554.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(3383..>3719)
                     /locus_tag="OQL90_003963"
     CDS             complement(3383..>3719)
                     /locus_tag="OQL90_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1370555.1"
                     /translation="VLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGI
                     DTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVR
                     SVEEILHAL"
BASE COUNT         1005 a          795 c         1031 g          888 t
ORIGIN      
        1 tcagaaaatc cggatgaagc cgggcgttac agtatggacg ttgagtatgg tcagtacagc
       61 gttattctgt tggtggaggg attcccgccg tcacatgccg ggactatcac cgtgtatgaa
      121 gattctcaac ccggtacgct gaatgatttt ctcggtgcca tgactgagga tgatgcccgt
      181 ccggaggcac tgcgtcgctt tgaactgatg gtggaagagg tggtgcgtaa cgcagaggag
      241 gcgaagaaga atgccggaga ggcggagacg tcagcgagga atgccggcat atcagccagt
      301 caggcagaag agagcgctgc aaatgctgac acttcagcag gggatgcatc ggagtcagcc
      361 cggcaggcgg cagaaagtgc agcctcagca aagcagtcag aggaggcgtc ctcgtcctcg
      421 gcctctgcgg ccgctcaaaa agccagtgag tcattacaaa gtgcaacaga tgctgagttg
      481 tcaaaaaaga cggcagaaag tgcagccggt aatgcagtca gggatgcaac gaccgcaaca
      541 gaaaaagccc gggagtcagc agaaagcgca cagtcagcgg aacaaagcag gatagcggcg
      601 gaagaggccg taaaccgaat ccccaccgtg gtgggacctc ccgggccaaa gggggaaccg
      661 gggcccgcgg gtcctcaggg gccgaaggga gataaaggag agcgtggcga caccggcccg
      721 gcaggggcca aaaggtgaca aaggcgatcc gggagtggct ggaccagcag gtccggcagg
      781 tgcgccgggg ccgaaaggcg ataaaggtga tccgggagta gcaggtccag caggtccggc
      841 ggggccacag ggaccgaagg gagacacagg agcccccggg caaggaacag aactgcttac
      901 tactgccaat acatggactc aggcacaaac ttttaatggt ggtattaatg gcaatttgac
      961 ggtgaccgga aacggctcat ttaacgatat tcagatccgt tcggataaac gcaacaagcg
     1021 aaatctggta aaactggata atgcgttaga tcgtctggag gcacttactg gttatcttta
     1081 cgagatacag tactctgccg acggttggca aacgtcggtt ggtttaattg ctcaggatgc
     1141 acaaaaagca ttgcctgaac tggtaactga agacgcagac gttatatctg gtgaaaaacg
     1201 tctgcgtctt aactacaacg gcataattgc attgttagtc gagggcttta aaacacttcg
     1261 tcatgagatt aaagaactcc gggagaagta aacgacagct gttgtagttt ctggtttcta
     1321 ctgaatttaa attgtggggt gacactcacc ccacgcattc agaaggggga gatgagatgg
     1381 gggtaacatc gggatgggta ggctcttcgg ctaagagcga aacaggtgag cagtggatgg
     1441 gagccgccgg aaccaaactg ggactgagta aaccttttat gatgagccaa atggttgggc
     1501 gaactatggg ctgtaaaatt gcaactgagt actataaatg gaaatcctct gacaaggttg
     1561 ataactgggg cgcagttggc gctgactggc ctttagaaga aaaaagtaaa ggtacaatta
     1621 caaacgccgc aagctgcgga tcagggaggc tggtaggggc tgtcgttaca ctttctcact
     1681 ttttgacgaa ctctacaccg acagctgctg tttatttagc cggtggtaaa gcaggtaaca
     1741 tcaccgtaaa cgtaggtggt gctacacaaa ccatgattta tcagggcgtt gttagtgggt
     1801 tccagtatta ctggtcaggt tctgttagtt ccgctttcgt ggaggcaatg aaaaagacgg
     1861 gagtacccca ggatctaaaa attagttaaa tggtaaataa ataattttaa aaacttcacg
     1921 ttctacatac cggagacgcc ggatatatag gatattgttt taagttgcca gagaaatttt
     1981 tccggacgga tgctgataat aatgatgtgc caatgacggc agcatcatgg atggcactga
     2041 gtgaagcgac agagcaggcg atgtttgcga agggagtgga gattaataca cggcagttgc
     2101 agatgaaagc cgaggttgag gcgctgacag acctgaaggc gatccgcagt tatgttgtcg
     2161 gatggcctgc tggctgaata aaaaaacggg accacgacca gtcccggaac catgagtttt
     2221 agggtattag tttgttatca tagttagcgt gctaagtatg ccatgtcagg ttgattagtg
     2281 aagtgatgtt gttcgcattt ttgcacggcg gagaatattc agatttttgg aaatccatat
     2341 ttttcccgtg cgcgattaca tgcttcattt acgatgctat tttcgccgga cattgcgaat
     2401 tccctgcatg tggacggttt ttgtaaacag agcaatatgc gttttctccg gggggggccg
     2461 gcaagagcgc cacatcgggg atttttctga ttggtgccgc gcatacagcg atgaaaagga
     2521 gatatttgct cagtgagact gaccgaaacc tttccgccag catcgtcagc ttctgcccag
     2581 taaaaagaga cgcggaaaat gcacaacagg caccacacgt catgcatgga ttcagattgc
     2641 tcataattca ccagtacagc tataaatcgt aaagagaaca gcggtacatc gtatgtaaga
     2701 acgtaccgcg gctggctggt gaacttccga tagtgcgagt attgaatgat ttccagccgt
     2761 taccgatttt acgtgctaat tagtgaacaa accactcgtc agcagactcc caggtatctt
     2821 tcagagtttc ctgaacaaaa gttttagctg aatctttatc ggcggtgcgc gtaacagaaa
     2881 ggccatcgtt gctggtagct tttacgatca cctctacatc gtcataacgt ttactgatgc
     2941 gtcgggttaa ttcttccttt aacgcatcca cagcaccggt tggcatttta gtcatttttt
     3001 ctttggctat gcagatttca atacgcataa aagtccctct acactgtgtt tgtatacagt
     3061 atcattttta actgtatgga taaacagtat taagggtgaa ggaaagtgat tttttggggc
     3121 atgcatgggg caaaaatatt tgttgtgggg cattttgggg catgattggg acactttgtt
     3181 acatatgaac tttgccgatt tttatatgac atggaaatac aacgctttga ttcttctaaa
     3241 taaaaaacat gctcttgggc gttctttagt gatttttaaa aattccgtat cacgcagtta
     3301 aagtggcggg catactcttc aaggctggtg atccccaacc gcacccattt aggatgcgac
     3361 cattgcggta gaccgatgta aatcataacg cgtgaaggat ctcttccacg ctacgcacac
     3421 gggcgatgcg cgggtagata tgattaatgc tgttattgtg ttgctcggcg ctggcggcac
     3481 tacaggcgtc ttcggcaatc accagattaa agccaagttc ccaggcattg cgggcggtgg
     3541 attcaacacc gatattggtc gagatcccac ataacactat tgtatcgata cctcggcggc
     3601 gcagttgcaa ctccagatcc gtaccgtaaa acgcacccca ctgacgtttg atgatttcga
     3661 tatcactgtc agttgcacct aatgcagctg gatgttgcca ccagttttcg ggcaacact
//
LOCUS       DAJVOY010000259         3681 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVOY010000259 DAJVOY010000000
VERSION     DAJVOY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.262"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(123..2192)
                     /gene="glyS"
                     /locus_tag="OQL90_003964"
     CDS             complement(123..2192)
                     /gene="glyS"
                     /locus_tag="OQL90_003964"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1370556.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2202..3113)
                     /gene="glyQ"
                     /locus_tag="OQL90_003965"
     CDS             complement(2202..3113)
                     /gene="glyQ"
                     /locus_tag="OQL90_003965"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1370557.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3208..3507)
                     /locus_tag="OQL90_003966"
     CDS             complement(3208..3507)
                     /locus_tag="OQL90_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1370558.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
BASE COUNT          851 a         1001 c          947 g          882 t
ORIGIN      
        1 aatctcataa aattaatata tgagataatc tttattcagc agaagattat taaaggttgc
       61 tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta ataacggcgt
      121 tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag tttctccagc
      181 atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac tttatcgaag
      241 aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg gtaacgacct
      301 tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc cagtttaatt
      361 tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc gtcagatttc
      421 gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc atccagggta
      481 cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt cggacgacgc
      541 gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca ggcgcggaag
      601 cgaccgagca taaagtcgat aacatcatca accacgttgg cgttagtcag cttatcgcca
      661 tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt cttctcaacg
      721 ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt gtcgcctttc
      781 ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc aatcgccagc
      841 gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg atactgctca
      901 ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg catccccata
      961 acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca cttagacagc
     1021 aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat ccagccagcc
     1081 agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg gaacagcacg
     1141 gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt gaagaagaac
     1201 tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat ttgctgcgga
     1261 tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc atcgttcgca
     1321 tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc agccggcacc
     1381 gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac cagcgaagcc
     1441 acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg cgctgcttct
     1501 tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac tttcccgcgc
     1561 tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg ttcgcccata
     1621 aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatagttgc cggaatgact
     1681 ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac gtcgcttgcg
     1741 ccccaacgca tcagtttcgg gatcggcagt ttcgccagag aagtcgcaac catattcggc
     1801 agcagtgctt cggtgctttc gcccttcaca tgggcgcgat acagcagcca ttcgccttta
     1861 tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc ccaaccttct
     1921 gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg gccgcgtttt
     1981 tcgatttcac gatccggttg cgcttccgcc aggttagcta ctttcagcgc cagacgacgc
     2041 ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag ctccacagta
     2101 aaattcgcag caaaggactc aaccaggctg cgcagtgctt ttggtggcag ctcttcagtg
     2161 ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc tttgttgcac
     2221 atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc tttggtcagg
     2281 gtgcgaatgc gcagaatgta gcgctgacgc tcggtgacgg agatggcttt acgcgcatcc
     2341 agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag cggcagcgga
     2401 ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcaaagca ggtgaacagg
     2461 aagtccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt ctgatggaac
     2521 acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc gtaaacgctg
     2581 tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc gccggtaacc
     2641 ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac ttccatgccg
     2701 tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc ccagttatct
     2761 tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag agaaccgagg
     2821 tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg atagtagtgc
     2881 tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga aggctgaaca
     2941 taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg gtgagaggtt
     3001 cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg agcccagtaa
     3061 tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg catattattt
     3121 cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga tggaagatat
     3181 acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa aatggccatc
     3241 tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca tttcatagct
     3301 tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt tataacgttt
     3361 tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat caacctttac
     3421 ccgttgtgct ttctgcacgg gtgacggtgt agaacaaccc actagcacca taagcgccat
     3481 tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg tgagtcttat
     3541 tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct ataagctgta
     3601 gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc agactttttt
     3661 taaccagata cagagacaca g
//
LOCUS       DAJVOY010000260         3671 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVOY010000260 DAJVOY010000000
VERSION     DAJVOY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.263"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            243..605
                     /gene="queD"
                     /locus_tag="OQL90_003967"
     CDS             243..605
                     /gene="queD"
                     /locus_tag="OQL90_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1370559.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            683..1951
                     /locus_tag="OQL90_003968"
     CDS             683..1951
                     /locus_tag="OQL90_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1370560.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1942..2202
                     /locus_tag="OQL90_003969"
     CDS             1942..2202
                     /locus_tag="OQL90_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1370561.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFSITYRFG"
     gene            2219..2794
                     /locus_tag="OQL90_003970"
     CDS             2219..2794
                     /locus_tag="OQL90_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCS1370562.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2942..>3671)
                     /locus_tag="OQL90_003971"
     CDS             complement(2942..>3671)
                     /locus_tag="OQL90_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1370563.1"
                     /translation="LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVT
                     SLDIPTVSVRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGA
                     LPDWLVSTEDLKNVTRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVM
                     NGGVDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQA
                     DVGVVDDWKVVLEALVTNIHADCQ"
BASE COUNT          859 a          947 c          950 g          915 t
ORIGIN      
        1 aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc aaatcgacta
       61 acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacccaat gaaaaacaaa
      121 tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc gttttagata
      181 atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt agagaaatta
      241 tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc ttaccacacg
      301 tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg cgactggaaa
      361 ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa ctaaaagcgg
      421 cgtttaagcc aacttatgag cgcctcgatc accattatct caatgatatt ccaggtctgg
      481 aaaacccaac tagcgaagtt ttagcaaaat ggatttggga tcaggttaaa cccgttgtgc
      541 cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc tggttgtatc tatcgcggcg
      601 aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta gggtattgtg
      661 ttcaggatat ctggggcatg acatggaaga cgactgcgac attattatta ttggtgccgg
      721 tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg ttttgttact
      781 ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc ggacggttat atacccatgc
      841 actcgcggaa ctcctccctc agtttcatct gaccgcgcct cttgaacgac gcatcactca
      901 cgaaagcctt tccctgttaa cgcccgatgg cgtaacgacg ttttccagct tacagcccgg
      961 cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg ccgaagccga
     1021 aaaagaaggt gtcgaatgta tccccggagc gacggtggat gcactgtatg aagaaaacgg
     1081 cagagtctgt ggcgttattt gtggtgacga tattctccgc gcccgttatg tggtgctggc
     1141 agaaggtgcc aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc ctgctggcga
     1201 agcgatggcg ttggggatca aagaagtgct gtcgctggaa acatccgcta ttgaagaacg
     1261 ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggaa tctgtgatga
     1321 cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag ggattgtttg
     1381 cccgctctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctga ctcgctttaa
     1441 agcgcatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt atggtgcgca
     1501 tctggtgcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta acggctggca
     1561 gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc gcggcatggg
     1621 tatggcgctg actggcgcgc aggcggcaca aacgctgata agcgcctgcc agcaccgcga
     1681 gccgcaaaat ctgtttgcgc tttatcatca caacgtagag cgcagcctgc tatgggatgt
     1741 tctacaacgt tatcagcatg ttccggcgct tttgcaacgc cctggctggt atcgggcgtg
     1801 gcctgcgtta atgcaggata tttcccgcga tttatgggat cagggtgata aacctgttcc
     1861 accgctgcgc cagttattct ggcgtcattt acgtcgtcat ggcctgtggc atctggcggg
     1921 cgatgttatc aggagtctgc gatgtctgta gcccataatc tctggcgcgt tgctgatgcg
     1981 ccgcacattg ttccggctga ctccgttgag cgtcagatgg tacaatggtt gattaacgcc
     2041 tgtccggcag gtcttttttc gctcacaccg gaaggtgact tacgcgttga ttatcacggc
     2101 tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat ccacactaca acagtggcgc
     2161 tatccgcctt ccggattcag catcacctac cgctttggat aaataataag gataatttat
     2221 gcccctctta cacctgctcc gccagaatcc ggtgattgct gccgttaaag ataatgccag
     2281 cctgcaactg gcaatcgact ctgaatgcca gtttatttcc gtgttgtacg gcaatatctg
     2341 caccatcagt aatatcgtta aaaagattaa gaacgccggg aaatatgctt ttattcatgt
     2401 tgatttgctg gaaggcgcgt caaataaaga agtcgtgatt cagtttttga aactggtgac
     2461 cgaggcggac ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa gagcggaagg
     2521 ttttttctgt attcatcgcc tgtttattgt tgattcaatt tcgtttcaca acattgataa
     2581 gcaagttgcg caatcgaatc cggattgtat tgagatcctg ccaggctgta tgcccaaagt
     2641 gctgggctgg gtgacagaga aaatccgcca accgctgatt gccggtgggc tggtgtgcga
     2701 tgaagaagat gcgtgtaatg cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac
     2761 cggggtctgg acgttagcga aaaaattact ttgacgggat aatcgtaacc aattgaattt
     2821 ggtttgattt gtaggccgca cgccacatcc gacattcagc gcctgatgcg acgcttgacg
     2881 cgtcttatca ggcctacaag tccccaggcc agaaccgtag gcaggatcat ccggcaacac
     2941 attattggca atctgcgtga atgtttgtca ccagtgcctc cagcaccact ttccagtcat
     3001 caaccacgcc aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga ttaatcgcca
     3061 ccacaaactt gctattgcgt acgcccgcca ttaacgctgc tgcgccggaa gcgccaacaa
     3121 caatacacac ctcaggtgcc agcaaatgtc cggaaatacc aatcactttt tcagcatcaa
     3181 cgccaccgtt catcactcgc gcccgactgt agcccacttc ggctcccagc ttttctgcca
     3241 gcatagcgat ttcctggtta tctgcctctc cgccctgccc caccaccagc acccgtctgg
     3301 cctcagctaa tgggtcgcga gtaacatttt tcagatcctc tgtgctaacc agccagtcgg
     3361 gaagcgcacc cgggacaatg ttcagttgtt gtattccaga gggcaacgtg gcgtttttcg
     3421 ctgctcccgc ctgacgcgcc agagaaaggc atagcgggcg tttttctgtt tgcagcgtcg
     3481 ccgttagcgc atttccccag tgggatttgc gcacgcttac ggttggaata tctaacgaag
     3541 tcacctggca gatgcttgca ccatgtaaac gccaggccag ccgggtcgac agttcatcgc
     3601 caaacgttcc cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac tgttccacca
     3661 acgcatccag c
//
LOCUS       DAJVOY010000261         3587 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVOY010000261 DAJVOY010000000
VERSION     DAJVOY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.264"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(623..2332)
                     /locus_tag="OQL90_003972"
     CDS             complement(623..2332)
                     /locus_tag="OQL90_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCS1370564.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="OQL90_003973"
     CDS             complement(2764..3483)
                     /locus_tag="OQL90_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCS1370565.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1066 a          720 c          639 g         1162 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcaccct ctggatgtga cctttatcca
      421 tgaagaagat ttggccatac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtag
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaacatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgaga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac ggtcaggaat caggctgcat acgctgtgcc
     2701 aaatctaatc gtagttattc taaccagttt tccagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttc
//
LOCUS       DAJVOY010000262         3555 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVOY010000262 DAJVOY010000000
VERSION     DAJVOY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.265"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..285
                     /locus_tag="OQL90_003974"
                     /pseudo
     CDS             1..285
                     /locus_tag="OQL90_003974"
                     /inference="COORDINATES: protein motif:HMM:NF019996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(381..1118)
                     /locus_tag="OQL90_003975"
     CDS             complement(381..1118)
                     /locus_tag="OQL90_003975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370566.1"
                     /translation="MALPFAADACLVASSFEADADEADDDAELADDAAAVFEDSALVS
                     DAFAFVSDVFAAEADLAAALACSVASPAFVVAVEADDAALSADFPAAVALAEACPALV
                     DAALADDAAAVFEPAAAEALCPAAVSEDLAFVSDVFAAFAEFPAAVAEEAALLALEDA
                     FVSDDFAASFEAAASRAEVAASDAFVVAVDAEVAADCCDAAAFVSDVFAALALVAAAL
                     FEDSAAAALFDASVAFVDAVPALEVAD"
     gene            1076..1288
                     /locus_tag="OQL90_003976"
     CDS             1076..1288
                     /locus_tag="OQL90_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370567.1"
                     /translation="MRRPDRRQQRRVAPRRHPRRRQRQLAVRRRQLRAKVRRNLQQRA
                     PRQRQNGQRILHPPWRLRMRARRKRG"
     gene            1237..1389
                     /locus_tag="OQL90_003977"
                     /pseudo
     CDS             1237..1389
                     /locus_tag="OQL90_003977"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1366..1545
                     /locus_tag="OQL90_003978"
                     /pseudo
     CDS             1366..1545
                     /locus_tag="OQL90_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2224..2896
                     /locus_tag="OQL90_003979"
                     /pseudo
     CDS             2224..2896
                     /locus_tag="OQL90_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            3069..3491
                     /locus_tag="OQL90_003980"
     CDS             3069..3491
                     /locus_tag="OQL90_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1370568.1"
                     /translation="MAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVT
                     WLSPEGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIA
                     TEEETSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
BASE COUNT          952 a          929 c          987 g          687 t
ORIGIN      
        1 tctgaaaatc cggatgaagc cgggcgttac agcatggacg ttgagtacgg tcagtacagc
       61 gttattctgt tggtggaagg attcccgccg tcacatgccg ggaccatcac cgtgtatgaa
      121 gattcccgac ccggtacgct gaatgatttt ctcggtgcca tgacggagga tgatgcccgt
      181 ccggaggcac tgcgccgttt tgagctgatg gtggaagagg tggcgcgtaa cgcgtccgtg
      241 gtggcacaga acacggcagc cgcgaagaag tcagccagtg atgccagcac atcagcccgt
      301 gaggcggcaa cccatgcgac tgatgctgca ggctcagcac gcgcagccag cacgtcagcc
      361 ggacaggccg cgtcgtcggc tcagtcagcg acttccagcg caggaacggc atcaacaaag
      421 gccactgaag catcaaaaag tgctgccgct gcagagtcct caaaaagcgc ggcagctacc
      481 agtgccagtg ccgcgaaaac gtcagaaacg aatgctgcag cgtcacaaca atcagcagcc
      541 acttctgcat ccaccgcgac tacgaaagcg tcagaagctg ccacctcagc ccgggatgcg
      601 gcggcttcaa aagaggcggc aaaatcatca gaaacgaacg catcctcgag cgccagtagc
      661 gcagcttcct cggcaacagc ggcaggaaat tccgcgaagg cggcaaaaac gtcagagacg
      721 aacgccaggt cttctgaaac ggcagcggga cagagcgcct cggctgcggc aggctcaaaa
      781 acagcggctg cgtcgtctgc cagtgcagcg tcaacaagtg ccgggcaggc ctcagccagt
      841 gccaccgccg ccggaaaatc ggcagaaagc gccgcatcgt ctgcttcaac agccacaacg
      901 aaggctggcg aagccactga acaggccagc gcagcagcga ggtctgcttc cgcagcgaag
      961 acatccgaaa cgaacgcgaa agcgtcggaa accagcgcag aatcctcaaa aacggctgcc
     1021 gcatcgtcag ccagttcggc gtcgtcatcg gcctcatcgg cgtctgcttc aaaagatgag
     1081 gcgaccagac aggcgtcagc agcgaagggt agcgccacga cggcatccac gaaggcgaca
     1141 gaggcagctg gcagtgcgac ggcggcagct cagagcaaaa gtacggcgga atctgcagca
     1201 acgcgcgccg agacagcggc aaaacgggca gaggatattg catccgccgt ggcgcttgag
     1261 gatgcgagca cgacgaaaaa ggggatagta cagctcagca gtgcgaccaa cagcacttcc
     1321 gagtcactgg cggcaacgcc aaaagccgtt aaggccgcgt atgacctggc taacgggaaa
     1381 tacaccgcac aggatgcaac gacagcacag aaagggataa tccagctaag cagcgcgacc
     1441 aacagcacgt ctgaaacgct ggcggcaacg ccaaaggcag taaaagcagc caatgacaat
     1501 gctgagaaac gtctgcagaa agatcagaac ggtgcggata tccctggcaa agacaccttt
     1561 acgaaaaata ttggtgcctg ccgtgccttc ggtgggtcag taagcacaac aacaggaaac
     1621 tggacgactg cacagtttat cgagtggctg gattctcagg gagcatttaa ccatccatac
     1681 tggatgtgca agggttcctg gtcttatggc aataataaaa tcattactga tactggctgc
     1741 ggtaatattc atctcgccgg agctgtcatt gaagtaatgg ggataaagtc agcgatgacg
     1801 atccacatta ccacaccgac cacctccact ggtggtggaa caactaacgc ccagtttacc
     1861 tatattaatc acggaacaga ttattcacct ggctggcgaa gggactataa ctccagaaat
     1921 aagccaacgg catcagagat cggggcgtta ccgtcaggtg gaacagcagt atcatcagtt
     1981 aatctgtctt caaaaggtcg ggtaaccgcg ctgacagaca atacgcaggg ggcaacaggt
     2041 cttgagttat acgaggtgta taacaacgga tatccaacag cgtatggaaa tatcattcac
     2101 ctgaaaggga tgacagccgt tggcgaaggt gagttactca tcggctggag tggtacaagc
     2161 ggtgctcatg ctccggcatt tattcgttca cgacgggata cgaccgacgc aaactggtcg
     2221 ccgtgggcgc agctttacac ctcggctcat cctcctgcag agttttatcc agtcggtgca
     2281 ccaatcccgt ggccatcaga taccgttccg tctggttatg ccctgatgca ggggcagact
     2341 tttgacaaat ctgcttaccc gaaacttgca gccgcttatc cgtcaggcgt gatccctgat
     2401 atgcgtggct ggacgattaa gggcaagccc gccagtgatc gagccgtatt gtctcaggaa
     2461 caggacggca ttaaatcaca cacccacagc gccagcgtat ccagtacgga tttggggacg
     2521 aaaaccacat cgtcgtttga ttacggcact aaatccacga ataacactgg tgcgcatacc
     2581 catagtgtta gcggtacggc tgcttcagcc ggtgcacata cccattcgat gacatttgtt
     2641 tcaggtggtt ccagtggtgc tccgggaagt ggatcacctg attattctaa atacagtgtt
     2701 aacacttctt ctgcaggcgc tcatacgcac tctgtatcgg gtactgctgc aagcgcaggt
     2761 gcacacgcac atactgtcgg tattggtgct catacgcact ccgttgcgat tggttcacat
     2821 ggacatacca tcaccgttaa cgctgctggt aacgcggaaa acaccgtcaa aaacatcgca
     2881 tttaactata ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca
     2941 taaaaattta taatctgctg gccggaacta atgaatttat tggtgaaggt gatgcatata
     3001 ttccgcctca tacaggtctg ccagcaaaca gtactgatat tgcaccgcca gatattccgg
     3061 caggcttcgt ggctgttttc aacagtgatg aggcatcgtg gcatctcgtt gaagaccatc
     3121 ggggtaaaac ggtttatgac gtagcgtcag gggacgcgtt atttatttct gaactcggtc
     3181 cgttaccgga aaatgttacc tggttatcgc cggaagggga gtttcagaag tggaacggca
     3241 cagcctgggt gaaagatgca gaagcagaaa aactgttccg gatccgggag gcggaagaaa
     3301 caaaaaacag cctgatgcag gtagccagtg agcatattgc gccacttcag gatgctgtag
     3361 atctggaaat cgcaacggag gaagaaacct cattgctgga agcctggaaa aaatatcggg
     3421 tgttgctgaa ccgtgttgat acatcaactg cacctgatat tgagtggcct acgaaccctg
     3481 tcagggagta atcattggga ttatgccgca gcacgtctta agcaagaacg tgctgcggtt
     3541 ggatgctatt ttttc
//
LOCUS       DAJVOY010000263         3541 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVOY010000263 DAJVOY010000000
VERSION     DAJVOY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.266"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..157
                     /locus_tag="OQL90_003981"
                     /pseudo
     CDS             1..157
                     /locus_tag="OQL90_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
     gene            139..1521
                     /gene="torZ"
                     /locus_tag="OQL90_003982"
                     /pseudo
     CDS             139..1521
                     /gene="torZ"
                     /locus_tag="OQL90_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(1686..2657)
                     /gene="cmoB"
                     /locus_tag="OQL90_003983"
     CDS             complement(1686..2657)
                     /gene="cmoB"
                     /locus_tag="OQL90_003983"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1370569.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2654..3397)
                     /gene="cmoA"
                     /locus_tag="OQL90_003984"
     CDS             complement(2654..3397)
                     /gene="cmoA"
                     /locus_tag="OQL90_003984"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1370570.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
BASE COUNT          924 a          875 c          949 g          793 t
ORIGIN      
        1 tggcaaacag ccgatcgttc tgttgcaggg ttatcaaatg cagggcagtg aaaacacgct
       61 ctacctggcg gcaggtcaac ggctggcgct agccacatta agtgaagaag gtatcaaggc
      121 gctcacgcta aacggggaat ggcaggctgg ggaattcagc gccagcaata tggtgaacag
      181 aaacactgga tgctggtgac gctggcggcg gtgttagggc agattggtac accgggcggc
      241 ggttttggtt tctcttatca ttactccaat ggtggcaacc cgacacgtgt tggcggcgtg
      301 ctgccggaaa tgtctgcggc gattgccgga caggccagcg aggccgctga tgatggcgga
      361 atgagggcga ttcctgttgc ccgtatcgtc gatgcgctgg aaaatcctgg cggcaaatat
      421 cagcataacg gtaaagagca aacgtatccg aatatcaaaa tgatctggtg ggcgggtggc
      481 ggtaacttta cccatcacca ggatactaat cgtctgatta aagcatggca gaaaccggag
      541 atgatcgtcg tttctgaatg ctactggacc gcagctgcta aacatgcaga tatcgtatta
      601 ccgatcacca catcgtttga gcgcaatgac ttgacgatga ccggtgatta cagcaaccag
      661 catattgtgc cgatgaagca ggctgtcgct ccgcaatttg aagcgcgaaa cgattttgat
      721 gtgtttgccg atcttgcgga attactgaaa cctggcggaa aagagatcta taccgaaggt
      781 aaagatgaaa tggcgtggct gaaatttttc tatgatgccg ctcagaaagg tgcccgtgcg
      841 caacgcgtga ctatgccgat gtttaatgcc ttctggcagc aaaataaact gatcgaaatg
      901 cgccgcagcg agaagaacga acagtacgtt cgttatgctg atttccgcgc cgatccggtg
      961 aaaaatgcgc tgggtacgcc aagcggcaaa attgagattt actccaaaac gctggaaaaa
     1021 tttggctata aggattgccc ggcacatcca acctggcttg cgcctgatga gtggaagggt
     1081 accgccgacg agaagcagtt gcagcttctg accgcacatc cggcacaccg tttacatagt
     1141 cagcttaact atgcggaact gcgtaaaaaa tatgcggttg cagatcgtga accaatcact
     1201 attcataccg aagatgctgc tcgctttggt attgcgaatg gtgatctggt gcgtgtgtgg
     1261 aacaaacgtg gtcagattct gacaggcgcg gtggtgactg acgggatcaa aaaaggcgtg
     1321 gtatgcgtgc atgaaggtgc atggccagat ctggaaaatg gcttgtgtaa aaacggcagt
     1381 gcgaacgtgt taacggcgga tatccccagc tcgcagctgg caaatgcctg tgccggtaac
     1441 tctgcgctgg tgtatatcga aaaatatacg ggtaacgcac cgaagttaac ggcatttgat
     1501 aagcctgctg ttcaggcgta agaagtgaat agcgcccgga aacgggcgct gaggttatgc
     1561 aaacgtaaaa ctgcctgatg cgctatgctt atcaggccta catagtccct gcaatagatt
     1621 gaattagcaa gggtttgtag gccggataag gcattcacgc tgcatccggc agtaatacca
     1681 gacctttacg gcttgcgcgc aatcagcacc gcgcgtttag gcgcaggata accttcaact
     1741 gtcttactcg gatcatgtgg gtcgaggaaa tcggacagag attcggtaac catccattca
     1801 gtgcgtcgct gctcttcagt agtggtaacg cacacatcca caacgcggat atctacaaaa
     1861 ccacacttct tcagccagtt tttcagcgcc agcgcggaag ggatgaaata gacattacgc
     1921 atttgcgcgt aacgatcgcc tggtaccagc accgtgttct catcgccatc aataaccagc
     1981 gtttccagca ccagttcacc ctcattcacc agttgatctt tcaactgcca gagatgctcc
     2041 agcggcgaac ggcgatggta aagcacgccc atcgaaaaga cggtatcaaa ggctttcagt
     2101 gccggaagct gttcaatacc taacggtaac aaatgtgcgc gctgatcgtt acccagtagt
     2161 ttacgcaccg cttcaaactg gcacagaaac agttgcgtag ggtcgatccc caccgcgagg
     2221 tgcgcgcctg cgccaatcat gcgccacatg tgataaccgc tgccgcagcc gacatccaga
     2281 atggtgcgtc cggttaaatc agaaagatgg ggcagaacac gatcccattt ccagtcggaa
     2341 cgccattcgg tatcgatgtt gacgccatac agtgagaacg gccctttgcg ccatggcatc
     2401 aggttccgca taagcgtttc aatgcgttta atttgcccgg tgctcagtgg ctcttcgctt
     2461 tcggcggtta cgctatgcaa taaatccaga cgatacggtt taatttcagg cagaaattcc
     2521 accgcgttgg accactgctt aaacagcccg tgctgctgct cgcgctgcca gttagcaatc
     2581 tgcgcgggca gcgtttcgag ccagtgtgaa agatgatttt tggcaatcag agaataaaag
     2641 ttaccaaagt cgatcatgca gcgtcctctg cttttaatgc caccagtgaa ccaaagttaa
     2701 agcactggaa ccacagctcg ctatgctcaa aaccggcttt atgcaggcgt gctttatggg
     2761 tttccacgga atcggtcagc atcacgtttt ccagcatgct gcgcttctgg ctgatctcca
     2821 gttcgctgta accgttggca cgtttaaagt cgtggtgcat gttgaacaac agttcaccaa
     2881 ctttggcatc ttcgaaactg aatttttccg aaagcaccag cgcgccgccc gggttcagtc
     2941 cttgataaat tttatccagt aatgcctggc gctcggaagg ttccaggaat tgcagggtaa
     3001 aattcagcac caccatcgat gcgttttcaa tggcgatatc gcgaatatca ccttcaatga
     3061 cgtctactgg cgtaggggct ttataggcgt caatatgacg acggcagcgt tcaatcatcg
     3121 ccggggagtt gtcgatggca ataattttgc aattatcatg atgaatgttg cgacgcaccg
     3181 agagcgtcgc cgcgcccaga gaacaaccca gatcgtaaac ctgcgtacca ggttgaacga
     3241 agcgctcggc taacatacta atcatggaaa taatattgga atagccggga acggaacgct
     3301 ggatcatatc cgggaagact tcagctaccc gttcatcaaa ggtccagtcg cccagtctgg
     3361 cgataggggc agaaaatagc gtgtcgcggt gagacataac gtaagaatcc gggaaaaaga
     3421 aagtggcgta ttgtgcgcta acgcagggag aaaaccaact cccagggcat ataccaaaga
     3481 ttcgccagca ccatcagcaa cagcgcacac caggtggcgc tcatgccgga acgtcgccag
     3541 c
//
LOCUS       DAJVOY010000264         3465 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVOY010000264 DAJVOY010000000
VERSION     DAJVOY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.267"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            577..1293
                     /locus_tag="OQL90_003985"
     CDS             577..1293
                     /locus_tag="OQL90_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370571.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1845..2249
                     /locus_tag="OQL90_003986"
     CDS             1845..2249
                     /locus_tag="OQL90_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370572.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2312..2946
                     /locus_tag="OQL90_003987"
                     /pseudo
     CDS             2312..2946
                     /locus_tag="OQL90_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2988..3197)
                     /locus_tag="OQL90_003988"
                     /pseudo
     CDS             complement(2988..3197)
                     /locus_tag="OQL90_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3262..>3465
                     /locus_tag="OQL90_003989"
     CDS             3262..>3465
                     /locus_tag="OQL90_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1370573.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLNTPGSRTVAELESEVM"
BASE COUNT          966 a          729 c          791 g          979 t
ORIGIN      
        1 ttgtgggcca taaatattcc aaaaaaatag cccgctccgt ttattaccgg aacgggcgag
       61 agcatgacat tgtctgtaat tataatgaga ctgctgattc tacttatttt taatttcaag
      121 aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa tattgtcatt
      181 ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacgtccct ctcatcgaac
      241 cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt cacgttcggg
      301 cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata ccagtagatt
      361 accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg ttgaccaagt
      421 tttgttcact tgtctggttt tgatagtagc attctgcaga gtattttgat atgataaata
      481 tgcattaaca atgatattat tgttaatttc ctagcatgaa aattaaaaga tcaacttttt
      541 atctcgaata ttttttacat tatcagttgg tttcttatga atgacaattc attgcttaga
      601 aattcttcat tattcatcgc ctatatggga tgtgtagggt gggtcagtgc ttattcttat
      661 ggctggggaa cctcttttta ctatggattt ccatggtggg ttgttggtgc aggtttggat
      721 gatgttgcca gaagtttact ctatgctatc atagtaatgg gtatactttt taccggttgg
      781 gggattggta ttttattttt tcttcttata aagaaaagaa gtaaaataca agacttgagt
      841 ttttttagac tattttttgc tattacccta ctgtttttcc ctgtaatatt cgaactttta
      901 attctcaaac aatatttcat acttccatta tcactttcat gcattattag ttcattggtt
      961 atttcaataa taattcgcat atatggacgg atcttttctg tatcatgttt ttcagatatc
     1021 ccttttgtta gagaacaccg cattaagtta attatggctg gttttttggt ttatttttgg
     1081 cttttttcct ttcttgttgg ctggtataaa cctcaattaa aaaaagaata tcagatgctt
     1141 tgttataata atagttggta ttatgttctt gctcgttatg attcacggct cgttttgtct
     1201 agttcattta aagatgacag taacagattc ttaatattta atacagaaca gtcgggattt
     1261 tatgaaatca atgatgttta tgtcagaaag taataatcct ctcccacaaa gtgaattaaa
     1321 cgaatatata atgcgttcga agtatatgta ttgagctgtc atactgagaa acaaacctta
     1381 gggcagcaca attaaagcca gaaacaacta tgcaagaaag ttactaaata agtgctcgga
     1441 acagtaaaac actcaggggg gactcagtcc agattatgta atcagataaa ttgccaaaca
     1501 tcacaaatca ccagcaggac tgagtgatgc cgagcattgc accaataccc cgtgatgaac
     1561 gatgcctgat gcagaaagct ccctataaaa caaagaacaa aaactacgac cggcgattca
     1621 ccgtcatact gatgctgcat cgtggtgaca ctgtctgtca ctttcatgtt gactgaacaa
     1681 ctctgctgcg aagaaggtgt accgccggcg ggatttgatt gtccggcgtc tagcttccac
     1741 ttccctgatg gaactgaaag ttgttatggc cggtcagctt gccctgtccg caggacatga
     1801 ggtgctgtga cggacagcac cagtaacgga aagtgacaat aaaaatgaat actgcgctga
     1861 tagttgcact aatgtgtatg tggtatgcgg tacccgctgc tgcaaaagag actttactgg
     1921 ctatgccacg taattcaaca gaacattgct atgcggagat taatgtgcac ggaccatatg
     1981 gggtgtattt ccgagtggtc ccgcatcccc ccgggggaaa gagctgggtc gaatgtaaca
     2041 gtgactacta ttactctgat aaaccaccgg gagtacagat actcggaacc cgtgcagggt
     2101 gtcgggtata cggaatctgc gggaccacct cgacactgca tgtggctgga agaggggttg
     2161 tgtgtattaa aaatatatgc agtccacgtg ggatgataat tcacaggata cggaaacgtc
     2221 ctgtagtggc tgtaagcgat gagatgtgat cttatccagc aatagtggac acgcggctaa
     2281 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagcaaaaa
     2341 aacacgtaaa cagcattcgc ctgaattttg cagtgaagcc ctgaagcttg ctgaacgcat
     2401 cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct atgcgtggcg
     2461 cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg ccgctgaaaa
     2521 tgcccgcctg aagcggcaac tggcggtgca ggcagaggaa ctggctatcc tccaaaaggc
     2581 cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     2641 gagttcagca tcaaggcaat gtgccgcgcg ctccgggtgg cccgcagcgg ctggtatacg
     2701 tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca ctgcgacagc
     2761 gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc acgcctgacg
     2821 gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc
     2881 cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat
     2941 ggcctgaggt gtactggcaa tagcggacac caccatttgt tctttttcta agcagtcatc
     3001 tgataatatt ttatcctgaa ggctgccggg gagatattcc ccagacgaga gtgacgacgc
     3061 tgacgattgt agaaaatctc aatgtattcc cgtattactg agatggcctc atcccggctc
     3121 ttaaaacgat agtggctcag gctttcattt ttcagcgttc cccagaagct ttccatcgga
     3181 gcgttgtcgt aacagtttga ggtagcctga gtttaacgga cactccttcc tgaaatagaa
     3241 tggcatcaga aggagccaat aatgagcaga aaaacccaac gttactctac ggagtttaaa
     3301 gccgaagccg tcaaaacggt tcctgaaaat cagctttcga tcagtgaagg cgcttcccga
     3361 ttatctgttc ctgaaggcac tttaggacaa tgggttaccg ccgcaagaaa agggctcaat
     3421 acaccaggct cccgcacagt ggctgagctg gaatctgaag ttatg
//
LOCUS       DAJVOY010000265         3409 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVOY010000265 DAJVOY010000000
VERSION     DAJVOY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.268"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(19..321)
                     /locus_tag="OQL90_003990"
     CDS             complement(19..321)
                     /locus_tag="OQL90_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370574.1"
                     /translation="MLEIVLSPVKAQQFTVTLDAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(321..1031)
                     /locus_tag="OQL90_003991"
     CDS             complement(321..1031)
                     /locus_tag="OQL90_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370575.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1028..3049)
                     /locus_tag="OQL90_003992"
     CDS             complement(1028..3049)
                     /locus_tag="OQL90_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1370576.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSQVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(3033..3215)
                     /locus_tag="OQL90_003993"
     CDS             complement(3033..3215)
                     /locus_tag="OQL90_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1370577.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          796 a          992 c          816 g          805 t
ORIGIN      
        1 ttttacgttg tctgtagctc atatcagttc ctcaggtaac agccagtaga gccggtaccg
       61 ggatccgagc ccccgccagt cagggtcact attcccctcc gtgtcggtaa aaaacaggtc
      121 cccctgaaac ggcaggtatc cgtaccggac aatccggtta ttattcagac acagcacgcc
      181 atacaggcac ggttcaccat taacaatgat atcgatatac atccccgtag tacgctgatt
      241 caggcgaatg gtgcagacct gagcgtccag tgtcaccgta aactgctggg ctttaacggg
      301 agataaaaca atttccagca tcaggtgatc cccctgttcg tggcacttcg tctgtcagcg
      361 tctgacggtt gtgtcaccga cgccgtaact ggctgcgttt taaccgatgc ggcccctttt
      421 gctttatcgt tgtccgtggg agacgactta tccgtacttc ccactgacac ctctcctgta
      481 ttcattacgg tctgaaatac cgcagtaacc gtcagtaagg tcggaccatt attacttcgc
      541 attcggtagt catatttcac aaggtcgcag gatgtccatg tcttgtccgg cgtctcaata
      601 tcgtagagtc ctgccgtggt acgcatccgt tcaagcgttt ccagcacatt cgtccgcgag
      661 gtggtggaaa aatttgtcag gttcgggacg gatccggaaa aggccgtcca cccctctaca
      721 atgaaagtca catgcagttc cggcgatctc tggattttat taaaggtggt ataagccccc
      781 tgctccacgg ggacggtgga aacagaagcc tccgcgccca cctcaacgac aacaaaagaa
      841 tccggggaga aaggtttgcc acccttctga tgggcacctg ccggatcatt ccgtgcgtaa
      901 taaataccga atgacggtgc cagtacacta ttaatgagtc ccaggacacc gccgccacga
      961 actgcactca gtacgttact ttcactgagc gaaaagttat tcagggaaat attatcgaaa
     1021 gaaaaactca tcctgttacc ccgctggaat aaaccgacac aagcgccgaa ttcgtgatac
     1081 gccgacgtgc gtcatcggta atacccttca cattgtccga cgttgtggtg acattcagtg
     1141 tcccgatatg cgtggtttcc gttacggtgg actgcgatac aggcgcagga tggagcgacc
     1201 gtatggccat tgccgcaccc ggataaggca ggttcgccag cacccgcgga atatagttgc
     1261 gggtctcctc cggagcagca gccagtccct tacgctgaac atttccctct ccccagttat
     1321 atgccgccag agccttagcc agatcgccat gaaaaaaacg tatcaggcca ccaagttttc
     1381 tcgcggcggc atcagcggct ttttcgggat caaaggcatc gttccccctc agaccaaatt
     1441 cactggccgt cttcggcatg aactgaaaca gtcccatcgc accagcaggt gagacggcaa
     1501 actgattacc acgggattcg gtgatcgcaa cgctgcgcag cagtccgggc ggcaggttat
     1561 atttttcctc cagttgagac agtttcggtt gcagccagcc taacagggct tccccggtct
     1621 tcgtcggtcg ctggcggttt tgcatggcat taccgagttt ttcctgcgtc gcacgcatac
     1681 cctgtagcca ggattcaccg aaggctgctt ccctcccggt tgcctgagaa gcctgagtat
     1741 ccagcatgcc ctgctgccat actgtgggtg attgtgcccg ggtgatgttg ccaggctttt
     1801 ctccggcatc cagttttgtc tgatacgtct ccatctcttt cttattgaaa aagaaagtcc
     1861 cgtctgaagc ccagaaaaaa ccatgtgaat ccagccagtc cttattttcc ctgccaacaa
     1921 tggatgtcag taatccgtca acaaccgggt aaagcgccgt tatcgcaaaa agaaggcctc
     1981 cggggccact gagggctttt gtcagccccc gcacccatga cgccactttc agcccgatca
     2041 gcgtaataat gacattctgc cagcccccca gttctccggc agccttattc gccagggagg
     2101 ccactgactc aacgtcattc agaaatgtgg tgataaacct gttcacttcc tccggatgtt
     2161 gctgcatcca gtcaccgagt gtctccagcc agtggctgag cttcagcgcg tacggcatca
     2221 acgcggtgct aatcgtcatc ccgatgccgg accaggtctg atcgagctca acaagctctt
     2281 ttcgtagctt acgggccagc gctgtacgcc ccggcgtaac ctgcgaacgg gaagtaaatt
     2341 cgtccacatc tttcagcgca tgaccggagc caagaaactg ctgtccggca taactgaagc
     2401 ccagcgcgtt gccgtaggca gtttgttcgg actttgtcag tcgcggaaaa ataaccgcca
     2461 gcttgcgcat gatggcttca gtgctgtcag tatttaaatc aacactaatg cccgccctgg
     2521 ctgcaacctg aaacaaatcc tgtaacacag gatcaaagga ccttccggct ctgaaggccg
     2581 cttttgcatt tgcaagccgg gaaaatgccc cagacatttc cccttcatca acattatttg
     2641 cccgcgcagc ccgtaaccat ccgtccagat ttctggcctt catgccaaaa gcatcggatt
     2701 ccacggacag acggttcaga tcacgggcaa aacctgttac caggttcttc agccccccca
     2761 gcgtcagact gacacccgca agagccagaa tctcgctacg gatccctgaa aaaaaacggg
     2821 acgcccgttt cccggcatct tccattcgac gggccgtttt atcagcatct tttccggttt
     2881 tttccagtga ttcactggtt ttcttctgtc cctgactgaa tgtgtcagcg acaccttcca
     2941 tcaccgtcgt gagccggtta agggcagcat gtactgcctg ttccccggca gtaaagtccc
     3001 tgtcatcaat cccgagggac agaacaagct catcaagtac ctgcgccatt tctgttctcc
     3061 tgtatcacct gttcgttatg ggcatccaca tgaattattt ccagcagatc ccataagtcc
     3121 tgcacaccaa ggacagaatc cagttcagct tttgaagcct taccggagga gataacggtc
     3181 gcaatggtac ggggaacatt aacgtaatcc accaccccga acggtctgtc ggggtcgaga
     3241 taacgcgggg ggatatctag ctgacgacgg tagcgaaaaa atccacatgc agcttaaaga
     3301 cctccgcacg cagattaaga cgggtaatga tttcttcaat atcctcttca acaagcggtc
     3361 tccggatgct gcgttttttc ggatcgggaa caaactgcac acagtccat
//
LOCUS       DAJVOY010000266         3355 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVOY010000266 DAJVOY010000000
VERSION     DAJVOY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.269"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1088
                     /gene="nadB"
                     /locus_tag="OQL90_003994"
     CDS             <1..1088
                     /gene="nadB"
                     /locus_tag="OQL90_003994"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCS1370578.1"
                     /translation="VGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSGD
                     GIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPDF
                     DERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGIDL
                     TQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECLV
                     YGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIVR
                     TTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRGL
                     HFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="OQL90_003995"
     CDS             complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="OQL90_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1370579.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1942..3276
                     /gene="srmB"
                     /locus_tag="OQL90_003996"
     CDS             1942..3276
                     /gene="srmB"
                     /locus_tag="OQL90_003996"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1370580.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT          836 a          916 c          894 g          709 t
ORIGIN      
        1 ttgtaggcgc gtgggtatgg aaccgaaata aagaaacggt ggaaacctgc cacgcaaaag
       61 cagtggtact ggcaaccggc ggtgcgtcga aggtgtatca gtacaccacc aatccggata
      121 tttcttctgg cgatggcatt gctatggcgt ggcgcgcggg ctgtcgggtt gccaatctcg
      181 aatttaatca gttccaccct accgcactgt atcacccaca ggcacgcaat ttcctgttaa
      241 cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc
      301 ccgattttga tgagcgcggc gaactggccc cgcgcgatat tgtcgcccgc gccattgacc
      361 atgaaatgaa acgcctcggc gcagattgta tgttcctcga catcagccat aagcccgccg
      421 attttattcg ccagcatttc ccgatgattt atgaaaaatt gctcgggctg gggattgatc
      481 tcacacaaga accggtaccg attgtgcctg ctgcacacta tacctgcggt ggtgtaatgg
      541 ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag gtgagttata
      601 ccggcttaca cggcgctaac cgcatggcct cgaattcatt gctggagtgt ctggtctatg
      661 gctggtcggc ggcagaagat atcaccagac gtatgcctga tgcccacggc gtcagtacgt
      721 taccaccgtg ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata
      781 actggcacga gctacggctg tttatgtggg attacgtcgg cattgtgcgc acaacaaaac
      841 gtcttgagcg cgccctgcgg cgtatcaata tgttgcaaca ggaaatagac gaatattacg
      901 cccacttccg cgtgtcgaat aatttgctgg agctgcgtaa tctggtgcag gttgcggaat
      961 tgattgttcg ctgtgccatg atgcgcaagg agagtcgtgg cctgcatttc acactggatt
     1021 atccggaact gctcacccat tccggtccgt cgatcctttc ccccggcaat cattacataa
     1081 acagataaaa agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac
     1141 gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc
     1201 gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca
     1261 tgcttaatgc cagctccgta aaaccattac caatctgctc tggcagcact acgcagaaaa
     1321 atccctcttc ggtaatgcac tccgccgcac aggtcagcag cgatgggtga tcaagcgtag
     1381 tggtatagcg agcctgttcc cgttgaggga tcgcgcactc tactccctgc tgatagtaag
     1441 gtgggttact gatgattaaa tcgaagcgta ctgtctgctg tgtgagccac tgctgaatat
     1501 ccgccgtatg gacgttaatc cgctctgccc acggggactg gttgatattt tcctgcgcct
     1561 gcgcggcagc ttcactttcc agctcaacag catcaattat cacgctgtca ctggttcgct
     1621 gcgccagcat cagtgccagc aacccgctac ccgcgccgat atcaaggcaa cgtttcaccc
     1681 cagccaccgg tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacagc
     1741 gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaagc acggatgtag
     1801 actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat
     1861 acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag
     1921 cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa
     1981 agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc
     2041 gccattccgc ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac aggcaccggt
     2101 aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc gcgtaagaaa
     2161 tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg agctggcgat gcaggtggcc
     2221 gatcatgccc gcgaactggc gaaacatacg catctggata tcgccaccat taccggcggc
     2281 gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg
     2341 accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg
     2401 ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgctcagga tatcgaacat
     2461 attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac gctggaaggc
     2521 gatgcgattc aggactttgc cgagcgtctg ctggaagatc cggtggaggt ttctgccaat
     2581 ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgacctggaa
     2641 cataaaaccg cattgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg
     2701 tttgtgcgta agcgtgaacg tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc
     2761 aacaactgct atctcgaagg tgagatggta cagggcaagc gtaatgaagc gattaaacgc
     2821 ctgaccgaag gccgcgtaaa cgtactggta gcaaccgatg ttgccgcgcg cggtatcgac
     2881 attccagacg tcagccacgt ctttaacttc gatatgccgc gcagtggtga tacttatttg
     2941 caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcgatttc gctggtggaa
     3001 gcccatgacc atctgctgct tggcaaagtt ggccgttata ttgaagagcc gataaaagct
     3061 cgcgtcattg aggaactacg cccgaaaacg cgtacgccaa gcgagaagca gaccggcaag
     3121 ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaag
     3181 ccgcgggtga aaaaacgcca tcgcgacacc aaaaatattg gtaagcgccg taaaccgagc
     3241 ggaacgggcg tgccaccgca aacgacagaa gagtaatctt aattccgggt ttaagcccgg
     3301 aattaaaagt gctggcaaac gcaaaactgc ctgatgcgct acgcttatca ggcct
//
LOCUS       DAJVOY010000267         3319 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVOY010000267 DAJVOY010000000
VERSION     DAJVOY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.270"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..211)
                     /locus_tag="OQL90_003997"
     CDS             complement(<1..211)
                     /locus_tag="OQL90_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370581.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVCISLQELQLELMVEYALFF"
     gene            complement(214..981)
                     /locus_tag="OQL90_003998"
     CDS             complement(214..981)
                     /locus_tag="OQL90_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370582.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(984..1694)
                     /locus_tag="OQL90_003999"
     CDS             complement(984..1694)
                     /locus_tag="OQL90_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370583.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1710..3215)
                     /locus_tag="OQL90_004000"
                     /pseudo
     CDS             complement(1710..3215)
                     /locus_tag="OQL90_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          935 a          797 c          728 g          859 t
ORIGIN      
        1 tgaaaaaaag tgcatattca accattaact caagttgtaa ttcctgaaga gaaatacaca
       61 caaccaggag cggaaaggag aatattattt ctgccagaaa agttaacgtg aatataagta
      121 cggctaagac aataaccgtc ggccatacca ataatggcac gataacaaaa ccgagaatta
      181 cagggggaat ataaagccgg aatctaatca tttttatttt cctagtgatt cggatgatgc
      241 gtcatacatc gcgcataaac actcatcaat tatccagtca tcttcccaga cctgatattc
      301 tgtcattggt tcgggctgtt ttagcgggtt ctcaatcgct tgccgcgctc tttctttcag
      361 taaaggatga ttatttctag cccaggcatt gatttgggtg gtaatgacgt tatcgatatc
      421 gtcctggtta tctctgatat aatcctcaca atatctgttc aataaacagg caagagaaac
      481 ggtcgaccaa aagccgggaa ctgaattaat ggcatccagc aatggcgcaa gagcgatatc
      541 ggcacggaaa aacatttcca gcatatactg taattcgaca gcttcatcgc cggataccca
      601 atcagtcata tactggcagg caaatcgctg taaaattgcc cgttcatccg cacgccagtt
      661 ttctttgttg gcaaggttga tacggcgcag agaaaaaact acgtgaatat tgtcaaaatg
      721 cccctgtgca acaaattcca gtaaacgcgg cagaaaatag cggacctcat cgctattttg
      781 cggatcaggc cctgggctac tattccaggc attgattaag gaatacggga tcgctcgtaa
      841 cgatgtactc cgtaatgcct tttgttcctg ctgactcagg caatattcac aacaaaccac
      901 aaatttctgc ggcacccgat aacgacgaaa ggtgtgataa atctgctcaa ttaatgcctg
      961 catggcgggg cagggttgca tagttacccc ctcgcccgct gatcgcgtgt aaacggttga
     1021 tagacaaaac gagcattcgc cgcgttcagg cgaataaggg cgctacgcga aagggaaaag
     1081 ctggggcgaa tgcaactatt gcagtctcga cagctttcct ctgcgcaatt ttccactgag
     1141 taatgacgcg tgtagagttt cagttcggcc tgcttatcgc gaaatccgcg taaactgtta
     1201 ttgttatgaa atttccaggc atctttaaag acctgaaaga tatagacatc ctcaatccag
     1261 gagtcacatt ttttaagcgg gcgctttgct attatcaccc gctcatccgg atttagcatc
     1321 gccgtaaatt gtacggtgac gtcatttgtc gtgttcaggt cacattgaac atctggtaca
     1381 tcactgatat aggggtcaaa tccaaaatca gcaccacagg cacaaagacg gctaatggtg
     1441 gcggtatcga gggaaaatcc cggccatact ttgctggagg ttaagccaat atttaatgtg
     1501 acatcatatc gaagcaatcc ctcaagaccg tcagcaccga cgaacttttc cccttcatcc
     1561 agggcctggt taataagttg ctgaacaatg ccttcatccc agtatgccga tttgcagttg
     1621 gcaatcgtaa tatccacgcc tgtgaaggga tcagtaccgg caaggcttgc aaataatgta
     1681 ttaactaatt ccatttttat tatccagctt taaggtagcg tcttatacat cgggacataa
     1741 cctacatctt ccaccaaact ctgtccctgc ggcgttaaaa accactccaa caatttttgt
     1801 gtctctgacg tcgtattttc ccgcgttacc ataaaggcat cgatgatata gggatatttg
     1861 ccgttgcgaa tgttttcggc agtcggtgca ataccgttaa tcgctagcag ttttatattt
     1921 ttatcggcgt tcatttgtgt tgcgtaataa cggaaggtgt aacctattgc gttgtttgta
     1981 ttacggtatt cggcaacgac tttaatcatt ccctccatca tacttgccac ttctgtttcc
     2041 tgcggcgaga tcatgcggac atttttcatg acctgggatt gcatcaccgt ctggctacca
     2101 gagtcttccg ggcgttgcta ggtctggatc tcctgatcgt taccgccaac agtacgccag
     2161 ttggtaattg caccactgaa gatgtcacgc acctgttgtt cagtcaggga attaaccgga
     2221 ttatccgcat tgacgatgaa aacaaacgct tcacgggcaa aaggcgtgta cagcaaagta
     2281 atgcccgatt cctcagcgcg ttttttctgc ccaccggaag gttgcgccac aaaaataata
     2341 tcagcatcac ctttaacaat cctgttatac gcatcggggg tacgagagct ctccagatat
     2401 tccctggtgt gaaaatcttc cggtataacg ctcaaagcat aaaatgcaga ggcataaatt
     2461 gggtacgccg ccgtagcacc atcgagtcgc ggccagtttt gcgtaaactg aatttgcggt
     2521 ttaccacgca gtggtgtcag ttgattattg agtttatcgg gtcgccaggc ccaggtatca
     2581 atattttcgc gcacaccgac gccgggattg agctgtttat ataaataacc ctgataacca
     2641 gcgacaaaac caagcatgag aaggataaac aggagggaat tacggcaacg tagcccggat
     2701 gtaccggtta aaggatgtct ggttggccgg ccgtacccca gcgaaaaact gatatgcgcg
     2761 ccaacgggta taatgactat ccaccagtac tcacctccga ctactgagac taacaaaacg
     2821 cccagccagg ggagcataag atcagccaac caacccgatc ccatctggtg aaattgataa
     2881 tctgccagat gcagcgcgat tatccagcca accaggatta ataaaccggg tactaaaaaa
     2941 agacattgtc gccagacggc tatttctctt ttttctggtg cactatcgcc tgcccatact
     3001 cgccccgaga aaaatagcat taatgcgccc agcgccagca taaaaatgtt gagcagaagg
     3061 taacttgttg gcgtgataag gatattacaa acttcaataa taccggcaag tccgacaccc
     3121 agcatagcaa caaccgccgc caaaaattgc gtcagtatgg ggatgccgaa aaaagtcatt
     3181 accagcgagg acaaaatcca ttttctgttt tgcattattc tttccattct ttttgaatgg
     3241 tgaaattata ctccccgagt ccccttgccc cttctggaca cttttccgaa atgatggcgg
     3301 aaaaaaacgg gaccctttg
//
LOCUS       DAJVOY010000268         3313 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVOY010000268 DAJVOY010000000
VERSION     DAJVOY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.271"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            385..1104
                     /locus_tag="OQL90_004001"
     CDS             385..1104
                     /locus_tag="OQL90_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1370584.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            1115..2542
                     /locus_tag="OQL90_004002"
     CDS             1115..2542
                     /locus_tag="OQL90_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS1370585.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2535..3230
                     /locus_tag="OQL90_004003"
     CDS             2535..3230
                     /locus_tag="OQL90_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS1370586.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          870 a          692 c          929 g          822 t
ORIGIN      
        1 ctaaattgct tcaattaaat aaaatcccga ctattacttg atacgtgatt attgtcggtg
       61 attttgtttg tacaccatca gtgtatatct caataacccc tgaataagta gctctgaata
      121 ggtataaggg atgtggccat ttttaaatgg atttcttatg ccaaaaatga tcgtcgattt
      181 ctccataaaa tgtgagcgat gccgaaagaa ataaaattag ttattgcatt tgacgtttgg
      241 gtgaaagatt atcatttgtc atacaaatga ggggtagtat gttgctagcc aattaaaaaa
      301 gaacgccata tttattgatg attgatcccc cggtgggggg gaaacatcgg gcataaatgg
      361 gcatgaagta atggagtatt agttgtgaat gtcaatttct ttgtcacctg tattggtgac
      421 gccctgaaat caagaatggc acgagactcc gtgctgctac tggaaaaact cggctgtcgc
      481 gtaaatttcc cggagaaaca gggatgctgc ggtcagcctg cgatcaatag cggttatatc
      541 aaagaagcga ttccagggat gaaaaatctg atcgccgcac tggaggataa cgacgatccc
      601 attatttcac cggctggctc ttgcacctat gccgtaaaaa gttacccgac gtatctggcg
      661 gatgaacctg aatgggcatc acgtgccgaa aaggttgccg cgcgtatgca ggatctcacc
      721 tcttttattg ttaataaatt aggggtagtc gatgtaggtg ccagtttgca agggagagct
      781 gtgtatcacc catcttgtag cctggcccgt aagctgggag tgaaggacga gccacttacg
      841 ctgctgaaaa atgtgcgtgg actggagctg ttgacctttg ctgaacagga tacctgctgc
      901 ggatttggcg gcacgttctc ggtcaaaatg gccgaaatat ccggcgagat ggtgaaagaa
      961 aaggttgggc acctgatgga agtccgccct gagtatttaa ttggtgctga cgtgagttgc
     1021 ctgctgaaca tcagtgggcg attacaacgg gaagggcaga aagtcaaagt gatgcatatt
     1081 gctgaagtgt tgatgagccg ctgaggatat aaagatgtcg atcaaaacca gtaatacaga
     1141 ttttaagact cgcatccgtc agcaaattga agatccgatc atgcgcaaag cggtggcaaa
     1201 cgcgcagcag cgtattgggg caaatcggca aaaaatggtc gatgaattgg ggcactggga
     1261 ggagtggcgc gatcgggccg cccagatacg tgatcatgtt ctgagtaatc tcgacgctta
     1321 tctgtaccag ctctcagaaa aagtgacgca aaacggcggt cacgtctggt ttgcaaaaaa
     1381 caaagaagac gctacccgct acattttaca ggttgcccaa cgcaaaaatg cccggaaggt
     1441 ggtgaaatct aaatcgatgg tgaccgaaga gattggtgtc aatcatgtgt tgcaggatgc
     1501 tggcattcag gtgattgaaa ccgatctggg tgaatatatt ctccagctgg atcaagatcc
     1561 gccatctcat gttgtggtcc cggcaattca taaagatcgc catcagatcc gtcgagtgct
     1621 acacgaacgt ctgggctatg aggggccgga aacgcctgaa gcgatgacct tattcatccg
     1681 gcaaaaaatc cgcgaagatt tcctcagtgc tgaaataggt attaccggct gtaatttcgc
     1741 ggtggcagag acaggttcgg tatgcctggt gaccaatgaa ggtaatgcgc gaatgtgtac
     1801 cacgctgcct aaaacgcata ttgcagtgat gggaatggag cgtattgccc ccacgtttgc
     1861 cgaggtagat gtattgatca ccatgctggc gcgcagtgcc gttggtgcac gtttgacggg
     1921 atacaacacc tggctgacag gaccgcgcga agcggggcac gttgatggtc ctgaagagtt
     1981 tcatctggtt attgtcgata acgggcgttc tgaggtgctg gcctctgaat ttcgggatgt
     2041 gctgcgctgt attcgctgcg gggcttgtat gaatacttgt ccggcatatc gccatattgg
     2101 cggtcatgga tatggctcta tttatccagg gccaattggt gcggtgattt ctccgctact
     2161 tggcggctat aaagatttta aagatttacc ctacgcctgc tctttatgca ctgcttgtga
     2221 cagcgtgtgt ccggtgcgta ttccgctgtc aaaactgatt ttgcgtcatc gtcgggtgat
     2281 ggctgaaaaa gggatcaccg caaaagcaga gcaacgggcg ataaaaatgt tcgcttatgc
     2341 caatagtcat ccaggattgt ggaaagtcgg gatgatggcc ggcgctcatg cggcaagctg
     2401 gtttatcaat ggcggcaaaa caccactcaa atttggcgcg attagcgact ggatggaagc
     2461 acgcgatctt cctgaagctg acggagagag tttccgtagt tggtttaaga aacatcaggc
     2521 gcaggagaaa aagaatggat aatcgaggcg aatttttgaa taacgttgct caggcactgg
     2581 gtcgcccgct gcgacttgaa ccgcaagcag aagatgcgcc gcttaacaac tatgctaacg
     2641 agcggcttac ccaacttaac caacagcagc gctgtgacgc gtttattcag tttgccagcg
     2701 atgttatgtt gacgcgctgt gagctgacca gcgaggcgaa ggcggcagaa gctgcaatac
     2761 gtctgtgtaa agagctggga gatcagtcgg tcgtgattag cggtgacacg aggctggagg
     2821 aattggggat tagtgaacgt ttgcagcagg aatgcaatgc cgttgtttgg gatccggcga
     2881 aaggtgccga gaatatctcg caggcagagc aggctaaagt gggtgttgtg tatgctgaat
     2941 atggtttaac cgaatcggga ggcgtggttc ttttttccgc cgccgagcgc gggcgttcat
     3001 tgagcctgct cccggaatct tctcttttta tcctgcgtaa aagcacaatc ctgccgcgtg
     3061 tagcgcaact cgcagaaaaa ttgcatcaga aagcgcaggc cggtgaacga atgccgtcct
     3121 gcattaacat cattagcggc cctagttcaa cggcggatat tgagcttatc aaagtcgtcg
     3181 gggttcatgg cccggtgaaa gcggtgtatc tgattattga ggattgctga gagagatatg
     3241 agatctgaac ataaacctga agaaggcata cgaggttgaa aatagcgtta aggacaacct
     3301 gacttaaccc cgt
//
LOCUS       DAJVOY010000269         3280 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVOY010000269 DAJVOY010000000
VERSION     DAJVOY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.272"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..2217)
                     /gene="metH"
                     /locus_tag="OQL90_004004"
     CDS             complement(<1..2217)
                     /gene="metH"
                     /locus_tag="OQL90_004004"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1370587.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEAQDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTDNAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEAS"
     gene            2417..3241
                     /gene="iclR"
                     /locus_tag="OQL90_004005"
     CDS             2417..3241
                     /gene="iclR"
                     /locus_tag="OQL90_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1370588.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT          735 a          922 c          862 g          761 t
ORIGIN      
        1 gctggcttca ataaacggtt cgagatacgc caccgcctgt ttcatgacgc gcgccgattt
       61 gaccacctgt ggcaggaaca ttttgccttc gccaaacaga tcgccgacca cgttcatgcc
      121 gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc
      181 ggtatcctgc tcgataaact cggtaatgcc tttgaccagc gagtattcca gacgtttatt
      241 cacttcccac gagcgccact ccgcctgctg ggcgttatcg gtgtcgtcgg ttttgctgcc
      301 gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcgtcacgac gattaagaat
      361 cacatcttcc accgcgtcgc gcagttcagc gggtaggtcg tcgtaaatcg ccagttgccc
      421 cgcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga acactgcgtg
      481 aatggcttcg cgcaccggat cgttgccacg gaatgagaaa gaaacgttag atacgccgcc
      541 ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga taaagtcctg
      601 cgcgtagttg ttgtgctctt caataccggt ggcgaccgcg aagatgttcg ggtcgaagat
      661 gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat
      721 ctcgattttc cgtgcgcgag tatcggcctg tccctgttca tcaaaggcca tcaccaccac
      781 tgccgcaccg tagcgacgca acaatttcgc gtgatggata aaggcgtcga cgccctcttt
      841 catcgagatt gagttaacaa tgcctttgcc ctgaatacac ttcagccctt tttcgataac
      901 gtcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat
      961 cagattgaga aaacgcacca tcgccgcttc ggcatcgagc atcccttcat ccatgttgat
     1021 atcgataatc tgcgcgccgt tttccacctg ctgacgcgcc acatcctgcg cctcgctgta
     1081 tttctcttct ttgatcaggc gcttgaactt agcagagccg gtgacgttgg tgcgttcacc
     1141 cacgttcaca aacaggctat cgtcgccaat gttcagcggc tccaggccag acaaacggca
     1201 ggctacggga atttccggca gtttgcgcgg cgctaatcct tctactgcac gactcatcgc
     1261 tgcaatatgt tgcggcgtgg tcccgcagca accaccgacg atattgagaa agcccgcttg
     1321 cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtatt caccaaaggc
     1381 gttaggcagc ccggcgttcg ggtgggcggt gacgtagcat tccgcaatcc gtgatagttc
     1441 ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa aggttagcgc
     1501 ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc
     1561 ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca gcgcttcaaa
     1621 ctccgttttc accgcaaaaa ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat
     1681 caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat aggccgccac
     1741 cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccggag aaatagacgc
     1801 cgtgcggttg gttgggccga gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc
     1861 ggtccactcg tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga
     1921 cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc
     1981 gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt
     2041 gctgagcacc agcaggtcgt tgttgccttt gagatcgcat ggccagtcgg caaagcgttc
     2101 accacgaaaa tcggcttcgt tcagtcgata actctggatc atggtgccca taccgccgtc
     2161 cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact
     2221 tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac
     2281 gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg
     2341 atcagttcag caactattgc attagctaac aataaaaatg aaaatgattc ccacgataca
     2401 gaaaaaggag actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt
     2461 tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga
     2521 gtggattgcc gaatccaatg gcagtgtggc actcacggaa ctggcgcaac aagccgggtt
     2581 acccaattcc acgacccacc gcctgctaac cacaatgcaa cagcagggtt tcgtgcgtca
     2641 ggttggcgaa ctgggacact gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt
     2701 tctccagagt cgtaatttgt tagcaattgt tcaccctatc ctgcgcaatc taatggaaga
     2761 gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat
     2821 cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg gtaaattgcc
     2881 gatgcacgct tccggtgcag gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac
     2941 gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg tgtctcctgt
     3001 gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg acgatgagga
     3061 acatgcgctg gggctacgtt gccttgcagc gtgtattttc gatgagcacc gtgaaccgtt
     3121 tgccgcaatt tctatttccg gaccgatttc acgtattacc gatgaccgcg tgaccgagtt
     3181 tggcgcaatg gtgattaaag cggcgaagga agtgacactg gcgtacggtg gaatgcgctg
     3241 actttttctg gcgggcagag gcaatattct gcccatcata
//
LOCUS       DAJVOY010000270         3225 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVOY010000270 DAJVOY010000000
VERSION     DAJVOY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.273"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..907
                     /locus_tag="OQL90_004006"
     CDS             <1..907
                     /locus_tag="OQL90_004006"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1370589.1"
                     /translation="ANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNK
                     AWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDM
                     NISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCV
                     KVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVY
                     YSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALS
                     CEYTNNPDLSIANVISLVENAERLN"
     gene            874..1833
                     /gene="psuG"
                     /locus_tag="OQL90_004007"
     CDS             874..1833
                     /gene="psuG"
                     /locus_tag="OQL90_004007"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1370590.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1927..3177
                     /locus_tag="OQL90_004008"
     CDS             1927..3177
                     /locus_tag="OQL90_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1370591.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT          771 a          726 c          872 g          856 t
ORIGIN      
        1 ggcgaatatt gatgtcgccg gatattcaca tgaatcatta aattatgcgg attcaaatcc
       61 aggtaaaata aaatttacgc ctggtggagt cgggcgcaat attgcacaaa acctggcgtt
      121 gctggggaac aaagcctggc tactgagcgc cgtaggcagt gacttttatg gtcaatcgct
      181 gctaacgcaa accaatcaat ctggcgttta tgtcgataaa tgcctgattg tgccgggaga
      241 aaatacgtct agttatttat cattactcga taataccggt gaaatgctgg ttgctataaa
      301 tgacatgaat attagcaacg ctattacagc tgaatatctc gcacagcacc gtgaatttat
      361 tcagagggca aaggtcattg tcgcggactg taatatcagt gaagaggcac tggcatggat
      421 tctggataat gccgccaacg tacccgtatt tgtcgatccg gtttccgcat ggaaatgtgt
      481 caaagtgcgc gaccgtctaa atcagatcca cactctgaag ccaaaccgcc ttgaagcgga
      541 aaccctgagt gggattgcgc tgtcagggcg tgaagatgtg gcaaaagttg ctgcctggtt
      601 ccatcaacat ggcctgaacc gactggtatt gagcatgggc ggcgacggcg tttattacag
      661 cgatatcagc ggtgaaaata gctggtctgc gccgatcaaa accaatgtta ttaatgttac
      721 cggagcgggc gatgccatga tggcgggact tgcttcgtgt tgggtagacg gaatgccgtt
      781 tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct gtgaatacac
      841 caataacccc gatttatcga ttgccaacgt tatatcgtta gtggagaacg cagaacgtct
      901 gaattaaaaa tttcccctga attattacaa atttccccgg aagtgcagga cgctttaaaa
      961 aacaaaaaac cggttgtggc gctggaatcg accattattt ctcacgggat gccgttccca
     1021 caaaatgccc agaccgcaat tgaagtagaa gaaactattc gtaaacaggg cgctgtacct
     1081 gccactatcg ccattattgg cggcgtgatg aaagtgggtt taagcaaaga agaaattgaa
     1141 ttactgggtc gtgaagggca taacgtgacc aaagttagtc gtcgcgattt accttttgtt
     1201 gttgccgccg gaaaaaatgg cgcaaccact gtggcttcaa cgatgattat tgcggcgctt
     1261 gccggaatta aagtatttgc caccggggga attggtggtg tgcatcgcgg ggcggaacat
     1321 accttcgata tttctgccga tttgcaagaa ctggcaaata ctaatgtcac cgttgtttgt
     1381 gccggggcga aatctattct cgatttagga ttaaccactg agtatttaga aaccttcggt
     1441 gtgccgttaa ttggctatca gactaaagcg ctgcctgcgt ttttctgccg taccagctcg
     1501 tttgacgtca gtattcgtct cgacagcgcc agtgaaattg cccgtgcaat ggcggtgaaa
     1561 tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga acagtttgcg
     1621 atgccggagg aatctatcaa tgcagccata gatcaagccg tcgccgaagc cgaagagcag
     1681 ggcgttattg gtaaagaaag tacactgttc ctgctggcgc gcgttgctga gctgaccggc
     1741 ggtgacagcc tgaaatccaa tatccagctg gtattcaaca acgccattct ggcgagtgaa
     1801 attgccaaag aataccagcg tctcgcgggt taatttccga cgggcagaaa gccgtcgccc
     1861 gtactcatca agcataccaa aacctggcgc tgacgccggg tttactggca tgaagggaaa
     1921 atcattatgg atataatgag aagtgttgtg gggatggtgg tgttactggc aatagcattt
     1981 ctgttgtcag tgaataaaaa gagcatcagt ttgcgcacgg ttggagccgc actgctgctg
     2041 caaatcgcta ttggtggcat catgctctac ttcccaccgg gaaagtgggc agtagaacag
     2101 gcggcattag gcgttcataa agtgatgtct tacagtgatg ccggtagcgc cttcattttt
     2161 ggttcgctgg ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt tatcttcgcc
     2221 tttcgcgtac ttccggcgat tattttcgtt actgcgctca tcagtctgct gtactacatt
     2281 ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt tccagaaagc tcttaacatc
     2341 agcaaaatcg aatcttttgt cgcagtcaca actattttcc tcgggcaaaa tgagatcccg
     2401 gcgatcgtta aaccgtttat cgatcgcatg aatcgcaacg agttgtttac cgcaatttgt
     2461 agcgggatgg cgtccattgc tggttcgatg atgattggtt atgccggaat gggcgtacca
     2521 attgactacc tgttagcggc atcgctgatg gcgatcccag gtggtatttt gtttgcacgt
     2581 attcttagcc cggccaccga gccttcgcag gtcacatttg aaaatctgtc gttcagcgaa
     2641 acgccgccaa aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg gctgaaaatc
     2701 gccgctggtg tagcgacggt ggtaatggca tttgtcgcaa ttattgcgct gatcaacggc
     2761 attatcggcg gaattggtgg ctggtttggt ttcgccaatg cctctctgga aagtattttt
     2821 ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg actggagtga tgccaatctt
     2881 gcgggtagcc tgattgggca gaaactggcg attaacgaat tcgtcgctta cctgagtttc
     2941 tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa ccattgcgat tatctccttt
     3001 gcgctttgtg gttttgctaa ctttggttct atcggtgttg tcgttggcgc attttcggct
     3061 atttcgccaa aacgcgcgcc ggaaatcgcc cagcttggtt tacgggcgct ggcagcagca
     3121 acgctttcca acctgatgag tgcgactatt gccgggttct ttattggttt agcttgagtt
     3181 tcattgccgg atgtacgcgc ttattaagcg ttgcatccgg caatc
//
LOCUS       DAJVOY010000271         3217 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVOY010000271 DAJVOY010000000
VERSION     DAJVOY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.274"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..1000)
                     /locus_tag="OQL90_004009"
     CDS             complement(<1..1000)
                     /locus_tag="OQL90_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1370592.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCRLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETN"
     gene            complement(1262..2852)
                     /locus_tag="OQL90_004010"
                     /pseudo
     CDS             complement(1262..2852)
                     /locus_tag="OQL90_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT         1032 a          589 c          506 g         1090 t
ORIGIN      
        1 aattcgtttc atgatttcca gctaatacat gaacattttc attgatactc attatgcgtt
       61 ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg ccggtttggt
      121 cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aactaaataa ggctgaagac
      181 tttctaaaac attatctctt ctgttcaggt tactgattat tgtattaata ttagtgtcaa
      241 gttgctcatt aaagttttcc cttagcaata cgttgagaac ttcatcttct atagtaagca
      301 accggcaaag gaaattttct ccttcagtca tgattcccaa atatcccgta gcaaaaagcg
      361 cgtacaaaac tgcaccaact gaaccatcgg tatccccaaa ataggtcggc ctggtacttg
      421 tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg gggtttataa
      481 ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc cagatgggtt
      541 tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata ttattaagta
      601 aataaacaac actgcaccaa ttaacatcag tattatcaag agagcaaccg taatttttac
      661 tattaattgt cacaataaac tgataattat tatccctacg tagaatatga gcacgcatgc
      721 ccgatagcat actatatttt tctgaatgat ccgtttgtat ggtaaatgaa attttgtcat
      781 cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc acaacctcac
      841 cgagcattgt cctggcaata cttgatatat catcacctat accatttgca atatacccaa
      901 acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt gcgattgtcg
      961 ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc atattattca
     1021 ccgcgaggaa tacatagtta acattttatc ttatgacata cacctcatgt tcgatggaaa
     1081 aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat ttcttatttc
     1141 atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgtct ctacaatcga
     1201 ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca ctcatggagt
     1261 tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca cgttcgttag
     1321 gagctatctt ccaggaattt agccccatgg tataattatc aattttttga tgctgatcta
     1381 cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt ccacatttga
     1441 agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg tcatatacag
     1501 gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc atgtgttgtt
     1561 tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct ttcatctgat
     1621 taaatggaac ctgcaaagca cctagacaat aacattcatt ttcttcattt cttattattc
     1681 cgtgatgact gcttaaaact ttcgtttctg agtcataatt acaaacatcg agcgttttaa
     1741 ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca gtatagttac
     1801 cattgaaatt gatttcataa tttccagcca gaacgacgtc atttttattt atacgactat
     1861 ctttattact ctccatattc cgcatagaat ttagcaaagt aaggatatat ttatcaccaa
     1921 aaatagtact gaatctatcg cctgtatgat ccccaagaaa gatacaaggg gtaatcgcat
     1981 cgccttttaa aaaaacatgt tgatgcaact tattcaagat catgtctctc gtatctaata
     2041 aagtaaagag tttttccaat tttttatttt catatgcgaa tgaagccatg tcttcgtgtt
     2101 ttaataattc acataataaa ctccacccct tttccctgat acctatatgt cctgaaacga
     2161 gcaatgcata aagtacagcg ccaactcttc catctgtatc gccaaaataa gccgtgttgg
     2221 gattttgtgg gttaatcaca ccacactgtt tataaaaatc tatttttgaa aacacagaat
     2281 taacatagct agcaaactta tccttaggaa acgtctcttt agagacatcc caacgctagt
     2341 cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata gaaaggccat
     2401 taactttata aattatgtct gatttatctc cggagaaact aataaaataa taaccatcct
     2461 tacactcaat cgtaacggta atgggagacg acaattttat tacatggtta ttgtctggta
     2521 taattgtaaa gataaacgtg tcagtagtat tatcggcgaa actattatat tcttgaatat
     2581 ctatagatgg ctcattattg taaaaccata tttttttcta tctgagaact atattcattt
     2641 accttccagc gaccatgttg caggaaatag atgatatgca ggcctaaatc gttatctatt
     2701 tgcgaatgtt tatccagctt atcaatttca actatctttg aacgatgttt tttatcaacc
     2761 ttgccatggt gttgatgatt gttaacttga tctgaatgct tatcttgatg gcttgaagag
     2821 atgtgcttaa atatattgtg aaatactttc ataaataacc cgcaatattt ataacttgtt
     2881 agtaacaaaa taaacgatac atgtctgaaa gacatattta cgacgataca aagtaataat
     2941 acttatatat ccgatgattt taagcaccga gactatccgg tcttttacta aataatggaa
     3001 aacaatccat ccctggaaga taattgaaaa aaacaacaga ataagaatta atcttaggat
     3061 aaacatttat ttatcatggc cttttgaacg ctgaaaagta gcgctctgta aagtaaaccc
     3121 cgctatagtt atgctatgca tttgtcttta atgatatctc gtcattgtat taacaggctg
     3181 aaattatcat taatgcacat ttcaatatta gcaggga
//
LOCUS       DAJVOY010000272         3193 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVOY010000272 DAJVOY010000000
VERSION     DAJVOY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.275"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            102..3005
                     /locus_tag="OQL90_004011"
     rRNA            102..3005
                     /locus_tag="OQL90_004011"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3193
                     /gene="rrf"
                     /locus_tag="OQL90_004012"
     rRNA            3101..>3193
                     /gene="rrf"
                     /locus_tag="OQL90_004012"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          850 a          700 c          986 g          657 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgaatcgta agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccat tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatattg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgctgatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaactaa ggctgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatatgtagg tgaagtccct cgcggatgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggtcagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgttgacccg taatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgactccttg agagtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaaggtg ttttggcgga tgagagaaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc cat
//
LOCUS       DAJVOY010000273         3142 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVOY010000273 DAJVOY010000000
VERSION     DAJVOY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.276"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            315..474
                     /locus_tag="OQL90_004013"
                     /pseudo
     CDS             315..474
                     /locus_tag="OQL90_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(692..2782)
                     /gene="iha"
                     /locus_tag="OQL90_004014"
     CDS             complement(692..2782)
                     /gene="iha"
                     /locus_tag="OQL90_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCS1370593.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          786 a          829 c          727 g          800 t
ORIGIN      
        1 gcgagccgct tacgtgcagc agtcttatcc ggcagtttaa aggggaggac ggagaccaat
       61 gaggcactgg agcagattca gtgcagaggt gacctgcttt atcaaactta attgtgacaa
      121 aatggcgatt gagaaggtgc aattgactta tccggattcg gtcgtggcga ggtggtgccc
      181 ctgcattctg ctaaccgggt tatgagccag gagtcagcga tagacaaagg gacttatctg
      241 accgggcccc cggaaaaatg gcgggagaga atgatacctt cctgctgacg agaccggact
      301 ttgagatgac gagcgatagt gcggcagtga tgtacagcct gatcggtagc tgcaaaactt
      361 aacggaatcg agccggaaac gtggctacgc cacgtgatca gtgtaatcaa cacctggcct
      421 gccaaccgca tgaaagagtt gttgccctgg aacgtcactc tctctgtaaa ctaattttta
      481 ccccacgtcc ttcacggtgc gcttacactc tggacaagta tgtaaatgat aagttaaaaa
      541 taatccgtgc atttcctgtg acgatattag ttgttggtaa cgaaatctca ttagcggatc
      601 ggaaatgcac ttaacctaat acgttgccac tgttccgcca ggagttgcag tggcaacgta
      661 ttctaccgtc agtgatagcg ttttgttatt atcagaactg atagttcagc gacatccagt
      721 aatttcgctc aggtatcaca tatcctgttg ttgatgatcc cgtctggaag taatcaccgg
      781 catacagcgt actcttaccg gcactgtaca ggctcacgtc actgtaatcc ttgttgagca
      841 ggttattcac cgcagcattc agcgtcaggg catccgtcat cttccaggac agacctgcat
      901 ccaccaccgt ccaggctttc aggtattctc ctttctcatc atacactttc ttctgtacag
      961 cgctcagtga cgaataattc tgggtgaaac gtggtgtttt cccgcgataa cgggcaccca
     1021 gccatgatgc cacctcttcg gtgatctgcc agttcagttt cgcattcacc atgtgttcag
     1081 gggtataact cagcggcgca cctttgttat caccatcacg ttgttcactt cgggtccagg
     1141 tgtaattcag tgacagcgtg acatcctctg accacagcgg caatgtgccg gcaaattcca
     1201 caccgtgcaa ccgggccttt ccgctgttta catagctatt ggtgttatca tttatggaat
     1261 aagagacaat cttgttggag aagtcagtca taaaacctgt gacattggca ttcagaccgg
     1321 cggggttatc gtaatacacc ccagcctcat aactgacgct ctcttccggc ttcaggtcgg
     1381 ggttaccaag tagatttgtt tttccctgcc cggacacacc actaatccct ttatgtagct
     1441 gccccattct gggtgcctta tatcccgtgg tcacaccgcc tttcagcgtc caggcatctg
     1501 ccacatccca gaccagatat gcacgcggac tgaagtgtcc cccgaattgc tcatgatgtt
     1561 catagcggct gcccgcagtc agcgcaagtg catccgtgag atgccactca tcctcagcaa
     1621 ataccgacca gcttttctgc cggaaagttt cacctgtgct ggcaaggaca actccgtctt
     1681 tcatggacga gctctgaaac tcgcccccca ccgtaaccag atgagattct cccagagggg
     1741 taagcagtaa tgaattcagg ataaggttcg attccttaag ctcccgcggc tgaccggcaa
     1801 gcccccattt gtcgcgcttc agtacactgc gtacgagctc acgaccttta ttttctgtct
     1861 cgttccagtt cagatacgat ttccatgttc cgaaggtgaa agtatgatca tagccagctg
     1921 aaattttgtt ccgctcatag cgcagggtcc ggtcatatcc ccccgtcaga ctccccagtt
     1981 gcccatcccg gttatcataa cgctgccggg tggtatccat atcaaaccag agcacatcct
     2041 gctccgacgc cttccagtca agacgtgcac caagattata attctgtgac tccgtgggat
     2101 aaggaatacg cgtggctgct gtatcgctca gtgatgtgac cgatgagccc tgacgctgtt
     2161 gtgtgctacc gcgtacctgc aggctgacag aatcatccac aagggggcca ctgctccaga
     2221 aattaaactg gctgctgtta ccccatttgt tgctttcctg cagattcagc cctgcattga
     2281 cggaagagag ccatttgtct gcattctttc tggtaatgat attcaccaca ccgcccatcg
     2341 catcagagcc atacagtgtg gacatcggcc ccctgataac ctcaatacgc tcaatggcgg
     2401 ccagaggggg catgaacccg gtattcatgg cagaaaaacc gttgggagtc acgtcactgc
     2461 ttccgccctg acgaacacca tcaatcagta tcagcgtgta actggctggc attcctcgga
     2521 tgctgatttc cagcccaccg gttttacccg taccactttc aacatccaca ccctctactg
     2581 atctcagagc ctccgccaga tcgtggtact ggctggactg caattcctcc tggctaatca
     2641 cagaaacact ggcggctgcg ttagtcagct ttttctcata gccggatgcc gagacaatca
     2701 tcacatcctc tgcagcagct atgctgctgg ctgaaaatcc gagacaggga atgactacgg
     2761 aagccagagt ggttattcgc atattactaa tctccgatca tgttaaccgg caagacaata
     2821 cacaaataca aacaataatg agaataattt atatttgcct tttacaaaaa ataaagatta
     2881 ttaacaaaac taaatataaa taacaactgc ctcggcatgc cagttaacag ctatccgtat
     2941 ggcgtgttcg gttttgcgaa taaaaaatgg ttactgtaga agcggatttc caccacatgt
     3001 caaccatgaa tggtcagtgg tgcgagacga ctgggatgat ttggtcgatg acttcttccg
     3061 gtgaatactg atagaaacat tgaaatattg taagagaaag cgttcaaaat tctgtggcac
     3121 gtgaaaaatt ctacagcgtc at
//
LOCUS       DAJVOY010000274         3137 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVOY010000274 DAJVOY010000000
VERSION     DAJVOY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.277"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1639
                     /locus_tag="OQL90_004015"
     CDS             <1..1639
                     /locus_tag="OQL90_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCS1370594.1"
                     /translation="GMRLTWNKRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCA
                     LIPTTTPGVEIGRRHFPLNVPFQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLS
                     VGRGITLPSNSTGGVKSVALATGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDA
                     AASLITYGIMLGEKPAVLLAIVKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAY
                     QGAPIAITVEGANILTRSMMIFGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGH
                     VGSNKVRSFWLGLTRGLTSSTPTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKR
                     RERISARLGDILSQLYLASAVLKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQ
                     NFPNRVVAGLLNVVIFPTGRHYLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEH
                     NPVGLLEEALVDVIAADPIHQRICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILV
                     KAEESRLRSINVDDFDPEELATKPVKLPEKVRKVEAA"
     gene            complement(1682..2128)
                     /gene="ivy"
                     /locus_tag="OQL90_004016"
     CDS             complement(1682..2128)
                     /gene="ivy"
                     /locus_tag="OQL90_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1370595.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2309..3079
                     /gene="yafV"
                     /locus_tag="OQL90_004017"
     CDS             2309..3079
                     /gene="yafV"
                     /locus_tag="OQL90_004017"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1370596.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT          705 a          796 c          892 g          744 t
ORIGIN      
        1 ggggatgcgt ctgacttgga acaaacgcta tattacgctg gcaccgattg cgactgtgct
       61 tgggctggcg tttaaactct ccgacccgga aaagctgctg ggtggcgcag aagatttagg
      121 tattacctgt gcgctgatcc cgaccaccac gccgggcgtg gaaattggtc gtcgccactt
      181 cccgctgaac gtaccgttcc agaacggacc gacgcgcggt aaagatgtct tcgtaccgat
      241 cgattacatc atcggtgggc cgaaaatggc cgggcaaggc tggcggatgc tggtggagtg
      301 cctctcggta ggccgcggca tcaccctgcc ttccaactca accggcggcg tgaaatcggt
      361 agcgctggca acgggcgcgt atgctcacat tcgccgtcag ttcaaaatct ctatcggaaa
      421 gatggaaggg attgaagagc cgctggcgcg tattgccggt aatgcctacg tgatggatgc
      481 tgcggcatcg ctgattacct acggcattat gctcggcgaa aaacctgccg tgctgttggc
      541 tatcgttaag tatcactgta cccaccgcgg gcagcagtcg attattgatg cgatggatat
      601 cactggtggc aaaggcatta tgctcgggca aagcaacttc ctggcgcgtg cttaccaggg
      661 cgcaccgatt gccatcaccg ttgaaggggc taacattctg acccgcagca tgatgatctt
      721 cggacaagga gcgattcgtt gccatccgta cgtgctggaa gagatggaag cggcgaagaa
      781 caatgacgtc aacgcgttcg ataaactgtt gttcaaacat atcggtcacg tcggtagcaa
      841 caaagttcgc agcttctggc tgggcctgac gcgcggttta accagcagca cgccaaccgg
      901 cgatgccact aaacgctact atcagcacct gaaccgcctg agcgccaacc tcgccctgct
      961 ttctgatgtc tcgatggcag tgctgggcgg cagcctgaaa cgtcgcgagc gcatctcggc
     1021 ccgtctgggg gatattttaa gccagctcta cctcgcctct gccgtgctga agcgttatga
     1081 cgacgaaggc cgtaatgaag ccgacctgcc gctggtgcac tggggcgtac aagatgcgct
     1141 gtatcaggct gaacaggcga tggatgattt actgcaaaac ttcccgaacc gcgtggttgc
     1201 cgggctgctg aatgtggtga tcttcccgac cggacgtcat tatctggcac cttctgacaa
     1261 gctggatcat aaagtggcga agattttaca agtgccgaac gccacccgtt cccgcattgg
     1321 tcgcggtcag tacctgacgc cgagcgagca taatccggtt ggcttgctgg aagaggcgct
     1381 ggtggatgtg attgccgccg acccaattca tcagcggatc tgtaaagagc tgggtaaaaa
     1441 cctgccgttt acccgtctgg atgaactggc gcacaacgcg ctggcgaagg gcctgattga
     1501 taaagatgaa gccgctattc tggtgaaagc tgaagaaagc cgtctgcgca gtattaacgt
     1561 tgatgacttt gatccggaag agctggcgac gaagccggta aagttgccgg agaaagtgcg
     1621 gaaagttgaa gccgcgtaat tgaataacgg agccgaaagg ctccgtttct ttatccgcta
     1681 attatttaaa attaaagcca tccggatggt tttccaggct gccggtcaac gccgcgaaca
     1741 gcaccgtttt accatcaatc gaaagcgcat cgttcacatt cagccaggtg agtttctctt
     1801 gcgacgtttt ctcatcaata gtcgagaaca gccccgtcat ctgattagat ttctcggacc
     1861 acatcacagc gatacgttgc gagccacagt catgcggttt gcacgcgctc atcacctgat
     1921 acgtctcatc tcccaacgtt acggtttgtg cgggagtata agtaccgcct ttcatcaccc
     1981 aggcaggcag cttatgccct tgtaccatct gattaaatgc agctttggtg gtttcgccct
     2041 ttgcaaggct gctaatggtt aaatcatcct gcgccattgc actggtggcg atgaccagag
     2101 cggcgactgt cgttattgcc ttaaacatca ttcctcccga gcttatcctg cccatgttat
     2161 taaccttcta aagcctggcg ataacacgat aatttgcaaa tcagatattt ccaatatcag
     2221 catttgttat caatttattc tgcgctaagg caactgcctg ataaggtgtt aaagttataa
     2281 aacccttcgc ataaaggagt tgtacaccgt gcctggtttg aagattacgc ttttgcagca
     2341 accactggtg tggatggatg gccctgccaa cctgcgtcat tttgatcgtc aactggaagg
     2401 tattaccggg cgcgatgtga tcgttctacc ggagatgttt accagcggct ttgccatgga
     2461 agcggcagct tcgtcgctag cacaagatga cgtagtgaac tggatgacag ctaaggcgca
     2521 gcagtgcaat gcgctgatag caggcagtgt tgcattacaa acggagtctg gttcggttaa
     2581 ccgctttttg ctggttgagc cgggcggcac ggtacatttt tatgataagc gtcatctgtt
     2641 ccgcatggca gatgagcatc tacattataa agcgggcaat gcgcgagtga ttgtggaatg
     2701 gcgcggctgg cgtattttgc cgttggtgtg ctacgactta cgttttccgg tgtggtcgcg
     2761 caatctcaac gattatgacc tcgccatcta cgttgccaac tggcctgctc cgcgctctct
     2821 gcactggcag gcattgctga cggcccgcgc gattgagaat caggcgtatg tggcgggatg
     2881 caatcgcgtc ggcagcgatg gcaacggctg ccattatcgc ggtgacagcc gggtgattaa
     2941 tccacaaggc gagattatcg ctactgccga cgcgcatcag gcaacgcgca ttgatgcgga
     3001 gctgtcgatg atggcattgc gggaatatag agaaaagttt ccggcatggc aggatgcgga
     3061 tgagtttagg ttgcggtgag tgggggttgt gtttaaggct gggggagagt cggggtaaga
     3121 ctacgaaagc ccgctct
//
LOCUS       DAJVOY010000275         3134 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVOY010000275 DAJVOY010000000
VERSION     DAJVOY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.278"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..141
                     /locus_tag="OQL90_004018"
     CDS             <1..141
                     /locus_tag="OQL90_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="activator of the allDC-ylbA operon involved in
                     allantoin utilization; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCS1370597.1"
                     /translation="MRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNP
                     RS"
     gene            210..1304
                     /gene="mnmH"
                     /locus_tag="OQL90_004019"
     CDS             210..1304
                     /gene="mnmH"
                     /locus_tag="OQL90_004019"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1370598.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            1420..1785
                     /locus_tag="OQL90_004020"
                     /pseudo
     CDS             1420..1785
                     /locus_tag="OQL90_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2347..2796)
                     /locus_tag="OQL90_004021"
     CDS             complement(2347..2796)
                     /locus_tag="OQL90_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370599.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFSHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFLLVKLMLRIIL"
     gene            complement(2777..3134)
                     /locus_tag="OQL90_004022"
                     /pseudo
     CDS             complement(2777..3134)
                     /locus_tag="OQL90_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          839 a          723 c          711 g          861 t
ORIGIN      
        1 atgcgccctc cttcgccatt gagtctggca tggcgcaaat ttggcagcgg caaagcggta
       61 gaagatattg tgaccttgtt tacccagcgc aggccggaaa tcagcggatt tttagaaatt
      121 ttcggcaacc cacgcagtta aaaatcaagt ttccttgcgc gttggcgcgt aaaatagcgc
      181 gcaagctgat ttgaggttca cttttaacta tgcaagagag acacacggaa caggactatc
      241 gtgccctgct gattgctgat acgcccatta ttgatgttcg cgcccctatc gagtttgagc
      301 aaggcgcaat gcccgccgct atcaatctgc cgttaatgaa taacgatgaa cgcgccgccg
      361 ttggcacctg ctataaacag caagggtcag acgcggcgct ggcgctgggg cataaactgg
      421 tggcgggtga aattcgtcag cagcgcatag acgcctggcg ggcagcgtgc ctgcaaaatc
      481 cgcaaggtat tctctgctgt gcccgtggcg gtcaacgctc acatattgtg caaagctggt
      541 tgcatgcagc ggggattgat tatccgctgg tggaaggcgg ttataaggca ctgcgccaga
      601 ccgcgattca ggcgactatt gaactggcac aaaaaccgat agtgctgatt ggcggttgta
      661 ccggcagcgg taaaacgctg ttagtgcagc aacagccgaa cggtgtcgat ctggaagggc
      721 tggcgcgtca tcgcggttcg gcgtttggtc gcacgttaca accacaactt agccaggcga
      781 gttttgaaaa tctgctggct gccgaaatgc taaaaaccga cgcccgtcag gatttgcgcc
      841 tgtgggtgct ggaagatgaa agccggatga tcggttcgaa tcacctgccg gaatgtctgc
      901 gcgagcgaat gactcaggcg gcgattgcgg tggtagaaga tccgtttgag atccgtcttg
      961 aacgcctgaa cgaagagtat ttcttgcgta tgcatcatga ttttacccac gcgtacggcg
     1021 acgaacaggg ctggcaggaa tattgcgaat acctgcatca cggactttcg gctattaagc
     1081 gtcggctggg gttacagcgc tataacgaac tcgctgccca gctggatacg gcgctgacaa
     1141 cgcaactcac caccggcagc accgacggtc atctggcctg gctggtgccg ttacttaaag
     1201 aatattacga cccaatgtat cgctatcagc ttgagaaaaa agcggaaaaa gttgtctttc
     1261 gcggtgagtg ggcggaagtg gcggaatggg ttaaggcgcg gtaatgaggg acgtatttgt
     1321 attagtcgcg acttgatctg acacatggcc ttgaaaggtt gagagttacc ggttttgata
     1381 tgagtgtcga atccttatcc aaaacaagag gtaactctca tgcttcatac tgccaatccc
     1441 gtcatcaaac acaaagccgg tttgctcaat ctggctgaag aactcagcaa cgtatcaaaa
     1501 gcctgtaaaa tcatgggcgt ttcgcgtgat acgttttatc gttaccgcga actggtcgct
     1561 gaaggcggtg tggatgcgca gattaatcgt agtcgccgcg ctcctaacct taagaaccgt
     1621 accgatgagg caactgaaca ggctgttgtt gattacgccg tcgctttccc gacacatggt
     1681 cagcaccggg ccagcaacga gctgcgtaaa caggggggtt ttatctccga cagtggtgtc
     1741 cgttctgtct ggttactcca taaccttgag aatctcaaaa gacgatactg aacaaatttt
     1801 atcaggtgac gttccgtaaa aagttgtatg gagttctcga tgcagtcggc gcgactatca
     1861 gtacccccgc tgaaccctgg actatcgggc cgccgtactg gggcatgtca ccctggcgtg
     1921 ccgctggttt tccgctaatg gtttatttcc tctttctttc ccggcaagaa tttattttaa
     1981 tccggaagga ttatttattt tcagataggg actcacatta aacacgtaac attttaatta
     2041 atgcatggct gaatatgtat tacacaatgt cagatatcgt aaaagaccag tgcaattcta
     2101 tgtaaactct ctgactgttc attttatttg ttgtttcagg gtcggaactc accctcaata
     2161 aacaactgaa tacgagtaga tattcatcgt ctgagctata tggctttaca caatagccga
     2221 catgtctttt ctcagtcagg aattttactt ttcctcacta cgaagttgac gacttcgata
     2281 tgggatagac tcttaattca agcaattact ctgcacaagc atcaggcaat ccagctccaa
     2341 tatttgttat aaaataatgc gtaacattaa tttcaccaac aaaaaaaggt ttacaccatc
     2401 cccttgccct ggtatcttac catatttaca ccacactttt tttgtatctg gatcattctt
     2461 tgatacgatt ttcgccactt gttccctggc cctcactgtc aatgttttct taaacctacc
     2521 cccatcatca tttgttaggg aaaaataaga aaaaaaataa tcatcttcta catttgtgta
     2581 tattgatata tccaccgcta cgtgcgaaaa aaaatccggg agccatgtaa aaactccatc
     2641 attttctggt attgccatat agtttatgta atgtttactt acaagagatt catctttgta
     2701 taaataaaga taagtaatta cgcataaaac aacaatgatt ataatagcaa aaataaatat
     2761 tatcatcttt gatagattac ttgagatagc cagcatcttg taaagccttt atcgtttttt
     2821 tatgctctgg attaatataa tcactacatc tatctgagca atctgttgtt gatggacatg
     2881 tcaacccatg gtcatttaca gccaagtctt ttttattatc ttcaatcatt tcagaatggg
     2941 atagctttac ttttctgccg tacataccga acagatttaa tccgtaatct ctaatctctt
     3001 tttcataacc cagccctttc gccgatcggc taacaccagc gtcatttaat ttagacactc
     3061 gacaaaatgc catacaatgg aaaaattgat cagtcccacc taaatttaat ctcttcataa
     3121 gtatcagata atat
//
LOCUS       DAJVOY010000276         3077 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVOY010000276 DAJVOY010000000
VERSION     DAJVOY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.279"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..913)
                     /gene="curA"
                     /locus_tag="OQL90_004023"
     CDS             complement(<1..913)
                     /gene="curA"
                     /locus_tag="OQL90_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1370600.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVK"
     gene            1094..1612
                     /gene="mddA"
                     /locus_tag="OQL90_004024"
     CDS             1094..1612
                     /gene="mddA"
                     /locus_tag="OQL90_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS1370601.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1609..2058
                     /locus_tag="OQL90_004025"
     CDS             1609..2058
                     /locus_tag="OQL90_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1370602.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2059..2292)
                     /locus_tag="OQL90_004026"
     CDS             complement(2059..2292)
                     /locus_tag="OQL90_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCS1370603.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2378..2551)
                     /gene="ortT"
                     /locus_tag="OQL90_004027"
     CDS             complement(2378..2551)
                     /gene="ortT"
                     /locus_tag="OQL90_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS1370604.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2746..2841
                     /gene="yncL"
                     /locus_tag="OQL90_004028"
     CDS             2746..2841
                     /gene="yncL"
                     /locus_tag="OQL90_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS1370605.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          747 a          788 c          720 g          822 t
ORIGIN      
        1 ctttcaccca ttgccccatc tccttctgaa actcatggat gcggtgacca taatcctgag
       61 cgataataaa cccttgcaag cgaatacgtt ttttcagcac cgttgccatc aacaggggta
      121 aacgatccgg accgggtggt agctctgtag cgttatagct gctcactaag ccacaaacgg
      181 gaatgcgcgc agatgtatta agtaatggta gcactgcatc aaatacctta ccgcccacgt
      241 tttcataata gatatcaata ccttttgggc acgctttcac cagttgttcg gcaaaatcac
      301 ccgcgtggtg atcaagacaa acatcgaagc ccagcacctc gatagcatgg cggcattttt
      361 ccgcgccacc ggcccccccc accaccctgc aacctttaag tttgccgatt tgccccaccg
      421 ttgctcccac aggacctgtc gccgcagcta ccaccaacgt ttcgccctct ttaggctgac
      481 cgatatccag cagccccata taagcggtaa agcctggcat ccccagcaca cccagcgacc
      541 acgatggatt ttgcggatga tcgccaagtt tcaccagatc atcaccactg gatatgtcat
      601 agtcctgcca tccactgtag cccagcaccc agtcgccagg ctgataatca ggatgattcg
      661 actccacaac atggctcacc gtgccgccga ccatcacgcc gccaatatca acaggcggtg
      721 aataagatgg ctcatcgctc atacgtccac gcatatacgg gtctagggac aaataaactg
      781 tgcgcagtaa cacctgtcct tcacccggtg tggcgacatc atcttcttca agacggaaat
      841 tctccggaac cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga ttacgctgct
      901 tttgttgccc cataatgctc tcctgtgctt ttggcatgaa tccatcaata gtagcaggct
      961 cacgggattg gttttgtttg cagccgtcac atcccctctg caacacgcaa taatttcatc
     1021 gaggaatccg ttgcttcggg ctgttttcga tagtattgat aacatcatca tcattatttt
     1081 atcgaggcca gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga
     1141 aatttataac cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa cggttgatgc
     1201 agataaccgc attgcctggt ttgaagcgcg gaccatagca ggttatccgg tgctggtaag
     1261 tgaagaagat ggcgtagtga cgggatatgc ctcgtttggc gactggcgta gtttcgatgg
     1321 ttttcgccat accgtggaac attcggttta tgtccatccc gatcatcagg gcaaaggtct
     1381 ggggcgtaaa ttgttaagcc gattgattga tgaagcgcgg gatagcggga agcatgtcat
     1441 ggtcgccggg atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt
     1501 tgtcgtcacc gcacaaatgc cgcaggtggg cactaaattt gggcgctggc tggatctgac
     1561 atttatgcag ttgcaactcg acgaacgcac tgaaccggac gcgattggat gaatcagtcg
     1621 ctcacccttg cttttttgat tgccgccgga attggtctgg tagtccagaa cacgctgatg
     1681 gtgcgcatca cccagacctc ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg
     1741 gggattgtgc tctttgtttc tattttatgg tttaaacaag gcatggcggg gtttggcgaa
     1801 ctggtgtcca gcgtgcgctg gtggacactt atacccggct tgctgggatc gttttttgtc
     1861 ttcgccagta tcagcggata tcagaatgtg ggagcggcga caactattgc agtactggtc
     1921 gccagccagc taattggcgg tctgatgctg gatatcttcc gcagtcatgg cgtaccacta
     1981 cgggcgttgt tcggcccaat ttgtggcgca attttacttg tggttggcgc ctggttggta
     2041 gccagacgct cattttaatc agaggatggt gccgcctttg gttaactgtt cgtggcgcgc
     2101 ttccatatct tctttatgct tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg
     2161 ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga ttaataattc
     2221 gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc
     2281 cagatgcgac atactttctc cttaaccact tgtgaaattt atcgattcat ccaaatgata
     2341 taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa aacaacagcg
     2401 ccaccgggaa actcatcggc catgttgccc ccaccagaaa ggcgcttaag aaacggatgc
     2461 gtttcttatc gtgagaaaga aaccaggtga taagaaatgc gattgctgcc ataaccgcat
     2521 aaaaaacaag catatgttga tagagagaca tgtacgggcg tccagccaga tgaataaaac
     2581 gcgcgcaaat atgcgtcatt ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa
     2641 atcatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg cggattttct
     2701 taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt
     2761 agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct tatctctatg
     2821 agatttggct ggtttatttg atgtagtgat tttgcacgca gcacgtactg tcagagaaat
     2881 caactctctg acagtacgca agatcttctc atactctcca ggtttacaac attatcctta
     2941 atatttactt ctttttgctg gcatctacat tgaaatgagt agaaaacatc cccctactgt
     3001 cgtctataca aacagcttac tgattcaaac ggaatcttgt tttcacattt ttctatcatg
     3061 aatactttat acgcgcg
//
LOCUS       DAJVOY010000277         3067 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVOY010000277 DAJVOY010000000
VERSION     DAJVOY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.280"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(51..235)
                     /locus_tag="OQL90_004029"
                     /pseudo
     CDS             complement(51..235)
                     /locus_tag="OQL90_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(397..471)
                     /locus_tag="OQL90_004030"
     tRNA            complement(397..471)
                     /locus_tag="OQL90_004030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(436..438),aa:Arg,seq:cct)
     gene            complement(547..1479)
                     /locus_tag="OQL90_004031"
     CDS             complement(547..1479)
                     /locus_tag="OQL90_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1370606.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1773..2528
                     /gene="mlaA"
                     /locus_tag="OQL90_004032"
     CDS             1773..2528
                     /gene="mlaA"
                     /locus_tag="OQL90_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1370607.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2710..3045)
                     /locus_tag="OQL90_004033"
     CDS             complement(2710..3045)
                     /locus_tag="OQL90_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370608.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLS
                     APGVNFDDI"
BASE COUNT          767 a          709 c          711 g          880 t
ORIGIN      
        1 cccatctttt acggggtgaa ggtagatttc gtatgaaggg tatctgttgt ctcctgcaaa
       61 gtcataagcg gatattttcc tacggtcagt attttttcct taccgtcaat cttgtagcga
      121 agttgccata ccttttcccg gatacaggaa cataaaggta caggccatta ccatcgagaa
      181 ggcggtatgg tttttctttc ggctttgctg cttcaatctg cttaacggtg agcatgggta
      241 aaaatccggt gggtaaaatt attttatcca ctttttaccc gtcatggagt gcggctgtca
      301 acgatctgac gcgaaccatg acgaaccgtg aatatacgga aggcttgata ttcaggggat
      361 tttgcggact ggtacggatg ggagcgaact gataaatggt gtcccctgca ggaatcgaac
      421 ctgcaattag cccttaggag gggctcgtta tatccattta actaagagga caatgcggca
      481 tgagtatacc cgctaatgga ctgcggagta agtacactgc cgctcgattg cttaaaccct
      541 cgccatttat gctgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg
      601 gcgtgcttct gctttgcgct tgttgctcat gtcgttacgg atctgtgcat gactcattaa
      661 cgcgaagata aaggtgccgc cgcagatatt ccccgctaaa gtaggtagtg cgaagggcca
      721 aatgaaatcg ctccagtgca gcgtgccgtt aaacaccaga tagaggattt ctacagaacc
      781 gacaacgatg tgggtggtgt cacccagtgc aataagccag gtcatcaata taatcaccac
      841 aatctttgcc gcacccgctg caggaaacat ccaaaccata gtggcgatca gccagccgga
      901 aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt
      961 gacaaatgca tcgtgagttt cttcattgaa gataggcata tattcaaatg cccatgcagc
     1021 aatacctgtc ccgagaatat tacccagcag cacgacgccc cataatcgca taagtaagcc
     1081 gacgttgctc attgtcggtt tttgcatgac gggtagtacc gcagtcacgg tgttttcggt
     1141 aaataattgc tggcgggcca taatgacgat aataaaacca aaggtataac cgagattctc
     1201 caacaagaag ctgcccggca cgccttccag ttcgacatga aatatccctt ttgccagtaa
     1261 cgaagcgccc atcgacagac ccgccgcaat ggctgaccac agtagtgcca ttgcgtcgcg
     1321 ttccagctct ttttcaccat cctggcggat atgttcatga attgccatcg cccgggaggg
     1381 gagtcgatct tcatctattt ctattttttt gccgcgctct ttttcttcgc tctcaacttc
     1441 aatttcgtcg ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc
     1501 acgtatagct aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat
     1561 gtagaaaagg ccgcgtttgc ctttttctgt ttctatagaa tcaagtagcc tacagggcgg
     1621 cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct
     1681 gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac
     1741 tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc
     1801 tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg
     1861 acccgttaga agggttcaac cgcaccatgt acaacttcaa cttcaatgta ttagacccgt
     1921 atattgttcg accggtcgct gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaacg
     1981 gtttgagcaa ctttaccggc aaccttgaag aacctgcggt gatggttaac tacttcttgc
     2041 agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac accattttgg
     2101 ggatgggcgg ctttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac
     2161 ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatgggcct tacgttcagt
     2221 taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggca gatggtcttt
     2281 acccggttct ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggga
     2341 tcgaaacccg cgctcagctg ctggattccg atggtctgct gcgtcagtcg tccgatcctt
     2401 atattatggt gcgcgaagcg tacttccagc gtcatgattt catcgctaat ggcggcgaac
     2461 tcaaaccgca ggaaaatccg aacgcacaag cgattcagga tgatttaaaa gatattgatt
     2521 ctgaataaga aacaaataaa aaaggtgagt cgcaaaactc accttttttg ttgttatccc
     2581 tcaattacca caagtaaaag gagataaacc gacaatgacc cggacgtaat gatgtaaaaa
     2641 tactgatgtt cgtcactgac tatttccgat attcaggcgt tgaaaaatca aaaaactgga
     2701 aaaataattt taaatatcat caaaattaac acctggcgca gatagttgat taatttcatt
     2761 ttgatattcc gtctggcgat tttgaagcct tttttcaact ttatcttcga gatccttaaa
     2821 gtctttgtat ttattttcag gatggtcaac gtcaactttg ttagcgaata aagtagaaat
     2881 tatttctttt acattatcgc gtctaaattt tgcatcgtcc acttgaaaat tatatcttat
     2941 tgctcgacct aaacgtttaa aaacttcgtt aggttctctc agtctttccc cgttgagctc
     3001 gtttacaacc tgatgatcgg aacctgttgc aaatgattct gccatgatag ctttactgct
     3061 ttcaaac
//
LOCUS       DAJVOY010000278         3018 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVOY010000278 DAJVOY010000000
VERSION     DAJVOY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.281"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..295)
                     /gene="fliE"
                     /locus_tag="OQL90_004034"
     CDS             complement(<1..295)
                     /gene="fliE"
                     /locus_tag="OQL90_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1370609.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQE"
     gene            510..2168
                     /gene="fliF"
                     /locus_tag="OQL90_004035"
     CDS             510..2168
                     /gene="fliF"
                     /locus_tag="OQL90_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCS1370610.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2161..>3018
                     /gene="fliG"
                     /locus_tag="OQL90_004036"
     CDS             2161..>3018
                     /gene="fliG"
                     /locus_tag="OQL90_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1370611.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     VATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADIL"
BASE COUNT          748 a          799 c          840 g          631 t
ORIGIN      
        1 cttcctgata cgccgccacc agcttattac gcacctgaat ccccatttgc atagaaactg
       61 aggctttttg catatcggtc atcacatcgt ttaacgccac gccgggttca ccgagggtga
      121 atttttctgc ctgcgtgcgg gcggctgttt gtgtatcgct tatgcgatcg agcgcggcgt
      181 gcagctgccc ggcaaaacta atggtcggtt gcggcagtga atcctgcgca cgcgcactca
      241 tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc ctgtatcgct gacattttcg
      301 tctcctggat aatttctggt agcaaagcct accagtaagt caataagaca aaggcgctaa
      361 atagcaacaa aaaaacgggt ttattggcgg atagaaaaaa acgaaagcac aaataatggc
      421 agcgtcaatt tttcgagttt gctgacccgg gagtgagtct tgttccactt tgccaataac
      481 gccgtccata atcagccacg aggtgcgcga tgaatgcgac tgcagcccag acaaaatctc
      541 ttgagtggct taatcgcctg cgtgcgaatc cgaaaattcc attgattgtt gccggttccg
      601 cggcagtggc ggtcatggtc gcactgatcc tgtgggcgaa agcccccgac taccgcacat
      661 tattcagcaa tctttccgat caggatggtg gcgcaattgt cagccaactg acgcaaatga
      721 atattcctta ccgcttcagc gaagccagcg gcgctattga agttccggca gataaagttc
      781 acgagctgcg tctgcgcctg gcgcaacaag gtttgcccaa aggcggcgcg gtcggtttcg
      841 aactactaga tcaggaaaag tttggtatca gccagttcag cgaacaggtg aattatcagc
      901 gggcgctgga aggcgagctt tctcgtacca tcgaaactat cggcccggta aaaggggcgc
      961 gcgtacatct ggcaatgccg aaaccgtctt tattcgtccg tgaacaaaaa tccccttctg
     1021 catcggtgac ggtaaatcta ttacccggcc gcgcactcga tgaagggcaa attagcgcca
     1081 ttgtacatct ggtttccagc gcggttgctg gtctgccgcc ggggaacgtc acgctggtgg
     1141 atcagggtgg acatctgtta accctgtcca ataccagcgg gcgcgatctt aatgacgctc
     1201 agttgaaata tgccagcgat gtcgaaggcc ggattcagcg gcgtattgaa gcgatcctgt
     1261 cgccaattgt tggtaacggt aatattcacg cccaggtcac ggcgcaactg gacttcgcca
     1321 gtaaagaaca aacggaagaa cagtatcgcc ctaacggtga tgaatctcat gcggcgcttc
     1381 gttcacgcca gcttaatgag agcgagcaaa gcggttccgg ttatccgggc ggcgtaccgg
     1441 gggcgttgtc gaatcaaccg gcacctgcga ataacgcgcc aatcagcacg cctccggcaa
     1501 atcaaaataa ccgccagcag caggcgagca ccaccagcaa tagcgggccg cgtagcacac
     1561 agcggaatga aaccagtaac tacgaagtcg atcgcaccat tcgtcatacc aaaatgaacg
     1621 tgggcgatgt gcaacgtctg tcagtcgcgg tagtggtaaa ttacaaaacc ttgccagacg
     1681 gtaaaccatt gcctctcagc aacgaacaga tgaagcaaat tgaagatctg acccgcgagg
     1741 cgatgggctt ttctgaaaaa cgcggtgact cgctcaatgt cgttaactcg ccgttcaata
     1801 gcagtgacga aagcggcgga gaactgccgt tctggcaaca gcaagcgttt atcgatcagt
     1861 tgctggctgc cggtcgctgg ttgctggtac tgctggtggc gtggctgctg tggcggaaag
     1921 cggtacgccc gcagttaaca agtcgcgccg aggcgatgaa agctgtacag caacaggcgc
     1981 aggcccgtga ggaagtggaa gatgcggtgg aagtccgcct gagcaaagac gaacaactcc
     2041 aacaacgacg cgctaaccaa cgtctggggg cagaagtcat gagccagcgt atccgtgaaa
     2101 tgtctgataa cgatccgcgc gtggtggcgc tggtcattcg ccagtggata aataacgatc
     2161 atgagtaacc tgacaggcac cgataaaagc gtcatcctgc tgatgaccat tggcgaagac
     2221 cgggcggcag aggtgttcaa gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca
     2281 atggcgaacg tcacgcagat ctccaacaag cagctaaccg atgtactggc ggagtttgag
     2341 caagaagctg aacagtttgc cgcactgaat atcaacgcca acgattatct gcgttcggta
     2401 ttggtcaaag ctctgggtga ggaacgtgcc gccagcctgc tggaagatat tctcgaaact
     2461 cgcgataccg ccagcggtat tgaaacgctc aactttatgg agccgcagag tgccgccgat
     2521 ctgattcgcg atgagcatcc gcaaattatc gccaccattc tggtgcatct gaagcgcgcc
     2581 caggccgccg atattctggc gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt
     2641 gtcgccacct ttggcggcgt gcagccagcc gcgctggcgg aactgaccga agtactgaat
     2701 ggcttgctcg acggtcagaa tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc
     2761 gaaattatca acctgatgaa aactcagcag gaagaagccg ttattaccgc cgtgcgcgaa
     2821 ttcgacggcg agctggcgca aaaaattatc gacgagatgt tcctgttcga aaatctggtg
     2881 gatgtcgacg atcgcagcat tcagcgtctg ttgcaggaag tggattccga atcgctgttg
     2941 atcgcgctga aaggggccga gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag
     3001 cgtgccgccg atattctg
//
LOCUS       DAJVOY010000279         3012 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVOY010000279 DAJVOY010000000
VERSION     DAJVOY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.282"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            198..938
                     /locus_tag="OQL90_004037"
     CDS             198..938
                     /locus_tag="OQL90_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1370612.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(909..1676)
                     /locus_tag="OQL90_004038"
     CDS             complement(909..1676)
                     /locus_tag="OQL90_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1370613.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1882..2460)
                     /gene="lpcA"
                     /locus_tag="OQL90_004039"
     CDS             complement(1882..2460)
                     /gene="lpcA"
                     /locus_tag="OQL90_004039"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1370614.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2700..>3012
                     /gene="fadE"
                     /locus_tag="OQL90_004040"
     CDS             2700..>3012
                     /gene="fadE"
                     /locus_tag="OQL90_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCS1370615.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQ"
BASE COUNT          722 a          759 c          765 g          766 t
ORIGIN      
        1 tcttaggaaa taaatgatac atgcgctttt cggactattg aaaactgccc ctgtgctaaa
       61 tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt tggtgataat gtgagccttc
      121 caaatccaga ctattccgat ttgggatcca ttttgccgtc ctggcgtgtg taaccgtttt
      181 atcaaggaat aagcagtatg cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt
      241 gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac cacgccagtg agcaaagagt
      301 ataagcagca gttgatggga tcccctgtct acatccagat cttcaaggaa gaacgtacgc
      361 tcgatctcta cgtcaaaatg ggcgagcaat atcagctgct cgacagttat aaaatctgta
      421 aatattccgg cggcttaggg ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt
      481 tttatagcgt ccagcgtaat cagttaaaac cagacagccg ttactacaaa gcgattaata
      541 ttggtttccc caatgcctat gaccgtgcgc atggttatga agggaaatac ctgatgatcc
      601 acggcgattg tgtttccatc ggctgctacg caatgaccaa tcagggtatt gatgagatct
      661 tccagttcgt tactggtgcg ctggtgtttg gtcagccgag cgtgcaagtg agtatttacc
      721 cgttccgcat gaccgacgcc aatatgaagc gccataaata ttccaacttt aaggacttct
      781 gggagcaact gaagccgggt tacgactact ttgagcaaac ccgtaagcca ccgaccgttt
      841 ctgtggtcaa tggtcgttac gtggtcagca agccgttaag ccacgaagtt gtgcagccac
      901 aactggcatc aaactacacg ctccccgagg caaaataagc gccattcgcc tggcataatc
      961 ttttgccagg tttcattgcc cgtcagcggc tgtgtcgcaa tcaccgtgac cacatcattc
     1021 ggtgtggtct gcgagctgaa gtcgatttct acatcctgat ccagcagcgt tgccacgcca
     1081 aacggtgcgc ggcgggtgat ccagtgcaga ttcgtcgagc aatacgccat tacatagcgc
     1141 ccatccgaaa gcagcatgtt gaaaacgccc ttctgccgca gttcgtccgc cagtgaagcg
     1201 atatatttaa acactgctgc catgttgccc ggcgtgcgcg ggtaacgctg cgttaattta
     1261 tgcagcagcc agcagaaggc tttttcgctg tcggtttcgc ctaccgggcg gaagttgccg
     1321 gtttccagtg atttgtagcc cgtcagttgt ccgttatggg cataagtcca gttacgcccc
     1381 cataactcgc gggtaaatgg gtgagtattt tccaacgcca cctcgccccg attagcctgg
     1441 cgaatatgag ccaccaccga acaggatttt atcgggtagt cctggacaag tttggcgatg
     1501 ggggaattaa agctgggttg tggatcttta aatgtgcgac agcctttacc ttcgtaaaag
     1561 gtaatgcccc agccatcttt atgtggcccg gttccaccac cacgctgtac aagtccggtg
     1621 aaactaaagc agatatcggt agggacgttg gcgctcatcc cgagcaattc gcacatgggt
     1681 aacctccaca acagcgggag cgcatggccc ccggcgaaga ttgatttaag aattttcagg
     1741 tcggatgcgg cgtaaacgaa ttattcggcc tacggttcgc gtacagattt gtaggcatga
     1801 taagacgcgt cagcatcgca tcaggcatca gcgcacaagt gccggatgcg gcgtaaacgc
     1861 cttatccggc ctacgccaga cttacttaac catctctttt tcaattaact ggatcaggat
     1921 atggatcact ttaatgtgaa tctcctgaat gcggtcggca taaccaaagt gcggtacgcg
     1981 aatttcgata tccgccgtgc cagccatttt gccgccgtct ttaccggtca gggtgatcac
     2041 tttcatcccc ttctcacgcg ccgctgcgat cgctttgatc acgtttgcag agttaccgga
     2101 ggtggagatc cccagcagta catcgccttc gcgacctacc gcttcaacgt agcgggagaa
     2161 aatatcattg aaaccgaaat cattaccgac gcaggaaata tgactaacgt cagaaatagc
     2221 aatcgccggg tagcccggac ggttttcacg gtagcgaccg gtcaactctt cggcaaagtg
     2281 catagcgtcg caatgggaac cgccgttgcc gcaggaaagc actttgccac cggctttaaa
     2341 gctgtctgct aacaggaccg ccgcgcgctg aatggcgtga atattggcgt catcttttaa
     2401 aaagttagcc agcgtttccg ccgcttcgtt cagttcgtta cgaataagat cttggtacat
     2461 gaggatatcc ttcagcataa atgtaataga caaaatgcag tgtaccggat accgccaaaa
     2521 gcgagaagta cgggcaggtg ctatgaccag gactttttga cctgaagtgc ggataaaaac
     2581 agcaacaatg tgagctttgt tgtaattata ttgtaaatac attgctaaat gtatttacat
     2641 ccactacaac catatcatca caagtggtca gacctcctac aagtaagggg cttttcgtta
     2701 tgatgatttt gagtattctc gctacggttg tcctgctcgg cgcgttgttc tatcaccgcg
     2761 tgagcttatt tatcagcagt ctgattttgc tcgcctggac agccgccctc ggcgttgctg
     2821 gtctgtggtc ggcgtgggta ctggtgcctc tggccattat cctcgtgcca tttaactttg
     2881 cgcctatgcg taagtcgatg atttccgcgc cggtatttcg aggtttccgt aaggtgatgc
     2941 cgccgatgtc gcgcactgag aaagaagcga ttgatgcggg caccacctgg tgggagggcg
     3001 acttgttcca gg
//
LOCUS       DAJVOY010000280         3006 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVOY010000280 DAJVOY010000000
VERSION     DAJVOY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.283"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..319)
                     /locus_tag="OQL90_004041"
     CDS             complement(<1..319)
                     /locus_tag="OQL90_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370616.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSG"
     gene            complement(385..729)
                     /locus_tag="OQL90_004042"
     CDS             complement(385..729)
                     /locus_tag="OQL90_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCS1370617.1"
                     /translation="MTEADLYPHLAHLAGGQVSPYVAPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(726..1172)
                     /locus_tag="OQL90_004043"
     CDS             complement(726..1172)
                     /locus_tag="OQL90_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCS1370618.1"
                     /translation="MIEILLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRTPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(1169..1489)
                     /locus_tag="OQL90_004044"
     CDS             complement(1169..1489)
                     /locus_tag="OQL90_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1370619.1"
                     /translation="MISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGRSSRERMQSGA
                     EMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRL
                     GGEK"
     gene            complement(1529..1855)
                     /locus_tag="OQL90_004045"
     CDS             complement(1529..1855)
                     /locus_tag="OQL90_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCS1370620.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVLREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1852..2451)
                     /locus_tag="OQL90_004046"
     CDS             complement(1852..2451)
                     /locus_tag="OQL90_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCS1370621.1"
                     /translation="MPAGKSLTPDDVRPMLEQMVTEAVSHIPVPRDGRDYDPDVLQKA
                     VLDAVSALPAPQDGRDATALEILPAIDVQKSFPRGTYATHQGGLWRAYEKTHGMRGWE
                     CLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTV
                     TWGGSLWHCNSMTEDKPREAHSSAWTLAAKRGRDAGGGK"
     gene            complement(2442..3006)
                     /locus_tag="OQL90_004047"
                     /pseudo
     CDS             complement(2442..3006)
                     /locus_tag="OQL90_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
BASE COUNT          650 a          946 c          749 g          661 t
ORIGIN      
        1 taccggacgg aaacagcacc ttaaacgcat acaacgcgtc attgtcatag gcatcacgca
       61 gggcgttctg ggcctgattc agataaaaat tacccgacat ggaaatctca gacgacgccc
      121 ccagaccgtt gatgttctcc tgctctgtgg agcagagcgt ggtcacatca atatcctgtt
      181 tctgaccggc ggtgaactgg acttccttga tggtgcagtc caggcgcaga tattccgcct
      241 tatccatagt ttcagcagtc gccggggcag atgaaatcat cacctgcgtc agctgtgagc
      301 gttcatacaa agcagacatt ctgcctcctg ataataaaaa acccgcacgc ggcggggtat
      361 ggttttgtag aaaaaaagaa aaagtcacac cgtgacctga aactccaggg ttgcacggta
      421 acagcggttt tccggaatat agtcctgcat ttcactgacg gatcccgggg ccagcagcat
      481 tatggcttca cgggcgtcct gacgtatctg acgcgcctgc gtcacagtcc cggcataaac
      541 gtctatctgc accgacactg aggactccgc ctgcccgccc atcacgtccg ctgacaccga
      601 tgaaatcagg ctgaaaacca cccacggaag cgccaccgac ggcctgccat ccagcagggg
      661 ggccacatac ggggacacct gcccgccggc aagatgcgcc agatgaggat acaaatccgc
      721 ctccgtcatc gtctcagtac ctcatcaatg gcccggttca tccgcgcaat cgccacctga
      781 gctgcctgtt cactgcgcac atcaaacgcc gggcgcacaa acgggtgcgg tggcatattc
      841 acggtcccca tttccacaaa ccgccagtag aaagcattgc gcgggttatc cgccttcatg
      901 gtgttatcgc tgttaccggt gtccggatta acaccacgga tatggacacc ggattccatc
      961 ccgccatcgc gggagcgccg ggaaaggatc accacattgc ggcgcagttt tcccctgcgt
     1021 accggtgtcc gtgacaccac ttcttctttc agcacattcg cacccgcacg ggttgcctca
     1081 cgcagcaccc ggttattttc cgcaccactc agaagctgca aatcgcggct gatgtcctcc
     1141 agccccgaaa aatccagcag gatttcgatc atttttcccc tcccagccga cagagaattt
     1201 ccagacgccc gccggtcgca tccggcacgg gcagcccgac aacgttcagg atccggtcac
     1261 gccatggacc actcagcaca tgaagtcgtg acgctgccgt gatttcccga ccggactgac
     1321 cgcgcaccca gatgcggatt tccgcctgcg ccatttccgc accggactgc atccgctccc
     1381 ggctgctcct gcctcggata tccgcatgaa ttttcccgca tgacacccat tcttccgtca
     1441 tttctccggc agcgttacgg gttaacaccg ggttcagaac acttatcatc tatgtcagac
     1501 gacctgcaga tattgccatt ccccctcctc ataacaccgt cggacaacgc aaatcgtaaa
     1561 tcagcacgga aacagaaaac ggcagctccc cctgaagcag ttcttcccgc tccgcaagat
     1621 ccggattccg gtacagcatc ccggtcagtc gcatggcagc ccccttcatc cgggttaatg
     1681 cctcgcccgg gatcagttca ccgtcctcac ggagcacttt atcccggctg ccctgaatgt
     1741 aggccagcag cacggcggta gcctgacgaa ccttgtccat cagcatgtca tcatccgcgt
     1801 catggtcgac acgcagatgt gccttgatct cttccagtgt cagtaatgcc gtcattttcc
     1861 gcctcctgca tcccgcccac gttttgcagc cagggtccag gctgatgaat gagcttctcg
     1921 gggtttatct tcggtcatac tgttgcagtg ccacagcgag cccccccacg tcaccgtatc
     1981 gccggggtgg taggtttcac cggctctgaa cacaccgcgg tagagcatca ccggcaggga
     2041 aaatgttttt tccgtacgct ggccactgct ctgccggacc accacagaga acaaccgctc
     2101 acccgtcatg ctgacgtcaa tatccgccac cccgtcaacc aggcattccc atccccgcat
     2161 cccgtgcgtt ttttcatacg cccgccagag tccgccctgg tgtgtggcat acgtgccccg
     2221 gggaaaggat ttttgaacgt caatggcggg gagtatttcc agagccgtgg catcacgccc
     2281 gtcctgcgga gccggcaggg cactcaccgc atccagaacc gccttctgca gaacatccgg
     2341 atcgtagtca cgaccatcac gcggaacagg gatatggctt acagcctccg tcaccatctg
     2401 ttcaagcatc ggacgcacat catccggggt gagactttta ccggcaggga tgctttcacc
     2461 atatacgaac cgtttctttc gggcgtcatg gtgacctcgg ctacaccctg cgtattagta
     2521 gagaccgtat tctggctgat gatcatttgc gaggatggct ctgcactgaa tgtcacgggt
     2581 tgatgcgcaa ccgggttgcc aaactggtcg ttaacgtgag ctttaactgc tacgtcttgt
     2641 gcgaccatta cctgaccttc cggtatctcc agactggtaa tcgtcgcaga attaacatct
     2701 gcactggcat tcagtaatcg cgagatgact tcagtaggtt tatctgccag agaggctgaa
     2761 actgttttca gtccgacctc ggtgcttgtc acaagaattt cagcgaaacc ccgatcatcc
     2821 gtttcaacgc tggtgctgct tagcgtgacg gaggttccgc ggaaattaac ttttatgcct
     2881 tctaccgggt tgccgttagc gtcagtgacg cttgccgtca tggttgcgcc ctcggtcgtg
     2941 ctgacgacga atgagtatac agaggttaag gaggctagtt ttgcggtacc agcatcggca
     3001 atcaaa
//
LOCUS       DAJVOY010000281         2906 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVOY010000281 DAJVOY010000000
VERSION     DAJVOY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.284"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            91..2607
                     /locus_tag="OQL90_004048"
     CDS             91..2607
                     /locus_tag="OQL90_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1370622.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPRHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGLQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVIPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFNTPDSEPTTSVLQGTAQCH"
     gene            2618..>2906
                     /locus_tag="OQL90_004049"
     CDS             2618..>2906
                     /locus_tag="OQL90_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1370623.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTG"
BASE COUNT          823 a          706 c          674 g          703 t
ORIGIN      
        1 atgggctgca atcaatgatt atggcggcag ttccgggaca gaaactcgtc cactgcaata
       61 acaaatataa aaaacacagg tcatcaggga atgccacaac gacaccacca gggacataaa
      121 cgcacaccga aacagttggc gctcatcatc aaacgctgtt tgccgatggt gctcactggc
      181 agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc cattatgctg
      241 gaaactacaa aaagtggtat gcaaacaact gatctgtcac gtttttcaaa aaaatacgca
      301 caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt ttcacagaaa
      361 aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac ggtagaacaa
      421 ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa agatgacgat
      481 agcgtgatca actcgcttga acaaatcatt cccggtacag ctgctgaatt tgatttcaat
      541 catcagcgac ttaatttgag cattccccaa attgcactgt accgtgatgc aagaggttac
      601 gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta ctcgtttaca
      661 ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct gaatatgcaa
      721 aatggtgcta attttggccc ctggcgatta cgtaactatt ctacgtggac acgcaacgat
      781 caggcgtcaa gctggaacac tatcagtagt tatttacaac gtgatatcaa ggcgttgaag
      841 tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag ctacaccttt
      901 actggcgtgc aactcgcttc cgacgataat atgttgccaa acagccagcg cggatttgcc
      961 ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca aaatggttat
     1021 gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct ctacccctct
     1081 tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacaca acgtcgcttt
     1141 atccagcctt attcttcatt acccatgatg cagcgaccta ggcatctaaa atatagcgcg
     1201 accgctggac gctatcgcgc tgatgcaaac agtgatagca aggaacccga atttgctgaa
     1261 gccacggcaa tatatggttt gaataatact tttacgctgt atggcggcct gctcggttct
     1321 gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact gggcgcgttg
     1381 tcgatggata tcaacagagc tgacacccaa ttcgataacc agcactcttt tcatggctat
     1441 caatggcgta cgcagtacat caaagatatc ccggaaacca acaccaatat cgctgttagc
     1501 tactatcgct ataccaacga tggctatttt agttttgatg aagccaatac ccgcaattgg
     1561 gactataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac aatatttgat
     1621 ggggtaagtc tgtatgcctc cggttcgcag caagactatt ggggcaataa cgagaaaaac
     1681 aggaatatct ctgttggggt ttccggcctg caatggggaa ttggttacag cctgaattat
     1741 caatacagcc gctacactga tgaaaataat gaccgtgcac tctctttgaa tctcagtatt
     1801 ccgttagaac gctggttacc gcgtagccgg gtttcctatc agatgaccag ccagaaagat
     1861 cgcccaaccc aacatgaaat gcgtcttgat ggctcactgc tggatgatgg tcgcctgagc
     1921 tatagcctgg aacaaagtct ggatgacgat aacaaccata acagtagcct gaacgccagt
     1981 taccgttcac cttatggaac cttcagtgcc ggatacagtt acggtaatga cagcagccaa
     2041 tacaattacg gcgttaccgg cggcgtggtt atccatcctc atggcgtgac gctctcgcaa
     2101 tatctgggca acgcttttgc gcttatcgat gctaacgggg cttctggcgt gaggatacaa
     2161 aactatccgg ggattgctac tgatcccttt ggctatgcag tgattcctta tctcacgact
     2221 tatcaggaaa accgtctctc ggtagatact acgcagctgc ccgataacgt cgatcttgaa
     2281 caaacaacac agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc
     2341 aatatcggtt atcgcgtact tgttacagtc agcgatcgca acggtaaacc gttgcccttt
     2401 ggcgctcttg ccagcaacga tgatacgggg caacaaagta tcgtcgatga gggcggcata
     2461 ctatatctct ctgggatatc gagtaaatca caaagctgga ctgtacgctg gggaaatcag
     2521 gcagatcaac aatgtcagtt tgcttttaat acaccggatt cagaaccaac aacctctgta
     2581 ttacaaggca cagcgcagtg ccattaagga taaaaaaatg aaaagagcgc ctcttataac
     2641 aggacttttg ttgatatcca catcctgcgc ttatgcctcc tcaggagggt gtggagccga
     2701 cagcactagc ggtgcgacaa attacagcag tgtggttgat gatgttacgg tgaaccagac
     2761 agataacgtg acaggacggg agtttacctc tgcaacgcta agtagcacta actggcaata
     2821 cgcctgttcc tgctctgcgg gtaaggcagt taaacttgtc tatatggtca gccccgtact
     2881 taccaccact ggacatcaga caggat
//
LOCUS       DAJVOY010000282         2892 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVOY010000282 DAJVOY010000000
VERSION     DAJVOY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.285"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1324
                     /locus_tag="OQL90_004050"
     CDS             <1..1324
                     /locus_tag="OQL90_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1370624.1"
                     /translation="GLVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHT
                     MGPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKSDGSLIKEYSVVALQGLTLVL
                     FIFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETF
                     IPHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGS
                     LAMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSI
                     DAPLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDMNNLY
                     KWLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAF
                     ACLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(1325..2233)
                     /locus_tag="OQL90_004051"
     CDS             complement(1325..2233)
                     /locus_tag="OQL90_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1370625.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            2333..>2892
                     /locus_tag="OQL90_004052"
     CDS             2333..>2892
                     /locus_tag="OQL90_004052"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCS1370626.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLS"
BASE COUNT          731 a          617 c          720 g          824 t
ORIGIN      
        1 ggggctggtg gttgtttttt catgggtgtt tatctttgcg ctctatttca caccttatgc
       61 gctaattgtt ggtcagttag gctcgacctt caaagatggg aagggcgggg tcagtacctg
      121 gattaaacac acgatgggac ccggactggc ttatctcgcc gcgtggacct actgggtggt
      181 gcatattccc tatctggcac aaaaacccca ggcaattctg attgcgctcg gttgggcgat
      241 gaaaagcgac ggttcgctaa tcaaagaata ttcagtcgta gcgttacagg ggttaacgct
      301 ggtgctgttt atcttcttta tgtgggttgc ttcacgcggt atgaaatcgc tgaaaatcgt
      361 cggttctgtg gcagggattg cgatgtttgt tatgtcgctc ctgtatgtgg cgatggcggt
      421 aaccgcgcct gcgattactg aagtgcatat tgcgaccaca aacattacct gggaaacgtt
      481 cattcctcat atcgacttta cctacattac cactatttca atgctggttt tcgcggttgg
      541 cggagcagag aagatttctc cttacgttaa tcaaacgcgc aacccaggaa aagaatttcc
      601 aaaagggatg ttatgcctgg cggtgatggt tgcggtttgt gccattctgg gctcactggc
      661 gatggggatg atgtttgact cgcgtaatat cccggatgac ttaatgacta acggtcaata
      721 ttacgccttt cagaagctgg gcgagtatta caacatgggt aatactttaa tggtgattta
      781 cgccattgcg aataccctgg gacaagtagc tgcgttggta ttctcgattg atgccccgct
      841 taaagtgcta ttaggtgatg ctgacagcaa atatattcca gccagtttat gtcgtaccaa
      901 cgcttctggt acgcccgtta atggctattt tctgaccctg gtgcaggtgg caattctgat
      961 tatgctgccg actctcggca tcggtgatat gaacaatctc tataaatggc tgttgaatct
     1021 taactcggta gtgatgccgc tgcgttattt atgggtattt gttgcattta ttgcagtcgt
     1081 tcgcctggcg cagaaatata aaccagaata tgtctttatt cgtaataagc ctctggcaat
     1141 gaccgtcggg atttggtgtt ttgcctttac cgcctttgcc tgtttgacgg ggatcttccc
     1201 gaaaatggaa gccttcactg cagagtggac cttccagttg gcgctgaatg ttgcaacgcc
     1261 gtttgtgctg gtaggattag gactgatttt cccgctgctg gcgcgtaaag cgaatagtaa
     1321 ataattattg tggtcggtga cgctgactct ggctgatctg acccgttttt acataatcga
     1381 gaaatgcgcg caagccgcag gacatatact ggcggtttgg ataatagatc tggaagcctg
     1441 gacgctctgt gctccagtct cccagtacac attctaaacg accagtatca aggtactcct
     1501 tgatctgttc atagagcaga tacccgatac cagcacccat caaaacggcc tccagttcag
     1561 catcaacatc atccagaatg atatttcctg cgacagcaat ttccagctcc tttgcaaatt
     1621 gccagtggaa aggtttgccg ctgggataac gaaaaacaac acattgatga ttaagcagat
     1681 catgaggatg gcggggtttg ccatatcttg caaagtactc tggcgttgct gctacacata
     1741 atttcactgg cgggccgatc gccacgctga tcatatcttt ttcgacgatg ccactaagtc
     1801 gaacgccagc gtcaaagccc tgttggacga tatcgacaag agagtcgtca gttgtgagct
     1861 cgactttaat atcaggatat tcacgtgtga aaccaaccag taaaggcatt aaaaatatac
     1921 gtgcagctac ccgggcgtca ttgattttga gcgttcctgt cggtgtcaga cgaaaatcat
     1981 tcatctcatc taacatgatc tgaatttcgt cgaaagctgg gcgcaatcgt tcatagagat
     2041 tagaacccgc ttccgttaac gatacgcttc gggttgtccg attgaataag cgaattttaa
     2101 gacgttgttc cagtgtttta atactatggc taatggcgga cgaggataag cctaactcat
     2161 cgcccgctgc acgaaagctt tgattacggg ccacggcaaa gaaagtggca aagtcagaca
     2221 tattcatccg cattagtgaa aatccttcat taactcatcc tgaatcatca cgtttacagg
     2281 attatgccgc ctgcgtaaag tgcgctccag aacttaacgt ggaggtaaaa ttatgcagtc
     2341 tgaacgtatt tatttggtat gggcccatcc tcgtcatgat tcattgaccg cacatattgc
     2401 tgatgcgatc catcagcggg caatggagcg gaaaatacag gtgacggaac tcgatttata
     2461 tcggcgtaat ttcaacccag tgatgacgcc ggaagatgaa ccagactgga agaatatgga
     2521 taaacgttat tctccagagg ttcatcagct ttattcagag ctgcttgaac atgacacgtt
     2581 agtggtggtt tttcctctct ggtggtacag cttcccggca atgctaaaag gatatattga
     2641 cagagtatgg aataatgggc tggcttatgg agatgggcac aaattaccat tcaataaagt
     2701 tcgttgggtg gcgctggttg gaggagacaa agaatcattt gtccagatgg gctgggaaaa
     2761 aaatataagc gattatttga aaaatatgtg cagttatctt ggtattgaag atgccgatgt
     2821 cactttcttg tgtaatacag tggtattcga tggggaagaa cttcatgcga gctattatca
     2881 gtcgttatta tc
//
LOCUS       DAJVOY010000283         2863 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVOY010000283 DAJVOY010000000
VERSION     DAJVOY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.286"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..275
                     /locus_tag="OQL90_004053"
     CDS             <1..275
                     /locus_tag="OQL90_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370627.1"
                     /translation="YEGEQLYLNELGYVYGNEEKTNEIVNNNPGYVVEIFEEKMGNEL
                     KIRTRMMEILRDGKINICEYINKEQLEKLNPPEDLRIAIKKLGWNN"
     gene            complement(347..1351)
                     /gene="argF"
                     /locus_tag="OQL90_004054"
     CDS             complement(347..1351)
                     /gene="argF"
                     /locus_tag="OQL90_004054"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1370628.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1513..1929
                     /gene="rraB"
                     /locus_tag="OQL90_004055"
     CDS             1513..1929
                     /gene="rraB"
                     /locus_tag="OQL90_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1370629.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1975..2477)
                     /locus_tag="OQL90_004056"
                     /pseudo
     CDS             complement(1975..2477)
                     /locus_tag="OQL90_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2670..>2863
                     /locus_tag="OQL90_004057"
     CDS             2670..>2863
                     /locus_tag="OQL90_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCS1370630.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQR"
BASE COUNT          791 a          633 c          658 g          781 t
ORIGIN      
        1 agtatgaggg cgaacagctg tatcttaatg agttaggata tgtttatggt aatgaagaaa
       61 aaacaaatga aattgtaaat aacaatccgg gttatgtcgt tgagattttt gaagaaaaga
      121 tgggtaatga attaaaaatt agaacaagaa tgatggaaat cctccgggat gggaaaataa
      181 atatatgtga atatattaat aaagaacagt tagaaaaatt aaatcctccg gaagatttac
      241 gtatagcaat aaaaaaattg gggtggaata attgaactat tactgcataa aataatgtga
      301 tgccgggatg tttttgtatt tcccggcatc tttattgcga tatcaattat ttactgagcg
      361 tcgcgaccat caccgctttg atggtatgca tgcggttttc cgcctgatca aaaacaatgc
      421 tggcggcaga ttcgaagacc tcatcagtca cttccattcc gccatgtagg ccaaactctt
      481 ccgccatttt cttaccaagc gtcgtctggt cgtcatgaaa cgcgggcagg cagtggagga
      541 atttgacctc cgggttaccg gtcaactgca tcatcttgct gttcacctga taatcacgca
      601 gtaatgcaat ccgttctgcc catttctctt ttgcttcccc catcgacacc catacatcgg
      661 tatagataaa gtcagcacct tcaactcctt tggctacatc ttccgtcagc gtaatattcc
      721 cgccattttg ctgtgccaag gcgcggcatt ccgtaaccag cgcagcttcc ggccagcatg
      781 cctgtggcgc gaccagacgc aaatccagac cggtaagcgc cgcagcttcg agcatcgaat
      841 tgcccatgtt gttacgtgcg tcacctgcat agaccagcgt catttcgttg aatgctttac
      901 cgggtaaatg ctcctgcatg gtgaggagat ccgccagcaa ctgcgtgggg tgaaactcat
      961 tggtcaggcc gttccatacc ggcacgccag cgtattccgc cagtgtttcg gcaatctcct
     1021 gaccatagcc gcgatactga ataccgtcat acatgcgacc cagcacgcgg gcagtgtctt
     1081 taatcgactc tttatgacca atctgactgc cgcttgggcc gagataagta acgcgagcac
     1141 cctggtcata tgcggcaact tcgaaagagc atcgggtacg ggtcgagtct ttttcgaaga
     1201 tgagcgcgat gtttttacca gtgagtttgg cttcttcttt gccgcttttc ttatcggctt
     1261 tcagcttcgc ggctaactgc agcaggctgt tgagttcagc tggcgtgaaa tcgagtaatt
     1321 tcaggaaatg cttatgataa aacccggaca tagatccctc ctgtggctaa cgcctcaatg
     1381 aattaaaatt caatttatat ggatgattat tcatttgcaa gtctaaagca taaatctttg
     1441 tcacaaaggt ggaggcaatg tcagtggtat gtgacaataa gagtatcggc aggacattaa
     1501 gaggaatgag ccatggcaaa cccggaacaa ctggaagaac agcgtgaaga aactcgtttg
     1561 attattgaag aattactgga agatggcagc gatccggacg cgctgtacac catcgaacac
     1621 catctttccg cagacgatct ggaaaccctg gaaaaagcag cagttgaagc gtttaaactc
     1681 ggttacgaag tgaccgatcc agaagagctg gaagttgaag atggtgatat cgtgatttgc
     1741 tgcgacatcc tcagcgagtg cgcgttgaat gccgatctga tcgatgccca ggttgaacaa
     1801 ctgatgacat tggcggagaa atttgacgtt gagtacgacg gttggggcac ttattttgaa
     1861 gatcctaatg gcgaagacgg cgacgatgaa gattttgtcg atgaagacga tgacggggtt
     1921 cgccactaat taattgatta aaatgcccga tagcacacac tagcgggcat ttttttagag
     1981 ttcgcgcaac atccgcactt cacaatcaac atggcccgtg cagccaagtg catagtcgat
     2041 atgctcaaag cccaaatgct cataaagcgc aatggcttcc tttaaaaagc ggtcgtttcc
     2101 agatagcagc gtttgaagcc catctctcgt gcctgctcca tcgccattaa ggccagtttt
     2161 ttcgccagcc ctttaccgcg aatagccgga agaaaataca tcttttgcag ttcgcaaatg
     2221 tccgattcac tacctgctaa tggcgcaatc ccaccgccgc cgaccacttc accctcgtac
     2281 tcaaccaccc aatatgcatg acccggctga ctatacactt gatacagttc gtcaagattc
     2341 ggatcggcga cggtgtagcc tttatcagcg gtaagaccgt attcggcgga tacctgacga
     2401 atgacgcggg caatagcagg attatcctgt agcgtcaggc gacgcattac aggtgattgc
     2461 ggcgcaatgt tattcatagt ttaacccaat aaaaataaag taattataca tttgtttaat
     2521 accactctct tcttacgtgc gcaagcatct tataaagcaa taaagttttt gccagcctat
     2581 tactgataag agtattgtaa cgtggcagaa cattttactg tctgtatata taaattatat
     2641 caatggattt tttaattcag ggaattttta tggctcaagt tattaatgaa atggatgttc
     2701 cgtcccattc gtttgttttt catggtacag gtgagagata ttttcttatt tgtgtggtga
     2761 atgtgttgtt aacgattata acgctaggta tctatttacc atgggcatta atgaaatgta
     2821 agcgttatct ctatgctaat atggaagtta acggacaacg att
//
LOCUS       DAJVOY010000284         2844 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVOY010000284 DAJVOY010000000
VERSION     DAJVOY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.287"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..414)
                     /locus_tag="OQL90_004058"
     CDS             complement(<1..414)
                     /locus_tag="OQL90_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1370631.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWALIPPPSYEELIQQAESERQLLLNQANEYMNSKQWPGKAAI
                     GRLKGEELAQYNLWLDYLDALELIDTSSAPDIEWPT"
     gene            complement(416..802)
                     /locus_tag="OQL90_004059"
     CDS             complement(416..802)
                     /locus_tag="OQL90_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370632.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            833..1399
                     /locus_tag="OQL90_004060"
     CDS             833..1399
                     /locus_tag="OQL90_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1370633.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1542..2714
                     /locus_tag="OQL90_004061"
     CDS             1542..2714
                     /locus_tag="OQL90_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCS1370634.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            2724..>2844
                     /locus_tag="OQL90_004062"
     CDS             2724..>2844
                     /locus_tag="OQL90_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCS1370635.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRG"
BASE COUNT          747 a          704 c          730 g          663 t
ORIGIN      
        1 cgtaggccat tcaatatctg gagcactgga ggtatcaatc agttccagtg cgtccagata
       61 atccagccac aaattatatt gcgccagttc ctcacctttc agacgaccaa tagcggcttt
      121 accgggccat tgtttactgt tcatgtattc gttggcctga ttaagcaata attgcctttc
      181 tgattctgcc tgttgaataa gttcttcata tgatggtgga ggtattagtg cccatgtggg
      241 taatccattt tttcctgcaa cacgaatttt gtcatctggg ggcgtattga ttgcaaattc
      301 attataaaca tcatcactga cagccagagc atcatctggc catgaatttg cattaatgta
      361 atcatcctta agcgcaggat tcacaaaaat gtttaaagat ggactataaa acatattaaa
      421 cccctatagc gatataacga cctaatacag catttgcagc agtcgctatg cttgaaaaac
      481 cgcggaactg atttgatgta attgcagaag ccgacaggat tcctgcgcct gaaggtgtat
      541 gccccacatg actaactatc atcccataac atgctgacgg aaaggcaaaa ggaaaatcat
      601 taagatatcc tgcatcttca ccaccagacc caccaaatct cgcctgcccc cactgaataa
      661 tcagtgttct ccgggaacct gaaataatta acggaatcgt tacataccca ttcaggccaa
      721 tgacacccga tgcagtgcca gccagagata attctcccaa accaacgttt aagaaaatgc
      781 agagataatg gctaactggc atcatccccg gtttttattc aggggattga tcatgcttat
      841 tggctatgta cgcgtgtcaa caaatgacca gaacaccgat ttgcaacgta atgcactgaa
      901 ctgcgcggga tgtgagcgga tttttgagga taaaatcagt ggcactaagc ccgacagacc
      961 ggggcttaaa aaactgctca ggacactatc ggcaggagac acgctggttg tctggaagct
     1021 ggacagactg ggacgcagta tgcggcatct tgttacgctg atagaagagt tgcgccagcg
     1081 tggtgtgaat ttccgaagcc tgactgacag tattgatacc agtaccccaa tgggccgttt
     1141 cttttttcac gtcatggggg ctctggctga aatggaacgt gaactgatag ttgaacgtac
     1201 cagagcaggg ctggctgcag ctcgtgccaa aggcagagta ggtggacgcc gtcctaagtt
     1261 gaccaccgaa cagtgggcac agattggacg tttactcgag gccggagaat caagacagcg
     1321 tattgcactg atttttgatg tgggcgtttc taccatttat agaaaatttc cggcgaatga
     1381 gagcaatgaa tcgccctgag tcagtattat tttgattttt cctgtaagcg gacaaatacc
     1441 gtcattttgt gtgaataacg acacaactgc gcttagctgt ttgtcaggca caatcacttc
     1501 aacatagggc gaagcctaat ccaatcagga ggttcgccac tatggctcag gattaccacc
     1561 acggggtgcg cgttgttgaa gtcaacgaag gcacccgatc cattaccacg gtgagcaccg
     1621 ccatcgtggg tatggtctgc acgggcgatg atgccgatgc aaaaatgttt cctcttaata
     1681 aacctgtgct gatcactgat gtgctgaccg ccagcggtaa agcgggtgag tctggcacgc
     1741 tggcccgttc gctggatgcc atcgctgacc aggcaaaacc cgtgaccgtt gttgtgcgtg
     1801 tgccgcaggg tgaaacggaa gaagaaacca caaccaatat cattggcgca gtgaccgccg
     1861 agggtaaaaa aacaggtatg aaagccctgt tatctgccca gtcacagctc ggcgttaaac
     1921 cgcgcattct cggcgtgcca ggtcacgaca cgaaggcggt agctactgag ttgctgagcg
     1981 tggcgcaaag cctgcgtgga tttgcttacc tgtcagcgta tggttgcaag acggtgcagg
     2041 aagcaatcac ttaccgtgaa aacttcagtc agcgtgaagg aatgctgatc tggcccgact
     2101 ttactggctg ggacacggtg ctgaatgccg aagcaacggc ttatgccacc gcccgtgcgc
     2161 tcggtctgcg cgctaaaatt gatgagcaga ccggatggca caaaagcctg tccaacgtgg
     2221 gcgtgaacgg tgtcaccgga atttctgctg atgtgttctg ggatctgcag gacccggcaa
     2281 ccgatgcagg tctgcttaac cagaacgata tcaccacgct tatccgcaaa gacggtttcc
     2341 gcttctgggg ttcccgctgt ctgagcgatg atccgctctt tgccttcgaa aactacaccc
     2401 gcacggcgca ggtgctgatg gacacgatgg cagaagcgca catgtgggcg gtggacaaac
     2461 cgctgaaccc gtcgctggcc cgcgacatta tcgaaggtat ccgcgccaaa atgcgtagcc
     2521 tggtcagtca gggctatctc attggtggtg attgctggct ggacgagtcg gtgaacgata
     2581 aagacacgct gaaagccggg aaactcacca tcgattacga ctacacgcca gtgccgccac
     2641 ttgaaaacct gatgctgcgt cagcgcatca ccgatcagta cctggtgaat ttctccagcc
     2701 aggtcagcgc gtaaggggac aacatggctt taccacgcaa attaaaacac ctgaacctgt
     2761 ttaacgacgg gaacaactgg caggggatcg ttgagtcgct gacgctgccg aaattcaccc
     2821 gcaaatatga gaagtatcgc ggcg
//
LOCUS       DAJVOY010000285         2832 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVOY010000285 DAJVOY010000000
VERSION     DAJVOY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.288"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..136
                     /locus_tag="OQL90_004063"
                     /pseudo
     CDS             1..136
                     /locus_tag="OQL90_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            386..1252
                     /gene="ospB"
                     /locus_tag="OQL90_004064"
     CDS             386..1252
                     /gene="ospB"
                     /locus_tag="OQL90_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCS1370636.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1582..2322
                     /gene="phoN2"
                     /locus_tag="OQL90_004065"
     CDS             1582..2322
                     /gene="phoN2"
                     /locus_tag="OQL90_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCS1370637.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(2675..2832)
                     /locus_tag="OQL90_004066"
                     /pseudo
     CDS             complement(2675..2832)
                     /locus_tag="OQL90_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT          928 a          515 c          526 g          863 t
ORIGIN      
        1 agcggtaaga gaagttcatc atacttggcg cagttacgcg atgtttctgc tagaggtgat
       61 gccgtaaaga acggcagaac attaccggaa caagatagcg gtctacctgc actggtatca
      121 gacccggggc ttccccggat gatctccacg gtactttagg tactgtgtct atattatgta
      181 ggtaggatca attaactttg tagttgaaat tctttcattg aaataatgaa taaaaaagac
      241 aaaccgcatt aaacaatgcc agaatataat tgcatttttg ttccgttttt ttaaatcata
      301 gtatacaaag aagcttgcat actatatgaa aatataacac cctttactat gatgtttata
      361 aaacaatata tggagtcatg taggtatgaa tttagatggt gttagaccat actgtagaac
      421 agtcaataaa aagaatgaaa gcatatcaga tattgcattt gcacatataa taaaaagggt
      481 aaaaaattca tcatgtactc acccaaaagc agcattggtt tttttaggag agaaaggttt
      541 ttgtgatagc aatgatgttc tatctattat gggacaacaa ataccaagag tatttaagaa
      601 caagatgtta tatgattatg tttttaaaaa tgaaaaaagt aaaaatgatt ttctaaaaat
      661 ggctgaatca tggctaccac agagtgaacc aatagtaata aataatgatg atgacgcatt
      721 gaatgctgct gcttattttt ctgtaaaaaa agcgaaaata aaaacagtaa acgatactga
      781 ttttaaagag tataataagg tttatattct tgggcacggt agtcctggtt ctcatcaatt
      841 aggccttggt tcggaactta ttgatgtaca aacaatcatt tcaagaatga aagactgtgg
      901 tattctaaat gtgaaagata tccgttttac ttcatgcggc tccgctgata aagtggctcc
      961 taaaaatttt aacaatgccc ctgctgaaag tctttcttgt atccttaact ctctgccttt
     1021 ttttaaggaa aaagaatctt tgctagagca gataaaaaaa caccttgaaa acgatgagtc
     1081 attgagtgat ggtctaaaaa tatccggcta tcatggatat ggagttcact atggtcaaga
     1141 gctttttccc tactcacatt atcgttcaac ttcaattcct gctgatccgg agcatacagt
     1201 aaaaagaagc tctcagaaaa agacttttat tattaataaa gaactggatt agtataaaat
     1261 ttttaaccta tagttaattg tttgcattta agatgaagtt atttttttct ttagtagaaa
     1321 agtgcttccc tatgagggaa aatcacaaat catcataata cagagacaaa acgatacgaa
     1381 caaatagata aaaaacatca ttcttttact acattatttt tcgtgaatat gaaaaataat
     1441 gttattactt taatataaga ctattttttg tttttccatc actctgttca aatttttccg
     1501 catgacttgt gttttttgta acacagctcg ttttttacag ctgaccaaaa tcatcaatta
     1561 attatgctaa ggaaataaat tatgaaaacc aaaaactttc ttcttttttg tattgctaca
     1621 aatatgattt ttatcccctc agcaaatgct ctgaaggcag aaggttttct cactcaacaa
     1681 acttcaccag acagtttgtc aatacttccg ccgcctccgg cagaggattc agtagtattt
     1741 caggctgaca aagctcatta tgaattcggc cgctcgctcc gggatgctaa tcgtgtacgt
     1801 ctcgctagcg aagatgcata ctacgagaat tttggtcttg cattttcaga tgcttatggc
     1861 atggatattt caagggaaaa taccccaatc ttatatcagt tgttaacaca agtactacag
     1921 gatagccatg attacgccgt gcgtaacgcc aaagaatatt ataaaagagt tcgtccattc
     1981 gttatttata aagacgcaac ctgtacacct gataaagatg agaacatggc tatcactggc
     2041 tcttatccct ctggtcatgc atcctttggt tgggcagtag cactgatact tgcggagatt
     2101 aatcctcaac gtaaagcgga aatacttcga cgtggatatg agtttggaga aagtcgggtc
     2161 atctgcggtg cgcattggca aagcgatgta gaggctgggc gtttaatggg agcatcggtt
     2221 gttgcagtac ttcataatac acctgaattt accaaaagcc ttagcgaagc caaaaaagag
     2281 tttgaagaat taaatactcc taccaatgaa ctgaccccat aaagctggac agcctgtatc
     2341 aggctatgga gggcccatag acaaatctac cctatatgag cataggagga gtctatgggc
     2401 acaccacgtt ttacccctga atttaaggga ttactggaaa ggctgggaca tatccctccg
     2461 gcagaagcag aaaaagctta ttatgctgcc atcggaaacg atgatctggc aacctgagtt
     2521 cacagataaa acattctcta ggaaactcgg ggcggttccg ttcaccacat gcaatgtggt
     2581 gttgcagggg aacggtctgc accatcccct atgtcgatca atataacaga aatgacaact
     2641 tcagattcag ggcacaacct aaatatattt taggtcacct ctcaaatcgt ttgcctgata
     2701 ccgctccatt ttttaataaa aaaatcaatc atttttgaat ctttgctttt cattgcattg
     2761 tcaagcatag tgtccccact atttgctttt tgaaattttt tgtttacatc aaccaatcca
     2821 ttattgataa aa
//
LOCUS       DAJVOY010000286         2792 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVOY010000286 DAJVOY010000000
VERSION     DAJVOY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.289"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..534)
                     /locus_tag="OQL90_004067"
     CDS             complement(<1..534)
                     /locus_tag="OQL90_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1370638.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPI"
     gene            complement(535..2034)
                     /locus_tag="OQL90_004068"
     CDS             complement(535..2034)
                     /locus_tag="OQL90_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1370639.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(2095..>2792)
                     /locus_tag="OQL90_004069"
     CDS             complement(2095..>2792)
                     /locus_tag="OQL90_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1370640.1"
                     /translation="QDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIKADKAGIP
                     VITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQSVVARVD
                     GFKKGLSDTPDIKIVAEQPSITRAEALTTAQNIMQANANLNGLFGFGDDAALAAVIAA
                     KSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNGKPVE
                     KMIPVQPGVYTGK"
BASE COUNT          774 a          692 c          548 g          778 t
ORIGIN      
        1 aataggtata tagagaaatt ctccattccc cagtgccaga aaggcatcat ctttaatcat
       61 gatgggcata ccatcggtca ctatataagc gataccacgg aagatcccca tagtagcaac
      121 ggtgacaata aacgacggga tccccgctat agccgtcaag ccaccattta tcatccccag
      181 agccagtgct gcaataatgg ataatgggac caataaaata aatggaattc cccatgaagc
      241 cccaagcgca gcaaatgtgc caatcaatgc aatggttgaa ccgaccgata aatcaatatc
      301 gccaagcaaa agtacataag tcattccata ggcggtaatt gcaattatcg aaacctgaag
      361 aataatatta gtaatgttgt tggcggtaaa aaaactgtca ttaataaaac tgaacagaat
      421 gattaacacc accattccac tgaggatacc gccatattgc ctgaagactt ttttaaaatt
      481 atttgcaatg ggaatttccg aaggtttcgg actggcaaga gttttctctg tcatttaacg
      541 tactcctgtt gcagcggtca ttacctgtgt ctgcgttaaa ttttcacatg aataaatatc
      601 ggcaatcgtt ttattacgca ttaccaccag acgatcgcta agtgccatga cttctggcag
      661 ttcagaggac accagaataa ttgctgtccc tgccgcagcc agttgacgta tcacgttata
      721 gatttcaaat tttgcgccga catccactcc gcgcgttggc tcatcgagca ataaaatacg
      781 cggagttttc tccagccact tcgataacac gattttctgt tgattaccgc cgctcagcgc
      841 tcccactgcc tgatgtacag aactcacttt agtgttcatt ctggcaattg attgcagcgt
      901 cttcgcctct tcttgtcggt gattgatgag tcccatccgc gaagcgttat attgaaacgc
      961 cacatggtgc gtgttcatat tgacgtcgtg catcagcact aacccttctt ccttgcggtt
     1021 ttcggtcaca aaagcaatgc catgatcaat agcctgttta ggattagtga tcctgacttc
     1081 cttaccatca atcaacacag tcccctgata cgaaacgagg ccaaagagcg ccttcatcac
     1141 atcggaacgc ccggccccca ccaggccaaa aaatccgagg acctcccccg gacgcacagc
     1201 gaaactgatc tctttaaaaa gcgaaggatg ggaaagattt tttacttcca gttttgcagg
     1261 aacattttca tccggtattt ccccaagacg tgctggccaa atatttttca tttcccgacc
     1321 aaccatttgg gcaatcaaat gctggatatc actgtttttc ttttcatgcg tggcgatata
     1381 ctcaccgtcg cggatcaccg aaatatcatc tgaaatccgc ataatttcat ccatgcgatg
     1441 agagatataa ataaccgcac agcctcgctg ctttaatttt tcaataatgt taaacagaat
     1501 ctccgcttca ttatcactta gcgaagatgt cggttcatcc ataatcacga catccgctgc
     1561 atagcttaag gctttagcaa tttctaccag ttgcctatgt gccatcgaca tattgccgac
     1621 catttctagt gggtctagtg gtatgccccg ttccgcgagg agattctggc aatctgcgag
     1681 cattttttta tcgttaataa tgccaaaacg tcgcggttca ttattggcat aaatattttc
     1741 cgccacggtc atattattac tcaggctcaa ttcctgaaaa ataatcgcaa ttcctaactg
     1801 gcgtgaatgc tgcggattat taatggtgat agtttcacca cgtagtgtta tcgttccctg
     1861 ggttggcgga taaacaccgg ccagtatttt cattaatgtc gatttaccgg caccattctc
     1921 gcctaataaa gtatgcaccc gccctttacg taaagagagg gtaatatctt ttaatgcttg
     1981 aacatgacca aatgtcttcg caatatgcga aacatcaaga ataacatccg tcataatgac
     2041 ctcggtgtca caacaaaacg ccaataacgt tttgttgtga ggtgaagatg tttactattt
     2101 acctgtataa acgccgggtt gtaccgggat cattttttct accggtttac cattgaggtg
     2161 atcaacagcg gcatcaatgg ctttagcacc catttgatcg ggatattgcc gaatcacggc
     2221 cgcaaacgtt ttttcactgt caacggcatc acgcgcttct ttcatgccat caaaaccaat
     2281 gattttgatg ttgtcgttat gcgcagattt tgctgcgata accgctgcca gcgccgcatc
     2341 atcaccaaag ccaaacagac cattcaggtt ggcatttgcc tgcataatgt tttgcgcagt
     2401 cgttaacgct tcagcccgcg taatactagg ctgttcagca acaattttaa tatccggtgt
     2461 atcgcttaac cctttcttaa aaccatcaac gcgcgcaacc accgattgta gtgccggata
     2521 agaaataatg gctacagtac ctttaccatt caaaagtttg cccattaact tgcctgcttc
     2581 aacgcctccc gcatagttat cggtcgcaac atgcgatacg accggaacgc cttccgcagc
     2641 gacgtcgacg gtgataacgg gaataccggc cttatcggct ttaattatcg ccgcctggac
     2701 gcctttcgaa tcgaccggag agataataat ggcatccact tttttagtaa taaaatcctc
     2761 aacatcagaa agttgtttgt ttaaatcctg at
//
LOCUS       DAJVOY010000287         2720 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVOY010000287 DAJVOY010000000
VERSION     DAJVOY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.290"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..2634)
                     /locus_tag="OQL90_004070"
                     /pseudo
     CDS             complement(1..2634)
                     /locus_tag="OQL90_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          629 a          682 c          662 g          747 t
ORIGIN      
        1 ctgttgcctg cggtgatgtc ataccccagt atcatgctgg cacgccccag cagcgattcg
       61 tagtgattca gatgtatatt gaggccatta ctcgccttca tagccatctc attctggtgg
      121 ctgtatgtaa gctgggtttg cggctctata tagaacccat aaccagtagg ggacaggttg
      181 aacctctgcc cggcttctag ggagatgctc attccattcg cagtgccgtt ggcgttaacg
      241 ccgttgttat gactgtccag tacgtggaaa ctatttttct ggcgcgatgc ttttataacg
      301 agatcgctgt aaaaaccgtt ttgtgccatg taactggcgt acattcccat gtagtctgaa
      361 cgtgcggtac cgtcgcctcc gctatagtcc ggcgatgcat gtgttgagcc aatatacaga
      421 ccgacataca acggcatttc atcagagaga cgtttatccc caccaaactg gataccgctg
      481 taacccatgt caaagccgct cagtttgcca ctggcaaagg agtccaggct tcccccataa
      541 ctgcgcaacc agatattacc gtctttactc tgatttcgca ggtcacccat ccgttgcatc
      601 agcgtacggt tttcaacata gttcaataag taaccgacat tgagataatt gccgccagca
      661 tctgcggtcg tggtgggttt aggcgtggga gcgggttcag gcgtaggatc ggggttgact
      721 atgggaggct gagcgggagc tggtgtgggt tcaggattag gagtcggttc cggaactgtc
      781 ccggaagcgt aaagctccca gttagtgcca tttttacgca catcgtacag atatcccccc
      841 caactcaacc tgagaagacg cgctgaacga ggccgcgccg tcagtggttt ttaccactgt
      901 cagaacttca tttcccgttg tggcctcgct gccctggttg cggatagcca atacgtgatt
      961 accagcacta ctcccgctga tattcaataa atccccttta ttattaacgc cattaccctc
     1021 gccaacaaca tcagcacgca taataaaggt actgttaccg ctcaggttct ctacgtttaa
     1081 tgtggcaaat gtgccggcag ttgacccgtg gctggcaaaa tcgacagttg aatggctcag
     1141 cgccagcgtg tcgagattag aattacttgt tacgttccag acgctgttat tcattgcaac
     1201 gtccagtttt ccgccattga cattatcgct gagggaggaa cctgtccaaa ccgacccagg
     1261 gtgcatatcc agattgatta gcccaccttt ggaaaaaatg ctaccgttga taagcatacg
     1321 acccgaggcg ttgatgcggc tggcggcata accatcgtca tgttgcgttg caatggccat
     1381 cttgtcgggt gtactcatat caatgaccag atcgctcgtg aggtcgatct ggctgttggt
     1441 catggcataa attcccctgg ctcctgccgc gccggtgata gccaaactgt ctccggttat
     1501 tctaccgccg ctgagcgccc acaaacccag cgccagactg ccattacgat caactgtaat
     1561 atcggtattt tgcatgttga caacagccgt tgccgtctgg gccgaggcac cataagaacc
     1621 gccggaaaag atgctgtttc gttgcgctgc cgtgccagaa aaattgattg tcgcgtctga
     1681 accactggtg acgaggccac cgccctgcgc ggaagaaata tggctatctg caccgatagt
     1741 ggttgtacca ccacgaactt cgacgccatt ggccgcggct ccagttacat caacagtgag
     1801 tgcattcgcg ctaacctggc caaagctcca gaggccgtgt gcattatcgc cattggtttt
     1861 aatggaactg tttgttccga gatcgacaac agagtttttc tgtacgttta tccccatggc
     1921 gctgtagccc tgaacatcga ttgtcaggtc tgtagccgta aaacgcgcag caccattggc
     1981 gttattgccg ttgagaccac cgatataaac acctgtactt ccatcggtcg tgattttact
     2041 tccgcttcca agatcgacac tggtgccata gtcattgatg gttaggccta taccgttcga
     2101 gttttcaatg gtgaattgag tggctgttag tgttgagcta tgcccaataa tgatgccgtc
     2161 atcgttactc ttaatggtgc tgcctgtgcc taagtcagca tgggtatagt caccaattaa
     2221 gttaatgccg atggcagagg tttgaccaac aacatctact gtgagtcggt tagctgtcag
     2281 ggagctgttt ttgccctgaa taatgattcc ttttgcatat ccagaggcgt cgttgacatt
     2341 gacggtgaca tcattaccca ggttgatatt gccaggcgtg tttcctgctg gggtcattac
     2401 accatagagg tttcctgatg gatcggcggt atctcctgta attctgtcac catcattaat
     2461 agtaatattg gtgccatatt gacttcctgt atcagcagct atcagcaggg gggaaaagat
     2521 aattgcattc aaaccaatag cgccaaattt tatcagttga attgagggcg aacggatttt
     2581 taattcagcg agacgatctg ctttggtatt cccattgtgt tgtttgatgc ccataccact
     2641 cctatatagt acccagacta tgggtctatg gattttgtca ttttgtttaa atcaatgaat
     2701 aatatcctct tatcatcatg
//
LOCUS       DAJVOY010000288         2706 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVOY010000288 DAJVOY010000000
VERSION     DAJVOY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.291"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..187)
                     /locus_tag="OQL90_004071"
     CDS             complement(<1..187)
                     /locus_tag="OQL90_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1370641.1"
                     /translation="MGNYIRPLSDAVFTIASDDQWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFS"
     gene            complement(433..885)
                     /locus_tag="OQL90_004072"
     CDS             complement(433..885)
                     /locus_tag="OQL90_004072"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1370642.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(882..1934)
                     /gene="dinB"
                     /locus_tag="OQL90_004073"
     CDS             complement(882..1934)
                     /gene="dinB"
                     /locus_tag="OQL90_004073"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS1370643.1"
                     /translation="MKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTAN
                     YPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLD
                     EAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQ
                     FVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFG
                     RILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKV
                     KPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGLH
                     VTLLDPQMERQLVLGL"
     gene            complement(1993..>2706)
                     /locus_tag="OQL90_004074"
     CDS             complement(1993..>2706)
                     /locus_tag="OQL90_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370644.1"
                     /translation="RDNVDAFLTLSATENMEFDSPMQVTTQNMQSGQTVTDNVQRAPR
                     TITISGVVVVGESGSILISRDTKLVENFIDTLENWRDQKQIISVICKDGIKIDDSIIT
                     SFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATGKTATTNDGGATSKKNTGNT
                     TTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASANEQASKVL
                     KSGNALRKYSVNPNKKGTY"
BASE COUNT          701 a          657 c          616 g          732 t
ORIGIN      
        1 cagagaagaa cgctgcgcca atcggatagc cgcgtccggg atgtaaatct ggcatcccca
       61 ctacgcgctg catgttgggt aaatttgccg tggtgtgtaa ttgttggatc gctaaactct
      121 cgatccactg gtcatcagat gcgatggtaa ataccgcatc ggataaggga cgaatataat
      181 tgcccatata ccattccgta aatagactcg tcatacttca agttgcatgt gttgcgactt
      241 gaattattga gagtaacaat taaaaaatca ggaaatggca gacgaatcca gggcaagaaa
      301 aaaataatta atcctggaaa ggtctgcaaa gagtcattga taaaggattt gtcatgattt
      361 acctccttgc agtttgtgga ataagaaaac gcgcacatac taggcttgcg ttattttcat
      421 tgcaagctgg atttaatgtt gctgtttata acgcatataa aaattaataa accactctcc
      481 ccgacattca acgcgttgct gcttaactgt ctgaaaacca taacgttcaa aaaagggttt
      541 tgcggttatg cttgcgtcca ccgtaagttc ggattcagac ttaatcaaag gttttaacaa
      601 agcgctggca accccacggc gggtgtattc agggtcaaca aataacatat cgatatagtg
      661 ttcaacgcag gtaataaagc cgaccagttt tgcattaatg actgcaaccc gcacttgtga
      721 tttcgcgagt ttctccttcc agcgagattc gtcaatctgc gcccaggcgg caatttgttg
      781 tggtgaataa tgctgactgg cagtcatcgt aaccgctcta atgaaaatag cgcatagttg
      841 ctgaaaatcg ccaggctgat agtctcttat ttgtatgtta ttcataatcc cagcacgagt
      901 tgtctttcca tttgcgggtc aagcaacgtc acatgcagcc ccaccagtcg cacaccgcgc
      961 ccgccgcggc gttcatccca ggttttacgc gcggtggcga ttaagtcagc tttattcagc
     1021 cgcggccaga cgtgctcctg agtggtttgc tgaaaatcat caaactttaa tttcaccccc
     1081 tggcgagcaa tcagtaaatc aggtttcacc tttgccagac ggcgttcaag ttccggatac
     1141 agccgctcga taatcgcttc acattcagac cagtggtgga tatcttccgc catcgtgcgt
     1201 tccacgccga cggattttcg caaccgttcg ctgttaacgt cgcgctcgtc aatcccctga
     1261 ctacgctccc acaaaatgcg gccaaatttg ccaaagcgtt taagcagcat caccagatca
     1321 cacttttgta catcaccgca ggttcgtagc cccatcgctt ccagttttgc cgccgagact
     1381 ttgccgacgc cggggatttt tgccagtggt aaggtttgta aaaatgccgg aacttctgcc
     1441 ggcgtaatca caaactggcc gttgggttta ttcatgtcgg aggcgatttt ggcgagaaac
     1501 tttacgggtg ccacgcccgc agacgccgtc agttgcagct cgttgaaaat cgtctggcgg
     1561 atttcctggg cgatgagggt cgcagaaccg tggcaatgga cgctatcggt gacgtcgaga
     1621 taagcctcat ccagtgacaa cggttcaata cgcgaggtgt agcgcgagaa gatttcgcgg
     1681 atatgatttg aggcttcttt gtaggcgtca aagcgccccg gaagcaaggt gagatgcggg
     1741 cataatttga gcgccatccc tgtcggcata gcgctacgta cgccaaattt acgcgcggga
     1801 taattggcgg tactgatcac cccccgacgt tcgcggctgc cgccaatagc aatagggata
     1861 tcgcgcaggg cgggattgtc gcgcatctcc accgccgcga aaaagcagtc catatccaca
     1921 tgaataatct tcactttctt atacaccctg tatatttata catgcaaaga gattatacac
     1981 acaaaagcaa aatcaataag tgcccttctt attcggattc acagagtact tccgtaaagc
     2041 attacccgac ttaagcacct ttgaagcttg ctcattagct gaggctgtga aggtgctctc
     2101 accattttta acgctaacgc tcttagagca cgttacacgg gcttttagcg cttcatctga
     2161 taactcactt gccgaatagc taaaaagctc tttacaggtc agcatagggc ttccaaggct
     2221 cgttgtagtg ttgccagtat tctttttact ggtagcacca ccatcattcg tggtagcagt
     2281 cttgccagtt gcagccgaaa tatcagtctg acccacaata gctttgaagt taatttcctg
     2341 aaaagttagc tggattctta gaccatttga aataccaaca tctttagagg ctttaaagct
     2401 cgtgataatg gaatcatcaa ttttaattcc gtctttacag atgaccgaaa taatctgctt
     2461 ctggtcacgc cagttttcca gagtgtcgat gaagttctcc accagcttag tatctcgtga
     2521 gataaggata ctaccggatt caccaactac aacgacacca ctaatagtga ttgttctggg
     2581 tgctctttgc acattatctg tgacggtttg ccctgattgc atgttctgtg tagtcacctg
     2641 catagggctg tcaaattcca tgttttcagt tgctgataag gttaagaagg catctacatt
     2701 atctct
//
LOCUS       DAJVOY010000289         2679 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVOY010000289 DAJVOY010000000
VERSION     DAJVOY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.292"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            231..770
                     /locus_tag="OQL90_004075"
     CDS             231..770
                     /locus_tag="OQL90_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1370645.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(996..1319)
                     /locus_tag="OQL90_004076"
     CDS             complement(996..1319)
                     /locus_tag="OQL90_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCS1370646.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1603..1797
                     /locus_tag="OQL90_004077"
     CDS             1603..1797
                     /locus_tag="OQL90_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1370647.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          867 a          657 c          568 g          587 t
ORIGIN      
        1 aaaaaaaaac aataaaaaac accgcaaaaa tggattgtta acaaatgaaa atactcaatt
       61 cgaatctcat aacaaacata tcatcaacaa taagaaatca ccttctaaca tcatccaaaa
      121 atataaaaac acacccatca ccaaacatgg acgtaacaag tcatcgtcgt ttattccaga
      181 aagacggaat aggattgagc caaacaggaa aagcaacaca acttgtcatt atgtcgcatg
      241 gtggatggaa agagatctcc catccacaaa cactattcac gcgtcaaaaa ggcgatggat
      301 ggactgtagt ccccaaaaac ctaagaattg atttttatac aaaagacaac gacttcacaa
      361 agggactaag cgttctttca gaggtaaaca aacgccatac tgaagctcaa aaagggttaa
      421 caccatcact aaacataagc aaagatgacc taaaagttct agcgaacgcg cgcaatatac
      481 ctcaatcaaa attagaagaa gaaatgatgt cacaagccat atatagaaaa gaatacgcaa
      541 caagtaatga gaagataaaa aactatgctc tatattacca tgaacaagcc caaaatatta
      601 tacaaagaca tcaagatggc ttaggaaaca aaaatattga tatagcattt ataactgata
      661 aaaatcataa gaagcacctt tctgacatat ttaaagtaat aaatgaatca ggagtaaaat
      721 atgatgtaat tcactttggc gcttgccgag taaatagaga ggagaaataa tcatcattta
      781 ccataatccc ttaattgtac gcaccactga aacgcgctga acgcgattct gacgcagaca
      841 ccacaaaaat agatgtttta ccccggaaaa acaggaagga tgcgtcagga tcgttttcag
      901 ctcgctgcat agctatgcat gaaagcgaat ggagatcact ttgggagctt accgtgttca
      961 taccgtctgt tttcaacagt tttctccccg ggaagctaat ctgccataag cctggataac
     1021 agggcacggt gatactccgt aatagcgatc agcacctccg catactccct gtccccgata
     1081 cgcttgccgt cgataaagag ttttttctgt aacgccccca gtctcataac ttctttcagt
     1141 acccgatcat cagtcagtga gttaacgttc ttaccgagag ctgccgcccg taaaaaaccg
     1201 gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc ttctgccgtc
     1261 agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg ttttgtcata
     1321 aaacatggct ccgtatctga catgggtgtc ggggcaaagc cctgaccagg gcaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt gcacatcctg tcctgtcagc aagctcgaat
     1441 taccgacaaa ggcacggcag ttttttggca gttacagtgt tgtctgacag aaatgcccgt
     1501 caaaaagcca ggcggcagaa tagcggcagc aaaacagtcg aaaagtggta cgcgatataa
     1561 tcacataacg cagatgacag acagaacgga gaaaacgaaa agatgaacaa gcagcagcaa
     1621 gccgtactca acatggcggg attcataaaa agccagagcc tgacgctgct cgaaaaactg
     1681 gacgcactcg atgctgacga gcaggccacc atgtgtgaga aactacacga actcgcagaa
     1741 gaactccaga acagcataca gacacgcttt gaagtagaaa atagcacaga gagataacgc
     1801 tcaggacagg atgtcgaacg gcacaacaga gcgtaaggag aaaatgtcac ataaggcgct
     1861 cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggtat
     1921 cagctcactc aaaggcggta atgcggttat ccacagaatc aggggataac gccggaaaga
     1981 acatgtgagc aaaaagccga aggcagggac aaaggccgcg caggtggcgt ttttccatag
     2041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2221 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2341 tgagtccaac ccggtaagac acgaattatc gccactggca gcagccactg ataaccgaat
     2401 tagaggagta agacttgaag tcatgcgcct gttaaggcta aactgaaaga acaagttttg
     2461 gtgactgcgc tcctccaagc cagttacctc ggctcaaaga gttggtatct ctgagaacct
     2521 tcgaaaaacc gccttgcagg gcggtttttt cgtttacagg cagtagatta cgcgcagaaa
     2581 aaaaggatct caagaagaac ctttaatttt ttttcacact tggtatccta ctttaaccca
     2641 taaaataaat acttacaccg acacgccctt aaacaaaag
//
LOCUS       DAJVOY010000290         2640 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVOY010000290 DAJVOY010000000
VERSION     DAJVOY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.293"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            232..543
                     /locus_tag="OQL90_004078"
     CDS             232..543
                     /locus_tag="OQL90_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1370648.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            543..830
                     /locus_tag="OQL90_004079"
     CDS             543..830
                     /locus_tag="OQL90_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1370649.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1367..2554)
                     /locus_tag="OQL90_004080"
     CDS             complement(1367..2554)
                     /locus_tag="OQL90_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1370650.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
BASE COUNT          676 a          708 c          626 g          630 t
ORIGIN      
        1 aaggtgaaat ctgtcaatca gtaccggctt acgccgggct tcggcggttt tactcctgtg
       61 ccatatgcaa caacggagtg ccgccttcca tgccgctaac gcggcatcaa aaaatccctc
      121 ttctccagtt gcccccctcc gaactcagag tgtattttca tttattgatg aaatttactg
      181 tttgcgcaat tttcctgttt tagtgtattt tgtcattctg gaggctataa tatgtttaat
      241 gtgattactc acccggccgc actggatgaa cttctagaat taccagacaa tctgcgtggt
      301 cgtatgacca gactgattga acggctggaa caggaaggta acaaaatgaa aatgcctcac
      361 agccgtgtaa ttggcggagg gctttttgaa ctgagagtcg gggataaaaa tatcgcaagg
      421 acactgtacg cttatgcaac gggtaacgaa atctatctgc tgcatgcatt tgtgaaaaaa
      481 acgcaaaaga caccaacgaa agctattgaa attgccagga tgcgtctgaa ggagatgaac
      541 tgatgaaagt aaaaggtgtt ccattcagcc aggtaaagca aaaactactc agtactccag
      601 aagctgtcaa agggtatgag gatgctgaca aagaactgga gatggtggaa atgttatacg
      661 aaatgagaga aaaagcggga ttaacaaagt cagcactggc cgaacgaatg gggttacgcc
      721 cgtcagccat cagtcgcctg gaaagtaacc ctttaggggc cagcatgaaa actttgacaa
      781 aatatgctca tgcctgtggc gcaaccatcg atattcacgc tgtctattga ggggtccgct
      841 tggaattcgg aaaataaacc acgttaagac tattttttag ataaatgact ttcaggaatc
      901 gccaagatgg cgggataaca gcggttttgg tgtcctaaat cgtacaattc tgtacatcca
      961 cgcaagggaa tgtacggaag taagccagac ggttatcgga cggtcagaac atttcgtaca
     1021 gcctttacgt tcagtgagtt agctggcctg gacgtaacca ctcgttagct caattctctt
     1081 agcgtgggaa aatttccgaa ttccaagcgg acccctttat ccttgccccg ctgaatgagg
     1141 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc atcgatagga attaaaaccc
     1201 caaaaagatt aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctcatatg
     1261 aacggaaccg acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt
     1321 ttgctccgtt tccctcaaaa tggacgctac ttaccctctg cggctctcag ccgcaccacc
     1381 gcatccgggc cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca
     1441 gccccgtaaa acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta
     1501 acctctgata catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta
     1561 tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg actcagaaaa ccgtagtacc
     1621 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccatg aactcatctc
     1681 cactcatcaa caggtattct tcccgttttg ttcggtgact gttgtaacgc agtccgattt
     1741 catcctgacc ggcataatgc tccagacgac tcaccggcac cggtggcttt ttcaggtaag
     1801 agccaaagta caccgccaca tgggtggcat tatccatcac ccgggatacg ttgacattcc
     1861 agccacggcg gtaatgcgtg tccaggaagc gattccattc ccgtttactg cttccttctg
     1921 ctgccagcgc atccggcatc accaggtcag ggtatttccg tgacagtaac cgtgttatcc
     1981 ggtagcgcca catgctcatc accttacggg cgtaaaaatg aagatttttc caggtgtggc
     2041 ccgacgtcac accaccggca gttgtcgata aatggatatg cggatgccac tgctggtcac
     2101 gcccccatgt gtggatcacc gtgaatatcc tcggctccac atctgcctga tggcagattt
     2161 ccagtatcac atccgctgca atgcggctca tctctgccag caaccaccgg ttgtggaaca
     2221 ccagggacca gtactggcag ggtaacgtga acacaatatg ctgccatggg cagtcgggga
     2281 ccaggctcag cagatactgt atccactgtg cgccagcctt caccccgcag tgcgggcagg
     2341 agcggctttt acaccggaag cagacctttt ttgtatggca acagtccggt gatgaacagc
     2401 accactgtgt ataccccatc agtgtggtcc cgcacgccat gattttggtc accgactcaa
     2461 tcaccaccgg acgtactgcc ccttccggct gcttctccag ccagttaagc cagcggtttc
     2521 cctgctgaaa gatatcggca aaacggggaa gcatcagaag ggcggggcga ctccgtccgg
     2581 ccagtgaacc gtgccacact ccgggcagta cataccgccg gcgctgatac cggaaagaat
//
LOCUS       DAJVOY010000291         2539 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVOY010000291 DAJVOY010000000
VERSION     DAJVOY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.294"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(38..2416)
                     /gene="ppsA"
                     /locus_tag="OQL90_004081"
     CDS             complement(38..2416)
                     /gene="ppsA"
                     /locus_tag="OQL90_004081"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1370651.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNGRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          586 a          729 c          636 g          588 t
ORIGIN      
        1 cataaacccg gcgacaaaac gccgccgggt atttatttta tttcttcagt tcagccaggc
       61 ttaaccaggt ttgcaccacg gtgtccggat tcagagacag gctatcgatc ccctcttcca
      121 tcaaccatgc ggcaaaatct tcgtggtcgg acggaccctg accgcaaatc ccgacatatt
      181 tgccctgttt cttcgcggca cggatagcca tcgacagcaa tgctttcacc gcatcgttgc
      241 gctcatcgaa cagttcagac accacgccgg agtcacgatc cagaccgagc gccaactgcg
      301 tcatgtcgtt tgagccaatt gagaagccgt cgaaatattc gaggaactgc tcggccagca
      361 aggcgttgga cgggatttca cacatcatga tgattttcag cccgttctca ccacgcttca
      421 gcccctgacg cgccagttct tcaaccaccg ctttcgcctg gtctacggta cgcacgaacg
      481 ggatcatgat ctcaacgttg gtcagcccca tgtcgttgcg cacacgtttc actgcttcac
      541 actccagcgc gaagcagtcg cggaagctgt cggaaacata gcggcccgcg ccacggaagc
      601 cgagcatcgg gttctcttca tctggctcgt aacgctcacc accgaccagg ttggcatatt
      661 cgttcgattt aaaatcagag agacggacaa tgacgcgctt cggataaaac gcggcaccca
      721 gcgtcgcgat cccttcagtc agacgaccaa cgtaaaattc acgcggagaa tcaaaacctt
      781 tcatcatctc gcggatttcg ttttgcaact gcggttcctg atcgtcaaac tcaagcagtg
      841 cgcgtgggtg gacgccaatc atacggttga tgataaattc cagacgcgca agtcccacgc
      901 cttcgttcgg cagacaggcg aagtcgaaag ctcgatccgg gttaccgacg ttcatcatca
      961 ctttcaacgg cagatccggc atggtttcta cgctggagct tttcacgcta aattccagca
     1021 actccgcata gacgtaaccg gtatcacctt cggcacaaga aacagtgacg ttctcaccgt
     1081 ctttcatccg ttctgttgca tcaccacagc ccacgaccgc cggaataccc agttcacgag
     1141 cgatgatcgc cgcgtgacag gtacgaccgc cacggttggt gactatggca gatgctttct
     1201 tcatgatcgg ttcccagtcc gggtcggtca tgtcagtgac cagcacgtca ccaggttcga
     1261 tgcggttcat ttcgctgatg tcatggatga ctttcaccgg acccgcaccg atgcgatgac
     1321 cgatagcacg gccttcggcg ataatcttac cctgtgaatg cagcgtataa cgctccatga
     1381 cctgaccgcg tgagcgcacg gtttccggac gcgcctgcac aatgaacagt ttgccggtgt
     1441 ggccatcttt cgcccactca atatccatcg ggcgaccgta gtgtttctca atttgtacgg
     1501 cctgttttgc cagttcctgc acttcttcgt tggtcagcga gaagatgtca cgctgttcct
     1561 gcggtacgtc ttcgatttta acctgcttgc cgtgctcctg ggtcggcgcg taaaccatgc
     1621 ggattttttt cgaccccatg gtgcggcgca cgatagccgg gcgattcgcc gccagtgtcg
     1681 gtttatgcac gtaaaactca tccgggttaa ccgccccctg cacgaccatc tcaccaaggc
     1741 cccatgcgga agtgataaac accacctggt caaagccgga ttcggtatca atggagaaca
     1801 tcacgccaga agatgcgagg tcggagcgca ccatccgctg aacaccggcg gagagcgcca
     1861 cgccacggtg atcgtaaccc tggtgcacac gataagagat ggcgcgaccg ttaaacagag
     1921 aagcaaatac atgtttcact gccacgagaa cggcgtcaaa cccctgaacg ttgaggaagg
     1981 tttcctgctg accggcaaag gaagcgtccg gcatatcttc tgcggtggcg gaggagcgca
     2041 ccgcaaaaga ggcgttttcg tcatcggcgg aaagctgagc ataggcttcg cggatggcgt
     2101 tttccagctc aggctggaag ggagtgtcga taatccactg gcggatttgc gcgcccgctt
     2161 tcgcaagctg agtaacatcg tcaatatccg ttttatccag cagttcataa atgcgctggt
     2221 ttacgccgct ttggtccaga aactggttaa acgcgtcggc ggttgtggcg aaaccattcg
     2281 gaacggaaac gcccattccg gaaaggttag taatcatttc acccagggag gcatttttgc
     2341 ccccaaccct gtctacatca ttcatgccga gttggttgta ccaaagcacc agcggtgacg
     2401 agccattgtt ggacatcgaa caatcctttt gtgataaatg aacggtttga gaaacacatt
     2461 tctgcgcatt tatctttgca tatttaaccg gatgaaaaaa acggtgaatc gttcaagcaa
     2521 atatattttt ttacttttt
//
LOCUS       DAJVOY010000292         2490 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVOY010000292 DAJVOY010000000
VERSION     DAJVOY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.295"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(152..571)
                     /locus_tag="OQL90_004082"
     CDS             complement(152..571)
                     /locus_tag="OQL90_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1370652.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            651..905
                     /locus_tag="OQL90_004083"
     CDS             651..905
                     /locus_tag="OQL90_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1370653.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            902..1327
                     /locus_tag="OQL90_004084"
     CDS             902..1327
                     /locus_tag="OQL90_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1370654.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            1399..2469
                     /locus_tag="OQL90_004085"
     CDS             1399..2469
                     /locus_tag="OQL90_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCS1370655.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQAMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
BASE COUNT          671 a          544 c          665 g          610 t
ORIGIN      
        1 gcattctgtg gggcggggtg ggtggcaggt atataatgta ctttgcgttc attgttgtaa
       61 agtgcttatg gtacattttg tgtgtaaaaa aatgagatgg gataaagtga agcacaaacc
      121 cggaggaagg cgctaccgga tttatgctgg tttaagaggc tttttgtttt ttctttcgtg
      181 ctaactcttc gtaaattgca ttgtacttct gttttttctc ttcaagagtt tttaaaagtt
      241 catctgtctc actgtcaggg agctcgtcca gaaggtcaat gatgattttt tgtcttggat
      301 ttaactcctg atagaaacgt acctgtccac tttcttctgt atcctctccc aaaagatagg
      361 ttggtgttgt tcctattagt gttgctaatt cccttaattt ctcccggcga ggaattgttt
      421 cgccattaaa ccatttgcta accgcttttg gtgttaattt cattcgacgg gcaatttctg
      481 cctgccttcc atgttgttca taaccagcgt tttcacaggc tagcgcaagc ctactggcga
      541 actctttacg cgctttatct tcatgaacca taagttcaat gatattcgct cttgaatgta
      601 ctgtcagttc tgttatagca tgtactcaaa gttcacattg tgagggtgat atgaaccaga
      661 aaacacttga agatgtaatc aaaactgttc gcgttgctgt tgtggccgac gtttgtggtg
      721 tcagccaaag agcaatctat aaatggatgg ataacggaaa attgcctcgc acagaatata
      781 ccggcgaaac aaattacgct gaaaaaatcg ctcttgcatc aaacggatta ttttctgccg
      841 atgcaatttt aactattggc aggaataaaa ctactacgaa aaagctgatg ggagttgatt
      901 catgaaaatc aagcatgaac acatccgcat ggcgatgaat gcctgggcgc atccggacgg
      961 cgaaaaagta ccggctgcga aaattaccaa agcgtatttc gagctgggaa tgacgttccc
     1021 ggaactgtat gacgacagcc atccggaagc cctggctcgc aatactcaga aaattttccg
     1081 ctgggtggag aaagacaccc ctgatgcgct taaaaaaatt caggcgttgt taccagctat
     1141 cgaaaaagca atgccacctc tgctggtggc ccgaatgcgc agccatagct cagcctattt
     1201 tcgggaacta gtggagacgc gggaacgact ggtgagagac gctgatgatt ttgtcgcagt
     1261 ggcgatcgct ggtttcaacc agatgaatcg tggtggccct gcaggaaata ttgtggctgt
     1321 gcattgactc gcaatattca taccggatca cttccggcaa tttgtgagta aaaagattcg
     1381 gtatcaaaag aggtgagtat ggctaacgcc tggctcagat tatggcatga catgccaaat
     1441 gaccctaagt ggcgaacaat tgccagggtg tcagggcagc caattgcaac agtgatggca
     1501 gtgtatatcc acctcctggt gagcgcgtca cgaaatgtca cgcgaggtca cattgatgtc
     1561 acgacagaag atttggcaag tgcgctcgac gtgacagaag aggtaattga ttcaattttg
     1621 cagacgatgc aggggcgggt acttgatggt gatttaatca ctggatggga aaaacgccag
     1681 gtgctgaaag aggacaacgg caatatttcg caaaccgcaa aatctcctgc agagcgcaag
     1741 agggcgcagc gagagaggga aagaaagcgg gaacaaaatg gcgattgtca cggcgcgtca
     1801 cgatatgtca cgcacatgtc acgacgagtc acgacagata aagatacaga taaagataca
     1861 gatcaagaag atcaaaacac tatggtccat ggcgtaaaaa acgccacgaa ccaggcaggg
     1921 gatgttcaga ccgtcaatcc tggtcagcca gcaggcacga caccggaagc cgattcagcg
     1981 tatgcgctga aagccgattc gggcgctgtg cagcaggcga tgaccgcaag tccggagcaa
     2041 tcacaccaac tgcagcagcc tgaagccgat tccgccattc agcgggaagc cgatcgggta
     2101 gtcccggaaa acaccgggca gtctgtggga cgagtggatt atccggatgt gttcgaacag
     2161 gtctggcggg agtacccgtt gcgtgccggg gcaaacccga agaaatccgc tttcagtgcc
     2221 tggaaggcca gattacgcga gggggtgcca ccagaggcca tgctggatgg cgtgaggcgt
     2281 tacgcaagat acctggcggc taccgggaaa acgggaacgg aatttgttca gcgagcgacg
     2341 acgttttttg gaccggaccg gaattttgag aacccctggt tgctcccggt aagcggcacg
     2401 aacaaccagc gttgtgtgaa tcatatttct gaaccggata acgaaattcc gccgggcttc
     2461 agggggtaag tgttaatttc tggtcatgag
//
LOCUS       DAJVOY010000293         2440 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVOY010000293 DAJVOY010000000
VERSION     DAJVOY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.296"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(80..871)
                     /gene="kdgR"
                     /locus_tag="OQL90_004086"
     CDS             complement(80..871)
                     /gene="kdgR"
                     /locus_tag="OQL90_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1370656.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            1048..2421
                     /locus_tag="OQL90_004087"
     CDS             1048..2421
                     /locus_tag="OQL90_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1370657.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT          517 a          645 c          571 g          707 t
ORIGIN      
        1 taacgcacct tcaactttat ttttctcatg ctaaataaag aacctgtctg tttgatattc
       61 cgacaggttc ttactctcat cagaacggat agtcgtgata acccatttgg gcagaaattt
      121 tgcgcgctgc ggtatgcaac attgcgacat attcctgtaa acgctcttca gagaaacgca
      181 acgtcgggaa ggagatgctc aaaccggcaa tgaccacgcc aaagcgatca aataccggta
      241 ccgcaatgca tcgcagccct tcttcctgct cttcgttatc ttcgccatac ccctgctcgc
      301 gcacctggtc cagaacgggt aataacgctt ctgtactggt gatggtccgc tcggtactgc
      361 gtttatactc cacgccctca agaatttgct tcacttcatc gcgatcgcgc catgccagca
      421 gtaccttacc aatcgcggtg ctgtacagcg gattacgacg cccaatccgt gaatacatgc
      481 gcaaattgta catagagtca attttgtgaa tgtaaacaat actgtcttcg tccagtgcgc
      541 cgaggtggat agtttctttg gtcaggcggg agatctcacg catctggata tctgcgctac
      601 gaattaaatc gacgttttgt aacgcgcgag cgcccagttc aaacaatttc agggtcaggg
      661 aatatttctc cgactcccct tcctgcgcca cataacctaa ggttttcatg gtctgtaaaa
      721 agcgataaac ggtgcttttt gacatcatga cgcgctgcga cagctcggtt atccctattt
      781 cgcgctcttc acccagcgcc tgcaaaatgc caaaaacttt tagcacggaa gatacagaat
      841 caggctgttt atccagatct gcgttagcca tttatcacct cattgcgagt gttttataaa
      901 aatcagaact gttttttatt ataatttcgc accagggggg tcgcaatcca tcttttgccg
      961 gttagttaca attctgcgac atccaccgtg aatatcagtg ctagaatcat acccctattg
     1021 attattcacc aaagatataa aattcctatg ccaaaagttc aggccgacgg cctgccattg
     1081 ccccagcgat acggtgcgat attaaccatt gtgattggta tttcgatggc tgtccttgac
     1141 ggcgcaatcg ccaacgtcgc cctgccaaca atcgccacgg accttcatgc cacgccagcc
     1201 agttccatct gggtagtgaa cgcctatcaa atcgccattg tcatctccct gctctcgttt
     1261 tcgtttctgg gcgatatgtt tggctatcga cgtatttata aatgcggtct ggtcgttttt
     1321 ctgttgtctt cactgttctg cgccctttct gattcgctgc aaatgctcac ccttgcgcgt
     1381 gtcatacaag gtttcggcgg tgcagcgttg atgagcgtta ataccgcact tatccgcctg
     1441 atctatccac aacgttttct ggggagaggg atgggcataa actcgtttat tgttgccgtc
     1501 tcttctgctg ccgggccgac aattgctgca gcaatcctct ccatcgcatc ctggaaatgg
     1561 ttatttttaa tcaacgtacc gttaggtatt atcgccctgc ttctggcgat gcgttttctg
     1621 ccacccaatg gttctcgcgc cagtaaaccc cgtttcgacc tgcccagcgc cgtgatgaac
     1681 gcgttaacct tcggcctgct tatcactgcg ttgagtggtt tcgctcaggg gcaatcgctg
     1741 acgttaattg ctgcggaact ggtggtaatg gttgttgttg gtattttctt tattcgccgc
     1801 cagctttctc ttcccgtacc gctgctaccg gtggatttac tgcgtatccc gctgttttca
     1861 ctttctattt gcacatctgt ttgctctttc tgcgcacaaa tgctggcaat ggtttccctg
     1921 cccttttacc tgcaaaccgt gctcgggcgt agtgaagtcg aaacaggttt acttctgaca
     1981 ccgtggccgt tagcaacgat ggtgatggct ccgttggcag gctatttgat tgaacgcgta
     2041 catgcaggat tgctgggtgc tctagggttg ttcatcatgg ctgcggggct tttttccctg
     2101 gttctgctgc ccgcgtcacc tgcggatatc aatattatct ggccgatgat cttatgtggc
     2161 gctggatttg gcttgttcca gtcacccaat aaccacacca ttattacctc cgctcctcgc
     2221 gaacgtagcg gtggagccag tggcatgtta ggaacggctc gtctactggg tcagagtagc
     2281 ggcgcggcac tggtggcgct gatgctaaat cagttcggtg ataatggtac gcacgtttcg
     2341 ctgatggctg cggctattct ggcagtgatt gctgcctgtg tcagtggttt acgtatcact
     2401 cagccacgat ccagggcata aaaaaagcgc gtcgatcagg
//
LOCUS       DAJVOY010000294         2324 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVOY010000294 DAJVOY010000000
VERSION     DAJVOY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.297"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            103..1239
                     /gene="potA"
                     /locus_tag="OQL90_004088"
     CDS             103..1239
                     /gene="potA"
                     /locus_tag="OQL90_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1370658.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            1223..2086
                     /gene="potB"
                     /locus_tag="OQL90_004089"
     CDS             1223..2086
                     /gene="potB"
                     /locus_tag="OQL90_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1370659.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
BASE COUNT          546 a          570 c          589 g          619 t
ORIGIN      
        1 ttggcgattc aagcggtttg ccgtagaatt accgccctca ttaagagtca ccaaggtggt
       61 taaccacaaa ccccgcatcg gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa
      121 ttgaataaac aaccgagttc gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc
      181 tttgatggta aagaggtcat tccccagctg gatctgacta tcaacaatgg cgagttcctc
      241 acgctgcttg gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg
      301 gaaactgttg attccggacg catcatgctg gataacgagg acatcaccca cgttccggcg
      361 gaaaaccgct atgtgaacac tgttttccaa agctacgcac ttttccccca catgaccgtg
      421 ttcgaaaatg tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga aattacgccc
      481 cgcgtgatgg aagccctgcg gatggtgcaa ctggaaacct tcgctcaacg taaaccgcat
      541 cagctctctg gtggtcaaca gcaacgcgtc gccattgctc gcgcggtggt taacaagcct
      601 cgtctgttgt tgctggatga gtcgctctca gcgctggatt acaaactgcg taagcaaatg
      661 cagaacgagc tgaaagcgtt acagcgtaag cttggcatta cattcgtctt cgtgactcac
      721 gaccaggaag aagcactcac catgtcagac aggattgtgg tgatgcgcga gggtcgcatt
      781 gagcaagacg gcacgccgcg tgaaatctac gaagagccga aaaacctgtt tgttgccggc
      841 ttcattggcg aaattaatat gtttaacgcc acggtgatcg agcgactcga cgagcagcgc
      901 gtacgcgcca acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg
      961 gggcaaaaac tgcatgttct gctgcgcccg gaagacttac gtgttgaaga gattaacgac
     1021 gacaaccacg ctgaagggct gattggttac gttcgtgagc gtaactacaa aggcatgacg
     1081 ctggagtcgg ttgttgaact ggaaaatggc aagatggtga tggtcagcga attcttcaat
     1141 gaagacgatc ctgactttga ccactctctc gaccaaaaaa tggccattaa ttgggtagaa
     1201 agctgggagg tcgtactggc tgatgaagaa cacaagtaag ttccagaatg tagtgattgt
     1261 cactattgtc ggttggcttg tgttgtttgt ctttctgccc aacctgatga tcattggcac
     1321 cagctttttg acccgcgacg acgccagttt cgtcaaaatg gtctttacgc tggataacta
     1381 cacgcgtctg ctcgatccgc tctattttga agtgctattg cactcgctga atatggcgct
     1441 gatcgccacc ctcgcctgcc tggtgctggg ctatccattt gcctggtttc tggcgaagct
     1501 gccacacaag gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct ggaccaactc
     1561 attgattcgt atctacgggc tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt
     1621 tttgctctgg ctgggcgtta tcgacacacc aatccgtatc atgttcacgc ccagtgcggt
     1681 gattatcggt ctggtttaca ttctgctgcc gtttatggtg atgccgctgt actccagtat
     1741 cgaaaaactg gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca
     1801 gacttttatc cgcatcatta ttccactgac gatgccgggg attattgccg gatgtctgct
     1861 ggtgatgctg ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa
     1921 cctgctgatc ggtaacgtca tcaaggtaca gttcctcaat attcgtgact ggccgtttgg
     1981 tgcagccacc agcattacgc tgactatcgt aatgggcctg atgttgctgg tttactggcg
     2041 cgcttctcgt ttgctgaata agaaggtgag cgaattagat gattaatcat ttatattcaa
     2101 tgcattaagt ttgtttttga gtggttttgc cgtttaaggt aacggcgtta tagatcctat
     2161 caaaatcaat caattccatt ctggttttgg agaaagattt ttcgagagct tcggtcttta
     2221 actaaaactg attgcgtata accccaatac ccctccccga ctatctgaat ggtcggggag
     2281 gcacttcacg cacaaaatat catccctgcg ccgttgatta ctct
//
LOCUS       DAJVOY010000295         2315 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVOY010000295 DAJVOY010000000
VERSION     DAJVOY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.298"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(46..382)
                     /locus_tag="OQL90_004090"
                     /pseudo
     CDS             complement(46..382)
                     /locus_tag="OQL90_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(915..1241)
                     /locus_tag="OQL90_004091"
     CDS             complement(915..1241)
                     /locus_tag="OQL90_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370660.1"
                     /translation="MFKYAEMLMAEEDNKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLILCALGVNSWRGKSKYLDYYLLRDEMPVRLVSIIFPTLL
                     ALFLLA"
BASE COUNT          761 a          447 c          402 g          705 t
ORIGIN      
        1 aaatatgaaa agatggttta aataccccgt tacttttgac ttacactata cggtatcgca
       61 tcgtttaata ttcgcaccgg ccagattttt atttctatta gttgtcacaa tactgaatgc
      121 gtacgaccac agtattctgg ctcctgtgtg gtcatgcttt aattctgcgt tccggacaga
      181 taagcagttg cttgcaggaa tccttcttgt gttaatgtca gttccccttt ttaccagtgc
      241 tgatttccac attccgtcca acagagctta tagcctttcc ctggattata gcattgtccg
      301 gctgaagttc tttttgaata ataatagaag cactgctggc agatccagtc cgtttttcat
      361 aacccactgt actgataacc ataatctaat cagtagaaat tgagtcgaaa ataagcacta
      421 ctccatacag gataattaga ggtcagtttg attattcaca attcattatc agcattttct
      481 gtttctgacg aaatcaacat gaaaagaacc atatatgata attattataa taacggcttt
      541 aattggaata catatattac aacgtattat atataattgg tattctggga actatattct
      601 caaaatacag tagaaacgag gtatgtttct ggtggaaagg acagtgggat taaaaagtaa
      661 gaattgataa aaaaacgcca gcaacacata gtgccggcgg agggaatccc ccatggagaa
      721 aatgtgatgc ctagagcacc atgaatatat aattaaaaat agttagagtt gtcaatactt
      781 tcacaaaaat aattttcgta gtttcgtagt atagaaagat atttttatgc atgacctacc
      841 tgaatttgct ccgggtagag gttataaata aaaattgaat cacgacaaac acaatattca
      901 tagtatggcg atgcctacgc cagcaagaat agcgccaata atgttgggaa tataatagac
      961 accagacgca caggcatctc atctcttaac aaataataat caaggtattt acttttgcct
     1021 cgccagctgt ttacgcctaa agcacacaag attaaccatt caataacaaa tggcaatatc
     1081 catagccata cgactttacc ttgtaatgtt cggtatttct ttataattat tctgggaaat
     1141 ctatcattta tttttaaaat caaattatct tgttgtttaa aaataagttc acatacttta
     1201 ttatcttctt ccgccatcaa catctctgca tacttaaaca tttcagaacg ttcctttagc
     1261 acagaagagt aattatatgt ccagttccca ggcaataatg cttatggata tttaactaat
     1321 aatttagaga atattttgca ataatatttg gctgtattca gaaatacctg aaaaatcata
     1381 ctatacagcc ccagggaatg gataaagatt ctaacaaagc atttcaacaa tatatgcttg
     1441 ttaaaaatcc atatcgaaat gccgttgcag cattaatata tgcctcattc ataaaatgta
     1501 aaagagcaag ctcgtaccag taggggggaa attcattgac atgtcctgtc aatacgtaca
     1561 gagccctgtc atgcttgcca gcacgtaaca attccgtccc cagtaaccag cgtgtcccct
     1621 gattattctc agggttatat gttagtattt tatcaatgag tataacggca tcctgatgac
     1681 gctgtaaatg aacattcgcc agaattgcag cataaagagc gcaaatgcat atgccagatg
     1741 agcgtgaata tcaatgatgt caggagcatg tcaggcaaga cgcctgagtt tgttgacgta
     1801 atttgtttcg tttatttcat cactatcgta tgcgtcaaga gttttttcaa actcatcccg
     1861 aaatgggcca agcctggttt caggaaataa gaaatacccc tagttattgt tcacttaaaa
     1921 gtataatttg taattcaaat cctgagcctg taaaacgggg agtaacatat tctattctga
     1981 agagaataaa agtcgtgatg cgatgtatca agcccggatt gtaatcccag attaacatag
     2041 atcacaaaac aacttattct tcactaacgt gaagataaat atcgttgtat gccttatcac
     2101 gactacgaat accagcaaga tacaactgac atacggaaag ataccatgtt ttttactccc
     2161 gaaaataacg ctaaaaagct acttccccat cgtttgtcct tagtattgtc agcgccaaca
     2221 atgtgggctg acatgataaa gctgtctagg aaaacgttcg cctcctcagc ggacaatcca
     2281 aatggtgatt gtccctatta aacgtttatt ttgaa
//
LOCUS       DAJVOY010000296         2302 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVOY010000296 DAJVOY010000000
VERSION     DAJVOY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.299"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OQL90_004092"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OQL90_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1370661.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OQL90_004093"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OQL90_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCS1370662.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OQL90_004094"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OQL90_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCS1370663.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          618 a          490 c          541 g          653 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agcaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaggg caacagatcg cgtacccagt
     1021 ttgccggcca gtcttggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattca ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatacgataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aaccacggca gcctggcggc
     2161 tgcaaatcgg ggtgtggcag aatatgagct ttcagagtaa ccatccagaa tgagttccca
     2221 tgtcacacaa cctggagcac cagaaagtcc acaccagaat ggtgaaagag gttctgaaag
     2281 ccgtcgccag agccaataat ca
//
LOCUS       DAJVOY010000297         2289 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVOY010000297 DAJVOY010000000
VERSION     DAJVOY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.301"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..184
                     /locus_tag="OQL90_004095"
     CDS             <1..184
                     /locus_tag="OQL90_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1370664.1"
                     /translation="WSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLN
                     RRLSESVPCMPHKKAG"
     gene            205..906
                     /gene="mlrA"
                     /locus_tag="OQL90_004096"
     CDS             205..906
                     /gene="mlrA"
                     /locus_tag="OQL90_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCS1370665.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDDGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            966..1073
                     /locus_tag="OQL90_004097"
     CDS             966..1073
                     /locus_tag="OQL90_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1370666.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1054..1785)
                     /gene="yehW"
                     /locus_tag="OQL90_004098"
     CDS             complement(1054..1785)
                     /gene="yehW"
                     /locus_tag="OQL90_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1370667.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1790..>2289)
                     /gene="yehX"
                     /locus_tag="OQL90_004099"
     CDS             complement(1790..>2289)
                     /gene="yehX"
                     /locus_tag="OQL90_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1370668.1"
                     /translation="GVARALAADPQVLLMDEPFGALDPVTRGALQQEMTRIHRLLGRT
                     IVLVTHDIDEALRLAEHLVLMDHGEVVQQGNPLTMLTRPANDFVRQFFGRSELGVRLL
                     SLRSVADYVCREERAEGEALAEEMTLRDALSLFVARGCEVLPVVNTQGQPCGTLHFQD
                     LLEEA"
BASE COUNT          590 a          672 c          569 g          458 t
ORIGIN      
        1 ctggtcactt gccgcaggag ctaatcctag ttttatcgca agccagatgg gccacacaaa
       61 cgcacaaatg gtattcaatg tttacggagc atggatgaaa gacaacaatc acgaacagat
      121 agaacttctt aacagaagac tatctgaaag tgtcccatgc atgccccata agaaagcggg
      181 gtaaaataaa aacttgcaaa atcaattggt ttacctttaa tccctgtcac gttacgcgcg
      241 tggcagaggc gttacgggtt gctgaaaccg caacggacag acgacggtca tcgactgttc
      301 aacgatgccg atattgaccg gatccgcgag atcaaacgct ggatcgacaa cggcgtgcag
      361 gtcagcaaag ttaaaatgct gctcagtaat gaaaatgttg atgtgcagaa cggctggcgc
      421 gatcagcaag aaacattact gacctacctg caaagcggca atctgcatag cctgcgaacg
      481 tggatcaaag agcgcggtca ggattacccc gcccagacac tcaccacaca tctgtttatt
      541 cctctgcgcc gacggcttca gtgccaacaa ccgactctcc aggcgctgct ggcgatcctc
      601 gacggcgtac tgatcaacta catcgccatt tgtctggctt cggcacgtag aaaacagggt
      661 aaagatgcgc tggtggttgg ctggaatatt caggatacca cccgtctgtg gctggagggc
      721 tggattgcca gtcaacaagg atggcgcatt gatgtcctcg cccactcgct caatcaacta
      781 cgccctgaac tattcgaagg ccgtacattg ctggtgtggt gcggtgaaaa tcgaacctcc
      841 gcccaacagc agcaactcac cagttggcaa gaacaaggcc atgatatttt cccactcggc
      901 atttaatgat tcgttaacaa atgcgcttta ctgtacaatc ctttcgttaa cataaggagt
      961 gcaatatgcg catagctaaa attggggtca tcgccctgtt cctgtttatg gcgttaggcg
     1021 gaattggtgg cgtcatgctc gcaggttata cctttatttt gcgtgctggc taagcgcctg
     1081 caccagcctt tcaaacaggc ggtctgcaat gatcgccgcc agtgccacca gtaacgcccc
     1141 ctggatcaca tacgcggtat taaatccgct aagcccgatg atgatgggcg tacccagcgt
     1201 gctggcccct accgttgagg cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg
     1261 cacgcccgcc agaatcaccg gagccgccag cggtagctcg accttacgca gtcgctgacc
     1321 acgactcatt cccatacctt tcgcaacttc tgtcacgctg gcatcaatcg ctcccagccc
     1381 ggcaagtgtc gcctgcagga cgggcagcac accgtaaagg atcaaggcga taatcgctgg
     1441 ttgcagacca aagccgatca ccggaacggc aatcgccagc actgcgacag gcggaaaagt
     1501 ctgtccaacg gcggcaatag tttccaccag tgggcgaaat tccgcgcccc acgggcgagt
     1561 gacagcaatt ccggcaccag tgccaatgat caccacaaac aaactcgaaa ttcccaccag
     1621 ccagaaatga gccagtgcca gagctgcaaa actttcttgc tgataaacgg gtcgtggcag
     1681 ttgtgggaac aaggcagcaa acagcggctg gctgtaaggc agccagaaaa tcagtgccac
     1741 aaacaaagca atgagccaga acagcggatc gcgcaacatc ttcatacgct tacgcctcct
     1801 ccagcagatc ctgaaaatgc agcgtgccgc aaggctggcc ctgcgtgttc accaccggca
     1861 gcacctcgca tccccgcgcg acaaacaggg agagcgcatc gcgtagcgtc atctcttctg
     1921 ccagtgcctc accttctgcc cgttcttcgc gacacacgta atccgccaca ctacgtaacg
     1981 aaagcaggcg cacacccagt tcgctgcgcc caaaaaactg gcggacaaaa tcgtttgccg
     2041 gacgagtcag catcgtcagc ggattgccct gctgcaccac ttcaccgtga tccatcaata
     2101 ccagatgttc tgccagccgt agcgcctcat caatatcatg agtgaccagc acaatggtac
     2161 gccccagcaa acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt
     2221 ccagtgcgcc aaaaggttca tccatcagta agacttgcgg atcggcagcc agtgcgcgcg
     2281 ccactccca
//
LOCUS       DAJVOY010000298         2272 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVOY010000298 DAJVOY010000000
VERSION     DAJVOY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.302"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..346)
                     /locus_tag="OQL90_004100"
     CDS             complement(<1..346)
                     /locus_tag="OQL90_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCS1370669.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTR"
     gene            588..881
                     /locus_tag="OQL90_004101"
     CDS             588..881
                     /locus_tag="OQL90_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1370670.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            953..1612
                     /locus_tag="OQL90_004102"
     CDS             953..1612
                     /locus_tag="OQL90_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1370671.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1704..2150
                     /locus_tag="OQL90_004103"
     CDS             1704..2150
                     /locus_tag="OQL90_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1370672.1"
                     /translation="MNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          594 a          489 c          584 g          605 t
ORIGIN      
        1 ttcgggtttg cagcactcgc agctcatatt tgccagaagc agggagtgag tattgtccgt
       61 gactatcgag ttctggggaa tatctggata aatcaacaga atcatcgatt cctggcccga
      121 agaggtaagt atcagcgcct tcattagaga tactcacatg tactttctga ccttttttgg
      181 catagaaggt atatgtatcg taatcgtatc cctttatttc gccggagtac tgagcgctgc
      241 tatggccttt tctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
      301 gcagtgcaaa cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      361 tcagaatgag tacgatgaac tgttataata taacaatccc taacgggaga attccagtgg
      421 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggtgggat tacttataaa
      481 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      541 attgatgtta gcttatgcca aaacctggca tcttaaaaag taaaagtatg ttttgtgtga
      601 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      661 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      721 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      781 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      841 tttccgtcat ggggcagaaa acagacgaca cgaacaaata accgatatcc ggcggtggca
      901 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      961 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
     1021 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
     1081 gtttattaaa ccagaaacgg cactgtgcga tctgaggcag ccattggcga tcccatccga
     1141 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggtgcga cgctgcgtca
     1201 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1261 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1321 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1381 acaaaataca acgctgggcc tgagcgtaaa cggcgaacaa cgccagcaag gtacgactgc
     1441 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1501 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1561 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1621 gccgcctgaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1681 ttaacgtaat ggcggaacac ctgatgaacg atgtaccttt ctggcaaagt aaaaccctgg
     1741 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcctgc
     1801 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1861 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1921 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1981 atcgtttgct ggcggaaggt aaagatttac ctgtgtggca tccgctactt actggttcga
     2041 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     2101 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     2161 agatgttgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     2221 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac ag
//
LOCUS       DAJVOY010000299         2255 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVOY010000299 DAJVOY010000000
VERSION     DAJVOY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.303"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..188)
                     /locus_tag="OQL90_004104"
     CDS             complement(<1..188)
                     /locus_tag="OQL90_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370673.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEG"
     gene            complement(198..1580)
                     /locus_tag="OQL90_004105"
     CDS             complement(198..1580)
                     /locus_tag="OQL90_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCS1370674.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1873..1967
                     /locus_tag="OQL90_004106"
     tRNA            1873..1967
                     /locus_tag="OQL90_004106"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1907..1909,aa:Sec,seq:tca)
     gene            2046..2210
                     /locus_tag="OQL90_004107"
                     /pseudo
     CDS             2046..2210
                     /locus_tag="OQL90_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280578.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
BASE COUNT          605 a          565 c          534 g          551 t
ORIGIN      
        1 ccttcctgtg gggagaaaaa gcgcagcgta aataaaggcg tatcaagctg ccaggtacgt
       61 tcacgcacat cacggggggc agcatagacc accatttcat tatcctgctg ttcaacctcg
      121 aacacctgaa gcgggtgaat caaattgagg ccaggttgaa tcaaccagtt tccatcgcta
      181 attttcattg ccgttcctta gttctgcact tcttgagagg tgaattgttg ctggcaacgg
      241 cgtttaccct gagccagctg ttccataacg gttttcagat tgtgcgtcgt gagtgagtag
      301 tagcgtttag cgataatcgc gctcagcaaa taacagatcg ccggaacaat cgtgaatagc
      361 gcaataatga tgctaatcgt ggcgctgttc tgcgcttttt ctgccgcatc atatccgcca
      421 taagccagca tccagccgat aagagcgccg ccgaaggcca accccagttt gagcacaaac
      481 agcgtgccag caaaactgat cccggtcagg cgtttaccat tgcaccactc gccgtagtcg
      541 acggtatcgg acatcattac ccactggata ggtgtcacca gttgatgcaa cacaccaatc
      601 acgaagatga agacaaacat agtgatgctg gcctgcatgg gaacaaagaa catcgcgagg
      661 ctaatcactg ccagcagggc gttcgtccac cagaagatag tgactttaca tttccagtcg
      721 gtcagaggtt ttgccagtgc ggaaccaatc aggttaccca cgcaataagt ggtgagaaaa
      781 gcgacaaaca cttccggcgt gcccaaaatc catgtgacgt aatacatcat cgccccaccg
      841 agtacgcaca ccgccaggat attgaaaatg gttagtaaac cgacaatccg ccactggtcg
      901 ttttgccaga tatcacgtaa atcttcccgc atagacgttg ttgtaggtgg tgcttcaacg
      961 cgttctttag tggtgaagaa acaaaatgcc agcatcatga atgccaccac ggaaaggacc
     1021 gcgataccgc cctggaaacc gagtggttta ttatcaccgc caattaaatt aaccagtggc
     1081 atcatcagaa cagtagaaag catgcctccc gcggtcgcca gcacaaaacg ccaggattgc
     1141 agcgagatac gctgagtcgg gtcattggtg attacaccac ccaatgcgca gtaagggata
     1201 ttgacgacgg tataaagtaa ggtaagtagg gtgtaagtaa ttgctgcata gatcattttg
     1261 ccgttcatac tgagatctgg cgtgctatag gccagtacac agacgatccc gaatggcagt
     1321 gcgccaaaca gtacccacgg acgaaattta ccccagcgag agcgcgttcg atcggccaac
     1381 aaccccatgc aaggatcgga aatcgcatcc agtgcacgag cgaccaaaaa catggttccg
     1441 acaaaaccgg caggaatgcc aaaaatatcg gtataaaaga acatcatata taacattacg
     1501 ttatcgaaaa taatgtggct ggcggcgtct cccatgccat aaccaatttt ctctttaacg
     1561 gacaacactt cactcttcat ctgcttttcc ttgatgctat aggggtgctg agaccgataa
     1621 cgcattttgt aaaccatccc tgtgagagcg aatattccga tatctggtta ttaatttatg
     1681 attcttgttt tatgtgatcg tggtagcgtt aattccgctc atatgtcatt gtaaaatatg
     1741 ggttttatat gaactataat gctttcgtga taatacgctg cgtgtattag gcggaaaaaa
     1801 ctgatctggg ggatgtagaa actcaaggaa gtagctataa tgcgccccgc ctccatgtag
     1861 caatcgaggc gcggaagatc gtcgtctccg gtgaggcggc tggacttcaa atccagttgg
     1921 ggccgccagc ggtcccggac aggttcgact cctgtgatct tccgccaaaa tgcctcttct
     1981 gacgttctcc aaccccttgt aatactgtca atccagatat tttctactgc tcaatattca
     2041 atcggcttca ggaatacctg gtctaaggtt ttgtgatgga cgatgagcgg ctgaagaatc
     2101 cgcctgtcgg ttcttctgtc ctcgaacgca tccgcgatat gcgcgccagc atgtgccgtg
     2161 tttatttgca ggtgatttgc cccctgctgg ctctcagcca actgtatcgc cattaaggtg
     2221 aagatgttgt tttacacgct ccataacacc ctttt
//
LOCUS       DAJVOY010000300         2252 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVOY010000300 DAJVOY010000000
VERSION     DAJVOY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.304"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..423
                     /gene="yeeE"
                     /locus_tag="OQL90_004108"
     CDS             <1..423
                     /gene="yeeE"
                     /locus_tag="OQL90_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1370675.1"
                     /translation="LAWPLSEATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVL
                     GIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMM
                     TWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            437..664
                     /locus_tag="OQL90_004109"
     CDS             437..664
                     /locus_tag="OQL90_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1370676.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(707..2131)
                     /gene="sbcB"
                     /locus_tag="OQL90_004110"
     CDS             complement(707..2131)
                     /gene="sbcB"
                     /locus_tag="OQL90_004110"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1370677.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWSGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          536 a          544 c          586 g          586 t
ORIGIN      
        1 ttagcctggc ccctgagtga agcaaccgga cgcatgtttg ggttaggaat cacttcccca
       61 acggccaata ttctgcaatt tctggtcgcg ggtgacgtga aatacattaa ctggggcgtt
      121 ttcctggtgt tagggatctt cgtggggtca tttattgcag ccaaagcgag ccgtgagttc
      181 cgcgttcgcg cagctgatgc acaaacaaca ttacgtagcg ggttaggtgg tgtactgatg
      241 ggcttcggtg ccagtattgc aggtggttgc tctatcggta atggactggt tatgactgca
      301 atgatgacct ggcagggctg gattggcctt gtatttatga ttctcggagt ctggactgca
      361 tcctggcttg tgtatgttcg accgcagcgt aaggcgcgac tggcgactgc tgcggcaaat
      421 taagtaaggt gtaaagatgg cgattaaaaa gctggatgtg gtgacgcagg tctgtccgtt
      481 tccactcata gaagcaaaag cagcactggc agaaatggcc agcggcgatg agttggtaat
      541 tgagtttgat tgcacccagg caactgaagc aatcccacag tgggcggcag aagaaggaca
      601 tgccatcacc gattatcagc agattggcga tgcagcgtgg agcatcaccg ttcaaaaagc
      661 ctgactcaac attgtcctcc gccgtaccag cggcggaggc ttcaaattag acgatctctt
      721 ccgcgtactg ccaaagtgct tttaacagcg ccactttttc tttgtcatcg gcatattgtt
      781 gtaccagcat ctgcaattca tcagcataac cctgcaaaaa ctctggcgtg aatacctggc
      841 ggcggtgctc cagccagcgt tgctgctcgg cataatccag cgtccccggg aagttgcgtg
      901 cccgataatt gaacaacagc ttttcaatcc gtttatcaac aaaagtgata tccagtgccg
      961 gtaaattacg tggctcggtt tccagcacaa ttttcattgc tgcacgatct gcgtcactga
     1021 aaaagccgtt ataaagctgt gcatccacgt tatctgtagg cgtaaacggt tcggcttccg
     1081 cgaatatcgc caccactttt tcacgcactt gcggattttc acgcagaatt ttcaggttat
     1141 cgaggcaatg ctgacgatta attcccagtc ggtcggcatc ttccgggcgt agcgtattcg
     1201 cctgggccag caccggacat ttattgatgt gcaccagctt aaccggaacg gcggcgttat
     1261 cgccaagatc ggtttttgcg gtatataaac gctcgcgcaa tgtgtcgcta tccagttcca
     1321 gtaatggcga aatgtctcct gccaaatcca ccataattac agcattgcga ttttcaggat
     1381 gccacgccag cggtgccacc cagctggtat tgccgctcca tgccccaaac attccggaaa
     1441 cgtgaaccag ggggggcatc tgcggaacat caatcaacgc catcagtttg tgtttattac
     1501 gatgggtaaa gagataatca aacaggcgtg gctgacgcgt ttttaccagt ttcgccatcg
     1561 caatagtggc gtacacatca gccatcgcat cgtgggcgtt gctatgttca ataccattcg
     1621 ctttggttaa atgctcaagg cgaaagctcg gtagaccgtc atcattttca ggccagttta
     1681 ttccttccgg gcgcagggca taacaggcac gcataacatc caacaaatcc cagcgcgagt
     1741 tatcatgctg ccagctccag gcgtaaggat cgtagaaatt acgataaaaa acgttgcgtg
     1801 tgacttcgtc gtcgaaacgc acattgttgt agcccagaat acaggtcttc ggtacggtaa
     1861 aaagcgagtg aatacgggcg gcaaacgcgg cttcgttttc tcctttcgcc cgtgcttcct
     1921 gcggggtaat accggtaatt aatactgctc caggctgggg taaatagtca tccgcgggct
     1981 tgcagtaaaa gacttcgggt tcgccgatga cattgaattc gctatcggtg cgaatggctg
     2041 cgaactgtgc agggcgatct aacgcggggt gcgtgccaaa ggtttcgtaa tcgtgaaaga
     2101 ggaaggtagg ttgtttatct gtgtctgtca ttgtctagca ttgtccactg tagttaatta
     2161 actgtctgtt ataactcgaa aaacaaccat tttttcacct gagtatgtct acgaacgtca
     2221 aatatagttg tttactgtgc gctgttgtcc at
//
LOCUS       DAJVOY010000301         2234 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVOY010000301 DAJVOY010000000
VERSION     DAJVOY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.305"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(25..>2234)
                     /gene="pdeF"
                     /locus_tag="OQL90_004111"
     CDS             complement(25..>2234)
                     /gene="pdeF"
                     /locus_tag="OQL90_004111"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1370678.1"
                     /translation="KLNATYIKIRDKWWGLPLFLPFLILPIFAHINTFAHISSGEVFL
                     FYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIIIIIIII
                     LCWGGYRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENL
                     VGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTK
                     KEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWA
                     VVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRL
                     AYVDPVVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWL
                     SPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSY
                     CYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQA
                     LEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWV
                     IEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTE
                     SNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIV
                     SNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKP"
BASE COUNT          673 a          530 c          499 g          532 t
ORIGIN      
        1 ttcattcaac gtatcaatta acggctacgg cttaccaata agataaccct gcatataatc
       61 gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact ctgccactac
      121 ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat aatccagact
      181 atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg cattcacatt
      241 tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
      301 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
      361 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
      421 gacttcaaaa ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
      481 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
      541 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc tatcggggct
      601 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
      661 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
      721 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcgacg
      781 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat ttagctctcc
      841 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
      901 cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc tatccagtgc
      961 ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc gcgagatcgt tacccgaaag
     1021 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
     1081 gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc cagggatgcg
     1141 taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg cgcgaacatt
     1201 tggcagatga accaccgggt ccacatacgc aagccgctga atgcgtctga ctaccgctct
     1261 ctgacgggtt gccagtacag ccatataatt gacaataaaa gagaatacca gataactgga
     1321 ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat tttgatagct
     1381 gtggatgctg atcatcaaca cgaccgccca gagcagagaa atcagcttat aaccatagcg
     1441 catcgctccc cacatcatca ggggcagcaa taatgacaag gtataattgg tgctaaaaat
     1501 tgtgcttttt tcatttaacg gcatgcataa cagcaacaat aaagcaccta atgccagtag
     1561 ccagagcgcg aactcttttt tggtgacttt ggcatcaacc tgctgtttta attgcgaata
     1621 atagctacgt aaataaaatg gatttcgcac tacccgaatg atgaagtagc acagcgggac
     1681 accgatcaga ttacccacca gtaaggcctg ataattgatt aaagtcccga ggttaaaagg
     1741 catgacaccg accagatttt ctctgctcgc caataatcct acaaatgcag caaactggaa
     1801 aagtatcaga aacagcgttg caggaaacac aatctgccag aatatacgct gggaaattaa
     1861 acgggtatca ccatgtgaaa cgttgttacg ccggggagta aagaccctgt aaccgcccca
     1921 acagagaatg ataatgataa tgataatgat aatgataata aaattagccg ttaatgatag
     1981 cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa atccctaagg cgatccctgg
     2041 caatgccgcc cagctgaaaa acatcatcat gctgatcatc agtgccagag gcagataaaa
     2101 gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta atgtgggcga aaatgggcaa
     2161 gattaaaaaa ggcaggaaca gcggaagccc ccaccattta tcacgtattt ttatataagt
     2221 tgcattcagt ttca
//
LOCUS       DAJVOY010000302         2223 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVOY010000302 DAJVOY010000000
VERSION     DAJVOY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.306"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            420..563
                     /locus_tag="OQL90_004112"
                     /pseudo
     CDS             420..563
                     /locus_tag="OQL90_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            710..978
                     /locus_tag="OQL90_004113"
                     /pseudo
     CDS             710..978
                     /locus_tag="OQL90_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1375..>2223
                     /locus_tag="OQL90_004114"
     CDS             1375..>2223
                     /locus_tag="OQL90_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1370679.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          747 a          473 c          375 g          628 t
ORIGIN      
        1 aaaaagaaca aatggtggtg tccgctattg ccagtacacc tcaagcttct taccatattt
       61 gaaataaagc tcaatagcgc aaattttttg ttcttcagta aacatgtttc atcttctgag
      121 agtccaggaa atcgtccgca tccccattac cgtcttatat cactggcgct gacatgtgct
      181 cattgatgaa gtgaatctga atgacatgtt ttgccatatc aacgccaacc ggtgtatatt
      241 tcatttgtgg agtctccagt ctgaggacgc tttatgcatc ccgtattggg cagtatgatg
      301 ccggaaagct gcgaggctcc acacctgcca gtttcaccct tttaaccatc atactactcc
      361 gtgtttaatt gtgtgggatg cgttcattac attttaacag tgtcaccccg tcggccacga
      421 aagataaaca ggtgaccaga aaagggatct tcatccagca catgctatat cttgaacgta
      481 acaacctgac cctccgcacc cgcatcaagc ggctggcacg taaaacaatc tgcttctcgc
      541 gttccgtgga aatccacgaa aagtcatcgg ctccttcatt gaaaaacaca tattctactg
      601 attggaagcg ccacccgaaa aagtatcgtt tttttacagt aaacttcatt tagccgacta
      661 taatgtaaaa aaataaattt tttaccgaaa tataacataa ggaagtaata tgcttacaca
      721 aactatattc ccttgtcttc ccaaaaaaca agaaaatatc atccctgaag tatcaaatac
      781 tatattagta tcttctactg ttactacaga tggatgtact gtttttaata aacaaaaaca
      841 gccatatacg aattgcaaat acctgctacc aaccgaacga aaaagttgaa attcacagct
      901 acagaaatac aatggttgac aaagataaat gaagccggaa ttgatgaaaa acaaagccaa
      961 cggtattctg atttctgaag ttaatttcag actcgtttaa gctccatttt caaaaaaaaa
     1021 aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaaaat agagcagtaa
     1081 ttctatttca gagaggagta actccatcta tcctcgatac gtactgaacg ttatcgtgat
     1141 atattcatca tgctttcttc agcattattc gagtacaggg agagacgcaa tgaccttaaa
     1201 taccttttta tggctgaatt accattctca ggaataacag cagactcctt cccatagacg
     1261 gcttaggggg aaagcaatac ccggagaaag ggtacaaagc aaactgtttt aaaattaata
     1321 agctatattc tatttcacta gaattcttat cgctgataag aaattttagt tataatgatt
     1381 aaatcaacca atatacaggc aatcggttct ggtattatgc atcaaataaa caatatatac
     1441 tcgttaactc catttccttt acctatggaa ctgactccat cttgtaatga attttattta
     1501 aaagcctgga gtgaatggga aaagaacggt accccaggcg agcaacgcaa tatcgccttc
     1561 aataggctga aaatatgttt acaaaatcaa gaggcagaat taaatttatc tgagttagat
     1621 ttaaaaacat taccagattt accgcctcag ataacaacac tggaaataag aaaaaaccta
     1681 ttaacacatc tccctgattt accaccaatg cttaaggtaa tacatgctca atttaatcaa
     1741 ctggaaagct tacctgcctt acccgagacg ttagaagagc ttaatgcggg tgataacaag
     1801 ataaaagaat taccatttct tcctgaaaat ctaactcatt tacgggttca taataaccga
     1861 ttgcatattc tgccactatt gccaccggaa ctaaaattac tggtagtttc tggaaacaga
     1921 ttagacagca ttcccccctt tccagataag cttgaagggc tggctatggc taataatttt
     1981 atagaacaac taccggaatt accttttagt atgaacaggg ctgtgctaat gaataataat
     2041 ctgacaacac ttccggaaag tgtcctgaga ttagctcaga atgccttcgt aaatgttgca
     2101 ggtaatccac tgtctggcca taccatgcgt acactacaac aaataaccac cggaccagat
     2161 tattctggtc ctcgaatatt tttctctatg ggaaattctg ccacaatttc cgctccagaa
     2221 cac
//
LOCUS       DAJVOY010000303         2172 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVOY010000303 DAJVOY010000000
VERSION     DAJVOY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.307"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            45..323
                     /locus_tag="OQL90_004115"
     CDS             45..323
                     /locus_tag="OQL90_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1370680.1"
                     /translation="MSDAVYRRLILMKAMSNITDCSVPDINRMLRFMFGKKRRAYVLN
                     NGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            310..840
                     /locus_tag="OQL90_004116"
                     /pseudo
     CDS             310..840
                     /locus_tag="OQL90_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1174..1695
                     /locus_tag="OQL90_004117"
     CDS             1174..1695
                     /locus_tag="OQL90_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370681.1"
                     /translation="MDVYANSQRVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRSVVAELGDDSGWHFFSQRNTDNSITFAVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYGHEMYYSPGSNTVSWRFRAPSGHGLSGIAISDTGRDSADNVNGVYYRPLQ
                     KLINGTWYNVASV"
     gene            1705..>2172
                     /locus_tag="OQL90_004118"
     CDS             1705..>2172
                     /locus_tag="OQL90_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1370682.1"
                     /translation="MLKNITAGNPKTVEQYQLTKQYDVTWLFSKDGKNWYEELKNFAS
                     DTIKITYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFD
                     YRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMD"
BASE COUNT          570 a          480 c          599 g          523 t
ORIGIN      
        1 aggcaccgtt ttacaacgga aaatcggata cccggactgt tgacctgtct gatgctgtat
       61 accggcggct gatactgatg aaagccatgt cgaacattac tgactgttcc gttccggata
      121 ttaaccggat gctgagattt atgttcggaa aaaaacggcg agcttatgtt ctgaataatg
      181 gtgggctgag gatgagttac atctttgagt ccgcgctctc gttggcagaa ctggcgatta
      241 tccagtcgtc gggtgcactg ccgtccccgc cgggtgttta tgtttcagta gttttaaagg
      301 agtcccgtaa tgaaggccag tgataaaccc cgccagctgg cggtcccctt tgcgagtacc
      361 ggagataaaa accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct
      421 gcgtatgatt caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg
      481 ccacacggca aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc
      541 caggctggcg gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca
      601 aaaggagcca ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacagacgat
      661 aacctgaccg atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg
      721 aaaaggatat tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacggta
      781 cgggataatc ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa
      841 ggcgataaaa taaccggaga gctgaaaatc cgtggtgtta atgcgctgag gattttcaac
      901 gaagcttttg gcctgatttt tcgtcgttcg gaagagtgcc tgcaccttat tcccaccagt
      961 gaaggtcagg gggaaaatgg cgatattggt cccctgcgcc cgttcaccat taatttgcgg
     1021 acgggtgaaa tatccatgtc gcataaagtg tctgttggcg gtggttcgca ggtcaatggt
     1081 gcgctgggta tcggcgttca gaacgccctg ggggggaatt caattgttct tggtgataat
     1141 gacaccggat ttaaacagaa tggagacggt attctggatg tttatgctaa tagtcagcgt
     1201 gtattccgct ttcagaatgg agtggctatt gcttttaaga atattcaggc cggaactgcc
     1261 agaaaattca cgttatccag cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg
     1321 ggtaatccat ccaggtctgt tgttgcagag cttggcgatg attcaggctg gcattttttc
     1381 agccagagga atacagataa cagcatcacg ttcgccgtaa acggacaggt aattccgtta
     1441 aattacggaa acttcgatgc ccgctataaa tatcgaacag agggggtaca ggatgtacgg
     1501 tatggccatg aaatgtatta cagccccggc agtaacaccg tttcgtggag atttcgcgca
     1561 ccttcgggac acgggctgtc agggatagcg atatcggata ccggccgtga ctcagcggat
     1621 aacgtcaacg gcgtgtatta ccgcccgctt cagaaactga ttaatggcac ctggtataac
     1681 gtggcgagtg tttaacaatg ttgcatatta aaaaatatta ctgcgggcaa tccgaaaacc
     1741 gtggaacaat atcagctgac aaaacaatat gatgtcacct ggcttttttc gaaagacgga
     1801 aaaaactggt atgaagagct gaagaatttc gccagcgaca caattaaaat aacttacacc
     1861 ggagacggtc gcgtggtgtg ggtcggtaag gatgtgactg gcattgaacc ccgtaacgcc
     1921 agtgttattg aagttcctga tattaccgcc aaccgccgca ttaccgcgcc gggttactgg
     1981 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
     2041 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
     2101 ctaatcagcg acgaagaccg ggaaaaactg aaagcgtacc gcatttacgc gaaatcgctg
     2161 caggcgatgg at
//
LOCUS       DAJVOY010000304         2145 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVOY010000304 DAJVOY010000000
VERSION     DAJVOY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.308"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..234
                     /locus_tag="OQL90_004119"
                     /pseudo
     CDS             1..234
                     /locus_tag="OQL90_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="OQL90_004120"
     CDS             655..1233
                     /locus_tag="OQL90_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370683.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKDLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="OQL90_004121"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="OQL90_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..2145
                     /locus_tag="OQL90_004122"
                     /pseudo
     CDS             1584..2145
                     /locus_tag="OQL90_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          687 a          393 c          529 g          536 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatgg catattacca tgcaaattat agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctacttgca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa aggacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttatagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc gcagatgaag ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg
     1861 atatctggag cagaccatgg agcgaggaaa gacgcaacga ctggcagaga tggctgtcgc
     1921 tggcggccaa cagtgacgtc cccatgatga agaatgtggc taaaacgata gggaaaaggt
     1981 tgtacggtat cctgaacgcc atgcgccatg gcgtatcaaa cggaaatgcg gaagcactga
     2041 acagcaagat caggctgctg agaataaaag ccaagggcta ccgcaaccgg gagcgcttta
     2101 agctgggagt gatgttccac tatgggaaac tgaatatggc gttct
//
LOCUS       DAJVOY010000305         2095 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVOY010000305 DAJVOY010000000
VERSION     DAJVOY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.309"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..116
                     /locus_tag="OQL90_004123"
     CDS             <1..116
                     /locus_tag="OQL90_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1370684.1"
                     /translation="VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            113..463
                     /gene="tnpB"
                     /locus_tag="OQL90_004124"
     CDS             113..463
                     /gene="tnpB"
                     /locus_tag="OQL90_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1370685.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            494..2086
                     /locus_tag="OQL90_004125"
     CDS             494..2086
                     /locus_tag="OQL90_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1370686.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNSRKVHTDDTPVKVLAPGRKKAK
                     TGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLSPFRGILQADAFNGYDRLF
                     SAEREGGALTEAGCWAHARRKVHDVYISTKSATAEEALKLIGELYAIEHEIRGLPVSE
                     RLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRYILSV
                     LPEWPSNRVDELLPWNVALTNK"
BASE COUNT          527 a          529 c          602 g          437 t
ORIGIN      
        1 cagttagtgc cagctcctgc aaggtggagt tccgtcacgg taacatgacg ctggaaaatc
       61 cttcaccaga gctgctcaca gtgttgatcc gtgaactgac cgggagggga cgatgatctc
      121 actcccgtca ggcacccgca tctggctcgt tgctgggata accgatatgc gtaagtcttt
      181 caacgggctg ggtgaacagg tacagcatgt gctgaatgat aatcccttct ccggtcacct
      241 gttcatcttc cgtggccgac ggggtgacat gattaaaatc ctgtgggctg atgctgatgg
      301 tctgtgcctg ttcaccagac gcctggagga aggccagttt atctggcctg ctgtgcgtga
      361 cggcaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc tggactggcg
      421 tcagccaaaa acatcccgcc ttaatgcact gacaatgttg taaaaatgtc atggccggat
      481 tataaaaacg gccatgaatc aaaaatacct cattcgcatt gcagaactgg aatgccagct
      541 ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg gaggccttcc tgcgctctgc
      601 actggcccgc gccgaagaaa agatcgaaga agatgaacgg gaaatagaac atctgcgggc
      661 tcagatagaa aaactgcgcc ggatgctgtt cggtacccgt tctgaaaaac tgcgtcgtga
      721 agttgaacag gctgaggccc tgctgaaaca acgcgaacag gacagtgatc gttacagtgg
      781 gcgggaagac gatccgcagg ttccccgcca gttgcgacag tctcgtcatc gtcgcccgtt
      841 accggagcat ctgccccgcg aaataaatcg cctggagcca gaagaaagct gttgcccgga
      901 gtgtggcggt gagctggatt atctggggga agtcagcgca gaacaactgg aactggtgag
      961 cagcgctctg aaagtgatcc gcacagaacg ggtaaaaaaa gcctgtacaa aatgtgactg
     1021 catcgttgaa gcaccggcac catcccgtcc gatagagcgt ggtatcgcgg gcccggggtt
     1081 acttgcccgc gtgttaacgg gaaaatactg cgaacacctg ccactgtatc gtcagagtga
     1141 aatttttgcc cgtcagggtg tcgaactgag ccgtgcatta ctctccaact gggttgacgc
     1201 gtgctgccag ttaatgacgc cgctgaatga tgctctgtac cgttatgtaa tgaacagccg
     1261 caaagttcac actgatgaca caccagtaaa agtgctggca ccgggcagga agaaggcgaa
     1321 aacaggatat atctggacgt atgtccggga tgacaggaat gccggttcgc cagagcctcc
     1381 ggcggtctgg ttcgcctact caccggacca tcagggtaaa catccggagc agcaccttag
     1441 tcccttccgg ggtatcctgc aggcagatgc gtttaatggt tacgatcggc tgttcagtgc
     1501 cgaacgagaa ggcggcgcgt tgacggaagc aggatgctgg gctcatgcgc ggcgcaaagt
     1561 ccacgatgta tatatcagta ccaaaagcgc gacagcggaa gaagccctga aactaatcgg
     1621 tgagctgtac gccatcgagc acgaaatacg cgggttgccg gtgtctgaac gcctggcggt
     1681 caggcaaatg cagagtaaac cgctactgac ttccctgtat aagctgatgc aggagaaaga
     1741 acacacgtta tcgaaaaaat gccgtctgag agatgcgttc cggtatatca ggaagcactg
     1801 ggttgcgttg tgcaacttca gtgatgatgg tctggctgag gcggataata atgccgcgga
     1861 aagagcgctt cgtgcagtct gtctcggaaa gaaaaacttt atgttcttcg gcagcgatca
     1921 cggtggagag cgtggtgcgc tactgtacgg gctgatcggc acctgccgac tgaacggtat
     1981 cgatccggaa gcgtatctgc gctatatcct gagcgtactg ccggaatggc cttccaaccg
     2041 tgttgacgaa ctcctgccat ggaacgtagc actcaccaat aaataagcgt caata
//
LOCUS       DAJVOY010000306         2002 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVOY010000306 DAJVOY010000000
VERSION     DAJVOY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.310"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            111..432
                     /locus_tag="OQL90_004126"
                     /pseudo
     CDS             111..432
                     /locus_tag="OQL90_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(466..672)
                     /locus_tag="OQL90_004127"
     CDS             complement(466..672)
                     /locus_tag="OQL90_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370687.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1170..1243)
                     /locus_tag="OQL90_004128"
     tRNA            complement(1170..1243)
                     /locus_tag="OQL90_004128"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1209..1211),aa:Gly,seq:ccc)
     gene            complement(1322..>2002)
                     /locus_tag="OQL90_004129"
     CDS             complement(1322..>2002)
                     /locus_tag="OQL90_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1370688.1"
                     /translation="CSGSKSSDTGTYSGSVYTVKRGDTLYRISRTTGTSVKELARLNG
                     ISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTTKTASVTPSSAVPKSSWPPVGQRCWL
                     WPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAAGAGKVVYVGNQLRGYGNLIMIKHS
                     EDYITAYAHNDTMLVNNGQSVKTGQKIATMGSTDAASVRLHFQIRYRATAIDPLRYLP
                     PQGSKPKC"
BASE COUNT          522 a          466 c          447 g          567 t
ORIGIN      
        1 tcatacacta aatcagtaag ttggcagcat taccaatttt gctaagttaa tgcattaatc
       61 taaaaacgct ttaaatcaat acataaccat tatctcttgg gattatttac atgcgcaaaa
      121 gtttaatgct gttacttgct tcaacattga ttatttctgg atgtgcaaca aaaaaacaac
      181 tcgttccaac tggtggaagc aaatcagacg gcacagttag gatgagttat tcttacggta
      241 tgtttgaaag tcctgttgta gatccacaac aaggcatgaa tgcagccaaa gcgcgttgtg
      301 ccgcttaggg gatattcagg agccgagcct tttggtggat tcacttctca atgttcacaa
      361 ccttcttcat ctggttgtat gcaaacactt gtgactatcg aatatcaatg cactggcgat
      421 attaagaagt aataattagg ggcttccagc cccttctata accattcatg gaacagggac
      481 aaccgaaatc actttaaacc ctgaaacagc cgttactcca ttagatggag aattaaatct
      541 cacagtatct tttcttactg catcatgaat ttcatctttt gttggttcat gctctgaatg
      601 gaataaaacc tcttccattc taatttgatc accgtctcta tgctcataag taatcttgta
      661 gctaacagtc atatcaacct catgcagggg aattgtgaga ttattataga caatagaaac
      721 cataaagccc cgttggtgat acagcaacaa gcgggagcgt agcgacttca tcagattgtc
      781 agaaagatca caccttatca ggataaccgt gtacccgttg ggagcgtggc tgaaacgctg
      841 atagagcttg catatggttt gcaaggacca atatcaccat aaacctgttt acgcagccac
      901 aaggccgcac atcgggctta aatgcgtgtg ttcagtctgt cgatgacatt acaatgccag
      961 gatgggcttt atcttcacgt tgtgctatcg cggtgtgaga ggtgcaactg ccgccaacgt
     1021 taggttatac tgtcatctca ccgtgtaact taacgtgaca gttactaagg gcggatcaca
     1081 atggtagaac tcctaagcaa attaaatagg taactgttta agttgctgac agagaagact
     1141 tttttagtct tctccacctg gggagattct ggagcgggcg aagggaatcg aaccctcgta
     1201 tagagcttgg gaagctctcg ttctaccatt gaactacgcc cgcttcgaga tgcgtaaggc
     1261 attataaacc ttacgctctc cttagcaagt gccgcgctgc tgactgctga ttaattcgcc
     1321 atcagcattt tggcttgctg ccctgcggcg gcaagtaacg tagcggatca attgccgttg
     1381 cacggtaacg aatctggaaa tgcaggcgaa cagatgccgc atccgtgctc cccatagtgg
     1441 cgattttttg cccagtcttc acgctttgcc cattatttac cagcatcgtg tcattatggg
     1501 cgtaagccgt aatgtaatct tcactgtgtt taatcatgat gagattaccg tagccacgca
     1561 gctggttgcc cacatacacc acctttcctg cacccgcggc gtaaataggt gtaccccgtg
     1621 gagctgagat atcaatccct ttattgccgc catctgctgt cgaatacggc atgataactt
     1681 tccctgtcgt tggccataac caacaacgtt gccctactgg cggccaggaa gatttcggta
     1741 ccgctgatga cggtgtaacc gatgcggttt tggtcgttga tttggcggtt gatttacgtg
     1801 tactactgct acttttcgcc ccacccagtt ttagtttctg accaacttca atggtgtaag
     1861 ggggggaaat gccgttcagt cgcgccagtt cttttacgct ggttcccgtg gtgcgcgaaa
     1921 tacgatatag cgtatccccc cgtttcacgg tgtaaacgga gccggaatat gttcctgtat
     1981 cggatgattt gctacccgaa ca
//
LOCUS       DAJVOY010000307         1976 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVOY010000307 DAJVOY010000000
VERSION     DAJVOY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.311"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            547..1536
                     /locus_tag="OQL90_004130"
     CDS             547..1536
                     /locus_tag="OQL90_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCS1370689.1"
                     /translation="MKNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGA
                     RMASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRK
                     PKDGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
BASE COUNT          439 a          527 c          585 g          425 t
ORIGIN      
        1 aaaaaaaatt cggcgtcccg ctcactgttc ccgaactcgt caaacagaaa atcgcggggg
       61 cccctccggg gttccgattt tgtgattgac gacggcacgg aaaaaaacac tggggatggt
      121 ggttggtgtc acgcagtgag gccaaccacc tgcaagacgg tttttgtttt tcacaaaatc
      181 gctggaaaaa cctgtgagtg ttttgcgggg aaaacgcttt tgtggttgtt gtttttgctg
      241 tccgaaaatc agctcagatt ttgagcaaat ctcgcgcgcg ttccttgtat ttatacttaa
      301 gggataactc taggattcaa attggcctcc agcccttgtc ccgcaaggcc tgagggggga
      361 tttgtcggaa aatggttttg gcgttattct gtcgggagtg gttgcgttaa aagtgacccc
      421 cgcaacgacg ggcatcgttc gggggtgtga ccacctgtag aaattcgaat tagtgacacg
      481 acaacacagg tgatatggca atgataacac gtcagatgag ccatcgccca caatcgggtg
      541 gtgcgcatga aaaaccctaa actccagtac ctcactgatt attccccctc tgatgcgccc
      601 tgggacgcac acagaagcgt ttctgacgac gtgggaggga tttacctgct tgcggccgaa
      661 tatgagcgct acggggctcg tatggcctcc tgtggcggtt tactgcgttt cgggtggtct
      721 accctcaagg aaaccggaga aacgcgtctg aggctgcgtg aggcacattt ctgccgtgtc
      781 cggcactgtc ctgtctgcca gtggaggcgc tccctgatgt ggcaagcccg tttctaccag
      841 tccctgccgc gtatcgtggc ggattatccc gatgcccgct ggatgttcct gaccctgacc
      901 gtgcgcaact gtgcgattgg ggaactgggc gagatgctga accggatgaa tgcagctttt
      961 cagcgtatga aggttcgcaa ggagttcctg cctgttcagg gctggatccg cacaacggaa
     1021 gttacccgcg gcagcgacgg cagcgcacac ccgcacttcc ataccttgat gatggctcct
     1081 cctggcatgc taaatgggaa aagttacgtc aggcatgaac gctgggttga gctatggcgg
     1141 gagtgcctgc gggttgatta tgacccgaat gttgatgtcc gggcagtgaa accccgcaag
     1201 ccgaaggatg gcgagtcctt agcctgcgcg accgctgaac tggtgcgcgg cgcagtggcc
     1261 gaaaccctga aatattccac gaagccggct gacatggtgg ccgatcctga atggttcctc
     1321 gaactgaccc gccagacgca caagcgccgc tttgtggcga ccggcggcgc actcaaagac
     1381 gtgttgaaac tcgatcagga aaccgacgcc gacatggtga tcggggatga tatttccgag
     1441 ggcgacgatg acggttcacg aatcgcgttc gagtggaaaa ccgaggtcag aaagtaccgt
     1501 cgctcaccga cgaaagataa aactgaatca ggctagcggg gtgtcggggc gaagccctga
     1561 ccaggtgggg aatgtctgag cgagcgtgcg cggtccgaca ttcccacatc ctgtcccgaa
     1621 aattcccgcc gtttctcggt tttttgagcg tcgcagaccg cacgacaaac gaaacgaagt
     1681 gtagtttgta tgagtgcgca cttcgtgaga ttccccgact gccagtttgt cgatcgcgcg
     1741 gaacagctgc ggcacgaaaa cggcagaatg atttgctgtc cggttgccag aactgtgcgc
     1801 ttacgtggag ggcggcgcgg cgcaaaaact gccgacattg cgccagtgtt ccggtgacac
     1861 aaacccgcag aaatcgggac aggatgtgta atcgtataac cgcgcacgca cagccatccg
     1921 attaccacct ggtcagggct tcgccccgac accccgccaa cgcttcacgg cacgcg
//
LOCUS       DAJVOY010000308         1936 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVOY010000308 DAJVOY010000000
VERSION     DAJVOY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.312"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OQL90_004131"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OQL90_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCS1370690.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1149..1520)
                     /locus_tag="OQL90_004132"
                     /pseudo
     CDS             complement(1149..1520)
                     /locus_tag="OQL90_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1519..1936
                     /locus_tag="OQL90_004133"
                     /pseudo
     CDS             1519..1936
                     /locus_tag="OQL90_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          610 a          347 c          386 g          593 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc
     1381 agtttttctt catgaagcag atgctccagg aagtccatat aactccattc ctggtctact
     1441 gcctgttgtg acagcgcagg cgctgcgctt atgtactgca cccattttgt tggacgatga
     1501 aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc
     1561 gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg ttttggtgtc
     1621 cccgaaccca ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa
     1681 ttcccaaacc taaaggtgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg
     1741 tgattgagca gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg
     1801 gttctgtagc caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc
     1861 tcaagattgg caccaaaaga aacattgcaa tatcagttga tccagaaaaa gcggcatcag
     1921 cattggagct gtcaaa
//
LOCUS       DAJVOY010000309         1911 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVOY010000309 DAJVOY010000000
VERSION     DAJVOY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.313"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1898
                     /gene="yicI"
                     /locus_tag="OQL90_004134"
     CDS             <1..1898
                     /gene="yicI"
                     /locus_tag="OQL90_004134"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1370691.1"
                     /translation="DTNNQRNYMFERLDLGVGETVYGLGERFTALVRNGQTVETWNRD
                     GGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVSFEVGSEKVSKVQFSVESEYLEYFVID
                     GPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEATVNSFIDGMAERNLPLH
                     VFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKICVWINPYIGQKSPVFKE
                     LQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDF
                     GERIPTDVQWFDGSDPQKMHNHYAHIYNELVWNVLKDTVGEEEAVLFARSASVGAQKF
                     PVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHYIGSFENTAPAHVYKCWCAFGLL
                     SSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARANARGTPMMRAM
                     MMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEGRWTHLWHNDELDGRCW
                     HKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAAD
                     GSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQDGSQAESEQGLVVKPQG
                     NALTITL"
BASE COUNT          439 a          461 c          544 g          467 t
ORIGIN      
        1 aggacacgaa taatcaacga aattatatgt ttgagcggct tgatcttggc gttggcgaaa
       61 cagtttatgg tctgggagag cgttttactg ccctggtgcg caatggtcag acggtagaga
      121 cctggaaccg ggacggcggc acaagtactg aacaggcgta taaaaatatc ccgttctata
      181 tgactaaccg tggctatggg gtgctggtca atcaccccca gagtgtctct tttgaagtgg
      241 gatcggagaa agtctccaaa gtgcagttca gcgttgagag cgaatatctc gaatactttg
      301 ttatcgacgg tccgacgccg aaagcggtac ttgatcgtta tacccgtttt actggtcgtc
      361 cggcgctgcc gcccgcatgg tccttcggcc tgtggctaac cacttcattt accaccaact
      421 acgacgaagc gacggtaaac agctttatcg atggtatggc ggaacgcaat ctgccgctgc
      481 atgttttcca ctttgactgt ttctggatga aagccttcca gtggtgcgat tttgagtggg
      541 acccgctgac tttcccggac ccggaaggga tgatccgccg cctgaaagcg aaagggctga
      601 aaatctgcgt ctggattaac ccctatatcg gtcaaaaatc ccccgtcttt aaagagttac
      661 aagagaaagg ctatttactc aaacgcccgg acggttcgct atggcagtgg gataaatggc
      721 agccaggtct ggcgatttat gactttacca atccggatgc ctgcaaatgg tacgccgaca
      781 aactgaaagg tctggtcgcg atgggcgttg attgctttaa gaccgacttt ggcgaacgta
      841 tcccaactga tgttcagtgg tttgacggtt ccgatccgca gaaaatgcat aaccattatg
      901 cgcacatcta caacgaactg gtgtggaacg tgctcaagga caccgttggt gaggaagaag
      961 ctgtcttgtt tgcccgctcg gcctccgtcg gtgcgcagaa attcccggta cactggggtg
     1021 gcgattgtta cgctaactac gaatcaatgg cggaaagcct gcgcggtggt ttgtctattg
     1081 gcctttcagg ttttggcttc tggagccact atatcggcag ctttgaaaat accgctccgg
     1141 cgcacgttta caaatgctgg tgcgcgtttg gtttgctctc cagccatagc cgtttacacg
     1201 gtagcaaatc ttatcgtgtg ccgtgggcct acgatgatga gtcctgtgat gtggtgcgct
     1261 tcttcacgca actgaaatgc cgcatgatgc cgtatctgta tcgtgaagct gcgcgtgcaa
     1321 acgcgcgggg tacgccgatg atgcgggcca tgatgatgga gttcccggac gatccggctt
     1381 gtgattacct tgaccgtcaa tacatgttag gcgacaacgt gatggttgcg ccggtgttca
     1441 ctgaagcggg cgatgtgcag ttctacctgc cggaaggtcg ctggacacac ctgtggcaca
     1501 acgatgagct cgacggtcgt tgctggcata aacagcagca cagcttcctg agtctgccca
     1561 tttatgtgcg tgataacacc ctactggcgc tgggcaacaa cgatcaacgt cccgattacg
     1621 cgtggcacga aggcacggca ttccacctct ttaatctgca agacgggcat gaagccgtct
     1681 gtgaagtgcc cgctgctgac ggatcggtga tctttacttt aaaagcagca cgtactggca
     1741 acacgattac tgtgactggt gcgggcgagg cgaagaactg gacactgtgc ctgcgcaatg
     1801 ttgtgaaagt aaatggtctg caagacggtt cgcaggctga aagtgagcag gggctggtgg
     1861 tgaagcctca agggaatgcg ctgacaatta cgttgtaatc tattgatgga a
//
LOCUS       DAJVOY010000310         1885 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVOY010000310 DAJVOY010000000
VERSION     DAJVOY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.314"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(30..902)
                     /locus_tag="OQL90_004135"
                     /pseudo
     CDS             complement(30..902)
                     /locus_tag="OQL90_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(916..1056)
                     /locus_tag="OQL90_004136"
     CDS             complement(916..1056)
                     /locus_tag="OQL90_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370692.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVSVFNDNESKITDAAVNLFI
                     QI"
     gene            1195..1866
                     /gene="queE"
                     /locus_tag="OQL90_004137"
     CDS             1195..1866
                     /gene="queE"
                     /locus_tag="OQL90_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1370693.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          557 a          385 c          419 g          524 t
ORIGIN      
        1 cctccgggat ggcatttttt atggttgagt taccagcggc cggatgcgcc gccacctcca
       61 gaagaaccgc caccgccgct aaatccgccg ccacttgagc ctccaccgga accgctgttg
      121 tctgaatgat aacttttacc tgatgctctt ttctgaagta cacataaaca ggtaaagacc
      181 ataaatatgg tgaaagtaaa aaagaaggaa aatacagcaa tggaaaaaat tttatcggag
      241 aacaggaata aatctaaaag atagattgct gaaatacaga ctccactttt acatgcgcga
      301 caaaaattac tgccttgatg aaaaaaccag aaagggaaga acatcactgc aaatacccaa
      361 aaaatagcaa agtaataatg atcactggaa gacgctgatt cactttctga aggattagtc
      421 ggatattggt gttgtgaagt gagttggtta ttcaaagcgt ttatagctag ctctaatccc
      481 tgagcaaatt tttgttgttt aaatgcgggt atcatgttgc tacggatgat atctccagcc
      541 agagcatcgg ttaccttttc ttccagccca taacctacct ggatgcggac agtacgatct
      601 gaccaggcaa caatgatcag tatcccatca ttacgtttgg catctcctaa acgccaattg
      661 tcaaaaactc ttgtagcata ttgttcaatt gtctcgtctt tggttgtggg aacaattaat
      721 acagcaattt gcacttgggt attattgaaa atagattcta acttgtgcgt cagttctttc
      781 ttttcttgtg agtttaagct actcgtaaga tccgtaactt gttactgtaa ttgtgggaca
      841 atagttggtt gggctgcaac aaaggaacat acgaaagtga acattagtat gaaatagcgc
      901 attataaatt tctcattaaa tctggataaa taaattgacg gcggcatcgg taattttaga
      961 ctcgttatca ttaaaaactg atacagaaaa aataaatata attttggtga atgttttgac
     1021 atgattaaat ccattttctt tattttcttc tgacataata cgttactccc tttaattatt
     1081 aacgatatgt ttcgcatttt tctttaaatc tgcatgtaac agaatgttaa taaggcatga
     1141 ttgttgcggc aggatctgca ataattaaca attatccact tcacagagaa tgctatgcag
     1201 tacccgatta acgagatgtt ccagaccctg caaggtgagg gttactttac cggcgtcccc
     1261 gccattttta ttcgtttaca gggatgcccg gttggctgtg cctggtgcga caccaaacac
     1321 acctgggaaa agcttgagga tcgggaagtc tcccttttca gcattctggc gaagaccaaa
     1381 gagagtgata agtggggggc ggcgagcagt gaagatttgc tggctgtcat tggtcggcag
     1441 ggatataccg cgcggcatgt ggtgattacg ggtggtgagc cttgcattca tgatttgctg
     1501 ccactgactg acctgctcga aaagaacggt tttagctgcc agatcgaaac cagtggaact
     1561 catgaagtac gctgcacacc gaatacctgg gttaccgtat cgccaaagct gaacatgcgc
     1621 ggcggctatg acgtgttgtc acaggcactg gagcgagcca acgaaatcaa gcatccagtg
     1681 gggcgcgtac gcgatattga agcactcgat gaactattgg caacgctgac cgatgataaa
     1741 ccgcgagtca ttgcactgca gccgattagc caaaaggatg atgccacacg tttgtgcatt
     1801 gaaacctgca ttgcgcgtaa ttggcgtttg tcgatgcaaa cacataaata tctaaatatt
     1861 gcctgattaa acatttataa gcgtt
//
LOCUS       DAJVOY010000311         1807 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVOY010000311 DAJVOY010000000
VERSION     DAJVOY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.315"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..158
                     /locus_tag="OQL90_004138"
     CDS             <1..158
                     /locus_tag="OQL90_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1370694.1"
                     /translation="IAPLQDAVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEW
                     PANPVRE"
     gene            273..443
                     /locus_tag="OQL90_004139"
     CDS             273..443
                     /locus_tag="OQL90_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370695.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(570..1127)
                     /locus_tag="OQL90_004140"
     CDS             complement(570..1127)
                     /locus_tag="OQL90_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1370696.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1124..1399)
                     /locus_tag="OQL90_004141"
     CDS             complement(1124..1399)
                     /locus_tag="OQL90_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCS1370697.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1585..1710
                     /locus_tag="OQL90_004142"
                     /pseudo
     CDS             1585..1710
                     /locus_tag="OQL90_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          505 a          377 c          440 g          485 t
ORIGIN      
        1 atattgcgcc actgcaggat gctgtagatc tggaaatcgc aacggaggaa gaaagatcgt
       61 tgctggaggc atggaataaa tatcgggtat tgttgaatcg tgttgataca tcaactgcac
      121 ctgatattga gtggcctgca aatcctgtca gggagtaatc attgggatta tgccgcagac
      181 acgtcgtatg caggaacgtg ctgcggttag tttgtgagct ttcgatagtg gttgttattt
      241 ttgcccttat ttgttccgga ggccatggtt caatggtccg tctgccccct gtggtgatgt
      301 cagcaaaatc agccactgcg cgaaccacaa tagcccggga agatgctgaa gatcaccagg
      361 taaagctgtc agcgcagaaa ctggaagaac tgctcgcatc aatggttaag gatgaggttg
      421 atcgcaatga tgggatttat tgacgtcagc gagagcagaa ggaagaactg aataacctga
      481 atgatttacg ctcaatcaga gcgatggcga ttagcggcaa tcacgctcga taaaattaac
      541 cccggtgatc atccggggtt ttgcattcat taaaaccgca tcaacctttc caccaattgt
      601 tctttacggg caacgatcca cccatgttgc tccagataaa atttaaaccg ttccagcgtg
      661 cataccatcg catcggcggg gactttttct gtgaactcga cctgaccgtg tttatcgaag
      721 tggatcagta atgcgcatcc attatttggg gcgggggagt tttgtgctgc aggaggacgt
      781 ttgtggagtt cttcttccat tgcgttaaat ttttcaatgt aagcttcttt tagtctggct
      841 gcttttttgc cagtaaatcc cattacgagg aacatgaagc cgtcttttgt gatcaggtag
      901 catcgtgatt cacgttctgc gccgttgcca atttcgacag tctgaacatc ggccgaaaag
      961 ttggctgatg taaattcggg agagcagtcg agtgtgtcaa tcttagctaa aacgtgttta
     1021 tgttgtttat cgaagtagtc ggcaacagct tgagaggtgg taaccaggcg accatctttg
     1081 aaaaagactt ttggtgactc gttagaaaaa gttatagctg tattcatatg tgatgactcc
     1141 aattaattgg atgatcacca cctctgacgc caatcaggga ggtggtgaga cgtacagggt
     1201 tggcgtaacc ggtcatcaca aaacccggcg cggatttctc cgcccctgca cgccccacca
     1261 taatgcgaat gtggcaatgc ttaacgcaaa aaaaaccgca aacgcggcta tgcgttgtga
     1321 tgttatccga gacgccaatc ccgacaaccg attttgcggt tgtgtgagga atatagcccc
     1381 ggagaacaaa aactgtcaac gggcaaaaaa atttccgtta tcaggaatgt ataaaattaa
     1441 agcgatcgag caaaaaatcc tttctgcaaa actaaaaaag atttcaataa aatcaggcag
     1501 ttaaaaacac ggaaaaacgg tataaaaaag cgcctcaaaa tcctgttttt tctttcagat
     1561 acaatgtctt attgcatttt ttaaaacatg tcagtctggt acatggatat cgataccacc
     1621 gccaattata agctgggcgg tgcacagcaa catgatagcg tacgcctcga tccgtgggtg
     1681 tttatgttct cagcaggata tcgtttttaa ttccgcacaa aaacgacccc gtaatatacg
     1741 gggtcaataa ggacatggca taaagggtaa tgctgccaac ttactgattt agtgtatgat
     1801 ggtgatt
//
LOCUS       DAJVOY010000312         1805 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVOY010000312 DAJVOY010000000
VERSION     DAJVOY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.316"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(316..1464)
                     /locus_tag="OQL90_004143"
     CDS             complement(316..1464)
                     /locus_tag="OQL90_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1370698.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAALNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWSIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          568 a          463 c          394 g          380 t
ORIGIN      
        1 ggttggcgta gttattaaca acgttgccaa aaccccagac agtgataaaa gacatcagcg
       61 caatgttgta ccatcgcagc tgtttttcct gaacattccc agccatagac aaatccttcg
      121 ccaaaattat aggggcatct gtaaaacgag ggattatgcg cggcaaataa gaagtgcgga
      181 aatagcgaaa tgcaaaatta taacctgctg cacattttat ttttaaacga ataaaatttg
      241 gaataaagca agtcagatag aagaaacaaa aaagcagagt gtgaaacagt aagttcacac
      301 tctgcttgaa ttcatttaca cgtgagcaac gggtttcggc gtatgaccgg cgttgcgcag
      361 cagcatcagc aaatagataa acgatacgct ggcgatcatg ataaacaata aattatcgga
      421 gaaattctgc attagcatag cggtaaatga cgggccaagc agacttccca cagtatagct
      481 caacagtaag gcctggttca tcgccaccag ttgatgatgt tcaactttct cgcaagccca
      541 tgccatcgcc accggatata gcgtaaagcc agcggcaccg aggatgaata acgctggggc
      601 catcgccgcc tggctaagca tcgcgatact gccgagaatg acgacaaaga cctgaacacg
      661 caacaccagc agtcgaccaa acttatccgc cagacgtcca atcgaccatt gtccaaggat
      721 acccgcactg accagtaccg ccatccagaa accaatgctg gcattgctca cccctttgtg
      781 attgaggtac agcggcatca ggccatatag agatcccaga acgattcctg agataatgca
      841 gccattcacg ccaagccgcg cctgacggag ttttagcatt gacgtaattg acgtcgaatc
      901 atggttttca gcctgctgat tcagcacacg cgtaaacaac agcggtaaga tccctgccaa
      961 cgtcaaacct gtaacccacg gcaatacgga catcagctcg gttgaaactt tgctgaccag
     1021 taactggcct aaaaacgttc ccacgtaata aaccatcata tacgcagcaa gcaaacgccc
     1081 acggttacgt gacgtcccac tgcacatcag cgcgctctca acaaccaccc aaatcatggc
     1141 acagccgacg cccgcgacaa aacgccaagc caaccagctc cagaatccaa tcatcaggcc
     1201 aaggccggca cagccagcgg caaaaatgaa ggaggccaga taatagctgc ggttaaagcc
     1261 aatgcgctta atgacatacc ctgtcagcaa tgtaccgaca aggttgccgg taaaatagga
     1321 tgagctgaca acgcctacct gccatgtgga catgtgttcc tgggcgagcc aaagcggcac
     1381 gagtgtattt aacgccgcaa tcgccagagt caacaaaagc aggccagaca gcaaaagcat
     1441 gacaggccgg gtatacgtgg acatgaatta aaaccgtgag gaagttcaaa tttcatgcgc
     1501 atcatgccac cgccaaaaca ttgtcaatga gtgaaaatac gcgatatccc agcggcggta
     1561 ttatcgattt atattactca ttcataacgc taatgctgaa gagatgatta gtctcatctg
     1621 attgttttta ttgaactaac acaaatttca tacgcttcgt tcagtcgaaa aatttcatgg
     1681 tcacagcaaa ctcatatttc ccgtctatgc tttcagagca atgcatcgcg tctgatcgat
     1741 ttcagacgta ccactgactc tcgaaaggag aagaggatga ccaaaccgta tgttcgtctt
     1801 gataa
//
LOCUS       DAJVOY010000313         1754 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVOY010000313 DAJVOY010000000
VERSION     DAJVOY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.317"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(61..339)
                     /locus_tag="OQL90_004144"
     CDS             complement(61..339)
                     /locus_tag="OQL90_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370699.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            280..465
                     /locus_tag="OQL90_004145"
     CDS             280..465
                     /locus_tag="OQL90_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370700.1"
                     /translation="MPAGRRGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(507..662)
                     /gene="hokD"
                     /locus_tag="OQL90_004146"
     CDS             complement(507..662)
                     /gene="hokD"
                     /locus_tag="OQL90_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1370701.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1314..1730)
                     /locus_tag="OQL90_004147"
     CDS             complement(1314..1730)
                     /locus_tag="OQL90_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370702.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          432 a          445 c          384 g          493 t
ORIGIN      
        1 ggcttaagga ccaccagccc gagttccgga accagaacag gtcgaagtaa tacccgcaca
       61 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
      121 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      181 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      241 ctgataccag tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggcgcggc
      301 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      361 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      421 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      481 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      541 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      601 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      661 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      721 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtagtgttaa ctggggcttt
      781 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      841 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      901 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      961 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
     1021 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1081 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1141 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1201 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1261 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1321 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1381 tcgcgtttta gttgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1441 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1501 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1561 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1621 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1681 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1741 gccctttcag gcgg
//
LOCUS       DAJVOY010000314         1740 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVOY010000314 DAJVOY010000000
VERSION     DAJVOY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.318"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(891..1364)
                     /locus_tag="OQL90_004148"
                     /pseudo
     CDS             complement(891..1364)
                     /locus_tag="OQL90_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1292..1658)
                     /locus_tag="OQL90_004149"
                     /pseudo
     CDS             complement(1292..1658)
                     /locus_tag="OQL90_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          391 a          485 c          417 g          447 t
ORIGIN      
        1 tcaaaattat caaagcgcag aacctcagct tcttcttcgt caaaaaaaat catcttcgtg
       61 aagttttacg acatagtgga ggagtttgct tctttgaggt gcaggttttc gtttgcgtgg
      121 tttagctgaa cgggccatat aactacctga aagacaatga tattgtctgt ttttataacg
      181 gtaatcacag gccatgacaa gccgcagccg tcaggccgcc tactcggggc ggtttagaac
      241 gcttatttag taccagatct tacttataat aaaggggctt ggcagtttgg ataaactatt
      301 gtccttattc cactaataca aactttttgt aaagtatatt aaatatactt catttcatac
      361 tgaaatcccc ggaagtattc aatcaaacaa ttacaccata aaaggaagag ggggccccaa
      421 ttaagttgtc aagcatgttt actgcgcaat cgcttggtgc tccgtccctg taccccgcat
      481 tggtactctg gcgctcatgg ggcttccact tgcgcttctc ccttcacatc ggggtgacag
      541 gtccacgttc cccacaagag cctgaatcga cttcacgccg cttttatgcc ggatgccgcg
      601 caggcagtaa acatatccca caggaacgac tccaccattg ttttgacatc atctatatgc
      661 tttcgacaca tcatcagcgg ttcactttcg ttcgtctctt cgaaccatac ctgatcggct
      721 cacgtccgac cttttcccat ctcgctcacc accacatctc ttaaatgcag cagcagtgga
      781 tggtttggtg cctgcgcctg cacgccgaca ccgaggggcc ttccctcatc tcttgtgccg
      841 catggctgca cagggactca cgtccccagg acgcgccttc gtggcacaca gtcatcggca
      901 tagcaacagt aagcaacggc aggtttccat tgccagtttt ctttgacagc agtacttcgg
      961 cctcgctgga tactgtgatt ccagtaccag cgatcctttc tggcttttcc actcaggtag
     1021 cgtttattca gatactggtc gaactcattc agtatgatgt tcgacagcaa cggcgagatc
     1081 acaccgccct gtggcatact ttcactgacc gcccggaaga gaccaacatc aacacggcgt
     1141 tttccacagc agtctcataa aacgtgcatc actgatcctg cggcagacgg ctttcatcag
     1201 cagccggtga tgtactgtgt cgaagtagct ggacaggtca ccttcaataa cccggcgccc
     1261 cgtgtttccc cgcagtcggt gagctgtaat agtacgtgat gtctcccgcc cacttctggt
     1321 tcgggccact ggcgtaaaaa tcctgcgtca gcagattttc tgatacaggc aggccgtgtg
     1381 cacggtaact gatcgggctg aacttctggg aggcttttgc gctcagttcc tgacggcgca
     1441 gactggctgc cacggttttt acgttaaagt ggtaaccaga gcacacagtt catccgtcag
     1501 gcgtggggca ccgtaacgct ggactgggta aaagccttgc ggacaacgct gtcgcagtgc
     1561 tgacggaact gctgacgtgt gtttatcctt gtccgccgct gacaccacgt ataccagccg
     1621 ctgcgggcca cccggagtac gcggcacatt gccttgatga cggtttacgc ggtcgcctgc
     1681 ggcaacggct ttccggctga cggtagtgtg gaccttttta ccccggagaa caccggcaag
//
LOCUS       DAJVOY010000315         1727 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVOY010000315 DAJVOY010000000
VERSION     DAJVOY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.319"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..462
                     /locus_tag="OQL90_004150"
                     /pseudo
     CDS             1..462
                     /locus_tag="OQL90_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(479..658)
                     /locus_tag="OQL90_004151"
                     /pseudo
     CDS             complement(479..658)
                     /locus_tag="OQL90_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            988..>1727
                     /gene="ipaH7.8"
                     /locus_tag="OQL90_004152"
     CDS             988..>1727
                     /gene="ipaH7.8"
                     /locus_tag="OQL90_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCS1370703.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQR"
BASE COUNT          530 a          382 c          302 g          513 t
ORIGIN      
        1 ataccgtcat acggacatta aaaagctctc gccccggcgg ataacatctg cgccagtcgc
       61 tcacgctgat gttgccctta tctgtgaact tgatgagcag tggcgcttcg ttggcagcaa
      121 ggcgcaacaa cactggccct ggtatgcgta caacactaaa actggcggtg tgttggctta
      181 cacttgcggg ccacggaatg atgagacctg tcgggaactg ttagccctgt tgactccgtt
      241 ctgtatcggc atggtgacca gcgatgactg gggcagctac gccagagaag tgccggagga
      301 gaaacacctg accggcaaaa tcttcacgca acgtatgaac gtaacaacct gaccctccgc
      361 ccccgcatca agcggctggc acgtaaaaca atctgcttct cgcgttccgt ggaaatccac
      421 gaaaaagtca tcggttcctt cattgaaaag cacatattct aatgattgga agtactaccc
      481 ggcaaaactc attcagtgca ttccagtgat tcaggatata gttgaacgcc tttaccattt
      541 cagcatggtt tgacaaagtt ttcctctgga gctttatcca gtcatacaag gacttcattc
      601 gcggcacaca tttctcctgc cttaccgcaa gccgtttttc tgccggcctc cctcatatta
      661 aatgtgtatc gtttttttac agtaatttta atttatcatt ctataatagg aataaatgag
      721 cttggctaaa atatgatgta aaacaaatat acttactcgg attacatttc cttgctcact
      781 ggaatgttga attcagtgtt attattgact gcagtcacaa taaaaaaaag aattccatag
      841 aaaacctcaa caaataaatt acaactaact tctgttatgt gtaaaatgga aactattaaa
      901 acttaatatc gaaaatggta agtgaaattt gtataaatat acaattttaa atatttattc
      961 tcacaaatat aaggttgccc tagcattatg ttctctgtaa ataatacaca ctcatcagtt
     1021 tcttgctccc cctctattaa ctcaaactca accagtaatg aacattatct gagaatcctg
     1081 actgaatggg aaaagaactc ttctcccggg gaagagcgag gcattgcttt taacagactc
     1141 tcccagtgct ttcagaatca agaagcagta ttaaatttat cagacctaaa tttgacgtct
     1201 cttcccgaat taccaaagca tatttctgct ttgattgtag aaaataataa attaacatca
     1261 ttgccaaagc tgcctgcatt tctcaaagaa cttaatgctg ataataacag gctttctgtg
     1321 ataccagaac ttcctgagtc attaacaact ttaagtgttc gttctaatca actggaaaac
     1381 cttcctgttt tgccaaacca tttaacatca ttatttgttg aaaataacag gctatataat
     1441 ttaccggctc ttcccgaaaa attgaaattt ttacatgttt attataacag gctgacaaca
     1501 ttacccgact taccggataa actggaaatt ctctgtgctc agcgcaataa tctggttact
     1561 tttcctcaat tttctgatag aaacaatatc agacaaaagg aatattattt tcattttaat
     1621 cagataacca ctcttccgga gagtttttca caattagatt caagttacag gattaatatt
     1681 tcagggaatc cattgtcgac tcgcgttctg caatccctgc aaagatt
//
LOCUS       DAJVOY010000316         1668 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVOY010000316 DAJVOY010000000
VERSION     DAJVOY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.320"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(50..649)
                     /locus_tag="OQL90_004153"
     CDS             complement(50..649)
                     /locus_tag="OQL90_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1370704.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPIGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSISFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(688..975)
                     /locus_tag="OQL90_004154"
     CDS             complement(688..975)
                     /locus_tag="OQL90_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370705.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1225..1380)
                     /gene="hokD"
                     /locus_tag="OQL90_004155"
     CDS             complement(1225..1380)
                     /gene="hokD"
                     /locus_tag="OQL90_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1370706.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
BASE COUNT          423 a          444 c          372 g          429 t
ORIGIN      
        1 attctgacgg tgcacatctg cccccgcgcc gctttacgta aatccaccat tacgcaaact
       61 ccagtagctg cgcggccaca ttttcgactt gttccggaga ggaaaattta cggaacagga
      121 tccagttcca cagtacattc agcacagatt tataaacctg ctgaaactcg gtttcgtcca
      181 tattcgcaaa cgagatggat ttcgccctgc gcccacggct accgtccgga taaatatgct
      241 cggtgtaaaa tccggcctga atggttaccc actcgcggaa agcctcaaac gactttagca
      301 atgctgtatc tcgggttctg cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc
      361 tcagagctgg cgtatgttcc cggcctactg attcgcacag gtactcaacg aagcctgata
      421 ccagttctcg ttcgcgaggc gtaatcgccc caccgatcgg agtccagtaa tcgaatccca
      481 gttgcaggag tttgaaaaaa cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg
      541 cgtgtatcca ctcacctgtt ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg
      601 ggagaagtaa tccggaagag gtttgtttga tcagttgtat atgcgccatc ggctttctcc
      661 ggtggcacgg tgttacacag caggagttca atcctgctca agattgtaga tgagtttatt
      721 cttcagcaaa agcagaaaac cagccttaaa accaatctct ttcaaaaccc gtaatgatgt
      781 gacaaattcg tcctcacgca aaataaaacc gtccgtcaga agtccattac aaaaataaaa
      841 taacacagca ccgctcttcc tttgttgaga ttgcaaacat ctaatgcggc aatggctgac
      901 aatcgctcca ttctcaacgc gcacagcata gaggccattt tcactaaaaa tttcacgcaa
      961 ttcttcgatt ttcatcttca gaatccttcc agataaatag ctctcccctg ttcggggtcc
     1021 atccctcttc tccctgcgcg ctacttaagt gagtcgattc tatctgcgaa ggtgcgcgaa
     1081 tcaaattcac cggaaataaa caacaaaaaa cccgccgaaa cgctgagaga tcacagcgat
     1141 gggcgggtta agtgcgggtg cgttgaggat gcctgacaca tcagaggtgg gcggggatgg
     1201 gatcagctcc ccgcccggtc actcttactt cctggattcg tagtctacga agacagcgac
     1261 ctccgtctgg ccggttcgga ttcgtacctc acagaggtct ttcctcgtta ccagtgccgt
     1321 cactatgacg gttaaacaga tgacgatcag ggcgattaac atcgcctttt gctgcttcat
     1381 agcctgcttc tccttgcctt tcggcatgta agaggctaac ctacatgtgc aaagcatgaa
     1441 attggcctca gattaatgtt aagcgtcttg ccggacgcgt aatgttaact ggggcttttc
     1501 tctatctgcc ttttggtgtt catgcctgaa gcagatagcc tgaagcagat agcctgaagc
     1561 agatagcctc aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa
     1621 aggtgcccca taaaatcact ccttctcttc tttaccgtag tggagttg
//
LOCUS       DAJVOY010000317         1627 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVOY010000317 DAJVOY010000000
VERSION     DAJVOY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.321"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OQL90_004156"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OQL90_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCS1370707.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNV"
     gene            complement(1183..>1627)
                     /gene="ipaH7.8"
                     /locus_tag="OQL90_004157"
     CDS             complement(1183..>1627)
                     /gene="ipaH7.8"
                     /locus_tag="OQL90_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCS1370708.1"
                     /translation="IEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMV
                     RSREENEFKDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYSQRVADRLKASGL
                     SGDTDAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSRLHHS"
BASE COUNT          425 a          352 c          357 g          493 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagcctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtcg
     1201 tgagccgttt tcagacaatc gcagggccag tacctcgtca gtcaactgac ggtaaatctg
     1261 ctgttcagtc tcacgcatca cctgtgcacc ggcttccctc tccgcatccg tatcaccgct
     1321 cagacctgat gctttcagcc ggtcagccac cctctgagag tactcattct ccagcatctc
     1381 atacttctgc tcttctgcct gcgcccagcg gtcagcttcc gtacgcttca gtacagcatg
     1441 ccatggtccc cagagggaga accagtcctt aaattcattc tcttcacggc ttctgaccat
     1501 ggcttcggca gtgcggaggt catttgctgt cactcccgac acgccataga aacgcatttc
     1561 cttcacggca gtggagagct gaagtttctc tgcgagcatg gtctggaagg ccaggtagac
     1621 ttctatc
//
LOCUS       DAJVOY010000318         1606 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVOY010000318 DAJVOY010000000
VERSION     DAJVOY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.322"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>1606
                     /locus_tag="OQL90_004158"
     CDS             <1..>1606
                     /locus_tag="OQL90_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCS1370709.1"
                     /translation="KGGKDGITMTFIGPSPSVQDKSASTDNTNINGEDMTEIEESMLL
                     PVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTALAISHKTSLLDSL
                     EIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTDRGLLTKEWMKGNRVSRI
                     TRTASGANAGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAKEVV
                     LKKESPFKEWSAALRAPPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYINAYLTETD
                     HENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATTETMEPD
                     TTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEFVEG
                     ISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQQEP
                     EKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVEVQENDPEEMEGAEHPHKENA
                     GSAQDHASDSKTGETADPLITVNGHH"
BASE COUNT          490 a          409 c          395 g          312 t
ORIGIN      
        1 aaaaaggcgg gaaagatggc atcacaatga catttatcgg cccttccccc tctgttcagg
       61 acaaatcagc cagcactgat aataccaaca tcaacggcga agacatgact gagattgagg
      121 agagcatgct tctacctgtc tccggtcagg aactgcccat tcgttggctt gctcaacacg
      181 gcagcgaaaa accagtaacg cacgtttcac gcgacgaact ccaggcatta cacattgcac
      241 gggctgaaga actaccggct gttactgccc tggctatttc gcataaaacc agtctgctcg
      301 actcgctgga gattcgcgac ctccacaaac tggttcgtga cactgacaaa gttttcccta
      361 atcctggtaa ttcagacctg ggactaataa ctgctttttt cgaagcatac ttggacgctg
      421 actacactga tcggggcctg ctgacaaaag agtggatgaa aggaaatcgt gtttcacgca
      481 tcacccgcac ggcttccggt gctaatgctg gtggtgggaa caaaaccgat cgcaatccga
      541 atttagtaca caccctcgac acactggatg tggagattgc agcagccaca cttccgatgg
      601 attttaatat ttatgaaatt ccgggcagcg tttatcgtcg cgcaaaagaa gtagtcctga
      661 aaaaagaaag tccgttcaaa gaatggtccg cagcacttcg tgcacccccg ggtattctgg
      721 actattcccg cgccgctatt tttgcactta tccgaagcgc acaccctgaa ttttatcact
      781 acccgggacg ccttcagggg tatatcaacg cctatttgac ggaaactgat cacgagaacc
      841 ccagcaagga aactctcaca gctacccggc atacgccgga aaaagatatc ctggaagaaa
      901 ttaaccgcga ggtggttact gagcgtgaaa cagaagaaga aaaaccacaa ccatctgacg
      961 caatggcagg tgaacaggca acaactgaaa caatggaacc ggatacaact gaacattgcc
     1021 agaacgcgca gtcgctggat gctcagtcgc aggtgagttc cgctaaccaa gtaaaagtca
     1081 ccgctgacga agtaaacaaa attatgcagg cagccaatat cagccagcct gacgccgata
     1141 agttacttgc tgtatcgcgt ggtgaatttg ttgaggggat tagcgaccct aatgatccga
     1201 aatgggtcaa ggggatccag actcgcgatt ctgtgaacca gaaccagcat gaatcggaac
     1261 ggaacgacca aaaagcggaa caaaacagcc caaatgcgtt acaaaacgag ccagaaacga
     1321 aacaatccga accagtagcg caacaggaac cggaaaaagt ctgcaccgcc tgcggtcaga
     1381 gcggtggcgg caactaccct gattgtggcg cggtgatgga cgacgcaaca taccagaaaa
     1441 cattcgatga agagaatcag gttgaagttc aggaaaatga tccggaggaa atggaaggcg
     1501 ctgaacatcc acacaaggag aatgctggca gcgctcagga ccacgccagc gatagtaaaa
     1561 ctggcgagac ggcagatccc ttaattacgg tgaatggtca tcacga
//
LOCUS       DAJVOY010000319         1604 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVOY010000319 DAJVOY010000000
VERSION     DAJVOY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.323"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1568
                     /locus_tag="OQL90_004159"
     CDS             <1..1568
                     /locus_tag="OQL90_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCS1370710.1"
                     /translation="IRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQ
                     PEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIR
                     AKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDE
                     WENLHALKEKRLNGMPKTTPKSGQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIE
                     RSIIPPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSA
                     IDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFL
                     FNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCST
                     YQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNE
                     EVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALL
                     LGKTGEPPNYLYL"
BASE COUNT          554 a          304 c          311 g          435 t
ORIGIN      
        1 aaatcaggga aaatatgtct gagtttgcac gttgccatta tgggtatata caaatccctc
       61 cagtgacaac tttccgcgcc gacggaccag aaactcccga agaagaaaaa ggttactggt
      121 ttcatgctta tcaacccgaa gatctttgta ccatccataa cccaatggga gatttgcagg
      181 attttatcgc attggttaaa gatgctaaaa aatttggtat cgatatcatt cctgattata
      241 catttaactt tatgggaatc gggggtagtg gtaaaaatga cctggattat ccctctgctg
      301 atatacgagc gaagatcagt aaagatatag aaagcggtat ccctggctat tggcaaggtc
      361 aggttttgat tccattcatt aaagatccag taacaaaaga acgtaaacaa atccatccag
      421 aagatataca tctcactgca aaagacttcg aagcaagtaa agataacatc tctaaggatg
      481 aatgggaaaa cctccatgca ttaaaagaaa agcgtttaaa tggaatgcct aaaacaacac
      541 ccaaaagtgg ccaggttatt atgttgcaaa atcaatacgt tcgagaaatg cgaaaatatg
      601 gcgtacgagg tttacgttat gatgcggcaa aacactcaaa acatgaacaa atagagaggt
      661 cgataatccc accgcttaaa aattataatg agcggttaca caatactaat ttatttaacc
      721 caaaatatca aaaaaaagcc gttatgaatt acatggaata cctggtaact tgtcagttgg
      781 atgaacaaca aatgtcatca ctgctttatg agagagatga tttaagtgcc attgattttt
      841 ctttactcat gaaaacgata aaagcctttt catttggtgg agatctccaa acccttgcat
      901 caaaaccggg ttccacaatc tcaagtatcc catcagaaag acggatattg attaacatta
      961 accacgattt tcctaacaat ggtaatcttt tcaatgactt tctatttaac catcaacaag
     1021 atgaacaatt agcaatggca tatatggccg ctctcccgtt cagcagacct ttagtttact
     1081 gggatggcca agtattaaaa tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg
     1141 tcggcggtga ggcgtggctt aataaaggtt gctctaccta tcagcagctc tacaatgaat
     1201 tccacgcatt atatatagat aaagcaggaa tatggagcgc atttgagggt gtatttgcaa
     1261 ctaaaaacgt tctggccttt agtcgtgggg attctgtgaa cattaatcac tctcctcatg
     1321 atgggctagt tataataaat aaaggaaacg aagaagttga aggtacctgg cctaacaaat
     1381 tgcaacctgg aatatacaaa aacatgggga gtaatagcgt taacattatt attaataata
     1441 cccgaaaaat tatcccccct ggtaaagtat ttacgcttag aggcggaact ctaaatatca
     1501 atattcctgg gcgtagcgct cttcttttag ggaaaacagg agaaccgccg aactatctct
     1561 atttataatt taatgttata tctgccccga taaaacgggg caga
//
LOCUS       DAJVOY010000320         1548 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVOY010000320 DAJVOY010000000
VERSION     DAJVOY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.324"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..629
                     /locus_tag="OQL90_004160"
     CDS             <1..629
                     /locus_tag="OQL90_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1370711.1"
                     /translation="DQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPP
                     VSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRT
                     RIKLFYRPDGLKGNPSEAPLALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKR
                     YPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGTIEGNASL"
     gene            664..1548
                     /locus_tag="OQL90_004161"
                     /pseudo
     CDS             664..1548
                     /locus_tag="OQL90_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          451 a          335 c          340 g          422 t
ORIGIN      
        1 gcgatcaaaa aagtgtcaac gtacgtctgg aaaataaagg gaataacccg ttgcttgtcc
       61 agagttggtt agatactggc gatgacaacg ctgagcctgg cagtattaca gtccctttta
      121 ctgccacgcc gccagtatcg cgtattgatg ccaaacgtgg gcaaacaatc aaattaatgt
      181 acacagccag cacctcactg cctaaagaca gagagagcgt gttctggttt aacgtactgg
      241 aagttccacc aaaaccagat gcagaaaagg tcgcgaatca aagtctgctg caactggcat
      301 ttcgcacacg tataaaactt ttctatcgcc cggatggatt gaagggaaat ccctctgaag
      361 ccccgttagc cctgaagtgg ttctggtcag gttcagaagg taaggcgtca ttgcgagtga
      421 ccaatccaac cccttactac gtctctttta gcagtggtga tttagaagct agcggtaaac
      481 gctatccgat tgatgtgaaa atgattgcac catttagtga tgaggtcatg aaagtcactg
      541 gccttaatgg caaagcaagt tctgcaaaag tacattttta cgccattaat gattttggtg
      601 gcaccattga aggtaatgcc agtctgtaat caggcaagga tataattccg caggaagcat
      661 aacgtgacta taaaaccaac taaaaattat cataatcatc tgacccgtat cgctacgttt
      721 tgcgcgctgc tgtattgcaa tagtgctttc tgtgctgaac ttgttgaata tgaccatacc
      781 ttcctgatgg ggcagaatgc ctcaaatatt gatctcagcc ggtatagtga aggtaaccct
      841 gctataccgg gtatgtacga cgtcagtgtt tatgtcaacg atcaacctat tattaaccaa
      901 agcattacat ttattgaaat tgaaggaaaa aaaacgcaca agcctgtatc acacttaaga
      961 atttattgca gtttcatata aattctcccg atataaataa cgaaaaagcc gttctgcttg
     1021 ccagggatga aacactcggc aattgcctta atttgacgga aattatccct caggcttctg
     1081 ttcgttatga cgttaacgag caacgtctgg atatagacgt tcctcaggca tgggtaatga
     1141 aaaattacca aaactatgtt gatccatcgt tatgggaaaa tggcattaat gcggccatgt
     1201 tgtcatacaa cctcaacgga tatcatagtg aaactcctgg tcgaagaaat gacagcattt
     1261 atgctgcttt taacggtggg atgaatttag gtgcatggcg actgcgtgcc tcaggcaact
     1321 acaactggat gaccgattct ggcagtaatt atgattttaa gaatcggtat atacagcgtg
     1381 atatcgcctc gctgcgttct caactcattc ttggtgagtc ttatacaaca ggcgaaacct
     1441 ttgattctgt cagtatccgc ggcattcgtt tatacagcga cagccgcatg ttgcctccga
     1501 cattagcgag ttttgcgcct atcattcatg gcgttgctaa taccaacg
//
LOCUS       DAJVOY010000321         1546 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVOY010000321 DAJVOY010000000
VERSION     DAJVOY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.325"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(918..1394)
                     /locus_tag="OQL90_004162"
     CDS             complement(918..1394)
                     /locus_tag="OQL90_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1370712.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          352 a          413 c          383 g          398 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc catgattttg cccgatttcg
      181 cgctctccac gttcaacagc tcgatcatgt ttttgccgtc cgttcaccgt taaaacgcct
      241 cagaaatcgg gacaggatgt gggaatgtcg gaccgcgcac gcgcactcag acattcccca
      301 cctggtcagg gcttcacccc gacaccctgc atccgtgcgt aagtgctcaa tgtaacttcc
      361 ggttctcttc cgcttcctcg tcgattcgct cgctacgctc ggtcatcgac ttgcggcgag
      421 cgggatcacc tctcgcatgt tgtcggtgat cccgttgttg ttctttcggg gatgagtcgt
      481 cagatagccc ttcaaacacg ctcagaagct ctcagacgca cgaaacgggt ttaaatgccc
      541 gccagggcat ggaaggcgcg aaaaacgctt acagaggctc tcaggaggtc aggcgcgccg
      601 atacccctaa ccaggggctt tcaggtcgct aggaaccgcg tcagctccct gcaaagccta
      661 tttgcacggt acaggcagct attcggctgg ttggtggtcg tagaccgtcg gtgttacagc
      721 gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg ctgccggaac
      781 cgtggcgacg cggtcgttct tgggatcggg gagcaaaaac acgcaaggga ttttgaggcg
      841 gcaaaagagc aatgcaggat agacaatcat ggtcaaaacg ttaacacgta gcccctgatt
      901 gtccgcctgc cgctggctca atttgaaaac atggccatga ttcggcgctg aatatcaccg
      961 tctttgttga ccagtgtttt agtgacaaac gctgcatggt ttttcgtagt gttgtatccc
     1021 ttccagactt catgtggatc gagggcatac gcacaaacct taccagcaac ggggattcta
     1081 actaatgcat tttttgattc taagtaattg agccaccgaa taattgtgct tctgctaact
     1141 ccaagttttt cagctaggaa ttgctgatcc gcaacgacag cgccacacgt aggatcgata
     1201 tgctccgcta aaaatgagta cagcttcagt gctgatatgc cttggctatc cttcagcagc
     1261 tctcgaacgc gttcccaacc ttttggggat acctgtgtaa accttgaatt cttctttgct
     1321 tcttcgagat cctcggtgtt tttctttgcc tcatctgaaa gttgtttcag tcgtttttct
     1381 gaatcaataa acatgatttt ttccctctga aaatcccatg cactaacgtt ggttcaaatc
     1441 taagccatca tgacacatct gtgcaacacc ttgttgcgtg ttgtcgcatc caggatgcga
     1501 caacgttgtg tcaccctgga tgcgacaggt aaacaaaaaa cacact
//
LOCUS       DAJVOY010000322         1519 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVOY010000322 DAJVOY010000000
VERSION     DAJVOY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.326"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..1519)
                     /locus_tag="OQL90_004163"
                     /pseudo
     CDS             complement(1..1519)
                     /locus_tag="OQL90_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          389 a          386 c          332 g          412 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctactcctgc caatttttgg ccttcatctg atgagtccag
      301 gccagcaaag acgccacctc tttaccatcc catgcgcggg atttagcgta agccgtttgt
      361 gtcgtctggt cagtttggag aatgttgcca tgcaccgtac gccagacagt aaaggtctct
      421 gcctgaccat ttttcaccgt aatggtttcc tcacggctta acattttcac ccacttacaa
      481 ttatgtaagt agtagcctgg tttctctgcc gacagccgtt cagcaaaaat atcgacatca
      541 tcgccgaaac ctgccgttga tccccaggaa atcacaccat tatgaccaaa aaccagccca
      601 ggataggcaa atggtgtatt gccagtgaca tcataaccag caccgtgcag accaatacca
      661 taagtatacg caggcgcata ccagccaaac tgcggaccat ttaccatgat tgctttcgca
      721 tcctgggctt tgcttttgcc gatcacccac atattgctgg tcgttggata ccccgccaga
      781 ccattggcac caccctgtgc aaattgtgca gcaatagttt cccggttctt ccctgctgtt
      841 aacgccagca gtgcgccatc cgcccctttt gctggtcggt caagcattgg tgcaggtaaa
      901 tcgtagcgtg gcaacagagc tgctgtttgc gagttttgct gattaaattt aagtgggtag
      961 ctactctctt gtacggcaat agtggttggc gctgatgggt ttaccagcca tttcaactga
     1021 ttaaataccg ccatgccttg tgatacacca tatttatctt ttaaagccgt tagcagtgcc
     1081 agattatcaa tttcgctagt gctatcagag aagcggtttg ccatggtgcc cacaaatatc
     1141 atcgcgacat caaacggttc ccagcgctta ggagtaaagc caaatgtatt aaactgtttt
     1201 ggtaagagcg tctctggatt ggtatttacc ttatcaatcc aggcattcat tccatcagcg
     1261 tagccttgca gaatggacat atcctctggg gaaagggcat caatttgcgc ccggatagca
     1321 tccggccagt agttacgacg gatatcttta tcaaatttca caaaatcttt gccaagcact
     1381 tccgcgacag tcccttgagt actgcgacgt gccatttcca tctgaaaaag gcgatcttgt
     1441 gctactacat agccatagcc ataaaatagg tgccatgtat cattggcata aatatgcggc
     1501 atgccgtatt catcgcgaa
//
LOCUS       DAJVOY010000323         1513 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVOY010000323 DAJVOY010000000
VERSION     DAJVOY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.327"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1304
                     /locus_tag="OQL90_004164"
     CDS             <1..1304
                     /locus_tag="OQL90_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS1370713.1"
                     /translation="TGLDIAELQSQFAGFGASAKESMGIQGSEELFRNMIGYSRLMGR
                     SEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKSEQELFDAMKK
                     GALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSENG
                     LQDFTRSLTTLLNSLGGSGKSLGESLGSLMTSMSSGVDSLTDISYRVRAFFDEVTLAY
                     RGLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSIYGLMTAITRLGQVADTT
                     ADNVNRKNGKPSRLNPMKMLLAGSAIYAGYERLASTPERMEAQAKLPELAKESGLKPA
                     FTMDDVKGWFNSAAMNLANFQHPELNNIKQDSALTSADIQSLRDEISALSKRIQEPVK
                     VSLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPEDD"
BASE COUNT          488 a          256 c          345 g          424 t
ORIGIN      
        1 caacgggttt agatatcgct gaactgcaat cacagtttgc tggttttggt gcttcagcta
       61 aagagtctat gggtattcaa ggttctgaag agctttttag aaatatgatt ggctattctc
      121 gtttaatggg tcgaagtgag gaagagatta agcgagcact gacagcatta tcacagatgg
      181 ctggtaaggg tcagattatg gctgaagagc ttaaggggca actagctgaa gctgttcctg
      241 gtatggttca ggtattcgct aaagcaaccg gaaagagtga acaggaatta tttgatgcca
      301 tgaagaaggg ggctttaaaa tctgccgata ctcttcagaa ggtcactcag gaactgaata
      361 aacaaatcac agctaaaggt ggctggaaag ctatttcaga aagcacacaa gcacaactgg
      421 ggaacctgaa aaactcttgg aataccacgc ttgatagtat cttcagaggt tctgaaaatg
      481 gtctgcaaga ttttacacga agcctaacaa cgcttttaaa cagtttaggt ggaagtggta
      541 agagtcttgg tgaatctctt ggtagcttga tgacttctat gtcaagtggt gtggatagcc
      601 tgaccgatat cagttacaga gtgagagcat tctttgatga ggtgacatta gcttatcgtg
      661 gtttaaccga tgaacaaaag aaagttgttg atgctttagg ttctggcttg attaatagtt
      721 taaaagtctt ggctggtgtt attgctgtga ataaagttgt tggtgtaacc agaagtattt
      781 acgggcttat gactgctatc acaagattag gtcaggtagc tgatactaca gcggataacg
      841 tgaatagaaa gaatgggaaa ccatcaagac taaaccctat gaagatgcta ttagctggta
      901 gtgctatcta tgcaggttat gaaagattag cttctacacc tgaacgaatg gaagcacaag
      961 ctaaattacc agagctggct aaagagtctg gacttaaacc cgctttcact atggatgatg
     1021 ttaaagggtg gtttaatagt gctgctatga atcttgctaa cttccagcat ccagaattaa
     1081 acaacattaa gcaagattca gcattaacga gtgcagatat ccaaagcctg agagatgaaa
     1141 tctccgcttt gagcaaacgt attcaggagc ctgtgaaagt ttcacttggt ggtgaagttg
     1201 ccattaaacc agatgaaacc agctttatga cctttagcag taatatctac gaccaatatg
     1261 cagaagctac gctattaagt tcatcattcc cagaagatga ttaataatag tcgaatgcta
     1321 cgtgaataaa gcaaagttaa tacccacacc aaaaattatt tttcacgtag ctcacgctta
     1381 acagagaaaa tacttatata tcaaaggttt ggttaaaaat gattaaaata cactaagcct
     1441 ttgaaatcaa aggagaattt aatgatttac tctaatcaac ttcgttacac tggcaaagat
     1501 ggcgtttact tcc
//
LOCUS       DAJVOY010000324         1512 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVOY010000324 DAJVOY010000000
VERSION     DAJVOY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.328"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..1386)
                     /locus_tag="OQL90_004165"
                     /pseudo
     CDS             complement(1..1386)
                     /locus_tag="OQL90_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          517 a          263 c          222 g          510 t
ORIGIN      
        1 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
       61 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
      121 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
      181 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
      241 tatttttatc aatcagccct ttattcatat taaacctgtt aataaaatgg atcatctgtt
      301 gggtatcgaa atccaccttg ctgaatcgat gttgacgcat aaaatcatcg aacttgttat
      361 cttcaccaga atggatataa ctgcgtataa ttggatcttt tatataagga caatggctaa
      421 atgttttctt catattctca ataaagtttt tattaacaat attaagaatt tttaattgca
      481 taaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
      541 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
      601 aatcaatagt taattccaat tgttctgtaa tgcaatcttt aaaattccct gtcgacattc
      661 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
      721 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
      781 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
      841 tatcaagaat atcttggtta aaatcaacac attcgccact cgttttgaga aaaatcacat
      901 tgtgtaaacg tcgacattta aatatgacat tatcaactgt tgccttagta aaattgcaat
      961 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
     1021 tagaaataac gtcaccttta aaatgacaat ttgttacatc aaaatcagac aggtcgatat
     1081 acccctgcga atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
     1141 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
     1201 gattttttcg ttccgttgtt ttatatttaa aaatatatta ctgatataaa taaagttatc
     1261 tctgtttaaa cctgtcaccc cattgaaatt acgtagtaaa tcaagatcta ctatattatt
     1321 tccttgaacc acatcatgtt tcatcaaaga gttattgcca ccagaatttg tgatatgacc
     1381 tatcataaaa cctctgtaat acagaaataa aataacctgg aaaagtagcc tgagcgtcca
     1441 gacactaccg gcgaaaggaa aagaatctct gataaggcat tgagataatg gacattctta
     1501 gtgaagaaga ag
//
LOCUS       DAJVOY010000325         1503 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVOY010000325 DAJVOY010000000
VERSION     DAJVOY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.329"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            107..467
                     /locus_tag="OQL90_004166"
                     /pseudo
     CDS             107..467
                     /locus_tag="OQL90_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            554..1503
                     /gene="istA"
                     /locus_tag="OQL90_004167"
                     /pseudo
     CDS             554..1503
                     /gene="istA"
                     /locus_tag="OQL90_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          372 a          390 c          383 g          358 t
ORIGIN      
        1 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
       61 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
      121 ctttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
      181 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
      241 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gcttcaacgg tgccggagtt
      301 cgcgatactg cccgtacgct gaaaatcggc atcaataccg tcatacggac attaaaaagc
      361 tcccgccccg gcggataaca tctgcgccag tcgctcacgc tgatgttgcc cttatctgtg
      421 aacttgatgg gcagtggagc ttcgttggca gcaaggcgca acaacactgt cacgaacggt
      481 gcaatagtga tccacaccca acgcctgaaa tcagatccag ggggtaatct gctctcctga
      541 ttcaggagag cttatggtca cttttgagac agttatggaa attaaaatcc tgcacaagca
      601 gggaatgagt agccgggcga ttgccagaga actggggctc tcccgcaata ccgttaaacg
      661 ttatttgcag gcaaaatctg agccgccaaa atatacgccg cgacctgctg ttgcttcact
      721 cctggatgaa taccgggatt atattcgtca acgcatcgcc gatgctcatc cttacaaaat
      781 cccggcaacg gtaatcgctc gcgagatcag agaccaggga tatcgtggcg gaatgaccat
      841 tctcagggca ttcattcgtt ctctctcggt tcctcaggag caggagcctg ccgttcggtt
      901 cgaaactgaa cccggacgac agatgcaggt tgactggggc actatgcgta atggtcgctc
      961 accgcttcac gtgttcgttg ctgttcccgg atacagccga atgttgtaca tcgaattcac
     1021 tgacaatatg cgttatgaca cgctggagac ctgccatcgt aatgcgttcc gcttctttgg
     1081 tggtgtgccg cgcgaagtgt tgtatgacaa tatgaaaact gtggttctgc aacgtgacgc
     1141 atatcagacc ggtcagcacc ggttccatcc ttcgctgtgg cagttcggca aggagatggg
     1201 cttctctccc cgactgtgtc gccccttcag ggcacagact aaaggtaagg tggaacggat
     1261 ggaacagttt ttacatccca ctaatgactc gcctgcgccc gatggggagc actgtcgatg
     1321 ttgaaacagc caaccaccac ggtctgcgct ggctgcacga tgtcgctaac caacgaaagc
     1381 atgaaacaat ccaggcccgt ccctgtgatc gctggctcga agagcagcag tccatgctgg
     1441 cactgcctcc ggagaaaaaa gagtatgacg tgcatcctgg tgaaaatctg gtgagcttcg
     1501 aca
//
LOCUS       DAJVOY010000326         1491 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVOY010000326 DAJVOY010000000
VERSION     DAJVOY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.330"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            11..85
                     /gene="yoaJ"
                     /locus_tag="OQL90_004168"
     CDS             11..85
                     /gene="yoaJ"
                     /locus_tag="OQL90_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCS1370714.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(144..392)
                     /locus_tag="OQL90_004169"
     CDS             complement(144..392)
                     /locus_tag="OQL90_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1370715.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(540..722)
                     /locus_tag="OQL90_004170"
     CDS             complement(540..722)
                     /locus_tag="OQL90_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCS1370716.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(726..1086)
                     /gene="yeaR"
                     /locus_tag="OQL90_004171"
                     /pseudo
     CDS             complement(726..1086)
                     /gene="yeaR"
                     /locus_tag="OQL90_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
     gene            complement(1259..>1491)
                     /locus_tag="OQL90_004172"
     CDS             complement(1259..>1491)
                     /locus_tag="OQL90_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCS1370717.1"
                     /translation="FFVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVTQ
                     YIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
BASE COUNT          420 a          360 c          330 g          381 t
ORIGIN      
        1 gggcataaag atgaaaaaaa caacgattat tatgatgggt gtggcgatta ttgtcgtact
       61 cggcactgag ctgggatggt ggtaacgtca cctctaaaaa atagcaaagg ctgcctgtgt
      121 gcagcctttg tgcaatttaa gcgttaactt ttaatcttcc tgtagataaa tagcacgaca
      181 atcgcaccaa taacggcaac cacgaagctg ccaaaattga agccatcgac tttaccaaag
      241 ccaaacagcg tgctgatcca tccgccgact acggcaccga ctatccccag caggatagtc
      301 ataaagaatc caccaccatc tttacctggc atgatccact tcgccagaat accggcaata
      361 agcccaaaaa taatccatga cagaatgccc attgtttcct cacttatctg ttttgcatta
      421 gcgggttagt cgctgataaa aagcatagca caacatcggg agggcaagat ttgtgacgag
      481 catcacggag gatttttttg cgatggcgca gaaattgcgc catcaacgat cagtgataat
      541 taccaaccac aaacatcatg ttcgttttcc gtgtcataag aacgcacggt attcaccaga
      601 tcttttatca cttcagccgc cacttctggc accagcaaag tcatcggcgt ctctgtttca
      661 taatcgacag aaacgccatt actgttattg gtgacggtca cggtatacgt tgctttgccc
      721 atgattcatt tcccgttatg aatgactttc cgttgttgcg caccttccat caggacttca
      781 ggagccacga agaagtcaat gttgacataa gtatcgtcag tcatggcttc aatgttgtgc
      841 cacttttctg gagggaacac cgcaaactgc cccgcttcga taaggatcac ctgatcaggc
      901 tctgcactgt gttcatcagc gtagccgaga tatttgaccg ccccatgcat aacggaaagg
      961 cgtgggtaac ccccctggcg cgttccttta tcaagatgac gttcgaatta ttccggcagg
     1021 cgcagtttgt ttattccaga aaggcgttga gcgcgtatga atataattct gtgggatttg
     1081 cagcatcctt ttccctcctt cggtgaatgc gctgaaaacg gtttattcca gccgtttcag
     1141 ggtacgcctg ataatttgca ttttaaatac catttattgg ttacttttta gcaccatatc
     1201 agagaaaaat cagggaggat tacaagtggg aagcccgcga caacgcgggc tgaatgtatc
     1261 aggattgcag cgtcgccagt cgggcggcga aacccacgaa catcaaacca atcagtgagt
     1321 tgccaacttt agccagtttc tttttggtac gtatgtactg cgtgacaaaa gcaccagaaa
     1381 taatcaggaa gctcaaatag cagaaactca ccagttccag cgtcgtcgcc agaataaaga
     1441 atgaaattcc cgtatgtggg gcattaacat cgataaactg tacgaaaaac g
//
LOCUS       DAJVOY010000327         1488 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVOY010000327 DAJVOY010000000
VERSION     DAJVOY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.331"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..496
                     /gene="argO"
                     /locus_tag="OQL90_004173"
     CDS             <1..496
                     /gene="argO"
                     /locus_tag="OQL90_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1370718.1"
                     /translation="LVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTA
                     MSSNIELASAEVLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFA
                     LGTISASFLWFFGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQ
                     ALFS"
     gene            589..1329
                     /locus_tag="OQL90_004174"
     CDS             589..1329
                     /locus_tag="OQL90_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1370719.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT          353 a          347 c          415 g          373 t
ORIGIN      
        1 tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg
       61 gttgctggcg ctggtcacct ggggcggcgt agtcttcttg ctctggtatg gttttggcgc
      121 ttttaaaaca gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg
      181 cagatggaaa attatcgcca ccatgttggc agtgacctgg ctgaatccgc atgtttacct
      241 ggatactttt gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg
      301 gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg ctattctcgc
      361 agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt
      421 gggatgtgtc atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca
      481 agccttgttc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct
      541 ccagaggtcc tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa
      601 gttatcgccc tagcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg
      661 ccggatggac cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt
      721 gccactcttg cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag
      781 gcagatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa
      841 gatatcagct cagcgaattt acgcacccag ccagattatg attatcagga tggtaaaagt
      901 atcctgaaag gctaccgcgc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa
      961 ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttcagtgtcg
     1021 ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac
     1081 gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat
     1141 agcgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc
     1201 gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat
     1261 caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca
     1321 gcacaataat acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc
     1381 tcctttttct gtaattttct gtaaaaccat gacgtaatca cctatctttg ctcgcggttt
     1441 catcaaggat aggctgactg aaacggttaa gcggacaagg atgcgggg
//
LOCUS       DAJVOY010000328         1485 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVOY010000328 DAJVOY010000000
VERSION     DAJVOY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.332"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..1483
                     /gene="pdeB"
                     /locus_tag="OQL90_004175"
     CDS             <1..1483
                     /gene="pdeB"
                     /locus_tag="OQL90_004175"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1370720.1"
                     /translation="LSIWLAHQQVETSFIEELDTYSSRVAIRANKVATQGKDALQELE
                     RWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPGKISK
                     DGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGNAHNV
                     VITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTKMLQKGWHR
                     QVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLANGKI
                     VGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQHPQQH
                     ISINLESTVLTSEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISRYREA
                     GHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVDALEYKNVTPHIIEMAKTLKLKMV
                     AEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQHL"
BASE COUNT          397 a          347 c          362 g          379 t
ORIGIN      
        1 cttaagcatc tggctggccc atcagcaggt agaaacatcg tttattgaag agctggatac
       61 ctattcctcc cgcgtcgcta ttcgggccaa taaggtggcg acacaaggga aagatgcgct
      121 gcaagagctg gaaagatggc aaggcgctgc ctgtagcgaa gcccatctca tggaaatgcg
      181 tcgggtatct tacagttatc gctatattca ggaagtggtt tatatcgata acaacgttcc
      241 ccagtgttcg tctctggagc atgaaagtcc gcccgatacc ttccccgagc caggtaaaat
      301 ttcgaaagat ggttatcgtg tctggttaac atcgcataac gatttaggca ttatccgtta
      361 catggtcgcc atgggaacgg cacattatgt cgtcatgatc gaccccgctt cctttattga
      421 tgtcattccc tatagctcat ggcaaattga tgccgccatt attggcaatg cccataacgt
      481 tgtcataacc agcagcgatg aaattgctca gggaattatt accaggctac aaaaaacacc
      541 cggtgagcat atcgaaaata atggaatcat ttacgatatc ctgcccttcc cggagatgaa
      601 tatttcgatc atcacctggg cttcaacgaa aatgttgcag aaaggctggc atcggcaagt
      661 ctttatttgg ttaccgctcg ggttggtgat tggcctgctg gcagcgatgt ttgtgctgcg
      721 tattttgcgc cgtatccagt caccgcatca tcggctgcag gatgctatcg aaaatcgtga
      781 tatttgcgtg cactatcagc cgattgtctc cttagccaat ggcaaaattg tcggtgctga
      841 ggcactggcg cgctggccgc aaacagacgg tagttggttg tcaccagata gttttattcc
      901 gctggcacag caaacaggcc tttctgagcc attgacgcta ctgattataa gaagcgtctt
      961 tgaagatatg ggcgactggc tgcgtcagca tccacagcag catatttcga tcaatcttga
     1021 atccaccgtg ctcacctcgg aaaaaatccc gcaattgctg cgtgaaatga tcaatcacta
     1081 tcaggttaat cccagacaga tcgcgcttga actcactgaa cgcgagtttg ccgatccgaa
     1141 aaccagcgcc ccgataattt ctcgctaccg ggaggcgggc catgaaattt atcttgatga
     1201 ttttggtacg ggatattcaa gtttaagtta tttacaggat ctggatgtcg acattctgaa
     1261 gatcgataaa tctttcgttg atgcgctgga atataaaaat gtcacgccgc atatcatcga
     1321 aatggcaaaa acactgaaac tgaaaatggt agcggaggga atcgaaacca gtaaacaaga
     1381 agagtggcta cgccagcatg gcgtgcacta cggtcagggc tggctctaca gcaaggcatt
     1441 accgaaagaa gatttcttac gctgggccga gcaacatttg tgaat
//
LOCUS       DAJVOY010000329         1485 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVOY010000329 DAJVOY010000000
VERSION     DAJVOY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.333"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="OQL90_004176"
     CDS             complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="OQL90_004176"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS1370721.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFSSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHL"
     gene            1160..1441
                     /locus_tag="OQL90_004177"
     CDS             1160..1441
                     /locus_tag="OQL90_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCS1370722.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          429 a          325 c          333 g          398 t
ORIGIN      
        1 taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca atggctgcaa
       61 ttgggcgttt tgtcgaataa actgtcgcat ataaatagcc atttctttgt acggttaaat
      121 ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt aacgagaaaa
      181 aggttttcgt caccgtatca tacacacccc attgcaatgt cggatcatca gacatcactt
      241 cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata taggtcattt
      301 tatagccaga ggaaaaaggc gtatcgcggt aataatagat agaaacgtta ggttcacgct
      361 tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat gagcataaaa
      421 aatggttatc acgggcatag atcaattcat taatataaag atagccacga ataatattca
      481 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
      541 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
      601 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
      661 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
      721 gatgactcat tgctatcaat ttcctgcctg tttatacccg gccattgtaa ggtgaaaacc
      781 ctgggtaccg acgtctttag ataaatctta cggtataaat agcacttttg cctgatttga
      841 aattgatacc tttctaatcg tcacctggat gaaaataatt cattgatatt caaaattaat
      901 ttcctacagg aatgcagagg cggcgggaag atatgccctt aatgccactg aaaaaccaca
      961 taatctgtac agcaataaaa gtcacggcct aatatggcta acaaacaatc aattgtgata
     1021 tatttcacaa aattaatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc
     1081 gatagccaga gtgtaccacg cctcccgtga acaacgccgc gctgtccagg gcatcggctc
     1141 ttttcagagg aattgtttga tggctaccct caccactggc gtggttcttc ttcgctggca
     1201 acttcttagt gccgtaatga tgtttctggc cagcacactc aacatccgtt ttcgtcggtc
     1261 tgattatgtc gggcttgcgg tgatcagcag cggtctgggc gttgtttccg cctgttggtt
     1321 cgcaatgggg ttgcttggca tcacaatggc ggacatcacc gctatctggc acaacatcga
     1381 gtcggtgatg atagaagaga tgaatcagac accgccacaa tggccaatga ttttgacctg
     1441 atacgtagaa gcctccataa cggagtaatg ccgatcagtt aagga
//
LOCUS       DAJVOY010000330         1476 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVOY010000330 DAJVOY010000000
VERSION     DAJVOY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.334"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            3..1258
                     /locus_tag="OQL90_004178"
                     /pseudo
     CDS             3..1258
                     /locus_tag="OQL90_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
BASE COUNT          488 a          216 c          299 g          473 t
ORIGIN      
        1 atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg aaacaggcct
       61 ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg ctttttgaaa
      121 acacactggt gtgttttatg cggttataca atgaactcag acgtaaagta aatgaagagg
      181 atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg cagaatgata
      241 atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt tattcagggt
      301 ttcaggaggt attacatata ctatacaata tgttcaaaat gttgatattg ttagagtgtc
      361 cttgccgggc agaggttcag agtctatcac agattttaaa ggttattatt ggtatggctt
      421 tatggaatat attgaaaaca ttactgcgtg tgatgatgtt ttttctgagt attgtttaga
      481 tgatgaaaat atgagtatcc agccagaaca gataaatacg ccgggaatat ctgatttaga
      541 tactggtatt gatttgtctg gcatatcttt tattcagagt gaaattaata aaacatatgg
      601 attaaaatac gctccagtag atggcgatgg atattgtctg ttaagagcca tactggtttt
      661 aaaagaacat gagtattcat gggcgctggg cggccataag acgcaaaagc aagtatacga
      721 agagtttatt aaaattgttg ataaacaaac gatcgaggct cttgttgata cggcattcta
      781 taatctcagg gaagatgtaa agacgttatt tggcgttaat ctacaatctg acaacaaaat
      841 tcaggggcag ggtggttttt tatcatggag ttttttgtct tttaaaaaag aatttattga
      901 tagttgcttg aatgacaaaa aatgcatact gcatttaccc gaatttatat ttaatgataa
      961 caaggtacgc cttgttttag acaccgaccc agagcagagg gttaatgagg tgaaaaattt
     1021 cctcactgct ctttcagata gcatttgctc attatttatt gttaatagta atatggcatc
     1081 aatctccttg gggaatgaat ccttttcaac agatgacgac cttgagtatg gttatttaat
     1141 aaacactggc aatcattatg atgtttacct ccctcctgaa ctttttgctc aggcttataa
     1201 gttaaaaaat aaggaaatga atgcgcaact cgactattta aatcgttatg caatttaatg
     1261 gcaaaggcat atgctaaaaa ccattgttat tagtctcaca cttttttatt ggtaaatatt
     1321 gtctctgtat tggtaacgcc gaagatattc tgtttagcca caggtgcaat tatcagcggc
     1381 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
     1441 tgtatataga aaaacaggcg ttcaacaagc catttt
//
LOCUS       DAJVOY010000331         1419 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVOY010000331 DAJVOY010000000
VERSION     DAJVOY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.335"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..578
                     /locus_tag="OQL90_004179"
     CDS             <1..578
                     /locus_tag="OQL90_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1370723.1"
                     /translation="VDHQAGLLSLVRDIEPDKFKNNVLALGDQAKYFNLPTILTTSFE
                     TGPNGPLVPELKAQFPDAPYIAHPGNINAWDNEDFVKAVKATGKKQLIIAGVVTEVCV
                     AFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGAQLMTWFGVACELHRDWH
                     NDIEGLATLFSNHIPDYRNLMTCYDTLTKQK"
     gene            complement(613..>1419)
                     /gene="dmsC"
                     /locus_tag="OQL90_004180"
     CDS             complement(613..>1419)
                     /gene="dmsC"
                     /locus_tag="OQL90_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCS1370724.1"
                     /translation="VAGGFIVLALALLKGDLRAEAQQRVIACMFGLWVLMGIGFIASM
                     LHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGWLLAMLKKLSPALRTLWLIV
                     TMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMFMGGPLLGYLLLSLAGVDGW
                     AMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAALVPDYGALMSWRIVLLAVA
                     LCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
BASE COUNT          397 a          389 c          341 g          292 t
ORIGIN      
        1 ttgttgatca ccaggctggt ttactttccc ttgtacggga tatcgaaccc gataagttta
       61 aaaataacgt gctggcgctg ggtgaccagg ctaagtattt caacttacca actattctta
      121 ccaccagttt tgaaaccggt cctaacggcc cattagttcc agaactgaaa gcacaattcc
      181 ccgatgcacc ttacattgct cacccgggaa atattaacgc ctgggataac gaagattttg
      241 taaaagctgt caaagcgaca ggtaaaaaac agttaattat tgccggtgtg gtaaccgaag
      301 tttgcgtggc attcccggcg ctttcagcca ttgaagaagg gtttgacgtc tttgtcgtga
      361 ctgacgcgtc agggaccttt aatgaaatta cccggcattc ggcatgggat cgcatgtcgc
      421 aagctggtgc gcaattgatg acatggttcg gcgtggcgtg cgagctgcat cgcgactggc
      481 ataatgatat tgaaggactg gcgacgttgt tctctaacca cattcctgat tatcgtaatt
      541 tgatgacctg ttatgacacg ttaacgaagc agaaataaaa gagaaccggg gccgaaaccc
      601 cggttgttat gattagcttg cgacggccat ccccacggtc atatgcaaac catagaatac
      661 gccgcgaccg attaattccc ctgccagaag cagaatgaat gatacagaaa gtagcggaac
      721 cgcaggctga taacctttta gctgtggtgc aatccacaag cacagggcaa cggccaaaag
      781 cacgatccgc caggacatca gcgcaccata gtccggcacc agcgctgcgg cctgctgcac
      841 agaactatga atagtcgcca gctctgcgcc ctgcatcact gacaccacgc cactcactac
      901 cagtgccagt acagaaattg ctggcagcag acgcatcgcc cagccatcga cgccagccag
      961 actcaacaac aggtaaccca gtaacgggcc gcccataaac atcgtcagga agaagcccat
     1021 cggcgtccag atgctgtacc aggtcggaac ggtatcaatg ctgttataca cacgcaccat
     1081 catccagaca aagatgacgc caagaaccat cgtcactatc agccacagcg tacgcaatgc
     1141 cggtgacagc tttttcagca ttgccagcaa ccagccgatg ccgcctacgg caaaaaagat
     1201 cgaaccgctg gcgatttcgt tactgagtgc tgaagcccct acccggttga gcgagttaaa
     1261 agcgcgcatt ggtgaaccaa gatggagcat agaggcgata aagccaatgc ccatcagcac
     1321 ccataaacca aacatgcagg cgataacacg ctgctgggct tctgctcgca ggtcgccttt
     1381 gagcagcgcc aaagccagaa cgataaaacc acctgctac
//
LOCUS       DAJVOY010000332         1412 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVOY010000332 DAJVOY010000000
VERSION     DAJVOY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.336"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(339..605)
                     /locus_tag="OQL90_004181"
     CDS             complement(339..605)
                     /locus_tag="OQL90_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370725.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(787..1185)
                     /locus_tag="OQL90_004182"
                     /pseudo
     CDS             complement(787..1185)
                     /locus_tag="OQL90_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          362 a          287 c          295 g          468 t
ORIGIN      
        1 aagccgtcta tgggaaggag tctgctgtta ttccagagaa tggtaattca gtcatatatt
       61 tctccatcta tgacttgtca ataccatcct gatatttgtg ggtcagcaat atattctact
      121 aaaaggtatt taaggtcatt gcgtccctgc actcgaataa tggtgaagaa agcatgatga
      181 atatatcacg ataacgttca gtatgtatcg aggatagatg gagttactcc tctctgaaat
      241 agaattactg ctctactttt tctgtttcca gaaattatta gtaacaaacc ctgatatatt
      301 cttttgaaaa tggagcttaa acgagtctga aattaacttc agatattaga atgccgttgg
      361 ctttgttttt caacaattcc tacttcattt atctttgtca accattgcat ttctgtagca
      421 gtgaatttca acgttttcgt ccggttggta gcaggtattt gtaattcgta tatagctgct
      481 tttttattga aaacagtata tccatctgta gtaacagtag aagataataa tacaggattt
      541 gacacttcaa ggatgatatt ttcttgtttt tgtggaaggc aagggaatat agtttgtgta
      601 agcatattgc ttccttatgt tatattttag taaagaattt attttttaca ttatagtcgg
      661 ctaaatgaag tttactgtaa aaaaacgata cttttttcgt aagcgatgag atgtggagcc
      721 agaaaaatgg ctgcgttacg tcattgaact gtccttgacc cacgatattc agacagtcgt
      781 tgtatcttaa gttgacatta cccgctgacg tcgatattcc cttggtgaga gataacccaa
      841 cgcactatgc ggatggtttt cattgtaatg ctcgatcgcc actgcaaggt tatgcaatgc
      901 cgttcttggt ttcggcatga acgcgatgca gtcttccttc atcgttttca cgaaccgttc
      961 tgctattcca ttactctgag ggctactgac tgccgtcgtg cagggctcca gattcaactc
     1021 tctggcgaac tgccgtgttt catgagccgt ataagccgaa ccgttatcgg ccactggatt
     1081 ggccgttccg gtcctttatc gccatatcgc tgacatgaca tcctgcaccg ttgctttgtc
     1141 ataatctccc gtgcctgctg cccagtctat ggcttcacga tcgcaatatg gaacgtaacg
     1201 ctgtcatcgg gagaatcgtc aatgcggcat tcggttaacg ctgatatgac tttccgtgtc
     1261 aaaccggaat ctctaatttt gacgattttc tttttaggtt taattccctg agggcgacgt
     1321 agcagtaaat ccgacggtga atcacgctga tattcgcggg ttggataccg cattacagag
     1381 gtccgtcata tgagcctgtc actttcttac gt
//
LOCUS       DAJVOY010000333         1405 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVOY010000333 DAJVOY010000000
VERSION     DAJVOY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.337"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..483)
                     /locus_tag="OQL90_004183"
     CDS             complement(1..483)
                     /locus_tag="OQL90_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1370726.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="OQL90_004184"
     CDS             complement(474..776)
                     /locus_tag="OQL90_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1370727.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1048..1320
                     /locus_tag="OQL90_004185"
     CDS             1048..1320
                     /locus_tag="OQL90_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370728.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          405 a          359 c          304 g          337 t
ORIGIN      
        1 tcagacctta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctggata caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcctgaa ccacggcagc ctggcggctg caaatcgggg tgtggcagaa tatgagcttt
     1021 cagagtaacc atccagaatg agttcccatg tcacacaacc tggagcacca gaaagtccac
     1081 accagaatgg tgaaagaggt tctgaaagcc gtcgccagag ccaataatca cccttataag
     1141 tccgtatttg cagactttat caccggtcat ccctcctgca cggtctgttt ctgggaaacc
     1201 ttccacaaaa tgtacccgga ttcaccgtac gaatacgtca cgttctgcca tacctgccgt
     1261 cgttttgatt tatacgaaac agaagcggag atgaaggcgg atgacccgaa gtggtggtga
     1321 ttttcagccc cgaccgctga aacgcctgtt cacagccaac cagtgctgga cgtccttcct
     1381 ggatgcgggc ggtctgcgcg atatc
//
LOCUS       DAJVOY010000334         1387 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVOY010000334 DAJVOY010000000
VERSION     DAJVOY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.338"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            7..690
                     /locus_tag="OQL90_004186"
     CDS             7..690
                     /locus_tag="OQL90_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCS1370729.1"
                     /translation="MKTQHDHYDRLSVRLSVIISRLLAGESLELKALADEFGVSERTL
                     QRDFQQRLIHLDLEHSSGSYRLTCRTGREHSPNTFAFIRNSGISGIIPAQNRRLIRLI
                     TSNSGSSPCLIGHGGLPSPVTQTACFLELAEAIRDYRAVTLLVNGKSYEAVAPRRLIF
                     QSKNWFLVATRTRNLQVFRMEDVNAVTVLDTSFRRKPELDALTASEEFISALPHFRFI
                     SDVIQTFRE"
     gene            729..>1387
                     /locus_tag="OQL90_004187"
     CDS             729..>1387
                     /locus_tag="OQL90_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCS1370730.1"
                     /translation="MNDPVKGEGRMITDASGVTRREGGILGMWSNIFPERNGSNEQKG
                     DSKPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKIIDIPSVGENARRDKEYEYLYRNLLPSLDL
                     ILWVIKGDDRAFSADEHFYNNVLLPAGGGERVLFVLNQVDKIEPFREWDTHLHQPSPA
                     QR"
BASE COUNT          360 a          328 c          346 g          353 t
ORIGIN      
        1 cccactatga aaacacagca tgatcattat gaccgcctgt ccgtcaggct atcggtcatt
       61 atcagccgct tactggctgg cgaatctctg gagcttaaag cgttagctga tgaatttggt
      121 gtctcagaac gcaccctaca gagggatttt cagcaacgcc tgatccatct ggatctcgag
      181 cattccagcg gaagctaccg acttacctgc cgaacgggcc gggagcactc acccaacaca
      241 tttgcgttca tccgtaattc cgggatttca gggattattc cggctcagaa tcgcaggtta
      301 attcgactga ttaccagtaa ctccggaagc tcaccgtgcc ttatagggca tggagggctg
      361 ccatctccag ttacacaaac cgcctgtttt ctggaactgg cggaagctat ccgtgactac
      421 cgcgccgtta cattattagt taacggtaaa agctacgaag cagtagctcc ccgccgtctg
      481 atatttcagt ccaaaaactg gtttcttgtt gctacccgca ccagaaattt acaggtgttt
      541 cggatggaag atgttaacgc cgtaaccgtt ctggatacct cttttcgcag aaagcctgaa
      601 cttgatgctc tgactgccag tgaagagttt atttcagccc tccctcactt tcgtttcata
      661 agtgacgtaa ttcagacctt cagagagtaa cagacaacgt tcatttcaaa ttaaaaagga
      721 gacctcgtat gaatgacccg gtaaagggcg aaggccggat gattactgat gccagtggcg
      781 tcaccagaag ggaaggcggg attttgggaa tgtggagcaa tatatttcca gaacgtaatg
      841 gtagcaacga gcaaaaaggt gatagcaagc cgcacatacc cgacatcagg gatattcttg
      901 atattttccc tcagccactg cgggaaaagc tgttgaacaa aatcagtgaa gccatcgact
      961 atgaacccgt gattggtgtc atgggaaaaa ctggcgcggg gaaaagcagc gtatgtaatg
     1021 cgctgtttaa aggggaggtt tgtgcagtta gcgacgttga ggcatgtact cgcgaagttc
     1081 aggaattgcg tatacgcttc ggcaaacatt cgctaaagat cattgatata cctagtgtcg
     1141 gtgagaatgc ccggcgggac aaggagtatg aatatttata ccgtaatttg cttccctccc
     1201 tggatcttat cctgtgggta ataaaaggag acgatcgggc cttttctgcg gatgagcatt
     1261 tctacaataa tgttctgctc cctgctggtg ggggtgagcg cgttttattc gtcctcaacc
     1321 aggttgataa aattgagcca ttccgcgagt gggatactca tctgcatcag ccatctcctg
     1381 cacagcg
//
LOCUS       DAJVOY010000335         1377 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVOY010000335 DAJVOY010000000
VERSION     DAJVOY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.339"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>1377
                     /gene="rhsC"
                     /locus_tag="OQL90_004188"
     CDS             <1..>1377
                     /gene="rhsC"
                     /locus_tag="OQL90_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HCS1370731.1"
                     /translation="DGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTE
                     YGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDD
                     KYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREV
                     LHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRI
                     TTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRYDNPH
                     SDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQY
                     RAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGE
                     LTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRS
                     EDEGLVTH"
BASE COUNT          325 a          407 c          425 g          220 t
ORIGIN      
        1 cggatggtgc cgggcgtcac ttccaactgg tactgaccac gcaggcgcag cgggcagaag
       61 aagcccggca gcaggccatt tccggcggga cggaaccgtc cgcttttcct gataccctgc
      121 cgggttacac cgaatatggc cgggacaacg gcatccgtct gtctgccgtg tggctgacgc
      181 acgacccgga atacccggag aatttacctg ccgcgccgct ggtgcgctat ggctggacgc
      241 cgcgcggcga actggcggca gtgtatgacc gcagcaatac acaggtgcgt agctttactt
      301 acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg ccggaaatcc
      361 gttaccgtta cgacagcgac gggcgggtga cagaacagct taacccggca ggcttaagct
      421 acacgtatca gtatgagaag gaccgcatca ccatcaccga cagcctgaac cgccgtgaag
      481 tgctgcacac gcagggtgaa gccgggctga agcgggtggt gaaaaaggaa cacgcggacg
      541 gcagcgtcac gcagagtcag tttgacgccg tgggcaggct caggacacag acggatgccg
      601 caggccggac aacagaatac agcccggatg tggtgacggg cctcatcacg cgcatcacca
      661 cgccggatgg cagggcatcg gcgttttact ataaccacca cagccagtta acgtcagcca
      721 ccgggcctga cgggctggaa atacgccggg aatatgatga atggggccgt ctgattcagg
      781 aaactgcccc tgacggcgat atcatccgct accgttatga taatccacac agtgacttac
      841 cctgcgcaac ggaagatgcc accggtagcc ggaaaaccat gacgtggagc cgttacggtc
      901 agttgctgag cttcaccgac tgttccggtt atgtaacccg ttatgaccat gaccgcttcg
      961 ggcagatgac ggcggtgcac cgcgaggaag ggctgagtca gtaccgcgca tacgacagcc
     1021 gtggacagtt aattgccgtg aaagacacgc agggccatga aacgcggtat gaatacaacg
     1081 ccgccggtga cctgaccgcc gtcattgccc cggacggcag cagaaacggg acacagtacg
     1141 atgcgtgggg aaaggccatc cgtaccacgc agggcgagct gacgcgcagt atggaatacg
     1201 atgctgcagg acgggtcatc cgcctgacca gtgaaaacgg cagccacacc accttccgtt
     1261 acgatgtact cgaccggctg atacaggaaa ccggctttga cggccgcaca cagcgttatc
     1321 accacgacct gaccggcaaa cttatccgca gcgaggatga ggggctggtc acccact
//
LOCUS       DAJVOY010000336         1318 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVOY010000336 DAJVOY010000000
VERSION     DAJVOY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.340"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..>1318)
                     /locus_tag="OQL90_004189"
     CDS             complement(<1..>1318)
                     /locus_tag="OQL90_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS1370732.1"
                     /translation="APCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQMKFAGYDVIII
                     EGKAKSPVWLKIKDDKVSLEKADFLWGKGTSATTEEICRLTSPETCVAAIGQAGENLV
                     PLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKRLNDYMMTELIG
                     ANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPPGNQNTVGFRTY
                     KSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGNTCVANFVHTTI
                     FPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYSKGVFKCVLPAE
                     EYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWNLGEEYWGYAKN
                     KLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL"
BASE COUNT          338 a          360 c          282 g          338 t
ORIGIN      
        1 tcaatggcaa gccggaacca ataaagttga tatgggtatg tgtcatgcag tcacggttga
       61 acatacagtt aacaatggaa ccgacctgcg ctgacgcttc attggcatgg tgaaccggat
      121 agccaaacgg cgaccagagt ttatttttcg cgtagcccca gtactcttca cccaaattcc
      181 agcgttctgc gatggcatat gaaccatcag ccaggtgact cagctcaccc acacgatgcg
      241 ccagacggta gtaaaaatct ttaatgaaat taacgtcacc cgcttccagt tgatcccagt
      301 gaatttccgc atactcttca gcaggcagaa cacacttgaa cacccctttg ctgtaacaat
      361 aagtaaagtc gcgatgcaac tgcccgtagt tacaccatag gccgtagtca tcgaacaaat
      421 tcagaccaac caagttacca atcacacggc catcgtcttt atcttcaaaa tctttcggac
      481 cgttcgggaa gatggtggta tggacaaagt tggctacaca agtgttacca cctgtgctgg
      541 gcacgccaaa ctctttcacc cgaggaatat tcatttgggt catacaacgg atcgggcaag
      601 agtggcagcc actcattttt actgtgtatt tctctgccgc cggacctaag tcaaaaacgg
      661 atttataggt acgaaagccg accgtgttct gattgcctgg cggaatttcc cccgtttcaa
      721 tcggaccacc ttcagccgcg ccccaaaaca gccctttacg tgctgtccaa cgtgacttgg
      781 gatctgaata ctctgcccac gattgcggcg tacttggcac gacatggttg ttattcgcac
      841 caataagttc agtcatcatg taatcgttga gacgcttcat ctcctgacga tcggcaatgt
      901 tgaccccttt cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc ataattgcgc
      961 cagttcccgc accgccgctg tggttacggc tattcaacat gccagagaga ggaacaaggt
     1021 tttccccagc ctggccaata gccgccacac aggtttccgg actggtcaat cgacaaattt
     1081 cttccgtcgt tgcgctcgtc ccttttcccc ataagaaatc ggctttttcc aggctaactt
     1141 tgtcatcttt aatcttcagc cataccggtg atttcgcctt cccttcgata ataatgacgt
     1201 cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc catatgggca tcgacaacta
     1261 aatttccttt ggtaaaagta gaaagtgagg tgatatttac gcgagaacta cagggggc
//
LOCUS       DAJVOY010000337         1307 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVOY010000337 DAJVOY010000000
VERSION     DAJVOY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.341"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(160..489)
                     /locus_tag="OQL90_004190"
     CDS             complement(160..489)
                     /locus_tag="OQL90_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCS1370733.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(661..>1307)
                     /locus_tag="OQL90_004191"
     CDS             complement(661..>1307)
                     /locus_tag="OQL90_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1370734.1"
                     /translation="DTALWRSGDVLLGSLLAMLFTGIWPQRAFIHWRIQLAKSLTEYN
                     RVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKETRIPKSIYEGIQTINRN
                     LVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANT
                     EKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          323 a          306 c          306 g          372 t
ORIGIN      
        1 ataacaatgg agttacagaa ctttttgcct tgctgcatac agaagatgtc tgtcgctaat
       61 ttcggtaagt tatcagatgg gaatgatgac ggtgatacac agaaaagaat caatgagcaa
      121 tgagggttgc caggcaaccc tcattgaata aaacaggaat tacttcgctt cgccgttttt
      181 catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg agagctcggc
      241 atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc tggcgatgat
      301 gccctggatt gcttcaacgc tcatccccgg cttgatgtaa tcgctcaggt tgtccagcag
      361 caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt cacgttgcag
      421 tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg aaggcttggt
      481 agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc aaccgctatc
      541 cgttagagcc aacattactc atagctgccg cagatgacaa tgcttttatc ccttttcact
      601 atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctgt agttcaggaa
      661 ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat gtgccgtttc
      721 catgttcagc cacacataac catagattgg tgtttccaca accttcaggt catggtggtt
      781 attgagcaac tgacgcagct cttccactgc atcgttcaat ttttccgtat tggcaaaaac
      841 cggctgcgga ttaccttcgt acaacgcatg aacaaggctc agcagtattt gctgcatcat
      901 gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg ggcgcgtggc
      961 ccagtacgca ttgatttgca actccagcat acaaaccaga ttgcggttaa tggtctggat
     1021 accttcatat atcgattttg gaatacgggt ttctttgctg gcgggcgcaa tcagcccacg
     1081 cattttcacg gcatcggtca ggagtttttg tagatggctt tccagacgtg ggcgttcgag
     1141 taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac ttttcgccag
     1201 ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca acattgccag
     1261 taaagagccg aggagcacat cgccgcttcg ccataacgcc gtatcaa
//
LOCUS       DAJVOY010000338         1305 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVOY010000338 DAJVOY010000000
VERSION     DAJVOY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.342"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            231..>1305
                     /locus_tag="OQL90_004192"
     CDS             231..>1305
                     /locus_tag="OQL90_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1370735.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIILADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIR
                     GIGYYGSKWRDSSATWTSQDRASH"
BASE COUNT          314 a          316 c          390 g          285 t
ORIGIN      
        1 ttgtgggcaa aaaaagcccg cgctgggaga cgcgggcggc aaggaataaa caataaaacg
       61 tgaagtaata tttcagctgg cgaataatac cccatagtag tcactctgcg caactgcgcg
      121 gtctttttcg aattgcgggc tgtcgtctct cttctgccat tgtcctgtaa cttccggact
      181 tcagcccgct cctcatttta ctcacaatat tatcccggcc gggaggattc atggctttta
      241 aacactatga tgttgtcagg gcggcgtcgc cgtcagatct tgcggaaaag ctgacacata
      301 aactgaaaga gggctggcag ccgtttggta gtccggtggc cataacccct tatactctga
      361 tgcaggcgat tgcagcagaa ggtgcagtaa tcagcgccac cagcgacccg gagtattact
      421 ttgttgtggt tctggcaggg cagtcaaacg gcatgtcgta tggtgaaggc cttccgctgc
      481 cggggacata tgaccgtccg gacccgcgta ttaagcaact ggcgcgtcgc agtacggtga
      541 caccgggcgg tgcagcatgc aaatataacg acatcattct ggcggaccat tgtctgcatg
      601 atgtgcagga catgagccgt cttaaccatc cgaaagcgga cctgtcaaag gggcagtacg
      661 gaaccgtggg gcaggggctg catatcgcca aaaaactgct gccgtttata ccggcgaatg
      721 cgggcattct gctggttccg tgctgtcgtg gtggttcagc gttcaccacc ggagctgatg
      781 gcacatacag tgacgcgagt ggtgcctcgg agaattcaac ccgctggggt gtggacaagc
      841 cgctgtataa ggaccttatc ggtcgaacaa aagcggcact ggagaagaac ccgaaaaatg
      901 tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt tggcggtacg ccggcaaatc
      961 atgccgcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg gatatggcag
     1021 gccagtgcgt cggtggctct gctggcggtg ttccctggat atgcggggac acgacgtatt
     1081 tctggaagca gaagaacgaa tccacgtacc agacggtgta cggcagctat aaaaataaaa
     1141 cggaaaagaa tatccatttc gtaccgttca tgaccgatga gaacggggtg aatgtgccga
     1201 cgaacaaacc ggaagaagac ccggacattc ggggtatcgg ttattacggt tcgaaatggc
     1261 gtgacagctc agccacctgg acgtcacagg acagggcgag ccatt
//
LOCUS       DAJVOY010000339         1300 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVOY010000339 DAJVOY010000000
VERSION     DAJVOY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.343"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OQL90_004193"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OQL90_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1370736.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
     gene            1117..>1300
                     /locus_tag="OQL90_004194"
     CDS             1117..>1300
                     /locus_tag="OQL90_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1370737.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAW"
BASE COUNT          399 a          224 c          245 g          432 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcagaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gagctaaata gctgcgcgga atagtaggct gtgtccctta atcatctgag
     1081 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     1141 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga
     1201 cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttccggt
     1261 gcaccatggc gcgatttacc cgaacgatat ggggcatgga
//
LOCUS       DAJVOY010000340         1278 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVOY010000340 DAJVOY010000000
VERSION     DAJVOY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.344"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..1278)
                     /locus_tag="OQL90_004195"
                     /pseudo
     CDS             complement(1..1278)
                     /locus_tag="OQL90_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          331 a          341 c          271 g          335 t
ORIGIN      
        1 cttgcatctt gtatcgcagc aatggctcca aaaggtaccg gtttaccatt tgcatttaac
       61 actctgaaga gaacgcgata acccacatga gtcacaaact ccgctttaac cactgcacca
      121 cgcgtgggaa ccatcgtaac gacggtatta tcaagttcta tattcgactt cgagaaatag
      181 ttatcgctga ttgctacccg attttcatca taagggttta gctgtgtctt gaccgtatag
      241 ccgcgccagt ccgtcgcagc accgcgcata ttatctattt ctaacccggc ggccccaggt
      301 gctttaacaa gagcaatagt ttcacctaac tcctgactta acgttacccc ttcgctgtgt
      361 gccagaattc cccctctgat accataattc agcctctggg aattttggct gtagttatag
      421 ctgccgttca ccgtgccata agagccatca taggtcaggg acatattacc gccaacctca
      481 tcatcatgac tgttatagga ctgcattaaa ttccatgaaa ggttatttct ttccagcgcc
      541 gtaccgcttg atgtgagcta ccggaattat cgttttgcga aacagaatag gtggcgtaac
      601 tattacccca ccagcccgat aaaggaacag ataaagagag catgtagatc tgctcactgg
      661 tgttatcact ggcatggttg ttccccgtcc cctgtctggc agtattggaa taactccagg
      721 agacgttcca cgagagacgc ttccagctac tgctgtagcc aaattgcatt aatcgttcga
      781 catcatcggt atgccagtat tgttgccggc ttaatgtcag gtataaattg ccataatctc
      841 tccccaacga ctgcgttagc gttccctcca cgcgactacg tcggttacct gtttcccaaa
      901 agtcttcagg gctgttgcgc cgcgatgccc actcattgag ggtatagtag ccctcggtag
      961 agtaacgata gccaaccagg cgcaagctag tgtctgtgtc gtcaaatcgt tttgaataga
     1021 gaaagcgata tgactgaccc gtttctgtat catcatggct aaaattcgca cgagcatgtg
     1081 tcacatcgaa agaaattgcg cccaaagcgc caagatcttt accgacaccc agagctgcag
     1141 aaccataatt ttcagcagcc tgcatacctc catataaagt gatcccgtgg gaaaaaccat
     1201 gaagtatttg cgcctgaagg acgtccggtg taaatccgtc ttcatcgcgc aattcaccca
     1261 cgctgacatc aacatctg
//
LOCUS       DAJVOY010000341         1242 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVOY010000341 DAJVOY010000000
VERSION     DAJVOY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.345"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            220..1167
                     /locus_tag="OQL90_004196"
     CDS             220..1167
                     /locus_tag="OQL90_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1370738.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
BASE COUNT          340 a          241 c          297 g          364 t
ORIGIN      
        1 aggtttagca ccgtcatgct cgtctactgt tgttctggtt gttggtgatg cggtggcgct
       61 tgcactctct gaattgaaaa aatttactcg tgctgatttc ggcttatatc atccaggagg
      121 tgcactcggc attaaagcaa attcataatt tcaaagtata aatacagtga ccggtcacgc
      181 catcttttgg ggtggcgttc attgaagtga gtgtcagcta tgaaaaatgt tctggtcacc
      241 gtccccagct tttcagcacg ctgtgtttcc gccagcaagt tgctaagaga gaataatttt
      301 aatcttatta ttaagaataa tgtggagcat ctgcttaaat cagaatccac tgcattacga
      361 gaatctattt gcgcggtcat cgccggaaaa gattgttatc aggcggacac gctatcattg
      421 ctgcctggtg tgaaaataat atcgagattc gggacgggga tagataatat tgatcttcgt
      481 gcagcacaac aatccggtat tgtagtcaat aatgcggttg ggattaattc aaatgctgtc
      541 gcggaattta tcattggact tatcttcgcg agcatgagaa atattcccgg cagctatcat
      601 gcgatgcaaa atggctactg gggtgagtcg catggctatg aattacaagg caaacggatt
      661 ggcatggttg gctacggcaa tataggtaaa accctggcaa aaaggttatc cggttttgat
      721 gttgagcttt tagcttatga caaacaaccc gattatcagg ttgctgacaa agttggagtt
      781 cagtttgtat caattgaaga tatctttatg cagtcgcatg tcatcattgt tcttttgcct
      841 ttttctcctg agctggagaa cttcattagt cataaatatt tgtcgatgat gcgcaatggt
      901 gctctaatca tcaatgccgc aaggggaaag ttgctggatg agggcgcttt actacaggtg
      961 attgaagaac gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc gttggcgcaa
     1021 tttagcccac tgctacatgc caaaaatatc attacaacgc cgcatattgc ggccgccacc
     1081 gttgagtcat accagcaaac cggtacacac gttgcccagt cgataattga ttacttcgca
     1141 ggaagggaga taaaaaacgt gttgtgagtt cagcgaacga agtttttaaa tacaaaaaaa
     1201 ccgcaatttg tggaacacat tgcggtttat cagcattcta ag
//
LOCUS       DAJVOY010000342         1226 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVOY010000342 DAJVOY010000000
VERSION     DAJVOY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.346"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..80)
                     /gene="cobS"
                     /locus_tag="OQL90_004197"
     CDS             complement(<1..80)
                     /gene="cobS"
                     /locus_tag="OQL90_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="catalyzes the formation of adenosylcobalamin from
                     Ado-cobinamide-GDP and alpha-ribazole; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1370739.1"
                     /translation="MSKLFWTMLSFITRLPVPRRWSQGLD"
     gene            complement(77..621)
                     /gene="cobU"
                     /locus_tag="OQL90_004198"
                     /pseudo
     CDS             complement(77..621)
                     /gene="cobU"
                     /locus_tag="OQL90_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            complement(755..1192)
                     /locus_tag="OQL90_004199"
     CDS             complement(755..1192)
                     /locus_tag="OQL90_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCS1370740.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
BASE COUNT          338 a          339 c          245 g          304 t
ORIGIN      
        1 aaatccagtc cctgggacca gcgacgcggt acaggcagac gcgtaataaa agagagcatc
       61 gtccaaaata atttactcat ttgattttta ctccaatacc cgaaaccacc aaccatactt
      121 catttgccgc agcggccagc tgctgattta cccgcccggc aatatcacga aaatgtcgtg
      181 ccagacgact ctccggcaca atccccattc ccacttcgtt agtcactaat acaacctttg
      241 cgggacaacg ttggcaggca gcaatcaacg actgaatctc agcattaatc gcctgttcca
      301 tcgcctgata atcccattca tcagggtctt tatcgccgcc ataatcaaac aacagattag
      361 tcaccatggt ggtaacgcat tccagcaata cagcctcatg cgggttaatg tctgcatgaa
      421 ttaattcatc aagatgttgc cagcgctcca ctgtgtgcca gtgcgtcggg cggccttgcc
      481 gatgatgttc tatccgtgca gccatctcat catcaaggat ttgcgaggtg gcgtatacag
      541 aacctgtgaa gagtccccaa taagcgcctc tgcgtggcga ctcttcccgc tccgtatgcc
      601 ccccgtcacc gaaatcatca taccgactcc tgattcaatt gtatgatggt attcattgaa
      661 cagaacaatg gatcccccac cgttgctgac cgcaaaacat ttacgcgatt aatactgcgc
      721 gtaatataat taaaaatcca gcattctcaa tccattacga aagaagatgg cattgcggaa
      781 tagccagact tcctcgtgtt aacgtccgac aaaatgcgtc attgacagcg gtttctctgg
      841 aatgacagat ttcattcaga taatggatta atgctacagc cccaccagcc ggaccagtca
      901 gcaaacgggc attgtgttct gcaatcaaaa ttaccgtcgt tccctcagaa tgagcgagcc
      961 attctggtac ggatatctcg ctttcagctg ctatcaatag cagcactgaa taatcgctat
     1021 acgaatctga taacgattgc acaggcccac catcaagagg ctggaaatct tgctgccagt
     1081 cctcaccaac aaaacaggtt attggcaata tatcagccag atgtttcgcc agatccacat
     1141 ctccagataa gaggcaaaaa agagctatcg attcgggttt tatcgttttc atgcctctcg
     1201 ccctccactt aatttcctgt aatggc
//
LOCUS       DAJVOY010000343         1209 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVOY010000343 DAJVOY010000000
VERSION     DAJVOY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.347"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            4..567
                     /locus_tag="OQL90_004200"
     CDS             4..567
                     /locus_tag="OQL90_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1370741.1"
                     /translation="MCLAVSGAPAWASEHQSTLSAGYLQPHTDMPGSDDLKGINVKYR
                     YEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAY
                     AMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESV
                     AIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            631..>1209
                     /locus_tag="OQL90_004201"
     CDS             631..>1209
                     /locus_tag="OQL90_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1370742.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVVQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAENARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQA"
BASE COUNT          274 a          294 c          397 g          244 t
ORIGIN      
        1 gccatctgtc tggctgtatc cggtgcgcct gcatgggcgt ctgaacatca gtccacgctg
       61 agcgccgggt atcttcagcc ccatactgat atgccaggca gcgatgacct gaagggcatt
      121 aacgtgaaat accgttatga gtttacggac acgttggggc tggtgacgtc attcagctat
      181 gccaacgctg aagatgagca aaaaacgcat tacagcgata cccgctggca tgaggattcc
      241 gtgcgtaacc gctggttcag cgtgatggcg gggccgtctg tgcgcgtgaa tgaatggttc
      301 agcgcgtatg cgatggcggg cgtggcttac agccgtgtgt cgaccttctc cggggattat
      361 ctccgcgtaa ctgacaacaa ggggaaaacg cacgatgtgc tgaccgggag tgatgacaac
      421 cgccacagca acacgtctct ggcgtggggg gctggcgtgc agtttaaccc gaccgaatcc
      481 gtggccattg accttgctta tgaaggttcc ggcagtggcg actggcgcac tgacggtttc
      541 atcgtgggtg tcggttataa gttctgatta gccaggtaac acagtgttat gacagcccgc
      601 tggttcaggc gggctttttg tggggtgaat atggcagtaa agatttcagg tgtactgaaa
      661 gatggggcgg gtaaacctgt cgtaaattgt gcgattgaac tgcgggccag aagaaccagt
      721 ccgaccgttg tggcacacgt tgttgccact tgcgtgacgg acaataacgg tgcttatgtg
      781 attgaggctg agccggggta ttacgaggtt gcgcttcact gtaacggctg gcagccaacc
      841 cgtgtcgggg atattgatgt ggcaccgact gatgcaccgg ggacactgaa cgcgtttctg
      901 aatgcaccaa aggatggtga tttacgtccg gaggtgatga agcgctttga ggaaatggtg
      961 gtgcaggcgc agcagagcgc cggggccgca gccggaaacg cacagcagac ggcgcaggat
     1021 gtggcggcag ccgcaacggc ccgtgatgat gcacaacgtt ttgcggagaa tgccagacag
     1081 gatgcaaccg tcacagctga ggacagaaag gccactgcgg aagatgtgac aagcacagga
     1141 gcaaatgcag ccgcagccgg acagagcgca caggatgccg caggttatgc ccgcgcagca
     1201 gaacaggcc
//
LOCUS       DAJVOY010000344         1205 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVOY010000344 DAJVOY010000000
VERSION     DAJVOY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.348"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..695)
                     /locus_tag="OQL90_004202"
     CDS             complement(<1..695)
                     /locus_tag="OQL90_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1370743.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
     gene            complement(1011..>1205)
                     /locus_tag="OQL90_004203"
     CDS             complement(1011..>1205)
                     /locus_tag="OQL90_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCS1370744.1"
                     /translation="TILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHF
                     ENVKTKVQGTVVMFSASGKK"
BASE COUNT          351 a          205 c          253 g          396 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac gttaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagttct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatatcctg
      721 ataaaatgtg atactgggaa ataataagct aaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa tgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagcag aaaacattac tacagtacct tgaaccttgg ttttaacatt ctcgaaatgc
     1081 tctgagagta tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc
     1141 tgattgtaaa tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga
     1201 attgt
//
LOCUS       DAJVOY010000345         1180 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVOY010000345 DAJVOY010000000
VERSION     DAJVOY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.349"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..174)
                     /locus_tag="OQL90_004204"
     CDS             complement(<1..174)
                     /locus_tag="OQL90_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HCS1370745.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
     gene            complement(207..428)
                     /locus_tag="OQL90_004205"
     CDS             complement(207..428)
                     /locus_tag="OQL90_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCS1370746.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            554..1123
                     /locus_tag="OQL90_004206"
     CDS             554..1123
                     /locus_tag="OQL90_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HCS1370747.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFECKIEDIEIIGKIISLTVKYVR"
BASE COUNT          343 a          213 c          275 g          349 t
ORIGIN      
        1 cagccagatt tctggtgcgg ttaataaatg aggctgagct gggttcagct tgttccgcag
       61 aatctgcaca ttcatgcctg cacgttctgc cagttgcacc agattgtggc gcagtgcaaa
      121 tgcacgacag gcttcatcaa aatgtggatg tttggaaact tggtaatcaa acatggtcaa
      181 tgcctctgat gtatttcaga atcgaactaa ttaaggttta gattgcattc tgaaagcgca
      241 tcaacggtca ttgctgctat gttgatcatc actttttcgc gttttttatc tttgcgcaga
      301 cggtgacgga taaggcgtcc atcagccaac atgtcattga tggtatcgat ggatagccct
      361 gtcagctcgc tatagcgttc aatagtcaca tgaggcgtgg taagagtgat tgaaatgtta
      421 ggtctcatga tgcaacattc ctcgtttaat gatgattaat caggacgaat acggatcgtt
      481 tgtattttgt gaacaccata aacatacgat tgcacagtga aatcgtcaag ataaaagttc
      541 acttggagtg accatgaatt tggagaaagg cggacgaggc gccatagagc gcatggtaga
      601 agcttatgga ttcaagactc gacaggcgtt gtgcgatcat ttaggaatct ctaaaagtac
      661 actcgccaca cgctacatgc gtgactcatt cccagcagaa tgggtaatcc agtgcgccct
      721 tgaaacgggc acctcgctta attggctcac aaccggacat ggttcaaagc aaacttcagg
      781 taatacaaat actatggaag ttgctaaata tgtattatct gatggtgcct tgcgtgaaga
      841 cggtttttat atttttgata agggatttct accctctacg tttaaaaaac cttttgtcat
      901 cacagataac aattctgaat ttatttgtga taaagaattt gatgacatac gtgatggtaa
      961 atgggtaata agtattgatg gcgaagtaac gatccgtgac attactcgtt tacccggtgg
     1021 aagaatcttc gtcgagggtg gaaacagagc cttcgaatgt aagatagaag acattgaaat
     1081 aattggtaaa attataagtt taacagttaa atatgttaga taataccggg aggaaactat
     1141 gcttggtaaa gtattttttg tggttttatc atgctctttg
//
LOCUS       DAJVOY010000346         1179 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVOY010000346 DAJVOY010000000
VERSION     DAJVOY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.350"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..925
                     /locus_tag="OQL90_004207"
     CDS             <1..925
                     /locus_tag="OQL90_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCS1370748.1"
                     /translation="RRQDFLTFFGNREPCLIGMEACGGSQHWARELTKLGHKVRLLQA
                     RFVKAFVMGNKNDVMDARAIWMAVQQPGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQ
                     INALHGTLLEFGETIHKGRAAMEREFPEALERMKERLPPYLIMVLENQYNRLNELDSL
                     IEDIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGREFAAYVGLV
                     PKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKKRRPASVAI
                     VAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          331 a          266 c          337 g          245 t
ORIGIN      
        1 gcgtagacag gattttctga cgttcttcgg caaccgtgag ccatgcctga ttggtatgga
       61 ggcctgtgga ggttctcagc actgggcacg ggaactgaca aaacttggtc ataaagtccg
      121 gttgttgcag gcccgcttcg ttaaggcatt cgtcatgggc aataagaatg atgtgatgga
      181 tgcccgggct atctggatgg cggttcagca gccgggtaaa gaaatcgccg taaaaacaga
      241 agaacagcag tcggtactgg ttctgcaccg tacccgcatg caactggtga agttccggac
      301 cgcacaaatt aatgccctgc acgggacgtt actggagttt ggtgaaacca tccacaaagg
      361 ccgggcagcg atggagcggg agttccccga agcactggaa cggatgaaag agagactgcc
      421 accgtatctc attatggttc tggaaaacca gtacaaccga ctgaatgagc tggactcact
      481 gatagaggat attgaaaaac agcttaccag cgtggcgagg cagaatgaaa cctgtaagcg
      541 gttgctggat attcctggcg ttggaccact tattgcgacg gcagcggtgg ccaccatggg
      601 ggaagcatca gcgtttaaat cggggcgaga gttcgccgca tatgttggtc tggttccaaa
      661 acaaactggc tccggaggga aagtacgtct gctggggata agcaaacgtg gtgacactta
      721 tctcaggaca ttatttatcc acggtgcaag agcggtggca ttagtagcta aagagcctgg
      781 cccgtggata accgaactga aaaaacgtcg tccagccagt gtggcaatcg tcgccatggc
      841 aaacaagctg gcacgaacag tatgggcgat aaccgcccat gaccgtaagt atgacaggaa
      901 ccacgtcagt atcagaccat attaatcgct gataccatta aacaatgaac tcttaacaaa
      961 agggtgaatg ctgaaaggtt gctatggcgg ccagagtgat gacaaagaca ggtaagaccg
     1021 tgactcacta aacctgaaca gtattttggg cttgaagtcc gccgtgaaaa taaggggtga
     1081 gtcggcgaat tacatagggg ctcgcagcgt tacggctgca ataaagccgg atataaagct
     1141 gcaacctacc cgtcatgtca aaacaatgga tgccttgca
//
LOCUS       DAJVOY010000347         1174 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVOY010000347 DAJVOY010000000
VERSION     DAJVOY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.351"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            16..174
                     /locus_tag="OQL90_004208"
     CDS             16..174
                     /locus_tag="OQL90_004208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370749.1"
                     /translation="MYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQ
                     AYDPARSK"
     gene            174..764
                     /locus_tag="OQL90_004209"
     CDS             174..764
                     /locus_tag="OQL90_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCS1370750.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            751..>1174
                     /locus_tag="OQL90_004210"
     CDS             751..>1174
                     /locus_tag="OQL90_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1370751.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRFATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAF"
BASE COUNT          297 a          252 c          347 g          278 t
ORIGIN      
        1 gtgccagagg ggcgtatgta tctgtccaga atcagtgagg ccatcagttt gtctgctggt
       61 gaagtggccc accggttaat cgtcccttca tcggatattg acctggggga gaccgagctt
      121 ccggtgctcg gcgagatcac ctggcaggct tatgacccgg caaggagtaa ataatggatt
      181 cgttacagga tgattataca aagctgctgt acggactgat gcagccgggg cctgcatggt
      241 cagataccga cggtgtactt gacggtctgg caccatcgct tgtgcgtgtt caccagcggg
      301 ctgatgaact ggtgattgaa atcgatcccg gtcagtccac agagctgatt gaacgttatg
      361 aagaattgta tggtttacct gattcctgtt cccctgtcgg aacccagaca ttacgccagc
      421 gtcagcaacg tcttgaagca aaagccaatg tggctggtgg cataaatgag cagttcttcc
      481 tggatcagct tgaggcgctg ggatataccg gcgtaacgat cgaacagttc cagcacctgg
      541 atgcaagccc cgatccggaa tggggagatc gctggcgtta tttctggcgt gtgacgttgc
      601 cggtggatgc cggtgctcag tggcagacat gcacggacgc ctgcaacaca ccgatccgga
      661 cgtgggggga tacggttgcg gagtgcgtga ttaataaatt atgtccgtca cacaccgtcg
      721 ttttattttc ctatccagat gaggatgaag atgcacagga ttgatacgct gactgcggta
      781 aaagataagt ttggtccggg taagaacgga tttactgacg gaaatcttcg cacaggacgt
      841 tttgctacct ggctgaacag tgccatgtgg aatgccattc aggaggaaat ctgtggtgtc
      901 attgagaagg ccgggataga actgaataaa gaagaacacg accagctata taaggctata
      961 ttattgctgg tgggcggtgc aattaacgaa gaggcattgc tgataaaaaa taacctttcg
     1021 gatgtggaag acagggatga ggctgttgaa aacctcggat taaaacccac ggtggataag
     1081 gcaaaaaatg ccgttcagcg tgatggcgac accatgaccg gagagctgaa aatccgtggt
     1141 gttaatgcgc tgaggatttt caacgaagct tttg
//
LOCUS       DAJVOY010000348         1174 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVOY010000348 DAJVOY010000000
VERSION     DAJVOY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.352"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..862
                     /gene="pocR"
                     /locus_tag="OQL90_004211"
     CDS             <1..862
                     /gene="pocR"
                     /locus_tag="OQL90_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HCS1370752.1"
                     /translation="FAQATSLAVVVVNIHGDEISELFNFTPFCQMMRQHPQLSTRCRM
                     SDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGFVLCGQVRLRNDYVDLVDI
                     LNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVENCLKKQLNFVVIKENKSQ
                     SDSVRQTRAPSPHDNKMKKALRYIDAHLSDDLRLEDVASHVYLSPYYFSKLFKKYHGI
                     GFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYFCKVFRQTYQVTPQVYRQQ
                     VSENAAMETV"
     regulatory      939..1114
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          316 a          245 c          295 g          318 t
ORIGIN      
        1 ttttgcgcag gcgaccagcc ttgcggttgt cgtggtcaat attcacggcg atgaaatttc
       61 cgagctgttt aacttcactc cattttgtca gatgatgcgg caacaccctc agctaagcac
      121 tcgatgtcgc atgagcgatc gttgtggggg cctggaagcc tctaaatccg atcagccttg
      181 tatttatcgc tgtcatgccg gattaaccga tttctctatt ccactggtaa tcgccgggca
      241 tttagtcggt tttgtattgt gcgggcaagt tcgtctgcgt aatgattatg tcgatttggt
      301 cgatattctg aatgtcgatg attgctggca ggcagatccc gaattactta atgaatttcg
      361 caaagttcct gagatggatt actcaagagt tatggcctct gctgatttgt tgaagttgat
      421 tgtagaaaat tgcctcaaaa aacaactcaa ttttgtggtt attaaagaga acaaatctca
      481 gtcagattcg gtgcgccaga ctcgtgcgcc tagtccccac gacaataaaa tgaaaaaagc
      541 tttacgctat attgatgcgc atttatcaga cgatttgcgt ctggaagatg tcgcctcgca
      601 cgtttatcta agcccttatt atttcagtaa gttattcaag aaataccatg gaatcgggtt
      661 taatgcgtgg gtaaaccaac aacggatggc cagcgcgcgt gagttactgt gccatagtga
      721 ctggagcatc gccagcattg cgcgtaattt aggtttttcg caaaccagtt atttttgcaa
      781 agttttccgt cagacttatc aggttacgcc gcaggtgtat cgacagcaag tgagtgaaaa
      841 tgcggcgatg gaaacggtat aaaaaaaggg aaggtttaaa taaacgctgg aatgaataat
      901 taacgttatt tacgtgttat gggaaaagta aaatcagctc ataacatacg ctgacaggtg
      961 tggcacattt gtgcctggga agagagtgaa aatccctcgc agcccccgct gctgtaatgc
     1021 tgacaaaccc gaaaatgcca ctgatcgcga gattgggaag gtcgggagag gatgacgcta
     1081 agccagaaga ccagccagtc agtcaatacc aacaacttcg gtgggaagcg ggttgcccgg
     1141 aagcgtccgg tagagtacag gcgcgtcttt atac
//
LOCUS       DAJVOY010000349         1168 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVOY010000349 DAJVOY010000000
VERSION     DAJVOY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.353"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(279..566)
                     /locus_tag="OQL90_004212"
     CDS             complement(279..566)
                     /locus_tag="OQL90_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1370753.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(641..784)
                     /gene="mgrB"
                     /locus_tag="OQL90_004213"
     CDS             complement(641..784)
                     /gene="mgrB"
                     /locus_tag="OQL90_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1370754.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            943..>1168
                     /locus_tag="OQL90_004214"
     CDS             943..>1168
                     /locus_tag="OQL90_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370755.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIV"
BASE COUNT          378 a          249 c          230 g          311 t
ORIGIN      
        1 taaaataaga aaaattgatg aatgagcaaa aaaatcaaga gagaaacgtt tcgctaataa
       61 ataataccac gcttaatcac aaatccgatg taaacatcct aataaattaa tgaggaaaat
      121 atctttttct gatatcccgt tacgcaaata aataatatga taattatttg aagtcagacc
      181 agagttttaa tttgaagcag ttgcaccatg acagtgcgta taatcaggat gaattgagta
      241 acccttcaac gacggggtta ctcaagattg aggaaggatt atcgttcggc gaccaggcga
      301 ataatatctt cgtcatcaac cgatttttga ctctcaatgg tctttttatc tgctttctct
      361 ggttcgtttg cttcgcacaa acgacgtaac agtgcatttt gacgtttttg ctgatccagc
      421 aacgcctcaa gcagttcaat ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac
      481 cacaagatga gcccgataag aagaactacc accgatacaa ccaaagacgc aatgttcatc
      541 acgcctgaat ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca
      601 ctgctggaga ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt
      661 taatggcaca aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct
      721 gcgcccaaag cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt
      781 tcactccaat ctccgcttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca
      841 gcgcctaact atgtcgatat tcgtgctttt tcggaatgag tagcttgctt cagcgcatat
      901 tgccgctacg attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc
      961 acagttatgc tgatagcact ggtatggata ggattattac tcagcggata tggcgttttg
     1021 attggaagca aagagaatgc cgcaggatta ggtttgcaat gtacctatct gactgccaga
     1081 ggcaccagca cggtacaata cttgcacact aaaagtggtt tcttggggat aacagattgc
     1141 ccactgttac gtaaaagcaa tatcgtgg
//
LOCUS       DAJVOY010000350         1167 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVOY010000350 DAJVOY010000000
VERSION     DAJVOY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.354"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..314)
                     /locus_tag="OQL90_004215"
     CDS             complement(<1..314)
                     /locus_tag="OQL90_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370756.1"
                     /translation="MADKAILWALISASTKEERKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IK"
     gene            complement(1022..>1167)
                     /locus_tag="OQL90_004216"
     CDS             complement(1022..>1167)
                     /locus_tag="OQL90_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1370757.1"
                     /translation="LSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNK
                     ENP"
BASE COUNT          338 a          240 c          197 g          392 t
ORIGIN      
        1 tcttttattt tatttactgt ttcaatgcaa tcagcggcaa gttggagtgg atttagattt
       61 ttcagatatg gtcttatgat ctttccctta cttaataaat agcatgtata ggattcggaa
      121 agtccagcat ctattttgta cctcattata ttagataagg atgtgagaaa ttccttgtta
      181 tcattcgcag ccatatatgc cagtccgagt tcagcctctg cggccttaca ggcaaaatag
      241 cttagtgaac acgcttttcg ttcctcttta gtactggctg atattagtgc ccataatatt
      301 gctttgtctg ccaaatctaa ctctctctga aattgtcttg cagtaactgc actcgcagaa
      361 atagagaaaa aaatacaatt gttaataacc ttgccatatt tttttagtcc gtaactgtta
      421 atgtgctatc tggctctgaa aatattaact catttaattt ttgcatgtca ttagataaac
      481 cagttataca accctcggaa cagaatcgag gtcctaaaga cggaccaaaa gcgtttaaac
      541 aactatgtat tgaaaatcca ttgcgattat aagttcctgt attaactgac ggtactaatc
      601 gtatgccgtc aggttttcta ttgcttgctc ccgggtcatt aacattccaa ctccataggc
      661 cttgaggtat tggccatcta tttataaatg tgtacaacgg ggattatttt tacattgcat
      721 attattacca gaagcgactg gaatactaag cacatcataa aaattatcat cacatggtat
      781 atctgaaaca cacgaaagca tacctaaagt tatgctgtat aaacaggtgg ctaatcccaa
      841 ggggatcgat tccagaaatc gggttcaacg gatactgata aaaattccac ctaccttcca
      901 gcccaatcgg agcctgagtg ttataccgcc cctgctgcgg ttaatgttac cgataatagt
      961 tatagtacag ccctgattca tcatcatact actgctgcgg cagtctgatg aactattgct
     1021 tttacggatt ctccttattc ttccttctat ccattcgctt tagctttttg agctcttgaa
     1081 gtttgctctt tgcatcaata ttaccgttat ttacagcgaa ttccatccaa taaatagctc
     1141 tattcaaatt ataatattct gataatg
//
LOCUS       DAJVOY010000351         1154 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVOY010000351 DAJVOY010000000
VERSION     DAJVOY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.355"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..265)
                     /locus_tag="OQL90_004217"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="OQL90_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OQL90_004218"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OQL90_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCS1370758.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          337 a          284 c          176 g          357 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactctt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tgttttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gagtctaaaa
     1141 agtgatccac ttac
//
LOCUS       DAJVOY010000352         1148 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVOY010000352 DAJVOY010000000
VERSION     DAJVOY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.356"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..315)
                     /locus_tag="OQL90_004219"
     CDS             complement(<1..315)
                     /locus_tag="OQL90_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1370759.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNP"
     gene            complement(327..>1148)
                     /locus_tag="OQL90_004220"
     CDS             complement(327..>1148)
                     /locus_tag="OQL90_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1370760.1"
                     /translation="YTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWSNLLSDE
                     NPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNP
                     VINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDL
                     AYLIKFDGNYKDGMTVWLFSCNTSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNG
                     KLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          305 a          262 c          219 g          362 t
ORIGIN      
        1 tggatttgta tctgataaaa agaccccata attaaactgc gctgtaacat tcccctgaga
       61 tgctgatctt tccaagaatc gcaattgttt atcaatatta tttttagtaa agcagtaata
      121 ttgatatagt cgaaatgatg cctctgaatc accatgttgt gatttatact tcagtttcat
      181 cagcttttct tcacttaaat cataggtcat tgctgtagat cccatcacac tttcgttttc
      241 ttttattttc acatcagcac catgcaattg atcaataaga gaaaaataaa aaaatagtaa
      301 caagcatgct ttcatacatc tacaacctat cttatatcac tgggtttttc tggcgtacct
      361 tgcatattag aaatggggtg cccagatggc ccatatgtta tccaattacc aaggtctttt
      421 gttgttatag ccattagatc cttatttgaa ttaaggttcg ttggtgctgt tagcactgta
      481 tccattttta acttaccatt agttcctcgc ccccaccacg tccatagcgt gtcaggtcct
      541 attacatttg tatgtaactc tttagctaat tggctagcaa atgaattttg tcctttactt
      601 gtattacaag aaaataacca aactgtcatc ccatctttat aattcccatc aaatttaatt
      661 agatatgcta gatctttagc tgtcatgata ctgcgcgttg cggattcaat agatgtgggg
      721 gtaccatgcc ccccgattgt gaaaacgcct gggatattaa tctcatcagc tacagaatgg
      781 ataagatcac tttcggggta taaatttata tcaaccaaac cttgcggatc tacatttatg
      841 accggattca acggatactg ataaaaattc catcccccct tcagcccaat cggatcctga
      901 gtgatatacc gcccctgcag cgggtcataa tagcggtggc ggttgtaata caggccggac
      961 tcctcatcat actgctgacc cggcaggcga atgagctgct gcagatgatg cgggttctca
     1021 tcactcagca ggttgctcca ttcatcatat tctgcgcacc acgctgttgc cccttccgtg
     1081 ctgatgagcg ccagcggcag gccgcgatgg tcgcagtggt acaggtggat ttttcgcgcc
     1141 ggcgtgta
//
LOCUS       DAJVOY010000353         1125 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVOY010000353 DAJVOY010000000
VERSION     DAJVOY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.357"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..427)
                     /locus_tag="OQL90_004221"
     CDS             complement(<1..427)
                     /locus_tag="OQL90_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCS1370761.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIE"
     gene            797..>1125
                     /locus_tag="OQL90_004222"
     CDS             797..>1125
                     /locus_tag="OQL90_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370762.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
BASE COUNT          301 a          277 c          286 g          261 t
ORIGIN      
        1 gttcgatttt ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg
       61 actggctacg ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag
      121 taaacagacg accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg
      181 catcttcaaa gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg
      241 ccgatgcttc gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt
      301 gtacacgcag tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa
      361 ggggttgtga ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc
      421 tcagcatgac cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa
      481 caaattgggt ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg
      541 ctgacaaccg ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac
      601 aggatagtga gccagtatct actgggtgtg tccaaggcga atgtcctctt agaatgatcg
      661 tgcgcgcatg ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca
      721 atagttgaac ttcacgcttt ttggtactcc tcaaggtcgc taacaggata aaaaaatccc
      781 acatccagga cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa
      841 tctcaaacgg atttttctca ccctcacctt acttcccttg ttttctgttg ccgctgatga
      901 ttgcgcactc tcagatccga cgctgaccgt acaggcgtat accgttaatc ctcaaacaga
      961 gcgggtgaaa atgtactggc aaaaagccaa tggcgaagcg tggggaacgt tacatgctct
     1021 gctggcggat attaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga
     1081 tgaaagctat gcgccgctcg gtttgtacat cgaaaacggt cagca
//
LOCUS       DAJVOY010000354         1086 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVOY010000354 DAJVOY010000000
VERSION     DAJVOY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.358"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..447
                     /locus_tag="OQL90_004223"
     CDS             <1..447
                     /locus_tag="OQL90_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370763.1"
                     /translation="LPISAADKRRFYYYFQEKNTPNIERFFVFDSSEYRYALNMREVV
                     FHQFLSDGLRPIVDEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQLNAFFDA
                     LDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDVLEPVEDDDE"
BASE COUNT          279 a          212 c          261 g          334 t
ORIGIN      
        1 ttgcccattt cagcagcgga taagcggagg ttttattatt attttcagga aaaaaacacc
       61 ccaaacatag aacgcttctt cgtttttgat tcttccgagt atcgttatgc gttaaatatg
      121 cgtgaagtgg tatttcatca gttcctttct gatggtctgc ggccaatcgt tgacgaagac
      181 gattatttta atgtccatat cacgctggta aacggcggac cagtgattcc actgagtgtt
      241 gagccagatg cgccgcaaaa cgaagagacg gatgatatcg gccagttgaa tgcatttttt
      301 gacgcgctgg attgcgagcc tgaaaccacg gatcgtttta tgataaccga cgaagatgga
      361 gaagatgctt tcattcgtat tggttctatc gctatggtgc gagttgcgct tgatgtgctg
      421 gaaccagtgg aagacgatga tgagtagtat tgaatggatt gatttcttaa gaaatggtac
      481 tgagtataca tgtgagtata caaagtaact tcgtattttt aaaaatccat tgatttcata
      541 ttgttgagtc tggaatcgcg gtgcggcctt cgcaccacac cgtatgcaaa tcgagtcgct
      601 caagagcggc tttttttgtg tctgaaatca cccatatccg tgcagtatat aggcctgcca
      661 gaaaagcagt tcagtattct tcattttgta gtcgatggtc ccaagtttgc tctggctgga
      721 agctgcatcg tccacattac gtgggcaggg tggtttcgct atgctacttg aacgttttga
      781 ccatgtggct caggtagcta ataccaaaag acgtcatttt gtctccacgc ccaccttggt
      841 gaacattcaa taaatagttt cggtcgagag cagttatgta gggctagcag acgagatgcg
      901 cagacatctt gttgaagaca attttactga ccacctgaga acgctattct accgaataat
      961 atttattatc ttggcgaata atgatgatta cctgtgtaac tatggtttta tgctgatggg
     1021 accggcagta caatttctgt agtgtactcg caatttcagg tctctggcgt ttattctggt
     1081 gcaaag
//
LOCUS       DAJVOY010000355         1060 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVOY010000355 DAJVOY010000000
VERSION     DAJVOY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.359"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..489
                     /locus_tag="OQL90_004224"
     CDS             <1..489
                     /locus_tag="OQL90_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCS1370764.1"
                     /translation="PIGLKGGWNFYQYPLNPISEIDPQGLNPLILGVVALMSCGLFSS
                     GGVIQSGQQRREQYGRKVNPEADKLTDEHKSGVNPGGNCSPQNLSDLQNEKNRLCNQS
                     RACAPQMLRKEILRRYEINLACASVRKQINNQCFGGGDKAHMEEENKAYKTAADCSGL
                     IK"
     gene            486..989
                     /locus_tag="OQL90_004225"
     CDS             486..989
                     /locus_tag="OQL90_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370765.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDIIGLDELDIVYNF
                     FQKKQWNEIANNLKIKDDSYALELGITFLPEKVFCYYIPLYIYVSLFNKNDFWVFESD
                     FIQQYLCPEYRDYDDFLNFVFNFSDIQLSIIAQFMSYESDAGFFYASKACMDFWEDYS
                     PLLHKKI"
BASE COUNT          352 a          154 c          218 g          336 t
ORIGIN      
        1 ccaattgggc tgaagggggg atggaatttt tatcagtatc cgttgaatcc gatctcagag
       61 atagacccac aagggttaaa tccgttaatt cttggtgttg tagcactgat gagttgcggt
      121 ttgttttcat caggtggtgt tatccaaagt gggcaacaac gcagagagca atatggacgt
      181 aaagttaatc cagaagccga caaattaaca gatgaacata agtcaggtgt aaatcctgga
      241 ggtaactgta gtccccagaa cctgagtgac ttacaaaatg aaaaaaacag attatgtaac
      301 cagtctagag cttgcgctcc tcagatgctg agaaaagaaa tattgagacg gtacgagata
      361 aatttggcgt gcgcttcggt cagaaagcag attaataatc agtgctttgg tggtggagat
      421 aaggcccata tggaagaaga gaataaagca tataaaacag cagctgattg cagtggctta
      481 ataaaatgaa aaaaataatt ttctctaacg agagtgcagt ctatgatgaa cttatgattc
      541 atttcccatg ccagccatta cctcatattt caaatgatat tatagggctg gatgaactgg
      601 atattgtata taattttttc caaaagaaac aatggaatga aatagccaat aatttaaaaa
      661 taaaggatga ctcttatgct ttagagttag gaattacatt cctccctgag aaagtattct
      721 gctattacat ccctctgtat atttatgtct cgttgtttaa taagaatgat ttttgggttt
      781 ttgaatccga ttttattcaa caatatttat gtcctgagta tcgggattac gatgattttt
      841 tgaattttgt ttttaatttt tcagatatac aactatctat tattgcacag tttatgtctt
      901 atgaatcaga cgctggattc ttttatgcaa gtaaagcttg tatggatttt tgggaagatt
      961 attcacctct attacataag aaaatttaat cgtaaaaggt ataaatggtt taataaaaaa
     1021 cttgtctact cgttggagta gatgaaaaat aagatgacgt
//
LOCUS       DAJVOY010000356         1041 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVOY010000356 DAJVOY010000000
VERSION     DAJVOY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.360"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            103..621
                     /locus_tag="OQL90_004226"
                     /pseudo
     CDS             103..621
                     /locus_tag="OQL90_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          303 a          242 c          221 g          275 t
ORIGIN      
        1 actctgacca caatctgccg gttacgccga atctgctgaa tcagaacttt acgcccacag
       61 cacctaatca ggtctgggtc gcagattcag ttgtccaggc cttccgaaat caaccgactg
      121 aaggcgctgg tcgcgaaact gcagcgtatg cagttcggta aaagctcaga aaaacttcgc
      181 gcaaaaaccg aacggcggat acaggaagca caggagcgaa tcagcgcact tcaggaggaa
      241 atggcggaaa cactgggtga gcaatatgac ccggtactgc catcttccct gcgccagtct
      301 tcagcccgta aaccgttacc agcctcactt ccccgtgccc cccgggtcat ccggccggaa
      361 gaggaatgct gtccggcctg tggtggtgaa ctcagtccac tgggatgtga tgtgtcagag
      421 cagctggagc ttatcagcag cgcctttaag gttatcgaaa aacaacgccc taaactggcc
      481 tgtcgccggt gcgaccatat cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt
      541 tatgccggag cggggcttct ggcccatgtt gttaccggaa agtatgcaga ccatctgcca
      601 ttataccgcc aaagtgatct actattccac acagctattt agctctcttt ttgttgagct
      661 cccatgattc tttcatttat tgttactaat tattttatgt tatcatgatg ttagaacacg
      721 ctctgctctg tgtctctatt ttatctatca tattctgagg attaactaca taacaaggcg
      781 caagatatat atccaaaaac aaataatacc gttgcaataa ttcctttcag taaaggaggt
      841 gtgattaatt tcaggggatt tcagagtgaa aattgtacaa catattgaaa tatgttcatt
      901 tcgttataat acacacaaga aaatattaag ttagataata tattctaaat aaccatagat
      961 aaaaatgcac ataatgggga atattttatg aaaatacctg aagcagtaaa tcatattaat
     1021 gttcagaata atattgatct t
//
LOCUS       DAJVOY010000357         1016 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVOY010000357 DAJVOY010000000
VERSION     DAJVOY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.361"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..781)
                     /locus_tag="OQL90_004227"
     CDS             complement(<1..781)
                     /locus_tag="OQL90_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1370766.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTAFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKN"
     gene            789..>1016
                     /gene="tnpA"
                     /locus_tag="OQL90_004228"
     CDS             789..>1016
                     /gene="tnpA"
                     /locus_tag="OQL90_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1370767.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINY"
BASE COUNT          234 a          241 c          243 g          298 t
ORIGIN      
        1 ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc cctgcggata tttgcgatac
       61 aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca gttgttgctg aatttgacct
      121 tgcggcttaa ctgtcgctga agtctcgcca gcttcttctg gtttttctga aaactgttta
      181 caggctcaaa ggctgtgcca tctgacagcg tggcgagttt agccacgcca gcatccagcc
      241 cgaccattga tgctgaaggg tgaaccggag ttgatacttc actttctgtc tgaatactga
      301 tgtaccactt accgcaggac tggctgacag tgacattttt cacaacaccc gtgacctgac
      361 ggctgttccg gtagcgcatc cagcccagtt tcggcagaaa aatacggctg ttttcctgat
      421 cgagcttaac gccctgcggg tagcggaata catcattctg tccccgcttt ttgaatcggg
      481 gaaaagcagc ccgattctgg aagaagtttt tgtaagcccg ctcaaggtct ttcagtgact
      541 gttgcaatgg ctgtgacggg gcatctttaa gccattgcgt ttcagtggca tttttccact
      601 caaccagcca ggaagccatt ttcccgtaag ggatgtattt attaccgacc tcatgattct
      661 cattctgacg tgccagagca cgattgaaaa cgaaacgaca tgcgcctgcg aagcgcctca
      721 tttcacgctc ctgttgacca ccgggtctta actggaattt aaatgcttgt agtcgtttca
      781 caggccaaat gatactttgg tctgtgaaaa aagaaaccga tatccggcgt ggaaggcatt
      841 gtgttttcct gatgcatgtt cacctggtct ttgtcaccaa ataccgacgt cagatttttg
      901 accatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct gattttgaag
      961 ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tttgttaatt aactat
//
LOCUS       DAJVOY010000358         1013 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVOY010000358 DAJVOY010000000
VERSION     DAJVOY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.362"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            109..>1013
                     /locus_tag="OQL90_004229"
     CDS             109..>1013
                     /locus_tag="OQL90_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCS1370768.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLKSFLLVHGVHY
                     VGRADWGPAHRRWLSKYSFESPWRQLAFDEHRRTIEDRQAQCERLESALKEAVTEWRL
                     YPVVEALQAMRGIQFITAVGLISELGDLTRFEHPRQLMSWFGITPSEYSSGGSRHQGS
                     ITKAGNSYARKLLVEAAWSYRHPAR"
BASE COUNT          248 a          241 c          277 g          247 t
ORIGIN      
        1 gagacagatg ctggccaacg tgggatattt aacgcgggtt aacatcgttg tcagagccgc
       61 atgggtgagc tgttgctaca ccccggtatt tccaccacgg aggccagcat gaacaacaat
      121 aacacattgt acgtcgggtt agatgttcac aaagaatcga ttactgtcgc ttatgctatc
      181 aattcagaac ctgttgaact gatgggtaaa attggcacat cacctactga tattcagaat
      241 ctttgtaaac gtctcaggtc aaagtcatcg caggtcagta tcgtttatga agcggggccc
      301 tgtggttatg gactttatcg tcggctggtg aaatccggtt ttgactgcat ggtctgcgcc
      361 ccctcactta tcccgaaaaa accgggggag cgggttaaaa ccgatcgccg tgatgccatc
      421 agacttgtgc gttcactgcg tgcaggagat ctttctgctg tctacgtacc cggcattgaa
      481 gatgaggcat tccgggattt ggcccgggca tgggcatctg cccgcgatga tttacggcat
      541 gcaaggcaac gtctgaaatc gtttcttctg gttcatgggg tccattatgt cgggcgagca
      601 gattggggcc ctgcacaccg acgctggctc agcaaatact cattcgaaag tccctggcga
      661 caattagctt ttgacgaaca ccgtcgtaca atagaagacc ggcaggcaca atgtgaacgg
      721 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatccggt agtagaggcg
      781 ctgcaagcca tgcgagggat tcagtttatt actgcggttg gattaatttc tgaactgggt
      841 gatctgaccc gttttgaaca tccacgacaa ctgatgtcat ggtttggtat cacgccatca
      901 gaatattcat cgggtggaag cagacatcag ggcagcatta ccaaagccgg aaacagctat
      961 gccagaaagc tattggtcga ggcagcgtgg agttaccgtc atccggcacg cat
//
LOCUS       DAJVOY010000359         1013 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVOY010000359 DAJVOY010000000
VERSION     DAJVOY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.363"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..441
                     /locus_tag="OQL90_004230"
     CDS             <1..441
                     /locus_tag="OQL90_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1370769.1"
                     /translation="GVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHAHMKAGSLTGLSI
                     GYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNALARGEIPEQKKIE
                     RVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVSSALNALKNLNF"
     gene            456..>1013
                     /locus_tag="OQL90_004231"
     CDS             456..>1013
                     /locus_tag="OQL90_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCS1370770.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGRGDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEASVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFM"
BASE COUNT          271 a          245 c          307 g          190 t
ORIGIN      
        1 ggtgtttaca ccgaaatgaa ggaagacgat gtcgggcttt acgtcagggg acggttgctt
       61 attgatgatg atcccctcgc aaaacgcgca catgcacaca tgaaggccgg ttcgttaacc
      121 ggcctttcta ttgggtacgt cctgaaagac tgggaatacg accggacgaa agaagccttt
      181 ctgctgaaag aaatcgacct ctgggaagtc agtctggtga cgttcccgtc taacgacgag
      241 gcgcggatca gcgacgtcaa gaacgcgctg gcccgcgggg aaatccccga acagaaaaaa
      301 atcgaaagag tcctgcgtga tgtcggactc tcccgtaccc aggccaaagc attcatggcc
      361 gggggctatg gcgcactgtc cctgcgcgac gctgaggatg tgagctctgc actgaatgca
      421 ctgaaaaatc tgaacttcta atcaggagaa atacgatggc ggtagatatt aaagatgtcg
      481 aacaggtcgc gcaggagctg cagcagaagt ttgacgactt caaagcaaag aacgacaagc
      541 gcgtggatgc gattgagcag gaaaaaggca agcttgccgg gcaggtggaa accctgaacg
      601 ggaaactcag cgagctggaa aacctcaaaa gcgatcttga aaaagagctg cttgagctga
      661 aacgtccggc aggtggtgcg caaaataaac tggccaccga gcataaagaa gcgtttgtgg
      721 gcttcctgcg taaaggccgt ggagatggtc tgcgcgatct ggagcgcaag gcattacagg
      781 tgggcaccga tgaagacggc ggctatgccg tgccggaagc actggatcgc aacattctca
      841 ccctgctgaa agatgaagtg gtgatgcgcc aggaagcctc ggtgatcacc gttggtggtt
      901 ccgactacaa aaaactggtg aatctgggcg gcacggcttc cggatgggtt ggcgagactg
      961 acgcgcgctc ccagactgcc acctcaaaac tgggcctgat tgaacctttc atg
//
LOCUS       DAJVOY010000360         1010 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVOY010000360 DAJVOY010000000
VERSION     DAJVOY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.364"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..772)
                     /locus_tag="OQL90_004232"
     CDS             complement(<1..772)
                     /locus_tag="OQL90_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1370771.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH"
BASE COUNT          283 a          146 c          238 g          343 t
ORIGIN      
        1 catgggatga caatgggtta tcactaatat ctattgaaca ttcattcctc agattaagaa
       61 tgctttccgg gatatgactt atctgattta tgtcaaaata accgttcctt aaattttccg
      121 gcaggtatgg aatatcggtt aacatattgt cgattgccca tatggagtga atgttgtgag
      181 gtagaggggg gagttcacta agaagattcc ctattgcgct aatctcttgt aaagaaaatg
      241 gtaaaggtgg taaaactgcc agcccattac atgatacatc taatgtttcc agtagttctg
      301 gtaaaacagg aagagaacat aattgattat ttgagacatg aagctccttc aaagatatgg
      361 ggagtgtggg tagtgtgatt agttgattgt gggacgcatt caataattta agtccttgag
      421 gcaaagcagg cagttcaata agtctgttat agctgacatt aagctgtgta agggacgcag
      481 gcaatgggga gattaagctt aaattatttt tacttatatt aattgattta attcccgggg
      541 ggatttcagg taatgttgtc aggcctaatt cagacaagtc taggttcgtc tcttggtttt
      601 gtagacatga tactagtcgc tgaaaagcga tgtctcgttg ttcttcttgt atgcggttat
      661 ttttccattc agtccaatgg gttagataat tttcatatgc gcggctagtg tcgattgaat
      721 aagtggagaa cgaatttgaa attaatctgt gattgttatt tgtcgggagc ataaatatca
      781 gggcgtattc tttatgttga gaggtgttgg attctttttt atctgctttg caatgttatt
      841 ggttcccttc agcagtagac aggaacttct ggaggaggcg gatatatagt ttgtgtaagc
      901 atattaactc catatgttat atattgaaga acttctgctt tacactatcc taaactggat
      961 cggtttaatg taaaaaaacg atactttttg ggggaggggg caatttttcc
//
LOCUS       DAJVOY010000361         1010 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVOY010000361 DAJVOY010000000
VERSION     DAJVOY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.365"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>1010
                     /locus_tag="OQL90_004233"
     rRNA            <1..>1010
                     /locus_tag="OQL90_004233"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          256 a          236 c          321 g          197 t
ORIGIN      
        1 cgatccctag ctggtctgag aggatgacca gccacactgg aactgagaca cggtccagac
       61 tcctacggga ggcagcagtg gggaatattg cacaatgggc gcaagcctga tgcagccatg
      121 ccgcgtgtat gaagaaggcc ttcgggttgt aaagtacttt cagcggggag gaagggagta
      181 aagttaatac ctttgctcat tgacgttacc cgcagaagaa gcaccggcta actccgtgcc
      241 agcagccgcg gtaatacgga gggtgcaagc gttaatcgga attactgggc gtaaagcgca
      301 cgcaggcggt ttgttaagtc agatgtgaaa tccccgggct caacctggga actgcatctg
      361 atactggcaa gcttgagtct cgtagagggg ggtagaattc caggtgtagc ggtgaaatgc
      421 gtagagatct ggaggaatac cggtggcgaa ggcggccccc tggacgaaga ctgacgctca
      481 ggtgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga
      541 tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg agctaacgcg ttaagtcgac
      601 cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg cccgcacaag
      661 cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt cttgacatcc
      721 acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt gctgcatggc
      781 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
      841 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      901 aggtggggac gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      961 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
//
LOCUS       DAJVOY010000362          999 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVOY010000362 DAJVOY010000000
VERSION     DAJVOY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.366"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OQL90_004234"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OQL90_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCS1370772.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OQL90_004235"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OQL90_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCS1370773.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
BASE COUNT          297 a          202 c          228 g          272 t
ORIGIN      
        1 gctaaagttc tgggttaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataatcat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaac ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaattg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatg
//
LOCUS       DAJVOY010000363          992 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVOY010000363 DAJVOY010000000
VERSION     DAJVOY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.367"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            69..950
                     /locus_tag="OQL90_004236"
     CDS             69..950
                     /locus_tag="OQL90_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1370774.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT          248 a          209 c          270 g          265 t
ORIGIN      
        1 acacagaaca tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt
       61 tcggggttat ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct
      121 ttttgcgagc ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc
      181 agcttgagca ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta
      241 cccgcgaagg aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc
      301 ttcgtgaggc tgccaaaaag gggtcagatc cggacgggga actacgcgtt gtttcgggcg
      361 aaacgttgct ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa
      421 aagtgcgttt atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg
      481 atgaggccga tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag
      541 agttgggtga acaatcgctg gtgctggtgg cttcaccgca aattgcagat gtcgatttta
      601 ctgagccggg aagacataac gcctgtagct ttattatcaa cgaaccgcaa tgtgtcttcc
      661 ggcagatatt tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc
      721 tgttaagcat tgaaagtatc aaacgttgtg tcgcggcgaa tatcggtgtc agttacttgc
      781 cccgctttgc ggtggaaaaa gagcttgaga gcggagaatt aattgaattg ccttttggcg
      841 aacagtcgca gaccattacg gctatgtgcg cacaccatgc tggaaaagcg gttagcccgg
      901 cgatgcacac ttttattcag tgtattgaag agtgtttttt gccgggataa aaataatgcc
      961 gggcttaaac ccggcaccca acttgtcgac ga
//
LOCUS       DAJVOY010000364          971 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVOY010000364 DAJVOY010000000
VERSION     DAJVOY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.368"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..709)
                     /locus_tag="OQL90_004237"
                     /pseudo
     CDS             complement(1..709)
                     /locus_tag="OQL90_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          232 a          232 c          217 g          290 t
ORIGIN      
        1 aatagcagtc tgtgaagaaa ttgaacccat caagcgccat gatctcttcg taaagctctt
       61 tactgatagg gatgcttctg tttttcttcc ccttcgttct gacaaaggta attcggtatt
      121 tggtcacctg tgagcgggta agatttacgg cttcacgcta gcgtgcgcct gtgcttaagc
      181 atatcttaac taccagtgcc agaattgggt cctgacgttt gcaatcagcc agcaattcaa
      241 caatctgctc atgggtaagc catgccatct ctttttctgc gatggtgaat tttcgcatgt
      301 tctccagtgg gttcggatac gaccattcgc ccagacggga tagttcgcta aaaacactac
      361 ttagatagct ttgctccagg ttaatggtga ccgggcttgc tcctttcttc catttctcgc
      421 tgaagtagat cccgcctgtc aggcgtttat ctcgatagtg ggcaaacatt ttagaggtga
      481 gatcggttgc aagaggattg cccagagcgt caaccatcaa cagcaatttg tcatagacat
      541 gctgcccagc tgtcagagat ttaccatgta gtttgaacca tagctcaacc acgtctttca
      601 gtgttcgacg atccactgat tcacccagcc agggctttgc ttcggtttct tccatcgtgt
      661 ggcgctcaaa agccagtgct tcgcctttgg tggcgaattg tttacgcaca cgacgcccac
      721 ttcgtccggc ggggtaacat tcgcaaagcc atttcccagt ggtgagtttt cgtactgcca
      781 taaaaaatgc cctccagtag agagtatttt tactgtatgt ataaccagtg tcaatgtatg
      841 aaatcctacg accatacatc tcactgaagc cataatgaag ttggctattc tttttgctat
      901 gtgagcatgt aacttttgcg gttaacctgc ggctcatttt tattttagac gcagatataa
      961 aagcaaaagg g
//
LOCUS       DAJVOY010000365          958 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVOY010000365 DAJVOY010000000
VERSION     DAJVOY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.369"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(268..363)
                     /locus_tag="OQL90_004238"
                     /pseudo
     CDS             complement(268..363)
                     /locus_tag="OQL90_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(363..446)
                     /locus_tag="OQL90_004239"
                     /pseudo
     CDS             complement(363..446)
                     /locus_tag="OQL90_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            complement(476..805)
                     /locus_tag="OQL90_004240"
                     /pseudo
     CDS             complement(476..805)
                     /locus_tag="OQL90_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          256 a          225 c          235 g          242 t
ORIGIN      
        1 cggatggtat acctccgggc catcgcggga ctgaatgtgg agggctgaag aaaaacgcgc
       61 gggatatcag tctgctgagg tatatgccgg cctgacacag gctaagtgtg cccgggaatc
      121 ggtgcaagta taaaaaacag cctgttgcgg tataagaatc gaccatgcag agagagaaaa
      181 caccggagtg gagggaaaag cagaaaagct cacgtggcat aaggcgtgga cagcggtatc
      241 gtctggtttt tcaatttcct atacgtgtac cattctggcg gcagcaggtt tactggccag
      301 caccagaaaa tcatataacc gcgccaaatc agaaagcgcc ttactggtcc acttctgttc
      361 catcagcgtg gcaccggtaa cggattgtca gtactgaggc tgtccgccca ggcctgtaca
      421 gcctgatggt cgataatctg tccggatgga ccagcgggag catttgaaca gaaacttacg
      481 gttttgctct ttcggtaggc tcagtaccgt aaggttctcc atcaacaaaa taagtaccat
      541 taggtaatac gtgtttatag ggcagtaatg atatatgggc taatacgtca tcatcaataa
      601 cctcaccatt ttcacgtaac ccttcaacaa tattgcttat tcttgatgta ttccagtaca
      661 ggattgcatt cgataccaga ctcagggcgc tggccttatt catgatttct tcgtaatcac
      721 cggtcgtgaa ttcaccctga tttgcaaaaa agatccatcg gggtaattta tgtcgatatt
      781 cgcctttatt gagctgaagc tcgagtaggc agcctgacgg ctgcggcttg tcatggtctg
      841 caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata tggcccgttc
      901 agctaaaccc cgtaaacgta aacccgcctc tcaaagaagc aaacttcccc gctatgtc
//
LOCUS       DAJVOY010000366          952 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVOY010000366 DAJVOY010000000
VERSION     DAJVOY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.370"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..195
                     /locus_tag="OQL90_004241"
     CDS             <1..195
                     /locus_tag="OQL90_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1370775.1"
                     /translation="RKKLSRAKLKKPSAVDPDLIWSLPDGEIRRYDSRLNIICRECRK
                     SEVMQRILSFYQGNFQEVMA"
     gene            192..497
                     /locus_tag="OQL90_004242"
     CDS             192..497
                     /locus_tag="OQL90_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCS1370776.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            484..939
                     /locus_tag="OQL90_004243"
     CDS             484..939
                     /locus_tag="OQL90_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCS1370777.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSAPDVLALLKELEVKDKRIAELTDALTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
BASE COUNT          271 a          188 c          264 g          229 t
ORIGIN      
        1 cgtaaaaagc tctcaagggc aaagctgaag aaaccatctg cggttgatcc ggaccttatc
       61 tggtcattac ctgacggaga aatacgtcgc tacgacagtc gcctaaacat aatctgtcgc
      121 gagtgccgga agagcgaagt tatgcagcgc atattgtcgt tttatcaggg taattttcag
      181 gaggtgatgg cgtgagggtg agagtctata tcgccggtcc aatgaccggg tataaaaatt
      241 tcaaccgtga ggcgtttcac aatgcggaag aggaactgaa acgggaaggg cataccgtct
      301 taaacccggc agtacttccg gacgggctga cacagccgca gtacatggat atctgcatgg
      361 cgatgattcg ttgtgtggat gcgatttaca tgctgaaagg ctggcagcgg tcagcaggcg
      421 ctaaggcaga actggcgctg gcggagaaac tggggcatgc ggtgattttt caggagacag
      481 ccagtgtgca agactaatta ccaggcatta cgtgaacgtt attcaccaat tcaggtgccg
      541 gaatgtcctg tttgtggcga tgaaatgtcg atacagcgca tattttccag aacgcatatt
      601 gtgtatgcct gcacaggtga gggggatgat ggatatttta aaactggtcg aacttttgcg
      661 gatgagcatt acctgaaatc gcgcgtaacc gtcgttgatg ttagcgctcc tgacgtactg
      721 gctttactga aagagctgga agttaaagac aaacgcattg cagaattgac agatgcgctt
      781 acacaaatga ttaatgcgca caaaaccaca attcgttttg gtcatgaacg cataactgaa
      841 tgtggtggtg attgcgactc gccggaaaag atgatttcag aaaatccgga tatcagaatg
      901 gcagaggctg ttttgagagc aggaataaaa actgaataat taaatttagc ac
//
LOCUS       DAJVOY010000367          947 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVOY010000367 DAJVOY010000000
VERSION     DAJVOY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.371"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..181
                     /gene="tnpB"
                     /locus_tag="OQL90_004244"
     CDS             <1..181
                     /gene="tnpB"
                     /locus_tag="OQL90_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1370778.1"
                     /translation="LWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGI
                     AWQHPKRTERPGIRI"
     gene            201..>947
                     /locus_tag="OQL90_004245"
     CDS             201..>947
                     /locus_tag="OQL90_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCS1370779.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKL"
BASE COUNT          262 a          249 c          251 g          185 t
ORIGIN      
        1 actgtgggcc gatcgtgacg ggttatgcct gttcgccaaa cgcctggaac ggggccgctt
       61 cgtctggccg gtgacccggg aagggaaagt gcacctgacg ccagctcagt tatccatgct
      121 actggagggg atcgcgtggc aacatcccaa acggacagaa cggcctggca tccggatata
      181 acccgtgata aaacaaggga atgaacaatg aactccccga tgatattgag ctgcttaaag
      241 ccatgttgcg taagcaacag agtcggcttc gacagtatgc ctgtcaggtc gcgggctatg
      301 agcaggaaat tgaacggctg aaagcgcaac tcgacaggtt gcgtcgtatg ctgttcggcc
      361 agagttcaga gaaaaagcgt cataagcttg aaaatcagat ccgacaggca gaaaaacgac
      421 tgtcggaact ggaaaaccgg ctgaacacag ccagaaatct tctggaagat gcatcgtcag
      481 tcacagattc acctgacacc agtcccccgt cagaaaaccc gatcgccagt aagcctgaat
      541 ccccgggacg aaaatcttca cgaaaaccgc tgccggcaga acttccccgg gagacacatc
      601 gccttctgcc tgctgaaacc agttgcccgg cctgtggagg tgttctgaaa gaaatggggg
      661 aaacaatctc agagcaactg gatatcatta ataccgcctt taaagttatc gaaaccatac
      721 gtcccaaact ggcctgtagc cggtgtgatg tcatcgttca ggcaccactt ccccctaaac
      781 cgatcgaacg cggttatgcc agtgcagggt tacttgcacg gatcctggtc agcaaatata
      841 tggaacatat ccctttatat cgccagtcag aaatatacgc gcgacagggc gtggagctga
      901 gccgtaatac catggtgcgc tgggtatcag aaatggcaga caaactc
//
LOCUS       DAJVOY010000368          933 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVOY010000368 DAJVOY010000000
VERSION     DAJVOY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.372"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..557
                     /gene="envY"
                     /locus_tag="OQL90_004246"
                     /pseudo
     CDS             1..557
                     /gene="envY"
                     /locus_tag="OQL90_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
     gene            complement(603..679)
                     /locus_tag="OQL90_004247"
     tRNA            complement(603..679)
                     /locus_tag="OQL90_004247"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(643..645),aa:Arg,seq:tct)
BASE COUNT          260 a          226 c          214 g          233 t
ORIGIN      
        1 ttttaaataa gaacttaacc tgtgtaaagc cctagtcgcg gctggcaacc ccggttatcg
       61 cttgtcatag ccgtacaccg gaagtgttcc ggctagccgc caaccacagc aagcagcaac
      121 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
      181 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
      241 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
      301 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
      361 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
      421 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
      481 tctctgtttt taaggcgttt tacggcctga caccgttgaa ttatctcgcc aaacagcgac
      541 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
      601 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
      661 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
      721 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
      781 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
      841 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
      901 gctcctatga gactggtaac tatgaaacca acg
//
LOCUS       DAJVOY010000369          927 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVOY010000369 DAJVOY010000000
VERSION     DAJVOY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.373"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..706)
                     /locus_tag="OQL90_004248"
     CDS             complement(<1..706)
                     /locus_tag="OQL90_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1370780.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESFECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFT"
BASE COUNT          242 a          225 c          221 g          239 t
ORIGIN      
        1 cggtgaattc gacattgccg aatttgtcga agtgaacaac cagagtgcga ccatcacccg
       61 gaatcatctt gtcagcaggt ggggtgttat tctggcgcag ttcggcctcc atgcggtcga
      121 actcagcaat gtaggcttcc ttgaatgtgg cggctttttt gcccgtgaag cccatcacca
      181 ggaaaacgaa gccgtttttg gtgatttggt aggcgttata agaattgccg cggtgttcga
      241 atttaacccg cgaaaagttg ctggttaaaa atagttcaga acattcgaag gattctattt
      301 tttgaacaac atggtgatgc tgcttgccaa aaaactcagc aatcgaaata gaagtagtga
      361 cggcgcgacc attttcgatg gttacgtcag ggtgagaaag ggtagtagcc ataatgacat
      421 cctcaagtga taagttaatt aactcaccag cagaggtgct aatctcatgg gtggtgagac
      481 gcacagggtt agcactaccg gtcactcgag aacccggcca gccttgcggc tgccccgcac
      541 gcctcaccat aatttgaatg tggctgtgca ttacgcataa aaaaaccgct tcagcgcggt
      601 tatgcgctcg agtaactttc ggggtgctaa tcccggcacc cgttttatga ggtgcaggtg
      661 cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc tctttccttt
      721 catcaccgaa gtgaattttg tgatgcggtg cctagtgcct ccaggtgacg ttaaccagtt
      781 aacaattaca gtcggctttc ccacccaaac caataaggac taacatgact tttaactgtg
      841 ccgcgtgcgc ttagccgcat tcaccgcatc acaaaattca ctttaaaaag ggcggacatc
      901 agccagcaat taaaccgatg ccgccaa
//
LOCUS       DAJVOY010000370          926 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVOY010000370 DAJVOY010000000
VERSION     DAJVOY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.374"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..151
                     /locus_tag="OQL90_004249"
     CDS             <1..151
                     /locus_tag="OQL90_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIP domain-containing protein"
                     /protein_id="HCS1370781.1"
                     /translation="RLAQMQIPADDYFIWITGEGKVVKNLSRRFEAEQYDPQRVRAAA
                     YWHAK"
     gene            complement(205..280)
                     /locus_tag="OQL90_004250"
     tRNA            complement(205..280)
                     /locus_tag="OQL90_004250"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Ile,seq:cat)
     gene            405..911
                     /gene="mug"
                     /locus_tag="OQL90_004251"
     CDS             405..911
                     /gene="mug"
                     /locus_tag="OQL90_004251"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCS1370782.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          236 a          213 c          256 g          221 t
ORIGIN      
        1 gcgtctggcg cagatgcaaa tccctgccga tgattacttc atctggataa ccggcgaagg
       61 taaagtcgtt aagaatttaa gccgccgctt tgaagcggaa cagtatgacc cacagcgggt
      121 acgtgcagcg gcttactggc acgcaaagta actgagatat aaaaagaaaa aggctgacga
      181 tttctcgtca gcctttgcta tatctggtgg cccctgctgg acttgaacca gcgaccaagc
      241 gattatgagt cgcctgctct aaccactgag ctaaggggcc atggtagcgg attataaagt
      301 aactccgtgt cgcaatccag ccattaccgt gcgcctgctg tttttataaa caatgtattt
      361 tcaatccgtt atacttttcc tggtgatgtt agaaaggagt aaatatggtt gaggatattt
      421 tggctccagg gttacgggtc gtgttttgcg gtatcaaccc tgggctttca tccgccggga
      481 ctggttttcc ctttgctcat ccagcaaatc gcttctggaa ggtgatatat caggccgggt
      541 ttaccgaccg tcagttgaag ccgcaggagg cacagcatct gctggattat cgttgtggcg
      601 tcaccaaact ggtagaccgt ccaacggtgc aagccaatga agtttcaaag caggagctgc
      661 acgcaggtgg gcgtaagctg attgaaaaaa ttgaagatta tcagccgcag gcgttggcga
      721 ttctgggcaa acaagcatat gaacagggat tcagccagcg cggtgcacag tgggggaaac
      781 aaacgctcac cattggttcg acgcagattt gggtgctgcc aaatcccagc ggtttaagtc
      841 gcgtttcact agagaaactg gttgaagcgt atcgcgagct ggaccaggcg ctggtagtgc
      901 gtgggcgata aaaaacgcca ccgaaa
//
LOCUS       DAJVOY010000371          914 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVOY010000371 DAJVOY010000000
VERSION     DAJVOY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.375"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>914
                     /gene="tuf"
                     /locus_tag="OQL90_004252"
     CDS             <1..>914
                     /gene="tuf"
                     /locus_tag="OQL90_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1370783.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          212 a          230 c          264 g          208 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccg
//
LOCUS       DAJVOY010000372          911 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVOY010000372 DAJVOY010000000
VERSION     DAJVOY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.376"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..852
                     /locus_tag="OQL90_004253"
     CDS             <1..852
                     /locus_tag="OQL90_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCS1370784.1"
                     /translation="PLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRD
                     DRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC
                     LAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQ
                     SLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAV
                     GRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLP
                     WNFSSVK"
BASE COUNT          239 a          212 c          277 g          183 t
ORIGIN      
        1 cctctgtata tagcgctgaa tgactatgtt ctggaggcag gaaaggtgca cgcagatgac
       61 actccggtga aagtactggc cccggggaac ggaaagacga aaacgggtcg tctgtgggta
      121 tacgtcaggg atgatcgtaa tgcgggttca tccctgccgg cagccgtctg gttcgcgtat
      181 tcggcagatc gcaaaggaga acatccgcag ctccacctgg caaagtatca gggcgtactg
      241 caggctgatg cctatgcagg ttataacgta ctgtacgaaa cgggccgggt gaaggaagcc
      301 gggtgcctgg cccacgcccg ccgaaaaatc catgacgagg atgtgcgccg tccgacagaa
      361 atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc ggagatacgt
      421 ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca gttgatgcag
      481 tcattgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc ggagatggcg
      541 aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg tcgtgacggc
      601 tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc ggttggaaga
      661 aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat catctacagt
      721 ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg cgaagtgatc
      781 gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg gaacttctcg
      841 tctgtaaaat aatccttacg ctacgtactt ctcggggcgc ttacaccgtt actgttcatt
      901 gtccgcgctg t
//
LOCUS       DAJVOY010000373          858 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVOY010000373 DAJVOY010000000
VERSION     DAJVOY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.377"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(334..636)
                     /gene="hicB"
                     /locus_tag="OQL90_004254"
                     /pseudo
     CDS             complement(334..636)
                     /gene="hicB"
                     /locus_tag="OQL90_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            complement(682..835)
                     /locus_tag="OQL90_004255"
                     /pseudo
     CDS             complement(682..835)
                     /locus_tag="OQL90_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
BASE COUNT          250 a          182 c          176 g          250 t
ORIGIN      
        1 aagttattga accaaaatat gattctgaat ataaacacac tcaatttgca tgtgacatgg
       61 tagacgcaga gtaagaggtg aaatgatgca attcaatagc atgaagatta ctcaaaaatg
      121 gtgggatgcg atgaaagcta accaccaaaa cgatattaat taagtaacat tttagatata
      181 cagcacccag ctaacagctc accttttatg gatttcatta taaccccttt gatctggaaa
      241 ctatgttaat tagttgattt ctaagccttg tccttgtgat ggggcttttt attgcaaaat
      301 ttttagttat tttgggttga ctacattcgg gttgccaatt cgcctggcta actcttgctg
      361 agtgatttct gactgtaaaa aagcatttaa cagcaatact ttagaggcga cgctcaaagg
      421 tacttcaata aagtgatcat gactatttaa tggcgaaggt aacgggataa gctcgttatc
      481 ttcaaaataa aaatcaaatg cggtcagtaa agcatctttt gccgcttcca tcgcttcagc
      541 gacagtttcg ccctgcgtca acgcttcagg gatatccaca aaagaaacca tataaccgcc
      601 ttccggcgcg ggtgtaagag tgacgggata acgcataatt tcgactgtct ctcgcatgtc
      661 attaaccttt ttaattggcg attaactcaa accgagttgt ttcaggattg ctttacgcag
      721 gagtgacgcg gcatgacact gcgcctccca tgaaacctga gtttcaaatg gttgcggcat
      781 tcgctacatc gacgccctga gattcgagcc aacgtctgaa ctcgctttgt ttcacatcgc
      841 ctccctgccg ctgagtat
//
LOCUS       DAJVOY010000374          856 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVOY010000374 DAJVOY010000000
VERSION     DAJVOY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.378"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..778
                     /locus_tag="OQL90_004256"
     CDS             <1..778
                     /locus_tag="OQL90_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1370785.1"
                     /translation="NILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADEQ
                     AAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPEF
                     VARHIAEWQHQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLDALFIT
                     LEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPAM
                     QCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
BASE COUNT          204 a          198 c          272 g          182 t
ORIGIN      
        1 gaacattctg ttagcgttta aagccgaacc ggatgccgga atgctggcgg aaaaagagtg
       61 gcaggcggcg gctcagggta aaagcggacc ggatatttcg ctactgcgaa gtttactcgg
      121 tgctgatgaa caggccgctg ccgcgctgct gttggcgcag agaaaaaacg gtacgccgat
      181 gtctttaacc gcgttgagca tgggggatga acgggcattg cactggctgc gctatctcat
      241 ggctcttggg tttgaggaag ctgtattgct ggagacggcg gcagatctgc gctttgcgcc
      301 ggaatttgtc gctcgccata ttgccgaatg gcagcatcag aatccgctgg atctgataat
      361 taccggcagc caaagcagcg aagggcagaa tgggcaaacg ccatttttgc tggcggaaat
      421 gctgggctgg acctgcttca cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac
      481 cctcgaacag cgtactgaac atgggttgcg ttgttgccgg gttcgcttgc ctgcggtgat
      541 tgccgtgcgt cagtgcggtg aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc
      601 tgcggggaaa gcagaaatta tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg
      661 tctgcaactg gcaagagcag agcaacgacg cggagcgacg ctgattgatg ggcaaacggt
      721 agcagaaaaa gctcaaaagc tatggcagga ctatttacgc caaaggatgc agccatgaat
      781 atcgcaattg tgacaataaa tcaggagaat gccgcaatag caagctggct ggcggcgcag
      841 gatttcagtg gttgta
//
LOCUS       DAJVOY010000375          849 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVOY010000375 DAJVOY010000000
VERSION     DAJVOY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.379"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..>849)
                     /locus_tag="OQL90_004257"
     CDS             complement(<1..>849)
                     /locus_tag="OQL90_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1370786.1"
                     /translation="DELKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILL
                     CCLFGVLACTLFAWNRNYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFS
                     TFLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPV
                     VTQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIG
                     IAAGLEIPMMLIAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAI
                     FLGILC"
BASE COUNT          262 a          200 c          194 g          193 t
ORIGIN      
        1 cacagagaat accaaggaag atggcattta gaatttgcaa ttccagctca accgccggag
       61 tcgtcgccat gagtacgctg gcgtaaaaac acataccact cacgatagca atgagcatta
      121 atagtcgctt gccaatacgt ttcatgtaat agcctgcgat taacatcatc ggaatttcca
      181 gaccggcagc gataccaatc atttctccag ccagtttatc ggttagatgc agttcatcaa
      241 taataaatag cggcatattt atcatgtaga gattattcgc cgcccacatc attgaacaga
      301 ccacaaaaag tagccgcgta tcccgcttcc tatgggtgga gggtaaaatt tctacgggtt
      361 gggtaacgac aggaatattt ctttgtattg atggcaaaaa caaccagact atcagcccgc
      421 aaacaacaaa tgcgatggca gcggtgagat acatcacttt aaaactaaac cccattgcca
      481 gttcataagc gagcggtggc ccgataaccc aggcaagcga gatctgagca cgtaaaaatg
      541 tactgaacat gaccgtctca cggcctgttc tgtcggcgtg ttcacgggcg agggcgaaca
      601 tttgcgggtt tgcggtagaa gcaaaactac tcagaagtac gcccgtagag aggagaatga
      661 agtagttgcg attccacgca aaaagcgtgc aggccagcac tccaaataag caacatagca
      721 gaatcagtaa tttacggtcg ccttgtttat cggagtgcct tgccagaaat tgactgacca
      781 gaattcccat aatagcgcta ccggtgaaga aaaaacctac cattatagga cgggctttca
      841 gttcatctg
//
LOCUS       DAJVOY010000376          847 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVOY010000376 DAJVOY010000000
VERSION     DAJVOY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.380"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(29..217)
                     /locus_tag="OQL90_004258"
     CDS             complement(29..217)
                     /locus_tag="OQL90_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1370787.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            478..813
                     /gene="iraM"
                     /locus_tag="OQL90_004259"
     CDS             478..813
                     /gene="iraM"
                     /locus_tag="OQL90_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1370788.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          305 a          190 c          130 g          222 t
ORIGIN      
        1 aatgccgggc cagtacgatg acgtagtatc actccggctc agttgcacca ctgaccacca
       61 catcaccttc tgctgcaatc gcctgcatca gggtataagg ggttatggcc accggactac
      121 caaacggctg ccagccctct ttcagtttat gtgtcagctt ttccgcaaga tctgacggcg
      181 gcgccgccct gacaacatca tagtgtttaa atgccatggt tctttccacc atctgaaaaa
      241 taattcttta aaatacctga catgtaatac agaaaaaaca caaaaccata ccttaaataa
      301 aaacctgatt atcaagcaga tatgcatgga taaactacaa gacgagatat aaaccaccct
      361 gcatttaaat aaacaataaa caacatcaga aaaataattc tgctctatgg tttacattca
      421 aaaatatcat ttatactttt cagaacatca ccagcaaggc ataaacaagg aaactaaatg
      481 aagtggattg tgattgatac agttatccag ccatcatgcg gaatatcttt ttcagtcata
      541 tggagtaaaa taaaattaat aatctggtat caatcggatg ctttcttacc tcctgaaagt
      601 atatttacac tgactcacac aggcatcatg ctcaataaca aagtgctacc tgtaaccatt
      661 tacaacgtag taccattcaa taaaacattc tggaatttaa tcaaaaacag ccaggaatgc
      721 cctacaaata cagataacgt attgaatgaa tgctttaata accgttgcac tctgcaaata
      781 tgtccttatg gactaaaaca acaaagtcca taaggagttt actcacatct gacaaaatca
      841 atataaa
//
LOCUS       DAJVOY010000377          837 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVOY010000377 DAJVOY010000000
VERSION     DAJVOY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.381"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>837
                     /locus_tag="OQL90_004260"
     CDS             <1..>837
                     /locus_tag="OQL90_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1370789.1"
                     /translation="AWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAKLTI
                     GQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNRIIY
                     QENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYTTAM
                     GKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLHGGSVLTNRDYQSLAAGVGFNLNSLGS
                     LSFDVTRSDTQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYIN
                     DT"
BASE COUNT          231 a          189 c          172 g          245 t
ORIGIN      
        1 ggcctggcgc ctgcgtactg attaccagta cgatcagtca tttaacaatg gcaaaagcca
       61 ggcgaacaac ctggattttc cgcgtattta tttgtttcgc ccaatcccag caattaatgc
      121 aaaactaact ataggtcaat acgatactga atcctctatt ttcgactctt tccatttttc
      181 tggcgtttcg ttgaaaagcg atgagaatat gttgccgcct gatctacgtg gttacgcacc
      241 gcaaatcacg ggtgttgcac aaacgaatgc aaaggtcact gtctcacaga acaaccgtat
      301 tatttatcaa gaaaatgttc ctcctggccc atttgctatt actaatttat tcaatacatt
      361 acaggggcaa ctggacgtca aggttgaaga agaggacggg caagtcacgc aatggcaagt
      421 tgcatctaat agtattcctt atctgacgcg taaagggcag attcgctaca ccactgctat
      481 gggtaaaccg accagcgttg gtggtgattc cttacaacaa cccttcttct ggactggtga
      541 attctcatgg ggttggctga acaatgtatc cctgcatggt ggttcagttt taacaaaccg
      601 tgattatcaa tctctggctg ccggcgttgg ctttaacctt aactcattgg gttcattatc
      661 ttttgatgtc acacgatctg atactcagtt gcataatcag gataaagaaa cgggttatag
      721 ctaccgcgct aactattcaa aacgttttga atctaccggt agccagctca ctttcgctgg
      781 ttaccgtttc tctgataaaa actttgtgac aatgaatgaa tatatcaatg acactaa
//
LOCUS       DAJVOY010000378          831 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVOY010000378 DAJVOY010000000
VERSION     DAJVOY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.382"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(71..493)
                     /locus_tag="OQL90_004261"
     CDS             complement(71..493)
                     /locus_tag="OQL90_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370790.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVVVH"
     gene            complement(477..704)
                     /locus_tag="OQL90_004262"
     CDS             complement(477..704)
                     /locus_tag="OQL90_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1370791.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
BASE COUNT          208 a          236 c          176 g          211 t
ORIGIN      
        1 gccgaacctt cctccggata taatctgtga ttccccaatc aacagaacca gaggaggttc
       61 ggcataaaca tcaatgcaca acgacagaat cgtcggacga cccaccaccg ctgaaatgtg
      121 ctttccggta aacggcctga actgcgtcat catgcgcatc aattgccgta ctcaacgctt
      181 cctgcgccgc cagtaatgca cggcgttcca gggtatcgaa gatacagagt cggtgacgca
      241 gctcacgcgg aagaattgcc agaaccgcag ggatcagttt ctgaattttc tccctttgcg
      301 ctttcgtttc acctttcaac caacggtgat agatattctg ctgattgttc cagtccttgc
      361 ctggtaccag aggcaattcg ccgcccccct gccgcagata ttcttcagta attgcgttag
      421 cgacccacgc ctgccctttt tcggctgcca gggctaacag cactgattcg atgtgctcat
      481 gcctgatttt catgaatcac cttcccaggt cgttttgcct tacgatattc gtcataaact
      541 ttggggtcgt actgaagttc cccgccagat gcctcttgca ggcgcatcgc gcgaccttca
      601 ggaaccagtt ccccccatgc agcaatgctt gccagcctaa ctcctgcggc attggcaagc
      661 tttgttttgc tgccaaaaaa cgctatagca tcaattttca acatatcgag ctccttagat
      721 tttcctaagg aaactagatc gtagagaaac ctaagtcaag aaagattaga attccctaat
      781 atgaaaaacg aaaccttcgg tgctcgcctc ttacataggc gtaaaaaatt a
//
LOCUS       DAJVOY010000379          802 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVOY010000379 DAJVOY010000000
VERSION     DAJVOY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.383"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..347
                     /locus_tag="OQL90_004263"
     CDS             <1..347
                     /locus_tag="OQL90_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370792.1"
                     /translation="VMCLTKPAFSYYIAAYLKLFIENYYEADALVDTILNMLTPPVRN
                     GIPSTTWIAKNLDCFSRNKRVAISFTFQYLCNEYNDPNTEKALNIYWGQYLNESLLIK
                     NVQNSKNKCKSQ"
     gene            complement(408..587)
                     /locus_tag="OQL90_004264"
     CDS             complement(408..587)
                     /locus_tag="OQL90_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370793.1"
                     /translation="MVAVAAAVTDALLVFFRQLAPFVVLPALGIFIIHGTVRQAVGFI
                     VMPERDQALMSLTVI"
     gene            592..795
                     /locus_tag="OQL90_004265"
     CDS             592..795
                     /locus_tag="OQL90_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCS1370794.1"
                     /translation="MMKNQMEPEYTPLRKIHLYHCDHRGLPLALIRSDGRTGWRVEYD
                     EWGNLLSEDNPHRERSSEVHFLY"
BASE COUNT          249 a          169 c          186 g          198 t
ORIGIN      
        1 ctgtcatgtg tctcactaaa ccagcattct catattatat tgcagcctat ttaaaacttt
       61 ttattgaaaa ttattacgaa gctgatgctt tagtggatac tattttaaat atgctcactc
      121 ctcctgttag gaatggtatc cccagcacta catggatagc aaaaaatctt gattgcttta
      181 gtcgtaataa aagggtggct atttctttta cattccaata tttatgtaat gaatataatg
      241 atccaaatac tgaaaaggcg ttaaatatat attggggaca gtatctgaat gaatcattgc
      301 ttattaaaaa tgtacagaac tctaaaaata aatgtaaatc acagtaagac agaaccaagc
      361 agaaatcgtg ggcgtggttc gcaccctgac ggagggtgta gcgtgtacta gatcacagtg
      421 agcgacatca aagcttggtc acgttctggc attacgatga agccgaccgc ctgacgcacc
      481 gtaccgtgaa tgatgaagat gccgagtgct ggcagtacaa cgaacggggc tagctgacgg
      541 aaaaagacga gcagcgcatc cgtgacggcg gcagccacag ccaccattat catgatgaaa
      601 aaccagatgg agccggaata cacgccgctg cgtaaaattc acctgtacca ctgcgaccac
      661 cggggactgc cgctggcgct gatacgcagt gacggccgaa ccggatggcg tgtagagtat
      721 gacgaatggg gcaacctgct gagtgaggat aacccgcaca gggaaagatc atcagaagtt
      781 cactttttgt actaaataat tc
//
LOCUS       DAJVOY010000380          798 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVOY010000380 DAJVOY010000000
VERSION     DAJVOY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.384"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..406
                     /gene="cybB"
                     /locus_tag="OQL90_004266"
     CDS             <1..406
                     /gene="cybB"
                     /locus_tag="OQL90_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1370795.1"
                     /translation="MIHVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLG
                     HLVIYLLFIALPVIGLVMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLG
                     YFVIGLHAAAALAHHYFWKDNTLLRMMPRKRS"
     gene            651..>798
                     /locus_tag="OQL90_004267"
     CDS             651..>798
                     /locus_tag="OQL90_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370796.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSIS"
BASE COUNT          204 a          174 c          186 g          234 t
ORIGIN      
        1 catgattcat gtttcctgcg gcatctcaat tctcgtgctg atggtcgttc gtcttttgtt
       61 aaggctgaaa tacccaaccc cgccgattat acctaagcca aaaccgatga tgacgggact
      121 ggcgcatttg ggacatttgg tgatttatct cctgtttatt gcgctgccag tgattggttt
      181 ggtgatgatg tataaccggg gcaacccgtg gtttgcgttt ggtttgacga tgccttacgc
      241 ttcagaggcc aatttcgaac gggttgatag cttaaagtcg tggcatgaaa cgctggcgaa
      301 tctgggatat tttgtcatcg ggttgcacgc tgcggctgca ctggcacacc actatttctg
      361 gaaggacaac acacttctac gcatgatgcc gcgtaaacgt tcctgaagga tatttaaaga
      421 aaacgcctgt actaaaaccg acccgtggta caggcgaaga atacgggtct acatcggaag
      481 cgcctattat atttatttgt atgacaaata aaaccataat ccttgcccat acgtccatct
      541 ggcttatttt taatcaattc acccgatctt tgatctcatc aacggtatca aaataaacca
      601 gcgtataacg ttcaaatcga acataagatt cgataaacca tggaggttat atgaaaaaac
      661 tggcacttat tttgtttatg ggaacgcttg tttcctttta tgccgatgcc gggcgcaaac
      721 cctgttctgg ttcgaaaggg gggatctcac actgtacggc aggcggcaaa tttgtctgta
      781 atgatggttc tattagtg
//
LOCUS       DAJVOY010000381          793 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVOY010000381 DAJVOY010000000
VERSION     DAJVOY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.385"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(165..788)
                     /locus_tag="OQL90_004268"
     CDS             complement(165..788)
                     /locus_tag="OQL90_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1370797.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
BASE COUNT          158 a          249 c          191 g          195 t
ORIGIN      
        1 cacagaccgg actgactccg gaaaaacaga ggcccgcccc cgggcctccc cggattcatc
       61 cgtttccctg ttcagcctga cagcacgccc ccgcggccgg atgacagact ccgcttcggt
      121 aagcaaagcg gtcttctgtg attccgccag ttgcggctta ttcattactc aacgtcaaac
      181 gcccgaattg aagccaaatc atccagaccg ctcagctcct ctttcatttc acgctgacgg
      241 cgataaatct catcgttgcg atcgacctgc gcctgcacca ttgctgccgc cagttcttcc
      301 agttccggca tcgacagttt cacctgctga ttatcggcat cgctccacgc catatgtgtt
      361 tgtgctgtga cagattttgc cagcatgact accggggaca ggcggcccag tgagtcggga
      421 ccagcattcc agatacgacc gttccattca aacgtgaacg gcttcgcctc ctgttctgcg
      481 cgccatgctt caatttcctg acgtctggcc tctctggccg cttccagcat ttctggtgtc
      541 acagtgaatg gggctatctc accccatttg ccgctttgca gttcctgcca gatttgctga
      601 cccgtcggag cgacatcatc agcggtggct gtgtagggga ctgcctggtc cctgtcgtca
      661 aaaaaaacgt cacagtctac tgcgccactt tcggtataac ggggattaat gattttttta
      721 atttccacgg tgcattcctc acgatgtgcg aataaaaagc ccgggcattg cgccagagac
      781 atgtgcatcc ggc
//
LOCUS       DAJVOY010000382          791 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVOY010000382 DAJVOY010000000
VERSION     DAJVOY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.386"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(231..305)
                     /locus_tag="OQL90_004269"
     tRNA            complement(231..305)
                     /locus_tag="OQL90_004269"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(270..272),aa:Gly,seq:tcc)
     gene            complement(309..384)
                     /locus_tag="OQL90_004270"
     tRNA            complement(309..384)
                     /locus_tag="OQL90_004270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(349..351),aa:Thr,seq:tgt)
     gene            complement(387..463)
                     /locus_tag="OQL90_004271"
     tRNA            complement(387..463)
                     /locus_tag="OQL90_004271"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(427..429),aa:Arg,seq:tct)
     gene            complement(465..541)
                     /locus_tag="OQL90_004272"
     tRNA            complement(465..541)
                     /locus_tag="OQL90_004272"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(505..507),aa:Ile,seq:cat)
BASE COUNT          234 a          196 c          177 g          184 t
ORIGIN      
        1 aagaaaaaaa cgcaaaagcg ggcgaaacga tatatacagt aaggaaagca ctctatccaa
       61 caaaccaccc acagttaatc ggaataaaag cagagtgctt atgaatgatc gcacgcccga
      121 aggttagtat ttctgcacag caattttgca aaaaaaggcg atcattcata acttaaacgt
      181 ctttcagtca ctccgggatt tcccatcatc gcagactgaa agactctgac tggagcgggc
      241 agcgggaatc gaacccgcat catcagcttg gaaggctgag gtaatagcca ttatacgatg
      301 cccgcatatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
      361 ctctgcctgc tgagctaagt cggcgctggc ccaccaccga ggactcgaac ctcgcaccgt
      421 caacttagaa ggttgatgct ctatccggat gagctagtgg tggttggtgg cccttgctgg
      481 acttgaacca gcgacctggc gattatgagt cgctcgctct gaccaactga gctaaagggc
      541 cggaggcaga ataataacca tatgtcatca catctgcaaa ctcatctgac caccagcacg
      601 tttaacgtcc tgtgccgttt ttcaggtata aaaaaacccg cataaagcgg gctctttcaa
      661 atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa gaattgaagc
      721 agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata accattggtt
      781 tgtacaaaat a
//
LOCUS       DAJVOY010000383          781 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVOY010000383 DAJVOY010000000
VERSION     DAJVOY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.387"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..340
                     /gene="tnpA"
                     /locus_tag="OQL90_004273"
     CDS             <1..340
                     /gene="tnpA"
                     /locus_tag="OQL90_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1370798.1"
                     /translation="LRKLCEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGK
                     SSLMLYEQFGDLKFKYRNREFWCRGDYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIP
                     CPGSPFTDRK"
     gene            complement(458..>781)
                     /locus_tag="OQL90_004274"
     CDS             complement(458..>781)
                     /locus_tag="OQL90_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1370799.1"
                     /translation="VNRHLAEVGGITGRTIFISSMSELLKNMALNGYGIAWLPIWSIV
                     DELQTKRLICLDAAKLTVPIQAYIYRMNTRLNRTAENLWRILQEHMPDDLIQQISMEE
                     PARRN"
BASE COUNT          208 a          159 c          214 g          200 t
ORIGIN      
        1 tttgagaaag ctgtgtgagt ggaaaagcgt acggattctg gaagctgaat gctgtgcaga
       61 tcatatccat atgcttgtgg agatcccgcc caaaatgagc gtatccggct ttatgggata
      121 tctgaaaggg aaaagcagtc tgatgcttta cgagcagttt ggtgatttga aattcaaata
      181 caggaacagg gagttctggt gcagggggga ctacgtcgat acagtgggta agaacacggc
      241 gaagatacag gattacataa agcaccagct tgaagaggat aaaatgggag agcagttatc
      301 gattccctgt ccgggcagcc cgtttacgga ccgtaagtaa cgaagttgga tgcaaatgtc
      361 agatcgtgtg cgcctgttag ggcgcggctg gtaagagagc cttataggcg catttgaaaa
      421 acctccggct atgccggagg atatttattg cacgtcgtca attgcggcgt gctggctctt
      481 ccattgatat ctgctggatt aaatcgtctg gcatatgttc ctgtaaaata cgccagaggt
      541 tctcggcggt tctattcagt cgggtgttca ttcggtaaat ataagcctga atggggactg
      601 tcaatttagc agcatcaagg catatcagac gtttggtctg tagttcatcg acgatcgacc
      661 agatcggtaa ccaggcaatg ccgtatccgt tgagcgccat attctttaat aactcgctca
      721 tcgaagagat aaatatcgtg cgcccggtaa tacctccaac ctctgccaga tgacgattga
      781 c
//
LOCUS       DAJVOY010000384          775 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVOY010000384 DAJVOY010000000
VERSION     DAJVOY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.388"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            122..775
                     /locus_tag="OQL90_004275"
                     /pseudo
     CDS             122..775
                     /locus_tag="OQL90_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          299 a          136 c          124 g          216 t
ORIGIN      
        1 ggctattgtg cgctcataca ctcaaattaa agataggttc taaataaatg agcgtttttt
       61 gatagtctat ttcattaggt aatatatatt tgtaacaaat caatcaaaat ggaataaaat
      121 catgctacca tctatttcaa tcaacaatac cagcgcaact tacccagaat ccattaatga
      181 aaataacaat gatgaagtta atggattagt acaagagctc aaaaaccttt ttaatggtaa
      241 ggaataagca cctgtattaa acatctactt gagcttataa aaaacgccat acgagtaaac
      301 gacgatcctt atagatttaa tattaataat tcctcagtta cttatattga tattggctcc
      361 aatgatacag accatattac tattggtatc gacaaccaag aaccaataga attacctgcg
      421 aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt tgagaagact
      481 catgatatca ataacaagga aatgatcttc agcgcattaa aagaaatata tgatggagct
      541 cctagtttta tcttcgataa gatatcacac aaactcagac atacggtaac ggaatttgat
      601 gagagcggga aaagcgaacc aacggactta tttacctggt acggtaaaga taaaaaaggc
      661 gactctctcg ctattgtaat taaaaataaa aacggaaatg attacttatc cctcggttac
      721 tacgatcagg acgactacca cattcaaaga ggaattcgta ttaatggtga tagtc
//
LOCUS       DAJVOY010000385          764 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVOY010000385 DAJVOY010000000
VERSION     DAJVOY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.389"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OQL90_004276"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OQL90_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1370800.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFINNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
BASE COUNT          279 a           99 c          151 g          235 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattaa taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa gaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtggtg aacg
//
LOCUS       DAJVOY010000386          748 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVOY010000386 DAJVOY010000000
VERSION     DAJVOY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.390"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>748
                     /locus_tag="OQL90_004277"
     CDS             <1..>748
                     /locus_tag="OQL90_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1370801.1"
                     /translation="LACGTRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATD
                     LWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQE
                     PGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLL
                     KAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKK
                     PPIAASRLAHYNGGASLSFRYLDHKTGETAT"
BASE COUNT          172 a          196 c          237 g          143 t
ORIGIN      
        1 tgctggcctg cggcacgcgg atactgggag tgaaggaata catctgcgat aaacctgagt
       61 gcccccacgt cagatacgtc actaactcat gcggcagccg tgcctgcccg tcctgcggaa
      121 aaaaggccac agacctgtgg atagcgacac agctgaatcg tcttcctgac tgcgactggg
      181 tacacctggt cttcaccctg ccggacacgc tgtggccggt gttcgaaagc aaccggtggc
      241 tgctgaatga cgtgtgccgt ctggcggtgg agaatctgct gtatgccgcc cggaaacggg
      301 ggcaggaacc cggtatcttc tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc
      361 cgcatgtaca tgtgtctgta acctgtggag gtctgaataa gcatggtcag tggaaaaagc
      421 tgagcttcct gaaagacgcg atgcgttcac ggtggatgtg gaatatgcgg cagctgcttc
      481 tgaaagcgtg gtcagagggg ctggcgatgc cggagtcgtt gtcacatatc acgacggaat
      541 cacagtggag aagcctggtg ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga
      601 aaaaaacggc cggcgggagg aatacggcgc gctacctggg tcgttatctg aagaagccgc
      661 cgatagcggc ctcccggctg gcacattaca acggaggggc gagcctgagc ttccgttacc
      721 tggaccacaa aacgggagaa acggcgac
//
LOCUS       DAJVOY010000387          741 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVOY010000387 DAJVOY010000000
VERSION     DAJVOY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.391"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..355)
                     /locus_tag="OQL90_004278"
     CDS             complement(<1..355)
                     /locus_tag="OQL90_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370802.1"
                     /translation="MSIDFTPGIINTYHGNIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIN"
BASE COUNT          218 a           99 c          146 g          278 t
ORIGIN      
        1 tattaatctg attaagtatt gattgtgtct gttgcgtaag tggtgtcgaa cttatcgttt
       61 caatgttatt aataagactt acaagttgaa ccacaacttt tgagtagtct gcatttgtat
      121 atgtggtgtt cgtgtggcat gcattaattt gttggagttg ttgttgcgct aaatcaaatt
      181 gttcagggtt gtatccttct ccagagaatg tggaattaat gggttgttgt tcttcccaat
      241 tatcacaagg ccacttcggt gtgtctggcg ttttaacatt atcggtattt gttgtgcagt
      301 tataaatatt gccgtgatat gtatttatta tacctggggt aaagtcaatt gacataacat
      361 tccttattca tcatctgttt tatgctaagg tttttaaatt ggataattag aacaggaatg
      421 gattttcttc tacacacaat aaatataatg cgttatatct tcctgatata attaccttta
      481 aattcccttt catttccacc cggtaatatt tgtgtataaa aaaagcagtg atagtatcgt
      541 aaaggaaaac gttaacaact atcaaaaagc gctcatttta aaaagttatt atgttctccg
      601 atagtgtgtg aagtaatatt tttgtgatgt tttagatgtg taataatgaa ccaggttaaa
      661 gtgtatttaa aaatgtgttg tattagcgac tgaaatctgc cttttaaaaa ggaggagggt
      721 actgagacaa agagtcattg c
//
LOCUS       DAJVOY010000388          732 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVOY010000388 DAJVOY010000000
VERSION     DAJVOY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.392"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            135..564
                     /locus_tag="OQL90_004279"
                     /pseudo
     CDS             135..564
                     /locus_tag="OQL90_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          182 a          145 c          233 g          172 t
ORIGIN      
        1 attcctgccg gtatcaacgc cgacggcatg tattgccctg aatgcggtac ggttcactgg
       61 ccggatggcg ttattccgcc tttctgatgc ttccccgttt tgccgatatt tttcagcagg
      121 gaaaccgatg gcttaactgg ctggagaagc agccggtgca gatgagccgc ctggaacatt
      181 atgccggtca ggatgaaatt ggcctgcgtt acaacagcca ccggacaaaa cgagaagaaa
      241 accttgtgat gagtggcgac gagtttatgg aaaggttctc ctggcatgtg gcggataagg
      301 ggttccggat ggtgatacgg ggtcctgagt ccggtgaagc ggcgattact ggaagatgtg
      361 gtgtacgcca taacggagac agtgagaaaa acggcgaggc aaatcacaag gagagggatg
      421 tatcagcagt gactgaaggt tgacccgctg aagtgcgagc tgtgcggagg tcagatgcgg
      481 tttacggggc tgaagagggg ttaccgtctg gcagagcagg ttatgatgca tgagccgctg
      541 gctcgaatac ggtggtgcgg ctgagagtcg cagaggggaa gttgcgtcca tgttgagggg
      601 aacggagcaa aaaaccatca gagatacatt gaatcaatca atgtaagcaa tattaagtgt
      661 ctataccgat tacttgatga tcaggttgtg ttgaataaac gcgcattctt cggtgctttt
      721 ttaatctttt gg
//
LOCUS       DAJVOY010000389          710 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVOY010000389 DAJVOY010000000
VERSION     DAJVOY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.393"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..196)
                     /locus_tag="OQL90_004280"
     CDS             complement(<1..196)
                     /locus_tag="OQL90_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCS1370803.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVA"
     gene            complement(201..>710)
                     /locus_tag="OQL90_004281"
     CDS             complement(201..>710)
                     /locus_tag="OQL90_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1370804.1"
                     /translation="SGFELNGKGTSTRPTLTVSNLYGMVAGMAEDLQSLVGGTVVRRK
                     VYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDGAVFPG
                     RIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNTGNFGG
                     FLSINKLSQ"
BASE COUNT          152 a          237 c          169 g          152 t
ORIGIN      
        1 gtgccacaat ctccccctgc atctctgccc gcagccagtc ttccggtgcg atacgaaaat
       61 acgcctccgg ctctgcagaa atattcacac aagggatata ccgctccccc tccggcgttc
      121 tcaccacgaa gccacacgac tccgctggcg cacatcgccg ggcgtgcgcc agaatcgctg
      181 attctgtctg tgtcatggat ttactgagaa agtttattga tggaaaggaa accgccgaaa
      241 ttaccggtat tgttgcgcaa ctcacacccg cgcatacact tgctgcattt gtccttccgg
      301 atatccgtcg ttggtttgtc gaactcgtca gccactgccc tgcccgtgta accacactca
      361 tcagaacggt aggtccacat acaggtattc gccagcatga tacgccccgg gaaaaccgcg
      421 ccatccgtct ccgttggtgt ggccagcaca aaagaggcac tgaccgcgct cagttcgctg
      481 cactgctcga tgcgccagcg gctgatcacc tcctgctccg gatcggcgtc actgtttccg
      541 ttgacgaagt tcaccgcatc cagaaaacgg gcgtaaacct tacgccggac caccgttccg
      601 ccgaccagac tctgcagatc ttccgccatc ccggcgacca taccgtacag gttagaaacc
      661 gtcagcgtgg ggcgcgtact ggtgcctttg ccattcagtt caaaaccact
//
LOCUS       DAJVOY010000390          709 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVOY010000390 DAJVOY010000000
VERSION     DAJVOY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.394"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..355)
                     /locus_tag="OQL90_004282"
     CDS             complement(<1..355)
                     /locus_tag="OQL90_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HCS1370805.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVIAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQA"
     gene            complement(363..566)
                     /locus_tag="OQL90_004283"
     CDS             complement(363..566)
                     /locus_tag="OQL90_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1370806.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
BASE COUNT          200 a          167 c          179 g          163 t
ORIGIN      
        1 tcgcctgcac accgagacgg ttacgacgtc cggcctcctg gcgtttctcc tgtgccagta
       61 cctgataata agcaacatcg gcttgcgctt cctcaagcgc cttttgatag cgcggctggt
      121 cgatggtgaa cagtatctgt ccttttttca ccagctggtt atcatgaaca ttcacctggg
      181 taatgagtcc agaaacgtcc ggcgcgatcg caatgacgtc agcgctaaag cgcgcgtcac
      241 gcgtccaggg ggattcggtg taatagaccc aggcattaaa aattgcgatg aaggccagaa
      301 tgactaatac gaccgtgatg gccgtacggg agaattttct tattagtgtt ttcacttcaa
      361 cctcaaacga acagtcgcga tatcaaataa aacaagcagc aatagagcgc ggtgttgaac
      421 aacgccggat gccagacaaa gtcgtagata cctgttggca caagtacccg gcgcaccagc
      481 cagaaaatcg ccagtgataa aagcaattca aaaaatatcg gtgggaagga cagcccaaac
      541 accacgataa cgggaaacag actcatgttg accttggttg tatagagaga gcaggcgtta
      601 ttattttcag catctgtcgc cgcagagaag ggcatggaaa gccgggcgag agcaacattg
      661 ctgtagattg atatttaata tattagcgta actgttatgc tgttatcta
//
LOCUS       DAJVOY010000391          708 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVOY010000391 DAJVOY010000000
VERSION     DAJVOY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.395"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..311
                     /locus_tag="OQL90_004284"
     CDS             <1..311
                     /locus_tag="OQL90_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1370807.1"
                     /translation="TISQWERDETQPAGKRLFALSQALQCSPTWLLFGDEDKQPGEPI
                     PDNQPAILTEDQKELLQLFDALPESEQKALLSEMRARVENFNKLFEELLKARKRSANK
                     "
     gene            515..667
                     /locus_tag="OQL90_004285"
     CDS             515..667
                     /locus_tag="OQL90_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1370808.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
BASE COUNT          226 a          167 c          138 g          177 t
ORIGIN      
        1 taacaatttc tcaatgggaa agagatgaaa cacagccggc ggggaaaaga ttattcgcac
       61 tgagccaagc gcttcagtgc tcaccgactt ggcttctttt tggggatgaa gataaacaac
      121 caggcgaacc gatcccggat aatcagccag ccattctgac agaagatcaa aaagagttac
      181 ttcaactgtt cgacgcactg cctgagtcag agcaaaaggc cctgttgtca gagatgcgtg
      241 ctcgagttga aaatttcaac aaactttttg aagaactact caaagctcgc aaaagaagcg
      301 caaacaaata atcccctttt ttctccacac cctgtaataa aaagcacaaa ctttcaaaca
      361 cttgtgtttt ttacatcaaa aaaacttagg ttttttatac ataaaatctt gactccacgt
      421 cttaggttat tctaaatttc attcatcaag acaccgcacg gtgttctcag caaacagttc
      481 cactaccccg gcgttaaggg gaaatgaggt cagcatggat actatcgatc ttggcaacaa
      541 cgaatcccta gtgtgtggcg tgtttcccaa tcaggacggc acattcaccg ccatgacgta
      601 taccaaaagc aaaacgttta aaaccgaagc tggcgcacgt cgctggcttg ccaaaaatac
      661 cagctaatcc atttattgga ttaattcaat attctcgctg tagaggta
//
LOCUS       DAJVOY010000392          681 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVOY010000392 DAJVOY010000000
VERSION     DAJVOY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.396"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..353
                     /locus_tag="OQL90_004286"
     CDS             <1..353
                     /locus_tag="OQL90_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1370809.1"
                     /translation="ESDAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITFDAY
                     HMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKAYVA
                     EAISVLAPFFEETL"
     gene            353..681
                     /locus_tag="OQL90_004287"
                     /pseudo
     CDS             353..681
                     /locus_tag="OQL90_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          184 a          153 c          172 g          172 t
ORIGIN      
        1 aggagtccga tgcgccgaac gaagaactgc gtcaggcctg ggagtattac cacacccctc
       61 gcgcgcagta tccaacagca ccgggttacg ctactttgcg cagccttaac cagattatta
      121 cctttgatgc ttaccatatg gcggaagtgt acctgactca gccgatgcag attgtggcgg
      181 gcagccaggc agggagcaaa tggatgagtg acgatctgta cgatcgagca tcaagccaag
      241 ataaacgcta tcacattgtt gaaggcgcaa accatatgga tctttatgac ggcaaagcct
      301 atgtggctga agcaatttca gtattagctc cgttctttga ggaaactctg taatgaaaca
      361 tattcaaatc cgcaatagtg atatggcctg gcatattgct gccaatattc agtttccacc
      421 aaattttgat gagtcaaaac agtatccggc gattatcagc gtccatcctt ttggcagttg
      481 taaggagcag acgtctggca atatctatgg caaagcattg gctgaaaaag gctatctcgt
      541 tctcgcttat gacgccagtt tctagggaga atctggtggg gagcctcgct ggatagaaga
      601 tccgacacag cgcgtagagg atattagccg tgttatcgat tatgcagtaa ctctgccata
      661 tgttaatgct gaacgtattg g
//
LOCUS       DAJVOY010000393          678 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVOY010000393 DAJVOY010000000
VERSION     DAJVOY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.397"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..526
                     /locus_tag="OQL90_004288"
     CDS             <1..526
                     /locus_tag="OQL90_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1370810.1"
                     /translation="LPWLSEADRRLQIKSALPWWLVCRGEIHKFRCVPHLTGRRFEHG
                     VTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVTGFYRVPLSSAPPGDI
                     LLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAF
                     TGICNDLAAASACM"
     gene            424..>678
                     /locus_tag="OQL90_004289"
     CDS             424..>678
                     /locus_tag="OQL90_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1370811.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEG"
BASE COUNT          135 a          189 c          210 g          144 t
ORIGIN      
        1 gctgccctgg ctgagcgagg ctgaccggcg gctgcagata aaaagcgcac tgccctggtg
       61 gctggtctgc cggggggaaa ttcataaatt ccgctgtgtg ccacatctga caggacggcg
      121 ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcatacc atctggcggg
      181 aattgatatg ccggattttc atcgcgagga tgactggtgg cgcaacggcc agaacctgta
      241 cctggacaat atggcggtca ccggctttta ccgggtgccc ctgtcctctg caccgccggg
      301 cgatattctg ctgtgctgct ttggtgcttc ggtaccgaac catgccacca tttactgcgg
      361 caacggtgag ctgcttcacc atctgcctga acaactgagt aaacgggaga ggtattccga
      421 aaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc cactggcacg catctgcctt
      481 cacggggatt tgcaacgatt tggccgccgc ctcagcctgt atgtgaacac ggcagcggaa
      541 gccattcgcg ccctgtcgat gcagatgccg ggctttcgcc gtcagatgaa cgaaggctgg
      601 taccagatac gtattgccgg tgatgacacg gcaccggagg cggtgtatgc ccgtcttcac
      661 gaacagctgg gtgaggga
//
LOCUS       DAJVOY010000394          665 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVOY010000394 DAJVOY010000000
VERSION     DAJVOY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.398"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..>665)
                     /locus_tag="OQL90_004290"
     CDS             complement(<1..>665)
                     /locus_tag="OQL90_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCS1370812.1"
                     /translation="GGNVFFSCLVFVFFYFAILMTVSADMPLVGCVLTLSLLVLLIFM
                     AAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFISTKMLHANS
                     SSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYA
                     ILAFLALLPFLAVAGYIIFEQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYF
                     GIA"
BASE COUNT          268 a          142 c           96 g          159 t
ORIGIN      
        1 agcaatccca aaataataga ttaactgcgc gattatcatt ttacgttgca tttccataaa
       61 ttgctgtaaa ttctcaatat catcatttgc atacacagaa ctatcatacg catttaatat
      121 ttgttcgaag ataatataac cagcaacagc gagaaaaggt aataaagcta aaaatgcgag
      181 gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat gtataccgaa
      241 actggtattg ctccagagaa aactcattac caggctatat aaagtaccat taaaaatacc
      301 aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac tacttgaact
      361 attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa tggccattaa
      421 aatcggcaag aaaaaggtta cccaccagaa ccctttcata gagcaattaa aactaaatct
      481 tacgccgttg agactagtca tcaaggcctg ataacgcagt ccttttgctg ccataaatat
      541 aagcaaaacc aacagtgaca aagtcaaaac acagccaaca agcggcatat ctgctgacac
      601 tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaaaaa aaacattccc
      661 accag
//
LOCUS       DAJVOY010000395          646 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVOY010000395 DAJVOY010000000
VERSION     DAJVOY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.399"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..>646)
                     /locus_tag="OQL90_004291"
     CDS             complement(<1..>646)
                     /locus_tag="OQL90_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCS1370813.1"
                     /translation="DDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSA
                     PNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASFS
                     DASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYESGAH
                     SDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHAL"
BASE COUNT          189 a          143 c          123 g          191 t
ORIGIN      
        1 ttcaatgcat gccagagtgt cgttttataa gccgattcat ttagtgttac accctgctga
       61 gttttgttct gttgcttaaa ccagcctaat tcaagccccg tctgattcca tgtattccag
      121 atccaggccg gtcttattac agtgcgaata ctgtcaaaat cactatgagc accactttca
      181 taactataaa catcttcgcc gtgagaatag acaagcgcgt tagccataat aatattgtca
      241 gtaagataca tctcgccctg agagattaaa cgccaggcga tcccattggt atgatcacca
      301 tagtaatagc gaccatgcgc catcgtatta ctggcatctg agaaactggc aaaactgctg
      361 gcataggaat tattggcaac ctgtaacgta aattcattaa acgtatcgcg ttgcaggttt
      421 tgccgtaaaa cagaagtcag catccagcta tctttcattt caaaataact gttgtcattt
      481 tcgttatctt gttgttgatc cgttttatta ggtgcggaat atttagccat taatgataat
      541 gtcgcatcat cccataacgg gatattgcga taacgaacat ccacagaatt ggtattcatc
      601 tggctggtac gcgaaaaatc acgagagtaa acatcgacat catcac
//
LOCUS       DAJVOY010000396          626 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVOY010000396 DAJVOY010000000
VERSION     DAJVOY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.400"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..394
                     /locus_tag="OQL90_004292"
     CDS             <1..394
                     /locus_tag="OQL90_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1370814.1"
                     /translation="AGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTML
                     FSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEML
                     VGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          155 a          141 c          192 g          138 t
ORIGIN      
        1 ggccggaaag ggtggactgc agattgtgtt gggggcggca gccatcgtgg gctctttctt
       61 cactgccggg gcatcaatgg cgttatgggg ttcagccctg gcagccggtg gtttttctgc
      121 caccacgatg ctgttttcac ttggagccag catgattctg ggcggtgtgg cccagatgct
      181 ggccccgaag gcaaaaacac cggattaccg cgcaacggat aacggcagac agaacacgta
      241 cttttcctcg ctggataaca tgattgccca ggggaacccg atgccggtgc cttacgggga
      301 aatgctggtt ggctcccgcc gtatatccca ggacatcagc acccgtgatg aaggcggggg
      361 cggaaaggtc gtggttatcg ggcgacaggg ataaaacata aaaaaatccc gcagtgatcg
      421 cggagctgcg gggacagaca aatgaagatc aatgtgaagg agttgttttt gttactcggg
      481 caaaaaaaca ctaacgcagc gaaattataa gcgccacagt cagtgtgtga aaatgtgaag
      541 atattcagaa attttattcc gtcatgacgc aggcacccgg tgaggtgcct gttgtttttg
      601 tgagtgaaca attatcacgg taagag
//
LOCUS       DAJVOY010000397          625 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVOY010000397 DAJVOY010000000
VERSION     DAJVOY010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.401"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..233)
                     /locus_tag="OQL90_004293"
     CDS             complement(<1..233)
                     /locus_tag="OQL90_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1370815.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPM"
     gene            340..>625
                     /locus_tag="OQL90_004294"
     CDS             340..>625
                     /locus_tag="OQL90_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1370816.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNS"
BASE COUNT          184 a          147 c          147 g          147 t
ORIGIN      
        1 aacatgggta ttgtcgagca actacagaac ggggtaacgg ctcccaacag agcagccagg
       61 agatagcctc tcccttttct ggcccccatc atctgctgga ttttatggtc tggcaccttt
      121 tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag ctcgacggcg
      181 agaaacacaa acatctctgc ggcatcttgc agcatcgcaa gccaactact catacgtaac
      241 ctcattggat aaatctatat ttccagaata atcgaaatga aattcagatc aagatatttc
      301 tggtattatc gaaatatcaa ccagacagag gggtgtcata tgcagttgga agaagtcgct
      361 aaagcgctga aagagctagg tcacccgacc cgtttgttca tatttaaaca tcttgtcaaa
      421 gccggggaac aagggttgcc ggtcggtgag ttgcaaaagc agcttgggat acctggctcc
      481 acactcagcc accatatatc agcactggta tcggtaggac tggtcaaaca aaaccgtgag
      541 agtcggacgc tgatgtgtgt ttcgcaatac gcaatgctgg aagcgataat tgaattctta
      601 cgcgaggagt gctgcgtaaa tagca
//
LOCUS       DAJVOY010000398          621 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJVOY010000398 DAJVOY010000000
VERSION     DAJVOY010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.402"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            106..>621
                     /locus_tag="OQL90_004295"
     CDS             106..>621
                     /locus_tag="OQL90_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCS1370817.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECS"
BASE COUNT          159 a          167 c          164 g          131 t
ORIGIN      
        1 cctaaatagc tgcgcggaat agtagatccc tttgagggaa cttagcccgg attgtgcgat
       61 ctgatcaatc gccaaatcaa aacaaatcac caaccggact gagcaatgcc gatcatagca
      121 ccaatttccc gtgacgaacg acgcctgatg cagaaagcca tccataaaac acacgataaa
      181 aattatgccc gcagactgac tgccatgctg atgctgcacc ggggcgaccg tgtcagcgac
      241 gttgccagaa cgctctgctg cgcccgttcc tctgttggac gctggattaa ctggttcacg
      301 cagtcgggtg ttgagggact gaaatcatta cctgccgggc gtgcccgtcg ctggccgttt
      361 gagcatatct gcacactgtt acgtgagctg gtaaaacatt ctcccggcga ctttggctac
      421 cagcgttcac gctggagtac agaactgctg gcaataaaaa tcaatgagat aaccggttgc
      481 cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg cggggattgt gtggcgaagg
      541 gctgcgccaa ctctgcgtat ccgtgacccg cataaagatg aaaagatggc agcaatccat
      601 aaagcactgg acgaatgcag c
//
LOCUS       DAJVOY010000399          611 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVOY010000399 DAJVOY010000000
VERSION     DAJVOY010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.403"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            46..>611
                     /locus_tag="OQL90_004296"
     CDS             46..>611
                     /locus_tag="OQL90_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1370818.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAQNDIDAALTGTLKMAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATM"
BASE COUNT          159 a          149 c          185 g          118 t
ORIGIN      
        1 ctctccggaa ggttcttcct gatttaattt atcgcgagaa aaactatgtc tgtagtgata
       61 tcaggtgcgc tgactgatgg cgcaggcatc cccatgtccg gatgccagat tattctgaaa
      121 tcccgtgtaa acacctcaga agtggtgatg cgtaccaggg ctgatgtagt gaccggaaat
      181 aacggtgaat attcgtttga ggcacaggtc ggaaaatatt gcgtgtatct gaaacgggac
      241 tggcgcgacg agtactgtgt tggcgacatt gctgtatacg acgactcaaa gcccggcacg
      301 ctgaatgact ttctgactgc ccccgatgaa ggcgacctga agccggatgt cgtcaaacgc
      361 tttgaggaaa tggtggcgca ggcgcagcag agcgccgggg ccgcagccgg aaacgcacag
      421 cagacggcgc aggatgtggc ggcagccgca ggttatgccc gcgcagcaga acaggcccaa
      481 aatgacattg atgctgcgct gaccggcact ctgaaaatgg ctaaccatct gtcagaaatc
      541 gcagcagcag gcgaaaaggc acaacagaag tcccgggata atctggggct gaaaagtgcg
      601 gccacgatgg a
//
LOCUS       DAJVOY010000400          602 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVOY010000400 DAJVOY010000000
VERSION     DAJVOY010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.404"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>602
                     /locus_tag="OQL90_004297"
     CDS             <1..>602
                     /locus_tag="OQL90_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1370819.1"
                     /translation="PGLAMRRIADLHAGEAKTDARDAAIIAEAARTLPHALRTLKLAD
                     EQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRY
                     PSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLA
                     LQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRT"
BASE COUNT          127 a          201 c          158 g          116 t
ORIGIN      
        1 tccctggact ggccatgcgc cgcatagccg acttacacgc cggtgaagct aaaactgatg
       61 ctcgtgacgc tgccatcatt gccgaagctg cccgtaccct gcctcacgcg ctacgcacgc
      121 tgaaactggc tgacgagcaa atcgccgaac tctccatgct ctgcggcttc gatgatgatc
      181 ttgccgcaca gacaacgcag gccagcaacc gtatccgcgg ccttctgacc cagatacatc
      241 cggcactgga gcgcgttctc ggtccgagac ttgagcaccc ggcggtactc gatcttctcc
      301 agcgatatcc ctcaccagaa aaactcgctt cgctgggtga gaagaagctg gcagcccagc
      361 tctgcaaact tgcgcctcgt ctgggtaaac gccttgcagc agacatagct caggcactgg
      421 ccgaacaaac cgtcgtcgtt cccggcacga atgccgctgc cgtagtactg ccacgtctgg
      481 cactccagct catcacgctg cgtaagcaaa gagacgaggt ggcgcttgag gtagaacagc
      541 gagttcttgc tcaccctctt tacccggtcc tgaccagtat gcccggagtc ggtgtcagga
      601 cc
//
LOCUS       DAJVOY010000401          599 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVOY010000401 DAJVOY010000000
VERSION     DAJVOY010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.405"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            57..578
                     /locus_tag="OQL90_004298"
     CDS             57..578
                     /locus_tag="OQL90_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370820.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYKHRT
                     EGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQ
                     KLINGIWYNVASI"
BASE COUNT          176 a          120 c          146 g          157 t
ORIGIN      
        1 attcaattgc tttcggggat aacgatacag gtataaaaca aaacggcgac ggcattctgg
       61 atgtttatgc gaatggacag catgtatttc gtttccagaa tggtgtggcg atagcgttaa
      121 aaaatattca ggccggaaat gctaaaaaat tcacgttatc cagcgccaac aactccacga
      181 aaaatgcagc gttttatttg tggggtaatc catccaggcc tgttgttgca gagcttggtg
      241 atgattccgg ctggcatttc tacagccaga gaaataccga tggtagtatc acattcgctg
      301 taaacggaca ggtaattccg ttaaattacg gaaacttcga tgcccgctat aaacatcgaa
      361 ctgagggggt acaggatgtg cggtatggtt atgaaatgta ttacaaccct ggcagcaaca
      421 ccgtttcatg gacgtttcgc tcaccttcgg gacacgggtt gtcagggata tcgatatcgg
      481 ataccggccg taactcagcg gataacgtca acggtgtgta ttaccgaccg ctgcaaaaac
      541 tgattaatgg catctggtat aacgtagcga gtatttaaca atgttgcatt taaaaaata
//
LOCUS       DAJVOY010000402          598 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVOY010000402 DAJVOY010000000
VERSION     DAJVOY010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.406"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
BASE COUNT          219 a          112 c          118 g          149 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccat actcctccgg cagaagcaga aaaagcttat
       61 tatgcttcca tcggaaacga tgatctggca gcctgagttc acagataaaa cactctccag
      121 gaaacccggg gcggttcaag gactctatca tcgccaatca tcagacattg aaatagacga
      181 cactgagatg acgcttacaa ttcttttggt ttctgtaata agtaatctga catcccctgt
      241 gcccagaaga ggagagggat atcaagccca agggttaata attgatacta cggttaaaat
      301 tgccttcagt aaaggaaacg tgattaaatt caggggattt cagagtgaaa accatacaac
      361 atattaaaat ctgttcattt agctataata catacaagaa agtgtccagt tagataatgt
      421 tatctaaata accacagata aaaacgcaca taattggggg ggggatgaaa atacctgaag
      481 cagtaaatca tattaatgtt cagaataata ttgatcttgt tgatggaaaa acgaatccga
      541 ataaagcaac aaaagcatta caaaaaaata tattgcgcgt aacaaactca tcttcttc
//
LOCUS       DAJVOY010000403          569 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJVOY010000403 DAJVOY010000000
VERSION     DAJVOY010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.407"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>569
                     /locus_tag="OQL90_004299"
     CDS             <1..>569
                     /locus_tag="OQL90_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1370821.1"
                     /translation="ETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLGDTPLV
                     DFTRDRLHRETLRRFGRYELSTAYTPAGQLQSQHLNSLQYDRDYTWNDNGELIRISSP
                     RQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRI
                     ARDAHYLYRYDRHGRLTEKTDLIPEGGIR"
BASE COUNT          127 a          203 c          157 g           82 t
ORIGIN      
        1 atgagaccag acatgcgtac aacgcgcagg gactggcgaa ccgctgtata ccggacagcc
       61 tgcccgccgt ggaatggctg acctacggca gcggctggct ggcaggtatg aagctcggcg
      121 acacaccgct ggtggatttc acccgcgacc gcctgcaccg ggaaacgctg cgccgcttcg
      181 gccgttatga actctccacc gcttacaccc ctgccgggca gttacagagc cagcacctga
      241 acagcctgca gtatgaccgc gattacacct ggaacgacaa cggcgaactc atccgcatca
      301 gcagcccgcg ccagacccgg agttacagct acagcaccac cggcaggctg accggcgttc
      361 acaccaccgc agcgaacctt gatatccgca tcccgtatgc cacagacccg gcaggtaacc
      421 gcctgcccga cccggagctg cacccggaca gcaccctcag catgtggccg gataaccgta
      481 tcgcccgtga cgcgcactat ctttaccggt atgaccgtca cggcaggctg acagagaaaa
      541 ccgacctcat cccggaaggg ggtatccgc
//
LOCUS       DAJVOY010000404          569 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVOY010000404 DAJVOY010000000
VERSION     DAJVOY010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.408"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            52..569
                     /locus_tag="OQL90_004300"
                     /pseudo
     CDS             52..569
                     /locus_tag="OQL90_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          186 a          138 c          138 g          107 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccaagt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc atggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatg
//
LOCUS       DAJVOY010000405          553 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJVOY010000405 DAJVOY010000000
VERSION     DAJVOY010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.409"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            171..>553
                     /gene="bluF"
                     /locus_tag="OQL90_004301"
     CDS             171..>553
                     /gene="bluF"
                     /locus_tag="OQL90_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCS1370822.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQ"
BASE COUNT          160 a          114 c          113 g          166 t
ORIGIN      
        1 tgcatcattt aactgacaat ctatacgaga tttttcgcca actagtgttc aactttttcc
       61 attttttatc aatgtaaaga aacggttatg ttttggtatt aaaagaaaga cacaagaaga
      121 tatgtctgtt accgtcttac tctcgcctca cccattaccc tggattgtta atgcttacca
      181 cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc gaagaaatgg
      241 tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta ctgtttaatg
      301 gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg atatatcggg
      361 ctatatgcca ggatccacgg cactataata ttgttgagct gctgtgcgat tacgcgcctg
      421 ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac gagcgagatg
      481 acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact tatgatgaca
      541 gagcgctaca att
//
LOCUS       DAJVOY010000406          547 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVOY010000406 DAJVOY010000000
VERSION     DAJVOY010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.410"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..520
                     /locus_tag="OQL90_004302"
     CDS             <1..520
                     /locus_tag="OQL90_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1370823.1"
                     /translation="SLLKWLATHPCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYH
                     ISPQAVILEITEEQAFSNAESSMYNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADI
                     IKIDGVFVKDIVTNTLDAMIVRSITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQ
                     GYLIGRPQPLAD"
BASE COUNT          145 a          119 c          145 g          138 t
ORIGIN      
        1 atccttgttg aagtggctgg caacacaccc ttgcgacaaa aaaggaccgc gcttttcagt
       61 caatttaatg ccgctcacgc tgctgcaaaa gaatattgcc gggcggatta ttcgtctgtt
      121 taagcgttat cacatctccc cgcaggcggt cattcttgag atcaccgagg agcaggcgtt
      181 ttctaacgca gaaagcagca tgtacaacat cgagcagctg cataagtttg gtttccggat
      241 tgcgattgat gactttggca ccggctatgc caactacgaa cggttaaagc gtttgcaggc
      301 tgatatcatc aaaattgatg gcgtctttgt gaaagatatt gtcacgaaca cgctggatgc
      361 gatgattgtg agatcaatta ccgatctggc gaaagcgaag tcattgagtg tggtcgcgga
      421 gtttgtcgag acgcaacagc agcaggcgct attgcataag ctcggggtgc aatatctgca
      481 agggtatttg attggtcgcc cgcagccatt agctgattaa caggcgtaaa aaaaacgggg
      541 aattatc
//
LOCUS       DAJVOY010000407          538 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVOY010000407 DAJVOY010000000
VERSION     DAJVOY010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.411"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>538
                     /gene="fimZ"
                     /locus_tag="OQL90_004303"
     CDS             <1..>538
                     /gene="fimZ"
                     /locus_tag="OQL90_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCS1370824.1"
                     /translation="MDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDL
                     IIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQ
                     NDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNK
                     EIADKLLLSNKTVSAHKSN"
BASE COUNT          175 a          100 c          101 g          162 t
ORIGIN      
        1 atggatactc atcctatcat cagaatgtct attgaagttc tgttgcaaaa aaacagtgaa
       61 ttgcagattg tcctgaaaac ggatgattac cgcataacca tcgattatct ccgaacccgt
      121 cctgttgatt taatcattat ggatatagac ttgcccggaa cagacggttt taccttcctg
      181 aaaaggatca aacaaatcca gagcacagtg aaagtgttat ttttatcatc gaaatcagaa
      241 tgcttttatg ctggcagagc gatacaagct ggtgctaacg gttttgtcag taaatgcaat
      301 gatcagaatg atatttttca tgccgttcag atgatcctct ccggatacac gttttttccc
      361 agcgaaacgc ttaactatat aaaaagcaat aaatgtagta cgaatagttc aacggtcact
      421 gtgctatcta atcgtgaagt gaccatatta cgttatctgg ttagcggatt atctaataaa
      481 gaaattgccg ataagttatt gcttagcaat aaaacagtta gtgcgcataa atctaata
//
LOCUS       DAJVOY010000408          528 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVOY010000408 DAJVOY010000000
VERSION     DAJVOY010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.412"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..325
                     /gene="dsbG"
                     /locus_tag="OQL90_004304"
     CDS             <1..325
                     /gene="dsbG"
                     /locus_tag="OQL90_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1370825.1"
                     /translation="SGKVQLRTLLVGVIKPESPATAAAILASKDPAKTWQQYKASGGK
                     LKLNVPANVSTEQMKVLSDNEKLMDDLGANVTPAIYYMSKENTLQQAVGLPDQKTLNI
                     IMGNK"
BASE COUNT          191 a           98 c          114 g          125 t
ORIGIN      
        1 ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc
       61 gacagcagcg gcaattcttg cctccaaaga tcccgcaaaa acctggcaac aatataaagc
      121 ctctggtggc aagcttaagc taaacgtgcc tgcaaacgta agtacagagc aaatgaaagt
      181 gttaagtgac aatgagaaac tgatggacga tctgggggca aatgtcacgc cggctatcta
      241 ttacatgagt aaggaaaata cgctacaaca ggccgtgggg ttgcccgatc agaaaacgct
      301 taatatcatt atggggaata aataaaaggt aattcatgga tggttgaact atatctctgc
      361 ttttcaacca tcctaaaaag catgaattgc tcgctctgcc ctaagtggct gtgctatttt
      421 agaaaaactt aatcctgagt taagcaaaaa aacataatca ataaaaatat atacgcagaa
      481 aaacattgat taagtgaata tatcatggaa gaaaaatata accggagt
//
LOCUS       DAJVOY010000409          519 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJVOY010000409 DAJVOY010000000
VERSION     DAJVOY010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.413"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            240..509
                     /locus_tag="OQL90_004305"
     CDS             240..509
                     /locus_tag="OQL90_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370826.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
BASE COUNT          135 a          120 c          140 g          124 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg accaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgtt
//
LOCUS       DAJVOY010000410          515 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJVOY010000410 DAJVOY010000000
VERSION     DAJVOY010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.414"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            235..515
                     /locus_tag="OQL90_004306"
                     /pseudo
     CDS             235..515
                     /locus_tag="OQL90_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          132 a          136 c          126 g          121 t
ORIGIN      
        1 tccccgccag cccggctaag atggaggcac ggcacagccc gcacacgacg ctgaggctac
       61 agagctgaaa acccacaagg ccatgccccg catggccggg taaagcggag ccggcggcga
      121 cttcctttgt gctctgtccg ggaccggcag gaaaaaggcg gggataatga gatggtcacc
      181 cccaccctaa cagcagtacg ctgttaaggt gaatccctta acggagagac catcatacag
      241 aacatcacac ttattggtat cgatctcgga aagcattcct tccacattca ttgtcaagac
      301 aagtctggta aggctcttct gcgtaaaaaa tttacccagt actaaactga tgcagtttct
      361 ggcaacatgt ccgccctcag ttgttgtaat ggaagcctgt gcgggtgcac actttttggc
      421 tcgccgtatc tctgaatttt ggacatgaaa ctaagctgat ttctccacag ttcgttcgcc
      481 cgtttgtcaa aagtaataag aatgattttg ttgat
//
LOCUS       DAJVOY010000411          514 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJVOY010000411 DAJVOY010000000
VERSION     DAJVOY010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.415"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..279
                     /locus_tag="OQL90_004307"
     CDS             <1..279
                     /locus_tag="OQL90_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS1370827.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          125 a          115 c          139 g          135 t
ORIGIN      
        1 agcctgaacg tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac
       61 gcgctgcgat cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt
      121 ggagaaagcg cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag
      181 ccggaggact ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg
      241 catgaactgc tgccctggaa cttctcagct gtaaaataat ccttccgcta cgtacttctc
      301 tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc
      361 atgtgtttta tggatggctt tctccatcag gcatcgttcg tcacgggaaa ttggtgctat
      421 gatcggcatt gatcagtccg gttggtgatt tgttttgatt tggcgattga tcagatcgca
      481 caatccgggc tgagttccct ctaagtgatc tagg
//
LOCUS       DAJVOY010000412          514 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJVOY010000412 DAJVOY010000000
VERSION     DAJVOY010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.416"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            98..>514
                     /gene="ytfB"
                     /locus_tag="OQL90_004308"
     CDS             98..>514
                     /gene="ytfB"
                     /locus_tag="OQL90_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCS1370828.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTM"
BASE COUNT          142 a          136 c          134 g          102 t
ORIGIN      
        1 gtatgaatat aataaaggcg ggtttaccac ggacatcata gcgttagctt attcataggc
       61 tatgattgag gaacaagacg cggagcagga ggaaaccatg cccgggcgct ttgaactaaa
      121 accaaccctg gagaaagtct ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc
      181 gccgatgcat cgtcggggga tcattattgc cgccattgta ttggtggtcg gatttctgct
      241 tccatctgat gatacgccca acgcgccggt ggtgacgcgt gaagcgcagt tagatattca
      301 gtcgcaatca caaccgccga cagaagaaca gctgcgggcg caattagtca caccacaaaa
      361 tgatcctgat caggtggctc cggtcgctcc agaacctata caggaaggcc agccagaaga
      421 gcagccgcaa actacccaaa cgcaaccatt ccagccagat agcggaattg ataatcaatg
      481 gcgctcttac cgtgtcgaac ccggtaaaac gatg
//
LOCUS       DAJVOY010000413          513 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJVOY010000413 DAJVOY010000000
VERSION     DAJVOY010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.417"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>513
                     /locus_tag="OQL90_004309"
     CDS             <1..>513
                     /locus_tag="OQL90_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCS1370829.1"
                     /translation="VNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSL
                     GPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLD
                     EGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRV
                     LTGLVDRFGRT"
BASE COUNT           89 a          163 c          169 g           92 t
ORIGIN      
        1 gtcaatcccc tgctcggtgc aaaggtcctt cccggtgaaa ccgacatcgc cctgcccggc
       61 ccgctgccgt tcatcctctc ccgcacctac agcagttacc ggacaaaaac gcccgcgccg
      121 gtggggagcc tcggccccgg ctggaaaatg cctgcggata tccgcttaca gctgcgcgat
      181 aacacactga tactcagtga taacggcggc agaagcctgt attttgagca cctgtttccc
      241 ggtgaggacg gttacagccg cagcgagtca ctgtggctgg tgcgcggcgg cgtggcgaaa
      301 ctggatgaag gtcaccggct ggccgcactc tggcaggcgc tgccggaaga actccgctta
      361 agtccgcatc gttatctggc gacaaacagt ccgcaggggc cgtggtggct gctcggctgg
      421 tgtgagcggg tgccggaagc ggatgaggtg ctgcctgcgc cgctgccgcc gtaccgggta
      481 ctgaccgggc tggtggaccg cttcgggcgc aca
//
LOCUS       DAJVOY010000414          511 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJVOY010000414 DAJVOY010000000
VERSION     DAJVOY010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.418"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>511
                     /locus_tag="OQL90_004310"
     CDS             <1..>511
                     /locus_tag="OQL90_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCS1370830.1"
                     /translation="RGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNVLSK
                     EPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKLKTL
                     RENARTWLLKYPEHAITGLLASALDKAGEAQDNARAALRMLTENGHQPLLQEIARRYN
                     QPEVTDAVNA"
BASE COUNT          131 a          146 c          122 g          112 t
ORIGIN      
        1 agaggggaga ttattctgcg ctgatcagcg aatttaaaac cttctggcac tcctatcaac
       61 gggaatggca gctttacatg ttagccgccc tgcctatcga aaaaacagcg caggcgtgga
      121 atgtcctcag taaagagcca cacgtcggtg tcgagtttgt catgacgcac ctgcaactcg
      181 ctgggttaca gggttttatt cactcctttt cacgctatcc acaagaagct ctacctgtag
      241 cacaatattt tgcggctatc gaattagcgc cactgattgc ccgtgccttc aacaaactca
      301 aaacgctacg cgaaaacgcc cgcacctggc tgttgaaata cccggaacat gccattaccg
      361 gcctgctggc ttcggcgctc gacaaagccg gtgaagcaca ggataacgcc cgcgctgcct
      421 tgcgtatgct taccgaaaat ggtcatcagc cattactgca agaaatcgcc cggcgttata
      481 accagccgga agtaaccgac gcggtgaacg c
//
LOCUS       DAJVOY010000415          502 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJVOY010000415 DAJVOY010000000
VERSION     DAJVOY010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.419"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..>502)
                     /locus_tag="OQL90_004311"
     CDS             complement(<1..>502)
                     /locus_tag="OQL90_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1370831.1"
                     /translation="FHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFSNAR
                     VIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQT
                     SESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVLRRS
                     LGLRAEK"
BASE COUNT          117 a          145 c          130 g          110 t
ORIGIN      
        1 ttttttccgc acgcaacccc agcgaacgac gtaatactgc ttccggtagc gcgtccgcca
       61 cctgattgcg gaccgcccac caggataatt cagccagaga taattcacgc tcctgcgtac
      121 cgcttattgc gtgaccgatg acgtcaatca tccatgctga caggttttga tgagcaagtt
      181 gctcgagtga ttcggatgtc tggtcacgca actggttgtc gcagtgccag cacaacacca
      241 ttgcgccggt accataacgg tgaatgacgg tttcgctgtg atgataatcg ccgtgtggcc
      301 actggcagga tttaatatgg cgcaacagcc agtcagacaa tgcaccagca ccaccagcag
      361 cacgaatcac ccgtgcgtta ctgaaaaacg gcagcaatgt tttgtcttcc actagcggct
      421 ggcgaacggc aggaacgacc ccggacggca gattacgcat gcttttcggt tccggctcca
      481 ccagtacccg ggtattgtgg aa
//
LOCUS       DAJVOY010000416          483 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJVOY010000416 DAJVOY010000000
VERSION     DAJVOY010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.420"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..462
                     /locus_tag="OQL90_004312"
     CDS             <1..462
                     /locus_tag="OQL90_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1370832.1"
                     /translation="VGAYSKSDRTNEQAGDGYGDNAEVWSLAAKYDANNIYAAMMYGE
                     TRNMTVLANDHFANKTQNFEAVVQYQFDFGLRPSLGYVYSKGKDLYARDGHKGVDADR
                     VNYIEVGTWYYFNKNMNVYTAYKFNLLDKDDAAITDAATDDQFAVGIIYQF"
BASE COUNT          145 a          100 c          108 g          130 t
ORIGIN      
        1 gtaggtgcat atagcaaatc tgatcgtact aatgaacaag ctggtgacgg ctacggtgat
       61 aacgctgaag tgtggtcatt agcagccaag tatgatgcaa ataatatcta tgcagcaatg
      121 atgtacggtg aaacccgcaa catgaccgtt ttggctaatg atcattttgc aaataaaacc
      181 caaaactttg aagctgttgt acagtatcag ttcgacttcg gtttacgtcc gtctttaggc
      241 tacgtatatt ccaaaggcaa agatctttat gctcgtgatg gacataaagg tgttgatgct
      301 gaccgcgtaa attatatcga agttggtacc tggtactact tcaataagaa catgaacgtc
      361 tacacagcat acaaatttaa cctgctggat aaagacgatg cagcgattac cgatgccgca
      421 actgatgacc agtttgcagt tggtatcatc taccagttct aatcagccaa cgcgccgcgg
      481 cgt
//
LOCUS       DAJVOY010000417          480 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJVOY010000417 DAJVOY010000000
VERSION     DAJVOY010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.421"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(1..>480)
                     /gene="corA"
                     /locus_tag="OQL90_004313"
     CDS             complement(1..>480)
                     /gene="corA"
                     /locus_tag="OQL90_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1370833.1"
                     /translation="LSRVIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNF
                     LVRKARLPGGQLEQAREILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIF
                     SVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT          116 a          138 c          119 g          107 t
ORIGIN      
        1 ttacaaccag ttcttccgct taaagtacag atacggtgcc aggcccgcga ggatcataaa
       61 gataatcgcg ccagggtagc cgaagctcca cttcagttct ggcataaact caaagttcat
      121 gccatagctg gaagcaacga gcgtcggcgg caggaatacc acggatacca ccgagaagat
      181 tttgatgatg cggttctgct cgatgttgat aaaacccatc gccgcctgca tcaggaagtt
      241 caccttctgg aacagagatt cgttatgcgg cagcagggat tcgatatctc gcaggatttc
      301 acgcgcctgc tccagttgtc cacccggtaa gcgtgcttta cgtaccagga agttgagcgc
      361 gcgctgggta tccatcagac acaggcgtac tttccagccg atatcttcca gttccgccag
      421 cgtggagagc gcctcatcgt actcatcgcc ctgatgccct tccataatca cccggctcaa
//
LOCUS       DAJVOY010000418          477 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJVOY010000418 DAJVOY010000000
VERSION     DAJVOY010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.422"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>477
                     /locus_tag="OQL90_004314"
     CDS             <1..>477
                     /locus_tag="OQL90_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1370834.1"
                     /translation="PKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDYTIFFYNPN
                     IHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGIRCTMCFDM
                     RFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWDYNWRKQG"
BASE COUNT          121 a          110 c          132 g          114 t
ORIGIN      
        1 gcccaaattg gagctcccaa acggggcaaa caaactacta ctgcactctt gctgtgctcc
       61 atgttccggt gaggtgatgg aggcgcttca ggcctcggga atcgactaca ccatcttttt
      121 ctacaacccg aacattcatc ctcagaaaga gtatttaatt cgtaaggatg agaatattcg
      181 ctttgctgaa caacacggcg tgccgtttat tgatgctgat tacgacacag acaactggtt
      241 tgaacgagcc aaaggaatgg aatgggagcc cgaacgaggg atccgttgca ccatgtgttt
      301 tgacatgcgt tttgagcgga cagcgctgta cgccgctgaa aatggtttca gtgtgatcag
      361 cagttcactg ggcatttcac gctggaaaaa tatgcagcag gttaacgact gtgggcggcg
      421 agccgtcgcg cattatccgg gcatggtgta ctgggattat aactggcgca agcaggg
//
LOCUS       DAJVOY010000419          468 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJVOY010000419 DAJVOY010000000
VERSION     DAJVOY010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.423"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>468
                     /locus_tag="OQL90_004315"
     CDS             <1..>468
                     /locus_tag="OQL90_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1370835.1"
                     /translation="YHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRER
                     DLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVETATGELAK
                     TQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLAS"
BASE COUNT          101 a          132 c          160 g           75 t
ORIGIN      
        1 ggtaccatta cgacagtcag caccggctgg tgcactacac gcggacacaa tatgcagagc
       61 cgctggtcga aagtcgctat ctttacgacc cgctgggccg cagggtggca aaacgggtgt
      121 ggcggcgtga acgtgacctg acgggctgga tgtcgctgtc acggaaaccg gaagtgacct
      181 ggtacggctg ggacggcgac cggctgacca caatacagaa cgacaggagc cgcatccaga
      241 cgatttatca gccggggagc ttcacgccac tcatcagagt tgaaaccgcc accggtgagc
      301 tggcgaaaac gcagcgccgc agcctggcgg atacccttca gcagtccggc ggcgaagacg
      361 gtggcagtgt ggtgttcccg ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa
      421 tcctggctga ccgggtgagt gaggaaagcc gccgctggct ggcatcgt
//
LOCUS       DAJVOY010000420          456 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJVOY010000420 DAJVOY010000000
VERSION     DAJVOY010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.424"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..276
                     /locus_tag="OQL90_004316"
     CDS             <1..276
                     /locus_tag="OQL90_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCS1370836.1"
                     /translation="LKDKAGTDTRFAAEIVDISRGGMRVRLVDNGAIAFIPAPFLHAM
                     RDELVCSQENGTVQIKGETVYKVTDVIDVTIAEVRMETRSIIARPVA"
     gene            complement(354..456)
                     /locus_tag="OQL90_004317"
                     /pseudo
     CDS             complement(354..456)
                     /locus_tag="OQL90_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          117 a          112 c          101 g          126 t
ORIGIN      
        1 ctgaaagaca aagccgggac cgacacccgt ttcgcagcgg aaattgtcga tatcagccgt
       61 ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc
      121 ttacacgcta tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa
      181 ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga agtccgcatg
      241 gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ctattcctca
      301 tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat tttttaactg
      361 actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata tataaaaaaa
      421 caatgcgttt agccgccgtt aatttcatgt aagtca
//
LOCUS       DAJVOY010000421          451 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJVOY010000421 DAJVOY010000000
VERSION     DAJVOY010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.425"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            11..>451
                     /locus_tag="OQL90_004318"
     CDS             11..>451
                     /locus_tag="OQL90_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1370837.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKW"
BASE COUNT          129 a           85 c          138 g           99 t
ORIGIN      
        1 aaggttattt atgatttacc caacaaatac aggcaaaagc ggggaacacc ttcgtctcac
       61 cacgctggaa agtgtctgga ttcagggaaa actgcgcatg tgggggcgct ggtcgtatat
      121 tggcggcggt aagacgggga atatgttcaa ccagttgttg gcctctaaaa agctgacaaa
      181 aacggcaatt aacgaggcgc tccggaggat gaaaaaagca ggtctgaaca agtctgaact
      241 tgaggctttt ttgcgggata tgattaacgg taagcaaaag agctggctgg cgcattgtac
      301 tgatgcagag gcgttatgta ttgatcgggt cataagtgag gtgctggcag agcatccagg
      361 attgattagc gtccttcggc aacggtatga ggggcggggg atgaccaaac gcaaaatggc
      421 tgaactgctg aatgatgcac acccgaaatg g
//
LOCUS       DAJVOY010000422          447 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJVOY010000422 DAJVOY010000000
VERSION     DAJVOY010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.426"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            35..447
                     /locus_tag="OQL90_004319"
                     /pseudo
     CDS             35..447
                     /locus_tag="OQL90_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          101 a          115 c          121 g          110 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcac
//
LOCUS       DAJVOY010000423          447 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJVOY010000423 DAJVOY010000000
VERSION     DAJVOY010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.427"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..332)
                     /locus_tag="OQL90_004320"
     CDS             complement(<1..332)
                     /locus_tag="OQL90_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1370838.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPE"
BASE COUNT          140 a           95 c          108 g          104 t
ORIGIN      
        1 ctttccggta aaaataaaaa cggtaaatag agcgcaatat cggaaataac atcgccagtt
       61 tcattcaaaa tcgcccccag acgtgtttgc tgattgcact cacgcgccaa catgccatcc
      121 agcgcattga gcgccatacg gataaaaagc acgatgggca atagcaaaaa gaggatgggt
      181 tgtgccacca acaccaacag caatccggta aaaagagaaa gcgccagtgc cgtaagagtg
      241 atgtgattcg ctgtaacgtg gtgcttataa agccaaaaca tcgtcggcct taacagcgac
      301 tgaaagagcg gttttatctg gtagagcgtc atttatcatt ccgtgatatt cagtttgctc
      361 tatggtgcat caattgtgat ggcggagata tcacaacgcg gagcatcaac gtaaattcgg
      421 aatatatcaa gatgtggtgg atggttt
//
LOCUS       DAJVOY010000424          447 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJVOY010000424 DAJVOY010000000
VERSION     DAJVOY010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.428"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..>447)
                     /gene="ipaH3"
                     /locus_tag="OQL90_004321"
     CDS             complement(<1..>447)
                     /gene="ipaH3"
                     /locus_tag="OQL90_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCS1370839.1"
                     /translation="TSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNH
                     HSEETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDYHG
                     PRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT          100 a           90 c          127 g          130 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatccgtgg gccgtggtag tccggttggg cggtttgttg cgacagagac
      181 tccctgatcc gtgaggacag aggattgtct tcgaggatga tagtgcaggt cggatcaagg
      241 ctaagtatat tttccggaat gtgtgtgatg cgattctcgc ggcaccggaa aaatatctcg
      301 gtttcctctg agtgatgatt tcttacaggt acggctggta ggctttccag aagattagta
      361 cttacatcga gcgcttccag tgattcaggt aactcaggga gaaatgtcag ctggttattt
      421 cttactgaga gcacttccag cgatgta
//
LOCUS       DAJVOY010000425          441 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJVOY010000425 DAJVOY010000000
VERSION     DAJVOY010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.429"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>441
                     /locus_tag="OQL90_004322"
     CDS             <1..>441
                     /locus_tag="OQL90_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1370840.1"
                     /translation="NGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLL
                     NQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKAL
                     FMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKG"
BASE COUNT          115 a          116 c          120 g           90 t
ORIGIN      
        1 aatggcatca tcgttggtcg tgaccgactg gcacgtcttc gtaaggagct aaggctacgc
       61 tgtaagcaga aacgcaagtt cagagcgact acgaacccga accacaatct gccagttgcg
      121 ccaaatctgc tgaaccagac gttcgctcct acagcaccaa atcaggtctg ggtggcggac
      181 ctgacgtatg ttgccacaca ggagggatgg ttgtacctcg ctggcatcaa agatgtttat
      241 acgtgcgaaa ttgtcggcta cgccatggga gagcgcatga caaaagagct gacaggtaaa
      301 gccctgttta tggcgctcag gagccagcgc ccacctgccg ggctaatcca ccactctgat
      361 cgaggttcac agtactgcgc atacgattac cgggtcatac aggagcagtc tggtctgaaa
      421 acatcaatgt cgcgtaaagg t
//
LOCUS       DAJVOY010000426          440 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJVOY010000426 DAJVOY010000000
VERSION     DAJVOY010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.430"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            7..366
                     /locus_tag="OQL90_004323"
     CDS             7..366
                     /locus_tag="OQL90_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1370841.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMEFGERTVIK
                     NPEYFSTYMQEELKALV"
BASE COUNT          150 a           75 c           98 g          117 t
ORIGIN      
        1 tattagatgc aaattaaggt catatatagc cttatcgata acatggttaa ttttaaggat
       61 aagaatatgc ctgctgtaat agataaagcc ctggatttca ttggtgccat ggatgtatca
      121 gcgccaacac caagttcgat gaatgaaagc acagcgaagg gaatctttaa atatttaaaa
      181 gaactgggag tacccgcgag tgccgctgat attacggcgc gagctgacca ggaaggctgg
      241 aaccctgggt tcacggaaaa aatggttgga tgggcaaaaa aaatggagtt tggtgaacgc
      301 actgtaatta aaaatcctga atacttttca acatatatgc aggaagaact aaaagcactg
      361 gtctgagttt aatttatatc agcataaatg ggtcaggacg cttttaatca catataaaaa
      421 gcgtcctttt tctcacaatc
//
LOCUS       DAJVOY010000427          434 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJVOY010000427 DAJVOY010000000
VERSION     DAJVOY010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.431"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..>434)
                     /locus_tag="OQL90_004324"
     CDS             complement(<1..>434)
                     /locus_tag="OQL90_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1370842.1"
                     /translation="AYGSANKTIIKDKGTMSVLTNAKADATRIDNGGVMDVTGNATNT
                     IINGGTQNINNHGIATGTNINSGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNIS
                     AGGSLIVYTGGIAHGVNQETGSALVANTGAGTDIEGYNKLSH"
BASE COUNT           89 a          116 c           88 g          141 t
ORIGIN      
        1 aagtgagaga gcttgttgta tccttcgata tcagtccctg cgcccgtgtt ggcaactaaa
       61 gcactgcccg tctcctggtt aaccccatgt gctataccgc cggtatagac aatcagcgag
      121 cctccggcgc taatattgct gccagttgcc gtaccatctt tctcaacaac ctgccggctc
      181 ccggtggaaa tatttgtcgt gtcagctttc ccgccgctct tgatattttg tgttccgctg
      241 ttgatattgg tacccgtggc aataccatga ttattaatat tctgtgtacc accattgatt
      301 atggtattgg tcgcgtttcc ggtaacatcc attaccccgc cgttatctat tcgggtcgca
      361 tcagctttag cattcgttaa aactgacatt gttcctttat ctttaataat cgtcttattt
      421 gccgaaccat atgc
//
LOCUS       DAJVOY010000428          423 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJVOY010000428 DAJVOY010000000
VERSION     DAJVOY010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.432"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
BASE COUNT          136 a           66 c           74 g          147 t
ORIGIN      
        1 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
       61 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      121 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      181 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat
      241 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      301 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      361 gcatattgga gcgtgatcaa ttttgatcag ctgtgaaggt ggctgatgtg tagtgataca
      421 aaa
//
LOCUS       DAJVOY010000429          419 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJVOY010000429 DAJVOY010000000
VERSION     DAJVOY010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.433"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..395
                     /locus_tag="OQL90_004325"
     CDS             <1..395
                     /locus_tag="OQL90_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1370843.1"
                     /translation="IARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAA
                     IGSDAVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPL
                     RDFPKWLAGSQPPPARHNGHITPIMPLR"
BASE COUNT          103 a          106 c          111 g           99 t
ORIGIN      
        1 ggattgcccg cgagctacat cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg
       61 gcaacagttc attttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt
      121 catttaccgc agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg
      181 cgctggggca aagactgaat attgaactgg tggcggaggg cgtggaaacg caagaacagg
      241 cgaagtattt gcgccgtcat ggcgtgcata ttttgcaagg gtatttgtac gcacagccga
      301 tgccgctacg cgattttccc aaatggctgg cgggcagcca accgccgccc gcccggcata
      361 atggacatat cacgcccatt atgccgttac gttaacgctg atatgacttt ccgtgtcaa
//
LOCUS       DAJVOY010000430          411 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJVOY010000430 DAJVOY010000000
VERSION     DAJVOY010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.434"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>411
                     /gene="ecpD"
                     /locus_tag="OQL90_004326"
     CDS             <1..>411
                     /gene="ecpD"
                     /locus_tag="OQL90_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HCS1370844.1"
                     /translation="YDASSGARCKDQASGNWYVRNVTHTKAANLRLINTHSLAEVFIN
                     SDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSIHIFPAIANSSLASA
                     VGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADS"
BASE COUNT          109 a          115 c          106 g           81 t
ORIGIN      
        1 tatgacgcca gcagcggcgc gcgttgtaag gatcaggcct ccggcaactg gtatgttcgc
       61 aatgtcaccc atacgaaagc agcaaatctt cggttaataa atacccactc gctggcggag
      121 gtatttatca acagcgacgg ggtgccgacc ctgggcgaag ggaacgccga ctgccggaca
      181 cagaccatcg gcagcctttc aggattaagt tgtaagatgg tcaactatac cctgcaaaca
      241 aacggactca gcaacacctc aatccatata ttcccggcga tcgccaactc gtcgttagcc
      301 tcggccgtcg gggcgtacga tatgcagttc agtctgaatg gcagttcatg gaaaccggtg
      361 agcaataccg cctattacta caccttcaac gagatgaaga gcgcagactc g
//
LOCUS       DAJVOY010000431          411 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJVOY010000431 DAJVOY010000000
VERSION     DAJVOY010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.435"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
BASE COUNT          105 a          108 c           73 g          125 t
ORIGIN      
        1 tggccctgcc tttccagcaa tcgtcgattg ttataccagt ccacccacgt gagtgtggtc
       61 agctcatcat actgacacgt gcattacccc taccgcgata atgcaaccgt acaatatagg
      121 gcgtttccta aacgcagccc tcatcactcc aatccaacaa gacacagcat ttttgatcac
      181 gaagaaagac aaataatttc cgtaaatgtt tccgttgtta caaataacaa cagtgtttta
      241 catttctttg gcctcaggcc cgcgcatgtt gccattcgat gtcattttcc tgactcgttt
      301 cttctttatg gtgtagtgtg atcttcactt tatagatgct aacaatatga aaagaaaaat
      361 tattcccgtg ctaatcggtt gtacgctctc tttctctgcc ctggcggcac a
//
LOCUS       DAJVOY010000432          406 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJVOY010000432 DAJVOY010000000
VERSION     DAJVOY010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.436"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..>406)
                     /locus_tag="OQL90_004327"
     CDS             complement(<1..>406)
                     /locus_tag="OQL90_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1370845.1"
                     /translation="YIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQCIGV
                     EILLRWNNPRQGRISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHVGIN
                     VAPSHFRRGVLIKDLNQYWFSAHPIQQLILEIT"
BASE COUNT          112 a           99 c          106 g           89 t
ORIGIN      
        1 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
       61 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
      121 gactactcat cgggaaaaca tggcgctgac gaatggtttc tgccatcaca tagcgggtca
      181 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccggc
      241 cctgacgcgg attgttccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
      301 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattgattt
      361 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatgta
//
LOCUS       DAJVOY010000433          400 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJVOY010000433 DAJVOY010000000
VERSION     DAJVOY010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.437"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(227..>400)
                     /locus_tag="OQL90_004328"
     CDS             complement(227..>400)
                     /locus_tag="OQL90_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCS1370846.1"
                     /translation="PVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETV
                     SPFPGGKHGLAKV"
BASE COUNT          112 a           87 c           97 g          104 t
ORIGIN      
        1 acagcgcgga caatgaacag taacggtagt catggaaaac ctcaaatacc agtagattac
       61 cagaaacttc tatcgattga agacattacc ttttgccgca ataggtgggg taacattggt
      121 gaaaacgcat tacgccgggt ggcagttgga aaaaaattat cttttttcgg ctctgatcgt
      181 ggtggagaaa atgcggcaat aatctaaata gctgcgctta ataccgctac acttttgcca
      241 gcccatgttt gcctccgggg aatgggctga cggtttccat aaaatggcga acttttttca
      301 acagttgcca cattgagcgg cactgatgat tacgcgttat tgtgtcgtga agtgcctgcc
      361 atagccgttc aacatgattc acccatggcg agtaaaccgg
//
LOCUS       DAJVOY010000434          395 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJVOY010000434 DAJVOY010000000
VERSION     DAJVOY010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.438"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>395
                     /locus_tag="OQL90_004329"
     CDS             <1..>395
                     /locus_tag="OQL90_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCS1370847.1"
                     /translation="RPKDDGYEAKGSALKQHVMAPLISYFRDARAALGITAKQIADAT
                     GKKNMVSHWFSASQWQLPNESDYLKLQALFARVAEEKHRRGELEKLHHQLVDTYTSLN
                     RQYAELLSEYKHLRRYFGVTVQVPYTDVWT"
BASE COUNT          101 a           87 c          124 g           83 t
ORIGIN      
        1 cgcccgaaag atgatggcta tgaggcgaag ggcagtgcac tgaaacagca tgtgatggcc
       61 ccgctgattt cttactttcg tgatgcgcga gctgccctgg ggataacggc aaaacagatt
      121 gcagatgcca caggaaagaa aaacatggtg tcgcactggt tcagtgccag tcagtggcag
      181 ttgccgaatg aaagcgatta tctgaaatta caggcgctgt ttgcccgggt ggcagaagag
      241 aagcatcggc gtggtgaact ggaaaagctc caccaccagc tggtggatac gtatacctca
      301 ctgaaccggc agtatgcgga gctgctgagt gaatataaac atctgcggcg gtattttggc
      361 gtgacggtgc aggtgccgta taccgatgtg tggac
//
LOCUS       DAJVOY010000435          388 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJVOY010000435 DAJVOY010000000
VERSION     DAJVOY010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.439"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..311
                     /locus_tag="OQL90_004330"
     CDS             <1..311
                     /locus_tag="OQL90_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="regulates several genes involved in high affinity
                     phosphate uptake; under conditions of high phosphate
                     concentrations downregulates the PHO regulon; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate transport system regulator PhoU"
                     /protein_id="HCS1370848.1"
                     /translation="HDVLDAFARMDIDEAVRIYREDKKVDQEYEGIVRQLMTYMMEDS
                     RTIPSVLTALFCARSIERIGDRCQNICEFIFYYVKGQDFRHVGGDELDKLLAGKDSDK
                     "
BASE COUNT          104 a           75 c          103 g          106 t
ORIGIN      
        1 tgcacgacgt gctggacgcg ttcgcgcgga tggacattga cgaagcggta cgtatttatc
       61 gtgaagataa aaaagtcgat caggaatacg aaggtattgt tcgtcaactg atgacctaca
      121 tgatggaaga ttcgcgtacc attccgagcg tacttactgc gctgttctgc gcgcgttcta
      181 tcgaacgtat tggcgaccgc tgccagaata tttgtgagtt tatcttctac tacgtgaagg
      241 ggcaggattt ccgtcatgta ggcggtgatg agctggataa actgctggcg ggaaaagata
      301 gcgacaaata attcaccaga caaatcccaa taacttaatt attgggattt gtggtaatgc
      361 tgccaactta ctgatttagt gtatgatg
//
LOCUS       DAJVOY010000436          382 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJVOY010000436 DAJVOY010000000
VERSION     DAJVOY010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.440"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            184..339
                     /locus_tag="OQL90_004331"
                     /pseudo
     CDS             184..339
                     /locus_tag="OQL90_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          120 a           83 c           97 g           82 t
ORIGIN      
        1 gagagcagaa cgggatctat acttttgcgg gtcaacataa gaggatgagt ccttaataaa
       61 acaggtcctc agagaataca tattctgcgg agcgtgcggc aacaacagga cgaacagggg
      121 cggttttcaa tttccagccg tcaggctgcc gtggttcact ccaacaacgg agacaggtaa
      181 attatggacg aaaagaagct taaagccctt gcggctgaac tggctaaagg cctcaaaacc
      241 gaggccgacc tcaatcagtt ttcccgtatg cagacgaaat taactgtcga aacggtgctt
      301 aatgaggaac taactgacca tctcgggcat gaaaaaagct acatccggta gtaaacatta
      361 tcagtgggtt atgcgatatg at
//
LOCUS       DAJVOY010000437          366 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJVOY010000437 DAJVOY010000000
VERSION     DAJVOY010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.441"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..350)
                     /locus_tag="OQL90_004332"
     CDS             complement(<1..350)
                     /locus_tag="OQL90_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370849.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYG"
BASE COUNT          116 a          102 c           87 g           61 t
ORIGIN      
        1 ccgtagtaat cgcacagtgt aacgggtgaa agctcgccgt gtgccacttc atgacgcatg
       61 tagccaatcg agtaaacacc ggtgagaaag ccaatcacac caagaatggc gaggaacaaa
      121 ccgcccagac catcaatatg aagccacagt cccgcagcga atatttctcc tgcatcagcg
      181 gcagtttgga ccacccagag tgccagaatc agcagcagtg tgatccctaa gctatgtaat
      241 acagtcaccg tacaagtcgc agagagcccc cgtttccggc aggcaaaaca gagcagcgaa
      301 aaaagcagcg gcgtgagcag gagtaaagcg aacatcacag aataactcat ctcattatcc
      361 cttcag
//
LOCUS       DAJVOY010000438          361 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJVOY010000438 DAJVOY010000000
VERSION     DAJVOY010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.442"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>361
                     /locus_tag="OQL90_004333"
     CDS             <1..>361
                     /locus_tag="OQL90_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCS1370850.1"
                     /translation="KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHL
                     KGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPR
                     VVKERPWKYPTAPKKSQS"
BASE COUNT          108 a           71 c          111 g           71 t
ORIGIN      
        1 aaacagacga tgcaactgag caggctgacg ctgagaagta aaaagccgga gcttgtggag
       61 caagagctgt ggggtgtctt actggcttat aatctggtga gatatcagat gattaaaatg
      121 gcggaacatc tgaaaggtta ctggccgaat caactgagtt tctcagaatc atgcggaatg
      181 gtgatgagaa tgctgatgac attgcagggc gcttcaccgg gacgtatacc ggagctgatg
      241 cgcgatcttg caagtatggg acaacttgtg aaattaccga cgagaagggg aagggccttc
      301 ccgagagtgg taaaggagag gccctggaaa taccccacag ccccgaaaaa gagccagtca
      361 g
//
LOCUS       DAJVOY010000439          354 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJVOY010000439 DAJVOY010000000
VERSION     DAJVOY010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.443"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            74..>354
                     /locus_tag="OQL90_004334"
     CDS             74..>354
                     /locus_tag="OQL90_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1370851.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRRP"
BASE COUNT           79 a          100 c           83 g           92 t
ORIGIN      
        1 atttaaaccg tctggtctgt ttcctccggc tccacaaaaa taatgtccat cattttgaag
       61 tggacactat cgtatggaat accggacctg gatcactgaa gctttacgcc ttcatttcga
      121 agaacattta ccacgggttg tggccggacg tcgcctgggc gtaccaaaat caactgtctg
      181 tggtatgttc gtgcgcttcc gcaacgctgg cctttcatgg cctctgcccg caggtatgtc
      241 ggagcaggaa cttgatgccc ttctttacgg aagtgcctcc acagtacctg tcgtacttac
      301 tgaaagcacc gttatgccca aactcccggt ggtaaaaaaa cgtccccggc ggcc
//
LOCUS       DAJVOY010000440          348 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJVOY010000440 DAJVOY010000000
VERSION     DAJVOY010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.444"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..216
                     /locus_tag="OQL90_004335"
     CDS             <1..216
                     /locus_tag="OQL90_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS1370852.1"
                     /translation="ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSL
                     DLGYVGESVKHFNPEFVRGCLAVKIHD"
BASE COUNT          101 a           58 c           79 g          110 t
ORIGIN      
        1 attctgcgtt ggaatattca acggggagtg gtcgttattc cgaaatcgac acatcaacag
       61 cgtatcgaag aaaattttgc tatctgggat ttctcactga cagagaaaga aatggcacaa
      121 attagttcgc ttgatttggg ttatgttggg gagtcggtaa aacattttaa tcctgaattt
      181 gttcgtggtt gtcttgctgt aaaaatacat gattgatatt aatcatatat tttacctgag
      241 acgacaagaa tcttttaaca ggggagtgat attgatcttc actctgtcat atctccggta
      301 atatggcgtc agtcttcatc atcagaaagg gggcctgacg cctgaaaa
//
LOCUS       DAJVOY010000441          345 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJVOY010000441 DAJVOY010000000
VERSION     DAJVOY010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.445"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            1..330
                     /locus_tag="OQL90_004336"
                     /pseudo
     CDS             1..330
                     /locus_tag="OQL90_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="multicopy suppressor of dominant negative ftsH
                     mutations; putative acyl-CoA synthetase; incomplete;
                     partial in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT           75 a           87 c          114 g           69 t
ORIGIN      
        1 gatgaaatcc aacttaaaac ccgcgcgcga gaaaaaggct tgctggtgat ggggccggac
       61 tgcggtacgt cgatgattgc cggcacgccg ctggcttttg ctaacgtgat gccggaaggc
      121 aacattggcg tcattggcgc ttccggtacc gggattcagg aactgtgttc gcagattgcg
      181 caggcagggg agggaattac tcacgcgatt ggtcttggcg agcgcgacct cagccgtgaa
      241 gtaggcggca tcagtgcgct gacggcgctg gaaatgctca gtgcagacga gaaaagcgaa
      301 gtgctggcat ttgtttcaaa gccacctgcc tagactggcc ccctg
//
LOCUS       DAJVOY010000442          344 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJVOY010000442 DAJVOY010000000
VERSION     DAJVOY010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.446"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
BASE COUNT           78 a           85 c           90 g           91 t
ORIGIN      
        1 catccgggtg aactggcgtg tttcattagc ccggtagcat gaaccattat ccgtcagtca
       61 cttcactgga gacgccggaa gctcgttgcc gaagcggtgt tccaccgctc ccagcatgtc
      121 gccaacggga gagcatctac ggtctgattg aactgtttat tcaaattgtc caccgtctgt
      181 cagtacgctc tgaacgtcgg ctggttaaaa cactgctcgc tgattttcag agggttcacg
      241 gtaaaacagc acttttattc cggattgcgg aagggctctg aataatccgg atgggctggt
      301 taaagaggtt gtgtaccatc tgcatattgt tgaaccagat cgca
//
LOCUS       DAJVOY010000443          337 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJVOY010000443 DAJVOY010000000
VERSION     DAJVOY010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.447"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            226..>337
                     /gene="ariR"
                     /locus_tag="OQL90_004337"
     CDS             226..>337
                     /gene="ariR"
                     /locus_tag="OQL90_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCS1370853.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQA"
BASE COUNT          121 a           50 c           73 g           93 t
ORIGIN      
        1 taaatttttc attgcagact cttattaagc aattactcgt aatgaaatcg gctgaggaag
       61 atgctttccg acgcgattta attgacagca taattcgtga ttttagcaat agtgattcag
      121 gagggccaaa ccgaagaaca gcgacagctg acaataaaag tatgttcaat ggtaagaaaa
      181 taaacagaat acattaaaat ttcataagta agatgagagg ttaccatgct tgaagatact
      241 acaattcata atgcaataac tgataaagcg ttagcaagtt actttcgcag ttcgggtaat
      301 ttgttagaag aagagtcagc agtgttaggg caggctg
//
LOCUS       DAJVOY010000444          337 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJVOY010000444 DAJVOY010000000
VERSION     DAJVOY010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.448"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
BASE COUNT           96 a           77 c           72 g           92 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattcg
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttggat
//
LOCUS       DAJVOY010000445          333 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJVOY010000445 DAJVOY010000000
VERSION     DAJVOY010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.449"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..214)
                     /gene="atl"
                     /locus_tag="OQL90_004338"
     CDS             complement(<1..214)
                     /gene="atl"
                     /locus_tag="OQL90_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein"
                     /protein_id="HCS1370854.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTG"
BASE COUNT           73 a           88 c           97 g           75 t
ORIGIN      
        1 gtccggttag cgaaattgtg ccgtggcgat taaccacccg gtgccagggt aaggtgctgc
       61 cttcagggag acgctttaac acaccgccca cctggcgcgc ggcgcggggc gatcccgcca
      121 gtttcgccac atcaccgtaa gtggtgacat agccttcggg aatagcggcg acgatttgcc
      181 agacgcgttg gggaaatgaa tcttcttttt ccatcttttc ttcctgaggt aatttttcag
      241 cataatctgg aaaaacgccc gagtgaagtc gcattgcgca agaaaccagc atctggcacg
      301 cgatgggttg caattagccg gggcagcagt gat
//
LOCUS       DAJVOY010000446          331 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJVOY010000446 DAJVOY010000000
VERSION     DAJVOY010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.450"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..292
                     /gene="eno"
                     /locus_tag="OQL90_004339"
     CDS             <1..292
                     /gene="eno"
                     /locus_tag="OQL90_004339"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1370855.1"
                     /translation="NSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATI
                     ADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT           94 a           84 c           76 g           77 t
ORIGIN      
        1 taactccatc ctgatcaaat tcaaccagat cggttctctg accgaaactc tggctgcaat
       61 caagatggcg aaagatgctg gctacactgc agttatctct caccgttctg gcgaaactga
      121 agacgctacc atcgctgacc tggctgttgg tactgctgca ggccagatca aaactggttc
      181 tatgagccgt tctgaccgtg ttgctaaata caaccagctg attcgtatcg aagaagctct
      241 gggcgaaaaa gcaccgtaca acggtcgtaa agagatcaaa ggccaggcat aagactgact
      301 ttatctgatt taaaaatgcc agcctccggg a
//
LOCUS       DAJVOY010000447          326 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJVOY010000447 DAJVOY010000000
VERSION     DAJVOY010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.451"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            complement(<1..261)
                     /locus_tag="OQL90_004340"
     CDS             complement(<1..261)
                     /locus_tag="OQL90_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1370856.1"
                     /translation="MEQKALSAEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDTGVELLPVEITPDEPK"
BASE COUNT           83 a           75 c           79 g           89 t
ORIGIN      
        1 tttcggctca tccggcgtta tctccacagg taataattca acgccagtgt cagaagaggt
       61 tgttaccgga agacgccgcg atatgcgccc ttcgttctgc cagagcctga gccatttgaa
      121 cagcaggtta tcattgatat cgtgttcccg ggcaatacgg gcaacagagg ctcctggttg
      181 tgaagccagt ttaaccattt gaagtttaaa ctcatttgaa aatgatctgc ggggttctgc
      241 agataatgct ttctgttcca taacaggtgt ccactagttg aaaaggtggg cacctacgtt
      301 accaatacag gcttaatggc tacata
//
LOCUS       DAJVOY010000448          303 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJVOY010000448 DAJVOY010000000
VERSION     DAJVOY010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.453"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            226..>303
                     /locus_tag="OQL90_004341"
     CDS             226..>303
                     /locus_tag="OQL90_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370857.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSN"
BASE COUNT           83 a           60 c           59 g          101 t
ORIGIN      
        1 gaatgctgat ttgtaacctt gagtatttat tttccataac atttcctgct ttaacataat
       61 tttccgttaa cataacgggc ttttctcaaa atttcattaa atattgttca cccgttttca
      121 gtacgttacg tttcactgta agaatggaga ttgcccatcc cacaatcctg ggctaagcct
      181 ggaaaggatc aattttcatc cgaacgttcc tgacaggaga aaaccatgag taaggttggt
      241 attaacggtt ttggtcgtat cggtcgactg gtgttgcgtc gattacttga agtcaaaagc
      301 aac
//
LOCUS       DAJVOY010000449          302 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJVOY010000449 DAJVOY010000000
VERSION     DAJVOY010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.denovo.454"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..>302
                     /locus_tag="OQL90_004342"
     CDS             <1..>302
                     /locus_tag="OQL90_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370858.1"
                     /translation="YGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSI
                     AGTGLITKTGSGDLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQD"
BASE COUNT           77 a           67 c           84 g           74 t
ORIGIN      
        1 gctatggaga tggtccttcc actgcggcgg gtggctttat gtatctcggc ttaagtgaag
       61 ttacctttga tattgccgac ggaaaaacgc tggttatcgg caatacagag aatgacggag
      121 ctgttgactc tattgctggt accgggttga tcaccaaaac aggttccggc gatctggtac
      181 ttaatgcaga taacaatgac tttactggcg agatgcagat tgaaaacggt gaagttaccc
      241 tgggccgcag caactccctg atgaatgtcg gcgatacgca ttgccaggac gatccgcaag
      301 ac
//
LOCUS       DAJVOY010000450         6165 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.guided.204, whole genome shotgun sequence.
ACCESSION   DAJVOY010000450 DAJVOY010000000
VERSION     DAJVOY010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.guided.204"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            <1..329
                     /gene="frdB"
                     /locus_tag="OQL90_004343"
     CDS             <1..329
                     /gene="frdB"
                     /locus_tag="OQL90_004343"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1370859.1"
                     /translation="PAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRY
                     NEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLI
                     ATLKPR"
     gene            340..735
                     /gene="frdC"
                     /locus_tag="OQL90_004344"
     CDS             340..735
                     /gene="frdC"
                     /locus_tag="OQL90_004344"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1370860.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            746..1105
                     /gene="frdD"
                     /locus_tag="OQL90_004345"
     CDS             746..1105
                     /gene="frdD"
                     /locus_tag="OQL90_004345"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1370861.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            1168..2301
                     /locus_tag="OQL90_004346"
     CDS             1168..2301
                     /locus_tag="OQL90_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1370862.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2390..2923
                     /gene="blc"
                     /locus_tag="OQL90_004347"
     CDS             2390..2923
                     /gene="blc"
                     /locus_tag="OQL90_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1370863.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2920..3237)
                     /gene="sugE"
                     /locus_tag="OQL90_004348"
     CDS             complement(2920..3237)
                     /gene="sugE"
                     /locus_tag="OQL90_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1370864.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3419..3565)
                     /gene="ecnB"
                     /locus_tag="OQL90_004349"
     CDS             complement(3419..3565)
                     /gene="ecnB"
                     /locus_tag="OQL90_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1370865.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3676..3801)
                     /gene="ecnA"
                     /locus_tag="OQL90_004350"
     CDS             complement(3676..3801)
                     /gene="ecnA"
                     /locus_tag="OQL90_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1370866.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(3853..4419)
                     /gene="efp"
                     /locus_tag="OQL90_004351"
     CDS             complement(3853..4419)
                     /gene="efp"
                     /locus_tag="OQL90_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1370867.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            4461..5510
                     /gene="epmB"
                     /locus_tag="OQL90_004352"
     CDS             4461..5510
                     /gene="epmB"
                     /locus_tag="OQL90_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1370868.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            5884..>6165
                     /locus_tag="OQL90_004353"
     CDS             5884..>6165
                     /locus_tag="OQL90_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370869.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNA"
BASE COUNT         1575 a         1538 c         1541 g         1511 t
ORIGIN      
        1 ccccggcgca gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct
       61 acgccgcgtg cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc
      121 tggcgcatcg ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt
      181 tgaacagcca gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc
      241 cgaaacacgt cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact
      301 ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg
      361 tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg
      421 ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg
      481 tttgccctga aaaatggccc ggacgcctgg gcgggattcg tcgacttttt acaaaacccg
      541 gttatcgtga tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg
      601 tttgaactgg caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag
      661 ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt
      721 gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc
      781 ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat
      841 gatcctgctg gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta
      901 cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat
      961 cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat
     1021 tcacgtgcct gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac
     1081 gctgattggt atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg
     1141 ggccgttttg tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt
     1201 accgcctctt gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca
     1261 attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag
     1321 ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca
     1381 cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc
     1441 gatgctattg ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa
     1501 cttaccgcta aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc
     1561 ggcctgccat tgcagatgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat
     1621 caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc
     1681 ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa
     1741 actcgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc gcccgcagaa
     1801 gaaaagaatt acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc gccaggggcg
     1861 ttagatgctg aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa
     1921 agcaatttaa ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca
     1981 caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac
     2041 tggccggtca atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca
     2101 cgcccggtaa aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa
     2161 acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt
     2221 atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag
     2281 attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct
     2341 tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct
     2401 ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc
     2461 gccgcgtggc gtgacagtgg tgaataattt cgacgctaaa cgctatctcg gtacctggta
     2521 tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata
     2581 cagtctgcgt gatggcggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga
     2641 aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct
     2701 gaaagtgtca ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga
     2761 ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac
     2821 gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt
     2881 tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag
     2941 accaataatc cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc
     3001 accgagcagc acaatgccgg tgatagccgc gccgactgcg ccaatacccg tccacacggc
     3061 ataagccgtc cctactggta acgatttcat cgcccaggca agtagcgcca tactgacaat
     3121 catcgccgtc acggtaataa cactcggcgt caaacgacta aagccgtggg tatatttcag
     3181 gccaacggcc cataccactt ccagcagacc agcaataact aagataatcc aggacatatc
     3241 aggctccgga gaattggggc cgtccccggt gaaaagacgc gtttgcaggt cgtcctgcaa
     3301 agctgatggt tgaacggata ttttgccagg tggcagggga atttcaacta ttaccttagt
     3361 gactatcgca gaaaaaacaa acggcacgac acagctgtcg taccgttatt gcttattgct
     3421 attgctgcgc tttcgttgct gcgccagaaa tcgcgttacc gccatcagaa atgtcttcac
     3481 caacgccacg cgtggtgttg caggcagtta atactgttga aagcaccaga acagaaaaga
     3541 tcgctgcaat tgtcttcttc accataacgt cttcctttta ggtaatgtta tttatgtttg
     3601 cctatagcaa attaataata gacaacatcg ccaaaaatga cggaaatctg atgatttttc
     3661 ggaagagaag aaaaattagc tggcagcgcg agagatggag ttgccgagat gtttgatgtc
     3721 ttcgccgaaa ccgcgagcgg tgttacagcc cgtgagcagc gtactggcaa gcaaaacaag
     3781 aacgataagg cgtttcatca tctctgacct gaataagtga tggtgcagcc tgcaggccgc
     3841 accacaaccg cattacttca cgcgagagac gtattcacca gagcgggtat ccactttgat
     3901 gacttcgccg atttgtacga acagcggaac cttaaccaca gcgccagtag acagggtagc
     3961 cggtttgccg ccagtacctg cggtatcacc tttcaggccc ggatcggtat caacgatttc
     4021 cagttcaacg aagttcggcg gagtaacgga gatcggctga ccattccaca gagttacgat
     4081 acactctgcc tgatccagca gccatttagc gttatcgcca attgcttttg catcagcaga
     4141 cagctgctcg aaagtttcgt tgttcatgaa gtgccagaac tcaccgtcgt tgtacaggta
     4201 agtcaggttc atatcgacaa catcagcgcc ttcagcggaa tcagtagatt tgaaggtttt
     4261 ttctacgcga gtaccggtca gcagacgacg cagtttaacg cgagcaaatg cctggccttt
     4321 acccggtttt acgaattcac tcgcttcaac cgcgtaaggt tcgccgtcta acatgatttt
     4381 aagaccagca cgaaaatcgt tgctatagta cgttgccata aggccctctg aaatttgtta
     4441 attggtagct aagccacaaa atggcgcata ttgtaaccct aaatacccca tccagagaag
     4501 attggttaac gcaacttgcc gatgttgtga ccgatcctga tgaacttctg cgtcttttga
     4561 atatagacgc ggacgaaaaa ctgttggccg gacgcagcgc caaaaagctg tttgccctgc
     4621 gtgtgccccg ctcatttatc gatcgcatgg agaaaggcaa tccggacgat cctcttttgc
     4681 gtcaggtact tacctcgcaa gatgagtttg tcgtcgcgtc cggattctcc accgacccac
     4741 tggaagaaca gcacagcgta gtgcctggtt tgttgcataa ataccacaac cgggcgcttt
     4801 tgctggtcaa aggcggctgc gcggtaaatt gccgctattg cttccgtcgc cacttcccct
     4861 atgccgaaaa tcagggcaac aagcgtaact ggcagactgc acttgagtat gttgctgcgc
     4921 atccggaact ggacgagatg attttctccg gcggcgatcc gctgatggcg aaagatcacg
     4981 agctggactg gttgctcaca caactggaag ccatcccgca tatcaaacgt ctgcggattc
     5041 acagccgtct gccgattgtg atcccggcac gcatcacaga tgcgctggtt gaacgctttt
     5101 cacattctac gctgcaaatc ctgctggtga atcacatcaa ccatgccaat gagatagatg
     5161 aaacattccg tcaggcgatg gctaagttgc gccgggtagg cgttactttg ctgaaccaga
     5221 gcgttctgtt acgtgatgtg aacgataacg cacaaacgct ggcaaacctg agtaatgcgt
     5281 tgttcgatgc cggcgtgatg ccgtattacc tgcatgtgct cgataaagta cagggcgcag
     5341 cgcattttat ggtgagtgat gacgaagcac ggcagattat gcgtgagttg ctgacattgg
     5401 tgtcgggata tctggtgccg aaactggcgc gagaaattgg cggcgaaccc agcaaaacgc
     5461 cgctggatct ccagctacgc cagcagttac tgattaaaca gggaagttaa acattaaaat
     5521 tgcctgatgc gctacgctta tcaggcctac atcgtgcatt gaaattgaat ggatttgcaa
     5581 tgaactgtag gccggataag gcgtttacgc cgcatccggc aagagcaaca aacgcaagta
     5641 cagcaacctg tcattaaact gagaaattta atctttgccc gttgcataac ctttaattat
     5701 tcatatgcat tgctgttgtt actttttata tagctaacac caataattaa cctttctttt
     5761 ctgtttatat agagtgagca cggctattta tgttatttta acaactatta ctaccaagcc
     5821 catttatctg gcttataaat taaaattaat tatttaattg agaaatggtt agggagaacc
     5881 tacatggcca taagcatcaa gggtgttaat accggtgtta ttcgcaagag taataatttc
     5941 attgcactgg cgctgaaaat taaagaacct cgcaataaag aatcgttgtt ctttatgtct
     6001 gtcatggaac tgcgcgacct gctgattgcg ctggaaagtc gtctgcatca aaagcataaa
     6061 ctggatgctg ctgcacgcct tcagtatgag caagctcgcg ataaagtcat taagaaaatg
     6121 gcagaaaaca tcccagaaat tctggttgat gagcttaaaa atgcc
//
LOCUS       DAJVOY010000451         6153 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.guided.206, whole genome shotgun sequence.
ACCESSION   DAJVOY010000451 DAJVOY010000000
VERSION     DAJVOY010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.guided.206"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            798..1613
                     /gene="sul2"
                     /locus_tag="OQL90_004354"
     CDS             798..1613
                     /gene="sul2"
                     /locus_tag="OQL90_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1370870.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1674..2201
                     /locus_tag="OQL90_004355"
                     /pseudo
     CDS             1674..2201
                     /locus_tag="OQL90_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2222..2695
                     /locus_tag="OQL90_004356"
     CDS             2222..2695
                     /locus_tag="OQL90_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCS1370871.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2770..3045
                     /locus_tag="OQL90_004357"
                     /pseudo
     CDS             2770..3045
                     /locus_tag="OQL90_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            3045..3881
                     /locus_tag="OQL90_004358"
     CDS             3045..3881
                     /locus_tag="OQL90_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1370872.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3988..4392)
                     /locus_tag="OQL90_004359"
                     /pseudo
     CDS             complement(3988..4392)
                     /locus_tag="OQL90_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4436..4825)
                     /locus_tag="OQL90_004360"
     CDS             complement(4436..4825)
                     /locus_tag="OQL90_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1370873.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            5043..5483
                     /locus_tag="OQL90_004361"
     CDS             5043..5483
                     /locus_tag="OQL90_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1370874.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1492 a         1620 c         1590 g         1451 t
ORIGIN      
        1 ggtggcgaaa cccgacagga ctataaagat cccaggcgtt tccccctggt agctccctcg
       61 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcggt
      121 ttctcattcc acgcctgaca ctcagttccg tgaaggcagt tcgctccaag ctggactgta
      181 tgcacgaacc ccccgttcag tccgaccgct gcgccttgtc cggtaactat caacttgagt
      241 ccaacccgga aagacacgac aaaacgccac tggcagcagc cattagtaac aggagtgcac
      301 gagaagatag attaagaggt cttgaagtag aggcctgact gcggctacac tgaaagaaca
      361 gttttggtaa tggcggtctc gtgaaccctg ttaccgggtt aagcagttcc ccaactgact
      421 taaccttcga acaaaccgcc tccccaggcg gttttttcgt ttacagagca agagattacg
      481 attcattgaa aaggatctca agaagatcct ttgtagatta ctatcaatta atcttccgaa
      541 catgacacca gtcactgttg attacggtta aaccgctccg taacaggttt ccctcgtatt
      601 ggtgacttgc agcaccaaag aagcaaaaaa gataaatata tctacttatt gagagatgat
      661 atatttattt ccaaaatgct acagatacat catcatgaaa cggatcaccg caaacaggtt
      721 actcgcctgg ggattccctt tcgacccgag catccgtatg agactcatgc tcgattatta
      781 ttattataga agcccccatg aataaatcgc tcatcatttt cggcatcgtc aacataacct
      841 cggacagttt ctccgatgga ggccggtatc tggcgccaga cgcagccatt gcgcaggcgc
      901 gtaagctgat ggccgagggg gcagatgtga tcgacctcgg tccggcatcc agcaatcccg
      961 acgccgcgcc tgtttcgtcc gacacagaaa tcgcgcgtat cgcgccggtg ctggacgcgc
     1021 tcaaggcaga tggcattccc gtctcgctcg acagttatca acccgcgacg caagcctatg
     1081 ccttgtcgcg tggtgtggcc tatctcaatg atattcgcgg ttttccagac gctgcgttct
     1141 atccgcaatt ggcgaaatca tctgccaaac tcgtcgttat gcattcggtg caagacgggc
     1201 aggcagatcg gcgcgaggca cccgctggcg acatcatgga tcacattgcg gcgttctttg
     1261 acgcgcgcat cgcggcgctg acgggtgccg gtatcaaacg caaccgcctt gtccttgatc
     1321 ccggcatggg gttttttctg ggggctgctc ccgaaacctc gctctcggtg ctggcgcggt
     1381 tcgatgaatt gcggctgcgc ttcgatttgc cggtgcttct gtctgtttcg cgcaaatcct
     1441 ttctgcgcgc gctcacaggc cgtggtccgg gggatgtcgg ggccgcgaca ctcgctgcag
     1501 agcttgccgc cgccgcaggt ggagctgact tcatccgcac acacgagccg cgccccttgc
     1561 gcgacgggct ggcggtattg gcggcgctga aagaaaccgc aagaattcgt taactgcaca
     1621 ttcgggatat ttctctatat tcgcgcttca tcagaaaact gaaggaacct ccattgaatc
     1681 gaactaatat tttttttggt gaatcgcatt ctgactggtt gcctgtcaga ggcggagaat
     1741 ctggtgattt tgtttttcga cgtggtgacg ggcatgcctt cgcgaaaatc gcacctgctt
     1801 cccgccgcgg tgagctcgct ggagagcgtg accgcctcat ttggctcaaa ggtcgaggtg
     1861 tggcttgccc cgaggtcatc aactggcagg aggaacagga gggtgcatgc ttggtgataa
     1921 cggcaattcc gggagtaccg gcggctgatc tgtctggagc ggatttgctc aaagcgtggc
     1981 cgtcaatggg gcagcaactt ggcgctgttc acagcctatc ggttgatcaa tgtccgtttg
     2041 agcgcaggct gtcgcgaatg ttcggacgcg ccgttgatgt ggtgtcccgc aatgccgtca
     2101 atcccgactt cttaccggac gaggacaaga gtacgccgca gctcgatctt ttggctcgtg
     2161 tcgaactaga gctaccggtg cggctcgacc aagagcgcac cgttaaccca ggatgagaac
     2221 cttgaaagta tcattgatag ctgcgaaagc gaaaaacggc gtgattggtt gcggtccaga
     2281 cataccctgg tccgcgaaag gggagcagct actttttaaa gcattgacct acaatcagtg
     2341 gcttctggtg ggtcgcaaga cgtttgaatc tatgggcgca ctccccaata ggaaatacgc
     2401 ggtcgttacc cgctcaggtt ggacatcaaa tgatgacaat gtagttgtat ttcagtcaat
     2461 cgaagaggcc atggacaggc tagctgaatt caccggtcac gttatagtgt ctggtggcgg
     2521 agaaatttac cgagaaacat tacccatggc ctctacgctc cacttatcga cgatcgacat
     2581 cgagccagag ggggatgttt tcttcccgag tattccaaat accttcgaag ttgtttttga
     2641 gcaacacttt acttcaaaca ttaactattg ctatcaaatt tggaaaaagg gttaacaaag
     2701 ctatgcaatc gacggcaaaa agcttcgttc gcttcgcgca ctacgccttt ttccgcgatt
     2761 gatagcgacg atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac
     2821 cctaaaactc ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc
     2881 tatgccgatt tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa
     2941 gcagagcgcg ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa
     3001 cgccttgcct tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg
     3061 tttttcctgc tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc
     3121 tcgtttggaa agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta
     3181 tagaagacat tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg
     3241 gagcagtccg gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc
     3301 gaatgctctc tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg
     3361 aactaatggc gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga
     3421 tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc
     3481 aaactgacta cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac
     3541 tgcgtgggct acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc
     3601 tggtgataga tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct
     3661 acgatccggc tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg
     3721 acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt
     3781 atgggtgcct ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta
     3841 tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct
     3901 atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg
     3961 attccgcccc ttcccgcaaa cgaccccaaa caggaaacgc agctgaaacg ggaagctcaa
     4021 cacccactga cgcatgggtt gttcaggcag tacttcatca accagcaagg cggcactttc
     4081 ggccatccgc cgcgccccac agctcgggca gaaaccgcga cgcttacagc tgaaagcgac
     4141 caggtgctcg gcgtggcaag actcgcagcg aacccgtaga aagccatgct ccagccgccc
     4201 gcattggaga aattcttcaa attcccgttg cacatagccc ggcaattcct ttccctgctc
     4261 tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg atctgataga gaagggtttg
     4321 ctcgggtcgg tggctctggt aacgaccagt atcccgatcc cggctggccg tcctggccgc
     4381 cacatgaggc atgttccgcg tccttgcaat actgtgttta catacagtct atcgcttagc
     4441 ggaaagttct tttaccctca gccgaaatgc ctgccgttgc tagacattgc cagccagtgc
     4501 ccgtcactcc cactcttaca ttgttcgcag tatctaggta catgttattg cgtgcacctt
     4561 catgatgtat aatgttatta atgacagcag aaagatcgac agaattaata ttcttcgtac
     4621 ctgggacagt tatcgggtgt cggccagtga tgagaccaaa ggatgcagta taagatgaac
     4681 cactactatt atcatggcca ccattaccac caccgttaaa actacctccc gaacttccag
     4741 agccgctatg accatcattg taggggtcat ttggtcccat gtgaccaaaa ccaccgacag
     4801 taccgctatt taactctgta ctcataaaat tatcctcttt gacttttaaa aacagcaaaa
     4861 actgtattta taacaatgtg tttatataca gtattcgaaa tactacgccc gtcaaaacca
     4921 caccgtcaac aggttttttg tgtatttttc acgcctcacg ggcagcctca gaccttttgt
     4981 gttaaaacag taatatgttc tgctgttaca atacggtaca cacaggagac actgtcactt
     5041 ttatggcatg gcacacagtt caagagacac tgaaattaac aggaaagtcg cgctcgcaat
     5101 tatatcgtga catggctaaa ggccttgtca gctatcgcac aggaaaaaat gatcgccggg
     5161 agttcgagac cagtgaactg attcgggcct atggcgagct gcgcacttct gagacaccaa
     5221 aaaggcacaa ggagggacac tccaaatctg tcgttagcca ggcggaaaat gctcagctag
     5281 acgtcattca gcagaaactg gacgctctgc agcagactgt taccctaatg cttgaagaca
     5341 agatggcgcg ggaagcagaa aaccgcctgc gaaatgagga gagggaacaa ctacaggccg
     5401 aaattagccg tctggctgac gcgctggcac aggaaaaaaa gcggggattc tggtcgcggc
     5461 tgtttcgctc cggttcagag taacagtgca ggtgactctg atttaccata acatctgtta
     5521 tcagcaccgc ctgagaggcc ctcagggcgt ttctgacggg aaaccggtaa aacaggcaac
     5581 aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat gcatgagtat
     5641 gcgcacaagt gcatagcgcg gaaattcaca ccattcagcc ggggtaagtc ctgtttccgg
     5701 cgttcttccc atccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt
     5761 tgccggcgca gccggaaaac ccgctgtcag cacgttcagg aagggggtga tgtacaaatg
     5821 gcacagagga tgaaagtgac gtatgagctg aaggaatgat gtaaaagtga tgtaaaagtg
     5881 atgtataagt gatgtaaaag tgatgtatga actgaaggag cgatgtaatt ctgtagtcag
     5941 gaagaataac ccgttcatgc aggcatgaac gatacagtga ggaacgcttt ttttgtcacg
     6001 atctcccctt aacctgcgta aatcacccgc catggttgat tatttttcaa cctcaaaatg
     6061 gtcatacacc taacgcagcc gcgattttca tcgcaactgc atgaattaaa agaaaaaaaa
     6121 ggaatatttc gttttcacga gtaatacccg ggt
//
LOCUS       DAJVOY010000452         4887 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.guided.232, whole genome shotgun sequence.
ACCESSION   DAJVOY010000452 DAJVOY010000000
VERSION     DAJVOY010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.guided.232"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            175..579
                     /locus_tag="OQL90_004362"
     CDS             175..579
                     /locus_tag="OQL90_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCS1370875.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(557..1180)
                     /gene="tetR(B)"
                     /locus_tag="OQL90_004363"
     CDS             complement(557..1180)
                     /gene="tetR(B)"
                     /locus_tag="OQL90_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1370876.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1262..2467
                     /gene="tet(B)"
                     /locus_tag="OQL90_004364"
     CDS             1262..2467
                     /gene="tet(B)"
                     /locus_tag="OQL90_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1370877.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2580..3173)
                     /gene="tetC"
                     /locus_tag="OQL90_004365"
     CDS             complement(2580..3173)
                     /gene="tetC"
                     /locus_tag="OQL90_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1370878.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3306..3677
                     /locus_tag="OQL90_004366"
     CDS             3306..3677
                     /locus_tag="OQL90_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1370879.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3687..>4887)
                     /locus_tag="OQL90_004367"
     CDS             complement(3687..>4887)
                     /locus_tag="OQL90_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCS1370880.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1313 a          924 c         1048 g         1602 t
ORIGIN      
        1 aaaacactat caataagttg gagtcattac cctgtggcga atatatcagc ttcaacggcg
       61 agcagtcatt tatctaaatt attagattgc cagctaatca cagtagtagc tcaaggcaag
      121 catcgttatt ttcggctagc aggaaaagat attgctgaat tgatggaaag tatgatgggg
      181 atctccttaa accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca
      241 cgtacttgct atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt
      301 caacagcaat ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt
      361 catgaaatgg gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta
      421 gattggagtg aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt
      481 tatgaatcta aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa
      541 aaaggttatg ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata
      601 tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga
      661 tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag
      721 cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga
      781 gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt
      841 cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc
      901 gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc
      961 tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg
     1021 cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta
     1081 gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta
     1141 atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc taatttttgt
     1201 tgacactcta tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga
     1261 aatgaatagt tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg
     1321 ccttatcatg ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc
     1381 taaccacttt ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg
     1441 gcttggaaaa atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg
     1501 cgcatcgctg gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg
     1561 ccgtttgctt tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga
     1621 taccacctca gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct
     1681 tggtttaata gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc
     1741 cttttttatc gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg
     1801 tgaaaccaaa aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa
     1861 ttcggtatac atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc
     1921 gcaattgata ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg
     1981 atggaatagc atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt
     2041 ccaagccttt gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct
     2101 cggatttatt gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt
     2161 agttttccct gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg
     2221 agtgatgtct atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag
     2281 ccttaccaat gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc
     2341 actaccaatt tgggatggct ggatttggat tattggttta gcgttttact gtattattat
     2401 cctgctatcg atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag
     2461 tgcttagtta tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata
     2521 acccaagagg gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt
     2581 tatttatctt tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta
     2641 tcggcaagct cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt
     2701 aagtttttaa gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg
     2761 acaatggcgt ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa
     2821 ccaatgatag gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa
     2881 tcaagataat gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt
     2941 gttgtgatgt aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt
     3001 tttttatttt taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc
     3061 tcatctattg aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc
     3121 agtgattgat aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat
     3181 ttaaaataac ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt
     3241 aagaacattt aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta
     3301 ataacatcca attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc
     3361 tattatggat tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag
     3421 cgggttatac caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg
     3481 ctagctatat tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa
     3541 aaactatcct tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc
     3601 gatttaagta catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat
     3661 tggaggcaat gcactgagag atcccctcat aatttcccca aagcgtaacc atgtgtgaat
     3721 aaattttgag ctagtagggt tgcagccacg agtaagtctt cccttgttat tgtgtagcca
     3781 gaatgccgca aaacttccat gcctaagcga actgttgaga gtacgtttcg atttctgact
     3841 gtgttagcct ggaagtgctt gtcccaacct tgtttctgag catgaacgcc cgcaagccaa
     3901 catgttagtt gaagcatcag ggcgattagc agcatgatat caaaacgctc tgagctgctc
     3961 gttcggctat ggcgtaggcc tagtccgtag gcaggacttt tcaagtctcg gaaggtttct
     4021 tcaatctgca ttcgcttcga atagatatta acaagttgtt tgggtgttcg aatttcaaca
     4081 ggtaagttag ttgctagaac ccatggctcc tttgccgacg ctgagtagat tttaggtgac
     4141 gggtggtgac aatgagtccg tgtcgagcgc tgattttttc ggcctttaga gcgagattta
     4201 tacaatagaa tttggcatga gattggattg cttttagtca gcctcttata gcctaaagtc
     4261 tttgagtgac tagatgacat atcatgtaag ttgctgatag gtttccagtt ttccgctcct
     4321 aggtctgcat attgtacttt tcctcttact cgacttaacc agtaccaacc cagcttctca
     4381 acggatttat accatggcac tttaaagcca gcatcactga caatgagcgg tgtggtgtta
     4441 ctcggtagaa tgctcgcaag gtcggctaga aattggtcat gagctttctt tgaacattgc
     4501 tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac gaccgtgtag tgcgactgaa
     4561 gctcgcaata ccataagtcg tttttgctca cgaatatcag accagtcaac aagtacaatg
     4621 ggcatcgtat tgcccgaaca gataaagcta gcatgccaac ggtatacagc gagtcgctct
     4681 ttgtggaggt gacgattacc taacaatcgg tcgattcgtt tgatgttatg ttttgttctc
     4741 gctttggttg gcaggttacg gccaagttcg gtaagagtga gagttttaca gtcaagtaat
     4801 gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt gtaattcggg gtaatgactc
     4861 caacttactg atagtgtttt atgttca
//
LOCUS       DAJVOY010000453         2292 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 604423
            SAMEA8900859-rid15852803.guided.300, whole genome shotgun sequence.
ACCESSION   DAJVOY010000453 DAJVOY010000000
VERSION     DAJVOY010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900859
            Sequence Read Archive: ERR6005342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478256.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478256.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:23:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,370
            CDSs (total)                      :: 4,300
            Genes (coding)                    :: 3,966
            CDSs (with protein)               :: 3,966
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 62
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 181 of 334
            Pseudo Genes (incomplete)         :: 91 of 334
            Pseudo Genes (internal stop)      :: 98 of 334
            Pseudo Genes (multiple problems)  :: 35 of 334
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900859-rid15852803.guided.300"
                     /serovar="S. flexneri 6"
                     /isolate="604423"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-10-13"
     gene            197..655
                     /gene="gpt"
                     /locus_tag="OQL90_004368"
     CDS             197..655
                     /gene="gpt"
                     /locus_tag="OQL90_004368"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1370881.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            747..1991
                     /gene="frsA"
                     /locus_tag="OQL90_004369"
     CDS             747..1991
                     /gene="frsA"
                     /locus_tag="OQL90_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1370882.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2049..>2292
                     /gene="crl"
                     /locus_tag="OQL90_004370"
     CDS             2049..>2292
                     /gene="crl"
                     /locus_tag="OQL90_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1370883.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGD"
BASE COUNT          547 a          559 c          609 g          577 t
ORIGIN      
        1 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
       61 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
      121 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
      181 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
      241 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
      301 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
      361 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgagagctta aagtgctgaa
      421 acgcgcagaa ggcgatggcg aaggcttcat agttattgat gacctggtgg ataccggtgg
      481 tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
      541 accggctggt cgtccgctgg ttgatgacta tgttgttgat attccgcaag atacctggat
      601 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
      661 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcaggtta caatagtttc
      721 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
      781 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
      841 aaccgcctgt gcagtcggct cttgatggta aaaccatccc tcactggtat cgcatgatta
      901 accgtctgat gtggatctgg cgcggtattg acccacgtga aatcctcgac gtccaggcac
      961 gtattgtgat gagcgatgct gaacgtaccg acgatgattt atacgatact gtgattggct
     1021 accgtggtgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
     1081 cctgtgcgga agaagatccg caactcagtg gtcgacactg gctgcatgcg gctacgttgt
     1141 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     1201 tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc gggcacgatg cggcagatgg
     1261 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     1321 gcccgttccc gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     1381 gcctgtatga acgttatttt gcgccgcgcg gcattgtgat gctgactatt gatatgccgt
     1441 cggtgggctt ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg
     1501 tcttaaaggc gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     1561 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag
     1621 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     1681 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
     1741 acgaagcgtt acgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctgggac
     1801 gtcgctgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     1861 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
     1921 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac
     1981 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg
     2041 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     2101 cactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     2161 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgaattctgg ggctggtgga
     2221 tggagctgga agcgcaggaa tcccgtttta cctacagcta ccagtttggt ctgttcgata
     2281 aagcaggcga ct
//
